Investigation Title	Transcription profiling of mouse spleen from AKR/J x FVB/NJ F1 versus DBA/2J x FVB F1	
Comment[Submitted Name]	(AKR/J x FVB/NJ)F1 versus (DBA/2J x FVB)F1 spleen expression data	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[SecondaryAccession]	GSE13225	
Comment[ArrayExpressReleaseDate]	2009-01-13	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressAccession]	E-GEOD-13225	
Comment[MAGETAB TimeStamp_Version]	2010-07-30 08:45:24 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Hunter	
Person First Name	Kent	
Person Mid Initials	
Person Email	hunterk@mail.nih.gov	
Person Phone	
Person Fax	
Person Address	Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37/5046 37 Convent Drive, Bethesda, 20892-4264, USA	
Person Affiliation	National Cancer Institute	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2009-01-13	
PubMed ID	
Publication DOI	
Publication Author List	
Publication Title	
Publication Status	
Publication Status Term Source REF	
Experiment Description	F1 hybrids from (AKR/J x FVB/NJ) and (DBA/2J x FVB/NJ) outcrosses display a 20-fold difference in mammary tumor metastatic capacity, due to differences in inherited polymorphisms.  Expression studies were performed to determine whether polymorphism-driven gene expression signatures predictive of outcome could be generated from normal tissues Experiment Overall Design: Spleen from adult F1 animals from (AKR/J x FVB/NJ) and (DBA/2J x FVB/NJ) outcrosses was collected and arrayed on Affymetrics chip to identify basal differences in gene expression between the different genotypes	
Protocol Name	P-G13225-1	P-G13225-3	P-G13225-2	P-AFFY-6	
Protocol Type	specified_biomaterial_action	nucleic_acid_extraction	labeling	feature_extraction	
Protocol Description	animals were euthanized by cervical dislocation under Avertin anesthesia.  Tissue was snap frozen in LN2 prior to RNA extraction	RNA was extracted by Trizol following the manufacturers suggested protocol.	Labeling was performed following the standard Affymetrix protocol	Title: Affymetrix CEL analysis. Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software				MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF	
SDRF File	E-GEOD-13225.sdrf.txt	
Term Source Name	NCBI Taxonomy	The MGED Ontology	ArrayExpress	EFO	The MGED Ontology	
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version