Array Design Name UMIST_S COELICOLOR_SC8_7337 Provider University of Manchester Institue of Science and Technology Comment[ArrayExpressAccession] A-MAXD-6 Comment[Description] Origin: Non-commercial
GAPS II slide Comment[SubmittedName] UMIST_S COELICOLOR_SC8_7337 Comment[Organism] Streptomyces coelicolor A3(2) Technology Type spotted_ds_DNA_features Technology Type Term Accession Number #spotted_ds_DNA_features Technology Type Term Source REF mo Surface Type aminosilane Surface Type Term Accession Number #aminosilane Surface Type Term Source REF mo Term Source Name mo entrez Term Source File http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ncbi.nih.gov/Entrez/ Term Source Version Comment[AdditionalFile:txt] A-MAXD-6_comments.txt [main] Block Column Block Row Column Row Reporter Name Control Type Composite Element Name Composite Element Database Entry[entrez] Composite Element Comment 4 1 2 8 [Reporter]SCO3891 (14P12)_PCR/1541 [Gene]SCO3891/1412 NC_003888 SCH24.13c, possible membrane protein, len: 282 aa; unknown function, previously sequenced as TR:O86857 (EMBL:AJ007313), ORF2. Contains hydrophobic, possible membrane-spanning region 4 1 2 7 [Reporter]SCO3329 (15L12)_PCR/1540 [Gene]SCO3329/1411 NC_003888 SCE68.27c, hypothetical protein, len: 271 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis, amino acid composition and possible RBS. The start codon is uncertain, alternatives being present at codons 19, 20 and 26 4 1 2 6 [Reporter]SCO1155 (16H12)_PCR/1539 [Gene]SCO1155/1410 NC_003888 SCG8A.09c, conserved hypothetical protein, len: 137aa; strongly similar to many from Streptomyces coelicolor eg. TR:Q9X835 (EMBL:AL049727) hypothetical 14.4 kD protein (139 aa) fasta scores; opt: 366, z-score: 458.5, E(): 4.3e-18, 48.0% identity in 127 aa overlap. Also strongly similar to neighbouring CDS SCG8A.13c. Contains Pfam match to entry PF01860 DUF42, Protein of unknown function 4 1 2 5 [Reporter]SCO1784 (17D12)_PCR/1538 [Gene]SCO1784/1409 NC_003888 SCI51.24, hypothetical protein, len: 115 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 4 1 2 4 [Reporter]SCO0603 (18P8)_PCR/1537 [Gene]SCO0603/1408 NC_003888 SCF55.27, probable amino transferase, len: 233 aa; similar to TR:P95530 (EMBL:D85415) Pseudomonas putida amino group transfer TdnT, 253 aa; fasta scores: opt: 251 z-score: 300.9 E(): 2.2e-09; 31.9% identity in 160 aa overlap. Contains match to Pfam entry PF00117 GATase, Glutamine amidotransferase class-I and to Prosite entry PS00442 Glutamine amidotransferases class-I active site 4 1 2 3 [Reporter]SCP1.251 (19L8)_PCR/1536 [Gene]SCP1.251/1407 NC_003888 SCP1.251, unknown, len: 122aa; 4 1 2 2 [Reporter]SCO4039 (20D8)_PCR/1535 [Gene]SCO4039/1406 NC_003888 2SCD60.05c, unknown, len: 182 aa 4 1 1 22 [Reporter]SCO5204 (1D4)_PCR/1533 [Gene]SCO5204/1405 NC_003888 SC7E4.01, hypothetical protein (fragment), len: >220 aa; similar to C-terminal part of TR:O32904 (EMBL:Z98271) Mycobacterium leprae hypothetical 29.1 kD protein MLCB1779.46, 277 aa; fasta scores: opt: 236 z-score: 242.2 E(): 5.1e-06; 33.5% identity in 191 aa overlap. Contains possible colied-coil region at aprox. residues 151..172 and a 5x degenerate repeat: (G/D/S)DK(2),2SC3B6.28, possible integral membrane protein (fragment), len: >815 aa; similar to TR:O53609 (EMBL:AL021428) Mycobacterium tuberculosis putative membrane protein MTV030.07, 979 aa; fasta scores: opt: 992 z-score: 1094.5 E(): 0; 41.1% identity in 829 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 1 21 [Reporter]SCO4916 (1P24)_PCR/1532 [Gene]SCO4916/1404 NC_003888 SCK13.08c, possible phosphomannomutase, len: 549 aa; similar to TR:O53360 (EMBL:AL021841) Mycobacterium tuberculosis putative phosphomannomutase MTV016.07, 534 aa; fasta scores: opt: 1378 z-score: 1522.6 E(): 0; 46.7% identity in 529 aa overlap. Contains Pfam match to entry PF00408 PGM_PMM, Phosphoglucomutase/phosphomannomutase and match to Prosite entry PS00710 Phosphoglucomutase and phosphomannomutase phosphoserine signature 4 1 1 20 [Reporter]SCO4569 (2L24)_PCR/1531 [Gene]SCO4569/1403 NC_003888 SCD16A.14c, nuoH, NADH dehydrogenase subunit, len: 467 aa; similar to many e.g. TR:P95173 (EMBL:Z83867) NuoH, NADH dehydrogenase subunit from Mycobacterium tuberculosis (410 aa) fasta scores; opt: 1630, z-score: 1664.7, E(): 0, (58.4% identity in 413 aa overlap). Contains Pfam match to entry PF00146 NADHdh, NADH dehydrogenases and a Prosite match to PS00667 Respiratory-chain NADH dehydrogenase subunit 1 signature 1. Contains possible hydrophobic membrane spanning regions 4 1 1 19 [Reporter]SCO6793 (3H24)_PCR/1530 [Gene]SCO6793/1402 NC_003888 SC1A2.02c, probable aldehyde dehydrogenase, len: 501 aa. Similar to many dehydrogenases e.g. Rhizobium sp. (strain NGR234) SW:GABD_RHISN(EMBL:AE000096) probable succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.16) (491 aa), fasta scores opt: 812 z-score: 864.8 E():0 33.3% identity in 468 aa overlap and Streptomyces coelicolor TR:Q9ZBH2(EMBL:AL035206) putative aldehyde dehydrogenase, SC9B5.08 (468 aa), fasta scores opt: 834 z-score: 888.4 E(): 0 37.1% identity in 463 aa overlap. Contains a Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family. Also contains Prosite hits to PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site. 4 1 1 18 [Reporter]SCO6202 (4D24)_PCR/1529 [Gene]SCO6202/1401 NC_003888 SC2G5.23c, unknown, len: 214aa 4 1 1 17 [Reporter]SCO7047 (5P20)_PCR/1528 [Gene]SCO7047/1400 NC_003888 SC4G1.13, probable transport integral membrane protein, len: 278 aa; similar to SW:BACA_ECOLI (EMBL:) Escherichia coli bacitracin resistance protein (putative undecaprenol kinase) (EC 2.7.1.66) BacA, 273 aa; fasta scores: opt: 483 z-score: 570.7 E(): 2.5e-24; 36.2% identity in 268 aa overlap. Contains possible hydrophobic membrane spanning regions 2 4 17 12 [Reporter]SCO5287 (11J5)_PCR/6914 [Gene]SCO5287/6299 NC_003888 SCCB12.11, possible MarR-family transcriptional regulator, len: 164 aa; similar to TR:Q9RL39 (EMBL: AL121596) Streptomyces coelicolor MarR-family protein SCF51.25, 148 aa; fasta scores: opt: 237 z-score: 290.2 E(): 1.1e-08; 37.6% identity in 141 aa overlap and to SW:YXAD_BACSU (EMBL:AB005554) Bacillus subtilis hypothetical transcriptional regulator on AsnH-GntR intergenic region YxaD, 143 aa; fasta scores: opt: 234 z-score: 286.9 E(): 1.6e-08; 35.7% identity in 140 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family and possible helix-turn-helix motif at residues 72..93 (+3.73 SD) 2 4 17 11 [Reporter]SCO4797 (12F5)_PCR/6913 [Gene]SCO4797/6298 NC_003888 SCD63A.08, probable ATP-dependent DNA helicase II, len: 831 aa; similar to TR:P73465 (EMBL:D90906) Synechocystis sp. DNA helicase II UvrD, 793 aa; fasta scores: opt: 1417 z-score: 1459.4 E(): 0; 40.4% identity in 817 aa overlap and to SW:REP_ECOLI (EMBL:M87049) Escherichia coli ATP-dependent DNA helicase Rep, 673 aa; fasta scores: opt: 824 z-score: 849.5 E(): 0; 37.8% identity in 661 aa overlap. Contains Pfam match to entry PF00580 UvrD-helicase, UvrD/REP helicase and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 4 17 10 [Reporter]SCO3061 (13B5)_PCR/6912 [Gene]SCO3061/6297 NC_003888 SCE25.02, possible integral membrane protein, len: 190 aa; similar to TR:P96882 (EMBL:Z92771) Mycobacterium tuberculosis hypothetical 30.1 kD protein MTCY71.17, 272 aa; fasta scores: opt: 197 z-score: 245.6 E(): 3.2e-06; 32.0% identity in 150 aa overlap. Contains possible hydrophobis membrane spanning regions 2 4 17 8 [Reporter]SCO3005 (15J1)_PCR/6910 [Gene]SCO3005/6296 NC_003888 SCE33.07c, secA, preprotein translocase, len: 947 aa; identical to SW:SECA_STRLI (EMBL:U21192) Streptomyces lividans preprotein translocase SecA subunit, 947 aa. Contains Pfam match to entry PF01043 SecA_protein, SecA protein, amino terminal region and match to Prosite entry PS01312 Protein secA signatures 2 4 17 7 [Reporter]SCO3764 (16F1)_PCR/6909 [Gene]SCO3764/6295 NC_003888 SCH63.11c, possible integral membrane protein, len: 341 aa; similar to TR:CAC01476 (EMBL:AL391014) Streptomyces coelicolor putative integral membrane protein 2SCG1.27, 337 aa; fasta scores: opt: 1316 z-score: 1472.5 E(): 0; 59.9% identity in 337 aa overlap. Contains Pfam match to entry PF01595 DUF21, Domain of unknown function DUF21 and 2x match to PF00571 CBS, CBS domain. Also contains possible hydrophobic membrane spanning regions 2 4 17 6 [Reporter]SCO3768 (17B1)_PCR/6908 [Gene]SCO3768/6294 NC_003888 SCH63.15, possible translocase protein, len: 82 aa; similar to SW:YK94_MYCTU (EMBL:Z73966) Mycobacterium tuberculosis hypothetical 8.9 kDa protein MTCY49.34c, 83 aa; fasta scores: opt: 123 z-score: 165.1 E(): 0.11; 40.6% identity in 69 aa overlap. Contains Pfam match to entry PF02416 MttA_Hcf106 2 4 17 5 [Reporter]SCO3934 (17N21)_PCR/6907 [Gene]SCO3934/6293 NC_003888 SCD78.01, ftsK /spoIIIE family protein, partial CDS within putative integrated plasmid, len: 271 aa; similar to many members of the ftsK /spoIIIE family e.g.SPI_STRAZ (EMBL:S58719) sporulation-regulatory protein from Streptomyces azureus plamsid pSA1.1 (303 aa), fasta scores; opt: 736 z-score: 1014.5 E(): 0, 49.0% identity in 247 aa overlap and TRSA_STRAM (EMBL:Z19593) transfer protein traSA from Streptomyces ambofaciens plasmid pSAM2 (306 aa), fasta scores; opt: 222 z-score: 676.5 E(): 2e-30, 47.8% identity in 203 aa overlap. Also similar to SC6A9.34, a protein within another putative integrated plasmid (on Streptomyces coelicolor cosmid St6A9)(E(): 5.4e-24, 45.7% identity in 247 aa overlap). Contains PS00017 ATP /GTP-binding site motif A (P-loop),SCQ11.17, ftsK/spoIIIE family protein, partial CDS, len: >200 aa; similar to many e.g. part of SW:TRSA_STRAM (EMBL:Z19593), traSA, Streptomyces ambofaciens plasmid pSAM2 transfer protein (415 aa), fasta scores; opt: 175 z-score: 218.8 E(): 7.4e-05, 34.5% identity in 116 aa overlap. Also similar to parts of CDS from other putative integrated plasmids in S.coelicolor e.g. SCE39.09c (EMBL:AL049573) korSA, regulatory protein (451 aa) (30.8% identity in 182 aa overlap) and TR:O86766 (EMBL:AL031035) probable traSA:integrase fusion protein (768 aa) (33.2% identity in 187 aa overlap). Contains TTA (leucine) codon, possible target for bldA regulation and PS00017 ATP/GTP-binding site motif A (P-loop. This CDS continues into cosmid D78 (EMBL:AL034355) as SCD78.01 2 4 17 4 [Reporter]SCP1.131 (18J21)_PCR/6906 [Gene]SCP1.131/6292 NC_003888 SCP1.131, unknown, len: 266aa; 2 4 17 3 [Reporter]SCP1.192 (19F21)_PCR/6905 [Gene]SCP1.192/6291 NC_003888 SCP1.192, unknown, len: 146aa; 2 4 17 2 [Reporter]SCO7503 (20N17)_PCR/6904 [Gene]SCO7503/6290 NC_003888 SCBAC17A6.36, putative extracellular solute-binding protein, len: 445aa; similar to many eg. TR:Q9L0B2 (EMBL:AL163003) putative sugar transporter sugar binding protein from Streptomyces coelicolor (452 aa) fasta scores: opt: 853, Z-score: 952.9, 37.275% identity (38.259% ungapped) in 389 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and a possible N-terminal signal sequence. 2 4 16 22 [Reporter]SCO1203 (1N13)_PCR/6902 [Gene]SCO1203/6289 NC_003888 2SCG58.03, possible MutT-like protein, len: 153 aa; similar to TR:CAB88476 (EMBL:AL353816) Streptomyces coelicolor putative MutT-like protein SCD84.10c, 142 aa; fasta scores: opt: 135 z-score: 171.1 E(): 0.046; 33.3% identity in 129 aa overlap. Contains match to Prosite entry PS00893 mutT domain signature 2 4 16 21 [Reporter]SCO5513 (2J13)_PCR/6901 [Gene]SCO5513/6288 NC_003888 SC8D9.25, ilvN, acetolactate synthase small subunit, len: 174 aa; high level of similarity to many e.g. TR:Q59817 (EMBL:L39268) IlvN, acetolactate synthase small subunit from the ilvBNC gene cluster of Streptomyces avermitilis (176 aa) fasta scores; opt: 884, z-score: 1056.9, E(): 0, (86.4% identity in 176 aa overlap). 2 4 16 20 [Reporter]SCO2214 (3F13)_PCR/6900 [Gene]SCO2214/6287 NC_003888 SC10B7.09, possible reductase, len: 366 aa; similar to TR:Q9ZA37 (EMBL:AJ011500) Streptomyces violaceoruber putative DTDP4-keto-6-deoxyhexose reductase, 304 aa; fasta scores: opt: 212 z-score: 236.4 E(): 8.7e-06; 31.6% identity in 285 aa overlap 2 4 16 19 [Reporter]SCO5473 (4B13)_PCR/6899 [Gene]SCO5473/6286 NC_003888 SC2A11.07, probable ATP/GTP binding protein, len: 222 aa; contains PS00017 ATP /GTP-binding site motif A (P-loop) 2 4 16 18 [Reporter]SCO2086 (5N9)_PCR/6898 [Gene]SCO2086/6285 NC_003888 SC4A10.19c, murD, UDP-N-acetylmuramoylalanine-D-glutamate ligase, len: 471 aa; previously partially sequenced as TR:Q9ZBA7 (EMBL:U10879), MurD, Streptomyces coelicolor UDP-N-acetylmuramoylalanine-D-glutamate ligase (fragment) (221 aa) and identical to that sequence. Similar to many e.g. TR:O68388 (EMBL:AF035938), MurD, Streptococcus pyogenes UDP-N-acetylmuramoylalanine-D-glutamate ligase (452 aa), fasta scores; opt: 399 z-score: 431.5 E(): 1.1e-16, 33.3% identity in 469 aa overlap. Shows weak similarity to SC9B1.07 (EMBL:AL049727) S.coelicolor possible UDP-N-acetylmuramyl-L-alanine ligase (462 aa) (27.9% identity in 480 aa overlap). Contains Pfam match to entry PF01225 Mur_ligase, Mur ligase family 2 4 16 17 [Reporter]SCO7455 (6J9)_PCR/6897 [Gene]SCO7455/6284 NC_003888 SC5C11.12, possible isochorismatase (EC 3.3.2.1), len: 178 aa. Similar to several including: Escherichia coli SW:ENTB_ECOLI(EMBL:M24148) isochorismatase (EC 3.3.2.1), EntB, involved in enterobactin biosynthesis (285 aa), fasta scores opt: 210 z-score: 266.3 E(): 2.1e-07 26.3% identity in 179 aa overlap. Contains a Pfam match to entry PF00857 Isochorismatase, Isochorismatase family. 2 4 16 16 [Reporter]SCO5681 (7F9)_PCR/6896 [Gene]SCO5681/6283 NC_003888 SC5H4.05c, possible glycosyl transferase, len: 391 aa; similar to TR:Q9X7Z1 (EMBL:AL049497) Streptomyces coelicolor putative glycosyl transferase SC6G10.05c, 412 aa; fasta scores: opt: 1364 z-score: 1557.1 E(): 0; 56.4% identity in 385 aa overlap. Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1 2 4 16 15 [Reporter]SCO5321 (8B9)_PCR/6895 [Gene]SCO5321/6282 NC_003888 SC6G9.12c, polyketide hydroxylase, len: 627 aa; previously partially sequenced from S.coelicolor as SW:HYDL_STRCO (EMBL:X74213). Highly similar to SW:HYDL_STRHA (EMBL:L05390), schC, Streptomyces halstedii putative polyketide hydroxylase involved in synthesis of a polyketide spore pigment (555 aa), fasta scores; opt: 2290 z-score: 1755.0 E(): 0, 69.2% identity in 629 aa overlap. Also similar to many hydroxylases of substitued aromatic compounds. A notable difference between this sequence and its Streptomyces halstedii homologue is the presence of 12x GGP repeats starting at aa 413. Weakly similar to TR:O50491 (EMBL:AL020958) S.coelicolor possible monooxygenase (541 aa) (29.2% identity in 583 aa overlap). This feature is also absent from the other related hydroxylases. Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase. Contains possible hydrophobic membrane spanning region 2 4 16 14 [Reporter]SCO7828 (9N5)_PCR/6894 [Gene]SCO7828/6281 NC_003888 SC8E7.25, possible DNA-binding protein, len: 84 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1X4 (EMBL:AL109973) hypothetical 9.0 kd protein, SCJ30.15C (84 aa). Contains a putative helix-turn-helix motif situated between residues 1..22 (+2.56 SD). 2 4 16 13 [Reporter]SCO2787 (10J5)_PCR/6893 [Gene]SCO2787/6280 NC_003888 SCC105.18, hypothetical protein, len: 298 aa; similar to TR:Q9RDQ8 (EMBL:AL133423) Streptomyces coelicolor hypothetical 31.7 kD protein SC4A7.04, 295 aa; fasta scores: opt: 556 z-score: 623.0 E(): 2.9e-27; 37.4% identity in 294 aa overlap 2 4 16 12 [Reporter]SCO2280 (11F5)_PCR/6892 [Gene]SCO2280/6279 NC_003888 SCC75A.26c, possible TetR-family transcriptional regulator, len: 215 aa. Similar to several other regulators including: Pseudomonas fluorescens TR:AAD31815(EMBL:AF129856) transcriptional regulatory protein PhlF (208 aa), fasta scores opt: 198 z-score: 248.3 E(): 1.9e-06 25.9% identity in 158 aa overlap and Streptomyces coelicolor TR:CAB56137(EMBL:AL117669) putative transcriptional regulator SCF12.11 (206 aa), fasta scores opt: 309 z-score: 381.8 E(): 6.8e-14 30.5% identity in 213 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 48..69. 2 4 16 11 [Reporter]SCO4793 (12B5)_PCR/6891 [Gene]SCO4793/6278 NC_003888 SCD63A.04c, probable NPL/P60 family secreted protein, len: 347 aa; similar to TR:Q9XAQ3 (EMBL:AL078618) Streptomyces coelicolor SCD16A.22, 277 aa; fasta scores: opt: 409 z-score: 418.8 E(): 7.3e-16; 34.8% identity in 250 aa overlap, to TR:Q9X7Z5 (EMBL:AL049497) Streptomyces coelicolor putative secreted protein SC6G10.09c, 347 aa; fasta scores: opt: 928 z-score: 800.9 E(): 0; 44.2% identity in 353 aa overlap and to Streptomyces coelicolor SCD63A.07c, 398 aa. Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family and possible N-terminal region signal peptide sequence. Contains also possible colied-coil region at aprox. residues 58..98 2 4 16 10 [Reporter]SCO3024 (13N1)_PCR/6890 [Gene]SCO3024/6277 NC_003888 SCE34.05c, possible transport protein, len: 326 aa; similar to TR:AAF26917 (EMBL:AF210843) Sorangium cellulosum putative transport protein, 305 aa; fasta scores: opt: 629 z-score: 707.7 E(): 5.9e-32; 37.8% identity in 299 aa overlap. Contains Pfam match to entry PF01545 Cation_efflux, Cation efflux family and possible hydrophobic membrane spanning regions 2 4 16 9 [Reporter]SCO3405 (14J1)_PCR/6889 [Gene]SCO3405/6276 NC_003888 SCE9.12c, hprT, probable hypoxanthine phosphoribosyltransferase, len: 187 aa; similar to many e.g. TR:O33799 (EMBL:AF008931), HprT, Salmonella typhimurium hypoxanthine phosphoribosyltransferase (178 aa), fasta scores; opt: 583 z-score: 711.2 E(): 2.7e-32, 54.2% identity in 166 aa overlap. The start codon is uncertain as there are 5 potential. Contains Pfam match to entry PF00156 Pribosyltran, Purine/pyrimidine phosphoribosyl transferases, score 177.70, E-value 1.9e-49 and PS00103 Purine/pyrimidine phosphoribosyl transferases signature. Contains probable coiled-coil from 3 to 39 (37 residues) (Max score: 1.481, probability 0.93) 2 4 16 8 [Reporter]SCO0833 (15F1)_PCR/6888 [Gene]SCO0833/6275 NC_003888 SCF43A.23c, unknown, len: 236 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 2 4 16 7 [Reporter]SCO3427 (16B1)_PCR/6887 [Gene]SCO3427/6274 NC_003888 SCE9.34c, rpmE, probable 50S ribosomal protein L31, len: 84 aa; similar to many e.g. SW:RK31_GUITH (EMBL:U81044) Guillardia theta chloroplast 50S ribosomal protein L31 (72 aa), fasta scores; opt: 149 z-score: 220.0 E(): 6.2e-05, 48.8% identity in 43 aa overlap. Contains Pfam match to entry PF01197 Ribosomal_L31, Ribosomal protein L31, score 59.20, E-value 8.7e-14 and PS01143 Ribosomal protein L31 signature 2 4 16 6 [Reporter]SCO0364 (16N21)_PCR/6886 [Gene]SCO0364/6273 NC_003888 SCF41.23, possible enoyl-CoA hydratase, len: 268 aa. Shares a high level of similarity with Mycobacterium tuberculosis TR:O53418 (EMBL; AL021897) enoyl-CoA hydratase MTV017.23C (EMBL; AL021897), fasta scores opt: 554 z-score: 635.0 E(): 5.1e-28 40.9% identity in 257 aa overlap. Contains a Pfam match to entry PF00378 ECH, Enoyl-CoA hydratase/isomerase family 2 4 16 5 [Reporter]SCO1623 (17J21)_PCR/6885 [Gene]SCO1623/6272 NC_003888 SCI41.06, unknown, len: 280 aa; similar to e.g. YX26_MYCTU hypothetical 30.6 KD protein CY274.26C, 283 aa; fasta scores: opt: 641 z-score: 744.6 E()0; 45.9% identity in 294 aa overlap 1 3 22 5 [Reporter]SCO1541 (18A6)_PCR/4498 [Gene]SCO1541/4099 NC_003888 SCL2.31, probable regulator, len: 159 aa; similar to various Streptomyces regulatory proteins, e.g. TR:CAB51005 (EMBL:AL096852) Streptomyces coelicolor putative regulator SCE19A.24, 142 aa; fasta scores: opt: 503 z-score: 661.7 E(): 1.8e-29; 57.9% identity in 133 aa overlap and TR:P95753 (EMBL:D50051) Streptomyces griseus SsgA, 136 aa; fasta scores: opt: 326 z-score: 433.9 E(): 8.9e-17; 43.1% identity in 130 aa overlap 2 4 16 4 [Reporter]SCO3753 (18F21)_PCR/6884 [Gene]SCO3753/6271 NC_003888 SCH22A.31, possible integral membrane, len: 850 aa. Contains possible hydrophobic membrane spanning regions 1 3 22 4 [Reporter]SCP1.76c (19M2)_PCR/4497 [Gene]SCP1.76c/4098 NC_003888 SCP1.76c, unknown, len: 181aa; 2 4 16 3 [Reporter]SCO1381 (19B21)_PCR/6883 [Gene]SCO1381/6270 NC_003888 SC1A8A.01c, partial CDS, hypothetical protein, len: >183aa; similar to SW:Q50603 (Y0DD_MYCTU) hypothetical protein from Mycobacterium tuberculosis (225 aa) fasta scores; opt: 641, z-score: 633.8, E(): 7.1e-28, 64.7% identity in 150 aa overlap. Note possible alternative translational start sites downstream.,SC10A9.23c, conserved hypothetical protein (fragment), len: >78 aa; similar to SW:YI30_MYCTU (EMBL:Z78020) Mycobacterium tuberculosis hypothetical 24.0 kDa protein MTCY1A11.13c, 225 aa; fasta scores: opt: 269 z-score: 377.2 E(): 1.4e-13; 56.0% identity in 75 aa overlap 1 3 22 3 [Reporter]SCO1316 (20I2)_PCR/4496 [Gene]SCO1316/4097 NC_003888 SCBAC36F5.27c, possible integral membrane protein, len: 211 aa; similar to SW:YQ37_MYCTU (EMBL:Z80225) Mycobacterium tuberculosis hypothetical 23.0 kDa protein RV2637, 218 aa; fasta scores: opt: 833 Z-score: 928.0 bits: 178.8 E(): 4.4e-44; 58.706% identity in 201 aa overlap. Contains Pfam match to entry PF00597 DedA, DedA family and match to Prosite entry PS00606 Beta-ketoacyl synthases active site. Also contains possible hydrophobic membrane spanning regions 1 3 21 22 [Reporter]SCO1143 (1A22)_PCR/4493 [Gene]SCO1143/4096 NC_003888 2SCG38.36c, conserved hypothetical protein, len: 253 aa; similar to TR:O66972 (EMBL:AE000707) Aquifex aeolicus hypothetical 38.3 kDa protein AQ_775, 326 aa; fasta scores: opt: 376 z-score: 435.0 E(): 8.8e-17; 32.8% identity in 235 aa overlap 1 3 21 21 [Reporter]SCO5006 (2M18)_PCR/4492 [Gene]SCO5006/4095 NC_003888 SCK15.08, minD1, possible septum site-determining protein, len: 418 aa; N-terminal domain similar to TR:Q9RZD9 (EMBL:AE001862) Deinococcus radiodurans DNA-binding response regulator DRA0010, 211 aa; blastp scores: Score = 103 (36.3 bits), Expect = 0.0045, Identities = 50/194 (25%), Positives = 85/194 (43%) and C-terminal domain highly similar to TR:Q9ZF15 (EMBL:AJ005576) Streptomyces griseus septum site-determining protein MinD protein, 396 aa; fasta scores: opt: 1230 z-score: 1302.8 E(): 0; 70.5% identity in 281 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00991 ParA, ParA family ATPase 1 3 21 20 [Reporter]SCO6419 (3I18)_PCR/4491 [Gene]SCO6419/4094 NC_003888 SC1A6.08c, unknown, len: 218 aa 1 3 21 19 [Reporter]SCO5607 (4E18)_PCR/4490 [Gene]SCO5607/4093 NC_003888 SC2E1.24, possible prophage transcriptional regulator, len:64 aa; similar to the N-terminus of several regulatory proteins e.g. Streptomyces phaeochromogenes plasmid pJV1 TR:Q54677 (EMBL:U23762) traR (245 aa), fasta scores; opt:137 z-score: 268.5 E(): 1e-07, 39.3% identity in 61 aa overlap. Contains Pfam match to entry gntR PF00392, Bacterial regulatory proteins, gntR family, score 43.92 1 3 21 18 [Reporter]SCO2411 (5A18)_PCR/4489 [Gene]SCO2411/4092 NC_003888 SC4A7.39c, possible membrane protein, len: 184 aa; similar to various hypothetical proteins, e.g. SW:Y07R_MYCTU (EMBL:Z75555) Mycobacterium tuberculosis hypothetical 28.3 kD protein, 261 aa; fasta scores: opt: 188 z-score: 223.5 E(): 4.6e-05; 28.7% identity in 164 aa overlap and to S/ coelicolor SC4A7.41c, >165 aa; fasta scores: opt: 240 z-score: 255.3 E(): 7.9e-09; 36.0% identity in 161 aa overlap. Contains possible hydrophobic membrane spanning region 1 3 21 17 [Reporter]SCO6750 (6M14)_PCR/4488 [Gene]SCO6750/4091 NC_003888 SC5F2A.33c, possible IPP isomerase, len: 197 aa; similar to many e.g. SW:BCH4_RHOCA bacteriochlorophyll synthase (176 aa) fasta scores; opt: 354, z-score: 431.9, E(): 9.6e-17, (42.0% identity in 157 aa overlap) and SW:IPPI_SCHPO isopentenyl diphosphate isomerase from Schizosaccharomyces pombe (227 aa) fasta scores; opt: 219, z-score: 270.0, E(): 9.9e-08, (36.0% identity in 178 aa overlap). 1 1 11 3 [Reporter]SCP1.175 (19M16)_PCR/222 [Gene]SCP1.175/199 NC_003888 SCP1.175, possible membrane protein, len: 147aa; contains a possible membrane-spanning hydrophobic region. 1 3 21 16 [Reporter]SCO1418 (7I14)_PCR/4487 [Gene]SCO1418/4090 NC_003888 SC6D7.21c, possible integral membrane protein, len: 230 aa. Similar to several proteins of undefined function e.g. Haemophilus influenzae SW:Y522_HAEIN (EMBL:U32734) hypothetical protein HI0522 (218 aa), fasta scores opt: 492 z-score: 566.3 E(): 3.6e-24 39.1% identity in 207 aa overlap and Bacillus subtilis SW:YYAS_BACSU(EMBL:D26185) hypothetical 22.0 KD protein (201 aa), fasta scores opt: 232 z-score: 273.7 E(): 7.2e-08 26.5% identity in 181 aa overlap. Contains a 3xSRQ 1x SGQ repeat region in the N-terminus 1 1 11 2 [Reporter]SCO5088 (20E16)_PCR/221 [Gene]SCO5088/198 NC_003888 SCBAC28G1.14, actIORF2, actinorhodin polyketide beta-ketoacyl synthase beta subunit (also known as chain length factor), len: 407 aa; identical to previously sequenced SW:KASB_STRCO (EMBL:X63449) Streptomyces coelicolor actinorhodin polyketide putative beta-ketoacyl synthase 2 ActI ORF2, 407 aa. Contains Pfam matches to entries PF00109 ketoacyl-synt, Beta-ketoacyl synthase, N-terminal domain and PF02801 ketoacyl-synt_C, Beta-ketoacyl synthase, C-terminal domain 1 1 10 22 [Reporter]SCO2807 (1E12)_PCR/219 [Gene]SCO2807/197 NC_003888 2SCC13.15, possible integral membrane protein, len: 329 aa; similar to TR:CAB90881 (EMBL:AL355752) Streptomyces coelicolor putative integral membrane protein SC10B7.28, 287 aa; fasta scores: opt: 598 z-score: 630.9 E(): 1.2e-27; 37.7% identity in 284 aa overlap. Contains possible hydrophobic membrane spanning regions 1 1 10 21 [Reporter]SCO1498 (2A12)_PCR/218 [Gene]SCO1498/196 NC_003888 SC9C5.22c, aroE, shikimate 5-dehydrogenase, len: 255 aa; similar to SW:AROE_PSEAE (EMBL:X85015) Pseudomonas aeruginosa shikimate 5-dehydrogenase (EC 1.1.1.25) AroE, 274 aa; fasta scores: opt: 294 z-score: 335.8 E(): 3.1e-11; 31.3% identity in 259 aa overlap. Contains Pfam match to entry PF01488 Shikimate_DH, Shikimate / quinate 5-dehydrogenase 1 1 10 20 [Reporter]SCO5217 (3M8)_PCR/217 [Gene]SCO5217/195 NC_003888 SC7E4.14, rsrA, anti-sigma factor, len: 105 aa; identical to previously sequenced TR:Q9RL96 (EMBL:AJ010320) Streptomyces coelicolor anti-sigma factor RsrA, 105 aa 1 1 10 19 [Reporter]SCO2253 (4I8)_PCR/216 [Gene]SCO2253/194 NC_003888 SC1G2.15c, unknown, len: 258 aa. Similar to several proteins of undefined function from Streptomyces coelicolor including: TR:O86676(EMBL:AL031371) SC4G2.03 (277 aa), fasta scores opt: 758 z-score: 919.8 E():0 46.7% identity in 276 aa overlap and TR:Q9X8M2(EMBL:AL049628) SCE94.16C (280 aa), fasta scores opt: 476 z-score: 580.1 E(): 6.2e-25 32.7% identity in 260 aa overlap. This gene is duplicated on this cosmid and shares 76.1 % identity, in 259 aa overlap, with the upstream gene, SC1G2.08c. 1 1 10 18 [Reporter]SCO6386 (5E8)_PCR/215 [Gene]SCO6386/193 NC_003888 SC3C8.05, unknown, questionable ORF, len: 127 aa; contains TTA leucine codon; possible target for action of bldA 1 1 10 17 [Reporter]SCO2056 (6A8)_PCR/214 [Gene]SCO2056/192 NC_003888 SC4G6.25, unknown, len: 376aa; 1 1 10 16 [Reporter]SCO3622 (7M4)_PCR/213 [Gene]SCO3622/191 NC_003888 SC66T3.33, probable aminotransferase, len: 451 aa; similar to many e.g. SW:GABT_ECOLI (EMBL:M88334), gabT, Escherichia coli 4-aminobutyrate aminotransferase (426 aa), fasta scores; opt: 706 z-score: 815.4 E(): 0, 32.3% identity in 427 aa overlap. Also similar to TR:O86744 (EMBL:AL031035) S.coelicolor probable aminotransferase (457 aa) (37.3% identity in 362 aa overlap). Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate and PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site 1 1 10 15 [Reporter]SCO5582 (8I4)_PCR/212 [Gene]SCO5582/190 NC_003888 SC7A1.26, possible regulator, len: 500aa; similar to TR:Q53958 (EMBL:M80614) sporulation-associated protein from Streptomyces coelicolor (473 aa) fasta scores; opt: 612, z-score: 589.9, E(): 1.5e-25, (31.3% identity in 485 aa overlap) and TR:E1319689 (EMBL:AL031541) putative transcriptional regulator from Streptomyces coelicolor (446 aa) fasta scores; opt: 279, z-score: 413.7, E(): 9.5e-16, (25.9% identity in 478 aa overlap) 2 4 16 2 [Reporter]SCO7471 (20J17)_PCR/6882 [Gene]SCO7471/6269 NC_003888 SCBAC17A6.04, possible phenylacetic acid degradation protein PaaA, len: 328 aa: similar to many e.g. SW:P76077 (PAAA_ECOLI) phenylacetic acid degradation protein PaaA from Escherichia coli (309 aa) fasta scores; opt: 1540, Z-score: 1801.2, 69.967% identity (69.967% ungapped) in 303 aa overlap. 2 4 15 22 [Reporter]SCO1105 (1J13)_PCR/6880 [Gene]SCO1105/6268 NC_003888 2SCG4.21c, possible secreted protein, len: 500 aa. Contains possible N-terminal region signal peptide sequence 2 4 15 21 [Reporter]SCO5587 (2F13)_PCR/6879 [Gene]SCO5587/6267 NC_003888 SC2E1.04, ftsH, cell division protein FtsH homolog (zinc metalloprotease, integral membrane protein), len: 648 aa; similar to many e.g. FTSH_LACLA (695 aa), fasta scores; opt: 1489 z-score: 1195.5 E(): 0, 41.7% identity in 640 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00674 AAA-protein family signature and Pfam matches to entry AAA PF00004, ATPases associated with various cellular activities (AAA) and to entry zn-protease PF00099, Zinc-binding metalloprotease domain, score 22.07, score 357.96. Proline-rich N-terminus is not present in other ftsH homologs 2 4 15 20 [Reporter]SCO2208 (3B13)_PCR/6878 [Gene]SCO2208/6266 NC_003888 SC10B7.03c, probable carboxylesterase, len: 290 aa; identical to previously sequenced TR:Q9X956 (EMBL:Y13833) Streptomyces coelicolor hypothetical 30.8 kD protein, 290 aa and similar to TR:Q59248 (EMBL:X82892) Bacillus subtilis carboxylesterase NP (EC 3.1.1.1) Nap, 300 aa; fasta scores: opt: 438 z-score: 516.3 E(): 2.2e-21; 31.5% identity in 292 aa overlap 2 4 15 18 [Reporter]SCO2082 (5J9)_PCR/6876 [Gene]SCO2082/6265 NC_003888 SC4A10.15c, ftsZ, cell division protein, len: 399 aa; previously sequenced as SW:FTSZ_STRCO (EMBL:U10879), ftsZ, Streptomyces coelicolor cell division protein (399 aa) and identical that sequence. Contains PS01134 FtsZ protein signature 1 and PS01135 FtsZ protein signature 2 2 4 15 17 [Reporter]SCO5781 (6F9)_PCR/6875 [Gene]SCO5781/6264 NC_003888 SC4H2.02, putative secreted protein, len: 179 aa; Proline-rich in N-terminus. Contains possible N-terminal region signal peptide sequence 2 4 15 16 [Reporter]SCO7251 (7B9)_PCR/6874 [Gene]SCO7251/6263 NC_003888 SC5H1.41, hypothetical protein, len: 345 aa; unknown function, similar to part of TR:Q9Z4Z7 (EMBL:AL035707) S.coelicolor putative phosphotransferase SCE29.03 (300 aa), fasta scores; opt: 433 z-score: 437.4 E(): 4.9e-17, 37.2% identity in 269 aa overlap. Contains TTA (leucine) codon, possible target for bldA regulation. There are imperfect inverted repeats at each end of the CDS 2 4 15 14 [Reporter]SCO7823 (9J5)_PCR/6872 [Gene]SCO7823/6262 NC_003888 SC8E7.20c, possible membrane protein, len: 136 aa. Contains possible membrane spanning hydrophobic domains. 1 3 21 15 [Reporter]SCO5220 (8E14)_PCR/4486 [Gene]SCO5220/4089 NC_003888 SC7E4.17, unknown, len: 326 aa 2 4 15 13 [Reporter]SCO5136 (10F5)_PCR/6871 [Gene]SCO5136/6261 NC_003888 SC9E12.21, probable aminotransferase, len: 365 aa; similar to TR:O50434 (EMBL:AL010186) Mycobacterium tuberculosis aminotransferase MTV005.14, 362 aa; fasta scores: opt: 1448 z-score: 1704.1 E(): 0; 61.7% identity in 360 aa overlap. Contains match to Prosite entry PS00105 Aminotransferases class-I pyridoxal-phosphate attachment site 1 3 21 14 [Reporter]SCO5419 (9A14)_PCR/4485 [Gene]SCO5419/4088 NC_003888 SC8F4.23, trxA4, possible thioredoxin, len: 318aa; similar to many e.g. SW:P00275 (THI1_CORNE) thioredoxin C-1 from Corynebacterium nephridii (105 aa) fasta scores; opt: 297, z-score: 317.6, E(): 2.8e-10, 44.3% identity in 97 aa overlap. Contains Pfam match to entry PF00085 thiored, Thioredoxin and Prosite match to PS00194 Thioredoxin family active site. 2 4 15 12 [Reporter]SCO2276 (11B5)_PCR/6870 [Gene]SCO2276/6260 NC_003888 SCC75A.22, possible membrane protein, len: 420 aa. Similar to several proteins of undefined function including: Bacillus subtilis SW:YWBN_BACSU(EMBL:X73124) (416 aa), fasta scores opt: 1076 z-score: 1183.4 E(): 0 42.7% identity in 405 aa overlap and Streptomyces coelicolor TR:Q9ZBW9(EMBL:AL034355) SCD78.30c (445 aa), fasta scores opt:584 z-score: 643.8 E(): 1.7e-28 43.2% identity in 428 aa overlap. Contains a possible N-terminal membrane spanning hydrophobic domain. 1 3 21 13 [Reporter]SCO2570 (10M10)_PCR/4484 [Gene]SCO2570/4087 NC_003888 SCC123.08c, possible secreted protein, len: 258 aa. Contains a possible N-terminal signal sequence. 1 3 21 12 [Reporter]SCO4328 (11I10)_PCR/4483 [Gene]SCO4328/4086 NC_003888 SCD12A.11c, possible lipoprotein, len: 154 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 1 3 21 11 [Reporter]SCO4168 (12E10)_PCR/4482 [Gene]SCO4168/4085 NC_003888 SCD66.05, probable oxidoreductase, len: 275 aa; similar to TR:BAA95121 (EMBL:AB042262) Corynebacterium aquaticum levodione reductase Lvr, 267 aa; fasta scores: opt: 477 z-score: 478.6 E(): 3.5e-19; 37.3% identity in 260 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 1 3 21 10 [Reporter]SCO3246 (13A10)_PCR/4481 [Gene]SCO3246/4084 NC_003888 SCE29.15c, fabH4, probable 3-oxoacyl-[acyl carrier protein] synthase III, len: aa; similar to many eg. SW:FABH_ECOLI 3-oxoacyl-[acyl carrier protein] synthase III, FabH, from Escherichia coli (317 aa) fasta scores; opt: 765, z-score: 862.6, E(): 0, (40.8% identity in 314 aa overlap). Also similar to two from Streptomyces coelicolor - TR:O54151 (EMBL:AL021409) (335 aa) fasta scores; opt: 694, z-score: 782.9, E(): 0, (53.5% identity in 331 aa overlap) and TR:E1359140 (EMBL:AL034443) (316 aa) fasta scores; opt: 685, z-score: 773.2, E(): 0, (40.6% identity in 313 aa overlap). 1 3 21 9 [Reporter]SCO2770 (14M6)_PCR/4480 [Gene]SCO2770/4083 NC_003888 SCC105.01c, speB, agmatinase (fragment), len: >302 aa; highly similar to N-terminal region of SW:SPEB_STRCL (EMBL:L15470) Streptomyces clavuligerus possible agmatinase (EC 3.5.3.11) Pah, 313 aa; fasta scores: opt: 843 z-score: 968.6 E(): 0; 43.3% identity in 293 aa overlap and of SW:SPEB_ECOLI (EMBL:M32363) Escherichia coli agmatinase (EC 3.5.3.11), SpeB, 306 aa; fasta scores: opt: 473 z-score: 546.7 E(): 5.1e-23; 41.8% identity in 282 aa overlap. Contains Pfam match to entry PF00491 arginase, Arginase family and matches to Prosite entries PS00147 Arginase family signature 1, PS00148 Arginase family signature 2, PS00678 Trp-Asp (WD) repeats signature and PS01053 Arginase family signature 3,SCC57A.41c, possible agmatinase, partial CDS, len: >53 aa. Highly similar to Escherichia coli SW:SPEB_ECOLI(EMBL:M32363) agmatinase (EC 3.5.3.11) (306 aa), fasta scores opt: 145 z-score: 242.9 E(): 3.8e-06 49.0% identity in 49 aa overlap and Schizosaccharomyces pombe SW:SPEB_SCHPO(EMBL:Z68166) putative agmatinase precursor (EC 3.5.3.11) (394 aa), fasta scores opt: 159 z-score: 263.7 E(): 2.7e-07 55.6% identity in 45 aa overlap. 1 3 21 8 [Reporter]SCO3902 (15I6)_PCR/4479 [Gene]SCO3902/4082 NC_003888 SH24.24, probable integral membrane protein, len: 502 aa; unknown function, similar to putative transmembrane proteins from Mycobacteria e.g. TR:O53127 (EMBL:AL022118) Mycobacterium leprae putative transmembrane protein (564 aa), fasta scores; opt: 520 z-score: 575.6 E(): 9.5e-25, 38.4% identity in 490 aa overlap. Contains several hydrophobic, probable membrane-spanning regions 1 3 21 7 [Reporter]SCO1691 (16E6)_PCR/4478 [Gene]SCO1691/4081 NC_003888 SCI30A.12c, possible tetR transcriptional regulator, len: 251 aa; similar to many e.g. SW:BM3R_BACME transcriptional repressor from Bacillus megaterium (192 aa) fasta scores; opt: 190, z-score: 227.2, E(): 2.5e-05, (26.7% identity in 176 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and Prosite match to PS01081 Bacterial regulatory proteins, tetR family signature. Contains possible helix-turn-helix motif (+3.97 SD) 87-108aa. 1 1 10 14 [Reporter]SCO7716 (9E4)_PCR/211 [Gene]SCO7716/189 NC_003888 SC8D11.07, hypothetical protein, len: 151 aa; similar to TR:Q9L188 (EMBL:AL158057) Streptomyces coelicolor hypothetical 18.3 kDa protein SC10G8.14, 168 aa; fasta scores: opt: 428 z-score: 542.8 E(): 1.1e-22; 46.8% identity in 139 aa overlap 1 3 21 6 [Reporter]SCO3359 (17A6)_PCR/4477 [Gene]SCO3359/4080 NC_003888 SCE94.10, cseC, sensory histidine kinase, len: 507 aa; previously sequenced therefore identical to TR:E1358528 (EMBL:AJ131213) CseC, sensory histidine kinase from Streptomyces coelicolor (507 aa) fasta scores; opt: 3298, z-score: 3217.4, E(): 0, (100.0% identity in 507 aa overlap). Also similar to many others eg. SW:AFQ2_STRCO sensor protein, part of a putative two-component regulatory system involved in secondary metabolism in Streptomyces coelicolor (535 aa) fasta scores; opt: 382, z-score: 378.4, E(): 9.2e-14, (29.8% identity in 413 aa overlap). Contains Pfam matches to entry PF00672 DUF5, Domain found in bacterial signal proteins and entry PF00512 signal, Signal carboxyl-terminal domain. 1 1 10 13 [Reporter]SCO5122 (10A4)_PCR/210 [Gene]SCO5122/188 NC_003888 SC9E12.07c, probable peptidase, len: 707 aa; similar to TR:P95782 (EMBL:D83263) Xanthomonas maltophilia dipeptidyl peptidase IV, 741 aa; fasta scores: opt: 442 z-score: 478.1 E(): 3.7e-19; 33.2% identity in 733 aa overlap. Contains Pfam matches to entries PF00930 DPPIV_N_term, Dipeptidyl peptidase IV (DPP IV) N-terminal region and PF00326 Peptidase_S9, Prolyl oligopeptidase family and match to Prosite entry PS00708 Prolyl endopeptidase family serine active site 1 1 10 12 [Reporter]SCO2694 (10M24)_PCR/209 [Gene]SCO2694/187 NC_003888 SCC61A.15, unknown, len: 180aa; 1 1 10 11 [Reporter]SCO4437 (11I24)_PCR/208 [Gene]SCO4437/186 NC_003888 SCD6.15c, possible sodium-coupled permease, len: 527 aa; similar to TR:P74252 (EMBL:D90913) Synechocystis sp. sodium-coupled permease, 512 aa; fasta scores: opt: 451 z-score: 531.6 E(): 3.8e-22; 26.3% identity in 510 aa overlap. Contains Pfam match to entry PF00474 SSF, Sodium:solute symporter family and possible hydrophobic membrane spanning regions 1 1 10 10 [Reporter]SCO3507 (12E24)_PCR/207 [Gene]SCO3507/185 NC_003888 SCE134.08, possible integral membrane efflux protein, len: 500aa; similar to many mostly involved in antibiotic efflux eg. TR:Q50396 (EMBL:U46844) from the ethambutol resistance gene cluster of Mycobacterium tuberculosis (412 aa) fasta scores; opt: 780, z-score: 854.0, E(): 0, (36.7% identity in 401 aa overlap). Contains several possible membrane spanning hydrophobic regions. 1 1 10 9 [Reporter]SCO1190 (13A24)_PCR/206 [Gene]SCO1190/184 NC_003888 SCG11A.21c, probable export protein, len: 533 aa; similar to SW:TCMA_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C resistance and export protein TcmA, 538 aa; fasta scores: opt: 1520 z-score: 1545.7 E(): 0; 45.7% identity in 523 aa overlap 1 1 10 8 [Reporter]SCO1343 (14M20)_PCR/205 [Gene]SCO1343/183 NC_003888 2SCG61.25c, ung, uracil-DNA glycosylase (EC 3.2.2.-), len: 227aa; strongly similar to many eg. SW:P12295 (UNG_ECOLI) uracil-DNA glycosylase from Escherichia coli (228 aa) fasta scores; opt: 774, z-score: 935.8, E(): 0, 52.7% identity in 220 aa overlap. Contains Pfam match to entry PF00315 UNG, Uracil-DNA glycosylase and Prosite match to PS00130 Uracil-DNA glycosylase signature. 1 1 10 7 [Reporter]SCO0149 (15I20)_PCR/204 [Gene]SCO0149/182 NC_003888 SCJ33.13c, unknown, len: 198 aa. 1 1 10 6 [Reporter]SCO3306 (16E20)_PCR/203 [Gene]SCO3306/181 NC_003888 SCE68.04c, possible aminotransferase, len: 404 aa; similar to TR:Q56232 (EMBL:D38459), aspC, Thermus aquaticus aspartate aminotransferase (385 aa), fasta scores; opt: 379 z-score: 433.6 E(): 7.9e-17, 28.9% identity in 370 aa overlap and to many hypothetical aminotransferases. Similar to many from S.coelicolor e.g. SCH10.36 (EMBL:AL049754) probable aspartate aminotransferase (396 aa) (33.2% identity in 404 aa overlap). Alternative start codons are present at codons 3 and 7. Contains Pfam match to entry PF00155 aminotran_1, Aminotransferases class-I 1 1 10 5 [Reporter]SCO3314 (17A20)_PCR/202 [Gene]SCO3314/180 NC_003888 SCE68.12c, possible dehydrogenase, len: 318 aa; weakly similar to SW:QOR_CAVPO (EMBL:M26936) Cavia porcellus quinone oxidoreductase (329 aa), fasta scores; opt: 301 z-score: 320.4 E(): 1.6e-10, 27.6% identity in 326 aa overlap. Similar to TR:Q53927 (EMBL:X62373) ORF2 from S.coelicolor actVI locus (329 aa) (33.7% identity in 329 aa overlap). Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases 2 4 15 11 [Reporter]SCO4492 (12N1)_PCR/6869 [Gene]SCO4492/6259 NC_003888 SCD69.12, probable octaprenyl carboxylase, len: 216 aa; similar to TR:P72743 (EMBL:D90900) Synechocystis sp. 3-octaprenyl-4-hydroxybenzoate carboxy-lyase UbiX, 206 aa; fasta scores: opt: 446 z-score: 524.1 E(): 9.9e-22; 40.3% identity in 211 aa overlap and to SW:UBIX_ECOLI (EMBL:M68935) Escherichia coli 3-octaprenyl-4-hydroxybenzoate carboxy-lyase UbiX, 189 aa; fasta scores: opt: 306 z-score: 363.7 E(): 8.5e-13; 38.2% identity in 207 aa overlap 2 4 15 10 [Reporter]SCO3019 (13J1)_PCR/6868 [Gene]SCO3019/6258 NC_003888 SCE33.21, possible lipoprotein, len: 436 aa; similar to TR:O69661 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical 47.7 kD protein MTV025.041, 440 aa; fasta scores: opt: 875 z-score: 982.3 E(): 0; 56.3% identity in 448 aa overlap. Contains Pfam match to entry PF01882 DUF58, Protein of unknown function and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site also contains possible N-terminal region signal peptide sequence 2 4 15 9 [Reporter]SCO3476 (14F1)_PCR/6867 [Gene]SCO3476/6257 NC_003888 SCE65.12c, probable dehydrogenase, len: 251 aa; similar to SW:KDUD_BACSU (EMBL:L47838) Bacillus subtilis 2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125) KduD, 254 aa; fasta scores: opt: 833 z-score: 937.8 E(): 0; 52.0% identity in 248 aa overlap and to Streptomyces coelicolor SCI30A.02, 253 aa; fasta scores: opt: 605 z-score: 622.2 E(): 2.7e-29; 44.1% identity in 245 aa overlap. Contains Pfam matches to entry PF00106 adh_short, short chain dehydrogenase and to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 2 4 15 8 [Reporter]SCO0414 (15B1)_PCR/6866 [Gene]SCO0414/6256 NC_003888 SCF51.13c, possible RNA polymerase sigma factor, len: 298 aa; similar to TR:CAB42013 (EMBL:AL049754) Streptomyces coelicolor putative RNA polymerase sigma factor SCH10.4c, 294aa; fasta scores: opt: 614 z-score: 721.0 E(): 8.7e-33; 41.4% identity in 285 aa overlap and to TR:O86856 (EMBL:AJ007313) Streptomyces coelicolor sigma factor, SigT, 236aa; fasta scores: opt: 160 z-score: 194.6 E(): 0.0018; 31.0% identity in 158 aa overlap 2 4 15 7 [Reporter]SCO3462 (15N21)_PCR/6865 [Gene]SCO3462/6255 NC_003888 SCE46.19, unknown, len: 478 aa 2 4 15 6 [Reporter]SCO0262 (16J21)_PCR/6864 [Gene]SCO0262/6254 NC_003888 SCF1.04, probable LysR-family transcriptional regulator, len: 311 aa; similar to many transcriptional regulators especially in the N-terminal region, e.g. SW:OXYR_MYCAV regulator of hydrogen peroxide responsive genes from Mycobacterium avium (311 aa) fasta scores; opt: 292, z-score: 329.4, E(): 5.5e-11, (36.6% identity in 191 aa overlap). Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and Prosite match to PS00044 Bacterial regulatory proteins, lysR family signature. 2 4 15 5 [Reporter]SCO5526 (17F21)_PCR/6863 [Gene]SCO5526/6253 NC_003888 SC1C2.07, ureC, probable urease alpha subunit, len: 558 aa; similar to many e.g. URE1_YEREN urease alpha subunit (EC 3.5.1.5) (571 aa), fasta scores; opt: 1676 z-score: 1972.3 E(): 0, 45.2% identity in 564 aa overlap. Contains PS01120 Urease nickel ligands signature and Pfam match to entry PF00449 urease, Urease, score 758.30, E-value 3.3e-224 2 4 15 4 [Reporter]SCO3429 (18B21)_PCR/6862 [Gene]SCO3429/6252 NC_003888 SCE9.36, rpmB, probable 50S ribosomal protein L28, len: 73 aa; similar to many e.g. SW:RL28_ECOLI (EMBL:J01677), rpmB, Escherichia coli 50S ribosomal protein L28 (77 aa), fasta scores; opt: 235 z-score: 344.6 E(): 7.1e-12, 47.8% identity in 69 aa overlap. Contains Pfam match to entry PF00830 Ribosomal_L28, Ribosomal L28 family, score 88.70, E-value 1.2e-22 1 3 21 5 [Reporter]SCO3716 (18M2)_PCR/4476 [Gene]SCO3716/4079 NC_003888 SCH35.08, probable cation transport system component, len: 225 aa; similar to many e.g. SW:ATKc_ECOLI AtkC/KdpC potassium transporting ATPase A chain from Escherichia coli (190 aa) fasta scores; opt: 230, z-score: 264.8, E(): 2e-07, (35.0% identity in 203 aa overlap). Contains possible membrane spanning hydrophobic region 2 4 15 3 [Reporter]SCO4034 (19N17)_PCR/6861 [Gene]SCO4034/6251 NC_003888 2SC10A7.38c, probable RNA polymerase sigma factor, len: 318 aa; highly similar to TR:Q9RIS5 (EMBL:AJ249581) Streptomyces coelicolor A3(2) Sig15 protein, 304 aa; fasta scores: opt: 1201 z-score: 1341.5 E(): 0; 66.6% identity in 287 aa overlap and to SW:RPOF_STRCO (EMBL:L11648) Streptomyces coelicolor RNA polymerase sigma-F factor SigF or RpoX, 287 aa; fasta scores: opt: 950 z-score: 1063.4 E(): 0; 58.6% identity in 261 aa overlap. Contains Pfam match to entry PF00140 sigma70, Sigma-70 factor and match to Prosite entry PS00715 Sigma-70 factors family signature 1. Also contains a possible helix-turn-helix motif at residues 284..305 (+6.30 SD) 1 3 21 4 [Reporter]SCO5336 (19I2)_PCR/4475 [Gene]SCO5336/4078 NC_003888 SCBAC5H2.05c, possible mutT-like protein, len: 156 aa; similar to plasmid borne SW:MUTT_STRAM (EMBL:Z19590) Streptomyces ambofaciens plasmid pSAM2 mutT-like protein ORF154, 154 aa; fasta scores: opt: 182 z-score: 232.1 E(): 2.4e-05; 32.7% identity in 153 aa overlap. Contains Pfam match to entry PF00293 mutT, MutT-like domain, and match to Prosite entry PS00893 mutT domain signature 2 4 15 2 [Reporter]SCO5995 (20F17)_PCR/6860 [Gene]SCO5995/6250 NC_003888 SCBAC16H6.30, possible secreted protein, len: 240aa: similar to TR:Q9KYR3 (EMBL:AL355913) putative lipoprotein SC5H4.26c from Streptomyces coelicolor (187 aa) fasta scores; opt: 133, Z-score: 148.7, 31.783% identity (36.607% ungapped) in 129 aa overlap. Contains a possible N-terminal signal sequence. Also contains TTA leucine codon. Possible target for bldA regulation. 1 3 21 3 [Reporter]SCO6103 (20E2)_PCR/4474 [Gene]SCO6103/4077 NC_003888 SCBAC1A6.27c, possible acetyltransferase, len: 190 aa. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 3 21 2 [Reporter]SCO0975 (20M22)_PCR/4473 [Gene]SCO0975/4076 NC_003888 SCBAC19F3.02, zwf3, 6-phosphogluconate 1-dehydrogenase, len: 479 aa: similar to many eg. SW:P00350 (6PGD_ECOLI) 6-phosphogluconate dehydrogenase from Escherichia coli (468 aa) fasta scores; opt: 1627, Z-score: 1751.0, 53.433% identity (54.013% ungapped) in 466 aa overlap. Contains Pfam match to entry PF00393 6PGD, 6-phosphogluconate dehydrogenase. 1 3 20 22 [Reporter]SCO1340 (1M18)_PCR/4471 [Gene]SCO1340/4075 NC_003888 2SCG61.22, conserved hypothetical protein, len: 170 aa; similar to others of unknown function from Streptomyces coelicolor e.g. TR:CAA20815 (EMBL:AL031541) hypothetical protein (175 aa) fasta scores; opt: 568, z-score: 738.0, E(): 0, 52.1% identity in 169 aa overlap, to SW:YM2_STRCO (EMBL:X15942) mini-circle hypothetical 13.3 kD protein (122 aa) fasta scores; opt: 366, z-score: 482.1, E(): 2.2e-19, 47.2% identity in 123 aa overlap and to TR:Q9AJE6 (EMBL:AB048795) Kitasatospora griseola hypothetical protein, 170 aa; fasta scores: opt: 614 Z-score: 754.9 E(): 2.1e-34; 56.140% identity in 171 aa overlap 1 3 20 21 [Reporter]SCO4958 (2I18)_PCR/4470 [Gene]SCO4958/4074 NC_003888 2SCK31.18, metB, cystathionine gamma-synthase, len: 386 aa; highly similar to TR:Q9RQ97 (EMBL:AF126953) Corynebacterium glutamicum cystathionine gamma-synthase MetB, 396 aa; fasta scores: opt: 1497 z-score: 1715.4 E(): 0; 59.2% identity in 377 aa overlap. Contains Pfam match to entry PF01053 Cys_Met_Meta_PP, Cys/Met metabolism PLP-dependent enzyme and match to Prosite entry PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1 1 3 20 20 [Reporter]SCO7649 (3E18)_PCR/4469 [Gene]SCO7649/4073 NC_003888 SC10F4.22c, probable two-component system sensor kinase, len: 398 aa; similar to many other Streptomyces coelicolor sensor kinases, e.g. TR:Q9RKT9 (EMBL:AL132824) putative two-component sensor kinase SCAH10.18, 417 aa; fasta scores: opt: 780 z-score: 815.5 E(): 0; 44.0% identity in 348 aa overlap. Contains possible hydrophobic membrane spanning regions in the N-terminal domain 1 3 20 19 [Reporter]SCO5603 (4A18)_PCR/4468 [Gene]SCO5603/4072 NC_003888 SC2E1.20, unknown ATP/GTP binding protein, len: 541 aa; similar to hypothetical proteins from many organisms e.g. YIFB_ECOLI (516 aa), fasta scores; opt: 356 z-score: 935.9 E(): 0, 37.0% identity in 513 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 20 18 [Reporter]SCO7676 (5M14)_PCR/4467 [Gene]SCO7676/4071 NC_003888 SC4C2.11, probable ferredoxin, len: 73 aa; similar to SW:FER2_STRGO (EMBL:M32239) Streptomyces griseolus ferredoxin 2 SubB, 64 aa; fasta scores: opt: 236 z-score: 326.6 E(): 1.2e-10; 55.7% identity in 61 aa overlap 1 1 10 4 [Reporter]SCP1.201 (18M16)_PCR/201 [Gene]SCP1.201/179 NC_003888 SCP1.201, unknown, len: 1336aa; similar to TR:O87848 (EMBL:AL031013) hypothetical protein from Streptomyces coelicolor (1147 aa) fasta scores; opt: 1652, z-score: 1478.4, E(): 0, 38.7% identity in 926 aa overlap. Note possible alternative upstream translational start site which would give a possible N-terminal signal sequence. Contains several possible coiled-coil regions. 1 3 20 17 [Reporter]SCO6746 (6I14)_PCR/4466 [Gene]SCO6746/4070 NC_003888 SC5F2A.29, possible transcriptional regulator, len: 325aa; similar to many eg. TR:O30507 (EMBL:AF008308) arginine regulatory protein ArgR, from Pseudomonas aeruginosa (328 aa) fasta scores; opt: 288, z-score: 331.0, E(): 4e-11, (24.7% identity in 308 aa overlap). Also similar to TR:Q43970 (EMBL:Z48959) AraC-like protein from Azorhizobium caulinodans (227 aa) fasta scores; opt: 605, z-score: 689.7, E(): 4.2e-31, (47.4% identity in 192 aa overlap). Contains Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. 1 1 10 3 [Reporter]SCO4902 (19I16)_PCR/200 [Gene]SCO4902/178 NC_003888 2SCK8.28, possible secreted protein, len: 122 aa. Contains possible cleavable N-terminal region signal peptide sequence 1 1 10 2 [Reporter]SCO3057 (20A16)_PCR/199 [Gene]SCO3057/177 NC_003888 SCBAC19G2.12c, possible dipeptidase, len: 376 aa: similar to many eukaryotic peptidases e.g. SW:P31430 (MDP1_RAT) rat renal dipeptidase (410 aa) fasta scores; opt: 440, Z-score: 474.0, 36.056% identity (38.906% ungapped) in 355 aa overlap. Also weakly similar to TR:Q9KH70 (EMBL:AF268476) thermostable dipeptidase from Brevibacillus borstelensis (307 aa) fasta scores; opt: 273, Z-score: 298.4, 28.159% identity (30.116% ungapped) in 277 aa overlap. Contains Pfam match to entry PF01244 Renal_dipeptase, Renal dipeptidase. Also similar to neighbouring upstream CDS SCBAC19G2.13c 1 1 9 22 [Reporter]SCO2803 (1A12)_PCR/197 [Gene]SCO2803/176 NC_003888 2SCC13.11c, hypothetical protein, len: 289 aa; similar to TR:CAB95777 (EMBL:AL359949) Streptomyces coelicolor hypothetical 29.6 kDa protein 2SCG61.02c, 281 aa; fasta scores: opt: 1064 z-score: 1175.8 E(): 0; 61.4% identity in 267 aa overlap 1 1 9 20 [Reporter]SCO1505 (3I8)_PCR/195 [Gene]SCO1505/175 NC_003888 SC9C5.29c, rspD, 30S ribosomal protein S4, len: 204 aa; highly similar to SW:RS4_BACST Bacillus stearothermophilus 30S ribosomal protein S4 RspD, 198 aa; fasta scores: opt: 531 z-score: 651.1 E(): 8.6e-29; 42.9% identity in 198 aa overlap. Contains Pfam matches to entries PF00163 Ribosomal_S4, Ribosomal protein S4/S9 N-terminal domain and PF01479 S4, S4 domain 1 1 9 19 [Reporter]SCO5530 (4E8)_PCR/194 [Gene]SCO5530/174 NC_003888 SC1C2.11, putative membrane protein, len: 450 aa; contains probable hydrophobic membrane spanning regions and highly Gly-rich C-terminus 1 1 9 18 [Reporter]SCO6382 (5A8)_PCR/193 [Gene]SCO6382/173 NC_003888 SC3C8.01, possible secreted protein, len: 302 aa; some similarity to M. tuberculosis putative lipoprotein YX36_MYCTU MTCY274.36 (343 aa), fasta scores; opt: 201 z-score:255.1 E(): 5.7e-07, 24.9% identity in 257 aa overlap. Contains possible N-terminal signal sequence 1 1 9 17 [Reporter]SCO6675 (6M4)_PCR/192 [Gene]SCO6675/172 NC_003888 SC5A7.25c, possible DNA-binding protein, len: 218 aa. Contains probable helix-turn-helix motif from aa 92-113 (Score 1665, +4.86 SD) 1 1 9 16 [Reporter]SCO3618 (7I4)_PCR/191 [Gene]SCO3618/171 NC_003888 SC66T3.29c, recR, probable recomination protein, len: 199 aa; highly similar to many e.g. SW:RECR_BACSU (EMBL:X17014), recR, Bacillus subtilis recomination protein (198 aa), fasta scores; opt: 794 z-score: 939.4 E(): 0, 56.6% identity in 196 aa overlap 1 1 9 15 [Reporter]SCO6690 (8E4)_PCR/190 [Gene]SCO6690/170 NC_003888 SC6G3.06c, probable transmembrane transport protein, len: 426 aa; similar to many transporters e.g. SW:KGTP_ECOLI (EMBL:X53027), kgtP, Escherichia coli alpha-ketoglutarate permease (432 aa), fasta scores; opt: 1088 z-score: 1249.1 E(): 0, 41.1% identity in 419 aa overlap. Highly similar to TR:Q03095 (EMBL:M64783) Streptomyces hygroscopicus putative bialaphos transport protein (447 aa) (57.3% identity in 412 aa overlap). Similar to TR:O86563 (EMBL:AL031184) S.coelicolor probable transmembrane transport protein (472 aa) (31.7% identity in 436 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to PF00083 sugar_tr, Sugar (and other) transporter, PS00216 Sugar transport proteins signature 1 and PS00217 Sugar transport proteins signature 2 2 4 14 22 [Reporter]SCO1283 (1F13)_PCR/6858 [Gene]SCO1283/6249 NC_003888 2SCG18.30c, hypothetical protein, len: 155 aa; low similarity to N-terminal region of TR:Q53669 (EMBL:X89899) Streptomyces argillaceus mithramycin poliketide synthase MtmQ, 315 aa; fasta scores: opt: 130 z-score: 171.4 E(): 0.046; 27.5% identity in 120 aa overlap 2 4 14 21 [Reporter]SCO5627 (2B13)_PCR/6857 [Gene]SCO5627/6248 NC_003888 SC6A9.40c, frr, ribosome recycling factor, len: 185 aa; highly similar to many e.g. RRF_ECOLI ribosome recycling factor (ribosome releasing factor) (185 aa), fasta scores; opt: 504 z-score: 841.1 E(): 0, 41.8% identity in 177 aa overlap 2 4 14 20 [Reporter]SCO5899 (3N9)_PCR/6856 [Gene]SCO5899/6247 NC_003888 SC10A5.04, unknown, len: 295 aa; similar to hypothetical proteins from Mycobacterioum tuberculosis and M. leprae e.g. YV29_MYCTU Q11167 hypothetical 39.3 kd protein CY20G9.29 (358 aa), fasta scores; opt: 1075 z-score: 1514.7 E(): 0, 57.8% identity in 263 aa overlap 2 4 14 19 [Reporter]SCO6937 (4J9)_PCR/6855 [Gene]SCO6937/6246 NC_003888 SC1G8.09c, possible DNA-binding protein, len: 186 aa. Contains a possible helix-turn-helix motif situated between residues 54..75 (+4.70 SD). 2 4 14 18 [Reporter]SCO6404 (5F9)_PCR/6854 [Gene]SCO6404/6245 NC_003888 SC3C8.23, unknown, len: 59 aa 2 4 14 17 [Reporter]SCO2066 (6B9)_PCR/6853 [Gene]SCO2066/6244 NC_003888 SC4G6.35, possible membrane protein, len: 236aa; Contains possible membrane spanning hydrophobic regions. 2 4 14 15 [Reporter]SCO7243 (8J5)_PCR/6851 [Gene]SCO7243/6243 NC_003888 SC7A12.10c, possible secreted protein, len: 597 aa. Similar to Streptomyces coelicolor TR:Q9RKP5(EMBL:AL133220) possible secreted esterase, SCC75A.29C (351 aa), fasta scores opt: 955 z-score: 1080.4 E():0 53.5% identity in 357 aa overlap. Contains a possible N-terminal signal sequence 2 4 14 14 [Reporter]SCO7725 (9F5)_PCR/6850 [Gene]SCO7725/6242 NC_003888 SC8D11.16, unknown, len: 260 aa. High content in alanine amino acid residues 1 3 20 16 [Reporter]SCO5633 (7E14)_PCR/4465 [Gene]SCO5633/4069 NC_003888 SC6A9.34, probable fusion protein partially within putative integrated plasmid, len: 259 aa; N-terminus is almost identical to TR:E1286466 (EMBL:AJ005260) plasmid transfer protein traSA from Streptomyces ambofaciens plasmid pSAM2 (415 aa); 96.2% identity in 369 aa overlap, and similar to other members of the spoIIIE /ftsK family e.g. SPI_STRAZ sporulation-regulatory protein (303 aa), fasta scores; opt: 608 z-score: 904.0 E(): 0, 57.0% identity in 235 aa overlap. C-terminus is similar to many integrase /recombinase proteins e.g. TR:O55248 (EMBL:AF030986) Mycobacterium phage MS6 integrase (372 aa), fasta scores; opt: 184 z-score: 323.4 E(): 8.9e-11, 26.4% identity in 364 aa overlap. N-terminus contains TTA Leu codon; possible target for action of bldA and PS00017 ATP /GTP-binding= Pfam match to entry PF00589 Phage_integrase, Phage integrase family, score 40.60, E-value 3.5e-08 2 4 14 13 [Reporter]SCO5131 (10B5)_PCR/6849 [Gene]SCO5131/6241 NC_003888 SC9E12.16, possible two-component system sensor kinase, len: 388 aa; similar to TR:CAB88185 (EMBL:AL352972) Streptomyces coelicolor putative two-component system sensor kinase SCC30.15, 409 aa; fasta scores: opt: 773 z-score: 840.8 E(): 0; 41.9% identity in 389 aa overlap. Contains possible hydrophobic membrane spanning regions at N-terminal domain 1 3 20 15 [Reporter]SCO5214 (8A14)_PCR/4464 [Gene]SCO5214/4068 NC_003888 SC7E4.11, hypothetical protein, len: 271 aa; similar to TR:O05872 (EMBL:Z95121) Mycobacterium tuberculosis hypothetical 27.6 kD protein MTCY20B11.01c, 252 aa; fasta scores: opt: 383 z-score: 460.8 E(): 3.4e-18; 39.6% identity in 283 aa overlap 2 4 14 12 [Reporter]SCO2350 (11N1)_PCR/6848 [Gene]SCO2350/6240 NC_003888 SCC8A.08, possible integral membrane protein, len: 153 aa; similar to TR:O86323 (EMBL:Z83866) hypothetical protein from Mycobacterium tuberculosis (141 aa) fasta scores; opt: 391, z-score: 477.0, E(): 4.2e-19, 48.5% identity in 130 aa overlap, to TR:CAB85407 (EMBL:AL162758) conserved inner membrane hypothetical protein from Neisseria meningitidis (132 aa) fasta scores; opt: 328, z-score: 402.9, E(): 5.7e-15, 41.9% identity in 129 aa overlap and to TR:Q9K461 (EMBL:AL359215) Streptomyces coelicolor putative integral membrane protein SC2H12.23c, 151 aa; fasta scores: opt: 533 Z-score: 626.7 E(): 2.8e-27; 58.741% identity in 143 aa overlap. Contains possible membrane-spanning hydrophobic regions. 1 3 20 14 [Reporter]SCO7004 (9M10)_PCR/4463 [Gene]SCO7004/4067 NC_003888 SC8F11.30, probable carbohydrate kinase,len: 479 aa. Highly similar to many e.g. Bacillus subtilis SW:GLPK_BACSU(EMBL:M34393) glycerol kinase (EC 2.7.1.30), GlpK (496 aa), fasta scores opt: 688 z-score: 766.4 E():0 35.1% identity in 496 aa overlap. Contains a Pfam match to entry PF00370 FGGY, FGGY family of carbohydrate kinases. 1 3 20 13 [Reporter]SCO2566 (10I10)_PCR/4462 [Gene]SCO2566/4066 NC_003888 SCC123.04c, hypothetical protein, len: 293 aa. Similar in parts to several proteins of unknown function e.g. Streptomyces coelicolor TR:O86515(EMBL:AL031124) hypothetical 20.0 KD protein (185 aa), fasta scores: opt: 384 z-score: 456.7 E(): 4.7e-18 32.8% identity in 180 aa overlap 1 3 20 12 [Reporter]SCO2624 (11E10)_PCR/4461 [Gene]SCO2624/4065 NC_003888 SCC80.09c, possible integral membrane protein, len: 395 aa; similar to TR:Q9RL36 (EMBL:AL121596) Streptomyces coelicolor hypothetical 29.6 kDa protein SCF51A.29c, 284 aa; fasta scores: opt: 269 z-score: 309.0 E(): 1.1e-09; 31.0% identity in 258 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 20 11 [Reporter]SCO4481 (12A10)_PCR/4460 [Gene]SCO4481/4064 NC_003888 SCD69.01, probable serine/threonine protein kinase (fragment), len: >607 aa; similar to SW:AFSK_STRCO (EMBL:D15062) Streptomyces coelicolor serine/threonine protein kinase AfsK, 799 aa; fasta scores: opt: 649 z-score: 427.0 E(): 2.5e-16; 35.8% identity in 408 aa overlap also similar to S. coelicolor SCD69.08 and TR:Q9ZN80 (EMBL:AB021679) Streptomyces coelicolor serine/threonine protein kinase PkaG, 592 aa; fasta scores: opt: 715 z-score: 417.5 E(): 1e-17; 32.7% identity in 538 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and matches to Prosite entries PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature. Contains also a TTA leucine codon, possible target for bldA regulation,SCD65.24, probable serine/threonine protein kinase (fragment), len: >56 aa; similar to SW:AFSK_STRCO (EMBL:D15062) Streptomyces coelicolor serine/threonine protein kinase AfsK (EC 2.7.1.-), 799 aa; fasta scores: opt: 137 z-score: 184.3 E(): 0.0092; 52.4% identity in 42 aa overlap and to TR:CAB92109 (EMBL:AL356334) Streptomyces coelicolor putative serine/threonine protein kinase SCD69.08, 626 aa; fasta scores: opt: 316 z-score: 370.1 E(): 5.6e-15; 86.8% identity in 53 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and match to Prosite entry PS00107 Protein kinases ATP-binding region signature 1 3 20 10 [Reporter]SCO3273 (13M6)_PCR/4459 [Gene]SCO3273/4063 NC_003888 SCE39.23, unknown, len: 88aa; contains possible coiled-coil. 1 3 20 9 [Reporter]SCO3909 (14I6)_PCR/4458 [Gene]SCO3909/4062 NC_003888 SCH24.31, rplI, 50S ribosomal protein L9, len: 148 aa; similar to many e.g. SW:RL9_BACST (EMBL:X62002), RplI, Bacillus stearothermophilus 50S ribosomal protein L9 (149 aa), fasta scores; opt: 346 z-score: 415.7 E(): 7.7e-16, 36.6% identity in 145 aa overlap. Contains Pfam match to entry PF01281 Ribosomal_L9, Ribosomal protein L9, score 203.80, E-value 2.6e-57 and PS00651 Ribosomal protein L9 signature 1 3 20 8 [Reporter]SCO1912 (15E6)_PCR/4457 [Gene]SCO1912/4061 NC_003888 SCI7.30, probable dihydrodipicolinate synthase, len: 317 aa; similar to many egs. TR:O86841 (EMBL:AL031260) putative dihydrodipicolinate synthase from Streptomyces coelicolor (299 aa) fasta scores; opt: 1018, z-score: 1116.9, E(): 0, (56.4% identity in 305 aa overlap) and SW:DAPA_BRELA dihydrodipicolinate synthase from Brevibacterium lactofermentum (301 aa) fasta scores; opt: 800, z-score: 879.6, E(): 0, (46.6% identity in 292 aa overlap). Contains Pfam match to entry PF00701 DHDPS, Dihydrodipicolinate synthetase family and Prosite matches to PS00665 Dihydrodipicolinate synthetase signature 1 and PS00666 Dihydrodipicolinate synthetase signature 2. 1 1 9 14 [Reporter]SCO7712 (9A4)_PCR/189 [Gene]SCO7712/169 NC_003888 SC8D11.03, possible two component system response regulator, len: 226 aa; similar to TR:Q53894 (EMBL:U51332) Streptomyces coelicolor AbsA2, 222 aa; fasta scores: opt: 763 z-score: 842.6 E(): 0; 55.3% identity in 219 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Also contains possible helix-turn-helix motif at residues 174..195 (+3.33 SD) 1 3 20 7 [Reporter]SCO3865 (16A6)_PCR/4456 [Gene]SCO3865/4060 NC_003888 SCH18.02, hypothetical protein, len: 260 aa; similar to TR:CAB72374 (EMBL:AL138977) Streptomyces coelicolor hypothetical 27.3 kD protein SC7F9.21c, 247 aa; fasta scores: opt: 296 z-score: 343.9 E(): 1.1e-11; 32.9% identity in 234 aa overlap. Contains and match to Prosite entry 1 1 9 13 [Reporter]SCO6470 (9M24)_PCR/188 [Gene]SCO6470/168 NC_003888 SC9C7.06c, unknown, len: 170 aa; similar to TR:O06163 (EMBL:Z95556) from Mycobacterium tuberculosis (185 aa) fasta scores; opt: 342, z-score: 497.2, E(): 2.1e-20, (38.8% identity in 152 aa overlap). 1 1 9 12 [Reporter]SCO2690 (10I24)_PCR/187 [Gene]SCO2690/167 NC_003888 SCC61A.11, possible membrane transport protein, len: 454aa; similar to eg. TR:Q54309 (EMBL:X86780) membrane transport protein from rapamycin biosynthetic gene cluster of Streptomyces hygroscopicus (453 aa) fasta scores; opt: 546, z-score: 566.6, E(): 4.2e-24, 27.7% identity in 452 aa overlap. Contains possible membrane-spanning hydrophobic regions. 1 1 9 11 [Reporter]SCO4687 (11E24)_PCR/186 [Gene]SCO4687/166 NC_003888 SCD31.12c, unknown, len: 119 aa 1 1 9 10 [Reporter]SCO3503 (12A24)_PCR/185 [Gene]SCO3503/165 NC_003888 SCE134.04c, possible binding protein dependent transport protein, len: 279aa; similar to many egs. SW:YURM_BACSU hypothetical ABC transporter permease from Bacillus subtilis (300 aa) fasta scores; opt: 424, z-score: 513.6, E(): 2.7e-21, (32.4% identity in 281 aa overlap) and SW:UGPE_ECOLI Sn-glycerol-3-phosphate transport permease from Escherichia coli (281 aa) fasta scores; opt: 350, z-score: 425.7, E(): 2.1e-16, (29.6% identity in 284 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. 1 1 9 9 [Reporter]SCO3577 (13M20)_PCR/184 [Gene]SCO3577/164 NC_003888 SCH17.11, possible ion-transporting ATPase, len: 325 aa; similar to N-terminus of TR:O50593 (EMBL:AB004659), arsA, ATPase subunit of Acidiphilium multivorum arsenite transport protein (583 aa), fasta scores; opt: 204 z-score: 231.6 E(): 1.4e-05, 25.8% identity in 325 aa overlap. Also similar to TR:O69647 (EMBL:AL022121) Mycobacterium tuberculosis putative anion transporting ATPase (340 aa) (52.1% identity in 328 aa overlap). Part of the N-terminus is weakly similar to the adjacent CDS SCH17.12 (481 aa) (25.9% identity in 158 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 1 9 7 [Reporter]SCO0963 (15E20)_PCR/182 [Gene]SCO0963/163 NC_003888 SCM11.18c, hypothetical protein, len: 297 aa; C-terminal region identical to previously sequenced TR:Q9X9U6 (EMBL:AJ243803) Streptomyces coelicolor hypothetical 18.7 kD protein (fragment), 181 aa. Contains proline-rich C-terminal region 1 1 9 6 [Reporter]SCO0208 (16A20)_PCR/181 [Gene]SCO0208/162 NC_003888 SCJ12.20, pyruvate phosphate dikinase, len: 898 aa. Highly similar to many pyruvate phosphate dikinases e.g. Clostridium symbiosum SW:PODK_CLOSY (EMBL; M60920)(EC 2.7.9.1) (873 aa), fasta scores opt: 1626 z-score: 1761.3 E():0 48.7% identity in 895 aa overlap and Microbispora rosea SW:BAA76347 (EMBL; AB025020) (878 aa), fasta scores opt: 3060 z-score: 3317.5 E(): 0 67.5% identity in 898 aa overlap. Contains a PS00370 PEP-utilizing enzymes phosphorylation site signature, Pfam match to entry PF01326 PPDK_N_term, Pyruvate phosphate dikinase, PEP/pyruvate binding domain and a Pfam match to entry PF00391 PEP-utilizers, PEP-utilizing enzymes 1 1 9 5 [Reporter]SCO1053 (17M16)_PCR/180 [Gene]SCO1053/161 NC_003888 SCG20A.33c, unknown, len: 142. Doubtful CDS 1 1 9 4 [Reporter]SCO1979 (18I16)_PCR/179 [Gene]SCO1979/160 NC_003888 SC3C9.14c, probable DNA-binding protein, len: 286 aa; similar to many Streptomyces DNA-binding proteins e.g. TR:Q9F3E2 (EMBL:AL450289) Streptomyces coelicolor putative DNA-binding proteins SC2H2.13, 290 aa; fasta scores: opt: 1019 Z-score: 1178.0 bits: 225.9 E(): 5.2e-58; 55.000% identity in 280 aa overlap and TR:O86719 (EMBL:AL031515) SC5C7.22c, 287 aa; fasta scores: opt: 1360 Z-score: 1390.2 bits: 265.1 E(): 1e-71; 69.338% identity in 287 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and a possible helix-turn-helix motif at residues 28..49 (+4.23 SD) 2 4 14 10 [Reporter]SCO2855 (13F1)_PCR/6846 [Gene]SCO2855/6239 NC_003888 SCE20.29, unknown, len: 338 aa. 2 4 14 9 [Reporter]SCO0109 (14B1)_PCR/6845 [Gene]SCO0109/6238 NC_003888 SCJ11.38c, unknown, len: 156aa; shows similarity to human proteins involved in the expression of GAGE genes eg. TR:AAC33676 (EMBL:AF055473) GAGE-7 from Homo sapiens (Human) (116 aa) fasta scores; opt: 114, z-score: 144.7, E(): 1.1, (31.9% identity in 119 aa overlap). 2 4 14 8 [Reporter]SCO4722 (14N21)_PCR/6844 [Gene]SCO4722/6237 NC_003888 SCD31.47, secY, preprotein translocase SecY subunit, len: 437 aa; identical to previously sequenced SW:SECY_STRCO (EMBL:X83011) Streptomyces coelicolor preprotein translocase SecY subunit, 437 aa. Contains Pfam match to entry PF00344 secY, eubacterial secY protein and matches to Prosite entries PS00755 Protein secY signature 1 and PS00756 Protein secY signature 2. Contains also possible hydrophobic membrane spanning regions 2 4 14 7 [Reporter]SCO0648 (15J21)_PCR/6843 [Gene]SCO0648/6236 NC_003888 SCF91.08, possible methyltransferase, len: 231 aa. Similar in parts to several including: Streptomyces avermitilis TR:BAA84602(EMBL:AB032524) C5-O-methyltransferase (283 aa), fasta scores opt: 243 z-score: 292.9 E(): 6.1e-09 34.5% identity in 168 aa overlap and Streptomyces lavendulae TR:AAD28458(EMBL:AF127374) methyltransferase (275 aa), fasta scores opt: 235 z-score: 283.7 E(): 2e-08 32.9% identity in 167 aa overlap. 2 4 14 6 [Reporter]SCO1534 (16F21)_PCR/6842 [Gene]SCO1534/6235 NC_003888 SCL2.24, possible DNA polymerase III, len: 241 aa; similar to SW:DP3E_RICPR (EMBL:AJ235273) Rickettsia prowazekii DNA polymerase III, epsilon chain (EC 2.7.7.7) DnaQ, 229 aa; fasta scores: opt: 200 z-score: 245.9 E(): 2.7e-06; 26.7% identity in 195 aa overlap and to SW:DP3E_ECOLI (EMBL:X04027) Escherichia coli DNA polymerase III, epsilon chain (EC 2.7.7.7) DnaQ, 243 aa; fasta scores: opt: 148 z-score: 184.0 E(): 0.0078; 27.6% identity in 181 aa overlap. Contains Pfam match to entry PF00929 Exonuclease 2 4 14 5 [Reporter]SCO1801 (17B21)_PCR/6841 [Gene]SCO1801/6234 NC_003888 SCI5.09c, possible two component response regulator, len: 210aa; similar to many eg. TR:CAB42027 (EMBL:AL049754) putative two component response regulator from Streptomyces coelicolor (228 aa) fasta scores; opt: 622, z-score: 715.0, E(): 1.7e-32, (48.6% identity in 214 aa overlap) and SW:NARL_ECOLI nitrite/nitrate response regulator from Escherichia coli (216 aa) fasta scores; opt: 512, z-score: 591.0, E(): 1.4e-25, (39.9% identity in 203 aa overlap). Contains Pfam matches to PF00196 Bacterial regulatory proteins, luxR family and PF00072 Response regulator receiver domain, and Prosite match to PS00622 Bacterial regulatory proteins, luxR family signature. Also contains probable helix-turn-helix 165-186aa (+3.23 SD). 2 4 14 4 [Reporter]SCO7516 (18N17)_PCR/6840 [Gene]SCO7516/6233 NC_003888 SCBAC25F8.08, htpG, heat shock protein, len: 638 aa; similar to SW:HTPG_ECOLI (EMBL:M38777) Escherichia coli heat shock protein HtpG, 624 aa; fasta scores: opt: 1304 Z-score: 1419.3 bits: 272.8 E(): 1.9e-71; 46.552% identity in 638 aa overlap. Contains Pfam match to entry PF02518 HATPase_c, Histidine kinase-, DNA gyrase B-, phytochrome-like ATPase and 2x PF00183 HSP90, Hsp90 protein and match to Prosite entry PS00298 Heat shock hsp90 proteins family signature 2 4 14 3 [Reporter]SCP1.218c (19J17)_PCR/6839 [Gene]SCP1.218c/6232 NC_003888 SCP1.218c, unknown, len: 128aa; 1 3 20 6 [Reporter]SCO3791 (17M2)_PCR/4455 [Gene]SCO3791/4059 NC_003888 SCH63.38, hypothetical protein, len: 445 aa; C-terminal region similar to TR:Q9S2I1 (EMBL:AL109732) Streptomyces coelicolor hypothetical protein SC7H2.36c, 252 aa; fsta scores: opt: 620 Z-score: 451.7 E(): 1.6e-17; 45.175% identity in 228 aa overlap 2 4 14 2 [Reporter]SCO7709 (20B17)_PCR/6838 [Gene]SCO7709/6231 NC_003888 SCBAC12C8.10, possible MarR-family transcriptional regulator, len: 142 aa: similar to many e.g. TR:Q9F3P8 (EMBL:AL450350) putative MarR-family regulatory protein SC10F4.12 from Streptomyces coelicolor fasta scores; opt: 337, Z-score: 398.2, 46.923% identity in 130 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family. 1 3 20 5 [Reporter]SCO5024 (18I2)_PCR/4454 [Gene]SCO5024/4058 NC_003888 SCK15.26, possible oxidoreductase, len: 290 aa; similar to TR:Q9UXP0 (EMBL:AJ011519) Methanolobus tindarius F420-dependent N5,N10-methylene-tetrahydromethanopterin reductase, putative FfdA, 326 aa; fasta scores: opt: 323 z-score: 369.8 E(): 4e-13; 28.3% identity in 240 aa overlap 2 4 13 22 [Reporter]SCO1279 (1B13)_PCR/6836 [Gene]SCO1279/6230 NC_003888 2SCG18.26, possible secreted protein, len: 292 aa; similar to TR:O07736 (EMBL:Z97193) Mycobacterium tuberculosis hypothetical 33.3 kD protein MTCY180.22, 303 aa; fasta scores: opt: 420 z-score: 478.9 E(): 3.4e-19; 36.8% identity in 247 aa overlap. Contains possible N-terminal signal peptide sequence 1 3 20 4 [Reporter]SCP1.272c (19E2)_PCR/4453 [Gene]SCP1.272c/4057 NC_003888 SCP1.272c, unknown, doubtful CDS, len: 148aa; 1 3 20 3 [Reporter]SCO1360 (20A2)_PCR/4452 [Gene]SCO1360/4056 NC_003888 SC10A9.02c, probable gntR-family transcriptional regulator, len: 445 aa; similar to SW:PTSJ_SALTY (EMBL:U11243) Salmonella typhimurium putative transcriptional regulatory protein PtsJ, 430 aa; fasta scores: opt: 968 z-score: 1010.8 E(): 0; 40.4% identity in 433 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and possible helix-turn-helix motif at residues 35..56 (+4.36 SD) 1 3 20 2 [Reporter]SCO7490 (20I22)_PCR/4451 [Gene]SCO7490/4055 NC_003888 SCBAC17A6.23, possible oxidoreductase, len: 381aa; similar to many eg. TR:Q9F1Z9 (EMBL:AB033991) BtrE from Bacillus circulans (349 aa) fasta scores: opt: 710, Z-score: 676.3, 38.873% identity (41.071% ungapped) in 355 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases. N-terminus rich in pro/val/ser. 1 3 19 22 [Reporter]SCO1336 (1I18)_PCR/4449 [Gene]SCO1336/4054 NC_003888 2SCG61.18, possible lipoprotein N-acyltransferase (integral membrane), len: 534a; similar to many e.g. SW:P23930 (LNT_ECOLI) apolipoprotein N-acyltransferase (EC 2.3.1.-) from Escherichia coli (512 aa) fasta scores; opt: 391, z-score: 419.0, E(): 7.3e-16, 27.0% identity in 515 aa overlap and TR:O53536 (EMBL:AL021925) hypothetical protein from Mycobacterium tuberculosis (360 aa) fasta scores; opt: 955, z-score: 1020.2, E(): 0, 49.2% identity in 317 aa overlap. C-terminal portion contains Pfam match to entry PF00795 CN_hydrolase, Carbon-nitrogen hydrolase. N-terminal portion contains possible membrane-spanning hydrophobic regions 1 3 19 21 [Reporter]SCO2504 (2E18)_PCR/4448 [Gene]SCO2504/4053 NC_003888 SCC121.07c, glyS, glycyl-tRNA synthetase, len: 460 aa; similar to SW:SYG_MYCTU (EMBL:Z95208) Mycobacterium tuberculosis glycyl-tRNA synthetase (EC 6.1.1.14) (glycine--tRNA ligase) GlyS, 463 aa; fasta scores: opt: 2210 z-score: 2584.3 E(): 0; 68.3% identity in 457 aa overlap and to SW:SYG_BORBU (EMBL:AE001142) Borrelia burgdorferi glycyl-tRNA synthetase (EC 6.1.1.14) (glycine--tRNA ligase) GlyS, 445 aa; fasta scores: opt: 1090 z-score: 1275.8 E(): 0; 47.3% identity in 457 aa overlap. Contains Pfam match to entry PF00587 tRNA-synt_2b and match to Prosite entry PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1 1 3 19 20 [Reporter]SCO7645 (3A18)_PCR/4447 [Gene]SCO7645/4052 NC_003888 SC10F4.18, possible tetR-family transcriptional regulator, len: 237 aa; similar to TR:Q53901 (EMBL:M64683) Streptomyces coelicolor ORF1-4 (actII) ActII, 259 aa; fasta scores: opt: 213 z-score: 261.0 E(): 5.3e-07; 25.3% identity in 233 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 44..65 (+4.27 SD) 1 3 19 19 [Reporter]SCO7210 (4M14)_PCR/4446 [Gene]SCO7210/4051 NC_003888 SC2H12.09, hypothetical protein, len: 153 aa. Highly similar to Streptomyces lincolnensis SW:YMEL_STRLN(EMBL:X95703) hypothetical 17.2 kd protein (150 aa), fasta scores opt: 628 z-score: 750.6 E(): 0 56.4% identity in 149 aa overlap 1 1 9 3 [Reporter]SCP1.180 (19E16)_PCR/178 [Gene]SCP1.180/159 NC_003888 SCP1.180, unknown, len: 103aa; 1 3 19 18 [Reporter]SCO7672 (5I14)_PCR/4445 [Gene]SCO7672/4050 NC_003888 SC4C2.07, possible integral membrane protein, len: 286 aa. Contains possible hydrophobic membrane spanning regions 1 1 9 2 [Reporter]SCO5074 (20M12)_PCR/177 [Gene]SCO5074/158 NC_003888 SCBAC20F6.17, possible dehydratase, len: 214 aa; identical to previously sequenced TR:Q53928 (EMBL:X62373) Streptomyces coelicolor ORF 3, 214 aa. Similar to TR:Q56172 (EMBL:L37334) putative dehydratase from granaticin biosynthesis cluster of Streptomyces violaceoruber Tu22 (221 aa) fasta scores; opt: 349, Z-score: 400.9, E(): 1.1e-14, 35.465% identity (37.195% ungapped) in 172 aa overlap. 1 1 8 22 [Reporter]SCO1230 (1M8)_PCR/175 [Gene]SCO1230/157 NC_003888 2SCG1.05c, possible secreted tripeptidylaminopeptidase, len: 541 aa; almost identical to TR:Q54410 (EMBL:L27466) Streptomyces lividans tripeptidylaminopeptidase precursor, 537 aa and to TR:CAA18977 (EMBL:AL023517) Streptomyces coelicolor putative secreted protease SC1B5.03c, 543 aa; fasta scores: opt: 1804 z-score: 1664.4 E(): 0; 51.0% identity in 547 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold and match to Prosite entry PS00120 Lipases, serine active site. Also contains possible N-terminal region signal peptide sequence 1 1 8 21 [Reporter]SCO0189 (2I8)_PCR/174 [Gene]SCO0189/156 NC_003888 SCJ1.38c, crtU, probable dehydrogenase, a partial CDS, len: >336 aa. Highly similar to Streptomyces griseus TR:P72449 (EMBL:X95596) dehydrogenase (517 aa), fasta scores opt: 1277 z-score: 1459.8 E():0 69.0% identity in 281 aa overlap and Synechocystis sp SW:CRTI_SYNY3 (EMBL; X62574) phytoene dehydrogenase (427 aa), fasta scores opt: 133 z-score: 155.8 E():0.25 24.6% identity in 260 aa overlap.,SCJ12.01c, crtU, probable dehydrogenase, partial CDS, len: >278 aa. Highly similar to Streptomyces griseus TR:P72449 (EMBL:X95596) dehydrogenase (517 aa), fasta scores opt: 1311 z-score: 1471.3 E(): 0 74.0% identity in 269 aa overlap and Synechocystis sp. (strain PCC 6803) SW:CRTI_SYNY3 (EMBL:X62574) phytoene dehydrogenase (EC 1.3.-.-) (phytoene desaturase) (427 aa), fasta scores opt: 219 z-score: 250.2 E(): 1.4e-06 27.6% identity in 254 aa overlap. 1 1 8 20 [Reporter]SCO3123 (3E8)_PCR/173 [Gene]SCO3123/155 NC_003888 SCE66.02, prsA2, ribose-phosphate pyrophosphokinase (EC 2.7.6.1), len: 324 aa; strongly similar to many eg. SW:P14193 (KPRS_BACSU) ribose-phosphate pyrophosphokinase from Bacillus subtilis (317 aa) fasta scores; opt: 1000, z-score: 1178.1, E(): 0, 46.8% identity in 314 aa overlap. Contains Pfam match to entry PF00156 Pribosyltran, Phosphoribosyl transferase domain and Prosite match to PS00103 Purine/pyrimidine phosphoribosyl transferases signature. 1 1 8 19 [Reporter]SCO5524 (4A8)_PCR/172 [Gene]SCO5524/154 NC_003888 SC1C2.05c, possible transmembrane transport protein, len: 476 aa; similar to e.g. B. subtilis TR:P94369 (EMBL:D83026) yxlA (homologous to purine-cytosine permease) (457 aa), fasta scores; opt: 235 z-score: 377.4 E(): 9e-14, 24.0% identity in 475 aa overlap 1 1 8 18 [Reporter]SCO6568 (5M4)_PCR/171 [Gene]SCO6568/153 NC_003888 SC3F9.03, ABC transporter integral membrane protein, len: 339 aa; similar to many involved in sugar transport e.g. RBSC_BACSU ribose transport system permease protein (323 aa), fasta scores; opt: 700 z-score: 958.9 E(): 0, 39.5%identity in 314 aa overlap 1 1 8 17 [Reporter]SCO6671 (6I4)_PCR/170 [Gene]SCO6671/152 NC_003888 SC5A7.21c, unknown, len: 388 aa; Similar to TR:Q9RSB2 (EMBL:AE002054) conserved hypothetical protein from Deinococcus radiodurans (338 aa) fasta scores; opt: 415, Z-score: 392.3, E(): 3.2e-14, 31.333% identity (32.639% ungapped) in 300 aa overlap. Contains nine degenerate PA repeats at C-terminus 1 1 8 16 [Reporter]SCO7290 (7E4)_PCR/169 [Gene]SCO7290/151 NC_003888 SC5H1.02, probable dehydrogenase, len: 325 aa; similar to many e.g. TR:O68447 (EMBL:AF041061), fgd, Mycobacterium smegmatis F420-dependent glucose-6-phosphate dehydrogenase (336 aa), fasta scores; opt: 461 z-score: 553.9 E(): 1.6e-23, 33.4% identity in 335 aa overlap. N-terminus similar to N-terminus of SCD78.18c (EMBL:AL034355) S.coelicolor hypothetical protein (320 aa) (31.7% identity in 183 aa overlap) 1 1 8 15 [Reporter]SCO6686 (8A4)_PCR/168 [Gene]SCO6686/150 NC_003888 SC6G3.02, hypothetical protein, len: 292 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Similar to TR:Q9RJY9 (EMBL:AL133210) Streptomyces coelicolor hypothetical protein SCF37.12, 284 aa; fasta scores: opt: 864 Z-score: 995.3 E(): 8.3e-48; 47.902% identity in 286 aa overlap 2 4 13 21 [Reporter]SCO5139 (2N9)_PCR/6835 [Gene]SCO5139/6229 NC_003888 SCP8.02, dapE, succinyl-diaminopimelate desuccinylase, len: 359 aa; highly similar to SW:DAPE_CORGL (EMBL:X81379) Corynebacterium glutamicum succinyl-diaminopimelate desuccinylase (EC 3.5.1.18) DapE, 369 aa; fasta scores: opt: 1189 z-score: 1383.5 E(): 0; 52.1% identity in 361 aa overlap. Contains Pfam match to entry PF01546 Peptidase_M20, Peptidase family M20/M25/M40 2 4 13 20 [Reporter]SCO0752 (3J9)_PCR/6834 [Gene]SCO0752/6228 NC_003888 SCF81.11c, salO, protease precursor, len: 437 aa; highly similar to but longer than TR:Q54395 (EMBL:D50081) Streptomyces lividans, SalO precursor, 354 aa; fasta scores: opt: 1713 z-score: 1480.6 E(): 0; 89.1% identity in 367 aa overlap, to SW:PRTD_STRGR (EMBL:L29019) Streptomyces griseus serine protease D precursor (EC 3.4.21.-) SgpD, 392 aa; fasta scores: opt: 1097 z-score: 952.8 E(): 0; 53.4% identity in 363 aa overlap and to Streptomyces coelicolor StI11.30c, 360 aa; fasta scores: opt: 1093 z-score: 833.4 E(): 0; 52.8% identity in 362 aa overlap. Contains match to Pfam entry PF00089 trypsin, Trypsin and two matches to Prosite entry PS00134 Serine proteases, trypsin family, histidine active site. Contains also possible N-terminal signal sequence peptide and degenerate repeat x6 GGA(G/D)AG 2 4 13 19 [Reporter]SCO5541 (4F9)_PCR/6833 [Gene]SCO5541/6227 NC_003888 SC1C2.22c, cvnD1, probable ATP /GTP binding protein, len: 174 aa; similar to prokaryotic hypothetical proteins e.g. M. tuberculosis TR:O50391 (EMBL:AL009198) MTV004.19C (193 aa), fasta scores; opt: 718 z-score: 1252.2 E(): 0, 64.9% identity in 168 aa overlap, and more distantly to the yeast GTP-binding protein SEC4_YEAST RAS-related protein SEC4 (215 aa), fasta scores; opt: 111 z-score: 192.5 E(): 0.0018, 25.0% identity in 128 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop). 97.1% identitical to downstream gene SC1C2.18c 2 4 13 18 [Reporter]SCO6399 (5B9)_PCR/6832 [Gene]SCO6399/6226 NC_003888 SC3C8.18c, unknown, len: 171 aa 2 4 13 17 [Reporter]SCO5814 (6N5)_PCR/6831 [Gene]SCO5814/6225 NC_003888 SC5B8.04c, unknown, len: 204 aa 2 4 13 16 [Reporter]SCO5430 (7J5)_PCR/6830 [Gene]SCO5430/6224 NC_003888 SC6A11.06c, possible extracellular solute-binding lipoprotein, len: 458 aa; similar to many proposed solute-binding proteins e.g. TR:P72397 (EMBL:Y07706) putative maltose-binding protein from Streptomyces coelicolor (423 aa) fasta scores; opt: 284, z-score: 316.1, E(): 3.4e-10, 29.1% identity in 443 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein, Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal signal sequence 2 4 13 15 [Reporter]SCO4743 (8F5)_PCR/6829 [Gene]SCO4743/6223 NC_003888 SC6G4.21, unknown, len: 201 aa; similar, in part, to a hypothetical protein from Streptomyces purpurascens TR:Q 54855 (EMBL:X61931) (90 aa), fasta scores; opt: 376 z-score : 584.3 E(): 2.8e-25, 58.5% identity in 94 aa overlap. Cont ains helix-turn-helix motif at aa 28-49 (Score 2176, +6.60 SD) 2 4 13 14 [Reporter]SCO7721 (9B5)_PCR/6828 [Gene]SCO7721/6222 NC_003888 SC8D11.12, conserved hypothetical protein, len: 253 aa; similar to TR:O86750 (EMBL:AL031035) Streptomyces coelicolor hypothetical 28.3 kDa protein SC6A9.18c, 266 aa; fasta scores: opt: 418 z-score: 520.0 E(): 2e-21; 36.6% identity in 246 aa overlap 1 3 19 17 [Reporter]SCO7763 (6E14)_PCR/4444 [Gene]SCO7763/4049 NC_003888 SC5E9.11, possible membrane protein, len: 233aa; contains a possible membrane-spanning hydrophobic region. 2 4 13 13 [Reporter]SCO6252 (10N1)_PCR/6827 [Gene]SCO6252/6221 NC_003888 SCAH10.17c, putative membrane protein, len: 66 aa. Contains possible hydrophobic membrane spanning regions 1 3 19 16 [Reporter]SCO5639 (7A14)_PCR/4443 [Gene]SCO5639/4048 NC_003888 SC6A9.28, unknown, len: 176 aa; this ORF has atypically low GC bias in FramePlot 2 4 13 12 [Reporter]SCO2346 (11J1)_PCR/6826 [Gene]SCO2346/6220 NC_003888 SCC8A.04c, possible hydrolase, len: 221 aa; similar to many e.g. TR:P96428 (EMBL:Z79692) ORF24 from Sinorhizobium meliloti (233 aa) fasta scores; opt: 526, z-score: 576.9, E(): 1.1e-24, TR:AAF18566 (EMBL:AF043232) glycerol-3-phosphate phosphatase from Emericella nidulans (Aspergillus nidulans) (236 aa) fasta scores; opt: 352, z-score: 390.2, E(): 2.9e-14, 31.8% identity in 233 aa overlap. Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase and Prosite match to PS01228 Hypothetical cof family signature 1. 1 3 19 15 [Reporter]SCO6009 (8M10)_PCR/4442 [Gene]SCO6009/4047 NC_003888 SC7B7.06, probable solute-binding protein of transmembrane transport system, len: 403 aa; similar to many eg. XYLF_ECOLI P37387 d-xylose-binding periplasmic protein precursor (330 aa), fasta scores; opt: 619 z-score: 573.4 E(): 8.9e-25, 35.7% identity in 325 aa overlap. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 1 3 19 14 [Reporter]SCO6999 (9I10)_PCR/4441 [Gene]SCO6999/4046 NC_003888 SC8F11.25c, unknown, len: 265 aa. 1 3 19 13 [Reporter]SCO7371 (10E10)_PCR/4440 [Gene]SCO7371/4045 NC_003888 SC9H11.25c, unknown, len: 187 aa. Weakly similar to proteins of unknown function from: Schizosaccharomyces pombe (Fission yeast) TR:CAB60016 (EMBL:AL132779) (203 aa), fasta scores opt: 275 z-score: 313.3 E(): 5.4e-10 25.7% identity in 175 aa overlap and Streptomyces coelicolor TR:CAB53436(EMBL:AL109989) SCJ12.24C (157 aa), fasta scores opt: 139 z-score: 166.4 E(): 0.082 27.0% identity in 122 aa overlap. 1 3 19 12 [Reporter]SCO2620 (11A10)_PCR/4439 [Gene]SCO2620/4044 NC_003888 SCC80.05c, probable cell division trigger factor, len: 468 aa; similar to SW:TIG_BACSU (EMBL:Z75208) Bacillus subtilis trigger factor (vegetative protein 2) Tig, 423 aa; fasta scores: opt: 861 z-score: 823.6 E(): 0; 35.9% identity in 412 aa overlap. Contains and match to Prosite entry 1 3 19 11 [Reporter]SCO3955 (12M6)_PCR/4438 [Gene]SCO3955/4043 NC_003888 SCD78.22c, conserved hypothetical protein, len: 339 aa; similar to TR:Q9HYS2 (EMBL:AE004754) Pseudomonas aeruginosa hypothetical protein PA3323, 291 aa; fasta scores: opt: 1052 Z-score: 1194.1 E(): 7e-59; 57.143% identityin 273 aa overlap. Contains TTA Leu codon: possible target for bldA regulation 1 3 19 10 [Reporter]SCO3269 (13I6)_PCR/4437 [Gene]SCO3269/4042 NC_003888 SCE39.19, possible gntR-family regulator, len: 251 aa; some similarity to many egs. TR:Q07191 (EMBL:AJ005260) KorSA protein from the integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens (259 aa) fasta scores; opt: 327, z-score: 380.9, E(): 6.7e-14, (31.0% identity in 252 aa overlap) and TR:O86763 (EMBL:AL031035) KorSA transcriptional regulator from Streptomyces coelicolor cosmid 6A9 (259 aa) fasta scores; opt: 326, z-score: 379.7, E(): 7.7e-14, (28.9% identity in 249 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. Also contains probable helix-turn-helix (+4.51 SD) 31-52aa. 1 3 19 9 [Reporter]SCO2977 (14E6)_PCR/4436 [Gene]SCO2977/4041 NC_003888 SCE50.05, unknown, len: 399 aa 1 1 8 14 [Reporter]SCO6591 (8M24)_PCR/167 [Gene]SCO6591/149 NC_003888 SC8A6.12c, possible secreted protein, len: 168 aa; c ontains possible N-terminal signal sequence 1 3 19 8 [Reporter]SCO2607 (15A6)_PCR/4435 [Gene]SCO2607/4040 NC_003888 SCC88.18c, sfr, Sfr protein, len: 372 aa; identical to previously sequenced TR:O33623 (EMBL:Y14206) Streptomyces coelicolor Sfr protein and similar to SW:SP5E_BACSU (EMBL:X51419) Bacillus subtilis stage V sporulation protein E, SpoVE, 366 aa; fasta scores: opt: 795 z-score: 897.2 E(): 0; 39.4% identity in 363 aa overlap. Contains Pfam match to entry PF01098 FTSW_RODA_SPOVE, Cell cycle protein and match to Prosite entry PS00428 Cell cycle proteins ftsW / rodA / spoVE signature. Contains also possible hydrophobic membrane spanning regions 1 1 8 13 [Reporter]SCO6466 (9I24)_PCR/166 [Gene]SCO6466/148 NC_003888 SC9C7.02, conserved hypothetical protein, len: 380 aa; similar to many e.g. SW:YXAA_BACSU hypothetical protein from Bacillus subtilis (382 aa) fasta scores; opt: 1045, z-score: 998.9, E(): 0, (46.3% identity in 374 aa overlap) and to SW:GRK_BACSU (EMBL:AB005554) Bacillus subtilis glycerate kinase (EC 2.7.1.31) GlxK, 382 aa; fasta scores: opt: 1045 Z-score: 991.1 E(): 1.4e-47; 46.257% identity in 374 aa overlap 1 1 8 12 [Reporter]SCO2313 (10E24)_PCR/165 [Gene]SCO2313/147 NC_003888 SCC53.04, possible membrane protein, len: 336aa; similar to TR:O69684 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (241 aa) fasta scores; opt: 536, z-score: 436.5, E(): 7.6e-17, 41.2% identity in 233 aa overlap. Contains Pfam match to entry PF01520 Amidase_3, N-acetylmuramoyl-L-alanine amidase and possible hydrophobic membrane spanning region 1 1 8 11 [Reporter]SCO4681 (11A24)_PCR/164 [Gene]SCO4681/146 NC_003888 SCD31.06, probable short chain dehydrogenase, len: 296 aa; similar to TR:CAB56135 (EMBL:AL117669) Streptomyces coelicolor putative short chain dehydrogenase SCF12.09, 249 aa; fasta scores: opt: 1003 z-score: 1070.0 E(): 0; 65.2% identity in 244 aa overlap and to SW:SDR1_PICAB (EMBL:X74115) Picea abies short-chain type dehydrogenase/reductase (EC 1.-.-.-) Sdr1,271 aa; fasta scores: opt: 576 z-score: 619.1 E(): 4.7e-27; 47.8% identity in 251 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus 1 1 8 10 [Reporter]SCO3522 (12M20)_PCR/163 [Gene]SCO3522/145 NC_003888 SCE2.03c, possible transcriptional regulator, len: 111aa; similar to many egs. SW:YW25_MYCTU hypothetical transcriptional regulator from Mycobacterium tuberculosis (118 aa) fasta scores; opt: 348, z-score: 457.9, E(): 3.4e-18, (52.9% identity in 102 aa overlap) and SW:ARSR_STAXY arsenical ersistance operon repressor from Staphylococcus xylosus plasmid pSX267 (104 aa) fasta scores; opt: 126, z-score: 176.8, E(): 0.016, (30.4% identity in 102 aa overlap). Contains Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family. 1 1 8 9 [Reporter]SCO1906 (13I20)_PCR/162 [Gene]SCO1906/144 NC_003888 SCI7.24c, putative secreted protein, len: 490 aa; similar to TR:O69840 (EMBL:AL023517) hypothetical protein from Streptomyces coelicolor (465 aa) fasta scores; opt: 1059, z-score: 1178.4, E(): 0, (48.1% identity in 495 aa overlap). Contains possible N-terminal region signal peptide sequence 1 1 8 8 [Reporter]SCO1611 (14E20)_PCR/161 [Gene]SCO1611/143 NC_003888 SCI35.33c, probable dehydrogenase, len: 260 aa; similar to members of the short-chain dehydrogenases/reductases family e.g. LINX_PSEPA 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (250 aa), fasta scores; opt: 532 z-score: 629.1 E(): 8.5e-28, 36.0% identity in 250 aa overlap. Contains Pfam matches to entry PF00106 adh_short, Alcohol /other, score 230.30, E-value 2.7e-65, and to entry PF00678 adh_short_C2, Short chain dehydrogenase /reductase C-terminus, score 41.20, E-value 2.4e-08 1 1 8 7 [Reporter]SCO3906 (15A20)_PCR/160 [Gene]SCO3906/142 NC_003888 SCH24.28, rpsF, probable 30S ribosomal protein S6, len: 96 aa; similar to many e.g. SW:RS6_BACSU (EMBL:D26185), rpsF, Bacillus subtilis 30S ribosomal protein S6 (95 aa), fasta scores; opt: 249 z-score: 343.8 E(): 7.7e-12, 36.2% identity in 94 aa overlap. Contains Pfam match to entry PF01250 Ribosomal_S6, Ribosomal protein S6, score 148.40, E-value 1.3e-40 and PS01048 Ribosomal protein S6 signature 1 1 8 6 [Reporter]SCO4646 (16M16)_PCR/159 [Gene]SCO4646/141 NC_003888 SCD82.17, secE, preprotein translocase SecE subunit, len: 94 aa; identical to previously sequenced SW:SECE_STRCO (EMBL:D32254) Streptomyces coelicolor preprotein translocase SecE subunit, 94 aa. Contains Pfam match to entry PF00584 SecE, SecE/Sec61-gamma subunits of protein translocation complex and match to Prosite entry PS01067 Protein secE/sec61-gamma signature also possible hydrophobic membrane spanning region 1 1 8 4 [Reporter]SCO4865 (18E16)_PCR/157 [Gene]SCO4865/140 NC_003888 SCK20.06, unknown, len: 306 aa. High G+C content (82.13%) 2 4 13 11 [Reporter]SCO4450 (12F1)_PCR/6825 [Gene]SCO4450/6219 NC_003888 SCD6.28, probable tetR-family transcriptional regulator, len: 215 aa; similar to SW:TCMR_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C transcriptional repressor TcmR, 226 aa; fasta scores: opt: 295 z-score: 356.1 E(): 2.2e-12; 33.5% identity in 218 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 39..60 (+4.31 SD) 2 4 13 10 [Reporter]SCO2851 (13B1)_PCR/6824 [Gene]SCO2851/6218 NC_003888 SCE20.25c, possible membrane protein, len: 237 aa. Similar in parts to many bacterial inner membrane proteins e.g. Streptomyces coelicolor TR:O54569(EMBL:Y16311) inner membrane protein, STH24.05 (431 aa), fasta scores opt: 240 z-score: 285.1 E(): 1.7e-08 25.0% identity in 232 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 2 4 13 9 [Reporter]SCO0127 (13N21)_PCR/6823 [Gene]SCO0127/6217 NC_003888 SCJ21.08, probable multi-domain beta keto-acyl synthase, len: 2240 aa; similar to proteins involved in fatty acid or polyketide biosynthesis e.g. TR:Q44221 Anabaena sp. type 4 polyketide synthases HglD (fragment) (448 aa), fasta scores; opt: 760 z-score: 639.3 E(): 2.9e-28, 38.0% identity in 392 aa overlap; MCAS_MYCBO mycocerosic acid synthase (2110 aa), fasta scores opt: 707 z-score: 586.8 E(): 2.5e-25, 35.4% identity in 560 aa overlap; and TR:O54142 (EMBL:AL021530) S. coelicolor polyketide synthase RedX (982 aa), opt: 658 z-score: 550.3 E(): 2.7e-23, 36.4% identity in 470 aa overlap. Contains Pfam match to entry PF00109 ketoacyl-synt, Beta-ketoacyl synthase, Pfam match to entry PF00698 Acyl_transf, Acyl transferase domain, and 2x Pfam matches to entry PF01377 Thioester_dehyd, Thioester dehydrase 2 4 13 8 [Reporter]SCO0432 (14J21)_PCR/6822 [Gene]SCO0432/6216 NC_003888 SCF51A.10, probable secreted peptidase, len: 1245 aa. Highly similar to Streptomyces albogriseolus TR:P95684 (EMBL; D83672) subtilisin-like protease (1102 aa), fasta scores opt: 1572 z-score: 1550.1 E(): 0 34.4% identity in 1259 aa overlap. Also similar to the much smaller subtilisins e.g. Bacillus sp. (strain TA39) SW:SUBT_BACS9 (EMBL; X62369) subtilisin precursor (EC 3.4.21.62) (420 aa), fasta scores opt: 485 z-score: 483.5 E(): 1.4e-19 38.2% identity in 304 aa overlap. Contains a Pfam match to entry PF00082 Peptidase_S8, Subtilase family and a possible N-terminal signal sequence. 2 4 13 7 [Reporter]SCO1064 (15F21)_PCR/6821 [Gene]SCO1064/6215 NC_003888 SCG22.10c, probable sugar transport integral membrane protein, len: 311 aa; similar to TR:AAF37728 (EMBL:AF086819) Thermomonospora fusca cellobiose transport permease BglA, 302 aa; fasta scores: opt: 664 z-score: 802.8 E(): 0; 38.6% identity in 280 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 2 4 13 6 [Reporter]SCO3887 (16B21)_PCR/6820 [Gene]SCO3887/6214 NC_003888 StH24.09, possible partitioning or sporulation protein, len: 328 aa; previously sequenced as TR:O07327 (EMBL:Y16311), but with different putative start codon. Similar to many e.g. SW:SP0J_BACSU (EMBL:D26185), spo0J, Bacillus subtilis stage 0 sporulation protein (282 aa), fasta scores; opt: 686 z-score: 757.0 E(): 0, 42.7% identity in 262 aa overlap, TR:O05190 (EMBL:U87804), parB, Caulobacter crescentus chromosome partitioning protein (293 aa) (37.1% identity in 264 aa overlap) and TR:O53595 (EMBL:AL021426), parA, Mycobacterium tuberculosis hypothetical protein (344 aa) (51.4% identity in 360 aa overlap). Contains probable helix-turn-helix motif at aa 160-181 (Score 1587, +4.59 SD) 2 4 13 5 [Reporter]SCO2879 (17N17)_PCR/6819 [Gene]SCO2879/6213 NC_003888 SCE6.16, cvnA12, possible membrane protein, len: 551 aa. Similar to many Streptomyces coelicolor proteins e.g. TR:CAB59483 (EMBL:AL132648) putative integral membrane protein, SCI41.13C (541 aa), fasta scores opt: 449 z-score: 340.7 E(): 1.5e-11 27.5% identity in 564 aa overlap. Contains a possible hydrophobic membrane spanning region and coiled-coils between residues 120..140 2 4 13 4 [Reporter]SCO1314 (18J17)_PCR/6818 [Gene]SCO1314/6212 NC_003888 SCBAC36F5.25c, possible sugar acetyltransferase, len: 267 aa; middle region similar to SW:MAA_ECOLI (EMBL:) Escherichia coli maltose O-acetyltransferase (EC 2.3.1.79) Maa or B0459, 182 aa; fasta scores: opt: 264 Z-score: 295.9 bits: 61.9 E(): 7.2e-09; 36.735% identity in 147 aa overlap. Contains 5x Pfam matches to entry PF00132 hexapep, Bacterial transferase hexapeptide (four repeats) 1 3 19 7 [Reporter]SCO3339 (16M2)_PCR/4434 [Gene]SCO3339/4039 NC_003888 SCE7.06c, probable ABC transporter ATP-binding subunit, len: 276aa; similar to many eg. TR:O33189 (EMBL:Z98268) ABC-type transport protein from Mycobacterium tuberculosis (255 aa) fasta scores; opt: 811, z-score: 850.0, E(): 0, (53.8% identity in 251 aa overlap) and TR:Q53626 (EMBL:U43537) ABC protein in mithramycin resistance (320 aa) fasta scores; opt: 491, z-score: 517.8, E(): 1.6e-21, (37.0% identity in 230 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite matches to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 2 4 13 3 [Reporter]SCO1305 (19F17)_PCR/6817 [Gene]SCO1305/6211 NC_003888 SCBAC36F5.16c, conserved hypothetical protein, len: 464 aa; low similarity to SW:Y929_THEMA (EMBL:U24145) Thermotoga maritima hypothetical protein TM0929, 397 aa; fasta scores: opt: 253 Z-score: 253.9 bits: 56.1 E(): 1.6e-06; 25.789% identity in 380 aa overlap. Contains match to Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature and a 6x degenerate repeat: KP(P/M) 1 3 19 6 [Reporter]SCO0042 (17I2)_PCR/4433 [Gene]SCO0042/4038 NC_003888 SCJ4.23, unknown, len: 311 aa 2 4 13 2 [Reporter]SCO7504 (20N13)_PCR/6816 [Gene]SCO7504/6210 NC_003888 SCBAC17A6.37, possible integral membrane binding-protein-dependent transport protein, len: 316aa; similar to many eg. TR:Q9L0B3 (EMBL:AL163003) putative sugar transporter integral membrane protein from Streptomyces coelicolor (313 aa) fasta scores: opt: 949, Z-score: 1058.2, 48.867% identity (50.671% ungapped) in 309 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component; Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and multiple hydrophobic possible membrane spanning regions. 1 3 19 5 [Reporter]SCO1618 (18E2)_PCR/4432 [Gene]SCO1618/4037 NC_003888 SCI41.01c, hypothetical protein, len: >212 aa. Low similarity with C-terminus of TR:BAA80034 (EMBL:AP000060) Aeropyrum pernix 493 aa hypothetical protein APE1049 (493 aa); fasta scores: opt: 183 z-score: 217.5 E(): 9.6e-05; 29.2% identity in 202 aa overlap. Overlaps and extends StI35.40c,SCI35.40c, unknown. partial CDS, len: >68 aa 1 3 19 4 [Reporter]SCO1664 (19A2)_PCR/4431 [Gene]SCO1664/4036 NC_003888 SCI52.06, conserved hypothetical protein, len: 265 aa; similar to TR:O06242 (EMBL:Z95388) Mycobacterium tuberculosis hypothetical 28.3 kDa protein MTCY270.35, 262 aa; fasta scores: opt: 495 Z-score: 595.6 bits: 117.9 E(): 1.4e-25; 50.951% identity in 263 aa overlap 1 3 19 3 [Reporter]SCP1.202 (19M22)_PCR/4430 [Gene]SCP1.202/4035 NC_003888 SCP1.202, unknown, len: 167aa; 1 3 19 2 [Reporter]SCO0978 (20E22)_PCR/4429 [Gene]SCO0978/4034 NC_003888 SCBAC19F3.05c, L-aspartate-alpha-decarboxylase, len: 139 aa: similar to many e.g. SW:P31664 (PAND_ECOLI) L-aspartate-alpha-decarboxylase PanD from Escherichia coli (126 aa) fasta scores; opt: 391, Z-score: 469.1, 50.877% identity (50.877% ungapped) in 114 aa overlap and SW:O06281 (PAND_MYCTU) aspartate 1-decarboxylase PanD from Mycobacterium tuberculosis (139 aa) fasta scores; opt: 572, Z-score: 677.0, 74.167% identity (74.167% ungapped) in 120 aa overlap. Contains Pfam match to entry PF02261 Asp_decarbox, Aspartate decarboxylase. 1 3 18 22 [Reporter]SCO1115 (1E18)_PCR/4427 [Gene]SCO1115/4033 NC_003888 2SCG38.08, hypothetical protein, len: 332 aa; similar to TR:O50524 (EMBL:AL009204) Streptomyces coelicolor hypothetical 34.4 kDa protein SC9B10.17, 330 aa; fasta scores: opt: 263 z-score: 269.2 E(): 1.5e-07; 29.2% identity in 329 aa overlap and to TR:Q9EWI7 (EMBL:AL450223) Streptomyces coelicolor hypothetical protein SC7H9.09c, 332 aa; fasta scores: opt: 619 Z-score: 612.5 E(): 1.7e-26; 45.289% identity in 329 aa overlap 1 3 18 21 [Reporter]SCO5775 (2A18)_PCR/4426 [Gene]SCO5775/4032 NC_003888 SC4H8.14c, gluC, probable glutamate permease, len: 222; similar to many members of the HisMQ subfamily of the binding-protein dependent transporters eg. GLUC_CORGL P48244 glutamate transport system permease protein from Corynebacterium glutamicum (225 aa), fasta scores; opt: 730 z-score: 1209.4 E(): 0, 58.8% identity in 199 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature 1 3 18 20 [Reporter]SCO6808 (3M14)_PCR/4425 [Gene]SCO6808/4031 NC_003888 SC1A2.17c, possible ArsR-family transcriptional regulator, len: 120 aa. Similar to several regulators including: Streptomyces coelicolor TR:Q9X8X8 (EMBL:AL078610) putative transcriptional regulator, SCH35.28C (122 aa), fasta scores opt: 297 z-score: 392.7 E(): 1.8e-14 49.4% identity in 87 aa overlap and Mycobacterium tuberculosis TR:P71941 (EMBL:Z80225) putative transcriptional regulator (126 aa), fasta scores opt: 262 z-score: 348.0 E(): 5.6e-12 38.1% identity in 113 aa overlap. Contains a Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family with the putative helix-turn-helix motif situated between residues 56..77 (+4.54 SD). 1 1 8 3 [Reporter]SCO2237 (19A16)_PCR/156 [Gene]SCO2237/139 NC_003888 SCBAC17D6.04, possible lyase, len: 148 aa; similar to TR:Q9FBS1 (EMBL:AL391072) Streptomyces coelicolor putative lyase SC9A4.33c, 143 aa; fasta scores: opt: 287 Z-score: 354.0 bits: 71.5 E(): 3.9e-12; 41.497% identity in 147 aa overlap. Contains 2x Pfam matches to entry PF00903 Glyoxalase, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily 1 3 18 19 [Reporter]SCO7206 (4I14)_PCR/4424 [Gene]SCO7206/4030 NC_003888 SC2H12.05, possible membrane protein, len: 366 aa. Contains possible membrane spanning hydrophobic domains. 1 1 8 2 [Reporter]SCO0976 (20I12)_PCR/155 [Gene]SCO0976/138 NC_003888 SCBAC19F3.03c, unknown, len: 377 aa: no significant database matches. Alanine-rich C-terminal region. 1 1 7 22 [Reporter]SCO1226 (1I8)_PCR/153 [Gene]SCO1226/137 NC_003888 2SCG1.01c, possible secreted metallopeptidase (fragment), len: >385 aa; similar to TR:CAB76001 (EMBL:AL157916) Streptomyces coelicolor putative neutral zinc metallopeptidase SC3D11.04c, 684 aa; fasta scores: opt: 504 z-score: 545.2 E(): 6.7e-23; 35.2% identity in 406 aa overlap. Contains match to Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Also contains possible N-terminal region signal peptide sequence,2SCG58.26c, possible bifunctional protein (fragment), len: >713 aa; middle region highly similar to SW:APX_STRGR Streptomyces griseus aminopeptidase (EC 3.4.11.-) SgaP, 284 aa; fasta scores: opt: 967 z-score: 978.8 E(): 0; 51.1% identity in 284 aa overlap and C-terminal region similar to C-terminal region of SW:CHI1_BACCI (EMBL:M57601) Bacillus circulans chitinase A1 precursor (EC 3.2.1.14) ChiA1, 699 aa; fasta scores: opt: 283 z-score: 287.0 E(): 1.6e-08; 25.0% identity in 476 aa overlap 1 1 7 21 [Reporter]SCO4423 (2E8)_PCR/152 [Gene]SCO4423/136 NC_003888 SC6F11.21, afsK, serine/threonine protein kinase (fragment), len: >161 aa; identical to N-terminal region of previously sequenced SW:AFSK_STRCO (EMBL:D15062) Streptomyces coelicolor serine/threonine protein kinase AfsK, 799 aa. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and match to Prosite entry PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature,SCD6.01, afsK, serine/threonine protein kinase, len: >671 aa; identical to C-terminal region of SW:AFSK_STRCO (EMBL:D15062) Streptomyces coelicolor serine/threonine protein kinase AfsK, 799 aa and highly similar to the C-terminal region of TR:CAB45488 Streptomyces coelicolor putative serine/threonine protein kinase SC66T3.32c, 783 aa; fasta scores: opt: 521 z-score: 329.5 E(): 7.7e-13; 40.6% identity in 266 aa overlap. Contains Pfam matches to entries PF00069 pkinase, Eukaryotic protein kinase domain and 8x PF01011 Bacterial_PQQ, PQQ enzyme repeat and match to Prosite entry PS00108 Serine/Threonine protein kinases active-site signature 1 1 7 18 [Reporter]SCO5894 (5I4)_PCR/149 [Gene]SCO5894/135 NC_003888 SC3F7.14, redJ, probable thioesterase, len: 280 aa; similar to many e.g. TR:Q00442 (EMBL:X60379) erythomycin A biosynthesis thioesterase (EC 3.1.-.-.) from Saccharopolyspora erythraea (Streptomyces erythraeus) (247 aa), fasta scores; opt: 451 z-score: 472.9 E(): 3.8e-19, 38.0% identity in 237 aa overlap 1 1 7 17 [Reporter]SCO6646 (6E4)_PCR/148 [Gene]SCO6646/134 NC_003888 SC4G2.20, probable transport system permease protein, len: 436 aa; similar to e.g. M. tuberculosis TR:O53861 (EMBL:AL022004) putative membrane transport protein MTV043.42 (419 aa), fasta scores; opt: 771 z-score: 629.6 E(): 8e-28, 37.6% identity in 396 aa overlap 1 1 7 16 [Reporter]SCO0318 (7A4)_PCR/147 [Gene]SCO0318/133 NC_003888 SC5G9.27c, possible oxidoreductase, len: 388 aa; shows weak similarity to many eukaryotic and bacterial oxidoreductases e.g. TR:Q39617 (EMBL:U36752), LpcR-1, Chlamydomonas reinhardtii NADPH:protochlorophyllide oxidoreductase (397 aa), fasta scores; opt: 278 z-score: 296.3 E(): 3.8e-09, 27.4% identity in 343 aa overlap. Similar to TR:O53726 (EMBL:AL021932) Mycobacterium tuberculosis putative oxidoreductase (311 aa) (41.1% identity in 309 aa overlap). Similar to others from S. coelicolor e.g. SCJ9A.19c (cosmid SCJ9A), possible oxidoreductase (311 aa) (39.6% identity in 303 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase 1 1 7 15 [Reporter]SCO2136 (7M24)_PCR/146 [Gene]SCO2136/132 NC_003888 SC6G10.09c, possible secreted protein, len: 347 aa; similar to TR:E1370194 (EMBL:Y13601) hypothetical protein from Streptomyces lividans (250 aa) fasta scores; opt: 371, z-score: 366.3, E(): 4.3e-13, (31.4% identity in 229 aa overlap). Also similar to several examples of Listeria P60 a major extracellular protein involved in the invasion of non-professional phagocytic cells e.g. SW:P60_LISMO from Listeria monocytogenes (484 aa) fasta scores; opt: 351, z-score: 343.1, E(): 8.3e-12, (33.3% identity in 201 aa overlap). Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family at the C-terminus, a probable N-terminal signal sequence and two possible coiled coils. NOTE: highly similar to downstream gene, 6G10.08c, indicating a duplication event; fasta scores: opt: 1042, z-score: 1027.7, E(): 0, (49.9% identity in 339 aa overlap) 1 1 7 14 [Reporter]SCO6584 (8I24)_PCR/145 [Gene]SCO6584/131 NC_003888 SC8A6.05c, probable TPP-requiring enzyme, len: 560 a a; similar to many e.g. ILVB_MYCTU probable acetolactate synthase (EC 4.1.3.18) (547 aa), fasta scores; opt: 733 z-sco re: 1305.6 E(): 0, 36.4% identity in 557 aa overlap. Contai ns PS00187 Thiamine pyrophosphate enzymes signature and Pfam match to entry PF00205 TPP_enzymes, Thiamine pyrophosphate enzymes, score 343.90, E-value 5e-106 1 1 7 13 [Reporter]SCO6076 (9E24)_PCR/144 [Gene]SCO6076/130 NC_003888 SC9B1.23, probable dipeptidase, len: 376aa; similar to many eg. SW:PEPQ_LACDL proline dipeptidase from Lactobacillus delbrueckii subsp. lactis DSM7290 (368 aa) fasta scores; opt: 681, z-score: 740.0, E(): 0, (34.6% identity in 353 aa overlap). 2 4 12 22 [Reporter]SCO1249 (1N9)_PCR/6814 [Gene]SCO1249/6209 NC_003888 2SCG1.24c, unknown, len: 75 aa 2 4 12 21 [Reporter]SCO4164 (2J9)_PCR/6813 [Gene]SCO4164/6208 NC_003888 SCD66.01, probable thiosulfate sulfurtransferase (fragment), len: >168 aa; highly similar to SW:THTR_SACER (EMBL:M29612) Saccharopolyspora erythraea putative thiosulfate sulfurtransferase (EC 2.8.1.1) CysA, 281 aa; fasta scores: opt: 817 z-score: 966.7 E(): 0; 74.8% identity in 163 aa overlap and similar to SW:THTR_ECOLI (EMBL:D10496) Escherichia coli putative thiosulfate sulfurtransferase (EC 2.8.1.1) SseA, 280 aa; fasta scores: opt: 225 z-score: 273.5 E(): 9.2e-08; 28.2% identity in 163 aa overlap. Contains Pfam match to entry PF00581 Rhodanese, Rhodanese-like domain and match to Prosite entry PS00683 Rhodanese C-terminal signature,SCD84.31, cysA, thiosulfate sulfurtransferase (fragment), len: >145 aa; highly similar to SW:THTR_SACE (EMBL:M29612) Saccharopolyspora erythraea putative thiosulfate sulfurtransferase (EC 2.8.1.1) (rhodanase-like protein) CysA, 281 aa; fasta scores: opt: 662 z-score: 820.8 E(): 0; 65.5% identity in 145 aa overlap. Contains Pfam match to entry PF00581 Rhodanese, Rhodanese-like domain 2 4 12 20 [Reporter]SCO1254 (3F9)_PCR/6812 [Gene]SCO1254/6207 NC_003888 2SCG18.01, purB, probable adenylosuccino lyase (fragment), len: >362 aa; highly similar to TR:Q9RHX3 (EMBL:AB00316) Corynebacterium ammoniagenes adenylosuccino lyase PurB, 479 aa; fasta scores: opt: 1625 z-score: 1824.6 E(): 0; 69.4% identity in 363 aa overlap. Contains Pfam match to entry PF00206 lyase_1, Lyase and match to Prosite entry PS00163 Fumarate lyases signature,2SCG1.29, purB, adenylosuccino lyase (fragment), len: >149 aa; highly similar to TR:Q9RHX3 (EMBL:AB003161) Corynebacterium ammoniagenes adenylosuccino lyase PurB, 479 aa; fasta scores: opt: 661 z-score: 805.9 E(): 0; 70.1% identity in 144 aa overlap 2 4 12 19 [Reporter]SCO5535 (4B9)_PCR/6811 [Gene]SCO5535/6206 NC_003888 SC1C2.16, probable carboxyl transferase, len: 527 aa; similar to many e.g. Veillonella parvula TR:Q57079 (EMBL:Z24754) alpha-subunit, methylmalonyl-coa decarboxylase (509 aa), fasta scores; opt: 1929 z-score: 2387.4 E(): 0, 55.9% identity in 510 aa overlap and Myxococcus xanthus TR:O24789 (EMBL:AB007000) propionyl-coa carboxylase (524 aa), fasta scores; opt: 1922 z-score: 2359.6 E(): 0, 55.7% identity in 528 aa overlap 2 4 12 18 [Reporter]SCO6577 (5N5)_PCR/6810 [Gene]SCO6577/6205 NC_003888 SC3F9.12, unknown, len: 282 aa; similar to hypothetical proteins from several organisms e.g. YD83_METJA MJ1383(256 aa), fasta scores; opt: 347 z-score: 391.9 E(): 1.3e-14, 28.5% identity in 260 aa overlap 2 4 12 17 [Reporter]SCO6680 (6J5)_PCR/6809 [Gene]SCO6680/6204 NC_003888 SC5A7.30c, probable oxidoreductase alpha subunit, len: 359 aa; similar to many e.g. VANA_PSES9 vanillate demethylase (monooxygenase) (329 aa), fasta scores; opt: 636 z-score: 695.7 E(): 1.7e-31, 34.6% identity in 338 aa overlap 2 4 12 16 [Reporter]SCO7281 (7F5)_PCR/6808 [Gene]SCO7281/6203 NC_003888 SC5H1.11, clpP3, probable ATP-dependent Clp protease proteolytic subunit 1, len: 217 aa; similar to many e.g. clpP1 (EMBL:AF071885) Streptomyces coelicolor ATP-dependent Clp protease proteolytic subunit 1 (218 aa), fasta scores; opt: 683 z-score: 806.7 E(): 0, 57.6% identity in 177 aa overlap. Also similar to adjacent CDS, SC5H1.12, probable ATP-dependent Clp protease proteolytic subunit 2 (200 aa) (39.3% identity in 201 aa overlap). Contains Pfam match to entry PF00574 CLP_protease, Clp protease, score 281.20, E-value 1.3e-80 and PS00382 Endopeptidase Clp histidine active site 2 4 12 14 [Reporter]SCO2645 (9N1)_PCR/6806 [Gene]SCO2645/6202 NC_003888 SC8E4A.15c, possible integral membrane protein, len: 318 aa; similar to various hypothetical proteins, e.g. TR:O68125 (EMBL:AF010496) Rhodobacter capsulatus hypothetical 32.2 kD protein, 296 aa; fasta scores: opt: 495 z-score: 560.3 E(): 8.4e-24; 36.0% identity in 292 aa overlap and to TR:AAF10091 (EMBL:AE001910) Deinococcus radiodurans conserved hypothetical protein DR0512, 301 aa; fasta scores: opt: 351 z-score: 399.7 E(): 7.5e-15; 30.0% identity in 303 aa overlap. Contains two Pfam matches to entry PF00892 DUF6, Integral membrane protein and various possible hydrophobic membrane spanning regions 1 3 18 18 [Reporter]SCO2379 (5E14)_PCR/4423 [Gene]SCO2379/4029 NC_003888 SC4A7.07, possible acetyltransferase, len: 155 aa; similar to TR:Q55840 (EMBL:D64004) Synechocystis sp hypothetical 23.4 kD protein, 204 aa; fasta scores: opt: 134 z-score: 171.7 E(): 0.035; 26.6% identity in 124 aa overlap and to SW:RIMI_ECOLI (EMBL:X06117) Escherichia coli ribosomal-protein-alanine acetyltransferase (EC 2.3.1.128) RimI, 148 aa; fasta scores: opt: 115 z-score: 151.3 E(): 0.48; 34.5% identity in 55 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 2 4 12 13 [Reporter]SCO6248 (10J1)_PCR/6805 [Gene]SCO6248/6201 NC_003888 SCAH10.13, possible allantoicase, len: 376 aa; similar to SW:ALC_NEUCR (EMBL:J02927) Neurospora crassa allantoicase (EC 3.5.3.4) Alc, 354 aa; fasta scores: opt: 270 z-score: 320.1 E(): 1.9e-10; 35.0% identity in 334 aa overlap 1 3 18 17 [Reporter]SCO7756 (6A14)_PCR/4422 [Gene]SCO7756/4028 NC_003888 SC5E9.04, unknown, len: 74 aa 2 4 12 12 [Reporter]SCO2707 (11F1)_PCR/6804 [Gene]SCO2707/6200 NC_003888 SCC61A.28, possible transferase (membrane-associated), len: 484 aa; similar to many proposed to be involved in LPS biosynthesis e.g. SW:P71241 (WCAJ_ECOLI) putative colanic biosynthesis UDP-glucose lipid carrier transferase from Escherichia coli (464 aa) fasta scores; opt: 493, z-score: 540.1, E(): 1.3e-22, 30.9% identity in 376 aa overlap. Contains possible membrane-spanning hydrophobic regions 1 3 18 15 [Reporter]SCO6005 (8I10)_PCR/4420 [Gene]SCO6005/4027 NC_003888 SC7B7.02, possible lipoprotein, len: 469; has similarity to signal sequence of Mycobacterium leprae hypothetical protein TR:E334742 (EMBL:Z98604) MLCB2052.27 (445 aa), fasta scores; opt: 75 z-score: 199.2 E(): 0.00062, 23.3% identity in 335 aa overlap. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 1 3 18 14 [Reporter]SCO5506 (9E10)_PCR/4419 [Gene]SCO5506/4026 NC_003888 SC8D9.18, possible regulatory protein, len: 1091 aa; limited similarity to many proteins associated with regulatory functions e.g. C-terminal similarity with C-terminal of TR:O86603 (EMBL:AL031155) putative transcriptional regulator from Streptomyces coelicolor (892 aa) fasta scores; opt: 245, z-score: 254.6, E(): 7.1e-07, (30.6% identity in 451 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and a probable helix-turn-helix at 1036-1057aa (+3.98 SD). Also contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 82.70, E-value 7.8e-21. 1 3 18 13 [Reporter]SCO7367 (10A10)_PCR/4418 [Gene]SCO7367/4025 NC_003888 SC9H11.21c, probable membrane efflux protein, len: 446 aa. Similar to many proteins involved in arsenical resistance e.g. Staphylococcus xylosus SW:ARSB_STAXY(EMBL:M80565) arsenical pump membrane protein ArsB (429 aa), fasta scores opt: 255 z-score: 240.6 E(): 6.1e-06 24.1% identity in 440 aa overlap and Mycobacterium tuberculosis TR:P96860(EMBL:Z92774) probable arsenical pump membrane protein (413 aa), fasta scores opt: 730 z-score: 667.0 E(): 1.1e-29 53.7% identity in 445 aa overlap. Contains possible N-terminal region signal sequence peptide and multiple possible membrane spanning hydrophobic domains. 1 3 18 12 [Reporter]SCO4380 (11M6)_PCR/4417 [Gene]SCO4380/4024 NC_003888 SCD10.12, probable acetyl/propionyl CoA carboxylase, beta subunit, len: 532 aa; similar to TR:O86318 (EMBL:Z79700) Mycobacterium tuberculosis AccD2, 529 aa; fasta scores: opt: 2691 z-score: 3002.4 E(): 0; 75.5% identity in 530 aa overlap and to TR:AAF35258 (EMBL:AF059510) Arabidopsis thaliana 3-methylcrotonyl-CoA carbosylase non-biotinylated subunit (EC 6.4.1.4) MccB, 587 aa; fasta scores: opt: 1186 z-score: 1323.2 E(): 0; 48.0% identity in 546 aa overlap. Contains Pfam match to entry PF01039 Carboxyl_trans, Carboxyl transferase domain 1 3 18 11 [Reporter]SCO3951 (12I6)_PCR/4416 [Gene]SCO3951/4023 NC_003888 SCD78.18c, unknown, len: 320 aa; similarity to TR:Q54379 (EMBL:X79146) LmbY(295 aa), part of a gene cluster involved in lincomycin biosynthesis /resistance in Streptomyces lincolnensis, fasta scores; opt: 814, z-score: 584.1, E(): 2.8e-25, (43.8% identity in 290 aa overlap). Also similar to TR:Q49598 (EMBL:U31567) F420-dependent N5,N10-methylenetetrahydromethanopterin reductase from Methanopyruskandleri fasta scores; opt: 191, z-score: 333.9, E(): 2.5e-11, (29.1% identity in 247 aa overlap) 1 3 18 10 [Reporter]SCO3074 (13E6)_PCR/4415 [Gene]SCO3074/4022 NC_003888 SCE25.15c, possible integral membrane protein, len: 195 aa. Contains possible hydrophobic membrane spanning regions 1 3 18 9 [Reporter]SCO2944 (14A6)_PCR/4414 [Gene]SCO2944/4021 NC_003888 SCE59.03c, possible sugar transporter membrane protein, len: 281 aa; similar to TR:CAB59590 (EMBL:AL132662) Streptomyces coelicolor putative sugar transporter inner membrane protein SCF11.13, 285 aa; fasta scores: opt: 730 z-score: 883.7 E(): 0; 42.1% identity in 278 aa overlap and to TR:AAF11008 (EMBL:AE001988) Deinococcus radiodurans ABC transporter, permease protein, MalFG family DR1436, 283 aa; fasta scores: opt: 645 z-score: 781.7 E(): 0; 38.2% identity in 272 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains possible hydrophobic membrane spanning regions 1 1 7 12 [Reporter]SCO2729 (10A24)_PCR/143 [Gene]SCO2729/129 NC_003888 SCC46.14, possible acetyltransferase, len: 202 aa; similar to SW:ATDA_ECOLI (EMBL:D25276) Escherichia coli spermidine N1-acetyltransferase (EC 2.3.1.57) SpeG, 185 aa; fasta scores: opt: 236 z-score: 303.2 E(): 1.8e-09; 27.6% identity in 170 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 3 18 8 [Reporter]SCO1698 (15M2)_PCR/4413 [Gene]SCO1698/4020 NC_003888 SCI30A.19, unknown, len: 153 aa; similar to many of undefined function e.g. TR:P96807 (EMBL:Z92770) hypothetical protein from Mycobacterium tuberculosis (151 aa) fasta scores; opt: 639, z-score: 785.9, E(): 0, (60.8% identity in 148 aa overlap) and SW:NODN_RHILV nodulation protein from Rhizobium leguminosarum (161 aa) fasta scores; opt: 388, z-score: 482.2, E(): 1.6e-19, (44.8% identity in 145 aa overlap) 1 1 7 10 [Reporter]SCO2934 (12I20)_PCR/141 [Gene]SCO2934/128 NC_003888 SCE19A.34c, possible secreted protein, len: 259 aa; unknown function, similar to parts of hypothetical proteins e.g. TR:O53340 (EMBL:AL021646) Mycobacterium tuberculosis hypothetical protein (340 aa), fasta scores; opt: 396 z-score: 436.5 E(): 5.6e-17, 34.4% identity in 337 aa overlap, but with significant indels with respect to that sequence. Very weak similarity to the C-termini of ATP-dependent proteases e.g. SW:LON_CAUCR (EMBL:U56652), lon, Caulobacter crescentus ATP-dependent protease (799 aa) (31.5% identity in 178 aa overlap). Contains a probable N-terminal signal sequence 1 1 7 9 [Reporter]SCO3144 (13E20)_PCR/140 [Gene]SCO3144/127 NC_003888 SCE66.23, possible two-component system response regulator, len: 224aa; similar to many proposed regulators eg. TR:O53856 (EMBL:AL022004) putative response regulator from Mycobacterium tuberculosis (216 aa) fasta scores; opt: 352, z-score: 397.3, E(): 1.2e-14, 36.9% identity in 217 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain and Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family 1 1 7 8 [Reporter]SCO0803 (14A20)_PCR/139 [Gene]SCO0803/126 NC_003888 SCF43.14c, possible RNA polymerase sigma factor, len: 330 aa. Similar to Mycobacterium smegmatis TR:O05767 (EMBL:U87307) extracytoplasmic function alternative sigma factor SigE (204 aa), fasta scores opt: 234 z-score: 278.1 E(): 4.1e-08 29.1% identity in 175 aa overlap. Also highly similar to many other putative sigma factors e.g. Streptomyces coelicolor TR:Q9X8P3 (EMBL:AL049754) putative RNA polymerase sigma factor SCH10.04C (294 aa), fasta scores opt: 915 z-score: 1056.2 E():0 52.9% identity in 293 aa overlap. 