Comment[ArrayExpressAccession]	E-ERAD-238
MAGE-TAB Version	1.1
Investigation Title	Reprogramming around an epigenetic barrier with TET1
Comment[Submitted Name]	Reprogramming around an epigenetic barrier with TET1
Experiment Description	Mouse Epiblast Stem Cells (EpiSCs) were reprogrammed to induced pluripotent stem cells (iPSCs) by overexpression of key pluripotency factors and the TET1 DNA hydroxylase. 0.2ug of extractd DNA was sonicated using the Covaris Sonicator (300bp) and the Illumina library was prepared following a standard Illumina protocol with the NEB Next kit and subjected to bisulfite treament. The library was amplified 12x and fragments smaller than 200 bp were removed by PEG precipitation on magnetic beads. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Experimental Factor Name	sample description	timepoint	organism
Experimental Factor Type	sample description	timepoint	organism
Person Last Name	The Wellcome Trust Sanger Institute
Person Email	datahose@sanger.ac.uk
Person Address	Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
Person Affiliation	Wellcome Trust Sanger Institute
Person Roles	submitter
Public Release Date	2014-04-28
Protocol Name	SEQUENCING_PROTOCOL_1	LIBRARY_CONSTRUCTION_PROTOCOL_1
Protocol Type	nucleic acid sequencing protocol	nucleic acid library construction protocol
Protocol Term Source REF	EFO	EFO
Protocol Term Accession Number	EFO_0004170	EFO_0004184
Protocol Description	Illumina HiSeq 2000 software. Illumina HiSeq2000 flow cell preparation: PE-401-3001: TruSeq PE Cluster Kit v3 - cBot - HS; GD-401-3001: TruSeq SR Cluster Kit v3 - cBot - HS; FC-401-3001: TruSeq SBS Kit v3 - HS (200-cycles). http://www.illumina.com/technology/sequencing_technology.ilmn	qPCR only
Protocol Hardware	Illumina HiSeq 2000
Term Source Name	NCBI Taxonomy	EFO
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/	http://www.ebi.ac.uk/efo
Term Source Version		2.38
Comment[SecondaryAccession]	ERP004476
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/ERP004476
Comment[AEExperimentType]	methylation profiling by high throughput sequencing
Comment[SRASubmissionDate]	2013-12-17
Comment[AEExperimentDisplayName]	Reprogramming around an epigenetic barrier with TET1
SDRF File	E-ERAD-238.sdrf.txt