Comment[ArrayExpressAccession]	E-GEOD-12039
Investigation Title	Regulation of endothelial gene expression by miR-126 in human and zebrafish
Comment[Submitted Name]	Regulation of endothelial gene expression by miR-126 in human and zebrafish
Publication Title	miR-126 regulates angiogenic signaling and vascular integrity.
Publication Author List	Fish JE, Santoro MM, Morton SU, Yu S, Yeh RF, Wythe JD, Ivey KN, Bruneau BG, Stainier DY, Srivastava D
Publication DOI	
PubMed ID	18694566
Experimental Design	transcription profiling by array
Experimental Design Term Source REF	EFO
Public Release Date	2008-08-12
Experiment Description	This SuperSeries is composed of the following subset Series: GSE12011: Regulation of human endothelial gene expression by miR-126 GSE12012: Regulation of zebrafish endothelial gene expression by miR-126 Refer to individual Series																		
Date of Experiment																			
Term Source Name	EFO
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Person Last Name	Fish																		
Person First Name	Jason																		
Person Mid Initials	E.																		
Person Email	jefish@gladstone.ucsf.edu																		
Person Affiliation	J. David Gladstone Institute																		
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Person Address	Gladstone Institute of Cardiovascular Disease, J. David Gladstone Institute, 1650 Owens Street, San Francisco, California, U.S.A.																		
Person Roles	submitter																		
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Protocol Name	P-GSE12039-1	P-GSE12039-6	P-GSE12039-13	P-GSE12039-3	P-GSE12039-15	P-GSE12039-8	P-GSE12039-7	P-GSE12039-14	P-GSE12039-16	P-GSE12039-19	P-GSE12039-10	P-GSE12039-2	P-GSE12039-17	P-GSE12039-18	P-GSE12039-9	P-GSE12039-11	P-GSE12039-4	P-GSE12039-5	P-GSE12039-12
Protocol Description	ID_REF = <br>VALUE = normalized RMA signal (log2 scale)	Performed according to the manufacturer's (Affymetrix) recommendations	According to manufacturer's (Affymetrix) recommendations	Unstimulated	Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.	Raw intensities from the CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models. 	Performed according to the manufacturer's (Affymetrix) recommendations	According to manufacturer's (Affymetrix) recommendations	zebrafish embryos were injected at the one-cell stae with miR-126 antisense morpholino (4 ng, Gene-Tools), RNA isolated from 48 hpf fish	zebrafish embryos at the one cell stage were injected with miR-126 antisense morpholino (4 ng, Gene Tools), RNA isolated from 48 hpf fish	Uninjected control, 48 hpf	Electroporated with control morpholino (Gene-Tools, 15nmol), 72h	zebrafish embryos were injected at the one cell stage with miR-126 antisense morpholino (4 ng, Gene-Tools), RNA isolated from 48 hpf fish	RNA isolated from 48 hpf fish	Electroporated with miR-126 antisense morpholino (Gene-Tools, 15nmol), 72h	Trizol RNA extraction	Trizol extraction	Ambion MessageAmp II-biotin enhanced kit (manufacturer's recommendations)	Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
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Protocol Type	bioassay_data_transformation	hybridization	hybridization	grow	feature_extraction	feature_extraction	image_aquisition	image_aquisition	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	nucleic_acid_extraction	nucleic_acid_extraction	labeling	labeling
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Experimental Factor Name	ORGANISM																		
Experimental Factor Type	Organism																		
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Comment[SecondaryAccession]	GSE12039																		
Comment[GEOLastUpdateDate]	2008-12-05
Comment[GEOReleaseDate]	2008-08-11
Comment[ArrayExpressSubmissionDate]	2008-07-07
SDRF File	E-GEOD-12039.sdrf.txt