1 1 7 7 [Reporter]SCO3932 (15M16)_PCR/138 [Gene]SCO3932/125 NC_003888 SCQ11.15c, probable gntR-family transcriptional regulator, len: 295 aa; similar to many e.g. TR:Q07191 (EMBL:Z19589), KorSA, Streptomyces ambofaciens plasmid pSAM2 transcriptional repressor (259 aa), fasta scores; opt: 292 z-score: 359.5 E(): 1.1e-12, 30.4% identity in 253 aa overlap. Also similar to CDS from other putative integrated plasmids in S.coelicolor e.g. SCE39.09c (EMBL:AL049573), korSA, regulatory protein (259 aa) (30.8% identity in 253 aa overlap). Part of a putative integrated plasmid similar to pSAM2. Contains probable helix-turn-helix motif at aa 35-56 (Score 1296, +3.60 SD). Contains Pfam match to PF00392 gntR, Bacterial regulatory proteins, gntR family and PS00043 Bacterial regulatory proteins, gntR family signature 1 1 7 6 [Reporter]SCO1776 (16I16)_PCR/137 [Gene]SCO1776/124 NC_003888 SCI51.16c, pyrG, probable CTP synthetase, len: 549 aa; highly similar to many CTP synthetases e.g. SW:PYRG_MYCTU (EMBL:Z98268, U88301), pyrG, Mycobacterium tuberculosis and Mycobacterium bovis CTP synthetase (586 aa), fasta scores; opt: 2622 z-score: 2909.4 E(): 0, 70.3% identity in 552 aa overlap. Contains PS00442 Glutamine amidotransferases class-I active site 1 1 7 5 [Reporter]SCO0593 (17E16)_PCR/136 [Gene]SCO0593/123 NC_003888 SCF55.17c, unknown, len: 275 aa 1 1 7 4 [Reporter]SCO1634 (18A16)_PCR/135 [Gene]SCO1634/122 NC_003888 SCI41.17c, putative small membrane protein, len: 64 aa. Contains probable coiled-coil from 28 to 64 (37 residues, max score: 1.439, probability 0.85) and possible hydrophobic membrane spanning region 1 1 7 3 [Reporter]SCP1.227 (19M12)_PCR/134 [Gene]SCP1.227/121 NC_003888 SCP1.227, unknown, len: 160aa; previously sequenced and annotated as TR:Q9JN73 (EMBL:AJ276673). 1 1 7 2 [Reporter]SCO4987 (20E12)_PCR/133 [Gene]SCO4987/120 NC_003888 2SCK36.10c, possible D-amino acid deaminase, len: 433 aa; similar to TR:Q45085 (EMBL:AF158699) Burkholderia cepacia D-serine deaminase Dsd, 432 aa; fasta scores: opt: 919 Z-score: 1026.2 bits: 199.0 E(): 1.5e-49; 41.259% identity in 429 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 18 7 [Reporter]SCO3721 (16I2)_PCR/4412 [Gene]SCO3721/4019 NC_003888 SCH35.03, probable carbonic anhydrase, len: 207aa; similar to many (both prokayote and eukaryote) egs. TR:Q39589 (EMBL:U41189) an algal mitochondrial carbonic anhydrase from Chlamydomonas reinhardtii (267 aa) fasta scores; opt: 431, z-score: 502.8, E(): 1.1e-20, (37.9% identity in 198 aa overlap) and SW:CYNT_ECOLI carbonic anhydrase from Escherichia coli (219 aa) fasta scores; opt: 227, z-score: 271.0, E(): 9.1e-08, (30.9% identity in 178 aa overlap). Contains Prosite match to PS00704 Prokaryotic-type carbonic anhydrases signature 1 and Pfam match to entry PF00484 Pro_CA, Prokaryotic-type carbonic anhydrases. 1 3 18 6 [Reporter]SCO3830 (17E2)_PCR/4411 [Gene]SCO3830/4018 NC_003888 SCGD3.31c, bkdB2, probable branched-chain alpha keto acid dehydrogenase E1 beta subunit, len: 334 aa; similar to E1 beta subunits from pyruvate dehydrogenase complexes and branched-chain alpha keto acid dehydrogenases e.g. TR:Q53593 (EMBL:U1716), BkdB, Streptomyces avermitilis E1-beta branched-chain alpha keto acid dehydrogenase (334 aa), fasta scores; opt: 2063 z-score: 2427.3 E(): 0, 91.0% identity in 334 aa overlap and SW:ODPB_BACST (EMBL:X53560), PdhB, Bacillus stearothermophilus pyruvate dehydrogenase complex E1 beta subunit (324 aa) (45.5% identity in 319 aa overlap). Highly similar to SCGD3.17c, probable branched-chain alpha keto acid dehydrogenase E1 beta subunit (326 aa) (55.2% identity in 324 aa overlap), in a duplication of this region downstream 1 3 18 5 [Reporter]SCO0503 (18A2)_PCR/4410 [Gene]SCO0503/4017 NC_003888 SCF34.22, hypothetical protein, len: 326 aa; unknown function, shows weak similarity to the N-terminal half of SCH10.22C (EMBL:AL049754) Streptomyces coelicolor putative lipase/esterase (301 aa), fasta scores; opt: 178 z-score: 202.4 E(): 0.00065, 30.1% identity in 163 aa overlap 1 3 18 4 [Reporter]SCP1.216 (18M22)_PCR/4409 [Gene]SCP1.216/4016 NC_003888 SCP1.216, unknown, len: 173aa; part of possible transposon comprising CDSs SCP1.14, SCP1.15 and SCP1.16. 1 3 18 3 [Reporter]SCO4014 (19I22)_PCR/4408 [Gene]SCO4014/4015 NC_003888 2SC10A7.18c, conserved hypothetical protein, len: 119 aa; similar to SW:YTFH_ECOLI (EMBL:U14003) Escherichia coli hypothetical 14.1 kDa protein in RplI-CpdB intergenic region YtfH, 126 aa; fasta scores: opt 376 z-score: 479.2 E(): 3.5e-19; 56.1% identity in 98 aa overlap. Contains Pfam match to entry PF01638 DUF24, Protein of unknown function DUF24 1 3 18 2 [Reporter]SCO3986 (20A22)_PCR/4407 [Gene]SCO3986/4014 NC_003888 SCBAC25E3.23, possible GntR-family transcriptional regulator, len: 243 aa: similar to TR:Q9X8D7 (EMBL:AL049573) putative GntR-family regulator from Streptomyces coelicolor (243 aa) fasta scores; opt: 1025, Z-score: 1169.6, 67.083% identity (67.083% ungapped) in 240 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and Prosite match to PS00043 Bacterial regulatory proteins, gntR family signature. 1 3 17 22 [Reporter]SCO1110 (1A18)_PCR/4405 [Gene]SCO1110/4013 NC_003888 2SCG38.03, probable secreted lyase, len: 266 aa; similar to TR:Q04701 (EMBL:M94691) Fusarium solani pectate lyase A precursor (EC 4.2.2.2) PelA, 242 aa; fasta scores: opt: 605 z-score: 664.1 E(): 1.5e-29; 43.6% identity in 236 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 17 21 [Reporter]SCO5872 (2M14)_PCR/4404 [Gene]SCO5872/4012 NC_003888 SC2E9.13, kdpE, two-component trascriptional regulator, len: 227, similar to E.coli turgor pressure regulator KDPE_ECOLI P21866 kdp operon transcriptional regulatory protein (225 aa), fasta scores; opt: 752 z-score: 1090.9 E(): 0, 51.1% identity in 225 aa overlap. Contains Pfam match to entry trans_reg_C PF00486, Transcriptional regulatory proteins, C terminal, score 96.22 1 3 17 20 [Reporter]SCO6804 (3I14)_PCR/4403 [Gene]SCO6804/4011 NC_003888 SC1A2.13, unknown, len: 127 aa. 1 1 6 22 [Reporter]SCO5253 (1E8)_PCR/131 [Gene]SCO5253/119 NC_003888 2SC7G11.15, unknown, len: 73 aa 1 3 17 19 [Reporter]SCO5485 (4E14)_PCR/4402 [Gene]SCO5485/4010 NC_003888 SC2A11.19, small hydrophobic membrane protein, len: 97 aa. Contains possible hydrophobic membrane spanning regions 1 1 6 21 [Reporter]SCO1393 (2A8)_PCR/130 [Gene]SCO1393/118 NC_003888 SC1A8A.13, acsA, acetoacetyl-CoA synthetase, len: 658 aa; similar to many eg. TR:BAA90828 (EMBL:AB026291) acetoacetyl-CoA synthetase from Rattus norvegicus (Rat) (672 aa) fasta scores; opt: 1133, z-score: 1295.8, E(): 0, 39.9% identity in 667 aa overlap. Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and Prosite match to PS00455 Putative AMP-binding domain signature. 1 1 6 20 [Reporter]SCO5595 (3M4)_PCR/129 [Gene]SCO5595/117 NC_003888 SC2E1.12, rplS, 50S ribosomal protein L19, len: 116aa; highly similar to many e.g. RL19_MYCTU (113 aa), fastascores; opt: 571 z-score: 1138.4 E(): 0, 77.3% identity in110 aa overlap. Contains PS01015 Ribosomal protein L19 signature 1 1 6 19 [Reporter]SCO6514 (4I4)_PCR/128 [Gene]SCO6514/116 NC_003888 SC1E6.23, unknown, len: 92 aa 1 1 6 18 [Reporter]SCO6358 (5E4)_PCR/127 [Gene]SCO6358/115 NC_003888 SC3A7.26, unknown, len: 129 aa; similar to hypothetical proteins from M. tuberculosis and M. leprae e.g. M. tuberculosis TR:O32909 (EMBL:Z83859) MTCY359.27 (142 aa), fasta scores; opt: 201 z-score: 269.6 E(): 9.1e-08, 39.3% identity in 112 aa overlap 1 1 6 17 [Reporter]SCO6642 (6A4)_PCR/126 [Gene]SCO6642/114 NC_003888 SC4G2.16c, questionable ORF, len: 97 aa 1 1 6 16 [Reporter]SCO4854 (6M24)_PCR/125 [Gene]SCO4854/113 NC_003888 SC5G8.22c, possible integral membrane protein, len: 206aa; weakly similar to many eg. TR:P72920 (EMBL:D90901) hypothetical protein from Synechocystis sp. (strain PCC 6803) (181 aa) fasta scores; opt: 183, z-score: 238.4, E(): 8e-06, 28.8% identity in 146 aa overlap. Contains possible membrane-spanning hydrophobic regions. 1 1 6 15 [Reporter]SCO2132 (7I24)_PCR/124 [Gene]SCO2132/112 NC_003888 SC6G10.05c, possible glycosyl transferase, len: 412 aa; similar to TR:O53522 (EMBL:AL021957) hypothetical protein from Mycobacterium tuberculosis (399 aa) fasta scores; opt: 1276, z-score: 1452.2, E(): 0, (51.9% identity in 391 aa overlap) and SW:WCAL_SALTY putative colanic acid biosynthesis glycosyl transferase from the Rfb (O antigen) gene cluster of Salmonella typhimurium (406 aa) fasta scores; opt: 291, z-score: 334.2, E(): 2.6e-11, (29.0% identity in 300 aa overlap). Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1 1 1 6 13 [Reporter]SCO6072 (9A24)_PCR/122 [Gene]SCO6072/111 NC_003888 SC9B1.19, unknown, len: 666aa; similar to many hypothetical proteins from eukaryotes eg. TR:O45417 (EMBL:Z92832) from Caenorhabditis elegans (450 aa) fasta scores; opt: 395, z-score: 410.1, E(): 1.6e-15, (28.0% identity in 447 aa overlap). 1 1 6 12 [Reporter]SCO2746 (10M20)_PCR/121 [Gene]SCO2746/110 NC_003888 SCC57A.17, ABC transporter protein, ATP binding component, len: 517 aa. Highly similar to many including: Escherichia coli SW:RBSA_ECOLI(EMBL:M13169) ribose transport ATP-binding protein RbsA (501 aa), fasta scores opt: 1421 z-score: 1446.1 E(): 0 47.5% identity in 493 aa overlap and Streptomyces coelicolor TR:O69942(EMBL:AL023862) ABC transporter ATP binding protein SC3F9.02 (505 aa), fasta scores opt: 1495 z-score: 1521.0 E(): 0 50.2% identity in 494 aa overlap. Contains Prosite hits to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also contains 2x Pfam matches to entry PF00005 ABC_tran, ABC transporter. 1 3 17 18 [Reporter]SCO2375 (5A14)_PCR/4401 [Gene]SCO2375/4009 NC_003888 SC4A7.03, possible secreted protein, len: 406 aa; similar to various hypothetical proteins, e.g. TR:Q9X8R0 (EMBL:AL049754) Streptomyces coelicolor hypothetical 33.9 kD protein, SCH10.21c, 329 aa; fasta scores: opt: 634 z-score: 714.7 E(): 2e-32; 39.3% identity in 346 aa overlap. Contains possible N-terminal signal peptide sequence 1 3 17 17 [Reporter]SCO6522 (6M10)_PCR/4400 [Gene]SCO6522/4008 NC_003888 SC5C7.07, unknown, len: 480 aa 1 3 17 16 [Reporter]SCO6782 (7I10)_PCR/4399 [Gene]SCO6782/4007 NC_003888 SC6A5.31c, probable zinc-binding alcohol dehydrogenase, len: 363aa; similar to many eg. TR:Q59096 (EMBL:U61983) benzyl alcohol dehydrogenase from Acinetobacter calcoaceticus (371 aa) fasta scores; opt: 948, z-score: 937.8, E(): 0, (42.8% identity in 360 aa overlap). Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and Prosite match to PS00059 Zinc-containing alcohol dehydrogenases signature. 1 3 17 15 [Reporter]SCO5309 (8E10)_PCR/4398 [Gene]SCO5309/4006 NC_003888 SC6G9.24c, conserved hypothetical protein, len: 365 aa; unknown function, similar to hypothetical proteins from Mycobacterium tuberculosis, Bacillus subtilis and Archaeoglobus fulgidus e.g. TR:O05866 (EMBL:Z95209) Mycobacterium tuberculosis hypothetical protein (273 aa), fasta scores; opt: 648 z-score: 651.9 E(): 5.6e-29, 36.5% identity in 274 aa overlap. An alternative start codon is present as codon 4 1 3 17 14 [Reporter]SCO5501 (9A10)_PCR/4397 [Gene]SCO5501/4005 NC_003888 SC8D9.13, probable Glu-tRNA-Gln amidotransferase subunit B, gatB, len: 504aa; similar to many eg. TR:O30509 (EMBL:AF008553) Glu-tRNA-Gln amidotransferase subunit B, gatB, from Bacillus subtilis (476 aa) fasta scores; opt: 886, z-score: 983.5, E(): 0, (42.9% identity in 485 aa overlap). 1 3 17 13 [Reporter]SCO2500 (10M6)_PCR/4396 [Gene]SCO2500/4004 NC_003888 SCC121.03, possible membrane protein, len: 142 aa 1 3 17 12 [Reporter]SCO4376 (11I6)_PCR/4395 [Gene]SCO4376/4003 NC_003888 SCD10.08, unknown, len: 169 aa. Contains 3x degenerate repeat: T(T/A)P and a 2x conserved repeat TTDAA 1 3 17 11 [Reporter]SCO4805 (12E6)_PCR/4394 [Gene]SCO4805/4002 NC_003888 SCD63A.16, hypothetical protein, len: 348 aa; similar to SW:YEHP_ECOLI (EMBL:) Escherichia coli hypothetical 42.1 kD protein in MolR-BglX intergenic region YehP, 378 aa; fasta scores: opt: 721 z-score: 816.7 E(): 0; 35.7% identity in 378 aa overlap 1 3 17 10 [Reporter]SCO3069 (13A6)_PCR/4393 [Gene]SCO3069/4001 NC_003888 SCE25.10c, possible integral membrane protein, len: 114 aa. Contains possible hydrophobic membrane spanning regions 1 1 6 11 [Reporter]SCO4628 (11I20)_PCR/120 [Gene]SCO4628/109 NC_003888 SCD39.28, putative regulator, len: 269 aa; identical to C-terminal region of previously sequenced SW:YIMA_STRCO (EMBL:X15866) Streptomyces coelicolor hypothetical 22.9 kDa protein in Imp region (ORFA), 208 aa and similar to TR:Q9X8D2 (EMBL:AL049573) Streptomyces coelicolor KorSA regulatory protein, 259 aa; fasta scores: opt: 389 z-score: 454.8 E(): 7.3e-18; 31.2% identity in 253 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 1 3 17 9 [Reporter]SCO0269 (14M2)_PCR/4392 [Gene]SCO0269/4000 NC_003888 SCF1.11, unknown, len: 1053aa; similar to large proteins thought to be involved in the post-translational modification of lantibiotics eg. SW:SPAB_BACSU subtilin modification protein from Bacillus subtilis (1030 aa) fasta scores; opt: 346, z-score: 367.5, E(): 4.2e-13, (22.6% identity in 1041 aa overlap). 1 1 6 10 [Reporter]SCO4306 (12E20)_PCR/119 [Gene]SCO4306/108 NC_003888 SCD95A.39c, unknown, len: 189 aa 1 1 6 9 [Reporter]SCO0406 (13A20)_PCR/118 [Gene]SCO0406/107 NC_003888 SCF51.05, unknown, len: 237 aa 1 1 6 8 [Reporter]SCO0084 (14M16)_PCR/117 [Gene]SCO0084/106 NC_003888 SCJ11.13, hypothetical protein, len: 152 aa; similar to TR:Q9S1Q3 (EMBL:AL109972) Streptomyces coelicolor hypothetical protein SCJ9A.17, 175 aa; fasta scores: opt: 683 Z-score: 803.5 E(): 4e-37; 68.421% identity in 152 aa overlap 1 1 6 7 [Reporter]SCO4972 (15I16)_PCR/116 [Gene]SCO4972/105 NC_003888 2SCK31.32, possible dehydrogenase, len: 806 aa; similar to the C-terminal region of SW:XDH_MOUSE (EMBL:X75129) Mus musculus xanthine dehydrogenase/oxidase Xdh, 1335 aa; fasta scores: opt: 1456 z-score: 1610.8 E(): 0; 43.3% identity in 767 aa overlap. Contains Pfam match to entry PF01315 Ald_Xan_dh_C, Aldehyde oxidase and xanthine dehydrogenase, C terminus 1 1 6 6 [Reporter]SCO3464 (16E16)_PCR/115 [Gene]SCO3464/104 NC_003888 SCE46.21, hypothetical protein, len: 210 aa; identical to N-terminal region of previously sequenced TR:BAA84086 (EMBL:AB032065) Streptomyces coelicolor hypothetical 47.9 kD protein, 446 aa and similar to TR:P74231 (EMBL:D90913) Synechocystis sp. (strain PCC 6803) hypothetical 23.5 kD protein, 211 aa; fasta scores: opt: 327 z-score: 394.6 E(): 1.2e-14; 36.5% identity in 189 aa overlap. Contains plasmid SCP1 integration target site 1 1 6 5 [Reporter]SCO3145 (17A16)_PCR/114 [Gene]SCO3145/103 NC_003888 SCE66.24c, possible secreted protein, len: 497 aa; similar to TR:Q9K3R8 (EMBL:AL359989) Streptomyces coelicolor putative membrane protein SCE66.25c, 490 aa; fasta scores: opt: 430 Z-score: 424.1 E(): 5.4e-16; 33.012% identity in 518 aa overlap. Contains possible N-terminal signal sequence 1 1 6 4 [Reporter]SCO0214 (18M12)_PCR/113 [Gene]SCO0214/102 NC_003888 SCJ12.26, hypothetical protein, len: 137 aa. Some similarity to several hypotheticals e.g. Mycobacterium tuberculosis TR:O53625 (EMBL; AL021428) hypothetical 16.6 KD protein (152 aa), fasta scores opt: 291 z-score: 372.0 E(): 2.3e-13 46.5% identity in 129 aa overlap. Also similar to TR:Q9RI49 (EMBL:AL109989) S. coelicolor SCJ12.09c (146 aa), fasta scores opt: 524 z-score: 550.7 E(): 2e-25 62.2% identity in 135 aa overlap 1 1 6 3 [Reporter]SCO1668 (19I12)_PCR/112 [Gene]SCO1668/101 NC_003888 SCI52.10c, unknown, len: 262 aa 1 1 6 2 [Reporter]SCO5351 (20A12)_PCR/111 [Gene]SCO5351/100 NC_003888 SCBAC5H2.20, possible regulatory protein, len: 167 aa; similar to the N-terminal domain of several regulatory proteins, e.g. TR:Q9X6C1 (EMBL:AF135389) Tolypothrix PCC7601 DNA binding response regulator RpaA, 247 aa; fasta scores: opt: 332 z-score: 343.7 E(): 1.4e-11; 42.0% identity in 119 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain 1 4 15 12 [Reporter]SCO2275 (11A5)_PCR/6366 [Gene]SCO2275/5799 NC_003888 SCC75A.21, possible lipoprotein, len: 384 aa. Similar to several proteins of undefined function including: Escherichia coli TR:BAA35799(EMBL:D90739) (362 aa), fasta scores opt: 783 z-score: 851.0 E():0 39.2% identity in 365 aa overlap and Bacillus subtilis SW:YWBM_BACSU(EMBL:X73124) (385 aa), fasta scores opt: 675 z-score: 734.3 E():0 34.9% identity in 387 aa overlap. Contains a N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 1 4 15 10 [Reporter]SCO3018 (13I1)_PCR/6364 [Gene]SCO3018/5798 NC_003888 SCE33.20, possible regulatory protein, len: 329 aa; similar to TR:O69660 (EMBL:AL022121) Mycobacterium tuberculosis putative regulatory protein MTV025.040, 358 aa; fasta scores: opt: 1523 z-score: 1708.1 E(): 0; 70.9% identity in 330 aa overlap and to TR:CAA22801 (EMBL:AL035212) Streptomyces coelicolor putative regulatory protein, SC9B2.21c, 332 aa; fasta scores: opt: 956 z-score: 937.8 E(): 0; 50.7% identity in 306 aa overlap. Contains Pfam match to entry PF01078 Mg_chelatase, Magnesium chelatase, subunit ChlI 1 4 15 9 [Reporter]SCO5022 (14E1)_PCR/6363 [Gene]SCO5022/5797 NC_003888 SCK15.24, possible lipoprotein, len: 220 aa; similar to Streptomyces coelicolor SCK15.23, 221 aa; fasta scores: opt: 736 z-score: 703.5 E(): 1.3e-33; 54.3% identity in 221 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 1 4 15 8 [Reporter]SCO1716 (15A1)_PCR/6362 [Gene]SCO1716/5796 NC_003888 SCI11.05, possible gntR-family transcriptional regulator, len: 249 aa; weakly similar to many trancriptional regulators e.g. SW:FARR_ECOLI (EMBL:X15790), FarR, Escherichia coli fatty acyl responsive regulator (240 aa), fasta scores; opt: 213 z-score: 265.9 E(): 1.8e-07, 25.0% identity in 232 aa overlap. Similar to others from S.coelicolor e.g. SCE39.14c (EMBL:AL049573) S.coelicolor possible gntR-family regulator (243 aa) (29.5% identity in 224 aa overlap). Contains probable helix-turn-helix motif at aa 42-63 (Score 1296, +3.60 SD). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 1 4 15 7 [Reporter]SCO0815 (15M21)_PCR/6361 [Gene]SCO0815/5795 NC_003888 SCF43A.05, conserved hypothetical protein, len: 250 aa; unknown function, similar to other hypothetical proteins e.g. TR:O07020 (EMBL:Z94043) Bacillus subtilis hypothetical protein (238 aa), fasta scores; opt: 720 z-score: 887.0 E(): 0, 46.1% identity in 245 aa overlap. Also similar to the C-terminal half of many Fe-S proteins e.g. SW:GLPC_ECOLI (EMBL:M20938), glpC, Escherichia coli anaerobic glycerol-3-phosphate dehydrogenase subunit C (396 aa) (26.7% identity in 255 aa overlap) 1 4 15 6 [Reporter]SCO3220 (16I21)_PCR/6360 [Gene]SCO3220/5794 NC_003888 SCE8.13c, putative secreted protein, len: 142 aa; contains alanine-rich N-terminal region and possible N-terminal region signal peptide sequence 1 4 15 5 [Reporter]SCO3642 (17E21)_PCR/6359 [Gene]SCO3642/5793 NC_003888 SCH10.20c, hypothetical membrane protein, len: 420 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains several hydrophobic, probable membrane-spanning regions. Contains PS00133 Zinc carboxypeptidases, zinc-binding region 2 signature 1 4 15 4 [Reporter]SCO3666 (18A21)_PCR/6358 [Gene]SCO3666/5792 NC_003888 SCH44.06c, possible regulatory protein, len: 394 aa; similar to TR:Q56084 (EMBL:D63904) Streptomyces thermoviolaceus ESA36 (erythropoiesis-stimulating activity), 344 aa; fasta scores: opt: 380 z-score: 432.8 E(): 1.2e-16; 31.1% identity in 286 aa overlapand to Streptomyces colicolor SCH44.04, 336 aa; fasta scores: opt: 952 z-score: 855.5 E(): 0; 48.1% identity in 320 aa overlap and to SCH44.05, 327 aa; fasta scores: opt: 849 z-score: 764.1 E(): 0; 45.2% identity in 323 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and possible helix-turn-helix motif at residues 328..349 (+3.19 SD) 1 4 15 3 [Reporter]SCP1.155 (19M17)_PCR/6357 [Gene]SCP1.155/5791 NC_003888 SCP1.155, unknown, len: 318aa; 1 4 15 2 [Reporter]SCO7505 (20E17)_PCR/6356 [Gene]SCO7505/5790 NC_003888 SCBAC17A6.38, possible integral membrane binding-protein-dependent transport protein, len: 299aa; similar to many eg. TR:Q9L0B4 (EMBL:AL163003) putative sugar transporter integral membrane protein from Streptomyces coelicolor (313 aa) fasta scores: opt: 905, Z-score: 1049.4, 50.000% identity (51.799% ungapped) in 288 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component; Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and multiple hydrophobic possible membrane spanning regions. 1 4 14 22 [Reporter]SCO1282 (1E13)_PCR/6354 [Gene]SCO1282/5789 NC_003888 2SCG18.29c, probable oxidoreductase, len: 192 aa; similar to TR:Q9ZA25 (EMBL:AJ011500) Streptomyces violaceoruber putative FMN:NADH oxidoreductase Gra-ORF34, 179 aa; fasta scores: opt: 320 z-score: 377.4 E(): 1.5e-13; 41.3% identity in 167 aa overlap. Contains Pfam match to entry PF01613 Flavin_Reduct, Flavin reductase like domain 1 4 14 21 [Reporter]SCO2614 (2A13)_PCR/6353 [Gene]SCO2614/5788 NC_003888 SCC88.25c, fpgS, folylpolyglutamate synthase, len: 444 aa; identical to previously sequenced TR:O08416 (EMBL:Y13070) Streptomyces coelicolor folylpolyglutamate synthase (EC 6.3.2.17), 444 aa and similar to SW:FOLC_BACSU (EMBL:L04520) Bacillus subtilis folylpolyglutamate synthase (EC 6.3.2.17), FolC, 430 aa; fasta scoreS: opt: 622 z-score: 688.7 E(): 6.1e-31; 32.0% identity in 438 aa overlap. Contains Pfam match to entry PF01225 Mur_ligase, Mur ligase family and matches to Prosite entries PS00398 Site-specific recombinases signature 2 and PS01012 Folylpolyglutamate synthase signature 2 1 4 14 20 [Reporter]SCO5898 (3M9)_PCR/6352 [Gene]SCO5898/5787 NC_003888 SC10A5.03, redF, probable membrane protein, len: 226 aa. Contains possibe hydrophobic membrane spanning regions 1 4 14 19 [Reporter]SCO6936 (4I9)_PCR/6351 [Gene]SCO6936/5786 NC_003888 SC1G8.08, unknown, len: 217 aa. Weakly similar to Streptomyces coelicolor TR:CAB56672 (EMBL:AL121596) hypothetical protein, SCF51A.20(252 aa), fasta scores opt: 111 z-score: 140.7 E(): 2.2 27.9% identity in 190 aa overlap. 1 4 14 18 [Reporter]SCO6403 (5E9)_PCR/6350 [Gene]SCO6403/5785 NC_003888 SC3C8.22c, unknown, len: 126 aa 1 4 14 17 [Reporter]SCO2065 (6A9)_PCR/6349 [Gene]SCO2065/5784 NC_003888 SC4G6.34, unknown, len: 446 aa; weakly similar to two other hypothetical proteins from Streptomyces coelicolor; TR:CAB45208 (EMBL:AL079308) (509 aa) fasta scores; opt: 382, z-score: 443.7, E(): 2.2e-17, (33.3% identity in 448 aa overlap) and TR:O86499 (EMBL:AL031232) (469 aa) fasta scores; opt: 222, z-score: 259.2, E(): 4.1e-07, (27.7% identity in 437 aa overlap) 1 4 14 16 [Reporter]SCO5433 (7M5)_PCR/6348 [Gene]SCO5433/5783 NC_003888 SC6A11.09, possible AfsR-like regulator, len: 816 aa; similar to others from Streptomyces spp. e.g. SW:P25941 (AFSR_STRCO) regulatory protein AfsR from Streptomyces coelicolor (993 aa) fasta scores: opt: 490, z-score: 458.0, E(): 4.2e-18, 33.7% identity in 656 aa overlap. Note that this protein contains a Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) but no helix-turn-helix motifs 1 4 14 15 [Reporter]SCO7241 (8I5)_PCR/6347 [Gene]SCO7241/5782 NC_003888 SC7A12.08, possible oxidoreductase, len: 540 aa. Similar to many including: Streptomyces griseus SW:CRTI_STRGR(EMBL:L37405) phytoene dehydrogenase (EC 1.3.-.-) (507 aa), fasta scores opt: 175 z-score: 191.7 E(): 0.0033 27.0% identity in 529 aa overlap and Mycobacterium tuberculosis SW:Y897_MYCTU(EMBL:Z73101) hypothetical 56.2 kda protein (535 aa), fasta scores opt: 1633 z-score: 1765.0 E():0 52.0% identity in 527 aa overlap. Contains a Pfam match to entry PF02032 Phytoene_dh, Phytoene dehydrogenase related enzyme. 1 4 14 14 [Reporter]SCO7724 (9E5)_PCR/6346 [Gene]SCO7724/5781 NC_003888 SC8D11.15c, hypothetical protein, len: 471 aa; similar to TR:Q9WX25 (EMBL:AL079345) Streptomyces coelicolor putative membrane protein SCE68.06c, 464 aa; fasta scores: opt: 428 z-score: 361.4 E(): 1.4e-12; 40.4% identity in 478 aa overlap 1 4 14 13 [Reporter]SCO5130 (10A5)_PCR/6345 [Gene]SCO5130/5780 NC_003888 SC9E12.15, possible ABC transporter integral membrane protein, len: 246 aa; similar to TR:CAB88184 (EMBL:AL352972) Streptomyces coelicolor putative ABC transporter integral membrane protein SCC30.14, 238 aa; fasta scores: opt: 516 z-score: 611.2 E(): 1.4e-26; 35.9% identity in 237 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 14 12 [Reporter]SCO2349 (11M1)_PCR/6344 [Gene]SCO2349/5779 NC_003888 SCC8A.07, unknown, len: 560 aa; similar to TR:O52813 (EMBL:AJ223998) hypothetical protein from Amycolatopsis orientalis (508 aa) fasta scores; opt: 1020, z-score: 1115.5, E(): 0, 48.0% identity in 542 aa overlap. 1 4 14 11 [Reporter]SCO4486 (12I1)_PCR/6343 [Gene]SCO4486/5778 NC_003888 SCD69.06, hypothetical protein, len: 135 aa; similar to TR:Q9Z549 (EMBL:AL035212) Streptomyces coelicolor putative quinone protein SC9B2.10c, 130 aa; fasta scores: opt: 191 z-score: 257.8 E(): 6.8e-07; 34.6% identity in 127 aa overlap and to TR:Q98IY9 (EMBL:AP002999) Rhizobium loti (Mesorhizobium loti) MLR2185 protein, 133 aa; fasta scores: opt: 522 Z-score: 639.6 E(): 5.4e-28; 64.122% identity in 131 aa overlap 1 4 14 10 [Reporter]SCO2854 (13E1)_PCR/6342 [Gene]SCO2854/5777 NC_003888 SCE20.28, possible transmembrane efflux protein, len: 477 aa. Similar to many including: Mycobacterium smegmatis TR:Q50392(EMBL:U40487) proton antiporter efflux pump (504 aa), fasta scores opt: 626 z-score: 586.9 E(): 2.6e-25 32.1% identity in 471 aa overlap and Streptomyces coelicolor TR:Q9ZBW5(EMBL:AL034443) putative integral membrane efflux protein SC4B5.03C (504 aa), fasta scores opt: 729 z-score: 681.9 E(): 1.3e-30 36.6% identity in 435 aa overlap. Contains a Prosite hit to PS00216 Sugar transport proteins signature 1 and multiple possible membrane spanning hydrophobic domains. 1 4 14 9 [Reporter]SCO0846 (14A1)_PCR/6341 [Gene]SCO0846/5776 NC_003888 SCF43A.36, hypothetical protein, len: 266 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 1 4 14 8 [Reporter]SCO1899 (14M21)_PCR/6340 [Gene]SCO1899/5775 NC_003888 SCI7.17, possible integral membrane sugar transport protein, len: 314aa; similar to many eg. TR:O30832 (EMBL:AF018073) sorbitol/mannitol transport inner membrane protein from Rhodobacter sphaeroides (290 aa) fasta scores; opt: 722, z-score: 829.0, E(): 0, (40.6% identity in 276 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible membrane spanning hydrophobic regions. 1 4 14 7 [Reporter]SCO0555 (15I21)_PCR/6339 [Gene]SCO0555/5774 NC_003888 SCF73.02c, possible membrane-bound oxidoreductase, len: 531 aa; some similarity to eukaryotic molybdenum-containing oxidoreductases e.g. SUOX_RAT Q07116 sulfite oxidase precursor (488 aa), fasta scores; opt: 262 z-score: 281.9 E(): 2.5e-08, 30.4% identity in 319 aa overlap, and NIA1_ORYSA nitrate reductase 1 (916 aa), fasta scores; opt: 247 z-score: 262.1 E(): 3.1e-07, 28.9% identity in 336 aa overlap. Contains several membrane spanning hydrophobic domains near N-terminus, and Pfam match to entry PF00174 oxidored_molyb, Oxidoreductase molybdopterin binding domain 1 4 14 6 [Reporter]SCO0694 (16E21)_PCR/6338 [Gene]SCO0694/5773 NC_003888 SCF42.04, unknown, len: 62 aa. 1 4 14 5 [Reporter]SCO2942 (17A21)_PCR/6337 [Gene]SCO2942/5772 NC_003888 SCE59.01c, possible oxidoreductase (partial), len: >116 aa; similar to N-terminal region of TR:Q9ZBN9 (EMBL:AL034492) Streptomyces coelicolor putative oxidoreductase subunit SC6C5.06, 296 aa; fasta scores: opt: 223 z-score: 268.2 E(): 1.6e-07; 37.6% identity in 93 aa overlap and to TR:P95637 (EMBL:U65440) Rhodopseudomonas palustris 4-hydroxybenzoyl-CoA reductase HbaD subunit, 327 aa; fasta scores: opt: 193 z-score: 233.2 E(): 1.4e-05; 42.6% identity in 94 aa overlap,SCE56.06, possible oxidoreductase (partial), len: >221 aa; similar of TR:Q9ZBN9 (EMBL:AL034492) Streptomyces coelicolor putative oxidoreductase subunit SC6C5.06, 296 aa; fasta scores: opt: 393 z-score: 443.2 E(): 2.8e-17; 34.7% identity in 222 aa overlap and to TR:P95637 (EMBL:U65440) Rhodopseudomonas palustris 4-hydroxybenzoyl-CoA reductase HbaD subunit, 327 aa; fasta scores: opt: 159 z-score: 184.7 E(): 0.0071; 32.1% identity in 184 aa overlap 4 2 20 15 [Reporter]SCO5230 (8D15)_PCR/3960 [Gene]SCO5230/3599 NC_003888 SC7E4.27c, possible integral membrane protein, len: 94 aa. Contains possible hydrophobic membrane spanning regions 1 4 14 4 [Reporter]SCO4035 (18M17)_PCR/6336 [Gene]SCO4035/5771 NC_003888 2SC10A7.39c, sigF, RNA polymerase sigma factor (fragment), len: >31 aa; identical to previously sequenced SW:RPOF_STRCO (EMBL:L11648) Streptomyces coelicolor RNA polymerase sigma factor SigF or RpoX, 278 aa,2SCD60.01c, sigF, RNA polymerase sigma factor, len: 287 aa; identical to previously sequenced SW:RPOF_STRCO (EMBL:L11648) Streptomyces coelicolor RNA polymerase sigma factor sigma-F SigF or RpoX, 287 aa. Contains Pfam match to entry PF00140 sigma70, Sigma-70 factor and match to Prosite entry PS00715 Sigma-70 factors family signature 1. Also contains possible helix-turn-helix motif at residues 252..273 (+6.22 SD) 4 2 20 13 [Reporter]SCO2579 (10L11)_PCR/3958 [Gene]SCO2579/3598 NC_003888 SCC123.17c, probable nicotinate-nucleotide adenylyltransferase, len: 238 aa. Highly similar in parts to several proteins of undefined function e.g. Mycobacterium tuberculosis TR:O86328 (EMBL:Z81368) hypothetical 23.1 KD protein (211 aa), fasta scores opt: 855 z-score: 945.6 E():0 66.5% identity in 194 aa overlap. Also C-terminal region similar to SW:NADD_ECOLI (EMBL:U23163) Escherichia coli nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18) NadD 213 aa; fasta scores: opt: 284 Z-score: 320.5 E(): 3.2e-10; 30.769% identity in 208 aa overlap 1 4 14 3 [Reporter]SCO4986 (19I17)_PCR/6335 [Gene]SCO4986/5770 NC_003888 2SCK36.09c, probable D-aminoacylase, len: 536 aa; similar to SW:NDAD_ALCXX (EMBL:S80683) Alcaligenes xylosoxydans D-aminoacylase (EC 3.5.1.81) Dan, 483 aa; fasta scores: opt: 370 Z-score: 413.6 bits: 86.1 E(): 2e-15; 33.647% identity in 532 aa overlap 4 2 20 12 [Reporter]SCO2601 (11H11)_PCR/3957 [Gene]SCO2601/3597 NC_003888 SCC88.12c, possible integral membrane protein, len: 358 aa. Contain possible hydrophobic membrane spanning regions 4 2 20 11 [Reporter]SCO4175 (12D11)_PCR/3956 [Gene]SCO4175/3596 NC_003888 SCD66.12c, hypothetical protein, len: 130 aa; similar to TR:CAB69732 (EMBL:AL137166) Streptomyces coelicolor hypothetical 7.8 kD protein, 73 aa; fasta scores: opt: 180 z-score: 223.4 E(): 5.7e-05; 43.7% identity in 71 aa overlap 4 2 20 10 [Reporter]SCO3092 (13P7)_PCR/3955 [Gene]SCO3092/3595 NC_003888 SCE41.01c, probable oxidoreductase (fragment), len: >317 aa; similar to SW:DHNA_ECOLI (EMBL:V00306) Escherichia coli NADH dehydrogenase (EC 1.6.99.3) Ndh, 433 aa; fasta scores: opt: 361 z-score: 417.8 E(): 9.1e-16; 27.6% identity in 330 aa overlap and to TR:CAB92372 (EMBL:AL356612) Streptomyces coelicolor putative NADH dehydrogenase SCD72A.05, 442 aa; fasta scores: opt: 1185 z-score: 1267.3 E(): 0; 57.0% identity in 321 aa overlap. Contains Pfam match to entry PF00070 pyr_redox, Pyridine nucleotide-disulphide oxidoreductase,SCE25.33c, possible dehydrogenase (fragment), len: >162 aa; similar to TR:Q9ZC17 (EMBL:AL033505) Streptomyces coelicolor putative dehydrogenase SC1E6.05, 629 aa; fasta scores: opt: 209 z-score: 253.8 E(): 1.1e-06; 32.6% identity in 141 aa overlap and to C-terminal region of SW:DHNA_ECOLI (EMBL:V00306) Escherichia coli NADH dehydrogenase (EC 1.6.99.3) Ndh, 433 aa; fasta scores: opt: 116 z-score: 145.6 E(): 1.2; 26.8% identity in 82 aa overlap 4 2 20 9 [Reporter]SCO0918 (14L7)_PCR/3954 [Gene]SCO0918/3594 NC_003888 SCM10.06, probable excinuclease ABC subunit A, len: 781 aa; similar to SW:UVRA_PSELE (EMBL:Z22595) Pseudomonas lemoignei putative excinuclease ABC subunit A (fragment) UvrA, 689 aa; fasta scores: opt: 1263 z-score: 1326.5 E(): 0; 57.5% identity in 670 aa overlap, to SW:UVRA_MICLU (EMBL:X15867) Micrococcus luteus excinuclease ABC subunit A UvrA, 992 aa; fasta scores: opt: 1096 z-score: 1149.1 E(): 0; 40.9% identity in 962 aa overlap and to S. coelicolor SC5F2A.02c, 752 aa; fasta scores: opt: 1207 z-score: 981.3 E(): 0; 44.8% identity in 772 aa overlap. Contains two Pfam matches to entry PF00005 ABC_tran, ABC transporter, two matches to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and another two to Prosite entry PS00211 ABC transporters family signature 4 2 20 8 [Reporter]SCO1745 (15H7)_PCR/3953 [Gene]SCO1745/3593 NC_003888 SCI11.34, probable two-component system response regulator, len: 221 aa; similar to SW:NARL_ECOLI (EMBL:X13360), NarL, Escherichia coli nitrate/nitrite response regulator protein (216 aa), fasta scores; opt: 482 z-score: 568.4 E(): 2.5e-24, 38.9% identity in 211 aa overlap and TR:Q53894 (EMBL:AL035654), AbsA2, S.coelicolor putative two-component system response regulator (222 aa) (43.1% identity in 218 aa overlap). Contains probable helix-turn-helix motif at aa 171-192 (Score 1021, +2.66 SD). Contains Pfam matches to entry PF00072 response_reg, Response regulator receiver domain and to entry PF00196 GerE, Bacterial regulatory proteins, luxR family. Contains PS00622 Bacterial regulatory proteins, luxR family signature 4 2 20 7 [Reporter]SCO1554 (16D7)_PCR/3952 [Gene]SCO1554/3592 NC_003888 SCL11.10c, putative nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, len: 612 aa; N-terminal region similar to TR:O07233 (EMBL:Z96800) Mycobacterium tuberculosis hypothetical 24.9 kD protein, 223 aa; fasta scores: opt: 558 z-score: 594.0 E(): 1.1e-25; 42.3% identity in 222 aa overlap and C-terminal region similr to SW:COBT_ECOLI (EMBL:U33333) Escherichia coli nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21) CobT, 359 aa; fasta scores: opt: 793 z-score: 837.6 E(): 0; 38.3% identity in 347 aa overlap. Contains Pfam match to entry PF00881 Nitroreductase, Nitroreductase family 4 2 20 6 [Reporter]SCO1163 (17P3)_PCR/3951 [Gene]SCO1163/3591 NC_003888 SCG8A.17c, unknown, len: 299 aa; region 230-290aa similar to region from several eg. TR:Q22551 (EMBL:U41746) T18H9.1 from Caenorhabditis elegans (586 aa) fasta scores; opt: 110, z-score: 134.9, E(): 4.5, 29.5% identity in 105 aa overlap and TR:P72390 (EMBL:U51332) hypothetical protein from Streptomyces coelicolor (122 aa) fasta scores; opt: 101, z-score: 134.5, E(): 4.8, 40.0% identity in 75 aa overlap. 4 2 20 5 [Reporter]SCO1450 (18L3)_PCR/3950 [Gene]SCO1450/3590 NC_003888 SCL6.07, probable uracyl permease, len: 478 aa; similar to TR:BAA35783 (EMBL:D90738) Escherichia coli uracil transport protein PyrP, 442 aa; fasta scores: opt: 908 z-score: 949.9 E(): 0; 43.1% identity in 429 aa overlap and to SW:PYRP_BACCL (EMBL:X76083) Bacillus caldolyticus uracil permease (uracil transporter) PyrP, 432 aa; fasta scores: opt: 770 z-score: 806.6 E(): 0; 33.0% identity in 437 aa overlap. Contains Pfam match to entry PF00860 xan_ur_permease, Xanthine/uracil permeases family. Contains also possible hydrophobic membrane spanning regions 1 4 14 2 [Reporter]SCO5059 (20A17)_PCR/6334 [Gene]SCO5059/5769 NC_003888 SCBAC20F6.02, ppgK, polyphosphate glucokinase, len: 246 aa; highly similar to SW:PPGK_MYCTU (EMBL:U44834) Mycobacterium tuberculosis polyphosphate glucokinase (EC 2.7.1.63) PpgK or RV2702 or MTCY05A6.23, 265 aa; fasta scores: opt: 912 Z-score: 1042.8 bits: 200.5 E(): 1.7e-50; 57.322% identity in 239 aa overlap. Contains Pfam match to entry PF00480 ROK, ROK family 1 4 13 22 [Reporter]SCO1278 (1A13)_PCR/6332 [Gene]SCO1278/5768 NC_003888 2SCG18.25, possible ATP/GTP-binding protein, len: 525 aa; similar to TR:O27682 (EMBL:AE000923) Methanobacterium thermoautotrophicum ABC transporter MTH1645, 561 aa; fasta scores: opt: 540 z-score: 600.5 E(): 5.8e-26; 30.1% identity in 372 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 4 13 21 [Reporter]SCO5739 (2M9)_PCR/6331 [Gene]SCO5739/5767 NC_003888 SC9A10.03, dapB, dihydrodipicolinate reductase, len: 250 aa; highly similar to many e.g. DAPB_CORGL dihydrodipicolinate reductase (EC 1.3.1.26) (248 aa), fasta scores; opt: 946 z-score: 1333.7 E(): 0, 56.1% identity in 246 aa overlap. Contains PS01298 Dihydrodipicolinate reductase signature 1 4 13 20 [Reporter]SCO3023 (3I9)_PCR/6330 [Gene]SCO3023/5766 NC_003888 SCE34.04c, sahH, adenosylhomocysteinase, len: 485 aa; highly similar to SW:SAHH_TRIVA (EMBL:U40872) Trichomonas vaginalis adenosylhomocysteinase (EC 3.3.1.1) SahH, 486 aa; fasta scores: opt: 2114 z-score: 2420.8 E(): 0; 65.2% identity in 477 aa overlap. Contains Pfam match to entry PF00670 AdoHcyase, S-adenosyl-L-homocysteine hydrolase and matches to Prosite entries PS00738 S-adenosyl-L-homocysteine hydrolase signature 1 and PS00739 S-adenosyl-L-homocysteine hydrolase signature 2 1 4 13 19 [Reporter]SCO5540 (4E9)_PCR/6329 [Gene]SCO5540/5765 NC_003888 SC1C2.21c, cvnA2, unknown, len: 1111 aa; contains two possible transmembrane domains at around 100 and 400 aa, a Gln-rich region at around 850 aa and a probable coiled-coil domain from 297 to 327. Overall 35.6% identical to upstream gene SC1C2.25c, but with much higher similarity between aa 477 and 719 1 4 13 18 [Reporter]SCO6390 (5A9)_PCR/6328 [Gene]SCO6390/5764 NC_003888 SC3C8.09, probable integral membrane protein, len: 367 aa; similar to a hypothetical protein from M. tuberculosis Y07S_MYCTU MTCY02B10.28c (653 aa), fatsta scores; opt: 144 z-score: 314.2 E(): 2.9e-10, 30.6% identity in 281 aa overlap. Also similar to S. coelicolor SC7H1.03c (E(): 3e-18, 35.2% identity in 355 aa overlap) 1 4 13 17 [Reporter]SCO5813 (6M5)_PCR/6327 [Gene]SCO5813/5763 NC_003888 SC5B8.03, unknown, len: 212 aa; highly Proline rich in N-terminus 1 4 13 16 [Reporter]SCO5429 (7I5)_PCR/6326 [Gene]SCO5429/5762 NC_003888 SC6A11.05c, possible integral membrane transport protein, len: 313 aa; similar to many proposed transport proteins e.g. TRNEW:CAB55533(EMBL:AL117322) putative integral membrane transport protein from Streptomyces coelicolor (317 aa) fasta scores; opt: 561, z-score: 649.2, E(): 9.4e-29, 34.4% identity in 314 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and possible membrane-spanning hydrophobic regions 4 2 20 4 [Reporter]SCO4904 (19H3)_PCR/3949 [Gene]SCO4904/3589 NC_003888 2SCK8.30c, possible integral membrane protein, len: 183 aa; similar to TR:CAC14351 (EMBL:AL445945) Streptomyces coelicolor putative integral membrane protein 2SCF60.18, 179 aa; fasta scores: opt: 193 z-score: 230.8 E(): 2.7e-05; 32.0% identity in 153 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 13 14 [Reporter]SCO7720 (9A5)_PCR/6324 [Gene]SCO7720/5761 NC_003888 SC8D11.11, unknown, len: 368 aa 4 2 20 3 [Reporter]SCP1.108 (20D3)_PCR/3948 [Gene]SCP1.108/3588 NC_003888 SCP1.108, unknown, len: 201aa; 1 4 13 13 [Reporter]SCO6251 (10M1)_PCR/6323 [Gene]SCO6251/5760 NC_003888 SCAH10.16, possible reductase, len: 185 aa; similar to TR:Q9ZGD0 (EMBL:AF080235) Streptomyces cyanogenus reductase homologue LanO, len: 193 aa; fasta scores: opt: 398 z-score: 505.3 E(): 9e-21; 41.0% identity in 188 aa overlap 4 2 20 2 [Reporter]SCO5082 (20L23)_PCR/3947 [Gene]SCO5082/3587 NC_003888 SCBAC28G1.08c, actII-1, probable transcriptional regulatory protein, len: 259 aa; identical to previously sequenced TR:Q53901 (EMBL:M64683) Streptomyces coelicolor ORF1-4 (ActII), 259 aa. Contains Pfam matches to entries PF00440 tetR, Bacterial regulatory proteins, tetR family and PF02909 tetR_C, Tetracyclin repressor, C-terminal all-alpha domain and possible helix-turn-helix motif at residues 53..73 (+2.78 SD) 4 2 19 22 [Reporter]SCO1348 (1L19)_PCR/3945 [Gene]SCO1348/3586 NC_003888 2SCG61.30c, unknown, len: 298aa; 4 2 19 21 [Reporter]SCO6501 (2H19)_PCR/3944 [Gene]SCO6501/3585 NC_003888 SC1E6.10, gvpF, probable gas vesicle synthesis protein, len: 257 aa; some similarity to GVF2_HALSA GVPF protein, chromosomal (217 aa), fasta scores; opt: 143 z-score: 191.0 E(): 0.0023, 27.1% identity in 247 aa overlap 4 2 19 20 [Reporter]SCO7656 (3D19)_PCR/3943 [Gene]SCO7656/3584 NC_003888 SC10F4.29, unknown, len: 173 aa 4 2 19 19 [Reporter]SCO7221 (4P15)_PCR/3942 [Gene]SCO7221/3583 NC_003888 SC2H12.20c, possible polyketide synthase, len: 389 aa. Weakly similar to several proteins from Eukaryotes and Prokaryotes involved in secondary metabolism e.g. Hydrangea macrophylla SW:CHSY_HYDMC(EMBL:AB011467) chalcone synthase (EC 2.3.1.74) a secondary plant metabolite (389 aa), fasta scores opt: 294 z-score: 333.6 E(): 4.1e-11 28.4% identity in 282 aa overlap and Pseudomonas fluorescens TR:Q51725(EMBL:U41818) putative polyketide synthase PhlD (349 aa), fasta scores opt: 433 z-score: 489.6 E(): 8.5e-20 26.4% identity in 386 aa overlap. Contains a Pfam match to entry PF00195 Chal_stil_synt, Chalcone and stilbene synthases. 4 2 19 18 [Reporter]SCO7683 (5L15)_PCR/3941 [Gene]SCO7683/3582 NC_003888 SC4C2.18, probable non-ribosomal peptide synthase, len: 1842 aa; similar to C-terminal domain of TR:AAG02364 (EMBL:AF210249) Streptomyces verticillus peptide synthetase NRPS2-1 BlmIV, 2626 aa; fasta scores: opt: 2514 z-score: 2613.8 E(): 0; 40.7% identity in 1287 aa overlap, to TR:Q9RFM7 (EMBL:AF184622) Pseudomonas aeruginosa pyochelin synthetase PchF, 1809 aa; fasta scores: opt: 2279 z-score: 2370.9 E(): 0; 39.7% identity in 1887 aa overlap, to TR:CAA18919 (EMBL:AL023496) Streptomyces coelicolor probable peptide synthase SC1A6.21, 1407 aa; fasta scores: opt: 1826 z-score: 1476.6 E(): 0; 36.7% identity in 1315 aa overlap and to C-terminal domain of Streptomyces coelicolor SC4C2.17, 2229 aa; fasta scores: opt: 1742 z-score: 1405.5 E(): 0; 43.0% identity in 997 aa overlap. Contains Pfam matches to entries PF00668 DUF4, Domain of unknown function DUF4, PF00501 AMP-binding, AMP-binding enzyme, PF00550 pp-binding, Phosphopantetheine attachment site and PF00975 Thioesterase, Thioesterase domain and matches to Prosite entries PS00455 Putative AMP-binding domain signature and PS00012 Phosphopantetheine attachment site 4 2 19 17 [Reporter]SCO7775 (6H15)_PCR/3940 [Gene]SCO7775/3581 NC_003888 SC5E9.23, possible secreted protein, len: 188aa; similar to regions from others eg. TR:Q9X583 (EMBL:AF121864) xylanase-arabinofuranosidase bifunctional enzyme from Streptomyces chattanoogensis (819 aa) fasta scores; opt: 198, z-score: 237.6, E(): 9.1e-06, 29.4% identity in 177 aa overlap. Contains possible N-terminal signal sequence and Pfam match to entry PF00652 Ricin_B_lectin, Similarity to lectin domain of ricin beta-chain. Similar to neighbouring CDS SC5E9.22 fasta scores; opt: 371, z-score: 401.0, E(): 1.1e-16, 42.2% identity in 173 aa overlap. 4 2 19 16 [Reporter]SCO6169 (7D15)_PCR/3939 [Gene]SCO6169/3580 NC_003888 SC6C5.05c, possible regulatory protein, len: 569aa; C-terminal similarity to TR:Q00509 (EMBL:X63451) SrmR protein from Streptomyces ambofaciens (604 aa) fasta scores; opt: 203, z-score: 206.9, E(): 0.00031, (30.0% identity in 207 aa overlap). Contains helix-turn-helix motif from 523 to 544 (+2.73 SD). 1 4 13 12 [Reporter]SCO2345 (11I1)_PCR/6322 [Gene]SCO2345/5759 NC_003888 SCC8A.03, possible peptidodoglycan-binding membrane protein, len: 452 aa; contains Pfam match to entry PF01471 PG_binding_1, Putative peptidoglycan binding domain, score 35.30, E-value 1.4e-06 and possible membrane-spanning hydrophobic region. 1 4 13 11 [Reporter]SCO4449 (12E1)_PCR/6321 [Gene]SCO4449/5758 NC_003888 SCD6.27c, probable hydrolase, len: 299 aa; similar to TR:O87275 (EMBL:AF015628) Staphylococcus cohnii streptogramin B lactonase VgbB, 295 aa; fasta scores: opt: 855 z-score: 887.0 E(): 0; 44.5% identity in 283 aa overlap. Contains 5x DNA degenerate repeats:G/R)(T/A)(G/R)RR(P/H)VVH 1 4 13 10 [Reporter]SCO2850 (13A1)_PCR/6320 [Gene]SCO2850/5757 NC_003888 SCE20.24c, possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, len: 285 aa. Similar to many including: Mycobacterium tuberculosis TR:O53242 (EMBL:AL021287) possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (239 aa), fasta scores opt: 544 z-score: 651.1 E(): 7e-29 40.3% identity in 221 aa overlap and similar in parts to the C-terminus of Escherichia coli SW:HPCE_ECOLI (EMBL:X75028) 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-) (405 aa), fasta scores opt: 467 z-score: 556.6 E(): 1.3e-23 40.2% identity in 214 aa overlap. Also similar to Streptomyces coelicolor TR:CAB55708 (EMBL:AL117387) putative 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase SCF41.05 (292 aa), fasta scores opt: 1142 z-score: 1213.4 E():0 61.5% identity in 286 aa overlap. Contains a Pfam match to entry PF01557 FAA_hydrolase, Fumarylacetoacetate (FAA) hydrolase family. 1 4 13 9 [Reporter]SCO0631 (13M21)_PCR/6319 [Gene]SCO0631/5756 NC_003888 SCF56.15c, unknown, len: 60 aa 1 4 13 8 [Reporter]SCO3430 (14I21)_PCR/6318 [Gene]SCO3430/5755 NC_003888 SCE9.37, rpsN, probable 30S ribosomal protein S14, len: 101 aa; similar to many e.g. SW:RS14_ECOLI (EMBL:X01563), rpsN, Escherichia coli 30S ribosomal protein S14 (100 aa), fasta scores; opt: 299 z-score: 377.0 E(): 1.1e-13, 44.0% identity in 100 aa overlap. Contains Pfam match to entry PF00253 Ribosomal_S14, Ribosomal protein S14, score 103.10, E-value 1.5e-30 1 4 13 7 [Reporter]SCO1711 (15E21)_PCR/6317 [Gene]SCO1711/5754 NC_003888 SCI30A.32c, possible dehydrogenase, len: 747aa; similar to many e.g. TR:O53648 (EMBL:AL021928) hypothetical protein from Mycobacterium tuberculosis (762 aa) fasta scores; opt: 617, z-score: 625.8, E(): 1.6e-27, (29.9% identity in 762 aa overlap) and SW:FDHF_ECOLI formate dehydrogenase from Escherichia coli (715 aa) fasta scores; opt: 466, z-score: 473.3, E(): 4.9e-19, (24.0% identity in 716 aa overlap). 1 4 13 5 [Reporter]SCO0744 (17M17)_PCR/6315 [Gene]SCO0744/5753 NC_003888 SCF81.03, unknown, len: 121 aa; similar to TR:Q98BX4 (EMBL:AP003006) MLL5389 protein from Rhizobium loti (Mesorhizobium loti) (124 aa) fasta scores; opt: 363, Z-score: 452.3, E(): 1.5e-17, 43.750% identity (43.750% ungapped) in 112 aa overlap. 1 4 13 4 [Reporter]SCO6094 (18I17)_PCR/6314 [Gene]SCO6094/5752 NC_003888 SCBAC1A6.18c, probable transport system integral membrane protein, len: 258 aa; similar to SW:SSUC_BACSU (EMBL:Z93102) Bacillus subtilis putative aliphatic sulfonates transport permease protein SsuC, 276 aa; fasta scores: opt: 378 z-score: 422.1 E(): 6.2e-16; 28.5% identity in 263 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 4 2 19 15 [Reporter]SCO5752 (8P11)_PCR/3938 [Gene]SCO5752/3579 NC_003888 SC7C7.07, unknown, len: 493 aa; similar to many members of the UPF0004 family e.g. YLIG_ECOLI (441 aa), fasta scores; opt: 642 z-score: 947.7 E(): 0, 31.8% identity in 487 aa overlap. Contains PS01278 Uncharacterized protein family UPF0004 signature. Also similar to S. coelicolor SC4H2.08, fasta scores; E(): 9.1e-23, 30.3% identity in 445 aa overlap 1 4 13 3 [Reporter]SCO4064 (19E17)_PCR/6313 [Gene]SCO4064/5751 NC_003888 2SCD60.30c, unknown, len: 87 aa 4 2 19 14 [Reporter]SCO5397 (9L11)_PCR/3937 [Gene]SCO5397/3578 NC_003888 SC8F4.01c, large Ala/Glu-rich protein, len: 1326 aa; hydrophilic with strong potential for coiled-coil structure throughout. 1 4 13 2 [Reporter]SCO5072 (20M13)_PCR/6312 [Gene]SCO5072/5750 NC_003888 SCBAC20F6.15, ORF1 hydroxylacyl-CoA dehydrogenase, len: 307 aa; identical to previously sequenced TR:Q53926 (EMBL:X62373) Streptomyces coelicolor hydroxylacyl-CoA dehydrogenase ORF1, 307 aa. Contains Pfam matches to entries PF02737 3HCDH_N, 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain and PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain. Contains possible hydrophobic membrane spanning region 4 2 19 13 [Reporter]SCO6237 (10H11)_PCR/3936 [Gene]SCO6237/3577 NC_003888 SCAH10.02, possible regulatory protein, len: 160 aa; similar to TR:Q53897 (EMBL:X60316) Streptomyces coelicolor A3(2) ORFA in AbaA gene, len: 192 aa; fasta scores: opt: 245 z-score: 318.1 E(): 2.4e-10; 36.5% identity in 148 aa overlap and to TR:O86675 (EMBL:SC4G2) Streptomyces coelicolor SC4G2.02c, 196 aa; fasta scores: opt: 436 z-score: 440.2 E(): 3.4e-19; 51.4% identity in 138 aa overlap 4 2 19 12 [Reporter]SCO2593 (11D11)_PCR/3935 [Gene]SCO2593/3576 NC_003888 SCC88.04c, unknown, len: 329 aa 4 2 19 10 [Reporter]SCO3281 (13L7)_PCR/3933 [Gene]SCO3281/3575 NC_003888 SCE39.31c, unknown, len: 136aa; similar to TR:O68643 (EMBL:AF052749) hypothetical protein from Pseudomonas putida (109 aa) fasta scores; opt: 359, z-score: 415.3, E(): 8.1e-16, (41.3% identity in 109 aa overlap). 4 2 19 9 [Reporter]SCO0799 (14H7)_PCR/3932 [Gene]SCO0799/3574 NC_003888 SCF43.10c, unknown, len: 241 aa. Weakly similar to Mycobacterium tuberculosis TR:P96239(EMBL:Z83864) hypothetical 28.3 KD protein (258 aa), fasta scores opt: 270 z-score: 339.5 E(): 1.6e-11 33.5% identity in 272 aa overlap. 4 2 19 8 [Reporter]SCO0423 (15D7)_PCR/3931 [Gene]SCO0423/3573 NC_003888 SCF51A.01c, putative membrane transport protein, len: 765 aa. Similar to many Streptomyces coelicolor integral membrane proteins including:TR:Q53902 (EMBL; M64683) ORF1-4 (ACTII) (711 aa),fasta scores opt: 291 z-score: 295.3 E(): 4.3e-09 27.7% identity in 779 aa overlap, TR:Q9Z577 (EMBL; AL035569) SC8D9.14 (748 aa), fasta scores opt: 1021 z-score: 1028.4 E(): 0 40.6% identity in 773 aa overlap and TR:O88022 (EMBL; AL031107) SC5A7.16C (705 aa), fasta scores opt: 639 z-score: 645.0 E(): 1.4e-28 37.2% identity in 764 aa overlap. Contains multiple possible transmembrane hydrophobic domains. 4 1 1 16 [Reporter]SCO7306 (6L20)_PCR/1527 [Gene]SCO7306/1399 NC_003888 SC5F8.16c, regulatory protein, len: 83 aa. Highly similar to several e.g. Streptomyces coelicolor TR:CAB88918(EMBL:AL353862) regulatory protein, WhiB, essential for sporulation (87 aa), fasta scores opt: 214 z-score: 299.5 E(): 3.3e-09 45.1% identity in 71 aa overlap. 4 2 19 7 [Reporter]SCO3157 (16P3)_PCR/3930 [Gene]SCO3157/3572 NC_003888 SCE87.08, possible penicillin-binding protein (putative secreted protein), len: 591 aa. Similar to many including: Streptomyces clavuligerus TR:P72405 (EMBL:U56256) penicillin-binding protein PcbR (551 aa), fasta scores opt: 775 z-score: 731.0 E(): 0 36.5% identity in 526 aa overlap and Streptomyces coelicolor TR:CAB45214 (EMBL:AL079308) putative penicillin-binding protein SCH69.17 (490 aa), fasta scores opt: 234 z-score: 226.4 E(): 3.1e-05 26.2% identity in 416 aa overlap. Also similar to the neighbouring CDS SCE87.07 (541 aa), fasta scores opt: 653 z-score: 513.9 E(): 2.7e-23 45.1% identity in 550 aa overlap. Contains a Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and a possible N-terminal signal sequence. 4 1 1 15 [Reporter]SCO7415 (7H20)_PCR/1526 [Gene]SCO7415/1398 NC_003888 SC6D11.11, possible racemase, len: 385 aa. Weakly similar to many racemases including: Pseudomonas putida SW:MANR_PSEPU(EMBL:M19043) mandelate racemase (EC 5.1.2.2) (359 aa), fasta scores opt: 315 z-score: 373.7 E(): 2.1e-13 26.4% identity in 371 aa overlap and Streptomyces coelicolor TR:CAB55709(EMBL:AL117387) possible racemase, SCF41.06 (416 aa), fasta scores opt: 383 z-score: 452.6 E(): 8.7e-18 31.0% identity in 371 aa overlap. Contains a Pfam match to entry PF01188 MR_MLE, Mandelate racemase / muconate lactonizing enzyme family. 4 2 19 6 [Reporter]SCO3704 (17L3)_PCR/3929 [Gene]SCO3704/3571 NC_003888 SCH35.20c, possible substrate-binding transport protein, len: 269aa; similar to many egs. TR:O31229 (EMBL:Y10817) molybdate-binding periplasmic transport protein from Arthrobacter nicotinovorans (260 aa) fasta scores; opt: 613, z-score: 670.6, E(): 5e-30, (43.4% identity in 256 aa overlap) and SW:MODA_ECOLI molybdate-binding periplasmic transport protein from Escherichia coli (257 aa) fasta scores; opt: 163, z-score: 186.1, E(): 0.0049, (31.7% identity in 249 aa overlap). Contains possible N-terminal signal sequence. 4 1 1 14 [Reporter]SCO6880 (8D20)_PCR/1525 [Gene]SCO6880/1397 NC_003888 SC7F9.32c, possible lipoprotein, len: 480 aa. Highly similar to Streptomyces coelicolor TR:Q9X871(EMBL:AL049661) putative integral membrane protein, SCE134.16 (488 aa), fasta scores opt: 2046 z-score: 2266.5 E(): 0 63.3% identity in 479 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 4 2 19 5 [Reporter]SCO1770 (18H3)_PCR/3928 [Gene]SCO1770/3570 NC_003888 SCI51.10c, conserved hypothetical protein, len: 264 aa; unknown function, similar to hypothetical proteins from Mycobacterium leprae, Mycobacterium tuberculosis and Bacillus subtilis e.g. TR:O33208 (EMBL:Z98268) Mycobacterium tuberculosis hypothetical protein (278 aa), fasta scores; opt: 875 z-score: 760.8 E(): 0, 56.2% identity in 267 aa overlap 4 1 1 13 [Reporter]SCO6128 (9P16)_PCR/1524 [Gene]SCO6128/1396 NC_003888 SC9B2.15c, unknown, len: 77 aa; similar to three proteins of unknown function from Streptomyces coelicolor egs. TR:O86600 (EMBL:AL031514) (81 aa) fasta scores; opt: 283, z-score: 412.5, E(): 1.1e-15, (54.7% identity in 75 aa overlap) and TR:Q53896 (EMBL:X60316) ORFD from the AbaA locus (75 aa) fasta scores; opt: 117, z-score: 182.4, E(): 0.0071, (46.2% identity in 52 aa overlap). Contains a 2x20 residue degenerate direct repeat, WFKSSYSG*EGGNC*EVA*E. 4 1 1 12 [Reporter]SCO1943 (10L16)_PCR/1523 [Gene]SCO1943/1395 NC_003888 SCC54.03c, possible electron transport protein, len: 111 aa; similar to TR:O53492 (EMBL:AL021899) hypothetical protein from Mycobacterium tuberculosis (111 aa) fasta scores; opt: 204, z-score: 250.4, E(): 1.2e-06, (36.9% identity in 103 aa overlap). Contains PS00202 Rubredoxin signature. 4 1 1 11 [Reporter]SCO4351 (11H16)_PCR/1522 [Gene]SCO4351/1394 NC_003888 SCD19.06, probable DNA invertase, len: 170 aa; similar to SW:DNIV_BPP1 (EMBL:X01828) Bacteriophage P1 DNA-invertase (site-specific recombinase) Cin, 186 aa; fasta scores: opt: 471 z-score: 560.8 E(): 9.9e-24; 48.8% identity in 170 aa overlap. Contains Pfam match to entry PF00239 recombinase, Site-specific recombinases 4 1 1 10 [Reporter]SCO4269 (12D16)_PCR/1521 [Gene]SCO4269/1393 NC_003888 SCD95A.02c, unknown, len: 166 aa 4 1 1 9 [Reporter]SCO3458 (13P12)_PCR/1520 [Gene]SCO3458/1392 NC_003888 SCE46.15c possible integral membrane protein, len: 249 aa; similar to various hypothetical proteins, e.g. SW:Y305_SYNY3 (EMBL:D64005) Synechocystis sp. hypothetical 22.2 kD protein SLR0305, 209 aa; fasta scores: opt: 419 z-score: 450.0 E(): 1e-17; 39.8% identity in 161 aa overlap 4 1 1 8 [Reporter]SCO3695 (14L12)_PCR/1519 [Gene]SCO3695/1391 NC_003888 SCH35.29, possible secreted protein, len: 162aa; contains possible N-terminal signal sequence. 4 1 1 7 [Reporter]SCO0844 (15H12)_PCR/1518 [Gene]SCO0844/1390 NC_003888 SCF43A.34c, possible DNA-binding protein, len: 113 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains probable helix-turn-helix motif at aa 9-30 (Score 1062, +2.80 SD) 1 4 12 22 [Reporter]SCO1248 (1M9)_PCR/6310 [Gene]SCO1248/5749 NC_003888 2SCG1.23c, conserved hypothetical protein, len: 124 aa; similar to TR:Q9RJS4 (EMBL:AL132707) Streptomyces coelicolor hypothetical 14.1 kD protein, 127 aa; fasta scores: opt: 214 z-score: 293.3 E(): 7.2e-09; 38.5% identity in 122 aa overlap 1 4 12 21 [Reporter]SCO4928 (2I9)_PCR/6309 [Gene]SCO4928/5748 NC_003888 SCK13.20, cya, adenylate cyclase, len: 381 aa; identical to previously sequenced SW:CYAA_STRCO (EMBL:X74768) Streptomyces coelicolor adenylate cyclase (EC 4.6.1.1) Cya, 381 aa. Contains Pfam match to entry PF00211 guanylate_cyc, Adenylate and Guanylate cyclase catalytic domain 1 4 12 19 [Reporter]SCO5534 (4A9)_PCR/6307 [Gene]SCO5534/5747 NC_003888 SC1C2.15, possible secreted lyase, len: 776 aa; similar to hyaluronidase from several organisms e.g. Propionibacterium acnes TR:Q59634 (EMBL:U15927) hyaluronidase (EC 4.2.2.1) (hyaluronate lyase) (752 aa), fasta scores; opt: 407 z-score: 783.6 E(): 0, 31.5% identity in 781 aa overlap. Contains N-tertminal signal sequence 1 4 12 18 [Reporter]SCO6576 (5M5)_PCR/6306 [Gene]SCO6576/5746 NC_003888 SC3F9.11, unknown, len: 245 aa; sim ilar to another from Streptomyces coelicolor TR:Q9Z4Z1 (EMBL:AL035707) SCE29.09C (236 aa) fasta scores; opt: 416, Z-score: 467.6, E(): 2.1e-18, 45.455% identity (52.083% ungapped) in 220 aa overlap . 1 4 12 17 [Reporter]SCO6679 (6I5)_PCR/6305 [Gene]SCO6679/5745 NC_003888 SC5A7.29c, unknown, len: 56 aa 1 4 12 16 [Reporter]SCO7282 (7E5)_PCR/6304 [Gene]SCO7282/5744 NC_003888 SC5H1.10c, hypothetical protein, len: 160 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis, amino acid composition and the presence of a possible RBS; similar to TR:Q98C01 (EMBL:AP003006) Rhizobium loti (Mesorhizobium loti) MLL5357 protein, 156 aa; fasta scores: opt: 617 Z-score: 752.0 E(): 2.9e-34; 58.170% identity in 153 aa overlap. Contains probable helix-turn-helix motif at aa 104-125 (Score 1006, +2.61 SD) 1 4 12 15 [Reporter]SCO4736 (8A5)_PCR/6303 [Gene]SCO4736/5743 NC_003888 SC6G4.14, probable phospho-sugar mutase, len: 452 aa ; similar to many phosphomannomutases and phoshoglucomutase s; also similar to to MRSA_ECOLI MRSA protein (445 aa), fas ta scores; opt: 1131 z-score: 827.7 E(): 0, 44.8% identity in 455 aa overlap, and to H. pylori UREC_HELPY putative ure ase operon ureC protein (445 aa), fasta scores; opt: 970 z- score: 1202.7 E(): 0, 39.6% identity in 434 aa overlap. Con tains Pfam match to entry PF00408 PGM_PMM, Phosphoglucomuta se and phosphomannomutase, score 356.30, E-value 3.4e-103 1 4 12 14 [Reporter]SCO2644 (9M1)_PCR/6302 [Gene]SCO2644/5742 NC_003888 SC8E4A.14c, probable lysR-family transcriptional regulator, len: 300 aa; similar to TR:CAB62680 (EMBL:AL133422) Streptomyces coelicolor putative lysR-family transcriptional regulatory protein SCM1.22c, 301 aa; fasta scores: opt: 714 z-score: 790.0 E(): 0; 43.8% identity in 299 aa overlap and to TR:GLTC_BACSU (EMBL:M28509) Bacillus subtilis transcriptional regulatory protein GltC, 300 aa; fasta scores: opt: 376 z-score: 420.6 E(): 5.1e-16; 27.1% identity in 288 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein and possible helix-turn-helix motif at residues 19..40 (+4.89 SD) 4 2 19 4 [Reporter]SCO4879 (19D3)_PCR/3927 [Gene]SCO4879/3569 NC_003888 2SCK8.05, hypothetical protein, len: 443 aa; central region presents low similarity with TR:Q9K1M6 (EMBL:AE002366) Neisseria meningitidis hypothetical protein NMB0065, 308 aa; fasta scores: opt: 341 z-score: 354.4 E(): 3.6e-12; 26.6% identity in 334 aa overlap 1 4 12 13 [Reporter]SCO6247 (10I1)_PCR/6301 [Gene]SCO6247/5741 NC_003888 SCAH10.12, possible allantoinase, len: 445 aa; similar SW:ALLA_YEAST (EMBL:M69294) Saccharomyces cerevisiae, allantoinase 460 aa; fasta scores: opt: 1232 z-score: 1441.5 E():0 ; 48.3% identity in 408 aa overlap and to SW:ALLA_BACSU (EMBL:Z99120) Bacillus subtilis putative allantoinase (EC 3.5.2.5) YunH, 446 aa; fasta scores: opt: 973 z-score: 1139.0 E(): 0; 36.5% identity in 444 aa overlap. Contains match to Pfam entry PF00744 Dihydrooratase, Dihydroorotase-like 4 2 19 3 [Reporter]SCP1.125 (19P23)_PCR/3926 [Gene]SCP1.125/3568 NC_003888 SCP1.125, unknown, len: 724aa; 1 4 12 12 [Reporter]SCO2706 (11E1)_PCR/6300 [Gene]SCO2706/5740 NC_003888 SCC61A.27, possible transferase, len: 436 aa; similar to many proposed to be involved in LPS biosynthesis e.g. SW:LPSB_RHIME (EMBL:AF193023) LpsB from lipopolysaccharide biosynthesis cluster of Rhizobium meliloti. Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1. Also contains a TTA encoded leucine close to the N-terminus, possible target for bldA regulation 4 2 19 2 [Reporter]SCO3966 (20H23)_PCR/3925 [Gene]SCO3966/3567 NC_003888 SCBAC25E3.03c, putative secreted protein, len: 216 aa: similar to many eg. TR:Q9RWR9 (EMBL:AE001918) conserved hypothetical protein from Deinococcus radiodurans (222 aa) fasta scores; opt: 329, Z-score: 384.3, 33.636% identity (37.949% ungapped) in 220 aa overlap. Contains Pfam match to entry PF02630 SCO1-SenC, SCO1/SenC. Contains possible N-terminal region signal peptide sequence 4 2 18 22 [Reporter]SCO1126 (1H19)_PCR/3923 [Gene]SCO1126/3566 NC_003888 2SCG38.19c, conserved hypothetical protein, len: 384 aa; similar to TR:Q9RVF3 (EMBL:AE001958) Deinococcus radiodurans cell wall synthesis protein, putative DR1076, 411 aa; fasta scores: opt: 327 z-score: 366.3 E(): 5.8e-13; 30.2% identity in 397 aa overlap 4 2 18 21 [Reporter]SCO5250 (2D19)_PCR/3922 [Gene]SCO5250/3565 NC_003888 2SC7G11.12, gtr, polyprenyl synthetase, len: 386 aa; previously sequenced as TR:Q9RGW1 (EMBL:AF104994) Streptomyces coelicolor A3(2) geranyl transferase Gtr, 386 aa. Contains Pfam match to entry PF00348 polyprenyl_synt, Polyprenyl synthetases and match to Prosite entry PS00723 Polyprenyl synthetases signature 1 4 2 18 20 [Reporter]SCO6820 (3P15)_PCR/3921 [Gene]SCO6820/3564 NC_003888 SC1A2.29c, possible oxidoreductase, len: 776 aa. Similar to a number of formate dehydrogenases e.g. Escherichia coli SW:FDHF_ECOLI(EMBL:M13563) formate dehydrogenase H (EC 1.2.1.2) (715 aa), fasta scores opt: 503 z-score: 564.0 E(): 5.2e-24 29.4% identity in 799 aa overlap. Contains Pfam matches to entries PF00384 molybdopterin, Prokaryotic molybdopterin oxidoreductases and PF01568 Molydop_binding, Molydopterin dinucleotide binding domain. 4 2 18 19 [Reporter]SCO7217 (4L15)_PCR/3920 [Gene]SCO7217/3563 NC_003888 SC2H12.16, possible iron transport protein, ATP-binding component, len: 258 aa. Highly similar to many e.g. Escherichia coli SW:FECE_ECOLI(EMBL:M26397) iron(iii) dicitrate transport ATP-binding protein, FecE (255aa), fasta scores opt: 616 z-score: 685.9 E(): 9.8e-31 43.1% identity in 246 aa overlap. Contains Prosite hits to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) and a Pfam match to entry PF00005 ABC_tran, ABC transporter. 4 1 1 6 [Reporter]SCO3402 (16D12)_PCR/1517 [Gene]SCO3402/1389 NC_003888 SCE9.09, possible secreted protein, len: 162 aa; unknown function, similar to TR:O06277 (EMBL:Z95557) Mycobacterium tuberculosis hypothetical protein (158 aa), fasta scores; opt: 174 z-score: 218.1 E(): 7.9e-05, 31.3% identity in 128 aa overlap. Hydrophobic protein containing possible N-terminal signal sequence 4 2 18 18 [Reporter]SCO2394 (5H15)_PCR/3919 [Gene]SCO2394/3562 NC_003888 SC4A7.22, putative secreted protein, len: 417 aa; similar to TR:Q9I783 (EMBL:AE004444) Pseudomonas aeruginosa hypothetical protein PA0049, 553 aa; fasta scores: opt: 566 Z-score: 609.9 E(): 2.4e-26; 35.800% identity in 500 aa overlap. Contains possible N-terminal region signal peptide sequence 4 1 1 5 [Reporter]SCO3342 (17P8)_PCR/1516 [Gene]SCO3342/1388 NC_003888 SCE7.09c, possible glycine-rich secreted protein, len: 224aa; Contains a possible N-terminal signal sequence. Also contains Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 4 2 18 17 [Reporter]SCO7770 (6D15)_PCR/3918 [Gene]SCO7770/3561 NC_003888 SC5E9.18, unknown, len: 191 aa; similar to a region from several predicted ATP/GTP-binding proteins eg. TR:O86768 (EMBL:AL031035) from Streptomyces coelicolor (886 aa) fasta scores; opt: 448, z-score: 543.2, E(): 8.6e-23, 44.2% identity in 172 aa overlap 4 1 1 4 [Reporter]SCO3148 (18L8)_PCR/1515 [Gene]SCO3148/1387 NC_003888 SCE66.27c, possible isopentenyl monophosphate kinase, len: 299 aa; similar to many e.g. SW:O05596 (IPK_MYCTU) isopentenyl monophosphate kinase from Mycobacterium tuberculosis (306 aa) fasta scores; opt: 925, z-score: 1022.2, E(): 0, 54.5% identity in 297 aa overlap and SW:P56848 (IPK_MENPI) isopentenyl monophosphate kinase from Mentha piperita (Peppermint) (405 aa) fasta scores; opt: 395, z-score: 439.8, E(): 5.1e-17, 33.6% identity in 265 aa overlap. 4 2 18 16 [Reporter]SCO5657 (7P11)_PCR/3917 [Gene]SCO5657/3560 NC_003888 SC6A9.10c, probable aldehyde dehydrogenase, len: 479 aa; similar to many e.g. DHAB_BACSU betaine aldehyde dehydrogenase (EC 1.2.1.8) (490 aa), fasta scores; opt: 1131 z-score: 1242.7 E(): 0, 39.2% identity in 472 aa overlap. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site, PS00070 Aldehyde dehydrogenases cysteine active site and Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenases, score 598.80, E-value 3.2e-176 4 1 1 3 [Reporter]SCP1.252c (19H8)_PCR/1514 [Gene]SCP1.252c/1386 NC_003888 SCP1.252c, unknown, len: 122176aa; 3 1 23 22 [Reporter]SCO4921 (1K24)_PCR/1512 [Gene]SCO4921/1385 NC_003888 SCK13.13c, accA2, putative acyl-CoA carboxylase complex A subunit, len: 590 aa; identical to previously sequenced TR:Q9X4K6 (EMBL:AF113603) Streptomyces coelicolor putative acyl-CoA carboxylase complex A subunit AccA2, 590 aa. Contains Pfam match to entry PF00289 CPSase_L_chain, Carbamoyl-phosphate synthase (CPSase) and PF00364 biotin_lipoyl, Biotin-requiring enzymes and match to Prosite entry PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00188 Biotin-requiring enzymes attachment site 3 1 23 21 [Reporter]SCO7000 (2G24)_PCR/1511 [Gene]SCO7000/1384 NC_003888 SC8F11.26c, idh, isocitrate dehydrogenase, len: 739 aa. Shares 97.8% sequence identity with that previously sequenced and characterised: Streptomyces coelicolor isocitrate dehydrogenase Idh, 741 aa TR:Q9X5M9(EMBL:AF127018) 3 1 23 20 [Reporter]SCO6330 (3C24)_PCR/1510 [Gene]SCO6330/1383 NC_003888 SC10H5.06, probable membrane protein, len: 207 aa; similar to S. coelicolor TR:O54192 SC7H1.33c (191 aa), fasta scores; opt: 312 z-score: 355.2 E(): 1.6e-12, 36.8% identity in 182 aa overlap 3 1 23 19 [Reporter]SCO6222 (4O20)_PCR/1509 [Gene]SCO6222/1382 NC_003888 SC2H4.04c, probable aminotransferase, len: 402 aa; smilar to many e.g. AAT_BACST aspartate aminotransferase (EC 2.6.1.1) (393 aa), fasta scores; opt: 657 z-score: 607.8 E(): 1.3e-26, 33.9% identity in 386 aa overlap. Contains Pfam match to entry PF00155 aminotran_1, Aminotransferases class-I, score 136.00, E-value 6.9e-37 3 1 23 18 [Reporter]SCO7041 (5K20)_PCR/1508 [Gene]SCO7041/1381 NC_003888 SC4G1.07, unknown, len: 136 aa. High content in alanine, leucine and aspartate amino acid residues 3 1 23 17 [Reporter]SCO7573 (6G20)_PCR/1507 [Gene]SCO7573/1380 NC_003888 SC5F1.27c, probable anti-sigma factor antagonist, len: 114 aa; similar to SW:RSBV_STRCO (EMBL:AF134889) Streptomyces coelicolor anti-sigma factor B antagonist BldG, 113 aa; fasta scores: opt: 320 z-score: 436.5 E(): 8.8e-17; 49.5% identity in 99 aa overlap. Contains Pfam match to entry PF01740 STAS, STAS domain 1 4 12 11 [Reporter]SCO4445 (12A1)_PCR/6299 [Gene]SCO4445/5739 NC_003888 SCD6.23c, possible transcriptional regulator, len: 327 aa; similar to TR:Q9XBC8 (EMBL:AL078635) Streptomyces coelicolor putative transcriptional regulator CZA382.23c, 335 aa; fasta scores: opt: 347 z-score: 400.5 E(): 7.5e-15; 36.7% identity in 270 aa overlap 1 4 12 10 [Reporter]SCO3530 (12M21)_PCR/6298 [Gene]SCO3530/5738 NC_003888 SCE2.11, unknown, len: 199 aa; similar to TR:Q9L1M9 (EMBL:AL138977) Streptomyces coelicolor hypothetical protein SC7F9.38, 211 aa; fasta scores: opt: 562 Z-score: 634.5 E(): 1e-27; 44.444% identity in 189 aa overlap 1 4 12 9 [Reporter]SCO0532 (13I21)_PCR/6297 [Gene]SCO0532/5737 NC_003888 SCF11.12, probable sugar transporter membrane protein, len: 302 aa; similar to TR:CAB55533 (EMBL:Y07706) Streptomyces coelicolor inner membrane protein MalF, 300 aa; fasta scores: opt: 493 z-score: 600.8 E(): 4.3e-26; 32.6% identity in 291 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, binding-protein-dependent transport systems inner membrane component and a prosite match to entry PS00402 binding-protein-dependent transport systems inner membrane component signature 1 4 12 8 [Reporter]SCO0086 (14E21)_PCR/6296 [Gene]SCO0086/5736 NC_003888 SCJ11.15c, possible ribosylglycohydrolase (putative secreted protein), len: 312 aa; similar to SW:DRAG_RHORU (EMBL:X16187) ADP-ribosylglycohydrolase DraG from Rhodospirillum rubrum (294 aa) fasta scores; opt: 267, z-score: 299.5, E(): 2.5e-09, (31.1% identity in 283 aa overlap) 1 4 12 7 [Reporter]SCO0275 (15A21)_PCR/6295 [Gene]SCO0275/5735 NC_003888 SCF85.03, possible transcriptional repressor protein, len: 385 aa. Low similarity to many repressor proteins e.g. Anaerocellum thermophilum SW:XYLR_ANATH (EMBL; Z69782)xylose repressor (399 aa), fasta scores opt: 325 z-score: 375.7 E(): 1.4e-13 23.3% identity in 395 aa overlap and Streptomyces coelicolor TR:O50502 (EMBL; AL009199) probable transcriptional repressor protein SC7B7.05 (403 aa), fasta scores opt: 402 z-score: 463.7 E(): 1.8e-18 29.7% identity in 404 aa overlap. Contains a Pfam match to entry PF00480 ROK, ROK family and has a possible helix turn helix motif between aa 30..51. 1 4 12 6 [Reporter]SCO1080 (16M17)_PCR/6294 [Gene]SCO1080/5734 NC_003888 SCG22.26c, hypothetical protein, len: 432 aa; C-terminal domain similar to TR:Q9ZBK3 (EMBL:AL035161) Streptomyces coelicolor putative lyase SC9C7.07c, 339 aa; fasta scores: opt: 163 z-score: 187.9 E(): 0.0054; 27.7% identity in 206 aa overlap 1 4 12 5 [Reporter]SCO0026 (17I17)_PCR/6293 [Gene]SCO0026/5733 NC_003888 SCJ4.07, possible secreted protein, len: 171 aa; Contains possible N-terminal signal sequence. Also contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 1 4 12 3 [Reporter]SCP1.116 (19A17)_PCR/6291 [Gene]SCP1.116/5732 NC_003888 SCP1.116, possible ECF-family sigma factor, len: 380aa; region similar to many eg. TR:Q9RIT1 (EMBL:AJ010584) ECF sigma factor from Streptomyces coelicolor (264 aa) fasta scores; opt: 485, z-score: 519.3, E(): 1.9e-21, 39.0% identity in 223 aa overlap and SW:P35165 (SIGX_BACSU) RNA polymerase sigma factor SigX from Bacillus subtilis (194 aa) fasta scores; opt: 215, z-score: 237.7, E(): 9e-06, 29.0% identity in 162 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily). Contains a TTA encoded leucine at residue 31, possible target for bldA regulation. 4 2 18 15 [Reporter]SCO5748 (8L11)_PCR/3916 [Gene]SCO5748/3559 NC_003888 SC7C7.03, probable sensory histidine kinase, len: 1829 aa; N-terminus has 11 92aa repeats. Highly similar to putative osmosensing histidine kinases from yeasts (with only five repeats) e.g. Neurospora crassa TR:Q01318 (EMBL:U53189) osmotic-1 locus putative histidine kinase (1298 aa), fasta scores; opt: 2011 z-score: 1764.6 E(): 0, 38.3% identity in 1281 aa overlap. Also similar to BARA_ECOLI sensor protein BarA (918 aa) (with only one copy of the repeat), fasta scores; opt: 378 z-score: 755.8, E(): 0, 28.9% identity in 619 aa overlap. Extreme N-terminus has similarity to the phosphorylated receiver domain of two-component regulators e.g. SW:YEDW_ECOLI (239 aa), blast scores; Expect = 3.1e-12, 32% identity in 115 aa overlap, and also to the downstream regulator SC7C7.04; 37.6% identity in 109 aa overlap. Contains probable coiled-coil from 1285 to 1364 and Pfam matches to entry signal PF00512, Signal C termial domain, score 230.38 and to entry response_reg PF00072, Response regulator receiver domain, score 146 1 4 12 2 [Reporter]SCO7478 (20I13)_PCR/6290 [Gene]SCO7478/5731 NC_003888 SCBAC17A6.11c, possible phosphotransferase, len: 300aa: similar to many some of which are antibiotic resistance determinants eg. TR:O05841 (EMBL:Z95120) hypothetical protein from Mycobacterium tuberculosis (474 aa) fasta scores; opt: 971, Z-score: 1047.4, 49.315% identity (50.000% ungapped) in 292 aa overlap and TR:Q53826 (EMBL:U13078) capreomycin phosphotransferase from Streptomyces capreolus (281 aa) fasta scores; opt: 162, Z-score: 183.1, 28.632% identity (30.734% ungapped) in 234 aa overlap. Contains Pfam match to entry PF01636 APH, Aminoglycoside phosphotransferase. 4 2 18 14 [Reporter]SCO5520 (9H11)_PCR/3915 [Gene]SCO5520/3558 NC_003888 SC1C2.01, probable delta-1-pyrroline-5-carboxylate dehydrogenase, partial CDS, len >408 aa; similar to many e.g. TR:O50443 (EMBL:AL010186) delta-1-pyrroline-5-carboxylate dehydrogenase (M. tuberculosis) (543 aa), fasta scores; opt: 1753 z-score: 2214.2 E(): 0, 65.1% identity in 407 aa overlap, and PUT2_HUMAN delta-1-pyrroline-5-carboxylate dehydrogenase (563 aa), fasta scores; opt: 1273 z-score: 1730.4 E(): 0, 48.5% identity in 408 aa overlap. Contains PS00687 and PS00070 Aldehyde dehydrogenases glutamic acid active site and cysteine active site and Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenases, score 114.70, E-value 1.8e-30,SC8D9.32, partial CDS, probable pyrroline-5-carboxylate dehydrogenase, len: >182aa; similar to many eg. TR:O50443 (EMBL:AL010186) RocA, probable pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis (543 aa) fasta scores; opt: 843, z-score: 1024.6, E(): 0, (66.5% identity in 182 aa overlap) and SW:PUT2_AGABI PruA, delta 1-pyrroline-5-carboxylate dehydrogenase from Agaricus bisporus (common mushroom) (546 aa) fasta scores; opt: 521, z-score: 634.9, E(): 4.7e-28, (44.5% identity in 182 aa overlap). 1 4 11 22 [Reporter]SCO1240 (1I9)_PCR/6288 [Gene]SCO1240/5730 NC_003888 2SCG1.15, possible NLP/P60 family protein (putative secreted protein), len: 157 aa; similar to C-terminal region of TR:CAB92659 (EMBL:AL356832) Streptomyces coelicolor putative NLP/P60 family secreted protein SCD63A.07c, 398 aa; fasta scores: opt: 351 z-score: 415.4 E(): 1.1e-15; 42.5% identity in 134 aa overlap. Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family. Contains possible N-terminal region signal peptide sequence 4 2 18 13 [Reporter]SCO3798 (10D11)_PCR/3914 [Gene]SCO3798/3557 NC_003888 SCAC2.06c, possible chromosome condensation protein, len: 325 aa; similar to many eukaryote Pirin-like proteins, e.g. TR:O00625 (EMBL:Y07868) Homo sapiens Pirin, 290 aa; fasta scores: opt: 353 z-score: 412.8 E(): 1.7e-15; 38.6% identity in 277 aa overlap. Contains match to Prosite entry PS00626 Regulator of chromosome condensation (RCC1) signature 2 and the actinophage PhiC31 left and right junctions attachment site, attB 4 2 18 12 [Reporter]SCO4392 (11P7)_PCR/3913 [Gene]SCO4392/3556 NC_003888 SCD10.24, possible esterase, len: 241 aa; similar to TR:Q9RBI4 (EMBL:AF150928) Acinetobacter sp. ADP1 alkyl salicylate esterase SalE, 239 aa; fasta scores: opt: 360 z-score: 411.1 E(): 2e-15; 28.6% identity in 241 aa overlap 4 2 18 11 [Reporter]SCO3962 (12L7)_PCR/3912 [Gene]SCO3962/3555 NC_003888 SCD78.29c, pheA, prephenate dehydratase, len: 310 aa, similar to many eg. PHEA_AMYME (EMBL:L47666) prephenate dehydratase from Amycolatopsis methanolica (304 aa), fasta scores; opt: 821, z-score: 1164.7, E(): 0, 46.9% identity in 305 aa overlap. Contains PS00857 Prephenate dehydratase signature 1 and Pfam match to entry PF00800 PDT, Prephenate dehydratase, score 354.80, E-value 9.5e-103. Note: Can participate in the biosynthesis of Phenylalanine, Tyrosine and Tryptophan 4 2 18 10 [Reporter]SCO3086 (13H7)_PCR/3911 [Gene]SCO3086/3554 NC_003888 SCE25.27, possible lipoprotein, len: 259 aa. Contains matches to Prosite entries PS00013 Prokaryotic membrane lipoprotein lipid attachment site (correctly situated) and PS00017 ATP/GTP-binding site motif A (P-loop), possible N-terminal region signal peptide sequence and high content in alanine and glycine amino acid residues 4 2 18 9 [Reporter]SCO0456 (14D7)_PCR/3910 [Gene]SCO0456/3553 NC_003888 SCF51A.34, possible LacI family transcriptional regulator, len: 347 aa. Highly similar to several including: Escherichia coli SW:CYTR_ECOLI (EMBL; X03683) transcriptional repressor CytR (341 aa), fasta scores opt: 504 z-score: 574.2 E(): 1.3e-24 31.6% identity in 339 aa overlap and Streptomyces coelicolor TR:O86598 (EMBL; AL031514) putative transcriptional regulator SC2H4.15 (342 aa), fasta scores opt: 807 z-score: 915.3 E(): 0 44.4% identity in 333 aa overlap. Contains a Pfam match to entry PF00356 lacI, Bacterial regulatory proteins, lacI family, a Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family and a possible helix-turn-helix motif between residues 13..34 (+5.95 SD). 3 1 23 16 [Reporter]SCO7410 (7C20)_PCR/1506 [Gene]SCO7410/1379 NC_003888 SC6D11.06c, possible binding-protein dependent transport protein, len: 308 aa. Similar to many other transport proteins e.g. Streptomyces coelicolor TR:CAB61178(EMBL:AL132973) putative binding protein dependent transport protein, SCF91.21 (328 aa), fasta scores opt: 622 z-score: 709.6 E(): 4.2e-32 35.9% identity in 315 aa overlap. Contains possible membrane spanning hydrophobic domains and a Prosite hit to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. 4 2 18 8 [Reporter]SCO0628 (15P3)_PCR/3909 [Gene]SCO0628/3552 NC_003888 SCF56.12c, possible secreted protein, len: 532 aa; similar to TR:Q55846 (EMBL:D64004) Synechocystis sp. hypothetical 76.4 kD protein, 684 aa; fasta scores: opt: 236 z-score: 266.6 E(): 1.8e-07; 27.3% identity in 662 aa overlap 3 1 23 15 [Reporter]SCO2012 (8O16)_PCR/1505 [Gene]SCO2012/1378 NC_003888 SC7H2.26, probable branched chain amino acid transport ATP-binding protein, len: 238aa; similar to many eg. SW:LIVF_ECOLI branched chain amino acid transport ATP-binding protein from Escherichia coli (237 aa) fasta scores; opt: 774, z-score: 860.8, E(): 0, (52.4% identity in 233 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. 4 2 18 7 [Reporter]SCO0492 (16L3)_PCR/3908 [Gene]SCO0492/3551 NC_003888 SCF34.11c, probable peptide synthetase, len: 3643 aa; similar to parts of many antibiotic peptide synthetases e.g. the C-terminal half of TR:Q9Z4X6 (EMBL:AL035640) Streptomyces coelicolor CDA peptide synthetase I (7463 aa), fasta scores; opt: 1948 z-score: 1929.3 E(): 0, 33.7% identity in 3723 aa overlap. Also similar to parts of putative peptide synthetases from the exochelin biosynthesis locus of Mycobacterium smegmatis TR:O87314 (EMBL:AF027770), FxbC, putative peptide synthetase (4976 aa) (42.3% identity in 3756 aa overlap). Contains 3 Pfam matches to entry PF00550 pp-binding, Phosphopantetheine attachment site, 3 Pfam matches to entry PF00501 AMP-binding, AMP-binding enzyme, 4 Pfam matches to entry PF00668 DUF4, Domain of unknown function, 2 PS00455 Putative AMP-binding domain signatures, 2 PS00012 Phosphopantetheine attachment sites and 1 PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 23 14 [Reporter]SCO6123 (9K16)_PCR/1504 [Gene]SCO6123/1377 NC_003888 SC9B2.10c, possible quinone binding protein, len: 130aa; similar to TR:O05205 (EMBL:U92563) mitomycin-binding protein from Streptomyces lavendulae (130 aa) fasta scores; opt: 453, z-score: 551.3, E(): 2.1e-23, (49.6% identity in 129 aa overlap). 4 2 18 6 [Reporter]SCO0863 (17H3)_PCR/3907 [Gene]SCO0863/3550 NC_003888 SCM2.16c, possible integral membrane protein, len: 485 aa. Weakly similar to Pseudomonas aeruginosa TR:P72122(EMBL:D28119) OprC outer membrane protein C (471 aa), fasta scores opt: 256 z-score: 271.9 E(): 9.1e-08 28.5% identity in 466 aa overlap and to TR:CAC44337 (EMBL:AL596102) Streptomyces coelicolor putative integral membrane protein SCBAC19F3.14, 517 aa; fasta scores: opt: 581 Z-score: 603.9 E(): 5.2e-26; 46.076% identity in 497 aa overlap. Contains possible membrane spanning hydrophobic domains 3 1 23 13 [Reporter]SCO2444 (10G16)_PCR/1503 [Gene]SCO2444/1376 NC_003888 SCC24.15, possible fatty acid synthase, len: 485 aa; similar to TR:Q9ZIP5 (EMBL:AF022387) Rhizobium trifolii malonyl CoA synthase MatB, 504 aa; fasta scores: opt: 814 z-score: 831.1 E(): 0; 38.0% identity in 502 aa overlap. Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and match to Prosite entry PS00455 Putative AMP-binding domain signature 3 1 23 12 [Reporter]SCO4346 (11C16)_PCR/1502 [Gene]SCO4346/1375 NC_003888 SCD19.01c, unknown(fragment), len: >119 aa. Contains two TTA leucine codons, possible targets for bldA regulation,SCD12A.29c, unknown (fragment), len: > 282 aa 3 1 23 11 [Reporter]SCO4229 (12O12)_PCR/1501 [Gene]SCO4229/1374 NC_003888 SCD8A.02, possible sensor kinase, len: 426 aa; similar to SW:SEX3_MYCTU (EMBL:Y13628) Mycobacterium tuberculosis sensor-like histidine kinase SenX3 (EC 2.7.3.-), 410 aa; fasta scores: opt: 874 z-score: 939.7 E(): 0; 43.6% identity in 406 aa overlap and to C-terminal region of SW:PHOR_ECOLI (EMBL:X04704) Escherichia coli phosphate regulon sensor protein PhoR, 431 aa; fasta scores: opt: 521 z-score: 562.5 E(): 6.7e-24; 36.8% identity in 261 aa overlap; also similar to various S. coelicolor sensor proteins, e.g. SCH22A.18c, 370 aa; fasta scores: opt: 415 z-score: 388.1 E(): 3.9e-16; 31.1% identity in 383 aa overlap. Contains Pfam match to entry PF00512 signal, Histidine kinase and possible N-terminal region signal peptide sequence 3 1 23 10 [Reporter]SCO3453 (13K12)_PCR/1500 [Gene]SCO3453/1373 NC_003888 SCE46.10c, probable ABC-transporter ATP binding protein, len: 354 aa; similar to TR:CAB49712 (EMBL:AJ248285) Pyrococcus abyssi ABC-transporter ATP binding protein, 329 aa; fasta scores opt: 708 z-score: 742.4 E(): 0; 43.9% identity in 280 aa overlap and to SW:CYSA_ECOLI (EMBL:M32101) Escherichia coli sulfate transport ATP-binding protein CysA, 365 aa; fasta scores: opt: 668 z-score: 700.3 E(): 1.2e-31; 38.6% identity in 332 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and two matches to Prosite entries PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 23 9 [Reporter]SCO1475 (14G12)_PCR/1499 [Gene]SCO1475/1372 NC_003888 SCL6.32c, probable primosomal protein n', len: 716 aa; similar to SW:PRIA_MYCTU (EMBL:Z80108) Mycobacterium tuberculosis putative primosomal protein n' (replication factor Y) PriA, 655 aa; fasta scores: opt: 2088 z-score: 2092.0 E(): 0; 51.9% identity in 669 aa overlap and to SW:PRIA_ECOLI (EMBL:M33293) Escherichia coli primosomal protein n' (replication factor Y) PriA, 732 aa; fasta scores: opt: 375 z-score: 377.6 E(): 1.3e-13; 25.1% identity in 553 aa overlap 3 1 23 8 [Reporter]SCO3481 (15C12)_PCR/1498 [Gene]SCO3481/1371 NC_003888 SCE65.17c unknown, len: 370 aa 3 1 23 7 [Reporter]SCO0766 (16O8)_PCR/1497 [Gene]SCO0766/1370 NC_003888 SCF81.25c, possible secreted beta-galactosidase, len: 1028 aa; similar to SW:BGA2_ECOLI (EMBL:M64441) Escherichia coli evolved beta-galactosidase alpha-subunit (EC 3.2.1.23) (lactase) 1031 aa; fasta scores: opt: 482 z-score: 519.9 E(): 1.4e-21; 30.6% identity in 464 aa overlap. Contains N-terminal signal peptide sequence 1 4 11 21 [Reporter]SCO5032 (2E9)_PCR/6287 [Gene]SCO5032/5729 NC_003888 SCK7.05c, ahpC, alkyl hydroperoxide reductase, len:184 aa; identical to previously seqeunced TR:Q9RN72 (EMBL:AF186371) Streptomyces coelicolor A3(2) AhpC, 184 aa. Contains Pfam match to entry PF00578 AhpC-TSA, AhpC/TSA family 1 4 11 20 [Reporter]SCO4141 (3A9)_PCR/6286 [Gene]SCO4141/5728 NC_003888 SCD84.08c, pstC, phosphate ABC transport system permease protein, len: 336 aa; similar to TR:O86344 (EMBL:Z47983) Mycobacterium tuberculosis phosphate transport system permease protein PstC2, 324 aa; fasta scores: opt: 900 z-score: 993.3 E(): 0; 49.4% identity in 308 aa overlap and to SW:PSTC_ECOLI (EMBL:X02723) Escherichia coli phosphate transport system permease protein PstC or PhoW, 319 aa; fasta scores: opt: 667 z-score: 738.3 E(): 0; 40.5% identity in 311 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 1 4 11 19 [Reporter]SCO6618 (4M5)_PCR/6285 [Gene]SCO6618/5727 NC_003888 SC1F2.15c, unknown, len: 226 aa 1 4 11 18 [Reporter]SCO6572 (5I5)_PCR/6284 [Gene]SCO6572/5726 NC_003888 SC3F9.07, possible glycosyl hydrolase (putative secreted protein), len: 1238 aa; similar to TR:Q9K473 (EMBL:AL359215) putative glycosyl hydrolase from Streptomyces coelicolor (962 aa) fasta scores; opt: 3614, Z-score: 3774.0, 63.899% identity (65.074% ungapped) in 831 aa overlap. Contains possible N-terminal region signal peptide sequence 1 4 11 17 [Reporter]SCO2035 (6E5)_PCR/6283 [Gene]SCO2035/5725 NC_003888 SC4G6.04c, possible membrane protein, len: 275 aa; similar to TR:Q9S2S3 (EMBL:AL096884) Streptomyces coelicolor hypothetical protein SC4G6.36, 266 aa; fasta scores: opt: 519 Z-score: 588.1 E(): 4e-25; 34.470% identity in 264 aa overlap 1 4 11 16 [Reporter]SCO7286 (7A5)_PCR/6282 [Gene]SCO7286/5724 NC_003888 SC5H1.06c, hypothetical protein, len: 327 aa; weakly similar e.g. SW:GS69_BACSU (EMBL:Y14082), YhdN, Bacillus subtilis general stress protein 69 (331 aa), fasta scores; opt: 419 z-score: 477.8 E(): 2.7e-19, 29.3% identity in 328 aa overlap and TR:Q40648 (EMBL:U46758), kob1, Oryza sativa potassium channel beta subunit protein (326 aa) (27.9% identity in 341 aa overlap). Weakly similar to SC5H1.21c, probable ion channel subunit (319 aa) (29.3% identity in 338 aa overlap) 1 4 11 15 [Reporter]SCO5562 (8M1)_PCR/6281 [Gene]SCO5562/5723 NC_003888 SC7A1.06, thiL, thiamine monphosphate kinase, len: 322aa; similar to many eg. TR:P95118 (EMBL:Z83018) hypothetical protein from Mycobacterium tuberculosis (333 aa) fasta scores; opt: 959, z-score: 1227.0, E(): 0, (51.1% identity in 319 aa overlap) and THIL_SALTY thiamine monphosphate kinase from Salmonella typhimurium (325 aa) fasta scores; opt: 511, z-score: 350.3, E(): 3.2e-12, (35.9% identity in 329 aa overlap). Note: thiAB (arbitrarily named) previously mapped to this region of the chromosome. 1 4 11 14 [Reporter]SCO2638 (9I1)_PCR/6280 [Gene]SCO2638/5722 NC_003888 SC8E4A.08c, putative membrane protein, len: 249 aa. Contains possible hydrophobic membrane spanning regions 4 2 18 5 [Reporter]SCO5153 (18D3)_PCR/3906 [Gene]SCO5153/3549 NC_003888 SCP8.16c, possible integral membrane protein, len: 198 aa; similar to TR:O86314 (EMBL:Z98260) Mycobacterium tuberculosis hypothetical 20.4 kD protein MTV006.03c, 180 aa; fasta scores: opt: 517 z-score: 619.2 E(): 5.1e-27; 52.9% identity in 157 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 11 13 [Reporter]SCO6478 (10E1)_PCR/6279 [Gene]SCO6478/5721 NC_003888 SC9C7.14c, conserved hypothetical protein, len: 132aa; similar to many hypotheticals eg. TR:P75896 (EMBL:AE000202) from Escherichia coli (128 aa) fasta scores; opt: 249, z-score: 350.3, E(): 3.2e-12, (39.2% identity in 125 aa overlap). Contains Pfam match to entry PF01042 DUF10, Domain of unknown function. 4 2 18 3 [Reporter]SCP1.307c (19L23)_PCR/3904 [Gene]SCP1.307c/3548 NC_003888 SCP1.307c, unknown, len: 136aa; similar to TR:Q9KY36 (EMBL:AL356892) hypothetical protein from Streptomyces coelicolor (111 aa) fasta scores; opt: 212, z-score: 263.6, E(): 3.3e-07, 37.0% identity in 108 aa overlap. 1 4 11 12 [Reporter]SCO2702 (11A1)_PCR/6278 [Gene]SCO2702/5720 NC_003888 SCC61A.23c, possible secreted protein, len: 171aa; contains a possible cleavable N-terminal signal sequence. 4 2 18 2 [Reporter]SCO7472 (20D23)_PCR/3903 [Gene]SCO7472/3547 NC_003888 SCBAC17A6.05, possible phenylacetic acid degradation protein PaaB, len: 103 aa: similar to many e.g. SW:P76078 (PAAB_ECOLI) phenylacetic acid degradation protein PaaB from Escherichia coli (95 aa) fasta scores; opt: 427, Z-score: 565.4, 66.667% identity (66.667% ungapped) in 90 aa overlap. 4 2 17 22 [Reporter]SCO1122 (1D19)_PCR/3901 [Gene]SCO1122/3546 NC_003888 2SCG38.15c, possible regulatory protein, len: 278 aa; similar to TR:Q9RIT6 (EMBL:AL133278) Streptomyces coelicolor putative lysR-family transcriptional regulator SCM11.26, 303 aa; fasta scores: opt: 243 z-score: 243.1 E(): 4.2e-06; 33.9% identity in 168 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 2 17 21 [Reporter]SCO5285 (2P15)_PCR/3900 [Gene]SCO5285/3545 NC_003888 SCCB12.09, lon, ATP-dependent protease, len: 807 aa; similar to SW:LON_MYCSM (EMBL:AF030688) Mycobacterium smegmatis ATP-dependent protease LA (EC 3.4.21.53) Lon, 779 aa; fasta scores: opt: 2550 z-score: 2489.6 E(): 0; 64.7% identity in 785 aa overlap. Contains Pfam matches to entries PF02190 LON, ATP-dependent protease La (LON) domain and PF00004 AAA, ATPases associated with various cellular activities (AAA) and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS01046 ATP-dependent serine proteases, lon family, serine active site 4 2 17 20 [Reporter]SCO6815 (3L15)_PCR/3899 [Gene]SCO6815/3544 NC_003888 SC1A2.24c, bi-domain ABC transporter permease protein, len: 603 aa. The N-terminus and C-terminus appear to be discrete domains, both of which are similar to many ABC transporter permease proteins e.g. The N-terminus is similar to Methanobacterium thermoautotrophicum TR:O27762 (EMBL:AE000929) phosphate transporter permease (291 aa), fasta scores opt: 467 z-score: 511.4 E(): 4.5e-21 32.5% identity in 268 aa overlap. The C-terminal is similar to Escherichia coli SW:PSTA_ECOLI (EMBL:X02723) phosphate transport system permease protein, PstA (296 aa), fasta scores opt: 396 z-score: 434.6 E(): 8.4e-17 30.1% identity in 286 aa overlap. Contains 2x Prosite hits to PS00402 Binding-protein-dependent transport systems inner membrane comp sign and 2x Pfam matches to entries PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Contains multiple possible membrane spanning hydrophobic domains. 4 2 17 19 [Reporter]SCO4830 (4H15)_PCR/3898 [Gene]SCO4830/3543 NC_003888 SC2A6.15, probable glycine betaine ABC transport system ATP-binding protein, len: 394 aa; similar to SW:OPAA_BACSU (EMBL:U17292) Bacillus subtilis glycine betaine transport ATP-binding protein OpuAa, 418 aa; fasta scores: opt: 1015 z-score: 1043.6 E(): 0; 48.5% identity in 338 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 1 23 6 [Reporter]SCO0893 (17K8)_PCR/1496 [Gene]SCO0893/1369 NC_003888 SCM1.26, possible membrane protein, len: 407 aa. Weakly similar to a hypothetical protein from Synechocystis sp. (strain PCC 6803) TR:P72959(EMBL:D90902) (343 aa), fasta scores opt: 215 z-score: 248.3 E(): 1.8e-06 28.7% identity in 352 aa overlap. Contains possible membrane spanning hydrophobic domains. 4 2 17 18 [Reporter]SCO2386 (5D15)_PCR/3897 [Gene]SCO2386/3542 NC_003888 SC4A7.14, hypothetical protein, len: 401 aa; similar to various hypothetical proteins, e.g. SW:YM42_MYCTU (EMBL:Z70692) Mycobacterium tuberculosis hypothetical 44.6 kD protein RV2242, 414 aa; fasta scores: opt: 581 z-score: 650.9 E(): 7.2e-29; 43.0% identity in 409 aa overlap 3 1 23 5 [Reporter]SCO0647 (18G8)_PCR/1495 [Gene]SCO0647/1368 NC_003888 SCF91.07, probable short chain oxidoreductase, len: 265 aa. Highly similar to many e.g. Klebsiella aerogenes SW:RIDH_KLEAE (EMBL:M25606) ribitol 2-dehydrogenase (EC 1.1.1.56) (249 aa), fasta scores opt: 311 z-score: 345.7 E(): 7e-12 31.7% identity in 230 aa overlap and Mycobacterium avium TR:AAD20367 (EMBL:AF125999) dehydrogenase (259 aa), fasta scores opt: 897 z-score: 975.2 E(): 0 56.8% identity in 257 aa overlap. Contains a Pfam match to entry PF00106 adh_short, short chain dehydrogenase and a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature. 4 2 17 17 [Reporter]SCO6537 (6P11)_PCR/3896 [Gene]SCO6537/3541 NC_003888 SC5C7.22c, unknown, len: 287 aa; similar to S. coelicolor hypothetical protein SC4G2.03 (E(): 2.2e-08, 29.2% identity in 257 aa overlap). Contains possible helix-turn-helix motif at aa 29-50 (Score 1251, +3.45 SD) 3 1 23 4 [Reporter]SCO6282 (19C8)_PCR/1494 [Gene]SCO6282/1367 NC_003888 SC1G7.08c, probable 3-oxoacyl-[acyl-carrier protein] reductase, len: 265 aa; similar to SW:FABG_BACSU (EMBL:U59433) Bacillus subtilis 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) FabG, 246 aa; fasta scores: opt: 531 Z-score: 586.7 bits: 116.1 E(): 4.3e-25; 41.036% identity in 251 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 4 2 17 16 [Reporter]SCO5661 (7L11)_PCR/3895 [Gene]SCO5661/3540 NC_003888 SC6A9.06, unknown, len: 241 aa; similar to hypothetical proteins e.g. Bacillus subtilis TR:O07592 (EMBL:Y14082) YhdW (243 aa), fasta SCORES; opt: 144 z-score: 267.8 E(): 1.1e-07, 25.0% identity in 236 aa overlap 3 1 23 3 [Reporter]SCO7519 (20O4)_PCR/1493 [Gene]SCO7519/1366 NC_003888 SCBAC25F8.11c, probable sugar acetyltransferase, len: 193 aa; similar to SW:MAA_ECOLI (EMBL:AJ223173) Escherichia coli maltose O-acetyl transferase (EC 2.3.1.79) Maa or B0459, 182 aa; fasta scores: opt: 534 Z-score: 607.5 bits: 119.1 E(): 3.2e-26; 43.407% identity in 182 aa overlap. Contains 3x Pfam matches to entry PF00132 hexapep, Bacterial transferase hexapeptide (four repeats) and match to Prosite entry PS00101 Hexapeptide-repeat containing-transferases signature 3 1 22 22 [Reporter]SCO1102 (1G24)_PCR/1491 [Gene]SCO1102/1365 NC_003888 2SCG4.18, possible integral membrane protein, len: 223 aa; similar to TR:Q06074 (EMBL:Z21972) Bacillus megaterium hypothetical 24.9 kD protein in cytochrome P450MEG gene 3' region, 216 aa; fasta scores: opt: 298 z-score: 353.5 E(): 3.3e-12; 27.5% identity in 207 aa overlap. Contains Pfam match to entry PF01569 PAP2, PAP2 superfamily. Also contains possible hydrophobic membrane spanning regions 3 1 22 21 [Reporter]SCO2950 (2C24)_PCR/1490 [Gene]SCO2950/1364 NC_003888 SCE59.09, hup, DNA-binding protein Hu (hs1), len: 93 aa; identical to SW:DBH_STRLI (EMBL:AB001381) Streptomyces lividans DNA-binding protein hu (HS1) Hup, 93 aa and highly similar to SW:DBH_BACSU (EMBL:X66448) Bacillus subtilis DNA-binding protein II (HB) (HU), 92 aa; fasta scores: opt: 237 z-score: 311.2 E(): 6.4e-10; 40.9% identity in 93 aa overlap. Contains Pfam match to entry PF00216 Bac_DNA_binding, Bacterial DNA-binding protein 3 1 22 20 [Reporter]SCO1388 (3O20)_PCR/1489 [Gene]SCO1388/1363 NC_003888 SC1A8A.08c, possible mannose-1-phosphate guanyltransferase, len: 831aa; similar to many eg. TR:P74589 (EMBL:D90916) putative mannose-1-phosphate guanyltransferase from Synechocystis sp. (strain PCC 6803) (843 aa) fasta scores; opt: 2184, z-score: 2364.6, E(): 0, 39.7% identity in 817 aa overlap and SW:P41940 (MPG1_YEAST) mannose-1-phosphate guanyltransferase from Saccharomyces cerevisiae (Baker's yeast) (361 aa). Contains Pfam match to entry PF00483 NTP_transferase, Nucleotidyl transferase, four Pfam match to entry PF00132 hexapep, Bacterial transferase hexapeptide (four repeats) and Pfam match to entry PF00408 PGM_PMM, Phosphoglucomutase/phosphomannomutase. 3 1 22 19 [Reporter]SCO7627 (4K20)_PCR/1488 [Gene]SCO7627/1362 NC_003888 SC2H2.25, conserved hypothetical protein, len: 193 aa; similar to TR:Q9XBC6 (EMBL:AL078635) Amycolatopsis orientalis hypothetical 22.9 kDa protein CZA382.25, 205 aa; fasta scores: opt: 202 z-score: 256.0 E(): 1e-06; 31.7% identity in 189 aa overlap 3 1 22 18 [Reporter]SCO7113 (5G20)_PCR/1487 [Gene]SCO7113/1361 NC_003888 SC4B10.14, possible integral membrane protein, len: 211 aa; similar to TR:Q9RCU9 (EMBL:AL136149) Streptomyces coelicolor putative integral membrane protein SCM2.15c, 219 aa; fasta scores: opt: 239 z-score: 284.5 E(): 2.2e-08; 34.5% identity in 220 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 22 17 [Reporter]SCO7569 (6C20)_PCR/1486 [Gene]SCO7569/1360 NC_003888 SC5F1.23, conserved hypothetical protein, len: 263 aa; similar to TR:P74740 (EMBL:D90917) Synechocystis sp. hypothetical 30.2 kDa protein SLR0596, 273 aa; fasta scores: opt: 248 z-score: 279.7 E(): 4.7e-08; 31.6% identity in 266 aa overlap 1 4 11 11 [Reporter]SCO4671 (11M21)_PCR/6277 [Gene]SCO4671/5719 NC_003888 SCD40A.17c, probable lysR-family regulatory protein, len: 315 aa; similar to SW:MPRR_STRCO (EMBL:Z11929) Streptomyces coelicolor small neutral protease regulatory protein MprR, 316 aa; fasta scores: opt: 541 z-score: 619.1 E(): 4.7e-27; 36.7% identity in 308 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Contains also possible helix-turn-helix motif at residues 18..39 (+4.31 SD). Contains also TTA leucine codon, possible target for bldA regulation 1 4 11 10 [Reporter]SCO3526 (12I21)_PCR/6276 [Gene]SCO3526/5718 NC_003888 SCE2.07, hypothetical protein, len: 427 aa; C-terminal region similar to TR:Q9L1W2 (EMBL:AL138667) Streptomyces coelicolor hypothetical protein (fragment) SC3D9.17c, 235 aa; fasta scores: opt: 1120 Z-score: 1250.9 E(): 4.8e-62; 71.795% identity in 234 aa overlap 1 4 11 9 [Reporter]SCO5023 (13E21)_PCR/6275 [Gene]SCO5023/5717 NC_003888 SCK15.25, possible secreted protein, len: 336 aa; similar to TR:CAB77312 (EMBL:AL160312) Streptomyces coelicolor putative secreted protein SCD35.28c, 361 aa; fasta scores: opt: 890 z-score: 972.2 E(): 0; 47.3% identity in 357 aa overlap. Contains possible N-terminal region signal peptide sequence 1 4 11 8 [Reporter]SCO0169 (14A21)_PCR/6274 [Gene]SCO0169/5716 NC_003888 SCJ1.18, conserved hypothetical protein, len: 217 aa. Duplication of DNA region complement(17965..18576). Similar to many hypothetical proteins including: Thermotoga maritima TR:AAD36216 (EMBL; AE001771) conserved hypothetical protein (215 aa), fasta scores opt: 239 z-score: 287.3 E(): 1.2e-08 29.1% identity in 175 aa overlap and S. coelicolor SCJ1.19c (223 aa), fasta scores opt: 711 z-score: 702.0 E(): 7.1e-34 56.7% identity in 215 aa overlap.Contains 2xPfam match to entry PF00571 CBS, CBS domains. 1 4 11 7 [Reporter]SCO0183 (15M17)_PCR/6273 [Gene]SCO0183/5715 NC_003888 SCJ1.32, unknown,len: 415 aa. Likely to have arisen as a result of a duplication of SCF43A.31/32, followed by a deletion event generating a possible CDS with the 5' coding sequence of SCF43A.31 and the 3' coding sequence of SCF43A.32. Similarity scores are as follows: to Streptomyces coelicolor TR:CAB48918 (EMBL; AL096837) putative oxidoreductase SCF43A.31 (520 aa), fasta scores opt: 1191 z-score: 1365.3 E(): 0 64.2% identity in 307 aa overlap and to Streptomyces coelicolor TR:CAB48919(EMBL; AL096837) putative deoxyribodipyrimidine photolyase SCF43A.32 (458 aa), opt: 909 z-score: 1043.2 E(): 0 45.7% identity in 418 aa overlap. Contains a PS00394 DNA photolyases class 1 signature 1 and a PS00691 DNA photolyases class 1 signature 2. 1 4 11 6 [Reporter]SCO1276 (16I17)_PCR/6272 [Gene]SCO1276/5714 NC_003888 2SCG18.23, sigJ, RNA polymerase ECF sigma factor, len: 198 aa; identical to previously sequenced TR:AAF35371 (EMBL:AF225460) Streptomyces coelicolor RNA polymerase ECF sigma factor SigJ, 198 aa. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 1 4 11 5 [Reporter]SCO0152 (17E17)_PCR/6271 [Gene]SCO0152/5713 NC_003888 SCJ1.01, possible transmembrane transport protein, partial CDS, len: >284 aa; similar to S. coelicolor putative transmembrane transport protein SC5B8.17 TR:O70003 (EMBL: AL022374) (530 aa), fasta scores; opt: 513 z-score: 549.9 E(): 2.9e-23 38.1% identity in 270 aa overlap, Rhodobacter capsulatus transmembrane protein TR:O68042(EMBL: AF010496)(578 aa), fasta scores opt: 243 z-score: 263.6 E(): 2.5e-07 26.3% identity in 281 aa overlap and Pseudomonas putida TR:O50471 (EMBL; Z71176) phenylacetic acid permease (520 aa), fasta scores opt: 233 z-score: 253.6 E(): 9.1e-07 31.1% identity in 293 aa overlap. Contains probable membrane spanning hydrophobic domains.,SCJ33.16, possible transmembrane transport protein, partial CDS, len: >288 aa. Similar to several including: Pseudomonas putida TR:O50471 (EMBL; Z71176) phenylacetic acid permease (520 aa), fasta scores opt: 337 z-score: 380.9 E(): 7.5e-14 29.4% identity in 269 aa overlap and Streptomyces coelicolor TR:O70003 (EMBL; AL022374) putative transmembrane transport SC5B8.17 (530 aa), fasta scores opt: 462 z-score: 520.1 E(): 1.3e-21 31.9% identity in 279 aa overlap. Overlaps with and extends into CDS SCJ1.01. Contains possible membrane spanning hydrophobic domains. 1 4 11 3 [Reporter]SCP1.121 (19M13)_PCR/6269 [Gene]SCP1.121/5712 NC_003888 SCP1.121, unknown, len: 178aa; Contains a TTA encoded leucine at residue 76, possible target for bldA regulation. 4 2 17 15 [Reporter]SCO5296 (8H11)_PCR/3894 [Gene]SCO5296/3539 NC_003888 SC6G9.37, possible tetR-family transcriptional regulator, len: 217 aa; similar to many hypothetical proteins, especially from mycobacteria e.g. TR:Q49662 (EMBL:U00012) Mycobacterium leprae hypothetical protein (242 aa), fasta scores; opt: 216 z-score: 270.8 E(): 9.4e-08, 28.8% identity in 222 aa overlap. Weak similarity to some putative transcriptional regulators. Contains probable helix-turn-helix motif at aa 39-60 (Score 1937, +5.78 SD). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 1 4 11 2 [Reporter]SCO7701 (20E13)_PCR/6268 [Gene]SCO7701/5711 NC_003888 SCBAC12C8.02, possible methyltransferase, len: 396aa: similar to many eg. TR:Q9F1V7 (EMBL:AB040071) methyltransferase from Streptomyces griseus (316 aa) fasta scores; opt: 1550, Z-score: 1809.5, 82.246% identity (82.545% ungapped) in 276 aa overlap. Previously sequenced as TR:Q9F1Y5 (EMBL:AB035202) ORF4. 4 2 17 14 [Reporter]SCO5516 (9D11)_PCR/3893 [Gene]SCO5516/3538 NC_003888 SC8D9.28c, probable integral membrane efflux protein, len: 521aa; Similar to many efflux proteins (confirmed and proposed) most of which appear to be dedicated to drug resistance/export egs. TR:O33968 (EMBL:U80063) putative transmembrane, proton-dependent transport protein from Streptomyces cinnamomeus (519 aa) fasta scores; opt: 1342, z-score: 1386.9, E(): 0, (46.5% identity in 503 aa overlap) and TR:O52557 (EMBL:AF040570) rifamycin efflux protein from Amycolatopsis mediterranei (522 aa) fasta scores; opt: 988, z-score: 1022.7, E(): 0, (38.0% identity in 523 aa overlap). Contains multiple potential membrane spanning regions. 1 4 10 22 [Reporter]SCO5266 (1E9)_PCR/6266 [Gene]SCO5266/5710 NC_003888 2SC7G11.28, putative membrane protei, len: 277 aa; similar to N-terminal region of TR:CAB76994 (EMBL:AL159178) Streptomyces coelicolor putative integral membrane protein SCH22.25, 456 aa; fasta scores: opt: 141 z-score: 157.1 E(): 0.28; 29.3% identity in 263 aa overlap. Contains matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Contains possible hydrophobic membrane spanning region 4 2 17 13 [Reporter]SCO2513 (10P7)_PCR/3892 [Gene]SCO2513/3537 NC_003888 SCC121.16, hypothetical protein, len: 277 aa; similar to various hypothetical proteins, e.g. TR:Q9X8M2 (EMBL:AL049628) Streptomyces coelicolor hypothetical 31.5 kD protein SCE94.16c, 280 aa; fasta scores: opt: 538 z-score: 655.5 E(): 4.1e-29; 37.1% identity in 259 aa overlap 4 2 17 12 [Reporter]SCO4388 (11L7)_PCR/3891 [Gene]SCO4388/3536 NC_003888 SCD10.20, probable citrate synthase, len: 387 aa; similar to SW:CISY_BACSU (EMBL:U05256) Bacillus subtilis citrate synthase I (EC 4.1.3.7) CitA, 366 aa; fasta scores: opt: 615 z-score: 685.9 E(): 9.9e-31; 36.7% identity in 360 aa overlap and to TR:O70008 (EMBL:AL022374) Streptomyces coelicolor citrate synthase (EC 4.1.3.7) SC5B8.22, 390 aa; fasta scores: opt: 780 z-score: 867.9 E(): 0; 41.0% identity in 371 aa overlap. Contains 2 Pfam matches to entry PF00285 citrate_synt, Citrate synthase and match to Prosite entry PS00480 Citrate synthase signature 4 2 17 11 [Reporter]SCO4462 (12H7)_PCR/3890 [Gene]SCO4462/3535 NC_003888 SCD65.05, possible integral membrane protein, len: 415 aa; similar to TR:P75810 (EMBL:AE000186) Escherichia coli MosC protein, 402 aa; fasta scores: opt: 1317 z-score: 1364.7 E(): 0; 54.1% identity in 392 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 17 9 [Reporter]SCO0164 (14P3)_PCR/3888 [Gene]SCO0164/3534 NC_003888 SCJ1.13, putative cation-transporting ATPase, len: 776 aa, similar to many including: Mycobacterium tuberculosis SW:CTPG_MYCTU (EMBL:Z74025) probable cation-transporting ATPase G (771 aa), fasta scores opt: 1050 z-score: 1095.0 E(): 0 37.6% identity in 588 aa overlap, Mycobacterium tuberculosis TR:O66027 (EMBL:U82820)(EMBL metal-transporting ATPase MTA72 (680 aa), fasta scores opt: 1036 z-score: 1081.2 E(): 0 33.8% identity in 577 aa overlap and Helicobacter felis SW:COPA_HELFE (EMBL: AJ001932) copper-transporting ATPase (EC 3.6.1.36) (732 aa), fasta scores opt: 904 z-score: 943.3 E(): 0, 33.1% identity in 547 aa overlap.Contains possible membrane spanning hydrophobic domains, a Pfam match to entry PF00122 E1-E2_ATPase, E1-E2 ATPases and a PS00154 E1-E2 ATPases phosphorylation site. 4 2 17 8 [Reporter]SCO1805 (15L3)_PCR/3887 [Gene]SCO1805/3533 NC_003888 SCI33.04, possible membrane protein, len: 244 aa; C-terminal region highly similar to TR:Q9XAS4 (EMBL:AL078618) Streptomyces coelicolor hypothetical 17.2 kDa protein SC10A7.01, 163 aa; fasta scores: opt: 620 z-score: 633.5 E(): 9.9e-28; 63.1% identity in 157 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 22 16 [Reporter]SCO2116 (7O16)_PCR/1485 [Gene]SCO2116/1359 NC_003888 SC6E10.10, possible secreted protein, len: 943 aa; unknown function, similar in parts to bacterial AmpD (amidase) proteins e.g. TR:Q46959 (EMBL:U40805), AmpD, Enterobacter cloacae anhydro-N-acetylmuramyl-tripeptide amidase (187 aa), fasta scores; opt: 208 z-score: 224.7 E(): 3.4e-05, 37.4% identity in 123 aa overlap. Similar to parts of others from S.coelicolor e.g. SCH35.49C (EMBL:AL078610) S.coelicolor hypothetical protein (410 aa) (37.7% identity in 252 aa overlap). Contains possible N-terminal signal sequence. The C-terminus contains 5x degenerate 50 aa repeats 4 2 17 7 [Reporter]SCO0632 (16H3)_PCR/3886 [Gene]SCO0632/3532 NC_003888 SCF56.16, probable RNA polymerase sigma factor, len: 194 aa; similar to TR:CAB50938 (EMBL:AL096849) Streptomyces coelicolor SCI11.12c, 185 aa; fasta scores: opt: 710 z-score: 848.0 E(): 0; 62.4% identity in 173 aa overlap and to SW:SIGW_BACSU (EMBL:AB002150) Bacillus subtilis RNA polymerase sigma factor SigW, 187 aa; fasta scores: opt: 209 z-score: 258.8 E(): 4.9e-07; 24.9% identity in 181 aa overlap. Contains a possible helix-turn-helix motif at residues 159..180 (+2.77 SD) 3 1 22 15 [Reporter]SCO2008 (8K16)_PCR/1484 [Gene]SCO2008/1358 NC_003888 SC7H2.22, possible branched chain amino acid binding protein, len: 481aa; similar to many eg. SW:LIVK_ECOLI leucine-specific binding protein from Escherichia coli (369 aa) fasta scores; opt: 360, z-score: 374.0, E(): 1.6e-13, (28.4% identity in 352 aa overlap). Contains possible N-terminal signal sequence. 4 2 17 6 [Reporter]SCO0427 (17D3)_PCR/3885 [Gene]SCO0427/3531 NC_003888 SCF51A.05, possible hydrolase, len: 278 aa. Similar to many e.g. Pseudomonas fluorescens SW:PRXC_PSEFL (EMBL:AF031153) non-heme chloroperoxidase (EC 1.11.1.10) (chloride peroxidase), fasta scores opt: 243 z-score: 282.1 E(): 2.4e-08 28.4% identity in 275 aa overlap and many putative hydrolases: Streptomyces coelicolor TR:CAB44393 (EMBL; AL078610) putative hydrolase SCH35.34C (269 aa), fasta scores opt: 259 z-score: 300.1 E(): 2.3e-09 29.7% identity in 269 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 3 1 22 14 [Reporter]SCO7545 (9G16)_PCR/1483 [Gene]SCO7545/1357 NC_003888 SC8G12.21, possible ABC-transport protein, ATP-binding component, len: 270 aa. Similar to multiple ABC-transport proteins e.g. Escherichia coli SW:TAUB_ECOLI(EMBL:D85613) taurine transport ATP-binding protein, TauB (255 aa), fasta scores opt: 665 z-score: 772.8 E(): 0 43.4% identity in 235 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop) and a Pfam match to entry PF00005 ABC_tran, ABC transporter. 4 2 17 5 [Reporter]SCO1762 (17P23)_PCR/3884 [Gene]SCO1762/3530 NC_003888 SCI51.02c, possible chorismate mutase, len: 120 aa; similar to e.g. SW:CHMU_BACSU (EMBL:M32278), AroH, Bacillus subtilis chorismate mutase (127 aa), fasta scores; opt: 307 z-score: 407.7 E(): 2.2e-15, 38.1% identity in 118 aa overlap 3 1 22 13 [Reporter]SCO2440 (10C16)_PCR/1482 [Gene]SCO2440/1356 NC_003888 SCC24.11c, probable transcriptional regulator, len: 257 aa; similar to SW:KDGR_ECOLI (EMBL:AE000277) Escherichia coli transcriptional regulator KdgR, 263 aa; fasta scores: opt: 446 z-score: 510.4 E(): 5.4e-21; 32.1% identity in 249 aa overlap. Contains Pfam match to entry PF01614 IclR, Bacterial transcriptional regulator 3 1 22 12 [Reporter]SCO4558 (11O12)_PCR/1481 [Gene]SCO4558/1355 NC_003888 SCD16A.25, possible acetyltransferase, len: 187 aa; similar to SW:YKKB_BACSU hypothetical protein from Bacillus subtilis (172 aa) fasta scores; opt: 237, z-score: 294.8, E(): 4.3e-09, (30.8% identity in 146 aa overlap). Also similar to the N-terminal half of TR:Q53796 (EMBL:L26955) bleomycin acetyltransferase from the bleomycin-resistance region of Streptomyces verticillus (301 aa) fasta scores; opt: 163, z-score: 203.0, E(): 0.00056, (33.5% identity in 155 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 3 1 22 11 [Reporter]SCO4266 (12K12)_PCR/1480 [Gene]SCO4266/1354 NC_003888 SCD86A.03c, probable oxidoreductase, len: 340 aa; similar to TR:Q53927 (EMBL:X62373) Streptomyces coelicolor genes for hydroxyacyl-CoA dehydrogenase, 329 aa; fasta scores: opt: 635 z-score: 652.9 E(): 6.7e-29; 37.9% identity in 335 aa overlap and to SW:QOR_ECOLI (EMBL:L02312) Escherichia coli quinone oxidoreductase (EC 1.6.5.5) Qor, 327 aa; fasta scores: opt: 395 z-score: 410.1 E(): 2.2e-15; 30.7% identity in 339 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases 3 1 22 10 [Reporter]SCO3016 (13G12)_PCR/1479 [Gene]SCO3016/1353 NC_003888 SCE33.18, possible integral membrane protein, len: 231 aa; similar to TR:O69658 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical 23.8 kD protein MTV025.038, 217 aa; fasta scores: opt: 419 z-score: 493.8 E(): 4.8e-20; 36.0% identity in 211 aa overlap. Contains possible hydrophobic membrane spanning region 3 1 22 9 [Reporter]SCO1161 (14C12)_PCR/1478 [Gene]SCO1161/1352 NC_003888 SCG8A.15c, probable integral membrane protein, len: 244aa; N-terminal region similar to that of TR:CAB76246 (EMBL:X82447) CoxC protein from Pseudomonas carboxydovorans (402 aa) fasta scores; opt: 387, z-score: 455.6, E(): 6.2e-18, 34.6% identity in 243 aa overlap. Contains possible membrane-spanning hydrophobic regions. 3 1 22 8 [Reporter]SCO0458 (15O8)_PCR/1477 [Gene]SCO0458/1351 NC_003888 SCF51A.36, probable Beta-glucosidase (EC 3.2.1.21), len: 762 aa. Highly similar to many e.g. Escherichia coli SW:BGLX_ECOLI (EMBL; U15049) periplasmic Beta-glucosidase precursor (EC 3.2.1.21) (765 aa), fasta scores opt: 851 z-score: 896.9 E(): 0 32.2% identity in 773 aa overlap. Contains 2x Pfam matches to entry PF00933 Glyco_hydro_3, Glycosyl hydrolase family 3. 3 1 22 7 [Reporter]SCO0430 (16K8)_PCR/1476 [Gene]SCO0430/1350 NC_003888 SCF51A.08, possible tetR family transcriptional regulator, len: 227 aa. Similar to several putative regulators e.g. Streptomyces coelicolor TR:CAB46405 (EMBL; AL096743) putative transcriptional regulator SCI7.22c (202 aa), fasta scores opt: 428 z-score: 495.6 E(): 3e-20 41.1% identity in 202 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a possible helix-turn-helix motif between residues 71..92 (+3.86 SD). 1 4 10 21 [Reporter]SCO5852 (2A9)_PCR/6265 [Gene]SCO5852/5709 NC_003888 SC9B10.19, agaY, possible tagatose-bisphosphate aldolase, len: 282 aa; similar to eg. AGAY_ECOLI P42908 tagatose-bisphosphate aldolase agaY (286 aa), fasta scores; opt: 585 z-score: 725.0 E(): 3.2e-33, 37.6% identity in 279 aa overlap 1 4 10 20 [Reporter]SCO4720 (3M5)_PCR/6264 [Gene]SCO4720/5708 NC_003888 SCD31.45, rpmD, 50S ribosomal protein L30, len: 60 aa; identical to previously sequenced SW:RL30_STRCO (EMBL:X83011) Streptomyces coelicolor 50S ribosomal protein L30 RpmD, 60 aa. Contains Pfam match to entry PF00327 Ribosomal_L30, Ribosomal protein L30p/L7e and match to Prosite entry PS00634 Ribosomal protein L30 signature 1 4 10 19 [Reporter]SCO6614 (4I5)_PCR/6263 [Gene]SCO6614/5707 NC_003888 SC1F2.11, probable membrane protein, len: 369 aa; contains a possible transmembrane domain around aa 100 1 4 10 18 [Reporter]SCO5715 (5E5)_PCR/6262 [Gene]SCO5715/5706 NC_003888 SC3C3.01c, probable peptide transport system integral membrane protein, partial CDS, len: >229 aa; similar to many e.g. DPPB_ECOLI dipeptide transport system permease protein (339 aa), fasta scores; opt: 359 z-score: 654.6 E(): 3.3e-29, 35.3% identity in 238 aa overlap. Also similar to S. coelicolor SC2A11.12 (E(): 5.6e-12, 30.7% identity in 244 aa overlap),SC9F2.01, partial CDS, possible peptide transport system permease, len:>140aa; constitutes the C-terminal half of a CDS whose N-terminal region (TR:O86635) lies on Streptomyces coelicolor cosmid SC3C3 (EMBL:AL031231). Similar to many dipeptide transport system permeases (confirmed and proposed) eg. SW:DPPB_ECOLI dipeptide transport system permease, DppB, from Escherichia coli (339 aa) fasta scores; opt: 391, z-score: 499.6, E(): 1.6e-20, (43.8% identity in 137 aa overlap). Contains probable membrane spanning hydrophobic regions. Also contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 24.30, E-value 0.0029. 1 4 10 17 [Reporter]SCO6650 (6A5)_PCR/6261 [Gene]SCO6650/5705 NC_003888 SC4G2.24, unknown, len: 132 aa 1 4 10 16 [Reporter]SCO3598 (7M1)_PCR/6260 [Gene]SCO3598/5704 NC_003888 SC66T3.09c, possible membrane protein, len: 254 aa; unknown function, similar to TR:O26720 (EMBL:AE000843) Methanobacterium thermoautotrophicum hypothetical protein, fasta scores; opt: 319 z-score: 383.6 E(): 4.9e-14, 28.7% identity in 251 aa overlap. Contains hydrophobic, possible membrane-spanning regions 1 4 10 15 [Reporter]SCO5558 (8I1)_PCR/6259 [Gene]SCO5558/5703 NC_003888 SC7A1.02, possible acyltransferase, len: 264aa; similar to several proposed acyltransferases eg. TR:O07809 (EMBL:Z97188) acyltransferase from Mycobacterium tuberculosis (251 aa) fasta scores; opt: 266, z-score: 384.5, E(): 4e-14, (29.7% identity in 229 aa overlap). 1 4 10 14 [Reporter]SCO6599 (9E1)_PCR/6258 [Gene]SCO6599/5702 NC_003888 SC8A6.20c, probable transcriptional regulator, len: 217 aa; some similarity to members of the tetR family e.g. TER4_ECOLI tetracycline repressor protein class D (217 aa), fasta scores; opt: 158 z-score: 233.4 E(): 9.2e-06, 35.2% identity in 88 aa overlap. Contains probable helix-turn-helix motif at aa 29-50 (Score 1279, +3.54 SD) and Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family, score 31.90, E-value 4.2e-07 4 2 17 4 [Reporter]SCP1.172 (18L23)_PCR/3883 [Gene]SCP1.172/3529 NC_003888 SCP1.172, possible integral membrane protein, len: 63aa; weak similarity to TR:Q9L0R5 (EMBL:AL160312) putative integral membrane protein from Streptomyces coelicolor (117 aa) fasta scores; opt: 78, z-score: 131.4, E(): 7.5, 40.7% identity in 27 aa overlap. Contains possible membrane-spanning hydrophobic regions. 1 4 10 13 [Reporter]SCO6474 (10A1)_PCR/6257 [Gene]SCO6474/5701 NC_003888 SC9C7.10c, possible transcriptional regulator, len: 272 aa; similar to many hypotheticals and SW:TCMR_STRGA TcmR, tetracenomycin C transcriptional repressor from Streptomyces glaucescens (226 aa) fasta scores; opt: 221, z-score: 260.3, E(): 3.3e-07, (31.7% identity in 221 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Also contains a probable helix-turn-helix from residue 53 to 74 (+3.47 SD). 4 2 17 3 [Reporter]SCO4978 (19H23)_PCR/3882 [Gene]SCO4978/3528 NC_003888 2SCK36.01c, conserved hypothetical membrane protein, len: 234 aa; similar to SW:YA85_MYCTU (EMBL:AL021897) Mycobacterium tuberculosis hypothetical 26.0 kDa protein RV1085c or MTV017.38c, 242 aa; fasta scores: opt: 563 Z-score: 653.6 bits: 128.3 E(): 8.6e-29; 39.524% identity in 210 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 10 12 [Reporter]SCO2754 (10M21)_PCR/6256 [Gene]SCO2754/5700 NC_003888 SCC57A.25, unknown, len: 268 aa. Weakly similar to Streptomyces coelicolor TR:Q9Z596(EMBL:AL035478) putative oxidoreductase (279 aa), fasta scores opt: 185 z-score: 212.6 E(): 0.00019 29.6% identity in 247 aa overlap. 4 2 17 2 [Reporter]SCO5066 (20P19)_PCR/3881 [Gene]SCO5066/3527 NC_003888 SCBAC20F6.09, possible beta-lactamase, len: 304 aa; similar to TR:Q9X7X2 (EMBL:AL049485) Streptomyces coelicolor putative beta-lactamase SC6E5.26c, 310 aa; fasta scores: opt: 598 Z-score: 696.3 bits: 136.9 E(): 3.4e-31; 53.115% identity in 305 aa overlap and to SW:BLA1_XANMA (EMBL:X75074) Xanthomonas maltophilia metallo-beta-lactamase L1 precursor (beta-lactamase, type II) (EC 3.5.2.6), 290 aa; fasta scores: opt: 146 Z-score: 175.9 bits: 40.6 E(): 0.033; 25.952% identity in 289 aa overlap. Contains Pfam match to entry PF00753 lactamase_B, Metallo-beta-lactamase superfamily and match to Prosite entry PS00136 Serine proteases, subtilase family, aspartic acid active site 4 2 16 22 [Reporter]SCO1224 (1P15)_PCR/3879 [Gene]SCO1224/3526 NC_003888 2SCG58.24c, possible sugar-phosphate isomerase, len: 152 aa; similar to SW:RPIB_ECOLI (EMBL:X82203) Escherichia coli ribose 5-phosphate isomerase B (EC 5.3.1.6) RpiB, 149 aa; fasta scores: opt: 248 z-score: 314.7 E(): 4.6e-10; 36.6% identity in 134 aa overlap 4 2 16 21 [Reporter]SCO2571 (2L15)_PCR/3878 [Gene]SCO2571/3525 NC_003888 SCC123.09c, leuS, leucyl-tRNA synthetase (EC 6.1.1.4) len: 966 aa. Highly similar to many leucyl-tRNA synthetases including: Bacillus subtilis SW:SYL_BACSU(EMBL:M88581) (804 aa), fasta scores opt: 508 z-score: 575.2 E(): 1.2e-24 48.1% identity in 941 aa overlap and Mycobacterium leprae SW:SYL_MYCLE(EMBL:Y14967) (972 aa), fasta scores opt: 3887 z-score: 4430.5 E():0 60.1% identity in 977 aa overlap. Contains a Prosite hit to PS00178 Amino-acyl-transfer RNA synthetases class-I signature and a Pfam match to entry PF00133 tRNA-synt_1, tRNA synthetases class I (I, L, M and V). 4 2 16 20 [Reporter]SCO7796 (3H15)_PCR/3877 [Gene]SCO7796/3524 NC_003888 SC10B8A.06, unknown, len: 219 aa; located between inverted DNA repeat units. 4 2 16 19 [Reporter]SCO4822 (4D15)_PCR/3876 [Gene]SCO4822/3523 NC_003888 SC2A6.07c, possible integral membrane protein, len: 195 aa. Contains possible hydrophobic membrane spanning regions 3 1 22 6 [Reporter]SCO4866 (17G8)_PCR/1475 [Gene]SCO4866/1349 NC_003888 SCK20.07, possible ECF sigma factor, len: 220 aa; similar to TR:Q9RIT1 (EMBL:AJ010584) Streptomyces coelicolor ECF sigma factor, 264 aa; fasta scores: opt: 280 z-score: 329.1 E(): 8.5e-11; 31.9% identity in 182 aa overlap and to Streptomyces coelicolor putative ECF sigma factor SCK20.05, 225 aa; fasta scores: opt: 671 z-score: 614.8 E(): 1.4e-28; 59.5% identity in 185 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 4 2 16 18 [Reporter]SCO6365 (5P11)_PCR/3875 [Gene]SCO6365/3522 NC_003888 SC4A2.01c, probable integral membrane protein, partial CDS, len: > 653 aa; overlaps and extends SC3A7.33c,SC3A7.33c, unknown, partial CDS, len: >259 aa 3 1 22 5 [Reporter]SCO0611 (18C8)_PCR/1474 [Gene]SCO0611/1348 NC_003888 SCF55.35, putative membrane protein, len: 254 aa; similar to various hypothetical proteins, e.g. TR:O26968 (EMBL:AE000864) Methanobacterium thermoautotrophicum conserved protein MTH882, 261 aa; fasta scores; opt: 202 z-score: 221.3 E(): 5.9e-05; 27.4% identity in 252 aa overlap 4 2 16 17 [Reporter]SCO6529 (6L11)_PCR/3874 [Gene]SCO6529/3521 NC_003888 SC5C7.14, probable ATP/GTP binding protein, len: 353 aa; similar to TR:Q9RV59 (EMBL:AE001966) Deinococcus radiodurans conserved hypothetical protein DR1171, 340 aa; fasta scores: opt: 453 Z-score: 502.2 E(): 2.4e-20; 33.692% identity in 279 aa overlap. Contains Prosite match to entry PS00017 ATP/GTP-binding 3 1 22 4 [Reporter]SCO4907 (19O4)_PCR/1473 [Gene]SCO4907/1347 NC_003888 2SCK8.33c, afsQ1, transcriptional regulatory protein, len: 225 aa; identical to previously sequenced SW:AFQ1_STRCO (EMBL:D10654) Streptomyces coelicolor transcriptional regulatory protein AfsQ1, 225 aa and highly similar to TR:Q9K455 (EMBL:AL359215) Streptomyces coelicolor putative two-component system response regulator SC2H12.29, 229 aa; fasta scores: opt: 920 z-score: 981.8 E(): 0; 61.0% identity in 228 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 4 2 16 16 [Reporter]SCO5702 (7H11)_PCR/3873 [Gene]SCO5702/3520 NC_003888 SC5H4.26c, possible lipoprotein, len: 187 aa; similar to TR:O05816 (EMBL:Z95207) Mycobacterium tuberculosis hypothetical 17.7 kD protein MTCY24A1.14c, 181 aa; fasta scores: opt: 152 z-score: 161.9 E(): 0.15; 32.6% identity in 178 aa overlap. Contains possible N-terminal region signal peptide sequence and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site 3 1 22 3 [Reporter]SCO6096 (20K4)_PCR/1472 [Gene]SCO6096/1346 NC_003888 SCBAC1A6.20c, probable lipoprotein, len: 367 aa; similar to SW:SSUA_BACSU (EMBL:L16808) Bacillus subtilis putative aliphatic sulfonates binding protein precursor SsuA, 332 aa; fasta scores: opt: 280 z-score: 316.7 E(): 4.6e-10; 28.2% identity in 358 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 3 1 21 22 [Reporter]SCO1098 (1C24)_PCR/1469 [Gene]SCO1098/1345 NC_003888 2SCG4.14c, probable MarR-family regulatory protein, len: 191 aa; similar to SW:PECS_ERWCH (EMBL:X74409) Erwinia chrysanthemi regulatory protein PecS, 166 aa; fasta scores: opt: 302 z-score: 329.2 E(): 7.5e-11; 34.0% identity in 150 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 3 1 21 21 [Reporter]SCO6832 (2O20)_PCR/1468 [Gene]SCO6832/1344 NC_003888 SC4A9.09, mutA, methylmalonyl-CoA mutase (EC 5.4.99.2), len: 531 aa. Highly similar to many members of the methylmalonyll-CoA mutase family including: Sinorhizobium meliloti TR:O86028(EMBL:AF080073) methylmalonyll-CoA mutase (EC 5.4.99.2) (712 aa), fasta scores opt: 1720 z-score: 1946.7 E(): 0 52.2% identity in 515 aa overlap and Pyrococcus abyssi TR:CAB49756(EMBL:AJ248285) methylmalonyl-CoA mutase, subunit alpha, N-terminus (562 aa), fasta scores opt: 2180 z-score: 2469.0 E():0 62.7% identity in 518 aa overlap. Also highly similar to Streptomyces coelicolor TR:Q9X949(EMBL:AJ237976) isobutyryl-CoA mutase A (EC 5.4.99.13), SC8F4.19 (566 aa), fasta scores opt: 1515 z-score: 1556.8 E():0 50.5% identity in 541 aa overlap. Contains a Pfam match to entry PF01642 MM_CoA_mutase, Methylmalonyl-CoA mutase. 