Comment[ArrayExpressAccession]	E-GEOD-34066
MAGE-TAB Version	1.1												
Public Release Date	2013-01-01
Investigation Title	Expression data from mesodermal Tbx1-knockout ears, otic vesicles, and periotic mesenchyme												
Comment[Submitted Name]	Expression data from mesodermal Tbx1-knockout ears, otic vesicles, and periotic mesenchyme
Experiment Description	This SuperSeries is composed of the SubSeries listed below. Refer to individual Series												
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl											
Person Last Name	Guo												
Person First Name	Tingwei												
Person Email	tingwei.guo@einstein.yu.edu												
Person Affiliation	Albert Einstein College of Medicine												
Person Phone	(718) 678-1122												
Person Address	Genetics, Albert Einstein College of Medicine, 1301 Morris Park Avenue, Room 408, Bronx, NY, USA												
Person Roles	submitter												
Protocol Name	P-GSE34066-2	P-GSE34066-1	P-GSE34066-11	P-GSE34066-6	P-GSE34066-3	P-GSE34066-13	P-GSE34066-10	P-GSE34066-12	P-GSE34066-8	P-GSE34066-7	P-GSE34066-9	P-GSE34066-4	P-GSE34066-5
Protocol Description	ID_REF =  VALUE = MAS5.0 signal intensity ABS_CALL = indicating whether the transcript was present (P), absent (A), or marginal (M) DETECTION P-VALUE = 	ID_REF =  VALUE = RMA signal intensity	10ug of cRNA was hybridized for 16hrs at 45C on Affymetrix Mouse 430_2 arrays.	10ug of cRNA was hybridized for 16hrs at 45C on Affymetrix Mouse Gene ST 1.0 arrays.	Timed matings were dissected at embryonic day 12.5, and periotic tissue was isolated and immediately processed for RNA isolation. Embryos were dissected and microdissected in cold PBS.	Timed matings were dissected at embryonic day 11.5, and periotic tissue was isolated, including otic vesicle and perotic mesenchyme. RNA was isolated from the tissue immediately following dissection.	Timed matings were dissected at embryonic day 11.5, and periotic tissue was isolated.	Data was analyzed using Affymetrix Expression Console software version 1.1 using Affymetrix default protocols.	Data was analyzed by RMA using Affymetrix Expression Console software version 1.1 using Affymetrix default protocols.	Arrays were scanned using the Affymetrix GeneChip 7G Scanner.	Periotic tissue was incubated with dispase for 30 min at 37C to remove periotic tissue, folllowed by RNA isolation.	Qiagen RNeasy Micro kit according to the manufacturer's protocol.	Biotinylated cRNA was prepared according to Affymetrix protocols from 300ng of total RNA.
Protocol Type	bioassay_data_transformation	bioassay_data_transformation	hybridization	hybridization	grow	grow	grow	feature_extraction	feature_extraction	image_aquisition	specified_biomaterial_action	nucleic_acid_extraction	labeling
Experimental Factor Name	GENOTYPE	ORGANISM PART	AGE										
Experimental Factor Type	genotype	organism part	age										
Comment[SecondaryAccession]	GSE34066												
Comment[GEOReleaseDate]	2013-01-01												
Comment[ArrayExpressSubmissionDate]	2011-11-30												
Comment[GEOLastUpdateDate]	2013-01-02												
Comment[AEExperimentType]	transcription profiling by array												
SDRF File	E-GEOD-34066.sdrf.txt