Comment[ArrayExpressAccession]	E-GEOD-36674
Public Release Date	2012-03-22					
Investigation Title	Expression data for mouse hypothalamus					
Comment[Submitted Name]	Expression data for mouse hypothalamus
Experiment Description	Strain differences in gene expression in the hypothalamus of BXD recombinant inbred mice We used microarrays to evaluate genetic and sex-specific differences in gene expression in the hypothalamus Hypothalamus was dissected from adult male and female mice and process for expression analysis					
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Term Source Name	EFO
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Term Source File	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Mozhui	Mozhui	Williams			
Person First Name	Khyobeni	K	R			
Person Mid Initials			W			
Person Email	geo@ncbi.nlm.nih.gov					
Person Affiliation	University of Tennessee Health Science Center					
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Person Address	Anatomy & Neurobiology, University of Tennessee Health Science Center, 855 Monroe Ave, Suite 515, Memphis, TN, USA					
Person Roles	submitter					
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Protocol Name	P-GSE36674-1	P-GSE36674-5	P-GSE36674-4	P-GSE36674-2	P-GSE36674-3	P-GSE36674-6
Protocol Description	ID_REF = <br>VALUE = RMA normalized, and z-scored on a log2 scale to a mean of 8 and standard deviation of 2	Arrays were scanned using the Affymetrix GCS 3000	The wash and staining are done on a Affymetrix Fluidics Station 450 and hybed and scanned using Affymetrix manufactures protocols for the Mouse Gene ST 1.0	RNA was purified using the RNAeasy micro kit on the QIAcube system (Qiagen, www.qiagen.com).	100ng of total RNA were amplified and labeled according to Affymetrix manufactures protocols for the Mouse Gene ST 1.0. Samples were amplified using the Ambion Whole Transcriptome Amplification Kit using manufactures protocols and the fragmentation and labeling was done according to Affymetrix manufactures protocols.	Probe intensity values were extracted using the Affymetrix GeneChip Operating Software and RMA normalized
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Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	nucleic_acid_extraction	labeling	feature_extraction
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Experimental Factor Name	AGE (DAYS)	STRAIN OR LINE	SEX			
Experimental Factor Type	age (days)	strain or line	Sex			
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Comment[SecondaryAccession]	GSE36674					
Comment[GEOLastUpdateDate]	2012-03-27
Comment[AEExperimentType]	transcription profiling by array					
Comment[GEOReleaseDate]	2012-03-22
Comment[ArrayExpressSubmissionDate]	2012-03-21
SDRF File	E-GEOD-36674.sdrf.txt