3 1 21 20 [Reporter]SCO1384 (3K20)_PCR/1467 [Gene]SCO1384/1343 NC_003888 SC1A8A.04c, hypothetical protein, len: 267aa; similar to but longer than SW:Q50606 (Y0DG_MYCTU) hypothetical protein from Mycobacterium tuberculosis (162 aa) fasta scores; opt: 503, z-score: 488.2, E(): 9.2e-20, 56.0% identity in 141 aa overlap. Contains Pfam match to entry PF00498 FHA, FHA domain 3 1 21 19 [Reporter]SCO5865 (4G20)_PCR/1466 [Gene]SCO5865/1342 NC_003888 SC2E9.06, unknown, len: 317 aa 3 1 21 18 [Reporter]SCO7108 (5C20)_PCR/1465 [Gene]SCO7108/1341 NC_003888 SC4B10.09c, conserved hypothetical protein, len: 248 aa; similar to TR:Q9S1U9 (EMBL:AL109950) Streptomyces coelicolor hypothetical 25.2 kD protein SCJ4.24c, 242 aa; fasta scores: opt: 725 z-score: 841.0 E(): 0; 47.8% identity in 245 aa overlap, to TR:CAC01591 (EMBL:AL391041) Streptomyces coelicolor putative secreted protein SC8A11.19c, 269 aa; fasta scores: opt: 1213 z-score: 1236.7 E(): 0; 74.2% identity in 248 aa overlap and to TR:Q98LL5 (EMBL:AP002996) Rhizobium loti MLR0977 protein, 252 aa; fasta scores: opt: 944 Z-score: 1074.9 E(): 3.1e-52; 58.607% identity in 244 aa overlap 3 1 21 16 [Reporter]SCO2112 (7K16)_PCR/1463 [Gene]SCO2112/1340 NC_003888 SC6E10.06c, hypothetical protein, len: 215 aa; unknown function, similar to hypothetical proteins e.g. TR:O32103 (EMBL:Z99120) Bacillus subtilis hypothetical protein (198 aa), fasta scores; opt: 522 z-score: 625.8 E(): 1.5e-27, 40.6% identity in 192 aa overlap. Shares a short sequence motif from aa 150-160 with many oxidoreductases (sulfite oxidases and nitrate reductases). An alternative start codon is present at codon 5 4 2 16 15 [Reporter]SCO5302 (8D11)_PCR/3872 [Gene]SCO5302/3519 NC_003888 SC6G9.31, probable integral membrane cell-cycle protein, len: 446 aa; similar to members of the ftsW (Escherichia coli cell division)/rodA (Escherichia coli cell shape)/spoVE (Bacillus subtilis spore formation) family e.g. SW:SP5E_BACSU (EMBL:X51419), spoVE, Bacillus subtilis stage V sporulation protein E (366 aa), fasta scores; opt: 461 z-score: 525.9 E(): 5.8e-22, 33.4% identity in 371 aa overlap. Highly similar to proteins from mycobacteria e.g. TR:FTSW_MYCLE (EMBL:Z70722), ftsW, Mycobacterium leprae probable cell division protein (465 aa) (46.5% identity in 430 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF01098 FTSW_RODA_SPOVE, Cell cycle protein 4 2 16 14 [Reporter]SCO6980 (9P7)_PCR/3871 [Gene]SCO6980/3518 NC_003888 SC8F11.06, probable ABC transport protein, membrane component, len: 359 aa. Similar to several including: Escherichia coli SW:MGLC_ECOLI(EMBL:M59444) galactoside transport system permease protein, MglC (336 aa), fasta scores opt: 455 z-score: 517.3 E(): 2.2e-21 29.2% identity in 329 aa overlap and several examples from Streptomyces coelicolor e.g. TR:CAB60177(EMBL:AL132824) putative ABC transport system sugar permease, SCAH10.23 (346 aa), fasta scores opt: 1426 z-score: 1605.5 E():0 62.2% identity in 336 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 2 16 13 [Reporter]SCO2508 (10L7)_PCR/3870 [Gene]SCO2508/3517 NC_003888 SCC121.11, probable metal uptake regulation protein, len: 139 aa; similar to TR:O05839 (EMBL:Z95208) Mycobacterium tuberculosis cosmid Y27 Fur, 130 aa; fasta scores: opt: 546 z-score: 663.0 E(): 1.5e-29; 61.8% identity in 123 aa overlap and to SW:FUR_NEIGO (EMBL:L11361) Neisseria gonorrhoeae ferric uptake regulation protein (ferric uptake regulator) Fur, 144 aa; fasta scores: opt: 273 z-score: 339.0 E(): 1.7e-11; 35.5% identity in 110 aa overlap. Contains Pfam match to entry PF01475 FUR 4 2 16 12 [Reporter]SCO2542 (11H7)_PCR/3869 [Gene]SCO2542/3516 NC_003888 SCC77.09c, possible glucarate dehydratase, len: 431 aa. Similar to many glucarate dehydratases and (less similar) to many members of the mandelate racemase / muconate lactonizing enzyme family: Escherichia coli SW:GUD1_ECOLI(EMBL:AE000362) probable glucarate dehydratase 1 (EC 4.2.1.40) (446 aa), fasta scores opt: 673 z-score: 769.9 E(): 0 37.3% identity in 440 aa overlap and Rhodococcus opacus TR:O67985(EMBL:AF003948) chloromuconate cycloisomerase (373 aa), fasta scores opt: 271 z-score: 312.8 E(): 4.9e-10 26.2% identity in 416 aa overlap. Contains a Pfam match to entry PF01188 MR_MLE. 4 2 16 11 [Reporter]SCO4458 (12D7)_PCR/3868 [Gene]SCO4458/3515 NC_003888 SCD65.01c, possible lipoprotein (fragment), len: >146 aa; similar to TR:Q55943 (EMBL:D64005) Synechocystis sp. hypothetical 22.9 kDa protein SLL0788, 196 aa; fasta scores: opt: 203 z-score: 217.8 E(): 0.00012; 33.9% identity in 118 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence,SCD6.36c, unknown (fragment), len: > 107 aa 4 2 16 10 [Reporter]SCO3435 (13P3)_PCR/3867 [Gene]SCO3435/3514 NC_003888 SCE36.02, probable transcriptional regulator, len: 295 aa; similar to many e.g. SW:GLTC_BACSU glutamate biosynthesis transcriptional regulator from Bacillus subtilis (300 aa) fasta scores; opt: 456, z-score: 520.3, E(): 1.2e-21, (31.9% identity in 288 aa overlap). Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix proteins, lysR family and Prosite match to PS00044 Bacterial regulatory proteins, lysR family signature. 4 2 16 9 [Reporter]SCO3582 (14L3)_PCR/3866 [Gene]SCO3582/3513 NC_003888 SCH66.03, putative secreted protein, len: 309 aa; similar to many e.g. TR:O69651 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (319 aa) fasta scores; opt: 610, z-score: 716.1, E(): 1.5e-32, (51.5% identity in 307 aa overlap). Contains possible N-terminal region signal peptide sequence 3 1 21 15 [Reporter]SCO6850 (8G16)_PCR/1462 [Gene]SCO6850/1339 NC_003888 SC7F9.02c, unknown, len: 259 aa. Similar to other hypothetical proteins predicted to be encoded by CDS located on this cosmid: SC7F9.04c (273 aa), fasta scores opt: 442 z-score: 398.4 E(): 9.8e-17 33.7% identity in 255 aa overlap and SC7F9.06c (267 aa), fasta scores opt: 385 z-score: 348.6 E(): 5.8e-14 34.6% identity in 257 aa overlap 4 2 16 8 [Reporter]SCO4956 (15H3)_PCR/3865 [Gene]SCO4956/3512 NC_003888 2SCK31.16, probable peptide methionine sulfoxide reductase, len: 172 aa; similar to SW:PMSR_ECOLI (EMBL:M89992) Escherichia coli peptide methionine sulfoxide reductase MsrA, 211 aa; fasta scores: opt: 437 z-score: 538.0 E(): 2e-22; 44.2% identity in 165 aa overlap. Contains Pfam match to entry PF01625 PMSR, Peptide methionine sulfoxide reductase 3 1 21 13 [Reporter]SCO1920 (10O12)_PCR/1460 [Gene]SCO1920/1338 NC_003888 SCC22.02c, conserved hypothetical protein, len: 156aa; similar to many other hypothetical protein eg. TR:O53156 (EMBL:AL021184) hypothetical protein from Mycobacterium tuberculosis (162 aa) fasta scores; opt: 569, z-score: 685.8, E(): 7.2e-31, (56.3% identity in 158 aa overlap) and to SW:NIFU_BACSU (EMBL:Z99120) Bacillus subtilis NifU-like protein NifU, 147 aa; fasta scores: opt: 439 Z-score: 527.7 E(): 9.3e-22; 45.517% identity in 145 aa overlap 4 2 16 7 [Reporter]SCO0288 (16D3)_PCR/3864 [Gene]SCO0288/3511 NC_003888 SCF85.16, unknown, len: 146 aa. 3 1 21 12 [Reporter]SCO4576 (11K12)_PCR/1459 [Gene]SCO4576/1337 NC_003888 SCD16A.07, unknown, len: 199 aa; weakly similar to others eg. TR:Q55360 (EMBL:D64003) hypothetical protein from Synechocystis sp. strain PCC6803 (190 aa) fasta scores; opt: 168, z-score: 209.8, E(): 0.00023, (26.6% identity in 177 aa overlap). 4 2 16 6 [Reporter]SCO5034 (16P23)_PCR/3863 [Gene]SCO5034/3510 NC_003888 SCK7.07c, possible integral membrane protein, len: 778 aa; similar to TR:AAF74504 (EMBL:M34538) Streptomyces clavuligerus hypothetical 42.6 kDa protein (fragment) 407 aa; fasta scores: opt: 509 z-score: 559.6 E(): 1.1e-23; 31.5% identity in 413 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 21 11 [Reporter]SCO4484 (12G12)_PCR/1458 [Gene]SCO4484/1336 NC_003888 SCD69.04, possible integral membrane protein, len: 120 aa; similar to TR:O85187 (EMBL:AF051158) Vibrio cholerae sodium-proton antiporter affecting protein NhaA, 382 aa; fasta scores: opt: 116 z-score: 146.3 E(): 1.1; 32.2% identity in 118 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 21 10 [Reporter]SCO3012 (13C12)_PCR/1457 [Gene]SCO3012/1335 NC_003888 SCE33.14c, probable two-component system histidine kinase, len: 688 aa; similar to TR:O05890 (EMBL:Z95121) Mycobacterium tuberculosis MtrB, 567 aa; fasta scores: opt: 1355 z-score: 1302.5 E(): 0; 48.9% identity in 515 aa overlap and to SW:AFQ2_STRCO (EMBL:D10654) Streptomyces coelicolor sensor protein AfsQ2 (EC 2.7.3.-) fasta scores: opt: 560 z-score: 542.0 E(): 9.8e-23; 31.1% identity in 460 aa overlap. Contains Pfam matches to entries PF00672 DUF5, Domain found in bacterial signal proteins and PF00512 signal, Histidine kinase. Contains possible hydrophobic membrane spanning regions 3 1 21 9 [Reporter]SCO3176 (14O8)_PCR/1456 [Gene]SCO3176/1334 NC_003888 SCE87.27c, possible membrane protein, len: 583 aa. Contains possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence. 3 1 21 8 [Reporter]SCO0502 (15K8)_PCR/1455 [Gene]SCO0502/1333 NC_003888 SCF34.21, possible membrane protein, len: 144 aa; unknown function, probable CDS suggested by GC frameplot and positional base preference. Contains hydrophobic, possible membrane-spanning regions 3 1 21 7 [Reporter]SCO1741 (16G8)_PCR/1454 [Gene]SCO1741/1332 NC_003888 SCI11.30c, probable secreted serine protease, len: 360 aa; highly similar to many serine proteases e.g. SW:PRTD_STRGR (EMBL:L29019), sprD, Streptomyces griseus serine protease D precursor (392 aa), fasta scores; opt: 1703 z-score: 1655.9 E(): 0, 67.1% identity in 392 aa overlap. Similar to S.coelicolor SCI11.35c, sal, secreted serine protease (300 aa) (52.2% identity in 278 aa overlap). Contains a probable N-terminal signal sequence. Contains probable coiled-coil from 84 to 119 (36 residues) (Max score: 1.468, probability 0.91). Contains Pfam match to entry PF00089 trypsin, Trypsin, PS00134 Serine proteases, trypsin family, histidine active site and PS00135 Serine proteases, trypsin family, serine active site 3 1 21 6 [Reporter]SCO0881 (17C8)_PCR/1453 [Gene]SCO0881/1331 NC_003888 SCM1.14c, unknown, len: 321 aa. Highly similar to another protein of undefined function from Streptomyces coelicolor TR:CAB56728(EMBL:AL121600) hypothetical 34.3 KD protein SCF76.08C (325 aa), fasta scores opt: 978 z-score: 1133.4 E(): 0 47.2% identity in 326 aa overlap 3 1 21 5 [Reporter]SCO1045 (18O4)_PCR/1452 [Gene]SCO1045/1330 NC_003888 SCG20A.25, possible metal associated protein, len: 77 aa; similar to SW:COPP_HELFE (EMBL:AJ001932) Helicobacter felis cop associated protein (copper ion binding protein) CopP, 66 aa; fasta scores: opt: 153 z-score: 210.7 E(): 0.00029; 40.7% identity in 59 aa overlap and to TR:Q9RDJ5 (EMBL:AL136519) Streptomyces coelicolor putative regulator (fragment) SCC57A.01, >47 aa; fasta scores: opt: 146 z-score: 186.0 E(): 9.3e-05; 53.2% identity in 47 aa overlap. Contains Pfam match to entry PF00403 HMA, Heavy-metal-associated domain 4 2 16 5 [Reporter]SCO2996 (17L23)_PCR/3862 [Gene]SCO2996/3509 NC_003888 SCE99.03, possible ABC transporter ATP-binding protein, len: 288 aa; similar to SW:RFB1_KLEPN (EMBL:L31775) Klebsiella pneumoniae O-antigen export system ATP-binding protein RfbB, 246 aa; fasta scores: opt: 550 z-score: 504.2 E(): 1.5e-20; 40.2% identity in 209 aa overlap and to TR:Q9X7V3 (EMBL:AL049085) Streptomyces coelicolor putative ABC transporter ATP-binding component SC6A5.07, 253 aa; fasta scores: opt: 1381 z-score: 963.7 E(): 0; 82.5% identity in 251 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 4 2 16 4 [Reporter]SCO0542 (18H23)_PCR/3861 [Gene]SCO0542/3508 NC_003888 SCF11.22, hypothetical regulatory protein, len: 877 aa; similar to various hypothetical proteins, e.g. TR:CAB44382 (EMBL:AL078610) Streptomyces coelicolor hypothetical 66.2 KD protein, 634 aa; fasta scores: opt: 1072 z-score: 1125.7 E(): 0; 38.2% identity in 678 aa overlap and to SW:RSBU_BACSU (EMBL:L35574) Bacillus subtilis sigma factor sibG regulation protein RsbU, 335 aa; fasta scores: opt: 211 z-score: 225.9 E(): 3.2e-05; 25.6% identity in 250 aa overlap. Contains Pfam matches to entries PF00989 PAS, PAS domain and PF01590 GAF, GAF domain 4 2 16 3 [Reporter]SCO1319 (19D23)_PCR/3860 [Gene]SCO1319/3507 NC_003888 2SCG61.01, unknown, partial CDS, len: >174 aa,SCBAC36F5.30, putative membrane protein (fragment), len: >199 aa; similar to N-terminal region of TR:O88067 (EMBL:AL031541) Streptomyces coelicolor putative membrane protein SCI35.32c, 319 aa; fasta scores: opt: 227 Z-score: 214.6 bits: 47.2 E(): 0.00024; 33.333% identity in 162 aa overlap and to N-terminal region of Streptomyces coelicolor hypothetical protein SCBAC36F5.29, 327 aa; fasta scores: opt: 337 Z-score: 288.4 bits: 60.9 E(): 2.4e-10; 47.291% identity in 203 aa overlap. Contains possible hydrophobic membrane spanning region 4 2 16 2 [Reporter]SCO7499 (20L19)_PCR/3859 [Gene]SCO7499/3506 NC_003888 SCBAC17A6.32c, possible racemase, len: 241aa; similar to SW:Q00924 (HYUE_PSESN) hydantoin racemase from the native plasmid of Pseudomonas sp. strain NS671 (249 aa) fasta scores: opt: 564, Z-score: 617.1, 39.574% identity (39.744% ungapped) in 235 aa overlap 4 2 15 22 [Reporter]SCO1219 (1L15)_PCR/3857 [Gene]SCO1219/3505 NC_003888 2SCG58.19c, possible integral membrane protein, len: 131 aa. Contains possible hydrophobic membrane spanning regions 4 2 15 21 [Reporter]SCO5440 (2H15)_PCR/3856 [Gene]SCO5440/3504 NC_003888 SC6A11.16c, glgBI, 1,4-alpha-glucan branching enzyme, len: 774 aa; previously sequenced and characterised as TR:Q59833 (EMBL:X83397). Also strongly similar to many others e.g. SW:P07762 (GLGB_ECOLI) 1,4-alpha-glucan branching enzyme from Escherichia coli (728 aa) fasta scores: opt: 2149, z-score: 2347.4, E(): 0, 50.5% identity in 711 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase 4 2 15 20 [Reporter]SCO2233 (3D15)_PCR/3855 [Gene]SCO2233/3503 NC_003888 SC10B7.28, probable integral membrane protein, len: 287 aa; similar to TR:Q9XAN7 (EMBL:AL079355) Streptomyces coelicolor putative membrane protein SC4C6.03c, 271 aa; fasta scores: opt: 489 z-score: 586.3 E(): 2.8e-25; 38.0% identity in 255 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 21 4 [Reporter]SCO4995 (19K4)_PCR/1451 [Gene]SCO4995/1329 NC_003888 2SCK36.18c, putative membrane protein, len: 420 aa. Contains possible hydrophobic membrane spanning region 4 2 15 19 [Reporter]SCO7026 (4P11)_PCR/3854 [Gene]SCO7026/3502 NC_003888 SC1H10.15, unknown, len: 147 aa. Similar to several proteins of unknown function e.g. Bacillus subtilis TR:O32183(EMBL:Z99120) hypothetical protein, YusQ (127 aa), fasta scores opt: 227 z-score: 315.9 E(): 3.6e-10 27.9% identity in 122 aa overlap. 3 1 21 3 [Reporter]SCO4000 (20G4)_PCR/1450 [Gene]SCO4000/1328 NC_003888 2SC10A7.04c, unknown, len: 159 aa 4 2 15 18 [Reporter]SCO2101 (5L11)_PCR/3853 [Gene]SCO2101/3501 NC_003888 SC4A10.34, probable carotenoid dehydrogenase (putative secreted protein), len: 506 aa; similar to many bacterial and fungal carotenoid dehydrogenases e.g. SW:CRTD_RHOSH (EMBL:X63204) CrtD, Rhodobacter sphaeroides methoxyneurosporene dehydrogenase (495 aa), fasta scores; opt: 470 z-score: 505.2 E(): 8.1e-21, 29.2% identity in 506 aa overlap. Shows very weak similarity to TR:Q9ZBG4 (EMBL:AL035206) S. coelicolor possible dehydrogenase (472 aa) (28.1% identity in 523 aa overlap). Contains possible N-terminal region signal peptide sequence 3 1 21 2 [Reporter]SCO5990 (20O24)_PCR/1449 [Gene]SCO5990/1327 NC_003888 SCBAC16H6.25c, unknown, len: 78 aa: no significant database matches. 4 2 15 17 [Reporter]SCO5802 (6H11)_PCR/3852 [Gene]SCO5802/3500 NC_003888 SC4H2.23, probable ATP-dependent helicase, len: 664 aa; similar to e.g. DING_ECOLI P27296 probable ATP-dependent helicase dinG (716 aa), fasta scores opt: 272 z-score: 409.8 E(): 1.3e-15, 26.5% identity in 686 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 20 22 [Reporter]SCO1749 (1O20)_PCR/1447 [Gene]SCO1749/1326 NC_003888 2SCI34.02, conserved hypothetical protein, len: 146 aa; similar to TR:O06229 (EMBL:Z95388) Mycobacterium tuberculosis hypothetical 27.6 kDa protein MTCY270.21, 241 aa; fasta scores: opt: 311 z-score: 378.3 E(): 1.3e-13; 41.8% identity in 141 aa overlap 3 1 20 21 [Reporter]SCO1255 (2K20)_PCR/1446 [Gene]SCO1255/1325 NC_003888 2SCG18.02, mug, G/U mismatch-specific DNA glycosylase, len: 160 aa; highly similar to SW:MUG_ECOLI (EMBL:U28379) Escherichia coli mismatch-specific DNA glycosylase (EC 3.2.2.-) Mug, 168 aa; fasta scores: opt: 448 z-score: 564.7 E(): 5.7e-24; 44.7% identity in 152 aa overlap 3 1 20 20 [Reporter]SCO7377 (3G20)_PCR/1445 [Gene]SCO7377/1324 NC_003888 SC10G8.04c, unknown, len: 85 aa. 3 1 20 19 [Reporter]SCO5622 (4C20)_PCR/1444 [Gene]SCO5622/1323 NC_003888 SC2E1.39, possible transcriptional regulator, len: 185 aa; equivalent to Streptomyces roseosporus TR:P72569 (EMBL:U58281) tetR homologue WhiR (179 aa) (86.6% identity in 179 aa overlap) and similar to several putative transcriptional regulators of the tetR family e.g. TR:E1247678 putative transcriptional regulator SC10A5.11 (S.coelicolor) (198 aa), fasta scores; opt: 434 z-score: 720.5 E(): 6.8e-33, 41.5% identity in 171 aa overlap. Contains probable helix-turn-helix motif at aa 29 to 50 (Score 1618, +4.70 SD) and Pfam match to entry tetR PF00440, Bacterial regulatory proteins, tetR family, score 29.18 3 1 20 18 [Reporter]SCO6693 (5O16)_PCR/1443 [Gene]SCO6693/1322 NC_003888 SC4C6.03c, possible membrane protein, len: 271 aa; similar to TR:Q9KZ06 (EMBL:AL355752) Streptomyces coelicolor putative integral membrane protein SC10B7.28, 287 aa; fasta scores: opt: 489 Z-score: 561.8 E(): 1.2e-23; 36.471% identity in 255 aa overlap. Unknown function, probable CDS suggested by positional base preference, GC frame plot and amino acid composition. Contains hydrophobic, possible membrane-spanning regions 3 1 20 17 [Reporter]SCO2181 (6K16)_PCR/1442 [Gene]SCO2181/1321 NC_003888 SC5F7.20, sucB, possible dihydrolipoamide succinyltransferase, len: 590 aa; similar to many egs. TR:AAC23517 (EMBL:AF068740) dihydrolipoamide succinyltransferase from Pseudomonas ovalis (407 aa) fasta scores; opt: 847, z-score: 602.5, E(): 3.2e-26, (41.9% identity in 453 aa overlap) and SW:ODO2_MYCTU dihydrolipoamide succinyltransferase from Mycobacterium tuberculosis (553 aa) fasta scores; opt: 1232, z-score: 866.2, E(): 0, (56.2% identity in 596 aa overlap). Contains two Pfam matches to entry PF00364 biotin_lipoyl, Biotin-requiring enzymes, Pfam match to entry PF00198 2-oxoacid_dh, 2-oxo acid dehydrogenases acyltransferase (catalytic domain) and two Prosite matches to PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. 3 1 20 16 [Reporter]SCO2658 (7G16)_PCR/1441 [Gene]SCO2658/1320 NC_003888 SC6D10.01, possible sugar-binding protein (partial), len: > 273 aa; similar to TR:CAB49101 (EMBL:AJ248283) Pyrococcus abyssi maltose-binding periplasmic protein precursor MalE, 453 aa; fasta scores: opt: 246 z-score: 288.7 E(): 1.1e-08; 24.0% identity in 271 aa overlap and to SW:MALX_STRPN (EMBL:L08611) Streptococcus pneumoniae maltose/maltodextrin-binding protein precursor MalX, 423 aa; fasta scores: opt: 184 z-score: 217.6 E(): 0.0001; 22.9% identity in 279 aa overlap,SC8E4A.28, possible secreted sugar-binding protein (fragment), len: >205 aa; similar to TR:O87856 (EMBL:AL031013) Streptomyces coelicolor putative secreted sugar binding protein SC8A6.22, 438 aa; fasta scores: opt: 314 z-score: 373.2 E(): 2.5e-13; 38.8% identity in 206 aa overlap and to TR:O51923 (EMBL:AF012836) Thermococcus litoralis trehalose/maltose binding protein MalE, 450 aa; fasta scores: opt: 272 z-score: 323.9 E(): 1.4e-10; 31.7% identity in 183 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal sequence peptide 3 1 20 15 [Reporter]SCO5237 (8C16)_PCR/1440 [Gene]SCO5237/1319 NC_003888 SC7E4.34c, probable oxidoreductase, len: 250 aa; highly similar to TR:Q9RKS8 (EMBL:AL132824) Streptomyces coelicolor reductase SCAH10.29, 262 aa; fasta scores: opt: 807 z-score: 863.1 E(): 0; 52.4% identity in 252 aa overlap and to SW:VER1_ASPPA (EMBL:M91369) Aspergillus parasiticus versicolorin reductase (EC 1.1.-.-) Ver1, 262 aa; fasta scores: opt: 469 z-score: 506.7 E(): 9.4e-21; 39.2% identity in 255 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus 3 1 20 14 [Reporter]SCO5408 (9O12)_PCR/1439 [Gene]SCO5408/1318 NC_003888 SC8F4.12c, hypothetical protein, len: 239 aa; weakly similar to many e.g. TR:AAF10203 (EMBL:AE00192) hypothetical protein from Deinococcus radiodurans (254 aa) fasta scores; opt: 441, z-score: 528.2, E(): 5.1e-22, 33.2% identity in 202 aa overlap. Also weakly similar to the neighbouring hypothetical CDSs SC8F4.10c and SC8F4.11c 3 1 20 13 [Reporter]SCO2588 (10K12)_PCR/1438 [Gene]SCO2588/1317 NC_003888 SCC123.26, possible integral membrane protein, len: 719 aa. Contains possible membrane spanning hydrophobic domains. 3 1 20 12 [Reporter]SCO2343 (11G12)_PCR/1437 [Gene]SCO2343/1316 NC_003888 SCC8A.01, possible acetyltransferase, len: 177 aa; similar to many of undefined function e.g. TR:CAB59671 (EMBL:AL132674) putative acetyltransferase from Streptomyces coelicolor (161 aa) fasta scores; opt: 194, z-score: 245.6, E(): 3.2e-06, 33.3% identity in 165 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family, score 44.30, E-value 2.7e-09. 3 1 20 11 [Reporter]SCO4185 (12C12)_PCR/1436 [Gene]SCO4185/1315 NC_003888 SCD66.22, conserved hypothetical protein, len: 336 aa; highly similar to TR:Q54237 (EMBL:D63706) Streptomyces griseus ORF4 hypothetical protein, 328 aa; fasta scores: opt: 1654 z-score: 1801.0 E(): 0; 78.6% identity in 322 aa overlap and N-terminal domain identical to TR:Q9Z4H1 (EMBL:D63677) Streptomyces coelicolor ORF4 hypothetical protein (fragment), 142 aa. Contains 3x degenerate repeat at N-terminal domain: PS(H/Q) 3 1 20 10 [Reporter]SCO3100 (13O8)_PCR/1435 [Gene]SCO3100/1314 NC_003888 SCE41.09c, conserved hypothetical protein, len: 325 aa; similar to SW:YBL1_STRCI (EMBL:D00937) Streptomyces cacaoi hypothetical 26.1 kDa protein in BlaB 3' region, 242 aa; fasta scores: opt: 1157 z-score: 1300.4 E(): 0; 72.7% identity in 242 aa overlap 3 1 20 9 [Reporter]SCO3174 (14K8)_PCR/1434 [Gene]SCO3174/1313 NC_003888 SCE87.25c, possible exodeoxyribonuclease (EC 3.1.11.2) (putative secreted protein), len: 274 aa. Highly similar to several e.g. Bacillus subtilis SW:EXOA_BACSU (EMBL:D26185) exodeoxyribonuclease (EC 3.1.11.2) (252 aa), fasta scores opt: 592 z-score: 694.3 E(): 2.7e-31 38.6% identity in 267 aa overlap and Methanobacterium thermoautotrophicum TR:O26314 (EMBL:AE000808) exodeoxyribonuclease (257 aa), fasta scores opt: 674 z-score: 789.1 E():0 41.6% identity in 262 aa overlap. Also similar to Pseudomonas aeruginosa TR:Q51380 (EMBL:U38241) catabolite repression control protein (259 aa), fasta scores opt: 601 z-score: 704.5 E(): 7.2e-32 39.3% identity in 267 aa overlap. Contains a Pfam match to entry PF01260 AP_endonucleas1, AP endonuclease family 1. Note that the translational start site may be located at residue 8. Contains possible N-terminal region signal peptide sequence 3 1 20 8 [Reporter]SCO0046 (15G8)_PCR/1433 [Gene]SCO0046/1312 NC_003888 SCJ4.27, possible hydrolase, len: 220 aa; similar to SW:ACPD_ECOLI acyl carrier protein phosphodiesterase (200 aa) fasta scores; opt: 272, z-score: 336.5, E(): 2.2e-11, (33.2% identity in 193 aa overlap) 3 1 20 7 [Reporter]SCO5598 (16C8)_PCR/1432 [Gene]SCO5598/1311 NC_003888 SC2E1.15, sip3, probable signal peptidase I, len: 336 aa; identical to, but longer than, S. lividans Sip TR:E1228248 (EMBL:Y09474) signal peptidase I (291 aa), fasta scores; opt: 1997 z-score: 2287.4 E(): 0, 99.7% identity in 291 aa overlap, and similar to many others e.g. LEP_PHOLA signal peptidase I (EC 3.4.21.89) (203 aa), fasta scores; opt:222 z-score: 404.9 E(): 2.6e-15, 37.9% identity in 232 aa overlap. Contains PS00761 Signal peptidases I signature 3, 2x Pfam match to entry signal_pept_I PF00461, Signal peptidases I, scores 48.83 and 46.79 and probable transmembrane domain. Also similar to surrounding genes sip1 (E(): 7.6e-20, 35.6% identity in 264 aa overlap), sip2 (E(): 0, 56.3% identity in 245 aa overlap) and sip4 (E(): 6.6e-15, 36.3% identity in 237 aa overlap). Note that a more upstream start was chosen due to positional base preference. Contains possible hydrophobic membrane spanning regions 3 1 20 6 [Reporter]SCO1046 (17O4)_PCR/1431 [Gene]SCO1046/1310 NC_003888 SCG20A.26, probable metal transporter ATPase, len: 753 aa; similar to SW:ATCU_RHIME (EMBL:AF129004) Rhizobium meliloti copper-transporting ATPase (EC 3.6.1.36) ActP, 827 aa; fasta scores: opt: 1748 z-score: 1773.4 E(): 0; 42.4% identity in 767 aa overlap and to TR:Q9RDJ4 (EMBL:AL136519) Streptomyces coelicolor putative cation-transporting P-type ATPase SCC57A.02, 760 aa; fasta scores: opt: 3484 z-score: 2834.6 E(): 0; 73.9% identity in 761 aa overlap. Contains Pfam matches to entries PF00403 HMA, Heavy-metal-associated domain and PF00122 E1-E2_ATPase, E1-E2 ATPase and matches to Prosite entries PS01047 Heavy-metal-associated domain, PS00154 E1-E2 ATPases phosphorylation site and PS01229 Hypothetical cof family signature 2. Contains also possible hydrophobic membrane spanning regions 3 1 20 5 [Reporter]SCO0591 (18K4)_PCR/1430 [Gene]SCO0591/1309 NC_003888 SCF55.15, probable lysozyme precursor, len: 279 aa; similar to SW:LYCM_STRGL (EMBL:M30645) Streptomyces globisporus lysozyme M1 precursor (EC 3.2.1.17) (1,4-beta-N-acetylmuramidase M1) Acm, 294 aa; fasta scores: opt: 837 z-score: 971.2 E(): 0; 44.4% identity in 284 aa overlap. Contains match to Pfam entry PF01183 Glyco_hydro_25, Glycosyl hydrolases family 25 and two matches to Prosite entries PS00953 Glycosyl hydrolases family 25 active sites signature and PS00017 ATP/GTP-binding site motif A (P-loop). Contains also possible N-terminal signal sequence 3 1 20 4 [Reporter]SCO4037 (19G4)_PCR/1429 [Gene]SCO4037/1308 NC_003888 2SCD60.03c, possible small membrane protein, len: 58 aa. Contains possible hydrophobic membrane spanning region 3 1 20 3 [Reporter]SCO6297 (20C4)_PCR/1428 [Gene]SCO6297/1307 NC_003888 SCBAC8D1.10c, probable 3-hydroxyacyl-CoA dehydrogenase, len: 318 aa; similar to TR:Q9HTH8 (EMBL:AE004951) Pseudomonas aeruginosa probable 3-hydroxyacyl-CoA dehydrogenase PA5386, 321 aa; fasta scores: opt: 1055 Z-score: 1152.5 bits: 221.5 E(): 1.3e-56; 54.072% identity in 307 aa overlap. Contains Pfam matches to entries PF02737 3HCDH_N, 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain and Pfam match to entry PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain 3 1 20 2 [Reporter]SCO7479 (20K24)_PCR/1427 [Gene]SCO7479/1306 NC_003888 SCBAC17A6.12c, possible integral membrane protein, len: 387aa: similar to many eg. TR:Q9FAT6 (EMBL:AB038042) Mrx hypothetical protein associated with macrolide 2'-phosphotransferase I in Escherichia coli plasmid pTZ3509 (412 aa) fasta scores; opt: 316, Z-score: 317.3, 29.873% identity (31.978% ungapped) in 395 aa overlap. Contains multiple possible membrane-spanning hydrophobic regions. 3 1 19 22 [Reporter]SCO1356 (1K20)_PCR/1425 [Gene]SCO1356/1305 NC_003888 2SCG61.38, possible iron sulphur protein (putative secreted protein), len: 165 aa; similar to TR:Q9S237 (EMBL:AL109848) putative iron sulphur protein from Streptomyces coelicolor (131 aa) fasta scores; opt: 444, z-score: 411.3, E(): 1.9e-15, 53.4% identity in 116 aa overlap. Contains Pfam match to entry PF00355 Rieske, Rieske [2Fe-2S] domain and Prosite match to PS00200 Rieske iron-sulfur protein signature 2. Contains possible N-terminal region signal peptide sequence 3 1 19 21 [Reporter]SCO0658 (2G20)_PCR/1424 [Gene]SCO0658/1304 NC_003888 SCF91.18, gvpK2, possible gas vesicle synthesis protein, len: 100 aa. Highly similar to many other gas vesicle synthesis proteins including: Halobacterium salinarium SW:GVK2_HALSA(EMBL:X64730) gas vesicle synthesis protein GvpK (119 aa), fasta scores opt: 163 z-score: 224.6 E(): 3.9e-05 43.3% identity in 67 aa overlap and Bacillus megaterium TR:O68670(EMBL:AF053765) gas vesicle protein GvpK (94 aa), fasta scores opt: 302 z-score: 402.9 E(): 4.6e-15 53.3% identity in 92 aa overlap. Also highly similar to Streptomyces coelicolor TR:Q9ZC05 (EMBL:AL033505) putative gas vesicle synthesis protein GvpK (98 aa), fasta scores opt: 370 z-score: 489.0 E(): 7.3e-20 66.3% identity in 92 aa overlap. 3 1 19 20 [Reporter]SCO7666 (3C20)_PCR/1423 [Gene]SCO7666/1303 NC_003888 SC4C2.01, possible zinc-binding oxidoreductase (fragment), len: >133 aa; similar to TR:CAC14345 (EMBL:AL445945) Streptomyces coelicolor putative zinc-binding oxidoreductase 3SCF60.12, 313 aa; fasta scores: opt: 218 z-score: 267.4 E(): 2.3e-07; 37.8% identity in 127 aa overlap,SC10F4.39, possible zinc-binding oxidoreductase (fragment), len: >218 aa; similar to TR:Q9L2A5 (EMBL:AL137242) Streptomyces coelicolor putative zinc-binding oxidoreductase SC8F4.21, 339 aa; fasta scores: opt: 465 z-score: 527.6 E(): 7.5e-22; 41.7% identity in 211 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenase 3 1 19 19 [Reporter]SCO7228 (4O16)_PCR/1422 [Gene]SCO7228/1302 NC_003888 SC2H12.27c, possible polysaccharide lyase, len: 554 aa. Similar to regions within several pectate lyases e.g. Erwinia chrysanthemi SW:PELX_ERWCH(EMBL:M62739) exopolygalacturonate lyase precursor (EC 4.2.2.9), PelX (749 aa), fasta scores opt: 441 z-score: 462.5 E(): 2.7e-18 33.2% identity in 455 aa overlap. 3 1 19 18 [Reporter]SCO7690 (5K16)_PCR/1421 [Gene]SCO7690/1301 NC_003888 SC4C2.25, probable ABC transporter ATP-binding protein, len: 587 aa; similar to SW:YD49_MYCTU (EMBL:Z75555) Mycobacterium tuberculosis hypothetical ABC transporter ATP-binding protein RV1349, 579 aa; fasta scores: opt: 1144 z-score: 1191.3 E(): 0; 36.8% identity in 590 aa overlap. Contains Pfam matches to entries PF00664 ABC_membrane, ABC transporter transmembrane region and PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00038 Myc-type, 'helix-loop-helix' dimerization domain signature, PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also contains possible hydrophobic membrane spanning regions 3 1 19 17 [Reporter]SCO7785 (6G16)_PCR/1420 [Gene]SCO7785/1300 NC_003888 SC5E9.33, possible transcriptional regulator, len: 206 aa; weakly similar to many eg. TR:Q9S250 (EMBL:AL096811) putative transcriptional regulator from Streptomyces coelicolor (213 aa) fasta scores; opt: 159, z-score: 199.4, E(): 0.0012, 25.7% identity in 171 aa overlap. Contains helix-turn-helix motif (Score 1164 (+3.15 SD)) at residue 33-54. Note possible alternative downstream start sites. 2 4 12 11 [Reporter]SCO4446 (12B1)_PCR/6803 [Gene]SCO4446/6199 NC_003888 SCD6.24, possible integral membrane protein, len: 405 aa; similar to TR:CAB72211 (EMBL:AL138851) Streptomyces coelicolor putative transport protein SCE59.24, 366 aa; fasta scores: opt: 204 z-score: 229.5 E(): 2.5e-05; 27.1% identity in 358 aa overlap. Contains possible hydrophobic membrane spanning regions 2 4 12 10 [Reporter]SCO3531 (12N21)_PCR/6802 [Gene]SCO3531/6198 NC_003888 SCE2.12, unknown, len: 259aa; similar to TR:Q9L1M6 (EMBL:AL138977) SC7F9.41 hypothetical protein from Streptomyces coelicolor (246 aa) fasta scores; opt: 405, Z-score: 481.6, E(): 3.4e-19, 35.622% identity (39.713% ungapped) in 233 aa overlap. 2 4 12 9 [Reporter]SCO1466 (13J21)_PCR/6801 [Gene]SCO1466/6197 NC_003888 SCL6.23c, possible integral membrane protein, len: 312 aa. Contains possible hydrophobic membrane spanning regions 2 4 12 8 [Reporter]SCO1586 (14F21)_PCR/6800 [Gene]SCO1586/6196 NC_003888 SCI35.08c, hypothetical protein, len: 146 aa; distant similarity to a hypothetical protein from Synechocystis sp. TR:Q55911 (EMBL:D64005) SLL0286 (171 aa), fasta scores; opt: 142 z-score: 208.1 E(): 0.00024, 33.9% identity in 127 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family, score 24.80, E-value 0.002 2 4 12 7 [Reporter]SCO0129 (15B21)_PCR/6799 [Gene]SCO0129/6195 NC_003888 SCJ21.10, unknown, len: 124 aa 2 4 12 6 [Reporter]SCO4963 (16N17)_PCR/6798 [Gene]SCO4963/6194 NC_003888 2SCK31.23, possible ABC transporter ATP-binding protein, len: 335 aa; similar to TR:CAC08315 (EMBL:AL392149) Streptomyces coelicolor putative ABC transport system ATP-binding protein SCD19A.14, 336 aa; fasta scores: opt: 1211 z-score: 1254.5 E(): 0; 58.2% identity in 330 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 2 4 12 4 [Reporter]SCO4941 (18F17)_PCR/6796 [Gene]SCO4941/6193 NC_003888 2SCK31.01c, hypothetical protein, len: 105 aa; similar to TR:Q9KZT7 (EMBL:AL353832) Streptomyces coelicolor hypothetical 20.2 kDa protein (fragment) SCE6.01c, 183 aa; fasta scores: opt: 191 z-score: 223.2 E(): 6.8e-05; 41.5% identity in 82 aa overlap 2 4 12 3 [Reporter]SCP1.303 (19B17)_PCR/6795 [Gene]SCP1.303/6192 NC_003888 SCP1.303, spore associated protein, SapE, len: 174aa; similar to many of undefined function eg. TR:Q9ZC37 (EMBL:AL031866) hypothetical protein from Yersinia pestis (180 aa) fasta scores; opt: 490, z-score: 646.6, E(): 1.5e-28, 47.8% identity in 157 aa overlap. Contains Pfam match to entry PF01575 MaoC_dehydratas, MaoC like domain. 2 4 12 2 [Reporter]SCO3975 (20J13)_PCR/6794 [Gene]SCO3975/6191 NC_003888 SCBAC25E3.12c, possible regulator, len: 212 aa: similar to TR:Q53897 (EMBL:X60316) AbaA regulatory protein from Streptomyces coelicolor (192 aa) fasta scores; opt: 225 , Z-score: 260.3, 33.333% identity (36.364% ungapped) in 156 aa overlap. 2 4 11 22 [Reporter]SCO1241 (1J9)_PCR/6792 [Gene]SCO1241/6190 NC_003888 2SCG1.16c, conserved hypothetical protein, len: 170 aa; similar to TR:Q9X7Q5 (EMBL:AL049587) Streptomyces coelicolor hypothetical 17.1 kD protein, 161 aa; fasta scores: opt: 269 z-score: 295.6 E(): 5.3e-09; 39.4% identity in 132 aa overlap 2 4 11 21 [Reporter]SCO5031 (2F9)_PCR/6791 [Gene]SCO5031/6189 NC_003888 SCK7.04c, ahpD, alkyl hydroperoxide reductase system hypothetical protein, len: 178 aa; identical to previously sequenced TR:Q9RN73 (EMBL:AF186371) Streptomyces coelicolor A3(2) AhpD, 178 aa 2 4 11 20 [Reporter]SCO4142 (3B9)_PCR/6790 [Gene]SCO4142/6188 NC_003888 SCD84.09c, pstS, phosphate-binding protein precursor, len: 370 aa; similar to TR:O86343 (EMBL:Z95209) Mycobacterium tuberculosis phosphate-binding protein 3 precursor PstS3 or PhoS2, 370 aa; fasta scores: opt: 941 z-score: 994.7 E(): 0; 42.0% identity in 374 aa overlap and to SW:PSTS_ECOLI (EMBL:K01992) Escherichia coli phosphate-binding periplasmic protein precursor PstS or PhoS, 346 aa; fasta scores: opt: 451 z-score: 481.3 E(): 2.4e-19; 31.2% identity in 321 aa overlap. Contains Pfam match to entry PF01449 PstS, Phosphate-binding protein, correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and to entry PS00038 Myc-type, 'helix-loop-helix' dimerization domain signature. Contains also possible N-terminal region signal peptide sequence 2 4 11 19 [Reporter]SCO6619 (4N5)_PCR/6789 [Gene]SCO6619/6187 NC_003888 SC1F2.16c, probable dehydrogenase, len: 276 aa; similar to many e.g. TR:O24479 (EMBL:U89510) B-keto acyl reductase from Hordeum vulgare (barley) (325 aa), fasta scores; opt: 316 z-score: 468.7 E(): 7.2e-19, 36.4% identity in 214 aa overlap and HETN_ANASP ketoacyl reductase hetN (287 aa), fasta scores; opt: 343 z-score: 416.6 E(): 5.8e-16, 36.8% identity in 220 aa overlap. Contains PS00061 Short-chain dehydrogenases reductases to entry PF00106 adh_short, Alcohol /other dehydrogenases, short chain type, score 166.30, E-value 5.1e-46 2 4 11 18 [Reporter]SCO6573 (5J5)_PCR/6788 [Gene]SCO6573/6186 NC_003888 SC3F9.08, probable secreted protein, len: 417 aa; some similarity to many eukaryotic myo-inositol-1-phosphate synthases e.g. INO1_SPIPO (510 aa), fasta scores; opt: 159 z-score: 190.1 E(): 0.0022, 28.1% identity in 423 aa overlap, and to Archaeoglobus fulgidus TR:O28480 (EMBL:AE000979) AF1794 (392 aa), fasta scores; opt: 546 z-score: 713.5 E(): 1.6e-32, 37.0% identity in 397 aa overlap. Contains possible N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 2 4 11 17 [Reporter]SCO2036 (6F5)_PCR/6787 [Gene]SCO2036/6185 NC_003888 SC4G6.05c, trpA, tryptophan synthase alpha subunit, len: 271aa; previously sequenced therefore identical (except for A>G conflict at first base) to TR:O68816 (EMBL:AF054585). Also similar to many others eg. SW:TRPA_THETH tryptophan synthase alpha chain from Thermus thermophilus (271 aa) fasta scores; opt: 711, z-score: 781.5, E(): 0, (47.6% identity in 248 aa overlap). Contains Pfam match to entry PF00290 trp_syntA, Tryptophan synthase alpha chain and Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 2 4 11 16 [Reporter]SCO7285 (7B5)_PCR/6786 [Gene]SCO7285/6184 NC_003888 SC5H1.07c, possible hydroxylase, len: 421 aa; weakly similar to many e.g. TR:P96555 (EMBL:AB000564) Sphingomonas sp. salicylate hydroxylase (395 aa), fasta scores; opt: 188 z-score: 216.6 E(): 9.6e-05, 26.5% identity in 359 aa overlap and TR:Q53657 (EMBL:D38214), Cts8, Streptomyces aureofaciens 6-hydroxylation enzyme of tetracycline (469 aa) (25.9% identity in 347 aa overlap). Weakly similar to SCE29.14c (EMBL:AL035707) S.coelicolor possible salicylate hydroxylase (420 aa) (29.3% identity in 372 aa overlap). Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase, score 42.50, E-value 9.2e-09 2 4 11 14 [Reporter]SCO2639 (9J1)_PCR/6784 [Gene]SCO2639/6183 NC_003888 SC8E4A.09c, possible RNA polymerase sigma factor, len: 208 aa; similar to TR:O53798 (EMBL:AL021958) Mycobacterium tuberculosis RNA polymerase sigma factor SigJ, 177 aa; fasta scores: opt: 221 z-score: 269.6 E(): 1.3e-07; 34.9% identity in 146 aa overlap and to SW:SIGV_BACSU (EMBL:U93874) Bacillus subtilis RNA polymerase sigma factor SigV, 166 aa; fasta scores: opt: 177 z-score: 218.8 E(): 8.9e-05; 28.2% identity in 142 aa overlap. Contains possible helix-turn-helix motif at residues 171..192 (+2.79 SD) 2 4 11 13 [Reporter]SCO6479 (10F1)_PCR/6783 [Gene]SCO6479/6182 NC_003888 SC9C7.15c, probable secreted peptidase, len: 529aa; similar to many eg. TR:Q54410(EMBL:L27466) secreted tripeptidyl aminopeptidase from Streptomyces lividans 66 (537 aa) fasta scores; opt: 1362, z-score: 1170.0, E(): 0, (43.2% identity in 526 aa overlap), has probable N-terminal signal sequence. Contains Pfam match to entry PF00561 abhydrolase, alpha /beta hydrolase fold. Also similar to TR:O69831 (EMBL:AL023517) from Streptomyces coelicolor cosmid 1B5 (543 aa) fasta scores; opt: 1205, z-score: 1105.7, E(): 0, (42.3% identity in 541 aa overlap). 2 4 11 12 [Reporter]SCO2703 (11B1)_PCR/6782 [Gene]SCO2703/6181 NC_003888 SCC61A.24c, possible membrane protein, len: 100 aa; similar to TR:CAB75306 (EMBL:AL139164) putative small secreted protein from Streptomyces coelicolor (75 aa) fasta scores; opt: 114, z-score: 155.1, E(): 0.35, 43.1% identity in 51 aa overlap. Contains possible hydrophobic membrane spanning region 2 4 11 11 [Reporter]SCO4672 (11N21)_PCR/6781 [Gene]SCO4672/6180 NC_003888 SCD40A.18c, possible secreted protein, len: 589 aa. Contains possible N-terminal region signal peptide sequence 2 4 11 10 [Reporter]SCO3527 (12J21)_PCR/6780 [Gene]SCO3527/6179 NC_003888 SCE2.08, unknown, len: 76aa; 2 4 11 9 [Reporter]SCO3708 (13F21)_PCR/6779 [Gene]SCO3708/6178 NC_003888 SCH35.16, possible integral membrane protein, len: 206aa; Contains possible membrane spanning hydrophobic regions. 2 4 11 8 [Reporter]SCO0252 (14B21)_PCR/6778 [Gene]SCO0252/6177 NC_003888 SCJ9A.31c, possible oxidoreductase, len: 415 aa; similar to many e.g. TR:AAD25066 (EMBL:AF121000) tetracycline resistance associated oxidoreductase homolog from Corynebacterium glutamicum (402 aa) fasta scores; opt: 672, z-score: 786.6, E(): 0, (32.8% identity in 390 aa overlap) and SW:TETX_BACFR tetracycline resistance oxidoreductase from Bacteroides fragilis transposons (388 aa) fasta scores; opt: 275, z-score: 324.5, E(): 1e-10, (24.9% identity in 377 aa overlap). Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase. 2 4 11 7 [Reporter]SCO3715 (15N17)_PCR/6777 [Gene]SCO3715/6176 NC_003888 SCH35.09, possible ECF sigma factor, len: 223aa; similar to many egs. TR:O53378 (EMBL:AL021841) putative sigma factor from Mycobacterium tuberculosis (312 aa) fasta scores; opt: 357, z-score: 418.3, E(): 5.7e-16, (33.2% identity in 226 aa overlap) and SW:RPOE_STRCO sigma factor E from Streptomyces coelicolor (176 aa) fasta scores; opt: 131, z-score: 162.5, E(): 0.1, (33.3% identity in 114 aa overlap). NB. Overlaps with downstream CDS. 2 4 11 6 [Reporter]SCO3631 (16J17)_PCR/6776 [Gene]SCO3631/6175 NC_003888 SCH10.09, hypothetical protein, len: 169 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 2 4 11 5 [Reporter]SCO3369 (17F17)_PCR/6775 [Gene]SCO3369/6174 NC_003888 SCE94.20, possible large ATP-binding protein, len: 862aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). Similar to two closely neighbouring genes SCE94.23 and SCE94.21 suggesting local duplication fasta scores; SCE94.23 opt: 1840, z-score: 1675.4, E(): 0, (48.9% identity in 839 aa overlap), SCE94.21 opt: 789, z-score: 717.7, E(): 5e-35, (44.7% identity in 877 aa overlap). 2 4 11 4 [Reporter]SCO1048 (18B17)_PCR/6774 [Gene]SCO1048/6173 NC_003888 SCG20A.28c, possible secreted protein, len: 150 aa; similar to TR:Q9Z4W (EMBL:AL035654) Streptomyces coelicolor hypothetical 16.4 kD protein SCE8.15c, 151 aa; fasta scores: opt: 727 z-score: 866.9 E(): 0; 72.1% identity in 147 aa overlap 2 4 11 3 [Reporter]SCP1.291c (19N13)_PCR/6773 [Gene]SCP1.291c/6172 NC_003888 SCP1.291c, unknown, len: 137aa; 2 4 11 2 [Reporter]SCO3989 (20F13)_PCR/6772 [Gene]SCO3989/6171 NC_003888 SCBAC25E3.26, possible ribonuclease inhibitor, len: 89 aa: similar to SW:P11540 (BARS_BACAM) barstar (ribonuclease inhibitor) from Bacillus amyloliquefaciens (89 aa) fasta scores; opt: 141, Z-score: 198.8, 30.667% identity (30.667% ungapped) in 75 aa overlap. Contains Pfam match to entry PF01337 Barstar, Barstar (barnase inhibitor). 2 4 10 22 [Reporter]SCO5267 (1F9)_PCR/6770 [Gene]SCO5267/6170 NC_003888 2SC7G11.29c, unknown, len: 61 aa. High content in glutamic acid amino acid residues 2 4 10 21 [Reporter]SCO5848 (2B9)_PCR/6769 [Gene]SCO5848/6169 NC_003888 SC9B10.15, agaZ, possible tagatose 6-phosphate kinase, len: 435; highly similar to AGAZ_ECOLI P42903 putative tagatose 6-phosphate kinase (426 aa), fasta scores; opt: 1637 z-score: 2100.1 E(): 0, 57.5% identity in 421 aa overlap 2 4 10 20 [Reporter]SCO5359 (3N5)_PCR/6768 [Gene]SCO5359/6168 NC_003888 2SC6G5.03, rpmE3, 50S ribosomal protein L31, len: 74 aa; strongly similar to many eg. SW:P02432 (RL31_ECOLI) 50S ribosomal protein L31 from Escherichia coli (70 aa) fasta scores; opt: 252, z-score: 365.9, E(): 6.6e-13, 50.7% identity in 73 aa overlap. Contains Pfam match to entry PF01197 Ribosomal_L31, Ribosomal protein L31 and Prosite match to PS01143 Ribosomal protein L31 signature. 2 4 10 19 [Reporter]SCO6615 (4J5)_PCR/6767 [Gene]SCO6615/6167 NC_003888 SC1F2.12. unknown, len: 400 aa 2 4 10 18 [Reporter]SCO5716 (5F5)_PCR/6766 [Gene]SCO5716/6166 NC_003888 SC3C3.02c, probable peptide transport system secreted peptide binding protein, len: 621 aa; similar to e.g. DPPA_ECOLI periplasmic dipeptide transport protein precursor (535 aa), fasta scores; opt: 195 z-score: 391.5 E(): 1.5e-14, 26.4% identity in 550 aa overlap. Contains N-terminals signal sequence, possible membrane anchor at C-terminus and Pfam match to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5, score 48.60, E-value 1.1e-11 2 4 10 17 [Reporter]SCO6651 (6B5)_PCR/6765 [Gene]SCO6651/6165 NC_003888 SC5A7.01, possible glycosyl transferase, partial CDS, len: >368 aa; similar to several hypothetical proteins e.g. YV12_MYCTU MTCY20G9.12 (480 aa), fasta sores; opt: 237 z-score: 252.3 E(): 8.1e-07, 29.9% identity in 278 aa overlap, and to WCAL_ECOLI putative colanic acid biosynthesis glycosyl transferase (406 aa), fasta sores; opt: 187 z-score: 194.8 E(): 0.0013, 27.8% identity in 237 aa overlap. Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1, score 52.90, E-value 5.5e-14,SC4G2.25, possible glycosyl transferase, partial CDS, len: >78 aa; overlaps and extends putative glycosyl transferase SC5A7.01 2 4 10 16 [Reporter]SCO3599 (7N1)_PCR/6764 [Gene]SCO3599/6164 NC_003888 SC66T3.10c, hypothetical protein, len: 318 aa; unknown function, similar to hypothetical proteins e.g. TR:O54025 (EMBL:AJ000884), ORF2, Paracoccus denitrificans hypothetical protein (281 aa), fasta scores; opt: 244 z-score: 272.8 E(): 7.2e-08, 31.5% identity in 295 aa overlap 2 4 10 15 [Reporter]SCO5559 (8J1)_PCR/6763 [Gene]SCO5559/6163 NC_003888 SC7A1.03, gpsA, glycerol-3-phosphate dehydrogenase, len: 366aa; similar to many eg. SW:GPDA_BACSU glycerol-3-phosphate dehydrogenase from Bacillus subtilis (345 aa) fasta scores; opt: 874, z-score: 1221.4, E(): 0, (41.8% identity in 330 aa overlap). Contains a possible PS00017 ATP /GTP-binding site motif A (P-loop) and Pfam match to entry PF01210 NAD_Gly3P_dh, NAD-dependent glycerol-3-phosphate dehydrogenase, score 295.10, E-value 8.8e-85. 2 4 10 14 [Reporter]SCO6600 (9F1)_PCR/6762 [Gene]SCO6600/6162 NC_003888 SC8A6.21c, probable transcriptional regulator, len: 441 aa; some similarity to members of the ROK family e.g. XYLR_ANATH xylose repressor (399 aa), fasta scores; opt: 365 z-score: 361.7 E(): 6.6e-13, 27.2% identity in 397 aa over lap. Contains Pfam match to entry PF00480 ROK, ROK family, score 38.10, E-value 3e-10 2 4 10 13 [Reporter]SCO6475 (10B1)_PCR/6761 [Gene]SCO6475/6161 NC_003888 SC9C7.11c, probable oxidoreductase, len: 601aa; similar to many eg. TR:Q45815 (EMBL:M31799) NAD-dependent beta-hydroxybutyryl coenzyme dehydrogenase from Clostridium acetobutylicum (282 aa) fasta scores; opt: 801, z-score: 936.4, E(): 0, (41.1% identity in 280 aa overlap). Appears to be a fusion of two dehydrogesase as each half contains Pfam match to entry PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase. 2 4 10 12 [Reporter]SCO2755 (10N21)_PCR/6760 [Gene]SCO2755/6160 NC_003888 SCC57A.26c, possible acetyltransferase, len: 220 aa. Weakly similar to Deinococcus radiodurans TR:AAF10183 (EMBL:AE001918) putative acetyltransferase (188 aa), fasta scores opt: 308 z-score: 385.7 E(): 4.3e-14 37.0% identity in 189 aa overlap and Myxococcus xanthus TR:O54394 (EMBL:U81516) hypothetical 24.4 KD protein (216 aa), fasta scores opt: 453 z-score: 561.2 E(): 7.2e-24 46.6% identity in 206 aa overlap. Contains a Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 2 4 10 11 [Reporter]SCO4668 (11J21)_PCR/6759 [Gene]SCO4668/6159 NC_003888 SCD40A.14, possible two-component system response regulator, len: 210 aa; similar to TR:CAB61727 (EMBL:AL133220) Streptomyces coelicolor SCC75A.27c, 228 aa; fasta scores: opt: 732 z-score: 839.5 E(): 0; 58.6% identity in 210 aa overlap. Contains two Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family. Contains also possible helix-turn-helix motif at residues 162..183 (+3.44 SD) 2 4 10 10 [Reporter]SCO4315 (12F21)_PCR/6758 [Gene]SCO4315/6158 NC_003888 SCD95A.48, possible homeostasis protein, len: 229 aa; similar to SW:CUTC_ECOLI (EMBL:L38618) Escherichia coli copper homeostasis protein CutC, 248 aa; fasta scores: opt: 302 z-score: 336.1 E(): 2.9e-11; 31.3% identity in 208 aa overlap 2 4 10 9 [Reporter]SCO5167 (13B21)_PCR/6757 [Gene]SCO5167/6157 NC_003888 SCP8.30, conserved hypothetical protein, len: 260 aa; similar to TR:O05856 (EMBL:Z95120) Mycobacterium tuberculosis hypothetical 25.1 kD protein MTCY07D11.16, 231 aa; fasta scores: opt: 599 z-score: 647.2 E(): 1.4e-28; 42.5% identity in 233 aa overlap. Contains 4x degenerate repeat:T(G/A)G 2 4 10 8 [Reporter]SCO2897 (14N17)_PCR/6756 [Gene]SCO2897/6156 NC_003888 SCE6.34, probable secreted penicillin-binding protein, len: 756 aa. Similar to many including: Bacillus subtilis SW:PBPD_BACSU(EMBL:U11882) penicillin-binding protein 4 precursor (624 aa), fasta scores opt: 550 z-score: 456.8 E(): 5.5e-18 28.1% identity in 572 aa overlap and Helicobacter pylori TR:O25319(EMBL:AE000573) penicillin-binding protein 1A (659 aa), fasta scores opt: 750 z-score: 619.0 E(): 5.1e-27 31.9% identity in 605 aa overlap. Contains Pfam matches to entries PF00912 Transglycosyl, Transglycosylase and PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain as well a possible N-terminal signal sequence. 2 4 10 7 [Reporter]SCO1655 (15J17)_PCR/6755 [Gene]SCO1655/6155 NC_003888 SCI41.38c, probable lipoprotein oligopeptide binding protein, len: 534 aa. Similar to TR:O54107 (EMBL:AL021529) Streptomyces coelicolor SC10A5.16C periplasmic oligopeptide binding protein, 521 aa; fasta scores: opt: 1101 z-score: 1218.9 E(): 0; 36.0% identity in 528 aa overlap. Contains two Pfam matches to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5, N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 2 4 10 6 [Reporter]SCO3690 (16F17)_PCR/6754 [Gene]SCO3690/6154 NC_003888 SCH35.34c, possible hydrolase, len: 269 aa; similar to TR: O07015 (EMBL:Z94043) hypothetical protein from Bacillus subtilis (269 aa) fasta scores; opt: 907, z-score: 1096.7, E(): 0, (47.7% identity in 262 aa overlap). Also weakly similar to TR:BAA34263 (EMBL:AB014348) 2-hydroxy-6-oxo-6-phenylehexa-2,4-dienoate hydrolase from Rhodococcus erythropolis TA421 (297 aa) fasta scores; opt: 227, z-score: 278.9, E(): 3.3e-08, (27.1% identity in 277 aa overlap). Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 2 4 10 5 [Reporter]SCO5166 (17B17)_PCR/6753 [Gene]SCO5166/6153 NC_003888 SCP8.29c, possible helicase, len: 879 aa; N-terminal domain similar to SW:RHLE_ECOLI (EMBL:L02123) Escherichia coli putative ATP-dependent RNA helicase RhlE, 454 aa; fasta scores: opt: 884 z-score: 704.8 E(): 8.6e-32; 37.2% identity in 444 aa overlap. Contains Pfam matches to entries PF00270 DEAD, DEAD/DEAH box helicase and PF00271 helicase_C, Helicases conserved C-terminal domain and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00039 DEAD-box subfamily ATP-dependent helicases signature. Also contains three sets of different repeats: 10x RU1: RRTRK, 4x RU2:AAAKAEAAA and 4x RU3:AAEPA 2 4 10 4 [Reporter]SCO6078 (18N13)_PCR/6752 [Gene]SCO6078/6152 NC_003888 SCBAC1A6.02c, probable alpha amylase, len: 581 aa; highly similar to TR:Q44316 (EMBL:D63343) Arthrobacter sp. maltooligosyl trehalose trehalohydrolase TreZ, 598 aa; fasta scores: opt: 2063 z-score: 2298.1 E(): 0; 53.3% identity in 583 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase 2 4 10 3 [Reporter]SCP1.119 (19J13)_PCR/6751 [Gene]SCP1.119/6151 NC_003888 SCP1.119, dnaN, probable DNA-polymerase III, beta chain, len: 372aa; previously sequeneced (with discrepencies) as TR:AAF68381 (EMBL:AF235031) putative DNA-polymerase III, beta chain (366 aa) fasta scores; opt: 1984, z-score: 2263.4, E(): 0, 90.5% identity in 367 aa overlap. Similar to many others eg. SW:P27903 (DP3B_STRCO) DnaN, DNA-polymerase III, beta chain from Streptomyces coelicolor (376 aa) fasta scores; opt: 1054, z-score: 1205.0, E(): 0, 47.6% identity in 380 aa overlap. Contains Pfam match to entry PF00712 DNA_pol3_beta, DNA polymerase III beta subunit. 2 4 10 2 [Reporter]SCO7486 (20B13)_PCR/6750 [Gene]SCO7486/6150 NC_003888 SCBAC17A6.19c, possible ROK-family transcriptional regulator, len: 405 aa; similar to many e.g. TR:P95610 (EMBL:X99622) putative regulator from Rhodococcus erythropolis (397 aa) fasta scores: opt: 548, Z-score: 588.4, 36.776% identity (38.220% ungapped) in 397 aa overlap. Contains Pfam match to entry PF00480 ROK, ROK family and Prosite match to PS01125 ROK family signature. 2 4 9 22 [Reporter]SCO5263 (1B9)_PCR/6748 [Gene]SCO5263/6149 NC_003888 2SC7G11.25, unknown, len: 188 aa 2 4 9 21 [Reporter]SCO6408 (2N5)_PCR/6747 [Gene]SCO6408/6148 NC_003888 SC3C8.27c, clpA, probable clp protease ATP binding subunit, len: 842 aa; highly similar to many e.g. CLPB_ECOLI clpB protein (857 aa), fasta scores; opt: 975 z-score: 1914.6 E(): 0, 46.8% identity in 857 aa overlap anCLAB_LYCES ATP-dependent clp protease atp-binding subunit (923 aa), fasta scores; opt: 2335 z-score: 2174.3 E(): 0, 50.3% identity in 758 aa overlap. Contains 2x PS00017 ATP/GTP-binding site motif A (P-loop), PS00870 and PS00871 Chaperonins clpA/B signatures 1 and 2, Pfam match to entry clpA_B PF00495, Chaperonins clpA/B, score 1127.99 and probable coiled-coil from aa 432 to 488 2 4 9 20 [Reporter]SCO4713 (3J5)_PCR/6746 [Gene]SCO4713/6147 NC_003888 SCD31.38, rplX, 50S ribosomal protein L24, len: 107 aa; highly similar to SW:RL24_BACSU (EMBL:X15664) Bacillus subtilis 50S ribosomal protein L24 RplX, 103 aa; fasta scores: opt: 390 z-score: 475.1 E(): 4.9e-19; 62.9% identity in 105 aa overlap. Contains Pfam match to entry PF00467 Ribosomal_L24, KOW motif and match to Prosite entry PS01108 Ribosomal protein L24 signature 2 4 9 19 [Reporter]SCO6924 (4F5)_PCR/6745 [Gene]SCO6924/6146 NC_003888 SC1B2.30c, possible DNA-binding protein, len: 298 aa. Contains a helix-turn-helix motif situated between residues 66..87 (+2.59 SD). 2 4 9 18 [Reporter]SCO6364 (5B5)_PCR/6744 [Gene]SCO6364/6145 NC_003888 SC3A7.32c, probable two-component regulator, len: 263 aa; similar to many e.g. PHOP_BACSU alkaline phosphatase synthesis transcriptional regulator (240 aa), fasta scores; opt: 452 z-score: 542.7 E(): 5.6e-23, 34.2% identity in 237 aa overlap. Contains Pfam matches to entry PF00072 response_reg, Response regulator receiver domain, score 107.60, E-value 2.4e-28 and to entry PF00486 trans_reg_C, Transcriptional regulatory proteins, C terminal, score 58.80, E-value 3.3e-16 2 4 9 17 [Reporter]SCO5771 (6N1)_PCR/6743 [Gene]SCO5771/6144 NC_003888 SC4H8.10c, hypothetical protein, len: 132 aa; similar to TR:AAK45350 (EMBL:AE006990) Mycobacterium tuberculosis CDC1551 conserved hypothetical protein MT1096, 131 aa; fasta scores: opt: 429 Z-score: 543.2 E(): 1.3e-22; 57.143% identity in 119 aa overlap 2 4 9 16 [Reporter]SCO3595 (7J1)_PCR/6742 [Gene]SCO3595/6143 NC_003888 SC66T3.06, ddlA2, probable D-alanine:D-lactate ligase, len: 346 aa; highly similar to many e.g. TR:O33806 (EMBL:U82965) Streptomyces toyocaensis D-alanine:D-alanine ligase related protein (340 aa), fasta scores; opt: 1831 z-score: 2178.0 E(): 0, 78.4% identity in 338 aa overlap. Also similar to D-alanine:D-lactate ligases from vancomycin-resistant bacteria e.g. SW:VANB_ENTFC (EMBL:U00456), vanB, Enterococcus faecalis D-alanine:D-lactate ligase (342 aa) (63.0% identity in 341 aa overlap). Similar to SC7A1.04, DdlA, (EMBL:AL034447) S.coelicolor putative D-alanine-D-alanine ligase (389 aa) (38.6% identity in 368 aa overlap). Contains PS00844 D-alanine--D-alanine ligase signature 2 2 4 9 15 [Reporter]SCO2145 (8F1)_PCR/6741 [Gene]SCO2145/6142 NC_003888 SC6G10.18, unknown, len: 432aa; similar to many hypothetical proteins eg. SW:YXAA_BACSU hypothetical protein from Bacillus subtilis (382 aa) fasta scores; opt: 1005, z-score: 1049.9, E(): 0, (44.1% identity in 381 aa overlap). Note predicted C-terminal overlap with downstream CDS. Simialr also to SW:GRK_BACSU (EMBL:AB005554) Bacillus subtilis glycerate kinase (EC 2.7.1.31) GlxK or S14A, 382 aa; fasta scores: opt: 1005 Z-score:1015.2 E(): 6.5e-49; 44.094% identity in 381 aa overlap 2 4 9 14 [Reporter]SCO6596 (9B1)_PCR/6740 [Gene]SCO6596/6141 NC_003888 SC8A6.17, probable secreted protein, len: 589 aa; co ntains N-terminal signal sequence and PS00213 Lipocalin sig nature 2 4 9 13 [Reporter]SCO1478 (9N21)_PCR/6739 [Gene]SCO1478/6140 NC_003888 SC9C5.02c, putative DNA-directed RNA polymerase omega chain, len: 90 aa; similar to SW:RPOZ_MYCTU (EMBL:Z80108) Mycobacterium tuberculosis probable DNA-directed RNA polymerase omega chain 11.8 kD protein MTCY21B4.07, 110 aa; fasta scores: opt: 363 z-score: 502.9 E(): 1.5e-20; 69.8% identity in 86 aa overlap 2 4 9 12 [Reporter]SCO2751 (10J21)_PCR/6738 [Gene]SCO2751/6139 NC_003888 SCC57A.22c, unknown, len: 388 aa. 2 4 9 11 [Reporter]SCO4077 (11F21)_PCR/6737 [Gene]SCO4077/6138 NC_003888 SCD25.13, unknown, len: 90 aa. Highly similar to several hypotheticals e.g. Mycobacterium leprae SW: Y524_MYCLE (EMBL; Z95151) hypothetical 8.6 KD protein MLCB5.24 (79 aa), fasta scores opt: 329 z-score: 432.7 E(): 9.5e-17 68.3% identity in 82 aa overlap. 2 4 9 10 [Reporter]SCO4311 (12B21)_PCR/6736 [Gene]SCO4311/6137 NC_003888 SCD95A.44c, hypothetical protein, len: 365 aa; similar to TR:O31648 (EMBL:Z99110) Bacillus subtilis YjdG protein, 168 aa; fasta scores: opt: 214 z-score: 251.8 E(): 1.5e-06; 30.5% identity in 154 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 2 4 9 9 [Reporter]SCO0960 (13N17)_PCR/6735 [Gene]SCO0960/6136 NC_003888 SCM11.15c, possible hydrolase (putative secreted protein), len: 872 aa; similar to TR:Q9X0F2 (EMBL:AE001766) Thermotoga maritima beta-glucuronidase TM1062, 563 aa; fasta scores: opt: 420 z-score: 447.6 E(): 1.5e-17; 28.3% identity in 406 aa overlap and to SW:BGLR_ECOLI (EMBL:M14641) Escherichia coli beta-glucuronidase (EC 3.2.1.31) UidA or GusA or GurA, 603 aa; fasta scores: opt: 359 z-score: 382.2 E(): 6.6e-14; 26.2% identity in 454 aa overlap. Contains possible N-terminal region signal peptide sequence 2 4 9 8 [Reporter]SCO2993 (14J17)_PCR/6734 [Gene]SCO2993/6135 NC_003888 SCE50.21, unknown, len: 184 aa 2 4 9 7 [Reporter]SCO3923 (15F17)_PCR/6733 [Gene]SCO3923/6134 NC_003888 SCQ11.06, hypothetical protein, len: 105 aa; previously sequenced as SW:YPT2_STRCO (EMBL:U37580) hypothetical 8.2 kD protein in PtpA 5' region (77 aa), with different putative start codon. Start codon position is suggested here by GC frameplot analysis. Similar to others e.g. to part of TR:O06336 (EMBL:Z95390) Mycobacterium tuberculosis hypothetical protein (177 aa), fasta scores; opt: 145 z-score: 207.2 E(): 0.00033, (42.1% identity in 57 aa overlap) and to TR:Q59013 (EMBL:U67602) Methanococcus jannaschii hypothetical protein (125 aa) (30.2% identity in 63 aa overlap). Weakly similar to SC9B5.02 (EMBL:AL035206) S.coelicolor hypothetical protein (128 aa) (28.9% identity in 76 aa overlap) 2 4 9 6 [Reporter]SCO5931 (16B17)_PCR/6732 [Gene]SCO5931/6133 NC_003888 SC10A5.36c, xlnA, xylanase A precursor, partial CDS, len >383 aa; overlaps and extends ORF from neighbouring cosmid SC7H1.01c; almost identical to XYNA_STRLI P26514 endo- 1,4-beta-xylanase a precursor (477 aa) (99.2% identity in 383 aa overlap). Conatains PS00591 Glycosyl hydrolases famil y 10 active site and Pfam match to entry glycosyl_hydro3 PF 00331, Glycosyl hydrolases family 10, score 183.41. Also si milar in part to S. coelicolor SC7H1.02, abfB, arabinofuran osidase (E(): 1.4e-35, 58.4% identity in 149 aa overlap),SC7H1.01c, xlnA, xylanase A precursor, partial CDS, len >147 aa; almost identical to S. lividans XYNA_STRLI P26514 endo-1,4-beta-xylanase a precursor (ec 3.2.1.8) (XYLANASE A) (477 aa), fasta scores; opt: 926 z-score: 1591.1 E(): 0, 97.9% identity in 146 aa overlap. Contains N-terminal signal sequence and Pfam match to entry glycosyl_hydro3 PF00331, Glycosyl hydrolases family 10, score 107.60 2 4 9 5 [Reporter]SCO3316 (17N13)_PCR/6731 [Gene]SCO3316/6132 NC_003888 SCE68.14c, probable dehydrogenase, len: 258 aa; similar to many e.g. SW:DHG3_BACME (EMBL:D10625), gdhIII, Bacillus megaterium glucose 1-dehydrogenase III (261 aa), fasta scores; opt: 424 z-score: 446.0 E(): 1.6e-17,32.4% identity in 253 aa overlap. Similar to many others from S.coelicolor e.g. TR:O88068 (EMBL:AL031541), SCI35.33c, probable dehydrogenase (260 aa) (33.5% identity in 254 aa overlap). Contains Pfam matches to entry PF00106 adh_short, short chain dehydrogenase and entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus. Contains PS00061 Short-chain dehydrogenases/reductases family signature 2 4 9 4 [Reporter]SCP1.263c (18J13)_PCR/6730 [Gene]SCP1.263c/6131 NC_003888 SCP1.263c, possible membrane protein, len: 103aa; contains possible membrane-spanning hydrophobic region. 2 4 9 3 [Reporter]SCO6279 (19F13)_PCR/6729 [Gene]SCO6279/6130 NC_003888 SC1G7.05, probable diaminobutyrate-pyruvate aminotransferase, len: 518 aa; similar to SW:RHSA_RHIME (EMBL:AF110737) Rhizobium meliloti diaminobutyrate-pyruvate aminotransferase (EC 2.6.1.76) RhsA, 470 aa; fasta scores: opt: 423 Z-score: 464.1 bits: 95.4 E(): 2.9e-18; 30.704% identity in 469 aa overlap. Contains 2x Pfam matches to entry PF00202 aminotran_3, Aminotransferase class-III pyridoxal-phosphate 2 4 9 2 [Reporter]SCO0584 (20N9)_PCR/6728 [Gene]SCO0584/6129 NC_003888 SCF55.08c, possible cytochrome P450, len: 420 aa; similar to TR:O87605 (EMBL:AF087022) Streptomyces violaceus (Streptomyces venezuelae) cytochrome P450 monooxygenase PicK, 416 aa; fasta scores: opt: 494 z-score: 582.0 E(): 4.8e-25; 31.3% identity in 416 aa overlap, to SW:CPXF_STRGO (EMBL:M32239) Sterptomyces griseolus cytochrome P450-SU2 (EC 1.14.-.-) (P450-CVB1) (CYP105B1), SubC, 402 aa; fasta scores: opt: 405 z-score: 477.7 E(): 3.1e-19; 29.6% identity in 412 aa overlap and to Streptomyces coelicolor SCF55.07, 407 aa; fasta scores: opt: 204 z-score: 217.0 E(): 9.5e-07; 27.4% identity in 416 aa overlap 2 4 8 22 [Reporter]SCO4208 (1N5)_PCR/6726 [Gene]SCO4208/6128 NC_003888 2SCD46.22c, possible integral membrane transport protein, len: 429aa; weakly similar to many membrane proteins eg. TR:O53854 (EMBL:AL022004) putative membrane protein from Mycobacterium tuberculosis (430 aa) fasta scores; opt: 382, z-score: 428.1, E(): 2.3e-16, 26.1% identity in 399 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and multiple possible membrane-spanning hydrophobic regions. 2 4 8 21 [Reporter]SCO1429 (2J5)_PCR/6725 [Gene]SCO1429/6127 NC_003888 SC6D7.10, chiD, chitinase (putative secreted protein), len: 417 aa. Previously sequenced and characterised TR:Q9Z9M7(EMBL:AB017011) fasta scores opt: 2877 z-score: 3125.2 E():0 100.0% identity in 417 aa overlap. Contains a Pfam match to entry PF00704 Glyco_hydro_18, Glycosyl hydrolases family 18 and a Prosite hit to PS01095 Chitinases family 18 active site. Contains possible N-terminal region signal peptide sequence 2 4 8 19 [Reporter]SCO6920 (4B5)_PCR/6723 [Gene]SCO6920/6126 NC_003888 SC1B2.26c, doubtful CDS, len: 110 aa. 2 4 8 18 [Reporter]SCO6840 (5N1)_PCR/6722 [Gene]SCO6840/6125 NC_003888 SC3D9.08c, unknown, len: 681 aa. 2 4 8 17 [Reporter]SCO5765 (6J1)_PCR/6721 [Gene]SCO5765/6124 NC_003888 SC4H8.04c, unknown, len: 341 aa; first start site used 2 4 8 16 [Reporter]SCO0298 (7F1)_PCR/6720 [Gene]SCO0298/6123 NC_003888 SC5G9.07c, probable lysR-family transcriptional regulator, len: 287 aa; similar to many e.g. SW:ALSR_BACSU (EMBL:L04470), AlsR, Bacillus subtilis regulator of acetoin biosynthesis operon (302 aa), fasta scores; opt: 424 z-score: 491.8 E(): 5e-20, 31.6% identity in 253 aa overlap. Contains probable helix-turn-helix motif at aa 20-41 (Score 1533, +4.41 SD). Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and PS00044 Bacterial regulatory proteins, lysR family signature 2 4 8 15 [Reporter]SCO2141 (8B1)_PCR/6719 [Gene]SCO2141/6122 NC_003888 SC6G10.14, possible small secreted hydrophilic protein, len: 107aa; contains possible N-terminal signal sequence. 2 4 8 14 [Reporter]SCO7189 (8N21)_PCR/6718 [Gene]SCO7189/6121 NC_003888 SC8A11.17c, hypothetical protein, len: 87 aa; similar to SW:Y898_MYCTU (EMBL:Z73101) Mycobacterium tuberculosis hypothetical 9.9 kDa protein MTCY31.26c, 87 aa; fasta scores: opt: 256 z-score: 337.1 E(): 2.6e-11; 48.2% identity in 85 aa overlap. Highly hydrophilic 2 4 8 13 [Reporter]SCO6461 (9J21)_PCR/6717 [Gene]SCO6461/6120 NC_003888 SC9B5.28, possible ADA-like regulatory protein, len: 490 aa; similar to SC1A9.14 (EMBL:AL034446) possible ADA-like regulatory protein from Streptomyces coelicolor (477 aa) fasta scores: opt: 1192, z-score: 937.1, E(): 0, (53.3% identity in 501 aa overlap). Also similar to ADA_MYCTU putative ADA regulatory protein from Mycobacterium tuberculosis (496 aa) fasta scores; opt: 2185, z-score: 2395.4, E(): 0, (66.7% identity in 490 aa overlap). Also note N-terminal region similar to N-terminal region of ADA_ECOLI ADA regulatory protein from Escherichia coli (354 aa) fasta scores; opt: 257, z-score: 248.0, E(): 1.6e-06, (31.3% identity in 182 aa overlap) and C-terminal region similar to 3MG2_ECOLI AlkA, 3-methyladenine DNA glycosylase II involved in DNA repair (282 aa) fasta scores; opt: 450, z-score: 413.0, E(): 1e-15, (32.5% identity in 286 aa overlap). Contains PS00041 araC family signature, Pfam matches PF00165 HTH_2 helix-turn-helix /araC and PF01332 AlkA_DNA_repair, Alkylbase DNA glycosidases alkA family. 2 4 8 12 [Reporter]SCO2423 (10F21)_PCR/6716 [Gene]SCO2423/6119 NC_003888 SCC42.04, possible secreted protein, len: 210 aa. Contains possible N-terminal region signal peptide sequence 2 4 8 11 [Reporter]SCO4073 (11B21)_PCR/6715 [Gene]SCO4073/6118 NC_003888 SCD25.09c, possible sensor kinase, len: 415 aa. Identical to Streptomyces lividans TR:Q04390 (EMBL:X65556) hypothetical protein in tRNA-glyU beta gene region (fragment) (>64 aa), fasta scores opt: 436 z-score: 463.7 E(): 1.8e-18 100.0% identity in 64 aa overlap. Also similar to Streptomyces coelicolor TR:O70000 (EMBL:AL022374) putative two-component sensor SC5B8.14 (384 aa), fasta scores opt: 303 z-score: 317.3 E(): 2.5e-10 26.8% identity in 400 aa overlap. Contains possible membrane spanning hydrophobic domains 2 4 8 10 [Reporter]SCO2913 (12N17)_PCR/6714 [Gene]SCO2913/6117 NC_003888 SCE19A.13c, conserved hypothetical protein, len: 140 aa; unknown function, similar to hypothetical proteins from other organisms e.g. SW:Y03J_MYCTU (EMBL:Z73902) Mycobacterium tuberculosis hypothetical protein (146 aa), fasta scores; opt: 579 z-score: 694.1 E(): 2.5e-31, 65.0% identity in 140 aa overlap and TR:Q54330 (EMBL:M29166) Streptomyces kasugaensis CDS from Mec+ region (115 aa) (69.8% identity in 116 aa overlap) 2 4 8 9 [Reporter]SCO3461 (13J17)_PCR/6713 [Gene]SCO3461/6116 NC_003888 SCE46.18, possible integral membrane protein, len: 152 aa; similar to SW: MAUE_PARDE (EMBL:X98581) Paracoccus denitrificans methylamine utilization protein MauE, 186 aa; fasta scores: opt: 162 z-score: 208.6 E(): 0.00028; 28.1% identity in 139 aa overlap. Contains possible N-terminal region signal peptide sequence 2 4 8 8 [Reporter]SCO1809 (14F17)_PCR/6712 [Gene]SCO1809/6115 NC_003888 SCI28.03c, unknown, len: 172aa; similar to SW:Y086_MYCTU hypothetical protein from Mycobacterium tuberculosis (158 aa) fasta scores; opt: 385, z-score: 471.9, E(): 5.9e-19, (43.5% identity in 161 aa overlap). 2 4 8 7 [Reporter]SCO0104 (15B17)_PCR/6711 [Gene]SCO0104/6114 NC_003888 SCJ11.33, possible hydrolase, len: 223aa; similar to TR:O54126 (EMBL:AL021529) putative NTP pyrophosphohydrolase from Streptomyces coelicolor (359 aa) fasta scores; opt: 223, z-score: 267.6, E(): 1.5e-07, (29.4% identity in 194 aa overlap). 2 4 8 6 [Reporter]SCO1034 (16N13)_PCR/6710 [Gene]SCO1034/6113 NC_003888 SCG20A.14, possible tetR-family regulatory protein, len: 217 aa; similar to TR:Q9RJ10 (EMBL:AL109962) Streptomyces coelicolor putative transcriptional regulatory protein SCJ1.04, 207 aa; fasta scores: opt: 208 z-score: 253.6 E(): 1.2e-06; 27.2% identity in 195 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 2 4 8 5 [Reporter]SCO3482 (17J13)_PCR/6709 [Gene]SCO3482/6112 NC_003888 SCE65.18c, possible sugar-permease, len: 293 aa; similar to TR:P72399 (EMBL:Y07706) Streptomyces coelicolor putative maltose permease MalG, 302 aa; fasta scores: opt: 491 z-score: 561.6 E(): 6.6e-24; 28.2% identity in 301 aa overlap. Contains match to Pfam entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains possible N-terminal region signal peptide sequence 2 4 8 4 [Reporter]SCO0830 (18F13)_PCR/6708 [Gene]SCO0830/6111 NC_003888 SCF43A.20c, possible penicillin-binding protein, len: 359 aa; weakly similar to penicillin-binding proteins, carboxypeptidases and beta-lactamases e.g. SW:AMPH_ECOLI (EMBL:AE000144), AmpH, Escherichia coli penicillin-binding protein (376 aa), fasta scores; opt: 274 z-score: 305.5 E(): 1.1e-09, 26.5% identity in 343 aa overlap, SW:DAC_STRSQ (EMBL:M26842) Streptomyces sp. D-alanyl-D-alanine carboxypeptidase precursor (406 aa) (23.8% identity in 344 aa overlap) and SW:AMPC_PSEAE (EMBL:X54719), AmpC, Pseudomonas aeruginosa beta-lactamase precursor (397 aa) (27.3% identity in 381 aa overlap). Weakly similar to others from S. coelicolor e.g. TR:O86739 (EMBL:AL031035) S. coelicolor probable secreted peptidase (386 aa) (26.6% identity in 369 aa overlap) and SCH24.34 (EMBL:AL049826) S. coelicolor possible beta-lactamase (461 aa) (31.4% identity in 325 aa overlap) 2 4 8 3 [Reporter]SCO5999 (19B13)_PCR/6707 [Gene]SCO5999/6110 NC_003888 SCBAC1C11.02, sacA, aconitase, len: 904 aa; identical to previously sequenced TR:Q9RNH9 (EMBL:AF180948) Streptomyces coelicolor aconitase (EC 4.2.1.3) SacA, 904 aa. Contains 2x Pfam matches to entry PF00330 aconitase, Aconitase family (aconitate hydratase) and Pfam match to entry PF00694 Aconitase_C, Aconitase C-terminal domain and matches to Prosite entries PS00450 Aconitase family signature 1 and PS01244 Aconitase family signature 2 2 4 8 2 [Reporter]SCO3318 (20J9)_PCR/6706 [Gene]SCO3318/6109 NC_003888 SCE68.16c, hemC, probable porphobilinogen deaminase, len: 319 aa; similar to many e.g. SW:HEM3_BACSU (EMBL:M57676), hemC, Bacillus subtilis porphobilinogen deaminase (314 aa), fasta scores; opt: 721 z-score: 807.5 E(): 0, 39.8% identity in 314 aa overlap. Contains Pfam match to entry PF01379 Porphobil_deam, Porphobilinogen deaminase 2 4 7 22 [Reporter]SCO4204 (1J5)_PCR/6704 [Gene]SCO4204/6108 NC_003888 2SCD46.18, possible glycosyltransferase, len: 496aa; strongly similar to SW:Q11152 (Y486_MYCTU) hypothetical protein from Mycobacterium tuberculosis (480 aa) fasta scores; opt: 932, z-score: 1025.3, E(): 0, 58.2% identity in 431 aa overlap. Also similar to proposed tranferases eg. TR:Q9Z5B7 (EMBL:AL035478) putative transferase from Streptomyces coelicolor (406 aa) fasta scores; opt: 438, z-score: 484.5, E(): 1.7e-19, 36.2% identity in 436 aa overlap. Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1. 2 4 7 21 [Reporter]SCO0785 (2F5)_PCR/6703 [Gene]SCO0785/6107 NC_003888 3SCF60.17c, possible integral membrane protein, len: 227 aa. Contains possible hydrophobic membrane spanning regions 2 4 7 20 [Reporter]SCO6635 (3B5)_PCR/6702 [Gene]SCO6635/6106 NC_003888 SC4G2.09, pglY, len: 1294 aa; 99.8% identical to S. coelicolor TR:Q53942 (EMBL:L37531) bacteriophage (phiC31) resistance gene pglY (1294 aa). Contains PS00017 ATP /GTP-binding site motif A (P-loop) 2 4 7 19 [Reporter]SCO6037 (4N1)_PCR/6701 [Gene]SCO6037/6105 NC_003888 SC1C3.25, possible transmembrane transport protein, len: 410 aa; similar to e.g. Rhizobium meliloti MOSC_RHIMEmembrane protein mosC (407 aa), fasta scores; opt: 290 z-score: 488.8 E(): 5.4e-20, 29.1% identity in 392 aa overlap 2 4 7 18 [Reporter]SCO5467 (5J1)_PCR/6700 [Gene]SCO5467/6104 NC_003888 SC2A11.01, probable muramoyl-pentapeptide carboxypeptidase, partial CDS, len: 189 aa; highly similar to S. albus CBPM_STRAL muramoyl-pentapeptide carboxypeptidase precursor (EC 3.4.17.8) (255 aa), fasta scores; opt: 955 z-score: 1605.0 E(): 0, 71.1% identity in 190 aa overlap,SC3D11.24, possible muramoyl-pentapeptide carboxypeptidase, partial CDS, len: >88aa; similar to the N-terminal region of SW:P00733 (CBPM_STRAL) muramoyl-pentapeptide carboxypeptidase from Streptomyces albus (255 aa) 2 4 7 17 [Reporter]SCO7339 (6F1)_PCR/6699 [Gene]SCO7339/6103 NC_003888 SC410.18c, unknown, len: 278 aa. Partially sequenced previously: Streptomyces coelicolor TR:Q9X946(EMBL:AJ001206) hypothetical 17.2 kd protein (>161 aa). Also highly similar to Streptomyces griseus TR:P95754(EMBL:D89734) SgaA protein, a suppressor of growth disturbance in the presence of high osmolarity and high concentrations of A-factor (264 aa), fasta scores opt: 807 z-score: 948.0 E():0 52.0% identity in 250 aa overlap. 2 4 7 16 [Reporter]SCO0294 (7B1)_PCR/6698 [Gene]SCO0294/6102 NC_003888 SC5G9.03c, probable acetyltransferase, len: 217 aa; similar to many acetyltransferases which inactivate virginiamycin A-like antibiotics by acetylation e.g. SW:SATA_ENTFC (EMBL:L12033), satA, Enterococcus faecium streptogramin A acetyltransferase (209 aa), fasta scores; opt: 632 z-score: 743.9 E(): 0, 43.6% identity in 202 aa overlap. Contains Pfam match to entry PF00132 hexapep, Bacterial transferase hexapeptide (four repeats) 2 4 7 15 [Reporter]SCO6957 (7N21)_PCR/6697 [Gene]SCO6957/6101 NC_003888 SC6F7.10, possible integral membrane protein, len: 199 aa. Similar to Rhodobacter capsulatus TR:O68037(EMBL:AF010496) poly(3-hydroxyalkanoate) polymerase (EC 2.3.1.) (162 aa), fasta scores opt: 216 z-score: 285.2 E(): 1.9e-08 33.9% identity in 165 aa overlap and Deinococcus radiodurans TR:AAF10456(EMBL:AE001941) conserved hypothetical protein (169 aa), fasta scores opt: 182 z-score: 241.6 E(): 5.1e-06 29.7% identity in 175 aa overlap. Contains possible membrane spanning hydrophobic domains. 2 4 7 14 [Reporter]SCO7185 (8J21)_PCR/6696 [Gene]SCO7185/6100 NC_003888 SC8A11.13, probable branched amino acid binding secreted protein, len: 396 aa; similar to SW:LIVK_ECOLI (EMBL:M29378) Escherichia coli leucine-specific binding protein precursor LivK, 369 aa; fasta scores: opt: 233 z-score: 262.9 E(): 3.6e-07; 24.7% identity in 360 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 1 4 10 11 [Reporter]SCO4667 (11I21)_PCR/6255 [Gene]SCO4667/5699 NC_003888 SCD40A.13, possible two-component system sensor kinase, len: 431 aa; C-terminal region similar to TR:Q9ZBS5 (EMBL:AL034446) Streptomyces coelicolor putative sensor kinase SC1A9.27c, 303 aa; fasta scores: opt: 378 z-score: 412.0 E(): 1.6e-15; 31.9% identity in 313 aa overlap. Contains possible N-terminal region hydrophobic membrane spanning regions 1 4 10 10 [Reporter]SCO4314 (12E21)_PCR/6254 [Gene]SCO4314/5698 NC_003888 SCD95A.47, hypothetical protein, len: 227 aa; similar to TR:O53480 (EMBL:AL021899) Mycobacterium tuberculosis hypothetical 23.2 kD protein MTV018.23, 213 aa; fasta scores: opt: 178 z-score: 217.9 E(): 0.00011; 29.0% identity in 200 aa overlap 1 4 10 9 [Reporter]SCO3752 (13A21)_PCR/6253 [Gene]SCO3752/5697 NC_003888 SCH22A.30, possible ABC transporter ATP-binding protein, len: 252 aa; similar to TR:Q9XAA7 (EMBL:AL096837) Streptomyces coelicolor putative ABC transporter ATP-binding protein SCF43A.08, 264 aa; fasta scores: opt: 848 z-score: 944.8 E(): 0; 56.0% identity in 243 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 4 10 8 [Reporter]SCO0915 (14M17)_PCR/6252 [Gene]SCO0915/5696 NC_003888 SCM10.03, unknown, len: 85 aa; similar to TR:O53714 (EMBL:AL021931) Mycobacterium tuberculosis MTV036.14 possible protein transport protein, 71 aa; fasta scores: opt: 96 z-score: 153.6 E(): 0.36; 24.2% identity in 62 aa overlap 1 4 10 7 [Reporter]SCO3852 (15I17)_PCR/6251 [Gene]SCO3852/5695 NC_003888 SCH69.22c, putative membrane protein, len: 69 aa. Contains possible hydrophobic membrane spanning region 1 4 10 6 [Reporter]SCO0259 (16E17)_PCR/6250 [Gene]SCO0259/5694 NC_003888 SCF1.01, partial CDS, probable alcohol dehydrogenase, len: >167aa; similar the C-terminal region of many eg. SW:ADH3_SULSO NAD-dependent alcohol dehydrogenase from Sulfolobus solfataricus (347 aa) fasta scores; opt: 274, z-score: 304.7, E(): 1.3e-09, (31.8% identity in 170 aa overlap). Also similar to C-terminal region of TR:BAA82700 (EMBL:AB017438) from Streptomyces coelicolor (346 aa) fasta scores; opt: 372, z-score: 409.3, E(): 2e-15, (40.7% identity in 167 aa overlap).,SCF20.05, probable alcohol dehydrogenase (zinc-binding), len: >236aa; similar to many eg. alcohol dehydrogenases eg. SW:ADH1_SULSO NAD-dependent alcohol dehydrogenase from Sulfolobus solfataricus (347 aa) fasta scores; opt: 476, z-score: 530.0, E(): 3.7e-22, (35.4% identity in 243 aa overlap). Also similar to TR:BAA82700 (EMBL:AB017438) hypothetical protein from Streptomyces coelicolor (346 aa) fasta scores; opt: 591,, z-score: 655.8, E(): 3.7e-29, (42.8% identity in 243 aa overlap). Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and Prosite match to PS00059 Zinc-containing alcohol dehydrogenases signature. 1 4 10 5 [Reporter]SCO0723 (17A17)_PCR/6249 [Gene]SCO0723/5693 NC_003888 3SC5B7.01, conserved hypothetical protein (fragment), len: >151 aa; similar to TR:Q9SA67 (EMBL:AC006550) Arabidopsis thaliana F10O3 protein, 270 aa; fasta scores: opt: 219 z-score: 240.7 E(): 7.1e-06; 30.4% identity in 125 aa overlap,SCF42.33, unknown, partial CDS, len: > 117 aa. Similar to a protein of undefined function from Schizosaccharomyces pombe (Fission yeast) TR:CAB61463(EMBL:AL133156) (235 aa), fasta scores opt: 241 z-score: 282.8 E(): 2.3e-08 37.0% identity in 119 aa overlap and Escherichia coli SW:YGGC_ECOLI(EMBL:X14436) (237 aa), fasta scores opt: 184 z-score: 219.2 E(): 8.1e-05 36.4% identity in 99 aa overlap. Contains a Prosite Hit to PS00017 ATP/GTP-binding site motif A (P-loop). 1 4 10 4 [Reporter]SCP1.117 (18M13)_PCR/6248 [Gene]SCP1.117/5692 NC_003888 SCP1.117, unknown, len: 251aa; 1 4 10 3 [Reporter]SCO4881 (19I13)_PCR/6247 [Gene]SCO4881/5691 NC_003888 2SCK8.07, possible polysaccharide biosynthesis related protein, len: 312 aa; similar to SW:SPSE_BACSU (EMBL:X73124) Bacillus subtilis spore coat polysaccharide biosynthesis protein SpsE, 373 aa; fasta scores: opt: 473 z-score: 554.3 E(): 2.6e-23; 32.2% identity in 295 aa overlap and to TR:Q9NR45 (EMBL:AF257466) Homo sapiens N-acetylneuraminic acid phosphate synthase, 359 aa; fasta scores: opt: 660 z-score: 771.2 E(): 0; 38.4% identity in 294 aa overlap. Contains matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00639 Eukaryotic thiol (cysteine) proteases histidine active site 1 4 10 2 [Reporter]SCO3987 (20A13)_PCR/6246 [Gene]SCO3987/5690 NC_003888 SCBAC25E3.22, conserved hypothetical protein, len: 101 aa: similar to TR:Q9X8D6 (EMBL:AL049573) hypothetical protein SCE39.13c from Streptomyces coelicolor (99 aa) fasta scores; opt: 441, Z-score: 546.5, 67.742% identity (67.742% ungapped) in 93 aa overlap 1 4 9 22 [Reporter]SCO5262 (1A9)_PCR/6244 [Gene]SCO5262/5689 NC_003888 2SC7G11.24, possible dehydrogenase, len: 321 aa; similar to SW:ADH3_BACST (EMBL:Z27089) Bacillus stearothermophilus alcohol dehydrogenase (EC 1.1.1.1), 339 aa; fasta scores: opt: 303 z-score: 344.3 E(): 1e-11; 28.0% identity in 328 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases 1 4 9 21 [Reporter]SCO2736 (2M5)_PCR/6243 [Gene]SCO2736/5688 NC_003888 SCC57A.07c, citA, citrate synthase, len: 429 aa. Previously sequenced and characterised: Streptomyces coelicolor TR:AAF14286(EMBL:AF181118) citrate synthase (citA). Contains a Prosite hit to PS00480 Citrate synthase signature and a Pfam match to entry PF00285 citrate_synt, Citrate synthase. 1 4 9 20 [Reporter]SCO4704 (3I5)_PCR/6242 [Gene]SCO4704/5687 NC_003888 SCD31.29, rplW, 50S ribosomal protein L23, len: 139 aa; highly similar to SW:RL23_BACSU (EMBL:D50302) Bacillus subtilis 50S ribosomal protein L23 RplW, 95 aa; fasta scores: opt: 241 z-score: 308.8 E(): 9e-10; 41.8% identity in 91 aa overlap. Contains Pfam match to entry PF00276 Ribosomal_L23, Ribosomal protein L23 and match to Prosite entry PS00050 Ribosomal protein L23 signature 1 4 9 19 [Reporter]SCO6923 (4E5)_PCR/6241 [Gene]SCO6923/5686 NC_003888 SC1B2.29c, unknown, len: 238 aa. 1 4 9 18 [Reporter]SCO6362 (5A5)_PCR/6240 [Gene]SCO6362/5685 NC_003888 SC3A7.30, probable two-component sensor, len: 429 aa; similar to many e.g. UHPB_SALTY sensor protein UhpB (500 aa), fasta scores; opt: 238 z-score: 263.3 E(): 2.1e-07, 30.7% identity in 358 aa overlap. Contains six probable hydrophobic transmembrane domains in N-terminus 1 4 9 17 [Reporter]SCO5768 (6M1)_PCR/6239 [Gene]SCO5768/5684 NC_003888 SC4H8.07c, unknown, len: 66 aa; similar to M. tuberculosis hypothetical protein TR:O06782 (EMBL:Z95972) MTCI376.19 (51 aa), fasta scores; opt: 107 z-score: 185.7 E(): 0.0038, 38.6% identity in 44 aa overlap 1 4 9 16 [Reporter]SCO3594 (7I1)_PCR/6238 [Gene]SCO3594/5683 NC_003888 SC66T3.05, dldh, probable D-lactate dehydrogenase, len: 337 aa; highly similar to many e.g. TR:O33805 (EMBL:U8296) Streptomyces toyocaensis D-lactate dehydrogenase (330 aa), fasta scores; opt: 1470 z-score: 1711.8 E(): 0,71.4% identity in 318 aa overlap. Also similar to D-specific alpha-keto acid dehydrogenases from vancomycin-resistant bacteria e.g. SW:VANH_ENTFC (EMBL:M64304), VanH, Enterococcus faecium alpha-keto acid dehydrogenase (322 aa) (61.4% identity in 316 aa overlap). Similar to other S.coelicolor putative dehydrogenases e.g. part of SerA (EMBL:AL035569) S.coelicolor probable D-3-phosphoglycerate dehydrogenase (529 aa) (32.2% identity in 255 aa overlap). Contains Pfam matches to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases, PS00670 D-isomer specific 2-hydroxyacid dehydrogenases signature 2 and PS00671 D-isomer specific 2-hydroxyacid dehydrogenases signature 3 1 4 9 15 [Reporter]SCO2144 (8E1)_PCR/6237 [Gene]SCO2144/5682 NC_003888 SC6G10.17, possible integral membrane transporter, len: 469aa; similar to many eg. SW:CITT_ECOLI CitR, citrate transporter from Escherichia coli (487 aa) fasta scores; opt: 257, z-score: 284.2, E(): 1.6e-08, (23.3% identity in 454 aa overlap). Contains Pfam match to entry PF00939 Na_sulph_symp, Sodium:sulfate symporter transmembrane region, score -165.10, E-value 3.2e-06 and several probable membrane spanning hydrophobic regions. 1 4 9 14 [Reporter]SCO6595 (9A1)_PCR/6236 [Gene]SCO6595/5681 NC_003888 SC8A6.16, probable secreted protein, len: 380 aa; co ntains N-terminal signal sequence and TTA Leu codon, possib le target for action of bldA 1 4 9 13 [Reporter]SCO1477 (9M21)_PCR/6235 [Gene]SCO1477/5680 NC_003888 SC9C5.01c, probable flavoprotein homologue (partial), len: >375 aa; similar to SW:DFP_ECOLI (EMBL:L10328) Escherichia coli DNA/pantothenate metabolism flavoprotein Dfp, 430 aa; fasta scores: opt: 846 z-score: 937.1 E(): 0; 43.8% identity in 347 aa overlap,SCL6.34c, probable flavoprotein homologue (partial), len: >89 aa; highly similar to TR:Q9X4Q1 (EMBL:AF117274) Streptomyces spectabilis flavoprotein homolog Dfp (fragment), 174 aa; fasta scores: opt: 417 z-score: 566.0 E(): 4.1e-24; 76.4% identity in 89 aa overlap and to C-teminal region of SW:DFP_SYNY3 (EMBL:D90910) Synechocystis sp. DNA/pantothenate metabolism flavoprotein homolog Dfp, 402 aa; fasta scores: opt: 199 z-score: 272.3 E(): 9.6e-08; 33.7% identity in 89 aa overlap 1 4 9 12 [Reporter]SCO2750 (10I21)_PCR/6234 [Gene]SCO2750/5679 NC_003888 SCC57A.21c, hypothetical protein, len: 287 aa; similar to TR:CAC41509 (EMBL:AL591782) Rhizobium meliloti (Sinorhizobium meliloti) conserved hypothetical protein SMC04130, 274 aa; fasta scores: opt: 796 Z-score: 889.6 E(): 6.4e-42; 47.191% identity in 267 aa overlap 1 4 9 11 [Reporter]SCO4076 (11E21)_PCR/6233 [Gene]SCO4076/5678 NC_003888 SCD25.12c, putative LSR2-like protein, len: 105 aa. Highly similar to Streptomyces coelicolor TR:CAB40875 (EMBL; AL049628) putative LSR2-like protein SCE94.26C (111 aa), fasta scores opt: 335 z-score: 425.4 E(): 2.4e-16 50.5% identity in 109 aa overlap and Mycobacterium leprae SW: LSR2_MYCLE (EMBL; M67510) LSR2 protein precursor (15 KD antigen) (112 aa), fasta scores opt: 287 z-score: 367.2 E(): 4.2e-13 46.8% identity in 111 aa overlap. 1 4 9 10 [Reporter]SCO4310 (12A21)_PCR/6232 [Gene]SCO4310/5677 NC_003888 SCD95A.43, hypothetical protein, len: 98 aa; highly similar to TR:P96070 (EMBL:U09991) Streptomyces violaceus ISP5230 chloramphenicol resistance protein (CMLV) and chloramphenicol phosphotransferase genes, complete CDS (fragment), 89 aa; fasta scores: opt: 450 z-score: 589.5 E(): 2.3e-25; 74.1% identity in 85 aa overlap 1 4 9 9 [Reporter]SCO3173 (13M17)_PCR/6231 [Gene]SCO3173/5676 NC_003888 SCE87.24, possible merR-family transcriptional regulator, len: 215 aa. Similar to two other putative transcriptional regulators from Streptomyces coelicolor TR:CAB40683 (EMBL:AL049587) SC5F2A.16C (251 aa), fasta scores opt: 210 z-score: 257.9 E(): 5.4e-07 32.3% identity in 217 aa overlap and TR:O86531 (EMBL:AL031124) SC1C2.31C (214 aa), fasta scores opt: 176 z-score: 218.8 E(): 8.2e-05 30.4% identity in 191 aa overlap. Contains a Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family with the putative helix-turn-helix motif situated between residues 8..29 (+3.90 SD). 1 4 9 8 [Reporter]SCO0273 (14I17)_PCR/6230 [Gene]SCO0273/5675 NC_003888 SCF85.01, unknown, partial CDS, len: >264 aa. Weakly similar to several hypotheticals e.g. Mycobacterium tuberculosis TR:O53485 (EMBL; AL021899) lipoprotein component of sugar transport system (439 aa), fasta scores opt: 258 z-score: 307.2 E(): 9.3e-10 27.9% identity in 222 aa overlap and Thermotoga maritima TR:AAD35892 (EMBL; AE001748) sugar ABC transporter, periplasmic sugar-binding protein TM0810 (420 aa), fasta scores opt: 169 z-score: 203.2 E(): 0.00058 25.1% identity in 263 aa overlap.,SCF1.15, partial CDS, possible substrate binding protein (extracellular) (binding-protein-dependent transport), len: >221aa; similar to many the N-terminal region of undefined function eg. SW:YURO_BACSU hypothetical ABC transporter extracellular binding protein from Bacillus subtilis (422 aa) fasta scores; opt: 224, z-score: 276.0, E(): 5.2e-08, (26.1% identity in 161 aa overlap). Contains possible N-terminal signal sequence. 1 4 9 7 [Reporter]SCO0365 (15E17)_PCR/6229 [Gene]SCO0365/5674 NC_003888 SCF41.24, probable oxidoreductase (putative secreted protein), len: 248 aa. Similar to Rhizobium sp. (strain NGR234) SW:Y4LA_RHISN (EMBL; AE000082) putative short-chain type dehydrogenase/reductase Y4LA (EC 1.-.-.-), fasta scores opt: 487 z-score: 537.0 E(): 1.5e-22 38.8% identity in 245 aa overlap. Contains a Pfam match to entry PF00106 adh_short, short chain dehydrogenase, Pfam match to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and a PS00061 Short-chain dehydrogenases/reductases family signature 1 4 9 6 [Reporter]SCO0500 (16A17)_PCR/6228 [Gene]SCO0500/5673 NC_003888 SCF34.19c, unknown, len: 79 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference, amino acid composition and the presence of a potential RBS 1 4 9 5 [Reporter]SCO4874 (17M13)_PCR/6227 [Gene]SCO4874/5672 NC_003888 SCK20.15, possible integral membrane protein, len: 961 aa. Contains match to Prosite entry PS01125 ROK family signature. Also contains 4x degenerate repeat: DRA(S/A)G and possible hydrophobic membrane spanning regions 4 2 15 16 [Reporter]SCO5697 (7D11)_PCR/3851 [Gene]SCO5697/3499 NC_003888 SC5H4.21, hypothetical protein, len: 287 aa; similar to TR:O33349 (EMBL:AL008883) Mycobacterium tuberculosis hypothetical 30.3 kD protein MTV003.13c, 284 aa; fasta scores: opt: 798 z-score: 942.8 E(): 0; 48.0% identity in 269 aa overlap 1 4 9 4 [Reporter]SCO6300 (18I13)_PCR/6226 [Gene]SCO6300/5671 NC_003888 SCIF3.02c, probable secreted hydrolase, len: 615 aa; similar to SW:HEXA_ALTSO (EMBL:D17399) Alteromonas sp. beta-hexosaminidase A precursor (EC 3.2.1.52) Cht60, 598 aa; fasta scores: opt: 565 z-score: 617.5 E(): 7.4e-27; 33.8% identity in 550 aa overlap and to TR:Q9RDG9 (EMBL:AL136519) Streptomyces coelicolor beta-N-acetylglucosaminidase NagA, 615 aa; fasta scores: opt: 2497 z-score: 2289.5 E(): 0; 65.6% identity in 605 aa overlap. Contains Pfam match to entry PF00933 Glyco_hydro_3, Glycosyl hydrolase family 3 N terminal domain and PF01915 Glyco_hydro_3_C, Glycosyl hydrolase family 3 C terminal domain and match to Prosite entry PS00775 Glycosyl hydrolases family 3 active site. Also contains possible N-terminal region signal peptide sequence 4 2 15 15 [Reporter]SCO2479 (8P7)_PCR/3850 [Gene]SCO2479/3498 NC_003888 SC7A8.18, possible membrane protein, len: 180 aa. Contains possible hydrophobic membrane spanning regions both at N- and C-terminal domains 1 4 9 3 [Reporter]SCO4897 (19E13)_PCR/6225 [Gene]SCO4897/5670 NC_003888 2SCK8.23, probable transcriptional regulator, len: 317 aa; similar to TR:Q9KIF0 (EMBL:AF235504) Streptomyces hygroscopicus var. ascomyceticus transcription regulator FkbR1, 297 aa; fasta scores: opt: 726 z-score: 799.4 E(): 0; 45.8% identity in 286 aa overlap. Contains possible helix-turn-helix motif at residues 38..59 (+4.22 SD) 4 2 15 14 [Reporter]SCO6976 (9L7)_PCR/3849 [Gene]SCO6976/3497 NC_003888 SC8F11.02c, unknown, len: 298 aa. Similar to the Bacillus subtilis SW:IOLB_BACSU(EMBL:D14399) hypothetical protein, IolB, found within the inositol utilisation operon (271 aa), fasta scores opt: 443 z-score: 516.6 E(): 2.4e-21 34.9% identity in 281 aa overlap 4 2 15 12 [Reporter]SCO2538 (11D7)_PCR/3847 [Gene]SCO2538/3496 NC_003888 SCC77.05, unknown, len: 132 aa. Similar to Mycobacterium tuberculosis TR:O05833(EMBL:Z95208) hypothetical 11.1 KD protein (113 aa), fasta scores opt: 254 z-score: 298.3 E(): 3.2e-09 53.1% identity in 96 aa overlap and Mycobacterium leprae TR:Q49767(EMBL:U00016) hypothetical 11.1 KD protein (108 aa), fasta scores opt: 186 z-score: 224.0 E(): 4.4e-05 39.4% identity in 99 aa overlap. 4 2 15 11 [Reporter]SCO4131 (12P3)_PCR/3846 [Gene]SCO4131/3495 NC_003888 SCD72A.17c, possible membrane protein, len: 420aa; contains possible membrane-spanning hydrophobic region. 4 2 15 10 [Reporter]SCO3042 (13L3)_PCR/3845 [Gene]SCO3042/3494 NC_003888 SCE34.23, possible transcriptional regulator, len: 396 aa; similar to TR:O86796 (EMBL:AL031317) Streptomyces coelicolor putative transcriptional regulator SC6G4.33, 360 aa; fasta scores: opt: 792 z-score: 875.9 E(): 0; 43.3% identity in 363 aa overlap, to TR:O68907 (EMBL:AF05830) Streptomyces roseofulvus FrnA, 770 aa; fasta scores: opt: 686 z-score: 739.2 E(): 0; 41.3% identity in 283 aa overlap, to Streptomyces coelicolor SCE34.26, 507 aa; fasta scores: opt: 709 z-score: 659.1 E(): 3.4e-31; 36.4% identity in 357 aa overlap, StE34.25, 507 aa; fasta scores: opt: 671 z-score: 624.0 E(): 3e-29;33.2% identity in 365 aa overlap, to SCE34.24, 472 aa; fasta scores: opt: 666 z-score: 619.9 E(): 5.2e-29; 38.0% identity in 324 aa overlap and to SCE34.27 (partial), >356 aa; fasta scores: opt: 554 z-score: 518.2 E(): 2.4e-23; 35.7% identity in 361 aa overlap. Contains a possible hydrophobic membrane spanning region at N-terminal domain 4 2 15 9 [Reporter]SCO3401 (14H3)_PCR/3844 [Gene]SCO3401/3493 NC_003888 SCE9.08, folK, probable hydroxymethyldihydropteridine pyrophosphokinase, len: 203 aa; similar to many e.g. SW:HPPK_ECOLI (EMBL:L06495), FolK, Escherichia coli 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (158 aa), fasta scores; opt: 351 z-score: 426.9 E(): 1.9e-16, 42.0% identity in 138 aa overlap. Putative start codon suggested by GC frame plot and the presence of a potential RBS, resulting in a short N-terminal extension not present in homologs. There is an alternative start codon at position 17. Contains Pfam match to entry PF01288 HPPK, 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK), score 145.80, E-value 7.5e-40 4 2 15 8 [Reporter]SCO1154 (15D3)_PCR/3843 [Gene]SCO1154/3492 NC_003888 SCG8A.08c, unknown, len: 323aa 4 2 15 7 [Reporter]SCO3763 (15P23)_PCR/3842 [Gene]SCO3763/3491 NC_003888 SCH63.10c, hypothetical protein, len: 694 aa; N-terminal region similar to SW:TERD_ALCSP (EMBL:M20238) Alcaligenes sp. tellurium resistance protein TerD (plasmid determined), 192 aa; fasta scores: opt: 420 z-score: 446.6 E(): 2.3e-17; 39.8% identity in 186 aa overlap and C-terminal region similar to C-terminal region of TR:Q9X843 (EMBL:AL049727) Streptomyces coelicolor putative transferase (fragment) SC9B1.24, 317 aa; blastp scores: Score = 76 (26.8 bits), Expect = 7.3, Sum P(2) = 1.0; Identities = 52/165 (31%), Positives = 72/165 (43%). Contains Pfam match to entry PF02342 TerD at N-terminal region 4 2 15 6 [Reporter]SCO1149 (16L23)_PCR/3841 [Gene]SCO1149/3490 NC_003888 SCG8A.03, unknown, len: 156aa; strongly similar to another hypothetical protein from Streptomyces coelicolor TR:CAB69691 (EMBL:AL137165) (158 aa) fasta scores; opt: 570, z-score: 640.3, E(): 3.2e-28, 59.5% identity in 153 aa overlap. Hydrophilic 1 4 8 22 [Reporter]SCO4207 (1M5)_PCR/6222 [Gene]SCO4207/5669 NC_003888 2SCD46.21, conserved hypothetical protein, len: 155aa; similar to TR:Q98CG5 (EMBL:AP003005) MLR5159 protein from Rhizobium loti (Mesorhizobium loti) fasta scores; opt: 419, Z-score: 514.2, E(): 5.2e-21, 47.368% identity (47.368% ungapped) in 133 aa overlap. Note possible alternative downstream translational start site. 1 4 8 21 [Reporter]SCO5376 (2I5)_PCR/6221 [Gene]SCO5376/5668 NC_003888 2SC6G5.20c, chiC, chitinase C (EC 3.2.1.14) (putative secreted protein), len: 609aa; previously sequenced as TR:Q9Z9M8 (EMBL:AB017010). Strongly similar to others from Streptomyces spp. e.g. SW:P11220 (CHIT_STRPL) chitinase 63 precursor from Streptomyces plicatus (610 aa) fasta scores; opt: 3997, z-score: 3833.9, E(): 0, 95.1% identity in 611 aa overlap. Contains Pfam match to entry PF00704 Glyco_hydro_18, Glycosyl hydrolases family 18, Pfam match to entry PF00041 fn3, Fibronectin type III domain, Pfam match to entry PF00553 CBD_2, Cellulose binding domain, Prosite match to PS00561 Cellulose-binding domain, bacterial type, Prosite match to PS01095 Chitinases family 18 active site and Prosite match to PS00018 EF-hand calcium-binding domain. Also contains a possible non-cleavable N-terminal signal sequence. 1 4 8 19 [Reporter]SCO6919 (4A5)_PCR/6219 [Gene]SCO6919/5667 NC_003888 SC1B2.25c, unknown, len: 171 aa. The N-terminus of the predicted protein product of this CDS is hydrophilic. 1 4 8 18 [Reporter]SCO6839 (5M1)_PCR/6218 [Gene]SCO6839/5666 NC_003888 SC3D9.07c, unknown, len: 437 aa. 1 4 8 17 [Reporter]SCO5764 (6I1)_PCR/6217 [Gene]SCO5764/5665 NC_003888 SC4H8.03, putative membrane protein, len: 102 aa. Contains possible hydrophobic membrane spanning regions 1 4 8 16 [Reporter]SCO0297 (7E1)_PCR/6216 [Gene]SCO0297/5664 NC_003888 SC5G9.06, possible secreted protein, len: 425 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains a probable N-terminal signal sequence 1 4 8 15 [Reporter]SCO2140 (8A1)_PCR/6215 [Gene]SCO2140/5663 NC_003888 SC6G10.13, possible transcriptional regulator, len: 93 aa; similar to many e.g. TR:O28868 (EMBL:AE001007) transcriptional regulatory protein, AsnC family from Archaeoglobus fulgidus (77 aa) fasta scores; opt: 148, z-score: 216.7, E(): 9.1e-05, (40.8% identity in 71 aa overlap) and SW: LRP_ECOLI Lrp, leucine responsive regulatory protein from Escherichia coli (163 aa) fasta scores; opt: 107, z-score: 157.6, E(): 0.18, (24.7% identity in 85 aa overlap). 1 4 8 14 [Reporter]SCO7188 (8M21)_PCR/6214 [Gene]SCO7188/5662 NC_003888 SC8A11.16c, possible secreted peptidase, len: 1210 aa; similar to TR:CAB86111 (EMBL:AL163003) Streptomyces coelicolor putative secreted peptidase SCC24.17c, 1220 aa; fasta scores: opt: 2418 z-score: 2421.7 E(): 0; 41.6% identity in 1257 aa overlap, to SW:SUBT_BACAM (EMBL:K02496) Bacillus amyloliquefaciens subtilisin BPM' precursor (EC 3.4.21.62) Apr, 382 aa; fasta scores: opt: 478 z-score: 483.8 E(): 1.8e-19; 41.4% identity in 290 aa overlap and to Streptomyces coelicolor possible secreted peptidase SC8A11.04c, 1253 aa; fasta scores: opt: 1835 z-score: 1440.0 E(): 0; 46.9% identity in 1279 aa overlap. Contains Pfam matches to entries PF00082 Peptidase_S8, Subtilase family and PF02225 PA, PA domain and matches to Prosite entries PS00136 Serine proteases, subtilase family, aspartic acid active site, PS00137 Serine proteases, subtilase family, histidine active site and PS00138 Serine proteases, subtilase family, serine active site. Also contains possible N-terminal region signal peptide sequence 4 2 15 5 [Reporter]SCO0464 (17H23)_PCR/3840 [Gene]SCO0464/3489 NC_003888 SCF76.04c, possible integral membrane protein, len: 565 aa. Similar in parts to many hypothetical proteins e.g. Streptomyces lividans TR:O86875 (EMBL; U22894) hypothetical 57.9 KD protein (547 aa), fasta scores opt: 689 z-score: 806.6 E(): 0 41.7% identity in 439 aa overlap and Streptomyces coelicolor TR:O86807 (EMBL; AL031031) putative regulatory protein (916 aa), fasta scores opt: 329 z-score: 381.2 E(): 7.1e-14 26.5% identity in 582 aa overlap. Contains a Pfam match to entry PF01590 GAF, GAF domain and possible membrane spanning hydrophobic domains. 1 4 8 13 [Reporter]SCO6460 (9I21)_PCR/6213 [Gene]SCO6460/5661 NC_003888 SC9B5.27, possible cation-transporting ATPase, len: 638aa; similar to many cation-transporting ATPases eg. SW:COPA_ENTHR copper /potassium-transporting= from Enterococcus hirae (727 aa) fasta scores: opt: 980, z-score: 936.0, E(): 0, (31.9% identity in 630 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site and Pfam match to entry PF00122 E1-E2_ATPase, E1-E2 ATPases. 4 2 15 4 [Reporter]SCO1071 (18D23)_PCR/3839 [Gene]SCO1071/3488 NC_003888 SCG22.17c, possible two component system sensor kinase, len: 415 aa; similar to TR:Q9X8S1 (EMBL:AL049754) Streptomyces coelicolor putative two component system sensor kinase SCH10.32c, 441 aa; fasta scores: opt: 723 z-score: 761.0 E(): 0; 40.6% identity in 382 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 8 12 [Reporter]SCO2422 (10E21)_PCR/6212 [Gene]SCO2422/5660 NC_003888 SCC42.03c, possible ABC-transporter ATP-binding protein, len: 343 aa; similar to SW:YRBF_HAEIN (EMBL:U32788) Haemophilus influenzae hypothetical ABC-transporter ATP-binding protein HI1087, 264 aa; fasta scores: opt: 680 z-score: 686.6 E(): 8.9e-31; 41.4% identity in 244 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 4 2 15 3 [Reporter]SCO6100 (19P19)_PCR/3838 [Gene]SCO6100/3487 NC_003888 SCBAC1A6.24c, cysH, phosphoadenosine phosphosulfate reductase, len: 236 aa; similar to SW:CYH1_BACSU (EMBL:U76751) Bacillus subtilis phosphoadenosine phosphosulfate reductase (EC 1.8.99.4) CysH, 233 aa; fasta scores: opt: 618 z-score: 746.2 E(): 0; 44.3% identity in 210 aa overlap. Contains Pfam match to entry PF01507 PAPS_reduct, Phosphoadenosine phosphosulfate reductase family 4 2 15 2 [Reporter]SCO5106 (20H19)_PCR/3837 [Gene]SCO5106/3486 NC_003888 SCBAC31E11.02c, shdB2, probable succinate dehydrogenase iron-sulfur subunit, len: 259 aa; highly similar to TR:AAK44479 (EMBL:AE006934) Mycobacterium tuberculosis ferredoxin, 2Fe-2S MT0261, 248 aa; fasta scores: opt: 1355 Z-score: 1588.1 bits: 301.4 E(): 7.7e-81; 74.900% identity in 251 aa overlap and to SW:DHSB_BACSU (EMBL:M13470) Bacillus subtilis succinate dehydrogenase iron-sulfur protein SdhB, 252 aa; fasta scores: opt: 365 Z-score: 434.1 bits: 87.9 E(): 1.4e-16; 30.603% identity in 232 aa overlap. Contains Pfam match to entry PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains 4 2 14 22 [Reporter]SCO1004 (1H15)_PCR/3835 [Gene]SCO1004/3485 NC_003888 2SCG2.17c, unknown, len: 266 aa; similar to part of TR:CAC01640 (EMBL:AL391072) Streptomyces coelicolor conserved hypothetical protein SC9A4.13, 267 aa; fasta scores opt: 191 z-score: 213.7 E(): 0.00022; 52.5% identity in 59 aa overlap. Contains a TTA leucine codon, possible target for bldA regulation 4 2 14 21 [Reporter]SCO5471 (2D15)_PCR/3834 [Gene]SCO5471/3484 NC_003888 SC2A11.05c, gcvH, probable glycine cleavage system H protein, len: 125 aa; highly similar to e.g. GCSH_ECOLI glycine cleavage system H protein (129 aa), fasta scores; opt: 460 z-score: 754.1 E(): 0, 56.0% identity in 125 aa overlap. Contains PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site 4 2 14 20 [Reporter]SCO5922 (3P11)_PCR/3833 [Gene]SCO5922/3483 NC_003888 SC10A5.27c, possible oxidoreductase, len: 326 aa; some similarity to many members of the SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY (SDR) eg. YGFF_ECOLI P52037 hypothet ical oxidoreductase (247 aa), fasta scores; opt: 96 z-score : 310.6 E(): 4.2e-10, 29.1% identity in 213 aa overlap. Con tains PS00017 ATP/GTP-binding site motif A (P-loop) 4 2 14 19 [Reporter]SCO7022 (4L11)_PCR/3832 [Gene]SCO7022/3482 NC_003888 SC1H10.11, unknown, len: 310 aa. 4 2 14 18 [Reporter]SCO5457 (5H11)_PCR/3831 [Gene]SCO5457/3481 NC_003888 SC3D11.14, possible lipoprotein, len: 416aa; similar to many eg. TR:O53223 (EMBL:AL021185) hypothetical protein from Mycobacterium tuberculosis (408 aa) fasta scores; opt: 737, z-score: 800.7, E(): 0, 32.9% identity in 413 aa overlap. Also similar to neighbouring CDS SC3D11.15 fasta scores; opt: 1348, z-score: 1271.2, E(): 0, 53.3% identity in 394 aa overlap. Contains possible N-terminal signal sequence and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site 4 2 14 17 [Reporter]SCO5798 (6D11)_PCR/3830 [Gene]SCO5798/3480 NC_003888 SC4H2.19c, probable secreted protein, len: 409 aa; Contains probable N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Highly similar to downstream CDS SC4H2.18c (405 aa), fasta scores; E(): 0, 61.8% identity in 406 aa overlap 1 4 8 11 [Reporter]SCO4072 (11A21)_PCR/6211 [Gene]SCO4072/5659 NC_003888 SCD25.08c, luxR family two component system response regulator, len: 200. Highly similar to many e.g. Bacillus subtilis TR:O34723 (EMBL; AF027868), fasta scores opt: 538 z-score: 609.7 E(): 1.3e-26 41.2% identity in 199 aa overlap and Streptomyces coelicolor TR:CAB46973 (EMBL; AL096825) putative two-component system response regulator SC6G3.01C (fragment) (202 aa), fasta scores opt: 413 z-score: 356.7 E(): 1.3e-14 38.6% identity in 202 aa overlap. Contains a Pfam match to entry PF00072 response_reg, Response regulator receiver domain and a Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family. Also contains a possible helix-turn-helix motif between residues 156..177. 1 4 8 10 [Reporter]SCO2912 (12M17)_PCR/6210 [Gene]SCO2912/5658 NC_003888 SCE19A.12c, hypothetical protein, len: 47 aa; unknown function, possible CDS suggested by GC frameplot 1 4 8 9 [Reporter]SCO0285 (13I17)_PCR/6209 [Gene]SCO0285/5657 NC_003888 SCF85.13, possible sodium/proton antiporter, len: 629 aa. Similar to many including Streptomyces coelicolor TR:CAB38501 (EMBL; AL035636) putative sodium/proton antiporter SCH5.27 (474 aa), fasta scores opt: 603 z-score: 691.3 E(): 3.7e-31 37.7% identity in 454 aa overlap. This putative protein is longer than most of the similar antiporter proteins. The additional C-terminal sequence has similarity to many outer membrane proteins e.g. Coxiella burnetii TR:O07645 (EMBL; AB004699) 27KDA outer membrane protein (252 aa), fasta scores opt: 250 z-score: 291.5 E():c7e-09 28.6% identity in 140 aa overlap. Contains membrane spanning hydrophobic domains. 1 4 8 8 [Reporter]SCO4943 (14E17)_PCR/6208 [Gene]SCO4943/5656 NC_003888 2SCK31.03, probable oxidoreductase, len: 330 aa; similar to TR:AAG05923 (EMBL:AE004681) Pseudomonas aeruginosa probable oxidoreductase PA2535, 331 aa; fasta scores: opt: 1001 z-score: 1136.6 E(): 0; 48.2% identity in 328 aa overlap. Contains Pfam match to entry PF00248 aldo_ket_red, Aldo/keto reductase family 1 4 8 7 [Reporter]SCO0905 (15A17)_PCR/6207 [Gene]SCO0905/5655 NC_003888 SCM1.38, possible membrane protein, len: 101 aa. Highly similar to the very C-terminus of Streptomyces coelicolor SW:MMLA_STRCO(EMBL:M64683) putative membrane protein ACTII-3 (711 aa), fasta scores opt: 232 z-score: 254.6 E(): 7.8e-07 48.3% identity in 87 aa overlap. Contains a possible N-terminal signal sequence and a possible membrane spanning hydrophobic domain. 1 4 8 6 [Reporter]SCO0324 (16M13)_PCR/6206 [Gene]SCO0324/5654 NC_003888 SCF12.03c, possible secreted protein, len: 799 aa; similar to TR:CAC45838 (EMBL:AL591786) Rhizobium meliloti (Sinorhizobium meliloti) hypothetical transmembrane protein (fragment) SMC01907, 729 aa; fasta scores: opt: 492 Z-score: 517.1 E(): 3.6e-21; 42.318% identity in 742 aa overlap. Contains possible cleavable N-terminal signal sequence 1 4 8 5 [Reporter]SCO2967 (17I13)_PCR/6205 [Gene]SCO2967/5653 NC_003888 SCE59.26c, probable carboxy-terminal processing protease precursor, len: 374 aa; similar to SW:CTPA_BARBA (EMBL:L37094) Bartonella bacilliformis carboxy-terminal processing protease precursor (C-terminal processing protease) CtpA, 434 aa; fasta scores: opt: 505 z-score: 527.2 E(): 6e-22; 30.6% identity in 337 aa overlap. Contains Pfam match to entry PF00595 PDZ, PDZ domain (Also known as DHR or GLGF) and possible N-terminal region signal peptide sequence 1 4 8 4 [Reporter]SCO3417 (18E13)_PCR/6204 [Gene]SCO3417/5652 NC_003888 SCE9.24c, probable ABC transporter transmembrane component, len: 315 aa; similar to many e.g. SW:DRRB_STRPE (EMBL:M73758), drrB, Streptomyces peucetius daunorubicin resistance transmembrane protein (283 aa), fasta scores; opt: 507 z-score: 565.3 E(): 3.6e-24, 35.6% identity in 264 aa overlap. Contains hydrophobic, possible membrane-spanning regions 4 2 14 16 [Reporter]SCO6765 (7P7)_PCR/3829 [Gene]SCO6765/3479 NC_003888 SC6A5.14, possible lipoprotein, len: 213 aa; similar to SW:TYRT_STRLN proposed tyrosinase co-factor from the melanin biosynthesis in Streptomyces lincolnensis (140 aa) fasta scores; opt: 115, z-score: 142.8, E(): 1.2, (29.2% identity in 120 aa overlap). Contains Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 1 4 8 3 [Reporter]SCO4883 (19A13)_PCR/6203 [Gene]SCO4883/5651 NC_003888 2SCK8.09, probable peptidase, len: 410 aa; similar to SW:CBPX_SULSO (EMBL:Z48497) Sulfolobus solfataricus thermostable carboxypeptidase (EC 3.4.17.*) CpsA, 393 aa; fasta scores: opt: 687 z-score: 803.2 E(): 0; 32.0% identity in 372 aa overlap. Contains Pfam match to entry PF01546 Peptidase_M20, Peptidase family M20/M25/M40 4 2 14 15 [Reporter]SCO2475 (8L7)_PCR/3828 [Gene]SCO2475/3478 NC_003888 SC7A8.14, probable lysR-family transcriptional regulator, len: 309 aa; similar to TR:O06466 (EMBL:U94336) Xanthomonas campestris oxidative stress transcriptional regulator OxyR, 313 aa; fasta scores: opt: 374 z-score: 419.6 E(): 5.5e-16; 29.7% identity in 316 aa overlap and SW:OXYR_MYCLE (EMBL:L01095) Mycobacterium leprae probable hydrogen peroxide-inducible genes activator OxyR, 311 aa; fasta scores: opt: 375 z-score: 420.8 E(): 4.8e-16; 33.7% identity in 246 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Contains a possible helix-turn-helix motif at residues 16..37 (+3.82 SD) 1 4 8 2 [Reporter]SCO2964 (20I9)_PCR/6202 [Gene]SCO2964/5650 NC_003888 SCE59.23c, probable lysR-family transcriptional regulator, len: 323 aa; similar to TR:O88132 (EMBL:AJ006517) Streptomyces lipmanii lysR-type transcriptional regulator StgR, 327 aa; fasta scores: opt: 551 z-score: 619.3 E(): 4.4e-27; 36.6% identity in 320 aa overlap and to SW:XAPR_ECOLI (EMBL:X63976) Escherichia coli xanthosine operon regulatory protein XapR, 294 aa; fasta scores: opt: 364 z-score: 412.4 E(): 1.5e-15; 33.6% identity in 301 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Contains possible helix-turn-helix motif at residues 22..43 (+3.25 SD) 4 2 14 14 [Reporter]SCO7747 (9H7)_PCR/3827 [Gene]SCO7747/3477 NC_003888 SC8D11.38, hypothetical protein, len: 392 aa; similar to TR:Q9L195 (EMBL:AL158057) Streptomyces coelicolor hypothetical 47.1 kDa protein SC10G8.07c, 437 aa; fasta scores: opt: 419 z-score: 494.1 E(): 5.5e-20; 28.5% identity in 393 aa overlap 4 2 14 13 [Reporter]SCO6216 (10D7)_PCR/3826 [Gene]SCO6216/3476 NC_003888 SC9G1.06c, hypothetical protein, len: 140 aa; similar to SW:YBAW_ECOLI (EMBL;AE000150) Escherichia coli hypothetical 15.1 KD protein, 132 aa; fasta scores: opt: 165 z-score: 225.5 E(): 3.5e-05; 26.0% identity in 127 aa overlap 4 2 14 12 [Reporter]SCO2370 (11P3)_PCR/3825 [Gene]SCO2370/3475 NC_003888 SCC8A.28c, unknown, len: 159 aa; similar to others from mycobacteria e.g. SW:Q10521 (YM39_MYCTU) hypothetical protein from Mycobacterium tuberculosis (158 aa) fasta scores; opt: 447, z-score: 518.6, E(): 2e-21, 50.4% identity in 141 aa overlap 4 2 14 11 [Reporter]SCO4127 (12L3)_PCR/3824 [Gene]SCO4127/3474 NC_003888 SCD72A.13c, possible ATP/GTP-binding protein, len: 471 aa; similar to TR:CAB72383 (EMBL:AL138977) putative ATP-binding protein from Streptomyces coelicolor (507 aa) fasta scores; opt: 618, z-score: 740.6, E(): 0, 32.9% identity in 434 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) 4 2 14 10 [Reporter]SCO3195 (13H3)_PCR/3823 [Gene]SCO3195/3473 NC_003888 SCE22.12, unknown, len: 226 aa 3 1 19 16 [Reporter]SCO6177 (7C16)_PCR/1419 [Gene]SCO6177/1299 NC_003888 SC6C5.13c, unknown, len: 327aa 4 2 14 9 [Reporter]SCO3775 (14D3)_PCR/3822 [Gene]SCO3775/3472 NC_003888 SCH63.22c, unknown, len: 577 aa. High content in glycine and alanine amino acid residues 3 1 19 15 [Reporter]SCO5760 (8O12)_PCR/1418 [Gene]SCO5760/1298 NC_003888 SC7C7.15c, probable DNA glycosylase involved in DNA repair, len: 276 aa; similar to e.g. END8_ECOLI endonuclease VIII (EC 3.2.-.-) (263 aa), fasta scores; opt: 384 z-score: 449.2 E(): 8.7e-18, 34.5% identity in 278 aa overlap 4 2 14 7 [Reporter]SCO3586 (15L23)_PCR/3820 [Gene]SCO3586/3471 NC_003888 SCH66.07c, possible integral membrane protein, len: 330aa; contains a possible membrane spanning hydrophobic region 3 1 19 14 [Reporter]SCO5404 (9K12)_PCR/1417 [Gene]SCO5404/1297 NC_003888 SC8F4.08, probable two-component sensor kinase, len: 478 aa; similar to many proposed two-component sensor kinases eg. SW:P18392 (RSTB_ECOLI) sensor protein RstB from Escherichia coli (433 aa) fasta scores; opt: 379, z-score: 410.8, E(): 1.8e-15, 35.6% identity in 239 aa overlap. Contains Pfam match to entry PF00672 DUF5, Domain found in bacterial signal proteins and Pfam match to entry PF00512 signal, Histidine kinase. 4 2 14 6 [Reporter]SCO3407 (16H23)_PCR/3819 [Gene]SCO3407/3470 NC_003888 SCE9.14c, conserved hypothetical protein, len: 375 aa; unknown function, highly similar to TR:O06381 (EMBL:Z95436) Mycobacterium tuberculosis hypothetical protein (350 aa), fasta scores; opt: 720 z-score: 820.9 E(): 0, 41.6% identity in 361 aa overlap and similar to TR:O53341 (EMBL:AL021646) Mycobacterium tuberculosis hypothetical protein (472 aa) (25.6% identity in 383 aa overlap) and TR:Q49746 (EMBL:U00016) Mycobacterium leprae hypothetical protein (479 aa) (24.9% identity in 305 aa overlap) 3 1 19 13 [Reporter]SCO6245 (10G12)_PCR/1416 [Gene]SCO6245/1296 NC_003888 SCAH10.10, unknown, len: 104 aa 3 1 19 12 [Reporter]SCO2604 (11C12)_PCR/1415 [Gene]SCO2604/1295 NC_003888 SCC88.15c, possible secreted protein, len: 311 aa. Contains N-terminal region signal peptide sequence and TTA leucine codon, possible target for bldA regulation 3 1 19 11 [Reporter]SCO4640 (12O8)_PCR/1414 [Gene]SCO4640/1294 NC_003888 SCD82.11, possible tetR-family transcriptional regulator, len: 197 aa; similar to TR:AAF11696 (EMBL:AE002049) Deinococcus radiodurans transcriptional regulator, tetR family DR2148, 204 aa; fasta scores: opt: 175 z-score: 223.6 E(): 4.9e-05; 26.2% identity in 172 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 3 1 19 10 [Reporter]SCO3095 (13K8)_PCR/1413 [Gene]SCO3095/1293 NC_003888 SCE41.04c, putative membrane protein, len: 174 aa; similar to TR:P96376 (EMBL:Z92539) Mycobacterium tuberculosis hypothetical 24.6 kDa protein MTC10G2.25c, 228 aa; fasta scores: opt: 273 z-score: 332.6 E(): 5.1e-11; 35.6% identity in 149 aa overlap. Contains possible coiled-coil region at approx residues 87..106 and possible hydrophobic membrane spanning regions 3 1 19 9 [Reporter]SCO6799 (14G8)_PCR/1412 [Gene]SCO6799/1292 NC_003888 SC1A2.08, tdh, threonine 3-dehydrogenase (EC 1.1.1.103), len: 342 aa. Highly similar to many dehydrogenases involved in L-threonine catabolism e.g. Escherichia coli SW:TDH_ECOLI(EMBL:X06690) threonine 3-dehydrogenase (EC 1.1.1.103) (341 aa), fasta scores opt: 1464 z-score: 1579.9 E(): 0 60.5% identity in 342 aa overlap. Contains a Prosite hit to PS00059 Zinc-containing alcohol dehydrogenases signature and a Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases. 3 1 19 8 [Reporter]SCO5621 (15C8)_PCR/1411 [Gene]SCO5621/1291 NC_003888 SC2E1.38, whiG, RNA polymerase sigma factor WhiG, len: 280 aa; identical to RPSW_STRCO RNA polymerase sigma factor WhiG (280 aa). Also highly similar to RPSD_BACSU RNA polymerase sigma-D factor (sigma-28) (254 aa)(E(): 0, 43.0% identity in 242 aa overlap) and FLIA_ECOLI RNA polymerase sigma factor for flagellar operon (sigma F) (239 aa)(E(): 0, 42.8% identity in 229 aa overlap). Contains PS00715 and PS00716 Sigma-70 factors family signatures 1 and 2, Pfam match to entry sigma70 PF00140, Sigma-70 factors, score 342.03 and probable helix-turn-helix motif at aa 245 to 266 (Score 1657, +4.83 SD) 3 1 19 7 [Reporter]SCO0410 (16O4)_PCR/1410 [Gene]SCO0410/1290 NC_003888 SCF51.09c, hypothetical protein, len: 416 aa; similar to TR:Q49897 (EMBL:U00021) Mycobacterium leprae hypothetical protein MLC1351.13c, 311 aa; fasta scores: opt: 288 z-score: 317.7 E(): 2.5e-10; 34.4% identity in 326 aa overlap. Contains Pfam match to entry PF01513 DUF15, domain of unknown function and prosite PS00339 aminoacyl-transfer RNA synthetases class-II signature 2 1 4 7 22 [Reporter]SCO4203 (1I5)_PCR/6200 [Gene]SCO4203/5649 NC_003888 2SCD46.17c, unknown, len: 271aa; 1 4 7 21 [Reporter]SCO0784 (2E5)_PCR/6199 [Gene]SCO0784/5648 NC_003888 3SCF60.16, possible secreted protein, len: 227 aa. Contains possible N-terminal region signal peptide sequence 1 4 7 20 [Reporter]SCO6515 (3A5)_PCR/6198 [Gene]SCO6515/5647 NC_003888 SC1E6.24c, pfpI, probable protease, len: 180 aa; similar to e.g. PFPI_PYRFU protease I (EC 3.2.-.-) (166 aa), fasta scores; opt: 413 z-score: 411.9 E(): 1.2e-15, 41.3% identity in 172 aa overlap 1 4 7 19 [Reporter]SCO6036 (4M1)_PCR/6197 [Gene]SCO6036/5646 NC_003888 SC1C3.24, unknown, len: 189 aa 1 4 7 18 [Reporter]SCO5466 (5I1)_PCR/6196 [Gene]SCO5466/5645 NC_003888 SC3D11.23c, possible hydrolase, len: 185aa; similar to many eg. SW:P34020 (LYC_CLOAB) autolytic lysozyme from Clostridium acetobutylicum (324 aa) fasta scores; opt: 231, z-score: 295.8, E(): 4.6e-09, 29.3% identity in 188 aa overlap. 1 4 7 17 [Reporter]SCO7334 (6E1)_PCR/6195 [Gene]SCO7334/5644 NC_003888 SC4G10.13c, possible trehalose synthase, len: 572 aa. Previously sequenced region :Streptomyces coelicolor TR:O54207(EMBL:AJ001206) putative trehalose synthase (572 aa). Highly similar to Streptomyces coelicolor TR:CAB72418(EMBL:AL138978) putative alpha-amylase, SC6A11.18C (566 aa), fasta scores opt: 3717 z-score: 4344.1 E():0 92.2% identity in 566 aa overlap and Pimelobacter sp.TR:P72235(EMBL:D78198) trehalose synthase (573 aa), fasta scores opt: 2633 z-score: 3076.9 E():0 73.2% identity in 560 aa overlap. Contains a Pfam match to entry PF00128 alpha-amylase, Alpha amylase and a Prosite hit to PS01034 Glycosyl hydrolases family 16 active sites. 1 4 7 16 [Reporter]SCO4860 (7A1)_PCR/6194 [Gene]SCO4860/5643 NC_003888 SCK20.01, probable secreted hydrolase (fragment), len: >465 aa; similar to C-terminal region of SW:HEXA_DICDI (EMBL:J04065) Dictyostelium discoideum beta-hexosaminidase A precursor (EC 3.2.1.52) NagA, 532 aa; fasta scores: opt: 343 z-score: 387.7 E(): 4.6e-14; 25.4% identity in 393 aa overlap. Contains Pfam match to entry PF00728 Glyco_hydro_20, Glycosyl hydrolase family 20,SC5G8.28, partial CDS, possible secreted protein, len: >94aa; contains a possible cleavable N-terminal signal sequence. 1 4 7 15 [Reporter]SCO6956 (7M21)_PCR/6193 [Gene]SCO6956/5642 NC_003888 SC6F7.09, possible oxidoreductase, len: 579 aa. Similar to many including: Rhodobacter capsulatus SW:NUON_RHOCA(EMBL:U25800) NADH dehydrogenase I chain N (EC 1.6.5.3) (478 aa), fasta scores opt: 538 z-score: 523.8 E(): 9.6e-22 29.1% identity in 385 aa overlap. Also similar to Bacillus firmus multiple resistance and pH regulation related protein D (493 aa), fasta scores opt: 1171 z-score: 1130.8 E(): 0 43.8% identity in 441 aa overlap. Also weakly similar to CDS SC6F7.07 also carried on this cosmid, fasta scores opt: 476 z-score: 387.6 E(): 4.5e-16 28.2% identity in 444 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a Pfam match to entry PF00361 oxidored_q1, NADH-Ubiquinone/plastoquinone (complex I), various chains. Note contains a 3xGSGEAS repeat towards the C-terminus. 