Comment[ArrayExpressAccession]	E-GEOD-42157
MAGE-TAB Version	1.1							
Public Release Date	2013-02-01
Investigation Title	Imprinting of the Y chromosome depending on parent of origin provides insight into chromatin-modulating mechanisms underlying the Y chromosome's ability to affect gene expression diversity							
Comment[Submitted Name]	Imprinting of the Y chromosome depending on parent of origin provides insight into chromatin-modulating mechanisms underlying the Y chromosome's ability to affect gene expression diversity
Experiment Description	These arrays were done in a study to probe Y-by-parent-of-origin effects on gene expression across two geographically diverse (Ohio, USA and Republic of Congo, Africa) Y-chromosome lineages (Cs or Con), and two modes of Y chromosome transmission (paternally or maternally). Males harbor one of two Y chromosomes extracted from natural populations. Y chromosomes are inherited maternally or paternally. Therefore, 4 nodes in the design matrix. Each node was competitively hybridized to each of the other three nodes in 2 dual-channel arrays, dye-swapped.							
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl						
Person Last Name	Jiang	Jiang	Hartl					
Person First Name	Pan-Pan	Pan	Daniel					
Person Mid Initials		P	L					
Person Email	pjiang@oeb.harvard.edu							
Person Affiliation	Harvard University							
Person Address	Organismic and Evolutionary Biology, Harvard University, 16 Divinity Ave c/o Hartl Lab, Cambridge, MA, USA							
Person Roles	submitter							
Protocol Name	P-GSE42157-1	P-GSE42157-6	P-GSE42157-3	P-GSE42157-8	P-GSE42157-7	P-GSE42157-2	P-GSE42157-4	P-GSE42157-5
Protocol Description	ID_REF =  VALUE = Log2 normalized ratio (Cy5/Cy3)	Genisphere 3DNA system.	24hr light, humidity controlled, males 3 days old at time of RNA extraction.	Ratios were taken from the Limma package after Loess normalization of the raw intensities data originally obtained in GenePix.	Axon scanner and GenePix.	Treatment: 2 Y-lineages (Cs or Con) and 2 modes of Y chromosome transmission (paternal or maternal) in every possible pairing.	Trizol RNA extraction.	Genisphere 3DNA system.
Protocol Type	bioassay_data_transformation	hybridization	grow	feature_extraction	image_aquisition	specified_biomaterial_action	nucleic_acid_extraction	labeling
Experimental Factor Name	LINEAGE	PARENT OF ORIGIN OF Y CHR						
Experimental Factor Type	lineage	parent of origin of y chr						
Comment[SecondaryAccession]	GSE42157							
Comment[GEOReleaseDate]	2013-02-01							
Comment[ArrayExpressSubmissionDate]	2012-11-08							
Comment[GEOLastUpdateDate]	2013-02-02							
Comment[AEExperimentType]	transcription profiling by array							
SDRF File	E-GEOD-42157.sdrf.txt