Comment[ArrayExpressAccession]	E-GEOD-34299
MAGE-TAB Version	1.1							
Public Release Date	2012-09-15
Investigation Title	Expression of BRAF inhibitor resistant colon cancer lines							
Comment[Submitted Name]	Expression of BRAF inhibitor resistant colon cancer lines
Experiment Description	Colon cancer cell lines with partial sensitivity to the BRAF inhibitor PLX4720 were grown in increasing concentration of the drug to develop acquired resistance.  Gene expression was performed for comparison of the resistant clones to the parental lines. Colon cancer cell lines with partial sensitivity to the BRAF inhibitor PLX4720 were grown in increasing concentration of the drug to develop acquired resistance.  Gene expression was performed for comparison of the resistant clones to the parental lines.							
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl						
Person Last Name	Kopetz	Kopetz						
Person First Name	Scott	Scott						
Person Email	skopetz@mdanderson.org							
Person Affiliation	MD Anderson Cancer Center							
Person Phone	713-792-2828							
Person Address	MD Anderson Cancer Center, 1515 Holcombe Blvd, Unit 426, Houston, TX, USA							
Person Roles	submitter							
Protocol Name	P-GSE34299-1	P-GSE34299-6	P-GSE34299-3	P-GSE34299-8	P-GSE34299-7	P-GSE34299-2	P-GSE34299-4	P-GSE34299-5
Protocol Description	ID_REF =  VALUE = log2 GC-RMA	hybridization to Affymetrix HGU133 plus2 according to the standard Affymetrix protocol	Culture dish + Media with 10%serum	The data were first quantified using the MAS5 algorithm. The Robust Multiarray Analysis (RMA) algorithm was used to normalize and quantify the data.	imaging on GeneChip Scanner 3000 (Affymetrix)	Resistant lines were grown in PLX4720-free media x 24 hours prior to harvesting	Total RNA was extracted cell lines using the Qiagen RNeasy Kit according to the manufacturer's protocol.	Biotinylated cRNA were prepared according to the standard Affymetrix protocol
Protocol Type	bioassay_data_transformation	hybridization	grow	feature_extraction	image_aquisition	specified_biomaterial_action	nucleic_acid_extraction	labeling
Experimental Factor Name	CELL LINE							
Experimental Factor Type	cell line							
Comment[SecondaryAccession]	GSE34299							
Comment[GEOReleaseDate]	2012-09-15							
Comment[ArrayExpressSubmissionDate]	2011-12-09							
Comment[GEOLastUpdateDate]	2012-09-18							
Comment[AEExperimentType]	transcription profiling by array							
SDRF File	E-GEOD-34299.sdrf.txt