4 2 14 5 [Reporter]SCO3131 (17D23)_PCR/3818 [Gene]SCO3131/3469 NC_003888 SCE66.10c, unknown, len: 125aa; similar to TR:Q9RY42 (EMBL:AE001873) hypothetical protein from Deinococcus radiodurans 1 4 7 14 [Reporter]SCO7184 (8I21)_PCR/6192 [Gene]SCO7184/5641 NC_003888 SC8A11.12, probable branched amino acid transport system permease, len: 349 aa; similar to SW:LIVM_ECOLI (EMBL:J05516) Escherichia coli high-affinity branched-chain amino acid transport system permease, LivM, 425 aa; fasta scores: opt: 254 z-score: 285.7 E(): 1.9e-08; 28.1% identity in 360 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 14 4 [Reporter]SCO6105 (18P19)_PCR/3817 [Gene]SCO6105/3468 NC_003888 SCBAC1A6.29c, possible regulatory protein, len: 429 aa; N-terminal region similar to N-terminal region of TR:Q9RJZ7 (EMBL:AL133210) Streptomyces coelicolor putative transcriptional regulator SCF37.04, 419 aa; fasta scores: opt: 520 z-score: 594.4 E(): 1.6e-25; 40.8% identity in 240 aa overlap 1 4 7 13 [Reporter]SCO7171 (9E21)_PCR/6191 [Gene]SCO7171/5640 NC_003888 SC9A4.33c, possible lyase, len: 143 aa; similar to TR:O86701 (EMBL:AL031515) Streptomyces coelicolor putative lyase SC5C7.04, 138 aa; fasta scores: opt: 119 z-score: 158.2 E(): 0.24; 30.6% identity in 134 aa overlap 4 2 14 3 [Reporter]SCP1.92 (19L19)_PCR/3816 [Gene]SCP1.92/3467 NC_003888 SCP1.92, unknown, len: 202aa; 4 2 14 2 [Reporter]SCO5966 (20D19)_PCR/3815 [Gene]SCO5966/3466 NC_003888 SCBAC16H6.01c, possible oxidase, len: >1032aa: similar to many of undefined function eg. TR:Q9EZK2 (EMBL:AF289509) putative oxidase YdiJ-like protein from Pseudomonas putida (1006 aa) fasta scores; opt: 880, Z-score: 889.5, 27.930% identity (31.672% ungapped) in 1024 aa overlap and TR:Q9F2Y0 (EMBL:AL392149) putative oxidoreductase SCD19.22 from Streptomyces coelicolor (951 aa) fasta scores; opt: 622, Z-score: 628.7, 49.698% identity (53.813% ungapped) in 994 aa overlap. Contains Prosite match to PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature; Pfam match to entry PF02913 FAD-oxidase_C, FAD linked oxidases, C-terminal domain and Pfam match to entry PF01565 FAD_binding_4, FAD binding domain.,SC7H1.36c, unknown, partial CDS, len: >81 aa 4 2 13 22 [Reporter]SCO0996 (1D15)_PCR/3813 [Gene]SCO0996/3465 NC_003888 2SCG2.09, possible lipoprotein, len: 347 aa; similar to TR:Q9K467 (EMBL:AL359215) Streptomyces coelicolor putative iron transport lipoprotein SC2H12.17, 345 aa; fasta scores: opt: 473 z-score: 540.7 E(): 1.4e-22; 39.2% identity in 339 aa overlap. Contains Pfam match to entry PF01497 Peripla_BP_2, Periplasmic binding protein, correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 4 2 13 21 [Reporter]SCO2388 (2P11)_PCR/3812 [Gene]SCO2388/3464 NC_003888 SC4A7.16, fabH, 3-oxoacyl-[acyl-carrier-protein] synthase III, len: 343 aa; identical to previously sequenced TR:P72392 (EMBL:X86475) Streptomyces coelicolor beta-ketoacyl-acyl carrier protein synthase III FabH, 343 aa and similar to SW:FABH_MYCTU (EMBL:Z95558) Mycobacterium tuberculosis 3-oxoacyl-[acyl-carrier-protein] synthase III (EC 2.3.1.41) (KAS III) FabH, 335 aa; fasta scores: opt: 659 z-score: 720.9 E(): 9e-33; 50.1% identity in 343 aa overlap and to S. coelicolor SC3F7.08, 335 aa; fasta scores: opt: 707 z-score: 718.9 E(): 1.2e-34; 39.5% identity in 332 aa overlap 4 2 13 20 [Reporter]SCO5915 (3L11)_PCR/3811 [Gene]SCO5915/3463 NC_003888 SC10A5.20, unknown, len: 171 aa; weak similarity to part of TR:P79078 (EMBL:Y10421) DELTA-9 FATTY ACID DESATURA SE (EC 1.14.99.5) from CRYPTOCOCCUS CURVATUS (555 aa), fast a scores; opt: 83 z-score: 221.8 E(): 3.7e-05, 25.1% identi ty in 171 aa overlap 3 1 19 6 [Reporter]SCO5027 (17K4)_PCR/1409 [Gene]SCO5027/1289 NC_003888 SCK15.29c, hypothetical protein, len: 397 aa; similar to TR:Q9RKN1 (EMBL:AL121850) Streptomyces coelicolor hypothetical 37.4 kD protein (fragment) SCC78.05, 347 aa; fasta scores: opt: 180 z-score: 199.8 E(): 0.0012; 28.6% identity in 360 aa overlap 4 2 13 19 [Reporter]SCO6022 (4H11)_PCR/3810 [Gene]SCO6022/3462 NC_003888 SC1C3.10, unknown, len: 540 aa; some similarity to hypothetical proteins e.g. M. tuberculosis TR:O06628 (EMBL:Z95618) MTCY07H7A.03 (532 aa), fasta scores; opt: 151 z-score: 278.3 E(): 2.8e-08, 25.9% identity in 529 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature, though this may be spurious. Also similar to flanking genes SC1C3.11 (E(): 2.5e-20, 29.0% identity in 583 aa overlap) and SC1C3.09 (E(): 1.1e-14, 28.0% identity in 542 aa overlap) 3 1 19 5 [Reporter]SCO3217 (18G4)_PCR/1408 [Gene]SCO3217/1288 NC_003888 SCE8.10, cdaR, transcriptional activator protein, len: 638 aa; Previously sequenced and therefore identical to CdaR (EM:AF124138) transcriptional activator protein for the CDA biosynthesis cluster in Streptomyces coelicolor. Also similar to many including SW:AFSR_STRCO AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2) (993 aa) fasta scores; opt: 972, z-score: 1087.1, E(): 0, (36.6% identity in 610 aa overlap) and SW:REDD_STRCO regulator of undecylprodigiosin production in Streptomyces coelicolor A3(2) (350 aa) fasta scores; opt: 382, z-score: 432.2, E(): 9.1e-17, (32.6% identity in 258 aa overlap). Contains match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 2 13 18 [Reporter]SCO5452 (5D11)_PCR/3809 [Gene]SCO5452/3461 NC_003888 SC3D11.09, probable ABC transporter, ATP-binding subunit, len: 312aa; similar to many eg. TR:Q9XAC8 (EMBL:AL096839) putative ABC transporter, ATP-binding subunit from Streptomyces coelicolor (307 aa) fasta scores; opt: 713, z-score: 795.0, E(): 0, 43.5% identity in 299 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. 3 1 19 4 [Reporter]SCO4019 (19C4)_PCR/1407 [Gene]SCO4019/1287 NC_003888 2SC10A7.23, probable gntR family regulatory protein, len: 230 aa; similar to SW:YBPA_BURCE (EMBL:M86348) Burkholderia cepacia hypothetical transcriptional regulator in BphA 5' region, 245 aa; fasta scores: opt: 381 z-score: 442.5 E(): 3.8e-17; 36.0% identity in 211 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 4 2 13 17 [Reporter]SCO7445 (6P7)_PCR/3808 [Gene]SCO7445/3460 NC_003888 SC5C11.02, unknown, len: 245 aa. Weakly similar to Escherichia coli SW:YJHP_ECOLI(EMBL:U14003) hypothetical 27.4 KD protein (248 aa), fasta scores opt: 301 z-score: 358.4 E(): 1.5e-12 28.2% identity in 248 aa overlap. 3 1 19 3 [Reporter]SCP1.234 (19O24)_PCR/1406 [Gene]SCP1.234/1286 NC_003888 SCP1.234, mmyA, possible acyl carrier protein, len: 82aa; previously sequenced and annotated as hypothetical protein TR:Q9JN83 (EMBL:AJ276673). Similar to many acyl carrier proteins eg. TR:Q54497 (EMBL:AJ224512) acyl carrier protein from Streptomyces nogalater (86 aa) fasta scores; opt: 146, z-score: 218.3, E(): 0.00011, 41.0% identity in 78 aa overlap. Contains Pfam match to entry PF00550 pp-binding, Phosphopantetheine attachment site. 3 1 19 2 [Reporter]SCO5976 (20G24)_PCR/1405 [Gene]SCO5976/1285 NC_003888 SCBAC16H6.11, arcB, ornithine carbamoyltransferase, len: 335 aa; similar to many e.g. SW:O31018 (OTCC_RHIET) ornithine carbamoyltransferase from Rhizobium etli (334 aa) fasta scores; opt: 1401, Z-score: 1585.7, 64.024% identity (64.220% ungapped) in 328 aa overlap. Contains Pfam match to entry PF02729 OTCace_N, Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain; Pfam match to entry PF00185 OTCace, Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain and Prosite match to PS00097 Aspartate and ornithine carbamoyltransferases signature 3 1 18 22 [Reporter]SCO1133 (1G20)_PCR/1403 [Gene]SCO1133/1284 NC_003888 2SCG38.26c, probable oxidoreductase, molybdopterin binding subunit, len: 330 aa; similar to TR:AAG05320 (EMBL:AE004619) Pseudomonas aeruginosa probable hydroxylase molybdopterin-containing subunit PA1932, 329 aa; fasta scores: opt: 1032 z-score: 1156.3 E(): 0; 52.0% identity in 325 aa overlap and to TR:Q9RK26 (EMBL:AL132856) Streptomyces coelicolor possible oxidoreductase, molybdopterin binding subunit SCF15.11, 330 aa; fasta scores: opt: 1254 z-score: 1403.4 E(): 0; 62.0% identity in 326 aa overlap. Contains Pfam match to entry PF00941 dehydrog_molyb, Molybdopterin binding domain in dehydrogenase 3 1 18 21 [Reporter]SCO0652 (2C20)_PCR/1402 [Gene]SCO0652/1283 NC_003888 SCF91.12, gvpG2, possible gas vesicle synthesis protein, len: 92 aa. Similar to Bacillus megaterium TR:O68673(EMBL:AF053765) gas vesicle protein GvpG (88 aa), fasta scores opt: 108 z-score: 155.4 E(): 0.28 31.7% identity in 63 aa overlap and Streptomyces coelicolor TR:Q9ZC11 (EMBL:AL033505) putative gas vesical synthesis protein GvpG (87 aa), fasta scores opt: 216 z-score: 290.9 E(): 7.9e-09 43.7% identity in 87 aa overlap. Contains a probable coiled-coil from 40 to 67 (28 residues). 3 1 18 20 [Reporter]SCO7696 (3O16)_PCR/1401 [Gene]SCO7696/1282 NC_003888 SC1A4.04, possible marR-family transcriptional regulator, len: 151 aa; similar to SW:YS87_MYCTU (EMBL:Z74024) Mycobacterium tuberculosis hypothetical transcriptional regulator RV2887, 139 aa; fasta scores: opt: 354 z-score: 441.2 E(): 4.8e-17; 43.0% identity in 135 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family and possible helix-turn-helix motif at residues 55..76 (+2.75 SD) 3 1 18 19 [Reporter]SCO7224 (4K16)_PCR/1400 [Gene]SCO7224/1281 NC_003888 SC2H12.23c, possible integral membrane protein, len: 151 aa. Highly similar to several proteins of undefined function e.g. Haemophilus influenzae SW:Y886_HAEIN (EMBL:U32770) hypothetical protein (134 aa), fasta scores opt: 232 z-score: 291.2 E(): 9.6e-09 35.6% identity in 135 aa overlap and Streptomyces coelicolor TR:CAB92826 (EMBL:AL356892) putative integral membrane protein, SCC8A.08 (153 aa), fasta scores opt: 533 z-score: 650.1 E(): 9.8e-29 58.7% identity in 143 aa overlap. Contains possible membrane spanning hydrophobic domains 3 1 18 18 [Reporter]SCO2401 (5G16)_PCR/1399 [Gene]SCO2401/1280 NC_003888 SC4A7.29, possible dehydratase, len: 388 aa; similar to C-terminal region of SW:DGOA_ECOLI (EMBL:L10328) Escherichia coli galactonate dehydratase (EC 4.2.1.6) GdoA, 587 aa; fasta scores: opt: 751 z-score: 864.7 E(): 0; 35.0% identity in 383 aa overlap. Contains Pfam match to entry PF01188 MR_MLE, Mandelate racemase / muconate lactonizing enzyme family 1 4 7 12 [Reporter]SCO2309 (10A21)_PCR/6190 [Gene]SCO2309/5639 NC_003888 SCC30.17c, probable transmembrane transport protein, len: 489 aa; similar to TR:Q9WVV3 (EMBL:AF127374) Streptomyces lavendulae Mtc, 484 aa; fasta scores: opt: 1680 z-score: 1711.3 E(): 0; 54.7% identity in 475 aa overlap and to SW:CMCT_NOCLA (EMBL:Z13973) Nocardia lactamdurans cephamycin export protein CmcT, 486 aa; fasta scores: opt: 1334 z-score: 1360.2 E(): 0; 48.3% identity in 480 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 7 11 [Reporter]SCO4525 (11M17)_PCR/6189 [Gene]SCO4525/5638 NC_003888 SCD35.32, unknown, len: 293 aa. Contains match to Prosite entry PS00038 Myc-type, 'helix-loop-helix' dimerization domain signature 1 4 7 10 [Reporter]SCO2907 (12I17)_PCR/6188 [Gene]SCO2907/5637 NC_003888 SCE19A.07, possible PTS transmembrane component, len: 416 aa; similar to many PTS (phosphoenolpyruvate-dependent sugar phosphotransferase system) transmembrane and sugar-binding components e.g. SW:PTGB_ECOLI (EMBL:J02618), ptsG, glcA, Escherichia coli PTS system, glucose-specific IIBC component (477 aa), fasta scores; opt: 1112 z-score: 1244.4 E(): 0, 44.7% identity in 396 aa overlap. Similar to the adjacent CDS SCE19A.06, possible PTS transmembrane component (431 aa) (64.1% identity in 429 aa overlap). The homologous PTS family proteins have a C-terminal extension not present in this CDS. Contains hydrophobic, possible membrane-spanning regions 1 4 7 9 [Reporter]SCO1767 (13E17)_PCR/6187 [Gene]SCO1767/5636 NC_003888 SCI51.07c, possible DNA hydrolase, len: 239 aa; shows weak similarity to nucleotide pyrophosphatases e.g. TR:Q58549 (EMBL:U67557) Methanococcus jannaschii ADP-ribose pyrophosphatase (169 aa), fasta scores; opt: 256 z-score: 312.0 E(): 5e-10, 43.8% identity in 112 aa overlap and SW:MUTX_STRMU (EMBL:D78182), MutX, Streptococcus mutans mutator MutT protein (dGTP pyrophosphohydrolase) (159 aa) (30.5% identity in 118 aa overlap). Also similar many hypothetical proteins e.g. TR:O69994 (EMBL:AL022374) S.coelicolor possible DNA hydrolase (294 aa) (56.2% identity in 249 aa overlap) and TR:O06597 (EMBL:Z95586) Mycobacterium tuberculosis hypothetical protein (236 aa) (34.8% identity in 221 aa overlap). Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein and PS00893 mutT domain signature 1 4 7 8 [Reporter]SCO0795 (14A17)_PCR/6186 [Gene]SCO0795/5635 NC_003888 SCF43.06, unknown, len: 164 aa. Highly similar to a protein of unknown function from Streptomyces coelicolor SC1G2.10 (165 aa), fasta scores opt: 663 z-score: 732.7 E():0 60.1% identity in 163 aa overlap 1 4 7 7 [Reporter]SCO0163 (15M13)_PCR/6185 [Gene]SCO0163/5634 NC_003888 SCJ1.12 unkown, len: 508 aa, similar to Mycobacterium tuberculosis SW:YW13_MYCTU (EMBL; Z74025) hypothetical 54.4 KD protein CY39.13 (498 aa),fasta scores opt: 1192 z-score: 1332.4 E(): 0 43.8% identity in 470 aa overlap and Aquifex aeolicus TR:O66641 (EMBL: AE000682) hypothetical 38.3 KD protein (322 aa), fasta scores opt: 550 z-score: 618.7 E(): 4.2e-27 35.5% identity in 279 aa overlap. Contains a PS00017 ATP/GTP-binding site motif A (P-loop). 1 4 7 6 [Reporter]SCO0266 (16I13)_PCR/6184 [Gene]SCO0266/5633 NC_003888 SCF1.08c, possible transcriptional regulator, len: 325aa; similar to many eg. TR:CAB43482 (EMBL:Y18527) PobR regulator from Pseudomonas sp. strain HR199 (293 aa) fasta scores; opt: 254, z-score: 302.3, E(): 1.8e-09, (30.3% identity in 254 aa overlap). Also similar to TR:O88020 (EMBL:AL031107) putative transcriptional regulator from Streptomyces coelicolor (301 aa) fasta scores; opt: 318, z-score: 376.7, E(): 1.3e-13, (37.2% identity in 253 aa overlap). Contains Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. 1 4 7 5 [Reporter]SCO3671 (17E13)_PCR/6183 [Gene]SCO3671/5632 NC_003888 SCH44.11c, dnaK, heat shock protein 70 (fragment), len: >515 aa; identical to C-terminal region of previously sequenced SW:DNAK_STRCO (EMBL:L46700) Streptomyces coelicolor DnaK protein (heat shock protein 70) 618 aa. Contains Pfam match to entry PF00012 HSP70, Hsp70 protein and matches to Prosite entries PS00329 Heat shock hsp70 proteins family signature 2 and PS01036 Heat shock hsp70 proteins family signature 3. Contains also possible colied-coli region at aprox. residues 388..402,SCH35.53, DnaK, heat shock protein 70, len: >135aa; previously sequenced therefore identical to SW:DNAK_STRCO. Contains Prosite match to PS00297 Heat shock hsp70 proteins family signature 1. 4 2 13 16 [Reporter]SCO6761 (7L7)_PCR/3807 [Gene]SCO6761/3459 NC_003888 SC6A5.10, unknown, len: 52 aa; designated based upon Hidden-Markov Model and probable ribosome binding site. 1 4 7 4 [Reporter]SCO1688 (18A13)_PCR/6182 [Gene]SCO1688/5631 NC_003888 SCI30A.09, putative membrane protein, len: 113 aa; similar to SW:YU38_MYCTU hypothetical protein from Mycobacterium tuberculosis (122 aa) fasta scores; opt: 105, z-score: 141.8, E(): 1.4, (34.2% identity in 120 aa overlap). Contains possible hydrophobic membrane spanning regions 4 2 13 15 [Reporter]SCO4767 (8H7)_PCR/3806 [Gene]SCO4767/3458 NC_003888 SC6G4.45c, probable regulatory protein, len: 112 aa; similar to TR:Q53963 (EMBL:X62287) S.coelicolor regulatory protein WhiB (87 aa), fasta scores; opt: 201 z-score: 352. 1 E(): 2.3e-12, 49.3% identity in 71 aa overlap 1 4 7 3 [Reporter]SCO6111 (19M9)_PCR/6181 [Gene]SCO6111/5630 NC_003888 SCBAC1A6.35c, probable oligopeptide ABC transporter ATP-binding protein, len: 585 aa; similar to SW:YLIA_ECOLI (EMBL:AE000185) Escherichia coli hypothetical ABC transporter ATP-binding protein YliA, 629 aa; fasta scores: opt: 1480 z-score: 1352.7 E(): 0; 44.5% identity in 580 aa overlap and N- and C-terminal regions both similar to SW:OPPD_BACSU (EMBL:X56347) Bacillus subtilis oligopeptide transport ATP-binding protein OppD, 358 aa; fasta scores: opt: 864 z-score: 796.4 E(): 0; 49.6% identity in 274 aa overlap. Contains 2x Pfam matches to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries 2x PS00017 ATP/GTP-binding site motif A (P-loop) and 2x PS00211 ABC transporters family signature 4 2 13 14 [Reporter]SCO7739 (9D7)_PCR/3805 [Gene]SCO7739/3457 NC_003888 SC8D11.30c, possible hydrolase, len: 235 aa; similar to C-terminal region of SW:PCD_ARTOX (EMBL:M94965) Arthrobacter oxidans phemedipham hydrolase (EC 3.1.1.-) Pcd, 493 aa; fasta scores: opt: 219 z-score: 259.2 E(): 6.7e-07; 28.1% identity in 199 aa overlap. Contains 2x Pfam matches to entry PF00135 COesterase, Carboxylesterases 4 2 13 13 [Reporter]SCO6265 (10P3)_PCR/3804 [Gene]SCO6265/3456 NC_003888 SCAH10.30c, scbR, gamma-butyrolactone binding protein, len: 215 aa; identical to previously sequenced TR:O86852 (EMBL:AJ007731) Streptomyces coelicolor gamma-butyrolactone binding protein, 215 aa and to TR:O24741 (EMBL:AB001683) Streptomyces sp. FarA, 221 aa; fasta scores: opt: 771 z-score: 937.4 E(): 0; 57.2% identity in 208 aa overlap. Contains match to Pfam entry PF00440 tetR, Bacterial regulatory proteins, tetR family and to Prosite entry PS01081 Bacterial regulatory proteins, tetR family signature. Contains also a possible helix-turn-helix motif at residues 30..51 (+3.66 SD) 4 2 13 12 [Reporter]SCO2364 (11L3)_PCR/3803 [Gene]SCO2364/3455 NC_003888 SCC8A.22, unknown, len: 331 aa; regions similar to many of undefined function eg. TR:Q9ZBZ1 (EMBL:AL034355) hypothetical protein from Streptomyces coelicolor (482 aa) fasta scores; opt: 686, z-score: 521.2, E(): 1.4e-21, 51.0% identity in 247 aa overlap and SW:P18782 (TERE_ALCSP) tellurium resistance protein from plasmid IncHI2 pMER610 of Alcaligenes sp. (191 aa) fasta scores; opt: 339, z-score: 271.3, E(): 1.2e-07, 35.9% identity in 195 aa overlap 4 2 13 11 [Reporter]SCO4782 (12H3)_PCR/3802 [Gene]SCO4782/3454 NC_003888 SCD63.14c, possible integral membrane protein, len: 219 aa. Contains possible hydrophobic membrane spanning regions 4 2 13 10 [Reporter]SCO3190 (13D3)_PCR/3801 [Gene]SCO3190/3453 NC_003888 SCE22.07, probable mechanosensitive channel, len: 156 aa; similar to TR:Q9Z5G4 (EMBL:AL035500) Mycobacterium leprae putative gated mechanosensitive ion channel MscL, 154 aa; fasta scores: opt: 248 z-score: 308.9 E(): 9.6e-10; 38.1% identity in 147 aa overlap and to SW:MSCL_CLOPE (EMBL:D50309) Clostridium perfringens large-conductance mechanosensitive channel MscL, 152 aa; fasta scores: opt: 216 z-score: 271.1 E(): 1.2e-07; 34.7% identity in 124 aa overlap. Contains Pfam match to entry PF01741 MscL, Large-conductance mechanosensitive channel, MscL and match to Prosite entry PS01327 Large-conductance mechanosensitive channels mscL family signature. Contains also possible hydrophobic membrane spanning regions 3 1 18 17 [Reporter]SCO7778 (6C16)_PCR/1398 [Gene]SCO7778/1279 NC_003888 SC5E9.26, possible transcriptional regulator, len: 255 aa; similar to many e.g. SW:P15360 (GYLR_STRCO) glycerol operon regulatory protein from Streptomyces coelicolor (254 aa) fasta scores; opt: 217, z-score: 260.0, E(): 5.1e-07, 28.1% identity in 249 aa overlap and TR:O88025 (EMBL:AL031107) putative transcriptional regulator from Streptomyces coelicolor (264 aa) fasta scores; opt: 462, z-score: 543.0, E(): 9e-23, 40.5% identity in 242 aa overlap. Contains Pfam match to entry PF01614 IclR, Bacterial transcriptional regulator. 4 2 13 9 [Reporter]SCO0431 (13P23)_PCR/3800 [Gene]SCO0431/3452 NC_003888 SCF51A.09c, possible secreted protein, len: 442 aa; similar to TR:Q9KZR1 (EMBL:AL353832) Streptomyces coelicolor putative secreted protein SCE6.29, 454 aa; fasta scores: opt: 614 Z-score: 655.9 E(): 6.7e-29; 31.278% identity in 454 aa overlap. Contains a possible N-terminal signal sequence 3 1 18 16 [Reporter]SCO5650 (7O12)_PCR/1397 [Gene]SCO5650/1278 NC_003888 SC6A9.17, possible membrane protein, len: 234 aa. Contains possible hydrpphobic membrane spanning regions 4 2 13 8 [Reporter]SCO1787 (14L23)_PCR/3799 [Gene]SCO1787/3451 NC_003888 SCI51.27c, probable iron-siderophore uptake system transmembrane component, len: 348 aa; similar to many transmembrane components from binding-protein-dependent iron uptake systems using a range of siderophores e.g. SW:FEPD_ECOLI (EMBL:X57471), fepD, Escherichia coli ferric enterobactin transport protein (334 aa), fasta scores; opt: 910 z-score: 991.5 E(): 0, 46.0% identity in 326 aa overlap. Similar to neighbouring SCI51.26c, probable iron-siderophore uptake system transmembrane component (375 aa) (37.9% identity in 348 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF01032 FecCD_family, FecCD transport family 3 1 18 15 [Reporter]SCO5756 (8K12)_PCR/1396 [Gene]SCO5756/1277 NC_003888 SC7C7.11, unknown, len: 156 aa; similar to members of the Dps family e.g. FTPA_HAEDU fine tangled pili major subunit (24 KD surface protein) (189 aa), fasta scores; opt: 278 z-score: 370.3 E(): 2.2e-13, 34.4% identity in 151 aa overlap PS_ECOLI DNA protection during starvation protein (166 aa) and, fasta scores; opt: 220 z-score: 261.2 E(): 2.6e-07, 26.8% identity in 153 aa overlap. Contains PS00818 Dps protein family signature 1 4 2 13 7 [Reporter]SCO3497 (15H23)_PCR/3798 [Gene]SCO3497/3450 NC_003888 SCE65.33c, probable polyguluronate lyase precursor, len: 259 aa; highly similar to TR:BAA83339 (EMBL:AB030481) Corynebacterium sp. ALY-1 polyguluronate lyase precursor (EC 4.2.2.11) AlyPG, 256 aa; fasta scores: opt: 1329 z-score: 1518.4 E(): 0; 76.9% identity in 260 aa overlap. Contains possible N-terminal signal peptide sequence 3 1 18 14 [Reporter]SCO2636 (9G12)_PCR/1395 [Gene]SCO2636/1276 NC_003888 SC8E4A.06, unknown, len: 396 aa 3 1 18 13 [Reporter]SCO6240 (10C12)_PCR/1394 [Gene]SCO6240/1275 NC_003888 SCAH10.05c, hypothetical protein, len: 244 aa; similar to various hypothetical proteins, e.g. TR:CAB52958 (EMBL:AL109950) Streptomyces coelicolor hypothetical 25.2 KD protein SCJ4.24c, len: 242 aa; fasta scores: opt: 504 z-score: 580.0 E(): 6.2e-25; 36.9% identity in 241 aa overlap 3 1 18 12 [Reporter]SCO4400 (11O8)_PCR/1393 [Gene]SCO4400/1274 NC_003888 SCD10.32, possible membrane protein, len: 531 aa. Contains possible hydrophobic membrane spanning region and high content in alanine, glycine and proline amino acid residues. Also high G+C content (79.03%) and a 3x degenerate repeat unit: GGR 3 1 18 11 [Reporter]SCO4636 (12K8)_PCR/1392 [Gene]SCO4636/1273 NC_003888 SCD82.07, hypothetical protein, len: 150 aa; similar to SW:YV31_MYCTU (EMBL:Z77162) Mycobacterium tuberculosis hypothetical 18.4 kD protein CY20G9.31c, 166 aa; fasta scores: opt: 304 z-score: 373.0 E(): 2.4e-13; 35.0% identity in 143 aa overlap. Contains TTA leucine codon, possible target for bldA regulation 3 1 18 10 [Reporter]SCO3236 (13G8)_PCR/1391 [Gene]SCO3236/1272 NC_003888 SCE29.05c, possible oxygenase, len: 333 aa; similar to TR:Q05581 (EMBL:L06213) clavaminate synthase I, an alpha-ketoglutarate-dependent oxygenase central to the biosynthesis of clavulanic acid, a potent inhibitor of beta-lactamases (324 aa) fasta scores; opt: 498, z-score: 571.8, E(): 1.5e-24, (34.3% identity in 283 aa overlap). 3 1 18 8 [Reporter]SCO3805 (15O4)_PCR/1389 [Gene]SCO3805/1271 NC_003888 SCGD3.06, hypothetical protein, len: 164 aa; unknown function, weakly similar to TR:P94352 (EMBL:D83026) Bacillus subtilis hypothetical protein (162 aa), fasta scores; opt: 185 z-score: 240.2 E(): 4.7e-06, 21.8% identity in 165 aa overlap. An alternative start codon is present at codon 5 3 1 18 7 [Reporter]SCO1622 (16K4)_PCR/1388 [Gene]SCO1622/1270 NC_003888 SCI41.05, probable 5'-3' exonuclease, len: 301 aa. High similarity to SW:EX53_MYCTU (EMBL:Z73966) Mycobacterium tuberculosis potential 5'-3' exonuclease (EC 3.1.11.-), 393 aa; fasta scores: opt: 964 z-score: 1025.6 E(): 0; 52.3% identity in 308 aa overlap. Contains Pfam match to entry PF01367 5_3_exonuclease, 5'-3' exonuclease. 1 4 7 2 [Reporter]SCO6082 (20E9)_PCR/6180 [Gene]SCO6082/5629 NC_003888 SCBAC1A6.06c, glgX3, glycogen debranching enzyme, len: 702 aa; similar to SW:GLGX_ECOLI (EMBL:J01616) Escherichia coli glycogen operon protein GlgX or GlyX (EC 3.2.1.*), 657 aa; fasta scores: opt: 1883 z-score: 2219.2 E(): 0; 45.5% identity in 664 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase 1 4 6 22 [Reporter]SCO5381 (1E5)_PCR/6178 [Gene]SCO5381/5628 NC_003888 2SC6G5.25c, conserved hypothetical protein, len: 190aa; similar to many eg. SW:Q10622 (Y02Y_MYCTU) hypothetical protein from Mycobacterium tuberculosis (193 aa) fasta scores; opt: 800, z-score: 984.0, E(): 0, 65.6% identity in 189 aa overlap. Contains Pfam match to entry PF01923 DUF80, Protein of unknown function. 1 4 6 21 [Reporter]SCO0778 (2A5)_PCR/6177 [Gene]SCO0778/5627 NC_003888 3SCF60.10, possible integral membrane protein, len: 102 aa. Contains possible hydrophobic membrane spanning regions 1 4 6 20 [Reporter]SCO1513 (3M1)_PCR/6176 [Gene]SCO1513/5626 NC_003888 SCL2.03c, relA, GTP pyrophosphokinase, len: 847 aa; identical to previously sequenced SW:RELA_STRCO (EMBL:X87267) Streptomyces coelicolor GTP pyrophosphokinase (EC 2.7.6.5) RelA, 847 aa and highly similar to SW:RELA_STRAT (EMBL:AF072829) Streptomyces antibioticus RelA protein, 841 aa; fasta scores: opt: 4969 z-score: 4906.2 E(): 0; 90.3% identity in 848 aa overlap and to SW:RELA_CORGL (EMBL:AF038651) Corynebacterium glutamicum GTP pyrophosphokinase (EC 2.7.6.5) RelA, 760 aa; fasta scores: opt: 1613 z-score: 1594.3 E(): 0; 58.7% identity in 774 aa overlap. Contains Pfam match to entry PF01842 ACT, ACT domain and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 4 6 19 [Reporter]SCO6032 (4I1)_PCR/6175 [Gene]SCO6032/5625 NC_003888 SC1C3.20c, possible hydrolase, len: 506 aa; weakly similar to many e.g. HEXA_ALTSO beta-hexosaminidase a precursor (598 aa), fasta scores; opt: 463 z-score: 613.4 E(): 6.2e-27, 29.7% identity in 526 aa overlap 1 4 6 18 [Reporter]SCO7097 (5E1)_PCR/6174 [Gene]SCO7097/5624 NC_003888 SC3A4.23c, possible MarR-family regulator protein, len: 196 aa. Similar to several including: Erwinia chrysanthemi SW:PECS_ERWCH (EMBL:X74409) regulatory protein, PecS (166 aa), fasta scores opt: 359 z-score: 425.8 E(): 2.9e-16 38.8% identity in 170 aa overlap and Streptomyces coelicolor TR:CAB71822 (EMBL:AL138662) putative MarR-family regulatory protein, SC8E4A.17 (172 aa), fasta scores opt: 359 z-score: 425.8 E(): 2.9e-16 38.8% identity in 170 aa overlap. Contains a Pfam match to entry PF01047 MarR, MarR family. 1 4 6 17 [Reporter]SCO7328 (6A1)_PCR/6173 [Gene]SCO7328/5623 NC_003888 SC4G10.07c, possible regulatory protein, len: 144 aa. Highly similar to Streptomyces fradiae TR:Q9Z9I5 (EMBL:AB024056) beta-lactamase regulator protein (139 aa), fasta scores opt: 530 z-score: 661.7 E(): 2.2e-29 61.5% identity in 122 aa overlap and Streptomyces coelicolor TR:Q9X7S3 (EMBL:AL049863) putative regulator protein, SC5H1.15C (151 aa), fasta scores opt: 178 z-score: 231.0 E(): 2.1e-05 30.8% identity in 130 aa overlap. 1 4 6 16 [Reporter]SCO7316 (6M21)_PCR/6172 [Gene]SCO7316/5622 NC_003888 SC5F8.26, possible integral membrane protein, len: 280 aa. Similar to Bacillus subtilis SW:YXXB_BACSU(EMBL:D45912) hypothetical 31.8 kd protein (289 aa), fasta scores opt: 476 z-score: 539.7 E(): 1.4e-22 31.0% identity in 245 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 4 2 13 6 [Reporter]SCO4621 (16D23)_PCR/3797 [Gene]SCO4621/3449 NC_003888 SCD39.21c, traA1, possible sporulation-related protein, len: 179 aa; highly similar to TR:Q54678 (EMBL:U23762) Streptomyces phaeochromogenes involved in intermycelial plasmid transfer TraA, 184 aa; fasta scores: opt: 472 z-score: 523.1 E(): 1.1e-21; 50.0% identity in 172 aa overlap 1 4 6 15 [Reporter]SCO6952 (7I21)_PCR/6171 [Gene]SCO6952/5621 NC_003888 SC6F7.05c, unknown, len: 347 aa. Weakly similar to several proteins of unknown function e.g. Mycobacterium tuberculosis SW:Y07U_MYCTU(EMBL:Z75555) (273 aa), fasta scores opt: 173 z-score: 212.9 E(): 0.0002 26.8% identity in 276 aa overlap. 4 2 13 4 [Reporter]SCO4051 (18L19)_PCR/3795 [Gene]SCO4051/3448 NC_003888 2SCD60.17, conserved hypothetical protein, len: 165 aa; similar to TR:P72845 (EMBL:D90901) Synechocystis sp. hypothetical 18.8 kDa protein SLL1188, 164 aa; fasta scores: opt: 339 z-score: 385.2 E(): 6.8e-14; 38.3% identity in 154 aa overlap 1 4 6 14 [Reporter]SCO6889 (8E21)_PCR/6170 [Gene]SCO6889/5620 NC_003888 SC7F9.41, unknown, len: 246 aa. Similar to Streptomyces coelicolor TR:Q9X8A7 (EMBL:AL049645) hypothetical 28.5 KD protein, SCE2.12 (259 aa), fasta scores opt: 405 z-score: 450.6 E(): 1.1e-17 35.6% identity in 233 aa overlap. 4 2 13 3 [Reporter]SCP1.18c (19H19)_PCR/3794 [Gene]SCP1.18c/3447 NC_003888 SCP1.18c, possible DNA integrase/recombinase, len: 783aa; C-terminal region weakly similar to many members of the 'phage' integrase family eg. SW:P06697 (TNPB_STAAU) transposase B (transposon Tn554) from Staphylococcus aureus (630 aa) fasta scores; opt: 205, z-score: 242.4, E(): 4.9e-06, 22.1% identity in 280 aa overlap. Contains Pfam match to entry PF00589 Phage_integrase, Phage integrase family. 4 2 13 2 [Reporter]SCO3047 (20P15)_PCR/3793 [Gene]SCO3047/3446 NC_003888 SCBAC19G2.02, possible membrane protein, len: 524aa: similar to many eg. neighbouring gene TR:Q9KZK3 (EMBL:AL353862) SCE34.23 putative transcriptional regulator (396 aa) fasta scores; opt: 631, Z-score: 610.7, 36.011% identity (38.235% ungapped) in 361 aa overlap and TR:O68907 (EMBL:AF058302) FrnA from polyketide antibiotic frenolicin biosynthetic cluster of Streptomyces roseofulvus (770 aa) fasta scores; opt: 482, Z-score: 464.5, 28.387% identity (32.195% ungapped) in 465 aa overlap. Contains possible membrane-spanning hydrophobic region. Also has region of similarity with adjacent CDS SCBAC19F3.03c. 4 2 12 22 [Reporter]SCO1268 (1P11)_PCR/3791 [Gene]SCO1268/3445 NC_003888 2SCG18.15c, probable acyltransferase, len: 372 aa; similar to SW:ODO2_ECOLI (EMBL:J01619) Escherichia coli dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61) SucB, 404 aa; fasta scores: opt: 281 z-score: 311.6 E(): 7.1e-10; 24.8% identity in 400 aa overlap. Contains Pfam match to entry PF00364 biotin_lipoyl, Biotin-requiring enzymes and match to Prosite entry PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site 4 2 12 21 [Reporter]SCO7638 (2L11)_PCR/3790 [Gene]SCO7638/3444 NC_003888 SC10F4.11c, eno2, enolase, len: 434 aa; highly similar to SW:ENO_ECOLI (EMBL:X82400) Escherichia coli enolase (EC 4.2.1.11) Eno, 431 aa; fasta scores: opt: 1537 z-score: 1648.0 E(): 0; 57.5% identity in 426 aa overlap and to TR:CAC09537 (EMBL:AL442120) Streptomyces coelicolor eno, enolase SCE41.05c, 426 aa; fasta scores: opt: 1594 z-score: 1484.2 E(): 0; 58.2% identity in 423 aa overlap. Contains Pfam match to entry PF00113 enolase, Enol-ase and match to Prosite entry PS00164 Enolase signature 4 2 12 20 [Reporter]SCO7236 (3H11)_PCR/3789 [Gene]SCO7236/3443 NC_003888 SC7A12.03c, qcrB3, ubiquinol-cytochrome C reductase cytochrome subunit B, len: 554 aa. Highly similar to many including: Streptomyces lividans SW:QCRB_STRLI(EMBL:AF107888) ubiquinol-cytochrome C reductase cytochrome subunit B (549 aa), fasta scores opt: 1808 z-score: 1914.0 E():0 51.9% identity in 528 aa overlap and Streptomyces coelicolor SW:QCRB_STRCO (EMBL:AL049497) ubiquinol-cytochrome C reductase cytochrome subunit B, QcrB OR SC6G10.21c (545 aa), opt: 2016 z-score: 2133.7 E():0 55.0% identity in 522 aa overlap. Contains a Pfam match to entry PF00033 cytochrome_b_N, Cytochrome b(N-terminal)/b6/petB and multiple possible membrane spanning hydrophobic domains. 3 1 18 6 [Reporter]SCO0166 (17G4)_PCR/1387 [Gene]SCO0166/1269 NC_003888 SCJ1.15, possible regulator, len: 267 aa, highly similar to Pseudomonas aeruginosa substrain OT684 TR:P72119 (EMBL:U12891) pyoverdine gene transcriptional regulator PvdS (237 aa), fasta scores opt: 1040 z-score: 1209.8 E(): 0 60.4% identity in 235 aa overlap and Mycobacterium tuberculosis TR:O05877 (EMBL; Z95121) hypothetical 34.1 KD protein MTCY20B11.07C (295 aa) fasta scores opt: 1189 z-score: 1380.2 E(): 0 65.6% identity in 262 aa overlap. 4 2 12 19 [Reporter]SCO6018 (4D11)_PCR/3788 [Gene]SCO6018/3442 NC_003888 SC1C3.06, unknown, len: 341 aa; similar to downsteam CDS SC1C3.07 (E(): 2.3e-19, 36.2% identity in 348 aa overlap, and to C-terminus of SC1C3.13 (E(): 3.7e-09, 32.0% identity in 356 aa overlap) 3 1 18 4 [Reporter]SCP1.279c (18O24)_PCR/1385 [Gene]SCP1.279c/1268 NC_003888 SCP1.279c, unknown, len: 361aa; 4 2 12 17 [Reporter]SCO5831 (6L7)_PCR/3786 [Gene]SCO5831/3441 NC_003888 SC5B8.21c, citrate synthase-like protein, len: 421 a a; similar to citrate synthase from many organisms e.g. CIS Y_THIFE P51045 thiobacillus ferrooxidans. citrate synthase (386 aa), fasta scores; opt: 387 z-score: 460.4 E(): 1.9e-1 8, 30.2% identity in 384 aa overlap. Contains Pfam match to entry citrate_synt PF00285, Citrate synthase, score 81.00 and probable helix-turn-helix at aa 17-38 (Score 1757, +5.1 7 SD). Also similar to upstream gene SC5B8.22 (390 aa) E(): 4.5e-11, 32.8% identity in 351 aa overlap. 3 1 18 3 [Reporter]SCO4001 (19K24)_PCR/1384 [Gene]SCO4001/1267 NC_003888 2SC10A7.05c, unknown, len: 291 aa 4 2 12 16 [Reporter]SCO7261 (7H7)_PCR/3785 [Gene]SCO7261/3440 NC_003888 SC5H1.31, possible membrane protein, len: 147 aa; unknown function, similar to TR:O07432 (EMBL:Z97050) Mycobacterium tuberculosis hypothetical protein (143 aa), fasta scores; opt: 326 z-score: 414.4 E(): 9.2e-16, 36.2% identity in 141 aa overlap 3 1 18 2 [Reporter]SCO3979 (20C24)_PCR/1383 [Gene]SCO3979/1266 NC_003888 SCBAC25E3.16, possible TetR-family transcriptional regulator, len: 196 aa: similar to many from Streptomyces coelicolor e.g. TR:Q9RK21 (EMBL:AL109974) putative TetR-family transcriptional regulator SCF34.04 (203 aa) fasta scores; opt: 497, Z-score: 587.5, 43.750% identity (44.681% ungapped) in 192 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. 3 1 17 22 [Reporter]SCO1129 (1C20)_PCR/1381 [Gene]SCO1129/1265 NC_003888 2SCG38.22, probable secreted deacetylase, len: 282 aa; similar to SW:NODB_RHIME (EMBL:X01649) Rhizobium meliloti chitooligosaccharide deacetylase (EC 3.5.1.-) NodB, 217 aa; fasta scores: opt: 363 z-score: 383.5 E(): 6.5e-14; 38.5% identity in 195 aa overlap. Contains Pfam match to entry PF01522 Polysac_deacet, Polysaccharide deacetylase and possible N-terminal region signal peptide sequence 3 1 17 21 [Reporter]SCO6234 (2O16)_PCR/1380 [Gene]SCO6234/1264 NC_003888 SC2H4.16, manA, probable secreted beta-mannosidase, len: 384 aa; 94.5% identical to MANA_STRLI mannan endo-1,4-beta-mannosidase precursor (363 aa). Contains PS00659 Glycosyl hydrolases family 5 signature and Pfam match to entry PF00150 cellulase, Cellulase (glycosyl hydrolase family 5), score 104.70, E-value 1.8e-27 3 1 17 20 [Reporter]SCO6823 (3K16)_PCR/1379 [Gene]SCO6823/1263 NC_003888 SC1A2.32, possible ArsR-family transcriptional regulator, len: 150 aa. Similar to several including: Sinorhizobium sp. As4 TR:AAD51845 (EMBL:AF178758) ArsR regulatory protein (115 aa), fasta scores opt: 129 z-score: 168.5 E(): 0.056 29.1% identity in 103 aa overlap. Contains a Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family with a putative helix-turn-helix motif situated between residues 68..89 (+3.74 SD). 3 1 17 19 [Reporter]SCO6273 (4G16)_PCR/1378 [Gene]SCO6273/1262 NC_003888 SC2C4.03c, probable type I polyketide synthase, len: 2152 aa; N-terminal domain almost identical to TR:Q9L8J0 (EMBL:AF202898) Streptomyces coelicolor A3(2) type I polyketide synthase (fragment), 720 aa; fasta scores: opt: 4356 z-score: 4494.6 E(): 0; 93.8% identity in 721 aa overlap and C-terminal domain similar to SW:HETM_ANASP (EMBL:L22883) Anabaena sp. polyketide synthase HetM, 506 aa; blastp scores: Score= 761 (267.9 bits), Expect= 2.2e-74, Sum P(2)= 2.2e-74; Identities= 184/491 (37%), Positives= 278/491 (56%). Also highly similar, in this same cosmid, to the N-terminal region of SC2C4.04c and to SC2C4.05c. Contains Pfam matches to entries PF00109 ketoacyl-synt, Beta-ketoacyl synthase, PF00698 Acyl_transf, Acyl transferase domain and PF00550 pp-binding, Phosphopantetheine attachment site and matches to Prosite entries PS00606 Beta-ketoacyl synthases active site, PS00340 Growth factor and cytokines receptors family signature 2 and PS00012 Phosphopantetheine attachment site 3 1 17 18 [Reporter]SCO2397 (5C16)_PCR/1377 [Gene]SCO2397/1261 NC_003888 SC4A7.25c, probable oxidoreductase, len: 364 aa; similar to SW:YNCB_ECOLI (EMBL:AE000242) Escherichia coli putative NADP-dependent oxidoreductase in TehB-RhsE intergenic region YncB, 353 aa; fasta scores: opt: 950 z-score: 1018.4 E(): 0; 43.6% identity in 335 aa overlap and to SW:P2_ARATH (EMBL:Z49268) Arabidopsis thaliana probable NADP-dependent oxidoreductase P2, 342 aa; fasta scores: opt: 880 z-score: 944.2 E(): 0; 45.4% identity in 328 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases 3 1 17 17 [Reporter]SCO6544 (6O12)_PCR/1376 [Gene]SCO6544/1260 NC_003888 SC5C7.29, possible membrane protein, len: 226 aa; contains possible transmembrane domain around aa 40 1 4 6 13 [Reporter]SCO7167 (9A21)_PCR/6169 [Gene]SCO7167/5619 NC_003888 SC9A4.29c, probable secreted sugar-binding protein, len: 425 aa; similar to TR:Q9RJV8 (EMBL:AL117387) Streptomyces coelicolor putative solute-binding protein SCF41.11, 422 aa; fasta scores: opt: 946 z-score: 1081.0 E(): 0; 40.1% identity in 419 aa overlap and to SW:MSME_STRMU (EMBL:M77351) Streptococcus mutans multiple sugar-binding protein precursor MsmE, 420 aa; fasta scores: opt: 233 z-score: 268.9 E(): 1.6e-07; 25.3% identity in 297 aa overlap. Contains 2x Pfam matches to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 1 4 6 12 [Reporter]SCO1953 (10M17)_PCR/6168 [Gene]SCO1953/5618 NC_003888 SCC54.13c, uvrC, ABC excision nuclease subunit C, len: 728aa; member of a conserved family eg, SW:UVRC_MYCTU uvrC, excinuclease ABC subunit C from Mycobacterium tuberculosis (646 aa) fasta scores; opt: 1516, z-score: 1657.1, E(): 0, (58.4% identity in 693 aa overlap) and SW:UVRC_BACSU uvrC, excinuclease ABC subunit C from Bacillus subtilis (598 aa) fasta scores; opt: 567, z-score: 619.9, E(): 3.2e-27, (34.0% identity in 653 aa overlap). 1 4 6 11 [Reporter]SCO4521 (11I17)_PCR/6167 [Gene]SCO4521/5617 NC_003888 SCD35.28c, possible secreted protein, len: 361 aa; similar to TR:Q9F3F6 (EMBL:AL392175) Streptomyces coelicolor putative secreted protein 2SCD4.18, 355 aa; fasta scores: opt: 942 Z-score: 1049.8 E(): 7.7e-51; 43.296% identity. Contains possible N-terminal region signal peptide sequence 1 4 6 10 [Reporter]SCO4279 (12E17)_PCR/6166 [Gene]SCO4279/5616 NC_003888 SCD95A.12c, possible acetyltransferase, len: 156 aa; similar to TR:AAF22297 (EMBL:AF185569) Rattus norvegicus putative N-acetyltransferase camello 2, Cml2, 221 aa; fasta scores: opt: 125 z-score: 168.1 E(): 0.067 23.6% identity in 123 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 4 6 9 [Reporter]SCO3856 (13A17)_PCR/6165 [Gene]SCO3856/5615 NC_003888 SCH69.26c, probable peptidyl-prolyl cis-trans isomerase, len: 177aa; similar to many egs. TR:P77949 (EMBL:U64692) peptidyl-prolyl cis-trans isomerase from Streptomyces chrysomallus (175 aa) fasta scores; opt: 950, z-score: 1136.4, E(): 0, (78.3% identity in 175 aa overlap) and TR:O54168 (EMBL:AL021411) peptidyl-prolyl isomerase from Streptomyces coelicolor (157 aa) fasta scores; opt: 400, z-score: 486.8, E(): 8.8e-20, (50.0% identity in 124 aa overlap). Contains Pfam match to entry PF00160 pro_isomerase, Peptidyl-prolyl cis-trans isomerase. 1 4 6 8 [Reporter]SCO1569 (14M13)_PCR/6164 [Gene]SCO1569/5614 NC_003888 SCL24.05c, possible oxidoreductase, len: 350 aa; similar to TR:AAF09836 (EMBL:AE001886) Deinococcus radiodurans oxidoreductase, putative DR0249, 357 aa; fasta scores: opt: 1019 z-score: 1133.1 E(): 0; 51.0% identity in 359 aa overlap 1 4 6 7 [Reporter]SCO3485 (15I13)_PCR/6163 [Gene]SCO3485/5613 NC_003888 SCE65.21c, probable lacI-family transcriptional regulator, len: 349 aa; similar to TR:AAD42741 (EMBL:AF105219) Thermomonospora curvata putative regulatory protein AglR, 347 aa; fasta scores: opt: 563 z-score: 648.1 E(): 1e-28; 34.9% identity in 341 aa overlap and to SW:CYTR_ECOLI (EMBL:X03683) Escherichia coli, transcriptional repressor CytR 341 aa; fasta scores: opt: 552 z-score: 635.6 E(): 5e-28; 30.5% identity in 347 aa overlap. Contains matches to Pfam entries PF00356 lacI, Bacterial regulatory proteins, lacI family PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI. Contains probable helix-turn-helix motif at residues 18..39 (+4.84 SD) 1 4 6 6 [Reporter]SCO0257 (16E13)_PCR/6162 [Gene]SCO0257/5612 NC_003888 SCF20.03, hypothetical protein, len: 333 aa; similar to TR:Q9Y9P8 (EMBL:AP000063) hypothetical protein APE2240 from Aeropyrum pernix (324 aa) fasta scores; opt: 728, z-score: 908.5, E(): 0, (51.7% identity in 298 aa overlap) 4 2 12 15 [Reporter]SCO4763 (8D7)_PCR/3784 [Gene]SCO4763/3439 NC_003888 SC6G4.41c, unknown, len: 413 aa 1 4 6 5 [Reporter]SCO0065 (17A13)_PCR/6161 [Gene]SCO0065/5611 NC_003888 SCJ4.46, possible extracellular binding protein, part of a possible binding protein dependent transport system, len: 437 aa; similar to many egs. TR:Q9Z492 (EMBL:AF043654) BxlE, extracellular binding protein from Streptomyces lividans (434 aa) fasta scores; opt: 256, z-score: 290.5, E(): 8.1e-09, (23.3% identity in 446 aa overlap) and SW:YC14_PYRHO hypothetical ABC transporter extracellular binding protein from Pyrococcus horikoshii OT3 (441 aa) fasta scores; opt: 348, z-score: 393.3, E(): 1.5e-14, (25.4% identity in 343 aa overlap). Contains Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site so may be anchored by lipid. 4 2 12 14 [Reporter]SCO7811 (9P3)_PCR/3783 [Gene]SCO7811/3438 NC_003888 SC8E7.08c, possible membrane protein, len: 126 aa. Weakly similar to several e.g. Streptomyces coelicolor TR:Q9RJS0(EMBL:AL132707) probable integral membrane protein, SCF51.15C (201 aa), fasta scores opt: 106 z-score: 142.5 E():1.8 33.7% identity in 104 aa overlap. Contains possible membrane spanning hydrophobic domains. 1 4 6 4 [Reporter]SCO3413 (18M9)_PCR/6160 [Gene]SCO3413/5610 NC_003888 SCE9.20c, tipA, transcriptional regulator, len: 253 aa; highly similar to many e.g. SW:TIPA_STRLI (EMBL:S64314), TipA, Streptomyces lividans thiostrepton-binding transcriptional activator (253 aa), fasta scores; opt: 1693 z-score: 1909.7 E(): 0, 99.6% identity in 253 aa overlap. Weakly similar to others from S. coelicolor e.g. TR:O54191 (EMBL:AL021411) probable transcriptional regulator (196 aa) (29.8% identity in 181 aa overlap). Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family, score 66.50, E-value 5.7e-1 and PS00552 Bacterial regulatory proteins, merR family signature. Contains probable coiled-coil from 77 to 107 (31 residues) (Max score: 1.523, probability 0.97) 4 2 12 13 [Reporter]SCO6268 (10L3)_PCR/3782 [Gene]SCO6268/3437 NC_003888 SCAH10.33c, possible histidine kinase orfB, len: 254 aa; identical to previously sequenced TR:O86849 (EMBL:AJ007731) Streptomyces coelicolor putative histidine kinase ORFB, 310 aa and to SW:UHPB_SALTY (EMBL:M89480) Salmonella typhimurium sensor protein UhpB, 500 aa; fasta scores: opt: 219 z-score: 254.8 E(): 8e-07; 33.3% identity in 204 aa overlap. Contains a match to Prosite entry PS00136 Serine proteases, subtilase family, aspartic acid active site 4 2 12 12 [Reporter]SCO2265 (11H3)_PCR/3781 [Gene]SCO2265/3436 NC_003888 SCC75A.11c, unknown, len: 92 aa. 4 2 12 10 [Reporter]SCO2840 (12P23)_PCR/3779 [Gene]SCO2840/3435 NC_003888 SCE20.14, possible LysR-family transcriptional regulatory protein, len: 297 aa. Similar to many other regulators including: Bacillus subtilis SW:ALSR_BACSU (EMBL:L04470;) als operon regulatory protein (302 aa). fasta scores opt: 373 z-score: 437.5 E(): 5.5e-17 26.6% identity in 286 aa overlap and Streptomyces lividans TR:Q9X9S5 (EMBL:Y18818) transcriptional regulator of LysR family (307 aa), fasta scores opt: 449 z-score: 525.1 E(): 7.3e-22 34.0% identity in 303 aa overlap. Contains a Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family with a putative helix-turn-helix motif situated between residues 17..38 (+4.47 SD). 4 2 12 9 [Reporter]SCO0585 (13L23)_PCR/3778 [Gene]SCO0585/3434 NC_003888 SCF55.09c, cvnD11, probable ATP/GTP binding protein, len: 178 aa; similar to TR:CAB41559 (EMBL:AL049727) Streptomyces coelicolor putative ATP/GTP binding protein, SC9B1.13c, 181 aa; fasta scores: opt: 617 z-score: 780.0 E(): 0; 57.5% identity in 179 aa overlap and low similarity to SW:YPT1_VOLCA (EMBL:M93438) Volvox carteri GTP binding protein YPTV1, 203 aa; fasta scores: opt: 117 z-score: 156.9 E(): 0.23; 23.2% identity in 138 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 2 12 8 [Reporter]SCO3663 (14H23)_PCR/3777 [Gene]SCO3663/3433 NC_003888 SCH44.03c, possible membrane protein, len: 63 aa. Contains possible hydrophobic membrane spanning regions 3 1 17 16 [Reporter]SCO5654 (7K12)_PCR/1375 [Gene]SCO5654/1259 NC_003888 SC6A9.13, probable ABC transporter ATP-binding protein, len: 258 aa; similar to many e.g. YBBA_ECOLI hypothetical ABC transporter ATP-binding protein (228 aa), fasta scores; opt: 462 z-score: 578.7 E(): 5.3e-25, 40.4% identity in 198 aa overlap. Contains PS00017 ATP /GTP-binding= PF00005 ABC_tran, ABC transporters, score 173.20, E-value 4.2e-48 4 2 12 7 [Reporter]SCO4974 (15D23)_PCR/3776 [Gene]SCO4974/3432 NC_003888 2SCK31.34, possible deaminase, len: 167 aa; similar to TR:AAG05887 (EMBL:AE004677) Pseudomonas aeruginosa probable deaminase PA2499, 151 aa; fasta scores: opt: 299 z-score: 372.9 E(): 3.1e-13; 36.6% identity in 123 aa overlap. Contains Pfam match to entry PF00383 dCMP_cyt_deam, Cytidine and deoxycytidylate deaminase zinc-binding region 3 1 17 15 [Reporter]SCO5289 (8G12)_PCR/1374 [Gene]SCO5289/1258 NC_003888 SCCB12.13, cvnA5, hypothetical protein (fragment), len: >191 aa; similar to C-terminal region of TR:O86525 (EMBL:AL031124) Streptomyces coelicolor hypothetical 139.7 kDa protein SC1C2.25c, 1329 aa; fasta scores: opt: 146 z-score: 152.5 E(): 0.5; 25.3% identity in 186 aa overlap. Contains possible hydrophobic membrane spanning region,SC6G9.44c, possible sensor kinase, len: 806 aa; weak similarity to part of TR:O33492 (EMBL:AJ000330), StyS, Pseudomonas sp. strain Y2 styrene sensor kinase (982 aa), fasta scores; opt: 256 z-score: 230.5 E(): 1.6e-05, 24.7% identity in 543 aa overlap. Similar to parts of other S.coelicolor hypothetical proteins e.g. TR:O86525 (EMBL:AL031124) S.coelicolor hypothetical protein (1329 aa) (31.5% identity in 821 aa overlap). Contains 11x degenerate E(K/T)A repeats between codons 555 and 587. Contains probable coiled-coil from 251 to 295 (45 residues) (Max score: 1.500, probability 0.95). Contains Pfam match to entry PF00672 DUF5, Domain found in bacterial signal proteins and to entry PF00512 signal, Signal carboxyl-terminal domain 4 2 12 6 [Reporter]SCO0230 (16P19)_PCR/3775 [Gene]SCO0230/3431 NC_003888 SCJ9A.09, possible tetR-family transcriptional regulator, len: 204 aa; similar to many proposed regulators and hypothetical proteins e.g. TR:CAB46789 (EMBL:AL096811) putative tetR transcriptional regulator from Streptomyces coelicolor (251 aa) fasta scores; opt: 194, z-score: 252.1, E(): 1.1e-06, (35.3% identity in 102 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and Prosite match to PS01081 Bacterial regulatory proteins, tetR family signature. Also contains possible helix-turn-helix motif (+3.50 SD) 41-62 aa 3 1 17 14 [Reporter]SCO2629 (9C12)_PCR/1373 [Gene]SCO2629/1257 NC_003888 SC8E4.04, putative membrane protein, len: 48 aa. Contains possible hydrophobic membrane spanning region 4 2 12 5 [Reporter]SCO1606 (17L19)_PCR/3774 [Gene]SCO1606/3430 NC_003888 SCI35.28, possible membrane protein, len: 322 aa; similar to S. coelicolor TR:O69914 (EMBL:AL023861) putative secreted protein SC3C8.01 (302 aa), fasta scores; opt: 730 z-score: 709.0 E(): 3e-32, 48.0% identity in 275 aa overlap. Contains possible hydrophobic membrane spanning region 3 1 17 13 [Reporter]SCO2520 (10O8)_PCR/1372 [Gene]SCO2520/1256 NC_003888 SCC121.23c, possible membrane protein, len: 377 aa. Contains possible hydrophobic membrane spanning regions 3 1 17 12 [Reporter]SCO4396 (11K8)_PCR/1371 [Gene]SCO4396/1255 NC_003888 SCD10.28c, possible integral membrane transport protein, len: 550 aa; similar to SW:MER4_STRLI (EMBL:X65467) Streptomyces lividans mercury resistance probable Hg transport protein Mer4, 319 aa; fasta scores: opt: 139 z-score: 153.9 E(): 0.43; 27.9% identity in 308 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 17 11 [Reporter]SCO4471 (12G8)_PCR/1370 [Gene]SCO4471/1254 NC_003888 SCD65.14, possible secreted protein, len: 432 aa; similar to TR:Q9X926 (EMBL:AL035636) Streptomyces coelicolor putative secreted protein SCH5.24c, 431 aa; fasta scores: opt: 1505 z-score: 1459.2 E(): 0; 50.0% identity in 438 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 3 1 17 10 [Reporter]SCO3091 (13C8)_PCR/1369 [Gene]SCO3091/1253 NC_003888 SCE25.32c, cyclopropane-fatty-acyl-phospholipid synthase, len: 438 aa; similar to TR:AAF1173 (EMBL:AE002051) Deinococcus radiodurans cyclopropane-fatty-acyl-phospholipid synthase, putative DR2187, 462 aa; fasta scores: opt: 832 z-score: 910.3 E(): 0; 42.7% identity in 433 aa overlap and to SW:CFA_ECOLI (EMBL:M98330) Escherichia coli cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79) (cyclopropane fatty acid synthase) (CFA synthase) Cfa or CdfA, 381 aa; fasta scores: opt: 482 z-score: 530.2 E(): 4.5e-22; 38.0% identity in 303 aa overlap 3 1 17 9 [Reporter]SCO3784 (14O4)_PCR/1368 [Gene]SCO3784/1252 NC_003888 SCH63.31c, unknown, len: 359 aa. High content in alanine, leucine and arginine amino acid residues. Also high content in G+C (79.48%) 3 1 17 8 [Reporter]SCO0486 (15K4)_PCR/1367 [Gene]SCO0486/1251 NC_003888 SCF34.05c, possible monooxygenase, len: 407 aa; similar to TR:Q9ZI64 (EMBL:AF039534), NahW, Pseudomonas stutzeri salicylate hydroxylase (389 aa), fasta scores; opt: 328 z-score: 383.8 E(): 5.1e-14, 27.0% identity in 403 aa overlap and to the N-terminal half of some epoxidases e.g. SW:ABA2_LYCES (EMBL:Z83835) Lycopersicon esculentum zeaxanthin epoxidase precursor (669 aa) (26.8% identity in 336 aa overlap). Also similar to many putative oxidoreductases e.g. TR:O54177 (EMBL:AL021411) Streptomyces coelicolor possible oxidoreductase (397 aa) (56.1% identity in 403 aa overlap). Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase 3 1 17 7 [Reporter]SCO1692 (16G4)_PCR/1366 [Gene]SCO1692/1250 NC_003888 SCI30A.13c, possible oxidoreductase, len: 256 aa; similar to many e.g. SW:FABG_ECOLI 3-oxoacyl-(carrier protein) reductase from Escherichia coli (244 aa) fasta scores; opt: 239, z-score: 270.9, E(): 9.2e-08, (27.3% identity in 198 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature. 1 4 6 3 [Reporter]SCO6292 (19I9)_PCR/6159 [Gene]SCO6292/5609 NC_003888 SCBAC8D1.05, probable dihydropicolinate synthase, len: 298 aa; similar to SW:DAPA_RHIME (EMBL:AJ222715) Rhizobium meliloti dihydropicolinate synthase (EC 4.2.1.52) DapA, 300 aa; fasta scores: opt: 410 Z-score: 478.8 bits: 96.6 E(): 4.4e-19; 31.034% identity in 290 aa overlap. Contains Pfam match to entry PF00701 DHDPS, Dihydrodipicolinate synthetase family 1 4 6 2 [Reporter]SCO1857 (20A9)_PCR/6158 [Gene]SCO1857/5608 NC_003888 SCI39.04, probable bifunctional protein (CbiGH), len: 583 aa; N-terminal region similar to SW:CBIG_SALTY (EMBL:L12006) Salmonella typhimurium involved in cobalamin synthesis CbiG protein, 351 aa; fasta scores: opt: 312 Z-score: 304.6 bits: 65.6 E(): 2.2e-09; 32.986% identity in 288 aa overlap and C-terminal region similar to SW:CBIH_SALTY (EMBL:L12006) Salmonella typhimurium precorrin-3B C17-methyltransferase (EC 2.1.1.131) CbiH, 241 aa; fasta scores: opt: 684 Z-score: 658.7 bits: 130.6 E(): 4.3e-29; 46.862% identity in 239 aa overlap. Contains Pfam match to entry PF01890 CbiG, CbiG and PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases and match to Prosite entry PS00639 Eukaryotic thiol (cysteine) proteases histidine active site 1 4 5 22 [Reporter]SCO5365 (1A5)_PCR/6156 [Gene]SCO5365/5607 NC_003888 2SC6G5.09, possible transferase, len: 434 aa; similar to many putative transferases e.g. SW:Q10606 (RFE_MYCTU) putative undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase (EC 2.4.1.-) from Mycobacterium tuberculosis (404 aa); fasta scores; opt: 965, z-score: 1122.5, E(): 0, 43.7% identity in 375 aa overlap and SW:Q03521 (MRAY_BACSU) phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13) (324 aa) from Bacillus subtilis; fasta scores; opt: 252, z-score: 297.6, E(): 4.2e-09, 26.9% identity in 342 aa overlap. Contains Pfam match to entry PF00953 Glycos_transf_4, Glycosyl transferase. 1 4 5 21 [Reporter]SCO5368 (2M1)_PCR/6155 [Gene]SCO5368/5606 NC_003888 2SC6G5.12, atpE, ATP synthase C chain (EC 3.6.1.34), len: 76aa; strongly similar to many eg. SW:P12409 (ATPL_ANASP) ATP synthase C chain from Anabaena sp. (strain PCC 7120) (81 aa) fasta scores; opt: 194, z-score: 262.9, E(): 3.6e-07, 43.8% identity in 73 aa overlap. Identical to SW:P50014 (ATPL_STRLI) ATP synthase C chain from Streptomyces lividans. Contains Pfam match to entry PF00137 ATP-synt_C, ATP synthase subunit C, Prosite match to PS00605 ATP synthase c subunit signature and possible membrane-spanning hydrophobic regions. 1 4 5 20 [Reporter]SCO5879 (3I1)_PCR/6154 [Gene]SCO5879/5605 NC_003888 SC2E9.20, redW, acyl-coa dehydrogenase, len: 391; similar to many eg. ACDB_BACSU P45857 acyl-coa dehydrogenase (ec 1.3.99.-) (379 aa), fasta scores; opt: 831 z-score: 1005.0 E(): 0, 40.7% identity in 371 aa overlap. Contains Pfam match to entry Acyl-CoA_dh PF00441, Acyl-CoA dehydrogenases, score 308.86 1 4 5 19 [Reporter]SCO6158 (4E1)_PCR/6153 [Gene]SCO6158/5604 NC_003888 SC1A9.22c, unknown, len: 281 aa 1 4 5 18 [Reporter]SCO7092 (5A1)_PCR/6152 [Gene]SCO7092/5603 NC_003888 SC3A4.18, possible secreted protein, len: 300 aa. Contains a possible N-terminal signal sequence. Contains a TTA/leucine codon, possible target for bldA regulation 1 4 5 17 [Reporter]SCO7052 (5M21)_PCR/6151 [Gene]SCO7052/5602 NC_003888 SC4G1.18c, unknown, len: 131 aa 4 2 12 4 [Reporter]SCO1732 (18H19)_PCR/3773 [Gene]SCO1732/3429 NC_003888 SCI11.21, hypothetical protein, len: 44 aa; unknown function, possible CDS suggested by GC frameplot and the presence of a possible RBS 1 4 5 16 [Reporter]SCO7311 (6I21)_PCR/6150 [Gene]SCO7311/5601 NC_003888 SC5F8.21c, probable amino acid decarboxylase, len: 492 aa. Similar to many e.g. Bacillus subtilis SW:DCLY_BACSU (EMBL:X58433) lysine decarboxylase (EC 4.1.1.18) (490 aa), fasta scores: opt: 1108 z-score: 1197.8 E():0 35.6% identity in 494 aa overlap. Contains a Pfam match to entry PF01276 OKR_DC_1, Orn/Lys/Arg decarboxylase. 4 2 12 3 [Reporter]SCO1680 (19D19)_PCR/3772 [Gene]SCO1680/3428 NC_003888 SCI30A.01, partial CDS, probable gluconate permease, len: 359aa; similar to many eg. SW:GNTT_ECOLI high affinity gluconate transporter from Escherichia coli (437 aa) fasta scores; opt: 998, z-score: 1067.1, E(): 0, (42.1% identity in 359 aa overlap). Contains possible membrane spanning hydrophobic regions.,SCI52.22, probable gluconate permease (fragment), len: >144 aa; similar to N-terminal region of SW:GNUT_PSEAE (EMBL:AF026470) Pseudomonas aeruginosa gluconate permease GnuT or PA2322, 450 aa; fasta scores: opt: 370 Z-score: 442.1 bits: 89.4 E(): 4.8e-17; 44.262% identity in 122 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 5 15 [Reporter]SCO7421 (7E21)_PCR/6149 [Gene]SCO7421/5600 NC_003888 SC6D11.17c, cvnB10, unknown, len: 135 aa. Highly similar to many proteins of undefined function from Streptomyces coelicolor including: TR:CAB61281 (EMBL:AL132991) SCF55.11c (152 aa), fasta scores opt: 634 z-score: 769.3 E():0 71.9% identity in 128 aa overlap and TR:Q9X835(EMBL:AL049727) SC9B1.15C (139 aa), fasta scores opt: 329 z-score: 407.1 E(): 3e-15 40.9% identity in 132 aa overlap 4 2 12 2 [Reporter]SCO7704 (20L15)_PCR/3771 [Gene]SCO7704/3427 NC_003888 SCBAC12C8.05, possible TetR-family transcriptional regulator, len: 235 aa: similar to many from Streptomyces coelicolor e.g. TR:Q53901 (EMBL:M64683) ORF1-4 (ActII) (259 aa) fasta scores; opt: 310, Z-score: 362.1, 32.889% identity (35.071% ungapped) in 225 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and Pfam match to entry PF02909 tetR_C, Tetracyclin repressor, C-terminal all-alpha domain. 4 2 11 22 [Reporter]SCO1264 (1L11)_PCR/3769 [Gene]SCO1264/3426 NC_003888 2SCG18.11, probable hydrolase, len: 316 aa; similar to TR:CAB88827 (EMBL:AL353832) Streptomyces coelicolor putative hydrolase SCE6.23c, 294 aa; fasta scores: opt: 302 z-score: 345.4 E(): 9.3e-12; 31.5% identity in 276 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 4 2 11 21 [Reporter]SCO1494 (2H11)_PCR/3768 [Gene]SCO1494/3425 NC_003888 SC9C5.18c, aroB, 3-dehydroquinate synthase, len: 363 aa; similar to SW:AROB_ECOLI (EMBL:X03867) Escherichia coli 3-dehydroquinate synthase (EC 4.6.1.3) AroB, 362 aa; fasta scores: opt: 699 z-score: 796.4 E(): 0; 37.5% identity in 352 aa overlap. Contains Pfam match to entry PF01761 DHQ_synthase, 3-dehydroquinate synthase 4 2 11 20 [Reporter]SCO1482 (3D11)_PCR/3767 [Gene]SCO1482/3424 NC_003888 SC9C5.06c, pyrD, dihydroorotate dehydrogenase, len: 368 aa; similar to SW:PYRD_ECOLI (EMBL:X02826) Escherichia coli dihydroorotate dehydrogenase (EC 1.3.3.1) PyrD, 336 aa; fasta scores: opt: 874 z-score: 976.6 E(): 0; 43.2% identity in 336 aa overlap. Contains Pfam match to entry PF01180 DHOdehase, Dihydroorotate dehydrogenase and match to Prosite entry PS00912 Dihydroorotate dehydrogenase signature 2 4 2 11 19 [Reporter]SCO2251 (4P7)_PCR/3766 [Gene]SCO2251/3423 NC_003888 SC1G2.13, possible integral membrane protein, len: 224 aa. Contains multiple possible membrane spanning hydrophobic domains. 3 1 17 6 [Reporter]SCO5002 (17C4)_PCR/1365 [Gene]SCO5002/1249 NC_003888 SCK15.04c, conserved hypothetical protein, len: 216 aa; similar to TR:CAB77308 (EMBL:AL160312) Streptomyces coelicolor hypothetical 26.0 kD protein SCD35.24c, 241 aa; fasta scores: opt: 613 z-score: 732.4 E(): 0; 52.8% identity in 197 aa overlap 4 2 11 18 [Reporter]SCO2205 (5L7)_PCR/3765 [Gene]SCO2205/3422 NC_003888 SC3H12.13c, unknown, len: 104 aa 3 1 17 5 [Reporter]SCO1179 (17O24)_PCR/1364 [Gene]SCO1179/1248 NC_003888 SCG11A.10c, hypothetical protein, len: 252 aa; similar to various hypothetical proteins, e.g. TR:P95287 (EMBL:Z84498) Mycobacterium tuberculosis hypothetical 28.6 KD protein MTCY09F9.37c, 257 aa; fasta scores: opt: 729 z-score: 872.2 E(): 0; 48.3% identity in 238 aa overlap 4 2 11 17 [Reporter]SCO2055 (6H7)_PCR/3764 [Gene]SCO2055/3421 NC_003888 SC4G6.24, unknown, len: 525 aa; previously sequenced but annotated as two seperate hypothetical CDSs TR:Q53930 and TR:Q53931 (EMBL:X64420)) suggesting a sequence error which lead to a frameshift. Amino acid and codon usage plots support a single CDS. Also similar to TR:O88013 (EMBL:AL031107) hypothetical protein from Streptomyces coelicolor (527 aa) fasta scores; opt: 593, z-score: 699.2, E(): 1.3e-31, (36.0% identity in 545 aa overlap). Contains possible hydrophobic membrane spanning region at C-terminal domain 3 1 17 4 [Reporter]SCP1.235 (18K24)_PCR/1363 [Gene]SCP1.235/1247 NC_003888 SCP1.235, mmyP, hypothetical protein, len: 232aa; previously sequenced and annotated as TR:Q9JN84 (EMBL:AJ276673). Also similar to neighbouring CDS SCP1.245 mmfP (previously sequenced as TR:Q9JN92 (EMBL:AJ276673)) fasta scores; opt: 453, z-score: 556.3, E(): 1.6e-23, 38.9% identity in 234 aa overlap. 4 2 11 16 [Reporter]SCO7266 (7D7)_PCR/3763 [Gene]SCO7266/3420 NC_003888 SC5H1.26, probable 3-oxoacyl-[acyl-carrier protein] reductase, len: 245 aa; similar to many short-chain oxidoreductases e.g. SW:FABG_BACSU (EMBL:U59433), FabG, Bacillus subtilis 3-oxoacyl-[acyl-carrier protein] reductase (246 aa), fasta scores; opt: 521 z-score: 562.5 E(): 5.2e-24, 36.6% identity in 246 aa overlap and SW:DHG2_BACME (EMBL:D90044), GdhII, Bacillus megaterium glucose 1-dehydrogenase II (261 aa) (35.3% identity in 249 aa overlap). Similar to SCE134.03c (EMBL:AL049661) possible short chain dehydrogenase (255 aa) (35.1% identity in 242 aa overlap). Contains Pfam matches to entry PF00106 adh_short, short chain dehydrogenase, score 220.40, E-value 2.7e-62 and entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus, score 44.40, E-value 2.6e-09 3 1 17 3 [Reporter]SCO1379 (19G24)_PCR/1362 [Gene]SCO1379/1246 NC_003888 SC10A9.21, hypothetical protein, len: 131 aa; similar to TR:Q9RJP4 (EMBL:AL132991) Streptomyces coelicolor hypothetical 14.5 kDa protein SCF55.21c, 131 aa; fasta scores: opt: 195 z-score: 246.8 E(): 2.7e-06; 33.3% identity in 96 aa overlap 3 1 17 2 [Reporter]SCO0987 (20O20)_PCR/1361 [Gene]SCO0987/1245 NC_003888 SCBAC19F3.14, possible integral membrane protein, len: 517aa: similar to many eg. TR:Q9RCU8 (EMBL:AL136149) putative integral membrane protein from Streptomyces coelicolor (485 aa) fasta scores; opt: 581, Z-score: 572.1, 46.076% identity (48.211% ungapped) in 497 aa overlap. Contains possible membrane-spanning hydrophobic regions 3 1 16 22 [Reporter]SCO1326 (1O16)_PCR/1359 [Gene]SCO1326/1244 NC_003888 2SCG61.08, possible integral membrane lipid kinase, len: 291 aa; similar to many e.g. SW:P31054 (BACA_ECOLI) bacitracin resistance protein (putative undecaprenol kinase) (EC 2.7.1.66) from Escherichia coli (273 aa) fasta scores; opt: 283, z-score: 343.0, E(): 1.2e-11, 33.8% identity in 281 aa overlap and TR:O06239 (EMBL:Z95388) hypothetical protein from Mycobacterium tuberculosis (276 aa) fasta scores; opt: 439, z-score: 527.6, E(): 6.5e-22, 47.1% identity in 289 aa overlap. Contains possible membrane-spanning hydrophobic regions 3 1 16 21 [Reporter]SCO2229 (2K16)_PCR/1358 [Gene]SCO2229/1243 NC_003888 SC10B7.24c, malG, putative maltose permease, len: 303 aa; identical to previously sequenced TR:P72399 (EMBL:Y07706) Streptomyces coelicolor putative maltose permease MalG, 302 aa and similar to SW:MALG_ECOLI (EMBL:X02871) Escherichia coli maltose transport system permease protein MalG, 296 aa; fasta scores: opt: 476 z-score: 570.0 E(): 2.3e-24; 34.1% identity in 293 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 3 1 16 20 [Reporter]SCO7633 (3G16)_PCR/1357 [Gene]SCO7633/1242 NC_003888 SC10F4.06c, secreted endo-beta-N-acetylglucosaminidase, len: 309 aa; highly similar to SW:EBAG_STRPL (EMBL:K02182) Streptomyces plicatus endo-beta-n-acetylglucosaminidase H precursor (EC 3.2.1.96), 313 aa; fasta scores: opt: 1769 z-score: 1916.4 E(): 0; 86.9% identity in 312 aa overlap. Contains match to Prosite entry PS01095 Chitinases family 18 active site and possible N-terminal region signal peptide sequence 3 1 16 19 [Reporter]SCO2105 (4C16)_PCR/1356 [Gene]SCO2105/1241 NC_003888 SC2C1A.01c, possible transcriptional regulatory protein, partial CDS, len: > 57 aa. Similar to Mycobacterium tuberculosis TR:O53509 (EMBL:AL021957) hypothetical protein (146 aa), fasta scores opt: 137 z-score: 204.5 E(): 0.00048 42.3% identity in 71 aa overlap. Overlaps and extends into CDS SC4A10.38c on the adjoining cosmid. Contains a putative helix-turn-helix motif situated between residues 17..38 (+4.08 SD).,SC4A10.38c, conserved hypothetical protein, partial CDS, len: >86 aa; similar to mycobacterial hypothetical proteins TR:O53509 (EMBL:AL021957) Mycobacterium tuberculosis hypothetical protein (146 aa), fasta scores; opt: 198 z-score: 273.9 E(): 6.2e-08, 43.9% identity in 98 aa overlap and TR:O69567 (EMBL:AL022602) Mycobacterium leprae possible transcriptional regulator (134 aa) (44.8% identity in 96 aa overlap) 3 1 16 18 [Reporter]SCO6372 (5O12)_PCR/1355 [Gene]SCO6372/1240 NC_003888 SC4A2.08, questionable ORF, len: 77 aa 4 2 11 14 [Reporter]SCO7807 (9L3)_PCR/3761 [Gene]SCO7807/3419 NC_003888 SC8E7.04c, unknown, len: 92 aa. Contains 2 TTA leucine codons, possible targets for bldA regulation 4 2 11 13 [Reporter]SCO5121 (10H3)_PCR/3760 [Gene]SCO5121/3418 NC_003888 SC9E12.06, probable peptide transport system ATP-binding protein, len: 356 aa; similar to SW:APPF_BACSU (EMBL:U20909) Bacillus subtilis oligopeptide transport ATP-binding protein AppF, 329 aa; fasta scores: opt: 1255 z-score: 1332.6 E(): 0; 57.3% identity in 335 aa overlap, to TR:P72410 (EMBL:U68036) Streptomyces coelicolor putative ABC transporter intracellular ATPase subunit BldKE (fragment), 284 aa; fasta scores: opt: 999 z-score: 1063.6 E(): 0; 58.1% identity in 260 aa overlap and to TR:O86575 (EMBL:AL031184) Streptomyces coelicolor oligopeptide ABC transporter ATP-binding protein SC2A11.14, 496 aa; fasta scores: opt: 1306 z-score: 1117.3 E(): 0; 55.4% identity in 345 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 4 2 11 12 [Reporter]SCO2261 (11D3)_PCR/3759 [Gene]SCO2261/3417 NC_003888 SCC75A.07c, possible membrane protein, len: 85 aa. Contains a possible membrane spanning hydrophobic domains. 4 2 11 11 [Reporter]SCO4436 (11P23)_PCR/3758 [Gene]SCO4436/3416 NC_003888 SCD6.14, possible lyase, len: 136 aa; highly similar to TR:O86701 (EMBL:AL031515) Streptomyces coelicolor putative lyase SC5C7.04, 138 aa; fasta scores: opt: 532 z-score: 662.4 E(): 2e-29; 53.7% identity in 136 aa overlap 4 2 11 10 [Reporter]SCO2836 (12L23)_PCR/3757 [Gene]SCO2836/3415 NC_003888 SCE20.10, possible glycosyl transferase., len: 653 aa. Weakly similar to several other putative glycosyl transferases e.g. Aquifex aeolicus TR:O67406 (EMBL:AE000738) cellulose synthase catalytic subunit (759 aa), fasta scores opt: 326 z-score: 359.1 E(): 1.3e-12 22.0% identity in 590 aa overlap. Contains a Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases and multiple possible membrane spanning hydrophobic domains 4 2 11 9 [Reporter]SCO3643 (13H23)_PCR/3756 [Gene]SCO3643/3414 NC_003888 SCH10.21c, hypothetical protein, len: 329 aa; unknown function, weakly similar to SW:YT11_MYCTU (EMBL:Z79700) Mycobacterium tuberculosis hypothetical protein (266 aa), fasta scores; opt: 183 z-score: 199.4 E(): 0.00086, 30.7% identity in 225 aa overlap 4 2 11 8 [Reporter]SCO0902 (14D23)_PCR/3755 [Gene]SCO0902/3413 NC_003888 SCM1.35c, unknown, len: 142 aa. Similar to the C-terminus of several catechol 2,3-dioxygenases e.g. Sphingomonas sp. A8AN3 TR:Q9ZAY3(EMBL:U73127) catechol 2,3-dioxygenase (307 aa), fasta scores opt:128 z-score: 160.4 E():0.14 31.2% identity in 141 aa overlap. 3 1 16 17 [Reporter]SCO6540 (6K12)_PCR/1354 [Gene]SCO6540/1239 NC_003888 SC5C7.25, unknown, len: 101 aa; similar to several genes predicted to be pterin-4a-carbinolamine dehydratases e.g. Aquifex aeolicus TR:O66462 (EMBL:AE000671) phhB (99 aa), fasta scores; opt: 165 z-score: 280.7 E(): 2.2e-08, 30.3% identity in 89 aa overlap 4 2 11 7 [Reporter]SCO1038 (15P19)_PCR/3754 [Gene]SCO1038/3412 NC_003888 SCG20A.18, hypothetical protein, len: 115 aa; similar to TR:O06633 (EMBL:Z95618) Mycobacterium tuberculosis hypothetical 12.5 kD protein aa; fasta scores: opt: 138 z-score: 194.2 E(): 0.0024; 34.0% identity in 97 aa overlap 3 1 16 16 [Reporter]SCO3592 (7G12)_PCR/1353 [Gene]SCO3592/1238 NC_003888 SC66T3.03, possible membrane protein, len: 330 aa; similar to TR:Q9L038 (EMBL:AL352956) Streptomyces coelicolor putative membrane protein SC1H10.06c, 301 aa; fasta scores: opt: 557 Z-score: 630.1 E(): 1.8e-27; 50.968% identity in 310 aa overlap. Contains hydrophobic, possible membrane-spanning regions at the N-terminus region 4 2 11 6 [Reporter]SCO5170 (16L19)_PCR/3753 [Gene]SCO5170/3411 NC_003888 SCP8.33c, probable tetR-family transcriptional regulator, len: 213 aa; similar to TR:O53661 (EMBL:AL021929) Mycobacterium tuberculosis putative regulatory protein MTV034.04, 204 aa; fasta scores: opt: 309 z-score: 383.4 E(): 6.9e-14; 31.3% identity in 201 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 42..63 (+3.37 SD) 3 1 16 15 [Reporter]SCO5293 (8C12)_PCR/1352 [Gene]SCO5293/1237 NC_003888 SC6G9.40c, possible oxygenase subunit, len: 420 aa; similar to Pseudomonas spp. styrene monoxygenase large subunits e.g. TR:O06834 (EMBL:Z92524), styA, Pseudomonas fluorescens main component of styrene monooxygenase (415 aa), fasta scores; opt: 481 z-score: 542.1 E(): 7.3e-23, 31.2% identity in 417 aa overlap. It has no neighbouring CDS similar to the monoxygenase small subunit. The C-terminus overlaps the C-terminus of the downstream ORF on the opposite strand 4 2 11 5 [Reporter]SCO3153 (17H19)_PCR/3752 [Gene]SCO3153/3410 NC_003888 SCE87.04c, conserved hypothetical protein, len: 286 aa. Highly similar to many including: Mycobacterium tuberculosis SW:YA03_MYCTU (EMBL:Z94752) hypothetical 29.7 KD protein RV1003 (285 aa), fasta scores opt: 877 z-score: 992.7 E():0 53.6% identity in 278 aa overlap and Bacillus subtilis SW:YABC_BACSU (EMBL:D26185) hypothetical 33.0 KD protein (292 aa), fasta scores opt: 640 z-score: 726.6 E():0 41.3% identity in 276 aa overlap. Contains a Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases. 3 1 16 14 [Reporter]SCO6987 (9O8)_PCR/1351 [Gene]SCO6987/1236 NC_003888 SC8F11.13, putative membrane protein, len: 85 aa. Contains possible hydrophobic membrane spanning regions 3 1 16 13 [Reporter]SCO2516 (10K8)_PCR/1350 [Gene]SCO2516/1235 NC_003888 SCC121.19, possible secreted protein, len: 499 aa; similar to TR:O69831 (EMBL:AL023517) Streptomyces coelicolor putative secreted protease SC1B5.03C, 543 aa; fasta scores: opt: 772 z-score: 821.6 E(): 0; 32.2% identity in 537 aa overlap. Contains possible N-terminal region signal peptide sequence 3 1 16 12 [Reporter]SCO2549 (11G8)_PCR/1349 [Gene]SCO2549/1234 NC_003888 SCC77.16c, possible protease, len: 1067 aa. Similar to Thermoplasma acidophilum TR:P96086(EMBL:U72850) tricorn protease (1071 aa), fasta scores opt:opt: 937 z-score: 985.8 E():0 29.9% identity in 1091 aa overlap and Streptomyces coelicolor TR:CAB59664(EMBL:AL132674) putative protease SCE87.19 (1171 aa), fasta scores opt: 2252 z-score: 2374.0 E():0 48.1% identity in 1159 aa overlap. 3 1 16 11 [Reporter]SCO4466 (12C8)_PCR/1348 [Gene]SCO4466/1233 NC_003888 SCD65.09c, unknown, len: 162 aa 3 1 16 10 [Reporter]SCO3442 (13O4)_PCR/1347 [Gene]SCO3442/1232 NC_003888 SCE36.09, unknown, len: 114aa; 3 1 16 9 [Reporter]SCO0287 (14K4)_PCR/1346 [Gene]SCO0287/1231 NC_003888 SCF85.15, possible regulatory protein, len: 334 aa. similar to many e.g. Rhodococcus rhodochrous TR:P72312 (EMBL; D67026) nitrilase regulator NitR (319 aa), fasta scores opt: 538 z-score: 658.4 E(): 2.5e-29 30.6% identity in 307 aa overlap and tp SW:FEAR_ECOLI (EMBL:D67041) Escherichia coli transcriptional activator FeaR, 301 aa; fasta scores: opt: 290 Z-score: 343.6 E(): 1.7e-11; 23.970% identity in 267 aa overlap. Contains a Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family 3 1 16 8 [Reporter]SCO3903 (15G4)_PCR/1345 [Gene]SCO3903/1230 NC_003888 SCH24.25c, hypothetical protein, len: 343 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 4 2 11 4 [Reporter]SCO0182 (18D19)_PCR/3751 [Gene]SCO0182/3409 NC_003888 SCJ1.31c, doubtful CDS, len: 74 aa. Contains a TTA/leucine codon, possible target for bldA regulation. 4 2 11 3 [Reporter]SCO1309 (19P15)_PCR/3750 [Gene]SCO1309/3408 NC_003888 SCBAC36F5.20c, possible regulatory protein, len: 115 aa; similar to TR:O53838 (EMBL:AL022004) Mycobacterium tuberculosis putative transcriptional regulator RV0827c or MTV043.19c, 130 aa; fasta scores: opt: 243 Z-score: 311.9 bits: 63.2 E(): 9.2e-10; 39.252% identity in 107 aa overlap 4 2 11 2 [Reporter]SCO2821 (20H15)_PCR/3749 [Gene]SCO2821/3407 NC_003888 SCBAC17F8.12c, possible secreted pectate lyase, len: 521 aa: similar to many e.g. TR:Q9KGY6 (EMBL:AF278705) pectate lyase B from Alteromonas haloplanktis (658 aa) fasta scores; opt: 1744, Z-score: 1590.1, 63.301% identity (65.859% ungapped) in 515 aa overlap and SW:P04959 (PELB_ERWCH) pectate lyase B from Erwinia chrysanthemi (375 aa) fasta scores; opt: 413, Z-score: 386.2, 29.167% identity (32.464% ungapped) in 384 aa overlap. Contains Pfam match to entry PF00544 pec_lyase, Pectate lyase and a probable N-terminal signal sequence. 4 2 10 22 [Reporter]SCO2802 (1H11)_PCR/3747 [Gene]SCO2802/3406 NC_003888 2SCC13.10, possible secreted protein, len: 193 aa. Contains possible N-terminal region signal peptide sequence 4 2 10 21 [Reporter]SCO1579 (2D11)_PCR/3746 [Gene]SCO1579/3405 NC_003888 SCL24.15c, argJ, glutamate N-acetyltransferase (fragment), len: >228 aa; highly similar to TR:P94988 (EMBL:Z85982) Mycobacterium tuberculosis ArgJ, 404 aa; fasta scores: opt: 782 z-score: 919.7 E(): 0; 57.1% identity in 226 aa overlap and to SW:ARGJ_CORGL (EMBL:X86157) Corynebacterium glutamicum glutamate N-acetyltransferase (EC 2.3.1.35) ArgJ, 388 aa; fasta scores: opt: 730 z-score: 859.3 E(): 0; 50.4% identity in 230 aa overlap. Contains Pfam match to entry PF01960 ArgJ, ArgJ family,SCI35.01c, argJ, probable glutamate N-acetyltransferase, partial CDS, len: >184 aa; similar to many e.g. ARGJ_CORGL glutamate N-acetyltransferase (ornithine acetyltransferase) (EC 2.3.1.35) (388 aa), fasta scores; opt: 614 z-score: 909.0 E(): 0, 55.4% identity in 184 aa overlap. Referred to as 'argB' on genetic map 4 2 10 20 [Reporter]SCO4706 (3P7)_PCR/3745 [Gene]SCO4706/3404 NC_003888 SCD31.31, rpsS, 30S ribosomal protein S19, len: 93 aa; highly similar to SW:RS19_BACST (EMBL:X54994) Bacillus stearothermophilus 30S ribosomal protein S19 RpsS, 91 aa; fasta scores: opt: 448 z-score: 642.9 E(): 2.2e-28; 67.8% identity in 90 aa overlap. Contains Pfam match to entry PF00203 Ribosomal_S19, Ribosomal protein S19 and match to Prosite entry PS00323 Ribosomal protein S19 signature 4 2 10 19 [Reporter]SCO2247 (4L7)_PCR/3744 [Gene]SCO2247/3403 NC_003888 SC1G2.09c, unknown, len: 61 aa. 3 1 16 7 [Reporter]SCO2939 (16C4)_PCR/1344 [Gene]SCO2939/1229 NC_003888 SCE56.03c, unknown, len: 260 aa 4 2 10 18 [Reporter]SCO5735 (5H7)_PCR/3743 [Gene]SCO5735/3402 NC_003888 SC3C3.21, probable integral membrane protein, len: 491 aa; similar to hypothetical membrane protein from M. tuberculosis TR:O06265 (EMBL:Z95389) MTCY77.20 Rv3448 (467 aa), fasta scores; opt: 192 z-score: 322.0 E(): 1.1e-10, 26.9% identity in 495 aa overlap 3 1 16 6 [Reporter]SCO1195 (16O24)_PCR/1343 [Gene]SCO1195/1228 NC_003888 SCG11A.26c, putative membrane protein, len: 64 aa. Contains possible hydrophobic membrane spanning regions 4 2 10 17 [Reporter]SCO2051 (6D7)_PCR/3742 [Gene]SCO2051/3401 NC_003888 SC4G6.20c, hisH, amidotransferase, len: 222aa; previously sequenced therefoer identical to SW:HIS5_STRCO. Contains Pfam match to entry PF00117 GATase, Glutamine amidotransferase class-I and Prosite match to PS00442 Glutamine amidotransferases class-I active site. 3 1 16 5 [Reporter]SCO0242 (17K24)_PCR/1342 [Gene]SCO0242/1227 NC_003888 SCJ9A.21, unknown, len: 147aa; similar to TR:Q98JA0 (EMBL:AP002998) MLR2034 protein from Rhizobium loti (Mesorhizobium loti) (145 aa) fasta scores; opt: 334, Z-score: 400.1, E(): 1.2e-14, 37.324% identity (37.324% ungapped) in 142 aa overlap. Weak similarity to SW:YAER_ECOLI hypothetical protein from Escherichia coli (129 aa) fasta scores; opt: 105, z-score: 139.5, E(): 2.1, (25.0% identity in 148 aa overlap). 4 2 10 16 [Reporter]SCO3617 (7P3)_PCR/3741 [Gene]SCO3617/3400 NC_003888 SC66T3.28c, hypothetical protein, len: 219 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 3 1 16 4 [Reporter]SCO0818 (18G24)_PCR/1341 [Gene]SCO0818/1226 NC_003888 SCF43A.08, probable ABC transporter ATP-binding protein, len: 264 aa; similar to many hypothetical ABC transporter ATP-binding proteins e.g. TR:O28803 (EMBL:AE001001) Archaeoglobus fulgidus putative ABC transporter ATP-binding protein (226 aa), fasta scores; opt: 675 z-score: 764.2 E(): 0, 48.2% identity in 220 aa overlap. Alternative start codons are present at codons 2 and 4. Similar to many from S.coelicolor e.g. TR:O54136 (EMBL:AL021530) S.coelicolor probable ABC-transporter ATP binding protein (230 aa) (44.5% identity in 218 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 16 3 [Reporter]SCP1.233A (19C24)_PCR/1340 [Gene]SCP1.233A/1225 NC_003888 SCP1.233A, mmyX, possible ATP/GTP-binding protein, len: 234aa; previously sequenced and annotated as TR:Q9JN81 (EMBL:AJ276673). Also similar to neighbouring CDS SCP1.236 MmyK, putative ATP/GTP-binding protein (206 aa)(previously sequenced as TR:Q9JN85 (EMBL:AJ276673) hypothetical protein) fasta scores; opt: 538, z-score: 647.7, E(): 1.3e-28, 42.7% identity in 199 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 1 16 2 [Reporter]SCO5105 (20K20)_PCR/1339 [Gene]SCO5105/1224 NC_003888 SCBAC31E11.01, possible membrane protein, len: 410 aa. Contains possible hydrophobic membrane spanning region 3 1 15 22 [Reporter]SCO1322 (1K16)_PCR/1337 [Gene]SCO1322/1223 NC_003888 2SCG61.04c, unknown, len: 301aa; contains a serine-rich region. 3 1 15 21 [Reporter]SCO2789 (2G16)_PCR/1336 [Gene]SCO2789/1222 NC_003888 SCC105.20, glmS2, glucosamine-fructose-6-phosphate aminotransferase, len: 605 aa; similar to SW:GLMS_ECOLI (EMBL:X01631) Escherichia coli glucosamine-fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) GlmS, 608 aa; fasta scores: opt: 1617 z-score: 1815.6 E(): 0; 44.1% identity in 612 aa overlap and highly similar to TR:BAA86263 (EMBL:AB023785) Streptomyces griseus ORF3 (fragment), 589 aa; fasta scores: opt: 3364 z-score: 3776.2 E(): 0; 87.6% identity in 589 aa overlap and also to SW:GLMS_STRCO (EMBL:AL031317) Streptomyces coelicolor GlmS1, probable glucosamine--fructose-6-phosphate aminotransferase SC6G4.18, 615 aa; fasta scores: opt: 1480 z-score: 1526.7 E(): 0; 43.8% identity in 621 aa overlap. Contains Pfam matches to entries PF00310 GATase_2, Glutamine amidotransferases class-II and 2x PF01380 SIS, SIS domain 3 1 15 20 [Reporter]SCO7804 (3C16)_PCR/1335 [Gene]SCO7804/1221 NC_003888 SC8E7.01, possible membrane protein, partial CDS, len: >59 aa. Contains possible membrane spanning hydrophobic domains.,SC10B8A.14, unknown, len: 59aa; 3 1 15 19 [Reporter]SCO7033 (4O12)_PCR/1334 [Gene]SCO7033/1220 NC_003888 SC1H10.22c, possible regulatory protein, len: 555 aa. Weakly similar to Streptomyces ambofaciens TR:Q00509(EMBL:X63451) polyketide synthase regulatory gene, SrmR (604 aa), fasta scores opt: 252 z-score: 283.4 E(): 2.3e-08 27.0% identity in 470 aa overlap. Contains putative helix-turn-helix motif situated between residues 494..515 (+5.41 SD). 3 1 15 18 [Reporter]SCO6368 (5K12)_PCR/1333 [Gene]SCO6368/1219 NC_003888 SC4A2.04c, probable lipoprotein, len: 206 aa; similar to TR:Q98E07 (EMBL:AP003004) Rhizobium loti (Mesorhizobium loti) putative lipoprotein MLR4460, 201 aa; fasta scores: opt: 472 Z-score: 495.8 E(): 5.6e-20; 40.933% identity in 193 aa overlap. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 3 1 15 17 [Reporter]SCO5811 (6G12)_PCR/1332 [Gene]SCO5811/1218 NC_003888 SC5B8.01, probable transcriptional regulator, partial CDS, overlaps and extends SC4H2.32, len: > 140 aa,SC4H2.32, probable transcriptional regulator, partial CDS, len: >111 aa; similar to many e.g. TR:D1022772 (EMBL:D85417) HemR from Propionibacterium freudenreichii (243 aa), fasta scores; opt: 262 z-score: 366.7 E(): 3.3e-13, 46.5% identity in 101 aa overlap. Contains Pfam match to entry tetR PF00440, Bacterial regulatory proteins, tetR family, score 29.06 3 1 15 16 [Reporter]SCO5705 (7C12)_PCR/1331 [Gene]SCO5705/1217 NC_003888 SC5H4.29, hypothetical protein, len: 101 aa; similar to SW:YLXR_BACSU (EMBL:Z18631) Bacillus subtilis hypothetical 10.4 kD protein in NusA-InfB intergenic region (ORF3) YlxR, 91 aa; fasta scores: opt: 130 z-score: 176.4 E(): 0.023; 29.7% identity in 91 aa overlap. Contains and match to Prosite entry 3 1 15 15 [Reporter]SCO2486 (8O8)_PCR/1330 [Gene]SCO2486/1216 NC_003888 SC7A8.25, nirB, probable nitrite reductase, len: 410 aa; similar to N-terminal region of TR:O53674 (EMBL:AL021929) Mycobacterium tuberculosis nitrite reductase large subunit NirB, 853 aa; fasta scores: opt: 657 z-score: 711.6 E(): 3e-32; 37.8% identity in 431 aa overlap and to SW:NIRB_KLEPN (EMBL:L06800) Klebsiella pneumoniae nitrite reductase [NAD(P)H] large subunit (EC 1.6.6.4) NasB, 957 aa; fasta scores: opt: 636 z-score: 688.3 E(): 6e-3; 35.4% identity in 412 aa overlap. Contains possible N-terminal region signal peptide sequence 3 1 15 14 [Reporter]SCO6983 (9K8)_PCR/1329 [Gene]SCO6983/1215 NC_003888 SC8F11.09, unknown, len: 137 aa. Weakly similar to a protein of undefined function from Mycobacterium tuberculosis SW:Y887_MYCTU(EMBL:Z73101) (152 aa), fasta scores opt: 193 z-score: 239.5 E(): 6.6e-06 31.7% identity in 139 aa overlap. 3 1 15 12 [Reporter]SCO2545 (11C8)_PCR/1327 [Gene]SCO2545/1214 NC_003888 SCC77.12, possible transmembrane efflux protein, len: 449 aa. Weakly similar to Streptomyces lividans SW:CMLR_STRLI(EMBL:X59968) chloramphenicol resistance protein CmlR (392 aa), fasta scores opt: 234 z-score: 234.3 E(): 1.2e-05 27.5% identity in 396 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 3 1 15 11 [Reporter]SCO3941 (12O4)_PCR/1326 [Gene]SCO3941/1213 NC_003888 SCD78.08c, probable serine/threonine protein kinase, len: 498 aa, identical to TR:G3694948 (EMBL:AF090690) previously sequenced putative serine/threonine S. coelicolor. Also similar to TR:O69213 (EMBL:AJ224354) serine-threonine phosphatase PrpA from Anabaena sp. strain PCC 7120 (858 aa) fasta scores; opt:102, z-score: 234.4, E(): 9e-06, (26.8% identity in 231 aa overlap) contains PS00070 aldehyde dehydrogenases cysteine active site. 3 1 15 10 [Reporter]SCO3438 (13K4)_PCR/1325 [Gene]SCO3438/1212 NC_003888 SCE36.05c, unknown, len: 407aa; contains a repeat region. 3 1 15 9 [Reporter]SCO0291 (14G4)_PCR/1324 [Gene]SCO0291/1211 NC_003888 SCF85.19, possible binding protein dependent transport protein, len: 300 aa. Similar to many including: Streptomyces lividans TR:Q9ZI17 (EMBL; AF043654) BxlF (317 aa), fasta scores opt: 873 z-score: 1049.2 E(): 0 44.0% identity in 298 aa overlap and Streptomyces coelicolor TR:CAB41203 (EMBL; AL049661)putative binding protein dependent transport protein SCE134.05C (327 aa), fasta scores opt: 551 z-score: 664.3 E(): 1.2e-29 32.7% identity in 294 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and a PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains multiple membrane-spanning hydrophobic domains. 3 1 15 8 [Reporter]SCO0357 (15C4)_PCR/1323 [Gene]SCO0357/1210 NC_003888 SCF41.16c, probable GntR-family regulator (putative secreted protein), len: 270 aa. Similar to Streptomyces coelicolor TR:CAB50881 (EMBL; AL096844) putative GntR-family transcriptional regulator SCI28.07 (230 aa), fasta scores opt: 292 z-score: 343.8 E(): 8.5e-12 32.7% identity in 220 aa overlap and Escherichia coli SW:PDHR_ECOLI (EMBL; V01498) pyruvate dehydrogenase complex repressor(254 aa), fasta scores opt: 253 z-score: 298.6 E(): 2.8e-09 30.4% identity in 224 aa overlap. Contains a Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. 3 1 15 7 [Reporter]SCO0178 (15O24)_PCR/1322 [Gene]SCO0178/1209 NC_003888 SCJ1.27, unknown, len: 148 aa. Weak similarity to Mycobacterium tuberculosis SW:YW23_MYCTU (EMBL; Z74025) hypothetical 33.9 KD protein MTCY39.23C (317 aa), fasta scores opt: 201 z-score: 231.7 E(): 1.5e-05 33.1% identity in 136 aa overlap and Mycobacterium tuberculosis TR:O06189 (EMBL; Z95387)hypothetical 31.7 KD protein MTCY1A10.09C (297 aa), fasta scores opt: 199 z-score: 229.8 E(): 1.9e-05 33.3% identity in 153 aa overlap. 3 1 15 6 [Reporter]SCO0604 (16K24)_PCR/1321 [Gene]SCO0604/1208 NC_003888 SCF55.28c, unknown, len: 391 aa; similar to TR:AAK83171 (EMBL:AF333038) AVIX9 from Streptomyces viridochromogenes. 3 1 15 5 [Reporter]SCO3772 (17G24)_PCR/1320 [Gene]SCO3772/1207 NC_003888 SCH63.19c, possible secreted protein, len: 348 aa; similar to TR:AAG03932 (EMBL:AE004490) Pseudomonas aeruginosa hypothetical 35.1 kDa protein PA0543, 322 aa; fasta scores: opt: 419 z-score: 410.9 E(): 2.3e-15; 33.6% identity in 295 aa overlap. Contains possible N-terminal region signal peptide sequence 3 1 15 4 [Reporter]SCO0546 (18C24)_PCR/1319 [Gene]SCO0546/1206 NC_003888 SCF11.26c, pyc, pyruvate carboxylase, len: 1124 aa; highly similar to TR:Q9F843 (EMBL:AF262949) Mycobacterium smegmatis pyruvate carboxylase (EC 6.4.1.1) Pyc, 1127 aa; fasta scores: opt: 5701 Z-score: 5851.2 E(): 0; 76.356% identity in 1125 aa overlap and similar to TR:O54587 (EMBL:AF038548) Corynebacterium glutamicum pyruvate carboxylase Pyc, 1140 aa; fasta scores: opt: 4925 z-score: 5186.1 E(): 0; 65.2% identity in 1128 aa overlap. Contains Pfam taches to entries PF00364 biotin_lipoyl, biotin-requiring enzymes, PF00682 HMGL-like, HMGL-like and PF00289 CPSase_L_chain, carbamoyl-phosphate synthase (CPSase) and prosite match to entry PS00867 carbamoyl-phosphate synthase subdomain signature 2 3 1 15 3 [Reporter]SCO4878 (19O20)_PCR/1318 [Gene]SCO4878/1205 NC_003888 2SCK8.04c, possible glycosyltransferase, len: 326 aa; similar to TR:AAG44714 (EMBL:AF267127) Lactobacillus delbrueckii glycosyltransferase EpsJ, 327 aa; fasta scores: opt: 319 z-score: 393.4 E(): 2.5e-14; 30.5% identity in 236 aa overlap. Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases 3 1 15 2 [Reporter]SCO5112 (20G20)_PCR/1317 [Gene]SCO5112/1204 NC_003888 SCBAC31E11.08, bldKA, possible ABC transport system integral membrane protein, len: 343 aa; identical to previously sequenced TR:P72406 (EMBL:U68036) Streptomyces coelicolor BldKA, 335 aa. Methionine start codon 9 amino acids upstream from the previous sequence start; possible frameshift from frame 2 into frame 1 in the previously sequenced DNA. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 3 1 14 22 [Reporter]SCO1014 (1G16)_PCR/1315 [Gene]SCO1014/1203 NC_003888 2SCG2.28c, probable transferase, len: 537 aa; similar to SW:LNT_ECOLI (EMBL:X58070) Escherichia coli apolipoprotein N-acyltransferase (EC 2.3.1.-) CutE, 512 aa; fasta scores: opt: 328 z-score: 352.1 E(): 4.4e-12; 26.1% identity in 533 aa overlap. Contains Pfam match to entry PF00795 CN_hydrolase, Carbon-nitrogen hydrolase. Also contains possible hydrophobic membrane spanning regions 3 1 14 21 [Reporter]SCO5444 (2C16)_PCR/1314 [Gene]SCO5444/1202 NC_003888 SC3D11.01, glgP, possible glycogen phosphorylase, len: >201aa; previously sequenced as TR:O70011 (EMBL:AJ001205). Also similar to TR:Q9YGA7 (EMBL:AF115479) maltodextrin phosphorylase from Thermococcus litoralis (831 aa) fasta scores; opt: 242, z-score: 283.2, E(): 2.3e-08, 30.9% identity in 194 aa overlap.,SC6A11.20, glgP, possible glycogen phosphorylase (fragment), len: >705 aa; previously sequenced as TR:O70011 (EMBL:AJ001205). Also similar to TR:Q9YGA7 (EMBL:AF115479) maltodextrin phosphorylase from Thermococcus litoralis (831 aa) fasta scores; opt: 849, z-score: 972.3, E(): 0, 40.5% identity in 699 aa overlap. Contains Prosite match to PS00102 Phosphorylase pyridoxal-phosphate attachment site 3 1 14 20 [Reporter]SCO5929 (3O12)_PCR/1313 [Gene]SCO5929/1201 NC_003888 SC10A5.34c, possible oxidoreductase, len: 258 aa; very weakly similar to many eg. TR:G2673979 (EMBL:U94980) 2,4 -DIENOYL-COA REDUCTASE (HUMAN) (335 aa), fasta scores; opt: 167 z-score: 231.2 E(): 1.1e-05, 21.2% identity in 208 aa overlap. Contains PS01070 DNA/RNA non-specific endonucleases active site 3 1 14 19 [Reporter]SCO7029 (4K12)_PCR/1312 [Gene]SCO7029/1200 NC_003888 SC1H10.18, possible binding-protein-dependent transport protein, len: 318 aa. Almost identical to Streptomyces lividans TR:Q9ZI17 (EMBL:AF043654) putative permease, BxlF (317 aa), fasta scores opt:2000 z-score: 2320.3 E():0 98.1% identity in 318 aa overlap. Also similar to Streptococcus mutans SW:MSMF_STRMU(EMBL:M77351) multiple sugar-binding transport system permease protein, MsmF (290 aa), fasta scores opt: 447 z-score: 524.7 E(): 8.3e-22 29.9% identity in 271 aa overlap and Streptomyces coelicolor TR:CAB54176(EMBL:AL110470) putative binding-protein-dependent transport protein, SCF85.19 (300 aa), fasta scores opt: 898 z-score: 1046.1 E():0 44.6% identity in 298 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and multiple possible membrane spanning hydrophobic domains. 3 2 13 7 [Reporter]SCO1037 (15G23)_PCR/3294 [Gene]SCO1037/2999 NC_003888 SCG20A.17, hypothetical protein, len: 133 aa; similar to TR:O32183 (EMBL:Z99120) Bacillus subtilis hypothetical protein YusQ, 127 aa; fasta scores: opt: 280 z-score: 359.2 E(): 1.6e-12; 36.4% identity in 129 aa overlap 3 2 13 6 [Reporter]SCO3128 (16C23)_PCR/3293 [Gene]SCO3128/2998 NC_003888 SCE66.07, possible fatty acid desaturase (membrane), len: 336 aa; similar to many (mainly eukaryotic) e.g. SW:O00767 (ACOD_HUMAN) acyl-CoA desaturase (EC 1.14.99.5) from Homo sapiens (Human) (359 aa) fasta scores; opt: 409, z-score: 480.0, E(): 2.9e-19, 29.7% identity in 330 aa overlap. Contains Pfam match to entry PF01069 Desaturase, Fatty acid desaturase and possible membrane-spanning hydrophobic regions. 3 2 13 5 [Reporter]SCO0505 (17O19)_PCR/3292 [Gene]SCO0505/2997 NC_003888 SCF6.01, possible multi-domain protein, (fragment), len: >152 aa. Highly similar to Amycolatopsis orientalis TR:CAB45032 (EMBL; AL078635) putative large multi-functional protein (987 aa), fasta scores opt: 595 z-score: 714.7 E(): 1.9e-32 58.6% identity in 152 aa overlap.,SCF34.24, possible large, multifunctional secreted protein, partial CDS, len: >891 aa; contains a region of weak similarity to members of the glycosyl hydrolase family at approx. aa 700-890 e.g. to part of SW:CTA2_BACCI (EMBL:D88360), Cit, Bacillus circulans cycloisomaltooligosaccharide glucanotransferase precursor (964 aa), fasta scores; opt: 279 z-score: 292.6 E(): 6.1e-09, 33.3% identity in 213 aa overlap and region of very weak similarity at approx. aa 200-400 to part of a dehydrogenase SW:SNDH_ACELI (EMBL:D28511) Acetobacter liquefaciens L-sorbosone dehydrogenase (449 aa) (26.2% identity in 279 aa overlap). Similar to CZA382.11 (EMBL:AL078635) Amycolatopsis orientalis possible large, multifunctional protein (987 aa) (63.2% identity in 880 aa overlap). Contains a probable N-terminal signal sequence 3 2 13 4 [Reporter]SCO1315 (18K19)_PCR/3291 [Gene]SCO1315/2996 NC_003888 SCBAC36F5.26, possible siderophore binding protein, len: 178 aa; similar to TR:Q9V158 (EMBL:AJ248284) Pyrococcus abyssi ferripyochelin binding protein Fbp or PAB0393, 173 aa; fasta scores: opt: 525 Z-score: 583.9 bits: 114.5 E(): 6.5e-25; 49.068% identity in 161 aa overlap. Contains 4x Pfam matches to entry PF00132 hexapep, Bacterial transferase hexapeptide (four repeats) 3 2 13 3 [Reporter]SCO3998 (19G19)_PCR/3290 [Gene]SCO3998/2995 NC_003888 2SC10A7.02c, unknown, len: 447 aa. Contains 3x Pfam matches to entry PF01011 Bacterial_PQQ, PQQ enzyme repeat 3 2 13 2 [Reporter]SCO7496 (20O15)_PCR/3289 [Gene]SCO7496/2994 NC_003888 SCBAC17A6.29c, conserved hypothetical integral membrane protein, len: aa; similar to many e.g. TR:Q9I5E8 (EMBL:AE004514) hypothetical protein from Pseudomonas aeruginosa (271 aa) fasta scores: opt: 560, Z-score: 552.2, 44.944% identity (48.387% ungapped) in 267 aa overlap. Contains multiple hydrophobic possible membrane spanning regions 3 2 12 22 [Reporter]SCO1267 (1O11)_PCR/3287 [Gene]SCO1267/2993 NC_003888 2SCG18.14c, probable acyl carrier protein, len: 85 aa; similar to TR:O54150 (EMBL:AL021409) Streptomyces coelicolor acyl carrier protein SC3F7.07c, 81 aa; fasta scores: opt: 175 z-score: 253.1 E(): 1.3e-06; 38.7% identity in 75 aa overlap and to SW:ACP_RHIME (EMBL:AF159244) Rhizobium meliloti acyl carrier protein AcpP, 77 aa; fasta scores: opt: 118 z-score: 177.8 E(): 0.02; 33.3% identity in 66 aa overlap. Contains Pfam match to entry PF00550 pp-binding, Phosphopantetheine attachment site 3 2 12 21 [Reporter]SCO3096 (2K11)_PCR/3286 [Gene]SCO3096/2992 NC_003888 SCE41.05c, eno, enolase, len: 426 aa; similar to SW:ENO_ECOLI (EMBL:X82400) Escherichia coli enolase (EC 4.2.1.11) Eno, 431 aa; fasta scores: opt: 1603 z-score: 1807.6 E(): 0; 60.6% identity in 419 aa overlap. Contains Pfam match to entry PF00113 enolase, Enol-ase and match to Prosite entry PS00164 Enolase signature 3 2 12 20 [Reporter]SCO1488 (3G11)_PCR/3285 [Gene]SCO1488/2991 NC_003888 SC9C5.12c, pyrR, pyrimidine operon regulatory protein, len: 193 aa; similar to SW:PYRR_BACCL (EMBL:X76083) Bacillus caldolyticus pyrimidine operon regulatory protein PyrR, 179 aa; fasta scores: opt: 634 z-score: 747.8 E(): 0; 58.5% identity in 176 aa overlap. Contains Pfam match to entry PF00156 Pribosyltran, Phosphoribosyl transferase domain 3 2 12 19 [Reporter]SCO6015 (4C11)_PCR/3284 [Gene]SCO6015/2990 NC_003888 SC1C3.03c, unknown, len: 125 aa; some similarity to a hypothetical protein from M. tuberculosis TR:E1256517 (EMBL:AL022073) MTV051.14 (135 aa), fasta scores; opt: 223 z-score: 329.2 E(): 4.2e-11, 39.8% identity in 113 aa overlap 3 2 12 18 [Reporter]SCO2071 (5O7)_PCR/3283 [Gene]SCO2071/2989 NC_003888 SC4A10.04c, possible Na+/H+ antiporter, len: 528 aa; shows weak similarity to the N-terminal portion of eukaryotic Na+/H+ antiporters e.g. SW:NAH3_RAT (EMBL:M85300) Rattus norvegicus sodium/hydrogen exchanger 3 (831 aa), fasta scores; opt: 271 z-score: 293.1 E(): 5.3e-09, 25.8% identity in 481 aa overlap. Similar to many putative Na+/H+ antiporters e.g. SW:YU23_MYCTU (EMBL:Z77163) Mycobacterium tuberculosis putative Na(+)/H(+) exchanger (542 aa) (31.4% identity in 528 aa overlap). Shows weak similarity to SC66T3.14c (EMBL:AL079348) S.coelicolor probable Na(+)/H(+) antiporter (514 aa) (27.5% identity in 545 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF00999 Na_H_Exchanger, Sodium/hydrogen exchanger family 3 2 12 17 [Reporter]SCO5830 (6K7)_PCR/3282 [Gene]SCO5830/2988 NC_003888 SC5B8.20c, unknown, len: 313 aa; very weak similarit y to a hypothetical protein from Synechocystis TR:P72597 (E MBL:D90899) (321 aa), fasta scores; opt: 232 z-score: 291.9 E(): 4.6e-09, 23.1% identity in 268 aa overlap 3 2 12 16 [Reporter]SCO7263 (7G7)_PCR/3281 [Gene]SCO7263/2987 NC_003888 SC5H1.29c, chiF, chitinase, len: 296 aa; previously sequenced as chiF (EMBL:AB017012) and similar to many e.g. TR:O50152 (EMBL:AB009289), chiC, Streptomyces griseus chitinase C (294 aa), fasta scores; opt: 1308 z-score: 1506.5 E(): 0, 65.8% identity in 295 aa overlap. Contains possible N-terminal signal sequence. Contains Pfam match to entry PF00182 chitinase_1, Chitinases class I, score 247.20, E-value 2.2e-70 3 2 12 15 [Reporter]SCO4760 (8C7)_PCR/3280 [Gene]SCO4760/2986 NC_003888 SC6G4.38, probable secreted protein, len: 249 aa; slight similarity to S. lividans TR:Q54413 (EMBL:M64552) acetyl-xylan esterase precursor (335 aa), fasta scores; opt: 16 3 z-score: 197.4 E(): 0.00099, 28.9% identity in 180 aa overlap. Contains N-terminal signal sequence 3 2 12 14 [Reporter]SCO7810 (9O3)_PCR/3279 [Gene]SCO7810/2985 NC_003888 SC8E7.07, possible oxidoreductase, len: 254 aa. Highly similar to many putative reductases e.g. Streptomyces coelicolor TR:O86853(EMBL:AJ007731) putative 3-ketoacyl-ACP/CoA reductase (244 aa), fasta scores opt:777 z-score: 840.5 E():0 51.2% identity in 244 aa overlap. Contains a Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus. 3 2 12 13 [Reporter]SCO6267 (10K3)_PCR/3278 [Gene]SCO6267/2984 NC_003888 SCAH10.32, hypothetical protein orfA, len: 284 aa; identical to previously sequenced TR:O86850 (EMBL:AJ007731) Streptomyces coelicolor hypothetical 31.0 kD protein, 284 aa and to various hypothetical proteins, e.g. TR:O68920 (EMBL:AF058302) Streptomyces roseofulvus FrnS, 305 aa; fasta scores: opt: 591 z-score: 680.7 E(): 1.5e-30; 41.5% identity in 282 aa overlap 3 2 12 12 [Reporter]SCO2264 (11G3)_PCR/3277 [Gene]SCO2264/2983 NC_003888 SCC75A.10, possible integral membrane protein, len: 431 aa. Weakly similar to Mycobacterium smegmatis TR:O31137(EMBL:AF030344) tetracycline-resistance determinant (419 aa), fasta scores opt: 330 z-score: 364.8 E(): 6e-13 24.2% identity in 414 aa overlap. Highly similar to Streptomyces coelicolor TR:O69821(EMBL:AL023496) transmembrane protein SC1A6.16 (431 aa), fasta scores opt: 1808 z-score: 1971.5 E():0 64.7% identity in 419 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 3 2 12 11 [Reporter]SCO4775 (12C3)_PCR/3276 [Gene]SCO4775/2982 NC_003888 SCD63.07, pkaH, serine/threonine protein kinase, len: 717 aa; similar to TR:Q9XAI1 (EMBL:AL079348) Streptomyces coelicolor putative serine/threonine protein kinase SC66T3.32c, 783 aa; fasta scores: opt: 570 z-score: 386.8 E(): 4.1e-14; 30.6% identity in 687 aa overlap and to SW:PKAA_STRCO (EMBL:D86821) Streptomyces coelicolor serine/threonine protein kinase PkaA (EC 2.7.1.-), 543 aa; fasta scores: opt: 543 z-score: 370.9 E(): 3.1e-13; 29.8% identity in 476 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and matches to Prosite entries PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature and possible hydrophobic membrane spanning region 3 2 12 10 [Reporter]SCO2839 (12O23)_PCR/3275 [Gene]SCO2839/2981 NC_003888 SCE20.13c, possible lipoprotein, len: 190 aa. Contains an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. 3 2 12 9 [Reporter]SCO0119 (13K23)_PCR/3274 [Gene]SCO0119/2980 NC_003888 SCJ11.48c, possible small secreted protein, len: 173aa; contains possible N-terminal signal sequence. 3 2 12 8 [Reporter]SCO0556 (14G23)_PCR/3273 [Gene]SCO0556/2979 NC_003888 SCF73.03c, unknown, len: 507 aa 3 2 12 7 [Reporter]SCO0366 (15C23)_PCR/3272 [Gene]SCO0366/2978 NC_003888 SCF41.25, hypothetical protein, len: 355 aa; similar to the plasmid borne TR:Q9R6D3 (EMBL:AB016260) Agrobacterium radiobacter TIORF127 protein, 361 aa; fasta scores: opt: 520 Z-score: 612.2 E(): 1.8e-26; 34.295% identity in 312 aa overlap 3 2 12 6 [Reporter]SCO0349 (16O19)_PCR/3271 [Gene]SCO0349/2977 NC_003888 SCF41.08, possible oxidoreductase (putative secreted protein), len: 334 aa. Similar to Pseudomonas sp. TR:Q52472 (EMBL; D32042) L-fucose dehydrogenase (329 aa), fasta scores opt: 960 z-score: 1078.8 E(): 0 48.4% identity in 318 aa overlap 3 2 12 5 [Reporter]SCO5164 (17K19)_PCR/3270 [Gene]SCO5164/2976 NC_003888 SCP8.27, hypothetical protein, len: 300 aa; similar to N-terminal region of TR:Q9S2S7 (EMBL:AL096884) Streptomyces coelicolor hypothetical 44.0 kD protein SC4G6.31c, 403 aa; fasta scores: opt: 423 z-score: 494.8 E(): 4.3e-20; 36.5% identity in 230 aa overlap 3 2 12 3 [Reporter]SCO6277 (19C19)_PCR/3268 [Gene]SCO6277/2975 NC_003888 SC1G7.03, possible epoxide hydrolase, len: 286 aa; similar to TR:Q42566 (EMBL:D16628) Arabidopsis thaliana epoxide hydrolase (EC 3.3.2.3) Seh or F18A8.11, 321 aa; fasta scores: opt: 351 Z-score: 406.4 bits: 83.3 E(): 4.8e-15; 29.560% identity in 318 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 3 2 12 2 [Reporter]SCO5064 (20K15)_PCR/3267 [Gene]SCO5064/2974 NC_003888 SCBAC20F6.07, possible bifunctional protein, len: 800 aa; N-terminal domain does not present similarity to any known protein and C-terminal half is similar to the C-terminal domain of may prokaryotic putative helicases, e.g. TR:Q9CE08 (EMBL:AE006434) Lactococcus lactis (subsp. lactis) SWI/SNF family helicase Snf, 1034 aa; fasta scores: opt: 317 Z-score: 332.6 bits: 72.8 E(): 6.1e-11; 29.817% identity in 436 aa overlap. Contains Pfam match to entry PF00176 SNF2_N, SNF2 and others N-terminal domain and Pfam match to entry PF00271 helicase_C, Helicase conserved C-terminal domain 2 4 7 13 [Reporter]SCO7172 (9F21)_PCR/6695 [Gene]SCO7172/6099 NC_003888 SC9A4.34, possible membrane protein, len: 78 aa. Contains possible hydrophobic membrane spanning regions 3 2 11 22 [Reporter]SCO1263 (1K11)_PCR/3265 [Gene]SCO1263/2973 NC_003888 2SCG18.10c, probable ECF-sigma factor, len: 295 aa; similar to TR:Q9RDI5 (EMBL:AL136519) Streptomyces coelicolor putative sigma factor SCC57A.13c, 315 aa; fasta scores: opt: 887 z-score: 1027.2 E(): 0; 50.5% identity in 285 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 2 4 7 12 [Reporter]SCO2310 (10B21)_PCR/6694 [Gene]SCO2310/6098 NC_003888 SCC53.01, possible integral membrane efflux protein, partial CDS, len: 277aa; similar to C-terminus of many eg. TR:Q9ZBJ1 (EMBL:AL035161) putative efflux protein from Streptomyces coelicolor (815 aa) fasta scores; opt: 249, z-score: 264.8, E(): 2.8e-07, 29.9% identity in 314 aa overlap and SW:P39886 (TCMA_STRGA) tetracenomycin c resistance and export protein from Streptomyces glaucescens (538 aa) fasta scores; opt: 197, z-score: 213.0, E(): 0.00022, 30.1% identity in 176 aa overlap. Contains possible membrane-spanning hydrophobic regions.,SCC30.18, probable transport protein (fragment), len: >348 aa; similar to SW:TCMA_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C resistance and export protein TcmA, 538 aa; fasta scores: opt: 447 z-score: 504.9 E(): 1.1e-20; 27.7% identity in 361 aa overlap. Contains possible hydrophobic membrane spanning regions 3 2 11 21 [Reporter]SCO5212 (2G11)_PCR/3264 [Gene]SCO5212/2972 NC_003888 SC7E4.09c, aroA2, 3-phosphoshikimate 1-carboxyvinyltransferase, len: 438 aa; highly similar to SW:AROA_MYCTU (EMBL:X52269) Mycobacterium tuberculosis 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) AroA, 450 aa; fasta scores: opt: 1236 z-score: 1339.4 E(): 0; 52.1% identity in 434 aa overlap and similar to TR:CAB71266 (EMBL:AL138598) Streptomyces coelicolor SC1A2.28c AroA, 440a aa; fasta scores: opt: 731 z-score: 673.3 E(): 6.4e-32; 36.3% identity in 413 aa overlap. Contains Pfam match to entry PF00275 EPSP_syntase, EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) 2 4 7 11 [Reporter]SCO4526 (11N17)_PCR/6693 [Gene]SCO4526/6097 NC_003888 SCD35.33, possible protein kinase, len: 435 aa; similar to TR:P94647 (EMBL:U77780) Chlorobium limicola secretory protein kinase Kbh, 474 aa; fasta scores: opt: 722 z-score: 805.0 E(): 0; 35.9% identity in 368 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 2 11 20 [Reporter]SCO1486 (3C11)_PCR/3263 [Gene]SCO1486/2971 NC_003888 SC9C5.10c, pyrC, dihydroorotase, len: 428 aa; similar to SW:PYRC_LACLE (EMBL:X78999) Lactobacillus leichmannii dihydroorotase (EC 3.5.2.3) PyrC, 427 aa; fasta scores: opt: 988 z-score: 1126.5 E(): 0; 39.6% identity in 422 aa overlap. Contains Pfam match to entry PF00744 Dihydrooratase, Dihydroorotase-like and match to Prosite entry PS00483 Dihydroorotase signature 2 2 4 7 10 [Reporter]SCO2908 (12J17)_PCR/6692 [Gene]SCO2908/6096 NC_003888 SCE19A.08c, conserved hypothetical protein, len: 250 aa; unknown function, similar to hypothetical proteins from Mycobacterium leprae, Mycobacterium tuberculosis and bacillus subtilis e.g. SW:Y03O_MYCLE (EMBL:U00014) Mycobacterium leprae hypothetical protein (284 aa), fasta scores; opt: 642 z-score: 777.3 E(): 0, 43.0% identity in 256 aa overlap. Similar to SC66T3.08c (EMBL:AL079348) S.coelicolor hypothetical protein (270 aa) (36.4% identity in 272 aa overlap) 3 2 11 19 [Reporter]SCO2250 (4O7)_PCR/3262 [Gene]SCO2250/2970 NC_003888 SC1G2.12, unknown, len: 275 aa. Weakly similar to a protein of undefined function from Mycobacterium tuberculosis TR:O07720(EMBL:Z97193) hypothetical 26.9 KD protein (250 aa), fasta scores 0pt: 176 z-score: 214.1 E(): 0.00015 28.4% identity in 250 aa overlap. 2 4 7 9 [Reporter]SCO0199 (13F17)_PCR/6691 [Gene]SCO0199/6095 NC_003888 SCJ12.11c, probable alcohol dehydrogenase, len: 340 aa. Highly similar to many e.g. Bacillus stearothermophilus SW:ADH3_BACST (EMBL; Z27089) alcohol dehydrogenase (EC 1.1.1.1) (ADH-HT) (339 aa), fasta scores opt: 1281 z-score: 1383.7 E(): 0 55.2% identity in 337 aa overlap. Contains a PS00059 Zinc-containing alcohol dehydrogenases signature and a Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases. 2 4 7 8 [Reporter]SCO0133 (14B17)_PCR/6690 [Gene]SCO0133/6094 NC_003888 SCJ21.14c, unknown, len: 126 aa 2 4 7 7 [Reporter]SCO3688 (15N13)_PCR/6689 [Gene]SCO3688/6093 NC_003888 SCH35.36c, hypothetical protein, len: 320 aa; glutamic acid rich, similar to TR:CAA22041 (EMBL:AL033505) hypothetical protein from Streptomyces coelicolor (376 aa) fasta scores; opt: 1014, z-score: 1002.4, E(): 0, (51.1% identity in 323 aa overlap) 2 4 7 6 [Reporter]SCO1532 (16J13)_PCR/6688 [Gene]SCO1532/6092 NC_003888 SCL2.22c, hypothetical protein, len: 425 aa; similar to TR:Q9X4H6 (EMBL:AF110772) Streptomyces aureofaciens putative aldehyde dehydrogenase AldA, 467 aa; fasta scores: opt: 156 z-score: 162.9 E(): 0.11; 29.0% identity in 328 aa overlap 2 4 7 5 [Reporter]SCO0352 (17F13)_PCR/6687 [Gene]SCO0352/6091 NC_003888 SCF41.11 possible solute-binding protein, len: 422 aa. Similar to many including: Streptomyces lividans TR:Q9Z492 (EMBL; AF043654) BxlE precursor (434 aa), fasta scores opt: 287 z-score: 311.3 E(): 5.5e-10 25.7% identity in 354 aa overlap. Contains a PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. 2 4 7 4 [Reporter]SCO1783 (18B13)_PCR/6686 [Gene]SCO1783/6090 NC_003888 SCI51.23c, hypothetical protein, len: 115 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. The C-terminus is Pro-rich 3 2 11 18 [Reporter]SCO2204 (5K7)_PCR/3261 [Gene]SCO2204/2969 NC_003888 SC3H12.12, hypothetical protein, len: 395 aa; identical to N-terminal region of previously sequenced TR:CAB58286 (EMBL:AL121850) Streptomyces coelicolor hypothetical 37.4 kD protein (fragment) SCC78.05, 347 aa 3 2 11 17 [Reporter]SCO2054 (6G7)_PCR/3260 [Gene]SCO2054/2968 NC_003888 SC4G6.23c, hisD, histidinol dehydrogenase, len: 441aa; previously sequenced therefore identical to SW:HISX_STRCO. Contains Pfam match to entry PF00815 Histidinol_dh, Histidinol dehydrogenase and Prosite match to PS00611 Histidinol dehydrogenase signature. 3 2 11 16 [Reporter]SCO7267 (7C7)_PCR/3259 [Gene]SCO7267/2967 NC_003888 SC5H1.25c, conserved hypothetical protein, len: 131 aa; similar to e.g. SW:YFC3_SHEPU (EMBL:L04283) Shewanella putrefaciens hypothetical protein (118 aa), fasta scores; opt: 372 z-score: 466.1 E(): 1.2e-18, 52.2% identity in 115 aa overlap. Weakly similar to some lyases e.g. SW:LGUL_SALTY (EMBL:U57364), gloA, Salmonella typhimurium lactoylglutathione lyase (135 aa) (23.6% identity in 127 aa overlap). Also similar to TR:O86701 (EMBL:AL031515) S.coelicolor possible lyase (138 aa) (33.1% identity in 127 aa overlap) 3 2 11 15 [Reporter]SCO5580 (8O3)_PCR/3258 [Gene]SCO5580/2966 NC_003888 SC7A1.24, ftsY, prokaryotic docking protein, len: 412aa; similar to many including SW:FTSY_ECOLI functional homolog of the mammalian SRP receptor (docking protein) from Escherichia coli (497 aa) fasta scores; opt: 885, z-score: 906.8, E(): 0, (40.7% identity in 388 aa overlap). Contains PS00017 ATP /GTP-binding site motif A (P-loop), PS00300 SRP54-type proteins GTP-binding domain signature and Pfam match to entry PF00448 SRP54, SRP54-type protein, score 323.90, E-value 1.8e-93. Contains possible hydrophobic membrane spanning region 3 2 11 14 [Reporter]SCO7806 (9K3)_PCR/3257 [Gene]SCO7806/2965 NC_003888 SC8E7.03c, possible DNA-binding protein, len: 243 aa. Weakly similar to several including: Streptomyces ambofaciens protein of unknown function found within the SrmR regulatory locus TR:Q00510 (EMBL:X63451) SrmX (239 aa), fasta scores opt: 167 z-score: 217.0 E(): 0.00013 30.9% identity in 220 aa overlap and Deinococcus radiodurans TR:Q9RTS7 (EMBL:AE002009) N-methyl-transferase-related protein (249 aa), fasta scores opt: 160 z-score: 208.0 E(): 0.00041 27.1% identity in 214 aa overlap. Contains a putative helix-turn-helix motif situated between residues 60..81 (+3.06 SD). 3 2 11 13 [Reporter]SCO5120 (10G3)_PCR/3256 [Gene]SCO5120/2964 NC_003888 SC9E12.05, probable peptide transport system ATP-binding protein, len: 327 aa; similar to SW:OPPD_BACSU (EMBL:X56347) Bacillus subtilis oligopeptide transport system ATP-binding protein OppD, 358 aa; fasta scores: opt: 1126 z-score: 1167.7 E(): 0; 53.9% identity in 310 aa overlap, to TR:P72409 (EMBL:U68036) Streptomyces coelicolor putative ABC transporter intracellular ATPase subunit BldKD, 353 aa; fasta scores: opt: 1035 z-score: 1074.2 E(): 0; 51.5% identity in 326 aa overlap and to TR:O86574 (EMBL:AL031184) Streptomyces coelicolor oligopeptide ABC transporter ATP-binding protein SC2A11.13, 365 aa; fasta scores: opt: 1097 z-score: 945.2 E(): 0; 52.3% identity in 331 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 2 4 7 3 [Reporter]SCO4997 (19N9)_PCR/6685 [Gene]SCO4997/6089 NC_003888 2SCK36.20c, hypothetical protein, len: 63 aa; similar to TR:Q9F3E1 (EMBL:AL450289) Streptomyces coelicolor putative AbaA-like protein SC2H2.14, 89 aa; fasta scores: opt: 96 Z-score: 150.9 bits: 31.9 E(): 0.86; 32.787% identity in 61 aa overlap 3 2 11 12 [Reporter]SCO2260 (11C3)_PCR/3255 [Gene]SCO2260/2963 NC_003888 SCC75A.06, possible membrane protein, len: 265 aa. Highly similar to a protein of undefined function from Mycobacterium tuberculosis TR:O06309(EMBL:Z95324) (259 aa), fasta scores opt: 646 z-score: 795.3 E():0 42.9% identity in 259 aa overlap. Contains a possible N-terminal signal sequence and multiple possible membrane spanning hydrophobic domains. 2 4 7 2 [Reporter]SCO7461 (20F9)_PCR/6684 [Gene]SCO7461/6088 NC_003888 SCBAC14E8.01c, probable hydrolase, len: 228 aa; similar to SW:TRYD_DROME (EMBL:U04853) Drosophila melanogaster trypsin delta precursor (EC 3.4.21.4) Try-delta, 253aa; fasta scores: opt: 220 Z-score: 237.9 bits: 51.4 E(): 1.1e-05; 32.271% identity in 251 aa overlap. Contains Pfam match to entry PF00089 trypsin, Trypsin 3 2 11 11 [Reporter]SCO4435 (11O23)_PCR/3254 [Gene]SCO4435/2962 NC_003888 SCD6.13, unknown, len: 371 aa. High content in alanine, glycine and leucine amino acid residues and also high G+C content (78.43%) 2 4 6 22 [Reporter]SCO5382 (1F5)_PCR/6682 [Gene]SCO5382/6087 NC_003888 2SC6G5.26c, possible ABC transporter, integral membrane subunit, len: 249aa; similar to TR:Q53627 (EMBL:U43537) membrane protein involved in mithramycin resistance from Streptomyces argillaceus (233 aa) fasta scores; opt: 704, z-score: 811.8, E(): 0, 47.1% identity in 227 aa overlap. Contains Pfam match to entry PF01061 ABC2_membrane, ABC-2 type transporter and possible membrane-spanning hydrophobic regions. 3 2 11 9 [Reporter]SCO2875 (13G23)_PCR/3252 [Gene]SCO2875/2961 NC_003888 SCE6.12, possible MerR-family transcriptional regulator, len: 135 aa. Similar to many regulators e.g. Haemophilus influenzae SW:Y186_HAEIN(EMBL:U32704) hypothetical transcriptional regulator (135 aa), fasta scores opt: 233 z-score: 315.6 E(): 3.8e-10 33.9% identity in 121 aa overlap. Also similar to Streptomyces coelicolor TR:CAB62710(EMBL:AL133423) putative MerR-family transcriptional regulator, SC4A7.06C (196 aa), fasta scores opt: 295 z-score: 393.5 E(): 1.7e-14 41.2% identity in 119 aa overlap. Contains a Prosite hit to PS00552 Bacterial regulatory proteins, merR family signature and a Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family with a putative helix-turn-helix motif situated between residues 6..27 (+4.68 SD). 2 4 6 21 [Reporter]SCO0779 (2B5)_PCR/6681 [Gene]SCO0779/6086 NC_003888 3SCF60.11c, conserved hypothetical protein, len: 131 aa; identical to TR:O85701 (EMBL:AF072709) Streptomyces lividans hypothetical 14.2 kDa protein, 131 aa and similar to TR:Q9L0J1 (EMBL:AL161691) Streptomyces coelicolor hypothetical 15.7 kDa protein SCD40A.20, 145 aa; fasta scores: opt: 179 z-score: 233.2 E(): 1.8e-05; 31.8% identity in 110 aa overlap 3 2 11 8 [Reporter]SCO1539 (14C23)_PCR/3251 [Gene]SCO1539/2960 NC_003888 SCL2.29c, possible secreted protein, len: 433 aa; similar to TR:O31518 (EMBL:Z99107) Bacillus subtilis YesO protein, 412 aa; fasta scores: opt: 606 z-score: 680.7 E(): 1.7e-30; 27.7% identity in 411 aa overlap and to TR:CAB59595 (EMBL:AL132662) Streptomyces coelicolor probable sugar transporter sugar binding lipoprotein SCF11.18, 442 aa; fasta scores: opt: 312 z-score: 352.1 E(): 3.4e-12; 24.4% identity in 454 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains N-terminal region signal peptide sequence 2 4 6 20 [Reporter]SCO3252 (3N1)_PCR/6680 [Gene]SCO3252/6085 NC_003888 SCE39.02c, repSA, replication initiator protein, len: 466 aa; highly similar to TR:E1288125 (EMBL:AJ005260) RepSA, replication initiator protein from the integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens (459 aa) fasta scores; opt: 2822, z-score: 3195.3, E(): 0, (89.5% identity in 455 aa overlap) and TR:E1355655 (EMBL:AL034355) putative plasmid replication initiator protein from Streptomyces coelicolor (452 aa) fasta scores; opt: 732, z-score: 831.1, E(): 0, (45.8% identity in 459 aa overlap). 2 4 6 19 [Reporter]SCO6033 (4J1)_PCR/6679 [Gene]SCO6033/6084 NC_003888 SC1C3.21, unknown, len: 364 aa 2 4 6 18 [Reporter]SCO7098 (5F1)_PCR/6678 [Gene]SCO7098/6083 NC_003888 SC3A4.24c, unknown, len: 178 aa. Weakly similar to another hypothetical protein from Streptomyces coelicolor: TR:CAB52889(EMBL:AL109949) SCJ11.04 (195 aa), fasta scores opt: 122 z-score: 143.6 E(): 1.5 29.2% identity in 154 aa overlap. Contains a possible coiled-coil region between residues 28..57. 2 4 6 17 [Reporter]SCO7329 (6B1)_PCR/6677 [Gene]SCO7329/6082 NC_003888 SC4G10.08c, possible long-chain-fatty-acid-CoA ligase, len: 636 aa. Highly similar to many proposed long-chain-fatty-acid-CoA ligases including: Homo sapiens SW:LCFB_HUMAN(EMBL:D10040) long-chain-fatty-acid--CoA ligase 2 (EC 6.2.1.3) (698 aa), fasta scores opt: 447 z-score: 498.5 E(): 2.6e-20 26.3% identity in 616 aa overlap and several from Streptomyces coelicolor e.g. TR:Q9ZBW6(EMBL:AL034443) SC4B5.02C (608 aa), fasta scores opt: 1881 z-score: 2101.5 E(): 0 47.0% identity in 611 aa overlap. Contains a Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme. 2 4 6 16 [Reporter]SCO7317 (6N21)_PCR/6676 [Gene]SCO7317/6081 NC_003888 SC5F8.27, unknown, len: 144 aa. 2 4 6 15 [Reporter]SCO6953 (7J21)_PCR/6675 [Gene]SCO6953/6080 NC_003888 SC6F7.06c, unknown, len: 525 aa; low similarity to Streptomyces coelicolor SCP1 plasmid borne TR:Q9ACU9 (EMBL:AL590464) Streptomyces coelicolor hypothetical protein SCP1.204c, 553 aa; fasta scores: opt: 617 Z-score: 687.1 E(): 1.2e-30; 29.916% identity in 478 aa overlap 3 2 11 7 [Reporter]SCO3408 (15O19)_PCR/3250 [Gene]SCO3408/2959 NC_003888 SCE9.15c, possible penicillin-binding protein, len: 459 aa; weakly similar to penicillin-binding proteins e.g. SW:DAC_ACTSP (EMBL:X64790), dac, Actinomadura sp. D-alanyl-D-alanine carboxypeptidase precursor (538 aa), fasta scores; opt: 248 z-score: 243.2 E(): 3.2e-06, 29.5% identity in 499 aa overlap and TR:O85665 (EMBL:AF071224), pbp3, Neisseria gonorrhoeae penicillin binding protein 3 (469 aa) (26.8% identity in 399 aa overlap). Similar to SC9B2.18c (EMBL:AL035212) S.coelicolor probable carboxypeptidase (451 aa) (36.1% identity in 429 aa overlap). The start codon is uncertain as there are 2 potential 3 2 11 6 [Reporter]SCO0396 (16K19)_PCR/3249 [Gene]SCO0396/2958 NC_003888 SCF62.22, unknown, len: 424 aa 3 2 11 5 [Reporter]SCO3649 (17G19)_PCR/3248 [Gene]SCO3649/2957 NC_003888 SCH10.27c, fba, probable fructose 1,6-bisphosphate aldolase, len: 343 aa; similar to many e.g. SW:ALF_STRGB (EMBL:AJ131707) Streptomyces galbus fructose 1,6-bisphosphate aldolase Fba (340 aa), fasta scores: opt: 1966 Z-score: 2152.8 E(): 2.9e-112; 87.464% identity in 343 aa overlap. Contains Pfam match to entry PF01116 F_bP_aldolase, Fructose-bisphosphate aldolase class-II, score 426.40, E-value 2.5e-124 3 2 11 4 [Reporter]SCO0547 (18C19)_PCR/3247 [Gene]SCO0547/2956 NC_003888 SCF11.27c, possible acyltransferase, len: 333 aa; similar to TR:CAB52353 (EMBL:AL109747) Streptomyces coelicolor putative multi-domain beta keto-acyl synthase, 2082 aa; fasta scores: opt: 402 z-score: 416.4 E(): 8e-16; 32.5% identity in 329 aa overlap, and to TR:Q55226 (EMBL:L34880) Streptomyces sp. acyltransferase DauA, 338 aa; fasta scores: opt: 326 z-score: 349.0 E(): 4.5e-12; 33.4% identity in 326 aa overlap. Contains Pfam match to entry PF00698 acyl_transf, acyl transferase domain 3 2 11 3 [Reporter]SCO6325 (19O15)_PCR/3246 [Gene]SCO6325/2955 NC_003888 SC10H5.01c, unknown, partial CDS, len >109 aa; possible integral membrane protein,SCIF3.27c, possible membrane protein (fragment), len: >221 aa; similar to TR:Q9ZC02 (EMBL:AL033505) Streptomyces coelicolor hypothetical 52.3 kDa protein SC1E6.20c, 501 aa; fasta scores: opt: 245 z-score: 288.4 E(): 1.6e-08; 36.3% identity in 212 aa overlap 3 2 11 2 [Reporter]SCO7481 (20G15)_PCR/3245 [Gene]SCO7481/2954 NC_003888 SCBAC17A6.14c, conserved hypothetical protein, len: 191aa: similar to many eg. TR:Q9F2L4 (EMBL:AL442629) hypothetical protein from Streptomyces coelicolor (151 aa) fasta scores; opt: 588, Z-score: 595.0, 65.185% identity (65.185% ungapped) in 135 aa overlap and TR:Q52355 (EMBL:U59239) TerB tellurite resistance protein from Serratia marcescens plasmid R478 (151 aa) fasta scores; opt: 240, Z-score: 254.3, 28.467% identity (28.467% ungapped) in 137 aa overlap 2 4 6 14 [Reporter]SCO6890 (8F21)_PCR/6674 [Gene]SCO6890/6079 NC_003888 SC7F9.42, possible secreted protein, len: 149 aa. Contains a possible N-terminal signal sequence. 3 2 10 22 [Reporter]SCO2801 (1G11)_PCR/3243 [Gene]SCO2801/2953 NC_003888 2SCC13.09c, probable two component system response regulator, len: 234 aa; similar to many other Streptomyces coelicolor two component system response regulators, e.g. TR:Q9RK58 (EMBL:AL132662) putative response regulator SCF11.32c, 238 aa; fasta scores: opt: 894 z-score: 1031.1 E(): 0; 60.6% identity in 226 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 2 4 6 13 [Reporter]SCO7168 (9B21)_PCR/6673 [Gene]SCO7168/6078 NC_003888 SC9A4.30, probable gntR-family transcriptional regulator, len: 224 aa; similar to TR:Q9XA67 (EMBL:AL096822) Streptomyces coelicolor putative gntR-family transcriptional regulator SCGD3.11c, 216 aa; fasta scores: opt: 305 z-score: 351.3 E(): 4.2e-12; 32.7% identity in 196 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. Also contains possible helix-turn-helix motif at residues 36..57 (+3.11 SD) 3 2 10 21 [Reporter]SCO1570 (2C11)_PCR/3242 [Gene]SCO1570/2952 NC_003888 SCL24.06c, argH, argininosuccinate lyase, len: 475 aa; highly similar to SW:ARLY_CORGL (EMBL:AF049897) Corynebacterium glutamicum argininosuccinate lyase (EC 4.3.2.1) ArgH, 478 aa; fasta scores: opt: 1473 z-score: 1658.6 E(): 0; 59.1% identity in 391 aa overlap. Contains Pfam match to entry PF00206 lyase_1, Lyase and two matches to Prosite entries PS00163 Fumarate lyases signature and PS00017 ATP/GTP-binding site motif A (P-loop) 2 4 6 12 [Reporter]SCO1954 (10N17)_PCR/6672 [Gene]SCO1954/6077 NC_003888 SCC54.14, unknown, len: 316aa; 3 2 10 20 [Reporter]SCO4711 (3O7)_PCR/3241 [Gene]SCO4711/2951 NC_003888 SCD31.36, rpsQ, 30S ribosomal protein S17, len: 95 aa; highly similar to SW:RS17_BACSU (EMBL:X15664) Bacillus subtilis 30S ribosomal protein S17 RpsQ, 86 aa; fasta scores: opt: 316 z-score: 456.1 E(): 5.7e-18; 61.7% identity in 81 aa overlap. Contains Pfam match to entry PF00366 Ribosomal_S17, Ribosomal protein S17 and match to Prosite entry PS00056 Ribosomal protein S17 signature 2 4 6 11 [Reporter]SCO4522 (11J17)_PCR/6671 [Gene]SCO4522/6076 NC_003888 SCD35.29c, unknown, len: 158 aa 3 2 10 18 [Reporter]SCO5734 (5G7)_PCR/3239 [Gene]SCO5734/2950 NC_003888 SC3C3.20c, unknown ATP/GTP binding protein (putative membrane protein), len: 1321 aa; similar to several in M. tuberculosis and M. leprae e.g. M. tuberculosis TR:O06264 (EMBL:Z95389) MTCY77.19c, Rv3447c (1236 aa), fasta scores; opt: 1186 z-score: 3117.9 E(): 0, 42.9% identity in 1312 aa overlap. Contains 3x, PS00017 ATP /GTP-binding site motif A (P-loop), and a possible transmembrane domain near the C-terminus 2 4 6 10 [Reporter]SCO4280 (12F17)_PCR/6670 [Gene]SCO4280/6075 NC_003888 SCD95A.13c, probable reductase, len: 202 aa; similar to SW:DIM6_STRCO (EMBL:X63449) Streptomyces coelicolor actinorhidin polyketide dimerase ActVB, 177 aa; fasta scores: opt: 256 z-score: 298.4 E(): 3.7e-09; 37.3% identity in 166 aa overlap. Contains Pfam match to entry PF01613 Flavin_Reduct, Flavin reductase like domain 2 4 6 9 [Reporter]SCO0412 (13B17)_PCR/6669 [Gene]SCO0412/6074 NC_003888 SCF51.11, unknown, len: 127 aa 2 4 6 8 [Reporter]SCO0955 (14N13)_PCR/6668 [Gene]SCO0955/6073 NC_003888 SCM11.10c, unknown, len: 58 aa 2 4 6 6 [Reporter]SCO0913 (16F13)_PCR/6666 [Gene]SCO0913/6072 NC_003888 SCM10.01, hypothetical protein (partial CDS), len: >115 aa; similar to various hypothetical proteins, e.g. TR:O69669 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical 35.4 kD protein MTV025.049c, 321 aa; fasta scores: opt: 413 z-score: 559.6 E(): 8.8e-24; 59.8% identity in 112 aa overlap,SCM1.46, hypothetical protein, partial CDS, len: > 251 aa. Similar to several proteins of undefined function including: Mycobacterium tuberculosis TR:O69669(EMBL:AL022121) hypothetical 35.4 KD protein (321 aa), fasta scores opt: 897 z-score: 1053.0 E():0 59.9% identity in 242 aa overlap and Synechocystis sp. (strain PCC 6803) TR:P73759(EMBL:D90909) hypothetical 38.0 KD protein (337 aa), fasta scores opt: 646 z-score:759.9 E():0 47.4% identity in 232 aa overlap. 