Investigation Title Transcription profiling of mouse adipose, dermis and neural embryonic cells Comment[Submitted Name] Adipose, dermis and neural embryonic stem cells Experimental Design unknown_experiment_design_type transcription profiling by array Experimental Design Term Source REF EFO Comment[AEMIAMESCORE] 4 Comment[ArrayExpressReleaseDate] 2008-06-12 Comment[SecondaryAccession] GSE2353 Comment[ArrayExpressAccession] E-GEOD-2353 Comment[MAGETAB TimeStamp_Version] 2011-01-22 10:46:37 Last Changed Rev: 14857 Experimental Factor Name Experimental Factor Type Experimental Factor Term Source REF Person Last Name (OGIC) Person First Name OGIC Info Ontario Genomics Innovation Centre Person Mid Initials Person Email ogicinfo@ohri.ca Person Phone Person Fax Person Address Cellular and Molecular Medicine, Bioinformatics, Ottawa Health Research Institute, Ontario Genomics Innovation Centre (OGIC), 501 Smyth Rd., Ottawa, K1H 8L6, Canada Person Affiliation Ontario Genomics Innovation Centre (OGIC) Person Roles submitter Person Roles Term Source REF Quality Control Type Quality Control Term Source REF Replicate Type Replicate Term Source REF Normalization Type Normalization Term Source REF Date of Experiment Public Release Date 2008-06-12 PubMed ID Publication DOI Publication Author List Publication Title Publication Status Publication Status Term Source REF Experiment Description The objective is to study gene expression between potential adult stem cells isolated from dermis and adipose tissue compared to embryonic neural stem cells. Tissue was enzymatically digested and cells were seeded into a tissue culture flask. After 4 days, cells that had grown in suspension were passaged, harvested (by sedimentation, mechanically triturated and replated. Seven days later, cells were passaged again and RNA isolated after another 7 days. Protocol Name Affymetrix:Protocol:Hybridization-EukGE-WS2v4 P-AFFY-6 Protocol Type hybridization feature_extraction Protocol Description Title: Fluidics Station Protocol. Description: Title: Affymetrix CEL analysis. Description: Protocol Parameters Protocol Hardware Protocol Software MicroArraySuite 5.0 MicroArraySuite 5.0 Protocol Contact Protocol Term Source REF SDRF File E-GEOD-2353.sdrf.txt Term Source Name The MGED Ontology ArrayExpress EFO Term Source File http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/arrayexpress http://www.ebi.ac.uk/efo/ Term Source Version