Comment[ArrayExpressAccession]	E-GEOD-11624
Public Release Date	2008-06-01													
Investigation Title	Drosophila melanogaster small RNA sequence from 14 libraries													
Comment[Submitted Name]	Drosophila melanogaster small RNA sequence from 14 libraries
Experiment Description	High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Small RNAs were sequenced from D. melanogaster heads (male and female), body (male and female), S2 and Kc cells and different stages of embryo. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt													
Date of Experiment														
Term Source Name	EFO
Term Source Version														
Term Source File	http://www.ebi.ac.uk/efo/efo.owl													
Person Last Name	Chung	Lai												
Person First Name	Wei-Jen	Eric												
Person Mid Initials		C												
Person Email	geo@ncbi.nlm.nih.gov													
Person Affiliation	Sloan-Kettering Institute													
Person Phone	212-6395549													
Person Fax														
Person Address	Developmental Biology, Sloan-Kettering Institute, 430 E 67th St, New York, NY, USA													
Person Roles	submitter													
Person Roles Term Source REF														
Person Roles Term Accession Number														
Normalization Type														
Normalization Term Accession Number														
Normalization Term Source REF														
Replicate Type														
Replicate Term Accession Number														
Replicate Term Source REF														
Experimental Design														
Experimental Design Term Accession Number														
Experimental Design Term Source REF														
Quality Control Type														
Quality Control Term Accession Number														
Quality Control Term Source REF														
Protocol Name	P-GSE11624-1	P-GSE11624-2	P-GSE11624-3	P-GSE11624-12	P-GSE11624-11	P-GSE11624-6	P-GSE11624-10	P-GSE11624-7	P-GSE11624-13	P-GSE11624-14	P-GSE11624-8	P-GSE11624-5	P-GSE11624-9	P-GSE11624-4
Protocol Description	SEQUENCE = <br>COUNT = number of times small RNA was sequenced	SEQUENCE = small RNA sequence<br>COUNT = number of times small RNA was sequenced	total RNA from female head, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	total RNA from 0-1hr embryo, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	total RNA from female body, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	total RNA from S2 cell, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	total RNA from male body, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	total RNA from KC cell, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	total RNA from 2-6hr embryo, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	total RNA from 6-10hr embryo, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	total RNA from imaginal disc, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	total RNA from S2 and KC cell, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	total RNA from male head, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt
Protocol Software														
Protocol Hardware														
Protocol Contact														
Protocol Type	bioassay_data_transformation	bioassay_data_transformation	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	nucleic_acid_extraction	feature_extraction
Protocol Term Source REF														
Protocol Term Accession Number														
Experimental Factor Name														
Experimental Factor Type														
Experimental Factor Term Source REF														
Experimental Factor Term Accession Number														
Publication Title	Endogenous RNA interference provides a somatic defense against Drosophila transposons.													
Publication Author List	Chung WJ, Okamura K, Martin R, Lai EC													
PubMed ID	18501606													
Publication DOI	10.1016/j.cub.2008.05.006													
Publication Status														
Publication Status Term Source REF														
Publication Status Term Accession Number														
Comment[SecondaryAccession]	GSE11624	SRP003879	SRP003876	SRP003801										
Comment[GEOLastUpdateDate]	2012-04-02
Comment[AEExperimentType]	RNA-seq of non coding RNA													
Comment[GEOReleaseDate]	2008-05-31
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/SRR069835-SRR069840													
Comment[ArrayExpressSubmissionDate]	2008-05-29
SDRF File	E-GEOD-11624.sdrf.txt