Comment[ArrayExpressAccession]	E-GEOD-41809
MAGE-TAB Version	1.1							
Public Release Date	2012-10-25
Investigation Title	Expression data from young (4 month-old) hearts from GRK2 heterozygous C57BL/6J mice and its wild type littermates							
Comment[Submitted Name]	Expression data from young (4 month-old) hearts from GRK2 heterozygous C57BL/6J mice and its wild type littermates
Experiment Description	G protein-coupled receptor kinase 2 (GRK2) has emerged as a key regulator of cardiac function and myocardial structure. Cardiac GRK2 is increased in heart failure and ischemia in humans, whereas genetic inhibition of GRK2 is cardioprotective in animal models of these pathologies. However, the mechanistic basis underlying these effects are not fully understood. We have used adult GRK2 hemizygous mice (GRK2+/-) as a model to assess the effects of a sustained systemic inhibition of GRK2 in heart tissue with age. We used microarrays to determine the global programme of gene expression underlying cardioprotection in GRK2 hemizygous mice. 4 month-old mice hearts were collected for RNA extraction and hybridization on Affymetrix microarrays.							
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl						
Person Last Name	Elisa	Jurado-Pueyo	Lucas					
Person First Name	Lucas	Maria	E					
Person Email	elucas@cbm.uam.es							
Person Affiliation	Centro de BiologM-CM--a Molecular "Severo Ochoa" (CSIC-UAM)							
Person Address	Molecular Biology, Centro de BiologM-CM--a Molecular "Severo Ochoa" (CSIC-UAM), Nicolas Cabrera 1, Madrid, Madrid, Spain							
Person Roles	submitter							
Protocol Name	P-GSE41809-1	P-GSE41809-6	P-GSE41809-3	P-GSE41809-8	P-GSE41809-7	P-GSE41809-2	P-GSE41809-4	P-GSE41809-5
Protocol Description	ID_REF =  VALUE = log2 RMA signal intensity	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChipM-. Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.	The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.	Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-HochbergM-bM-^@M-^Ys method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).	GeneChips were scanned using GeneChipM-. Scanner 3000 7G System	Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4M-:C. Then samples were stored at -70M-:C untill RNA extraction.	frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Protocol Type	bioassay_data_transformation	hybridization	grow	feature_extraction	image_aquisition	specified_biomaterial_action	nucleic_acid_extraction	labeling
Comment[SecondaryAccession]	GSE41809							
Comment[GEOReleaseDate]	2012-10-25							
Comment[ArrayExpressSubmissionDate]	2012-10-24							
Comment[GEOLastUpdateDate]	2012-10-31							
Comment[AEExperimentType]	transcription profiling by array							
SDRF File	E-GEOD-41809.sdrf.txt