2 4 6 5 [Reporter]SCO0919 (17B13)_PCR/6665 [Gene]SCO0919/6071 NC_003888 SCM10.07c, unknown, len: 319 aa. Contains glycine/alanine rich region 2 4 6 4 [Reporter]SCO3162 (18N9)_PCR/6664 [Gene]SCO3162/6070 NC_003888 SCE87.13, possible esterase, len: 420 aa. Similar to several including: Streptomyces chrysomallus TR:O87861(EMBL:Z15137) esterase A (389 aa), fasta scores opt: 684 z-score: 729.1 E():0 44.9% identity in 405 aa overlap and Pseudomonas fluorescens TR:Q53403 (EMBL:S69066) esterase III (382 aa), fasta scores opt: 597 z-score: 637.2 E(): 4e-28 41.8% identity in 397 aa overlap. 3 2 10 17 [Reporter]SCO2050 (6C7)_PCR/3238 [Gene]SCO2050/2949 NC_003888 SC4G6.19c, hisA, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, len: 240aa; previously sequenced therefore identical to SW:HIS4_STRCO. Contains Pfam match to entry PF00977 His_biosynth, Histidine biosynthesis protein. 3 2 10 16 [Reporter]SCO3616 (7O3)_PCR/3237 [Gene]SCO3616/2948 NC_003888 SC66T3.27, hypothetical protein, len: 72 aa; unknown function, possible CDS suggested by positional base preference and GC frame analysis 3 2 10 15 [Reporter]SCO5576 (8K3)_PCR/3236 [Gene]SCO5576/2947 NC_003888 SC7A1.20, acy, possible acylphosphatase, len: 93aa; similar to SW:ACYP_MYCTU putative acylphosphatase from Mycobacterium tuberculosis (93 aa) opt: 345, z-score: 576.5, E(): 8.2e-25, (58.9% identity in 90 aa overlap) and SW:ACYO_CHICK acylphosphatase from chicken (Gallus gallus) (98 aa) opt: 150, z-score: 208.0, E(): 0.00027, (45.0% identity in 60 aa overlap). Contains PS00150 Acylphosphatase signature 1. While acylphosphatases have been so far only characterized in vertebrates, there are a number of bacterial and archebacterial hypothetical proteins that are highly similar to that enzyme and that probably possess the same activity. 3 2 10 14 [Reporter]SCO5667 (9G3)_PCR/3235 [Gene]SCO5667/2946 NC_003888 SC8B7.11c, probable ABC-transporter polyamine-binding lipoprotein, len: 399 aa; similar to e.g. POTF_ECOLI putrescine-binding periplasmic protein precursor (370 aa), fasta scores; opt: 327 z-score: 259.5 E(): 3.4e-07, 28.5% identity in 365 aa overlap. Also similar to upstream gene SC6A9.09c (E(): 0, 46.8% identity in 391 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site and PS00017 ATP/GTP-binding site motif A (P-loop). Contains possible N-terminal region signal sequence peptide 3 2 10 13 [Reporter]SCO6492 (10C3)_PCR/3234 [Gene]SCO6492/2945 NC_003888 SC1E6.01, unknown, partial CDS, len: >616 aa, some similarity to hypothetical protein YPHG_ECOLI (1124 aa), fasta scores; opt: 360 z-score: 279.9 E(): 2.6e-08, 27.1% identity in 310 aa overlap,SC9C7.28, partial CDS, unknown, len: 83 aa; constitutes the N-terminus of TR:E1341375 (EMBL:AL033505) hypothetical protein from Streptomyces coelicolor. 3 2 10 12 [Reporter]SCO2686 (10O23)_PCR/3233 [Gene]SCO2686/2944 NC_003888 SCC61A.07c, possible luxR-family transcriptional regulator, len: 338aa; similar to many eg. TR:Q56084 (EMBL:D63904) erythropoiesis-stimulating protein from Streptomyces thermoviolaceus (334 aa) fasta scores; opt: 393, z-score: 460.3, E(): 3.5e-18, 32.5% identity in 295 aa overlap and SW:Q01108 (BRPA_STRHY) bialaphos biosynthetic pathway regulatory protein from Streptomyces hygroscopicus (256 aa) fasta scores; opt: 178, z-score: 213.5, E(): 0.0002, 25.1% identity in 255 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and helix-turn-helix motif 276-297aa. 2 4 6 3 [Reporter]SCO4900 (19J9)_PCR/6663 [Gene]SCO4900/6069 NC_003888 2SCK8.26c, hypothetical protein, len: 253 aa; similar to TR:Q9RD48 (EMBL:AL133424) Streptomyces coelicolor hypothetical 31.2 kDa protein SCF56.19, 297 aa; fasta scores: opt: 376 z-score: 449.5 E(): 1.8e-17; 36.4% identity in 253 aa overlap 3 2 10 11 [Reporter]SCO4431 (11K23)_PCR/3232 [Gene]SCO4431/2943 NC_003888 SCD6.09c, possible integral membrane protein, len: 169 aa. Contains possible N-terminal region signal peptide sequence and possible hydrophobic membrane spanning regions 2 4 6 2 [Reporter]SCP1.265c (20B9)_PCR/6662 [Gene]SCP1.265c/6068 NC_003888 SCP1.265c, unknown, len: 229aa; 3 2 10 10 [Reporter]SCO3501 (12G23)_PCR/3231 [Gene]SCO3501/2942 NC_003888 SCE134.02c, unknown, len: 561aa; 2 4 5 22 [Reporter]SCO5375 (1B5)_PCR/6660 [Gene]SCO5375/6067 NC_003888 2SC6G5.19, possible secreted protein, len: 151aa; similar to SW:Q10620 (YD12_MYCTU) hypothetical protein from Mycobacterium tuberculosis (147 aa) fasta scores; opt: 282, z-score: 358.4, E(): 1.7e-12, 38.9% identity in 144 aa overlap. Contains possible N-terminal signal sequence peptide 3 2 10 9 [Reporter]SCO1030 (13C23)_PCR/3230 [Gene]SCO1030/2941 NC_003888 SCG20A.10, conserved hypothetical protein, len: 172 aa; similar to TR:CAB88805 (EMBL:AL35383) Streptomyces coelicolor hypothetical 20.2 kD protein (fragment) SCE6.01c, 183 aa; fasta scores: opt: 227 z-score: 278.3 E(): 5e-08; 36.6% identity in 134 aa overlap 2 4 5 21 [Reporter]SCO5369 (2N1)_PCR/6659 [Gene]SCO5369/6066 NC_003888 2SC6G5.13, atpF, ATP synthase B chain (EC 3.6.1.34), len: 184aa; similar to many eg. SW:P00859 (ATPF_ECOLI) ATP synthase B chain from Escherichia coli (156 aa) fasta scores; opt: 246, z-score: 256.4, E(): 8.3e-07, 31.5% identity in 146 aa overlap. Almost identical to SW:P50013 (ATPF_STRLI) ATP synthase B chain from Streptomyces lividans (181 aa) fasta scores; opt: 1092, z-score: 1068.3, E(): 0, 97.3% identity in 184 aa overlap. Contains Pfam match to entry PF00430 ATP-synt_B, ATP synthase B/B', possible membrane-spanning hydrophobic regions and coiled-coil region 55-95aa. Contains possible hydrophobic membrane spanning region 3 2 10 8 [Reporter]SCO0541 (14O19)_PCR/3229 [Gene]SCO0541/2940 NC_003888 SCF11.21, probable alpha-galactosidase, len: 441 aa; similar to SW:AGAL_BACSU (EMBL:AF008220) Bacillus subtilis alpha-galactosidase (EC 3.2.1.22) (Melibiase) MelA, 432 aa; fasta scores: opt: 1068 z-score: 1240.5 E(): 0; 39.3% identity in 427 aa overlap 2 4 5 20 [Reporter]SCO5878 (3J1)_PCR/6658 [Gene]SCO5878/6065 NC_003888 SC2E9.19, redX, polyketide synthase with two beta-ketoacyl synthase domains, len: 982 aa; similar to many eg. ERY3_SACER Q03133 erythronolide synthase, modules 5 and 6 (3170 aa), fasta scores; opt: 649 z-score: 586.3 E(): 1.9e-25, 29.2% identity in 1029 aa overlap. Contains 2 x Pfam match to entry ketoacyl-synt PF00109, Beta-ketoacyl synthases, scores 52.40 and 310.80 2 4 5 19 [Reporter]SCO6159 (4F1)_PCR/6657 [Gene]SCO6159/6064 NC_003888 SC1A9.23, possible gntR family transcriptional regulator, len: 273 aa; similar to TR:O69996 (EMBL:AL022374) WhiH, sporulation transcription factor from Streptomyces coelicolor (295 aa) fasta scores; opt: 139, z-score: 267.9, E(): 1.2e-07, (29.8% identity in 292 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family, score 30.50, E-value 7.1e-08. 2 4 5 18 [Reporter]SCO7093 (5B1)_PCR/6656 [Gene]SCO7093/6063 NC_003888 SC3A4.19, possible transcriptional regulator, len: 932 aa. Highly similar to several including Streptomyces albus TR:Q53582(EMBL:U03114) transcriptional activator of the LipA promoter, LipR (890 aa), fasta scores opt: 714 z-score: 762.7 E(): 0 30.3% identity in 922 aa overlap and Streptomyces coelicolor TR:CAB52359(EMBL:AL109747) probable transcriptional regulator, SCJ21.13 (919 aa), fasta scores opt: 2398 z-score: 2564.6 E(): 0 48.0% identity in 911 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop) and a Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family with the putative helix-turn-helix motif situated between residues 883..904 (+3.20 SD). 2 4 5 17 [Reporter]SCO7053 (5N21)_PCR/6655 [Gene]SCO7053/6062 NC_003888 SC4G1.19c, hypothetical protein, len: 139 aa; similar to TR:O33283 (EMBL:AL008967) Mycobacterium tuberculosis hypothetical 16.6 kD protein, 149 aa; fasta scores: opt: 118 z-score: 161.0 E(): 0.17; 34.2% identity in 79 aa overlap 2 4 5 16 [Reporter]SCO7312 (6J21)_PCR/6654 [Gene]SCO7312/6061 NC_003888 SC5F8.22c, unknown, len: 77 aa. 2 4 5 15 [Reporter]SCO7422 (7F21)_PCR/6653 [Gene]SCO7422/6060 NC_003888 SC6D11.18c, cvnA10, possible sensor histidine-kinase, len: 543 aa. Similar to several from Streptomyces coelicolor including: TR:CAB61282 (EMBL:AL132991) putative sensor kinase, SCF55.12c (575 aa), fasta scores opt: 969 z-score: 843.9 E(): 0 42.9% identity in 501 aa overlap and TR:CAB59483 (EMBL:AL132648) putative integral membrane protein, SCI41.13c (541 aa), fasta scores opt: 630 z-score: 552.6 E(): 2.3e-23 32.9% identity in 559 aa overlap. Contains a Pfam match to entry PF00512 signal, Histidine kinase and a possible hydrophobic membrane spanning regions 3 2 10 7 [Reporter]SCO3747 (15K19)_PCR/3228 [Gene]SCO3747/2939 NC_003888 SCH22A.25, possible integral membrane protein, len: 456 aa; similar to TR:O06407 (EMBL:Z95558) Mycobacterium tuberculosis hypothetical 47.7 kD protein MTCY25D10.20c, 449 aa; fasta scores: opt: 1164 z-score: 1309.6 E(): 0; 49.6% identity in 464 aa overlap. Contains possible hydrophobic membrane spanning regions 3 2 10 6 [Reporter]SCO7341 (16G19)_PCR/3227 [Gene]SCO7341/2938 NC_003888 SC4G10.20c, sigG, possible RNA polymerase secondary sigma factor, len: 263 aa. Previously sequenced region: Streptomyces coelicolor TR:O52313 (EMBL:AF036131) (263 aa). Highly similar to Streptomyces coelicolor SW:RPOF_STRCO (EMBL:L11648) RNA polymerase sigma-F factor (287 aa), fasta scores opt: 682 z-score: 820.1 E(): 0 49.2% identity in 240 aa overlap. Contains a Prosite hit to PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2 and a Pfam match to entry PF00140 sigma70, Sigma-70 factor. A possible helix-turn-helix motif situated between residues 227..248 (+6.87 SD). 3 2 10 5 [Reporter]SCO1682 (17C19)_PCR/3226 [Gene]SCO1682/2937 NC_003888 SCI30A.03, probable zinc-binding alcohol dehydrogenase, len: 358 aa; similar to many both prokaryote and eukaryote e.g. SW:DHSO_BACSU sorbitol dehydrogenase from Bacillus subtilis (352 aa) fasta scores; opt: 616, z-score: 638.0, E(): 3.3e-28, (32.7% identity in 352 aa overlap) and DHSO_SHEEP sorbitol dehydrogenase from Ovis aries (sheep) (354 aa) fasta scores; opt: 606, z-score: 627.7, E(): 1.2e-27, (34.6% identity in 344 aa overlap). Conatins Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases. 3 2 10 4 [Reporter]SCO4063 (18O15)_PCR/3225 [Gene]SCO4063/2936 NC_003888 2SCD60.29c, possible secreted protein, len: 118 aa. Contains possible N-terminal region signal peptide sequence. Contains a TTA leucine codon (12), possible target for bldA regulation 3 2 10 3 [Reporter]SCO4041 (19K15)_PCR/3224 [Gene]SCO4041/2935 NC_003888 2SCD60.07, probable uracil phosphoribosyltransferase, len: 209 aa; similar to SW:UPP_STRSL (EMBL:L07793) Streptococcus salivarius uracil phosphoribosyltransferase (EC 2.4.2.9) Upp, 209 aa; fasta scores: opt: 707 z-score: 853.1 E(): 0; 48.3% identity in 207 aa overlap. Contains Pfam match to entry PF00156 Pribosyltran, Phosphoribosyl transferase domain 3 2 10 2 [Reporter]SCO5068 (20C15)_PCR/3223 [Gene]SCO5068/2934 NC_003888 SCBAC20F6.11c, possible tetR-family transcriptional regulator, len: 199 aa; similar to many actinomycetes putative tetR-family transcriptional regulators, e.g. TR:Q9RJL5 (EMBL:AL121849) Streptomyces coelicolor putative tetR-family transcriptional regulator SCF6.16, 194 aa; fasta scores: opt: 419 Z-score: 483.9 bits: 96.4 E(): 2.3e-19; 38.095% identity in 189 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 36..57 (+4.64 SD) 2 4 5 14 [Reporter]SCO6886 (8B21)_PCR/6652 [Gene]SCO6886/6059 NC_003888 SC7F9.38, unknown, len: 211 aa. Highly similar to Streptomyces coelicolor TR:Q9X8A6 (EMBL:AL049645) hypothetical 21.3 KD protein, SCE2.11 (199 aa), fasta scores opt: 562 z-score: 585.7 E(): 3.3e-25 44.4% identity in 189 aa overlap 3 2 9 22 [Reporter]SCO2797 (1C11)_PCR/3221 [Gene]SCO2797/2933 NC_003888 2SCC13.05, probable cellobiose transport permease, len: 303 aa; highly similar to TR:Q9X9R5 (EMBL:AJ009797) Streptomyces reticuli permease CebG protein, 275 aa; fasta scores: opt: 1262 z-score: 1477.0 E(): 0; 67.8% identity in 276 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 2 4 5 13 [Reporter]SCO6135 (9N17)_PCR/6651 [Gene]SCO6135/6058 NC_003888 SC9B2.22c, putative membrane protein, len: 224 aa; similar to SW:Y00U_MYCTU hypothetical protein from Mycobacterium tuberculosis (250 aa) fasta scores; opt: 125, z-score: 145.6, E(): 0.8, (26.4% identity in 208 aa overlap). Contains 4x5 residue perfect direct repeat, QTSVA, in the N-terminal region. Contains possible hydrophobic membrane spanning regions 3 2 9 20 [Reporter]SCO4727 (3K7)_PCR/3219 [Gene]SCO4727/2932 NC_003888 SC6G4.05, rpsM, 30S ribosomal protein S13, len: 126 aa; highly similar to many e.g. RS13_BACSU 30S ribosomal protein S13 (BS14) (120 aa), fasta scores; opt: 537 z-score: 986.9 E(): 0, 65.3% identity in 121 aa overlap. Contains PS 00646 Ribosomal protein S13 signature and Pfam match to entry PF00416 S13, Ribosomal protein S13, score 186.70, E-value 3.8e-52 2 4 5 11 [Reporter]SCO4359 (11F17)_PCR/6649 [Gene]SCO4359/6057 NC_003888 SCD19.14, probable ABC transport system ATP-binding protein, len: 336 aa; similar to SW:DRRA_STRPE (EMBL:M73758) Streptomyces peucetius daunorubicin resistance ATP-binding protein DrrA, 330 aa; fasta scores: opt: 912 z-score: 964.7 E(): 0; 49.5% identity in 327 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 2 9 19 [Reporter]SCO6053 (4G7)_PCR/3218 [Gene]SCO6053/2931 NC_003888 SC1B5.13c, putative membrane protein, len: 262 aa; highly similar to many members of the band 7/mec2 family of membrane proteins e.g. BAN7_MOUSE P54116 mus musculus (mouse). erythrocyte band7 protein (stomatin) (284 aa), fasta scores; opt: 709 z-score: 1075.9 E(): 0, 47.1% identity in 223 aa overlap. Contains possible hydrophobic membrane spanning region 2 4 5 10 [Reporter]SCO4275 (12B17)_PCR/6648 [Gene]SCO4275/6056 NC_003888 SCD95A.08, probable histidine autokinase, len: 428 aa; highly similar to TR:CAA74719 (EMBL:Y14336) Streptomyces reticuli histidine autokinase SenS, 398 aa; fasta scores: opt: 1703 z-score: 1743.6 E(): 0; 68.7% identity in 396 aa overlap. Contains possible hydrophobic membrane spanning regions in N-terminal domain 3 2 9 18 [Reporter]SCO5730 (5C7)_PCR/3217 [Gene]SCO5730/2930 NC_003888 SC3C3.16c, unknown, len: 140 aa 2 4 5 9 [Reporter]SCO0468 (13N13)_PCR/6647 [Gene]SCO0468/6055 NC_003888 SCF76.08c, unknown, len: 325 aa. The N-terminus is highly similar to several PIRIN proteins e.g. Alcaligenes eutrophus TR: AAD21775 (EMBL; J05278) PIRIN (187 aa), fasta scores opt: 446 z-score: 495.3 E(): 3.1e-20 49.7% identity in 149 aa overlap. Also similar to several hypothetical proteins e.g. Mycobacterium tuberculosis TR:O07425 (EMBL; Z97050) hypothetical 26.3 KD protein (244 aa), fasta scores opt: 269 z-score: 301.6 E(): 1.9e-09 32.7% identity in 217 aa overlap. 2 4 5 8 [Reporter]SCO0928 (14J13)_PCR/6646 [Gene]SCO0928/6054 NC_003888 SCM10.16c, hypothetical protein, len: 714 aa; similar to C-terminal region of TR:Q55576 (EMBL:D63999) Synechocystis sp. hypothetical 141.7 kD protein, 1244 aa; fasta scores: opt: 1179 z-score: 1354.7 E(): 0; 37.6% identity in 574 aa overlap and to S. coelicolor SC8D9.07 putative phosphodiesterase, 746 aa; fasta scores: opt: 817 z-score: 782.4 E(): 0; 41.8% identity in 452 aa overlap. Contains Pfam matches to entries PF00989 PAS, PAS domain, PF00785 PAC, PAC motif, PF00990 DUF9, Domain of unknown function and PF00563 DUF2, Domain of unknown function 2 2 4 5 7 [Reporter]SCO0494 (15F13)_PCR/6645 [Gene]SCO0494/6053 NC_003888 SCF34.13c, probable iron-siderophore binding lipoprotein, len: 350 aa; similar to TR:Q46023 (EMBL:U02617) Corynebacterium diphtheriae DtxR/iron-regulated lipoprotein precursor (355 aa), fasta scores; opt: 479 z-score: 518.8 E(): 1.5e-21, 34.4% identity in 358 aa overlap and SW:FATB_VIBAN (EMBL:J03529), FatB, Vibrio anguillarum plasmid pJM1 ferric anguibactin-binding protein precursor (322 aa) (26.2% identity in 305 aa overlap). Also similar to other iron-siderophore binding proteins. Contains an appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. Contains Pfam match to entry PF01497 Peripla_BP_2. Contains a probable coiled-coil from 189 to 219 (31 residues) (Max score: 1.558, probability 0.98) 2 4 5 6 [Reporter]SCO3135 (16B13)_PCR/6644 [Gene]SCO3135/6052 NC_003888 SCE66.14, probable acetyltransferase, len: 151 aa; similar to many especially SW:P19997 (BLS_STRSJ) blasticidin S-acetyltransferase (confers resistance to blasticidin S antibiotic) from Streptoverticillium sp. (strain JCM4673) (136 aa) fasta scores; opt: 465, z-score: 599.3, E(): 6.6e-26, 57.3% identity in 131 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 2 4 5 5 [Reporter]SCO3304 (17N9)_PCR/6643 [Gene]SCO3304/6051 NC_003888 SCE68.02, argS, probable arginyl-tRNA synthetase, len: 586 aa; similar to many e.g. SW:SYR_ECOLI (EMBL:X15320), argS, Escherichia coli arginyl-tRNA synthetase (577 aa), fasta scores; opt: 1415 z-score: 1562.9 E(): 0, 43.8% identity in 587 aa overlap. Contains Pfam match to entry PF00750 tRNA-synt_1d, tRNA synthetases class I (R) and PS00178 Aminoacyl-transfer RNA synthetases class-I signature 2 4 5 4 [Reporter]SCO0545 (18J9)_PCR/6642 [Gene]SCO0545/6050 NC_003888 SCF11.25, possible secreted protein, len: 843 aa; similar to TR:O87849 (EMBL:AL031013) Streptomyces coelicolor putative secreted protein, 602 aa; fasta scores: opt: 2859 z-score: 3026.4 E(): 0; 72.1% identity in 555 aa overlap. Contains possible N-terminal signal sequence 3 2 9 17 [Reporter]SCO6669 (6O3)_PCR/3216 [Gene]SCO6669/2929 NC_003888 SC5A7.19c, probable transcriptional regulator, len: 264 aa; similar to members of the iclR family e.g. GYLR_STRCO glycerol operon regulatory protein (254 aa), fasta scores; opt: 277 z-score: 318.0 E(): 1.8e-10, 31.4% identity in 245 aa overlap. Contains probable helix-turn-helix motif from aa 28-49 (Score 1240, +3.41 SD) 3 2 9 16 [Reporter]SCO3612 (7K3)_PCR/3215 [Gene]SCO3612/2928 NC_003888 SC66T3.23c, possible membrane protein, len: 454 aa; unknown function, the N-terminal third of the protein is hydrophilic, followed by a hydrophobic, possible membrane-spanning region. The N-terminus also contains 7x slightly degenerate HD(P/A)G(S/P/A)(D/G)ARDG repeats 3 2 9 15 [Reporter]SCO2162 (8G3)_PCR/3214 [Gene]SCO2162/2927 NC_003888 SC6G10A.01, probable quinolinate synthetase (fragment), len: >279 aa; similar to C-terminal part of SW:NADA_ECOLI (EMBL:X12713) Escherichia coli quinolinate synthetase A NadA, 347 aa; fasta scores: opt: 216 z-score: 269.3 E(): 1.5e-07; 26.5% identity in 283 aa overlap. Contains Pfam match to entry PF02445 NadA,SC6G10.35, possible quinolinate synthetase (fragment), len: >154 aa; similar to many e.g. TR:O32063 (EMBL:Z99118) putative NadA, quinolinate synthetase from Bacillus subtilis (368 aa) fasta scores; opt: 442, z-score: 533.6, E(): 2e-22, (60.7% identity in 107 aa overlap) and SW:NADA_ECOLI NadA, quinolinate synthetase from Escherichia coli (347 aa) fasta scores; opt: 165, z-score: 204.7, E(): 0.00043, (37.0% identity in 100 aa overlap). 3 2 9 14 [Reporter]SCO5671 (9C3)_PCR/3213 [Gene]SCO5671/2926 NC_003888 SC8B7.07c, possible oxidoreductase, len: 475 aa; some similarity to e.g. ORDL_ECOLI probable oxidoreductase ord L (426 aa), fasta scores; opt: 413 z-score: 280.3 E(): 2.4 e-08, 32.1% identity in 436 aa overlap 3 2 9 13 [Reporter]SCO1511 (9O23)_PCR/3212 [Gene]SCO1511/2925 NC_003888 SCL2.01, hypothetical protein (fragment), len: >328 aa; similar to TR:O33236 (EMBL:Z98209) Mycobacterium tuberculosis hypothetical 49.8 kD protein MTCY174.11, len: 450 aa; fasta scores: opt: 843 z-score: 874.7 E(): 0; 45.9% identity in 296 aa overlap. Contains possible coiled-coils region,SC9C5.35, hypothetical protein (fragment), len: >57 aa 3 2 9 12 [Reporter]SCO2682 (10K23)_PCR/3211 [Gene]SCO2682/2924 NC_003888 SCC61A.03c, possible membrane protein, len: 470 aa; contains possible membrane-spanning hydrophobic regions. 2 4 5 3 [Reporter]SCP1.206c (19F9)_PCR/6641 [Gene]SCP1.206c/6049 NC_003888 SCP1.206c, unknown, len: 175aa; 3 2 9 11 [Reporter]SCO4678 (11G23)_PCR/3210 [Gene]SCO4678/2923 NC_003888 SCD31.03, conserved hypothetical protein, len: 282 aa; similar to various hypothetical proteins, e.g. TR:CAB66187 (EMBL:AL136500) Streptomyces coelicolor hypothetical 31.7 kD protein SC1G2.15c, 284 aa; fasta scores: opt: 1359 z-score: 1611.1 E(): 0; 71.7% identity in 279 aa overlap 2 4 5 2 [Reporter]SCP1.250 (20N5)_PCR/6640 [Gene]SCP1.250/6048 NC_003888 SCP1.250, unknown, len: 140aa; 3 2 9 10 [Reporter]SCO3392 (12C23)_PCR/3209 [Gene]SCO3392/2922 NC_003888 SCE126.10, nuoD2, probable NADH dehydrogenase subunit, len: 383 aa; similar to many others e.g. SW:NUOD_ECOLI NADH dehydrogenase chain D (407 aa) fasta scores; opt: 689, z-score: 821.3, E(): 0, (33.2% identity in 397 aa overlap). Contains two Pfam matches to entry PF00346 complex1_49Kd, Respiratory-chain NADH dehydrogenase, 49 Kd subunit and Prosite match to PS00535 Respiratory chain NADH dehydrogenase 49 Kd subunit signature. 2 4 4 22 [Reporter]SCO4537 (1N1)_PCR/6638 [Gene]SCO4537/6047 NC_003888 2SCD4.08, unknown, len: 201 aa 3 2 9 9 [Reporter]SCO1156 (13O19)_PCR/3208 [Gene]SCO1156/2921 NC_003888 SCG8A.10, conserved hypothetical protein, len: 142aa; similar to many eg. TR:O52559 (EMBL:AF040570) hypothetical protein from Amycolatopsis mediterranei (166 aa) fasta scores; opt: 222, z-score: 288.8, E(): 1.2e-08, 31.6% identity in 136 aa overlap. 2 4 4 21 [Reporter]SCO5367 (2J1)_PCR/6637 [Gene]SCO5367/6046 NC_003888 2SC6G5.11, atpB, ATP synthase A chain (EC 3.6.1.34), len: 273aa; similar to many eg. SW:P15012 (ATP6_RHORU) ATP synthase A chain from Rhodospirillum rubrum (241 aa) fasta scores; opt: 356, z-score: 432.3, E(): 1.3e-16, 35.7% identity in 252 aa overlap. Identical to SW:P50012 (ATP6_STRLI) ATP synthase A chain from Streptomyces lividans. Contains Pfam match to entry PF00119 ATP-synt_A, ATP synthase A chain and Prosite match to PS00449 ATP synthase a subunit signature. Contains possible membrane-spanning hydrophobic regions. 3 2 9 8 [Reporter]SCO0207 (14K19)_PCR/3207 [Gene]SCO0207/2920 NC_003888 SCJ12.19c, unknown, len: 201 aa. Weakly similar to many hypotheticals e.g. Thermus aquaticus SW:YQA3_THEAQ (EMBL; D87664) hypothetical 14.6 KD protein in QAH/OAS sulfhydrylase 3'region (137 aa), fasta scores opt: 172 z-score: 213.8 E(): 0.00015 35.9% identity in 142 aa overlap. Also similar to the N-terminal region of TR:Q9RI46 (EMBL:AL109989) Streptomyces coelicolor hypothetical protein SCJ12.12c, 301 aa; fasta scores: opt: 466 Z-score: 532.5 E(): 5e-22; 42.500% identity in 200 aa overlap 2 4 4 20 [Reporter]SCO4574 (3F1)_PCR/6636 [Gene]SCO4574/6045 NC_003888 SCD16A.09c, nuoM, NADH dehydrogenase subunit, len: 523 aa; similar to many e.g. TR:O53307 (EMBL:AL021646) NuoM, NADH dehydrogenase subunit from Mycobacterium tuberculosis (553 aa) fasta scores; opt: 1621, z-score: 1842.0, E(): 0, (56.6% identity in 541 aa overlap) and SW:NUOM_ECOLI, NADH dehydrogenase subunit NuoM from Escherichia coli (509 aa) fasta scores; opt: 970, z-score: 1103.8, E(): 0, (35.1% identity in 513 aa overlap). Contains Pfam match to entry PF00361 oxidored_q1, NADH-Ubiquinone/plastoquinone (complex I), various chains. Contains possible membrane spanning hydrophobic regions. 2 4 4 19 [Reporter]SCO6155 (4B1)_PCR/6635 [Gene]SCO6155/6044 NC_003888 SC1A9.19, poxB, pyruvate oxidase, len: 580aa; similar to many including POXB_ECOLI (EMBL:X04105) PoxB, pyruvate oxidase from Escherichia coli (572 aa) fasta scores; opt:2086, z-score: 2458.4, E():0, (53.1% identity in 571 aa overlap). Contains Pfam match to entry PF00205 TPP_enzymes, Thiamine pyrophosphate enzymes, score 499.70, E-value 2.4e-154. 2 4 4 18 [Reporter]SCO6229 (4N21)_PCR/6634 [Gene]SCO6229/6043 NC_003888 SC2H4.11c, probable sugar transport system permease protein, len: 293 aa; similar to many e.g. MALD_STRPN maltodextrin transport system permease protein (277 aa), fasta scores; opt: 394 z-score: 585.4 E(): 2.3e-25, 29.1% identity in 268 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 58.30, E-value 1.6e-13 2 4 4 17 [Reporter]SCO7049 (5J21)_PCR/6633 [Gene]SCO7049/6042 NC_003888 SC4G1.15, probable glutaminase, len: 307 aa; similar to TR:O87405 (EMBL:AF057158) Rhizobium etli glutaminase A, 309 aa; fasta scores: opt: 990 z-score: 1158.2 E(): 0; 52.3% identity in 304 aa overlap and to middle part of SW:GLSK_RAT (EMBL:M65150) Rattus norvegicus glutaminase, kidney isoform precursor (EC 3.5.1.2) GlsK, 647 aa; fasta scores: opt: 577 z-score: 672.7 E(): 5.3e-30; 35.2% identity in 293 aa overlap 2 4 4 16 [Reporter]SCO7581 (6F21)_PCR/6632 [Gene]SCO7581/6041 NC_003888 SC5F1.35c, hypothetical protein, len: 556 aa; low similarity to SW:Y811_METJA (EMBL:U67525) Methanococcus jannaschii hypothetical protein MJ0811, 439 aa; fasta scores: opt: 321 z-score: 347.7 E(): 7.7e-12 26.8% identity in 287 aa overlap 2 4 4 15 [Reporter]SCO7417 (7B21)_PCR/6631 [Gene]SCO7417/6040 NC_003888 SC6D11.13c, possible cytochrome P450-family protein, len: 407 aa. Similar to many members of the cytochrome P450 family including: Streptomyces griseus SW:CPXH_STRGR (EMBL:X63601) cytochrome P450-soy (412 aa), fasta scores opt: 673 z-score: 761.0 E():0 35.1% identity in 382 aa overlap and Saccharopolyspora erythraea (Streptomyces erythraeus) SW:CPXK_SACER (EMBL:M83110) cytochrome P450 107B1 (405 aa), fasta scores opt: 872 z-score: 984.9 E():0 38.4% identity in 409 aa overlap. Contains a Prosite hit to PS00086 Cytochrome P450 cysteine heme-iron ligand signature and a Pfam match to entry PF00067 p450, Cytochrome P450. 3 2 9 7 [Reporter]SCO1517 (15G19)_PCR/3206 [Gene]SCO1517/2919 NC_003888 SCL2.07c, possible secreted protein, len: 169 aa; similar to SW:YP88_MYCLE (EMBL:AL023591) Mycobacterium leprae hypothetical protein MCLB1259.04, 114 aa; fasta scores: opt: 205 z-score: 231.1 E(): 1.8e-05; 36.7% identity in 120 aa overlap. Contains possible N-terminal region signal peptide sequence 3 2 9 5 [Reporter]SCO1452 (17O15)_PCR/3204 [Gene]SCO1452/2918 NC_003888 SCL6.09, unknown, len: 231 aa 3 2 9 4 [Reporter]SCO1291 (18K15)_PCR/3203 [Gene]SCO1291/2917 NC_003888 SCBAC36F5.02, unknown, len: 467 aa 3 2 9 3 [Reporter]SCP1.231 (19G15)_PCR/3202 [Gene]SCP1.231/2916 NC_003888 SCP1.231, mmyQ, hypothetical protein, len: 217aa; previously sequenced and annotated as TR:Q9JN78 (EMBL:AJ276673). Similar to TR:Q9L1E0 (EMBL:AL157916) hypothetical protein from Streptomyces coelicolor (220 aa) fasta scores; opt: 314, z-score: 377.8, E(): 1.4e-13, 35.2% identity in 199 aa overlap. 3 2 9 2 [Reporter]SCO5078 (20O11)_PCR/3201 [Gene]SCO5078/2915 NC_003888 SCBAC28G1.04, actVA3, hypothetical protein, len: 281 aa; identical to previously sequenced TR:Q53905 (EMBL:X58833) Streptomyces coelicolor 6 ActVA region genes of the actinorhodin biosynthetic gene cluster, ActVA3, 281 aa 3 2 8 22 [Reporter]SCO4227 (1O7)_PCR/3199 [Gene]SCO4227/2914 NC_003888 2SCD46.41c, unknown, len: 421aa; 2 4 4 14 [Reporter]SCO2019 (8N17)_PCR/6630 [Gene]SCO2019/6039 NC_003888 SC7H2.33c, possible chorismate mutase, len: 116aa; similar to the N-terminal region (which has been shown to contain chorismate mutase activity) of SW:TYRA_ERWHE T-protein chorismate mutase/chorismate dehydrogenase bifunctional protein from Erwinia herbicola (373 aa) fasta scores; opt: 179, z-score: 228.0, E(): 2.2e-05, (38.5% identity in 78 aa overlap). 3 2 8 21 [Reporter]SCO1848 (2K7)_PCR/3198 [Gene]SCO1848/2913 NC_003888 SCI8.33, cobQ, cobyric acid synthase, len: 502 aa. Highly similar to many cobyric acid synthases e.g. Salmonella typhimurium SW:CBIP_SALTY (EMBL:L12006) CbiP (506 aa), fasta scores opt: 814 z-score: 928.5 E():0 44.2% identity in 511 aa overlap and Pseudomonas denitrificans SW:COBQ_PSEDE (EMBL:M62866) CobQ (484 aa), fasta scores opt: 1066 z-score: 1215.9 E(): 0 42.3% identity in 494 aa overlap. Contains a Pfam match to entry PF01656 CBIA, Cobyrinic acid A,C-diamide synthase and a possible membrane spanning hydrophobic domain. 2 4 4 13 [Reporter]SCO6130 (9J17)_PCR/6629 [Gene]SCO6130/6038 NC_003888 SC9B2.17, unknown, len: 117 aa; similar to many proteins of undefined function some of which are proposed to be regulatory proteins (by similarity) eg. TR:Q53897 (EMBL:X60316) ORFA of the AbaA locus from Streptomyces coelicolor (192 aa) fasta scores; opt: 255, z-score: 325.2, E(): 7.9e-11, (40.9% identity in 110 aa overlap) and TR:E1359165 (EMBL:AL034446) putative regulatory protein from Streptomyces coelicolor (135 aa) fasta scores; opt: 234, z-score: 301.8, E(): 1.6e-09, (43.0% identity in 121 aa overlap). 3 2 8 19 [Reporter]SCO6049 (4C7)_PCR/3196 [Gene]SCO6049/2912 NC_003888 SC1B5.09c, unknown, len: 76 aa 2 4 4 12 [Reporter]SCO2451 (10F17)_PCR/6628 [Gene]SCO2451/6037 NC_003888 SCC24.22, probable rod shape-determining protein, len: 360 aa; similar to SW:MREB_BACSU (EMBL:M95582) Bacillus subtilis rod-shape determining protein MreB, 337 aa; fasta scores: opt: 740 z-score: 810.4 E(): 0; 34.8% identity in 322 aa overlap and to TR:CAB75392 (EMBL:AL139298) Streptomyces coelicolor rod shape-determining protein MreB, 343 aa; fasta scores: opt: 868 z-score: 835.5 E(): 0; 43.1% identity in 320 aa overlap 3 2 8 18 [Reporter]SCO5892 (5O3)_PCR/3195 [Gene]SCO5892/2911 NC_003888 SC3F7.12, redL, probable polyketide synthase, len: 2297 aa; contains 3 modules: N-terminus (start to approx. aa 700) similar to eg. N-terminus of TR:Q50857 (EMBL:U24657) saframycin mx1 synthetase B from Myxococcus xanthus (1770 aa) (fasta scores; E(): 0, 45.0% identity in 691 aa overlap), contains Pfam match to AMP-binding PF00501, AMP-binding enzymes, score 166.66; central module (approx aa 795 to 1800) similar to eg. TR:G2317861 (EMBL:U78289) Streptomyces fradiae tylactone synthase module 3 (1864 aa) (E(): 0, 39.9% identity in 1125 aa overlap), contains PS00606 Beta-ketoacyl synthases active site and Pfam match to ketoacyl-synt PF00109, Beta-ketoacyl synthases, score 585.24; and C-terminus (approx aa 1800 to end), similar to eg. TR:Q52936 (EMBL:X64131 acyl-transferase rkpG from Rhizobium meliloti (446 aa) (E(): 0. 44.9% identity in 394 aa overlap), contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site and Pfam match to aminotran_2 PF00222, Aminotransferases class-II, score 276.93 2 4 4 11 [Reporter]SCO4355 (11B17)_PCR/6627 [Gene]SCO4355/6036 NC_003888 SCD19.10, probable nucleotidyltransferase, len: 257 aa; similar to SW:S3AD_STAAU (EMBL:X02588) Staphylococcus aureus streptomycin 3''-adenylyltransferase (EC 2.7.7.47) Spc, 260 aa; fasta scores: opt: 644 z-score: 760.8 E(): 0; 44.5% identity in 227 aa overlap. Contains Pfam match to entry PF01909 NTP_transf_2, Nucleotidyltransferase domain 3 2 8 17 [Reporter]SCO6665 (6K3)_PCR/3194 [Gene]SCO6665/2910 NC_003888 SC5A7.15c, probable secreted glucosidase, len: 466 aa; similar to e.g. TR:G2981143 (EMBL:AF052745) beta-1,3-glucanase II from Oerskovia xanthineolytica (435 aa), fasta scores; opt: 313 z-score: 413.3 E(): 8.7e-16, 37.0% identity in 435 aa overlapand XYND_BACPO endo-1,4-beta-xylanase D precursor (Bacillus polymyxa) (635 aa), fasta scores; opt: 339 z-score: 315.2 E(): 2.6e-10, 27.5% identity in 447 aa overlap 2 4 4 10 [Reporter]SCO3517 (12N13)_PCR/6626 [Gene]SCO3517/6035 NC_003888 SCE134.18, possible ATP-binding protein, len: 489aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). Similar to TR:Q9L1N7 (EMBL:AL138977) from Streptomyces coelicolor (507 aa) fasta scores; opt: 2405, Z-score: 2578.8, 73.469% identity (75.000% ungapped) in 490 aa overlap. 2 4 4 9 [Reporter]SCO1166 (13J13)_PCR/6625 [Gene]SCO1166/6034 NC_003888 SCG8A.20c, possible integral membrane protein, len: 110aa; Contains possible membrane-spanning hydrophobic region. 2 4 4 8 [Reporter]SCO1159 (14F13)_PCR/6624 [Gene]SCO1159/6033 NC_003888 SCG8A.13c, cvnB3, hypothetical protein, len: 145 aa; strongly similar to many from Streptomyces coelicolor e.g. TR:Q9X835 (EMBL:AL049727) hypothetical 14.4 kD protein (139 aa) fasta scores; opt: 412, z-score: 512.5, E(): 4.2e-21, 52.8% identity in 127 aa overlap. Also strongly similar to neighbouring CDS SCG8A.09c. Contains Pfam match to entry PF01860 DUF42, Protein of unknown function 2 4 4 7 [Reporter]SCO3223 (15B13)_PCR/6623 [Gene]SCO3223/6032 NC_003888 SCE8.16c, probable ABC transporter integral membrane protein, len: 266aa; located directly downstream of a probable ABC transporter ATP-binding protein (6bp gap). Similar to many particularly TR:O86630 (EMBL:AL031155) an integral membrane protein located directly downstream of a probable ABC transporter ATP-binding protein (2bp gap) from Streptomyces coelicolor (279 aa) fasta scores; opt: 460, z-score: 539.7, E(): 9.4e-23, (37.0% identity in 273 aa overlap). Contains several probable membrane spanning hydrophobic regions. 2 4 4 6 [Reporter]SCO1789 (16N9)_PCR/6622 [Gene]SCO1789/6031 NC_003888 SCI51.29, hypothetical protein, len: 430 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 2 4 4 5 [Reporter]SCO3918 (17J9)_PCR/6621 [Gene]SCO3918/6030 NC_003888 SCQ11.01c, hypothetical protein, partial CDS, len: >95 aa; unknown function, previously sequenced as TR:Q53870 (EMBL:U37580), S.coelicolor hypothetical protein ORF5 downstream of phosphotyrosine protein phosphatase (159 aa). Weak similarity to the N-terminus of TR:O69888 (EMBL:AL023797) Streptomyces coelicolor hypothetical protein (172 aa), fasta scores; opt: 158 z-score: 206.4 E(): 0.00036, 46.2% identity in 65 aa overlap. Continues as SCH24.40c in cosmid H24 (EMBL:AL049826),SCH24.40c, hypothetical protein, partial CDS, len: >96 aa; unknown function, highly similar to TR:Q53870 (EMBL:U37580) S.coelicolor hypothetical protein ORF5 downstream of phosphotyrosine protein phosphatase (159 aa), fasta scores; opt: 615 z-score: 859.5 E(): 0, 94.8% identity in 96 aa overlap 3 2 8 16 [Reporter]SCO0316 (7G3)_PCR/3193 [Gene]SCO0316/2909 NC_003888 SC5G9.25c, hypothetical protein, len: 555 aa; similar to hypothetical proteins e.g. TR:O50442 (EMBL:AL010186) Mycobacterium tuberculosis hypothetical protein (538 aa), fasta scores; opt: 485 z-score: 521.2 E(): 1.2e-21, 32.6% identity in 568 aa overlap and to TR:Q9Z561 (EMBL:AL035569) S.coelicolor putative transcriptional regulator (415 aa) (28.4% identity in 444 aa overlap). Contains probable helix-turn-helix motif at aa 505-526 (Score 1081, +2.87 SD) 3 2 8 15 [Reporter]SCO2158 (8C3)_PCR/3192 [Gene]SCO2158/2908 NC_003888 SC6G10.31c, probable kinase, len: 338 aa; similar to many e.g. SW:RBSK_BACSU ribokinase from Bacillus subtilis (293 aa) fasta scores; opt: 222, z-score: 264.5, E(): 2e-07, (26.6% identity in 252 aa overlap). Contains Prosite matches to PS00583 pfkB family of carbohydrate kinases signature 1 and PS00485 Adenosine and AMP deaminase signature. 3 2 8 14 [Reporter]SCO6582 (8O23)_PCR/3191 [Gene]SCO6582/2907 NC_003888 SC8A6.03c, unknown, len: 714 aa; similar to many hypothetical proteins including several from each of the arche ons Archaeoglobus fulgidus TR:O28341 (EMBL:AE000970) conser ved hypothetical protein AF1938(673 aa), fasta scores; opt: 960 z-score: 1289.8 E(): 0, 35.6% identity in 693 aa overl ap and Pyrococcus horikoshii D1028624 (EMBL:AB009518) hypot hetical protein PHCV016 (460 aa), fasta scores; opt: 1280 z -score: 1246.9 E(): 0, 44.3% identity in 461 aa overlap. Co ntains PS00216 Sugar transport proteins signature 1. Also s imilar to S. coelicolor hypothetical protein SC9B10.09 (E() : 6.1e-15, 33.9% identity in 375 aa overlap) 3 2 8 13 [Reporter]SCO1506 (9K23)_PCR/3190 [Gene]SCO1506/2906 NC_003888 SC9C5.30c, conserved ATP/GTP binding protein, len: 451 aa; similar to SW:Y0A9_MYCTU (EMBL:Z77250) Mycobacterium tuberculosis hypothetical 47.5 kD protein MTCY9C4.09, 452 aa; fasta scores: opt: 1742 z-score: 1796.7 E(): 0; 64.4% identity in 430 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 2 8 12 [Reporter]SCO2727 (10G23)_PCR/3189 [Gene]SCO2727/2905 NC_003888 SCC46.12c, improbable CDS predicted by HMM, len: 125 aa 3 2 8 11 [Reporter]SCO4096 (11C23)_PCR/3188 [Gene]SCO4096/2904 NC_003888 SCD25.32, ATP-dependent RNA helicase, 839 aa. Highly similar to many including: Streptomyces coelicolor TR:O54116 (EMBL; AL021529) DEAD-box RNA helicase SC10A5.25C (498 aa), fasta scores opt: 1467 z-score: 1347.1 E(): 0 50.0% identity in 486 aa overlap. Contains Pfam matches to entry PF00270 DEAD, DEAD/DEAH box helicase and Pfam match to entry PF00271 helicase_C, Helicases conserved C-terminal domain, as well as a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). Also contains a degenerate direct repeat region: containing several different repeats: 6.5x RRDDRGDRGGRSF(D/E) 2x RRDDRRDDRGGRSFE 2x RRDDRGGF and a probable coiled-coil from 444 to 501 (58 residues). 2 4 4 4 [Reporter]SCO1590 (18F9)_PCR/6620 [Gene]SCO1590/6029 NC_003888 SCI35.12c, putative secreted protein, len: 414 aa; similar to hypothetical proteins from several organisms e.g. E. coli TR:P76130 (EMBL:AE000246) (439 aa), fasta scores; opt: 879 z-score: 1103.6 E(): 0, 39.6% identity in 407 aa overlap. Contains possible N-terminal region signal peptide sequence 3 2 8 10 [Reporter]SCO2932 (12O19)_PCR/3187 [Gene]SCO2932/2903 NC_003888 SCE19A.32, probable permease membrane component, len: 290 aa; similar to many, including components of transporters involved in osmoregulation e.g. SW:PROW_BACSU (EMBL:U38418), proW, Bacillus subtilis glycine betaine/L-proline transport system permease protein (217 aa), fasta scores; opt: 429 z-score: 461.7 E(): 2.2e-18, 37.7% identity in 220 aa overlap. Similar to SCE19A.30, probable permease inner membrane component (215 aa) (33.5% identity in 209 aa overlap) and SC9B1.11 (EMBL:AL049727) probable ABC transport permease (221 aa) (37.6% identity in 186 aa overlap). Contains an approx. 50 aa N-terminal extension absent from similar proteins and hydrophobic, possible membrane-spanning regions. May be part of a multicomponent transporter which includes SCE19A.30, SCE19A.31, SCE19A.32 and SCE19A.33. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 2 4 4 3 [Reporter]SCP1.89c (19B9)_PCR/6619 [Gene]SCP1.89c/6028 NC_003888 SCP1.89c, unknown, len: 389aa; 3 2 8 9 [Reporter]SCO3365 (13K19)_PCR/3186 [Gene]SCO3365/2902 NC_003888 SCE94.16c, unknown, len: aa; similar to others from Streptomyces coelicolor eg. TR:E1370777 (EMBL:AL035212) possible DNA binding protein (303 aa) fasta scores; opt: 420, z-score: 512.3, E(): 3.2e-21, (32.5% identity in 265 aa overlap). 2 4 4 2 [Reporter]SCO1362 (20J5)_PCR/6618 [Gene]SCO1362/6027 NC_003888 SC10A9.04c, possible integral membrane protein, len: 324 aa; similar to SW:YWFM_BACSU (EMBL:X73124) Bacillus subtilis hypothetical 31.3 kDa protein in Pta 3' region Ywfm, 296 aa; fasta scores: opt: 267 z-score: 282.4 E(): 2.7e-08; 26.9% identity in 305 aa overlap. Contains 2x Pfam matches to entry PF00892 DUF6, Integral membrane protein DUF6. Also contains possible hydrophobic membrane spanning regions and a 4x degenerate repeat: LRP(R/A) 3 2 8 8 [Reporter]SCO0703 (14G19)_PCR/3185 [Gene]SCO0703/2901 NC_003888 SCF42.13, possible regulator, len: 75 aa. Identical to the previously published Streptomyces coelicolor TR:Q53896 (EMBL:X60316) pleiotropic antibiotic regulatory locus AbaA, OrfD (75 aa), fasta scores opt: 507 z-score: 699.1 E(): 1.5e-31 98.7% identity in 75 aa overlap. Also highly similar to Streptomyces coelicolor TR:Q9X8K3 (EMBL:AL049841) putative regulator SCE9.31c (83 aa), fasta scores opt: 308 z-score: 431.8 E(): 1.2e-16 63.6% identity in 77 aa overlap. 2 4 3 22 [Reporter]SCO4533 (1J1)_PCR/6616 [Gene]SCO4533/6026 NC_003888 2SCD4.04, hypothetical protein, len: 108 aa; similar to TR:CAB77307 (EMBL:AL160312) Streptomyces coelicolor hypothetical 13.8 kDa protein SCD35.23c, 119 aa; fasta scores: opt: 290 z-score: 364.1 E(): 8.2e-13; 47.6% identity in 105 aa overlap 3 2 8 7 [Reporter]SCO4965 (15C19)_PCR/3184 [Gene]SCO4965/2900 NC_003888 2SCK31.25c, probable transcription elongation factor, len: 165 aa; similar to SW:GREA_ECOLI (EMBL:X54718) Escherichia coli transcription elongation factor GreA (transcript cleavage factor), 158 aa; fasta scores: opt: 265 z-score: 326.5 E(): 1.2e-10; 34.0% identity in 153 aa overlap. Contains Pfam match to entry PF01272 GreA_GreB, Prokaryotic transcription elongation factor, GreA/GreB and match to Prosite entry PS00830 Prokaryotic transcription elongation factors signature 2 2 4 3 21 [Reporter]SCO0725 (2F1)_PCR/6615 [Gene]SCO0725/6025 NC_003888 3SC5B7.03, unknown, len: 125 aa 2 4 3 20 [Reporter]SCO4572 (3B1)_PCR/6614 [Gene]SCO4572/6024 NC_003888 SCD16A.11c, nuoK, NADH dehydrogenase subunit, len: 99 aa; similar to many e.g. SW:NUOK_MYCTU NuoK, NADH dehydrogenase subunit from Mycobacterium tuberculosis (99 aa) fasta scores; opt: 503, z-score: 724.2, E(): 5.2e-33, (77.6% identity in 98 aa overlap) and SW:NUOK_ECOLI, NADH dehydrogenase subunit NuoK from Escherichia coli (100 aa) fasta scores; opt: 253, z-score: 373.2, E(): 1.8e-13, (40.2% identity in 97 aa overlap). Contains Pfam match to entry PF00420 oxidored_q2, NADH-ubiquinone/plastoquinone oxidoreductase chain 4L. Contains possible membrane spanning hydrophobic regions. 2 4 3 19 [Reporter]SCO1396 (3N21)_PCR/6613 [Gene]SCO1396/6023 NC_003888 SC1A8A.16c, probable D-alanyl-D-alanine dipeptidase (putative secreted protein), len: 274aa; similar to many eg. TR:Q9X523 (EMBL:AF120672) pcgL, D-alanyl-D-alanine dipeptidase from Salmonella typhimurium (256 aa) fasta scores; opt: 851, z-score: 983.2, E(): 0, 49.6% identity in 252 aa overlap. Contains two Pfam matches to entry PF01427 Peptidase_M45, D-ala-D-ala dipeptidase and possible N-terminal region signal peptide sequence 2 4 3 18 [Reporter]SCO6225 (4J21)_PCR/6612 [Gene]SCO6225/6022 NC_003888 SC2H4.07c, probable secreted protein, len: 421 aa; similar in C-terminus to B. subtilis TR:P70960 (EMBL:Z81356;) hypothetical protein ywmC precursor (227 aa), fasta scores; opt: 319 z-score: 219.2 E(): 5.8e-05, 29.6% identity in 216 aa overlap 2 4 3 17 [Reporter]SCO7121 (5F21)_PCR/6611 [Gene]SCO7121/6021 NC_003888 SC4B10.22, possible secreted protein, len: 281 aa; similar to TR:Q9S1Z1 (EMBL:AL109747) Streptomyces coelicolor hypothetical 24.6 kDa protein SCJ21.16, 239 aa; fasta scores: opt: 989 z-score: 1075.6 E(): 0; 64.4% identity in 233 aa overlap and to Streptomyces coelicolor SC4B10.33, 239 aa; fasta scores: opt: 1034 z-score: 972.0 E(): 0; 67.0% identity in 233 aa overlap. Contains possible N-terminal region signal peptide sequence 2 4 3 16 [Reporter]SCO7577 (6B21)_PCR/6610 [Gene]SCO7577/6020 NC_003888 SC5F1.31, possible secreted hydrolase, len: 369 aa; similar to TR:P82594 (EMBL:AB023626) Streptomyces chartreusis alpha-L-arabinofuranosidase II precursor (EC 3.2.1.55), 328 aa; fasta scores: opt: 336 z-score: 382.3 E(): 9.2e-14; 27.8% identity in 302 aa overlap. Contains possible N-terminal region signal peptide sequence 2 4 3 15 [Reporter]SCO2124 (7N17)_PCR/6609 [Gene]SCO2124/6019 NC_003888 SC6E10.18, putative membrane protein, len: 205 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. The amino acid composition of this CDS is unusual, being Pro-rich and having a very acidic N-terminus. Contains probable coiled-coil from 1 to 29 (29 residues) (Max score: 1.455, probability 0.88). Contains possible hydrophobic membrane spanning regions 2 4 3 14 [Reporter]SCO2015 (8J17)_PCR/6608 [Gene]SCO2015/6018 NC_003888 SC7H2.29, possible secreted nucleotidase, len: 602 aa; similar to many e.g. SW:CN16_ECOLI periplasmic 2',3'-cyclic phosphodiesterase from Escherichia coli (647 aa) fasta scores; opt: 577, z-score: 618.9, E(): 3.8e-27, (32.3% identity in 595 aa overlap). Contains Pfam match to entry PF01009 5_nucleotidase, 5'-nucleotidase and Prosite match to PS00786 5'-nucleotidase signature 2. Also has possible N-terminal signal sequence. 2 4 3 13 [Reporter]SCO5743 (9F17)_PCR/6607 [Gene]SCO5743/6017 NC_003888 SC9A10.07, unknown, len: 246 aa; similar to e.g. Dictyostelium discoideum THY1_DICDI THY1 protein (260 aa), fasta scores; opt: 308 z-score: 443.0 E(): 2.1e-17, 35.4% identity in 198 aa overlap 2 4 3 12 [Reporter]SCO2447 (10B17)_PCR/6606 [Gene]SCO2447/6016 NC_003888 SCC24.18, unknown, len: 153 aa 2 4 3 11 [Reporter]SCO4699 (11N13)_PCR/6605 [Gene]SCO4699/6015 NC_003888 SCD31.24, possible Rhs protein, len: 927 aa; similar to C-terminal domain of SW:RHSB_ECOLI (EMBL:L02370) Escherichia coli RhsB protein precursor, 1411 aa; fasta scores: opt: 510 z-score: 568.8 E(): 3e-24; 26.4% identity in 954 aa overlap 2 4 3 10 [Reporter]SCO3513 (12J13)_PCR/6604 [Gene]SCO3513/6014 NC_003888 SCE134.14, possible secreted protein, len: 226 aa. Contains possible N-terminal region signal peptide sequence 2 4 3 9 [Reporter]SCO3920 (13F13)_PCR/6603 [Gene]SCO3920/6013 NC_003888 SCQ11.03c, cysA, probable cystathionine/methionine gamma-synthase/lyase, len: 392 aa; previously sequenced as SW:CYSA_STRCO (EMBL:U37580), cysA, S.coelicolor putative cystathionine gamma-lyase (392 aa). Similar to many e.g. SW:MEGL_PSEPU (EMBL:D88554), mdeA, Pseudomonas putida methionine gamma-lyase (398 aa), fasta scores; opt: 608 z-score: 653.6 E(): 4.5e-29, 36.9% identity in 404 aa overlap. Highly similar to TR:Q53668 (EMBL:X91393) Streptomyces antibioticus hypothetical protein found upstream of the abaB gene (232 aa) (87.1% identity in 232 aa overlap). Contains Pfam match to PF01053 Cys_Met_Meta_PP, Cys/Met metabolism PLP-dependent enzyme and PS00868 Cys/Met metabolism enzymes pyridoxal-phosphate attachment site 2 4 3 8 [Reporter]SCO1825 (14B13)_PCR/6602 [Gene]SCO1825/6012 NC_003888 SCI8.10, possible membrane protein, len: 212 aa. Contains a possible membrane spanning hydrophobic domain. 2 4 3 7 [Reporter]SCO3927 (15N9)_PCR/6601 [Gene]SCO3927/6011 NC_003888 SCQ11.10c, possible membrane protein, len: 513 aa; unknown function, probable CDS with Pro/Arg-rich N-terminus and 4x degenerate GGGHGA repeats from aa 58-81. Contains hydrophobic, possible membrane-spanning regions 2 4 3 6 [Reporter]SCO0890 (16J9)_PCR/6600 [Gene]SCO0890/6010 NC_003888 SCM1.23c, possible short chain oxidoreductase, len: 235 aa. Similar to several including: Bradyrhizobium japonicum SW:FIXR_BRAJA(EMBL:X06167) FixR protein involved in symbiotic nitrogen fixation (278 aa), fasta scores opt: 262 z-score: 283.4 E(): 1.9e-08 30.8% identity in 224 aa overlap and Streptomyces coelicolor TR:CAB56136(EMBL:AL117669) putative short chain oxidoreductase (299 aa), fasta scores opt: 290 z-score: 311.9 E(): 5e-10 38.6% identity in 233 aa overlap. Contains a Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 2 4 3 5 [Reporter]SCO0369 (17F9)_PCR/6599 [Gene]SCO0369/6009 NC_003888 SCF41.28c, putative secreted protein, len: 1085 aa. Contains a possible N-terminal signal peptide sequence 2 4 3 3 [Reporter]SCO4891 (19N5)_PCR/6597 [Gene]SCO4891/6008 NC_003888 2SCK8.17c, putative secreted protein, len: 128 aa. Contains possible N-terminal region signal peptide sequence 2 4 2 22 [Reporter]SCO5182 (1F1)_PCR/6594 [Gene]SCO5182/6007 NC_003888 2SC3B6.06, conserved hypothetical protein, len: 183 aa; similar to TR:Q9RX87 (EMBL:AE001902) Deinococcus radiodurans conserved hypothetical protein DR0428, 139 aa; fasta scores: opt: 187 z-score: 230.6 E(): 2.2e-05; 35.2% identity in 122 aa overlap 2 4 2 21 [Reporter]SCO4914 (2B1)_PCR/6593 [Gene]SCO4914/6006 NC_003888 SCK13.06c, probable deoxyribose-phosphate aldolase, len: 331 aa; similar to SW:DEOC_ECOLI (EMBL:X03224) Escherichia coli deoxyribose-phosphate aldolase (EC 4.1.2.4) DeoC, 259 aa; fasta scores: opt: 663 z-score: 743.7 E(): 0; 47.3% identity in 256 aa overlap. Contains Pfam match to entry PF01791 DeoC, Deoxyribose-phosphate aldolase 2 4 2 20 [Reporter]SCO0217 (2N21)_PCR/6592 [Gene]SCO0217/6005 NC_003888 SCJ12.29, narH2, probable nitrate reductase beta chain, len: 522 aa. Highly similar to many e.g. Streptomyces coelicolor TR:O86716 (EMBL; AL031515) putative nitrate reductase beta chain NarH SC5C7.19c (531 aa), fasta scores: opt: 2348 z-score: 2413.0 E(): opt: 2348 z-score: 2413.0 E(): 0 65.3% identity in 496 aa overlap and Bacillus subtilis SW:NARH_BACSU (EMBL; Z49884) nitrate reductase beta chain (EC 1.7.99.4) NarH (487 aa), fasta scores: opt: 2081 z-score: 2139.7 E(): 0 57.1% identity in 485 aa overlap. Contains a PS00190 Cytochrome c family heme-binding site signature, a Pfam match to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains and a Pfam match to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains. 2 4 2 19 [Reporter]SCO1391 (3J21)_PCR/6591 [Gene]SCO1391/6004 NC_003888 SC1A8A.11, phosphoenolpyruvate-protein phosphotransferase, len: 556 aa; similar to many e.g. SW:P08839 (PT1_ECOLI) PtsI, phosphoenolpyruvate-protein phosphotransferase from Escherichia coli (575 aa) fasta scores; opt: 473, z-score: 494.3, E(): 4.2e-20, 34.6% identity in 570 aa overlap. Contains Pfam match to entry PF00391 PEP-utilizers, PEP-utilizing enzymes, Prosite match to PS00370 PEP-utilizing enzymes phosphorylation site signature, Prosite match to PS00742 PEP-utilizing enzymes signature 2 and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 2 4 2 18 [Reporter]SCO5875 (4F21)_PCR/6590 [Gene]SCO5875/6003 NC_003888 SC2E9.16c, possible potassium uptake protein; len: 226 aa; similar to many homologs of trkA eg. Mycobacterium tuberculosis TR:O07194 (EMBL:Z96072) MTCY05A6.13 (220 aa), fasta scores; opt: 870 z-score: 1486.1 E(): 0, 58.8% identity in 216 aa overlap. Also similar to upstream ORF SC2E9.17c, trkA, (E(): 1.8e-05, 22.6% identity in 212 aa overlap) 2 4 2 17 [Reporter]SCO7116 (5B21)_PCR/6589 [Gene]SCO7116/6002 NC_003888 SC4B10.17c, conserved hypothetical protein, len: 247 aa; highly similar to TR:Q9RD73 (EMBL:AL136502) Streptomyces coelicolor hypothetical 27.0 kD protein SCF43.15c, 247 aa; fasta scores: opt: 1219 z-score: 1467.5 E(): 0; 71.6% identity in 250 aa overlap 2 4 2 16 [Reporter]SCO2170 (6N17)_PCR/6588 [Gene]SCO2170/6001 NC_003888 SC5F7.31, possible methyltransferase, len: 233 aa; similar to SW:SMTA_ECOLI methyltransferase from Escherichia coli (261 aa) fasta scores; opt: 301, z-score: 359.0, E(): 1.2e-12, (31.1% identity in 212 aa overlap). 2 4 2 15 [Reporter]SCO2120 (7J17)_PCR/6587 [Gene]SCO2120/6000 NC_003888 SC6E10.14c, probable two-component system regulator, len: 215 aa; similar to many e.g. SW:DEGU_BACBR (EMBL:M21658), DegU, Bacillus brevis transcriptional regulatory protein for the production of extracellular proteases (236 aa), fasta scores; opt: 443 z-score: 510.2 E(): 4.3e-21, 36.5% identity in 219 aa overlap. Similar to others from S.coelicolor e.g. SCH10.18C (EMBL:AL049754) S.coelicolor probable two-component system regulator (228 aa) (37.9% identity in 214 aa overlap). Contains probable helix-turn-helix motif at aa 169-190 (Score 1764, +5.19 SD). Contains Pfam matches to entry PF00072 response_reg, Response regulator receiver domain and to entry PF00196 GerE, Bacterial regulatory proteins, luxR family. Contains PS00622 Bacterial regulatory proteins, luxR family signature 1 4 5 14 [Reporter]SCO6885 (8A21)_PCR/6148 [Gene]SCO6885/5599 NC_003888 SC7F9.37, possible DNA methylase, len: 251 aa. Similar to many methylases e.g. Moraxella bovis SW:MT1C_MORBO(EMBL:D13968) modification methylase MboI (EC 2.1.1.72) (273 aa), fasta scores opt:208 z-score: 250.5 E(): 1.5e-06 24.5% identity in 253 aa overlap and Streptomyces coelicolor TR:Q9X866(EMBL:AL049661) putative DNA methylase, SCE134.11c (248 aa), fasta scores opt: 976 z-score: 1145.8 E():0 57.6% identity in 250 aa overlap. Contains a Prosite hit to PS00092 N-6 Adenine-specific DNA methylases signature and a Pfam match to entry PF01555 N6_N4_Mtase, DNA methylase. 1 4 5 13 [Reporter]SCO6134 (9M17)_PCR/6147 [Gene]SCO6134/5598 NC_003888 SC9B2.21c, possible regulatory protein, len: 332aa; similar to many proposed regulators and hypotheticals eg. TR:O53314 (EMBL:AL021646) putative regulatory protein from Mycobacterium tuberculosis (320 aa) fasta scores; opt: 1227, z-score: 1374.2, E(): 0, (60.3% identity in 302 aa overlap). 1 4 5 12 [Reporter]SCO1948 (10I17)_PCR/6146 [Gene]SCO1948/5597 NC_003888 SCC54.08c, possible secreted zinc-binding carboxypeptidase, len: 999 aa; N-terminal region similar to many carboxypeptidases eg. SW:CBPS_STRGR zinc-carboxypeptidase from Streptomyces griseus (451 aa) fasta scores; opt: 565, z-score: 581.4, E(): 4.4e-25, (31.8% identity in 456 aa overlap). Contains PS00132 Zinc carboxypeptidases, zinc-binding region 1 signature and Pfam match to entry PF00246 Zn_carbOpept, Zinc carboxypeptidase, score 143.40, E-value 4e-39. Also contains probable N-terminal signal sequence. 1 4 5 11 [Reporter]SCO4358 (11E17)_PCR/6145 [Gene]SCO4358/5596 NC_003888 SCD19.13c, probable tetR family transcriptional regulatory protein, len: 268 aa; similar to TR:Q9S2B2 (EMBL:AL096849) Streptomyces coelicolor putative transcriptional regulator SCI11.07c, 234 aa; fasta scores: opt: 472 z-score: 546.8 E(): 6e-23; 40.2% identity in 229 aa overlap and to TR:Q53901 (EMBL:M64683) Streptomyces coelicolor ORF1-4 (actII), 259 aa; fasta scores: opt: 333 z-score: 387.9 E(): 4.2e-14; 32.1% identity in 243 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 56..77 (+3.34 SD) 1 4 5 10 [Reporter]SCO4274 (12A17)_PCR/6144 [Gene]SCO4274/5595 NC_003888 SCD95A.07c, possible secreted protein, len: 189 aa; similar to TR:CAA74718 (EMBL:Y14336) Streptomyces reticuli putative extracellular protein containing predicted 35 aa signal peptide precursor, 188 aa; fasta scores: opt: 773 z-score: 786.2 E(): 0; 66.3% identity in 187 aa overlap. Contains possible N-terminal region signal peptide sequence 1 4 5 9 [Reporter]SCO1838 (13M13)_PCR/6143 [Gene]SCO1838/5594 NC_003888 SCI8.23c, possible enoyl-CoA hydratase/isomerase, len: 267 aa. Similar to many including: Mycobacterium tuberculosis TR:P75019 (EMBL: Z80343) hypothetical 29.1 KD protein (274 aa), fasta scores opt: 331 z-score: 381.9 E(): 6.7e-14 33.9% identity in 236 aa overlap and Pseudomonas fluorescens TR:O69762 (EMBL: Y13067) P-hydroxycinnamoyl CoA hydratase/lyase (276 aa), fasta scores opt: 278 z-score: 322.1 E(): 1.4e-10 29.7% identity in 212 aa overlap. Contains a Pfam match to entry PF00378 ECH, Enoyl-CoA hydratase/isomerase family and a Prosite hit to PS00166 Enoyl-CoA hydratase/isomerase signature. 1 4 5 8 [Reporter]SCO0533 (14I13)_PCR/6142 [Gene]SCO0533/5593 NC_003888 SCF11.13, possible sugar transporter membrane protein, len: 285 aa; similar to TR:CAB46387 (EMBL:AL096743) Streptomyces coelicolor putative integral membrane transport protein MalG, 296 aa; fasta scores: opt: 538 z-score: 675.0 E(): 3.2e-30; 33.7% identity in 279 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, binding-protein-dependent transport systems inner membrane component and prosite match to entry PS00402 binding-protein-dependent transport systems inner membrane component signature 1 4 5 7 [Reporter]SCO3900 (15E13)_PCR/6141 [Gene]SCO3900/5592 NC_003888 SCH24.22c, conserved hypothetical protein, len: 225 aa; unknown function, similar to e.g. TR:P71704 (EMBL:Z80775) Mycobacterium tuberculosis hypothetical protein (180 aa), fasta scores; opt: 583 z-score: 671.1 E(): 4.5e-30, 51.4% identity in 177 aa overlap. Contains probable coiled-coil from 121 to 165 (45 residues) (Max score: 1.819, probability 1.00) 1 4 5 6 [Reporter]SCO1903 (16A13)_PCR/6140 [Gene]SCO1903/5591 NC_003888 SCI7.21, possible transport associated protein, len: 399aa; appears to be the last gene in a possible sugar transport operon, similar to many eg. TR:CAB38155 (EMBL:AL035591) putative export associated protein from Streptomyces coelicolor (192 aa) fasta scores; opt: 475, z-score: 388.5, E(): 2.6e-14, (43.6% identity in 188 aa overlap) and TR:CAA22211 (EMBL:AL034355) hypothetical protein from Streptomyces coelicolor (482 aa) fasta scores; opt: 534, z-score: 428.9, E(): 1.5e-16, (42.3% identity in 260 aa overlap) and SW:TERZ_SERMA tellurium resistance protein, TerZ from Serratia marcescens (193 aa) fasta scores; opt: 429, z-score: 352.8, E(): 2.6e-12, (47.6% identity in 185 aa overlap). 1 4 5 5 [Reporter]SCO1646 (17M9)_PCR/6139 [Gene]SCO1646/5590 NC_003888 SCI41.29c, hypothetical protein, len: 72 aa. Identical to TR:O87596 (EMBL:AF086832) Streptomyces coelicolor hypothetical 8.0 Kd protein, len: 72 aa and similar to TR:Q53082 (EMBL:U26422) Rhodococcus sp. similar to Rhodococcus sp. NI86/21, 64 aa; fasta scores: opt: 197 z-score: 237.9 E(): 7e-06; 51.4% identity in 70 aa overlap. Contains probable coiled-coil from 10 to 61 (52 residues, max score: 1.620, probability 1.00) 1 4 5 4 [Reporter]SCO3347 (18I9)_PCR/6138 [Gene]SCO3347/5589 NC_003888 SCE7.14c, unknown, len: 285aa; similar to SW:YV24_MYCTU hypothetical protein from Mycobacterium tuberculosis (280 aa) fasta scores; opt: 569, z-score: 688.7, E(): 4.9e-31, (54.6% identity in 273 aa overlap) 1 4 5 3 [Reporter]SCP1.254c (19E9)_PCR/6137 [Gene]SCP1.254c/5588 NC_003888 SCP1.254c, unknown, len: 207aa; 1 4 4 22 [Reporter]SCO4536 (1M1)_PCR/6134 [Gene]SCO4536/5587 NC_003888 2SCD4.07, hypothetical protein, len: 214 aa; similar to TR:CAB77303 (EMBL:AL160312) Streptomyces coelicolor hypothetical 21.2 kDa protein SCD35.19c, 193 aa; fasta scores: opt: 228 z-score: 264.4 E(): 2.9e-07; 31.1% identity in 212 aa overlap 1 4 4 21 [Reporter]SCO5371 (2I1)_PCR/6133 [Gene]SCO5371/5586 NC_003888 2SC6G5.15, atpA, ATP synthase alpha chain, len: 529aa; strongly similar to many eg. SW:P37808 (ATPA_BACSU) ATP synthase alpha chain from Bacillus subtilis (501 aa) fasta scores; opt: 1900, z-score: 2061.3, E(): 0, 58.1% identity in 513 aa overlap. Identical to SW:P50001 (ATPA_STRLI) ATP synthase alpha chain from Streptomyces lividans. Contains Pfam match to entry PF00422 ATP-synt_A-c, ATP synthase Alpha chain, C terminal, Pfam match to entry PF00006 ATP-synt_ab, ATP synthase alpha/beta family, Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) and Prosite match to PS00152 ATP synthase alpha and beta subunits signature 1 4 4 20 [Reporter]SCO4606 (3E1)_PCR/6132 [Gene]SCO4606/5585 NC_003888 SCD39.06, nuoL2, NADH dehydrogenase subunit, len: 664 aa; similar to SW:NU5C_SYNP2 (EMBL:M99378) Synechococcus sp. NADH-plastoquinone oxidoreductase chain 5 (EC 1.6.5.3) NdhF, 664 aa; fasta scores: opt: 997 z-score: 1098.6 E(): 0; 33.7% identity in 701 aa overlap and to TR:Q9XAR5 (EMBL:AL078618) Streptomyces coelicolor SCD16A.10c, NuoL, NADH dehydrogenase subunit, 654 aa; fasta scores: opt: 902 z-score: 801.3 E(): 0; 33.3% identity in 681 aa overlap. Contains Pfam matches to entries PF00662 oxidored_q1_N, NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus and PF00361 oxidored_q1, NADH-Ubiquinone/plastoquinone (complex I), various chains. Also contains possible hydrophobic membrane spanning regions 1 4 4 19 [Reporter]SCO6154 (4A1)_PCR/6131 [Gene]SCO6154/5584 NC_003888 SC1A9.18, unknown, len: 390 aa 1 4 4 18 [Reporter]SCO6228 (4M21)_PCR/6130 [Gene]SCO6228/5583 NC_003888 SC2H4.10, unknown, len: 204 aa 1 4 4 17 [Reporter]SCO7048 (5I21)_PCR/6129 [Gene]SCO7048/5582 NC_003888 SC4G1.14c, probable delta fatty acid desaturase, len: 347 aa; similar to TR:Q9X8W4 (EMBL:AL078610) Streptomyces coelicolor putative delta fatty acid desaturase SCH35.42c, 345 aa; fasta scores: opt: 1352 z-score: 1620.0 E(): 0; 60.1% identity in 343 aa overlap and to TR:Q9ZNW2 (EMBL:AJ22298) Physcomitrella patens delta 6-acyl-lipid desaturase Des6, 525 aa; fasta scores: opt: 425 z-score: 510.0 E(): 6.1e-21; 31.5% identity in 352 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 4 16 [Reporter]SCO7580 (6E21)_PCR/6128 [Gene]SCO7580/5581 NC_003888 SC5F1.34, hypothetical protein, len: 271 aa; similar to many Streptomyces coelicolor hypothetical proteins, e.g. TR:Q9WX26 (EMBL:AL079345) hypothetical 33.1 kDa protein SCE68.05c, 300 aa; fasta scores: opt: 794 z-score: 946.3 E(): 0; 51.6% identity in 248 aa overlap 1 4 4 15 [Reporter]SCO7416 (7A21)_PCR/6127 [Gene]SCO7416/5580 NC_003888 SC6D11.12c, unknown, len: 134 aa. 1 4 4 14 [Reporter]SCO2018 (8M17)_PCR/6126 [Gene]SCO2018/5579 NC_003888 SC7H2.32c, possible aminopeptidase, len: 835aa; similar to many eg. SW:AMPN_LACDL lysyl aminopeptidase from Lactobacillus delbruckii ssp. lactis (842 aa) fasta scores; opt: 705, z-score: 805.5, E(): 0, (27.7% identity in 622 aa overlap). Also similar to TR:O53194 (EMBL:AL021246) aminopeptidase from Mycobacterium tuberculosis (861 aa) fasta scores; opt: 1490, z-score: 1707.1, E(): 0, (39.6% identity in 867 aa overlap). Contains Pfam match to entry PF01433 Peptidase_M1, Peptidase family M1 and Prosite match to PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. 1 4 4 13 [Reporter]SCO6129 (9I17)_PCR/6125 [Gene]SCO6129/5578 NC_003888 SC9B2.16c, possible DNA-binding protein, len: 303aa; similar to proteins of unknown function egs. TR:O86676 (EMBL:AL031371) hypothetical 30.7 kD protein from Streptomyces coelicolor (277 aa) fasta scores; opt: 552, z-score: 663.3, E(): 1.2e-29, (37.7% identity in 257 aa overlap) and SW:ENTD_SALTY EntD, a protein from Salmonella typhimurium with undefined involvement in enterobactin biosynthesis (232 aa) fasta scores; opt: 102, z-score: 129.9, E(): 6, (26.7% identity in 191 aa overlap). Contains a probable helix-turn-helix (+3.37 SD) at residues 60 to 81 1 4 4 12 [Reporter]SCO2450 (10E17)_PCR/6124 [Gene]SCO2450/5577 NC_003888 SCC24.21, possible serine/threonine protein kinase (regulator), len: 1349 aa; N-terminal region similar to SW:PKAB_STRCO (EMBL:D86821) Streptomyces coelicolor serine/threonine protein kinase PkaB, 417 aa; blastp socres: Score = 109 (38.4 bits), Expect = 0.00070, Sum P(2) = 0.00070 Identities = 39/121 (32%), Positives = 60/121 (49%) and C-teminal region similar to SW:AFSR_STRCO (EMBL:D90155) Streptomyces coelicolor regulatory protein AfsR, 993 aa; fasta scores: opt: 510 z-score: 438.0 E(): 5.8e-17; 33.3% identity in 442 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain 1 4 4 11 [Reporter]SCO4354 (11A17)_PCR/6123 [Gene]SCO4354/5576 NC_003888 SCD19.09c, hypothetical protein, len: 227 aa; similar to TR:Q9RDD3 (EMBL:AL136503) Streptomyces coelicolor hypothetical 25.0 kDa protein SCC77.25c, 224 aa; fasta scores: opt: 234 z-score: 297.1 E(): 4.8e-09; 30.3% identity in 208 aa overlap 1 4 4 10 [Reporter]SCO3516 (12M13)_PCR/6122 [Gene]SCO3516/5575 NC_003888 SCE134.17, unknown, len: 150aa; 1 4 4 9 [Reporter]SCO5819 (13I13)_PCR/6121 [Gene]SCO5819/5574 NC_003888 SC5B8.09, whiH, sporulation transcription factor, le n: 295; almost identical to whiH protein TR:E1198707 (EMBL: AJ003022) (295 aa), 99.0% identity in 295 aa overlap. First of several possible start sites shown. Also similar to man y transcriptional regulators, e.g. DGOR_ECOLI P31460 galact onate operon transcriptional regulator (177 aa), fasta scor es; opt: 240 z-score: 251.2 E(): 8.5e-07, 30.1% identity in 173 aa overlap. Contains Pfam match to entry gntR PF00392, Bacterial regulatory proteins, gntR family, score 56.85 1 4 4 8 [Reporter]SCO1704 (14E13)_PCR/6120 [Gene]SCO1704/5573 NC_003888 SCI30A.25, possible integral membrane conserved hypothetical protein, len: 324aa; similar to many eg. SW:YDFC_BACSU hypothetical protein from Bacillus subtilis (306 aa) fasta scores; opt: 485, z-score: 529.4, E(): 3.7e-22, (34.2% identity in 269 aa overlap). Contains 2 Pfam matches to entry PF00892 DUF6, Integral membrane protein and several possible membrane spanning hydrophobic regions. 1 4 4 7 [Reporter]SCO0755 (15A13)_PCR/6119 [Gene]SCO0755/5572 NC_003888 SCF81.14, possible ABC transporter, len: 740 aa; similar to SW: COMA_STRPN (EMBL:M36180) Streptococcus pneumoniae transport ATP-binding protein ComA, 717 aa; fasta scores: opt: 1035 z-score: 1141.5 E(): 0; 29.1% identity in 721 aa overlap. Contains two matches to Pfam entries PF00664 ABC_membrane, ABC transporter transmembrane region and PF00005 ABC_tran, ABC transporter and two to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 4 2 10 15 [Reporter]SCO5577 (8L3)_PCR/3740 [Gene]SCO5577/3399 NC_003888 SC7A1.21, possible chromosome associated protein, len: 1186aa; similar to many hypotheticals eg. SW:Y06B_MYCTU hypothetical protein from Mycobacterium tuberculosis (1289 aa) fasta scores; opt: 2637, z-score: 2806.3, E(): 0, (53.1% identity in 1205 aa overlap) with weak similarity to a family of chromosome associated proteins e.g. SW:SCII_CHICK chromosome scaffold protein SCII from chicken (Gallus gallus) (1189 aa) opt: 370, z-score: 383.3, E(): 4.7e-14, (22.6% identity in 1246 aa overlap). Contains PS00017 ATP /GTP-binding site motif A (P-loop). Also contains 2 probable coiled coil regions from 162 to 492 and from 654 to 1041. 1 4 4 6 [Reporter]SCO3657 (16M9)_PCR/6118 [Gene]SCO3657/5571 NC_003888 SCH10.35c, hypothetical protein, len: 136 aa; unknown function, improbable Ala-rich CDS suggested by positional base preference 4 2 10 14 [Reporter]SCO7711 (9H3)_PCR/3739 [Gene]SCO7711/3398 NC_003888 SC8D11.02, possible two component system sensor kinase, len: 509 aa; similar to TR:Q53893 (EMBL:U51332) Streptomyces coelicolor AbsA1, 571 aa; fasta scores: opt: 852 z-score: 873.7 E(): 0; 42.1% identity in 489 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 4 5 [Reporter]SCO5040 (17I9)_PCR/6117 [Gene]SCO5040/5570 NC_003888 SCK7.13c, conserved hypothetical protein, len: 737 aa; similar to TR:Q9ZBJ0 (EMBL:AL035161) Streptomyces coelicolor conserved hypothetical protein SC9C7.20, 860 aa; fasta scores: opt: 1430 z-score: 1211.3 E(): 0; 37.5% identity in 787 aa overlap. Contains match to Prosite entry, TTA leucine codon, possible target for bldA regulation and 3x degenerate repeat (P/A)(D/G)GGQ 4 2 10 13 [Reporter]SCO5117 (10D3)_PCR/3738 [Gene]SCO5117/3397 NC_003888 SC9E12.02, possible peptide transport system secreted peptide-binding protein, len: 544 aa; similar to SW:OPPA_BACSU (EMBL:X56347) Bacillus subtilis oligopeptide-binding protein OppA precursor, 545 aa; fasta scores: opt: 648 z-score: 642.6 E(): 2.5e-28; 26.1% identity in 552 aa overlap and to TR:P72407 (EMBL:U68036) Streptomyces coelicolor SpoOKA homologue BldKB, 602 aa; blastp scores: Score = 165 (58.1 bits), Expect = 2.9e-10, Sum P(2) = 2.9e-10; Identities = 116/502 (23%), Positives = 202/502 (40%). Contains 2x Pfam matches to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5 and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 4 2 10 12 [Reporter]SCO2689 (10P23)_PCR/3737 [Gene]SCO2689/3396 NC_003888 SCC61A.10, possible membrane protein, len: 375aa; contains possible membrane-spanning hydrophobic regions. 4 2 10 11 [Reporter]SCO4432 (11L23)_PCR/3736 [Gene]SCO4432/3395 NC_003888 SCD6.10, unknown, len: 224 aa 4 2 10 10 [Reporter]SCO3502 (12H23)_PCR/3735 [Gene]SCO3502/3394 NC_003888 SCE134.03c, possible 3-oxoacyl-[acyl carrier protein] reductase, len: 255 aa; similar to many e.g. SW:FABG_BACSU 3-oxoacyl-[acyl carrier protein] reductase from Bacillus subtilis (246 aa) fasta scores; opt: 522, z-score: 518.3, E(): 1.5e-21, (38.1% identity in 247 aa overlap). Contains Pfam matches to entries PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and PF00106 adh_short, short chain dehydrogenase. 4 2 10 9 [Reporter]SCO0947 (13D23)_PCR/3734 [Gene]SCO0947/3393 NC_003888 SCM11.02, possible integral membrane protein, len: 470 aa; similar to TR:P96418 (EMBL:Z92669) Mycobacterium tuberculosis hypothetical 54.8 kD protein MTCY08D5.31c, 482 aa; fasta scores: opt: 2112 z-score: 2527.5 E(): 0; 61.9% identity in 472 aa overlap and to TR:O69633 (EMBL:AL022121) Mycobacterium tuberculosis putative peptide transport system permease; fasta scores: opt: 122 z-score: 149.8 E(): 0.58; 27.7% identity in 166 aa overlap 4 2 10 8 [Reporter]SCO2959 (14P19)_PCR/3733 [Gene]SCO2959/3392 NC_003888 SCE59.18c, probable nitrate extrusion protein, len: 452 aa; similar to SW:NARK_ECOLI (EMBL:X15996) Escherichia coli nitrite extrusion protein 1 (nitrite facilitator 1) NarK, 463 aa; fasta scores: opt: 526 z-score: 590.8 E(): 1.7e-25; 39.7% identity in 451 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 10 7 [Reporter]SCO0179 (15L19)_PCR/3732 [Gene]SCO0179/3391 NC_003888 SCJ1.28c,possible zinc-containing dehydrogenase, len: 356 aa. Similar to many including: Thermoanaerobacter brockii SW:ADH_THEBR (EMBL; X64841) NADP-dependent alcohol dehydrogenase (EC 1.1.1.2) (352 aa), fasta scores opt: 556 z-score: 601.1 E(): 4e-26 33.7% identity in 338 aa overlap and Halobacterium volcanii TR:O34179 (EMBL; U95372) dehydrogenase (389 aa), fasta scores opt: 715 z-score: 770.1 E(): 0 37.0% identity in 351 aa overlap. Contains a Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and a PS00059 Zinc-containing alcohol dehydrogenases signature 4 2 10 6 [Reporter]SCO0394 (16H19)_PCR/3731 [Gene]SCO0394/3390 NC_003888 SCF62.20, unknown, len: 215 aa 1 4 4 4 [Reporter]SCO1771 (18E9)_PCR/6116 [Gene]SCO1771/5569 NC_003888 SCI51.11c, hypothetical protein, len: 185 aa; unknown function, improbable CDS suggested by positional base preference and amino acid composition 1 4 4 3 [Reporter]SCO1679 (19A9)_PCR/6115 [Gene]SCO1679/5568 NC_003888 SCI52.21, probable gluconokinase, len: 175 aa; similar to SW:IDNK_ECOLI (EMBL:U14003) Escherichia coli thermosensitive gluconokinase (EC 2.7.1.12) IdnK or GntV or B4268, 187 aa; fasta scores: opt: 434 Z-score: 502.9 bits: 99.6 E(): 2e-20; 43.125% identity in 160 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 4 4 2 [Reporter]SCO1676 (20I5)_PCR/6114 [Gene]SCO1676/5567 NC_003888 SCI52.18c, possible peptidase, len: 445 aa; similar to TR:Q9CC46 (EMBL:AL583921) Mycobacterium leprae possible peptidase ML1288, 467 aa; fasta scores: opt: 1595 Z-score: 1853.9 bits: 352.3 E(): 1.1e-95; 54.788% identity in 449 aa overlap, to SW:ACY1_PIG (EMBL:D13514) Sus scrofa aminoacylase-1 (EC 3.5.1.14) Acy1, 406 aa; fasta scores: opt: 368 Z-score: 430.7 bits: 88.7 E(): 2.1e-16; 27.563% identity in 439 aa overlap and to TR:Q9ZBI7 (EMBL:AL035161) Streptomyces coelicolor putative aminoacylase SC9C7.23, 444 aa; fasta scores: opt: 1543 Z-score: 1530.3 bits: 292.3 E(): 1.6e-79; 54.795% identity in 438 aa overlap. Contains Pfam match to entry PF01546 Peptidase_M20, Peptidase family M20/M25/M40 and match to Prosite entry PS00758 ArgE / dapE / ACY1 / CPG2 / yscS family signature 1 1 4 3 22 [Reporter]SCO4532 (1I1)_PCR/6112 [Gene]SCO4532/5566 NC_003888 2SCD4.03, possible membrane protein, len: 153 aa. Contains possible hydrophobic membrane spanning region 1 4 3 21 [Reporter]SCO0724 (2E1)_PCR/6111 [Gene]SCO0724/5565 NC_003888 3SC5B7.02, probable transcriptional regulator, len: 313 aa; similar to TR:Q9KZ73 (EMBL:AL354048) Streptomyces coelicolor putative transcriptional regulator SCE25.16c, 289 aa; fasta scores: opt: 721 z-score: 810.2 E(): 0; 43.9% identity in 289 aa overlap and to SW:HEXR_PSEAE (EMBL:AF029673) Pseudomonas aeruginosa hex regulon repressor HexR, 285 aa; fasta scores: opt: 167 z-score: 194.4 E(): 0.0027; 26.8% identity in 224 aa overlap. Contains a possible coiled-coil region at approx. residues 125..147 and a possible helix-turn-helix motif at residues 69..90 (+5.16 SD) 1 4 3 20 [Reporter]SCO4604 (3A1)_PCR/6110 [Gene]SCO4604/5564 NC_003888 SCD39.04, nuoJ2, NADH dehydrogenase subunit, len: 197 aa; similar to SW:NU6C_SYNY3 (EMBL:X62517) Synechocystis sp. NADH-plastoquinone oxidoreductase chain 6 (EC 1.6.5.3) NdhG, 198 aa; fasta scores: opt: 311 z-score: 383.0 E(): 7.2e-14; 35.7% identity in 185 aa overlap and to TR:Q9XAR3 (EMBL:AL078618) Streptomyces coelicolor SCD16A.12c NuoJ, NADH dehydrogenase subunit, 285 aa; fasta scores: opt: 278 z-score: 288.5 E(): 2e-10; 33.1% identity in 181 aa overlap. Contains Pfam match to entry PF00499 oxidored_q3, NADH-ubiquinone/plastoquinone oxidoreductase chain 6 and possible hydrophobic membrane spanning regions 1 4 3 19 [Reporter]SCO1395 (3M21)_PCR/6109 [Gene]SCO1395/5563 NC_003888 SC1A8A.15c, mutT-like protein, len: 138 aa; similar to many eg. SW:P08337 (MUTT_ECOLI) mutator mutT protein from Escherichia coli (129 aa) fasta scores; opt: 131, z-score: 177.0, E(): 0.02, 33.7% identity in 101 aa overlap. Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein and Prosite match to PS00893 mutT domain signature. 1 4 3 18 [Reporter]SCO6224 (4I21)_PCR/6108 [Gene]SCO6224/5562 NC_003888 SC2H4.06c, probable secreted protein, len: 271 aa; contains N-terminal signal sequence 4 2 10 5 [Reporter]SCO3741 (17D19)_PCR/3730 [Gene]SCO3741/3389 NC_003888 SCH22A.19c, probable response regulator, len: 256 aa; similar to TR:P74922 (EMBL:U67196) Thermotoga maritima DNA-binding response regulator DrrA, 239 aa; fasta scores: opt: 664 z-score: 686.2 E(): 8.4e-31; 45.3% identity in 234 aa overlap and to SW:AFQ1_STRCO (EMBL:D10654) Streptomyces coelicolor transcriptional regulatory protein AfsQ1, 225 aa; fasta scores: opt: 630 z-score: 652.1 E(): 6.7e-29; 47.1% identity in 221 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal. Contains also possible N-terminal region signal peptide sequence 1 4 3 17 [Reporter]SCO7119 (5E21)_PCR/6107 [Gene]SCO7119/5561 NC_003888 SC4B10.20, probable dehydrogenase, len: 312 aa; similar to SW:SERA_METJA (EMBL:U67544) Methanococcus jannaschii D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) SerA, 524 aa; fasta scores: opt: 407 z-score: 451.9 E(): 1e-17; 31.6% identity in 266 aa overlap and to TR:CAB37585 (EMBL:AL035569) Streptomyces coelicolor putative oxidoreductase SC8D9.21c, 313 aa; fasta scores: opt: 1071 z-score: 988.6 E(): 0; 56.0% identity in 307 aa overlap. Contains Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases and match to Prosite entry PS00671 D-isomer specific 2-hydroxyacid dehydrogenases signature 3 4 2 10 4 [Reporter]SCO6098 (18P15)_PCR/3729 [Gene]SCO6098/3388 NC_003888 SCBAC1A6.22c, cysD, sulfate adenylyltransferase subunit 2, len: 312 aa; highly similar to SW:MMCV_STRLA (EMBL:AF127374) Streptomyces lavendulae sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) MmcV, 319 aa; fasta scores: opt: 1734 z-score: 2027.7 E(): 0; 81.6% identity in 309 aa overlap and to SW:CYSD_ECOLI (EMBL:M74586) Escherichia coli sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) CysD, 302 aa; fasta scores: opt: 909 z-score: 1066.6 E(): 0; 47.7% identity in 298 aa overlap. Contains Pfam match to entry PF01507 PAPS_reduct, Phosphoadenosine phosphosulfate reductase family 1 4 3 16 [Reporter]SCO7576 (6A21)_PCR/6106 [Gene]SCO7576/5560 NC_003888 SC5F1.30c, probable secreted hydrolase, len: 397 aa; highly similar to TR:Q9Z4I2 (EMBL:AB019428) Streptomyces matensis laminaripentaose-producing beta-1,3-guluase (LPHase) precursor LPH, 401 aa; fasta scores: opt: 1827 z-score: 1870.8 E(): 0; 65.0% identity in 394 aa overlap and to Streptomyces coelicolor SC5F1.13c, 398 aa; fasta scores: opt: 1795 z-score: 1665.4 E(): 0; 66.6% identity in 386 aa overlap. Contains possible N-terminal region signal peptide sequence 4 2 10 3 [Reporter]SCO1378 (19L15)_PCR/3728 [Gene]SCO1378/3387 NC_003888 SC10A9.20c, probable glycine dehydrogenase, len: 961 aa; similar to SW:GCSP_ECOLI (EMBL:L20872) Escherichia coli glycine dehydrogenase [decarboxylating] (EC 1.4.4.2) GcvP, 956 aa; fasta scores: opt: 3621 z-score: 3847.9 E(): 0; 56.5% identity in 960 aa overlap. Contains Pfam match to entry PF02347 GDC-P 4 2 10 2 [Reporter]SCO5110 (20D15)_PCR/3727 [Gene]SCO5110/3386 NC_003888 SCBAC31E11.06, possible lipoprotein, len: 771 aa; similar to SW:YC80_MYCTU (EMBL:Z77137) Mycobacterium tuberculosis hypothetical 63.5 kDa protein MTCY50.02, 591 aa; fasta scores: opt: 565 Z-score: 501.6 bits: 103.2 E(): 2.5e-20; 26.190% identity in 756 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 2 9 22 [Reporter]SCO2798 (1D11)_PCR/3725 [Gene]SCO2798/3385 NC_003888 2SCC13.06, probable cellobiose hydrolase, len: 479 aa; highly similar to TR:Q59976 (EMBL:Z29625) Streptomyces sp. beta-glucosidase (EC 3.2.1.21) (cellobiose) Bgl3, 479 aa; fasta scores: opt: 2214 z-score: 2511.6 E(): 0; 65.5% identity in 472 aa overlap. Contains Pfam match to entry PF00232 Glyco_hydro_1, Glycosyl hydrolase family 1 and matches to Prosite entries PS00653 Glycosyl hydrolases family 1 N-terminal signature and PS00572 Glycosyl hydrolases family 1 active site 4 2 9 21 [Reporter]SCO0190 (2P7)_PCR/3724 [Gene]SCO0190/3384 NC_003888 SCJ12.02c, crtT, probable methyltransferase, len: 246 aa. Shares a high level of sequence similarity with Streptomyces griseus TR:P72450 (EMBL:X95596) methyltransferase (242 aa), fasta scores opt: 984 z-score: 1164.2 E(): 0 65.0% identity in 234 aa overlap. Contains a Pfam match to entry PF01209 Ubie_methyltran, ubiE/COQ5 methyltransferase family 4 2 9 20 [Reporter]SCO4715 (3L7)_PCR/3723 [Gene]SCO4715/3383 NC_003888 SCD31.40, rpsN, 30S ribosomal protein S14, len: 61 aa; highly similar to SW:RS14_BACST Bacillus stearothermophilus 30S ribosomal protein S14 RpsN, 61 aa; fasta scores: opt: 335 z-score: 483.7 E(): 1.6e-19; 68.9% identity in 61 aa overlap. Contains Pfam match to entry PF00253 Ribosomal_S14, Ribosomal protein S14p/S29e and match to Prosite entry PS00527 Ribosomal protein S14 signature 4 2 9 19 [Reporter]SCO5521 (4H7)_PCR/3722 [Gene]SCO5521/3382 NC_003888 SC1C2.02, questionable ORF, len: 43 aa 4 2 9 18 [Reporter]SCO5731 (5D7)_PCR/3721 [Gene]SCO5731/3381 NC_003888 SC3C3.17c, possible secreted serine protease, len: 450 aa; similar in part to many e.g. SUBT_BACSU subtilisin E precursor (381 aa), fasta scores; opt: 321 z-score: 359.3 E(): 9.4e-13, 32.3% identity in 294 aa overlap. Also similar to downstream serine protease, SC3C3.08 (E(): 7.1e-07, 35.0% identity in 383 aa overlap). Contains N-terminal signal sequence, possible transmembrane domain at C-terminus and Pfam match to entry PF00082 subtilase, Subtilase family of serine proteases, score 151.00, E-value 2.1e-41 4 2 9 17 [Reporter]SCO6670 (6P3)_PCR/3720 [Gene]SCO6670/3380 NC_003888 SC5A7.20, probable glucosidase, len: 441 aa; similar to e.g. CELF_ECOLI 6-phospho-beta-glucosidase (450 aa), fasta scores; opt: 537 z-score: 628.7 E(): 8.8e-28, 31.8% identity in 456 aa overlap 1 4 3 15 [Reporter]SCO2123 (7M17)_PCR/6105 [Gene]SCO2123/5559 NC_003888 SC6E10.17, possible esterase/lipase, len: 267 aa; similar to esterases e.g. SW:EST_BACST (EMBL:D12681), Est, Bacillus stearothermophilus carboxylesterase precursor (247 aa), fasta scores; opt: 333 z-score: 387.6 E(): 2.9e-14, 32.7% identity in 251 aa overlap 1 4 3 14 [Reporter]SCO2014 (8I17)_PCR/6104 [Gene]SCO2014/5558 NC_003888 SC7H2.28c, pyk1, pyruvate kinase, len: 478 aa; strongly similar to many e.g. SW:KPYK_CORGL pyruvate kinase from Corynebacterium glutamicum (475 aa) fasta scores; opt: 1800, z-score: 2008.1, E(): 0, (59.6% identity in 473 aa overlap). Contains Pfam match to entry PF00224 PK, Pyruvate kinase and Prosite match to PS00110 Pyruvate kinase active site signature. 1 4 3 13 [Reporter]SCO5742 (9E17)_PCR/6103 [Gene]SCO5742/5557 NC_003888 SC9A10.06c, putative membrane protein, len: 79 aa. Contains possible hydrophobic membrane spanning region 1 4 3 12 [Reporter]SCO2446 (10A17)_PCR/6102 [Gene]SCO2446/5556 NC_003888 SCC24.17c, probable secreted peptidase, len: 1220 aa; similar to TR:P95684 (EMBL:D83672) Streptomyces albogriseolus subtilisin-like protease, 1102 aa; fasta scores: opt: 1362 z-score: 1378.8 E(): 0; 33.7% identity in 1233 aa overlap and to TR:CAB56662 (EMBL:AL121596) Streptomyces coelicolor probable secreted peptidase SCF51A.10, 1245 aa; fasta scores: opt: 1509 z-score: 1527.2 E(): 0; 39.5% identity in 1278 aa overlap. Contains Pfam match to entry PF00082 Peptidase_S8, Subtilase family and matches to Prosite entries PS00136 Serine proteases, subtilase family, aspartic acid active site, S00137 Serine proteases, subtilase family, histidine active site and PS00138 Serine proteases, subtilase family, serine active site. Contains also possible N-terminal region signal peptide sequence 1 4 3 11 [Reporter]SCO4697 (11M13)_PCR/6101 [Gene]SCO4697/5555 NC_003888 SCD31.22, possible integral membrane protein, len: 604 aa; N-terminal region similar to TR:Q9XD98 (EMBL:AF106004) Streptomyces coelicolor hypothetical 27.5 kD protein, 249 aa; fasta scores: opt: 953 z-score: 1009.5 E(): 0; 58.7% identity in 235 aa overlap and C-terminal region highly similar to C-terminal region of TR:O86757 (EMBL:AL031035) Streptomyces coelicolor hypothetical 28.2 kD protein SC6A9.25, 254 aa; fasta scores: opt: 309 z-score: 332.3 E(): 4.4e-11; 92.0% identity in 50 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop), a possible hydrophobic membrane spanning region and a possible coiled-coil region at aprox residues 170..210 1 4 3 10 [Reporter]SCO3511 (12I13)_PCR/6100 [Gene]SCO3511/5554 NC_003888 SCE134.12, possible lipoprotein, len: 381aa; similar to many, mostly hypotheticals, eg. TR:P71594 (EMBL:Z80233) hypothetical protein from Mycobacterium tuberculosis (281 aa) fasta scores; opt: 288, z-score: 295.1, E(): 4e-09, (40.9% identity in 132 aa overlap). Also similar to SW:SPR_ECOLI lipoprotein Spr precusror from Escherichia coli (188 aa) fasta scores; opt: 201, z-score: 211.7, E(): 0.00018, (37.2% identity in 113 aa overlap). Contains a possible N-terminal signal sequence and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family domain of unknown function. 1 4 3 8 [Reporter]SCO3568 (14A13)_PCR/6098 [Gene]SCO3568/5553 NC_003888 SCH17.02c, conserved hypothetical protein, len: 247 aa; similar to hypothetical proteins from several organisms e.g. TR:O69640 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical protein (273 aa), fasta scores; opt: 524 z-score: 559.0 E(): 8.3e-24, 42.6% identity in 251 aa overlap. Similar sequences from other organisms e.g. Escherichia coli, Klebsiella aerogenes and Schizosaccharomyces pombe, have N-termini divergent from this sequence 1 4 3 7 [Reporter]SCO3218 (15M9)_PCR/6097 [Gene]SCO3218/5552 NC_003888 SCE8.11c, small conserved hypothetical protein, len: 71aa; similar to others located within antibiotic synthesis clusters egs. TR:O52824 (EMBL:AJ223999) hypothetical protein located within the vancomycin biosynthesis cluster in Amycolatopsis orientalis (56 aa) fasta scores; opt: 281, z-score: 402.6, E(): 4e-15, (67.3% identity in 52 aa overlap) and SW:YBDZ_ECOLI hypothetical protein located within the enterobactin region of Escherichia coli (72 aa) fasta scores; opt: 186, z-score: 272.4, E(): 7.3e-08, (32.8% identity in 64 aa overlap). 4 2 9 16 [Reporter]SCO3613 (7L3)_PCR/3719 [Gene]SCO3613/3379 NC_003888 SC66T3.24c, possible RNA polymerase sigma factor, len: 190 aa; weakly similar to many e.g. SW:RPOE_STRCO (EMBL:L29636), AigE, S.coelicolor RNA polymerase sigma-E factor (176 aa), fasta scores; opt: 236 z-score: 274.0 E(): 6.1e-08, 30.9% identity in 162 aa overlap. The start site is uncertain as there is a second possible start site at codon 7 1 4 3 6 [Reporter]SCO3904 (16I9)_PCR/6096 [Gene]SCO3904/5551 NC_003888 SCH24.26c, conserved hypothetical protein, len: 373 aa; weakly similar to many e.g. SW:FEMA_STAAU (EMBL:M23918), femA, Staphylococcus aureus factor essential for expression of methicillin resistance (433 aa), fasta scores; opt: 169 z-score: 200.3 E(): 0.00076, 21.7% identity in 428 aa overlap 4 2 9 15 [Reporter]SCO6685 (8H3)_PCR/3718 [Gene]SCO6685/3378 NC_003888 SC5A7.35c, ramR, two-component system response regulator, len: >41 aa. Identical to TR:Q53820 (EMBL:U03771) potential response regulator for aerial mycelium production RamR (202 aa), and similar to many e.g. Streptomyces peucetius TR:Q54821 (EMBL:L37338) daunorubicin biosynthesis regulatory protein dnrN (202 aa), fasta scores; opt: 173 z-score: 382.4 E(): 4.6e-14, 65.0% identity in 40 aa overlap,SC6G3.01c, ramR, probable two-component system response regulator, partial CDS, len: >201 aa; previously sequenced as TR:Q53820 (EMBL:U03771), RamR, potential response regulator for aerial mycelium production. Similar to many two-component system transcriptional regulators e.g. TR:Q07640 (EMBL:AB006206), AfmR, Streptomyces griseus transcriptional regulator involved in aerial mycelium formation (210 aa), fasta scores; opt: 466 z-score: 545.2 E(): 4.9e-23, 42.6% identity in 202 aa overlap. An alternative start codon is present at codon 4. Contains probable helix-turn-helix motif at aa 158-179 (Score 1876, +5.58 SD). Contains Pfam match to PF00196 GerE, Bacterial regulatory proteins, luxR family 1 4 3 5 [Reporter]SCO0823 (17E9)_PCR/6095 [Gene]SCO0823/5550 NC_003888 SCF43A.13, probable gntR-family transcriptional regulator, len: 146 aa; weakly similar to transcriptional regulators e.g. TR:Q07191 (EMBL:Z19589), KorSA, Streptomyces ambofaciens plasmid pSAM2 transcriptional regulator protein (259 aa), fasta scores; opt: 159 z-score: 203.8 E(): 0.00051, 32.3% identity in 99 aa overlap. Similar to SW:YHCF_BACSU (EMBL:X96983) Bacillus subtilis hypothetical transcriptional regulator (121 aa) (36.8% identity in 106 aa overlap). Weakly similar to others from S.coelicolor e.g. SCGD3.13 on cosmid GD3, probable gntR-family transcriptional regulator (115 aa) (32.1% identity in 112 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 4 2 9 14 [Reporter]SCO5670 (9D3)_PCR/3717 [Gene]SCO5670/3377 NC_003888 SC8B7.08c, probable polyamine ABC-transporter integr al membrane protein, len: 266 aa; similar to many e.g. POTC _ECOLI spermidine/putrescine transport system permease (264 aa), fasta scores; opt: 579 z-score: 718.8 E(): 8.8e-33, 3 5.5% identity in 251 aa overlap. Contains Pfam match to ent ry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 23.00, E-value 0.00 7 4 2 9 13 [Reporter]SCO6465 (9P23)_PCR/3716 [Gene]SCO6465/3376 NC_003888 SC1A11.02c, partial CDS, unknown, len: 134aa; N-terminal region similar to TR:O27370 (EMBL:AE000895) proposed mutator MutT protein from Methanobacterium thermoautotrophicum (135 aa) fasta scores; opt: 148, z-score: 219.8, E(): 5.9e-05, (43.5% identity in 62 aa overlap).,SC9C7.01c, partial CDS, unknown, len: 49aa; overlapping extreme N-terminal region of partial CDS, SC1A11.2c from Streptomyces coelicolor cosmid 1A11. 4 2 9 12 [Reporter]SCO2683 (10L23)_PCR/3715 [Gene]SCO2683/3375 NC_003888 SCC61A.04c, possible single-strand DNA-binding protein, len: 156 aa; similar to many eg. SW:P71711 (SSB_MYCTU) single-strand binding protein from Mycobacterium tuberculosis (164 aa) fasta scores; opt: 259, z-score: 301.8, E(): 2.4e-09, 35.5% identity in 141 aa overlap and TR:Q9X8U3 (EMBL:AL049826) putative single-strand binding protein from Streptomyces coelicolor (199 aa) fasta scores; opt: 261, z-score: 302.7, E(): 2.1e-09, 42.5% identity in 120 aa overlap. Contains Pfam match to entry PF00436 SSB, Single-strand binding protein family. 4 2 9 11 [Reporter]SCO4680 (11H23)_PCR/3714 [Gene]SCO4680/3374 NC_003888 SCD31.05c, possible DNA-binding protein, len: 296 aa; similar to TR:CAB53273 (EMBL:AL109972) Streptomyces coelicolor putative DNA binding protein SCJ9A.12c, 279 aa; fasta scores: opt: 782 z-score: 910.0 E(): 0; 46.1% identity in 271 aa overlap 4 2 9 10 [Reporter]SCO3393 (12D23)_PCR/3713 [Gene]SCO3393/3373 NC_003888 SCE126.11, possible integral membrane protein, len: 151aa; similar to other hypotheticals eg. TR:P96615 (EMBL:AB001488) hypothetical protein from Bacillus subtilis (159 aa) fasta scores; opt: 208, z-score: 264.3, E(): 2.1e-07, (30.3% identity in 145 aa overlap). Contains a possible membrane spanning hydrophobic region. 3 1 14 18 [Reporter]SCO5464 (5G12)_PCR/1311 [Gene]SCO5464/1199 NC_003888 SC3D11.21, possible calcium-binding protein, len: 70aa; similar to many eukaryotic calcium-binding proteins eg. TR:Q42470 (EMBL:D63153) calcium-binding protein from Brassica rapa (Turnip), and Brassica napus (Rape) (79 aa) fasta scores; opt: 153, z-score: 214.7, E(): 0.00015, 45.3% identity in 64 aa overlap and SW:P02599 (CALM_DICDI) calmodulin from Dictyostelium discoideum (Slime mold) (151 aa) fasta scores; opt: 155, z-score: 213.0, E(): 0.00019, 43.3% identity in 60 aa overlap. Contains Pfam match to entry PF00036 efhand, EF hand and two Prosite matches to PS00018 EF-hand calcium-binding domain. 4 2 9 9 [Reporter]SCO5173 (13P19)_PCR/3712 [Gene]SCO5173/3372 NC_003888 SCP8.36, hypothetical protein, len: 559 aa; low similarity to TR:Q9XAG2 (EMBL:AL079356) Streptomyces coelicolor possible membrane protein SC6G9.20, 303 aa; fasta scores: opt: 452 z-score: 362.9 E(): 9.5e-13; 34.1% identity in 258 aa overlap 3 1 14 17 [Reporter]SCO5807 (6C12)_PCR/1310 [Gene]SCO5807/1198 NC_003888 SC4H2.28, unknown, len: 225 aa; start site uncertain 4 2 9 8 [Reporter]SCO1463 (14L19)_PCR/3711 [Gene]SCO1463/3371 NC_003888 SCL6.20c, probable transcriptional regulator, len: 360 aa; similar to SW:DEOR_BACSU (EMBL:X82174) Bacillus subtilis deoxyribonucleoside regulator DeoR, 313 aa ;fasta scores: opt: 363 z-score: 408.8 E(): 2.4e-15; 28.0% identity in 307 aa overlap. Contains possible helix-turn-helix motif at residues 54..75 (+4.15 SD) 3 1 14 16 [Reporter]SCO6772 (7O8)_PCR/1309 [Gene]SCO6772/1197 NC_003888 SC6A5.21c, unknown, len: 287aa; similar to TR:P96830 (EMBL:Z92770) hypothetical protein from Mycobacterium tuberculosis (276 aa) fasta scores; opt: 189, z-score: 220.4, E(): 5.6e-05, (32.3% identity in 279 aa overlap). 4 2 9 7 [Reporter]SCO3871 (15H19)_PCR/3710 [Gene]SCO3871/3370 NC_003888 SCH18.08, possible decarboxylase, len: 136 aa; similar to TR:O26336 (EMBL:AE000810) Methanobacterium thermoautotrophicum gamma-carboxymuconolactone decarboxylase Mth234, 125 aa; fasta scores: opt: 231 z-score: 310.2 E(): 8.3e-10; 38.3% identity in 107 aa overlap 3 1 14 15 [Reporter]SCO2482 (8K8)_PCR/1308 [Gene]SCO2482/1196 NC_003888 SC7A8.21, possible dehydrogenase (putative secreted protein), len: 311 aa; similar to various hypothetical dehydrogenases, e.g. TR:Q9YE14 (EMBL:AP000060) Aeropyrum pernix 287 aa long hypothetical 3-hydroxyisobutyrate dehydrogenase APE0756, 287 aa; fasta scores: opt: 196 z-score: 223.5 E(): 4.7e-05; 27.0% identity in 293 aa overlap. Contains possible N-terminal region signal peptide sequence 3 1 14 14 [Reporter]SCO5491 (9G8)_PCR/1307 [Gene]SCO5491/1195 NC_003888 SC8D9.03, unknown, len: 182aa; similar to many of undefined function eg. TR:Q49952 (EMBL:U15180) hypothetical protein from Mycobacterium leprae (187 aa) fasta scores; opt: 331, z-score: 394.0, E(): 1.2e-14, (36.1% identity in 166 aa overlap). 3 1 14 12 [Reporter]SCO5282 (11O4)_PCR/1305 [Gene]SCO5282/1194 NC_003888 SCCB12.06c, possible two-component system sensor kinase, len: 375 aa; similar to SW:AFQ2_STRCO (EMBL:D10654) Streptomyces coelicolor sensor protein AfsQ2, 535 aa; fasta scores: opt: 344 z-score: 382.3 E(): 7.9e-14; 32.5% identity in 335 aa overlap. Contains Pfam matches to entries PF00672 DUF5, HAMP domain and PF00512 signal, Histidine kinase. Also contains possible hydrophobic membrane spanning regions 3 1 14 11 [Reporter]SCO3935 (12K4)_PCR/1304 [Gene]SCO3935/1193 NC_003888 SCD78.02, small hydrophobic protein, len: 65 aa; within putative integrated plasmid. Contains possible hydrophobic membrane spanning regions 3 1 14 10 [Reporter]SCO3204 (13G4)_PCR/1303 [Gene]SCO3204/1192 NC_003888 SCE22.21c, unknown, len: 253 aa; identical to previously sequenced TR:Q54224 (EMBL:X79980) Streptomyces griseus unknown gene, 253 aa and similar to SW:YGID_ECOLI (EMBL:M77129) Escherichia coli hypothetical 29.9 kD protein in TolC-RibB intergenic region (ORFC) YgiD, 271 aa; fasta scores: opt: 566 z-score: 655.8 E(): 4.6e-29; 38.5% identity in 262 aa overlap 3 1 14 9 [Reporter]SCO3324 (14C4)_PCR/1302 [Gene]SCO3324/1191 NC_003888 SCE68.22, hypothetical protein, len: 419 aa; unknown function, probable serine-rich CDS suggested by positional base preference, GC frame analysis and amino acid composition. An alternative start codon is present at codon 4 3 1 14 8 [Reporter]SCO1462 (14O24)_PCR/1301 [Gene]SCO1462/1190 NC_003888 SCL6.19c, unknown, len: 117 aa 1 4 3 4 [Reporter]SCO0404 (18A9)_PCR/6094 [Gene]SCO0404/5549 NC_003888 SCF51.03, possible 2-haloalkanoic acid dehalogenase, len: 246 aa; similar to SW:HAD2_PSEPU (EMBL:D17523) Pseudomonas putida 2-haloalkanoic acid dehalogenase H-109 (EC 3.8.1.2) (L-2-haloacid dehalogenase H-109) (halocarboxylic acid halidohydrolase H-109) DehH109, 224 aa: fasta scores: opt: 275 z-score: 328.4 E(): 6.4e-11; 32.2% identity in 202 aa overlap. Contains a Pfam match to entry PF00702 hydrolase, haloacid dehalogenase-like hydrolase 1 4 3 3 [Reporter]SCP1.77c (19M5)_PCR/6093 [Gene]SCP1.77c/5548 NC_003888 SCP1.77c, possible membrane protein, len: 220aa; note possible downstream alternative translational start sites. Contains possible membrane-spanning hydrophobic region in the N-terminal. 1 4 3 2 [Reporter]SCO4011 (20E5)_PCR/6092 [Gene]SCO4011/5547 NC_003888 2SC10A7.15, possible integral membrane protein, len: 157 aa; identical to TR:Q04915 (EMBL:X70689) Streptomyces lividans ORF904 protein, 157 aa. Contains possible hydrophobic membrane spanning regions 1 4 2 22 [Reporter]SCO5181 (1E1)_PCR/6090 [Gene]SCO5181/5546 NC_003888 2SC3B6.05c, possible integral membrane protein, len: 925 aa; similar to TR:O53781 (EMBL:AL021942) Mycobacterium tuberculosis hypothetical 84.0 kD protein RV0585c, 795 aa; fasta scores: opt: 618 z-score: 636.0 E(): 5.9e-28; 28.1% identity in 884 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 2 21 [Reporter]SCO4915 (2A1)_PCR/6089 [Gene]SCO4915/5545 NC_003888 SCK13.07, possible integral membrane protein, len: 234 aa. Contains 3 possible hydrophobic membrane spanning regions 1 4 2 20 [Reporter]SCO6534 (2M21)_PCR/6088 [Gene]SCO6534/5544 NC_003888 SC5C7.19c, narH, probable nitrate reductase beta chain, len: 531 aa; highly similar to many e.g. NARH_BACSU nitrate reductase beta chain (EC 1.7.99.4) (487 aa), fasta scores; opt: 2099 z-score: 2179.2 E(): 0, 58.0% identity in 495 aa overlap. Contains PS00190 Cytochrome c family heme-binding site signature and Pfam match to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains, score 61.10, E-value 1.8e-16 1 4 2 19 [Reporter]SCO1390 (3I21)_PCR/6087 [Gene]SCO1390/5543 NC_003888 SC1A8A.10, possible PTS system sugar phosphotransferase component IIA, len: 176a; similar to many e.g. TR:BAA82669 (EMBL:AB030569) putative phosphoenolpyruvate:carbohydrate phosphotransferase system family from Streptomyces griseus (149 aa) fasta scores; opt: 897, z-score: 951.7, E(): 0, 88.6% identity in 149 aa overlap and SW:P42015 (PTGA_BACST) PTS system, glucose-specific IIABC component from Bacillus stearothermophilus (324 aa) fasta scores; opt: 359, z-score: 386.4, E(): 4.3e-14, 40.6% identity in 143 aa overlap. Contains Pfam match to entry PF00358 PTS_EIIA_1, phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 4 2 18 [Reporter]SCO5874 (4E21)_PCR/6086 [Gene]SCO5874/5542 NC_003888 SC2E9.15, putative membrane protein, len: 272 aa; similar to Mycobacterium tuberculosis hypothetical protein TR:O07195 (EMBL:Z96072) MTCY05A6.14C (223 aa), fasta scores; opt: 212 z-score: 285.5 E(): 1.1e-08, 23.5% identity in 247 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 9 5 [Reporter]SCO0868 (17P15)_PCR/3708 [Gene]SCO0868/3369 NC_003888 SCM1.01c, possible regulatory protein, len: 147 aa. Similar to Streptomyces fradiae TR:Q9Z9I5 (EMBL:AB024056) regulatory protein (139 aa), fasta scores opt: 166 z-score: 217.5 E(): 9.1e-05 30.9% identity in 123 aa overlap and Streptomyces coelicolor A3(2) TR:CAB55834 (EMBL:AJ249581) putative regulator of sig15 (170 aa), fasta scores opt: 157 z-score: 205.1 E(): 0.00044 32.8% identity in 119 aa overlap. 1 4 2 17 [Reporter]SCO7115 (5A21)_PCR/6085 [Gene]SCO7115/5541 NC_003888 SC4B10.16c, conserved hypothetical protein, len: 366 aa; similar to TR:Q9ZN75 (EMBL:AB022095) Streptomyces griseus ORF2 protein, 352 aa; fasta scores: opt: 941 z-score: 1090.4 E(): 0; 44.1% identity in 356 aa overlap. Contains match to Prosite entry PS00455 Putative AMP-binding domain signature 4 2 9 4 [Reporter]SCO1659 (18L15)_PCR/3707 [Gene]SCO1659/3368 NC_003888 SCI52.01, glpF, probable glycerol uptake facilitator protein, len: 264 aa; N-terminal region identical to previously sequenced SW:GLPF_STRCO (EMBL:X14188) Streptomyces coelicolor probable glycerol uptake facilitator protein (fragment) GlpF or GylA, 80 aa, and similar to SW:GLPF_BACSU (EMBL:M99611) Bacillus subtilis glycerol uptake facilitator protein GlpF, 274 aa; fasta scores: opt: 520 Z-score: 611.2 bits: 120.8 E(): 1.8e-26; 51.163% identity in 258 aa overlap. Contains Pfam match to entry PF00230 MIP, Major intrinsic protein and match to Prosite entry PS00221 MIP family signature. Also contains possible hydrophobic membrane spanning regions 1 4 2 16 [Reporter]SCO2171 (6M17)_PCR/6084 [Gene]SCO2171/5540 NC_003888 SC5F7.30, probable secreted protease, len: 362aa; similar to many egs. SW:HTRA_ECOLI Protease Do from Escherichia coli (474 aa) fasta scores; opt: 505, z-score: 503.1, E(): 1.1e-20, (39.1% identity in 294 aa overlap). Contains possible membrane spanning hydrophobic regions. Also contains Pfam matches to entry PF00089 trypsin, Trypsin and to entry PF00595 PDZ, PDZ domain (Also known as DHR or GLGF). 4 2 9 3 [Reporter]SCO1677 (19H15)_PCR/3706 [Gene]SCO1677/3367 NC_003888 SCI52.19, conserved hypothetical protein, len: 124 aa; similar to TR:BAB35158 (EMBL:AP002556) Escherichia coli O157:H7 hypothetical 17.0 kDa protein ECS1735, 152 aa; fasta scores: opt: 397 Z-score: 489.0 bits: 96.3 E(): 1.2e-19; 52.033% identity in 123 aa overlap 4 2 8 22 [Reporter]SCO4369 (1P7)_PCR/3703 [Gene]SCO4369/3366 NC_003888 2SCD52.01, hypothetical protein (fragment), len: >312 aa; similar to N-terminal region of TR:CAB95880 (EMBL:AL359988) Streptomyces coelicolor hypothetical 26.1 kDa protein (fragment) SCD10.01, 261 aa; fasta scores: opt: 232 z-score: 233.3 E(): 1.7e-05; 32.0% identity in 225 aa overlap,SCD10.01, unknown (fragment), len: >261 aa. High content in glycine and aspartate amino acid residues. Contains 2x Pfam match to entry PF01839 FG-GAP, FG-GAP repeat 4 2 8 21 [Reporter]SCO0560 (2L7)_PCR/3702 [Gene]SCO0560/3365 NC_003888 SCF73.07c, cpeB, catalase/peroxidase, len: 740 aa; highly similar to TR:O87864 (EMBL:Y14317) Streptomyces reticuli catalase/peroxidase (EC 1.11.1.6) (740 aa), fasta scores; opt: 4603 z-score: 5243.8 E(): 0, 90.5% identity in 739 aa overlap, and similar to many e.g. CATA_MYCBO peroxidase/catalase (EC 1.11.1.6) (740 aa), fasta scores; opt: 3432 z-score: 3909.0 E(): 0, 66.7% identity in 744 aa overlap. Contains PS00436 Peroxidases active site signature, PS00435 Peroxidases proximal heme-ligand signature, PS00017 ATP/GTP-binding site motif A (P-loop), and Pfam match to entry PF00141 peroxidase 4 2 8 19 [Reporter]SCO6050 (4D7)_PCR/3700 [Gene]SCO6050/3364 NC_003888 SC1B5.10c, probable lipoprotein, len: 289 aa; contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 4 2 8 18 [Reporter]SCO5893 (5P3)_PCR/3699 [Gene]SCO5893/3363 NC_003888 SC3F7.13, redK, probable oxidoreductase, len: 347 aa; similar to many eukaryotic steroid dehydrogenases eg. TR:Q62878 (EMBL:L17138) 3 hydroxysteroid dehydrogenase from Rattus norvegicus (373 aa), fasta scores; opt: 103 z-score: 378.9 E(): 6.6e-14, 30.4% identity in 355 aa overlap; and to Mycobacterium tuberculosis putative dehydrogenase TR:O06373 (EMBL:Z95436) MTCY15C10.18 (314 aa), fasta scores; opt: 204 z-score: 417.9 E(): 4.4e-16, 27.7% identity in 339 aa overlap 3 1 14 7 [Reporter]SCO1584 (15K24)_PCR/1300 [Gene]SCO1584/1189 NC_003888 SCI35.06, unknown, len: 152 aa 4 2 8 17 [Reporter]SCO6666 (6L3)_PCR/3698 [Gene]SCO6666/3362 NC_003888 SC5A7.16c, probable integral membrane protein len: 705 aa; similar to many hypothetical membrane proteins e.g. M. tuberculosis TR:E1299723 (EMBL:AL021928) MMPL3 (944 aa), fasta scores; opt: 482 z-score: 907.8 E(): 0, 33.3% identity in 744 aa overlap 3 1 14 6 [Reporter]SCO3641 (16G24)_PCR/1299 [Gene]SCO3641/1188 NC_003888 SCH10.19c, probable two-component system sensor kinase, len: 459 aa; similar to many e.g. TR:Q53893 (EMBL:U51332), AbsA1, Streptomyces coelicolor histidine kinase homolog involved in negative regulation of antibiotic synthesis (571 aa), fasta scores; opt: 332 z-score: 342.7 E(): 8.9e-12, 30.6% identity in 402 aa overlap. Contains hydrophobic, probable membrane-spanning regions. Similar to many other putative two-component system sensor kinases from Streptomyces coelicolor e.g. TR:O86631 (EMBL:AL031155) SC3A7.30, probable two-component system sensor (429 aa) (36.2% identity in 409 aa overlap). Contains probable coiled-coil from 264 to 292 (29 residues) (Max score: 1.426, probability 0.81). The start codon may be further downstream, which would agree with some other bacterial two-component system sensors; however, nitrate/nitrite sensory proteins have a length comparable to this prediction 4 2 8 16 [Reporter]SCO0317 (7H3)_PCR/3697 [Gene]SCO0317/3361 NC_003888 SC5G9.26c, probable transmembrane transport protein, len: 420 aa; shows weak similarity to many efflux pumps e.g. SW:CMLR_STRLI (EMBL:X59968), cmlR, Streptomyces lividans chloramphenicol resistance protein (392 aa), fasta scores; opt: 276 z-score: 292.9 E(): 6e-09, 27.3% identity in 407 aa overlap. Contains hydrophobic, possible membrane-spanning regions 3 1 14 5 [Reporter]SCO0358 (17C24)_PCR/1298 [Gene]SCO0358/1187 NC_003888 SCF41.17, possible racemase, len: 380 aa. Duplication of SCF41.06. Similar to Pseudomonas putida SW:MANR_PSEPU (EMBL; M19043) mandelate racemase (EC 5.1.2.2) (359 aa), fasta scores opt: 374 z-score: 431.2 E(): 1.2e-16 29.8% identity in 362 aa overlap. Contains a Pfam match to entry PF01188 MR_MLE, Mandelate racemase / muconate lactonizing enzyme family. This gene is duplicated on this cosmid and shares 74.3% identity, in 378 aa overlap, with the upstream gene, SCF41.06 4 2 8 15 [Reporter]SCO2159 (8D3)_PCR/3696 [Gene]SCO2159/3360 NC_003888 SC6G10.32, unknown, len: 69aa; 3 1 14 4 [Reporter]SCP1.173 (18O20)_PCR/1297 [Gene]SCP1.173/1186 NC_003888 SCP1.173, possible membrane protein, len: 139aa; contains a possible membrane-spanning hydrophobic region. 3 1 14 3 [Reporter]SCO4057 (19K20)_PCR/1296 [Gene]SCO4057/1185 NC_003888 2SCD60.23c, probable transport integral membrane protein, len: 296 aa; similar to SW:AQP3_HUMAN (EMBL:AB001325) Homo sapiens aquaporin 3 Aqp3, 292 aa; fasta scores: opt: 571 z-score: 644.7 E(): 2.4e-28; 40.4% identity in 275 aa overlap. Contains Pfam match to entry PF00230 MIP, Major intrinsic protein and match to Prosite entry PS00221 MIP family signature 3 1 14 2 [Reporter]SCO7483 (20C20)_PCR/1295 [Gene]SCO7483/1184 NC_003888 SCBAC17A6.16, conserved hypothetical protein, len: 135aa: similar to many eg. TR:BAB48518 (EMBL:AP002996) hypothetical protein MLL1058 from Rhizobium loti (148 aa) fasta scores; opt: 285, Z-score: 348.9, 39.850% identity (41.732% ungapped) in 133 aa overlap. Contains Pfam match to entry PF01042 UPF0076, YjgF family. 3 1 13 22 [Reporter]SCO1010 (1C16)_PCR/1293 [Gene]SCO1010/1183 NC_003888 2SCG2.23, possible integral membrane transport protein, len: 478 aa; highly similar to TR:O68895 (EMBL:AF056309) Streptomyces argillaceus membrane protein ORFA, 414 aa; fasta scores: opt: 1923 z-score: 2121.0 E(): 0; 82.0% identity in 362 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and match to Prosite entry PS00217 Sugar transport proteins signature 2. Also contains possible hydrophobic membrane spanning regions 3 1 13 21 [Reporter]SCO4824 (2O12)_PCR/1292 [Gene]SCO4824/1182 NC_003888 SC2A6.09, folD, bifunctional protein (methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase), len: 284 aa; highly similar to SW:FOLD_ECOLI (EMBL:M74789) Escherichia coli FolD, bifunctional protein (methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)), 287 aa; fasta scores: opt: 784 z-score: 905.2 E(): 0; 44.2% identity in 278 aa overlap. Contains Pfam match to entry PF00763 THF_DHG_CYH, Tetrahydrofolate dehydrogenase/cyclohydrolase 3 1 13 20 [Reporter]SCO5925 (3K12)_PCR/1291 [Gene]SCO5925/1181 NC_003888 SC10A5.30c, unknown, len: 83 aa 3 1 13 19 [Reporter]SCO6029 (4G12)_PCR/1290 [Gene]SCO6029/1180 NC_003888 SC1C3.17c, probable two-component regulator, len: 220 aa; similar to many e.g. DEGU_BACSU transcriptional regulatory protein degU (229 aa), fasta scores; opt: 168 z-score: 264.1 E(): 1.8e-07, 27.4% identity in 223 aa overlap. PS00622 Bacterial regulatory proteins, luxR family signature. Contains Pfam match to entry GerE PF00196, Bacterial regulatory proteins, luxR family, score 83.75 and to entry response_reg PF00072, Response regulator receiver domain, score 36.16 1 4 2 15 [Reporter]SCO2119 (7I17)_PCR/6083 [Gene]SCO2119/5539 NC_003888 SC6E10.13c, pfkA, 6-phosphofructokinase, len: 342 aa; previously sequenced as TR:O08333 (EMBL:U51728) Streptomyces coelicolor phosphofructokinase ATP-PFK (342 aa). There are some differences in the flanking nucleotides between this and the previous sequence (EMBL:U51728), but the proteins remain 100% identical. Contains Pfam match to entry PF00365 PFK, Phosphofructokinase and S00433 Phosphofructokinase signature 1 4 2 14 [Reporter]SCO6856 (8E17)_PCR/6082 [Gene]SCO6856/5538 NC_003888 SC7F9.08c, unknown, len: 215 aa. Weakly similar to Streptomyces coelicolor TR:Q9X899 (EMBL:AL049645) hypothetical 25.5 KD protein, SCE2.04 (241 aa), fasta scores opt: 158 z-score: 178.7 E():0.015 28.0% identity in 150 aa overlap. 1 4 2 13 [Reporter]SCO7547 (9A17)_PCR/6081 [Gene]SCO7547/5537 NC_003888 SC5F1.01, possible sulfatase (fragment), len: >136 aa; low similarity to SW:YIDJ_ECOLI (EMBL:L10328) Escherichia coli hypothetical 57.3 kDa protein in EmrD-GlvG intergenic region putative sulfatase YdjI (EC 3.1.6.-), 497 aa; fasta scores: opt: 147 z-score: 185.4 E(): 0.0085; 29.6% identity in 115 aa overlap,SC8G12.23, possible sulphatase, partial CDS, len: >430 aa. Similar to many Eukaryotic sulphatases e.g. Homo sapiens (Human) SW:IDS_HUMAN(EMBL:M58342) iduronate 2-sulfatase precursor (EC 3.1.6.13) (550 aa), fasta scores opt: 326 z-score: 382.2 E(): 7.9e-14 28.4% identity in 423 aa overlap. Also similar to Escherichia coli TR:YIDJ_ECOLI(EMBL:L10328) hypothetical 57.3 kd protein (497 aa), fasta scores opt: 221 z-score:0.0 E(): 0.0 31.23% identity in 397 aa overlap. Contains a Prosite hit to PS00523 Sulfatases signature 1 and a Pfam match to entry PF00884 Sulfatase, Sulfatase. 1 4 2 12 [Reporter]SCO2321 (10M13)_PCR/6080 [Gene]SCO2321/5536 NC_003888 SCC53.12, possible secreted protein, len: 419aa; contains possible cleavable N-terminal signal sequence and Pfam match to entry PF00432 prenyltrans, Prenyltransferase and squalene oxidase repeat. 1 4 2 11 [Reporter]SCO4692 (11I13)_PCR/6079 [Gene]SCO4692/5535 NC_003888 SCD31.17, hypothetical protein, len: 215 aa; similar to TR:Q9F3G1 (EMBL:AL392175) Streptomyces coelicolor hypothetical protein 2SCD4.10c, 201 aa; fasta scores: opt: 776 Z-score: 867.6 E(): 1.1e-40; 59.000% identity in 200 aa overlap 1 4 2 10 [Reporter]SCO4235 (12E13)_PCR/6078 [Gene]SCO4235/5534 NC_003888 SCD8A.08, probable cysteinyl-tRNA synthetase, len: 613 aa; C-terminal region highly similar to SW:SYC_ECOLI (EMBL:X56234) Escherichia coli cysteinyl-tRNA synthetase (EC 6.1.1.16) CysS, 461 aa; fasta scores: opt: 1267 z-score: 1248.2 E(): 0; 43.7% identity in 469 aa overlap. Contains Pfam match to entry PF01406 tRNA-synt_1e, tRNA synthetases class I (C) and a N-terminal region degenerate 6x repeat 1 4 2 9 [Reporter]SCO0706 (13A13)_PCR/6077 [Gene]SCO0706/5533 NC_003888 SCF42.16c, possible lipoprotein, len: 427 aa, Weakly similar to Pseudomonas aeruginosa SW:BRAC_PSEAE(EMBL:M31071) leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein precursor (373 aa), fasta scores opt: 239 z-score: 250.6 E(): 1.4e-06 24.0% identity in 341 aa overlap. Contains an appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. 1 4 2 8 [Reporter]SCO2509 (14M9)_PCR/6076 [Gene]SCO2509/5532 NC_003888 SCC121.12c, uppS, undecaprenyl phosphate synthetase, len: 277 aa; similar to SW:UPPS_MYCTU (EMBL:Z95208) Mycobacterium tuberculosis undecaprenyl pyrophosphate synthetase (EC 2.5.1.31) (Upp synthetase) UppS, 296 aa; fasta scores: opt: 1144 z-score: 1315.8 E(): 0; 63.6% identity in 247 aa overlap and to SW:UPPS_ECOLI (EMBL:D83536) Escherichia coli undecaprenyl pyrophosphate synthetase (EC 2.5.1.31) (Upp synthetase) UppS, 253 aa; fasta scores: opt: 642 z-score: 743.0 E(): 0; 43.1% identity in 232 aa overlap. Contains Pfam match to entry PF01255 UPF0015 4 2 8 14 [Reporter]SCO6583 (8P23)_PCR/3695 [Gene]SCO6583/3359 NC_003888 SC8A6.04c, probable transferase, len: 410 aa; highly similar to TR:O06644 (EMBL:U82167) formyl-CoA transferase from Oxalobacter formigenes (428 aa), fasta scores; opt: 95 4 z-score: 1732.0 E(): 0, 50.7% identity in 428 aa overlap. Also similar to many dehydratases e.g. CAIB_ECOLI L-carnit ine dehydratase (EC 4.2.1.89) (405 aa), fasta scores; opt: 285 z-score: 317.7 E(): 1.9e-10, 26.2% identity in 427 aa o verlap 1 4 2 7 [Reporter]SCO0203 (15I9)_PCR/6075 [Gene]SCO0203/5531 NC_003888 SCJ12.15c, probable two-component sensor. len: 585 aa. Similarity to Streptomyces coelicolor TR:Q9ZBY4 (EMBL; AL034355) putative two component sensor SCD78.15 (560 aa), fasta scores opt: 1439 z-score: 1571.0 E(): 0 44.7% identity in 553 aa overlap. Also some similarity to Pseudomonas aeruginosa TR:O54040 (EMBL; Y15252) nitrate/nitrite sensory protein NarX (622 aa), fasta scores opt: 315 z-score: 344.5 E(): 7.9e-1 29.0% identity in 297 aa overlap. Contains 2x Pfam match to entry PF01590 GAF and potential membrane spanning hydrophobic domains. Probable coiled-coil from 17 to 48 (32 residues). 4 2 8 13 [Reporter]SCO1507 (9L23)_PCR/3694 [Gene]SCO1507/3358 NC_003888 SC9C5.31c, possible integral membrane protein, len: 240 aa; similar to TR:O69464 (EMBL:AL023635) Mycobacterium leprae putative integral membrane protein MLCB1243.07, 214 aa; fasta scores: opt: 511 z-score: 600.4 E(): 5.7e-26; 36.1% identity in 191 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 2 6 [Reporter]SCO2898 (16E9)_PCR/6074 [Gene]SCO2898/5530 NC_003888 SCE6.35c, sugE, molecular chaperone, len: 106 aa. Highly similar to several proposed molecular chaperones e.g. Escherichia coli SW:SUGE_ECOLI(EMBL:X69949) SugE (105 aa), fasta scores opt: 394 z-score: 454.4 E(): 7e-18 51.0% identity in 104 aa overlap. Contains a Pfam match to entry PF00893 DUF7, Integral membrane protein and multiple possible membrane spanning hydrophobic domains. 4 2 8 12 [Reporter]SCO2728 (10H23)_PCR/3693 [Gene]SCO2728/3357 NC_003888 SCC46.13, unknown, len: 132 aa 4 2 8 11 [Reporter]SCO4097 (11D23)_PCR/3692 [Gene]SCO4097/3356 NC_003888 SCD17.01, possible amino acid/metabolite permease, partial CDS, len: >389 aa. Similar to many Eukaryotic permeases e.g. Arabidopsis thaliana TR: O22509 (EMBL; AF019637) putative amino acid or GABA permease (516 aa), fasta scores opt: 827 z-score: 942.8 E(): 0 41.8% identity in 385 aa overlap as well as Escherichia coli SW:YEEF_ECOLI (EMBL; U00009) hypothetical 49.8 KD transport protein in sbcB-hisL intergenic region (454 aa), fasta scores opt: 256 z-score: 295.7 E(): 4.1e-09 24.6% identity in 293 aa overlap. Contains a Pfam match to entry PF00324 aa_permeases, Amino acid permease and multiple possible membrane spanning hydrophobic domains. The 5' region of this CDS is located on cosmid SCD25.,SCD25.33, possible integral membrane protein, partial CDS, len: 156 aa. High level of similarity with Eukaryotic permeases e.g. Arabidopsis thaliana TR:O22509 (EMBL; AF019637) putative amino acid or GABA permease (516 aa), fasta scores opt: 470 z-score: 557.7 E(): 1e-23 53.1% identity in 128 aa overlap and Bacillus subtilis TR:O07576 (EMBL; Y14082) hypothetical 49.7 KD protein (456 aa), fasta scores opt: 128 z-score: 158.4 E(): 0.18 23.3% identity in 133 aa overlap. Contains possible membrane spanning hydrophobic domains. 4 2 8 10 [Reporter]SCO2933 (12P19)_PCR/3691 [Gene]SCO2933/3355 NC_003888 SCE19A.33, probable permease binding-protein component, len: 322 aa; similar to SW:PROX_BACSU (EMBL:U38418), proX, Bacillus subtilis glycine betaine-binding protein precursor (313 aa), fasta scores; opt: 300 z-score: 341.4 E(): 1.1e-11, 27.5% identity in 320 aa overlap and to similar putative permease components. Similar to SC9B1.12 (EMBL:AL049727) S.coelicolor probable secreted substrate-binding protein (320 aa) (30.4% identity in 329 aa overlap). Contains a probable N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. May be part of a multicomponent transporter which includes SCE19A.30, SCE19A.31, SCE19A.32 and SCE19A.33 4 2 8 9 [Reporter]SCO0403 (13L19)_PCR/3690 [Gene]SCO0403/3354 NC_003888 SCF51.02c, hypothetical protein, len: 187 aa; similar to various hypothetical proteins, e.g. TR:CAB56690 (EMBL:AL121596) Streptomyces coelicolor SCF51A.38 hypothetical 18.5 KD protein, 169 aa; fasta scores: opt: 176 z-score: 225.6 E(): 3.4e-05; 32.9% identity in 173 aa overlap 4 2 8 8 [Reporter]SCO1173 (14H19)_PCR/3689 [Gene]SCO1173/3353 NC_003888 SCG11A.04, possible transcriptional regulator, len: aa; similar to TR:O31551 (EMBL:Z99108) Bacillus subtilis transcriptional regulator AcoR, 605 aa; fasta scores: opt: 393 z-score: 446.5 E(): 1.7e-17; 34.7% identity in 199 aa overlap 3 1 13 18 [Reporter]SCO5460 (5C12)_PCR/1289 [Gene]SCO5460/1179 NC_003888 SC3D11.17, possible AbaA-like regulatory protein, len: 220 aa; similar to many from Streptomyces spp. e.g. TRNEW:AAD46514 (EMBL:AF145724) putative regulatory protein from Streptomyces albus (137 aa) fasta scores; opt: 258, z-score: 275.0, E(): 6.6e-08, 45.3% identity in 128 aa overlap and TR:Q53897 (EMBL:X60316) AbaA from Streptomyces coelicolor (192 aa) fasta scores; opt: 226, z-score: 240.9, E(): 5.3e-06, 32.3% identity in 189 aa overlap. 4 2 8 6 [Reporter]SCO3478 (16P15)_PCR/3687 [Gene]SCO3478/3352 NC_003888 SCE65.14c, probable dehydrogenase, len: 344 aa; similar to TR:O50095 (EMBL:AP000006) Pyrococcus horikoshii 307aa long hypothetical phosphoglycerate dehydrogenase PH1387, 307 aa; fasta scores: opt: 687 z-score: 777.1 E(): 0; 40.5% identity in 291 aa overlap and to SW:SERA_METTH (EMBL:AE000870;) Methanobacterium thermoautotrophicum D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) SerA, 525 aa; fasta scores: opt: 664 z-score: 748.1 E(): 0; 39.0% identity in 282 aa overlap. Contains Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases and match to Prosite entry PS00670 D-isomer specific 2-hydroxyacid dehydrogenases signature 2 3 1 13 17 [Reporter]SCO7452 (6O8)_PCR/1288 [Gene]SCO7452/1178 NC_003888 SC5C11.09c, possible O-methyltransferase, len: 342 aa. Similar to many e.g. Streptomyces glaucescens SW:TCMN_STRGA (EMBL:M80674) multi-functional cyclase-dehydratase-3-O-methyl transferase, TcmN, forming the first step in the biosynthesis of the polyketide antibiotic tetracenomycin C (494 aa), fasta scores opt: 472 z-score: 554.8 E(): 1.8e-23 30.8% identity in 308 aa overlap and Streptomyces lavendulae TR:Q9X5T6(EMBL:AF127374) O-methyltransferase, MmcR, found within the mitomycin C biosynthetic cluster (351 aa), fasta scores opt: 654 z-score: 769.9 E(): 0 37.1% identity in 340 aa overlap. Contains a Pfam match to entry PF00891 Methyltransf_2, O-methyltransferase. 4 2 8 5 [Reporter]SCO3585 (17L15)_PCR/3686 [Gene]SCO3585/3351 NC_003888 SCH66.06, unknown, len: 113aa; 3 1 13 15 [Reporter]SCO5324 (8G8)_PCR/1286 [Gene]SCO5324/1177 NC_003888 SC6G9.09c, possible oxidoreductase, len: 226 aa; weak similarity to SW:NOX_THETH (EMBL:X60110) Thermus aquaticus NADH dehydrogenase (205 aa), fasta scores; opt: 159 z-score: 201.0 E(): 0.00073, 26.7% identity in 191 aa overlap. Similar to TR:O75989 (EMBL:AL031010) Homo sapiens hypothetical protein (184 aa) (40.0% identity in 185 aa overlap) and to part of SW:YKH5_CAEEL (EMBL:L23649) Caenorhabditis elegans hypothetical protein (359 aa) (28.6% identity in 185 aa overlap). Weak similarity to SW:NOX_THETH (EMBL:X60110) Thermus aquaticus NADH dehydrogenase (205 aa) (26.7% identity in 191 aa overlap). Contains Pfam match to entry PF00881 Nitroreductase, Nitroreductase family 4 2 8 4 [Reporter]SCO1917 (18H15)_PCR/3685 [Gene]SCO1917/3350 NC_003888 SCI7.35c, unknown, len: 197aa; similar to many eg. TR:P95092 (EMBL:Z83866) hypothetical protein from Mycobacterium tuberculosis (202 aa) fasta scores; opt: 398, z-score: 482.3, E(): 1.6e-19, (40.3% identity in 191 aa overlap). 3 1 13 14 [Reporter]SCO7750 (9C8)_PCR/1285 [Gene]SCO7750/1176 NC_003888 SC8D11.41, hypothetical protein, len: 137 aa; low similarity to TR:Q9RIS6 (EMBL:AJ249581) Streptomyces coelicolor putative anti anti sigma factor SCE25.08c, 125 aa; fasta scores: opt: 110 z-score: 148.5 E(): 0.98; 33.8% identity in 77 aa overlap 3 1 13 13 [Reporter]SCO2783 (10O4)_PCR/1284 [Gene]SCO2783/1175 NC_003888 SCC105.14, probable monooxygenase, len: 413 aa; similar to SW:RHSE_RHIME (EMBL:AF110737) Rhizobium meliloti rhizobactin siderophore biosynthesis protein RhsE (EC 1.-.-.-), 454 aa; fasta scores: opt: 616 z-score: 727.2 E(): 0; 42.3% identity in 430 aa overlap and to SW:IUCD_ECOLI (EMBL:M18968) Escherichia coli L-lysine 6-monooxygenase (EC 1.13.12.10) IucD, 425 aa; fasta scores: opt: 802 z-score: 946.5 E(): 0; 31.6% identity in 414 aa overlap 3 1 13 12 [Reporter]SCO5278 (11K4)_PCR/1283 [Gene]SCO5278/1174 NC_003888 SCCB12.02, possible ion chelatase, len: 655 aa; similar to TR: SW:CHLD_SYNP7 (EMBL:AB003135) Synechococcus sp. magnesium-chelatase subunit ChlD (MG-protoporphyrin IX chelatase) ChlD, 677 aa; fasta scores: opt: 304 z-score: 269.4 E(): 1.5e-07; 26.6% identity in 688 aa overlap 3 1 13 11 [Reporter]SCO4790 (12G4)_PCR/1282 [Gene]SCO4790/1173 NC_003888 SCD63A.01c, possible integral membrane protein (fragment), len: >35 aa,SCD63.22c, possible integral membrane protein (fragment), len: 171 aa. Contains possible hydrophobic membrane spanning regions 3 1 13 10 [Reporter]SCO3198 (13C4)_PCR/1281 [Gene]SCO3198/1172 NC_003888 SCE22.15c, probable deoR-family transcriptional regulator, len: 253 aa; similar to TR:Q9X9X5 (EMBL:AL096743) Streptomyces coelicolor putative transcriptional regulator SCI7.15, 258 aa; fasta scores: opt: 609 z-score: 704.8 E(): 8.5e-32; 43.1% identity in 248 aa overlap and to SW:GATR_ECOLI (EMBL:AE000298) Escherichia coli galactitol utilization operon repressor GatR, 259 aa; fasta scores: opt: 438 z-score: 509.7 E(): 6.3e-21; 33.6% identity in 256 aa overlap. Contains Pfam match to entry PF00455 deoR, Bacterial regulatory proteins, deoR family 3 1 13 9 [Reporter]SCO5029 (13O24)_PCR/1280 [Gene]SCO5029/1171 NC_003888 SCK7.02, possible secreted protein, len: 238 aa; similar to C-terminal region of TR:CAB93446 (EMBL:AL357591) Streptomyces coelicolor hypothetical 16.0 kDa protein SCC53.17, 154 aa; fasta scores: opt: 477 z-score: 484.5 E(): 1.6e-19; 64.4% identity in 101 aa overlap. Contains possible N-terminal region signal peptide sequence and possible coiled-coil region at aprox. residues 82..123 3 1 13 8 [Reporter]SCO2962 (14K24)_PCR/1279 [Gene]SCO2962/1170 NC_003888 SCE59.21c, possible bi-functional transferase/deacetylase, len: 734 aa; similar to TR:Q9XAP6 (EMBL:AL078618) Streptomyces coelicolor putative bi-functional transferase/deacetylase SC10A7.29c, 743 aa; fasta scores: opt: 2045 z-score: 2233.4 E(): 0; 59.5% identity in 726 aa overlap and N-terminal region similar to SW:NODB_RHIME (EMBL:X01649) Rhizobium meliloti chitooligosaccharide deacetylase (EC 3.5.1.