Comment[ArrayExpressAccession]	E-GEOD-34030
MAGE-TAB Version	1.1						
Public Release Date	2011-11-30
Investigation Title	LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function [RNA-Seq]						
Comment[Submitted Name]	LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function [RNA-Seq]
Experiment Description	Two separate pools of three Flox or two Lrh1t-/- pancreas RNA preparations was subjected to the Illumina RNA-seq protocol. Examination of mRNA levels in induced Lrh1 pancreas knockout and wild-type mice.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Ochsner	Holmstrom	Deering	Swift	Kliewer	MacDonald	Mangelsdorf
Person First Name	Scott	Sam	Tye	Galvin	Steven	Raymond	David
Person Mid Initials	Andrew	R			A		J
Person Email	sochsner@bcm.edu						
Person Affiliation	Baylor College of Medicine						
Person Phone	713-798-6227						
Person Address	Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, USA						
Person Roles	submitter						
Protocol Name	P-GSE34030-2	P-GSE34030-1	P-GSE34030-3				
Protocol Description	Ten micrograms of total RNA from two separate pools of three Flox or two LRHt-/- pancreas RNA preparations was subjected to the Illumina RNA-seq protocol (Illumina). DNA (0.7 fmol) from each amplified library was analyzed with an Illumina Genome Analyzer.	To generate the tamoxifen-inducible cre Lrh1 deletion, mice with floxed Lrh1 alleles (Lee et al. 2008) were crossed with a ROSA26 Tam-ERCre strain (no. 004847, Jackson Laboratories). This line was backcrossed to generate a mixed strain, homozygous Lrh1 floxed, and heterozygous ROSA26 Tam-ERCre line. From this line, adult male mice with or without ROSA26 Tam-ERCre (3–5 mo old) were treated with 0.25 mg/g tamoxifen (T5648, Sigma-Aldrich) in corn oil by oral gavage for two consecutive days followed by sacrifice after 5 d. Mice were kept on a 12-h light–dark cycle from 6:00 am to 6:00 pm with free access to water and standard chow diet (TD.2016, Harland-Teklad Laboratories).	The sequence tags were aligned to the mouse (mm9) genome with Bowtie 3.				
Protocol Type	nucleic acid library construction protocol	grow	feature_extraction				
Experimental Factor Name	GENOTYPE						
Experimental Factor Type	genotype						
Publication Title	LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function.						
Publication Author List	Holmstrom SR, Deering T, Swift GH, Poelwijk FJ, Mangelsdorf DJ, Kliewer SA, MacDonald RJ						
PubMed ID	21852532						
Publication DOI	10.1101/gad.16860911						
Comment[SecondaryAccession]	GSE34030						
Comment[GEOReleaseDate]	2011-11-30						
Comment[ArrayExpressSubmissionDate]	2011-11-29						
Comment[GEOLastUpdateDate]	2013-02-26						
Comment[AEExperimentType]	RNA-seq of coding RNA						
Comment[SecondaryAccession]	SRP009598						
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/SRR387732-SRR387735						
SDRF File	E-GEOD-34030.sdrf.txt