Source Name Characteristics [Organism] Term Source REF Term Accession Number Characteristics [GeneticBackground] Characteristics [OrganismPart] Characteristics [DevelopmentalStage] Characteristics [genetic variation] Comment [Sample_description] Comment [Sample_source_name] Protocol REF Protocol REF Extract Name Material Type Protocol REF Labeled Extract Name Label Material Type Protocol REF Protocol REF Hybridization Name Array Design REF Protocol REF Scan Name Array Data File Comment [ArrayExpress FTP file] Protocol REF Derived Array Data Matrix File Comment [Derived ArrayExpress FTP file] Factor Value [COMPOUND] Term Source REF Term Accession Number Factor Value [TREATMENT TIME] Unit[TimeUnit] GSM507117 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, estradiol, 12h P-GSE20231-3 P-GSE20231-2 GSM507117 extract 1 total_RNA P-GSE20231-4 GSM507117 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507117 A-AFFY-2 P-GSE20231-7 GSM507117.CEL GSM507117.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip 17-beta-estradiol EFO http://www.ebi.ac.uk/chebi/searchId.do;?chebiId=CHEBI:16469 12 hours GSM507120 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, zeatin, 12h P-GSE20231-3 P-GSE20231-2 GSM507120 extract 1 total_RNA P-GSE20231-4 GSM507120 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507120 A-AFFY-2 P-GSE20231-7 GSM507120.CEL GSM507120.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip trans-zeatin 12 hours GSM507121 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, zeatin, 12h P-GSE20231-3 P-GSE20231-2 GSM507121 extract 1 total_RNA P-GSE20231-4 GSM507121 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507121 A-AFFY-2 P-GSE20231-7 GSM507121.CEL GSM507121.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip trans-zeatin 12 hours GSM507124 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, estradiol, 24h P-GSE20231-3 P-GSE20231-2 GSM507124 extract 1 total_RNA P-GSE20231-4 GSM507124 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507124 A-AFFY-2 P-GSE20231-7 GSM507124.CEL GSM507124.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip 17-beta-estradiol EFO http://www.ebi.ac.uk/chebi/searchId.do;?chebiId=CHEBI:16469 24 hours GSM507119 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, estradiol, 12h P-GSE20231-3 P-GSE20231-2 GSM507119 extract 1 total_RNA P-GSE20231-4 GSM507119 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507119 A-AFFY-2 P-GSE20231-7 GSM507119.CEL GSM507119.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip 17-beta-estradiol EFO http://www.ebi.ac.uk/chebi/searchId.do;?chebiId=CHEBI:16469 12 hours GSM507115 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, control, 12h P-GSE20231-3 P-GSE20231-2 GSM507115 extract 1 total_RNA P-GSE20231-4 GSM507115 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507115 A-AFFY-2 P-GSE20231-7 GSM507115.CEL GSM507115.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip none 12 hours GSM507122 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, control, 24h P-GSE20231-3 P-GSE20231-2 GSM507122 extract 1 total_RNA P-GSE20231-4 GSM507122 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507122 A-AFFY-2 P-GSE20231-7 GSM507122.CEL GSM507122.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip none 24 hours GSM507126 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, zeatin, 24h P-GSE20231-3 P-GSE20231-2 GSM507126 extract 1 total_RNA P-GSE20231-4 GSM507126 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507126 A-AFFY-2 P-GSE20231-7 GSM507126.CEL GSM507126.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip trans-zeatin 24 hours GSM507116 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, control, 12h P-GSE20231-3 P-GSE20231-2 GSM507116 extract 1 total_RNA P-GSE20231-4 GSM507116 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507116 A-AFFY-2 P-GSE20231-7 GSM507116.CEL GSM507116.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip none 12 hours GSM507123 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, control, 24h P-GSE20231-3 P-GSE20231-2 GSM507123 extract 1 total_RNA P-GSE20231-4 GSM507123 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507123 A-AFFY-2 P-GSE20231-7 GSM507123.CEL GSM507123.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip none 24 hours GSM507118 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, estradiol, 12h P-GSE20231-3 P-GSE20231-2 GSM507118 extract 1 total_RNA P-GSE20231-4 GSM507118 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507118 A-AFFY-2 P-GSE20231-7 GSM507118.CEL GSM507118.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip 17-beta-estradiol EFO http://www.ebi.ac.uk/chebi/searchId.do;?chebiId=CHEBI:16469 12 hours GSM507127 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, zeatin, 24h P-GSE20231-3 P-GSE20231-2 GSM507127 extract 1 total_RNA P-GSE20231-4 GSM507127 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507127 A-AFFY-2 P-GSE20231-7 GSM507127.CEL GSM507127.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip trans-zeatin 24 hours GSM507125 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, estradiol, 24h P-GSE20231-3 P-GSE20231-2 GSM507125 extract 1 total_RNA P-GSE20231-4 GSM507125 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507125 A-AFFY-2 P-GSE20231-7 GSM507125.CEL GSM507125.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip 17-beta-estradiol EFO http://www.ebi.ac.uk/chebi/searchId.do;?chebiId=CHEBI:16469 24 hours GSM507114 1 Arabidopsis thaliana EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_3702 Wassilewskija primary root tip (5 mm) 1.02 T-DNA knock out mutation in IPT8 Sterilised seeds were put on agar plates and kept in +4C and darkness for 3 days. Seeds were treated with 70 % ethanol + 0.1 % Tween-20 for two minutes. They were then washed briefly with 95 % ethanol and dried. Sterilised seeds were put in rows (20-25 seeds/row) on square (12 x 12 cm) agar plates. After stratification, the plates were put in vertical position under light (LD growth chamber) and kept for 7 days. The seedlings were then treated with liquid medium for 12 or 24 h directly on the plates, and root tips from the treated seedlings were harvested for RNA extraction. 1 Agar, nutrients and sucrose were mixed with water, and pH was adjusted to 5.7. The medium was then autoclaved and poured into square petri dishes. 50/plate 23 C average, 23C day, 23C night 100 % average, 100 % day, 100 % night Murashige & Skoog basal salt mixture. Modifications: 1 % sucrose was added and pH was adjusted to 5.7. AtIPT8/pga22, control, 12h P-GSE20231-3 P-GSE20231-2 GSM507114 extract 1 total_RNA P-GSE20231-4 GSM507114 LE 1 biotin unknown P-GSE20231-5 P-GSE20231-6 GSM507114 A-AFFY-2 P-GSE20231-7 GSM507114.CEL GSM507114.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.raw.1.zip P-GSE20231-1 E-GEOD-20231-processed-data-2481656565.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-20231/E-GEOD-20231.processed.1.zip none 12 hours