Comment[ArrayExpressAccession]	E-GEOD-42903
MAGE-TAB Version	1.1							
Public Release Date	2012-12-14
Investigation Title	Arsenic trioxide inhibits the metastasis of osteosarcoma through suppressing transcriptional activity of GLI2 and downregulation of ribosomal protein S3							
Comment[Submitted Name]	Arsenic trioxide inhibits the metastasis of osteosarcoma through suppressing transcriptional activity of GLI2 and downregulation of ribosomal protein S3
Experiment Description	Aberrations in the Hedgehog (Hh) pathway are known to related to several malignancies. However, little is known about the function of GLI2, a transcription factor in the Hh pathway, in osteosarcoma. Osteosarcoma is the most frequent primary bone sarcoma in children and adolescents. Despite survival rates of osteosarcoma patients have increased, the prognosis of patients with metastasis remains poor. Therefore, the development of novel therapeutic strategies for osteosarcoma patients is development of novel therapeutic strategies for osteosarcoma patients is urgently needed. Aberrations in the Hedgehog (Hh) pathway are known to related to several malignancies. However, little is known about the function of GLI2, a transcription factor in the Hh pathway, in osteosarcoma. Our findings revealed that GLI2 was overexpressed in osteosarcoma tissues. Additionally, GLI2 is involved in the metastasis of osteosarcoma cells through the regulation of ribosomal protein S3 expression. Furthermore, we showed that arsenic trioxide (ATO) suppressed the invasion and lung metastasis of osteosarcoma cells by the inhibition of GLI transcription. Consequently, these finding reveal a novel function of GLI2 in the metastasis of osteosarcoma and that ATO may be a new therapeutic agentay be a new therapeutic agent. We revealed that a novel function of GLI2 in the metastasis of osteosarcoma and that ATO may be a new therapeutic agent for preventing osteosarcoma metastasis. Negative siRNA(U-2OS) and GLI2 siRNA(U-2OS)							
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl						
Person Last Name	Nagao	Nagao	Setoguchi					
Person First Name	Hiroko	Hiroko	Takao					
Person Email	geo@ncbi.nlm.nih.gov							
Person Affiliation	Kagoshima University							
Person Address	Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, Japan							
Person Roles	submitter							
Protocol Name	P-GSE42903-1	P-GSE42903-6	P-GSE42903-3	P-GSE42903-8	P-GSE42903-7	P-GSE42903-2	P-GSE42903-4	P-GSE42903-5
Protocol Description	ID_REF =  VALUE = MAS5.0 signal intensity	Following fragmentation, 5.5 ug of ssDNA were hybridized for 16 hr at 45C on Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.	U-2OS cell lines were cultured in DMEM at 37°C in 5% CO2.	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.	U-2OS cells were treated with siRNA for 48 hr.	Total RNA was extracted from the cell lines and tissue specimens using miR-Vana RNA isolation kits.	Second-Strand cDNA (ssDNA) were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Protocol Type	bioassay_data_transformation	hybridization	grow	feature_extraction	image_aquisition	specified_biomaterial_action	nucleic_acid_extraction	labeling
Comment[SecondaryAccession]	GSE42903							
Comment[GEOReleaseDate]	2012-12-14							
Comment[ArrayExpressSubmissionDate]	2012-12-13							
Comment[GEOLastUpdateDate]	2012-12-16							
Comment[AEExperimentType]	transcription profiling by array							
SDRF File	E-GEOD-42903.sdrf.txt