Comment[ArrayExpressAccession]	E-GEOD-24624
Public Release Date	2010-12-07
Investigation Title	Understanding the Diversity of Maize Cell Wall (affy_cellwall_maize)																
Comment[Submitted Name]	Understanding the Diversity of Maize Cell Wall (affy_cellwall_maize)
Experiment Description	affy_cellwall_maize - affy_cellwall_maize - Maize, Zea mays, is one of the most widely grown crops in the world as it provides cereal grain for human purposes and feedstock for cattle. Maize is increasingly used as a biomass fuel, such as ethanol. Studies have shown that maize stem is made up of different lignified cell types: epidermis, sclerenchyma (a few layers under the epidermis and surrounding vascular bundles in the rind), parenchyma (mostly lignified near the epidermis). One of the aims of this work is to find out if it is the proportion of these different cell types or their number (especially for vascular bundles) which influences the degree of digestibility of maize lines. For that the objective of our work is to develop a genomic strategic approach allowing an improvement of traits involved in cell wall degradability. The work will include the mapping of genes considered as strategic in the cell wall biosynthesis, the search of new genes of interest through bio-analysis, the functional analysis of genes suspected to be of importance in cell wall pathway, the search of candidate underlying QTL, and transcriptomic approaches in order to find genes co-regulation, transcription.-Comparison of internodes from 5 maize lines grown in field conditions 10 arrays - maize; organ comparison																
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Term Source Name	EFO
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Term Source File	http://efo.svn.sourceforge.net/viewvc/efo/trunk/src/efoinowl/efo.owl																
Person Last Name	huguet	Pichon	Huguet	Pichon	Balzergue	Martin-Magniette											
Person First Name	stephanie	Magalie	Stephanie	Magalie	Sandrine	Marie-Laure											
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Person Email	huguet@evry.inra.fr																
Person Affiliation	inra																
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Person Address	inra, 2 rue gaston cremieux, evry, France																
Person Roles	submitter																
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Protocol Name	P-GSE24624-1	P-GSE24624-6	P-GSE24624-3	P-GSE24624-8	P-GSE24624-7	P-GSE24624-2	P-GSE24624-17	P-GSE24624-12	P-GSE24624-9	P-GSE24624-16	P-GSE24624-10	P-GSE24624-11	P-GSE24624-14	P-GSE24624-13	P-GSE24624-15	P-GSE24624-4	P-GSE24624-5
Protocol Description	ID_REF = ID number<br>VALUE = Normalized data by GCRMA	labelled extract quantity: 10ug	stem - Planting date: 21/04/2009 Pool de 5 plantes/ lignée/repetition conditions météo en Juillet pendant les prélèvements (station météo des Verrines) RG: Rayonnement global horaire (Joules/cm2) 202     T: Température instantanée horaraire (degrés Celsuis)20.27     U: humidité instantanée horaire (poucentage) 53	The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).	GCOS, Biotin:pmt voltage 570nm,650V,laser power 1	no treatment	ge5bl2:20ug.	ge5bl1:90ug.	ge2bl1:60ug.	ge4bl2:20ug.	ge3bl1:70ug.	ge4bl1:80ug.	ge2bl2:100ug.	ge1bl2:60ug.	ge3bl2:20ug.	ge1bl1:60ug.	labelling Biotin direct, amplification=yes, cRNA 20 ug.
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Protocol Type	bioassay_data_transformation	hybridization	grow	feature_extraction	image_aquisition	specified_biomaterial_action	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	labeling
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Experimental Factor Name	PHENOTYPE	GENOTYPE															
Experimental Factor Type	phenotype	genotype															
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Comment[SecondaryAccession]	GSE24624																
Comment[GEOLastUpdateDate]	2010-12-06
Comment[AEExperimentType]	transcription profiling by array																
Comment[GEOReleaseDate]	2010-12-07
Comment[ArrayExpressSubmissionDate]	2010-10-11
SDRF File	E-GEOD-24624.sdrf.txt