-) (nodulation protein B) NodB, 217 aa; fasta scores: opt: 411 z-score: 455.6 E(): 5.8e-18; 37.9% identity in 198 aa overlap. Contains Pfam match to entry PF01522 Polysac_deacet, Polysaccharide deacetylase at N-terminal region and Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases at C-terminal region and possible hydrophobic membrane spanning regions 1 4 2 5 [Reporter]SCO5151 (17A9)_PCR/6073 [Gene]SCO5151/5529 NC_003888 SCP8.14, unknown, len: 248 aa 1 4 2 4 [Reporter]SCO1153 (18M5)_PCR/6072 [Gene]SCO1153/5528 NC_003888 SCG8A.07, tesB, acyl-CoA thioesterase II, len: 288 aa; similar to many e.g. SW:P23911 (TESB_ECOLI) TesB, acyl-CoA thioesterase II from Escherichia coli (285 aa) fasta scores; opt: 758, z-score: 852.2, E(): 0, 44.1% identity in 286 aa overlap and SCC105.04c from Streptomyces coelicolor (290 aa) fasta scores; opt: 1004, z-score: 1023.6, E(): 0, 57.0% identity in 291 aa overlap. 1 4 2 3 [Reporter]SCP1.290c (19I5)_PCR/6071 [Gene]SCP1.290c/5527 NC_003888 SCP1.290c, possible ATP/GTP-binding protein, len: 602aa; weakly similar to others from plasmids eg. TR:Q9XAX4 (EMBL:U88088) hypothetical protein from plasmid pRA2 of Pseudomonas alcaligenes (522 aa) fasta scores; opt: 263, z-score: 287.6, E(): 1.5e-08, 22.9% identity in 498 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 4 2 2 [Reporter]SCP1.165 (20A5)_PCR/6070 [Gene]SCP1.165/5526 NC_003888 SCP1.165, possible secreted protein/lipoprotein, len: 200aa; contains a possible cleavable N-terminal signal sequence and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 1 4 1 22 [Reporter]SCO5177 (1A1)_PCR/6068 [Gene]SCO5177/5525 NC_003888 2SC3B6.01, unknown, len: 249 aa 1 4 1 21 [Reporter]SCO1755 (1M21)_PCR/6067 [Gene]SCO1755/5524 NC_003888 2SCI34.08c, unknown, len: 282 aa; similar to TR:CAC47960 (EMBL:AJ320278) Streptomyces avermitilis hypothetical protein, 278 aa; fasta scores: opt: 962 Z-score: 1035.8 E():4.6e-50; 63.441% identity in 279 aa overlap 1 4 1 20 [Reporter]SCO2758 (2I21)_PCR/6066 [Gene]SCO2758/5523 NC_003888 SCC57A.29, nagA, beta-N-acetylglucosaminidase (putative secreted protein), len: 615 aa, Highly similar to Streptomyces thermoviolaceus TR:O82840 (EMBL:AB008771) beta-N-acetylglucosaminidase precursor (EC 3.2.1.30) (632 aa), fasta scores opt: 3169 z-score: 3414.1 E():0 80.0% identity in 614 aa overlap. Note the N-terminus of the S. coelicolor protein varies significantly from that of the S. thermoviolaceus protein. Contains a Prosite hit to PS00775 Glycosyl hydrolases family 3 active site and a Pfam match to entry PF00933 Glyco_hydro_3, Glycosyl hydrolase family 3. Also contains a possible N-terminal region signal peptide sequence 1 4 1 19 [Reporter]SCO7383 (3E21)_PCR/6065 [Gene]SCO7383/5522 NC_003888 SC10G8.10, doubtful CDS, len: 126 aa. 4 2 8 3 [Reporter]SCO1674 (19D15)_PCR/3684 [Gene]SCO1674/3349 NC_003888 SCI52.16c, possible secreted protein, len: 259 aa; similar to TR:Q9X7U2 (EMBL:AL049863) Streptomyces coelicolor hypothetical 22.7 kDa protein SC5H1.35c, 237 aa; fasta scores: opt: 566 Z-score: 369.4 bits: 75.8 E(): 5.3e-13; 46.154% identity in 260 aa overlap. High content in glycine amino acid residues. Also contains the following repeats: 2x(SPGV-SGN-V--P--VPVNVCGN--DVVG-LNP--GN-C-N-GGG) and 4x(GG-) and a possible N-terminal region signal peptide sequence 1 4 1 18 [Reporter]SCO5867 (4A21)_PCR/6064 [Gene]SCO5867/5521 NC_003888 SC2E9.08, unknown, len: 194 aa 4 2 8 2 [Reporter]SCO5075 (20L11)_PCR/3683 [Gene]SCO5075/3348 NC_003888 SCBAC28G1.01, possible oxidoreductase, previously sequenced as ORF4, len: 167 aa; identical to C-terminal region of previously sequenced TR:Q53929 (EMBL:X62373) Streptomyces coelicolor ORF4, 306 aa; fasta scores: opt: 1029 Z-score: 1146.7 bits: 219.4 E(): 2.9e-56; 95.808% identity in 167 aa overlap. Contains match to Prosite entry PS01162 Quinone oxidoreductase / zeta-crystallin signature,SCBAC20F6.18, ORF4, possible oxidoreductase, len: 170 aa; identical to N-terminal region of the previously sequenced TR:Q53929 (EMBL:X62373) Streptomyces coelicolor ORF 4, 306 aa. Contains match to Prosite entry PS01162 Quinone oxidoreductase / zeta-crystallin signature 1 4 1 17 [Reporter]SCO6699 (5M17)_PCR/6063 [Gene]SCO6699/5520 NC_003888 SC4C6.09c, pcaG, protocatechuate 3,4-dioxygenase alpha subunit, len: 201 aa; highly similar to pcaG (EMBL:AF109386) Streptomyces sp. 2065 protocatechuate 3,4-dioxygenase alpha subunit (201 aa), fasta scores; opt: 1144 z-score: 1272.2 E(): 0, 80.1% identity in 201 aa overlap 4 2 7 22 [Reporter]SCO4224 (1L7)_PCR/3681 [Gene]SCO4224/3347 NC_003888 2SCD46.38c, unknown, len: 421aa; 4 2 7 21 [Reporter]SCO4744 (2H7)_PCR/3680 [Gene]SCO4744/3346 NC_003888 SC6G4.22c, acpS, probable holo-[acyl-carrier protein ] synthase, len: 123 aa; similar to many e.g. ACPS_ECOLI holo-[acyl-carrier protein] synthase (EC 2.7.8.7) (125 aa), fasta scores; opt: 237 z-score: 303.8 E(): 1.1e-09, 38.7% i dentity in 124 aa overlap 4 2 7 19 [Reporter]SCO6513 (4P3)_PCR/3678 [Gene]SCO6513/3345 NC_003888 SC1E6.22c, unknown, len: 373 aa; similar to a hypothetical protein from M. tuberculosis TR:O06183 (EMBL:Z95387) MTCY1A10.03C (374 aa), fasta scores; opt: 425 z-score: 395.8 E(): 9.2e-15, 30.2% identity in 371 aa overlap 4 2 7 18 [Reporter]SCO5888 (5L3)_PCR/3677 [Gene]SCO5888/3344 NC_003888 SC3F7.08, redP, probable 3-oxoacyl-[acyl-carrier-protein] synthase III, len: 335 aa; similar to many eg. FABH_ECOLI P24249 3-oxoacyl-[acyl-carrier-protein] synthase III (EC 2.3.1.41) (beta-ketoacyl-ACP synthase III) (317 aa), fasta scores; opt: 502 z-score: 881.2 E(): 0, 40.4% identity in 319 aa overlap 4 2 7 17 [Reporter]SCO6641 (6H3)_PCR/3676 [Gene]SCO6641/3343 NC_003888 SC4G2.15c, unknown, len: 404 aa; N-terminus is similar to extreme C-terminus of TR:O26587 (EMBL:AE000832) DNA helicase related protein MTH487 (Methanobacterium thermoautotrophicum) (1157 aa), fasta scores; opt: 467 z-score: 553.7 E(): 1.3e-23, 43.7% identity in 174 aa overlap 3 1 13 7 [Reporter]SCO3164 (15G24)_PCR/1278 [Gene]SCO3164/1169 NC_003888 SCE87.15c, possible integral membrane protein, len: 281 aa. Weakly similar to Bacillus subtilis SW:YWFM_BACSU (EMBL:X73124) hypothetical 31.3 KD protein in PTA 3'region (296 aa), fasta scores opt: 157 z-score: 184.7 E(): 0.0065 22.9% identity in 279 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence. Also similar to TR:CAC45825 (EMBL:AL591786) Rhizobium meliloti (Sinorhizobium meliloti) hypothetical transmembrane protein SMC01807, 278 aa; fasta scores: opt: 605 Z-score: 660.4 E(): 3.7e-29; 38.811% identity in 286 aa overlap 4 2 7 16 [Reporter]SCO0313 (7D3)_PCR/3675 [Gene]SCO0313/3342 NC_003888 SC5G9.22, probable transmembrane transport protein, len: 449 aa; similar to many transmembrane transporters e.g. SW:DGOT_ECOLI (EMBL:L10328), dgoT, Escherichia coli D-galactonate transporter (445 aa), fasta scores; opt: 332 z-score: 376.2 E(): 1.4e-13, 23.7% identity in 438 aa overlap. Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter 3 1 13 6 [Reporter]SCO0497 (16C24)_PCR/1277 [Gene]SCO0497/1168 NC_003888 SCF34.16c, probable iron-siderophore permease transmembrane protein, len: 358 aa; similar to SW:FEPD_ECOLI (EMBL:X57471), FepD, Escherichia coli ferric enterobactin transport protein (334 aa), fasta scores; opt: 796 z-score: 833.5 E(): 0, 43.9% identity in 330 aa overlap and to many other iron-siderophore permeases. Similar to others from S.coelicolor e.g. SCI51.27c (EMBL:AL109848) S.coelicolor probable iron-siderophore uptake system transmembrane component (348 aa) (46.8% identity in 344 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF01032 FecCD_family, FecCD transport family 4 2 7 15 [Reporter]SCO2131 (7P23)_PCR/3674 [Gene]SCO2131/3341 NC_003888 SC6G10.04, probable long chain fatty acid CoA ligase, len: 598 aa; similar to many e.g. TR:E1359128 (EMBL:AL034443) putative long chain fatty acid CoA ligase from Streptomyces coelicolor (608 aa) fasta scores; opt: 1347, z-score: 1506.7, E(): 0, (51.3% identity in 608 aa overlap) and SW:LCFB_RAT long chain fatty acid CoA ligase from Rattus norvegicus (Rat) (699 aa) fasta scores; opt: 568, z-score: 634.7, E(): 4.7e-28, (30.2% identity in 589 aa overlap). Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and Prosite match to PS00455 Putative AMP-binding domain signature. 3 1 13 5 [Reporter]SCO0047 (17O20)_PCR/1276 [Gene]SCO0047/1167 NC_003888 SCJ4.28c, possible transcriptional regulator, len: 384 aa; C-terminal similar to many transcriptional regulator eg. SW:SLYA_ECOLI transcriptional regulator from Escherichia coli (146 aa) fasta scores; opt: 156, z-score: 184.9, E(): 0.0061, (24.6% identity in 122 aa overlap). Contains Pfam match to entry PF01047 MarR, MarR family 4 2 7 14 [Reporter]SCO2418 (8L23)_PCR/3673 [Gene]SCO2418/3340 NC_003888 SC8A2.06c, possible secreted protein, len: 354 aa; similar to TR:O53968 (EMBL:AL022073) Mycobacterium tuberculosis hypothetical 36.5 kDa protein MTV051.05, 342 aa; fasta scores: opt: 665 z-score: 735.0 E(): 0; 37.6% identity in 343 aa overlap. Contains Pfam match to entry PF02470 mce. Also contains possible N-terminal region signal peptide sequence 3 1 13 4 [Reporter]SCP1.262 (18K20)_PCR/1275 [Gene]SCP1.262/1166 NC_003888 SCP1.262, unknown, len: 80aa; 3 1 13 3 [Reporter]SCP1.154 (19G20)_PCR/1274 [Gene]SCP1.154/1165 NC_003888 SCP1.154, possible ATP/GTP-binding protein, len: 726aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 1 13 2 [Reporter]SCO5063 (20O16)_PCR/1273 [Gene]SCO5063/1164 NC_003888 SCBAC20F6.06c, hypothetical protein, len: 83 aa; similar to TR:Q9XAG7 (EMBL:AL079356) Streptomyces coelicolor hypothetical 8.8 kDa protein SC6G9.07, 84 aa; fasta scores: opt: 167 Z-score: 224.6 bits: 45.9 E(): 6.3e-05; 43.103% identity in 58 aa overlap 3 1 12 22 [Reporter]SCO1275 (1O12)_PCR/1271 [Gene]SCO1275/1163 NC_003888 2SCG18.22, probable halogenase, len: 585 aa; similar to TR:P95482 (EMBL:U74493) Pseudomonas fluorescens halogenase PrnC, 567 aa ;fasta scores: opt: 1486 z-score: 1773.6 E(): 0; 40.9% identity in 541 aa overlap 3 1 12 21 [Reporter]SCO5135 (2K12)_PCR/1270 [Gene]SCO5135/1162 NC_003888 SC9E12.20, fdxA1, ferredoxin, len: 106 aa; similar to SW:FER_SACER (EMBL:M61119) Saccharopolyspora erythraea ferredoxin FdxA, 105 aa; fasta scores: opt: 618 z-score: 759.8 E(): 0; 78.1% identity in 105 aa overlap and to TR:CAC04216 (EMBL:AL391515) Streptomyces coelicolor FdxA, ferredoxin, 129 aa; fasta scores: opt: 520 z-score: 702.1 E(): 1.8e-33; 65.7% identity in 105 aa overlap. Contains Pfam match to entry PF00037 fer4, 4Fe-4S binding domain and match to Prosite entry PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature 3 1 12 20 [Reporter]SCO5877 (3G12)_PCR/1269 [Gene]SCO5877/1161 NC_003888 SC2E9.18, redD, transcriptional regulator, len: 350 aa; identical to REDD_STRCO P16922 redd protein from Streptomyces coelicolor (350 aa). Possible alternative start site at 15238 (aa 84) 3 1 12 19 [Reporter]SCO6025 (4C12)_PCR/1268 [Gene]SCO6025/1160 NC_003888 SC1C3.13, unknown, len: 655 aa; C-terminus is similar to upstream genes TR:O69849 (EMBL:AL023702) SC1C3.07 (opt: 399 Z-score: 441.0 E(): 6.2e-17; 31.751% identity in 337 aa overlap) and TR:O69848 (EMBL:AL023702) SC1C3.06 (opt: 428 Z-score: 473.3 E(): 9.9e-19; 32.022% identity in 356 aa overlap) 1 4 1 16 [Reporter]SCO2175 (6I17)_PCR/6062 [Gene]SCO2175/5519 NC_003888 SC5F7.26, possible nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, len: 357 aa; similar to SW:COBT_SALTY nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, cobalamin (vitamin B12) biosynthetic gene from Salmonella typhimurium (356 aa) fasta scores; opt: 509, z-score: 554.1, E(): 1.6e-23, (32.3% identity in 347 aa overlap). Also similar to SW:COBT_MYCTU putative nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase from Mycobacterium tuberculosis (361 aa) fasta scores; opt: 939, z-score: 1016.0, E(): 0, (44.9% identity in 334 aa overlap). 1 4 1 15 [Reporter]SCO2664 (7E17)_PCR/6061 [Gene]SCO2664/5518 NC_003888 SC6D10.07, possible sugar-binding protein, len: 352 aa; similar to TR:O34563 (EMBL:Z99117) Bacillus subtilis glutamine ABC transporter (glutamine-binding protein) GlnH, 27 33; fasta scores: opt: 474 z-score: 507.4 E(): 7.5e-21; 32.6% identity in 285 aa overlap and to SW:GLNH_BACST (EMBL:M61017) Bacillus stearothermophilus glutamine-binding protein precursor GlnH, 262 aa; fasta scores: opt: 414 z-score: 444.6 E(): 2.4e-17; 33.1% identity in 236 aa overlap. Contains Pfam match to entry PF00497 SBP_bac_3, Bacterial extracellular solute-binding proteins, family 3, to Prosite entry PS01039 Bacterial extracellular solute-binding proteins, family 3 signature and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site plus possible N-terminal region signal sequence peptide 1 4 1 14 [Reporter]SCO6852 (8A17)_PCR/6060 [Gene]SCO6852/5517 NC_003888 SC7F9.04c, unknown, len: 273 aa. Similar to other hypothetical proteins predicted to be encoded by CDS located on this cosmid: SC7F9.02c (259 aa), fasta scores opt: 442 z-score: 398.4 E(): 9.8e-17 33.7% identity in 255 aa overlap and SC7F9.06c (267 aa), fasta scores opt: 76 z-score: 373.8 E(): 2.3e-15 30.5% identity in 272 aa overlap 1 4 1 13 [Reporter]SCO5842 (9M13)_PCR/6059 [Gene]SCO5842/5516 NC_003888 SC9B10.09, unknown, len: 1039; weak similarity to hypothetical proteins from M. jannaschii Y590_METJA Q58010 hypothetical protein mj0590 (704 aa), fasta scores; opt: 377 z-score: 424.6 E(): 1.7e-16, 24.0% identity in 810 aa overlap; and E. coli YFIQ_ECOLI P76594 hypothetical 98.0 kd protein in ung-pssa intergenic region (886 aa), fasta scores; opt: 267 z-score: 215.7 E(): 7.4e-05, 23.9% identity in 833 aa overlap. Contains 12 tandem repeats of the aa triplet SGP at aa positions 695 to 707; these are not present in Y590_METJA or YFIQ_ECOLI 1 4 1 12 [Reporter]SCO2317 (10I13)_PCR/6058 [Gene]SCO2317/5515 NC_003888 SCC53.08c, possible methyltransferase, len: 244 aa; region similar to many methyltransferases e.g. TR:O82720 (EMBL:U81312) S-adenosyl-methionine-sterol-C-methyltransferase from Nicotiana tabacum (Common tobacco) (346 aa) fasta scores; opt: 228, z-score: 285.6, E(): 1.9e-08, 33.1% identity in 139 aa overlap. 1 4 1 11 [Reporter]SCO4552 (11E13)_PCR/6057 [Gene]SCO4552/5514 NC_003888 SCD16A.31, possible secreted hydrolase, len: 334aa; similar to eg. TR:Q59145 (EMBL:D31818) chitinases II precursor from Aeromonas sp. NO.10S-24 (542 aa) fasta scores; opt: 482, z-score: 492.4, E(): 4.2e-20, (36.6% identity in 273 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 1 4 1 10 [Reporter]SCO4231 (12A13)_PCR/6056 [Gene]SCO4231/5513 NC_003888 SCD8A.04c, possible lipoprotein, len: 241 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 1 4 1 9 [Reporter]SCO3107 (13M9)_PCR/6055 [Gene]SCO3107/5512 NC_003888 SCE41.16c, possible lipoprotein, len: 258 aa; similar to TR:P95123 (EMBL:Z83018) Mycobacterium tuberculosis hypothetical 25.9 kDa protein MTCY349.15, 237 aa; fasta scores: opt: 498 z-score: 531.0 E(): 4.5e-22; 44.1% identity in 186 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 2 7 13 [Reporter]SCO6070 (9H23)_PCR/3672 [Gene]SCO6070/3339 NC_003888 SC9B1.17c, possible secreted solute binding protein, len: 337aa; similarity to many eg. SW:RBSB_BACSU D-ribose binding protein from Bacillus subtilis (305 aa) fasta scores; opt: 251, z-score: 270.5, E(): 9.6e-08, (26.4% identity in 288 aa overlap). Contains possible N-terminal signal sequence and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 1 4 1 8 [Reporter]SCO0767 (14I9)_PCR/6054 [Gene]SCO0767/5511 NC_003888 SCF81.26, hypothetical protein, len: 835 aa; similar to various Streptomyces hypothetical proteins, e.g. TR:Q9ZBJ0 (EMBL:AL035161) Streptomyces coelicolor conserved hypothetical protein SC9C7.20, 860 aa; fasta scores: opt: 1329 z-score: 1414.8 E(): 0; 36.8% identity in 835 aa overlap. Contains match to Pfam entry PF01590 GAF, GAF domain and two matches to Pfam entry PF00989 PAS, PAS domain 4 2 7 12 [Reporter]SCO2723 (10D23)_PCR/3671 [Gene]SCO2723/3338 NC_003888 SCC46.08c, probable ABC transporter ATP-binding protein, len: 866 aa; C-terminal region similar to SW:YHCH_BACSU (EMBL:X96983) Bacillus subtilis hypothetical ABC transporter ATP-binding protein 2 in GlpD-CspB intergenic region, YhcH, 305 aa; fasta scores: opt: 597 z-score: 577.6 E(): 9.3e-25; 36.6% identity in 303 aa overlap and to SW:BCRA_BACLI (EMBL:L20573) Bacillus licheniformis bacitracin transport ATP-binding protein BcrA, 306 aa; fasta scores: opt: 581 z-score: 562.3 E(): 6.6e-24; 38.8% identity in 304 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Contains also possible N-terminal region signal peptide sequence 1 4 1 7 [Reporter]SCO1720 (15E9)_PCR/6053 [Gene]SCO1720/5510 NC_003888 SCI11.09, possible ABC-transporter transmembrane protein, len: 271 aa; similar to transmembrane subunits of members of the ABC-transporter superfamily e.g SW:DRRB_STRPE (EMBL:M73758), drrB, Streptomyces peucetius daunorubicin resistance transmembrane protein (283 aa), fasta scores; opt: 467 z-score: 550.2 E(): 2.6e-23, 34.0% identity in 244 aa overlap. Similar to many others from S.coelicolor e.g. SCE9.24c (EMBL:AL049841) S.coelicolor probable ABC transporter transmembrane component (260 aa) (41.8% identity in 268 aa overlap). Contains hydrophobic, possible membrane-spanning regions 4 2 7 11 [Reporter]SCO4627 (11P19)_PCR/3670 [Gene]SCO4627/3337 NC_003888 SCD39.27c, unknown, len: 89 aa 4 2 7 10 [Reporter]SCO2928 (12L19)_PCR/3669 [Gene]SCO2928/3336 NC_003888 SCE19A.28, probable asnC-family transcriptional regulator, len: 163 aa; similar to SW:ASNC_ECOLI (EMBL:K00826), AsnC, Escherichia coli transcriptional activator of asparagine synthetase (152 aa), fasta scores; opt: 236 z-score: 296.2 E(): 3.7e-09, 31.3% identity in 147 aa overlap and to putative asnC-family regulators. Similar to SC6G9.10 (EMBL:AL079356) S.coelicolor probable AsnC family transcriptional regulator (164 aa) (48.7% identity in 156 aa overlap). Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family 4 2 7 9 [Reporter]SCO0720 (13H19)_PCR/3668 [Gene]SCO0720/3335 NC_003888 SCF42.30c, possible integral membrane protein, len: 497 aa. Weakly similar to several proteins of unknown function e.g. Mycobacterium tuberculosis SW:YT19_MYCTU(EMBL:Z79700) (455 aa), fasta scores opt: 157 z-score: 177.3 E(): 0.017 27.1% identity in 450 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 2 7 8 [Reporter]SCO1820 (14D19)_PCR/3667 [Gene]SCO1820/3334 NC_003888 SCI8.05, possible integral membrane protein, len: 159 aa. Contains possible membrane spanning hydrophobic domains. 4 2 7 7 [Reporter]SCO0227 (15P15)_PCR/3666 [Gene]SCO0227/3333 NC_003888 SCJ9A.06c, unknown, len: 120aa; predicted by GC Frameplot and amino acid usage. 3 1 12 18 [Reporter]SCO2079 (5O8)_PCR/1267 [Gene]SCO2079/1159 NC_003888 SC4A10.12c, conserved hypothetical protein, len: 213 aa; unknown function, similar to hypothetical proteins e.g. TR:O06229 (EMBL:Z95388) Mycobacterium tuberculosis hypothetical protein (241 aa), fasta scores; opt: 441 z-score: 479.6 E(): 2.2e-19, 40.8% identity in 218 aa overlap. The N-terminus is Asp- and Glu-rich 4 2 7 6 [Reporter]SCO0933 (16L15)_PCR/3665 [Gene]SCO0933/3332 NC_003888 SCM10.21, possible lipoprotein, len: 172 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 3 1 12 17 [Reporter]SCO7448 (6K8)_PCR/1266 [Gene]SCO7448/1158 NC_003888 SC5C11.05, unknown, len: 647 aa. Similar to another protein of undefined function from Streptomyces coelicolor TR:Q9X8W1(EMBL:AL078610) hypothetical 66.2 KD protein, SCH35.45c (634 aa), fasta scores opt: 1537 z-score: 1606.6 E(): 0 46.3% identity in 570 aa overlap. Contains a Pfam match to entry PF00989 PAS, PAS domain. 4 2 7 5 [Reporter]SCO3412 (17H15)_PCR/3664 [Gene]SCO3412/3331 NC_003888 SCE9.19, conserved hypothetical protein, len: 106 aa; unknown function, weakly similar to e.g. TR:O26644 (EMBL:AE000837) Methanobacterium thermoautotrophicum hypothetical protein (127 aa), fasta scores; opt: 338 z-score: 369.7 E(): 2.9e-13, 50.5% identity in 107 aa overlap 3 1 12 16 [Reporter]SCO7254 (7G8)_PCR/1265 [Gene]SCO7254/1157 NC_003888 SC5H1.38, probable myo-inositol dehydrogenase, len: 342 aa; similar to many e.g. SW:MI2D_BACSU (EMBL:M76431), idh, Bacillus subtilis myo-inositol 2-dehydrogenase (344 aa), fasta scores; opt: 864 z-score: 958.5 E(): 0, 38.1% identity in 336 aa overlap. Also similar to SW:STRI_STRGR (EMBL:Y00459), strI, Streptomyces griseus streptomycin biosynthesis myo-inositol oxidoreductase (348 aa) (32.7% identity in 343 aa overlap). Weakly similar to TR:O69945 (EMBL:AL023862) S.coelicolor possible oxidoreductase (430 aa) (29.8% identity in 228 aa overlap). Contains Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family, score 153.50, E-value 3.6e-42 4 2 7 4 [Reporter]SCO2937 (18D15)_PCR/3663 [Gene]SCO2937/3330 NC_003888 SCE56.01c, possible transport protein (partial), len: >170 aa; similar to TR:AAF11647 (EMBL:AE002045) Deinococcus radiodurans conserved hypothetical protein DR2098, 462 aa; fasta scores: opt: 330 z-score: 380.0 E(): 9.3e-14; 37.4% identity in 155 aa overlap and to TR:O69070 (EMBL:AF079900) Streptomyces rimosus tetracycline efflux protein OtrB, 563 aa; fasta scores: opt: 271 z-score: 312.6 E(): 5.3e-10; 34.8% identity in 158 aa overlap. Contains possible hydrophobic membrane spanning regions,SCE19A.37c, possible transmembrane transport protein, partial CDS, len: >344 aa; weak similarity to transport proteins, many hypothetical e.g. TR:Q50392 (EMBL:U40487), lfrA, Mycobacterium smegmatis proton antiporter efflux pump (504 aa), fasta scores; opt: 220 z-score: 251.9 E(): 1.1e-06, 27.8% identity in 345 aa overlap. Weak similarity to part of others from S.coelicolor e.g. TR:Q9ZBJ1 (EMBL:AL035161) S.coelicolor probable efflux protein (815 aa) (27.2% identity in 345 aa overlap). Contains hydrophobic, possible membrane-spanning regions 3 1 12 15 [Reporter]SCO5330 (8C8)_PCR/1264 [Gene]SCO5330/1156 NC_003888 SC6G9.03c, hypothetical protein, len: 300 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 3 1 12 14 [Reporter]SCO7820 (9O4)_PCR/1263 [Gene]SCO7820/1155 NC_003888 SC8E7.17, doubtful CDS, len: 148 aa. 3 1 12 13 [Reporter]SCO2775 (10K4)_PCR/1262 [Gene]SCO2775/1154 NC_003888 SCC105.06, possible tetR-family regulatory protein, len: 208 aa; similar to TR:AAF11696 (EMBL:AE002049) Deinococcus radiodurans transcriptional regulatory protein, tetR family DR2148, 240 aa; fasta scores: opt: 189 z-score: 219.0 E(): 9.3e-05; 31.7% identity in 205 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 3 1 12 12 [Reporter]SCO2273 (11G4)_PCR/1261 [Gene]SCO2273/1153 NC_003888 SCC75A.19, FecCD-family membrane transport protein, len: 363 aa. Highly similar to proteins involved in iron acquisition including: Yersinia pestis SW:HMUU_YERPE(EMBL:U60647) hemin transport system permease protein HmuU (334 aa), fasta scores: opt: 855 z-score: 924.4 E():0 45.9% identity in 290 aa overlap and Corynebacterium diphtheriae TR:AAD37494(EMBL:AF109162) BC-type transporter permease (350 aa), fasta scores opt: 1006 z-score: 1085.8 E():0 47.2% identity in 339 aa overlap. Contains a Pfam match to entry PF01032 FecCD_family, FecCD transport family and multiple possible membrane spanning hydrophobic domains. 3 1 12 11 [Reporter]SCO4786 (12C4)_PCR/1260 [Gene]SCO4786/1152 NC_003888 SCD63.18c, unknown, len: 155 aa 3 1 12 10 [Reporter]SCO2848 (12O24)_PCR/1259 [Gene]SCO2848/1151 NC_003888 SCE20.22, unknown, len: 158 aa. Similar to several proteins of undefined function e.g. Rhizobium meliloti SW:YPMI_RHIME(EMBL:M96584) hypothetical 18.2 KD protein (164 aa), fasta scores opt: 434 z-score: 540.5 E(): 1e-22 49.7% identity in 143 aa overlap. Also similar to Saccharomyces cerevisiae SW:YBY7_YEAST(EMBL:X75891) hypothetical 20.5 KD protein (179 aa), fasta scores opt: 278 z-score: 350.5 E(): 3.8e-12 38.8% identity in 152 aa overlap 3 1 12 8 [Reporter]SCO1898 (14G24)_PCR/1257 [Gene]SCO1898/1150 NC_003888 SCI7.16, possible sugar binding protein, len: 456aa; similar to many eg. TR:O30831 (EMBL:AF018073) periplasmic sorbitol binding protein from Rhodobacter sphaeroides (436 aa) fasta scores; opt: 1203, z-score: 1347.3, E(): 0, (42.8% identity in 423 aa overlap). Contains possible N-terminal signal sequence. 1 4 1 6 [Reporter]SCO0817 (16A9)_PCR/6052 [Gene]SCO0817/5509 NC_003888 SCF43A.07, hypothetical protein, len: 215 aa; unknown function, weakly similar to TR:O32259 (EMBL:Z99121) Bacillus subtilis hypothetical protein (240 aa), fasta scores; opt: 185 z-score: 226.3 E(): 2.9e-05, 27.6% identity in 232 aa overlap and to TR:Q9RT57 (EMBL:AE002030) Deinococcus radiodurans conserved hypothetical protein DR1909, 212 aa, fasta scores: opt: 490 Z-score: 562.1 E(): 1.1e-23; 46.893% identity in 177 aa overlap 1 4 1 5 [Reporter]SCO3380 (17M5)_PCR/6051 [Gene]SCO3380/5508 NC_003888 SCE94.31c, unknown, len: 265aa; similar to hypothetical proteins eg. TR:O06282 (EMBL:Z95557) from Mycobacterium tuberculosis (272 aa) fasta scores; opt: 884, z-score: 1015.5, E(): 0, (51.9% identity in 268 aa overlap). 1 4 1 4 [Reporter]SCO0626 (18I5)_PCR/6050 [Gene]SCO0626/5507 NC_003888 SCF56.10c, unknown, len: 214 aa 1 4 1 3 [Reporter]SCO4002 (19E5)_PCR/6049 [Gene]SCO4002/5506 NC_003888 2SC10A7.06, possible secreted protein, len: 108 aa. Contains possible N-terminal region signal peptide sequence 4 3 23 22 [Reporter]SCO4939 (1L22)_PCR/6047 [Gene]SCO4939/5505 NC_003888 SCK13.31c, unknown, len: 260 aa 4 3 23 21 [Reporter]SCO1508 (2H22)_PCR/6046 [Gene]SCO1508/5504 NC_003888 SC9C5.32c, hisS, histidyl tRNA synthetase, len: 425 aa; similar to SW:SYH_ECOLI (EMBL:M11843) Escherichia coli histidyl tRNA synthetase (EC 6.1.1.21) HisS, 423 aa; fasta scores: opt: 1054 z-score: 1173.7 E(): 0; 42.3% identity in 421 aa overlap. Contains Pfam match to entry PF00587 tRNA-synt_2b, tRNA synthetase class II (G, H, P, S and T) 4 3 23 20 [Reporter]SCO7392 (3D22)_PCR/6045 [Gene]SCO7392/5503 NC_003888 SC10G8.20, unknown, len: 163 aa. 4 3 23 19 [Reporter]SCO7614 (4P18)_PCR/6044 [Gene]SCO7614/5502 NC_003888 SC2H2.12c, possible regulatory protein, len: 173 aa; similar to TR:Q9L1E5 (EMBL:AL157916) Streptomyces coelicolor putative AbaA-like regulatory protein SC3D11.17, 220 aa; fasta scores: opt: 232 z-score: 287.6 E(): 1.8e-08; 41.1% identity in 129 aa overlap. Contains TTA leucine codon (37) possible target for bldA regulation 4 2 7 3 [Reporter]SCP1.05c (19P11)_PCR/3662 [Gene]SCP1.05c/3329 NC_003888 SCP1.05c, unknown, len: 131aa; 4 3 23 18 [Reporter]SCO6706 (5L18)_PCR/6043 [Gene]SCO6706/5501 NC_003888 SC4C6.16, probable glutathione-dependent aldehyde dehydrogenase, len: 396 aa; similar to many e.g. SW:FADH_METMR (EMBL:L33464), fdh, Methylobacter marinus glutathione-dependent formaldehyde dehydrogenase (424 aa), fasta scores; opt: 1126 z-score: 1211.8 E(): 0, 44.1% identity in 406 aa overlap. Similar to (EMBL:AL049485), SC6A5.31c, S.coelicolor probable zinc-binding alcohol dehydrogenase (363 aa) (31.0% identity in 316 aa overlap). Contains Pfam match to entry PF00107, adh_zinc, Zinc-binding dehydrogenases and PS00059 Zinc-containing alcohol dehydrogenases signature 4 2 7 2 [Reporter]SCO1855 (20H11)_PCR/3661 [Gene]SCO1855/3328 NC_003888 SCI39.02, probable precorrin-4 C11-methyltransferase, len: 281 aa; similar to TR:O87696 (EMBL:AJ000758) Bacillus megaterium precorrin-4 methylase CbiF, 258 aa; fasta scores: opt: 674 Z-score: 771.2 bits: 150.4 E(): 2.3e-35; 41.502% identity in 253 aa overlap and to SW:COBM_PSEDE (EMBL:M59301) Pseudomonas denitrificans precorrin-4 C11-methyltransferase (EC 2.1.1.133) CobM, 253 aa; fasta scores: opt: 652 Z-score: 746.5 bits: 145.8 E(): 5.5e-34; 43.145% identity in 248 aa overlap . Contains Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases 4 3 23 17 [Reporter]SCO7558 (6H18)_PCR/6042 [Gene]SCO7558/5500 NC_003888 SC5F1.12, probable beta-glucosidase, len: 479 aa; similar to TR:Q59976 (EMBL:Z29625) Streptomyces sp. beta-glucosidase (EC 3.2.1.21) Bgl3, 479 aa; fasta scores: opt: 2961 z-score: 3301.3 E(): 0; 88.9% identity in 479 aa overlap and to TR:CAC10107 (EMBL:AL442165) Streptomyces coelicolor putative cellobiose hydrolase 2SCC13.06, 479 aa; fasta scores: opt: 2255 z-score: 2227.1 E(): 0; 67.4% identity in 470 aa overlap. Contains Pfam match to entry PF00232 Glyco_hydro_1, Glycosyl hydrolase family 1 and match to Prosite entry PS00653 Glycosyl hydrolases family 1 N-terminal signature and PS00572 Glycosyl hydrolases family 1 active site 4 2 6 22 [Reporter]SCO5246 (1H7)_PCR/3659 [Gene]SCO5246/3327 NC_003888 2SC7G11.08, possible Na+/H+ antiporter, len: 530 aa; identical to C-terminal region of previously sequenced TR:Q9RPC9 (EMBL:AF162936) Streptomyces coelicolor A3(2) Na/H antiporter homolog (fragment), 212 aa and similar to SW:YJCE_ECOLI: (EMBL:U00006) Escherichia coli putative Na(+)/H(+) exchanger YjcE, 549 aa; fasta scores: opt: 516 z-score: 557.0 E(): 1.5e-23; 30.1% identity in 552 aa overlap. Contains Pfam match to entry PF00999 Na_H_Exchanger, Sodium/hydrogen exchanger family and possible hydrophobic membrane spanning regions 4 2 6 21 [Reporter]SCO2779 (2D7)_PCR/3658 [Gene]SCO2779/3326 NC_003888 SCC105.10, acdH, acyl-CoA dehydrogenase, len: 386 aa; identical to previously sequenced TR:Q9XCG6 (EMBL:AF142581) Streptomyces coelicolor acyl-CoA dehydrogenase AcdH, 386 aa. Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase and matches to Prosite entries PS00072 Acyl-CoA dehydrogenases signature 1 and PS00073 Acyl-CoA dehydrogenases signature 2 4 2 6 20 [Reporter]SCO4712 (3P3)_PCR/3657 [Gene]SCO4712/3325 NC_003888 SCD31.37, rplN, 50S ribosomal protein L14, len: 122 aa; highly similar to SW:RL14_BACST (PIR:A02789) Bacillus stearothermophilus 50S ribosomal protein L14 RplN, 122 aa; fasta scores: opt: 630 z-score: 783.7 E(): 0; 77.9% identity in 122 aa overlap. Contains Pfam match to entry PF00238 Ribosomal_L14, Ribosomal protein L14 and match to Prosite entry PS00049 Ribosomal protein L14 signature 4 2 6 19 [Reporter]SCO6504 (4L3)_PCR/3656 [Gene]SCO6504/3324 NC_003888 SC1E6.13, unknown, len: 376 aa; some similarity in part to YMEL_STRLN Streptomyces lincolnensis hypothetical protein (150 aa), fasta scores; opt: 377 z-score: 448.8 E(): 1e-17, 36.1% identity in 144 aa overlap. Contains highly acidic repetitive region from aa 290-376 4 2 6 18 [Reporter]SCO6352 (5H3)_PCR/3655 [Gene]SCO6352/3323 NC_003888 SC3A7.20c, unknown, len: 138 aa 3 1 12 7 [Reporter]SCO0477 (15C24)_PCR/1256 [Gene]SCO0477/1149 NC_003888 SCF76.17, conserved hypothetical protein, len: 130 aa. Highly similar to several including: Bacillus subtilis TR:O32161 (EMBL; Z99120) YurT protein (127 aa), fasta scores opt: 494 z-score: 605.8 E(): 2.2e-26 58.6% identity in 128 aa overlap, Streptomyces coelicolor TR:CAB42950 (EMBL; AL049863) hypothetical 14.4 KD protein SC5H1.25C (131 aa), fasta scores opt: 221 z-score: 280.0 E(): 3.1e-08 37.2% identity in 129 aa overlap and also weakly similar to some lyases e.g. Salmonella typhimurium SW:LGUL_SALTY (EMBL; U57364) lactoylglutathione lyase (EC 4.4.1.5) (135 aa), fasta scores opt: 101 z-score: 136.7 E(): 2.9 23.1% identity in 134 aa overlap 4 2 6 17 [Reporter]SCO6637 (6D3)_PCR/3654 [Gene]SCO6637/3322 NC_003888 SC4G2.11c, unknown, len: 350 aa; identical to TR:Q53944 (EMBL:L37531) ORF3 downstream of PglZ (350 aa) 3 1 12 6 [Reporter]SCO1653 (16O20)_PCR/1255 [Gene]SCO1653/1148 NC_003888 SCI41.36, hypothetical protein, len: 371aa. Similar to various hypothetical proteins, e.g. TR:Q9ZG15 (EMBL:AF088800) Rhodococcus erythropolis hypothetical 35.5 Kd protein (323 aa); fasta scores: opt: 1017 z-score: 1034.8 E(): 0; 51.5% identity in 299 aa overlap. 4 2 6 16 [Reporter]SCO4849 (6P23)_PCR/3653 [Gene]SCO4849/3321 NC_003888 SC5G8.17, possible integral membrane protein, len: 436 aa; N-terminal region contains possible membrane-spanning hydrophobic regions while the remainder is similar to many hypothetical proteins e.g. TR:Q9ZM43 (EMBL:AE001473) putative from Helicobacter pylori J99 (370 aa) fasta scores; opt: 596, z-score: 590.7, E(): 1.9e-25, 37.4% identity in 273 aa overlap 3 1 12 5 [Reporter]SCO3337 (17K20)_PCR/1254 [Gene]SCO3337/1147 NC_003888 SCE7.04c, proC, pyrroline-5-carboxylate reductase, len: 284aa; highly similar to many from prokaryotes and eukaryotes egs. SW:PROC_CORGL proC, pyrroline-5-carboxylate reductase from Corynebacterium glutamicum (270 aa) fasta scores; opt: 735, z-score: 832.5, E(): 0, (46.2% identity in 264 aa overlap) and SW:PROC_ARATH proC, pyrroline-5-carboxylate reductase from Arabidopsis thaliana (276 aa) fasta scores; opt: 604, z-score: 685.7, E(): 7.1e-31, (41.1% identity in 265 aa overlap). Contains Pfam match to entry PF01089 P5CR, Delta 1-pyrroline-5-carboxylate reductase and Prosite match to PS00521 Delta 1-pyrroline-5-carboxylate reductase signature. 4 2 6 15 [Reporter]SCO6972 (7L23)_PCR/3652 [Gene]SCO6972/3320 NC_003888 SC6F7.25c, unknown, len: 179 aa. 3 1 12 4 [Reporter]SCO3916 (18G20)_PCR/1253 [Gene]SCO3916/1146 NC_003888 SCH24.38, conserved hypothetical protein, len: 139 aa; similar to many e.g. SW:YDHY_CITFR (EMBL:U09771) Citrobacter freundii hypothetical protein (142 aa), fasta scores; opt: 257 z-score: 321.3 E(): 1.4e-10, 35.4% identity in 130 aa overlap 3 1 12 3 [Reporter]SCP1.255c (19C20)_PCR/1252 [Gene]SCP1.255c/1145 NC_003888 SCP1.255c, unknown, len: 205aa; 3 1 12 2 [Reporter]SCO3980 (20K16)_PCR/1251 [Gene]SCO3980/1144 NC_003888 SCBAC25E3.17c, unknown, len: 191 aa: no significant database matches. 3 1 11 22 [Reporter]SCO1271 (1K12)_PCR/1249 [Gene]SCO1271/1143 NC_003888 2SCG18.18c, fabH3, probable 3-oxoacyl-[acyl-carrier-protein] synthase III, len: 317 aa; similar to TR:FABH_ECOLI (EMBL:M77744) Escherichia coli 3-oxoacyl-[acyl-carrier-protein] synthase III (KASIII) FabH, 317 aa; fasta scores: opt: 526 z-score: 548.1 E(): 4.8e-23; 30.2% identity in 318 aa overlap and to TR:Q9ZBV4 (EMBL:AL034443) Streptomyces coelicolor FabH2, 3-oxoacyl-[acyl-carrier-protein] synthase IIe SC4B5.14, 316 aa; fasta scores: opt: 701 z-score: 636.7 E(): 7.2e-30; 38.7% identity in 323 aa overlap 3 1 11 21 [Reporter]SCO4645 (2G12)_PCR/1248 [Gene]SCO4645/1142 NC_003888 SCD82.16c, aspC, aspartate aminotransferase, len: 408 aa; highly similar to SW:AAT_STRVG (EMBL:D50624) Streptomyces virginiae aspartate aminotransferase AspC, 397 aa; fasta scores: opt: 2346 z-score: 2655.6 E(): 0; 89.4% identity in 396 aa overlap. Contains Pfam match to entry PF00155 aminotran_1, Aminotransferases class-I and match to Prosite entry PS00105 Aminotransferases class-I pyridoxal-phosphate attachment site 3 1 11 20 [Reporter]SCO6684 (3C12)_PCR/1247 [Gene]SCO6684/1141 NC_003888 SC5A7.34, ramB, probable ABC transporter, len: 608 aa; N-terminus contains transmembrane domain, C-terminus contains ATP-binding domain. IdenticalTR:Q53819 (EMBL:U03771) potential ATP-binding membrane protein RamB (608 aa) and similar tyo many e.g. Y07D_MYCTU hypothetical ABC transporter ATP-binding protein MTCY02B10.13 (579 aa), fasta scores; opt: 456 z-score: 443.0 E(): 1.9e-17, 31.8% identity in 614 aa overlap. Contains sixteen degenerate PE repeats between the N- andC-terminal domains. Also contains PS00017 ATP /GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature and Pfam match to entry PF00005 ABC_tran, ABC transporters, score 157.10, E-value 3e-43. Some similarity to RamA (E(): 2.7e-05, 33.4% identity in 628 aa overlap) 3 1 11 18 [Reporter]SCO2075 (5K8)_PCR/1245 [Gene]SCO2075/1140 NC_003888 SC4A10.08c, probable DNA-binding protein, len: 239 aa; the N-terminus is Lys- and Ala-rich, containing 13 degenerate AAKK(S)(P/T) repeats and is similar to histone-like proteins e.g. SW:KARP_CHLTR (EMBL:M86605), KarP, Chlamydia trachomatis developmentally regulated gene (207 aa), fasta scores; opt: 245 z-score: 253.5 E(): 8.5e-07, 44.7% identity in 114 aa overlap. This region is also similar to a repeat in TR:O86537 (EMBL:AL031124) S.coelicolor histone-like DNA binding protein (218 aa) (51.4% identity in 72 aa overlap). The C-terminus is similar to SW:DKSA_ECOLI (EMBL:M34945), DskA, Escherichia coli DnaK suppressor protein (151 aa) (28.8% identity in 125 aa overlap), a dosage-dependent suppressor of dnaK deletion mutants. Contains probable coiled-coil from 123 to 159 (37 residues) (Max score: 1.709, probability 1.00). Contains Pfam match to entry PF01258 zf_dskA_traR, Prokaryotic dksA/traR C4-type zinc finger and PS01102 Prokaryotic dksA/traR C4-type zinc finger 4 2 6 13 [Reporter]SCO6066 (9D23)_PCR/3650 [Gene]SCO6066/3319 NC_003888 SC9B1.13c, cvnD6, possible ATP/GTP-binding protein, len: 181aa; similar to others egs. TR:O50391 (EMBL:AL009198) hypothetical protein from Mycobacterium tuberculosis (193 aa) fasta scores; opt: 689, z-score: 849.1, E(): 0, (61.4% identity in 166 aa overlap) and two putative ATP/GTP-binding proteins from Streptomyces coelicolor: TR:O86519 (EMBL:AL031124) (174 aa) fasta scores; opt: 665, z-score: 820.6, E(): 0, (55.0% identity in 171 aa overlap) and TR:CAA19989 (EMBL:AL031124) (174 aa) fasta scores; opt: 662, z-score: 817.0, E(): 0, (55.6% identity in 171 aa overlap). Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 2 6 12 [Reporter]SCO2741 (10P19)_PCR/3649 [Gene]SCO2741/3318 NC_003888 SCC57A.12, possible secreted protein, len: 169 aa. Contains a possible N-terminal signal sequence. 4 2 6 11 [Reporter]SCO4622 (11L19)_PCR/3648 [Gene]SCO4622/3317 NC_003888 SCD39.22c, possible integral membrane protein, len: 166 aa. Contains possible hydrophobic membrane spanning regions 4 2 6 10 [Reporter]SCO4301 (12H19)_PCR/3647 [Gene]SCO4301/3316 NC_003888 SCD95A.34c, possible DNA-binding protein, len: 279 aa; similar to various S. coelicolor hypothetical proteins, e.g. TR:CAB69734 (EMBL:AL137166) Streptomyces coelicolor hypothetical 30.4 kD protein SCC121.16, 277 aa; fasta scores: opt: 731 z-score: 897.3 E(): 0; 45.2% identity in 259 aa overlap and to TR:CAB88448 (EMBL:AL353815) Streptomyces coelicolor putative DNA-binding protein SCD6.19, 295 aa; fasta scores: opt: 388 z-score: 478.8 E(): 3.3e-19; 34.3% identity in 286 aa overlap. Contains TTA leucine codon, possible target for bldA regulation and a possible helix-turn-helix motif at residues 30..51 (+3.39 SD) 4 2 6 9 [Reporter]SCO3379 (13D19)_PCR/3646 [Gene]SCO3379/3315 NC_003888 SCE94.30c, unknown, proline-rich, len: 208aa; 4 2 6 8 [Reporter]SCO0816 (14P15)_PCR/3645 [Gene]SCO0816/3314 NC_003888 SCF43A.06, conserved possible iron-sulfur protein, len: 492 aa; similar to several putative iron-sulfur proteins e.g. SW:YKGF_ECOLI (EMBL:AE000137) Escherichia coli hypothetical protein (475 aa), fasta scores; opt: 1148 z-score: 1249.5 E(): 0, 39.7% identity in 481 aa overlap, which contains 2 putative 4Fe-4S centres. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature 4 2 6 7 [Reporter]SCO2938 (15L15)_PCR/3644 [Gene]SCO2938/3313 NC_003888 SCE56.02, possible oxidoreductase, len: 336 aa; similar to TR:CAB61562 (EMBL:AL133171) Streptomyces coelicolor putative aldo/keto reductase (fragment) SCF81.28c, 307 aa; fasta scores: opt: 1370 z-score: 1572.6 E(): 0; 65.2% identity in 305 aa overlap and to SW:NORA_ASPFL (EMBL:U32377) Aspergillus flavus norsolorinic acid reductase (EC 1.1.1.-) NorA, 388 aa; fasta scores: opt: 572 z-score: 659.1 E(): 2.7e-29; 32.8% identity in 329 aa overlap 3 1 11 17 [Reporter]SCO2062 (6G8)_PCR/1244 [Gene]SCO2062/1139 NC_003888 SC4G6.31c, unknown, len: 403aa; 4 2 6 6 [Reporter]SCO3229 (16H15)_PCR/3643 [Gene]SCO3229/3312 NC_003888 SCE63.04, probable 4-hydroxyphenylpyruvic acid dioxygenase, len: 371 aa; similar to many both prokaryotic and eukaryotic e.g. TR:O52791 (EMBL:AJ223998) similar to hydroxyphenyl pyruvate dioxygenase from a cluster of genes involved in the biosynthesis of a vancomycin group antibiotic in Amycolatopsis orientalis (Actinomycete) (357 aa) fasta scores; opt: 989, z-score: 1130.3, E(): 0, (48.7% identity in 355 aa overlap) and SW:HPPD_MOUSE hpd, 4-hydroxyphenylpyruvic acid dioxygenase from Mus musculus (mouse) (392 aa) fasta scores; opt: 610, z-score: 698.5, E(): 1.3e-31, (31.6% identity in 361 aa overlap). 3 1 11 16 [Reporter]SCO7258 (7C8)_PCR/1243 [Gene]SCO7258/1138 NC_003888 SC5H1.34, conserved hypothetical protein, len: 161 aa; unknown function, similar to hypothetical proteins e.g. TR:O59834 (EMBL:AL023590) Schizosaccaromyces pombe hypothetical protein (162 aa), fasta scores; opt: 224 z-score: 282.3 E(): 2.1e-08, 28.6% identity in 161 aa overlap and weakly similar to SW:FCA1_CANAL (EMBL:U55194), fca1, Candida albicans cytosine deaminase (150 aa) (30.1% identity in 93 aa overlap) 4 2 6 5 [Reporter]SCO0443 (17D15)_PCR/3642 [Gene]SCO0443/3311 NC_003888 SCF51A.21, unknown, len: 285 aa. Similar to a number of hypothetical proteins e.g. Mycobacterium tuberculosis TR:P95220 (EMBL; Z86089) hypothetical 32.2 KD protein (300 aa), fasta scores opt: 714 z-score: 663.0 E(): 1.4e-29 45.3% identity in 289 aa overlap. Also similar in parts to Pseudomonas fluorescens TR:CAB48407 (EMBL; AJ243652) urea amidolyase homologue (1213 aa), fasta scores opt: 423 z-score: 390.1 E(): 2.3e-14 37.7% identity in 289 aa overlap 3 1 11 15 [Reporter]SCO7239 (8O4)_PCR/1242 [Gene]SCO7239/1137 NC_003888 SC7A12.06, unknown, len: 300 aa. Weakly similar to several proteins of undefined function e.g. Mycobacterium tuberculosis TR:P94979(EMBL:Z85982) hypothetical 39.9 kd protein (351 aa), fasta scores opt: 271 z-score: 321.1 E(): 2.1e-10 26.2% identity in 309 aa overlap. Also similar to TR:Q9HV65 (EMBL:AE004887) Pseudomonas aeruginosa hypothetical protein PA4734, 293 aa; fasta scores: opt: 497 Z-score: 565.3 E(): 7.4e-24; 40.727% identity in 275 aa overlap 4 2 6 4 [Reporter]SCO0889 (18P11)_PCR/3641 [Gene]SCO0889/3310 NC_003888 SCM1.22c, possible LysR-family transcriptional regulatory protein, len: 301 aa. Similar to many other regulatory proteins including: Escherichia coli SW:OXYR_ECOLI (EMBL:J04553) hydrogen peroxide-inducible genes activator (305 aa), fasta scores opt: 348 z-score: 401.9 E(): 4.8e-15 28.4% identity in 299 aa overlap and Streptomyces coelicolor TR:O86805 (EMBL:AL031317) putative transcriptional regulator (306 aa), fasta scores opt: 491 z-score: 563.9 E(): 4.6e-24 36.4% identity in 302 aa overlap. Contains a Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family, with the putative helix-turn-helix motif situated between residues 17..38 (+3.88 SD). 3 1 11 14 [Reporter]SCO7816 (9K4)_PCR/1241 [Gene]SCO7816/1136 NC_003888 SC8E7.13, possible membrane protein, len: 234 aa. Weakly similar to several e.g. Staphylococcus aureus plasmid TR:O87368(EMBL:AF051917) membrane protein (248 aa), fasta scores opt: 177 z-score: 205.1 E(): 0.0006 20.7% identity in 237 aa overlap. Contains possible membrane spanning hydrophobic domains. 3 1 11 13 [Reporter]SCO5128 (10G4)_PCR/1240 [Gene]SCO5128/1135 NC_003888 SC9E12.13, possible membrane protein, len: 775 aa; highly hydrophilic. Contains possible hydrophobic membrane spanning region 3 1 11 12 [Reporter]SCO2269 (11C4)_PCR/1239 [Gene]SCO2269/1134 NC_003888 SCC75A.15c, unknown, len: 275 aa. 3 1 11 11 [Reporter]SCO4443 (11O24)_PCR/1238 [Gene]SCO4443/1133 NC_003888 SCD6.21c, possible merR-family transcriptional regulator, len: 294 aa; similar to TR:AAF11989 (EMBL:AE002074) Deinococcus radiodurans transcriptional regulator, merR-family DR2448, 280 aa; fasta scores: opt: 465 z-score: 562.4 E(): 7.3e-24; 43.0% identity in 286 aa overlap. Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and match to Prosite entry PS00552 Bacterial regulatory proteins, merR family signature 3 1 11 10 [Reporter]SCO2843 (12K24)_PCR/1237 [Gene]SCO2843/1132 NC_003888 SCE20.17c, nagD, probable N-acetylglucosamine-6-phosphate deacetylase, len: 259 aa. Highly similar to Escherichia coli SW:NAGD_ECOLI(EMBL:X14135) possible N-acetylglucosamine-6-phosphate deacetylase NagD (250 aa), fasta scores opt: 740 z-score: 847.6 E(): 0 43.0% identity in 249 aa overlap. 3 1 11 9 [Reporter]SCO5169 (13G24)_PCR/1236 [Gene]SCO5169/1131 NC_003888 SCP8.32c, possible ATP-binding protein, len: 94 aa. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 11 8 [Reporter]SCO1742 (14C24)_PCR/1235 [Gene]SCO1742/1130 NC_003888 SCI11.31, probable ABC-transporter ATP-binding protein, len: 319 aa; similar to SW:BCRA_BACLI (EMBL:L20573), bcrA, Bacillus licheniformis bacitracin transport ATP-binding protein (306 aa), fasta scores; opt: 561 z-score: 595.7 E(): 7.5e-26, 36.2% identity in 298 aa overlap and to many putative ABC-transporter ATP-binding proteins. Similar to others from S.coelicolor e.g. SCE8.17C (EMBL:AL035654) S.coelicolor probable ABC transporter ATP-binding protein (317 aa) (45.9% identity in 296 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and PS00017 ATP/GTP-binding site motif A (P-loop) 4 2 6 3 [Reporter]SCO4053 (19L11)_PCR/3640 [Gene]SCO4053/3309 NC_003888 2SCD60.19, possible transport integral membrane protein, len: 589 aa; similar to TR:P94432 (EMBL:D50453) Bacillus subtilis homologue of copper export protein YcnJ, 541 aa; fasta scores: opt: 394 z-score: 369.0 E(): 5.4e-13; 27.8% identity in 442 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 6 2 [Reporter]SCO6093 (20D11)_PCR/3639 [Gene]SCO6093/3308 NC_003888 SCBAC1A6.17c, possible secreted protein, len: 248 aa; similar to TR:Q9L2E8 (EMBL:AL137187) Streptomyces coelicolor putative secreted protein SC7A8.10c, 274 aa; fasta scores: opt: 552 z-score: 639.4 E(): 4.9e-28; 45.7% identity in 234 aa overlap. Contains possible cleavable N-terminal region signal peptide sequence 4 2 5 22 [Reporter]SCO5396 (1D7)_PCR/3637 [Gene]SCO5396/3307 NC_003888 2SC6G5.40c, probable cellulose-binding protein, len: 310aa; strongly similar to TR: (EMBL:) AbpS avicel-binding protein from Streptomyces reticuli (311 aa) fasta scores; opt: 1777, z-score: 1598.0, E(): 0, 93.2% identity in 311 aa overlap. Contains a possible membrane-spanning hydrophobic region close to the N-terminus and possible coiled-coil regions throughout. 4 2 5 21 [Reporter]SCO5961 (2P3)_PCR/3636 [Gene]SCO5961/3306 NC_003888 SC7H1.31c, cbiM, probably involved in cobalt transport, len: 257 aa; similar to many eg. CBIM_SALTY Q05594 cbim protein. salmonella typhimurium. (245 aa), fasta scores; opt: 749 z-score: 1155.1 E(): 0, 53.6% identity in 224 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature 4 2 5 20 [Reporter]SCO4652 (3L3)_PCR/3635 [Gene]SCO4652/3305 NC_003888 SCD82.23, rplJ, 50S ribosomal protein L10, len: 176 aa; identical to previously sequenced SW:RL10_STRCO (EMBL:L24552) Streptomyces coelicolor 50S ribosomal protein L10 RplJ. Contains Pfam match to entry PF00466 Ribosomal_L10, Ribosomal protein L10 and match to Prosite entry PS01109 Ribosomal protein L10 signature 4 2 5 19 [Reporter]SCO6907 (4H3)_PCR/3634 [Gene]SCO6907/3304 NC_003888 SC1B2.13, possible DNA ligase, len: 257 aa. Similar in parts to Archaeoglobus fulgidus SW:DNLI_ARCFU(EMBL:AE001061) DNA ligase (EC 6.5.1.1) (556 aa), fasta scores opt: 175 z-score: 199.4 E(): 0.0012 28.9% identity in 173 aa overlap and to Streptomyces coelicolor TR:Q9XAM3(EMBL:AL079355) putative DNA ligase, SC4C6.17C (355 aa), fasta scores opt: 366 z-score: 413.9 E(): 1.4e-15 32.9% identity in 237 aa overlap. 4 2 5 18 [Reporter]SCO6348 (5D3)_PCR/3633 [Gene]SCO6348/3303 NC_003888 SC3A7.16c, putative membrane protein, len: 1361 aa. Contains possible hydrophobic membrane spanning regions 3 1 11 7 [Reporter]SCO0602 (15O20)_PCR/1234 [Gene]SCO0602/1129 NC_003888 SCF55.26, hypothetical protein, len: aa; similar to various hypothetical proteins, e.g. TR:CAB42732 (EMBL:AL049826) Streptomyces coelicolor hypothetical 42.9 KD protein SCH24.26c, 373 aa; fasta scores: opt: 1649 z-score: 1932.2 E(): 0; 61.8% identity in 374 aa overlap and low similarity to TR:O51533 (EMBL:AE001160) Borrelia burgdoferi FemA protein (FemA) BB0586, 347 aa; fasta scores: opt: 307 z-score: 363.4 E(): 7.2e-13; 27.4% identity in 197 aa overlap 4 2 5 17 [Reporter]SCO7072 (5P23)_PCR/3632 [Gene]SCO7072/3302 NC_003888 SC4G1.38c, hypothetical protein, len: 199 aa; similar to SW:YCGS_ECOLI (EMBL:AE000218) Escherichia coli hypothetical 22.6 kDa protein in TreA-Pth intergenic region YcgS, 210 aa; fasta scores: opt: 349 z-score: 387.3 E(): 4.2e-14; 44.5% identity in 200 aa overlap and to C-terminal region of SW:DAK1_YEAST (EMBL:Z38114) Saccharomyces cerevisiae dihydroxyacetone kinase 1 (EC 2.7.1.29) Dak1, 584 aa; fasta scores: opt: 298 z-score: 326.4 E(): 1e-10; 33.3% identity in 189 aa overlap 3 1 11 6 [Reporter]SCO0041 (16K20)_PCR/1233 [Gene]SCO0041/1128 NC_003888 SCJ4.22, possible integral membrane protein, len: 161 aa; contains possible membrane spanning hydrophobic regions. 4 2 5 16 [Reporter]SCO4845 (6L23)_PCR/3631 [Gene]SCO4845/3301 NC_003888 SC5G8.13, possible integral membrane protein, len: 157aa; similar to many eg. SW:P72055 (Y1I9_MYCTU) hypothetical protein from Mycobacterium tuberculosis (121 aa) fasta scores; opt: 139, z-score: 176.2, E(): 0.023, 31.7% identity in 126 aa overlap. Contains possible membrane-spanning hydrophobic regions. 3 1 11 5 [Reporter]SCO0800 (17G20)_PCR/1232 [Gene]SCO0800/1127 NC_003888 SCF43.11c, possible TetR-family transcriptional regulatory protein, len: 205 aa. Similar to several including: Escherichia coli SW:ACRR_ECOLI (EMBL:U00734) potential AcrAB operon repressor AcrR (215 aa), fasta scores opt: 176 z-score: 223.7 E(): 4.5e-05 27.5% identity in 189 aa overlap and Streptomyces coelicolor TR:Q9XAE5 (EMBL:AL079356) hypothetical 24.2 KD protein SC6G9.37 (217 aa), fasta scores opt: 282 z-score: 351.9 E(): 3.3e-12 33.2% identity in 196 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 43..64 (+5.17 SD). 4 2 5 15 [Reporter]SCO1437 (7H23)_PCR/3630 [Gene]SCO1437/3300 NC_003888 SC6D7.02, possible membrane protein, len: 156 aa. Weakly similar to a hypothetical protein from Mycobacterium tuberculosis: SW:YE17_MYCTU(EMBL:Z80108) (154 aa), fasta scores opt: 212 z-score: 266.9 E(): 1.7e-07 32.4% identity in 136 aa overlap. Contains possible membrane spanning hydrophobic domains. 3 1 11 4 [Reporter]SCO3228 (18C20)_PCR/1231 [Gene]SCO3228/1126 NC_003888 SCE63.05, probable glycolate oxidase, len: 377 aa; similar to many both prokaryotic and eukaryotic e.g. TR:O52792 (EMBL:AJ223998) similar to glycolate oxidase from a cluster of genes nvolved in the biosynthesis of a vancomycin group antibiotic in Amycolatopsis orientalis (Actinomycete) (357 aa) fasta scores; opt: 995, z-score: 998.4, E(): 0, (47.5% identity in 343 aa overlap) and SW:GOX_SPIOL glycolate oxidase from Spinacia oleracea (spinach) (369 aa) fasta scores; opt: 917, z-score: 920.7, E(): 0, (41.9% identity in 360 aa overlap). Contains Pfam match to entry PF01070 FMN_dh, FMN-dependent de hydrogenase, score 412.60, E-value 3.6e-120 and PS00557 FMN-dependent alpha-hydroxy acid dehydrogenases active site. 3 1 11 3 [Reporter]SCP1.22c (19O16)_PCR/1230 [Gene]SCP1.22c/1125 NC_003888 SCP1.22c, unknown, len: 77aa; 3 1 11 2 [Reporter]SCO3982 (20G16)_PCR/1229 [Gene]SCO3982/1124 NC_003888 SCBAC25E3.19, conserved hypothetical protein, len: 92 aa: strongly similar to TR:Q9X8E1 (EMBL:AL049573) hypothetical protein SCE39.18c from Streptomyces coelicolor (92 aa) fasta scores; opt: 471, Z-score: 618.1, 81.522% identity in 92 aa overlap. Contains TTA leucine codon, possible target for bldA regulation 3 1 10 21 [Reporter]SCO1495 (2C12)_PCR/1226 [Gene]SCO1495/1123 NC_003888 SC9C5.19c, aroK, shikimate kinase I, len: 171 aa; similar to SW:AROK_ECOLI (EMBL:X80167) Escherichia coli shikimate kinase I (EC 2.7.1.71) AroK, 172 aa; fasta scores: opt: 331 z-score: 376.4 E(): 1.7e-13; 38.6% identity in 166 aa overlap. Contains Pfam match to entry PF01202 SKI, Shikimate kinase and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS01128 Shikimate kinase signature 3 1 10 20 [Reporter]SCO1518 (3O8)_PCR/1225 [Gene]SCO1518/1122 NC_003888 SCL2.08c, ruvB, holliday junction DNA helicase, len: 357 aa; highly similar to SW:RUVB_MYCTU (EMBL:Z77724) Mycobacterium tuberculosis holliday junction DNA helicase RuvB, 344 aa; fasta scores: opt: 1530 z-score: 1686.9 E(): 0; 68.2% identity in 343 aa overlap and to SW:RUVB_ECOLI (EMBL:X07091) Escherichia coli holliday junction DNA helicase RuvB, 336 aa;; fasta scores: opt: 1238 z-score: 1366.4 E(): 0; 54.2% identity in 330 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 10 19 [Reporter]SCO4759 (4K8)_PCR/1224 [Gene]SCO4759/1121 NC_003888 SC6G4.37, probable secreted protein, len: 264 aa; contains possible N-terminal signal sequence. Similar to down stream gene SC6G4.38 (E(): 3.9e-19, 38.7% identity in 248 aa overlap) 3 1 10 18 [Reporter]SCO6388 (5G8)_PCR/1223 [Gene]SCO6388/1120 NC_003888 SC3C8.07c, unknown, len: 119 aa 3 1 10 17 [Reporter]SCO2058 (6C8)_PCR/1222 [Gene]SCO2058/1119 NC_003888 SC4G6.27, possible transcriptional regulatory protein, len: 169 aa; similar to many e.g. TR:Q51388 (EMBL:U49666) proposed regulatory protein from the glycerol metabolism cluster in Pseudomonas aeruginosa (156 aa) fasta scores; opt: 546, z-score: 627.8, E(): 1.2e-27, (59.7% identity in 154 aa overlap). Contains possible helix-turn-helix motif (+2.63 SD) 43-64 aa. Contains possible N-terminal region signal peptide sequence 3 1 10 16 [Reporter]SCO5427 (7O4)_PCR/1221 [Gene]SCO5427/1118 NC_003888 SC6A11.03c, unknown, len: 237 aa; no significant database similarities 3 1 10 15 [Reporter]SCO5584 (8K4)_PCR/1220 [Gene]SCO5584/1117 NC_003888 SC2E1.01, glnB, nitrogen regulatory protein P-II, partial CDS, len: >54 aa; highly similar to many e.g. GLNB_AZOBR (112 aa), fasta scores; opt: 198 z-score: 381.9 E(): 4.9e-14, 50.0% identity in 54 aa overlap,SC7A1.28, partial CDS, glnB, nitrogen regulatory protein P-II, len: 92aa; similar to many eg. SW:GLNB_RHIME nitrogen regulatory protein P-II from Rhizobium meliloti (112 aa) fasta scores; opt: 402, z-score: 776.2, E(): 0, (58.7% identity in 92 aa overlap). Contains PS00496 P-II protein urydylation site and Pfam match to entry PF00543 P-II, Nitrogen regulatory protein P-II, score 116.60, E-value 7.1e-34. 3 1 10 14 [Reporter]SCO7718 (9G4)_PCR/1219 [Gene]SCO7718/1116 NC_003888 SC8D11.09c, unknown, len: 257 aa 3 1 10 13 [Reporter]SCO5124 (10C4)_PCR/1218 [Gene]SCO5124/1115 NC_003888 SC9E12.09c, hypothetical protein, len: 68 aa; similar to TR:Q9RDR5 (EMBL:AL136500) Streptomyces coelicolor hypothetical 7.7 kDa protein SC1G2.14c, 73 aa; fasta scores: opt: 217 z-score: 327.7 E(): 8.7e-11; 55.9% identity in 59 aa overlap 3 1 10 12 [Reporter]SCO2696 (10O24)_PCR/1217 [Gene]SCO2696/1114 NC_003888 SCC61A.17, possible 2-hydroxyacid-family dehydrogenase, len: 330 aa; similar to many e.g. SW:P35136 (SERA_BACSU) D-3-phosphoglycerate dehydrogenase from Bacillus subtilis (525 aa) fasta scores; opt: 519, z-score: 581.8, E(): 6e-25, 35.7% identity in 294 aa overlap. Contains Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases. 3 1 10 11 [Reporter]SCO4439 (11K24)_PCR/1216 [Gene]SCO4439/1113 NC_003888 SCD6.17c, possible D-alanyl-D-alanine carboxypeptidase, len: 832 aa; similar to TR:O54201 (EMBL:AJ001743) Streptomyces clavuligerus Pbp2 protein (penicillin binding protein PbpA), 696 aa; fasta scores: opt: 955 z-score: 744.1 E(): 0; 37.0% identity in 757 aa overlap. Contains Pfam match to entry PF00768 Peptidase_S11, D-alanyl-D-alanine carboxypeptidase and high content in alanine, aspartic acid and glycine amino acid residues. Contains degenerate repeats: 2X (G/A)GAAPDSGSG(T/A)DATXXXPAPD(T/A) KPDQEPDADSEPAPDAQAASSRDGDAD 3 1 10 10 [Reporter]SCO3509 (12G24)_PCR/1215 [Gene]SCO3509/1112 NC_003888 SCE134.10, unknown, len: 320 aa; weakly similar to SW:YD75_HAEIN hypothetical protein from Haemophilus influenzae (302 aa) fasta scores; opt: 308, z-score: 377.0, E(): 1.1e-13, (23.6% identity in 301 aa overlap) and similar to TR:Q9L1X0 (EMBL:AL138667) Streptomyces coelicolor hypothetical protein SC3D9.09, 350 aa; fasta scores: opt: 589 Z-score: 696.5 E(): 3.6e-31; 46.006% identity in 313 aa overlap 3 1 10 9 [Reporter]SCO3682 (13C24)_PCR/1214 [Gene]SCO3682/1111 NC_003888 SCH35.42c, possible delta fatty acid desaturase, len: 345 aa; similar to many (prokaryote and eukaryote) egs. TR:CAA11032 (EMBL:AJ222980) delta6-acyl-group desaturase from Physcomitrella patens (moss) (525 aa) fasta scores; opt: 499, z-score: 601.5, E(): 3.6e-26, (29.5% identity in 329 aa overlap) and TR:Q55240 (EMBL:D13780) omega 3 desaturase from Synechocystis sp. strain PCC6803 (359 aa) fasta scores; opt: 207, z-score: 253.0, E(): 9.1e-07, (22.6% identity in 283 aa overlap). Contains TTA leucine codon, possible target for bldA regulation. Contains possible hydrophobic membrane spanning regions 3 1 10 8 [Reporter]SCO5171 (14O20)_PCR/1213 [Gene]SCO5171/1110 NC_003888 SCP8.34c, unknown, len: 75 aa. Highly hydrophilic 3 1 10 7 [Reporter]SCO3624 (15K20)_PCR/1212 [Gene]SCO3624/1109 NC_003888 SCH10.02, hypothetical protein, len: 221 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 3 1 10 6 [Reporter]SCO2971 (16G20)_PCR/1211 [Gene]SCO2971/1108 NC_003888 SCE59.30c, possible secreted protein, len: 263 aa. Contains the following repeats: 2x(NGGNGNGGNGD) and 18x(PDT). Contains also possible N-terminal region signal peptide sequence 3 1 10 5 [Reporter]SCO3575 (17C20)_PCR/1210 [Gene]SCO3575/1107 NC_003888 SCH17.09c, conserved hypothetical protein, len: 155 aa; unknown function, similar to pB5 (EMBL:U67906) Trypanosoma cruzi protein only expressed in the epimastigote form of the parasite (160 aa), fasta scores; opt: 384 z-score: 444.0 E(): 2.1e-17, 47.4% identity in 152 aa overlap. Also similar to TR:O69646 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical protein (151 aa) (68.0% identity in 150 aa overlap) 3 1 10 4 [Reporter]SCP1.94 (18O16)_PCR/1209 [Gene]SCP1.94/1106 NC_003888 SCP1.94, unknown, len: 57aa; 3 1 10 3 [Reporter]SCO6095 (19K16)_PCR/1208 [Gene]SCO6095/1105 NC_003888 SCBAC1A6.19c, probable ABC transporter ATP-binding protein, len: 264 aa; similar to SW:SSUB_BACSU (EMBL:Z93102) Bacillus subtilis putative aliphatic sulfonates transport ATP-binding protein SsuB, 274 aa; fasta scores: opt: 628 z-score: 728.2 E(): 0; 46.1% identity in 217 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 1 10 2 [Reporter]SCO3052 (20C16)_PCR/1207 [Gene]SCO3052/1104 NC_003888 SCBAC19G2.07, probable UDP-glucose 6-dehydrogenase, len: 447aa: similar to many eg. SW:O54068 (UDG_RHIME) UDP-glucose 6-dehydrogenase RkpK from Rhizobium meliloti EC:1.1.1.22 (437 aa) fasta scores; opt: 1101, Z-score: 1198.1, 43.080% identity (44.780% ungapped) in 448 aa overlap. Contains Pfam match to entry PF00984 UDPG_MGDP_dh, UDP-glucose/GDP-mannose dehydrogenase family. 3 1 9 22 [Reporter]SCO2805 (1C12)_PCR/1205 [Gene]SCO2805/1103 NC_003888 2SCC13.13, hypothetical protein, len: 479 aa; similar to SW:SP15_STRGR (EMBL:M32687) Streptomyces griseus sporulation related protein ORF1590, 529 aa; fasta scores: opt: 413 z-score: 469.3 E(): 1.2e-18; 30.4% identity in 326 aa overlap. Contains 2x conserved repeat: YETHD and 3x degenerate repeat: D(T/A)QD(V/L) 3 1 9 21 [Reporter]SCO5862 (2O8)_PCR/1204 [Gene]SCO5862/1102 NC_003888 SC2E9.03, cutR, two-component regulator CutR, len: 217 aa; identical to CUTR_STRLI Q03756 transcriptional regulatory protein CutR from Streptomyces lividans (217 aa). Contains Pfam match to entry trans_reg_C PF00486, Transcriptional regulatory proteins, C terminal, score 74.36 3 1 9 20 [Reporter]SCO4659 (3K8)_PCR/1203 [Gene]SCO4659/1101 NC_003888 SCD40A.05, rpsL, 30S ribosomal protein S12, len: 123 aa; highly similar to TR:P97222 (EMBL:D83746) Streptomyces lividans ribosomal protein S12 RpsL, 123 aa; fasta scores: opt: 793 z-score: 1011.4 E(): 0; 99.2% identity in 123 aa overlap and to SW:RS12_MYCTU (EMBL:) Mycobacterium tuberculosis 30S ribosomal protein S12, RpsL, 124 asa; fasta scores: opt: 731 z-score: 933.6 E(): 0; 90.2% identity in 123 aa overlap. Contains Pfam match to entry PF00164 Ribosomal_S12, Ribosomal protein S12 and match to Prosite entry PS00055 Ribosomal protein S12 signature 3 1 9 19 [Reporter]SCO5532 (4G8)_PCR/1202 [Gene]SCO5532/1100 NC_003888 SC1C2.13, probable transcriptional regulator, len: 194 aa; simialr to many e.g. S. lividans TR:G3293540 (EMBL:AF072709) putative transcriptional regulator (192 aa), fasta scores; opt: 342 z-score: 493.9 E(): 2.9e-20, 42.0% identity in 181 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family, score 30.80, E-value 8.3e-07 3 2 8 6 [Reporter]SCO3823 (16O15)_PCR/3183 [Gene]SCO3823/2899 NC_003888 SCGD3.24c, probable quinone oxidoreductase, len: 326 aa; similar to eukaryotic quinone oxidoreductases (some putative) e.g. SW:QOR_CAVPO (EMBL:M26936) Cavia porcellus zeta-crystallin/quinone reductase (329 aa), fasta scores; opt: 565 z-score: 588.2 E(): 2e-25, 32.6% identity in 328 aa overlap. Highly similar to TR:O65423 (EMBL:AL022603) Arabidopsis thaliana putative NADPH quinone oxidoreductase (325 aa) (57.2% identity in 325 aa overlap). Similar to SCE68.12c, S.coelicolor possible dehydrogenase (318 aa) (31.1% identity in 325 aa overlap). Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases 3 2 8 5 [Reporter]SCO3397 (17K15)_PCR/3182 [Gene]SCO3397/2898 NC_003888 SCE9.04, possible integral membrane lysyl-tRNA synthetase, len: 601 aa; unknown function, similar to hypothetical proteins e.g. N-terminal half of SW:P94974 (SYK2_MYCTU) Mycobacterium tuberculosis probable lysyl-tRNA synthetase (1172 aa), fasta scores; opt: 1245 z-score: 1445.4 E(): 0, 39.9% identity in 604 aa overlap and TR:O69916 (EMBL:AL023861) S.coelicolor putative integral membrane protein (589 aa) (45.8% identity in 539 aa overlap). Contains hydrophobic, possible membrane-spanning regions 3 2 8 4 [Reporter]SCO5144 (18G15)_PCR/3181 [Gene]SCO5144/2897 NC_003888 SCP8.07c, probable acyl CoA isomerase, len: 267 aa; highly similar to TR:AAF73478 (EMBL:AF268489) Streptomyces collinus 2-cyclohexenylcarbonyl CoA isomerase ChcB, 269 aa; fasta scores: opt: 1476 z-score: 1623.5 E(): 0; 87.6% identity in 266 aa overlap and to SW:CRT_CLOAB (EMBL:U17110) Clostridium acetobutylicum 3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) Crt, 261 aa; fasta scores: opt: 426 z-score: 476.6 E(): 4.4e-19; 32.4% identity in 259 aa overlap. Contains Pfam match to entry PF00378 ECH, Enoyl-CoA hydratase/isomerase family and match to Prosite entry PS00166 Enoyl-CoA hydratase/isomerase signature 3 2 8 3 [Reporter]SCO4045 (19C15)_PCR/3180 [Gene]SCO4045/2896 NC_003888 2SCD60.11c, possible integral membrane protein, len: 226 aa; similar to TR:O06141 (EMBL:Z95554) Mycobacterium tuberculosis hypothetical 20.4 kDa protein MTCY01B2.16c, 195 aa; fasta scores: opt: 628 z-score: 680.7 E(): 2.4e-30; 50.8% identity in 191 aa overlap. Contains possible hydrophobic membrane spanning regions 3 2 8 2 [Reporter]SCO0372 (20K11)_PCR/3179 [Gene]SCO0372/2895 NC_003888 SCF41.31c, possible secreted protein, len: 1024 aa. Contains a possible N-terminal signal sequence. 3 2 7 22 [Reporter]SCO4223 (1K7)_PCR/3177 [Gene]SCO4223/2894 NC_003888 2SCD46.37c, possible AraC-family transcriptional regulator, len: 295aa; similar to many eg. SW:P22539 (SOXS_ECOLI) regulatory protein SoxS from Escherichia coli (106 aa) fasta scores; opt: 186, z-score: 223.7, E(): 5.4e-05, 28.4% identity in 95 aa overlap and TR:P96662 (EMBL:AB001488) probable hth_arac_family of transcriptional regulator from Bacillus subtilis (290 aa) fasta scores; opt: 726, z-score: 825.6, E(): 0, 37.7% identity in 289 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family and Prosite match to PS00041 Bacterial regulatory proteins, araC family signature. 3 2 7 21 [Reporter]SCO2389 (2G7)_PCR/3176 [Gene]SCO2389/2893 NC_003888 SC4A7.17, acpP, acyl carrier protein, len: 82 aa; identical to previously sequenced TR:P72393 (EMBL:X86475) Streptomyces coelicolor acyl carrier protein AcpP, 82 aa and similar to SW:ACP_MYXXA Myxcoccus xanthus acyl carrier protein AcpP, 78 aa; fasta scores: opt: 306 z-score: 396.9 E(): 1e-14; 62.7% identity in 75 aa overlap. Contains Pfam match to entry PF00550 pp-binding, Phosphopantetheine attachment site 3 2 7 20 [Reporter]SCO3084 (3C7)_PCR/3175 [Gene]SCO3084/2892 NC_003888 SCE25.25c, pobA, p-hydroxybenzoate hydroxylase, len: 389 aa; highly similar to SW:PHHY_PSEAE (EMBL:M23173) Pseudomonas aeruginosa p-hydroxybenzoate hydroxylase (EC 1.14.13.2) (4-hydroxybenzoate 3-monooxygenase) (PhbH) PobA, 394 aa; fasta scores: opt: 1334 z-score: 1559.4 E(): 0; 50.9% identity in 389 aa overlap and to TR:Q06519 (EMBL:L13747) Pseudomonas fluorescens p-hydroxybenzoate hydroxylase (EC 1.14.13.2) (4-hydroxybenzoate 3-monooxygenase), 397 aa; fasta scores: opt: 1395 z-score: 1630.5 E(): 0; 54.5% identity in 389 aa overlap. Contains Pfam matches to entries PF01494 FAD_binding_3, FAD binding domainand PF01360 Monooxygenase, Monooxygenase 3 2 7 19 [Reporter]SCO6512 (4O3)_PCR/3174 [Gene]SCO6512/2891 NC_003888 SC1E6.21, probable ABC transporter ATP-binding protein, len: 539 aa; similar to many e.g. TLRC_STRFR tylosin resistance ATP-binding protein TLRC (548 aa), fasta scores; opt: 449 z-score: 533.0 E(): 2.1e-22, 33.0% identity in 542 aa overlap. Contains 2x PS00017 ATP /GTP-binding site motif A (P-loop) and 2x Pfam match to entry PF00005 ABC_tran, ABC transporters, scores 125.80, E-value 8.1e-34, and 69.10, E-value 9.1e-17 3 2 7 18 [Reporter]SCO5887 (5K3)_PCR/3173 [Gene]SCO5887/2890 NC_003888 SC3F7.07c, redQ, probable acyl carrier protein, len; 81 aa; similar to several eg. ACP_SYNY3 P20804 acyl carrier protein (acp) from Synechocystis (77 aa),fasta scores; opt: 153 z-score: 256.6 E(): 4.2e-07, 34.7% identity in 72 aa overlap 3 2 7 17 [Reporter]SCO6640 (6G3)_PCR/3172 [Gene]SCO6640/2889 NC_003888 SC4G2.14, probable ATP-dependent helicase, len: 731 aa; similar to many predicted helicases e.g. C-terminuis of LHR_ECOLI probable ATP-dependent helicase LHR (1538 aa), fasta scores; opt: 332 z-score: 570.0 E(): 1.7e-24, 29.3% identity in 638 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop). Contains Pfam match to entry PF00271 helicase_C, Helicases conserved C-terminal domain 3 2 7 16 [Reporter]SCO0312 (7C3)_PCR/3171 [Gene]SCO0312/2888 NC_003888 SC5G9.21, probable acyl-CoA dehydrogenase, len: 389 aa; similar to both bacterial and eukaryotic acyl-CoA dehydrogenases e.g. SW:ACDS_HUMAN (EMBL:M26393), AcaDS, Homo sapiens mitochondrial acyl-CoA dehydrogenase short-chain specific precursor (412 aa), fasta scores; opt: 653 z-score: 771.4 E(): 0, 34.8% identity in 376 aa overlap. Also similar to SCI30A.22C (EMBL:AL096811) Streptomyces coelicolor probable acyl-CoA dehydrogenase. Contains 2 Pfam matches to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase 3 2 7 15 [Reporter]SCO2130 (7O23)_PCR/3170 [Gene]SCO2130/2887 NC_003888 SC6G10.03c, hypothetical protein, len: 265 aa; weak similarity to many eg. TR:P95860 (EMBL:Y08256) Sulfolobus solfataricus hypothetical protein ORF CO6013 (309 aa) fasta scores; opt: 152, z-score: 186.9, E(): 0.0041, (24.8% identity in 270 aa overlap). 3 2 7 14 [Reporter]SCO2417 (8K23)_PCR/3169 [Gene]SCO2417/2886 NC_003888 SC8A2.05c, possible secreted protein, len: 351 aa; similar to TR:O53969 (EMBL:AL022073) Mycobacterium tuberculosis hypothetical 43.7 kDa protein MTV051.06, 410 aa; fasta scores: opt: 683 z-score: 769.5 E(): 0; 33.4% identity in 368 aa overlap. Contains Pfam match to entry PF02470 mce. Also contains possible N-terminal region signal peptide sequence 3 2 7 13 [Reporter]SCO6069 (9G23)_PCR/3168 [Gene]SCO6069/2885 NC_003888 SC9B1.16c, cvnA6, possible large secreted protein, len: 877aa; similar to two neighbouring hypothetical proteins from Streptomyces coelicolor TR:O86525 (EMBL:AL031124) (1329 aa) fasta scores; opt: 1056, z-score: 919.5, E(): 0, (30.4% identity in 891 aa overlap) TR:O86522 (EMBL:AL031124) (1111 aa) fasta scores; opt: 804, z-score: 701.8, E(): 9e-32, (28.4% identity in 849 aa overlap). Contains possible N-terminal signal sequence. 3 2 7 12 [Reporter]SCO2722 (10C23)_PCR/3167 [Gene]SCO2722/2884 NC_003888 SCC46.07c, possible integral membrane protein, len: 299 aa. Contains possible hydrophobic membrane spanning regions 3 2 7 11 [Reporter]SCO4626 (11O19)_PCR/3166 [Gene]SCO4626/2883 NC_003888 SCD39.26c, unknown, len: 177 aa 3 2 7 10 [Reporter]SCO2927 (12K19)_PCR/3165 [Gene]SCO2927/2882 NC_003888 SCE19A.27c, probable 4-hydroxyphenylpyruvate dioxygenase, len: 381 aa; highly similar to many e.g. SW:HPPD_STRAW (EMBL:U11864) Streptomyces avermitilis 4-hydroxyphenylpyruvate dioxygenase (380 aa), fasta scores; opt: 2347 z-score: 2634.5 E(): 0, 91.9% identity in 381 aa overlap. Similar to TR:Q9Z4X7 (EMBL:AL035640) S. coelicolor probable 4-hydroxyphenylpyruvic acid dioxygenase (325 aa) (36.4% identity in 330 aa overlap) 3 2 7 9 [Reporter]SCO0339 (13G19)_PCR/3164 [Gene]SCO0339/2881 NC_003888 SCF12.18, possible dehydrogenase, len: 189 aa; similar to a region from many proposed dehydrogenases e.g. SW:SERA_METJA D-3-phosphoglycerate dehydrogenase from Methanococcus jannaschii (524 aa) fasta scores; opt: 415, z-score: 508.1, E(): 6e-21, 40.8% identity in 179 aa overlap. Contains Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases 3 2 7 8 [Reporter]SCO1812 (14C19)_PCR/3163 [Gene]SCO1812/2880 NC_003888 SCI28.06c, possible integral membrane transporter, len: 421aa; similar to SW:CYNX_ECOLI cyanate transport protein from Escherichia coli (384 aa) fasta scores; opt: 328, z-score: 364.0, E(): 6.1e-13, (28.0% identity in 386 aa overlap). Also similar to TR:O68332 (EMBL:AF033674) hypothetical protein from Pseudomonas marginalis pv. alfalfae (420 aa) fasta scores; opt: 521, z-score: 574.2, E(): 1.2e-24, (37.7% identity in 385 aa overlap). Contains possible membrane spanning hydrophobic regions. 3 2 7 7 [Reporter]SCO1559 (15O15)_PCR/3162 [Gene]SCO1559/2879 NC_003888 SCL11.15c, probable ABC transporter ATP-binding protein, len: 368 aa; similar to SW:ABC_ECOLI (EMBL:L08626) Escherichia coli ATP-binding protein Abc, 343 aa; fasta scores: opt: 909 z-score: 1006.2 E(): 0; 49.1% identity in 346 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporte and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 2 7 6 [Reporter]SCO1236 (16K15)_PCR/3161 [Gene]SCO1236/2878 NC_003888 2SCG1.11c, ureA, urease gamma subunit, len: 100 aa; similar to SW:URE3_MYCTU (EMBL:L41141) Mycobacterium tuberculosis urease gamma subunit (EC 3.5.1.5) UreA, 100 aa; fasta scores: opt: 498 z-score: 638.4 E(): 4.3e-28; 71.7% identity in 99 aa overlap. Contains Pfam match to entry PF00547 urease_gamma, Urease, gamma subunit 3 2 7 5 [Reporter]SCO0116 (17G15)_PCR/3160 [Gene]SCO0116/2877 NC_003888 SCJ11.45c, possible tetR-family transcriptional regulator, len: 188 aa; N-terminal region similar to that of others e.g. SW:ACRR_ECOLI potential AcrAB operon repressor (215 aa) fasta scores; opt: 148, z-score: 188.9, E(): 0.0037, (39.3% identity in 61 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Also contains possible helix-turn-helix motif 32-53aa (+3.34 SD). 3 2 7 4 [Reporter]SCO1165 (18C15)_PCR/3159 [Gene]SCO1165/2876 NC_003888 SCG8A.19, possible integral membrane protein, len: 305aa; Contains possible membrane-spanning hydrophobic regions. 3 2 7 3 [Reporter]SCP1.81 (19O11)_PCR/3158 [Gene]SCP1.81/2875 NC_003888 SCP1.81, unknown, len: 140aa; similar to TR:Q54403 (EMBL:Z11519) hypothetical protein from transposable element, Tn4811 of Streptomyces lividans 66 (138 aa) fasta scores; opt: 350, z-score: 452.4, E(): 9.8e-18, 43.0% identity in 135 aa overlap. Also similar to SCP1.39c/SCP1.315 (previously sequenced as TR:Q9L8H2 (EMBL:AF204735)) (139 aa) fasta scores; opt: 348, z-score: 449.9, E(): 1.4e-17, 43.4% identity in 136 aa overlap. 3 2 7 2 [Reporter]SCO4884 (20G11)_PCR/3157 [Gene]SCO4884/2874 NC_003888 2SCK8.10, probable lipoprotein, len: 347 aa; similar to SW:YUFN_BACSU (EMBL:Z93937) Bacillus subtilis hypothetical lipoprotein precursor YufN, 350 aa; fasta scores: opt: 690 z-score: 727.5 E(): 0; 37.4% identity in 348 aa overlap, to TR:CAC14304 (EMBL:AJ276209) Clostridium sticklandii putative substrate binding lipoprotein precursor of an ABC transporter BmpA, 349 aa; fasta scores: opt: 557 z-score: 589.0 E(): 3.1e-25; 36.6% identity in 352 aa overlap and to Streptomyces coelicolor probable lipoprotein 2SCK8.11, 348 aa; fasta scores: opt: 1727 z-score: 1625.1 E(): 0; 78.4% identity in 347 aa overlap. Contains Pfam match to entry PF02608 Bmp and properly positioned match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 3 2 6 22 [Reporter]SCO5245 (1G7)_PCR/3155 [Gene]SCO5245/2873 NC_003888 2SC7G11.07c, hypothetical protein, len: 86 aa; identical to previously sequenced TR:Q9R3I9 (EMBL:AJ249450) Streptomyces coelicolor A3(2) hypothetical 9.6 kDa protein UshY, 86 aa. Contains Pfam match to entry PF02148 zf-UBP, Zn-finger in ubiquitin-hydrolases and other proteins 3 2 6 21 [Reporter]SCO2774 (2C7)_PCR/3154 [Gene]SCO2774/2872 NC_003888 SCC105.05c, acdH2, probable acyl-CoA dehydrogenase, len: 385 aa; similar to TR:Q9X7Y2 (EMBL:AL049485) Streptomyces coelicolor probable acyl-CoA dehydrogenase SC6A5.36, 382 aa; fasta scores: opt: 1170 z-score: 1351.4 E(): 0; 47.9% identity in 382 aa overlap. Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase and match to Prosite entry PS00073 Acyl-CoA dehydrogenases signature 2 3 2 6 20 [Reporter]SCO4734 (3O3)_PCR/3153 [Gene]SCO4734/2871 NC_003888 SC6G4.12, rplM, 50S ribosomal protein L13, len: 147 aa; identical to RL13_STRCO 50S ribosomal protein L13 (147 aa) and highly similar to many e.g. RL13_ECOLI (142 aa), fa sta scores; opt: 557 z-score: 970.2 E(): 0, 53.5% identity in 142 aa overlap. Contains PS00783 Ribosomal protein L13 s ignature and Pfam match to entry PF00572 L13, Ribosomal pro tein L13, score 252.90, E-value 4.4e-72 3 2 6 19 [Reporter]SCO6503 (4K3)_PCR/3152 [Gene]SCO6503/2870 NC_003888 SC1E6.12, unknown, len: 239 aa; contains highly acidic repetitive stretch between aa 120-180 and highly basic repetitive stretch between aa 180-239 3 2 6 18 [Reporter]SCO6351 (5G3)_PCR/3151 [Gene]SCO6351/2869 NC_003888 SC3A7.19, unknown, len: 232 aa; 3 2 6 17 [Reporter]SCO6634 (6C3)_PCR/3150 [Gene]SCO6634/2868 NC_003888 SC4G2.08, unknown, len: 62 aa 3 2 6 16 [Reporter]SCO4848 (6O23)_PCR/3149 [Gene]SCO4848/2867 NC_003888 SC5G8.16, possible integral membrane protein, len: 79aa; contains possible membrane-spanning hydrophobic regions. Position of translational start uncertain, possible alternative start at 13619. 3 2 6 15 [Reporter]SCO6971 (7K23)_PCR/3148 [Gene]SCO6971/2866 NC_003888 SC6F7.24c, possible methylase, len: 257 aa. Highly similar to Pseudomonas denitrificans SW:COBF_PSEDE(EMBL:M59301) cobalamin biosynthesis protein, CobF (261 aa), fasta scores opt: 869 z-score: 1009.4 E():0 53.6% identity in 252 aa overlap. Contains a Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases. 3 2 6 14 [Reporter]SCO5945 (8G23)_PCR/3147 [Gene]SCO5945/2865 NC_003888 SC7H1.15, probable transferase, len: 606 aa; similar to TR:Q54353 (EMBL:X64651) S.lincolnensis lmbA gene for lincomycin condensing protein (601 aa), fasta scores; opt: 921 z-score: 1470.0 E(): 0, 45.8% identity in 600 aa overlap and to other gamma-glutamyl transferases 3 2 6 13 [Reporter]SCO6065 (9C23)_PCR/3146 [Gene]SCO6065/2864 NC_003888 SC9B1.12, probable secreted substrate-binding protein, len: 320aa; similar to many eg. TR:O69725 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (315 aa) fasta scores; opt: 574, z-score: 637.7, E(): 3.4e-28, (35.8% identity in 313 aa overlap) and SW:PROX_BACSU glycine betaine/L-proline binding protein from Bacillus subtilis (313 aa) fasta scores; opt: 136, z-score: 157.8, E(): 0.18, (25.7% identity in 311 aa overlap). Contains possible N-terminal signal sequence. 3 2 6 12 [Reporter]SCO2737 (10O19)_PCR/3145 [Gene]SCO2737/2863 NC_003888 SCC57A.08c, possible deoxyribonuclease, len: 753 aa. Similar to several including: Chlamydia trachomatis TR:O84036(EMBL:AE001278) exodeoxyribonuclease V, alpha subunit (746 aa), fasta scores opt:1007 z-score: 1095.8 E():0 36.8% identity in 740 aa overlap and to the C-terminus of Escherichia coli SW:EX5A_ECOLI(EMBL:X04582) exodeoxyribonuclease V (EC 3.1.11.5) RecD (608 aa), fasta scores opt: 225 z-score: 245.0 E(): 2.9e-06 29.3% identity in 460 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 3 2 6 11 [Reporter]SCO4619 (11K19)_PCR/3144 [Gene]SCO4619/2862 NC_003888 SCD39.19c, possible integral membrane protein, len: 19c aa. Contains possible hydrophobic membrane spanning regions 3 2 6 10 [Reporter]SCO4300 (12G19)_PCR/3143 [Gene]SCO4300/2861 NC_003888 SCD95A.33c, hypothetical protein, len: 89 aa; similar to TR:CAB69735 (EMBL:AL137166) Streptomyces coelicolor hypothetical 10.2 kD protein SCC121.17, 96 aa; fasta scores: opt: 251 z-score: 338.4 E(): 2.2e-11; 51.1% identity in 94 aa overlap 3 2 6 9 [Reporter]SCO0168 (13C19)_PCR/3142 [Gene]SCO0168/2860 NC_003888 SCJ1.17, possible regulator, len: 190 aa, similar to Mycobacterium tuberculosis TR:O69644 (EMBL; AL022121) putative transcriptional regulator MTV025.024 (224 aa), fasta scores opt: 199 z-score: 254.8 E(): 7.8e-07 31.5% identity in 124 aa overlap. Contains a Pfam match to entry PF00027 cNMP_binding, Cyclic nucleotide-binding domain. 3 2 6 8 [Reporter]SCO1808 (14O15)_PCR/3141 [Gene]SCO1808/2859 NC_003888 SCI28.02, possible phosphoserine phosphatase, len: 410 aa; similar to many e.g. SW:SERB_ECOLI phosphoserine phosphatase from Escherichia coli (322 aa) fasta scores; opt: 621, z-score: 674.7, E(): 3e-30, (49.8% identity in 219 aa overlap). Also similar to TR:O53289 (EMBL:AL021287) hypothetical protein from Mycobacterium tuberculosis (409 aa) fasta scores; opt: 1209, z-score: 1304.1, E(): 0, (51.8% identity in 400 aa overlap). 3 2 6 7 [Reporter]SCO3679 (15K15)_PCR/3140 [Gene]SCO3679/2858 NC_003888 SCH35.45c, unknown, len: 634 aa; similar to TR:CAA22732 (EMBL:AL035161) hypothetical protein from Streptomyces coelicolor (860 aa) fasta scores; opt: 551, z-score: 565.3, E(): 3.7e-24, (29.7% identity in 683 aa overlap). Contains Pfam match to entry PF00989 PAS, PAS domain. 3 2 6 6 [Reporter]SCO0548 (16G15)_PCR/3139 [Gene]SCO0548/2857 NC_003888 SCF11.28c, fabB2, possible 3-oxoacyl-(acyl-carrier-protein) synthase, len: 428 aa; similar to TR:Q54208 (EMBL:L43074) Streptomyces glaucescens fabB, 423 aa; fasta scores: opt: 1375 z-score: 1459.4 E(): 0; 52.1% identity in 420 aa overlap. Contains Pfam match to entry PF00109 ketoacyl-synt, beta-ketoacyl synthase 3 2 6 5 [Reporter]SCO6661 (17C15)_PCR/3138 [Gene]SCO6661/2856 NC_003888 SC5A7.11c, zwf, probable glucose-6-phosphate 1-dehydrogenase, len: 592 aa; similar to many, e.g. G6PD_ECOLI glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) (491 aa), fasta scores; opt: 1145 z-score: 1213.9 E(): 0, 43.1% identity in 487 aa overlap. Alternative start site at aa 83 predicted by database similarity. Contains PS00069 Glucose-6-phosphate dehydrogenase active site and Pfam match to entry PF00479 G6PD, Glucose-6-phosphate dehydrogenase, score 730.30, E-value 1.1e-236 3 2 6 4 [Reporter]SCO1709 (18O11)_PCR/3137 [Gene]SCO1709/2855 NC_003888 SCI30A.30c, possible integral membrane transport protein, len: 463aa; weak similarity to many eg. TR:Q45632 (EMBL:L13418) maltose permease from Bacillus stearothermophilus (394 aa) fasta scores; opt: 220, z-score: 200.9, E(): 0.00074, (28.7% identity in 363 aa overlap). Contains a 13x9bp degenerate repeat G/S)GA in the C-terminal region similar to one found in the N-terminal region of TR:Q94673 (EMBL:U72957) merozoite surface protein-2 of Plasmodium falciparum (176 aa) fasta scores; opt: 237, z-score: 219.6, E(): 6.7e-05, (45.4% identity in 97 aa overlap). Also contains possible membrane spanning hydrophobic regions. 3 2 6 3 [Reporter]SCP1.168 (19K11)_PCR/3136 [Gene]SCP1.168/2854 NC_003888 SCP1.168, unknown, len: 292aa; similar to TR:Q9L0R9 (EMBL:AL160312) hypothetical protein from Streptomyces coelicolor (293 aa) fasta scores; opt: 262, z-score: 304.0, E(): 1.8e-09, 30.3% identity in 300 aa overlap. 3 2 6 2 [Reporter]SCO1296 (20C11)_PCR/3135 [Gene]SCO1296/2853 NC_003888 SCBAC36F5.07, conserved hypothetical protein, len: 562 aa; similar to TR:Q9A3Z7 (EMBL:AE005968) Caulobacter crescentus hypothetical protein CC3053, 597 aa; fasta scores: opt: 331 Z-score: 361.3 bits: 76.8 E(): 1.6e-12; 28.308% identity in 597 aa overlap 3 2 5 22 [Reporter]SCO5395 (1C7)_PCR/3133 [Gene]SCO5395/2852 NC_003888 2SC6G5.39c, possible ABC transporter ATP-binding subunit, len: 346aa; similar to many eg. TR:Q9S6T6 (EMBL:AL035654) putative ABC transporter ATP-binding protein from Streptomyces coelicolor (317 aa) fasta scores; opt: 1180, z-score: 1223.8, E(): 0, 58.5% identity in 316 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 2 5 21 [Reporter]SCO5206 (2O3)_PCR/3132 [Gene]SCO5206/2851 NC_003888 SC7E4.03c, catR, hydrogen peroxide sensitive repressor, len: 138 aa; identical to previously sequenced TR:Q9RN70 (EMBL:AF186372) Streptomyces coelicolor hydrogen peroxide sensitive repressor CatR, 138 aa. Contains Pfam match to entry PF01475 FUR, Ferric uptake regulator family 3 2 5 20 [Reporter]SCO4717 (3K3)_PCR/3131 [Gene]SCO4717/2850 NC_003888 SCD31.42, rplF, 50S ribosomal protein L6, len: 179 aa; identical to previosuly sequenced SW:RL6_STRCO (EMBL:X83011) Streptomyces coelicolor 50S ribosomal protein L6 RplF, 179 aa. Contains Pfam match to entry PF00347 Ribosomal_L6, Ribosomal protein L6 and match to Prosite entry PS00525 Ribosomal protein L6 signature 1 3 2 5 19 [Reporter]SCO6906 (4G3)_PCR/3130 [Gene]SCO6906/2849 NC_003888 SC1B2.12c, unknown, len: 529 aa. Contains a 4xRPPHSPGPTPPRTKD repeat towards the C-terminal. Note low G+C content of this CDS (57.23 %) 3 2 5 18 [Reporter]SCO6347 (5C3)_PCR/3129 [Gene]SCO6347/2848 NC_003888 SC3A7.15, probable beta-galactosidase, len: 595; similar to many e.g. TR:O31341 (EMBL:D88750) beta-galactosidase (EC 3.2.1.23) (lactase) from Bacillus circulans (586 aa), fasta scores; opt: 1648 z-score: 1500.8 E(): 0, 43.0% identity in 584 aa overlap., Contains PS01182 Glycosyl hydrolases family 35 putative active site 3 2 5 17 [Reporter]SCO7071 (5O23)_PCR/3128 [Gene]SCO7071/2847 NC_003888 SC4G1.37c, conserved hypothetical protein, len: 130 aa; similar to TR:Q9RZR3 (EMBL:AE001826) Deinococcus radiodurans conserved hypothetical protein DRB0052, 133 aa; fasta scores: opt: 250 z-score: 278.2 E(): 5e-08; 42.2% identity in 135 aa overlap 3 2 5 16 [Reporter]SCO4844 (6K23)_PCR/3127 [Gene]SCO4844/2846 NC_003888 SC5G8.12, possible integral membrane protein, len: 322aa; similar to TR:O53583 (EMBL:AL022076) hypothetical protein from Mycobacterium tuberculosis (302 aa) fasta scores; opt: 937, z-score: 1058.4, E(): 0, 51.4% identity in 292 aa overlap. Contains possible membrane-spanning hydrophobic regions 3 2 5 15 [Reporter]SCO7442 (7G23)_PCR/3126 [Gene]SCO7442/2845 NC_003888 SC6D11.38, unknown, len: 154 aa. Rich in the amino acid Ala. 3 2 5 14 [Reporter]SCO5941 (8C23)_PCR/3125 [Gene]SCO5941/2844 NC_003888 SC7H1.11, probable aminotransferase, len: 382; similar to many eg. AAT_BACST Q59228 aspartate aminotransferase (ec 2.6.1.1 (393 aa), fasta scores; opt: 683 z-score: 686.1 E(): 5.1e-31, 35.0% identity in 374 aa overlap. Contains Pfam match to entry aminotran_1 PF00155, Aminotransferases class-I, score 177.68 3 2 5 13 [Reporter]SCO6450 (9O19)_PCR/3124 [Gene]SCO6450/2843 NC_003888 SC9B5.17, conserved hypothetical protein, len: 169 aa; similar to several hypothetical proteins eg. TR:Q44020 (EMBL:L36817) hypothetical protein from Alcaligenes eutrophus (173 aa) fasta scores; opt: 482, z-score: 757.7, E(): 0, (50.6% identity in 174 aa overlap). Also similar to TR:O75377 (EMBL:AF044286) histone macro 2A1.1 from Homo sapiens (369 aa) fasta scores; opt: 256, z-score: 321.4, E(): 1.3e-10, (29.4% identity in 170 aa overlap) 3 2 5 12 [Reporter]SCO2732 (10K19)_PCR/3123 [Gene]SCO2732/2842 NC_003888 SCC57A.03c, possible integral-membrane protein, len: 199 aa. Contains possible membrane spanning hydrophobic domains. 3 2 5 11 [Reporter]SCO4590 (11G19)_PCR/3122 [Gene]SCO4590/2841 NC_003888 SCD20.08c, hypothetical protein, len: 156 aa; similar to TR:CAB86125 (EMBL:AL163003) Streptomyces coelicolor hypothetical 16.0 kDa protein SCC24.31c, 142 aa; fasta scores: opt: 205 z-score: 265.0 E(): 2.7e-07; 34.0% identity in 156 aa overlap 3 2 5 10 [Reporter]SCO4293 (12C19)_PCR/3121 [Gene]SCO4293/2840 NC_003888 SCD95A.26, probable threonine synthase, len: 428 aa; similar to TR:P73711 (EMBL:D90908) Synechocystis sp. threonine synthase ThrC, 433 aa; fasta scores: opt: 1467 z-score: 1612.7 E(): 0; 55.0% identity in 422 aa overlap. Contains Pfam match to entry PF00291 S_T_dehydratase, Pyridoxal-phosphate dependent enzyme 3 2 5 9 [Reporter]SCO0284 (13O15)_PCR/3120 [Gene]SCO0284/2839 NC_003888 SCF85.12, possible secreted alpha-galactosidase, len: 680 aa. Highly similar to many Eukaryotic alpha-galactosidases over the first 415 aa, e.g. Coffea arabica (Coffee) alpha-galactosidase precursor (EC 3.2.1.22) (melibiase) (378 aa),opt: 1323 z-score: 1416.9 E(): 0; 54.5% identity in 354 aa overlap, in addition to Saccharopolyspora erythraea (Streptomyces erythraeus)alpha galactosidase precursor MelA (428 aa),fasta scores opt: 623 z-score: 668.6 E(): 6.9e-30 50.6% identity in 427 aa overlap. Contains a PS00512 Alpha-galactosidase signature, a PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site and a possible N-terminal signal sequence 3 2 5 8 [Reporter]SCO1051 (14K15)_PCR/3119 [Gene]SCO1051/2838 NC_003888 SCG20A.31, possible integral membrane protein, len: 357 aa; similar to TR:Q9S290 (EMBL:AL096849) Streptomyces coelicolor putative membrane protein SCI11.29, 354 aa; fasta scores: opt: 422 z-score: 473.8 E(): 6.5e-19; 30.5% identity in 357 aa overlap 3 2 5 7 [Reporter]SCO1730 (15G15)_PCR/3118 [Gene]SCO1730/2837 NC_003888 SCI11.19, hypothetical protein, len: 166 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Overlaps the upstream CDS by 6 codons 3 2 5 6 [Reporter]SCO3364 (16C15)_PCR/3117 [Gene]SCO3364/2836 NC_003888 SCE94.15, unknown, len: 150aa; 3 2 5 5 [Reporter]SCO2945 (17O11)_PCR/3116 [Gene]SCO2945/2835 NC_003888 SCE59.04c, possible sugar transporter membrane protein, len: 316 aa; similar to TR:CAB59589 (EMBL:AL132662) Streptomyces coelicolor putative sugar transporter inner membrane protein SCF11.12, 302 aa; fasta scores: and to TR:CAB55533 (EMBL:AL117322) Streptomyces coelicolor putative binding-protein-dependent transport protein SCF1.13, 317 aa; fasta scores: opt: 527 z-score: 611.6 E(): 1.2e-26; 33.1% identity in 302 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Contains also possible hydrophobic membrane spanning regions 3 2 5 4 [Reporter]SCO1055 (18K11)_PCR/3115 [Gene]SCO1055/2834 NC_003888 SCG22.01c, probable hydrolase (fragment), len: >346 aa; similar to N-terminal part of TR:P96793 (EMBL:U89276) Lactobacillus pentosus alpha-xylosidase XylQ, 762 aa; fasta scores: opt: 1174 z-score: 1386.0 E(): 0; 50.0% identity in 346 aa overlap. Contains Pfam match to entry PF01055 Glyco_hydro_31, Glycosyl hydrolases family 31,SCG20A.35c, probable sugar hydrolase (fragment), len: >443 aa; similar to TR:P96793 (EMBL:U89276) Lactobacillus pentosus alpha-xylosidase XylQ, 762 aa; fasta scores: opt: 1319 z-score: 1518.7 E(): 0; 47.1% identity in 437 aa overlap. Contains Pfam match to entry PF01055 Glyco_hydro_31, Glycosyl hydrolases family 31 3 2 5 3 [Reporter]SCO4047 (19G11)_PCR/3114 [Gene]SCO4047/2833 NC_003888 2SCD60.13c, conserved hypothetical protein, len: 197 aa; similar to TR:O06554 (EMBL:Z95584) Mycobacterium tuberculosis hypothetical 20.8 kDa protein MTCI65.23, 197 aa; fasta scores: opt: 741 z-score: 862.4 E(): 0; 60.6% identity in 188 aa overlap 3 2 4 22 [Reporter]SCO4193 (1O3)_PCR/3111 [Gene]SCO4193/2832 NC_003888 2SCD46.07, possible ATP/GTP-binding membrane protein, len: 333aa; N-terminal region weakly similar to SW:P07682 (LEPA_ECOLI) GTP-binding protein LepA from Escherichia coli (599 aa) fasta scores; opt: 142, z-score: 162.3, E(): 0.15, 22.9% identity in 341 aa overlap. Contains possible membrane-spanning hydrophobic regions Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 2 4 21 [Reporter]SCO3831 (2K3)_PCR/3110 [Gene]SCO3831/2831 NC_003888 SCGD3.32c, bkdA2, probable branched-chain alpha keto acid dehydrogenase E1 alpha subunit, partial CDS, len: >179 aa; similar to E1 alpha subunits from pyruvate dehydrogenase complexes and branched-chain alpha keto acid dehydrogenases e.g. TR:Q53592 (EMBL:U17169), BkdA, Streptomyces avermitilis branched-chain alpha-keto acid dehydrogenase E1-alpha subunit (381 aa), fasta scores; opt: 987 z-score: 1131.6 E(): 0, 90.3% identity in 165 aa overlap and SW:ODPA_ACHLA (EMBL:M81753), PdhA, Acholeplasma laidlawii pyruvate dehydrogenase E1 component, alpha subunit (345 aa) (34.6% identity in 162 aa overlap). Similar to SCGD3.18c, probable branched-chain alpha keto acid E1 alpha subunit (417 aa) (38.8% identity in 165 aa overlap), in a duplication of this region downstream. Contains probable coiled-coil from 148 to 176 (29 residues) (Max score: 1.437, probability 0.84),SCH69.01c, bkdA2, E1-alpha branched-chain alpha keto acid dehydrogenase, len: >261aa; very similar to the first 261 aa of TR:Q53592 (EMBL:U17169) BkdA, E1-alpha branched-chain alpha keto acid dehydrogenase from Streptomyces avermitilis (381 aa) fasta scores; opt: 1602, z-score: 1786.0, E(): 0, (90.8% identity in 261 aa overlap). Contains Pfam match to entry PF00676 E1_dehydrog, Dehydrogenase E1 component. 3 2 4 19 [Reporter]SCO6902 (4C3)_PCR/3108 [Gene]SCO6902/2830 NC_003888 SC1B2.08, unknown, len: 162 aa. 3 2 4 18 [Reporter]SCO7077 (4O23)_PCR/3107 [Gene]SCO7077/2829 NC_003888 SC3A4.03c, putative membrane protein len: 716 aa. Similar to multiple putative membrane proteins e.g. Streptomyces coelicolor SW:MMLA_STRCO(EMBL:M64683) putative membrane protein ActII-3 in the actinorhodin biosynthetic region (711 aa) fasta scores, opt: 1747 z-score: 1956.0 E():0 43.0% identity in 702 aa overlap. Contains probable membrane-spanning domains. 3 2 4 17 [Reporter]SCO7067 (5K23)_PCR/3106 [Gene]SCO7067/2828 NC_003888 SC4G1.33, hypothetical protein, len: 183 aa; similar to TR:Q9X399 (EMBL:AF065442) Vibrio cholerae hypothetical 20.4 kDa protein AphA, 179 aa; fasta scores: opt: 392 z-score: 485.9 E(): 1.3e-19; 35.4% identity in 178 aa overlap 3 2 4 16 [Reporter]SCO6728 (6G23)_PCR/3105 [Gene]SCO6728/2827 NC_003888 SC5F2A.11, putative membrane protein, len: 48 aa. Contains possible hydrophobic membrane spanning region 3 2 4 15 [Reporter]SCO7438 (7C23)_PCR/3104 [Gene]SCO7438/2826 NC_003888 SC6D11.34, possible LysR-family transcriptional regulatory protein, len: 311 aa. Similar to many LysR-family regulators involved in the control of nodulation e.g. Rhizobium sp. (strain NGR234) SW:NOD1_RHISN(EMBL:AE000065) nodulation protein D I (322 aa), fasta scores opt: 449 z-score: 537.5 E(): 1.6e-22 32.3% identity in 294 aa overlap. Contains a Prosite hit to PS00044 Bacterial regulatory proteins, lysR family signature and Pfam matches to PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and Pfam match to entry PF01046 NodD_C_term, NodD transcription activator carboxyl terminal region. The putative helix-turn-helix motif situated between residues 19..40 (+4.28 SD). 3 2 4 14 [Reporter]SCO7174 (8O19)_PCR/3103 [Gene]SCO7174/2825 NC_003888 SC8A11.02c, unknown, len: 89 aa 3 2 4 13 [Reporter]SCO6446 (9K19)_PCR/3102 [Gene]SCO6446/2824 NC_003888 SC9B5.13, unknown, len: 319aa. 3 2 4 12 [Reporter]SCO2299 (10G19)_PCR/3101 [Gene]SCO2299/2823 NC_003888 SCC30.07c, possible bifunctional protein (ribonuclease H/phosphoglycerate mutase), len: 497 aa; N-terminal region similar to TR:Q9X7R6 (EMBL:AL049863) Streptomyces coelicolor putative ribonuclease H SC5H1.08c, 235 aa; blastp scores: Score = 185 (65.1 bits), Expect = 9.5e-14, P = 9.5e-14 Identities = 82/230 (35%), Positives = 105/230 (45%) and C-terminal region similar to TR:O67797 (EMBL:AE000766) Aquifex aeolicus phosphoglycerate mutase PgmA, 212 aa; fasta scores: opt: 355 z-score: 327.3 E(): 8.7e-11; 32.8% identity in 201 aa overlap. Contains Pfam match to entry PF00075 rnaseH, RNase H and PF00300 PGAM, Phosphoglycerate mutase family 3 2 4 11 [Reporter]SCO4586 (11C19)_PCR/3100 [Gene]SCO4586/2822 NC_003888 SCD20.04, possible ABC transporter integral membrane protein, len: 309 aa; similar to TR:CAB75311 (EMBL:AL139164) Streptomyces coelicolor putative integral membrane protein SCC46.07c, 299 aa; fasta scores: opt: 412 z-score: 436.8 E(): 7.3e-17; 27.6% identity in 297 aa overlap. Contains possible hydrophobic membrane spanning regions 3 2 4 10 [Reporter]SCO3299 (12O15)_PCR/3099 [Gene]SCO3299/2821 NC_003888 SCE15.16c, unknown, len: 397aa; similar to many e.g. SW:RTCB_ECOLI hypothetical protein from Escherichia coli (408 aa) fasta scores; opt: 809, z-score: 903.2, E(): 0, 46.8% identity in 408 aa overlap. Contains Pfam match to entry PF01139 UPF0027, Uncharacterized protein family UPF0027. 3 2 4 9 [Reporter]SCO0508 (13K15)_PCR/3098 [Gene]SCO0508/2820 NC_003888 SCF6.04, possible tetR family transcriptional regulator, len: 180 aa. Similar to many e.g. Streptomyces glaucescens SW:TCMR_STRGA (EMBL:M80674) tetracenomycin C transcriptional repressor (226 aa), fasta scores opt: 225 z-score: 288.9 E(): 1e-08 34.5% identity in 174 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the possible helix-turn-helix motif between residues 13..34 (+3.92 SD). 3 2 4 8 [Reporter]SCO3487 (14G15)_PCR/3097 [Gene]SCO3487/2819 NC_003888 SCE65.23, possible hydrolase, len: 798 aa; similar to SW:AGAB_VIBS7 (EMBL:D21202) Vibrio sp. (strain JT0107) beta-agarase B (EC 3.2.1.81) AgaB, 955 aa; fasta socres: opt: 1394 z-score: 1581.4 E(): 0; 39.0% identity in 772 aa overlap. Contains possible hydrophobic membrane spanning region and Leucine TTA codon, possible target for bldA regulation 3 2 4 7 [Reporter]SCO1774 (15C15)_PCR/3096 [Gene]SCO1774/2818 NC_003888 SCI51.14c, probable regulatory protein, len: 682 aa; similar to the C-terminal region of other regulators from S.coelicolor e.g. SW:AFSR_STRCO (EMBL:D90155), AfsR, Streptomyces coelicolor global regulatory protein for secondary metabolite formation (993 aa), fasta scores; opt: 474 z-score: 505.1 E(): 8.8e-21, 27.7% identity in 656 aa overlap. The N-terminal and C-terminal regions of AfsR have been shown to direct actinorhodin production independently. The N-terminal half of this protein is also weakly similar to the C-terminal region of TR:Q9Z389 (EMBL:AL035654), CdaR, S.coelicolor transcriptional activator protein for CDA biosynthesis (592 aa) (28.6% identity in 332 aa overlap) 3 2 4 6 [Reporter]SCO2952 (16O11)_PCR/3095 [Gene]SCO2952/2817 NC_003888 SCE59.11c, possible helicase protein, len: 744 aa; similar to TR:O32215 (EMBL:Z99121) Bacillus subtilis YvgS protein, 774 aa; fasta scores: opt: 346 z-score: 378.5 E(): 1.1e-13; 23.1% identity in 808 aa overlap and to C-terminal region of SW:UVRD_MYCLE (EMBL:U00016) Mycobacterium leprae probable DNA helicase II homolog (EC 3.6.1.-) UvrD, 457 aa; fasta scores: opt: 155 z-score: 172.1 E(): 0.036; 26.6% identity in 241 aa overlap. Contains Pfam match to entry PF01443 Viral_helicase1, Viral (Superfamily 1) RNA helicase and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Contains possible helix-turn-helix motif at residues 638..659 (+2.82 SD) 3 2 4 5 [Reporter]SCO1198 (17K11)_PCR/3094 [Gene]SCO1198/2816 NC_003888 SCG11A.29c, probable acyl-CoA dehydrogenase, len:393 aa; similar to TR:Q54857 (EMBL:) Streptomyces purpurascens acyl-CoA dehydrogenase FamB (fragment), 288 aa; fasta scores: opt: 1195 z-score: 1377.5 E(): 0; 83.2% identity in 208 aa overlap and to SW:ACD_MYCLE (EMBL:U00012) Mycobacterium leprae acyl-CoA dehydrogenase (EC 1.3.99.-) Acd or B1308_F1_34, 389 aa; fasta scores: opt: 386 z-score: 447.1 E(): 1.6e-17; 28.5% identity in 376 aa overlap. Contains two matches to Pfam entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase 3 2 4 4 [Reporter]SCO3420 (18G11)_PCR/3093 [Gene]SCO3420/2815 NC_003888 SCE9.27c, probable aldehyde dehydrogenase, len: 486 aa; highly similar to many NAD-linked aldehyde dehydrogenases e.g. SW:XYLC_PSEPU (EMBL:U15151), xylC, Pseudomonas putida benzaldehyde dehydrogenase [NAD+] from TOL plasmid pWW0 (487 aa), fasta scores; opt: 1244 z-score: 1420.3 E(): 0, 41.5% identity in 479 aa overlap. Similar to others from S.coelicolor e.g. TR:O88069 (EMBL:AL031541) probable aldehyde dehydrogenase (483 aa) (40.0% identity in 448 aa overlap). Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase, score 491.30, E-value 7.6e-144, PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site 3 2 4 3 [Reporter]SCO4050 (19C11)_PCR/3092 [Gene]SCO4050/2814 NC_003888 2SCD60.16, possible acetyltransferase, len: 206 aa; similar to TR:P94254 (EMBL:U61153) Bordetella bronchiseptica alcaligin biosynthesis protein AlcB, 201 aa; fasta scores: opt: 325 z-score: 399.6 E(): 1.1e-14; 37.2% identity in 183 aa overlap and to SW:AAC6_SERMA (EMBL:M23634) Serratia marcescens aminoglycoside N6'-acetyltransferase (EC 2.3.1.82) AacA4, 201 aa; fasta scores: opt: 155 z-score: 196.0 E(): 0.0023; 25.7% identity in 171 aa overlap 3 2 3 22 [Reporter]SCO4189 (1K3)_PCR/3089 [Gene]SCO4189/2813 NC_003888 2SCD46.03c, unknown, len: 62aa; strongly similar to neighbouring CDS 2SCD46.03c fasta scores; opt: 169, z-score: 236.7, E(): 1.4e-07, 51.1% identity in 45 aa overlap. Hydrophobic. 3 2 3 21 [Reporter]SCO0742 (2G3)_PCR/3088 [Gene]SCO0742/2812 NC_003888 SCF81.01, ABC transporter (partial CDS), len: >38 aa; identical to C-terminal region of previously sequenced TR:Q9ZNB0 (EMBL:AB019513) Streptomyces coelicolor ABC transporter, 584 aa,3SC5B7.20, ABC transporter ATP-binding protein (fragment), len: >578 aa; identical to previously sequenced TR:Q9ZNB0 (EMBL:AB019513) Streptomyces coelicolor ABC transporter, 584 aa. Contains Pfam match to entry PF00664 ABC_membrane, ABC transporter transmembrane region and PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also contains possible hydrophobic membrane spanning regions 3 2 3 20 [Reporter]SCO2608 (3C3)_PCR/3087 [Gene]SCO2608/2811 NC_003888 SCC88.19c, pbp2, penicillin binding protein, len: 769 aa; almost identical to previously sequenced TR:O33622 (EMBL:Y14206) Streptomyces coelicolor Pbp protein and similar to SW:PBP2_ECOLI (EMBL:X04516) Escherichia coli penicillin-binding protein 2 (Pbp-2), 633 aa; fasta scores: opt: 226 z-score: 236.5 E(): 9.3e-06; 27.9% identity in 692 aa overlap. Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain 3 2 3 19 [Reporter]SCO6144 (3O23)_PCR/3086 [Gene]SCO6144/2810 NC_003888 SC1A9.08c, possible transcriptional regulator, len: 189 aa; similar to TR:O34892 (EMBL:AF027868) proposed transcriptional regulator from Bacillus subtilis (191 aa) fasta scores; opt: 302, z-score: 488.2, E(): 6.6e-20, (31.8% identity in 173 aa overlap). Contains an helix-turn-helix motif from: 1 to: 189, Score 1074 (+2.84 SD). 3 2 3 18 [Reporter]SCO2031 (4K23)_PCR/3085 [Gene]SCO2031/2809 NC_003888 SC3A3.09, hypothetical protein, len: 467 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Shows weak similarity to the neighbouring CDS SC3A3.06, SC3A3.07 and SC3A3.08 e.g. SC3A3.06 possible membrane protein (509 aa) (33.1% identity in 353 aa overlap). Has a Pro-rich C-terminus 3 2 3 17 [Reporter]SCO6554 (5G23)_PCR/3084 [Gene]SCO6554/2808 NC_003888 SC4B5.04, probable transcriptional regulatory protein, len: 327 aa; C-terminal region weakly similar to several members of the asnC family of transcriptional regulators e.g. SW:Y723_METJA putative transcriptional regulatory protein from Methanococcus jannaschii (156 aa), fasta scores; opt: 167, z-score: 267.8, E(): 1.3e-07, (24.1% identity in 141 aa overlap). Contains probable helix-turn-helix motif (score 1226, +3.36 SD) from residue 198 to 219. 3 2 3 15 [Reporter]SCO4416 (7O19)_PCR/3082 [Gene]SCO4416/2807 NC_003888 SC6F11.14c, probable monooxygenase, len: 401 aa; similar to TR:Q9SDE6 (EMBL:AP000837) Oryza sativa similar to human dimethylanilyne monooxygenase, 437 aa; fasta scores: opt: 692 z-score: 722.8 E(): 9.3e-33; 38.2% identity in 403 aa overlap. Contains Pfam match to entry PF00743 FMO-like, Flavin-binding monooxygenase-like 3 2 3 14 [Reporter]SCO7599 (8K19)_PCR/3081 [Gene]SCO7599/2806 NC_003888 SC7H9.11, probable transcriptional regulator, len: 311 aa; similar to SW:HEXR_ECOLI (EMBL:AE000279) Escherichia coli Hex regulon repressor HexR, 289 aa; fasta scores: opt: 428 z-score: 486.6 E(): 1.4e-19; 31.9% identity in 285 aa overlap. Contains Pfam match to entry PF01418 HTH_6, Helix-turn-helix domain, rpiR family and PF01380 SIS, SIS domain. Also contains possible helix-turn-helix motif at residues 54..75 (+3.19 SD) 3 2 3 13 [Reporter]SCO7157 (9G19)_PCR/3080 [Gene]SCO7157/2805 NC_003888 SC9A4.19c, possible integral membrane protein, len: aa; similar to TR:Q9S277 (EMBL:AL096844) Streptomyces coelicolor putative integral membrane protein SCI28.06c, 421 aa; fasta scores: opt: 1032 z-score: 1103.0 E(): 0; 77.2% identity in 189 aa overlap. Contains possible hydrophobic membrane spanning regions 3 2 3 12 [Reporter]SCO2294 (10C19)_PCR/3079 [Gene]SCO2294/2804 NC_003888 SCC30.02c, probable araC-family regulatory protein, len: 278 aa; similar to SW:ARAC_SALTY (EMBL:J01797) Salmonella typhimurium arabinose operon regulatory protein AraC, 281 aa; fasta scores: opt: 232 z-score: 270.4 E(): 1.3e-07; 26.7% identity in 270 aa overlap and to various Streptomyces coelicolor putative araC-family transcriptional regulators, e.g. TR:O50480 (EMBL:AL031031) S. coelicolor putative transcriptional regulator SC7C7.17 (SC4H8.01), 288 aa; fasta scores: opt: 638 z-score: 605.1 E(): 3.3e-28; 43.8% identity in 292 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family and possible helix-turn-helix motif at residues 189..210 (+3.81 SD) 3 2 3 11 [Reporter]SCO4511 (11O15)_PCR/3078 [Gene]SCO4511/2803 NC_003888 SCD35.18c, possible integral membrane protein, len: 139 aa. Contains possible hydrophobic membrane spanning region 3 2 3 10 [Reporter]SCO3295 (12K15)_PCR/3077 [Gene]SCO3295/2802 NC_003888 SCE15.12c, possible oxidoreductase, len: 222aa; similar to TR:O85762 (EMBL:AF075709) NADH dependent FMN reductase from Pseudomonas putida (200 aa) fasta scores; opt: 208, z-score: 241.1, E(): 4e-06, (35.7% identity in 129 aa overlap). 3 2 3 9 [Reporter]SCO3230 (13G15)_PCR/3076 [Gene]SCO3230/2801 NC_003888 SCE63.03c, cdaPSI, CDA peptide synthetase I, len: 7463 aa; part of the calcium-dependent antibiotic (CDA) biosynthetic cluster from Streptomyces coelicolor. CDA is a peptide antibiotic which is synthesised non-ribosomally by a putative multifunctional peptide synthetase enzyme. This CDS encodes a subunit of this enzyme and is suspected to be responsible for the incorperation of the first 6 amino acids into the antibiotic structure. This ORF overlaps the downstream (cdaPSII) ORF by one base indicating possible translational coupling. Contains eight separate Pfam matches to entry PF00668 DUF4, Domain of unknown function (U), six separate Pfam matches to entry PF00501 AMP-binding, AMP-binding enzyme (A) and six separate Pfam matches to entry PF00550 pp-binding, Phosphopantetheine attachment site (P). These Pfam matches cover the full length of the protein in the following order from N to C-terminal U-A-P-U-A-P-U-A-P-U-U-A-P-U-A-P-U-A-P-U. 3 2 3 8 [Reporter]SCO0630 (14C15)_PCR/3075 [Gene]SCO0630/2800 NC_003888 SCF56.14c, possible acetyltransferase, len: 173 aa; similar to SW:YJHQ_ECOLI (EMBL:U14003) Escherichia coli hypothetical 20.0 kD protein YjhQ, 181 aa; fasta scores: opt: 248 z-score: 301.7 E(): 2e-09; 36.2% identity in 163 aa overlap and weakly similar to SW:PUAC_STRLP (EMBL:M25346) Streptomyces lipmanii (Streptomyces alboniger) puromycin N-acetyltransferase (EC 2.3.-.-) PuaC, 199 aa; fasta scores: opt: 123 z-score: 155.6 E(): 0.28; 31.8% identity in 107 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 4 3 23 16 [Reporter]SCO2671 (7D18)_PCR/6041 [Gene]SCO2671/5499 NC_003888 SC6D10.14, possible globin, len: 137 aa; similar to TR:O53197 (EMBL:AL021246) Mycobacterium tuberculosis putative globin, 128 aa; fasta scores: opt: 466 z-score: 620.8 E(): 3.6e-27; 53.6% identity in 125 aa overlap and to SW:GLBN_NOSSN (EMBL:L47979) Nostoc sp. cyanoglobin GlbN, 118 aa; fasta scores: opt: 170 z-score: 236.1 E(): 9.7e-06; 25.2% identity in 119 aa overlap. Contains Pfam match to entry PF01152 Bac_globin, Protozoan/cyanobacterial globin 4 3 23 15 [Reporter]SCO1997 (8P14)_PCR/6040 [Gene]SCO1997/5498 NC_003888 SC7H2.11c, unknown, len: 312 aa; similar to TR:O07213 (EMBL:Z96072) hypothetical protein from Mycobacterium tuberculosis (324 aa) fasta scores; opt: 770, z-score: 866.1, E(): 0, (40.9% identity in 286 aa overlap) 4 3 23 14 [Reporter]SCO5851 (9L14)_PCR/6039 [Gene]SCO5851/5497 NC_003888 SC9B10.18, possible integral membrane protein, len: 312 aa. Contains possible hydrophobic membrane spanning regions 4 3 23 13 [Reporter]SCO1938 (10H14)_PCR/6038 [Gene]SCO1938/5496 NC_003888 SCC22.20, hypothetical protein, len: 351 aa; similar to TR:O88016 (EMBL:AL031107) hypothetical protein from Streptomyces coelicolor (311 aa) fasta scores; opt: 1363, z-score: 1605.9, E(): 0, (71.3% identity in 300 aa overlap) and TR:O06813 (EMBL:Z95844) hypothetical protein from Mycobacterium tuberculosis (303 aa) fasta scores; opt: 849, z-score: 1002.6, E(): 0, (46.3% identity in 309 aa overlap). 4 3 23 12 [Reporter]SCO4101 (11D14)_PCR/6037 [Gene]SCO4101/5495 NC_003888 SCD17.05, possible secreted protein, len: 130 aa. Contains a possible N-terminal signal sequence. 4 3 23 11 [Reporter]SCO4154 (12P10)_PCR/6036 [Gene]SCO4154/5494 NC_003888 SCD84.21, hypothetical protein, len: 142 aa; similar to TR:Q9ZBJ6 (EMBL:AL035161) Streptomyces coelicolor conserved hypothetical protein SC9C7.14c, 132 aa; fasta scores: opt: 186 z-score: 244.7 E(): 3.6e-06; 34.9% identity in 106 aa overlap. Contains Pfam match to entry PF01042 UPF0076, Domain of unknown function 4 3 23 10 [Reporter]SCO3117 (13L10)_PCR/6035 [Gene]SCO3117/5493 NC_003888 SCE41.26, unknown, len: 176 aa 4 3 23 9 [Reporter]SCO1633 (14H10)_PCR/6034 [Gene]SCO1633/5492 NC_003888 SCI41.16c, probable secreted protein, len: 95 aa. Similar to various hypothetical proteins, e.g. SW:YY34_MYCLE (EMBL:U00017) Mycobacterium leprae hypothetical 9.8 kd protein U2126B, 88 aa; fasta scores: opt: 221 z-score: 258.0 E(): 5.3e-07; 45.3% identity in 86 aa overlap 4 3 23 7 [Reporter]SCO1201 (16P6)_PCR/6032 [Gene]SCO1201/5491 NC_003888 2SCG58.01, possible reductase (fragment), len: >43 aa; C-terminal part of TR:Q9RJW9 (EMBL:AL133210) Streptomyces coelicolor putative reductase (fragment) SCF37.32, 311 aa,SCG11A.32, possible reductase, len: >310 aa; similar to TR:Q9Z9W3 (EMBL:AB011836) Bacillus sp. (strain C-125) alkyl hydroperoxide reductase large subunit (EC 1.6.99.3) AhpF, 305 aa; fasta scores: opt: 668 z-score: 747.2 E(): 0; 37.3% identity in 295 aa overlap and to SW:AHPF_PSEPU (EMBL:AB010689) Pseudomonas putida alkyl hydroperoxide reductase subunit F (EC 1.6.4.-) AhpF, 520 aa; fasta scores: opt: 338 z-score: 378.9 E(): 9.9e-14; 28.6% identity in 308 aa overlap 4 3 23 6 [Reporter]SCO0801 (17L6)_PCR/6031 [Gene]SCO0801/5490 NC_003888 SCF43.12, possible cytochrome P450, len: 527 aa. Highly similar to the N-terminal P450 domain of Bacillus megaterium SW:CPXB_BACME (EMBL:J04832) cytochrome P450(BM-3) (1048 aa), fasta scores opt: 789 z-score: 839.8 E():0 41.9% identity in 497 aa overlap. Also weakly similar to Eukaryotic cytochrome P450 proteins e.g. Homo sapiens (Human) SW:CPF3_HUMAN(EMBL:D12620) cytochrome P450 4F3 (leukotriene-B4 20-monooxygenase) (EC 1.14.13.30) (520 aa), fasta scores opt: 553 z-score: 592.7 E(): 1.2e-25 29.2% identity in 390 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450 and a Prosite hit to PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 4 3 23 5 [Reporter]SCO0829 (18H6)_PCR/6030 [Gene]SCO0829/5489 NC_003888 SCF43A.19, probable secreted serine protease, len: 458 aa; similar to many serine proteases e.g. SW:PRTC_STRGR (EMBL:L29018), SprC, Streptomyces griseus serine protease C precursor (457 aa), fasta scores; opt: 1563 z-score: 1510.8 E(): 0, 54.1% identity in 449 aa overlap. Both have a C-terminal region similar to part of some chitinases e.g. SW:CHI1_BACCI (EMBL:M57601), chiA1, Bacillus circulans chitinase A1 precursor, which is not found in the other similar serine proteases. The Thr/Pro-rich 19-amino acid linker region previously identified in SW:PRTC_STRGR is shortened in the S.coelicolor homologue. Contains probable N-terminal signal sequence. Weakly similar to TR:O54109 (EMBL:AL021529) S.coelicolor probable secreted protease (411 aa) (29.4% identity in 418 aa overlap). Contains Pfam match to entry PF00089 trypsin, Trypsin and PS00135 Serine proteases, trypsin family, serine active site 4 3 23 4 [Reporter]SCP1.239c (19D6)_PCR/6029 [Gene]SCP1.239c/5488 NC_003888 SCP1.239c, mmyG, possible oxidoreductase, len: 393aa; previously sequenced and annotated as TR:Q9JN86 (EMBL:AJ276673). Similar to many eg. TR:Q9RK99 (EMBL:AL117322) putative oxidoreductase from Streptomyces coelicolor (371 aa) fasta scores; opt: 675, z-score: 754.9, E(): 0, 36.4% identity in 357 aa overlap. Contains Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family. 4 3 23 3 [Reporter]SCO4889 (20P2)_PCR/6028 [Gene]SCO4889/5487 NC_003888 2SCK8.15, probable cytidine deaminase, len: 130 aa; similar to SW:CDD_HUMAN (EMBL:L27943) Homo sapiens cytidine deaminase (EC 3.5.4.5) Cda or Cdd, 146 aa; fasta scores: opt: 297 z-score: 380.0 E(): 1.3e-13; 36.2% identity in 130 aa overlap and to TR:53367 (EMBL:AL021841) Mycobacterium tuberculosis cytidine deaminase Cdd or MTV016.15c, 133 aa; fasta scores: opt: 518 z-score: 652.9 E(): 8.4e-29; 61.1% identity in 126 aa overlap. Contains Pfam match to entry PF00383 dCMP_cyt_deam, Cytidine and deoxycytidylate deaminase zinc-binding region and match to Prosite entry PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature 4 3 22 22 [Reporter]SCO1087 (1H22)_PCR/6026 [Gene]SCO1087/5486 NC_003888 2SCG4.03c, probable aldolase, len: 356 aa; similar to TR:O50584 (EMBL:AB001577) Pseudomonas sp. low specificity L-threonine aldolase, 346 aa; fasta scores: opt: 832 z-score: 977.3 E(): 0; 41.3% identity in 339 aa overlap and to SW:LTAA_AERJA (EMBL:D87890) Aeromonas jandaei L-allo-threonine aldolase (EC 4.1.2.-) LtaA, 338 aa; fasta scores: opt: 270 z-score: 321.1 E(): 2.1e-10; 26.9% identity in 324 aa overlap 4 3 22 21 [Reporter]SCO4469 (2D22)_PCR/6025 [Gene]SCO4469/5485 NC_003888 SCD65.12, hemL, glutamate-1-semialdehyde 2,1-aminomutase, len: 438 aa; highly similar to SW:GSA_ECOLI (EMBL:X53696) Escherichia coli glutamate-1-semialdehyde 2,1-aminomutase (EC 5.4.3.8) HemL, 426 aa; fasta scores: opt: 1425 z-score: 1497.5 E(): 0; 52.0% identity in 425 aa overlap. Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate and match to Prosite entry PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site 4 3 22 20 [Reporter]SCO6426 (3P18)_PCR/6024 [Gene]SCO6426/5484 NC_003888 SC1A6.15, unknown, len: 170 aa; similar to the C-terminus of several ABC transporter ATP binding proteins involved in iron transport, e.g. FEPC_ECOLI P23878 Escherichia coli ferric enterobactin transport ATP binding protein (271 aa), fasta scores; opt: 237 z-score: 425.9 E(): 1.3e-16, 38.7% identity in 106 aa overlap 4 3 22 19 [Reporter]SCO7610 (4L18)_PCR/6023 [Gene]SCO7610/5483 NC_003888 SC2H2.08, possible transcriptional regulator, len: 371 aa; similar to many other Streptomyces coelicolor putative transcriptional regulators, e.g. TR:Q9KYU5 (EMBL:AL355832) putative DeoR-family transcriptional regulator SCE22.17, 323 aa; fasta scores: opt: 458 z-score: 518.3 E(): 2.4e-21; 32.3% identity in 319 aa overlap. Contains possible helix-turn-helix motif at residues 24..45 (+3.61 SD) 4 3 22 18 [Reporter]SCO6829 (5H18)_PCR/6022 [Gene]SCO6829/5482 NC_003888 SC4A9.06c, possible oxidoreductase, len: 259 aa. Highly similar to Streptomyces coelicolor TR:Q9X890(EMBL:AL049707) putative oxidoreductase, SCE15.15 (260 aa), fasta scores opt: 1088 z-score: 1221.9 E(): 0 66.9% identity in 254 aa overlap. Also similar to Streptomyces griseus subsp. griseus TR:Q9XDF5(EMBL:AF074603) putative ketoacyl reductase (271 aa), fasta scores opt: 421 z-score: 479.1 E(): 2.8e-19 36.7% identity in 256 aa overlap. Contains a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature and a Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 4 3 22 17 [Reporter]SCO7554 (6D18)_PCR/6021 [Gene]SCO7554/5481 NC_003888 SC5F1.08c, probable lacI-family transcriptional regulator, len: 337 aa; similar to TR:Q9X9R3 (EMBL:AJ009798) Streptomyces reticuli CebR protein, 350 aa; fasta scores: opt: 1152 z-score: 1278.6 E(): 0; 54.6% identity in 339 aa overlap and to SW:CELR_THEFU (EMBL:AF086819) Thermomonospora fusca transcription regulator CelR, 340 aa; fasta scores: opt: 1031 z-score: 1145.4 E(): 0; 52.1% identity in 340 aa overlap. Also similar to TR:CAC10103 (EMBL:AL442165) Streptomyces coelicolor lacI-family transcriptional regulatory protein CebR 2SCC13.02c, 351 aa; fasta scores: opt: 1082 z-score: 996.3 E(): 0; 52.8% identity in 339 aa overlap. Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family. Also contains possible helix-turn-helix motif at residues 7..28 (+4.69 SD) 4 3 22 16 [Reporter]SCO1411 (7P14)_PCR/6020 [Gene]SCO1411/5480 NC_003888 SC6D7.28c, possible transmembrane transport protein, len: 440 aa. Highly similar to many other membrane transport proteins e.g. Escherichia coli SW:CIT1_ECOLI(EMBL:M11559) citrate-proton symporter (citrate transporter) (431 aa), fasta scores opt: 349 z-score: 368.9 E(): 3.6e-13 25.1% identity in 438 aa overlap and Yersinia pestis TR:Q9ZC42(EMBL:AL031866) putative transport protein (409 aa), fasta scores opt: 1363 z-score: 1421.9 E():0 49.5% identity in 410 aa overlap. Contains a Prosite hit to PS00217 Sugar transport proteins signature 2 and a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter. Also contains multiple possible membrane spanning hydrophobic domains. 4 3 22 15 [Reporter]SCO1993 (8L14)_PCR/6019 [Gene]SCO1993/5479 NC_003888 SC7H2.07c, unknown, len: 228aa; 4 3 22 14 [Reporter]SCO7530 (9H14)_PCR/6018 [Gene]SCO7530/5478 NC_003888 SC8G12.06c, possible regulatory protein, len: 360 aa. The N-terminus of this protein is similar to many regulatory proteins e.g. Deinococcus radiodurans TR:AAF11989(EMBL:AE002074) transcriptional regulator, MerR family (280 aa), fasta scores opt: 324 z-score: 367.6 E(): 5.1e-13 37.3% identity in 244 aa overlap. The C-terminus of this protein is similar to many hypothetical proteins and putative phosphatases e.g. Mycobacterium leprae TR:Q50188(EMBL:Z70722) putative phosphoprotein phosphatase (509 aa), fasta scores opt: 709 z-score: 790.9 E(): 0 54.6% identity in 238 aa overlap. Contains a Prosite hit to PS00552 Bacterial regulatory proteins, merR family signature and a Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family. 4 3 22 13 [Reporter]SCO1933 (10D14)_PCR/6017 [Gene]SCO1933/5477 NC_003888 SCC22.15c, unknown, len: 144aa; 4 3 22 12 [Reporter]SCO4335 (11P10)_PCR/6016 [Gene]SCO4335/5476 NC_003888 SCD12A.18c, unknown, len: 62 aa 4 3 22 11 [Reporter]SCO4150 (12L10)_PCR/6015 [Gene]SCO4150/5475 NC_003888 SCD84.17, possible ABC transport system transmembrane protein, len: 206 aa; similar to TR:O69958 (EMBL:AL022268) Streptomyces coelicolor putative transmembrane transport protein SC4H2.03c, 411 aa; fasta scores: opt: 136 z-score: 161.5 E(): 0.16; 30.8% identity in 185 aa overlap. Contains possible hydrophobic membrane sapnning regions 4 3 22 10 [Reporter]SCO3000 (13H10)_PCR/6014 [Gene]SCO3000/5474 NC_003888 SCE33.02c, possible phosphatase, len: 223 aa; similar to TR:AAF42165 (EMBL:AE002532) Neisseria meningitidis phosphoglycolate phosphatase, putative NMB1830, 219 aa; fasta scores: opt: 221 z-score: 270.1 E(): 1.4e-07; 28.4% identity in 218 aa overlap and to SW:GPH_RHOCA (EMBL:U23145) Rhodobacter capsulatus phosphoglycolate phosphatase (EC 3.1.3.18) CbbZ, 219 aa; fasta scores: opt: 158 z-score: 196.2 E(): 0.0018; 25.4% identity in 189 aa overlap. Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase 4 3 22 9 [Reporter]SCO0608 (14D10)_PCR/6013 [Gene]SCO0608/5473 NC_003888 StF55.32, possible regulatory protein, len: 292 aa; similar to SW:ALGP_PSEAE (EMBL:M30145) Pseudomonas aeruginosa transcriptional regulatory protein (alginate regulatory protein) AlgP, 340 aa; fasta scores: opt: 239 z-score: 217.9 E(): 9.1e-05; 35.8% identity in 193 aa overlap. Contains Lysine/Alanine rich region 4 3 22 8 [Reporter]SCO2966 (15P6)_PCR/6012 [Gene]SCO2966/5472 NC_003888 SCE59.25c, smpB, small protein B homologue, len: 159 aa; highly similar to SW:SMPB_ENTFA (EMBL:M90060) Enterococcus faecalis small protein B homologue SmpB, 154 aa; fasta scores: opt: 559 z-score: 693.2 E(): 3.3e-31; 56.1% identity in 155 aa overlap. Contains Pfam match to entry PF01668 SmpB, SmpB protein and match to Prosite entry PS01317 Protein smpB signature 4 2 5 14 [Reporter]SCO5942 (8D23)_PCR/3629 [Gene]SCO5942/3299 NC_003888 SC7H1.12, unknown, len: 205 aa; very weak similarity to downstream ORF SC7H1.21 (203 aa), (E(): 0.085, 26.9% identity in 208 aa overlap). Contains possible Helix-turn-helix motif at aa 41-62, Score 1089 (+2.90 SD) 4 3 22 7 [Reporter]SCO0461 (16L6)_PCR/6011 [Gene]SCO0461/5471 NC_003888 SCF76.01, possible hydrolase, partial CDS, len: >228 aa. Similar to several hypothetical proteins e.g. from Saccharomyces cerevisiae SW:YN93_YEAST (EMBLL:Z71679) (290 aa), fasta scores opt: 609 z-score: 749.0 E():0 42.4% identity in 229 aa overlap. Also weakly similar to Xanthobacter autotrophicus SW:HALO_XANAU (EMBL:M26950) haloalkane dehalogenase (EC 3.8.1.5) (310 aa), fasta scores opt: 151 z-score: 190.4 E():0.003 22.9% identity in 231 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold,SCF51A.39, possible hydrolase, partial CDS, len: >113 aa. Highly similar to Saccharomyces cerevisiae (Bakers yeast) SW:YN93_YEAST (EMBL; Z71679) hypothetical 32.8 KD protein in BIO3-HXT17 intergenic region. In addition to being similar to many dehalogenases e.g. Mycobacterium tuberculosis SW:YU14_MYCTU (EMBL; Z77163) putative haloalkane dehalogenase (EC 3.8.1.5) (300 aa), fasta scores opt: 222 z-score: 292.5 E(): 6.2e-09 37.0% identity in 100 aa overlap 4 2 5 13 [Reporter]SCO6451 (9P19)_PCR/3628 [Gene]SCO6451/3298 NC_003888 SC9B5.18, probable substrate binding protein, len: 467aa; First of a five gene operon probably involved in peptide uptake. Very similar to SW:XP55_STRLI protein precursor of XP55, a major secreted protein from Streptomyces lividans (542 aa) fasta scores: opt: 3353, z-score: 2613.8, E(): 0, (95.9% identity in 532 aa overlap). Also similar to many substrate binding /transport proteins eg. SW:APPA_BACSU AppA oligopeptide-binding protein precursor from Bacillus subtilis (543 aa) fasta scores: opt: 717, z-score: 518.2, E(): 1.4e-21, (28.7% identity in 550 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site and PS01040 Bacterial extracellular solute-binding proteins, family 5 signature. Also contains Pfam match to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5. 