Comment[ArrayExpressAccession]	E-GEOD-35972							
Public Release Date	2012-02-22							
Investigation Title	TOV112D cells treated with NSC319726							
Comment[AEExperimentDisplayName]	Transcription profiling by array of ovarian carcinoma TOV-112D cells treated with NSC319726 to investigate the effect on p53 targets							
Comment[Submitted Name]	TOV112D cells treated with NSC319726							
Experiment Description	Rescuing the function of mutant p53 protein is an attractive cancer therapeutic strategy. Using the NCI anticancer drug screen data, we identified two compounds from the thiosemicarbazone family that manifest increased growth inhibitory activity in mutant p53 cells, particularly for the p53R175 mutant.  Mechanistic studies reveal that NSC319726 restores WT structure and function to the p53R175 mutant.  This compound kills p53R172H knock-in mice with extensive apoptosis and inhibits xenograft tumor growth in a 175-allele specific mutant p53 dependent manner.  This activity depends upon the zinc ion chelating properties of the compound as well as redox changes.  These data identify NSC319726 as a p53R175 mutant reactivator and as a lead compound for p53 targeted drug development. We utilized gene expression microarrays to examine the transcriptional activity of p53 targets in TOV112D cells after treatment with NSC319726. Triplicate cultures of TOV112D cells untreated, treated with NSC319726. Total RNA was extracted and hybridized to Affymetrix human U133 plus 2.0 microarrays.							
Term Source Name	EFO							
Term Source Version								
Term Source File	http://www.ebi.ac.uk/efo/efo.owl							
Person Last Name	Vazquez	Vazquez	Yu	Carpizo				
Person First Name	Alexei	Alexei	Xin	Darren				
Person Mid Initials								
Person Email	vazqueal@umdnj.edu							
Person Affiliation	The Cancer Institute of New Jersey							
Person Phone								
Person Fax								
Person Address	The Cancer Institute of New Jersey, 195 Little Albany St, New Brunswick, NJ, USA							
Person Roles	submitter							
Person Roles Term Source REF	EFO							
Protocol Name	P-GSE35972-1	P-GSE35972-6	P-GSE35972-3	P-GSE35972-8	P-GSE35972-7	P-GSE35972-2	P-GSE35972-4	P-GSE35972-5
Protocol Description	ID_REF = <br>VALUE = RMA normalized	Hybridization was performed according to standard protocols for Affymetrix HG-U133 plus 2.0 arrays.	TOV112D cells were cultured in DMEM + 10% FBS.	The data was analyzed with Bioconductor, RMA normalization.	Arrays were scanned using a GenePix 4000B microarray scanner.	When cell confluency reached 50-60% (1 day) the cells were treated with NSC319726 (1 µM) for 24 hours before harvested for RNA extraction.	RNA was extracted from the cells using Qiagen RNeasy kit.	Biotinylated cRNA was generated using standard Affymetrix protocols.
Protocol Type	normalization data transformation protocol	nucleic acid hybridization to array protocol	growth protocol	array scanning and feature extraction protocol	array scanning and feature extraction protocol	treatment protocol	nucleic acid extraction protocol	nucleic acid labeling protocol
Protocol Term Source REF								
Protocol Term Accession Number								
Experimental Factor Name	compound	dose						
Experimental Factor Type	compound	dose						
Experimental Factor Term Source REF								
Experimental Factor Term Accession Number								
Publication Title	Allele-specific p53 mutant reactivation.							
Publication Author List	Yu X, Vazquez A, Levine AJ, Carpizo DR.							
PubMed ID	22624712							
Publication DOI	10.1016/j.ccr.2012.03.042							
Publication Status	published							
Publication Status Term Source REF								
Publication Status Term Accession Number								
Comment[SecondaryAccession]	GSE35972							
Comment[GEOLastUpdateDate]	2012-02-23							
Comment[AEExperimentType]	transcription profiling by array							
Comment[GEOReleaseDate]	2012-02-22							
Comment[ArrayExpressSubmissionDate]	2012-02-21							
SDRF File	E-GEOD-35972.sdrf.txt