Investigation Title	Transcription profiling of mouse lymphoid and non-lymphoid leukemias induced by the Graffi murine leukemia retrovirus to obtain the cancerous signatures of T-cell, B-cell, erythroid and megakaryoblastic leukemias	
Comment[Submitted Name]	Gene expression profiles of the lymphoid and non-lymphoid leukemias induced by the Graffi murine leukemia retrovirus	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[SecondaryAccession]	GSE12581	
Comment[ArrayExpressReleaseDate]	2009-01-13	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressAccession]	E-GEOD-12581	
Comment[MAGETAB TimeStamp_Version]	2010-07-30 05:12:58 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Rassart	
Person First Name	Eric	
Person Mid Initials	
Person Email	rassart.eric@uqam.ca	
Person Phone	
Person Fax	
Person Address	Biology, UQAM, C.P. 8888 Succursale Centre-Ville, Montreal, H3C 3P8, Canada	
Person Affiliation	UQAM	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2009-01-13	
PubMed ID	
Publication DOI	
Publication Author List	
Publication Title	
Publication Status	
Publication Status Term Source REF	
Experiment Description	We used the microarrays to obtain the cancerous signatures of T-cell, B-cell, erythroid and megakaryoblastic leukemias in mice. Experiment Overall Design: 16 samples are included, 13 corresponding to leukemic samples and 3 corresponding to control samples. Each control sample corresponds to the pooled RNA of 12 mice.	
Protocol Name	P-G12581-1	P-G12581-3	P-G12581-2	P-AFFY-6	
Protocol Type	specified_biomaterial_action	nucleic_acid_extraction	labeling	feature_extraction	
Protocol Description	Inoculation of the Graffi GV-1.4 retrovirus in NFS mice	Trizol extraction of total RNA was performed according to the manufacturer's instructions. The quality was assessed using the Agilent Bioanalyzer.	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA	Title: Affymetrix CEL analysis. Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software				MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF	
SDRF File	E-GEOD-12581.sdrf.txt	
Term Source Name	NCBI Taxonomy	The MGED Ontology	ArrayExpress	EFO	The MGED Ontology	
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version