Investigation Title	Transcription profiling of human HEK 293 cells after storage at ambient temperature and rehydration	
Comment[Submitted Name]	Two week Storage and Rehydration of HEK 293 Cells at Ambient Temperature	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressReleaseDate]	2008-06-11	
Comment[SecondaryAccession]	GSE1455	
Comment[ArrayExpressAccession]	E-GEOD-1455	
Comment[MAGETAB TimeStamp_Version]	2010-08-01 16:07:10 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Helm	
Person First Name	Richard	
Person Mid Initials	F.	
Person Email	helmrf@vt.edu	
Person Phone	
Person Fax	
Person Address	Virginia Tech Center for Genomics, Biochemistry, Fralin Biotechnology Center, Virginia Polytechnic Institute and State University, West Campus Drive, Blacksburg, 24061, USA	
Person Affiliation	Virginia Polytechnic Institute and State University	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2008-06-11	
PubMed ID	16155929	
Publication DOI	16155929	
Publication Author List	Graham D Jack, E Andrew Mead, James F Garst, M Carla Cabrera, Andrea M DeSantis, Stephen M Slaughter, Jody Jervis, Andrew I Brooks, Malcolm Potts, Richard F Helm	
Publication Title	Long term metabolic arrest and recovery of HEK293 spheroids involves NF-kappaB signaling and sustained JNK activation.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	This series represents the rehydration series of the human 293h media depleted storage on agarose / rehydration condition course analysis. Samples include Control Monolayer,  0 hr desiccation, 0 hr rehydration, 6 hr rehydration, 24 hr rehydration, and 72 hr rehydration.	
Protocol Name	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6	
Protocol Type	hybridization	feature_extraction	
Protocol Description	Title: Affymetrix Generic Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software	MicroArraySuite 5.0	MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF	mo	
SDRF File	E-GEOD-1455.sdrf.txt	
Term Source Name	mo	The MGED Ontology	ArrayExpress	EFO	The MGED Ontology	mo	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version