Source Name Comment [Sample_description] Comment [Sample_source_name] Comment [Sample_title] Characteristics [organism] Term Source REF Term Accession Number Extract Name Material Type Labeled Extract Name Label Assay Name Array Design REF Term Source REF Technology Type Array Data File Comment [ArrayExpress FTP file] Protocol REF Term Source REF Normalization Name Derived Array Data File Comment [Derived ArrayExpress FTP file] GSM96285 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T4_R3, Monolayer Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96285 extract 1 total RNA GSM96285 LE 1 1 GSM96285 A-AFFY-44 ArrayExpress array assay GSM96285.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96285_sample_table.txt norm GSM96285_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96284 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T4_R2, Monolayer Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96284 extract 1 total RNA GSM96284 LE 1 1 GSM96284 A-AFFY-44 ArrayExpress array assay GSM96284.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96284_sample_table.txt norm GSM96284_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96283 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T4_R1, Monolayer Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96283 extract 1 total RNA GSM96283 LE 1 1 GSM96283 A-AFFY-44 ArrayExpress array assay GSM96283.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96283_sample_table.txt norm GSM96283_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96282 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T3_R3, Spheroid Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96282 extract 1 total RNA GSM96282 LE 1 1 GSM96282 A-AFFY-44 ArrayExpress array assay GSM96282.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96282_sample_table.txt norm GSM96282_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96281 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T3_R2, Spheroid Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96281 extract 1 total RNA GSM96281 LE 1 1 GSM96281 A-AFFY-44 ArrayExpress array assay GSM96281.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96281_sample_table.txt norm GSM96281_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96280 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T3_R1, Spheroid Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96280 extract 1 total RNA GSM96280 LE 1 1 GSM96280 A-AFFY-44 ArrayExpress array assay GSM96280.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96280_sample_table.txt norm GSM96280_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96279 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T2_R3, Spheroid Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96279 extract 1 total RNA GSM96279 LE 1 1 GSM96279 A-AFFY-44 ArrayExpress array assay GSM96279.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96279_sample_table.txt norm GSM96279_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96278 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T2_R2, Spheroid Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96278 extract 1 total RNA GSM96278 LE 1 1 GSM96278 A-AFFY-44 ArrayExpress array assay GSM96278.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96278_sample_table.txt norm GSM96278_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96277 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T2_R1, Spheroid Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96277 extract 1 total RNA GSM96277 LE 1 1 GSM96277 A-AFFY-44 ArrayExpress array assay GSM96277.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96277_sample_table.txt norm GSM96277_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96276 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T1_R3, Monolayer Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96276 extract 1 total RNA GSM96276 LE 1 1 GSM96276 A-AFFY-44 ArrayExpress array assay GSM96276.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96276_sample_table.txt norm GSM96276_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96275 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T1_R2, Monolayer Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96275 extract 1 total RNA GSM96275 LE 1 1 GSM96275 A-AFFY-44 ArrayExpress array assay GSM96275.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96275_sample_table.txt norm GSM96275_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96274 1 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human T98G Glioma Cancer Cells T98G_T1_R1, Monolayer Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96274 extract 1 total RNA GSM96274 LE 1 1 GSM96274 A-AFFY-44 ArrayExpress array assay GSM96274.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96274_sample_table.txt norm GSM96274_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96273 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T4_R3, Monolayer Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96273 extract 1 total RNA GSM96273 LE 1 1 GSM96273 A-AFFY-44 ArrayExpress array assay GSM96273.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96273_sample_table.txt norm GSM96273_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96272 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T4_R2, Monolayer Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96272 extract 1 total RNA GSM96272 LE 1 1 GSM96272 A-AFFY-44 ArrayExpress array assay GSM96272.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96272_sample_table.txt norm GSM96272_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96271 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T4_R1, Monolayer Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96271 extract 1 total RNA GSM96271 LE 1 1 GSM96271 A-AFFY-44 ArrayExpress array assay GSM96271.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96271_sample_table.txt norm GSM96271_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96270 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T3_R3, Spheroid Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96270 extract 1 total RNA GSM96270 LE 1 1 GSM96270 A-AFFY-44 ArrayExpress array assay GSM96270.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.1.zip P-GSE4219-1 ArrayExpress GSM96270_sample_table.txt norm GSM96270_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96269 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T3_R2, Spheroid Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96269 extract 1 total RNA GSM96269 LE 1 1 GSM96269 A-AFFY-44 ArrayExpress array assay GSM96269.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.2.zip P-GSE4219-1 ArrayExpress GSM96269_sample_table.txt norm GSM96269_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96268 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T3_R1, Spheroid Recovery Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96268 extract 1 total RNA GSM96268 LE 1 1 GSM96268 A-AFFY-44 ArrayExpress array assay GSM96268.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.2.zip P-GSE4219-1 ArrayExpress GSM96268_sample_table.txt norm GSM96268_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96267 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T2_R3, Spheroid Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96267 extract 1 total RNA GSM96267 LE 1 1 GSM96267 A-AFFY-44 ArrayExpress array assay GSM96267.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.2.zip P-GSE4219-1 ArrayExpress GSM96267_sample_table.txt norm GSM96267_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96266 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T2_R2, Spheroid Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96266 extract 1 total RNA GSM96266 LE 1 1 GSM96266 A-AFFY-44 ArrayExpress array assay GSM96266.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.2.zip P-GSE4219-1 ArrayExpress GSM96266_sample_table.txt norm GSM96266_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96265 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T2_R1, Spheroid Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96265 extract 1 total RNA GSM96265 LE 1 1 GSM96265 A-AFFY-44 ArrayExpress array assay GSM96265.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.2.zip P-GSE4219-1 ArrayExpress GSM96265_sample_table.txt norm GSM96265_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96264 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T1_R3, Monolayer Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96264 extract 1 total RNA GSM96264 LE 1 1 GSM96264 A-AFFY-44 ArrayExpress array assay GSM96264.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.2.zip P-GSE4219-1 ArrayExpress GSM96264_sample_table.txt norm GSM96264_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96263 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T1_R2, Monolayer Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96263 extract 1 total RNA GSM96263 LE 1 1 GSM96263 A-AFFY-44 ArrayExpress array assay GSM96263.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.2.zip P-GSE4219-1 ArrayExpress GSM96263_sample_table.txt norm GSM96263_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip GSM96262 1 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample). Human Foreskin Fibroblast (HFF2) Primary Cells HFF2_T1_R1, Monolayer Homo sapiens EFO http://purl.obolibrary.org/obo/NCBITaxon_9606 GSM96262 extract 1 total RNA GSM96262 LE 1 1 GSM96262 A-AFFY-44 ArrayExpress array assay GSM96262.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.raw.2.zip P-GSE4219-1 ArrayExpress GSM96262_sample_table.txt norm GSM96262_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-4219/E-GEOD-4219.processed.1.zip