Comment[ArrayExpressAccession]	E-GEOD-31447
Public Release Date	2011-08-22						
Investigation Title	Effect of endoplasmic reticulum stress on gene expression in lymphoblastoid cell lines.						
Comment[Submitted Name]	Effect of endoplasmic reticulum stress on gene expression in lymphoblastoid cell lines.
Experiment Description	The ER stress inducing agent Thapsigargin (TG) and/or the cytoprotective agent Salubrinal were applied to lymphoblastoid cell lines. TG induced lytic replication as well as a distinct pattern of gene expression changes. This study was designed to identify host genes mediating lytic replication secondary to ER stress. DMSO or TG applied for 6 hrs then washed out, immediately followed by either (i) DMSO, or  (ii) SAL resulting in 4 conditions (DMSO, TG, SAL, or TG+SAL). Total RNA harvested at 3, 24 or 72hrs for Affymetrix analysis.						
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Term Source Name	EFO
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Term Source File	http://www.ebi.ac.uk/efo/efo.owl						
Person Last Name	Rosendorff	Rosendorff					
Person First Name	Adam	Adam					
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Person Email	arosendorff@gmail.com						
Person Affiliation	University of Pittsburgh						
Person Phone	412-692-9015						
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Person Address	Pathology, University of Pittsburgh, 530 45th Street, 3rd Fl, Bay 9, Pittsburgh, PA, USA						
Person Roles	submitter						
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Protocol Name	P-GSE31447-1	P-GSE31447-5	P-GSE31447-4	P-GSE31447-2	P-GSE31447-7	P-GSE31447-3	P-GSE31447-6
Protocol Description	ID_REF = <br>VALUE = RMA signal	Standard Affymetrix Protocol	Standard Affymetrix Protocol	Trizol	Standard Affymetrix	Standard Affymetrix Protocol	RMA, Expression Console
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Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	nucleic_acid_extraction	nucleic_acid_extraction	labeling	feature_extraction
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Experimental Factor Name	TREATMENT	TIME					
Experimental Factor Type	treatment	time					
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Publication Title	ER-stress causes Epstein-Barr virus lytic replication.						
Publication Author List	Taylor GM, Raghuwanshi SK, Rowe DT, Wadowsky R, Rosendorff A						
PubMed ID	21849482						
Publication DOI	10.1182/blood-2011-04-347112						
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Comment[SecondaryAccession]	GSE31447						
Comment[GEOLastUpdateDate]	2011-08-22
Comment[AEExperimentType]	transcription profiling by array						
Comment[GEOReleaseDate]	2011-08-21
Comment[ArrayExpressSubmissionDate]	2011-08-16
SDRF File	E-GEOD-31447.sdrf.txt