Comment[ArrayExpressAccession]	E-GEOD-31305
MAGE-TAB Version	1.1						
Public Release Date	2013-07-17
Investigation Title	Expression data of myeloma CD138+ and CD138- populations						
Comment[Submitted Name]	Expression data of myeloma CD138+ and CD138- populations
Experiment Description	Comparison of gene expression profiles between CD138+ and CD138- populations from human myeloma cell lines RPMI-8226 and NCI-H929. We used Affymetrix human gene 1.0ST array and analyzed with GeneSpring GX. CD138+ and CD138- subsets of cells were separated using FACS and clonogenecity of both population were compared. The RNA was extracted and hybridized with Affymetrix Human Gene 1.0ST array.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Reghunathan	Reghunathan	Chonglei				
Person First Name	Renji	Renji	Bi				
Person Email	Renji_REGHUNATHAN@nuhs.edu.sg						
Person Affiliation	National University Hospital						
Person Address	National University Hospital, KentRidge Road, Singapore, Singapore						
Person Roles	submitter						
Protocol Name	P-GSE31305-1	P-GSE31305-4	P-GSE31305-5	P-GSE31305-2	P-GSE31305-7	P-GSE31305-3	P-GSE31305-6
Protocol Description	The data were analyzed with Genespring GX. ID_REF =  VALUE = RMA	200ng of total RNA were amplified and labeled using Affymetrix Whole Transcript Sense Target Labeling and control reagents kit (Affymetrix).	Biotinylated samples were hybridized onto the array for 16hrs and 60rpm in a GeneChip Hybridization Oven 640 (Affymetrix).	Cells were grown in RPMI-1640 complete medium (10% FBS+1% Penicillin and Streptomycin+1%L-Glutamine), in 37°C with 5% CO2.	Cells were grown in RPMI-1640 complete medium (10% FBS+1% Penicilliin and Streptomycin+1%L-Glutamine), in 37°C with 5% CO2.	Total RNA was extracted using RNeasy plus mini kit (Qiagen).	GeneChips were scanned using the affymetrix scanner 3000 7G.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	growth protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	CELL LINE	CELL TYPE					
Experimental Factor Type	cell line	cell type					
Comment[SecondaryAccession]	GSE31305						
Comment[GEOReleaseDate]	2013-07-17						
Comment[ArrayExpressSubmissionDate]	2011-08-10						
Comment[GEOLastUpdateDate]	2013-07-18						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-31305.sdrf.txt