Comment[ArrayExpressAccession]	E-GEOD-35993
Public Release Date	2012-05-22							
Investigation Title	Notch regulated miRNAs in human T-ALL cell line							
Comment[Submitted Name]	Notch regulated miRNAs in human T-ALL cell line
Experiment Description	In an attempt to identify miRNAs regulated by oncogenic Notch signaling, we performed miRNA profiling of human T-cell acute lymphoblastic leukemia (T-ALL) cells with or without the treatment of γ-secretase inhibitor (GSI) to block Notch signaling. We found miR-223 levels to increase after GSI treatment suggesting that active Notch signaling represses miR-223 expression. We confirmed insulin-like growth factor-1 receptor (IGF1R) to be regulated by miR-223, but were unable to demonstrate functional effects on T-ALL cell growth by overexpression or knock-down of miR-223 alone. 4 samples analyzed, 2 cell lines each treated (GSI) and mock treated							
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Term Source Name	EFO
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Term Source File	http://www.ebi.ac.uk/efo/efo.owl							
Person Last Name	Weng	Gusscott	Weng					
Person First Name	Andrew	Sam	Andrew					
Person Mid Initials	P	D	P					
Person Email	aweng@bccrc.ca							
Person Affiliation	BC Cancer Agency							
Person Phone	604-675-8136							
Person Fax	604-877-0712							
Person Address	Pathology, BC Cancer Agency, 675 West 10th Ave, Vancouver, BC, Canada							
Person Roles	submitter							
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Protocol Name	P-GSE35993-1	P-GSE35993-6	P-GSE35993-3	P-GSE35993-8	P-GSE35993-7	P-GSE35993-2	P-GSE35993-4	P-GSE35993-5
Protocol Description	ID_REF = <br>VALUE = Expression is reported as log2(Hy3/Hy5) ratios	Performed as per Exiqon standard procedures.	Jurkat and P12 Ichikawa were grown under standard conditions. RPMI 1640 medium supplemented with 10% FCS, 1 mM sodium pyruvate, 2 mM L-glutamine, and antibiotics.	Performed as per Exiqon standard procedures.	Performed as per Exiqon standard procedures.	Cells were treated with the gamma-secretase inhibitor (GSI) Compound E (E) at a concentration of 1uM for 4 days followed by washout and resuspension in media containing E or Mock (DMSO).	Total RNA was extracted using standard Trizol protocol.	Individual samples were labelled with Hy3 and compared to a common reference pool of all 4 sampled labelled with Hy5.
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Protocol Type	bioassay_data_transformation	hybridization	grow	feature_extraction	image_aquisition	specified_biomaterial_action	nucleic_acid_extraction	labeling
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Experimental Factor Name	PROTOCOL	CELL LINE						
Experimental Factor Type	protocol	cell line						
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Publication Title	Notch-mediated repression of miR-223 contributes to IGF1R regulation in T-ALL.							
Publication Author List	Gusscott S, Kuchenbauer F, Humphries RK, Weng AP							
PubMed ID	22424712							
Publication DOI	10.1016/j.leukres.2012.02.013							
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Comment[SecondaryAccession]	GSE35993							
Comment[GEOLastUpdateDate]	2012-05-22
Comment[AEExperimentType]	transcription profiling by array							
Comment[GEOReleaseDate]	2012-05-21
Comment[ArrayExpressSubmissionDate]	2012-02-21
SDRF File	E-GEOD-35993.sdrf.txt