Investigation Title	Transcription profiling of time series of C.elegans mutants and wild type	
Comment[Submitted Name]	C. elegans embryonic timecourse in wt and mutant embryos	
Experimental Design	time_series_design	individual_genetic_characteristics_design	co-expression_design	transcription profiling by array	
Experimental Design Term Source REF	mo		mo	EFO	
Comment[ArrayExpressReleaseDate]	2007-07-31	
Comment[SecondaryAccession]	GSE2180	
Comment[AEMIAMESCORE]	5	
Comment[SecondaryAccession]	GDS1319	
Comment[ArrayExpressAccession]	E-GEOD-2180	
Comment[MAGETAB TimeStamp_Version]	2010-08-04 08:27:31 Last Changed Rev: 13058	
Experimental Factor Name	Time	genotype	
Experimental Factor Type	time	genotype	
Experimental Factor Term Source REF	
Person Last Name	Hill	
Person First Name	Andrew	
Person Mid Initials	
Person Email	ahill@wyeth.com	
Person Phone	
Person Fax	
Person Address	Bioinformatics,Wyeth Research,35 CambridgePark Drive,Cambridge,02140,USA	
Person Affiliation	Wyeth Research	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2007-07-31	
PubMed ID	15772128	
Publication DOI	15772128	
Publication Author List	L Ryan Baugh,Andrew A Hill,Julia M Claggett,Kate Hill-Harfe,Joanne C Wen,Donna K Slonim,Eugene L Brown,Craig P Hunter	
Publication Title	The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	This series of samples comprises multiple early embryonic time courses for C. elegans. Time courses consisting of 10 time points each for 4 different genotypes are included: wild-type (strain N2 grown on E. coli strain OP50), pie-1(zu154) (progeny of homozygous mutant mothers [Unc] of strain JJ532 grown on E. coli strain OP50), pie-1(zu154);pal-1(RNAi) (progeny of homozygous mutant mothers [Unc] of strain JJ532 grown on E. coli strain HT115 expressing pal-1 hairpin RNA), and mex-3(zu155);skn-1(RNAi) (progeny of homozygous mutant mothers [Dpy] of strain JJ518 grown on E. coli strain HT115 expressing skn-1 hairpin RNA). Embryos were manually staged by morphology at the 4-cell stage and allowed to develop in water for defined amounts of time at 22 degrees C. RNA was amplified as described (Baugh et al. Development, 2003; Baugh et al. Nucleic Acids Research, 2001). This series of samples comprises all replicate data reported by Baugh et al. (Development, 2005).	
Protocol Name	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6	
Protocol Type	hybridization	feature_extraction	
Protocol Description	Title: Affymetrix Generic Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software	MicroArraySuite 5.0	MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF	mo	
SDRF File	E-GEOD-2180.sdrf.txt	
Term Source Name	ncbitax	mo		The MGED Ontology	ArrayExpress	mo	EFO	The MGED Ontology	
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html	http://mged.sourceforge.net/ontologies/MGEDontology.php		http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version