4 3 22 6 [Reporter]SCO3567 (17H6)_PCR/6010 [Gene]SCO3567/5470 NC_003888 SCH17.01c, probable serine protease, partial CDS, len: >54 aa; continues as SCH5.30c in cosmid H5 (EMBL:AL035636). Alternative start codons are present at codons 3 and 8,SCH5.30c, partial CDS, probable serine protease, len: >385aa; similar to many eg. TR:O69639 (EMBL:AL022121) proposed serine protease from Mycobacterium tuberculosis (397 aa) fasta scores; opt: 835, z-score: 938.4, E(): 0, (39.9% identity in 386 aa overlap) and TR:Q44597 (EMBL:U07352) htrA, serine protease from Brucella abortus (513 aa) fasta scores; opt: 222, z-score: 251.4, E(): 1.1e-06, (30.6% identity in 180 aa overlap). 4 2 5 12 [Reporter]SCO2733 (10L19)_PCR/3627 [Gene]SCO2733/3297 NC_003888 SCC57A.04c, unknown, len: 279 aa. Similar to several proteins of unconfirmed function including: Streptomyces coelicolor TR:CAB58282(EMBL:AL121850) conserved hypothetical protein SCC78.01C (fragment), fasta scores opt: 274 z-score: 319.9 E(): 2e-10 35.3% identity in 235 aa overlap and Streptomyces nogalater TR:AAF01810(EMBL:AF187532) putative hydroxylase (275 aa), fasta scores opt: 384 z-score: 443.4 E(): 2.6e-17 34.9% identity in 281 aa overlap. 4 2 5 11 [Reporter]SCO4591 (11H19)_PCR/3626 [Gene]SCO4591/3296 NC_003888 SCD20.09, hypothetical protein, len: 190 aa; similar to TR:Q9RJT3 (EMBL:AL132707) Streptomyces coelicolor hypothetical 21.7 kDa protein SCF51.02c, 205 aa; fasta scores: opt: 325 z-score: 395.2 E(): 1.5e-14; 40.4% identity in 166 aa overlap 4 2 5 10 [Reporter]SCO4297 (12D19)_PCR/3625 [Gene]SCO4297/3295 NC_003888 SCD95A.30, probable oxidoreduxtase, len: 376 aa; similar to SW:NADO_THEBR (EMBL:X67220) Thermoanaerobacter brockii NADH oxidase, 651 aa; fasta scores: opt: 498 z-score: 552.0 E(): 2.8e-23; 33.6% identity in 354 aa overlap. Contains Pfam match to entry PF00724 oxidored_FMN, FMN oxidoreductase 4 2 5 9 [Reporter]SCO0233 (13P15)_PCR/3624 [Gene]SCO0233/3294 NC_003888 SCJ9A.12c, possible DNA-binding protein, len: 279 aa; N-terminal similar to TR:O86721 (EMBL:AL031515) hypothetical protein from Streptomyces coelicolor (272 aa) fasta scores; opt: 135, z-score: 165.1, E(): 0.079, (29.3% identity in 150 aa overlap). Contains possible helix-turn-helix motif (+3.31 SD) 37-58 aa 4 2 5 8 [Reporter]SCO0279 (14L15)_PCR/3623 [Gene]SCO0279/3293 NC_003888 SCF85.07, possible glycosyl hydrolase, len; 423 aa. Shares a low level of similarity with many alpha-l-fucosidases e.g. Thermotoga maritima TR:AAD35394 (EMBL:AE001712) alpha-l-fucosidase, putative (449 aa), fasta scores opt: 332 z-score: 363.4 E(): 6.9e-13 35.8% identity in 358 aa overlap. 4 2 5 7 [Reporter]SCO0213 (15H15)_PCR/3622 [Gene]SCO0213/3292 NC_003888 SCJ12.25c, possible nitrate/nitrite transporter protein, len: 412 aa. Similar to Bacillus subtilis SW:NARK_BACSU (EMBL; Z49884) nitrite extrusion protein (395 aa), fasta scores opt: 834 z-score: 877.9 E():0 36.8% identity in 378 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 2 5 6 [Reporter]SCO3367 (16D15)_PCR/3621 [Gene]SCO3367/3291 NC_003888 SCE94.18, possible tetR-family regulator, len: 195 aa; similar to many regulators e.g. TR:G4240425 (EMBL:AF080235) LanK from Streptomyces cyanogenus S136 (192 aa) fasta scores; opt: 584, z-score: 737.3, E(): 0, (51.4% identity in 185 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and Prosite match to PS01081 Bacterial regulatory proteins, tetR family signature. Also contains a probable helix-turn-helix at 43-55aa (+5.78 SD). 4 2 5 5 [Reporter]SCO1061 (17P11)_PCR/3620 [Gene]SCO1061/3290 NC_003888 SCG22.07, probable bifunctional protein (secreted sugar binding protein/sugar hydrolase), len: 555 aa; N-terminal domain similar to N-terminal domain of TR:O86727 (EMBL:AL031515) Streptomyces coelicolor putative secreted cellulase SC5C7.30c, 890 aa; blastp scores: Score = 84 (29.6 bits), Expect = 0.031, Sum P(3) = 0.030 Identities = 61/233 (26%), Positives = 89/233 (38%) and C-terminal domain similar to C-terminal domain of SW:E13B_OERXA (EMBL:AF052745) Oerskovia xanthineolytica glucan enco-1,3-glucosidase precursor (EC 3.2.1.39), 548 aa; fasta scores: opt: 368 Z-score: 394.8 E(): 2.3e-14; 41.447% identity) in 152 aa overlap. Contains 3x Pfam match to entry PF02012 BNR, BNR repeat and Pfam match to entry PF00652 Ricin_B_lectin, Similarity to lectin domain of ricin beta-chain, 3 copies. Contains also possible N-terminal region signal peptide sequence 4 3 22 5 [Reporter]SCO3893 (18D6)_PCR/6009 [Gene]SCO3893/5469 NC_003888 SCH24.15c, hypothetical protein, len: 563 aa; unknown function, previously partially sequenced as TR:O86855 (EMBL:AJ007313) Streptomyces coelicolor hypothetical protein (281 aa). Weakly similar to TR:Q53839 (EMBL:U19250) Streptomyces coelicolor protein kinase-like protein (565 aa), fasta scores; opt: 205 z-score: 204.5 E(): 0.00045, 25.1% identity in 263 aa overlap and to C-terminal half of TR:O05435 (EMBL:Z94121) Mycobacterium tuberculosis hypothetical protein (1184 aa) (26.9% identity in 557 aa overlap) 4 3 22 4 [Reporter]SCP1.281 (19P2)_PCR/6008 [Gene]SCP1.281/5468 NC_003888 SCP1.281, unknown, len: 115aa; 4 3 22 3 [Reporter]SCP1.143 (20L2)_PCR/6007 [Gene]SCP1.143/5467 NC_003888 SCP1.143, possible membrane protein, len: 369aa; contains one possible membrane-spanning hydrophobic region. 4 3 21 22 [Reporter]SCO1146 (1D22)_PCR/6004 [Gene]SCO1146/5466 NC_003888 2SCG38.39c, possible lipoprotein, len: 252 aa; similar to TR:Q9RK82 (EMBL:AL132662) Streptomyces coelicolor hypothetical 26.4 kDa protein SCF11.08, 250 aa; fasta scores: opt: 645 z-score: 660.7 E(): 2.4e-29; 42.1% identity in 247 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 3 21 21 [Reporter]SCO1821 (2P18)_PCR/6003 [Gene]SCO1821/5465 NC_003888 SCI8.06c, moaA, molybdenum cofactor biosynthesis protein A, len: 341 aa. Highly similar to many molybdenum cofactor biosynthesis protein A's e.g. Mycobacterium tuberculosis TR:O53881 (EMBL:AL022004) MoaA-2 (360 aa), fasta scores opt: 1336 z-score: 1559.3 E(): 0 61.7% identity in 332 aa overlap and Staphylococcus carnosus TR:Q9ZIM6 (EMBL: AF022796) MoaA (340 aa), fasta scores opt: 627 z-score: 735.1 E():0 33.3% identity in 321 aa overlap. Contains Pfam match to entry PF01444 MoaA_NifB_PqqE, moaA / nifB / pqqE family and a Prosite hit to PS01305 moaA / nifB / pqqE family signature. 4 3 21 20 [Reporter]SCO6422 (3L18)_PCR/6002 [Gene]SCO6422/5464 NC_003888 SC1A6.11, probable two-component system regulatory protein, len: 224 aa; similar to members of the luxR/uhpA family e.g. DEGU_BACSU P13800 transcriptional regulatory protein DegU (229 aa), fasta scores; opt: 330 z-score: 706.1 E(): 3.3e-32, 36.7% identity in 226 aa overlap. Contains Pfam matches to entry response_reg PF00072, Response regulator receiver domain, score 149.95, and to entry GerE PF00196, Bacterial regulatory proteins, luxR family, score 89.64 4 3 21 19 [Reporter]SCO5610 (4H18)_PCR/6001 [Gene]SCO5610/5463 NC_003888 SC2E1.27c, unknown prophage gene, len: 120 aa; contains hydrophobic region near centre. Contains possible hydrophobic membrane spanning region 4 3 21 18 [Reporter]SCO6825 (5D18)_PCR/6000 [Gene]SCO6825/5462 NC_003888 SC4A9.02c, possible integral membrane protein, len: 231 aa. Highly to several proteins of undefined function including Escherichia coli SW:DEDA_ECOLI (EMBL:M68934) DedA hypothetical protein found in the hisT-purF region (219 aa), fasta scores opt: 550 z-score: 642.2 E(): 2.3e-28 43.8% identity in 185 aa overlap and Streptomyces coelicolor TR:Q9X8J1 (EMBL:AL049841) possible membrane protein, SCE9.18c (303 aa), fasta scores opt: 812 z-score: 940.8 E():0 53.7% identity in 227 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a Pfam match to entry PF00597 DedA, DedA family. 4 2 5 4 [Reporter]SCO0592 (18L11)_PCR/3619 [Gene]SCO0592/3289 NC_003888 SCF55.16c, unknown, len: 240 aa 4 3 21 17 [Reporter]SCO2193 (6P14)_PCR/5999 [Gene]SCO2193/5461 NC_003888 SC5F7.08, lipB, probable lipoate-protein ligase, len: 265aa; similar to many eg. SW:LIPB_MYCTU probable lipoate-protein ligase from Mycobacterium tuberculosis (230 aa) fasta scores; opt: 559, z-score: 652.0, E(): 5.5e-29, (52.0% identity in 223 aa overlap) and SW:LIPB_ECOLI lipoate-protein ligase B from Escherichia coli (191 aa) fasta scores; opt: 145, z-score: 177.0, E(): 0.016, (28.9% identity in 225 aa overlap). Contains PS01313 Lipoate-protein ligase B signature. 4 2 5 3 [Reporter]SCO4882 (19H11)_PCR/3618 [Gene]SCO4882/3288 NC_003888 2SCK8.08, unknown, len: 355 aa 4 3 21 16 [Reporter]SCO1415 (7L14)_PCR/5998 [Gene]SCO1415/5460 NC_003888 SC6D7.24c, possible membrane protein, len: 582 aa. Highly similar to the Streptomyces coelicolor hypothetical protein SCF37.26c (64 aa), fasta scores opt: 207 z-score: 284.7 E(): 1.7e-10 52.4% identity in 63 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 5 2 [Reporter]SCO1719 (20P7)_PCR/3617 [Gene]SCO1719/3287 NC_003888 SCI11.08, possible ABC-transporter ATP-binding protein, len: 332 aa; similar to members of the ABC-transporter superfamily e.g. SW:DRRA_STRPE (EMBL:M73758), drrA, Streptomyces peucetius daunorubicin resistance ATP-binding protein (330 aa), fasta scores; opt: 901 z-score: 964.0 E(): 0, 50.6% identity in 316 aa overlap. Similar to many from S.coelicolor e.g. SCE9.25c (EMBL:AL049841) S.coelicolor probable ABC transporter ATP-binding component (315 aa) (57.1% identity in 308 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and PS00017 ATP/GTP-binding site motif A (P-loop) 4 2 4 22 [Reporter]SCO4194 (1P3)_PCR/3615 [Gene]SCO4194/3286 NC_003888 2SCD46.08, possible TetR-family transcriptional regulator, len: 213 aa; similar to many others from streptomycetes e.g. TR:CAB88457 (EMBL:AL353815) putative TetR-family transcriptional regulator from Streptomyces coelicolor (215 aa) fasta scores; opt: 369, z-score: 450.8, E(): 1.3e-17, 37.3% identity in 212 aa overlap and SW:P39885 (TCMR_STRGA) tetracenomycin c transcriptional repressor from Streptomyces glaucescens (226 aa) fasta scores; opt: 246, z-score: 303.8, E(): 2e-09, 36.7% identity in 166 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and helix-turn-helix motif (Score 1437 (+4.08 SD)) at residues 40..61 4 2 4 21 [Reporter]SCO5723 (2L3)_PCR/3614 [Gene]SCO5723/3285 NC_003888 SC3C3.09c, possible regulator, BldB, len: 98 aa; identical to TR:Q53842 (EMBL:U28930) bldB (98 aa) and similar to S. coelicolor TR:Q53896 (EMBL:X60316) AbaA ORFD (75 aa), fasta scores; opt: 150 z-score: 201.9 E(): 0.00055, 47.4% identity in 57 aa overlap 4 2 4 20 [Reporter]SCO5443 (3H3)_PCR/3613 [Gene]SCO5443/3284 NC_003888 SC6A11.19c, pep1A, possible alpha-amylase, len: 675 aa; previously sequenced as TR:O54202 (EMBL:AJ001205). Also similar to TRNEW:AAF07898 (EMBL:AF172946) putative glucanase GlgE from Mycobacterium smegmatis (697 aa) fasta scores; opt: 2199, z-score: 2484.5, E(): 0, 53.6% identity in 679 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase 4 2 4 19 [Reporter]SCO6903 (4D3)_PCR/3612 [Gene]SCO6903/3283 NC_003888 SC1B2.09, unknown, len: 266 aa. Weakly similar to Streptomyces coelicolor TR:CAB72355(EMBL:AL138977) hypothetical 27.8 kd protein, SC7F9.02C (259 aa), fasta scores opt: 149 z-score: 163.3 E(): 0.12 30.0% identity in 283 aa overlap. 4 2 4 18 [Reporter]SCO7081 (4P23)_PCR/3611 [Gene]SCO7081/3282 NC_003888 SC3A4.07c, possible phospholipase, len: 395 aa. Similar to several including: Bacillus firmus TR:O66043(EMBL:U88888) cardiolipin synthase, ClS (503 aa), fasta scores opt: 605 z-score: 748.2 E():0 31.8% identity in 377 aa overlap. Contains 2xPfam matches to entry PF00614 PLDc, Phospholipase D. Active site motif. 4 2 4 17 [Reporter]SCO7068 (5L23)_PCR/3610 [Gene]SCO7068/3281 NC_003888 SC4G1.34c, possible secreted protein, len: 183 aa; similar to TR:CAB69767 (EMBL:AL137187) Streptomyces coelicolor putative secreted protein SC7A8.18, 180 aa; fasta scores: opt: 171 z-score: 179.1 E(): 0.017; 32.8% identity in 192 aa overlap. Contains possible N-terminal region signal peptide sequence 4 2 4 16 [Reporter]SCO6729 (6H23)_PCR/3609 [Gene]SCO6729/3280 NC_003888 SC5F2A.12c, putative membrane protein, len: 396 aa; similar to many e.g. TR:O53176 (EMBL:AL021246) hypothetical protein from Mycobacterium tuberculosis (419 aa) fasta scores; opt: 528, z-score: 598.4, E(): 5.1e-26, (41.0% identity in 420 aa overlap). Contains possible hydrophobic membrane spanning region 4 3 21 15 [Reporter]SCO5223 (8H14)_PCR/5997 [Gene]SCO5223/5459 NC_003888 SC7E4.20, probable cytochrome P450, len: 461 aa; similar to SW:CP51_HUMAN (EMBL:U23942) Homo sapiens cytochrome P450 51 (EC 1.14.14.1) Cyp51, 503 aa; fasta scores: opt: 532 z-score: 587.2 E(): 3.1e-25; 25.7% identity in 447 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450 4 3 21 14 [Reporter]SCO5422 (9D14)_PCR/5996 [Gene]SCO5422/5458 NC_003888 SC8F4.26, hypothetical hydrophilic protein, len: 456 aa; contains possible coiled-coil region around residue 250 4 3 21 13 [Reporter]SCO2575 (10P10)_PCR/5995 [Gene]SCO2575/5457 NC_003888 SCC123.13c, doubtful CDS, len: 75 aa. 4 3 21 12 [Reporter]SCO4331 (11L10)_PCR/5994 [Gene]SCO4331/5456 NC_003888 SCD12A.14, hypothetical protein, len: 1042 aa; similar to TR:CAB61192 (EMBL:AL132973) Streptomyces coelicolor hypothetical 93.0 kD protein SCF91.35, 884 aa; fasta scores: opt: 160 z-score: 164.2 E(): 0.11; 26.0% identity in 666 aa overlap 4 3 21 11 [Reporter]SCO4171 (12H10)_PCR/5993 [Gene]SCO4171/5455 NC_003888 SCD66.08, possible secreted protein, len: 250 aa. Contains possible N-terminal region signal peptide sequence and high content in alanine and glycine amino acid residues 4 3 21 10 [Reporter]SCO3249 (13D10)_PCR/5992 [Gene]SCO3249/5454 NC_003888 SCE29.18c, probable acyl carrier protein, len: 81 aa; member of a large family including SW:ACP_HELPY acyl carrier protein from Helicobacter pylori (78 aa) fasta scores; opt: 121, z-score: 179.0, E(): 0.012, (37.5% identity in 64 aa overlap). Contains Pfam match to entry PF00550 pp-binding, Phosphopantetheine attachment site, score 31.20, E-value 5.3e-07. 4 3 21 9 [Reporter]SCO5043 (14P6)_PCR/5991 [Gene]SCO5043/5453 NC_003888 SCK7.16c, probable hydrolase (putative membrane protein), len: 486 aa; N-terminal similar to SW:APX_STRGR Streptomyces griseus aminopeptidase (EC 3.4.11.-), 284 aa; fasta scores: opt: 967 z-score: 1057.3 E(): 0; 53.7% identity in 283 aa overlap and C-terminal region similar to TR:O69311 (EMBL:D84193) Streptomyces lividans chitinase (EC 3.2.1.14) ChiB, 610 aa; blastp scores: Score = 87 (30.6 bits), Expect = 2.1, P = 0.87; Identities = 46/159 (28%), Positives = 60/159 (37%). Contains possible hydrophobic membrane spanning region 4 3 21 8 [Reporter]SCO3486 (15L6)_PCR/5990 [Gene]SCO3486/5452 NC_003888 SCE65.22, probable aldehyde dehydrogenase, len: 492 aa; similar to SW:ALDA_ECOLI (EBML:M64541) Escherichia coli aldehyde dehydrogenase A (EC 1.2.1.22) aldA, 478 aa; fasta scores: opt: 1189 z-score: 1347.0 E(): 0; 39.1% identity in 473 aa overlap and to Streptomyces coelicolor SCI35.34c, 483 aa; fasta scores: opt: 1014 z-score: 940.7 E(): 0; 40.2% identity in 455 aa overlap. Contains match to Pfam entry PF00171 aldedh, Aldehyde dehydrogenase and two matches to Prosite entries PS00687 Aldehyde dehydrogenases glutamic acid active site PS00070 Aldehyde dehydrogenases cysteine active site 4 2 4 15 [Reporter]SCO7439 (7D23)_PCR/3608 [Gene]SCO7439/3279 NC_003888 SC6D11.35, unknown, len: 246 aa. Weakly similar to a protein of undefined function from Bacillus subtilis TR:P71089(EMBL:Z82044) (251 aa), fasta scores opt: 424 z-score: 507.0 E(): 8e-21 35.4% identity in 209 aa overlap 4 3 21 7 [Reporter]SCO3693 (16H6)_PCR/5989 [Gene]SCO3693/5451 NC_003888 SCH35.31c, unknown, len: 180aa; similar to TR:O33344 (EMBL:AL008883) hypothetical protein from Mycobacterium tuberculosis (194 aa) fasta scores; opt: 266, z-score: 329.4, E(): 5.1e-11, (34.6% identity in 179 aa overlap). Contains TTA codon, possible target for bldA regulation. 4 2 4 14 [Reporter]SCO7175 (8P19)_PCR/3607 [Gene]SCO7175/3278 NC_003888 SC8A11.03, possible regulator, len: 156 aa; similar to TR:Q9S2F7 (EMBL:AL096852) Streptomyces coelicolor putative regulator SCE19A.24, 142 aa; fasta scores: opt: 272 z-score: 353.0 E(): 3.4e-12; 36.9% identity in 130 aa overlap 4 3 21 6 [Reporter]SCO1234 (17D6)_PCR/5988 [Gene]SCO1234/5450 NC_003888 2SCG1.09c, ureC, urease alpha subunit, len: 573 aa; similar to SW:URE1_MYCTU (EMBL:L41141) Mycobacterium tuberculosis urease alpha subunit (EC 3.5.1.5) UreC, 577 aa; fasta scores: opt: 2744 z-score: 2925.3 E(): 0; 67.8% identity in 577 aa overlap and to TR:CAA19974 (EMBL:AL031124) Streptomyces coelicolor putative urease alpha subunit SC1C2.07, 558 aa; fasta scores: opt: 1483 z-score: 1444.2 E(): 0; 46.1% identity in 570 aa overlap. Contains Pfam match to entry PF00449 urease, Urease and match to Prosite entry PS01120 Urease nickel ligands signature and PS00145 Urease active site 4 2 4 13 [Reporter]SCO6447 (9L19)_PCR/3606 [Gene]SCO6447/3277 NC_003888 SC9B5.14c, possible NAD(P)H oxidoreductase, len: 246aa; similar to many (prokaryotic and eukaryotic) eg. SW:DHQV_HUMAN NAD(P)H dehydrogenase from Homo sapiens (human) (231 aa) fasta scores; opt: 182, z-score: 338.0, E(): 1.6e-11, (30.9% identity in 181 aa overlap). 4 2 4 12 [Reporter]SCO2300 (10H19)_PCR/3605 [Gene]SCO2300/3276 NC_003888 SCC30.08c, hypothetical protein, len: 196 aa; similar to SW:Y01A_MYCTU (EMBL:Z70692) Mycobacterium tuberculosis hypothetical 26.9 kD protein MTCY427.10c, 245 aa; fasta scores: opt: 375 z-score: 404.1 E(): 4.6e-15; 37.5% identity in 176 aa overlap. Contains possible colied-coil region at aprox residues 56..112 4 2 4 11 [Reporter]SCO4587 (11D19)_PCR/3604 [Gene]SCO4587/3275 NC_003888 SCD20.05c, hypothetical protein, len: 200 aa; similar to TR:P96726 (EMBL:Z92952) Bacillus subtilis hypothetical 20.5 kDa protein YwqN, 181 aa; fasta scores: opt: 357 z-score: 457.0 E(): 5.5e-18; 33.9% identity in 177 aa overlap 4 2 4 10 [Reporter]SCO3300 (12P15)_PCR/3603 [Gene]SCO3300/3274 NC_003888 SCE15.17, possible secreted serine-rich protein, len: 320aa; contains possible N-terminal signal sequence. 4 2 4 9 [Reporter]SCO3660 (13L15)_PCR/3602 [Gene]SCO3660/3273 NC_003888 SCH10.38c, hypothetical protein, len: 547 aa; unknown function, N-terminus similar to TR:O69933 (EMBL:AL023861), SC3C8.24, Streptomyces coelicolor putative DNA recombinase (672 aa), fasta scores; opt: 192 z-score: 225.8 E(): 2.9e-05, 24.5% identity in 498 aa overlap. Weakly similar to many resolvases. Has unusally low percentage G+C content. Weakly similar to TR:O69933 (EMBL:AL023861) SC3C8.24, Streptomyces coelicolor putative DNA recombinase (672 aa) (24.5% identity in 498 aa overlap) 3 1 9 18 [Reporter]SCO6384 (5C8)_PCR/1201 [Gene]SCO6384/1099 NC_003888 SC3C8.03c, probable integral membrane lysyl-tRNA synthetase, len: 589 aa; similar to SW:P94974 (SYK2_MYCTU) probable lysyl-tRNA synthetase MTCY06H11.04C (1172 aa), fasta scores; opt: 1426, z-score: 1642.9, E(): 0, 44.6% identity in 585 aa overlap. Contains TTA leucine codon; possible target for action of bldA 4 2 4 8 [Reporter]SCO0386 (14H15)_PCR/3601 [Gene]SCO0386/3272 NC_003888 SCF62.12, putative asparagine synthetase, len: 644 aa. Highly similar to many e.g. Mycobacterium tuberculosis SW:ASNH_MYCTU (EMBL; Z70283) putative asparagine synthetase (glutamine-hydrolysing) (EC 6.3.5.4) (652 aa), fasta scores opt: 752 z-score: 874.8 E():0 32.8% identity in 655 aa overlap. Contains a Pfam match to entry PF00310 GATase_2, Glutamine amidotransferases class-II. 3 1 9 17 [Reporter]SCO6677 (6O4)_PCR/1200 [Gene]SCO6677/1098 NC_003888 SC5A7.27c, probable ABC transporter ATP-binding protein, len: 180 aa; similar to part of many e.g. taurine transport atp-binding protein tauB (255 aa), fasta scores; opt: 309 z-score: 436.0 E(): 4.9e-17, 43.6% identity in 117 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporters, score 31.20, E-value 1.9e-09. Has large overlap with convergent downstream gene; database similarities do not extend into the overlap. The sequence of the cosmid has been checked, and is correct 4 2 4 7 [Reporter]SCO1788 (15D15)_PCR/3600 [Gene]SCO1788/3271 NC_003888 SCI51.28c, conserved hypothetical protein, len: 343 aa; unknown function, similar to other hypothetical proteins e.g. TR:O33194 (EMBL:Z98268) Mycobacterium tuberculosis hypothetical protein (353 aa), fasta scores; opt: 768 z-score: 825.7 E(): 0, 44.8% identity in 315 aa overlap, with SCI51.28c. Also shows weak similarity to SW:NAGD_ECOLI (EMBL:X14135), nagD, a CDS in the Escherichia coli N-acetylglucosamine operon (250 aa) (26.7% identity in 243 aa overlap) and to SW:PNPP_SCHPO (EMBL:X62722) Schizosaccharomyces pombe 4-nitrophenylphosphatase (unknown physiological substrate) (269 aa) (27.7% identity in 238 aa overlap) 3 1 9 16 [Reporter]SCO3620 (7K4)_PCR/1199 [Gene]SCO3620/1097 NC_003888 SC66T3.31, possible membrane protein, len: 254 aa; unknown function, contains hyrophobic, possible membrane-spanning region 4 2 4 6 [Reporter]SCO0175 (16P11)_PCR/3599 [Gene]SCO0175/3270 NC_003888 SCJ1.24c, unknown, len: 115 aa. 3 1 9 15 [Reporter]SCO4732 (8G4)_PCR/1198 [Gene]SCO4732/1096 NC_003888 SC6G4.10c, putative membrane protein, len: 299 aa; contains possible transmembrane domain near N-terminus 3 1 9 14 [Reporter]SCO7714 (9C4)_PCR/1197 [Gene]SCO7714/1095 NC_003888 SC8D11.05, possible acetyltransferase, len: 160 aa; low similarity to TR:Q9ZF48 (EMBL:AJ000733) Bacillus megaterium hypothetical 18.7 kDa protein, 160 aa; fasta scores: opt: 159 z-score: 209.7 E(): 0.00038; 26.0% identity in 154 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 3 1 9 13 [Reporter]SCO6472 (9O24)_PCR/1196 [Gene]SCO6472/1094 NC_003888 SC9C7.08c, meaA, coenzyme B12-dependent mutase, len: 675aa; similar to many, very similar to TR:O33614 (EMBL:AF008569) coenzyme B12-dependent mutase from Streptomyces collinus (674 aa) fasta scores; opt: 4085, z-score: 5046.6, E(): 0, (91.7% identity in 675 aa overlap). 3 1 9 12 [Reporter]SCO2692 (10K24)_PCR/1195 [Gene]SCO2692/1093 NC_003888 SCC61A.13, possible repetetive protein, len: 626 aa; contains eleven (weak) Pfam matches to entry PF00400 WD40, WD domain, G-beta repeat and three Prosite matches to PS00678 Trp-Asp (WD) repeats signature. 3 1 9 11 [Reporter]SCO4690 (11G24)_PCR/1194 [Gene]SCO4690/1092 NC_003888 SCD31.15, possible membrane protein, len: 80 aa; similar to N-terminal region of TR:Q9WX28 (EMBL:AL079345) Streptomyces coelicolor putative membrane protein SCE68.03, 441 aa; fasta scores: opt: 237 z-score: 347.7 E(): 6.2e-12; 52.9% identity in 68 aa overlap. Contains possible hydrophobic membrane spanning region 3 1 9 10 [Reporter]SCO3505 (12C24)_PCR/1193 [Gene]SCO3505/1091 NC_003888 SCE134.06c, possible secreted substrate binding protein, len: 429aa; weak similarity to many egs. TR:O87856 (EMBL:AL031013) putative secreted sugar binding protein from Streptomyces coelicolor (438 aa) fasta scores; opt: 368, z-score: 408.8, E(): 1.9e-15, (26.2% identity in 428 aa overlap) and SW:MALE_ECOLI maltose-binding protein from Escherichia coli (396 aa) fasta scores; opt: 188, z-score: 211.5, E(): 0.00018, (23.0% identity in 374 aa overlap). Contains probable N-terminal signal sequence and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 3 1 9 9 [Reporter]SCO0350 (13O20)_PCR/1192 [Gene]SCO0350/1090 NC_003888 SCF41.09, unknown, len: 334 aa. Similar to several hypotheticals including: Rhizobium sp. SW:Y4MH_RHISN (EMBL; AE000085) hypothetical 33.9 KD protein Y4MH (297 aa), fasta scores opt: 262 z-score: 309.5 E(): 6.9e-10 23.8% identity in 303 aa overlap 4 3 21 5 [Reporter]SCO1114 (18P2)_PCR/5987 [Gene]SCO1114/5449 NC_003888 2SCG38.07, ung, uracil DNA glycosylase, len: 225 aa; similar to SW:UNG_STRPN (EMBL:X55651) Streptococcus pneumoniae uracil DNA glycosylase (EC 3.2.2.-) Ung, 217 aa; fasta scores: opt: 519 z-score: 609.6 E(): 1.6e-26; 41.7% identity in 211 aa overlap. Contains Pfam match to entry PF00315 UNG, Uracil-DNA glycosylase and match to Prosite entry PS00130 Uracil-DNA glycosylase signature 4 3 21 4 [Reporter]SCO6104 (19L2)_PCR/5986 [Gene]SCO6104/5448 NC_003888 SCBAC1A6.28c, possible secreted protein, len: 437 aa. Contains possible N-terminal region signal peptide sequence 4 3 21 2 [Reporter]SCO7708 (20P22)_PCR/5984 [Gene]SCO7708/5447 NC_003888 SCBAC12C8.09c, conserved hypothetical protein, len: 216aa: similar to many eg. TR:O86609 (EMBL:AL031155) hypothetical protein from Streptomyces coelicolor (211 aa) fasta scores; opt: 764, Z-score: 865.8, 54.673% identity (55.981% ungapped) in 214 aa overlap. 4 3 20 22 [Reporter]SCO1344 (1P18)_PCR/5982 [Gene]SCO1344/5446 NC_003888 2SCG61.26c, possible solute binding transport lipoprotein, len: 530aa; similar to many eg. TR:Q9RJ53 (EMBL:AL132648) putative lipoprotein oligopeptide binding protein (534 aa) fasta scores; opt: 1438, z-score: 1620.4, E(): 0, 43.4% identity in 539 aa overlap and SW:P76128 (YDDS_ECOLI) putative binding protein YddS precursor (516 aa) fasta scores; opt: 532, z-score: 600.2, E(): 5.9e-26, 25.7% identity in 517 aa overlap. Contains two Pfam match to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5 and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 4 3 20 21 [Reporter]SCO5791 (2L18)_PCR/5981 [Gene]SCO5791/5445 NC_003888 SC4H2.12, miaA, probable tRNA delta(2)-isopentenylpyrophosphate transferase, len: 312 aa; similar to many e.g. SW:MIAA_ECOLI (EMBL:P16384) Escherichia coli tRNA delta(2)-isopentenylpyrophosphate transferase (EC 2.5.1.8) (316 aa), fasta scores; opt: 627 z-score: 781.4 E(): 0, 39.9% identity in 286 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 4 3 20 20 [Reporter]SCO7652 (3H18)_PCR/5980 [Gene]SCO7652/5444 NC_003888 SC10F4.25, possible acetyltransferase, len: 183 aa; similar to TR:Q9Z576 (EMBL:AL035569) Streptomyces coelicolor putative acetyltransferase SC8D9.15, 194 aa; fasta scores: opt: 640 z-score: 820.9 E(): 0; 51.4% identity in 181 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 4 3 20 19 [Reporter]SCO5606 (4D18)_PCR/5979 [Gene]SCO5606/5443 NC_003888 SC2E1.23c, unknown prophage gene, len: 96 aa 4 3 20 18 [Reporter]SCO7679 (5P14)_PCR/5978 [Gene]SCO7679/5442 NC_003888 SC4C2.14, possible transport system integral membrane protein, len: 284 aa; similar to SW:Y4TQ_RHISN (EMBL:Z68203) Rhizobium sp. probable peptide ABC transporter permease protein Y4tQ, 291 aa; fasta scores: opt: 433 z-score: 466.4 E(): 1.9e-18; 33.2% identity in 277 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 4 5 [Reporter]SCO5013 (17L11)_PCR/3598 [Gene]SCO5013/3269 NC_003888 SCK15.15, possible secreted protein, len: 198 aa. Contains possible N-terminal region signal peptide sequence. High content in alanine amino acid residues 4 3 20 17 [Reporter]SCO6749 (6L14)_PCR/5977 [Gene]SCO6749/5441 NC_003888 SC5F2A.32, unknown, len: 161 aa; similar to other hypothetical proteins egs. TR:O54111 (EMBL:AL021529) from Streptomyces coelicolor (171 aa) fasta scores; opt: 221, z-score: 281.8, E(): 2.2e-08, (35.8% identity in 137 aa overlap) and TR:O69204 (EMBL:U33059) Actinosynnema pretiosum auranticum (144 aa) fasta scores; opt: 220, z-score: 281.8, E(): 2.2e-08, (38.7% identity in 155 aa overlap). 4 2 4 3 [Reporter]SCO5350 (19D11)_PCR/3596 [Gene]SCO5350/3268 NC_003888 SCBAC5H2.19, unknown, len: 181 aa. Contains two TTA leucine codons, possible targets for bldA regulation 4 3 20 15 [Reporter]SCO5219 (8D14)_PCR/5975 [Gene]SCO5219/5440 NC_003888 SC7E4.16, possible lipoprotein, len: 418 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site, possible N-terminal region signal peptide sequence and possible hydrophobic membrane spanning regions 4 2 3 22 [Reporter]SCO4190 (1L3)_PCR/3593 [Gene]SCO4190/3267 NC_003888 2SCD46.04, possible GntR-family transcriptional regulator, len: 291aa; similar to many eg. TR:Q9RIQ2 (EMBL:AJ243257) KorA protein from plasmid pSNA1 of Streptomyces natalensis (245 aa) fasta scores; opt: 175, z-score: 209.9, E(): 0.00033, 29.9% identity in 194 aa overlap. Also strongly similar to neighbouring CDS 2SCD46.02 fasta scores; opt: 1074, z-score: 984.2, E(): 0, 57.6% identity in 302 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and helix-turn-helix motif (Score 2173 (+6.59 SD)) at residue 41-62. Note possible alternative downstream translational start codons. 4 2 3 21 [Reporter]SCO0769 (2H3)_PCR/3592 [Gene]SCO0769/3266 NC_003888 3SCF60.01c, probable oxidoreductase (fragment), len: >53 aa; similar to SW:YAJO_ECOLI (EMBL:AE000148) Escherichia coli hypothetical oxidoreductase in pgpA-ispA intergenic region YajO, 324 aa; fasta scores: opt: 250 z-score: 383.7 E(): 7.6e-14; 65.4% identity in 52 aa overlap,SCF81.28c, probable aldo/keto reductase (partial CDS), len: >307 aa; similar to TR:AAD30468 (EMBL:AF124928) Streptomyces clavuligerus putative aldo/keto reductase family 2 enzyme Cvm1, 344 aa; fasta scores: opt: 521 z-score: 608.2 E(): 1.7e-26; 35.3% identity in 323 aa overlap and to SW:NORA_ASPFL (EMBL:U32377) Aspergillus flavus Norsolorinic acid reductase (EC 1.1.1.-) NorA, 388 aa; fasta scores: opt: 562 z-score: 654.8 E(): 4.3e-29; 33.7% identity in 303 aa overlap. Contains match to Pfam entry PF00248 aldo_ket_red, Aldo/keto reductase family 4 2 3 19 [Reporter]SCO6145 (3P23)_PCR/3590 [Gene]SCO6145/3265 NC_003888 SC1A9.09, unknown, len: 572 aa; Contains His rich extreme N-terminal. 4 2 3 18 [Reporter]SCO2032 (4L23)_PCR/3589 [Gene]SCO2032/3264 NC_003888 SC4G6.01, partial CDS, unknown, len: >35 aa,SC3A3.10, hypothetical protein, len: 404 aa; unknown function, similar to both bacterial dehydratases e.g. SW:CAIB_ECOLI (EMBL:X67748) Escherichia coli L-carnitine dehydratase (405 aa), fasta scores; opt: 579 z-score: 647.7 E(): 1e-28, 28.9% identity in 409 aa overlap and to eukaryotic isomerases e.g. TR:O43673 (EMBL:AF047020) Homo sapiens alpha-methylacyl-CoA racemase (382 aa) (31.2% identity in 398 aa overlap). Similar to others from S.coelicolor e.g. SC5F2A.13 (EMBL:AL049587) S.coelicolor possible racemase (398 aa) (35.9% identity in 404 aa overlap). Present, in part, in cosmid 4G6 (EMBL:AL096884) as SC4G6.01 4 2 3 17 [Reporter]SCO6555 (5H23)_PCR/3588 [Gene]SCO6555/3263 NC_003888 SC4B5.05c, LysR-family transcriptional regulator, len: 295 aa; similar to several members of the LysR family of transcriptional regulators eg. TR:G3273739 (EMBL:AF057064) HexA from Erwinia carotovora subspecies atroseptica (316 aa), fasta scores; opt: 453, z-score: 562.2, E(): 5.1e-24, (32.6% identity in 276 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature, PS00287 Cysteine proteases inhibitors signature and Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix proteins, lysR family, score 143.70, E-value 3.3e-39. Contains strong helix-turn-helix motif (score 2054, +6.18 SD) at residue18 10 39. 3 1 9 8 [Reporter]SCO3348 (14K20)_PCR/1191 [Gene]SCO3348/1089 NC_003888 SCE7.15c, possible DNA-binding protein, len: 309 aa; similar to SW:YV22_MYCTU hypothetical protein from Mycobacterium tuberculosis (328 aa) fasta scores; opt: 991, z-score: 1143.8, E(): 0, (55.0% identity in 289 aa overlap) and similar to N-terminal region of SW:PPX_ECOLI (EMBL:L06129) Escherichia coli exopolyphosphatase Ppx, 512 aa; fasta scores: opt: 286 Z-score: 317.1 E(): 5e-10; 25.329% identity in 304 aa overlap. Contains possible helix-turn-helix motif (+3.03 SD) at 251..272 aa 4 2 3 16 [Reporter]SCO6724 (6D23)_PCR/3587 [Gene]SCO6724/3262 NC_003888 SC5F2A.07, unknown, len: 109 aa 3 1 9 7 [Reporter]SCO2874 (15G20)_PCR/1190 [Gene]SCO2874/1088 NC_003888 SCE6.11c, possible oxidoreductase, len: 323 aa. Similar to several other members of the short-chain dehydrogenases/reductases family including: Streptomyces antibioticus SW:OXIR_STRAT(EMBL:M96551) probable oxidoreductase (298 aa), fasta scores opt: 641 z-score: 722.8 E(): 7.9e-33 43.4% identity in 304 aa overlap and Streptomyces coelicolor TR:AAF26372(EMBL:AF204735) putative NADP-dependent oxidoreductase (297 aa), fasta scores opt: 670 z-score: 755.1 E(): 0 43.9% identity in 289 aa overlap. Contains a Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 4 2 3 14 [Reporter]SCO7600 (8L19)_PCR/3585 [Gene]SCO7600/3261 NC_003888 SC7H9.12c, possible alanyl tRNA synthetase, len: 404 aa; similar to N-terminal region of SW:SYA_THIFE (EMBL:X95571) Thiobacillus ferrooxidans alanyl-tRNA-synthetase (EC 6.1.1.7) AlaS, 877 aa; fasta scores: opt: 728 z-score: 825.4 E(): 0; 37.4% identity in 425 aa overlap. Contains Pfam match to entry PF01411 tRNA-synt_2c, tRNA synthetases class II (A) and matches to Prosite entries PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2 and PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 9 6 [Reporter]SCO1656 (16C20)_PCR/1189 [Gene]SCO1656/1087 NC_003888 SCI46.01c, possible hydrolase, len: 233 aa; similar to TR:CAC13072 (EMBL:AL445503) Streptomyces coelicolor putative hydrolase 2SCG38.13, 238 aa; fasta scores: opt: 323 z-score: 377.6 E(): 1.7e-13; 36.6% identity in 191 aa overlap. Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase 4 2 3 13 [Reporter]SCO7158 (9H19)_PCR/3584 [Gene]SCO7158/3260 NC_003888 SC9A4.20, conserved hypothetical protein, len: 812 aa; similar to TR:Q9RJC6 (EMBL:AL133171) Streptomyces coelicolor hypothetical 89.7 kDa protein SCF81.26, 835 aa; fasta scores: opt: 1262 z-score: 1348.6 E(): 0; 37.3% identity in 808 aa overlap. Contains 2x Pfam matches to entry PF00989 PAS, PAS domain, Pfam match to PF00785 PAC, PAC motif and match to Prosite entry PS00213 Lipocalin signature 3 1 9 5 [Reporter]SCO3859 (17O16)_PCR/1188 [Gene]SCO3859/1086 NC_003888 SCH69.29, possible DNA-binding protein, len: 182 aa; highly similar to TR:CAA09629 (EMBL:AJ011500) hypothetical protein from granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22 (182 aa) fasta scores; opt: 1045, z-score: 1158.8, E(): 0, (90.1% identity in 182 aa overlap). Contains probable helix-turn-helix 38-59aa (+3.20 SD). 3 1 9 4 [Reporter]SCP1.243 (18K16)_PCR/1187 [Gene]SCP1.243/1085 NC_003888 SCP1.243, mmfL, possible lactone biosynthesis protein, len: 353aa; previously sequenced and annotated as hypothetical protein TR:Q9JN90 (EMBL:AJ276673). Similar to many others eg. TR:O86851 (EMBL:AJ007731) ScbA gamma-butyrolactone biosynthesis protein from Streptomyces coelicolor (314 aa) fasta scores; opt: 336, z-score: 389.2, E(): 3.3e-14, 28.7% identity in 310 aa overlap. Contains a TTA encoded leucine at residue 8, possible target for bldA regulation. 3 1 9 3 [Reporter]SCO7465 (19G16)_PCR/1186 [Gene]SCO7465/1084 NC_003888 SCBAC14E8.05, cvnC13, hypothetical protein, len: 125 aa; similar to TR:Q9X834 (EMBL:AL049727) Streptomyces coelicolor hypothetical 12.8 kDa protein SC9B1.14c, 119 aa; fasta scores: opt: 288 Z-score: 370.8 bits: 74.1 E(): 4.5e-13; 45.082% identity in 122 aa overlap. Contains a TTA leucine codon, possible target for bldA regulation 3 1 9 2 [Reporter]SCO2824 (20O12)_PCR/1185 [Gene]SCO2824/1083 NC_003888 SCBAC17F8.15, possible NAD-binding protein, len: 327aa: similar to many predicted dehydrogenases eg. TR:Q9KEA4 (EMBL:AP001510) D-3-phosphoglycerate dehydrogenase from Bacillus halodurans (316 aa) fasta scores; opt: 523, Z-score: 556.0, 33.808% identity (35.055% ungapped) in 281 aa overlap. Contains Pfam match to entry PF02826 2-Hacid_DH_C, D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain but does not contain the corresponding catalytic domain. 3 1 8 22 [Reporter]SCO1238 (1O8)_PCR/1183 [Gene]SCO1238/1082 NC_003888 2SCG1.13, probable ATP-dependent Clp protease, len: 211 aa; similar to TR:Q9ZH58 (EMBL:AF071885) Streptomyces coelicolor ATP-dependent Clp protease proteolytic subunit 2 (EC 3.4.21.92) ClpP2, 236 aa; fasta scores: opt: 769 z-score: 885.2 E(): 0; 55.8% identity in 208 aa overlap. Contains Pfam match to entry PF00574 CLP_protease, Clp protease 3 1 8 21 [Reporter]SCO0191 (2K8)_PCR/1182 [Gene]SCO0191/1081 NC_003888 SCJ12.03c, crtY, probable lycopene cyclase, len: 405 aa. Highly similar to Streptomyces griseus TR:P72451 (EMBL:X95596) lycopene cyclase (418 aa), fasta scores opt: 1426 z-score: 1602.7 E(): 0 56.3% identity in 396 aa overlap 3 1 8 20 [Reporter]SCO4140 (3G8)_PCR/1181 [Gene]SCO4140/1080 NC_003888 SCD84.07c, pstA, phosphate ABC transport system permease protein, len: 354 aa; similar to TR:O86345 (EMBL:X75297) Mycobacterium tuberculosis phosphate transport system permease protein PstA1 aa; fasta scores: opt: 870 z-score: 1011.0 E(): 0; 48.4% identity in 281 aa overlap and to SW:PSTA_ECOLI (EMBL:X02723) Escherichia coli phosphate transport system permease protein PstA or PhoT, 296 aa; fasta scores: opt: 605 z-score: 705.5 E(): 7.7e-32; 37.2% identity in 277 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 4 3 20 13 [Reporter]SCO2569 (10L10)_PCR/5973 [Gene]SCO2569/5439 NC_003888 SCC123.07c, unknown, len: 281 aa. Similar to several proteins of undefined function including: Bacillus subtilis SW:DEGV_BACSU(EMBL:Z18629) DegV protein (281 aa), fasta scores opt: 549 z-score: 626.7 E(): 1.6e-27 37.1% identity in 278 aa overlap and Mycobacterium tuberculosis TR:P71726 (EMBL:Z81368) hypothetical 28.5 KD protein (280 aa), fasta scores opt: 579 z-score: 660.5 E(): 2.1e-29 38.3% identity in 274 aa overlap 4 3 20 12 [Reporter]SCO2497 (11H10)_PCR/5972 [Gene]SCO2497/5438 NC_003888 SCC82.03c, hypothetical protein, len: 406 aa; similar to varous hypothetical proteins, e.g. SW:Y823_MYCTU (EMBL:AL022004) Mycobacterium tuberculosis hypothetical 41.4 kD protein RV0823c, 389 aa; fasta scores: opt: 1153 z-score: 1349.3 E(): 0; 56.2% identity in 406 aa overlap. Contains Pfam match to entry PF01207 UPF0034, Uncharacterized protein family UPF0034 and match to Prosite entry PS01136 Uncharacterized protein family UPF0034 signature 4 3 20 10 [Reporter]SCO3276 (13P6)_PCR/5970 [Gene]SCO3276/5437 NC_003888 SCE39.26, possible integral membrane transporter, len: 499aa; similar to many eg. TR:Q50443 (EMBL:U50335) ORF2 from Mycobacterium smegmatis (498 aa) fasta scores; opt: 2027, z-score: 2225.4, E(): 0, (64.0% identity in 478 aa overlap) and TR:O07488 (EMBL:Y13308) proposed sulphate permease from Yersinia enterocolitica (492 aa) fasta scores; opt: 1564, z-score: 1718.0, E(): 0, (51.0% identity in 482 aa overlap). Contains Pfam match to entry PF00916 Sulfate_transp, Sulfate transporter family. Also contains several possible membrane spanning hydrophobic regions. 4 3 20 9 [Reporter]SCO0695 (14L6)_PCR/5969 [Gene]SCO0695/5436 NC_003888 SCF42.05, unknown, len: 328 aa; similar to TR:Q9KZT0 (EMBL:AL353832) hypothetical protein SCE6.08 from Streptomyces coelicolor (326 aa) fasta scores; opt: 371, Z-score: 407.1, E(): 4.8e-15, 35.629% identity (39.145% ungapped) in 334 aa overlap. 4 3 20 8 [Reporter]SCO4839 (15H6)_PCR/5968 [Gene]SCO4839/5435 NC_003888 SC5G8.07, trpS2, tryptophanyl-tRNA synthetase, len: 339aa; strongly similar to many eg. SW:P00953 (SYW_BACST) tryptophanyl-tRNA synthetase from Bacillus stearothermophilus (328 aa) fasta scores; opt: 1195, z-score: 1347.2, E(): 0, 55.7% identity in 325 aa overlap. Also similar to TR:Q9X8F8 (EMBL:AL049819) trpS, tryptophanyl-tRNA synthetase (fragment) from Streptomyces coelicolor (208 aa) fasta scores; opt: 618, z-score: 703.4, E(): 1e-31, 50.5% identity in 204 aa overlap.Contains Pfam match to entry PF00579 tRNA-synt_1b, tRNA synthetases class I (W and Y) and Prosite match to PS00178 Aminoacyl-transfer RNA synthetases class-I signature. 4 3 20 7 [Reporter]SCO1625 (16D6)_PCR/5967 [Gene]SCO1625/5434 NC_003888 SCI41.08, probable ribosomal pseudouridine synthase, len: 324 aa. Similar to SW:RLUA_ECOLI (EMBL:D10483) Escherichia coli ribosomal large subunit pseudouridine synthase 218 aa; fasta scores: opt: 262 z-score: 302.0 E(): 1.9e-09; 31.7% identity in 221 aa overlap. Contains Pfam match to entry PF00849 YABO, Hypothetical yabO/yceC/sfhB family also PS01129 Rlu family of pseudouridine synthase signature 4 3 20 6 [Reporter]SCO1916 (17P2)_PCR/5966 [Gene]SCO1916/5433 NC_003888 SCI7.34c, possible transferase, len: 329 aa; similar to many e.g. TR:BAA75911 (EMBL:AB024601) tetrahydrodipicolinate N-succinyltransferase from Pseudomonas aeruginosa (344 aa) fasta scores; opt: 1004, z-score: 1062.7, E(): 0, (54.4% identity in 307 aa overlap). Contains possible N-terminal signal sequence. 4 3 20 5 [Reporter]SCO0385 (18L2)_PCR/5965 [Gene]SCO0385/5432 NC_003888 SCF62.11, possible membrane protein, len: 521 aa. Contains possible membrane spanning hydrophobic domains and two possible coiled-coils from 144 to 182 (39 residues) and from 190 to 221 (32 residues). 4 2 3 12 [Reporter]SCO2295 (10D19)_PCR/3583 [Gene]SCO2295/3259 NC_003888 SCC30.03, hypothetical protein, len: 253 aa; N-terminal region similar to TR:CAB52842 (EMBL:AL109848) Streptomyces coelicolor putative transcriptional regulator SCI51.18, 548 aa; fasta scores: opt: 114 z-score: 134.1 E(): 5; 30.6% identity in 196 aa overlap. Contains possible coiled-coil region at aprox residues 76..93 4 3 20 4 [Reporter]SCP1.236 (19H2)_PCR/5964 [Gene]SCP1.236/5431 NC_003888 SCP1.236, mmyK, possible ATP/GTP-binding protein, len: 206aa; previously sequenced and annotated as TR:Q9JN85 (EMBL:AJ276673). Also similar to neighbouring CDS SCP1.233A MmyX, putative ATP/GTP-binding protein (234 aa) (previously sequenced as TR:Q9JN81 (EMBL:AJ276673) hypothetical protein) fasta scores; opt: 538, z-score: 647.7, E(): 1.3e-28, 42.7% identity in 199 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 2 3 11 [Reporter]SCO4512 (11P15)_PCR/3582 [Gene]SCO4512/3258 NC_003888 SCD35.19c, unknown, len: 193 aa 4 3 20 3 [Reporter]SCP1.149c (20D2)_PCR/5963 [Gene]SCP1.149c/5430 NC_003888 SCP1.149c, unknown, len: 369aa; similar to SCP1.181c (pseudogene) fasta scores; opt: 331, z-score: 405.0, E(): 4.9e-18, 44.2% identity in 120 aa overlap. 4 2 3 10 [Reporter]SCO3296 (12L15)_PCR/3581 [Gene]SCO3296/3257 NC_003888 SCE15.13c, possible oxidoreductase, len: 367aa; some similarity to TR:CAA77275 (EMBL:Y18729) F420-dependent alcoholdehydrogenase from Methanoculleus thermophilicus (330 aa) fasta scores; opt: 211, z-score: 252.,1 E(): 9.9e-07, (29.0% identity in 183 aa overlap). 4 2 3 9 [Reporter]SCO3549 (13H15)_PCR/3580 [Gene]SCO3549/3256 NC_003888 SCH5.12c, bldG, probable anti-sigma factor antagonist, len: 113 aa; similar to many e.g. SW:RSBV_BACSU RsbV, anti-sigma B factor antagonist from Bacillus subtilis (109 aa) fasta scores; opt: 255, z-score: 341.7, E(): 9.9e-12, (45.7% identity in 94 aa overlap) and SW:SP21_BACSU spoIIAA, anti-sigma F factor antagonist (116 aa) fasta scores; opt: 179, z-score: 244.8, E(): 2.5e-06, (30.2% identity in 96 aa overlap). 4 2 3 8 [Reporter]SCO4295 (14D15)_PCR/3579 [Gene]SCO4295/3255 NC_003888 SCD95A.28, scoF4, cold shock protein, len: 67 aa; highly similar to TR:AAD49327 (EMBL:AF162937) Streptomyces coelicolor A3(2) cold shock protein F40, 67 aa; fasta scores: opt: 317 z-score: 454.8 E(): 7.2e-18; 70.3% identity in 64 aa overlap. Contains Pfam match to entry PF00313 CSD, 'Cold-shock' DNA-binding domain and match to Prosite entry PS00352 'Cold-shock' DNA-binding domain signature 4 2 3 7 [Reporter]SCO1911 (15P11)_PCR/3578 [Gene]SCO1911/3254 NC_003888 SCI7.29c, possible integral membrane protein, len: 165aa; similar to others from Mycobacterium tuberculosis and Streptomyces coelicolor egs. TR:O69637 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (172 aa) fasta scores; opt: 194, z-score: 223.8, E(): 3.9e-05, (30.6% identity in 134 aa overlap) and SW:CAA22031 (EMBL:AL033505) putative membrane protein from Streptomyces coelicolor (205 aa) fasta scores; opt: 187, z-score: 215.2, E(): 0.00012, (33.8% identity in 130 aa overlap). Contains possible membrane spanning hydrophobic region. Also contains a helix-turn-helix motif (+3.21 SD) 39-60aa. 4 2 3 6 [Reporter]SCO0237 (16L11)_PCR/3577 [Gene]SCO0237/3253 NC_003888 SCJ9A.16, possible oxidoreductase, len: 253 aa; similar to many proposed oxidoreductases e.g. SW:DLTE_BACSU from Bacillus subtilis (252 aa) fasta scores; opt: 368, z-score: 429.4, E(): 1.5e-16, (33.2% identity in 205 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature 3 1 8 19 [Reporter]SCO5528 (4C8)_PCR/1180 [Gene]SCO5528/1079 NC_003888 SC1C2.09, possible transcriptional regulator, len: 200 aa; some similarity to e.g. TR:O34381 (EMBL:Z99112) transcriptional regulator PksA from Bacillus subtilis (205 aa), fasta scores; opt: 182 z-score: 206.5 E(): 0.0003, 26.5% identity in 185 aa overlap. Contains probable helix-turn-helix motif at aa 32-53 (Score 1136, +3.06 SD) and Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family, score 35.50, E-value 4.1e-08 4 2 3 3 [Reporter]SCO1311 (19P7)_PCR/3574 [Gene]SCO1311/3252 NC_003888 SCBAC36F5.22c, conserved hypothetical protein, len: 185 aa; similar to TR:Q9I1H4 (EMBL:AE004656) Pseudomonas aeruginosa hypothetical protein PA2301, 180 aa; fasta scores: opt: 386 Z-score: 464.0 bits: 92.5 E(): 3.1e-18; 44.118% identity in 170 aa overlap 3 1 8 18 [Reporter]SCO6570 (5O4)_PCR/1179 [Gene]SCO6570/1078 NC_003888 SC3F9.05, possible oxidoreductase, len: 430 aa; similar to e.g. Zymomonas mobilisTR:P75002 (EMBL:Z80356) glucose-fructose oxidoreductase (433 aa), fasta scores; opt: 310 z-score: 305.5 E(): 8.4e-10, 27.2% identity in 426 aa overlap 4 2 3 2 [Reporter]SCO5335 (20H7)_PCR/3573 [Gene]SCO5335/3251 NC_003888 SCBAC5H2.04, unknown, len: 87 aa 3 1 8 17 [Reporter]SCO6673 (6K4)_PCR/1178 [Gene]SCO6673/1077 NC_003888 SC5A7.23, unknown, len: 226 aa; similar to hypothetical proteins from Streptomyces and M. tuberculosis e.g. S. coelicolor TR:O24813 (EMBL:AB007189) (208 aa), fasta scores; opt: 674 z-score: 960.0 E(): 0, 49.1% identity in 214 aa overlap 4 2 2 21 [Reporter]SCO0739 (2D3)_PCR/3570 [Gene]SCO0739/3250 NC_003888 3SC5B7.17c, possible oxidoreductase, len: 308 aa; highly similar to TR:O54118 (EMBL:AL021529) Streptomyces coelicolor putative oxidoreductase SC10A5.27c, 326 aa; fasta scores: opt: 1123 z-score: 1316.4 E(): 0; 60.1% identity in 308 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase 3 1 8 16 [Reporter]SCO7288 (7G4)_PCR/1177 [Gene]SCO7288/1076 NC_003888 SC5H1.04c, possible membrane protein, len: 167 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and the presence of a possible RBS; similar to TR:Q9AJX8 (EMBL:AL512944) Streptomyces coelicolor putative integral membrane protein SC8D11.43c, 184 aa; fasta scores: opt: 505 Z-score: 606.1 E(): 4e-26; 48.667% identity in 150 aa overlap. Contains hydrophobic, possible membrane-spanning regions 3 1 8 15 [Reporter]SCO6688 (8C4)_PCR/1176 [Gene]SCO6688/1075 NC_003888 SC6G3.04, hypothetical protein, len: 1171 aa; unknown function, C-terminal 380 aa (approx.) similar to SW:YEHP_ECOLI (EMBL:AE000301), yehP, Escherichia coli hypothetical protein (378 aa), fasta scores; opt: 610 z-score: 603.7 E(): 2.7e-26, 34.5% identity in 394 aa overlap and N-terminal 750 aa (approx.) similar to SW:YEHM_ECOLI (EMBL:AE000301), yehM, Escherichia coli hypothetical protein (759 aa) (25.4% identity in 796 aa overlap). The hypothetical proteins yehMP are adjacent on the E.coli chromosome. Part of this probable CDS (aa 530-830 approx.) is also similar to TR:Q54218 (EMBL:X77865) Streptomyces griseus putative chain length determination factor (412 aa) (31.5% identity in 340 aa overlap) 3 1 8 14 [Reporter]SCO6593 (8O24)_PCR/1175 [Gene]SCO6593/1074 NC_003888 SC8A6.14c, unknown, len: 1147 aa; contains two Ala-rich domains, PS00017 ATP/GTP-binding site motif A (P-loop) and probable coiled-coil regions from 238 to 292, 399 to 441, 445 to 488, 490 to 573, 786 to 820, and 829 to 871 3 1 8 13 [Reporter]SCO6468 (9K24)_PCR/1174 [Gene]SCO6468/1073 NC_003888 SC9C7.04c, conserved hypothetical protein, len: 218 aa; similar to several hypothetical proteins e.g. TR:O27105 (EMBL:AE000875) Methanobacterium thermoautotrophicum (223 aa) fasta scores; opt: 405, z-score: 712.9, E(): 2e-32, (39.2% identity in 212 aa overlap) 3 1 8 12 [Reporter]SCO2315 (10G24)_PCR/1173 [Gene]SCO2315/1072 NC_003888 SCC53.06, possible membrane protein, len: 97aa; contains possible membrane-spanning hydrophobic regions. 3 1 8 11 [Reporter]SCO4685 (11C24)_PCR/1172 [Gene]SCO4685/1071 NC_003888 SCD31.10, probable DEAD-box RNA helicase, len: 473 aa; similar to TR:O54116 (EMBL:AL021529) Streptomyces coelicolor DEAD-box RNA helicase SC10A5.25c, 498 aa; fasta scores: opt: 2591 z-score: 2737.2 E(): 0; 88.1% identity in 461 aa overlap and to SW:RHLE_ECOLI (EMBL:L02123) Escherichia coli putative ATP-dependent RNA helicase RhlE, 454 aa; fasta scores: opt: 845 z-score: 896.7 E(): 0; 36.8% identity in 427 aa overlap. Contains Pfam match to entry PF00270 DEAD, DEAD/DEAH box helicase Pfam match to entry PF00271 helicase_C, Helicases conserved C-terminal domain 2x PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 8 10 [Reporter]SCO3524 (12O20)_PCR/1171 [Gene]SCO3524/1070 NC_003888 SCE2.05, unknown, len: 221aa; 4 3 20 2 [Reporter]SCO5069 (20L22)_PCR/5962 [Gene]SCO5069/5429 NC_003888 SCBAC20F6.12, possible oxidoreductase, len: 318 aa; similar to many oxidoreductases/dehydratases, e.g. TR:Q9WX20 (EMBL:AL079345) Streptomyces coelicolor putative dehydrogenase SCE68.12c, 318 aa; fasta scores: opt: 998 Z-score: 1062.9 bits: 204.9 E(): 1.3e-51; 52.997% identity in 317 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases 4 3 19 22 [Reporter]SCO1339 (1L18)_PCR/5960 [Gene]SCO1339/5428 NC_003888 2SCG61.21, hypothetical protein, len: 206 aa; similar to TR:Q9X9V5 (EMBL:AL096743) hypothetical protein from Streptomyces coelicolor (197 aa) fasta scores; opt: 212, z-score: 254.4, E(): 1.1e-06, 33.9% identity in 186 aa overlap. 4 3 19 21 [Reporter]SCO4296 (2H18)_PCR/5959 [Gene]SCO4296/5427 NC_003888 SCD95A.29, groEL2, 60 kD chaperonin 2, len: 541 aa; identical to SW:CH62_STRAL (EMBL:M76658) Streptomyces albus g. 60 kD chaperonin 2 GroEL2, 539 aa and highly similar to TR:CAA20418 (EMBL:AL031317) Streptomyces coelicolor 60 kD chaperonin cpn60, groEL1, 541 aa; fasta scores: opt: 2371 z-score: 2327.6 E(): 0; 69.6% identity in 40 aa overlap. Contains Pfam match to entry PF00118 cpn60_TCP1, TCP-1/cpn60 chaperonin family and match to Prosite entry PS00296 Chaperonins cpn60 signature 4 3 19 20 [Reporter]SCO7648 (3D18)_PCR/5958 [Gene]SCO7648/5426 NC_003888 SC10F4.21c, probable two-component system response regulator, len: 221 aa; similar to many other Streptomyces coelicolor response regulators, e.g. TR:Q9RKT8 (EMBL:AL132824) putative response regulator SCAH10.19, 229 aa; fasta scores: opt: 713 z-score: 802.2 E(): 0; 57.2% identity in 208 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Also contains possible helix-turn-helix motif at residues 163..184 (+3.19 SD) 4 3 19 19 [Reporter]SCO7213 (4P14)_PCR/5957 [Gene]SCO7213/5425 NC_003888 SC2H12.12, unknown, len: 248 aa. Similar to Streptomyces coelicolor TR:CAB89463(EMBL:AL354048) putative fatty acid synthase, SCE25.32C (438 aa), fasta scores opt: 195 z-score: 237.4 E(): 9.5e-06 26.3% identity in 232 aa overlap. Also highly similar to Escherichia coli SW:YJHP_ECOLI(EMBL:U14003) hypothetical 27.4 kd protein (248 aa), fasta scores opt: 1174 z-score: 1413.2 E():0 66.5% identity in 248 aa overlap. 4 3 19 17 [Reporter]SCO7766 (6H14)_PCR/5955 [Gene]SCO7766/5424 NC_003888 SC5E9.14, conserved hypothetical protein, len: 296aa; similar to TR:Q53906 (EMBL:X58833) hypothetical protein from the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor (294 aa) fasta scores; opt: 546, z-score: 609.8, E(): 1.7e-26, 39.2% identity in 288 aa overlap 4 3 19 15 [Reporter]SCO6012 (8P10)_PCR/5954 [Gene]SCO6012/5423 NC_003888 SC7B7.09c, probable hydrolase (putative secreted protein), len: 507 aa; similar to many chitinases eg. TR:Q59145 (EMBL:D31818) chitinase II precursor (EC 3.2.1.14) Aeromonas sp. (542 aa), fasta scores; opt: 410 z-score: 512.7 E(): 2.1e-21, 32.1% identity in 498 aa overlap. Contains N-terminal signal sequence and PS00017 ATP/GTP-binding site motif A (P-loop) 4 3 19 14 [Reporter]SCO7003 (9L10)_PCR/5953 [Gene]SCO7003/5422 NC_003888 SC8F11.29c, unknown, len: 336 aa. Similar in parts to a protein of undefined function from Streptomyces coelicolor: TR:CAB46782(EMBL:AL096811) SCI30A.05C (338 aa), fasta scores opt: 249 z-score: 278.0 E(): 4.7e-08 34.8% identity in 319 aa overlap. 4 2 2 20 [Reporter]SCO4565 (2P23)_PCR/3569 [Gene]SCO4565/3249 NC_003888 SCD16A.18c, nuoD, NADH dehydrogenase subunit, len: 440 aa; similar to many e.g. TR:P95178 (EMBL:Z83867) NuoD, NADH dehydrogenase subunit from Mycobacterium tuberculosis (440 aa) fasta scores; opt: 2198, z-score: 2569.2, E(): 0, (73.9% identity in 429 aa overlap) and SW:NUOD_ECOLI, NADH dehydrogenase subunit NuoD from Escherichia coli (407 aa) fasta scores; opt: 808, z-score: 946.5, E(): 0, (40.8% identity in 407 aa overlap). Contains Pfam match to entry PF00346 complex1_49Kd, Respiratory-chain NADH dehydrogenase, 49 Kd subunit. 4 3 19 13 [Reporter]SCO3793 (10H10)_PCR/5952 [Gene]SCO3793/5421 NC_003888 SCAC2.01, conserved hypothetical protein, len: 121 aa; similar to TR:O34593 (EMBL:Z99110) Bacillus subtilis YjqA protein, 125 aa; fasta scores: opt: 459 z-score: 636.3 E(): 6.2e-28; 60.5% identity in 114 aa overlap 4 2 2 19 [Reporter]SCO6141 (3L23)_PCR/3568 [Gene]SCO6141/3248 NC_003888 SC1A9.05c, possible transmembrane protein, len: 200 aa; contains possible membrane spanning hydrophobic regions 4 3 19 12 [Reporter]SCO2623 (11D10)_PCR/5951 [Gene]SCO2623/5420 NC_003888 SCC80.08c, hypothetical protein, len: 164 aa; similar to N-terminal domain of SW:RELA_CORGL (EMBL:AF038651) Corynebacterium glutamicum GTP pyrophosphokinase (EC 2.7.6.5) RelA, 760 aa; fasta scores: opt: 162 z-score: 170.8 E(): 0.054; 33.6% identity in 149 aa overlap. Contains 3x conserved repeat: MPP 4 2 2 18 [Reporter]SCO6198 (4H23)_PCR/3567 [Gene]SCO6198/3247 NC_003888 SC2G5.19, possible secreted protein, len: 1156aa; alanine rich, similar to TR:O87848 (EMBL:AL031013) hypothetical protein from Streptomyces coelicolor (1147 aa) fasta scores; opt: 2516, z-score: 2119.1, E(): 0, (44.6% identity in 1122 aa overlap). Contains possible N-terminal signal sequence. 4 2 2 17 [Reporter]SCO6551 (5D23)_PCR/3566 [Gene]SCO6551/3246 NC_003888 SC4B5.01c, probable oxidoreductase, len; 277aa, similar to many eg. TR:P06632 (EMBL:M12799) 2,5-diketo-D-gluconic acid (2,5-DKG) reductase from Corynebacterium sp. (278 aa), fasta scores; opt: 938, z-score: 1372.7, E(): 0, (52.9% identity in 276 aa overlap). Contains PS00063 Aldo /keto reductase family putative active site signature, PS00062 Aldo /keto reductase family signature 2 and Pfam match to entry PF00248 aldo_ket_red, Aldo /keto reductase family, score 464.50, E-value 3.6e-136. 4 2 2 16 [Reporter]SCO7302 (6P19)_PCR/3565 [Gene]SCO7302/3245 NC_003888 SC5F8.12c, hypothetical protein, len: 210 aa; similar to TR:AAK45279 (EMBL:AE006986) Mycobacterium tuberculosis CDC1551 hypothetical protein MT1029, 205 aa; fasta scores: opt: 784 Z-score: 926.8 E(): 5.5e-44; 56.796% identity in 206 aa overlap 4 2 2 15 [Reporter]SCO4413 (7L19)_PCR/3564 [Gene]SCO4413/3244 NC_003888 SC6F11.11c, probable araC family transcriptional regulatory protein, len: 344 aa; highly similar to TR:Q9S166 (EMBL:AB023785) Streptomyces griseus AdpA, 405 aa; fasta scores: opt: 881 z-score: 1024.1 E(): 0; 46.9% identity in 322 aa overlap and to TR:CAB70646 (EMBL:AL137242) Streptomyces coelicolor putative transcriptional regulator SC8F4.20c, 337 aa; fasta scores: opt: 1237 z-score: 1238.7 E(): 0; 60.3% identity in 325 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family and a possible helix-turn-helix motif at residues 249..270 (+2.55 SD) 4 2 2 14 [Reporter]SCO6876 (8H19)_PCR/3563 [Gene]SCO6876/3243 NC_003888 SC7F9.28c, unknown, len: 168 aa. 3 1 8 9 [Reporter]SCO1521 (13K20)_PCR/1170 [Gene]SCO1521/1069 NC_003888 SCL2.11c, hypothetical protein, len: 250 aa; similar to various hypothetical proteins, e.g. TR:YQ03_MYCTU (EMBL:Z98045) Mycobacterium tuberculosis hypothetical 26.8 kD protein RV2603c, 251 aa; fasta scores: opt: 1268 z-score: 1388.1 E(): 0; 76.7% identity in 249 aa overlap. Contains Pfam match to entry PF01709 DUF28, Domain of unknown function 4 2 2 13 [Reporter]SCO7152 (9D19)_PCR/3562 [Gene]SCO7152/3242 NC_003888 SC9A4.14, unknown, len: 274 aa. High content in alanine, arginine, glutamate and leucine amino acid residues 3 1 8 8 [Reporter]SCO1530 (14G20)_PCR/1169 [Gene]SCO1530/1068 NC_003888 SCL2.20c, hypothetical HIT family protein, len: 186 aa; similar to TR:O06201 (EMBL:Z95387) Mycobacterium tuberculosis hypothetical 21.8 kD protein MTCY01A10.20A, 195 aa; fasta scores: opt: 638 z-score: 762.0 E(): 0; 52.8% identity in 176 aa overlap and to TR:CAB48988 (EMBL:AJ248283) Pyrococcus abyssi HIT family protein, 185 aa; fasta scores: opt: 498 z-score: 597.6 E(): 6.8e-26; 45.1% identity in 164 aa overlap. similar also to TR:Q9F7Z0 (EMBL:AF265558) Mycobacterium smegmatis hypothetical protein 205 aa; fasta scores: opt: 653 Z-score: 762.7 E(): 7.5e-35; 52.432% identity in 185 aa overlap. Contains Pfam match to entry PF01230 HIT, HIT family 4 2 2 12 [Reporter]SCO2341 (10P15)_PCR/3561 [Gene]SCO2341/3241 NC_003888 SCC53.32c, unknown, len: 204aa; contains Pro/Arg/Thr-rich region residues 10-80. 3 1 8 7 [Reporter]SCO1816 (15C20)_PCR/1168 [Gene]SCO1816/1067 NC_003888 SCI8.01, unknown, partial CDS, len: > 104 aa. Similar to many proteins of undefined function e.g. Mycobacterium tuberculosis TR:P71897 (EMBL; Z79702) hypothetical 54.6 KD protein (505 aa), fasta scores opt: 596 z-score: 748.3 E(): 0 79.6% identity in 103 aa overlap. Overlaps and extends into CDS SCI28.10 on the adjoining cosmid,SCI28.10, partial CDS, unknown, len: >435aa; similar to many of undefined function egs. TR:P71897 (EMBL:Z79702) hypothetical protein from Mycobacterium tuberculosis (505 aa) fasta scores; opt: 1767, z-score: 2036.8, E(): 0, (63.3% identity in 431 aa overlap) and SW:TLDD_ECOLI TldD protein from Escherichia coli (481 aa) fasta scores; opt: 327, z-score: 379.0, E(): 8.9e-14, (28.0% identity in 415 aa overlap). 4 2 2 11 [Reporter]SCO4508 (11L15)_PCR/3560 [Gene]SCO4508/3240 NC_003888 SCD35.15, possible cell division-related protein, len: 1525 aa; similar to TR:P71068 (EMBL:Z99120) Bacillus subtilis YukA protein, 1207 aa; fasta scores: opt: 682 z-score: 717.6 E(): 1.5e-32; 23.7% identity in 1288 aa overlap and C-terminal region similar to TR:O25722 (EMBL:AE000615) Helicobacter pylori (Campylobacter pylori) cell division protein (FtsK), 858 aa; fasta scores: opt: 347 z-score: 364.2 E(): 7.4e-13; 33.1% identity in 245 aa overlap. Contains two Pfam matches to entry PF01580 FtsK_SpoIIIE, FtsK/SpoIIIE family and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Contains possible hydrophobic membrane spanning region 3 1 8 6 [Reporter]SCO0669 (16O16)_PCR/1167 [Gene]SCO0669/1066 NC_003888 SCF91.29, possible TetR-family transcriptional regulator, len: 205 aa. Weakly similar to many other regulators e.g. Streptomyces hygroscopicus TR:Q54288 (EMBL:X86780) regulator of antibiotic transport complexes (204 aa), fasta scores opt: 148 z-score: 192.6 E(): 0.0024 29.6% identity in 115 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 37..58 (+5.86 SD). 3 1 8 5 [Reporter]SCO0495 (17K16)_PCR/1166 [Gene]SCO0495/1065 NC_003888 SCF34.14c, probable iron-siderophore ABC-transporter ATP-binding protein, len: 287 aa; similar to SW:FEPC_ECOLI (EMBL:X57471), FepC, Escherichia coli ferric enterobactin transport ATP-binding protein (271 aa), fasta scores; opt: 946 z-score: 1003.1 E(): 0, 52.3% identity in 262 aa overlap and to many other iron-siderophore ATP-binding proteins. Similar to many others from S. coelicolor e.g. SCI51.25c (EMBL:AL109848) S.coelicolor probable iron-siderophore uptake system ATP-binding component (301 aa) (58.9% identity in 258 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 8 4 [Reporter]SCO1461 (18G16)_PCR/1165 [Gene]SCO1461/1064 NC_003888 SCL6.18c, guaB2, probable inosine monophosphate dehydrogenase, len: 483 aa; similar to SW:IMDH_BACSU (EMBL:X55669) Bacillus subtilis inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) GuaB, 513 aa; fasta scores: opt: 970 z-score: 1018.4 E(): 0; 37.4% identity in 471 aa overlap. Contains Pfam match to entry PF01574 IMPDH_N, IMP dehydrogenase / GMP reductase N terminus, two matches to entry PF00571 CBS, CBS domain and to entry PF00478 IMPDH_C, IMP dehydrogenase / GMP reductase C terminus 3 1 8 3 [Reporter]SCO4022 (19C16)_PCR/1164 [Gene]SCO4022/1063 NC_003888 2SC10A7.26, probable glycosyl transferase, len: 449 aa; similar to N-terminal region of TR:AAF72730 (EMBL:AF265258) Rhodococcus sp. S9 dolichyl-phosphate beta-glucosyltransferase, 342 aa; fasta scores: opt: 1385 z-score: 1585.5 E(): 0; 66.0% identity in 326 aa overlap. Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases and possible hydrophobic membrane spanning regions at C-terminal domain. Also contains a 5x degenerate repeat unit: PDPH(L/R) 3 1 8 2 [Reporter]SCO5060 (20K12)_PCR/1163 [Gene]SCO5060/1062 NC_003888 SCBAC20F6.03c, possible integral membrane protein, len: 200 aa. Contains possible hydrophobic membrane spanning regions and high G+C content in N-terminal region 3 1 7 22 [Reporter]SCO1228 (1K8)_PCR/1161 [Gene]SCO1228/1061 NC_003888 2SCG1.03, probable acyltransferase, len: 240 aa; similar to TR:CAB76086 (EMBL:AL157956) Streptomyces coelicolor putative acyltransferase SCL24.02, 223 aa; fasta scores: opt: 786 z-score: 912.9 E(): 0; 51.1% identity in 219 aa overlap and to TR:CAB51970 (EMBL:AL109661) Streptomyces coelicolor hypothetical protein SC6E10.16c, 262 aa; fasta scores: opt: 912 z-score: 945.5 E(): 0; 59.6% identity in 240 aa overlap. Contains Pfam match to entry PF01553 Acyltransferase, Acyltransferase 3 1 7 21 [Reporter]SCO4425 (2G8)_PCR/1160 [Gene]SCO4425/1060 NC_003888 SCD6.03c, afsR2, regulator of antibiotic production, len: 63 aa; identical to previously sequenced TR:Q57058 (EMBL:D45382) Streptomyces coelicolor AfsR2, 63 aa 4 3 19 11 [Reporter]SCO3958 (12P6)_PCR/5950 [Gene]SCO3958/5419 NC_003888 SCD78.25, ABC transporter ATP-binding protein, len: 303 aa; similar to TR:Q45404 (EMBL:U09819) SpaFABC transporter ATP-binding protein (247 aa) from Bacillus subtilis, fasta scores;opt: 524, z-score: 615.6, E(): 4.8e-27, (39.3% identity in 234 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporters, score 161.50, E-value 1.4e-44 and Prosite matches to PS00017 ATP /GTP-binding= PS00211 ABC transporters family signature 4 3 19 10 [Reporter]SCO3272 (13L6)_PCR/5949 [Gene]SCO3272/5418 NC_003888 SCE39.22c, possible DNA-binding protein, len: 122aa; some similarity to TR:O64356 (EMBL:AF064539) Gp48 protein from bacteriophage N15 (95 aa) fasta scores; opt: 114, z-score: 155.2, E(): 0.25, (36.6% identity in 71 aa overlap). Contains probable helix-turn-helix (+6.85 SD) 82-103aa. 4 3 19 9 [Reporter]SCO0954 (14H6)_PCR/5948 [Gene]SCO0954/5417 NC_003888 SCM11.09c, possible acetyltransferase, len: 160 aa; similar to various hypothetical proteins, e.g. TR:Q51670 (EMBL:U34346) Paracoccus denitrificans hypothetical 18.8 kD protein, 173 aa; fasta scores: opt: 427 z-score: 515.7 E(): 2.4e-21; 45.3% identity in 161 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 4 3 19 8 [Reporter]SCO3910 (15D6)_PCR/5947 [Gene]SCO3910/5416 NC_003888 SCH24.32c, putative membrane protein, len: 448 aa; similar to many e.g. SW:DINF_ECOLI (EMBL:L02362), DinF, Escherichia coli DNA-damage-inducible protein (459 aa), fasta scores; opt: 571 z-score: 655.4 E(): 3.4e-29, 30.0% identity in 437 aa overlap. There is an alternative start codon at the third amino acid. Contains possible hydrophobic membrane spanning regions 4 3 19 7 [Reporter]SCO0693 (16P2)_PCR/5946 [Gene]SCO0693/5415 NC_003888 SCF42.03, possible membrane protein, len: 115 aa. Contains a possible N-terminal signal sequence and a possible membrane spanning hydrophobic domain. Rich in Ala and Leu. 4 3 19 6 [Reporter]SCO3151 (17L2)_PCR/5945 [Gene]SCO3151/5414 NC_003888 SCE87.02c, unknown, len: 296 aa. Highly similar to many hypothetical proteins including: Mycobacterium tuberculosis TR:O08343 (EMBL: Z94752) hypothetical protein YcfH (264 aa), fasta scores opt: 576 z-score: 667.6 E(): 8.2e-30 42.7% identity in 279 aa overlap and Escherichia coli SW:YCFH_ECOLI (EMBL:AE000210) hypothetical 29.8 KD protein (265 aa), fasta scores opt: 477 z-score: 554.2 E(): 1.7e-23 37.0% identity in 276 aa overlap. Contains a Pfam match to entry PF01026 UPF0006, Uncharacterized protein family 4 3 19 5 [Reporter]SCO3224 (18H2)_PCR/5944 [Gene]SCO3224/5413 NC_003888 SCE8.17c, probable ABC transporter ATP-binding protein, len: 317aa; similar to many egs. TR:O86629 (EMBL:AL031155) ABC transporter ATP-binding protein from Streptomyces coelicolor (312 aa) fasta scores; opt: 1080, z-score: 1174.5, E(): 0, (57.4% identity in 303 aa overlap) and SW:BCRA_BACLI bacitracin-resistance ABC transporter from Bacillus licheniformis (306 aa) fasta scores; opt: 567, z-score: 621.0, E(): 2.8e-27, (42.4% identity in 217 aa overlap). N-terminal region previously sequenced therefore identical to TR:P72389 (EMBL:U51332) partial, designated D7 (103 aa) fasta scores; opt: 666, z-score: 734.8, E(): 0, (99.0% identity in 103 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, score 151.20, E-value 1.9e-41 and PS00017 ATP/GTP-binding site motif A (P-loop). 4 3 19 4 [Reporter]SCO1367 (19D2)_PCR/5943 [Gene]SCO1367/5412 NC_003888 SC10A9.09c, probable ABC transport system ATP-binding protein, len: 251 aa; similar to SW:LOLD_ECOLI (EMBL:AE000212) Escherichia coli lipoprotein releasing system ATP-binding protein LolD, 233 aa; fasta scores: opt: 530 z-score: 576.0 E(): 1.2e-24; 40.8% identity in 218 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 4 2 2 10 [Reporter]SCO4255 (12H15)_PCR/3559 [Gene]SCO4255/3239 NC_003888 SCD8A.28, unknown, len: 242 aa 4 3 19 2 [Reporter]SCO5992 (20H22)_PCR/5941 [Gene]SCO5992/5411 NC_003888 SCBAC16H6.27, possible integral membrane protein, len: 164aa: no significant database matches. Contains possible membrane-spanning hydrophobic regions. 4 2 2 9 [Reporter]SCO3320 (13D15)_PCR/3558 [Gene]SCO3320/3238 NC_003888 SCE68.18c, hypothetical protein, len: 258 aa; similar to P25 (EMBL:AF061257) Thermus aquaticus AT-rich DNA-binding protein (211 aa), fasta scores; opt: 529 z-score: 604.8 E(): 2.3e-26, 44.9% identity in 198 aa overlap. Also similar to hypothetical proteins from Bacillus subtilis TR:O05521 (EMBL:D88802), YdiH (215 aa) (40.9% identity in 198 aa overlap) and Bacillus halodurans (EMBL:AB013375), YdiH (211 aa) (42.9% identity in 198 aa overlap) 4 3 18 22 [Reporter]SCO1118 (1H18)_PCR/5939 [Gene]SCO1118/5410 NC_003888 2SCG38.11c, possible integral membrane protein, len: 496 aa; low similarity to TR:AAG03843 (EMBL:AE004483) Pseudomonas aeruginosa conserved hypothetical protein PA0454, 733 aa; fasta scores: opt: 224 z-score: 245.0 E(): 3.3e-06; 28.0% identity in 300 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 2 8 [Reporter]SCO4944 (14P11)_PCR/3557 [Gene]SCO4944/3237 NC_003888 2SCK31.04c, possible DNA-binding protein, len: 314 aa; similar to TR:Q9RI67 (EMBL:AL109949) Streptomyces coelicolor putative DNA-binding protein SCJ11.39c, 279 aa; fasta scores: opt: 544 z-score: 640.7 E(): 3.8e-28; 40.2% identity in 249 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and possible helix-turn-helix motif at residues 64..85 (+4.67 SD) 4 2 2 7 [Reporter]SCO3664 (15L11)_PCR/3556 [Gene]SCO3664/3236 NC_003888 SCH44.04, possible regulatory protein, len: 336 aa; similar to TR:Q56084 (EMBL:D63904) Streptomyces thermoviolaceus ESA36 (erythropoiesis-stimulating activity), 344 aa; fasta scores: opt: 339 z-score: 402.5 E(): 5.9e-15; 29.7% identity in 286 aa overlap and to Streptomyces coelicolor SCH44.05 aa; fasta scores: opt: 973 z-score: 1035.9 E(): 0; 48.0% identity in 325 aa overlap and to SCH44.06c, 394 aa; fasta scores: opt: 952 z-score: 1012.5 E(): 0; 48.1% identity in 320 aa overlap. Contains possible helix-turn-helix motif at residues 278..299 (+3.88 SD) 4 2 2 6 [Reporter]SCO0155 (16H11)_PCR/3555 [Gene]SCO0155/3235 NC_003888 SCJ1.04, possible tetR family transcriptional regulatory protein, len: 204 aa, similarity to several E.coli proteins including uid operon repressor (gus operon repressor) SW:UIDR_ECOLI (EMBL:M14641)( 196 aa), fasta scores opt: 170 z-score: 212.8 E(): 0.00017 25.5%identity in 184 aa overlap and the hypothetical transcriptional regulator SW:YDHM_ECOLI (EMBL: AE000260)(199 aa), fasta scores opt: 176 z-score: 219.8 E(): 7e-05 27.6% identity in 185 aa overlap. In addition to Lactococcus lactis (subsp. lactis) hypothetical protein SW:YPB3_LACLA (EMBL: X60456) (196 aa), fasta scores opt: 245 z-score: 301.6 E(): 1.9e-09 31.9% identity in 188 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family, 4 2 2 5 [Reporter]SCO3344 (17D11)_PCR/3554 [Gene]SCO3344/3234 NC_003888 SCE7.11, probable serine/threonine protein kinase, len: 720 aa; similar to many from other Streptomyces spp. eg. SW:AFSK_STRCO serine/threonine protein kinase from Streptomyces coelicolor (799 aa) fasta scores; opt: 896, z-score: 827.4, E(): 0, (33.1% identity in 771 aa overlap). Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and Prosite matches to PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature. Also contains five Pfam matches to entry PF01011 Bacterial_PQQ, PQQ enzyme repeat. 4 2 2 4 [Reporter]SCO0848 (18P7)_PCR/3553 [Gene]SCO0848/3233 NC_003888 SCM2.01c, possible oxidoreductase, len: 333 aa. Similar to many including several from eukaryotes e.g. Mus musculus (Mouse) SW:QOR_MOUSE(EMBL:S70056) quinone oxidoreductase (EC 1.6.5.5) (331 aa), fasta scores opt: 499 z-score: 508.7 E(): 5.9e-21 30.5% identity in 331 aa overlap. Also similar to several from Prokaryotes e.g. Streptomyces coelicolor TR:Q53927(EMBL:X62373) hydroxyacyl-CoA dehydrogenase involved in actinorhodin biosynthesis (329 aa), fasta scores opt: 481 z-score: 490.8 E(): 5.8e-20 35.8% identity in 338 aa overlap. Contains a Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases. 3 1 7 20 [Reporter]SCO5699 (3C8)_PCR/1159 [Gene]SCO5699/1059 NC_003888 SC5H4.23, proS, prolyl tRNA synthetase, len: 567 aa; highly similar to SW:SYP_ECOLI (EMBL:X55518) Escherichia coli prolyl-tRNA synthetase (EC 6.1.1.15) ProS, 572 aa; fasta scores: opt: 1403 z-score: 1520.4 E(): 0; 41.8% identity in 574 aa overlap. Contains Pfam match to entry PF00587 tRNA-synt_2b, tRNA synthetase class II (G, H, P, S and T) and match to Prosite entry PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1 4 2 2 3 [Reporter]SCO1977 (19L7)_PCR/3552 [Gene]SCO1977/3232 NC_003888 SC3C9.12c, possible glutamate synthase small subunit, len: 496 aa; similar to C-terminal part of SW:GLSN_MEDSA (EMBL:L01660) Medicago sativa glutamate synthase [NADH], chloroplast precursor (EC 1.4.1.14) (NADH-GOGAT) GlsN, 2194 aa; fasta scores: opt: 1473 Z-score: 1543.9 bits: 297.3 E(): 2.2e-78; 47.551% identity in 490 aa overlap and to TR:Q9S2Z0 (EMBL:AL109849) Streptomyces coelicolor putative glutamate synthase small subunit SC3A3.03c, 487 aa; fasta scores: opt: 2145 Z-score: 2259.1 bits: 427.5 E(): 3.2e-118; 66.398% identity in 497 aa overlap. Contains Pfam match to entry PF00070 pyr_redox, Pyridine nucleotide-disulphide oxidoreductase 3 1 7 19 [Reporter]SCO6612 (4O4)_PCR/1158 [Gene]SCO6612/1058 NC_003888 SC1F2.09c, probable hydrolase, len: 309 aa; similar to many e.g. XYLF_PSEPU 2-hydroxymuconic semialdehyde hydrolase (281 aa), fasta scores; opt: 236 z-score: 348.2 E(): 3.8e-12, 29.0% identity in 283 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha /beta hydrolase fold, score 52.80, E-value 7.5e-12 4 2 2 2 [Reporter]SCO1876 (20D7)_PCR/3551 [Gene]SCO1876/3231 NC_003888 SCI39.23, possible RNA polymerase sigma factor, len: 190 aa; similar to SW:RPOE_STRCO (EMBL:L29636) Streptomyces coelicolor RNA polymerase sigma-E factor SigE, 176 aa; fasta scores: opt: 210 Z-score: 262.1 bits: 55.1 E(): 5.2e-07; 34.177% identity in 158 aa overlap. Contains possible helix-turn-helix motif at residues 132..153 (+3.59 SD) 3 1 7 18 [Reporter]SCO5896 (5K4)_PCR/1157 [Gene]SCO5896/1057 NC_003888 SC10A5.01, redH, probable phosphoenolpyruvate-utilizing enzyme, partial CDS, len: >95 aa; overlaps and extends ORF from neighbouring cosmid SC3F7.16; similar to many eg. PPSA_STAMA P46893 probable phosphoenolpyruvate synthase (834 aa), fasta scores; opt: 215 z-score: 351.4 E(): 2.3e-12, 47.8% identity in 69 aa overlap,SC3F7.16, probable phosphoenolpyruvate-utilizing enzyme, partial CDS, len: >872 aa; similar to many eg. PPSA_STAMA P46893 probable phosphoenolpyruvate synthase from Staphylothermus marinus (834 aa), fasta scores; opt: 392 z-score: 614.9 E(): 4.7e-27, 33.0% identity in 309 aa overlap. Contains Pfam match to entry PEP-utilizers PF00391, PEP-utilizing enzymes, score 23.96 4 2 1 22 [Reporter]SCO5196 (1D3)_PCR/3549 [Gene]SCO5196/3230 NC_003888 2SC3B6.20, conserved hypothetical protein, len: 226 aa; similar to TR:CAB93731 (EMBL:AL357613) Streptomyces coelicolor hypothetical 23.8 kD protein SC5F8.03c, 225 aa; fasta scores: opt: 439 z-score: 523.3 E(): 1.1e-21; 37.8% identity in 222 aa overlap 3 1 7 17 [Reporter]SCO6648 (6G4)_PCR/1156 [Gene]SCO6648/1056 NC_003888 SC4G2.22, unknown, len: 112 aa 3 1 7 15 [Reporter]SCO2138 (7O24)_PCR/1154 [Gene]SCO2138/1055 NC_003888 SC6G10.11c, len: 79aa; 3 1 7 14 [Reporter]SCO6588 (8K24)_PCR/1153 [Gene]SCO6588/1054 NC_003888 SC8A6.09c, unknown, len: 443 aa; some similarity to M. tuberculosis hypothetical protein Y029_MYCTU MTCY373.09 C (521 aa), fasta scores; opt: 371 z-score: 502.3 E(): 9.7e -21, 28.3% identity in 446 aa overlap 3 1 7 12 [Reporter]SCO2311 (10C24)_PCR/1151 [Gene]SCO2311/1053 NC_003888 SCC53.02, unknown, len: 542aa; contains possible stem loop in the 3' end of the CDS. 3 1 7 11 [Reporter]SCO4664 (11O20)_PCR/1150 [Gene]SCO4664/1052 NC_003888 SCD40A.10c, possible membrane protein, len: 611 aa; similar to C-terminal region of TR:CAB71211 (EMBL:AL138538) Streptomyces coelicolor putative membrane protein SC6D10.16, 907 aa; fasta scores: opt: 262 z-score: 270.5 E(): 1.2e-07; 29.0% identity in 630 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 7 10 [Reporter]SCO2936 (12K20)_PCR/1149 [Gene]SCO2936/1051 NC_003888 SCE19A.36c, conserved hypothetical protein, len: 598 aa; unknown function, similar to mycobacterial hypothetical proteins SW:Y065_MYCTU (EMBL:Z74697) Mycobacterium tuberculosis hypothetical protein (626 aa), fasta scores; opt: 1921 z-score: 2072.6 E(): 0, 56.1% identity in 567 aa overlap and TR:O33011 (EMBL:Z97369) Mycobacterium leprae hypothetical protein (596 aa) (55.7% identity in 566 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 7 9 [Reporter]SCO0935 (13G20)_PCR/1148 [Gene]SCO0935/1050 NC_003888 SCM10.23, possible secreted protein, len: 221 aa. Contains N-terminal region signal peptide sequence 4 3 18 21 [Reporter]SCO5364 (2D18)_PCR/5938 [Gene]SCO5364/5409 NC_003888 2SC6G5.08, glyA3, serine hydroxymethyltransferase (EC 2.1.2.1), len: 418 aa; strongly similar to many e.g. SW:P00477 (GLYA_ECOLI) serine hydroxymethyltransferase from Escherichia coli (417 aa) fasta scores; opt: 1164, z-score: 1265.0, E(): 0, 47.9% identity in 407 aa overlap. Contains Pfam match to entry PF00464 SHMT, Serine hydroxymethyltransferase. Also similar to two others from Streptomyces coelicolor; SC2A11.04c (SW:O86565 (GLYA_STRCO)) and SC5G8.05 (TR:CAB89056 (EMBL:AL353872)). 4 3 18 20 [Reporter]SCO6811 (3P14)_PCR/5937 [Gene]SCO6811/5408 NC_003888 SC1A2.20c, possible secreted protein, len: 464 aa. The N-terminus is highly similar to Streptomyces coelicolor TR:Q9X8X7 (EMBL:AL078610) putative secreted protein, SCH35.29 (162 aa), fasta scores opt: 753 z-score: 813.4 E(): 0 71.2% identity in 146 aa overlap. Contains a possible N-terminal signal sequence. 4 3 18 19 [Reporter]SCO7209 (4L14)_PCR/5936 [Gene]SCO7209/5407 NC_003888 SC2H12.08c, doubtful CDS, len: 46 aa. 4 3 18 18 [Reporter]SCO2382 (5H14)_PCR/5935 [Gene]SCO2382/5406 NC_003888 SC4A7.10, hypothetical protein, len: 65 aa; similar to various hypothetical proteins, e.g. TR:Q9Z544 (EMBL:AL035212) Streptomyces coelicolor hypothetical 8.2 kD protein, 77 aa; fasta scores: opt: 186 z-score: 270.2 E(): 1.1e-07; 60.0% identity in 55 aa overlap, to S. coelicolor SC2H4.17c, 81 aa; fasta scores: opt: 165 z-score: 212.0 E(): 2e-06; 50.0% identity in 60 aa overlap and to S. coelicolor SC4G2.04, 67 aa; fasta scores: opt: 149 z-score: 195.0 E(): 1.8e-05; 51.0% identity in 51 aa overlap 4 3 18 17 [Reporter]SCO7762 (6D14)_PCR/5934 [Gene]SCO7762/5405 NC_003888 SC5E9.10, unknown, doubtful CDS, len: 320 aa; appears to lie within a region of degraded DNA. 4 3 18 16 [Reporter]SCO6790 (7P10)_PCR/5933 [Gene]SCO6790/5404 NC_003888 SC6A5.39, probable long chain fatty acid coA ligase, len: aa; similar to many eg. TR:E1359128 putative long chain fatty acid coA ligase from Streptomyces coelicolor (608 aa) fasta scores; opt: 943, z-score: 1037.6, E(): 0, (34.8% identity in 621 aa overlap) and SW:LCFX_RAT long chain fatty acid coA ligase from Rattus norvegicus (Rat) (697 aa) fasta scores; opt: 532, z-score: 584.9, E(): 2.8e-25, (28.0% identity in 626 aa overlap). Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and Prosite match to PS00455 Putative AMP-binding domain signature. 4 3 18 15 [Reporter]SCO6008 (8L10)_PCR/5932 [Gene]SCO6008/5403 NC_003888 SC7B7.05, probable transcriptional repressor protein, len 403 aa; similar to many eg. XYLR_ANATH Q44406 xylose repressor from Anaerocellum thermophilum (399 aa), fasta scores; opt: 548 z-score: 767.3 E(): 0, 33.1% identity in 393 aa overlap. Contains PS01125 ROK family signature and helix-turn-helix motif at aa positions 29-50 (Score 2016, +6.05 SD) 4 3 18 14 [Reporter]SCO5509 (9H10)_PCR/5931 [Gene]SCO5509/5402 NC_003888 SC8D9.21c, probable oxidoreductase, len: 447aa; similar to many both prokaryotic and eukaryotic egs. SW:A115_TOBAC auxin induced protein (probable oxidoreductase) from Nicotiana tabacum (common tobacco) (307 aa) fasta scores; opt: 391, z-score: 456.6, E(): 4e-18, (36.7% identity in 289 aa overlap) and TR:O07152 (EMBL:Z96801) putative oxidoreductase from Mycobacterium leprae (306 aa) fasta scores; opt: 336, z-score: 393.5, E(): 1.3e-14, (30.8% identity in 295 aa overlap). Contains Pfam match to entry PF00248 aldo_ket_red, Aldo/keto reductase family, score 33.70, E-value 4.6e-09. 4 2 1 21 [Reporter]SCO4924 (1P23)_PCR/3548 [Gene]SCO4924/3229 NC_003888 SCK13.16c, possible membrane protein, len: 42 aa. Contains possible hydrophobic membrane spanning region 4 3 18 13 [Reporter]SCO7370 (10D10)_PCR/5930 [Gene]SCO7370/5401 NC_003888 SC9H11.24, NAD-dependent dehydratase, len: 346 aa. Similar to many members of the sugar epimerase/dehydratase family including: Streptomyces griseus SW:STRE_STRGR(EMBL:X62567) DTDP-glucose 4,6-dehydratase (EC 4.2.1.46) (328 aa), fasta scores opt: 363 z-score: 378.8 E(): 1.2e-13 31.8% identity in 336 aa overlap and Streptomyces coelicolor TR:Q9ZBN0 (EMBL:AL034492) putative nucleotide-sugar dehydratase, SC6C5.15 (330 aa), fasta scores opt: 977 z-score: 1003.9 E(): 0 52.6% identity in 321 aa overlap. Contains a Pfam match to entry PF01370 Epimerase, NAD dependent epimerase/dehydratase family. 4 2 1 20 [Reporter]SCO4599 (2L23)_PCR/3547 [Gene]SCO4599/3228 NC_003888 SCD20.17, nuoA2, NADH dehydrogenase subunit, len: 146 aa; similar to SW:NUOA_RHOCA (EMBL:AF029365) Rhodobacter capsulatus NADH dehydrogenase I chain A (EC 1.6.5.3) NuoA, 126 aa; fasta scores: opt: 260 z-score: 336.3 E(): 2.9e-11; 36.2% identity in 130 aa overlap and to TR:Q9XAQ4 (EMBL:AL078618) Streptomyces coelicolor NuoA, NADH dehydrogenase subunit SCD16A.21c, 119 aa; fasta scores: opt: 231 z-score: 278.0 E(): 7.5e-10; 31.4% identity in 118 aa overlap. Contains Pfam match to entry PF00507 oxidored_q4, NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 and possible hydrophobic membrane spanning regions 4 3 18 12 [Reporter]SCO4384 (11P6)_PCR/5929 [Gene]SCO4384/5400 NC_003888 SCD10.16, possible enoyl CoA hydratase, len: 247 aa; similar to TR:Q9X7Q4 (EMBL:AL049587) Streptomyces coelicolor putative enoyl CoA hydratase SC5F2A.31c, 257 aa; fasta scores: opt: 364 z-score: 425.2 E(): 3.3e-16; 36.1% identity in 252 aa overlap and to SW:ECHH_RHIME (EMBL:L39265) Rhizobium meliloti probable enoyl-CoA hydratase (EC 4.2.1.17) FadB1, 257 aa; fasta scores: opt: 334 z-score: 390.9 E(): 2.7e-14; 34.0% identity in 247 aa overla. Contains Pfam match to entry PF00378 ECH, Enoyl-CoA hydratase/isomerase family 4 2 1 19 [Reporter]SCO6326 (3H23)_PCR/3546 [Gene]SCO6326/3227 NC_003888 SC10H5.02c, probable integral membrane protein, len: 151 aa; similar to S. coelicolor hypothetical protein TR:O54194 (EMBL:AL021411) SC7H1.35 (155 aa), fasta scores; opt: 431 z-score: 749.8 E(): 0, 53.5% identity in 114 aa overlap 4 2 1 18 [Reporter]SCO6194 (4D23)_PCR/3545 [Gene]SCO6194/3226 NC_003888 SC2G5.15c, possible response regulator, len:279aa; similar to many eg. TR:P72471 (EMBL:U63847) response regulator homolog from Streptomyces lividans (225aa) fasta scores; opt: 184, z-score: 203.6, E():0.00047, (32.7% identity in 208 aa overlap). Also C-terminal region similar to TR:P72402 (EMBL:Y07902) transcriptional regulator, RedZ (217 aa) fasta scores; opt:179, z-score:198.6, E():0.00089, (47.6 % identity in 63 aa overlap). Contains Pfam match to en try PF00196 GerE, Bacterial regulatory proteins, luxR family, score 58.30, E-value 1.7e-13. Contains possible helix-turn-helix at 234-255aa Score 1690 (+4.94 SD). 4 2 1 17 [Reporter]SCO7038 (5P19)_PCR/3544 [Gene]SCO7038/3225 NC_003888 SC4G1.04c, possible integral membrane protein, len: 760 aa. Contains possible hydrophobic membrane spanning regions and cleavable N-terminal signal peptide sequence 4 2 1 16 [Reporter]SCO7298 (6L19)_PCR/3543 [Gene]SCO7298/3224 NC_003888 SC5F8.08c, possible thioredoxin reductase, len: 558 aa. The C-terminus of this protein is highly similar to many thioredoxin reductases including: Streptomyces clavuligerus SW:TRXB_STRCL(EMBL:Z21946) thioredoxin reductase (EC 1.6.4.5) (321 aa), fasta scores opt: 494 z-score: 519.8 E(): 1.7e-21 32.8% identity in 332 aa overlap. The N-terminus is similar to the receiver domains of many response regulators e.g. Alcaligenes eutrophus SW:HOXA_ALCEU(EMBL:M64593) hydrogenase transcriptional regulatory protein, HoxA (482 aa) (32% identity over a 117 aa overlap). Contains Pfam matches to entries PF00070 pyr_redox, Pyridine nucleotide-disulphide oxidoreductase and PF00072 response_reg, Response regulator receiver domain. 4 2 1 15 [Reporter]SCO7407 (7H19)_PCR/3542 [Gene]SCO7407/3223 NC_003888 SC6D11.03c, probable beta-galactosidase, len: 681 aa. Similar to many beta-galactosidases e.g. Bacillus stearothermophilus SW:BGAL_BACST(EMBL:M13466) beta-galactosidase I (EC 3.2.1.23), BgaB, (672 aa), fasta scores opt: 1816 z-score: 2079.7 E(): 0 43.2% identity in 666 aa overlap. 3 1 7 8 [Reporter]SCO3860 (14C20)_PCR/1147 [Gene]SCO3860/1049 NC_003888 SCH69.30, possible serine/threonine protein kinase, len: 576 aa; similar to many e.g. TR:CAA09628 (EMBL:AJ011500) putative serine/threonine protein kinase from the granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22 (433 aa) fasta scores; opt: 1791, z-score: 976.0, E(): 0, (65.2% identity in 428 aa overlap) and SW:PKAA_STRCO PkaA, serine/threonine protein kinase (543 aa) fasta scores; opt: 576, z-score: 329.1, E(): 5.2e-11, (29.6% identity in 564 aa overlap). Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain. Contains possible hydrophobic membrane spanning region 4 2 1 14 [Reporter]SCO6872 (8D19)_PCR/3541 [Gene]SCO6872/3222 NC_003888 SC7F9.24c, unknown, len: 303 aa. Similar in parts to Streptomyces coelicolor TR:Q9X875(EMBL:AL049661) hypothetical 21.6 KD protein, SCE134.20 (203 aa), fasta scores opt: 358 z-score: 349.8 E(): 4.5e-12 43.8% identity in 146 aa overlap. 3 1 7 7 [Reporter]SCO3722 (15O16)_PCR/1146 [Gene]SCO3722/1048 NC_003888 SCH35.02, possible transcriptional regulator, len: 117aa; similar to many eg. TR:O53626 (EMBL:AL021428) putative transcriptional regulator from Mycobacterium tuberculosis (114 aa) fasta scores; opt: 404, z-score: 526.2, E(): 5.6e-22, (58.2% identity in 110 aa overlap) and SW:NOLR_RHIME NolR negative transacting factor controlling the nod regulon in Rhizobium meliloti (122 aa) fasta scores; opt: 175, z-score: 237.2, E(): 7e-06, (35.2% identity in 88 aa overlap). Contains Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family. 4 2 1 13 [Reporter]SCO6119 (9P15)_PCR/3540 [Gene]SCO6119/3221 NC_003888 SC9B2.06, possible AraC family transcriptional regulator, len: 227aa; similar to many eg. SW:YMCR_STRLA proposed transcriptional regulator from Streptomyces lavendulae (281 aa) fasta scores; opt: 860, z-score: 947.3, E(): 0, (60.2% identity in 231 aa overlap) and C-terminal similarity to SW:RHAS_ECOLI RhaS, L-rhamnose operon regulatory protein from Escherichia coli (278 aa) fasta scores; opt: 119, z-score: 140.2, E(): 1.6, (28.4% identity in 81 aa overlap). Contains PS00041 Bacterial regulatory proteins, araC family signature and Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. 3 1 7 6 [Reporter]SCO0809 (16K16)_PCR/1145 [Gene]SCO0809/1047 NC_003888 SCF43.20c. possible ABC transporter permease, len: 337 aa. Similar to many permeases e.g. Bacillus subtilis SW:RBSC_BACSU(EMBL:Z25798) ribose transport system permease protein RbsC (323 aa), fasta scores opt: 561 z-score: 603.2 E(): 3.3e-26 33.9% identity in 295 aa overlap. Also similar to the adjoining CDS, SCF43.21c, possible ABC transporter permease (346 aa), fasta scores opt: 545 z-score: 528.3 E(): 5e-24 33.4% identity in 335 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 2 1 12 [Reporter]SCO2337 (10L15)_PCR/3539 [Gene]SCO2337/3220 NC_003888 SCC53.28, possible acetyltransferase, len: 186 aa; regions similar to many of undefined function e.g. TR:CAB76276 (EMBL:AL158057) putative acetyltransferase from Streptomyces coelicolor (264 aa) fasta scores; opt: 131, z-score: 161.0, E(): 0.17, 37.5% identity in 112 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 3 1 7 5 [Reporter]SCO3363 (17G16)_PCR/1144 [Gene]SCO3363/1046 NC_003888 SCE94.14, possible acetyltransferase, len: 147aa; weakly similar to many eg. SW:PAT_STRHY phosphinothricin acetyltransferase from Streptomyces hygroscopicus (183 aa) fasta scores; opt: 116, z-score: 157.5, E(): 0.19, (27.9% identity in 136 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 3 1 7 4 [Reporter]SCO1551 (18C16)_PCR/1143 [Gene]SCO1551/1045 NC_003888 SCL11.07, possible eukaryotic-type protein kinase, len: 493 aa; similar to TR:Q9ZN80 (EMBL:AB021679) Streptomyces coelicolor PkaG, 592 aa; fasta scores: opt: 926 z-score: 659.3 E(): 2.6e-29; 41.0% identity in 417 aa overlap and N-terminal region similar to SW:AFSK_STRGR0 (EMBL:D45246) Streptomyces griseus serine/threonine protein kinase AfsK (EC 2.7.1.-) 807 aa; fasta scores: opt: 871 z-score: 619.6 E(): 4.3e-27; 39.3% identity in 435 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and matches to Prosite entries PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature. Contains possible hydrophobic membrane spanning region at C-terminal domain 3 1 7 3 [Reporter]SCO6088 (19O12)_PCR/1142 [Gene]SCO6088/1044 NC_003888 SCBAC1A6.12, possible solute-binding transport protein, len: 430 aa; similar to TR:Q9PAP7 (EMBL:AE004053) Xylella fastidiosa ABC transporter sugar-binding protein XF2448, 430 aa; fasta scores: opt: 351 z-score: 392.9 E(): 2.6e-14; 26.2% identity in 428 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and properly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 3 1 7 2 [Reporter]SCO6302 (20G12)_PCR/1141 [Gene]SCO6302/1043 NC_003888 SCIF3.04, unknown, len: 56 aa 3 1 6 22 [Reporter]SCO5256 (1G8)_PCR/1139 [Gene]SCO5256/1042 NC_003888 2SC7G11.18c, hypothetical protein, len: 209 aa; similar to TR:CAB70944 (EMBL:AL137778) Streptomyces coelicolor hypothetical 20.3 kDa protein SCL2.32, 186 aa; fasta scores: opt: 314 z-score: 396.2 E(): 1.3e-14; 36.6% identity in 183 aa overlap 3 1 6 21 [Reporter]SCO4723 (2C8)_PCR/1138 [Gene]SCO4723/1041 NC_003888 SCD31.48, adk, adenylate kinase (fragment), len: >157 aa; identical to previously sequenced SW:KAD_STRCO (EMBL:X83011) Streptomyces coelicolor adenylate kinase (EC 2.7.4.3) (ATP-AMP transphosphorylase) Adk, 217 aa. Contains Pfam match to entry PF00406 adenylatekinase, Adenylate kinase and matches to Prosite entry PS00113 Adenylate kinase signature,SC6G4.01, adk, adenylate kinase, partial CDS, len: > 94 aa; almost identical to KAD_STRCO adenylate kinase (EC 2 .7.4.3) (205 aa) and highly similar to many e.g. KAD_BORPE adenylate kinase (EC 2.7.4.3)(218 aa), fasta scores; opt: 2 53 z-score: 429.1 E(): 1.2e-16, 50.6% identity in 85 aa ove rlap. Contains Pfam match to entry PF00406 adenylatekinase, Adenylate kinases, score 136.80, E-value 3.2e-37 3 1 6 20 [Reporter]SCO2597 (3O4)_PCR/1137 [Gene]SCO2597/1040 NC_003888 SCC88.08c, rplU, 50S ribosomal protein L21, len: 106 aa; identical to TR:P95756 (EMBL:D87916) Streptomyces griseus ribosomal protein L21 RplU, 106 aa and highly similar to SW:RL21_ECOLI (EMBL:D13267) Escherichia coli 50S ribosomal protein L21, RplU, 103 aa; fasta scores: opt: 272 z-score: 350.3 E(): 4.3e-12; 38.1% identity in 105 aa overlap. Contains Pfam match to entry PF00829 Ribosomal_L21p, Ribosomal prokaryotic L21 protein 4 2 1 11 [Reporter]SCO4113 (11H15)_PCR/3538 [Gene]SCO4113/3219 NC_003888 SCD17A.05c, conserved hypothetical protein, len: 426 aa; similar to TR:P74590 (EMBL:D90916) Synechocystis sp. hypothetical 43.5 kDa protein SLL1495, 397 aa; fasta scores: opt: 1264 z-score: 1402.4 E(): 0; 49.1% identity in 401 aa overlap 4 2 1 9 [Reporter]SCO3450 (13P11)_PCR/3536 [Gene]SCO3450/3218 NC_003888 SCE46.07c, probable RNA polymerase sigma factor (ECF subfamily), len: 201 aa; similar to TR:O87834 (EMBL:AJ010320) Streptomyces coelicolor RNA polymerase sigma factor SigR, 227 aa; fasta scores: opt: 337 z-score: 413.7 E(): 1.1e-15; 34.1% identity in 208 aa overlap and to SW:RPOE_MYCTU (EMBL:Z95120) Mycobacterium tuberculosis RNA polymerase sigma-E factor (sigma-24), 216 aa; fasta socres: opt: 339 z-score: 416.4 E(): 7.5e-16; 35.4% identity in 189 aa overlap. Contains match to Pfam entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) and match to PS01063 Sigma-70 factors ECF subfamily signature. Contains also a possible helix-turn-helix motif at residues 159..180 (+3.23 SD) 4 2 1 8 [Reporter]SCO3792 (14L11)_PCR/3535 [Gene]SCO3792/3217 NC_003888 SCH63.39, probable methionyl tRNA synthetase, len: 538 aa; similar to SW:SYM_BACST (EMBL:X57925) Bacillus stearothermophilus methionyl tRNA synthetase (EC 6.1.1.10) MetG, 649 aa; fasta scores: opt: 1599 z-score: 1851.5 E(): 0; 46.7% identity in 525 aa overlap. Contains Pfam match to entry PF00133 tRNA-synt_1, tRNA synthetases class I (I, L, M and V) 4 2 1 7 [Reporter]SCO3422 (15H11)_PCR/3534 [Gene]SCO3422/3216 NC_003888 SCE9.29c, hypothetical protein, len: 67 aa; improbable CDS suggested by GC frame plot alone 4 2 1 6 [Reporter]SCO0590 (16D11)_PCR/3533 [Gene]SCO0590/3215 NC_003888 SCF55.14, possible oxidoreductase, len: 289 aa; similar to various hypothetical proteins, e.g. TR:O31570 (EMBL:Z99108) Bacillus subtilis YfhB protein, 293 aa; fasta scores: opt: 400 z-score: 454.6 E(): 6e-18; 32.1% identity in 287 aa overlap and low similarity to SW:PHZF_PSEFL (EMBL:L48616) Pseudomonas fluorescens phenazine biosynthesis protein PhzF oxidoreductase, 278 aa; fasta scores: opt: 225 z-score: 259.7 E(): 4.3e-07; 29.3% identity in 287 aa overlap 4 2 1 5 [Reporter]SCO1727 (17P7)_PCR/3532 [Gene]SCO1727/3214 NC_003888 SCI11.16, conserved hypothetical protein, len: 255 aa; unknown function, similar hypothetical proteins e.g. TR:O67070 (EMBL:AE000714) Aquifex aeolicus hypothetical protein (252 aa), fasta scores; opt: 459 z-score: 574.9 E(): 1.1e-24, 33.0% identity in 230 aa overlap and the N-terminal half of SW:YECE_ECOLI (EMBL:AE000280) Escherichia coli hypothetical protein (272 aa) (29.0% identity in 155 aa overlap). Similar to TR:Q9ZBF5 (EMBL:AL035206) S. coelicolor hypothetical protein (292 aa) (32.2% identity in 283 aa overlap) 4 2 1 4 [Reporter]SCO0085 (18L7)_PCR/3531 [Gene]SCO0085/3213 NC_003888 SCJ11.14c, unknown, len: 163 aa; weak similarity to a region from many proposed DNA ligases eg. TR:CAB45581 (EMBL:AL079355) putative DNA ligase from Streptomyces coelicolor (355 aa) fasta scores; opt: 182, z-score: 228.5, E(): 2.3e-05, (32.6% identity in 132 aa overlap). 3 1 6 19 [Reporter]SCO6608 (4K4)_PCR/1136 [Gene]SCO6608/1039 NC_003888 SC1F2.05, probable secreted protein, len: 552 aa; contains N-terminal signal sequence 4 2 1 3 [Reporter]SCO1374 (19H7)_PCR/3530 [Gene]SCO1374/3212 NC_003888 SC10A9.16, putative secreted protein, len: 203 aa. Contains possible N-terminal region signal peptide sequence 3 1 6 18 [Reporter]SCO6360 (5G4)_PCR/1135 [Gene]SCO6360/1038 NC_003888 SC3A7.28, probable ABC transporter ATP-binding protein, similar to many, e.g. BCRA_BACLI bacitracin transport ATP-binding protein bcrA (306 aa), fasta scores; opt: 540 z-score: 640.6 E(): 2e-28, 34.9% identity in 298 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop) and Pfam match to entry PF00005 ABC_tran, ABC transporters, score 137.30, E-value 2.7e-37 3 2 23 22 [Reporter]SCO4931 (1K23)_PCR/3528 [Gene]SCO4931/3211 NC_003888 SCK13.23, possible membrane protein, len: 366 aa; highly similar to C-terminal region of SW:YHUH_STRGR (EMBL:M77841) Streptomyces griseus hypothetical protein in HutH 5' region (fragment), 241 aa; fasta scores: opt: 1253 z-score: 1461.2 E(): 0; 81.0% identity in 242 aa overlap. Contains Pfam match to entry PF00990 DUF9, Domain of unknown function DUF9. Contains possible hydrophobic membrane spanning region 3 1 6 17 [Reporter]SCO6644 (6C4)_PCR/1134 [Gene]SCO6644/1037 NC_003888 SC4G2.18, probable solute-binding lipoprotein, len: 503 aa; similar to many members of the bacterial extracellular solute-binding protein family 5YLIB_ECOLI putative binding protein YLIB precursor (512 aa), fasta scores; opt: 364 z-score: 328.6 E(): 4.6e-11, 27.8% identity in 467 aa overlap. Contains N-terminal signal sequence with appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site and Pfam match to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5, score 36.60, E-value 2.2e-08 3 2 23 21 [Reporter]SCO0895 (2G23)_PCR/3527 [Gene]SCO0895/3210 NC_003888 SCM1.28c, hrdC, RNA polymerase principal sigma factor HrdC, len: 339 aa. Previously sequenced and characterised Streptomyces coelicolor strain A3(2) SW:HRDC_STRCO(EMBL:X52981). Contains a Pfam match to entry PF00140 sigma70, Sigma-70 factor. The putative helix-turn-helix motif is situated between residues 298..319 (+6.49 SD). 3 1 6 16 [Reporter]SCO4858 (6O24)_PCR/1133 [Gene]SCO4858/1036 NC_003888 SC5G8.26c, dhsC, possible succinate dehydrogenase membrane subunit, len: 126aa; similar to many eg. TR:O53368 (EMBL:AL021841) putative membrane anchor of succinate dehydrogenase from Mycobacterium tuberculosis (112 aa) fasta scores; opt: 484, z-score: 649.2, E(): 1e-28, 65.7% identity in 99 aa overlap. Also wealky similar to SW:P10446 (DHSC_ECOLI) succinate dehydrogenase cytochrome b-556 subunit from Escherichia coli (129 aa) fasta scores; opt: 121, z-score: 173.9, E(): 0.031, 29.6% identity in 71 aa overlap. Contains Pfam match to entry PF01127 Sdh_cyt, Succinate dehydrogenase cytochrome b subunit. 3 1 6 15 [Reporter]SCO2134 (7K24)_PCR/1132 [Gene]SCO2134/1035 NC_003888 SC6G10.07c, unknown, len: 385aa; similar to TR:O53523 (EMBL:AL021957) hypothetical protein from Mycobacterium tuberculosis (257 aa) fasta scores; opt: 225, z-score: 262.9, E(): 2.4e-07, (33.1% identity in 254 aa overlap). 3 1 6 14 [Reporter]SCO5953 (8G24)_PCR/1131 [Gene]SCO5953/1034 NC_003888 SC7H1.23, putative membrane protein, len: 913 aa; weakly similar to many members of the eukaryotic beta-transducin family e.g. YA3A_SCHPO Q09715 hypothetical Trp-Asp repeats containing protein (614 aa), fasta scores; opt: 139 z-score: 352.6 E(): 1.9e-12, 28.8% identity in 250 aa overlap. Contains possible membrane spanning domain around aa 200 and 3x Pfam match to entry G-beta PF00400, WD domain, G-beta repeats, scores; 27.34, 28.55 and 25.74 3 1 6 11 [Reporter]SCO4656 (11K20)_PCR/1128 [Gene]SCO4656/1033 NC_003888 SCD40A.02, possible integral membrane protein, len: 195 aa. Contains possible hydrophobic membrane spanning regions 3 1 6 10 [Reporter]SCO4308 (12G20)_PCR/1127 [Gene]SCO4308/1032 NC_003888 SCD95A.41c, possible transcriptional regulator, len: 320 aa; similar to TR:CAB84834 (EMBL:AL162756) Neisseria meningitidis putative transcriptional regulator NMA1605, 282 aa; fasta scores: opt: 476 z-score: 465.7 E(): 1.8e-18; 33.5% identity in 281 aa overlap and to SW:HEXR_ECOLI (EMBL:AE000279) Escherichia coli hex regulon repressor HexR, 289 aa; fasta scores: opt: 337 z-score: 333.6 E(): 4.1e-11; 26.6% identity in 274 aa overlap. Contains Pfam matches to entries PF01418 HTH_6, Helix-turn-helix domain, rpiR family and PF01380 SIS, SIS domain 3 1 6 9 [Reporter]SCO0681 (13C20)_PCR/1126 [Gene]SCO0681/1031 NC_003888 SCF15.02, possible ferredoxin/ferredoxin-NADP reductase (putative secreted protein), len: 454 aa. Similar to several eukaryotic and prokaryotic ferredoxin-NADP(+) reductases e.g. Rattus norvegicus (Rat) SW:ADRO_RAT (EMBL:D63761) NADPH:adrenodoxin oxidoreductase precursor (EC 1.18.1.2) (494 aa), fasta scores opt: 780 z-score: 902.2 E(): 0 36.3% identity in 463 aa overlap and Mycobacterium leprae TR:O32886 (EMBL:Z98271) possible ferredoxin (456 aa), fasta scores opt: 1030 z-score: 1191.5 E():0 39.2% identity in 454 aa overlap. Contains possible N-terminal region signal peptide sequence 3 1 6 8 [Reporter]SCO0691 (14O16)_PCR/1125 [Gene]SCO0691/1030 NC_003888 SCF42.01, possible oxidoreductase, partial CDS, len: > 369 aa. Similar to several including: Desulfovibrio gigas SW:MOP_DESGI (EMBL:X77222) aldehyde oxidoreductase (907 aa), fasta scores opt: 255 z-score: 288.0 E(): 1.2e-08 28.4% identity in 388 aa overlap and Deinococcus radiodurans TR:AAF12400 (EMBL:AE001863) oxidoreductase (708 aa), fasta scores opt: 1255 z-score: 1421.5 E():0 54.2% identity in 356 aa overlap. Overlaps and extends into CDS SCF15.12 on the adjoining cosmid SCF15. Contains a Pfam match to entry PF01315 Ald_Xan_dh_C, Aldehyde oxidase and xanthine dehydrogenase, C terminus.,SCF15.12, possible oxidoreductase, large subunit, partial CDS, len: > 374 aa. Similar to the C-terminal domain of many eukaryotic xanthine dehydrogenases including: Mus musculus (Mouse) SW:XDH_MOUSE (EMBL:X75129) xanthine dehydrogenase/oxidase (1335 aa), fasta scores opt: 424 z-score: 459.8 E(): 3.1e-18 26.6% identity in 380 aa overlap and Homo sapiens (Human) SW:XDH_HUMAN (EMBL:D11456) xanthine dehydrogenase/oxidase (1332 aa), fasta scores opt: 396 z-score: 429.4 E(): 1.5e-16 25.7% identity in 378 aa overlap. Also similar to Rhodobacter capsulatus (Rhodopseudomonas capsulata) TR:O54051(EMBL:AJ001013) xanthine dehydrogenase (EC 1.1.1.204) (779 aa), fasta scores opt: 452 z-score: 493.7 E(): 4e-20 30.1% identity in 375 aa overlap. Contains a Pfam match to entry PF01315 Ald_Xan_dh_C, Aldehyde oxidase and xanthine dehydrogenase. 3 2 23 20 [Reporter]SCO7399 (3C23)_PCR/3526 [Gene]SCO7399/3209 NC_003888 SC10G8.27c, possible binding-protein-dependent transport lipoprotein, len: 327 aa. Similar to many including: Escherichia coli SW:FHUD_ECOLI(EMBL:X05810) ferrichrome-binding periplasmic protein precursor FhuD (296 aa), fasta scores opt: 231 z-score: 249.9 E(): 1.7e-06 24.8% identity in 270 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains a Pfam match to entry PF01497 Peripla_BP_2, Periplasmic binding protein. 3 2 23 19 [Reporter]SCO7621 (4O19)_PCR/3525 [Gene]SCO7621/3208 NC_003888 SC2H2.19, conserved hypothetical protein, len: 147 aa; low similarity to TR:AAG05206 (EMBL:AE004608) Pseudomonas aeruginosa hypothetical 14.4 kDa protein PA1817, 129 aa; fasta scores: opt: 142 z-score: 193.7 E(): 0.0029; 30.5% identity in 131 aa overlap 3 2 23 18 [Reporter]SCO6713 (5K19)_PCR/3524 [Gene]SCO6713/3207 NC_003888 SC4C6.23, probable lacI-family transcriptional regulator, len: 352 aa; similar to many e.g. TR:O87590 (EMBL:AF086819), CelR, Thermomonospora fusca transcriptional regulator of cellulase genes (340 aa), fasta scores; opt: 991 z-score: 1119.2 E(): 0, 51.9% identity in 324 aa overlap. Similar to others from S. coelicolor e.g. TR:O86795 (EMBL:AL031317) S. coelicolor putative transcriptional regulator (355 aa) (36.3% identity in 342 aa overlap). Contains Pfam matches to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family and to entry PF00356 lacI, Bacterial regulatory proteins, lacI family. Contains probable helix-turn-helix motif at aa 9-30 (Score 2307, +7.04 SD) 3 2 23 17 [Reporter]SCO7565 (6G19)_PCR/3523 [Gene]SCO7565/3206 NC_003888 SC5F1.19, probable oligopeptide ABC transporter integral membrane protein, len: 301 aa; similar to SW:DPPC_ECOLI (EMBL:L08399) Escherichia coli dipeptide transport system permease protein DppC, 300 aa; fasta scores: opt: 805 z-score: 912.6 E(): 0; 45.2% identity in 270 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00038 Myc-type, 'helix-loop-helix' dimerization domain signature. Also contains possible hydrophobic membrane spanning regions 3 2 23 16 [Reporter]SCO2678 (7C19)_PCR/3522 [Gene]SCO2678/3205 NC_003888 SC6D10.21, hypothetical protein, len: 171 aa; similar to TR:CAB55735 (EMBL:AL117387) Streptomyces coelicolor putative secreted protein SCF41.32c, fasta scores: opt: 399 z-score: 488.3 E(): 8.7e-20; 45.6% identity in 169 aa overlap 3 2 23 15 [Reporter]SCO2004 (8O15)_PCR/3521 [Gene]SCO2004/3204 NC_003888 SC7H2.18, possible formate dehydrogenase, len: 759 aa; similar to many formate dehydrogenase e.g. SW:FDHF_ECOLI formate dehydrogenase H from Escherichia coli (715 aa) fasta scores; opt: 386, z-score: 441.8, E(): 2.8e-17, (26.3% identity in 670 aa overlap). Also similar to SW:YX31_MYCTU hypothetical protein from Mycobacterium tuberculosis (779 aa) fasta scores; opt: 3038, z-score: 3497.1, E(): 0, (59.7% identity in 767 aa overlap). 3 2 23 14 [Reporter]SCO5860 (9K15)_PCR/3520 [Gene]SCO5860/3203 NC_003888 SC2E9.01, suhB, probable extragenic suppressor protein, partial CDS, len: >209 aa, similar to eg. SUHB_ECOLI P22783 extragenic suppressor protein Suhb (267 aa), fasta scores; opt: 448 z-score: 565.9 E(): 2.5e-24, 41.0% identity in 212 aa overlap, and to eg. MYO2_LYCES P54927 myo-inositol-1(or 4)-monophosphatase (265 aa), fasta scores; opt: 359 z-score: 450.8 E(): 6.6e-18, 38.7% identity in 199 aa overlap. Contains PS00629 and PS00630 Inositol monophosphatase family signatures 1 and 2 and Pfam match to entry inositol_P PF00459, Inositol monophosphatase family, score 64.03. Overlaps and extends ORF from neighbouring cosmid SC9B10.27,SC9B10.27, suhB gene homolog, partial CDS, len > 96 aa; similar to E.coli SUHB_ECOLI P22783 extragenic suppressor protein Suhb (267 aa), fasta scores; opt: 186 z-score: 333.9 E(): 1.9e-11, 40.0% identity in 85 aa overlap; and to MYOP_HUMAN P29218 myo-inositol-1(or 4)-monophosphatase (277 aa), fasta scores; opt: 180 z-score: 267.3 E(): 9.9e-08, 37.2% identity in 94 aa overlap 3 1 6 7 [Reporter]SCO5138 (15K16)_PCR/1124 [Gene]SCO5138/1029 NC_003888 SCP8.01c, possible membrane protein (fragment), len: >86 aa. . Contains possible hydrophobic membrane spanning region,SC9E12.23c, hypothetical protein (fragment), len: >270 aa; similar to TR:Q9X867 (EMBL:AL049661) Streptomyces coelicolor putative lipoprotein SCE134.12, 381 aa; fasta scores: opt: 432 z-score: 481.0 E(): 2.5e-19; 41.2% identity in 211 aa overlap 3 2 23 13 [Reporter]SCO2436 (10G15)_PCR/3519 [Gene]SCO2436/3202 NC_003888 SCC24.07, possible integral membrane protein, len: 394 aa. Contains possible hydrophobic membrane spanning regions 3 1 6 6 [Reporter]SCO0719 (16G16)_PCR/1123 [Gene]SCO0719/1028 NC_003888 SCF42.29c, hypothetical protein, len: 158 aa; similar to TR:Q9KZJ6 (EMBL:AL353863) Streptomyces coelicolor hypothetical protein SCG8A.03, 156 aa; fasta scores: opt: 570 Z-score: 608.7 E(): 2.8e-26; 59.477% identity in 153 aa overlap 3 2 23 12 [Reporter]SCO4108 (11C15)_PCR/3518 [Gene]SCO4108/3201 NC_003888 SCD17.12, probable peptidase, len: 347 aa. Similar to several Streptomyces coelicolor putative peptidases e.g. TR:CAB39706 (EMBL; AL049485) SC6A5.22 (312 aa), fasta scores opt: 570 z-score: 526.9 E(): 5.4e-22 37.1% identity in 315 aa overlap and TR:CAB40868 (EMBL; AL049628) SCE94.19C (228 aa), fasta scores opt: 460 z-score: 429.8 E(): 1.4e-16 40.1% identity in 217 aa overlap. Contains a Pfam match to entry PF01551 Peptidase_M37, an alanine rich region between residues 163..207 and a probable coiled coil between residues 149..219 (probability 1.00). 3 1 6 5 [Reporter]SCO3158 (17C16)_PCR/1122 [Gene]SCO3158/1027 NC_003888 SCE87.09c, unknown, len: 126 aa. Similar to two putative regulators from Streptomyces coelicolor: TR:CAB51005 (EMBL:AL096852) SCE19A.24 (142 aa), fasta scores opt: 168 z-score: 227.4 E(): 2.7e-05 32.7% identity in 101 aa overlap and TR:CAB40672 (EMBL: AL049587) SC5F2A.05C (138 aa), fasta scores opt: 222 z-score: 296.3 E(): 3.9e-09 36.0% identity in 114 aa overlap. 3 2 23 11 [Reporter]SCO4162 (12O11)_PCR/3517 [Gene]SCO4162/3200 NC_003888 SCD84.29, possible integral membrane protein, len: 437 aa. High content in alanine, proline, glycine and glutamine amino acid residues. Contains possible hydrophobic membrane spanning domains in C-terminal region and degenerate amino acid repeat: 6x (R/P/Q)YDP(N/G) in N-terminal region 3 1 6 4 [Reporter]SCO3635 (18O12)_PCR/1121 [Gene]SCO3635/1026 NC_003888 SCH10.13c, hypothetical protein, len: 133 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 3 1 6 3 [Reporter]SCO1967 (19K12)_PCR/1120 [Gene]SCO1967/1025 NC_003888 SC3C9.02c, possible integral membrane protein, len: 292 aa; similar to TR:Q9KZI4 (EMBL:AL353863) Streptomyces coelicolor putative integral membrane protein SCG8A.15c, 244 aa; fasta scores: opt: 703 Z-score: 726.3 bits: 142.1 E(): 7.6e-33; 48.430% identity in 223 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 6 2 [Reporter]SCP1.233B (20C12)_PCR/1119 [Gene]SCP1.233B/1024 NC_003888 SCP1.233B, mmyC, possible acyl carrier protein synthase, len: 332aa; previously sequenced and annotated as putative condensing protein TR:Q9JN82 (EMBL:AJ276673). Similar to many predicted acyl carrier protein synthases eg. TR:O54151 (EMBL:AL021409) putative 3-oxoacyl-[acyl-carrier-protein] synthase from Streptomyces coelicolor (335 aa) fasta scores; opt: 1184, z-score: 1362.6, E(): 0, 57.8% identity in 332 aa overlap. 3 1 5 22 [Reporter]SCO5249 (1C8)_PCR/1117 [Gene]SCO5249/1023 NC_003888 2SC7G11.11, possible nucleotide-binding protein, len: 468 aa; highly similar to TR:Q54255 (EMBL:L76204) Streptomyces griseus sporulation protein, 470 aa; fasta scores: opt: 2008 z-score: 2272.9 E(): 0; 64.1% identity in 462 aa overlap. Contains Pfam match to entry PF00027 cNMP_binding, Cyclic nucleotide-binding domain 3 1 5 21 [Reporter]SCO2833 (2O4)_PCR/1116 [Gene]SCO2833/1022 NC_003888 SCE20.07c, chb, secreted chitin binding protein, len: 201 aa. Highly similar to several including: Streptomyces olivaceoviridis (Streptomyces corchorusii) TR:Q54501(EMBL:X78535) chitin binding protein precursor Chb1 (201 aa), fasta scores opt: 1185 z-score: 1303.0 E():0 83.7% identity in 202 aa overlap and Streptomyces reticuli TR:O87962(EMBL:Y14315) chitin binding protein (Chb2) (201 aa), fasta scores opt: 1161 z-score: 1276.8 E(): 0 79.1% identity in 201 aa overlap. Contains a possible N-terminal signal sequence. 3 1 5 20 [Reporter]SCO4730 (3K4)_PCR/1115 [Gene]SCO4730/1021 NC_003888 SC6G4.08, rplQ, 50S ribosomal protein L17, len: 168 aa; highly similar to many e.g. RL17_BACST 50S ribosomal protein L17 (119 aa), fasta scores; opt: 339 z-score: 597.2 E (): 5.3e-26, 47.4% identity in 116 aa overlap. Contains approx 50 aa C-terminal extension, not present in other RplQ 3 1 5 19 [Reporter]SCO6917 (4G4)_PCR/1114 [Gene]SCO6917/1020 NC_003888 SC1B2.23c, unknown, len: 162 aa. 3 1 5 18 [Reporter]SCO6355 (5C4)_PCR/1113 [Gene]SCO6355/1019 NC_003888 SC3A7.23c, probable integral membrane protein, len: 272 aa. Contains possible hydrophobic membrane spanning regions 3 1 5 17 [Reporter]SCO7326 (5O24)_PCR/1112 [Gene]SCO7326/1018 NC_003888 SC4G10.05c, unknown, len: 824 aa. Similar to several Streptomyces coelicolor proteins of undefined function e.g. TR:CAB61192(EMBL:AL132973) SCF91.35 (884 aa), fasta scores opt: 385 z-score: 407.5 E(): 3.1e-15 26.1% identity in 885 aa overlap 3 1 5 16 [Reporter]SCO4852 (6K24)_PCR/1111 [Gene]SCO4852/1017 NC_003888 SC5G8.20c, possible integral membrane protein, len: 135 aa. Contains possible hydrophobic membrane spanning regions 3 1 5 15 [Reporter]SCO1430 (7G24)_PCR/1110 [Gene]SCO1430/1016 NC_003888 SC6D7.09, possible TetR-family transcriptional regulator, len: 220 aa. Weakly similar to several putative regulatory proteins e.g. Streptomyces coelicolor TR:CAB55659(EMBL:AL117385) putative tetR-family transcriptional regulator SC5G9.11C (197 aa), fasta scores opt: 164 z-score: 201.8 E(): 0.00073 26.2% identity in 145 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 44..65 (+3.74 SD). 3 1 5 14 [Reporter]SCO5949 (8C24)_PCR/1109 [Gene]SCO5949/1015 NC_003888 SC7H1.19, unknown, len: 182 aa 3 1 5 13 [Reporter]SCO6458 (9O20)_PCR/1108 [Gene]SCO6458/1014 NC_003888 SC9B5.25, unknown, len: 292aa; similar to TR:O32135 (EMBL:Z99120) hypothetical protein from Bacillus subtilis (284 aa) fasta scores: opt: 304, z-score: 523.6, E(): 7.2e-22, (32.1% identity in 293 aa overlap). 3 1 5 11 [Reporter]SCO4069 (11G20)_PCR/1106 [Gene]SCO4069/1013 NC_003888 SCD25.05, possible integral membrane protein, len: 664 aa. Contains a degenerate direct repeat region: residues 469_ PYADDEA P HAPYADDEA L HVPDADDEA P HAPYAD_502. Also contains possible membrane spanning hydrophobic domains. 3 1 5 10 [Reporter]SCO4304 (12C20)_PCR/1105 [Gene]SCO4304/1012 NC_003888 SCD95A.37, probable oxidoreductase, len: 249 aa; similar to TR:CAB71826 (EMBL:AL138662) Streptomyces coelicolor putative dehydrogenase/reductase SC8E4A.21c, 245 aa; fasta scores: opt: 757 z-score: 795.6 E(): 0; 50.0% identity in 250 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 3 1 5 9 [Reporter]SCO5147 (13O16)_PCR/1104 [Gene]SCO5147/1011 NC_003888 SCP8.10, probable ECF-subfamily sigma factor, len: 223 aa; similar to TR:O05735 (EMBL:U87308) Mycobacterium avium extracytoplasmic function alternative sigma factor SigE, 251 aa; fasta scores: opt: 852 z-score: 1013.1 E(): 0; 62.5% identity in 216 aa overlap and to SW:CARQ_MYXXA (EMBL:X71062) Myxococcus xanthus RNA polymerase sigma factor CarQ, 174 aa; fasta scores: opt: 285 z-score: 347.6 E(): 6.8e-12; 36.5% identity in 159 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily). Also contains a possible helix-turn-helix motif at residues 164..185 (+3.76 SD) 3 1 5 8 [Reporter]SCO0948 (14K16)_PCR/1103 [Gene]SCO0948/1010 NC_003888 SCM11.03c, possible alpha-mannosidase, len: 1007 aa; similar to TR:AAC00190 (EMBL:AF044414) Homo sapiens alpha-mannosidase 6A8B, 1062 aa; fasta scores: opt: 1326 z-score: 1446.5 E(): 0; 37.8% identity in 1013 aa overlap and to SW:MAN1_RAT (EMBL:M57547) Rattus norvegicus alpha-mannosidase (EC 3.2.1.24), 1040 aa; fasta scores: opt: 1285 z-score: 1401.8 E(): 0; 35.9% identity in 1041 aa overlap 3 3 20 22 [Reporter]SCO1342 (1O18)_PCR/5479 [Gene]SCO1342/4999 NC_003888 2SCG61.24c, unknown, len: 183aa; 3 3 20 21 [Reporter]SCO5257 (2K18)_PCR/5478 [Gene]SCO5257/4998 NC_003888 2SC7G11.19, metZ, methyltransferase, len: 269 aa; previously sequenced as TR:Q9RGW5 (EMBL:AF104994) Streptomyces coelicolor A3(2) methyltransferase MetZ, 268 aa 3 3 20 20 [Reporter]SCO7651 (3G18)_PCR/5477 [Gene]SCO7651/4997 NC_003888 SC10F4.24c, probable tetR-family transcriptional regulator, len: 204 aa; similar to TR:O34381 (EMBL:Z99112) Bacillus subtilis transcriptional regulator PksA, 205 aa; fasta scores: opt: 246 z-score: 297.6 E(): 4.9e-09; 27.5% identity in 182 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 38..59 (+2.87 SD) 3 3 20 19 [Reporter]SCO5605 (4C18)_PCR/5476 [Gene]SCO5605/4996 NC_003888 SC2E1.22, unknown prophage gene, len: 103 aa 3 3 20 18 [Reporter]SCO7678 (5O14)_PCR/5475 [Gene]SCO7678/4995 NC_003888 SC4C2.13, possible metal transport integral membrane protein, len: 316 aa; similar to SW:NIKB_ECOLI (EMBL:X73143) Escherichia coli nickel transport system permease protein NikB, 314 aa; fasta scores: opt: 490 z-score: 547.6 E(): 5.8e-23; 30.4% identity in 313 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Also contains possible hydrophobic membrane spanning regions and possible N-terminal signal peptide sequence 3 3 20 17 [Reporter]SCO6748 (6K14)_PCR/5474 [Gene]SCO6748/4994 NC_003888 SC5F2A.31c, possible enoyl coA hydratase, len: 257aa; similar to many proposed to be enoyl coA hydratase based on Pfam match eg. TR:P97087 (EMBL:Z92974) from Clostridium thermosaccharolyticum (259 aa) fasta scores; opt: 426, z-score: 496.6, E(): 2.4e-20, (31.2% identity in 247 aa overlap). Contains Pfam match to entry PF00378 ECH, Enoyl-CoA hydratase/isomerase family. 3 1 5 6 [Reporter]SCO2888 (16C16)_PCR/1101 [Gene]SCO2888/1009 NC_003888 SCE6.25c, unknown, len: 119 aa. similar to TR:CAC46858 (EMBL:AL591790) hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (120 aa) fasta scores; opt: 360, Z-score: 449.2, E(): 2.2e-17, 48.305% identity (49.565% ungapped) in 118 aa overlap. Weakly similar in parts to Streptomyces coelicolor TR:O86701(EMBL:AL031515) putative lyase, SC5C7.04 (138 aa), fasta scores opt: 141 z-score: 193.4 E(): 0.0024 33.3% identity in 111 aa overlap. 3 3 20 16 [Reporter]SCO5629 (7G14)_PCR/5473 [Gene]SCO5629/4993 NC_003888 SC6A9.38, unknown ATP /GTP-binding protein, len: 886 aa; C-terminus contains 7 x 42 aa repeats with weak similarity to eukaryotic kinesin light chain 42 aa repeats e.g. KNLC_LOLPE kinesin light chain (klc) (571 aa), fasta scores; opt: 302 z-score: 260.7 E(): 2.8e-07, 24.0% identity in 405 aa overlap. Contains PS00017 ATP/GTP- binding 3 1 5 5 [Reporter]SCO0144 (17O12)_PCR/1100 [Gene]SCO0144/1008 NC_003888 SCJ33.08, unknown, len: 119 aa. 3 3 20 15 [Reporter]SCO5218 (8C14)_PCR/5472 [Gene]SCO5218/4992 NC_003888 SC7E4.15, possible integral membrane protein, len: 441 aa; similar to TR:Q9X1H8 (EMBL:AE001797) Thermotoga maritima conserved hypothetical protein TM1467, 426 aa; fasta scores: opt: 471 z-score: 536.1 E(): 2.2e-22; 29.0% identity in 352 aa overlap. Contains possible N-terminal signal peptide sequence and possible hydrophobic membrane spanning regions 3 1 5 3 [Reporter]SCO1879 (19G12)_PCR/1098 [Gene]SCO1879/1007 NC_003888 SCI39.26c, probable secreted pectinesterase, len: 381 aa; similar to SW:PME_BURSO (EMBL:M62803) Burkholderia solanacearum pectinesterase precursor (EC 3.1.1.11) Pme, 396 aa; fasta scores: opt: 350 Z-score: 372.6 bits: 77.7 E(): 3.7e-13; 32.036% identity in 334 aa overlap. Contains Pfam match to entry PF01095 Pectinesterase, Pectinesterase and match to Prosite entry PS00503 Pectinesterase signature 2. Contains also possible N-terminal region signal peptide sequence 3 3 20 14 [Reporter]SCO7006 (9O10)_PCR/5471 [Gene]SCO7006/4991 NC_003888 SC8F11.32, possible oxidoreductase, len: 416 aa. Similar to several e.g. Mycobacterium smegmatis SW:TRXB_MYCSM(EMBL:AF023161) thioredoxin reductase (EC 1.6.4.5) (311 aa), fasta scores opt: 323 z-score: 335.5 E(): 2.9e-11 30.6% identity in 314 aa overlap. 3 1 4 22 [Reporter]SCO4201 (1O4)_PCR/1095 [Gene]SCO4201/1006 NC_003888 2SCD46.15c, unknown, len: 544 aa; regions similar to many from Streptomyces coelicolor of undefined function e.g. TR:Q9RD37 (EMBL:AL133422) hypothetical protein (445 aa) fasta scores; opt: 887, z-score: 857.7, E(): 0, 41.4% identity in 423 aa overlap and TR:Q9X8X3 (EMBL:AL078610) putative regulatory protein (422 aa) fasta scores; opt: 325, z-score: 319.4, E(): 2.6e-10, 32.4% identity in 290 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain 3 3 20 13 [Reporter]SCO2568 (10K10)_PCR/5470 [Gene]SCO2568/4990 NC_003888 SCC123.06c, possible DNA-binding protein, len: 355 aa. Similar to Mycobacterium tuberculosis TR:P71728 (EMBL:Z81368) hypothetical 30.5 KD protein (297 aa), opt: 271 z-score: 260.6 E(): 4e-07 36.3% identity in 292 aa overlap. Also similar in parts to Bacillus subtilis SW:CME1_BACSU (EMBL:L15202) ComE operon protein 1, required for the binding and uptake of transforming DNA (205 aa), fasta scores opt: 295 z-score: 284.5 E(): 1.9e-08 32.2% identity in 180 aa overlap. Contains a Pfam match to entry PF00633 HHH, Helix-hairpin-helix motif and a possible membrane spanning hydrophobic domain. 3 1 4 21 [Reporter]SCO5959 (2K4)_PCR/1094 [Gene]SCO5959/1005 NC_003888 SC7H1.29c, cbiQ, probable cobalt transport integral membrane protein, len: 249 aa; similar to eg. CBIQ_SALTY Q05598 cobalt transport protein cbiq (225 aa), fasta scores; opt: 182 z-score: 241.4 E(): 3e-06, 28.1% identity in 228 aa overlap 3 1 4 19 [Reporter]SCO6913 (4C4)_PCR/1092 [Gene]SCO6913/1004 NC_003888 SC1B2.19, unknown, len: 117 aa. 3 1 4 18 [Reporter]SCO7090 (4O24)_PCR/1091 [Gene]SCO7090/1003 NC_003888 SC3A4.16c, possible integral membrane protein, len: 748 aa. Highly similar to several including: Streptomyces coelicolor TR:CAB59662(EMBL:AL132674) putative integral membrane transport protein, SCE87.17C (745 aa), fasta scores opt: 2918 z-score: 3028.3 E():0 61.8% identity in 744 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 3 1 4 17 [Reporter]SCO7322 (5K24)_PCR/1090 [Gene]SCO7322/1002 NC_003888 SC4G10.01c, possible anti-sigma factor, partial CDS, len: >57 aa. Highly similar to several including: Bacillus subtilis SW:RSBT_BACSU (EMBL:L35574) anti-sigma B factor controlling SigB the general stress sigma factor (133 aa), fasta scores opt: 144 z-score: 235.0 E(): 1.3e-05 48.0% identity in 50 aa overlap and Deinococcus radiodurans TR:AAF12580(EMBL:AE001826) sigma-B regulator, RsbT (137 aa), fasta scores opt: 201 z-score: 321.3 E(): 2e-10 63.2% identity in 57 aa overlap.,SC5F8.32c, possible anti-sigma factor, partial CDS, len: >129 aa. Highly similar to several anti-sigma factors including: Bacillus subtilis SW:RSBT_BACSU(EMBL:) anti-sigma B factor, RsbT (133 aa), fasta scores opt: 452 z-score: 536.7 E(): 2e-22 57.6% identity in 118 aa overlap. Overlaps and extends into CDS SC4G10.01c on the adjoining cosmid. 