Comment[ArrayExpressAccession]	E-GEOD-24051
Public Release Date	2011-12-31							
Investigation Title	Neurological defects in CAIX knockout mice							
Comment[Submitted Name]	Neurological defects in CAIX knockout mice
Experiment Description	To further identify transcriptomic responses to CAIX deficiency in the brain, genome-wide cDNA microarray analyses were performed. Thirty-one and 37 genes were up- and down-regulated in the brain of Car9-/- mice as compared to wild-type mice. Functional annotation revealed that genes with increased expression are mainly involved in alternative splicing, nucleotide binding, RNA binding, and regulation of cellular protein metabolic process; whereas genes with reduced expression are chiefly implicated in cellular cation homeostasis, positive regulation of ion transport, Ubl conjugation, phosphorus metabolic process, and regulation of cell proliferation. Notably, the biological processes behaviour and locomotory behaviour are two prominent over-representation terms among the down-regulated genes, which is consistent with the results obtained from behavioural tests. Brain tissue samples were collected from five wild-type and four CAIX KO mice, respectively, at the age of eight months. Total RNAs were purified and used for cDNA microarray.							
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Term Source File	http://www.ebi.ac.uk/efo/efo.owl							
Person Last Name	Pan	Pan	Parkkila	Järventausta	Hilvo	Pastorekova	Pastorek	Parkkila
Person First Name	Pei-wen	Peiwen	Anna-Kaisa	Salla	Mika	Silvia	Jaromir	Seppo
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Person Email	geo@ncbi.nlm.nih.gov							
Person Affiliation	Institute of Medical Technology, University of Tampere							
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Person Address	Institute of Medical Technology, University of Tampere, Biokatu 6 , Tampere, Finland							
Person Roles	submitter							
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Protocol Name	P-GSE24051-1	P-GSE24051-5	P-GSE24051-4	P-GSE24051-2	P-GSE24051-3	P-GSE24051-6		
Protocol Description	ID_REF = <br>VALUE = quantiled normalized signal<br>Detection Pval = 	Chips were scanned with Illumina BeadArray Reader and the numerical results were extracted with Bead Studio v1.5.1.34	1.50 ug each sample was hybridized to Illumina’s SentrixÒ Mouse-6 Expression BeadChips (cat no BD-26-101) at 55 °C overnight (19 h) according to Illumina BeadStation 500X –protocol, Revision F.	Total RNAs were purified using the RNeasy Mini Kit (Qiagen, Basel, Switzerland). On-column DNase digestion was performed during RNA purification process. RNA concentrations, A260/280, and A260/230 ratios were determined using the ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA).	Using Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion), following the manufacturer's protocol. Input amount: 200ng of total RNA. Incubation of labeling reaction: 14h.	Bead Studio v1.5.1.34		
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Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	nucleic_acid_extraction	labeling	feature_extraction		
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Experimental Factor Name	GENOTYPE							
Experimental Factor Type	genotype							
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Comment[SecondaryAccession]	GSE24051							
Comment[GEOLastUpdateDate]	2011-12-31
Comment[AEExperimentType]	transcription profiling by array							
Comment[GEOReleaseDate]	2011-12-31
Comment[ArrayExpressSubmissionDate]	2010-09-08
SDRF File	E-GEOD-24051.sdrf.txt