Comment[ArrayExpressAccession]	E-GEOD-40983
MAGE-TAB Version	1.1						
Public Release Date	2012-09-20
Investigation Title	BMPER regulates cardiomyocyte size and vessel density in vivo						
Comment[Submitted Name]	BMPER regulates cardiomyocyte size and vessel density in vivo
Experiment Description	Background:  BMPER, an orthologue of Drosophila melanogaster crossveinless-2, is a secreted factor that regulates BMP activity in endothelial cell precursors and during early cardiomyocyte differentiation.  Although previously described in the heart, the role of Bmper in cardiac development and function remained unknown.  Methods: BMPER deficient hearts were phenotyped histologically and functionally using echocardiography and Doppler analysis.  Since BMPER -/- mice die perinatally, BMPER +/- mice were then challenged to pressure overload induced cardiac hypertrophy and hind limb ischemia to determine changes in angiogensis and regulation of cardiomyocyte size. Results: We identified for the first time the cardiac phenotype associated with BMPER haploinsufficiency.  BMPER mRNA and protein are present in the heart during cardiac development through at least E14.5 but is lost by E18.5.  BMPER +/- ventricles are thinner and less compact than sibling wild-type hearts.  In the adult, BMPER +/- hearts present with decreased anterior and posterior wall thickness, decreased cardiomyocyte size, and an increase in cardiac vessel density. Despite these changes, BMPER +/- mice respond to pressure overload-induced cardiac hypertrophy challenge largely to the same extent as wild-type mice.  Conclusion: BMPER appears to play a role in regulating both vessel density and cardiac development in vivo; however, BMPER haploinsufficiency does not result in marked effects on cardiac function or adaptation to pressure overload hypertrophy. Unpaired, two-condition experiment, wild-type vs BMPER+/- adult hearts. Biological replicates: 4 per condition.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Schisler	Schisler	Kang	Willis	Patterson		
Person First Name	Jonathan	Jonathan	Eunice	Monte	Cam		
Person Mid Initials	C	C		S			
Person Email	schisler@gmail.com						
Person Affiliation	University of North Carolina School of Medicine						
Person Phone	919-843-8708						
Person Fax	919-843-4585						
Person Address	University of North Carolina School of Medicine, MBRB, Rm 2330, Chapel Hill, NC, USA						
Person Roles	submitter						
Protocol Name	P-GSE40983-1	P-GSE40983-6	P-GSE40983-5	P-GSE40983-2	P-GSE40983-3	P-GSE40983-4	P-GSE40983-7
Protocol Description	ID_REF =  VALUE = Normalized log2 ratio (Cy5/Cy3) representing sample/reference	Scanned on an Axon Instruments GenePix 4000b scanner and processed using Agilent FeatureExtractor 9.5.1.1.	Cy-5 labeled cRNA was co-hybridized to Agilent 014868 Whole Mouse Genome Microarrays with equimolar amounts of Cy-3 labeled WMRR-derived cRNA. Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially.	Heart ventricle tissues were isolated by dissection from wild-type and BMPER+/- adult mice	Total RNA was extracted using the TRIzol method (Invitrogen), followed by an RNA cleanup step and on-column DNA digestion using the Rneasy miniprep kit according manufacturers' instructions	500 ng of WMRR or heart RNA labeled with Cy-3 or Cy-5, respectively (Perkin Elmer) and amplified using the Low Input RNA Amplification Kit (Agilent) as described by the manufacturer.	Samples were loess normalized per array. Data files were uploaded into Genespring 11.5.1 using a median value baseline transformation. Entities where at least 50 percent of the samples have flags in Comprimised or Not Detected were filtered and removed. Remaining entities with Compromised or Not Detected flags (maximum 3 samples per entity) were treated as missing and imputed using k nearest neighbors (k = 5).
Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	specified_biomaterial_action	nucleic_acid_extraction	labeling	feature_extraction
Experimental Factor Name	DEVELOPMENTAL STAGE	STRAIN OR LINE	TISSUE TYPE				
Experimental Factor Type	developmental stage	strain or line	tissue type				
Comment[SecondaryAccession]	GSE40983						
Comment[GEOReleaseDate]	2012-09-20						
Comment[ArrayExpressSubmissionDate]	2012-09-19						
Comment[GEOLastUpdateDate]	2012-09-21						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-40983.sdrf.txt