Comment[ArrayExpressAccession]	E-GEOD-28737
MAGE-TAB Version	1.1					
Public Release Date	2012-09-21
Investigation Title	Conditional deletion of Bcl6 in naive B cells					
Comment[Submitted Name]	Conditional deletion of Bcl6 in naive B cells
Experiment Description	Bcl6 germline deletion causes a prominent inflammatory disease, owing to over-expression of Th2 cytokines, and affects the properties of B cells prior to immunization. Therefore we established the B cell-specific Bcl6 deletion mice and analyze the gene expression of naive B cells under physiological conditions. Total RNAs of splenic follicular and marginal zone B cells from Bcl6(flox/flox), Bcl6(+/flox) or Bcl6(+/+) mice heterozygous for mb1-cre were extracted and analysed on Affymetrix microarrays.					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Tomohiro	Sugimoto	Hikida	Hijikata	Takemori	
Person First Name	Kaji	Akiko	Masaki	Atsushi	Toshitada	
Person Email	t-kaji@rcai.riken.jp					
Person Affiliation	RIKEN					
Person Phone	81-45-503-9105					
Person Fax	81-45-503-7053					
Person Address	Research center for allergy and immunology, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, Japan					
Person Roles	submitter					
Protocol Name	P-GSE28737-1	P-GSE28737-5	P-GSE28737-4	P-GSE28737-2	P-GSE28737-3	P-GSE28737-6
Protocol Description	ID_REF =  VALUE = log2 GC-RMA	Using a microarray scanner as manufacture's instruction (Affymetrix)	Affymetrix standard procedures	Total RNAs were extracted using TRIZOL reagent.	GeneChip 3'IVT Express Kit	Follicular B cells and Marginal zone B cells were separately processed with GCRMA.
Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	nucleic_acid_extraction	labeling	feature_extraction
Experimental Factor Name	GENOTYPE					
Experimental Factor Type	genotype					
Comment[SecondaryAccession]	GSE28737					
Comment[GEOReleaseDate]	2012-09-21					
Comment[ArrayExpressSubmissionDate]	2011-04-20					
Comment[GEOLastUpdateDate]	2012-09-22					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-28737.sdrf.txt