Comment[ArrayExpressAccession]	E-GEOD-37458							
MAGE-TAB Version	1.1							
Public Release Date	2012-11-14							
Investigation Title	Expression data from WT and VAChT KDHOM ventricles							
Comment[Submitted Name]	Expression data from WT and VAChT KDHOM ventricles							
Comment[AEExperimentDisplayName]	Transcription profiling by array of ventricles from mice with homozygous knockdown expression of the vesicular acetylcholine transporter (VAChT) and wild type controls							
Experiment Description	VAChT KDHOM mice have a 70% decrease in the vesicular acetylcholine transporter (VAChT) and this leads to a systemic decrease in ACh release and cardiac dysfunction. We used this microarray to determine whether there were transcriptional alterations in the hearts of mutant mice as a result of this long-term decrease in cholinergic tone. We isolated whole hearts from 3-month-old WT and VAChT KD mice and used them for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogenous populations for each sample and, as such, pooled together two RNA samples to generate one microarray sample.							
Term Source Name	ArrayExpress	EFO						
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo						
Person Last Name	Carter	Marco A.M.	Robert					
Person First Name	David	Prado	Gros					
Person Mid Initials	E							
Person Email	dcarter@robarts.ca							
Person Affiliation	Robarts Research Institute							
Person Address	London Regional Genomics Centre, Robarts Research Institute, 100 Perth Drive, London, ON, Canada							
Person Roles	submitter							
Protocol Name	P-GSE37458-1	P-GSE37458-6	P-GSE37458-3	P-GSE37458-8	P-GSE37458-7	P-GSE37458-2	P-GSE37458-4	P-GSE37458-5
Protocol Description	ID_REF =  VALUE = RMA signal intensity	5.5 ug of single stranded cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.	WT and VAChT KD mice were aged to 3 months of age before hearts were excised for RNA extraction.	The data were analyzed with Partek Genomcis Suite using the RMA normalization method	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.	No treatments were applied to the animals or tissue.	RNA was extracted using the Fatty and Fibrous Tissue RNA Extraction Kit (Bio-rad)	Biotin end labeled single strand cDNA was prepared according to the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual
Protocol Type	bioassay_data_transformation	hybridization	grow	feature_extraction	image_aquisition	specified_biomaterial_action	nucleic_acid_extraction	labeling
Experimental Factor Name	genotype							
Experimental Factor Type	genotype							
Publication Title	An analysis of the myocardial transcriptome in a mouse model of cardiac dysfunction with decreased cholinergic neurotransmission.							
Publication Author List	Roy A, Lara A, Guimar�es D, Pires R, Gomes ER, Carter DE, Gomez MV, Guatimosim S, Prado VF, Prado MA, Gros R							
PubMed ID	22768193							
Publication DOI	10.1371/journal.pone.0039997							
Comment[SecondaryAccession]	GSE37458							
Comment[GEOReleaseDate]	2012-11-14							
Comment[ArrayExpressSubmissionDate]	2012-04-20							
Comment[GEOLastUpdateDate]	2012-11-14							
Comment[AEExperimentType]	transcription profiling by array							
SDRF File	E-GEOD-37458.sdrf.txt