Comment[ArrayExpressAccession]	E-GEOD-36135						
MAGE-TAB Version	1.1						
Public Release Date	2012-10-02						
Investigation Title	Expression data from Docetaxel-resistant prostate cancer cells						
Comment[Submitted Name]	Expression data from prostate cancer Docetaxel-resistant cells						
Experiment Description	Docetaxel is the standard first line therapy for hormone-refractory prostate cancer patients. Here we generated models of Docetaxel resistance in prostate cancer cells to study the molecular pathways that drive the acquisition of resistance to this therapy. We used microarrays to detail the global program of gene expression underlying the acquisition of Docetaxel resistance in prostate cancer cells. Parental Docetaxel-sensitive prostate cancer cell lines (DU145 and 22Rv1) and selected Docetaxel-resistant cells (DU145-DR and 22Rv1-DR) were harvested for RNA extraction and hybridization on Affymetrix microarrays. Samples were analyzed in triplicates in order to increase the resolution of expression profiles.						
Term Source Name	ArrayExpress	EFO					
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Domingo-Domenech	Domingo-Domenech	Cordon-Cardo				
Person First Name	Josep	Josep	Carlos				
Person Email	josep.domingo-domenech@mssm.edu						
Person Affiliation	Mount Sinai School of Medicine						
Person Phone	212-241-9145						
Person Address	Pathology, Mount Sinai School of Medicine, Annenberg Building, Room 24-16, New York, New York, USA						
Person Roles	submitter						
Protocol Name	P-GSE36135-1	P-GSE36135-6	P-GSE36135-5	P-GSE36135-3	P-GSE36135-2	P-GSE36135-4	P-GSE36135-7
Protocol Description	ID_REF =  VALUE = Normalized signal intensity	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.	Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix Human U133 Plus 2.0 or Affymetrix Human U133A 2.0 arrays.	Trizol extraction of total RNA was performed according to the manufacturer's instructions.	Parental Docetaxel-sensitive and resistant cells were grown with RPMI supplemented with 10% FBS alone or under exposure to Docetaxel, respectively, before harvesting total RNA.	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.	The data were analyzed with Microarray Suite, MicroDB, and Data Mining tool software (Affymetrix). Quantile normalization and RMA summarization were used to obtain probeset level expression.
Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	nucleic_acid_extraction	grow	labeling	feature_extraction
Experimental Factor Name	cell line	phenotype					
Experimental Factor Type	cell line	phenotype					
Publication Title	Suppression of Acquired Docetaxel Resistance in Prostate Cancer through Depletion of Notch- and Hedgehog-Dependent Tumor-Initiating Cells.						
Publication Author List	Domingo-Domenech J, Vidal SJ, Rodriguez-Bravo V, Castillo-Martin M, Quinn SA, Rodriguez-Barrueco R, Bonal DM, Charytonowicz E, Gladoun N, de la Iglesia-Vicente J, Petrylak DP, Benson MC, Silva JM, Cordon-Cardo C						
PubMed ID	22975379						
Publication DOI	10.1016/j.ccr.2012.07.016						
Comment[SecondaryAccession]	GSE36135						
Comment[GEOReleaseDate]	2012-10-02						
Comment[ArrayExpressSubmissionDate]	2012-02-28						
Comment[GEOLastUpdateDate]	2012-10-12						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-36135.sdrf.txt