Comment[ArrayExpressAccession]	E-GEOD-42603
MAGE-TAB Version	1.1						
Public Release Date	2013-06-01
Investigation Title	The data of differentially regulated exons from mouse testes - wild-type and Dazap1 mutant						
Comment[Submitted Name]	The data of differentially regulated exons from mouse testes - wild-type and Dazap1 mutant
Experiment Description	The binding of Deleted in Azoospermia Associated Protein 1 (DAZAP1) to splicing mutations in the BRCA1 and NF1 genes that caused exon exclusion suggest a role for DAZAP1 in RNA splicing. To elucidate the biological functions of DAZAP1 and to search for its natural RNA substrates, we compared the exon usages of wild-type and Dazap1 mutant testes by exon microarrays. We used wild-type and Dazap1 mutant mouse testes. Each genotype has three replicates. 6 total samples were analyzed.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Chen	Chen	Yen				
Person First Name	Hsiang-Ying	Hsiang	Pauline				
Person Mid Initials		Y					
Person Email	geo@ncbi.nlm.nih.gov						
Person Affiliation	IBMS						
Person Address	IBMS, 128 Sec. 2, Academia Rd., Taipei, Taiwan						
Person Roles	submitter						
Protocol Name	P-GSE42603-1	P-GSE42603-5	P-GSE42603-6	P-GSE42603-2	P-GSE42603-3	P-GSE42603-4	P-GSE42603-7
Protocol Description	Data were processed using EASANA from GenoSplice technology. GC background correction were applied to probe intensities (core). Exon-level expression were summarized from the corrected intensities and then quantile normalized. Probe group file: MoEx-1_0-st-v1.r2.pgf Meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm8.mps ID_REF =  VALUE = Log2 quantile-normalized exon-level expression value from EASANA	Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix).	Samples were hybridized using Affymetrix hybridization kit materials. Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes, and centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). Transfer 200μl of hyb solution to each array, then tape holes and parafilm. Hybridize 16 hours at 45° at 60rpm. Fluidics washing is FS450.	Not applicable.	Testes were dissected from P21 and adult mice.	All RNA samples were prepared using Trizol. The cDNA synthesis and amplification were performed using the WT cDNA synthesis and amplification kit (Affymetrix).	Affymetrix GeneChIP Scanner 3000.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	GENOTYPE	AGE					
Experimental Factor Type	genotype	age					
Comment[SecondaryAccession]	GSE42603						
Comment[GEOReleaseDate]	2013-06-01						
Comment[ArrayExpressSubmissionDate]	2012-11-28						
Comment[GEOLastUpdateDate]	2013-06-02						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-42603.sdrf.txt