Investigation Title	Transcription profiling of bone marrow samples from human acute myeloid leukemia patients							
Comment[Submitted Name]	Gene expression profile of acute myeloid leukemia							
Experimental Design	disease_state_design	co-expression_design	transcription profiling by array					
Experimental Design Term Source REF	mo	mo	EFO					
Comment[AEExperimentType]	transcription profiling by array							
Comment[AEExperimentDisplayName]	Transcription profiling by array of human bone marrow samples from acute myeloid leukemia patients							
Comment[ArrayExpressReleaseDate]	2007-08-14							
Comment[SecondaryAccession]	GSE1729							
Comment[AEMIAMESCORE]	5							
Comment[SecondaryAccession]	GDS1064							
Comment[ArrayExpressAccession]	E-GEOD-1729							
Comment[MAGETAB TimeStamp_Version]	2010-08-03 12:06:25 Last Changed Rev: 13058							
Experimental Factor Name	DISEASE STAGING	CHROMOSOMAL ABERRATION	DISEASE STATE					
Experimental Factor Type	disease_staging	chromosomal_aberration	disease_state					
Experimental Factor Term Source REF								
Person Last Name	Gutierrez							
Person First Name	Norma Carmen							
Person Mid Initials								
Person Email	normagu@usal.es							
Person Phone								
Person Fax								
Person Address	Hematology, University Hospital, Salamanca, 37007, Spain							
Person Affiliation	University Hospital							
Person Roles	submitter							
Person Roles Term Source REF	The MGED Ontology							
Quality Control Type								
Quality Control Term Source REF								
Replicate Type								
Replicate Term Source REF								
Normalization Type								
Normalization Term Source REF								
Date of Experiment								
Public Release Date	2007-08-14							
PubMed ID	15674361							
Publication DOI	15674361							
Publication Author List	N C Gutierrez, R Lopez-Perez, J M Hernandez, I Isidro, B Gonzalez, M Delgado, E Ferminan, J L Garcia, L Vazquez, M Gonzalez, J F San Miguel							
Publication Title	Gene expression profile reveals deregulation of genes with relevant functions in the different subclasses of acute myeloid leukemia.							
Publication Status	published							
Publication Status Term Source REF	EFO							
Publication Status Term Accession Number	EFO_0001796							
Experiment Description	Gene expression profile of acute myeloid leukemia. Bone marrow (BM) samples from 43 adult patients with newly de novo diagnosed AML.All samples contained more than 80% blast cells. Total RNA was extracted using Trizol reagent (Life Technologies, Gaithersburg, MD) and purified with RNeasy Mini Kit (Quiagen, Valencia, CA). The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). The labeled RNA was then fragmented and hybridazed to HU-133A oligonucleotide array (Affymetrix, Santa Clara, CA), which contained 22,283 probe sets, according to Affymetrix protocols. The arrays were scanned using Gene Array Scanner (Affymetrix).							
Protocol Name	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6						
Protocol Type	hybridization	feature_extraction						
Protocol Description	Title: Affymetrix Generic Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 						
Protocol Parameters								
Protocol Hardware								
Protocol Software	MicroArraySuite 5.0	MicroArraySuite 5.0						
Protocol Contact								
Protocol Term Source REF	mo							
SDRF File	E-GEOD-1729.sdrf.txt							
Term Source Name		nci_meta	NCI_thesaurus	The MGED Ontology	ArrayExpress	mo	EFO	The MGED Ontology
Term Source File		http://ncimeta.nci.nih.gov/indexMetaphrase.html	ncithesaurus.obo.alt	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php
Term Source Version