3 1 4 16 [Reporter]SCO6736 (6G24)_PCR/1089 [Gene]SCO6736/1001 NC_003888 SC5F2A.19c, possible metallopeptidase, len: 506aa; similar to many egs. TR:O69971 (EMBL:AL022268) proposed zinc metallopeptidase from Streptomyces coelicolor (512 aa) fasta scores; opt: 934, z-score: 1039.4, E(): 0, (36.3% identity in 509 aa overlap) and TR:G4063364 (EMBL:AF081952) from Thermoplasma acidophilum (780 aa) fasta scores; opt: 387, z-score: 428.8, E(): 1.4e-16, (28.2% identity in 447 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. 3 1 4 15 [Reporter]SCO1434 (7C24)_PCR/1088 [Gene]SCO1434/1000 NC_003888 SC6D7.05c, possible CbxX/CfqX family protein, len: 618 aa. Similar to several proteins of unknown function e.g. Mycobacterium tuberculosis TR:O69733(EMBL:AL022120) hypothetical 62.4 KD protein (573 aa),fasta scores opt: 751 z-score: 757.0 E():0 31.0% identity in 590 aa overlap. The C-terminus is similar to many proteins of the CbxX/CfqX family e.g. Bacillus subtilis SW:SP5K_BACSU(EMBL:X59412) stage V sporulation protein K (322 aa), fasta scores opt: 645 z-score: 654.4 E(): 4.5e-29 39.6% identity in 280 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). Also contains a TTA /leucine codon, possible target for bldA regulation. 3 3 20 12 [Reporter]SCO2496 (11G10)_PCR/5469 [Gene]SCO2496/4989 NC_003888 SCC82.02, possible secreted protein, len: 146 aa. Contains possible N-terminal region signal peptide sequence 3 3 20 11 [Reporter]SCO4166 (12C10)_PCR/5468 [Gene]SCO4166/4988 NC_003888 SCD66.03, possible integral membrane protein, len: 97 aa. Contains possible hydrophobic membrane spanning regions 3 3 20 10 [Reporter]SCO3275 (13O6)_PCR/5467 [Gene]SCO3275/4987 NC_003888 SCE39.25c, possible merR-family transcriptional regulator, len: 150 aa; similar to many e.g. SW:MERR_BACSR MerR, mercury resistance operon transcriptional regulator from Bacillus subtilis (132 aa) fasta scores; opt: 201, z-score: 245.2, E(): 2.4e-06, (40.3% identity in 77 aa overlap) and SW:TIPA_STRLI TipA, transcriptional activator of a thiostrepton-induced gene in Streptomyces lividans (253 aa) fasta scores; opt: 196, z-score: 235.4, E(): 8.4e-06, (35.5% identity in 107 aa overlap). Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and Prosite match to PS00552 Bacterial regulatory proteins, merR family signature. Also contains probable helix-turn-helix (+3.27 SD) 13-34 aa 3 3 20 9 [Reporter]SCO3366 (14K6)_PCR/5466 [Gene]SCO3366/4986 NC_003888 SCE94.17c, possible exporter, len: 531aa; similar to many export proteins eg. TR:G4240426 (EMBL:AF080235) antibiotic transporter landomycin biosynthesis cluster of Streptomyces cyanogenus S136 (517 aa) fasta scores; opt: 1848, z-score: 1973.8, E(): 0, (55.2% identity in 511 aa overlap). also similar to TR:E1370405 (EMBL:AL035161) putative efflux protein from Streptomyces coelicolor (815 aa) fasta scores; opt: 1622, z-score: 1730.4, E(): 0, (46.1% identity in 518 aa overlap). Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter. Also contains several probable membrane spanning hydrophobic regions. 3 3 20 8 [Reporter]SCO3692 (15G6)_PCR/5465 [Gene]SCO3692/4985 NC_003888 SCH35.32c, possible anti-sigma factor antagonist, len: 119aa; similar to many egs. TR:CAB38486 (EMBL:AL035636) putative anti-sigma factor antagonist from Streptomyces coelicolor (113 aa) fasta scores; opt: 218, z-score: 288.7, E(): 9.4e-09, (34.9% identity in 109 aa overlap) and SW:SP21_BACSU anti-sigma F factor antagonist from Bacillus subtilis (116 aa) fasta scores; opt: 152, z-score: 206.5, E(): 0.00036, (30.9% identity in 94 aa overlap). 3 3 20 7 [Reporter]SCO3129 (16C6)_PCR/5464 [Gene]SCO3129/4984 NC_003888 SCE66.08, possible TetR-family transcriptional regulator, len: 225 aa; similar to many proposed transcriptional regulators e.g. TR:Q9RYK4 (EMBL:AE001863) transcriptional regulator, TetR-family from Deinococcus radiodurans (239 aa) fasta scores; opt: 318, z-score: 386.8, E(): 4.6e-14, 32.7% identity in 208 aa overlap. Also similar to TR:P96381 (EMBL:Z92539) hypothetical protein from Mycobacterium tuberculosis (197 aa) fasta scores; opt: 827, z-score: 991.9, E(): 0, 64.4% identity in 194 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and helix-turn-helix motif (Score 1473 (+4.20 SD) in SCE66.08 at residues 51-72 3 3 20 6 [Reporter]SCO0395 (17O2)_PCR/5463 [Gene]SCO0395/4983 NC_003888 SCF62.21, possible epimerase/dehydratase, len: 314 aa. Highly similar to Streptomyces glaucescens TR:Q54262 (EMBL:X78974) putative protein involved in biosynthesis of 5'-hydroxystreptomycin StrP (358 aa), fasta scores opt: 1446 z-score: 1656.6 E(): 0 68.4% identity in 342 aa overlap. Also similar to Streptomyces griseus SW:STRE_STRGR (EMBL:X62567) DTDP-glucose 4,6-dehydratase (EC 4.2.1.46) (328 aa), fasta scores opt: 260 z-score: 303.2 E(): 1.6e-09 28.4% identity in 317 aa overlap. Contains a Pfam match to entry PF01370 Epimerase, NAD dependent epimerase/dehydratase family 3 3 20 5 [Reporter]SCO2941 (18K2)_PCR/5462 [Gene]SCO2941/4982 NC_003888 SCE56.05c, unknown, len: 773 aa; similar to C-terminal region of TR:Q9VYQ2 (EMBL:AE003488) Drosophila melanogaster CG2779 protein, 1612 aa; fasta scores: opt: 651 Z-score: 464.7 E(): 3e-18; 31.405% identity in 847 aa overlap. High content in proline, glycine and alanine. Contains Pfam matches to entries PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains and PF01799 fer2_2, [2Fe-2S] binding domain and match to Prosite entry PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature 3 3 20 4 [Reporter]SCO6002 (19G2)_PCR/5461 [Gene]SCO6002/4981 NC_003888 SCBAC1C11.05c, putative membrane protein, len: 142 aa. Contains possible hydrophobic membrane spanning regions 3 3 20 3 [Reporter]SCO4032 (20C2)_PCR/5460 [Gene]SCO4032/4980 NC_003888 2SC10A7.36c, probable marR regulatory protein, len: 166 aa; similar to SW:MARR_ECOLI (EMBL:M96235) Escherichia coli multiple antibiotic resistance protein MarR, 125 aa; fasta scores: opt: 164 z-score: 211.9 E(): 0.00027; 27.3% identity in 110 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 3 3 20 2 [Reporter]SCO2818 (20K22)_PCR/5459 [Gene]SCO2818/4979 NC_003888 SCBAC17F8.09, hypothetical protein, len: 436 aa: similar TR:Q9Z4X3 (EMBL:AL035654) putative secreted protein SCE8.01 from Streptomyces coelicolor (432 aa) fasta scores; opt: 555, Z-score: 609.9, 33.991% identity (38.945% ungapped) in 456 aa overlap 3 3 19 22 [Reporter]SCO1338 (1K18)_PCR/5457 [Gene]SCO1338/4978 NC_003888 2SCG61.20c, possible monooxygenase (putative secreted protein), len: 396 aa; similar to many eg. TR:Q9Z4Y6 (EMBL:AL035707) putative salicylate hydroxylase from Streptomyces coelicolor (420 aa) fasta scores; opt: 505, z-score: 556.8, E(): 1.5e-23, 34.8% identity in 391 aa overlap and TR:Q9ZSN7 (EMBL:AF098947) CTF2B from Arabidopsis thaliana (Mouse-ear cress) (428 aa) fasta scores; opt: 464, z-score: 511.9, E(): 4.9e-21, 30.8% identity in 383 aa overlap. Contains Pfam match to entry PF01494 FAD_binding_3, FAD binding domain and Pfam match to entry PF01360 Monooxygenase, Monooxygenase. Contains possible N-terminal region signal peptide sequence 3 3 19 21 [Reporter]SCO4762 (2G18)_PCR/5456 [Gene]SCO4762/4977 NC_003888 SC6G4.40, groEL1, 60 kD chaperonin cpn60, len: 541 a a; 99.6% identical to CH61_STRCO (539 aa) and highly simila r to many e.g.CH60_BACSU (543 aa), fasta scores; opt: 2152 z-score: 2429.5 E(): 0, 62.6% identity in 537 aa overlap. C ontains PS00296 Chaperonins cpn60 signature and Pfam match to entry PF00118 cpn60_TCP1, Chaperonins cpn60 and TCP-1, s core 759.20, E-value 1.7e-224 3 3 19 20 [Reporter]SCO7647 (3C18)_PCR/5455 [Gene]SCO7647/4976 NC_003888 SC10F4.20, probable calcium binding protein, len: 179 aa; similar to SW:CBP_SACER (EMBL:M29700) Saccharopolyspora erythraea calerythrin (calcium-binding protein), 177 aa; fasta scores: opt: 216 z-score: 256.4 E(): 9.6e-07; 25.5% identity in 165 aa overlap. Contains 3x Pfam matches to entry PF00036 efhand, EF hand and 2x matches to Prosite entry PS00018 EF-hand calcium-binding domain 3 3 19 19 [Reporter]SCO7212 (4O14)_PCR/5454 [Gene]SCO7212/4975 NC_003888 SC2H12.11c, possible secreted glycosyl hydrolase, len: 962 aa. Similar to many including: Arthrobacter sp. SW:E13B_ARTSP(EMBL:D23668) glucan endo-1,3-beta-glucosidase precursor (EC 3.2.1.39) (548 aa), fasta scores opt: 455 z-score: 456.5 E(): 5.9e-18 29.8% identity in 544 aa overlap. Also similar to the N-terminus of Streptomyces coelicolor TR:O69947(EMBL:AL023862) hypothetical 133.5 kd protein, SC3F9.07 (1238 aa), fasta scores opt: 3612 z-score: 3597.9 E(): 0 63.9% identity in 831 aa overlap. Note, the last 131 aa of this CDS are identical to those in the analogous position on the adjoining CDS, SC2H12.10c. Contains a possible N-terminal signal sequence. Contains a Pfam match to entry PF00652 Ricin_B_lectin, Similarity to lectin domain of ricin beta-chain, 3 copies. Contains a TTA leucine 3 3 19 18 [Reporter]SCO7674 (5K14)_PCR/5453 [Gene]SCO7674/4974 NC_003888 SC4C2.09, possible secreted metal-binding protein, len: 132 aa; similar to SW:AMCY_METEX (EMBL:M57963) Methylobacterium extorquens amicyanin-alpha precursor MauC, 119 aa; fasta scores: opt: 165 z-score: 185.2 E(): 0.0088; 29.8% identity in 121 aa overlap. Contains Pfam match to entry PF00127 copper-bind, Copper binding proteins, plastocyanin/azurin family and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and match to PS00017 ATP/GTP-binding site motif A (P-loop). Also contains possible N-terminal region signal peptide sequence 3 3 19 17 [Reporter]SCO7765 (6G14)_PCR/5452 [Gene]SCO7765/4973 NC_003888 SC5E9.13, possible transcriptional regulator, len: 146 aa; weak similar to many e.g. SW:P27245 (MARR_ECOLI) multiple antibiotic resistance protein MarR from Escherichia coli (125 aa) fasta scores; opt: 138, z-score: 184.9, E(): 0.0079, 26.5% identity in 117 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family and helix-turn-helix motif (Score 1478 (+4.22 SD)) at residue 43-64. 3 3 19 16 [Reporter]SCO5637 (7C14)_PCR/5451 [Gene]SCO5637/4972 NC_003888 SC6A9.30c, mutT-like protein, len: 131 aa; almost identical to TR:E1286464 (EMBL:AJ005260) ORF131 from Streptomyces ambofaciens plasmid pSAM2 (131 aa); 96.2% identity in 131 aa overlap, and similar to e.g. MUTT_PROVU mutator mutt protein (7,8-dihydro-8-oxoguanine-triphosphatase) (112 aa), fasta scores; opt: 128 z-score: 217.1 E(): 7.4e-05, 41.8% identity in 55 aa overlap. Contains PS00893 mutT domain signature and Pfam match to entry PF00293 mutT, Bacterial mutT protein, score 58.30, E-value 3e-15 3 2 3 7 [Reporter]SCO3214 (15O11)_PCR/3074 [Gene]SCO3214/2799 NC_003888 SCE8.07c, trpE2, anthranilate synthase component I, len: 511aa; similar to many eg. SW:TRPE_PSEPU TrpE, anthranilate synthase component I from Pseudomonas putida (493 aa) fasta scores; opt: 1173, z-score: 1253.8, E(): 0, (44.3% identity in 492 aa overlap). Contains Pfam match to entry PF00425 chorismate_bind, chorismate binding enzyme, score 529.40, E-value 2.5e-155. 3 3 19 15 [Reporter]SCO6011 (8O10)_PCR/5450 [Gene]SCO6011/4971 NC_003888 SC7B7.08, probable ABC-type trans- membrane transport protein, len: 430 aa; similar to many others of the ARAH/RBSC subfamily of the binding-protein dependent transport systems eg. XYLH_ECOLI P37389 xylose transport permease protein xylH (393 aa), fasta scores; opt: 695 z-score: 690.3 E(): 2.7e-31, 34.2% identity in 377 aa overlap. Possible alternative start site 33 aa downstream 3 2 3 6 [Reporter]SCO5254 (16K11)_PCR/3073 [Gene]SCO5254/2798 NC_003888 2SC7G11.16c, sodN, superoxide dismutase, len: 131 aa; previously sequenced as TR:O51921 (EMBL:AF104994) Streptomyces coelicolor superoxide dismutase (EC 1.15.1.1) SodN, 131 aa 3 3 19 14 [Reporter]SCO7002 (9K10)_PCR/5449 [Gene]SCO7002/4970 NC_003888 SC8F11.28c, probable amino acid/metabolite permease, len: 504 aa. Highly similar to many Eukaryotic and Prokaryotic permeases including: Saccharomyces cerevisiae SW:UGA4_YEAST(EMBL:X66472) GABA-specific permease (571 aa), fasta scores opt: 583 z-score: 659.1 E(): 2.8e-29 30.8% identity in 510 aa overlap and Streptomyces coelicolor TR:CAB56378(EMBL:AL118515) possible amino acid/metabolite permease (fragment), SCD17.01 (389 aa), fasta scores opt: 1521 z-score: 1718.0 E():0 62.7% identity in 359 aa overlap. Contains a Prosite hit to PS00218 Amino acid permeases signature and a Pfam match to entry PF00324 aa_permeases, Amino acid permease. Also contains multiple possible membrane spanning hydrophobic domains. 3 2 3 5 [Reporter]SCO1181 (17G11)_PCR/3072 [Gene]SCO1181/2797 NC_003888 SCG11A.12, hypothetical protein, len:284 aa; similar to TR:CAB46974 (EMBL:AL096825) Streptomyces coelicolor hypothetical 31.8 KD protein SC6G3.02, 292 aa; fasta scores: opt: 864 z-score: 1038.3 E(): 0; 47.9% identity in 286 aa overlap and to TR:O52845 (EMBL:AJ223073) Bradyrhizobium japonicum phosphate regulatroy protein, PhoB, 235 aa; fasta scores: opt: 113 z-score: 142.6 E(): 1.4; 32.1% identity in 131 aa overlap 3 2 3 4 [Reporter]SCO0425 (18C11)_PCR/3071 [Gene]SCO0425/2796 NC_003888 SCF51A.03c, unknown, len: 316 aa. Similar to Streptomyces coelicolor TR:CAB46385 (EMBL; AL096743) hypothetical 30.5 KD protein SCI7.02C (281 aa), fasta scores opt: 424 z-score: 490.6 E(): 5.7e-20 36.6% identity in 292 aa overlap. 3 2 3 3 [Reporter]SCP1.113 (19O7)_PCR/3070 [Gene]SCP1.113/2795 NC_003888 SCP1.113, unknown, len: 255aa; contains a region conserved in other proteins eg. SW:P36892 (YREP_STRAM) hypothetical 19.3 kDa protein in RepSA 5'region (ORF183) from plasmid pSAM2 of Streptomyces ambofaciens, possibly involved in control of plasmid replication (183 aa) fasta scores; opt: 458, z-score: 523.4, E(): 1.1e-21, 47.7% identity in 174 aa overlap. Contains Pfam match to entry PF00990 DUF9, Domain of unknown function DUF9. 3 2 3 2 [Reporter]SCO4886 (20G7)_PCR/3069 [Gene]SCO4886/2794 NC_003888 2SCK8.12, probable sugar ABC transporter ATP-binding protein, len: 537 aa; similar to SW:MGLA_ECOLI (EMBL:M59444) Escherichia coli galactoside transport ATP-binding protein MglA, 506 aa; fasta scores: opt: 1019 z-score: 1013.7 E(): 0; 35.6% identity in 486 aa overlap. Contains 2x Pfam matches to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 2 2 22 [Reporter]SCO5199 (1G3)_PCR/3067 [Gene]SCO5199/2793 NC_003888 2SC3B6.23c, conserved hypothetical protein, len: 487 aa; similar to TR:O53341 (EMBL:AL021646) Mycobacterium tuberculosis hypothetical 49.4 kD protein, 472 aa; fasta scores: opt: 1382 z-score: 1331.8 E(): 0; 46.2% identity in 489 aa overlap 3 2 2 21 [Reporter]SCO0738 (2C3)_PCR/3066 [Gene]SCO0738/2792 NC_003888 3SC5B7.16, possible secreted protein, len: 179 aa. Contains possible N-terminal region signal peptide sequence 3 2 2 20 [Reporter]SCO4564 (2O23)_PCR/3065 [Gene]SCO4564/2791 NC_003888 SCD16A.19c, nuoC, NADH dehydrogenase subunit, len: 251 aa; similar to many e.g. TR:P95179 (EMBL:Z83867) NuoC, NADH dehydrogenase subunit from Mycobacterium tuberculosis (236 aa) fasta scores; opt: 1113, z-score: 1265.4, E(): 0, (67.4% identity in 236 aa overlap) and SW:NUOC_ECOLI, NADH dehydrogenase subunit NuoC from Escherichia coli (183 aa) fasta scores; opt: 350, z-score: 406.4, E(): 2.6e-15, (37.5% identity in 168 aa overlap). Contains Pfam match to entry PF00329 complex1_30Kd, Respiratory-chain NADH dehydrogenase, 30 Kd subunit. 3 2 2 19 [Reporter]SCO6140 (3K23)_PCR/3064 [Gene]SCO6140/2790 NC_003888 SC1A9.04, probable luxR family two-component system response regulator, len: 219 aa; similar to a family of regulators egs. TR:O69816 (EMBL:AL023496) probable two-component regulator from Streptomyces coelicolor (224 aa) fasta scores; opt:714, z-score:1103.5, E():0, (54.8% identity in 219 aa overlap) and NARL_ECOLI (EMBL:X13360) nitrate /nitrite response regulator from Escherichia coli (216 aa) fasta scores; opt: 465, z-score: 656.5, E(): 2.7e-29, (37.6% identity in 213 aa overlap). Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain, score 111.10, E-value 2.1e-29, Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 79.50, E-value 7e-20, PS00622 Bacterial regulatory proteins, luxR family signature and an helix-turn-helix motif from: 1 to: 219, Score 983 (+2.53 SD). 3 3 19 13 [Reporter]SCO7373 (10G10)_PCR/5448 [Gene]SCO7373/4969 NC_003888 SC9H11.27c, unknown, len: 163 aa. 3 3 19 12 [Reporter]SCO2622 (11C10)_PCR/5447 [Gene]SCO2622/4968 NC_003888 SCC80.07, possible integral membrane protein, len: 389 aa; similar to TR:O31660 (EMBL:Z99111) Bacillus subtilis YkrP protein, 340 aa; fasta scores: opt: 590 z-score: 707.1 E(): 7.2e-32; 32.0% identity in 341 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 19 11 [Reporter]SCO3957 (12O6)_PCR/5446 [Gene]SCO3957/4967 NC_003888 SCD78.24, possible integral membrane protein, len: 293 aa, possible membrane spanning hydrophobic regions 3 3 19 10 [Reporter]SCO3271 (13K6)_PCR/5445 [Gene]SCO3271/4966 NC_003888 SCE39.21, possible dehydrogenase, len: 100aa; similar to TR:Q53783 (EMBL:L33871) vdh, valine dehydrogenase from Streptomyces ambofaciens (120 aa) fasta scores; opt: 92, z-score: 127.5, E(): 8.6, (29.9% identity in 107 aa overlap). 3 3 19 9 [Reporter]SCO0080 (14G6)_PCR/5444 [Gene]SCO0080/4965 NC_003888 SCJ11.09, unknown, len: 171 aa 3 3 19 8 [Reporter]SCO1810 (15C6)_PCR/5443 [Gene]SCO1810/4964 NC_003888 SCI28.04c, putative membrane protein, len: 68 aa. Contains possible hydrophobic membrane spanning regions 3 3 19 7 [Reporter]SCO2868 (16O2)_PCR/5442 [Gene]SCO2868/4963 NC_003888 SCE6.05, hypothetical protein, len: 159 aa. The C-terminus shares 54.1% identity in 74 aa overlap with Streptomyces coelicolor TR:CAB53304(EMBL:AL109973) hypothetical 10.9 KD protein, SCJ30.09C (96 aa); similar also to the SCP1 plasmid borne TR:Q9ACW6 (EMBL:AL590464) Streptomyces coelicolor conserved hypothetical protein SCP1.185, 184 aa; fasta scores: opt: 520 Z-score: 652.0 E(): 1.1e-28; 49.390% identity in 164 aa overlap 3 3 19 6 [Reporter]SCO1731 (17K2)_PCR/5441 [Gene]SCO1731/4962 NC_003888 SCI11.20c, hypothetical protein, len: 205 aa; weakly similar to parts of many hypothetical proteins, many of which resemble methyltransferases e.g. TR:Q48938 (EMBL:X93084) Methanosarcina barkeri ORF3 from molybdenum formylmethanofuran dehydrogenase cluster (262 aa), fasta scores; opt: 445 z-score: 518.9 E(): 1.4e-21, 37.8% identity in 193 aa overlap. Weak similarity to many others from S.coelicolor e.g. TR:Q9ZC03 (EMBL:AL033505) S.coelicolor hypothetical protein (273 aa) (35.2% identity in 142 aa overlap) 3 2 2 18 [Reporter]SCO6197 (4G23)_PCR/3063 [Gene]SCO6197/2789 NC_003888 SC2G5.18c, possible secreted protein, len: 180 aa; similar to TR:O87846 (EMBL:AL031013) putative secreted protein from Streptomyces coelicolor (168 aa) fasta scores; opt:476, z-score: 556.4, E(): 1e-23, (45.3% identity in 170 aa overlap). Contains possible N-terminal signal sequence 3 3 19 5 [Reporter]SCO1739 (18G2)_PCR/5440 [Gene]SCO1739/4961 NC_003888 SCI11.28c, probable DNA polymerase III, alpha chain, len: 1185 aa; similar to many e.g. SW:DP3A_ECOLI (EMBL:M19334), dnaE, Escherichia coli DNA polymerase III, alpha chain (1160 aa), fasta scores; opt: 1177 z-score: 1220.7 E(): 0, 28.4% identity in 1135 aa overlap 3 2 2 17 [Reporter]SCO7136 (5C23)_PCR/3062 [Gene]SCO7136/2788 NC_003888 SC4B10.37, conserved hypothetical protein, len: 247 aa; similar to TR:CAB76867 (EMBL:AL159139) Streptomyces coelicolor hypothetical 27.0 kD protein SCL6.02 , 255 aa; fasta scores: opt: 504 z-score: 571.2 E(): 2.4e-24; 41.3% identity in 247 aa overlap 3 3 19 4 [Reporter]SCO6294 (19C2)_PCR/5439 [Gene]SCO6294/4960 NC_003888 SCBAC8D1.07c, possible gntR-family regulatory protein, len: 263 aa; similar to TR:Q9ZBS9 (EMBL:AL034446) Streptomyces coelicolor putative gntR-family transcriptional regulator SC1A9.23, 273 aa; fasta scores: opt: 575 Z-score: 630.3 bits: 124.3 E(): 1.6e-27; 47.945% identity in 219 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 3 2 2 16 [Reporter]SCO7301 (6O19)_PCR/3061 [Gene]SCO7301/2787 NC_003888 SC5F8.11c, unknown, len: 248 aa. Highly similar to many beta-lactamase-like proteins including: Mycobacterium avium TR:Q9XC16(EMBL:AF152394) beta-lactamase-like protein, BllP (247 aa), fasta scores opt: 789 z-score: 885.8 E():0 48.1% identity in 241 aa overlap. Contains a Pfam match to entry PF00753 lactamase_B, Metallo-beta-lactamase superfamily. 3 2 2 15 [Reporter]SCO4412 (7K19)_PCR/3060 [Gene]SCO4412/2786 NC_003888 SC6F11.10, possible regulatory protein, len: 163 aa; similar to TR:Q9ZBT2 (EMBL:AL034446) Streptomyces coelicolor putative regulatory protein SC1A9.20, 135 aa; fasta scores: opt: 227 z-score: 286.6 E(): 1.8e-08; 43.0% identity in 135 aa overlap 3 2 2 14 [Reporter]SCO6875 (8G19)_PCR/3059 [Gene]SCO6875/2785 NC_003888 SC7F9.27c, unknown, len: 141 aa. 3 2 2 13 [Reporter]SCO7151 (9C19)_PCR/3058 [Gene]SCO7151/2784 NC_003888 SC9A4.13, conserved hypothetical protein, len: 267 aa; similar to TR:Q9S1U9 (EMBL:AL109950) Streptomyces coelicolor hypothetical 25.2 kDa protein, 242 aa; fasta scores: opt: 358 z-score: 430.9 E(): 1.6e-16; 32.6% identity in 258 aa overlap 3 2 2 12 [Reporter]SCO2340 (10O15)_PCR/3057 [Gene]SCO2340/2783 NC_003888 SCC53.31, hypothetical protein, len: 135 aa; similar to SW:P36677 (YHDN_ECOLI) hypothetical protein from Escherichia coli (122 aa) fasta scores; opt: 133, z-score: 164.8, E(): 0.1, 33.0% identity in 97 aa overlap. 3 2 2 10 [Reporter]SCO4254 (12G15)_PCR/3055 [Gene]SCO4254/2782 NC_003888 SCD8A.27, hypothetical protein, len: 956 aa; similar to C-terminal region of TR:CAB70627 (EMBL:AL137242) Streptomyces coelicolor large Ala/GLu-rich protein SC8F4.01c, 1326 aa; fasta scores: opt: 319 z-score: 268.7 E(): 1.6e-07; 25.1% identity in 798 aa overlap and to SW:TOLA_ECOLI (EMBL:M28232) Escherichia coli TolA protein, 421 aa; fasta scores: opt: 325 z-score: 280.1 E(): 3.6e-08; 38.4% identity in 255 aa overlap. Contains possible colied-coil region at aprox. residues 96..475. Rich in alanine and glutamic acid residues 3 2 2 9 [Reporter]SCO3565 (13C15)_PCR/3054 [Gene]SCO3565/2781 NC_003888 SCH5.28, possible integral membrane transport protein, len: 162aa; similar to TR:O69637 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (172 aa) fasta scores; opt: 265, z-score: 318.8, E(): 1.9e-10, (37.4% identity in 155 aa overlap). Also limited similarity to TR:O51582 (EMBL:AE001165) proposed sodium/proton antiporter from Borrelia burgdorferi (449 aa) fasta scores; opt: 104, z-score: 127.7, E(): 8.3, (32.5% identity in 77 aa overlap) and SW:XYLH_HAEIN proposed xylose transport permease from Haemophilus influenzae (375 aa) blast scores (3-109aa) Identities = 33/108 (30%), Positives = 53/108 (49%); (97-127aa) Identities = 9/31 (29%), Positives = 18/31 (58%). Contains possible membrane spanning hydrophobic regions. 3 2 2 8 [Reporter]SCO4861 (14O11)_PCR/3053 [Gene]SCO4861/2780 NC_003888 SCK20.02, unknown, len: 181 aa 3 3 19 3 [Reporter]SCP1.136 (19O22)_PCR/5438 [Gene]SCP1.136/4959 NC_003888 SCP1.136, possible helicase, len: 879aa; similar to other large proteins eg. TR:Q9L8V8 (EMBL:AF194023) helicase-like protein (881 aa) fasta scores; opt: 377, z-score: 397.0, E(): 1.2e-14, 37.2% identity in 916 aa overlap. Contains Pfam match to entry PF00271 helicase_C, Helicases conserved C-terminal domain. Note that this protein is inverted and repeated at complement(215655..217487) CDS SCP1.216Ac fasta scores; opt: 3788, z-score: 3530.0, E(): 0, 93.0% identity in 599 aa overlap. SCP1.216Ac is truncated at the N-terminus suggesting that either SCP1.216Ac is deleted or SCP1.136 has an incorrectly designated translational start site. 3 3 19 2 [Reporter]SCO5107 (20G22)_PCR/5437 [Gene]SCO5107/4958 NC_003888 SCBAC31E11.03c, shdA2, probable succinate dehydrogenase flavoprotein subunit, len: 653 aa; highly similar to TR:AAK44480 (EMBL:AE006934) Mycobacterium tuberculosis CDC1551 FAD flavoprotein oxidase, putative MT0262, 646 aa; fasta scores: opt: 2941 Z-score: 3164.4 bits: 595.8 E(): 1.2e-168; 70.416% identity in 649 aa overlap and to SW:DHSA_RICPR (EMBL:M88696) Rickettsia prowazekii succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) SdhA, 596 aa; fasta scores: opt: 643 Z-score: 693.1 bits: 138.4 E(): 5.4e-31; 35.218% identity; in 619 aa overlap. Contains Pfam matches to entries PF00890 FAD_binding_2, FAD binding domain and PF02910 succ_DH_flav_C, Fumarate reductase/succinate dehydrogenase flavoprotein C-terminal domain and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop 3 3 18 22 [Reporter]SCO1117 (1G18)_PCR/5435 [Gene]SCO1117/4957 NC_003888 2SCG38.10c, possible secreted protein, len: 345 aa; similar to TR:Q9RD79 (EMBL:AL136502) Streptomyces coelicolor hypothetical 41.5 kDa protein SCF43.09, 411 aa; fasta scores: opt: 813 z-score: 857.3 E(): 0; 44.1% identity in 358 aa overlap. Contains possible N-terminal region signal peptide sequence 3 3 18 21 [Reporter]SCO5470 (2C18)_PCR/5434 [Gene]SCO5470/4956 NC_003888 SC2A11.04c, glyA2, probable serine hydroxymethyltransferase, len: 420 aa; highly similar to many e.g. GLYA_ECOLI serine hydroxymethyltransferase (EC 2.1.2.1) (417 aa), fasta scores; opt: 1530 z-score: 1748.7 E(): 0, 56.1% identity in 412 aa overlap. Contains PS00096 Serine hydroxymethyltransferase pyridoxal-phosphate attachment site and Pfam match to entry PF00464 SHMT, Serine hydroxymethyltransferase, score 773.50, E-value 6.4e-232 3 3 18 20 [Reporter]SCO6810 (3O14)_PCR/5433 [Gene]SCO6810/4955 NC_003888 SC1A2.19c, unknown, len: 152 aa. Highly similar to proteins of undefined function from Mycobacterium tuberculosis SW:YQ11_MYCTU (EMBL:Z80225) (152 aa), fasta scores opt: 509 z-score: 630.7 E(): 1e-27 55.0% identity in 149 aa overlap and Bacillus subtilis SW:YQCK_BACSU (EMBL:D32216) (146 aa), fasta scores opt:349 z-score: 437.2 E():6e-17 34.9% identity in 152 aa overlap 3 3 18 19 [Reporter]SCO7208 (4K14)_PCR/5432 [Gene]SCO7208/4954 NC_003888 SC2H12.07c, unknown, len: 77 aa. 3 3 18 18 [Reporter]SCO2381 (5G14)_PCR/5431 [Gene]SCO2381/4953 NC_003888 SC4A7.09, hypothetical protein, len: 278 aa; simialr to various hypothetical proteins, e.g. TR:Q9X8M2 (EMBL:AL049628) Streptomyces coelicolor hypothetical 31.5 kD protein SCE94.16c, 280 aa; fasta scores: opt: 1333 z-score: 1595.1 E(): 0; 76.4% identity in 271 aa overlap 3 3 18 17 [Reporter]SCO7761 (6C14)_PCR/5430 [Gene]SCO7761/4952 NC_003888 SC5E9.09, unknown, doubtful CDS, len: 219aa; appears to lie within a region of degraded DNA. 3 2 2 7 [Reporter]SCO0721 (15K11)_PCR/3052 [Gene]SCO0721/2779 NC_003888 SCF42.31c, possible glycosyl transferase, len: 792 aa. Similar to Lactobacillus sanfrancisco TR:O87772 (EMBL:AJ224340) maltosephosphorylase (EC 2.4.1.8) MapA (753 aa), fasta scores opt: 1045 z-score: 1168.4 E():0 29.5% identity in 766 aa overlap. Also highly similar to a number of proteins of undefined function e.g. Mycobacterium tuberculosis SW:YY01_MYCTU(EMBL:Z77165) (786 aa), fasta scores opt: 3054 z-score: 3419.0 E():0 59.1% identity in 785 aa overlap 3 3 18 16 [Reporter]SCO6789 (7O10)_PCR/5429 [Gene]SCO6789/4951 NC_003888 SC6A5.38, possible fatty oxidation protein, len: 733aa; similar to many eg. SW:FADB_ECOLI FadB, a multifunctional protein from the Escherichia coli fadBA operon, encoding the fatty acid-oxidizing multienzyme complex (729 aa) fasta scores; opt: 1119, z-score: 1146.7, E(): 0, (32.3% identity in 691 aa overlap). Contains Pfam matches to entry PF00378 ECH, Enoyl-CoA hydratase/isomerase family and entry PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase and a Prosite match to PS00923 Aspartate and glutamate racemases signature 1. 3 2 2 6 [Reporter]SCO3134 (16G11)_PCR/3051 [Gene]SCO3134/2778 NC_003888 SCE66.13c, possible two-component system response regulator, len: 318 aa; similar to many proposed regulators e.g. TR:Q9RI42 (EMBL:AL109989) putative LuxR family two-component response regulator (233 aa) fasta scores; opt: 299, z-score: 339.4, E(): 2e-11, 31.6% identity in 234 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain, Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and helix-turn-helix motif (Score 1492 (+4.27 SD) in SCE66.13c at residue 253-274). 3 3 18 15 [Reporter]SCO6007 (8K10)_PCR/5428 [Gene]SCO6007/4950 NC_003888 SC7b7.04, probable integral membrane transport protein, len: 301 aa; similar to many members of the MALFG subfamily of the binding-protein-dependant transport systems membrane proteins eg. TR:Q06111 (EMBL:X66092) putative transport system permease protein from Clostridium perfringens (275 aa), fasta scores; opt: 590 z-score: 796.5 E(): 0, 33.6% identity in 265 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature 3 2 2 5 [Reporter]SCO3146 (17C11)_PCR/3050 [Gene]SCO3146/2777 NC_003888 SCE66.25c, possible secreted protein, len: 490 aa; similar to TR:Q9K3R9 (EMBL:AL359989) Streptomyces coelicolor putative membrane protein SCE66.24c, 497 aa; fasta scores: opt: 430 Z-score: 454.0 E(): 1.2e-17; 33.140% identity) in 516 aa overlap. Contains possible N-terminal signal sequence 3 2 2 4 [Reporter]SCO0401 (18O7)_PCR/3049 [Gene]SCO0401/2776 NC_003888 SCF62.27, possible aminotransferase, len: 441 aa. Similar to many including Pseudomonas aeruginosa SW:GSA_PSEAE (EMBL:X82072) glutamate-1-semialdehyde 2,1-aminomutase (EC 5.4.3.8) (glutamate-1-semialdehyde aminotransferase) (427 aa), fasta scores opt: 475 z-score: 540.8 E(): 9.5e-23 34.8% identity in 434 aa overlap and Streptomyces coelicolor TR:CAB39702 (EMBL: AL049485) probable aminotransferase SC6A5.18 (461 aa), fasta scores opt: 464 z-score: 527.9 E(): 4.9e-22 32.4% identity in 407 aa overlap. Contains 2x Pfam matches to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate 3 2 2 3 [Reporter]SCP1.232 (19K7)_PCR/3048 [Gene]SCP1.232/2775 NC_003888 SCP1.232, mmyE, possible enoyl reductase, len: 387aa; previously sequenced and annotated as TR:Q9JN79 (EMBL:AJ276673). Similar to SW:P77258 (NEMA_ECOLI) N-ethylmaleimide reductase (EC 1.-.-.-) from Escherichia coli (365 aa) fasta scores; opt: 507, z-score: 566.6, E(): 4.3e-24, 31.6% identity in 380 aa overlap. Contains Pfam match to entry PF00724 oxidored_FMN, NADH:flavin oxidoreductase / NADH oxidase family. 3 2 2 2 [Reporter]SCP1.226 (20C7)_PCR/3047 [Gene]SCP1.226/2774 NC_003888 SCP1.226, unknown, len: 128aa; previously sequenced and annotated as TR:Q9JN72 (EMBL:AJ276673). Similar to TR:Q9KXJ3 (EMBL:AL357591) hypothetical protein from Streptomyces coelicolor (128 aa) fasta scores; opt: 406, z-score: 539.2, E(): 1.5e-22, 52.3% identity in 128 aa overlap. 3 2 1 22 [Reporter]SCO5195 (1C3)_PCR/3045 [Gene]SCO5195/2773 NC_003888 2SC3B6.19, hypothetical protein, len: 584 aa; N-terminal region similar to N-terminal region of TR:Q9RSA4 (EMBL:AE002055) Deinococcus radiodurans tellurium resistance protein TerA DR2223, 400 aa; fasta scores: opt: 552 z-score: 357.3 E(): 1.9e-12; 40.6% identity in 239 aa overlap and C-terminal region similar to TR:Q9RWP1 (EMBL:AE001920) Deinococcus radiodurans conserved hypothetical protein DR0625, 254 aa; blastp scores: Score = 262 (92.2 bits), Expect = 7.4e-22, P = 7.4e-22, Identities = 68/208 (32%), Positives = 117/208 (56%) 3 2 1 21 [Reporter]SCO4925 (1O23)_PCR/3044 [Gene]SCO4925/2772 NC_003888 SCK13.17c, unknown, len: 69 aa 3 2 1 20 [Reporter]SCO4562 (2K23)_PCR/3043 [Gene]SCO4562/2771 NC_003888 SCD16A.21c, nuoA, NADH dehydrogenase subunit, len: 119 aa; similar to many e.g. TR:P95181 (EMBL:Z83867) NuoA, NADH dehydrogenase subunit from Mycobacterium tuberculosis (128 aa) fasta scores; opt: 405, z-score: 534.2, E(): 2e-22, (68.8% identity in 128 aa overlap). Contains Pfam match to entry PF00507 oxidored_q4, NADH-ubiquinone/plastoquinone oxidoreductase, chain 3. Contains possible hydrophobic membrane spanning regions 3 2 1 19 [Reporter]SCO7404 (3G23)_PCR/3042 [Gene]SCO7404/2770 NC_003888 SC10G8.32c, unknown, len: 263 aa. Highly similar to a number of proteins of undefined function from Streptomyces coelicolor including: TR:O86541 (EMBL:AL031350) SC1F3.04 (261 aa), fasta scores opt: 1155 z-score: 1354.8 E():0 67.9% identity in 262 aa overlap and TR:O86718 (EMBL:AL031515) SC5C7.21C (271 aa), fasta scores opt: 894 z-score: 1050.3 E(): 0 54.0% identity in 272 aa overlap. 3 3 18 14 [Reporter]SCO5508 (9G10)_PCR/5427 [Gene]SCO5508/4949 NC_003888 SC8D9.20c, possible oxidoreductase, len: 447aa; similar to many of undefined function and SW:DHGB_ACICA glucose dehydrogenase-B from Acinetobacter calcoaceticus (478 aa) fasta scores; opt: 247, z-score: 253.8, E(): 7.9e-07, (27.4% identity in 369 aa overlap). 3 3 18 13 [Reporter]SCO7369 (10C10)_PCR/5426 [Gene]SCO7369/4948 NC_003888 SC9H11.23c, possible transmembrane transport protein, len: 406 aa. Similar to many putative membrane transport proteins e.g. Zymomonas mobilis TR:Q9XBR9(EMBL:AF157493) putative transporter, DitE (431 aa), fasta scores opt: 499 z-score: 512.6 E(): 4.3e-21 26.3% identity in 407 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 3 3 18 12 [Reporter]SCO4382 (11O6)_PCR/5425 [Gene]SCO4382/4947 NC_003888 SCD10.14, probable acyl CoA dehydrogenase, len: 382 aa; similar to SW:ACDB_MYCTU (EMBL:Z79700) Mycobacterium tuberculosis probable acyl CoA dehydrogenase MTCY10D7.02, 388 aa; fasta scores: opt: 1694 z-score: 1964.7 E(): 0; 64.3% identity in 387 aa overlap. Contains 2 Pfam matches to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase 3 3 18 11 [Reporter]SCO3953 (12K6)_PCR/5424 [Gene]SCO3953/4946 NC_003888 SCD78.20c, possible phosphotransferase, len: 204 aa; similar to conserved hypotheticals including YJII_ECOLI from Escherichia coli (218 aa) fasta scores;opt: 506, z-score: 785.8, E(): 0, (44.1% identity in 195 aa overlap). Also similar to predicted phosphotransferase TR:O14045 (EMBL:Z99259) from Schizosaccharomyces pombe (361 aa) fasta scores; opt: 268, z-score: 242.6, E(): 2.9e-06, (36.0%identity in 175 aa overlap) 3 3 18 10 [Reporter]SCO3076 (13G6)_PCR/5423 [Gene]SCO3076/4945 NC_003888 SCE25.17, possible integral membrane protein, len: 156 aa. Contains possible hydrophobic membrane spanning regions 3 3 18 9 [Reporter]SCO2895 (14C6)_PCR/5422 [Gene]SCO2895/4944 NC_003888 SCE6.32c possible integral membrane transport protein, len: 295 aa. Similar to Deinococcus radiodurans TR:AAF09789(EMBL:AE001882) conserved hypothetical protein (277 aa), fasta scores. Also weakly similar to the adjoining CDS SCE6.33c, fasta scores opt: 174 z-score: 186.6 E(): 6.9e-05 29.4% identity in 269 aa overlap. Contains a Prosite hit to PS00216 Sugar transport proteins signature 1 and multiple possible membrane spanning hydrophobic domains. Also contains an N-terminal 5xEAA repeat sequence. 3 3 18 8 [Reporter]SCO0516 (15O2)_PCR/5421 [Gene]SCO0516/4943 NC_003888 SCF6.12, possible lipoprotein, len:392 aa. Weakly similar to Methylobacterium extorquens TR:O30796 (EMBL; AF017434) hypothetical protein MxaE (281 aa), fasta scores opt: 205 z-score: 214.7 E(): 0.00014 32.5% identity in 295 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 3 3 18 7 [Reporter]SCO1735 (16K2)_PCR/5420 [Gene]SCO1735/4942 NC_003888 SCI11.24c, probable secreted lipase, len: 290 aa; similar to many lipases e.g. TR:O33969 (EMBL:U80063), lipA, Streptomyces cinnamoneus lipase (275 aa), fasta scores; opt: 575 z-score: 653.1 E(): 4.8e-29, 47.6% identity in 254 aa overlap. Contains probable N-terminal signal sequence 3 2 1 18 [Reporter]SCO6193 (4C23)_PCR/3041 [Gene]SCO6193/2769 NC_003888 SC2G5.14c, possible transcriptional regulator, len: 943 aa; similar to TR:O05478 (EMBL:M86351) LipA transcriptional activator from Streptomyces sp. M11 (934aa) fasta scores; opt: 387, z-score: 377.7, E(): 9.5e-14,(31.1% identity in 875 aa overlap). Contains PS00622 Bacterial regulatory proteins, luxR family signature and PS00017 ATP /GTP-binding site motif A (P-loop). Also contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score53.30, E-value 5.3e-12. 3 3 18 6 [Reporter]SCO0455 (17G2)_PCR/5419 [Gene]SCO0455/4941 NC_003888 SCF51A.33, putative binding-protein dependent transport protein, len: 274 aa. Similar to many e.g. Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) TR:O30833 (EMBL; AF018073) sorbitol/mannitol transport inner membrane protein (276 aa), fasta scores opt: 452 z-score: 556.9 E(): 1.2e-23 32.0% identity in 272 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Contains multiple possible membrane spanning hydrophobic domains. 3 2 1 17 [Reporter]SCO7037 (5O19)_PCR/3040 [Gene]SCO7037/2768 NC_003888 SC4G1.03, possible secreted protein, len: 1131 aa; similar to TR:CAB88148 (EMBL:AL352956) Streptomyces coelicolor putative transmembrane protein SC1H10.04c, 1433 aa; fasta scores: opt: 1569 z-score: 1600.6 E(): 0; 36.4% identity in 1265 aa overlap. Contains possible cleavable N-terminal signal peptide sequence 3 3 18 4 [Reporter]SCO6113 (18O22)_PCR/5417 [Gene]SCO6113/4940 NC_003888 SCBAC33E8.01c, possible peptide ABC transporter integral membrane protein (fragment), len: >47 aa. Similar to the N-terminal part of TR:Q9KGM9 (EMBL:AP001507) Bacillus halodurans oligopeptide ABC transporter (permease) BH0029, 314 aa; fasta scores: opt: 136 Z-score: 200.4 bits: 42.5 E(): 0.0014; 47.826% identity in 46 aa overlap,SCBAC1A6.37c, probable oligopeptide ABC transporter integral membrane protein (fragment), len: >312 aa; similar to SW:APPB_BACSU (EMBL:U20909) Bacillus subtilis oligopeptide transport system permease protein AppB, 317 aa; fasta scores: opt: 763 z-score: 895.6 E(): 0; 37.0% identity in 311 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 3 2 1 16 [Reporter]SCO7297 (6K19)_PCR/3039 [Gene]SCO7297/2767 NC_003888 SC5F8.07c, possible two-component sensor histidine kinase, len: 492 aa. The C-terminus of this protein is similar to many sensor histidine protein kinases e.g. Salmonella typhimurium SW:NTRB_SALTY (EMBL:X85104) nitrogen regulation protein NR(II) (EC 2.7.3.-) (349 aa), fasta scores opt: 313 z-score: 363.5 E(): 8.9e-13 27.9% identity in 297 aa overlap. Contains a Pfam match to entry PF00512 signal, Histidine kinase. Also contains a 4xTPD repeat in the C-terminus. 3 2 1 15 [Reporter]SCO7406 (7G19)_PCR/3038 [Gene]SCO7406/2766 NC_003888 SC6D11.02C, possible secreted protein, len: 691 aa. Contains a 8xGT repeat region within the C-terminus and a possible N-terminal signal sequence. 3 2 1 14 [Reporter]SCO6871 (8C19)_PCR/3037 [Gene]SCO6871/2765 NC_003888 SC7F9.23c, possible lipoprotein, len: 355 aa. Weakly similar to Streptomyces coelicolor TR:CAB61724(EMBL:AL133220) hypothetical 31.7 KD protein, SCC75A.24 (295 aa), fasta scores opt: 316 z-score: 356.8 E(): 1.8e-12 27.3% identity in 297 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 3 2 1 13 [Reporter]SCO6118 (9O15)_PCR/3036 [Gene]SCO6118/2764 NC_003888 SC9B2.05, possible AraC family transcriptional regulator, len: 324aa; similar to many eg. TR:O31249 (EMBL:AJ002316) AlkR, transcriptional regulator of alkane utilization in Acinetobacter sp. strain ADP1 (306 aa) fasta scores; opt: 390, z-score: 436.9, E(): 4.8e-17, (28.3% identity in 314 aa overlap). Contains PS00041 Bacterial regulatory proteins, araC family signature and Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. Also contains a probable helix-turn-helix (+3.45 SD) at residues 238 to 259. 3 2 1 12 [Reporter]SCO2336 (10K15)_PCR/3035 [Gene]SCO2336/2763 NC_003888 SCC53.27, possible integral membrane transport protein, len: 498aa; similar to many eg. SW:Q47421 (OUSA_ERWCH) osmoprotectant uptake protein from Erwinia chrysanthemi (498 aa) fasta scores; opt: 1554, z-score: 1636.5, E(): 0, 50.3% identity in 445 aa overlap and TR:CAB76296 (EMBL:AL158057) putative membrane transport protein from Streptomyces coelicolor (521 aa) fasta scores; opt: 1703, z-score: 1792.6, E(): 0, 57.2% identity in 437 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and Prosite match to PS00217 Sugar transport proteins signature 2. 3 2 1 11 [Reporter]SCO4112 (11G15)_PCR/3034 [Gene]SCO4112/2762 NC_003888 SCD17A.04, unknown, len: 136 aa 3 2 1 9 [Reporter]SCO3449 (13O11)_PCR/3032 [Gene]SCO3449/2761 NC_003888 SCE46.06c, unknown, len: 106 aa 3 2 1 8 [Reporter]SCO3685 (14K11)_PCR/3031 [Gene]SCO3685/2760 NC_003888 SCH35.39c, unknown, len: 133aa; similar to TR:Q9X8W6 (EMBL:AL078610) SCH35.40 hypothetical protein (196 aa) fasta scores; 47.794% identity (50.000% ungapped) in 136 aa overlap . 3 3 18 3 [Reporter]SCO1304 (19K22)_PCR/5416 [Gene]SCO1304/4939 NC_003888 SCBAC36F5.15, conserved hypothetical protein, len: 135 aa; similar to TR:BAB34472 (EMBL:AP002554) Escherichia coli O157:H7 hypothetical 14.7 kDa protein ECS1049, 137 aa; fasta scores: opt: 401 Z-score: 483.6 bits: 95.2 E(): 2.5e-19; 50.376% identity in 133 aa overlap. Contains Pfam match to entry PF02629 DUF184, Uncharacterized ACR, COG1832 3 3 18 2 [Reporter]SCO2823 (20C22)_PCR/5415 [Gene]SCO2823/4938 NC_003888 SCBAC17F8.14c, possible decarboxylase, len: 300 aa: similar to many e.g. SW:Q05115 (AMDA_BORBR) arylmalonate decarboxylase from Alcaligenes bronchisepticus (240 aa) fasta scores; opt: 328, Z-score: 331.2, 33.810% identity (35.323% ungapped) in 210 aa overlap and TR:Q54412 (EMBL:X52071) hypothetical protein which close alongside transfer RNA-gly in Streptomyces lividans (195 aa) fasta scores; opt: 295, Z-score: 300.7, 36.872% identity (38.824% ungapped) in 179 aa overlap. Also similar to neighbouring CDS SCBAC17F8.13c. 3 3 17 22 [Reporter]SCO1112 (1C18)_PCR/5413 [Gene]SCO1112/4937 NC_003888 2SCG38.05, probable oxidoreductase, len: 567 aa; similar to SW:AIDB_ECOLI (EMBL:L20915) Escherichia coli putative acyl-CoA dehydrogenase; adaptive response AidB protein, 541 aa; fasta scores: opt: 1437 z-score: 1553.6 E(): 0; 45.8% identity in 517 aa overlap. Contains 2x Pfam matches to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase and match to Prosite entry PS00073 Acyl-CoA dehydrogenases signature 2 3 3 17 21 [Reporter]SCO2562 (2O14)_PCR/5412 [Gene]SCO2562/4936 NC_003888 SCC77.29c, lepA, GTP-binding protein, len: 622 aa. Highly similar to many GTP-binding proteins including: Escherichia coli SW:LEPA_ECOLI (EMBL:K00426) GTP-binding protein LepA (599 aa), fasta scores opt: 2156 z-score: 2321.8 E():0 54.1% identity in 592 aa overlap and Mycobacterium tuberculosis SW:LEPA_MYCTU(EMBL:Z81368) GTP-binding protein LepA (653 aa), fasta scores opt: 3045 z-score: 3277.7 E():0 74.6% identity in 623 aa overlap. Contains Prosite hits to PS00301 GTP-binding elongation factors signature and PS00017 ATP/GTP-binding site motif A (P-loop). Also contains Pfam match to entry PF00009 GTP_EFTU. 3 3 17 20 [Reporter]SCO6806 (3K14)_PCR/5411 [Gene]SCO6806/4935 NC_003888 SC1A2.15c, possible phage integrase, len: 405 aa. Weakly similar to Corynephage 304L TR:Q9ZWV7 (EMBL:Y18058) integrase (395 aa), fasta scores opt: 190 z-score: 224.2 E(): 4.4e-05 24.6% identity in 391 aa overlap and Streptomyces coelicolor TR:Q9Z4Y1 (EMBL:AL035707) putative integrase, SCE29.19C (384 aa), fasta scores opt: 191 z-score: 225.6 E(): 3.7e-05 27.4% identity in 358 aa overlap. Contains a Pfam match to entry PF00589 Phage_integrase, Phage integrase family. 3 3 17 19 [Reporter]SCO5487 (4G14)_PCR/5410 [Gene]SCO5487/4934 NC_003888 SC2A11.21c, unknown, len: 242 aa; some similarity in C-terminus to enterobacterial ampD genes e.g. AMPD_ECOLI AMPD protein (183 aa), fasta scores; opt: 210 z-score: 212.6 E(): 0.00014, 31.5% identity in 149 aa overlapand Enterobacter cloacae TR:Q46957 (EMBL:U40785) anhydro-n-acetylmuramyl-tripeptide amidase (187 aa), fasta scores; opt: 207 z-score: 192.0 E(): 0.0019, 31.6% identity in 155 aa overlap 3 3 17 18 [Reporter]SCO2377 (5C14)_PCR/5409 [Gene]SCO2377/4933 NC_003888 SC4A7.05c, probable aldo/keto reductase, len: 338 aa; similar to TR:Q9X5G7 (EMBL:AF124928) Streptomyces clavuligerus putative aldo/keto reductase family 2 enzyme Cvm1, 344 aa; fasta scores: opt: 916 z-score: 1035.7 E(): 0; 47.7% identity in 331 aa overlap 3 3 17 17 [Reporter]SCO6524 (6O10)_PCR/5408 [Gene]SCO6524/4932 NC_003888 SC5C7.09c, probable integral membrane protein, len: 208 aa. Contains possible hydrophobic membrane spanning regions 3 2 1 7 [Reporter]SCO3346 (15G11)_PCR/3030 [Gene]SCO3346/2759 NC_003888 SCE7.13c, possible transcriptional regulator, len: 204 aa; similar to SW:UIDR_ECOLI uid operon repressor from Escherichia coli (196 aa) fasta scores; opt: 168, z-score: 207.0, E(): 0.00033, (28.7% identity in 188 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Note N-terminal region previously sequenced and annotated as a UDP-MurNAc L-alanine ligase-like protein therefore almost (conflict of two amino acids) identical to TR:O87451 (EMBL:AF068843) (63 aa) fasta scores; opt: 392, z-score: 473.9, E(): 4.5e-19, (96.8% identity in 63 aa overlap). 3 3 17 16 [Reporter]SCO6784 (7K10)_PCR/5407 [Gene]SCO6784/4931 NC_003888 SC6A5.33c, possible regulatory protein, len: 223 aa; similar to hypothetical proteins e.g. TR:P96839 (EMBL:Z92774) from Mycobacterium tuberculosis (200 aa) fasta scores; opt: 262, z-score: 321.3, E(): 1.4e-10, (29.1% identity in 182 aa overlap). Also short regions of similarity to regulatory proteins e.g. SW:TER8_PASMU tetracycline repressor protein from Pasteurella multocida (207 aa) fasta scores; opt: 152, z-score: 190.7, E(): 0.0025, (41.4% identity in 58 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and probable helix-turn-helix (+2.65 SD) 44-6 5aa 3 2 1 6 [Reporter]SCO0329 (16C11)_PCR/3029 [Gene]SCO0329/2758 NC_003888 SCF12.08, possible esterase len: 376 aa; similar to many e.g. TR:O87861 (EMBL:Z15137) esterase A from Streptomyces chrysomallus (389 aa) fasta scores; opt: 503, z-score: 581.9, E(): 4.6e-25, 34.9% identity in 378 aa overlap 3 3 17 15 [Reporter]SCO5307 (8G10)_PCR/5406 [Gene]SCO5307/4930 NC_003888 SC6G9.26c, hypothetical protein, len: 274 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Alternative start codons are present at codons 8 and 14 3 2 1 5 [Reporter]SCO3894 (17O7)_PCR/3028 [Gene]SCO3894/2757 NC_003888 SCH24.16c, probable transmembrane protein, len: 811 aa; unknown function, similar to hypothetical proteins e.g. the N-terminal half of TR:O05435 (EMBL:Z94121) Mycobacterium tuberculosis hypothetical protein (1184 aa), fasta scores; opt: 573 z-score: 440.2 E(): 3.4e-17, 31.1% identity in 573 aa overlap and TR:Q50198 (EMBL:L39923) Mycobacterium leprae hypothetical protein (379 aa) (31.7% identity in 398 aa overlap). Also weakly similar to SW:MVIN_SALTY (EMBL:Z26133) Salmonella typhimurium 'mvin' (524 aa) (26.8% identity in 456 aa overlap). The N-terminal third is hydrophilic, while the remainder contains hydrophobic, probable membrane-spanning regions 3 2 1 3 [Reporter]SCO7525 (19G7)_PCR/3026 [Gene]SCO7525/2756 NC_003888 SC8G12.01, possible oxidoreductase, partial CDS, len: >169 aa. Highly similar to several including: Streptomyces coelicolor TR:CAB52060(EMBL:AL109732) putative formate dehydrogenase, SC7H2.18 (759 aa), fasta scores opt:670 z-score: 812.0 E(): 0 62.3% identity in 167 aa overlap and Mycobacterium tuberculosis TR:YX31_MYCTU(EMBL:Z74024) hypothetical 84.6 kd protein (779 aa), fasta scores opt: 571 z-score: 692.2 E(): 4.3e-31 55.2% identity in 172 aa overlap.,SCBAC25F8.17, probable oxidoreductase (fragment), len: >629 aa; similar to N-terminal reguion of TR:AAK47294 (EMBL:AE007119) Mycobacterium tuberculosis CDC1551 formate dehydrogenase, alpha subunit, putative MT2968, 779 aa; fasta scores: opt: 2423 Z-score: 2763.1 bits: 521.7 E(): 2.7e-146; 56.640% identity in 625 aa overlap and of TR:CAB52060 (EMBL:AL109732) Streptomyces coelicolor putative formate dehydrogenase SC7H2.18, 759 aa; fasta scores: opt: 2617 Z-score: 2803.0 bits: 529.1 E(): 2.1e-150; 62.798% identity in 629 aa overlap. Contains 2x Pfam matches to entry PF00384 molybdopterin, Molybdopterin oxidoreductases and match to Prosite entry PS00697 ATP-dependent DNA ligase AMP-binding site and PS00402 Binding-protein-dependent transport systems inner membrane comp sign 2 2 23 22 [Reporter]SCO4933 (1J23)_PCR/3024 [Gene]SCO4933/2755 NC_003888 SCK13.25, unknown, len: 237 aa 2 2 23 21 [Reporter]SCO5820 (2F23)_PCR/3023 [Gene]SCO5820/2754 NC_003888 SC5B8.10, hrdB, major vegetative sigma factor, len: 511 aa; almost identical to, but 70 aa longer than, HRDB_STRCO P18183 Streptomyces coelicolor (442 aa), 99.8% identity in 442 aa overlap. A more upstream start site has been chosen due to extended homology with HrdB sigma factors from other Streptomyces species. Contains PS00715 and PS00716 Sigma-70 factors family signature 1 and 2 and Pfam match to entry sigma70 PF00140, Sigma-70 factors, score 477.38. Also contains 7 repeats of the sequence AKK(A/T)(A/T) and probable helix-turn-helix motifs from aa 471-492 (Score 1644, +4.7 9 SD) and aa 378-399 (Score 1108, +2.96 SD) 2 2 23 20 [Reporter]SCO7398 (3B23)_PCR/3022 [Gene]SCO7398/2753 NC_003888 SC10G8.26c, possible membrane transport protein, len: 694 aa. Highly similar to many members of the FecCD family of binding protein-dependent transport proteins e.g. Escherichia coli SW:FHUB_ECOLI(EMBL:X04319) ferrichrome transport protein FhuB precursor (660 aa), fasta scores opt: 510 z-score:0.0 E(): 0.0 33.93% identity in 672 aa overlap. Contains 2xPfam match to entry PF01032 FecCD_family, FecCD transport family and multiple possible membrane spanning hydrophobic domains. 2 2 23 19 [Reporter]SCO7620 (4N19)_PCR/3021 [Gene]SCO7620/2752 NC_003888 SC2H2.18, hypothetical protein, len: 119 aa; similar to TR:Q9K4K9 (EMBL:AL357613) Streptomyces coelicolor hypothetical 13.1 kDa protein SC5F8.15c, 117 aa; fasta scores: opt: 382 z-score: 486.2 E(): 1.5e-19; 53.0% identity in 117 aa overlap 2 2 23 18 [Reporter]SCO6712 (5J19)_PCR/3020 [Gene]SCO6712/2751 NC_003888 SC4C6.22, possible copper oxidase, len: 343 aa; weakly similar to members of the multicopper oxidase family e.g. to the N-terminus of SW:COPA_PSESM (EMBL:M19930), CopA, Pseudomonas syringae copper resistance protein A precursor (609 aa), fasta scores; opt: 170 z-score: 188.4 E(): 0.0036, 27.3% identity in 205 aa overlap. Also weakly similar to the blood coagulation factors which are structurally related to the multicopper oxidases. Similar to the N-terminus of TR:O53858 (EMBL:AL022004) Mycobacterium tuberculosis hypothetical protein (504 aa) (25.1% identity in 323 aa overlap). Contains PS00079 Multicopper oxidases signature 1 and PS00080 Multicopper oxidases signature 2 (the latter suggesting copper-binding) 2 2 23 17 [Reporter]SCO7564 (6F19)_PCR/3019 [Gene]SCO7564/2750 NC_003888 SC5F1.18, possible oligopeptide ABC transporter integral membrane protein, len: 330 aa; similar to TR:Q9KFX2 (EMBL:AP001508) Bacillus halodurans oligopeptide ABC transporter (permease) AppB, 305 aa; fasta scores: opt: 883 z-score: 1023.7 E(): 0; 44.8% identity in 299 aa overlap and to SW:OPPB_ECOLI (EMBL:AE000223) Escherichia coli oligopeptide transport system permease protein OppB, 306 aa; fasta scores: opt: 680 z-score: 790.0 E(): 0; 36.0% identity in 308 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 3 3 17 14 [Reporter]SCO5503 (9C10)_PCR/5405 [Gene]SCO5503/4929 NC_003888 SC8D9.15, possible acetyltransferase, len: 194aa; similar to many eg. TR:P96579 (EMBL:AB001488) probable acetyltransferase from Bacillus subtilis (183 aa) fasta scores; opt: 250, z-score: 327.1, E(): 6.5e-11, (27.9% identity in 172 aa overlap) 3 3 17 13 [Reporter]SCO2502 (10O6)_PCR/5404 [Gene]SCO2502/4928 NC_003888 SCC121.05, probable transport protein, len: 477 aa; similar to SW:AC22_STRCO (EMBL:M64683) Streptomyces coelicolor probable actinorhodin transporter ActII-2, 578 aa; fasta scores: opt: 902 z-score: 985.8 E(): 0; 36.6% identity in 437 aa overlap and to SW:PUR8_STRLP (EMBL:X76855) Streptomyces lipmanii (Streptomyces alboniger) Puromycin resistance protein Pur8, 503 aa; fasta scores: opt: 700 z-score: 766.7 E(): 0; 34.1% identity in 440 aa overlap 3 3 17 12 [Reporter]SCO4378 (11K6)_PCR/5403 [Gene]SCO4378/4927 NC_003888 SCD10.10, conserved hypothetical protein, len: 276 aa; similar to SW:YO36_MYCTU (EMBL:Z80233) Mycobacterium tuberculosis hypothetical 27.6 kD protein CY10H4.36c, 257 aa; fasta scores: opt: 1065 z-score: 1157.0 E(): 0; 61.5% identity in 257 aa overlap 3 3 17 10 [Reporter]SCO3071 (13C6)_PCR/5401 [Gene]SCO3071/4926 NC_003888 SCE25.12c, possible chlorohydrolase, len: 449 aa; similar to SW:ATZA_PSESD (EMBL:U55933) Pseudomonas sp. atrazine chlorohydrolase (EC 3.8.1.-) AtzA, 473 aa; fasta scores: opt: 401 z-score: 468.3 E(): 1.3e-18; 26.9% identity in 428 aa overlap. Contains Pfam match to entry PF01685 ATZ_TRZ, Chlorohydrolase 3 3 17 9 [Reporter]SCO5038 (14O2)_PCR/5400 [Gene]SCO5038/4925 NC_003888 SCK7.11c, possible integral membrane protein, len: 312 aa; similar to TR:Q9RDB7 (EMBL:AL136058) Streptomyces coelicolor putative membrane protein SCE20.08c, 383 aa; fasta scores: opt: 467 z-score: 517.9 E(): 2.2e-21; 31.2% identity in 301 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 17 8 [Reporter]SCO1884 (15K2)_PCR/5399 [Gene]SCO1884/4924 NC_003888 SCI7.02c, unknown, len: 281aa; 3 3 17 7 [Reporter]SCO1782 (16G2)_PCR/5398 [Gene]SCO1782/4923 NC_003888 SCI51.22c, possible membrane protein, len: 271 aa; unknown function, similar to hemolysins and hemolysin-like hypothetical proteins e.g. SW:HLYA_TREHY (EMBL:X61684), tlyA, Treponema hyodysenteriae hemolysin A (240 aa), fasta scores; opt: 457 z-score: 532.9 E(): 2.5e-22, 35.4% identity in 240 aa overlap and TR:Q50760 (EMBL:X98295), tlyA, Mycobacterium tuberculosis. Contains a hydrophobic, possible membrane-spanning region. Contains Pfam match to entry PF01479 S4, S4 domain and PS00017 ATP/GTP-binding site motif A (P-loop) 3 3 17 6 [Reporter]SCO0860 (17C2)_PCR/5397 [Gene]SCO0860/4922 NC_003888 SCM2.13c, probable cation-transporting ATPase, len: 762 aa. Highly similar to many other cation transporters e.g. Enterococcus hirae SW:COPA_ENTHR(EMBL:L13292) copper/potassium-transporting ATPase A (EC 3.6.1.36) (727 aa), fasta scores opt: 1287 z-score: 1275.2 E():0 37.8% identity in 749 aa overlap. Contains Prosite hits to PS01047 Heavy-metal-associated domain and PS00154 E1-E2 ATPases phosphorylation site. Also contains Pfam matches to entries PF00122 E1-E2_ATPase, E1-E2 ATPase and PF00403 HMA, Heavy-metal-associated domain as well as multiple possible membrane spanning hydrophobic domains. 2 2 23 16 [Reporter]SCO2677 (7B19)_PCR/3018 [Gene]SCO2677/2749 NC_003888 SC6D10.20c, probable ABC transpoter ATP-binding protein, len: 547 aa; similar to various ABC transpoter ATP-binding proteins, e.g. TR:O53204 (EMBL:AL021246) Mycobacterium tuberculosis ABC-transpoter ATP binding protein MTV008.33c, 558 aa; fasta scores: opt: 2937 z-score: 3148.6 E(): 0; 79.5% identity in 551 aa overlap and to SW:UUP_ECOLI (EMBL:AE000197) Echerichia coli ABC transporter ATP-binding protein Uup, 635 aa; fasta scores: opt: 1134 z-score: 1216.4 E(): 0; 36.6% identity in 519 aa overlap. Contains two Pfam matches to entry PF00005 ABC_tran, ABC transporter, two matches to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and two matches to Prosite entry PS00211 ABC transporters family signature. Contains also possible colied-coil region 3 3 17 5 [Reporter]SCO0623 (17O22)_PCR/5396 [Gene]SCO0623/4921 NC_003888 SCF56.07, probable membrane transport protein, len: 539 aa; similar to TR:Q9X8M3 (EMBL:AL049628) Streptomyces coelicolor putative exporter SCE94.17c, 531 aa; fasta scores: opt: 1316 z-score: 1386.9 E(): 0; 40.1% identity in 534 aa overlap and to SW:PUR8_STRLP (EMBL:X76855) Streptomyces lipmanii (Streptomyces alboniger) puromycin resistance protein Pur8, 503 aa; fasta scores: opt: 701 z-score: 741.2 E(): 0; 32.5% identity in 434 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter 2 2 23 15 [Reporter]SCO2003 (8N15)_PCR/3017 [Gene]SCO2003/2748 NC_003888 SC7H2.17c, polA, DNA polymerase I, len: 907 aa; similar to many eg. SW:DPO1_MYCTU polA, DNA polymerase I from Mycobacterium tuberculosis (904 aa) fasta scores; opt: 3296, z-score: 3592.4, E(): 0, (57.5% identity in 909 aa overlap). Contains Pfam match to entry PF00476 DNA_pol_A, DNA polymerase family A and to entry PF01367 5_3_exonuclease, 5'-3' exonuclease. 3 3 17 4 [Reporter]SCP1.134 (18K22)_PCR/5395 [Gene]SCP1.134/4920 NC_003888 SCP1.134, unknown, len: 108aa; 2 2 23 14 [Reporter]SCO5858 (9J15)_PCR/3016 [Gene]SCO5858/2747 NC_003888 SC9B10.25c, unknown, probable integral membrane protein, len: 393 aa; similar to TR:Q9I1K4 (EMBL:AE004652) Pseudomonas aeruginosa hypothetical protein PA2269, 401 aa; fasta scores: opt: 1176 Z-score: 1266.8 E(): 6.3e-63; 50.678% identity in 369 aa overlap 2 2 23 13 [Reporter]SCO2435 (10F15)_PCR/3015 [Gene]SCO2435/2746 NC_003888 SCC24.06, unknown, len: 175 aa; similar to TR:Q9F1V4 (EMBL:AB041825) Streptomyces ambofaciens ORF1 (genes for calcium binding) hypothetical protein, 131 aa; fasta scores: opt: 452 Z-score: 555.1 E(): 2.8e-23; 64.655% identity in 116 aa overlap 2 2 23 12 [Reporter]SCO4107 (11B15)_PCR/3014 [Gene]SCO4107/2745 NC_003888 SCD17.11, possible integral membrane protein, len: 454 aa. Similar to many e.g. Streptomyces coelicolor TR:O69922 (EMBL; AL023861) putative integral membrane protein SC3C8.09 (367 aa), fasta scores opt: 687 z-score: 689.9 E(): 4.5e-31 37.2% identity in 368 aa overlap. Contains possible membrane spanning hydrophobic domains. Contains probable coiled coil between residues 270..307 (probability 0.85). 2 2 23 11 [Reporter]SCO4161 (12N11)_PCR/3013 [Gene]SCO4161/2744 NC_003888 SCD84.28, possible molybdopterin converting factor, len: 84 aa; similar to TR:O53880 (EMBL:AL022004) Mycobacterium tuberculosis putative molybdopterin converting factor (subunit 1) MoaD-2, 92 aa; fasta scores: opt: 191 z-score: 269.0 E(): 1.6e-07; 45.9% identity in 85 aa overlap 2 2 23 10 [Reporter]SCO3444 (13J11)_PCR/3012 [Gene]SCO3444/2743 NC_003888 SCE46.01c, hypothetical protein (partial CDS), len: >38 aa ;similar to TR:CAB52841 (EMBL:AL109848) Streptomyces coelicolor hypothetical 68.4 kD protein; fasta scores: opt: 152 z-score: 252.5 E(): 1e-06; 52.8% identity in 36 aa overlap,SCE36.11c, partial CDS, conserved hypothetical protein, len: 612aa; similar to TR:P71741 (EMBL:Z81368) hypothetical protein from Mycobacterium tuberculosis (642 aa) fasta scores; opt: 1283, z-score: 1479.1, E(): 0, (41.9% identity in 623 aa overlap) and SW:YAY3_SCHPO hypothetical protein from Schizosaccharomyces pombe (649 aa) fasta scores; opt: 883, z-score: 1017.4, E(): 0, (33.1% identity in 634 aa overlap). 2 2 23 9 [Reporter]SCO3333 (14F11)_PCR/3011 [Gene]SCO3333/2742 NC_003888 SCE68.31c, possible hydrolase, len: 215 aa; similar to many e.g. TR:O68118 (EMBL:AF010496) Rhodobacter capsulatus hypothetical protein (225 aa), fasta scores; opt: 456 z-score: 540.0 E(): 9.4e-23, 38.5% identity in 221 aa overlap. Weakly similar to SW:GPH_RHOCA (EMBL:U23145), cbbZ, Rhodobacter capsulatus phosphoglycolate phosphatase (219 aa) (24.0% identity in 217 aa overlap). Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase 2 2 23 8 [Reporter]SCO0370 (15B11)_PCR/3010 [Gene]SCO0370/2741 NC_003888 SCF41.29c, possible DNA-binding protein (putative secreted protein), len: 1134 aa. Contains a possible N-terminal signal peptide sequence and a possible helix-turn-helix motif between residues 51..72 scores (+3.60 SD) 2 2 23 7 [Reporter]SCO3625 (16N7)_PCR/3009 [Gene]SCO3625/2740 NC_003888 SCH10.03c, hypothetical protein, len: 96 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 3 3 17 3 [Reporter]SCO7512 (19G22)_PCR/5394 [Gene]SCO7512/4919 NC_003888 SCBAC25F8.04c, probable AraC-family transcriptional regulator, len: 318 aa; highly similar to TR:Q53603 (EMBL:U21191) Streptomyces aureofaciens transcriptional activator GapR, 278 aa; fasta scores: opt: 1641 Z-score: 1807.9 bits: 342.5 E(): 4.3e-93; 88.849% identity in 278 aa overlap. Contains Pfam matches to entries PF01965 ThiJ, ThiJ/PfpI family and PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family and match to Prosite entry PS00041 Bacterial regulatory proteins, araC family signature. Also contains possible helix-turn-helix motif at residues 229..250 (+3.56 SD) 3 3 17 2 [Reporter]SCO7482 (20O18)_PCR/5393 [Gene]SCO7482/4918 NC_003888 SCBAC17A6.15, possible integral membrane protein, len: 133aa: appears to have a motif in common with others eg. TR:Q9L4T7 (EMBL:AF265258) dolichyl-phosphate beta-glucosyltransferase from Rhodococcus sp. S9 (449 aa) fasta scores; opt: 215, Z-score: 268.3, 40.310% identity (41.935% ungapped) in 129 aa overlap. Contains possible membrane-spanning hydrophobic regions. 3 3 16 22 [Reporter]SCO1212 (1O14)_PCR/5391 [Gene]SCO1212/4917 NC_003888 2SCG58.12, possible ligase, len: 412 aa; similar to TR:Q9ZGG7 (EMBL:AF080002) Heliobacillus mobilis UDP-N-acetylmuramyl tripeptide synthetase MurC, 455 aa; fasta scores: opt: 261 z-score: 287.4 E(): 1.5e-08; 29.3% identity in 441 aa overlap. Contains Pfam match to entry PF01225 Mur_ligase, Mur ligase family 3 3 16 21 [Reporter]SCO2298 (2K14)_PCR/5390 [Gene]SCO2298/4916 NC_003888 SCC30.06, kdgA, KHG/KDPG aldolase, len: 219 aa; highly similar to SW:ALKH_ECOLI (EMBL:X68871) Escherichia coli KHG/KDPG aldolase [includes: 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) (2-keto-4-hydroxyglutarate aldolase) (KHG-aldolase); 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14) (phospho-2-dehydro-3-deoxygluconate aldolase) (phospho-2-keto-3-deoxygluconate aldolase) (2-keto-3-deoxy-6-phosphogluconate aldolase) (KDPG-aldolase)] KdgA, 213 aa; fasta scores: opt: 757 z-score: 811.9 E(): 0; 55.0% identity in 202 aa overlap. Contains Pfam match to entry PF01081 Aldolase, KDPG and KHG aldolase and matches to Prosite entries PS00159 KDPG and KHG aldolases active site and PS00160 KDPG and KHG aldolases Schiff-base forming residue 3 3 16 20 [Reporter]SCO2223 (3G14)_PCR/5389 [Gene]SCO2223/4915 NC_003888 SC10B7,18, possible tetR-family transcriptional regulator, len: 230 aa; similar to TR:Q9XAE5 (EMBL:AL079356) Streptomyces coelicolor hypothetical 24.2 kD protein SC6G9.37, 217 aa; fasta scores: opt: 688 z-score: 811.4 E(): 0; 52.4% identity in 210 aa overlap and to TR:Q9WZT0 (EMBL:AE001749) Thermotoga maritima transcriptional regulator, tetR family, TM0823, 190 aa; fasta scores: opt: 169 z-score: 208.1 E(): 0.00033; 32.6% identity in 89 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a possible helix-turn-helix motif at residues 34..55 (+4.48 SD) 3 3 16 19 [Reporter]SCO5482 (4C14)_PCR/5388 [Gene]SCO5482/4914 NC_003888 SC2A11.16, unknown, len: 340 aa; some similarity to M. tuberculosis hypothetical protein YV25_MYCTU MTCY20G9.25, Rv0499 (291 aa), fasta scores; opt: 240 z-score: 316.7 E(): 2.2e-10, 28.9% identity in 270 aa overlap 3 3 16 18 [Reporter]SCO2096 (5O10)_PCR/5387 [Gene]SCO2096/4913 NC_003888 SC4A10.29, possible membrane protein, len: 797 aa; unknown function, shows weak similarity to parts of other bacterial hypothetical proteins e.g. TR:P94476 (EMBL:U51115), yebA, Bacillus subtilis hypothetical protein (655 aa), fasta scores; opt: 335 z-score: 330.0 E(): 4.7e-11, 30.4% identity in 263 aa overlap. Contains hydrophobic, possible membrane-spanning regions in the N-terminus 3 3 16 17 [Reporter]SCO6519 (6K10)_PCR/5386 [Gene]SCO6519/4912 NC_003888 SC5C7.04, possible lyase, similar to e.g. LGUL_HAEIN lactoylglutathione lyase (EC 4.4.1.5) (135 aa), fasta scores; opt: 124 z-score: 215.2 E(): 9.7e-05, 29.0% identity in 131 aa overlap 2 2 23 6 [Reporter]SCO1712 (17J7)_PCR/3008 [Gene]SCO1712/2739 NC_003888 SCI11.01c, possible tetR-family transcriptional regulator, partial CDS, len: >158 aa; weakly similar to many plasmid tetracycline repressor proteins e.g. SW:TER1_ECOLI (EMBL:X61367), TetR, Escherichia coli plasmid RP1 (216 aa), fasta scores; opt: 150 z-score: 196.6 E(): 0.0013, 32.8% identity in 122 aa overlap. Also similar to TR:O54102 (EMBL:AL021529) S.coelicolor putative transcriptional regulator (198 aa) (31.9% identity in 72 aa overlap). An alternative start codon is present at codon 4. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family,SCI30A.33c, partial CDS, unknown, len: >80 aa 3 3 16 16 [Reporter]SCO5690 (7G10)_PCR/5385 [Gene]SCO5690/4911 NC_003888 SC5H4.14, hypothetical protein, len: 747 aa; similar to TR:CAB36703 (EMBL:AL035521) Arabidopsis thaliana hypothetical 94.0 kD protein AT4G34260, 847 aa; fasta scores: opt: 322 z-score: 354.9 E(): 2.6e-12; 25.6% identity in 754 aa overlap 2 2 23 5 [Reporter]SCO0235 (18F7)_PCR/3007 [Gene]SCO0235/2738 NC_003888 SCJ9A.14, possible oxidoreductase, len: 312 aa; similar to many proposed oxidoreductases e.g. TR:O53537 (EMBL:AL021925) putative oxidoreductase from Mycobacterium tuberculosis (317 aa) fasta scores; opt: 988, z-score: 1030.4, E(): 0, (52.6% identity in 304 aa overlap). Also similar to SCJ9A.19c five CDSs downstream (311 aa) fasta scores; opt: 433, z-score: 409.8, E(): 1.4e-17, (38.0% identity in 300 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase 3 3 16 15 [Reporter]SCO5312 (8C10)_PCR/5384 [Gene]SCO5312/4910 NC_003888 SC6G9.21, conserved hypothetical protein, len: 144 aa; unknown function, similar to hypothetical proteins from Mycobacterium tuberculosis e.g. TR:O06553 (EMBL:Z95584) Mycobacterium tuberculosis hypothetical protein (147 aa), fasta scores; opt: 478 z-score: 561.4 E(): 6.1e-24, 55.7% identity in 140 aa overlap 2 2 23 4 [Reporter]SCP1.190c (19B7)_PCR/3006 [Gene]SCP1.190c/2737 NC_003888 SCP1.190c, possible integrase/recombinase, len: 277aa; similar to many eg. SW:P20184 (YT23_STRFR) hypothetical 23.1 kDa protein in transposon Tn4556 from Streptomyces fradiae (205 aa) fasta scores; opt: 559, z-score: 616.8, E(): 6.9e-27, 78.4% identity in 111 aa overlap and TR:O30680 (EMBL:AF015088) putative plasmid multimer resolution protein from plasmid pFAJ2600 of Rhodococcus erythropolis (306 aa) fasta scores; opt: 276, z-score: 308.0, E(): 1.1e-09, 33.9% identity in 227 aa overlap. 2 2 23 3 [Reporter]SCO6307 (20N3)_PCR/3005 [Gene]SCO6307/2736 NC_003888 SCIF3.09c, putative cyclase, len: 320 aa; similar to TRNEW:AAK64254 (EMBL:AF373840) hypothetical polyketide cyclase from nicotine degradation cluster of Arthrobacter nicotinovorans Plasmid pA01 (310 aa) fasta scores; opt: 394, Z-score: 442.9, E(): 4.9e-17, 31.000% identity (32.517% ungapped) in 300 aa overlap. 2 2 22 22 [Reporter]SCO1093 (1F23)_PCR/3003 [Gene]SCO1093/2735 NC_003888 2SCG4.09, possible hydroxylase, len: 283 aa; similar to TR:O50527 (EMBL:AL009204) Streptomyces coelicolor putative hydroxylase SC9B10.20c, 267 aa; fasta scores: opt: 534 z-score: 593.2 E(): 1.5e-25; 37.0% identity in 270 aa overlap. Contains a TTA leucine codon, possible target for bldA regulation 2 2 22 21 [Reporter]SCO7428 (2B23)_PCR/3002 [Gene]SCO7428/2734 NC_003888 SC6D11.24, hmpA1, possible flavohemoprotein, len: 398 aa. Highly similar to many protein of the flavohemoprotein subfamily, including: Escherichia coli SW:HMPA_ECOLI(EMBL:X58872) flavohemoprotein (hemoglobin-like protein) (396 aa), fasta scores: opt: 826 z-score: 935.2 E():0 39.4% identity in 404 aa overlap and Erwinia chrysanthemi SW:HMPA_ERWCH (EMBL:X75893) flavohemoprotein (395 aa), fasta scores: opt: 761 z-score: 862.0 E():0 35.9% identity in 404 aa overlap. Contains Pfam matches to entries Pfam match to entry PF00042 globin, Globin and Pfam match to entry PF00175 oxidored_fad, Oxidoreductase FAD/NAD-binding domain. 2 2 22 20 [Reporter]SCO6432 (3N19)_PCR/3001 [Gene]SCO6432/2733 NC_003888 SC1A6.21, probable peptide synthase, len: 1407 aa; similar to many e.g. TR:Q54959 (EMBL:X98690) Streptomyces pristinaespiralis Pristinamycin I synthase 2 (2591 aa), fasta scores; opt: 743 z-score: 754.8 E(): 0, 33.1% identity in1298 aa overlap. Contains PS00455 Putative AMP-binding domain signature, PS00012 Phosphopantetheine attachment site, PS00120 Lipases, serine active site and Pfam match to entry AMP-binding PF00501, AMP-binding enzymes, score 430.73 2 2 22 19 [Reporter]SCO7616 (4J19)_PCR/3000 [Gene]SCO7616/2732 NC_003888 SC2H2.14, possible AbaA-like protein, len: 89 aa; similar to TR:Q53896 (EMBL:X60316) Streptomyces coelicolor putative regulator AbaA or SCF42.13, 75 aa; fasta scores: opt: 209 z-score: 274.1 E(): 9.8e-08; 47.3% identity in 74 aa overlap 2 2 22 18 [Reporter]SCO7103 (5F19)_PCR/2999 [Gene]SCO7103/2731 NC_003888 SC4B10.04c, unknown, len: 99 aa. Highly hydrophilic. Contains a possible coiled-coil regions at approx, residues 41..59 2 2 22 16 [Reporter]SCO1443 (7N15)_PCR/2997 [Gene]SCO1443/2730 NC_003888 SC6D7A.06c, probable riboflavin synthase, len: 200 aa; similar to SW:RISA_ACTPL (EMBL:U27202) Actinobacillus pleuropneumoniae riboflavin synthase alpha chain (EC 2.5.1.9) RibE or RibB, 215 aa; fasta scores: opt: 607 z-score: 734.5 E(): 0; 50.3% identity in 193 aa overlap. Contains 2x Pfam matches to entry PF00677 Lum_binding, Lumazine binding domain and match to Prosite entry PS00693 Riboflavin synthase alpha chain family signature 3 3 16 14 [Reporter]SCO7839 (9O6)_PCR/5383 [Gene]SCO7839/4909 NC_003888 SC8E7.36, unknown, len: 116 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1Y4(EMBL:AL109973) hypothetical protein, SCJ30.03c (116 aa). 3 3 16 13 [Reporter]SCO2534 (10K6)_PCR/5382 [Gene]SCO2534/4908 NC_003888 SCC77.01, unknown, partial CDS, len: > 110 aa. Highly similar to a protein of undefined function from Mycobacterium tuberculosis SW:Y1DE_MYCTU(EMBL:Z95208) (435 aa), fasta scores opt: 290 z-score: 369.6 E(): 3.4e-13 54.0% identity in 100 aa overlap. Overlaps and extends into CDS SCC117.07 on the adjoining cosmid.,SCC117.07, possible membrane protein, partial CDS, len: > 358 aa. Highly similar to several proteins of undefined function e.g. Mycobacterium tuberculosis SW:YN66_MYCTU(EMBL:Z95208) hypothetical 47.2 KD protein (435 aa), fasta scores opt: 1159 z-score: 1367.6 E(): 0 53.0% identity in 353 aa overlap and Thermotoga maritima TR:Q9WZU9(EMBL:AE001751) hemolysin-related protein (455 aa), fasta scores opt: 568 z-score: 671.7 E(): 5.1e-30 29.9% identity in 355 aa overlap. Contains a Pfam match to entry PF01595 DUF21, Domain of unknown function and 2xPfam matches to entry PF00571 CBS, CBS domain. Contains a possible N-terminal signal sequence and membrane spanning hydrophobic domains. Overlaps and extends into CDS SCC77.01 on the adjoining cosmid. 3 3 16 11 [Reporter]SCO4803 (12C6)_PCR/5380 [Gene]SCO4803/4907 NC_003888 SCD63A.14, conserved hypothetical protein, len: 373 aa; similar to TR:CAB61595 (EMBL:AL133210) Streptomyces coelicolor hypothetical 41.5 kD protein SCG11A.14, 389 aa; fasta scores: opt: 774 z-score: 864.5 E(): 0; 46.5% identity in 376 aa overlap and to SW:YEHL_ECOLI (EMBL:U00007) Escherichia coli hypothetical 42.4 kD protein in MolR-BglX intergenic region YehL, 384 aa; fasta scores: opt: 771 z-score: 861.2 E(): 0; 41.2% identity in 359 aa overlap 3 3 16 10 [Reporter]SCO3037 (13O2)_PCR/5379 [Gene]SCO3037/4906 NC_003888 SCE34.18, possible oxidoreductase, len: 443 aa; similar to TR:P96867 (EMBL:Z92771) Mycobacterium tuberculosis hypothetical 47.6 kD protein, 448 aa; fasta scores: opt: 1180 z-score: 1285.8 E(): 0; 51.7% identity in 433 aa overlap. Contains Pfam match to entry PF01996 DUF129, Protein of unknown function and PF00881 Nitroreductase, Nitroreductase family 3 3 16 9 [Reporter]SCO0220 (14K2)_PCR/5378 [Gene]SCO0220/4905 NC_003888 SCJ12.32, unknown, len: 147 aa. 3 3 16 8 [Reporter]SCO0452 (15G2)_PCR/5377 [Gene]SCO0452/4904 NC_003888 SCF51A.30, possible SIR2-like regulatory protein, len: 299 aa. Similar to several Eukaryotic proteins e.g. Homo sapiens TR:AAD40852 (EMBL; AF083109) sirtuin type 4 (314 aa), fasta scores opt: 736 z-score: 829.3 E(): 0 46.3% identity in 255 aa overlap. Also similar to several Prokaryotic proteins e.g. Archaeoglobus fulgidus TR:O30124 (EMBL; AE001098) transcriptional regulatory protein, SIR2 family (253 aa), fasta scores opt: 319 z-score: 364.7 E(): 5.9e-13 31.9% identity in 257 aa overlap. 3 3 16 7 [Reporter]SCO0450 (16C2)_PCR/5376 [Gene]SCO0450/4903 NC_003888 SCF51A.28c, possible integral membrane protein, len: 326 aa. Weakly similar to a number of sensory transduction histidine kinases e.g. Synechocystis sp. (strain PCC 6803) TR:P72791 (EMBL; D90900) (834 aa), fasta scores opt: 172 z-score: 187.7 E(): 0.0042 23.7% identity in 317 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 3 3 16 6 [Reporter]SCO0037 (16O22)_PCR/5375 [Gene]SCO0037/4902 NC_003888 SCJ4.18c, possible sigma factor, len: 250 aa; similar to many proposed sigma factors e.g. TR:CAB44418 (EMBL:AL078610) putative sigma factor from Streptomyces coelicolor (223 aa) fasta scores; opt: 419, z-score: 506.3, E(): 7.7e-21, (44.3% identity in 183 aa overlap) and SW:SIGY_BACSU RNA polymerase sigma factor, SigY from Bacillus subtilis (178 aa) fasta scores; opt: 111, z-score: 142.5, E(): 1.4, (23.9% identity in 117 aa overlap). Note possible framshift from previous CDS as similar to all but the N-terminus of TR:CAB42013 (EMBL:AL049754) putative RNA polymerase sigma factor from Streptomyces coelicolor (294 aa) fasta scores; opt: 270, z-score: 327.8, E(): 6.7e-11, (26.9% identity in 238 aa overlap) while SCJ4.19c is similar to the N-terminus. Contains possible helix-turn-helix motif (+3.23 SD) 91-112 aa 2 2 22 15 [Reporter]SCO1999 (8J15)_PCR/2996 [Gene]SCO1999/2729 NC_003888 SC7H2.13, hypothetical protein, len: 220 aa; similar to many some of which are proposed to have methyltransferase activity egs. TR:CAB44537 (EMBL:AL078618) putative ubiquinone/menaquinone methyltransferase from Streptomyces coelicolor (231 aa) fasta scores; opt: 199, z-score: 247.0, E(): 2e-06, (40.4% identity in 104 aa overlap) and TR:O06424 (EMBL:Z95558) hypothetical protein from Mycobacterium tuberculosis (234 aa) fasta scores; opt: 191, z-score: 237.3, E(): 6.8e-06, (42.7% identity in 96 aa overlap). 3 3 16 5 [Reporter]SCO0908 (17K22)_PCR/5374 [Gene]SCO0908/4901 NC_003888 SCM1.41, unknown, len: 154 aa. Similar to the C-terminus of a protein of unknown function in Mycobacterium tuberculosis TR:P71738(EMBL:Z81368) (189 aa), fasta scores opt: 451 z-score: 544.4 E(): 5.6e-23 48.7% identity in 154 aa overlap 2 2 22 13 [Reporter]SCO1940 (10B15)_PCR/2994 [Gene]SCO1940/2728 NC_003888 SCC22.22c, possible secreted protein, len: 255 aa; weakly similar to hypothetical proteins e.g. TR:O33272 (EMBL:Z83018) hypothetical protein from Mycobacterium tuberculosis (255 aa) fasta scores; opt: 181, z-score: 213.5, E(): 0.00015, (27.0% identity in 226 aa overlap. Contains Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence 3 3 16 4 [Reporter]SCO0949 (18G22)_PCR/5373 [Gene]SCO0949/4900 NC_003888 SCM11.04c, hypothetical protein, len: 415 aa; similar to TR:CAB56687 (EMBL:AL121596) Streptomyces coelicolor hypothetical 71.4 kD protein SCF51A.35, 644 aa; fasta scores: opt: 205 z-score: 234.7 E(): 1.1e-05; 26.0% identity in 408 aa overlap 2 2 22 12 [Reporter]SCO4345 (11N11)_PCR/2993 [Gene]SCO4345/2727 NC_003888 SCD12A.28, unknown, len: 193 aa 2 2 22 11 [Reporter]SCO4156 (12J11)_PCR/2992 [Gene]SCO4156/2726 NC_003888 SCD84.23c, probable two-component systen response regulator, len: 248 aa; similar to SW:COPR_PSESM (EMBL:L05176) Pseudomonas syringae transcriptional activator protein CopR, 227 aa; fasta scores: opt: 610 z-score: 735.3 E(): 0; 42.7% identity in 227 aa overlap and to TR:O53894 (EMBL:AL021999) Mycobacterium tuberculosis putative two-component response regulator MTV044.09, 230 aa; fasta scores: opt: 933 z-score: 1119.0 E(): 0; 62.9% identity in 229 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 2 2 22 10 [Reporter]SCO3007 (13F11)_PCR/2991 [Gene]SCO3007/2725 NC_003888 SCE33.09, hypothetical protein, len: 391 aa; similar to TR:AAF11622 (EMBL:AE002043) Deinococcus radiodurans conserved hypothetical protein DR2072, 360 aa; fasta scores: opt: 300 z-score: 355.4 E(): 2.5e-12; 30.1% identity in 362 aa overlap and to TR:Q9HZZ9 (EMBL:AE004711) Pseudomonas aeruginosa hypothetical protein PA2844, 402 aa; fasta scores: opt: 629 Z-score: 729.7 E(): 5.2e-33; 40.415% identity in 386 aa overlap 2 2 22 9 [Reporter]SCO0622 (14B11)_PCR/2990 [Gene]SCO0622/2724 NC_003888 SCF56.06, probable tetR-family transcriptional regulator, len: 213 aa; similar to TR:CAB58268 (EMBL:AL121849) Streptomyces coelicolor putative tetR family transcriptional regulator SCF6.04, 180 aa; fasta scores: opt: 240 z-score: 284.5 E(): 1.8e-08; 34.5% identity in 177 aa overlap and to SW:TCMR_STRG (EMBL:M80674) Streptomyces glaucescens tetracenomycin C transcriptional repressor TcmR, 226 aa; fasta scores: opt: 212 z-score: 251.4 E(): 1.3e-06; 35.2% identity in 182 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a possible helix-turn-helix motif at residues 42..63 (+2.60 SD) 2 2 22 8 [Reporter]SCO0625 (15N7)_PCR/2989 [Gene]SCO0625/2723 NC_003888 SCF56.09c, possible transporter protein, len: 426 aa; low similarity to TR:Q9ZEJ2 (EMBL:AJ132740) Wolinella succinogenes large integral C4-dicarboxylate membrane transport protein, putative DctM, 415 aa; fasta scores: opt: 186 z-score: 201.0 E(): 0.00082; 23.7% identity in 427 aa overlap and to SW:IDNT_ECOLI (EMBL:U14003) Escherichia coli GntII-system L-idonate transporter (L-ido transporter) IdnT, 439 aa; fasta scores: opt: 176 z-score: 190.3 E(): 0.0032; 24.8% identity in 432 aa overlap 2 2 22 7 [Reporter]SCO0073 (16J7)_PCR/2988 [Gene]SCO0073/2722 NC_003888 SCJ11.02, unknown, len: 106 aa 2 2 22 6 [Reporter]SCO3493 (17F7)_PCR/2987 [Gene]SCO3493/2721 NC_003888 SCE65.29c, possible acetyl transferase, len: 195 aa; similar to TR:AAD15402 (EMBL:AC006223) Arabidopsis thaliana putative alanine acetyl transferase F22D22.23, 183 aa; fasta scores: opt: 208 z-score: 273.8 E(): 7.1e-08; 30.8% identity in 172 aa overlap and to SW:ATDA_ECOLI (EMBL:D25276) Escherichia coli spermidine N1-acetyltransferase (EC 2.3.1.57) SpeG, 185 aa; fasta scores: opt: 163 z-score: 216.7 E(): 0.00011; 23.6% identity in 174 aa overlap. Contains match to Pfam entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 2 2 22 4 [Reporter]SCP1.140 (19N3)_PCR/2985 [Gene]SCP1.140/2720 NC_003888 SCP1.140, possible membrane protein, len: 573aa; N-terminal half contains possible membrane-spanning hydrophobic regions and is strongly similar to the N-terminal region of of TR:Q9X894 (EMBL:AL049707) putative integral membrane protein from Streptomyces coelicolor (433 aa) fasta scores; opt: 1482, z-score: 1388.5, E(): 0, 62.4% identity in 420 aa overlap. 2 2 22 3 [Reporter]SCO1303 (20J3)_PCR/2984 [Gene]SCO1303/2719 NC_003888 SCBAC36F5.14c, unknown, len: 267 aa 2 2 21 22 [Reporter]SCO1089 (1B23)_PCR/2981 [Gene]SCO1089/2718 NC_003888 2SCG4.05c, unknown, len: 467 aa. High content in alanine, asparagine and valine amino acid residues 2 2 21 21 [Reporter]SCO2611 (2N19)_PCR/2980 [Gene]SCO2611/2717 NC_003888 SCC88.22c mreB, rod shape-determining protein, len: 343 aa; identical to previously sequenced TR:O33619 (EMBL:Y14206) Streptomyces coelicolor MreB protein, 342 aa and similar to SW:MREB_BACSU (EMBL:M95582) Bacillus subtilis rod shape-determining protein MreB, 337 aa; fasta scores: opt: 1346 z-score: 1516.0 E(): 0; 63.0% identity in 330 aa overlap 2 2 21 20 [Reporter]SCO6428 (3J19)_PCR/2979 [Gene]SCO6428/2716 NC_003888 SC1A6.17c, possible secreted protein, len: 2314 aa; has short segment of similarity to acid phosphatases e.g. PHOC_MORMO P28581 morganella morganii acid phosphatase (249aa), fasta scores; opt: 251 z-score: 206.3 E(): 0.00023, 32.1% identity in 162 aa overlap and to TR:CAC37553 (EMBL:AL590983) Streptomyces coelicolor conserved hypothetical protein (fragment) SCBAC1C11.07c, 676 aa; fasta scores: opt: 490 Z-score: 458.2E(): 6.9e-18; 26.124% identity in 712 aa overlap. Contains possible N-terminalsignal sequence and PS01157 Class A bacterial acid phosphatases signature 2 2 21 19 [Reporter]SCO5616 (4F19)_PCR/2978 [Gene]SCO5616/2715 NC_003888 SC2E1.33, unknown prophage gene, len: 162 aa; contains probable helix-turn-helix motif at aa 11 to 32 (Score 1192, +3.25 SD) 2 2 21 18 [Reporter]SCO6831 (5B19)_PCR/2977 [Gene]SCO6831/2714 NC_003888 SC4A9.08, unknown, len: 91 aa. 2 2 21 17 [Reporter]SCO2187 (6N15)_PCR/2976 [Gene]SCO2187/2713 NC_003888 SC5F7.14c, unknown, len: 308aa; similar to TR:O86334 (EMBL:Z97188) from Mycobacterium tuberculosis (539 aa) fasta scores; opt: 472, z-score: 514.3, E(): 2.6e-21, (37.5% identity in 208 aa overlap) 2 2 21 16 [Reporter]SCO1409 (7J15)_PCR/2975 [Gene]SCO1409/2712 NC_003888 SC6D7.30, possible membrane protein, len: 508 aa. Contains a possible membrane spanning hydrophobic domain and a probable coiled-coil between residues 259..286 (28 residues) 2 2 21 15 [Reporter]SCO5232 (8F15)_PCR/2974 [Gene]SCO5232/2711 NC_003888 SC7E4.29, possible sugar transporter sugar binding protein, len: 425 aa; similar to TR:Q9RK79 (EMBL:AL132662) Streptomyces coelicolor putative sugar transporter sugar binding protein SCF11.11, 422 aa; fasta scores: opt: 917 z-score: 991.5 E(): 0; 37.9% identity in 417 aa overlap and to SW:MALX_STRPN (EMBL:L08611) Streptococcus pneumoniae maltose/maltodextrin-binding protein precursor MalX, 423 aa; fasta scores: opt: 312 z-score: 340.7 E(): 1.7e-11; 22.9% identity in 428 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide sequence 2 2 21 14 [Reporter]SCO7532 (9B15)_PCR/2973 [Gene]SCO7532/2710 NC_003888 SC8G12.08c, possible secreted protein, len: 279 aa. Weakly similar to Streptomyces coelicolor TR:O69870(EMBL:AL023702) putative lipoprotein, SC1C3.28C (327 aa), fasta scores 70 opt: 137 z-score: 153.5 E(): 0.43 26.0% identity in 269 aa overlap. Contains a possible N-terminal signal sequence. 2 2 21 13 [Reporter]SCO2581 (10N11)_PCR/2972 [Gene]SCO2581/2709 NC_003888 SCC123.19c, possible membrane protein, len: 64 aa. Contains a possible membrane spanning hydrophobic domain. 2 2 21 12 [Reporter]SCO4337 (11J11)_PCR/2971 [Gene]SCO4337/2708 NC_003888 SCD12A.20, possible integral membrane efflux protein, len: 459 aa; similar to TR:Q9X863 (EMBL:AL049661) Streptomyces coelicolor putative integral membrane efflux protein SCE134.08, 500 aa; fasta scores: opt: 2083 z-score: 2154.5 E(): 0; 71.1% identity in 450 aa overlap and to TR:Q50396 (EMBL:U46844) Mycobacterium smegmatis ethambutanol resistance region EmbC, 412 aa; fasta scores: opt: 844 z-score: 878.0 E(): 0; 38.8% identity in 399 aa overlap. Contains possible hydrophobic membrane spanning regions 2 2 21 11 [Reporter]SCO4177 (12F11)_PCR/2970 [Gene]SCO4177/2707 NC_003888 SCD66.14, conserved hypothetical protein, len: 89 aa; similar to TR:CAB69735 (EMBL:AL137166) Streptomyces coelicolor hypothetical 10.2 kD protein SCC121.17, 96 aa; fasta scores: opt: 288 z-score: 395.7 E(): 1.4e-14; 50.0% identity in 78 aa overlap. Contains 2x degenerate repeat units: WRRSSYSN 2 2 21 10 [Reporter]SCO3002 (13B11)_PCR/2969 [Gene]SCO3002/2706 NC_003888 SCE33.04c, unknown, len: 95 aa 2 2 21 9 [Reporter]SCO2893 (14N7)_PCR/2968 [Gene]SCO2893/2705 NC_003888 SCE6.30c, unknown, len: 277 aa. Similar to several proteins of undefined function e.g. Mycobacterium leprae TR:Q9Z5H1(EMBL:AL035500) hypothetical 22.1 kd protein (200 aa), fasta scores opt: 413 z-score: 443.6 E(): 2.8e-17 41.5% identity in 205 aa overlap. Contains a possible coiled-coil between residues 206..226. 2 2 21 8 [Reporter]SCO3710 (15J7)_PCR/2967 [Gene]SCO3710/2704 NC_003888 SCH35.14c, possible large integral membrane protein, len: 544aa; Contains possible membrane spanning hydrophobic regions. Region (282-346aa) of similarity to SW:YZ25_MYCTU hypothetical protein from from Mycobacterium tuberculosis (535aa) blast scores: Identities = 32/69 (46%), Positives = 37/69 (53%). 2 2 21 7 [Reporter]SCO3563 (16F7)_PCR/2966 [Gene]SCO3563/2703 NC_003888 SCH5.26, acsA, acetyl-coenzyme A synthetase, len: 651 aa; strong similarity to many egs. TR:O69635 (EMBL:AL022121) Acs, acetyl-coenzyme A synthetase from Mycobacterium tuberculosis (651 aa) fasta scores; opt: 2850, z-score: 3300.6, E(): 0, (66.0% identity in 639 aa overlap) and SW:ACSA_ECOLI AcsA, acetyl-coenzyme A synthetase from Escherichia coli (652 aa) fasta scores; opt: 2230, z-score: 2582.2, E(): 0, (52.6% identity in 645 aa overlap). Contains PS00455 Putative AMP-binding domain signature and Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme, score 417.30, E-value 1.4e-121. 2 2 21 6 [Reporter]SCO3576 (17B7)_PCR/2965 [Gene]SCO3576/2702 NC_003888 SCH17.10c, hypothetical protein, len: 53 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 2 2 21 5 [Reporter]SCO3882 (18N3)_PCR/2964 [Gene]SCO3882/2701 NC_003888 StH24.04, conserved hypothetical protein, len: 124 aa; previously sequenced as TR:O54625 (EMBL:Y16311) Streptomyces coelicolor hypothetical protein (124 aa). Similar to many bacterial proteins from RnpA region e.g. YIDD_PROMI (EMBL:M58352) Proteus mirabilis hypothetical protein (86 aa), fasta scores; opt: 278 z-score: 336.6 E(): 2e-11, 57.4% identity in 68 aa overlap. Also similar to haemolysins e.g. TR:Q44066 (EMBL:L36462), HylA, Aeromonas hydrophila alpha-hemolysin (85 aa) (54.5% identity in 66 aa overlap) 2 2 21 4 [Reporter]SCO4027 (19J3)_PCR/2963 [Gene]SCO4027/2700 NC_003888 2SC10A7.31c, probable anti sigma factor antagonist, len: 126 aa; similar to SW:RSBV_STRCO (EMBL:AF134889) Streptomyces coelicolor anti-sigma B factor antagonist RsbV or BldG, 113 aa; fasta scores: opt: 131 z-score: 175.0 E(): 0.03; 30.4% identity in 102 aa overlap. Contains Pfam match to entry PF01740 STAS, STAS domain 4 3 18 11 [Reporter]SCO3954 (12L6)_PCR/5928 [Gene]SCO3954/5399 NC_003888 SCD78.21c, putative oxidoreductase, len: 584 aa; N-terminal region similar to Y00P_MYCTU (EMBL:Z70283) putative oxidoreductase from Mycobacterium tuberculosis (650 aa), fasta scores; opt: 1457, z-score: 840.1, E(): 0, 41.0% identity in 652 aa overlap. Contains PS00061 short chain dehydrogenases /reductases family signature 4 3 18 10 [Reporter]SCO3077 (13H6)_PCR/5927 [Gene]SCO3077/5398 NC_003888 SCE25.18, probable cystathionine beta-synthase, len: 461 aa; highly similar to TR:O53425 (EMBL:AL021897) Mycobacterium tuberculosis cystathionine beta-synthase CysM, 464 aa; fasta scores: opt: 2025 z-score: 2184.8 E(): 0; 67.6% identity in 454 aa overlap, to TR:Q9YHU3 (EMBL:AF090120) Fugu rubripe cystathionine beta-synthase, 585 aa; fasta scores: opt: 1087 z-score: 1173.5 E(): 0; 39.6% identity in 465 aa overlap and to TR:CAB50991 (EMBL:AL096852) Streptomyces coelicolor putative cysteine synthase SCE19A.10c, 316 aa; fasta scores: opt: 558 z-score: 540.6 E(): 1.4e-24; 40.3% identity in 303 aa overlap. Contains Pfam matches to entries PF00291 S_T_dehydratase, Pyridoxal-phosphate dependent enzyme, 2x PF00571 CBS, CBS domain and match to Prosite entry PS00901 Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site 4 3 18 9 [Reporter]SCO1726 (14D6)_PCR/5926 [Gene]SCO1726/5397 NC_003888 SCI11.15, possible ATPase, len: 364 aa; unknown function, very weak similarity to eukaryotic putative ATPases e.g. TR:P78787 (EMBL:D89136) Schizosaccharomyces pombe putative ATPase (partial CDS) (419 aa), fasta scores; opt: 184 z-score: 216.6 E(): 9.9e-05, 26.5% identity in 309 aa overlap and to part of SW:AFG2_YEAST (EMBL:L14615), Agf2, Saccharomyces cerevisiae putative ATPase essential for viability (780 aa) (29.9% identity in 137 aa overlap). Contains weak Pfam match to entry PF00004 AAA, ATPases associated with various cellular activities (AAA) and PS00017 ATP/GTP-binding site motif A (P-loop) 4 3 18 8 [Reporter]SCO3701 (15P2)_PCR/5925 [Gene]SCO3701/5396 NC_003888 SCH35.23c, unknown, len: 76aa; similar to the N-terminus of many thioredoxin reductases from prokaryotes and eukaryotes egs. SW:TRXB_STRCO TrxB, thioredoxin reductase from Streptomyces coelicolor (319 aa) fasta scores; opt: 215, z-score: 280.7, E(): 2.6e-08, (63.2% identity in 57 aa overlap) and SW:TRXB_NEUCR Cys-9 thioredoxin reductase from Neurospora crassa (334 aa) fasta scores; opt: 158, z-score: 209.4, E(): 0.00025, (51.8% identity in 56 aa overlap). 4 3 18 7 [Reporter]SCO3675 (16L2)_PCR/5924 [Gene]SCO3675/5395 NC_003888 SCH35.49c, putative conserved secreted protein, len: 410aa; Similar to others from Streptomyces coelicolor. Contains a possible N-terminal signal sequence. 4 3 18 6 [Reporter]SCO0660 (17H2)_PCR/5923 [Gene]SCO0660/5394 NC_003888 SCF91.20, probable solute-binding lipoprotein, len: 443 aa. Similar to several solute-binding proteins e.g. Agrobacterium radiobacter SW:LACE_AGRRD(EMBL:X66596) lactose-binding protein precursor (422 aa), fasta scores opt: 270 z-score: 303.1 E(): 1.6e-09 23.5% identity in 387 aa overlap and Streptomyces coelicolor TR:P72397(EMBL:Y07706) putative maltose-binding protein MalE (423 aa), fasta scores opt: 345 z-score: 386.1 E(): 4e-14 25.5% identity in 459 aa overlap. Contains a Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein. Also contains an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. 4 3 18 4 [Reporter]SCP1.109 (18P22)_PCR/5921 [Gene]SCP1.109/5393 NC_003888 SCP1.109, unknown, len: 63aa; 4 3 18 3 [Reporter]SCO4003 (19L22)_PCR/5920 [Gene]SCO4003/5392 NC_003888 2SC10A7.07c, unknown, len: 117 aa 4 3 18 2 [Reporter]SCO5985 (20D22)_PCR/5919 [Gene]SCO5985/5391 NC_003888 SCBAC16H6.20, conserved hypothetical protein, len: 132aa: similar to many eg. TR:BAB51882 (EMBL:AP003006) MLL5429 protein from Rhizobium loti (137 aa) fasta scores; opt: 271, Z-score: 331.4, 34.884% identity (35.156% ungapped) in 129 aa overlap. Contains Pfam match to entry PF01042 UPF0076, YjgF family. 4 3 17 22 [Reporter]SCO1113 (1D18)_PCR/5917 [Gene]SCO1113/5390 NC_003888 2SCG38.06, conserved hypothetical protein, len: 384 aa; similar to TR:AAG04745 (EMBL:AE004565) Pseudomonas aeruginosa hypothetical 41.0 kDa protein PA1356, 368 aa; fasta scores: opt: 714 z-score: 833.2 E(): 0; 36.9% identity in 374 aa overlap. Contains 6x Pfam matches to entry PF02012 BNR, BNR repeat 4 3 17 21 [Reporter]SCO2528 (2P14)_PCR/5916 [Gene]SCO2528/5389 NC_003888 SCC121.31c, leuA, 2-ispoprylmalate synthase (EC 4.1.3.12), len: 499 aa; identical to C-terminal region of previously sequenced Streptomyces coelicolor 2-isopropylmalate synthase (EC 4.1.3.12) LeuA, 573 aa and highly similar to SW:LEU1_CORGL (EMBL:X70959) Corynebacterium glutamicum (Brevibacterium flavum) 2-isopropylmalate synthase (EC 4.1.3.12) (alpha-isopropylmalate synthase) (alpha-ipm synthetase) LeuA, 616 aa; fasta scores: opt: 1812 z-score: 2105.1 E(): 0; 55.1% identity in 506 aa overlap. Contains Pfam match to entry PF00682 HMGL-like and match to Prosite entry PS00816 Alpha-isopropylmalate and homocitrate synthases signature 2,SCC117.01c, leuA, 2-isopropylmalate synthase (EC 4.1.3.12) (fragment), len: > 113 aa. Previously sequenced and characterised Streptomyces coelicolor TR:O31046(EMBL:AF026444) (573 aa). Contains a Prosite hit to PS00815 Alpha-isopropylmalate and homocitrate synthases signature 1. Overlaps and extends into CDS SCC121.31c on the adjoining cosmid. 4 3 17 20 [Reporter]SCO6807 (3L14)_PCR/5915 [Gene]SCO6807/5388 NC_003888 SC1A2.16c, unknown, len: 597 aa. 4 3 17 19 [Reporter]SCO4816 (4H14)_PCR/5914 [Gene]SCO4816/5387 NC_003888 SC2A6.01c, hypothetical protein (fragment), len: >232 aa; similar to TR:O34320 (EMBL:AJ222587) Bacillus subtilis YkuG protein, 760 aa; fasta scores: opt: 439 z-score: 541.2 E(): 1.2e-22; 37.1% identity in 229 aa overlap,SCD63A.27c, hypothetical protein (fragment), len: >605 aa; similar to TR:O34320 (EMBL:AJ222587) Bacillus subtilis YkuG protein, 760 aa; fasta scores: opt: 961 z-score: 1162.8 E(): 0; 34.4% identity in 596 aa overlap 4 3 17 18 [Reporter]SCO2378 (5D14)_PCR/5913 [Gene]SCO2378/5386 NC_003888 SC4A7.06c, possible merR-family transciptional regulator, len: 196 aa; similar to SW:TIPA_STRLI (EMBL:S64314) Streptomyces lividans transcriptional activator TipA, 253 aa; fasta scores: opt: 187 z-score: 225.2 E(): 3.7e-05; 32.7% identity in 110 aa overlap. Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and a possible helix-turn-helix motif at residues 72..93 (+3.19 SD) 4 3 17 17 [Reporter]SCO6525 (6P10)_PCR/5912 [Gene]SCO6525/5385 NC_003888 SC5C7.10c, unknown, len: 255 aa; similar to hypothetical proteins from many organisms e.g. M. tuberculosis YZ34_MYCTU MTCY31.34 (372 aa), fasta scores; opt: 221 z-score: 293.2 E(): 4.4e-09, 30.7% identity in 251 aa overlap 4 3 17 16 [Reporter]SCO6785 (7L10)_PCR/5911 [Gene]SCO6785/5384 NC_003888 SC6A5.34, probable fatty acid co-A racemase, len: 387aa; similar to egs. TR:O06543 (EMBL:Z95584) hypothetical protein from Mycobacterium tuberculosis (360 aa) fasta scores; opt: 1304, z-score: 1373.1, E(): 0, (52.8% identity in 362 aa overlap) and TR:O09176 (EMBL:U89905) alpha-methylacyl-CoA racemase from rat liver (Rattus norvegicus) (361 aa) fasta scores; opt: 985, z-score: 1039.0, E(): 0, (44.4% identity in 342 aa overlap). 4 3 17 15 [Reporter]SCO5306 (8H10)_PCR/5910 [Gene]SCO5306/5383 NC_003888 SC6G9.27, possible protein phosphatase, len: 159 aa; very weak similarity to eukaryotic tyrosine and dual-specificity protein phosphatases e.g. TR:O70275 (EMBL:AF035645) Mus musculus protein tyrosine phosphatase 4A3 (173 aa), fasta scores; opt: 130 z-score: 173.4 E(): 0.025, 33.3% identity in 78 aa overlap. Alternative start codons are present at codons 12 and 17. Contains Pfam match to entry PF00782 DSPc, Dual specificity phosphatase, catalytic domain 4 3 17 14 [Reporter]SCO5504 (9D10)_PCR/5909 [Gene]SCO5504/5382 NC_003888 SC8D9.16, possible integral membrane protein, len: 208aa; alanine-rich, contains possible membrane spanning regions. 4 3 17 13 [Reporter]SCO2503 (10P6)_PCR/5908 [Gene]SCO2503/5381 NC_003888 SCC121.06, probable chitinase precursor, len: 358 aa; similar to C-terminal region of SW:CHID_BACCI (EMBL:D10594) Bacillus circulans chitinase D precursor (EC 3.2.1.14) ChiD, 497 aa; fasta scores: opt: 471 z-score: 502.4 E(): 1.4e-20; 33.0% identity in 370 aa overlap and to SW:CHIX_STROI (EMBL:X71080) Streptomyces olivaceoviridis (Streptomyces corchorusii exochitinase 1 precursor (EC 3.2.1.14) Chi01, 597 aa; fasta scores: opt: 249 z-score: 267.3 E(): 1.7e-07; 31.2% identity in 391 aa overlap. Contains Pfam match to entry PF00704 Glyco_hydro_18 and match to Prosite enry PS01095 Chitinases family 18 active site. Contains also possible N-terminal region signal peptide sequence 4 3 17 12 [Reporter]SCO4379 (11L6)_PCR/5907 [Gene]SCO4379/5380 NC_003888 SCD10.11, conserved hypothetical protein, len: 558 aa; similar to TR:O86320 (EMBL:Z79700) Mycobacterium tuberculosis hypothetical 59.1 kD protein aa; fasta scores: opt: 2549 z-score: 2481.8 E(): 0; 69.0% identity in 562 aa overlap 4 3 17 11 [Reporter]SCO4810 (12H6)_PCR/5906 [Gene]SCO4810/5379 NC_003888 SCD63A.21c, hypothetical protein, len: 342 aa; similar to TR:Q9Z9I2 (EMBL:AB024056) Streptomyces fradiae unknown protein, 306 aa; fasta scores: opt: 645 z-score: 632.8 E(): 8.7e-28; 44.5% identity in 281 aa overlap 4 3 17 10 [Reporter]SCO3072 (13D6)_PCR/5905 [Gene]SCO3072/5378 NC_003888 SCE25.13c, probable amino acid hydrolase, len: 400 aa; similar to SW:AMB2_BACST (EMBL:S67784) Bacillus stearothermophilus N-carbamyl-L-amino acid amidohydrolase (EC 3.5.1.-) AmaB, 409 aa; fasta scores: opt: 733 z-score: 804.8 E(): 0; 34.7% identity in 404 aa overlap 4 3 17 9 [Reporter]SCO0393 (14P2)_PCR/5904 [Gene]SCO0393/5377 NC_003888 SCF62.19, possible transferase, len: 269 aa. Similar to many transferases e.g. Streptomyces glaucescens TR:Q54266 (EMBL:X82177) alpha-D-glucose-1-phosphate cytidylyl-transferase StrQ (fragment) (299 aa), fasta scores opt: 554 z-score: 687.3 E(): 6.3e-31 35.0% identity in 280 aa overlap. 4 3 17 8 [Reporter]SCO3759 (15L2)_PCR/5903 [Gene]SCO3759/5376 NC_003888 SCH63.06c, possible integral membrane protein, len: 403 aa; similar to TR:Q9X8Q9 (EMBL:AL049754) Streptomyces coelicolor putative membrane protein SCH10.20c, 420 aa; fasta scores: opt: 898 z-score: 1025.8 E(): 0; 45.3% identity in 395 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 17 7 [Reporter]SCO0762 (16H2)_PCR/5902 [Gene]SCO0762/5375 NC_003888 SCF81.21c, sti1, protease inhibitor precursor, len: 144 aa; identical to SW:SSI_STRLI (EMBL:M80576) Streptomyces lividans protease inhibitor precursor (SlpI) (trypsin inhibitor) Sti1, 144 aa and highly similar to SW:SSI2_STRLO (EMBL:M80577) Streptomyces longisporus trypsin inhibitor Sti2 precursor, 144 aa; fasta scores: opt: 735 z-score: 837.0 E(): 0; 76.4% identity in 144 aa overlap. Contains match to Pfam entry PF00720 SSI, Subtilisin inhibitor-like and match to Prosite entry PS00999 Streptomyces subtilisin-type inhibitors signature 4 3 17 6 [Reporter]SCO0888 (17D2)_PCR/5901 [Gene]SCO0888/5374 NC_003888 SCM1.21, possible secreted protein, len: 187 aa. Similar to several proteins of undefined function e.g. Mycobacterium tuberculosis TR:P95105 (EMBL:Z83866) hypothetical 19.1 KD protein (184 aa), fasta scores opt: 651 z-score: 722.6 E(): 6.7e-33 56.6% identity in 175 aa overlap. Contains a possible N-terminal signal sequence 4 3 17 5 [Reporter]SCO0856 (17P22)_PCR/5900 [Gene]SCO0856/5373 NC_003888 SCM2.09c possible integral membrane protein, len: 408 aa. Contains multiple possible membrane spanning hydrophobic domains. Also contains a TTA /leucine codon, which is a possible target for bldA regulation. 4 3 17 3 [Reporter]SCO4992 (19H22)_PCR/5898 [Gene]SCO4992/5372 NC_003888 2SCK36.15, unknown, len: 214 aa. High content of alanine and glutamic amino acid residues in C-terminal region 3 2 23 10 [Reporter]SCO3445 (13K11)_PCR/3516 [Gene]SCO3445/3199 NC_003888 SCE46.02c, putative small membrane protein, len: 55 aa. Contains possible hydrophobic membrane spanning region 4 3 17 2 [Reporter]SCO5980 (20P18)_PCR/5897 [Gene]SCO5980/5371 NC_003888 SCBAC16H6.15, possible bifunctional hydroxylase/oxidoreductase, len: 801aa: N-terminal region similar to hydroxylases eg. TR:Q9S3U8 (EMBL:AF172851) hydroxylase VioD from Chromobacterium violaceum (373 aa) fasta scores; opt: 549, Z-score: 591.4, 31.768% identity (33.430% ungapped) in 362 aa overlap and TR:Q9X7R5 (EMBL:AL049863) putative hydroxylase SC5H1.07c from Streptomyces coelicolor (421 aa) fasta scores; opt: 957, Z-score: 1027.4, 44.473% identity (47.527% ungapped) in 389 aa overlap. C-terminal region similar to oxidoreductases eg. TR:Q9R9V9 (EMBL:AF154061) xenobiotic reductase XenA from Pseudomonas putida (363 aa) fasta scores; opt: 895, Z-score: 962.0, 39.831% identity (40.517% ungapped) in 354 aa overlap and TR:Q9EX17 (EMBL:AL445503) putative oxidoreductase from Streptomyces coelicolor (359 aa) fasta scores; opt: 1008, Z-score: 1083.0, 46.667% identity (48.276% ungapped) in 360 aa overlap. Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase; Pfam match to entry PF00724 oxidored_FMN, NADH:flavin oxidoreductase / NADH oxidase family; Prosite match to PS00133 Zinc carboxypeptidases, zinc-binding region 2 signature and Prosite match to PS00383 Tyrosine specific protein phosphatases active site. 3 2 23 9 [Reporter]SCO0861 (14G11)_PCR/3515 [Gene]SCO0861/3198 NC_003888 SCM2.14c, putative secreted protein, len: 69 aa. Rich in the amino acid Gly. Contains possible N-terminal region signal peptide sequence 4 3 16 22 [Reporter]SCO1213 (1P14)_PCR/5895 [Gene]SCO1213/5370 NC_003888 2SCG58.13, hypotetical protein, len: 242 aa; similar to TR:Q9ZGG8 (EMBL:AF080002) Heliobacillus mobilis cobyric acid synthase CobQ, 252 aa; fasta scores: opt: 505 z-score: 574.8 E(): 1.5e-24; 39.5% identity in 238 aa overlap 3 2 23 8 [Reporter]SCO1631 (15C11)_PCR/3514 [Gene]SCO1631/3197 NC_003888 SCI41.14c, possible helicase, len: 950 aa. Similar to TR:O69801 (EMBL:AJ223310) Streptomyces avermitilis SAB3 helicase, 612 aa; fasta scores: opt: 2382 z-score: 2658.3 E(): 0; 68.9% identity in 602 aa overlap. Contains Pfam match to entry PF00270 DEAD, DEAD/DEAH box helicase and PS00017 ATP/GTP-binding site motif A (P-loop). Contains probable coiled-coil from 554 to 586 (33 residues, max score: 1.477, probability 0.92). 3 2 23 7 [Reporter]SCO3872 (16O7)_PCR/3513 [Gene]SCO3872/3196 NC_003888 SCH18.09c, possible integral membrane protein, len: 185 aa; similar to TR:P71575 (EMBL:Z80233) Mycobacterium tuberculosis hypothetical 31.3 kD protein CY10H4.05, 304 aa; fasta scores: opt: 315 z-score: 339.9 E(): 1.8e-11; 32.8% identity in 195 aa overlap. Contains possible hydrophobic membrane spanning regions 3 2 23 6 [Reporter]SCO0145 (17K7)_PCR/3512 [Gene]SCO0145/3195 NC_003888 SCJ33.09, possible secreted protein, len: 342 aa. Similar to Streptomyces coelicolor TR:CAB45466 (EMBL; AL079348) hypothetical 33.9 KD protein SC66T3.10C (318 aa), fasta scores opt: 272 z-score: 301.5 E(): 2e-09 34.0% identity in 300 aa overlap. Contains a TTA /leucine codon, possible target for bldA regulation. Contains a possible N-terminal signal sequence. 3 2 23 4 [Reporter]SCP1.177 (19C7)_PCR/3510 [Gene]SCP1.177/3194 NC_003888 SCP1.177, possible membrane protein, len: 202aa; contains three possible membrane-spanning hydrophobic regions. This gene lies in a region of unusually low GC content. 3 2 23 3 [Reporter]SCP1.106 (20O3)_PCR/3509 [Gene]SCP1.106/3193 NC_003888 SCP1.106, unknown, len: 197aa; 3 2 22 22 [Reporter]SCO1094 (1G23)_PCR/3507 [Gene]SCO1094/3192 NC_003888 2SCG4.10, probable tetR-family regulatory protein, len: 192 aa; similar to TR:CAB69777 (EMBL:AL137187) Streptomyces coelicolor putative tetR-family transcriptional regulator SC7A8.28, 194 aa; fasta scores: opt: 419 z-score: 522.7 E(): 1.3e-21; 39.7% identity in 194 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 3 2 22 21 [Reporter]SCO7094 (2C23)_PCR/3506 [Gene]SCO7094/3191 NC_003888 SC3A4.20c, hmpA2, flavohemoprotein (hemoglobin-like protein), len: 403 aa. Highly similar to many including: Escherichia coli SW:HMPA_ECOLI (EMBL:X58872) flavohemoprotein, HmpA (396 aa), fasta scores: opt: 833 z-score: 947.5 E():0 37.9% identity in 406 aa overlap and Streptomyces coelicolor TR:CAB76347 (EMBL:AL158061) flavohemoprotein, HmpA1 or SC6D11.24 (398 aa), fasta scores: opt: 1706 z-score: 1936.1 E(): 0 65.3% identity in 404 aa overlap. contains Pfam matches to entries PF00175 oxidored_fad, Oxidoreductase FAD/NAD-binding domain and PF00042 globin, Globin. 3 2 22 20 [Reporter]SCO6433 (3O19)_PCR/3505 [Gene]SCO6433/3190 NC_003888 SC1A6.22, unknown, len: 135 aa 4 3 16 21 [Reporter]SCO5871 (2L14)_PCR/5894 [Gene]SCO5871/5369 NC_003888 SC2E9.12, kdpD, two-component sensor/protein kinase, len: 848 aa; similar to Escherichia coli turgor pressure sensor KDPD_ECOLI P21865 sensor protein kdpD (ec 2.7.3.-) (894 aa), fasta scores; opt: 997 z-score: 1347.4 E(): 0, 34.8% identity in 877 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and Pfam match to entry signal PF00512, Signal C terminal domain, score 184.57. Contains possible hydrophobic membrane spanning regions 4 3 16 20 [Reporter]SCO2224 (3H14)_PCR/5893 [Gene]SCO2224/5368 NC_003888 SC10B7.19, unknown, len: 154 aa. High content in alanine and proline amino acid residues. Also high G+C content (80.3%) 4 3 16 19 [Reporter]SCO5483 (4D14)_PCR/5892 [Gene]SCO5483/5367 NC_003888 SC2A11.17c, probable transcriptional regulator, len: 208 aa; similar to members of the tetR family e.g. TCMR_STRGA tetracenomycin C transcriptional repressor (226 aa), fasta scores; opt: 138 z-score: 224.5 E(): 3e-05, 26.5% identity in 155 aa overlap. Contains helix-turn-helix motif at aa 36-57 (Score 1332, +3.72 SD) and Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family, score 23.40, E-value 9.9e-05 4 3 16 18 [Reporter]SCO2097 (5P10)_PCR/5891 [Gene]SCO2097/5366 NC_003888 SC4A10.30c, possible membrane protein, len: 135 aa; unknown function, similar to mycobacterial hypothetical proteins TR:O53503 (EMBL:AL021957) Mycobacterium tuberculosis (134 aa), fasta scores; opt: 349 z-score: 414.6 E(): 9e-16, 44.1% identity in 136 aa overlap and its homologue in Mycobacterium leprae TR:O69562 (EMBL:AL022602) Mycobacterium leprae hypothetical protein (134 aa) (42.6% identity in 136 aa overlap). Contains hydrophobic, possible membrane-spanning regions 4 3 16 17 [Reporter]SCO6521 (6L10)_PCR/5890 [Gene]SCO6521/5365 NC_003888 SC5C7.06, probable secreted protein, len: 318 aa; contains N-terminal signal sequence 4 3 16 16 [Reporter]SCO5691 (7H10)_PCR/5889 [Gene]SCO5691/5364 NC_003888 SC5H4.15, probable secreted sugar hydrolase, len: 244 aa; similar to SW:GUNS_ERWCA (EMBL:M32399) Erwinia carotovora endoglucanase S precursor (EC 3.2.1.4) (endo-1,4-beta-glucanase S) (cellulase S) CelS, 264 aa; fasta scores: opt: 283 z-score: 330.2 E(): 6.2e-11; 33.2% identity in 253 aa overlap. Contains Pfam match to entry PF01670 Glyco_hydro_12, Glycosyl hydrolase family 12 and possible N-terminal region signal peptide sequence 4 3 16 15 [Reporter]SCO5310 (8D10)_PCR/5888 [Gene]SCO5310/5363 NC_003888 SC6G9.23, hypothetical protein, len: 187 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference, amino acid composition and the presence of a possible RBS 4 3 16 14 [Reporter]SCO7840 (9P6)_PCR/5887 [Gene]SCO7840/5362 NC_003888 SC8E7.37, unknown, len: 148 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1Y6 (EMBL:AL109973) hypothetical protein, SCJ30.02c (148 aa). 3 2 22 19 [Reporter]SCO7617 (4K19)_PCR/3504 [Gene]SCO7617/3189 NC_003888 SC2H2.15, hypothetical protein, len: 271 aa; similar to many Streptomyces coelicolor hypothetical proteins, e.g. TR:O86718 (EMBL:AL031515) hypothetical 29.3 kDa protein SC5C7.21c, 271 aa; fasta scores: opt: 863 z-score: 1030.6 E(): 0; 51.5% identity in 274 aa overlap 4 3 16 13 [Reporter]SCO2499 (10L6)_PCR/5886 [Gene]SCO2499/5361 NC_003888 SCC121.02c, possible transport ATPase, len: 1472 aa; similar to TR:P96271 (EMBL:Z84724) Mycobacterium tuberculosis hypothetical 159.8 kD protein MTCY22G10.22c, 1539 aa; fasta scores: opt: 2216 z-score: 2157.1 E(): 0; 38.4% identity in 1543 aa overlap and N-terminal region similar to SW:ATMA_SALTY (EMBL:U07843) Salmonella typhimurium Mg(2+) transport atpase, P-type 1 (EC 3.6.1.-) MgtA, 902 aa; fasta scores: opt: 800 z-score: 779.8 E(): 0 ;28.0% identity in 880 aa overlap. Contains Pfam match to entry PF00122 E1-E2_ATPase, match to Prosite entry PS00154 E1-E2 ATPases phosphorylation site and possible N-terminal region signal peptide sequence 3 2 22 18 [Reporter]SCO7104 (5G19)_PCR/3503 [Gene]SCO7104/3188 NC_003888 SC4B10.05, possible RNA polymerase sigma factor, len: 292 aa; similar to TR:CAB89771 (EMBL:AL354616) Streptomyces coelicolor putative RNA polymerase sigma factor SC3A4.25c, 300 aa; fasta scores: opt: 901 z-score: 941.4 E(): 0; 51.7% identity in 290 aa overlap 4 3 16 12 [Reporter]SCO2290 (11H6)_PCR/5885 [Gene]SCO2290/5360 NC_003888 SCC75A.36, unknown, len: 113 aa. Weakly similar to Bacillus subtilis SW:YPJD_BACSU(EMBL:L38424) hypothetical 13.0 KD protein (111 aa), fasta scores opt:103 z-score: 145.9 E():0.94 30.8% identity in 91 aa overlap. 3 2 22 17 [Reporter]SCO7561 (6C19)_PCR/3502 [Gene]SCO7561/3187 NC_003888 SC5F1.15c, possible esterase, len: 353 aa; similar to SW:PBP4_NOCLA (EMBL:Z13972) Nocardia lactamdurans penicillin-binding protein 4 Pbp-4, 381 aa; fasta scores: opt: 246 z-score: 283.9 E(): 2.8e-08; 30.6% identity in 301 aa overlap 3 2 22 16 [Reporter]SCO2108 (7O15)_PCR/3501 [Gene]SCO2108/3186 NC_003888 SC6E10.02, conserved hypothetical protein, len: 66 aa; unknown function, similar to e.g. SW:THIS_ECOLI (EMBL:M88701), ThiS, ThiG1, Escherichia coli hypothetical protein in thiamine biosynthetic operon (66 aa), fasta scores; opt: 138 z-score: 203.8 E(): 0.0005, 30.3% identity in 66 aa overlap and to TR:P96262 (EMBL:Z84724) Mycobacterium tuberculosis hypothetical protein (68 aa) (39.7% identity in 68 aa overlap) 3 2 22 15 [Reporter]SCO2000 (8K15)_PCR/3500 [Gene]SCO2000/3185 NC_003888 SC7H2.14, possible ATP-binding RNA helicase, len: 998 aa; similar to many proposed helicases eg. SW:HRPB_ECOLI ATP-dependent helicase from Escherichia coli (809 aa) fasta scores; opt: 889, z-score: 783.0, E(): 0, (33.6% identity in 998 aa overlap). Contains a repeat region which does not appear in homologues: 8 x GDDRR(R/G)QRATVES. Also contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00037 Myb DNA-binding domain repeat signature 1. 3 2 22 14 [Reporter]SCO7537 (9G15)_PCR/3499 [Gene]SCO7537/3184 NC_003888 SC8G12.13c, aminotransferase, len: 437 aa. Highly similar to several including the bifunctional aminotransferring decarboxylase: 2,2-dialkylglycine decarboxylase (EC 4.1.1.64) from Burkholderia cepacia SW:DGDA_BURCE(EMBL:J05282) (432 aa), fasta scores opt: 1723 z-score: 1953.5 E(): 0 63.7% identity in 422 aa overlap and Mycobacterium leprae SW:GABT_MYCLE(EMBL:U00011) 4-aminobutyrate aminotransferase (EC 2.6.1.19) (446 aa), fasta scores opt: 697 z-score: 792.4 E(): 0 34.0% identity in 418 aa overlap. Contains a Prosite hit to PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site and a Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate. 3 2 22 13 [Reporter]SCO2432 (10C15)_PCR/3498 [Gene]SCO2432/3183 NC_003888 SCC24.03c, possible sugar transporter membrane protein, len: 287 aa; similar to TR:CAB59597 (EMBL:AL132662) Streptomyces coelicolor probable sugar transport inner membrane protein SCF11.20, 299 aa; fasta scores: opt: 673 z-score: 788.9 E(): 0; 35.3% identity in 278 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 3 2 22 12 [Reporter]SCO4581 (11O11)_PCR/3497 [Gene]SCO4581/3182 NC_003888 SCD16A.02c, conserved hypothetical protein, len: 269aa; similar to many hypothetical proteins egs. TR:CAA22232 (EMBL:AL034355) (280 aa) fasta scores; opt: 1028, z-score: 1143.6, E(): 0, (61.5% identity in 260 aa overlap) and TR:CAA22222 (EMBL:AL034355) (305 aa) fasta scores; opt: 546, z-score: 611.4, E(): 1e-26, (40.2% identity in 271 aa overlap) hypothetical proteins from Streptomyces coelicolor. Also similar to SW:YIDA_ECOLI hypothetical protein from Escherichia coli (270 aa) fasta scores; opt: 314, z-score: 356.2, E(): 1.6e-12, (31.0% identity in 268 aa overlap). Contains Pfam match to entry PF00592 DUF3, Domain of Unknown Function 3, score 116.90, E-value 3.9e-31 3 2 22 11 [Reporter]SCO4157 (12K11)_PCR/3496 [Gene]SCO4157/3181 NC_003888 SCD84.24c, probable protease, len: 347 aa; similar to C-terminal region of TR:O06291(EMBL:Z98260) Mycobacterium tuberculosis HtrA, 549 aa; fasta scores: opt: 426 z-score: 370.9 E(): 3.4e-13; 38.4% identity in 328 aa overlap and C-terminal region similar to C-terminal region of SW:HTRA_ECOLI (EMBL:M36536) Escherichia coli protease do precursor HtrA, 474 aa; fasta scores: opt: 329 z-score: 290.0 E(): 1.1e-08; 42.7% identity in 199 aa overlap. Contains 2x Pfam match to entry PF00089 trypsin, Trypsin. Contains possible hydrophobic membrane spanning region 3 2 22 10 [Reporter]SCO3008 (13G11)_PCR/3495 [Gene]SCO3008/3180 NC_003888 SCE33.10c, probable two-component system response regulator, len: 252 aa; similar to TR:AAF10563 (EMBL:AE001951) Deinococcus radiodurans DNA-binding response regulator DR0987, 239 aa; fasta scores: opt: 576 z-score: 658.2 E(): 3.3e-29; 44.0% identity in 218 aa overlap and to various Streptomyces coelicolor putative two-component response regulators, e.g. TR:CAB59507 (EMBL:AL132648) SCI41.37, 223 aa; fasta scores: opt: 622 z-score: 571.0 E(): 2.8e-26; 47.2% identity in 214 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family, and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Contains also possible helix-turn-helix motif at residues 204..225 (+5.13 SD) 4 3 16 11 [Reporter]SCO4804 (12D6)_PCR/5884 [Gene]SCO4804/5359 NC_003888 SCD63A.15, hypothetical protein, len: 815 aa; similar to TR:CAB61596 (EMBL:AL133210) Streptomyces coelicolor hypothetical 135.7 kD protein SCG11A.15, 1320 aa; fasta scores: opt: 340 z-score: 332.5 E(): 4.6e-11; 30.4% identity in 883 aa overlap 4 3 16 10 [Reporter]SCO3038 (13P2)_PCR/5883 [Gene]SCO3038/5358 NC_003888 SCE34.19, hypothetical protein, len: 326 aa; similar to TR:P96819 (EMB:LZ92770) Mycobacterium tuberculosis hypothetical 33.0 kD protein MTCI5.16, 308 aa; fasta scores: opt: 902 z-score: 980.1 E(): 0; 51.2% identity in 287 aa overlap and C-terminal region presents low similarity to SW:3MGA_BACSU (EMBLD14465:) Bacillus subtilis DNA-3-methyladenine glycosylase (EC 3.2.2.21) AlkA, 303 aa; fasta scores: opt: 146 z-score: 166.1 E(): 0.086; 23.6% identity in 208 aa overlap 4 3 16 9 [Reporter]SCO0899 (14L2)_PCR/5882 [Gene]SCO0899/5357 NC_003888 SCM1.32, possible hydrolase, len: 298 aa. Similar to many including: Moraxella sp. SW:DEH1_MORSP (EMBL:D90422:) haloacetate dehalogenase H-1 (EC 3.8.1.3) (294 aa), fasta scores opt: 669 z-score: 782.5 E():0 39.2% identity in 296 aa overlap and Streptomyces coelicolor TR:O86546 (EMBL:AL031350) putative hydrolase SC1F2.09C (309 aa), fasta scores opt: 336 z-score: 396.1 E(): 1e-14 31.1% identity in 305 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 4 3 16 8 [Reporter]SCO0595 (15H2)_PCR/5881 [Gene]SCO0595/5356 NC_003888 SCF55.19, hypothetical protein, len: 197 aa; similar to TR:O05917 (EMBL:Z95210) Mycobacterium tuberculosis hypothetical 26.9 KD protein MTCY21C12.19c, 245 aa; fasta scores: opt: 459 z-score: 543.0 E(): 7.2e-23; 39.3% identity in 201 aa overlap 4 3 16 7 [Reporter]SCO4969 (16D2)_PCR/5880 [Gene]SCO4969/5355 NC_003888 2SCK31.29c, possible regulatory protein, len: 1167 aa; similar in its middle domain to SW:AFSR_STRCO (EMBL:D90155) Streptomyces coelicolor regulatory protein AfsR, 993 aa; fasta scores: opt: 298 z-score: 279.4 E(): 5e-08; 28.9% identity in 806 aa overlap. Contains 5x Pfam matches to entry PF00515 TPR, TPR Domain 4 3 16 6 [Reporter]SCO0353 (16P22)_PCR/5879 [Gene]SCO0353/5354 NC_003888 SCF41.12, possible binding-protein-dependent transport protein, len: 303 aa. Similar to many including: Streptomyces coelicolor TR:CAB41203 (EMBL; AL049661) putative binding protein dependent transport protein SCE134.05c (327 aa), fasta scores opt: 560 z-score: 661.2 E(): 1.8e-29 35.5% identity in 301 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Contains multiple potential membrane-spanning hydrophobic domains. 4 3 16 5 [Reporter]SCO5469 (17L22)_PCR/5878 [Gene]SCO5469/5353 NC_003888 SC2A11.03c, sdaA, probable L-serine dehydratase, len: 455 aa; highly similar to e.g. SDHL_ECOLI L-serine dehydratase 1 (EC 4.2.1.13) (454 aa), fasta scores; opt: 1643 z-score: 1576.6 E(): 0, 55.6% identity in 459 aa overlap 4 3 16 4 [Reporter]SCO0525 (18H22)_PCR/5877 [Gene]SCO0525/5352 NC_003888 SCF11.05, hypothetical protein, len: 271 aa; similar to various hypothetical proteins, e.g. TR:CAB45341 (EMBL:AL079345) Streptomyces coelicolor SCE68.05C hypothetical 33.1 KD protein, 300 aa; fasta scores: opt: 808 z-score: 960.4 E(): 0; 53.2% identity in 250 aa overlap 3 2 22 8 [Reporter]SCO0753 (15O7)_PCR/3493 [Gene]SCO0753/3179 NC_003888 SCF81.12, unknown, len: 71 aa 4 3 16 3 [Reporter]SCP1.241c (19D22)_PCR/5876 [Gene]SCP1.241c/5351 NC_003888 SCP1.241c, mmyT, possible thioesterase, len: 269aa; previously sequenced and annotated as TR:Q9JN88 (EMBL:AJ276673) - note difference in predicted translational start site. Similar to many others eg. SW:P14686 (GRST_BACBR) gramicidin S biosynthesis GrsT protein (EC 3.1.2.-) from Bacillus brevis (256 aa) fasta scores; opt: 289, z-score: 338.3, E(): 2.2e-11, 29.7% identity in 256 aa overlap. Contains Pfam match to entry PF00975 Thioesterase, Thioesterase domain 3 2 22 6 [Reporter]SCO3913 (17G7)_PCR/3491 [Gene]SCO3913/3178 NC_003888 SCH24.35c, hypothetical protein, len: 150 aa; similar to TR:Q54244 (EMBL:D29961) Streptomyces griseus phnO-like regulator for C-P lyase (150 aa), fasta scores; opt: 797 z-score: 972.8 E(): 0, 79.3% identity in 150 aa overlap and to SW:PHNO_ECOLI (EMBL:D90227), phnO, in Escherichia coli alkylphosphonate utilization operon (144 aa) (32.2% identity in 146 aa overlap). Weakly similar to TR:O86792 (EMBL:AL031317) S.coelicolor probable acetyltransferase (177 aa) (32.7% identity in 150 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family, score 80.70, E-value 3.1e-20 4 3 16 2 [Reporter]SCO7480 (20L18)_PCR/5875 [Gene]SCO7480/5350 NC_003888 SCBAC17A6.13, conserved hypothetical protein, len: 207aa: similar to many from streptomycetes eg. TR:Q9L267 (EMBL:AL137778) hypothetical protein SCL2.32 from Streptomyces coelicolor (186 aa) fasta scores; opt: 221, Z-score: 262.6, 31.928% identity (32.515% ungapped) in 166 aa overlap. 3 2 22 5 [Reporter]SCO3703 (18C7)_PCR/3490 [Gene]SCO3703/3177 NC_003888 SCH35.21c, possible substrate binding protein, len: 131aa; similar to many eg. SW:MOP2_CLOPA molybdenum-pterin binding protein from Clostridium pasteurianum (68 aa) fasta scores; opt: 150, z-score: 204.,1 E(): 0.00048, (42.9% identity in 63 aa overlap). Contains probable helix-turn-helix at 5-26aa (+5.01 SD). 3 2 22 4 [Reporter]SCO4043 (19O3)_PCR/3489 [Gene]SCO4043/3176 NC_003888 2SCD60.09c, conserved hypothetical protein, len: 98 aa; similar to TR:O05823 (EMBL:Z95208) Mycobacterium tuberculosis hypothetical 12.0 kDa protein MTCY27.05c, 105 aa; fasta scores: opt: 419 z-score: 582.4 E(): 7e-25; 62.2% identity in 98 aa overlap 3 2 22 3 [Reporter]SCO1371 (20K3)_PCR/3488 [Gene]SCO1371/3175 NC_003888 SC10A9.13, probable oxidoreductase, len: 278 aa; similar to SW:PHZF_PSEFL (EMBL:L48616) Pseudomonas fluorescens phenazine biosynthesis protein PhzF, 278 aa; fasta scores: opt: 279 z-score: 322.8 E(): 1.5e-10; 30.9% identity in 285 aa overlap 3 2 21 22 [Reporter]SCO1090 (1C23)_PCR/3485 [Gene]SCO1090/3174 NC_003888 2SCG4.06, possible phosphodiesterase, len: 227 aa; similar to TR:Q9RKX8 (EMBL:AL133213) Streptomyces coelicolor putative phosphodiesterase SC6D7.20c, 255 aa; fasta scores: opt: 287 z-score: 341.1 E(): 1.6e-11; 33.9% identity in 230 aa overlap and to SW:GLPQ_BACSU (EMBL:Z26522) Bacillus subtilis glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46) GlpQ, 293 aa; fasta scores: opt: 268 z-score: 318.3 E(): 3e-10; 30.8% identity in 247 aa overlap 3 2 21 21 [Reporter]SCO2610 (2O19)_PCR/3484 [Gene]SCO2610/3173 NC_003888 SCC88.21c, mreC, rod shape-determining protein, len: 341 aa; identical to previously sequenced TR:O33620 (EMBL:Y14206) Streptomyces coelicolor MreC protein, 366 aa and similar to SW:MREC_ECOLI (EMBL:M31792) Escherichia coli rod shape-determining protein MreC, 367 aa; fasta scores: opt: 258 z-score: 276.5 E(): 5.5e-08; 24.4% identity in 336 aa overlap. Contains possible coiled-coil region and possible N-terminal region signal peptide sequence 3 2 21 20 [Reporter]SCO6429 (3K19)_PCR/3483 [Gene]SCO6429/3172 NC_003888 SC1A6.18, unknown, len: 462 aa; some similarity to a hypothetical proteins from Streptomyces hygroscopicus TR:Q54310 (EMBL:X86780) ORFE from gene cluster for polyketide immunosuppressant rapamycin (465 aa), fatsa scores; opt: 306 z-score: 225.9 E(): 1.8e-05, 28.3% identity in 441 aa overlap 3 2 21 19 [Reporter]SCO5617 (4G19)_PCR/3482 [Gene]SCO5617/3171 NC_003888 SC2E1.34, unknown prophage gene, len: 138 aa 3 2 21 18 [Reporter]SCO7100 (5C19)_PCR/3481 [Gene]SCO7100/3170 NC_003888 SC4B10.01, probable oxidoreductase, len: 260 aa; similar to TR:Q9RK83 (EMBL:AL132662) Streptomyces coelicolor possible oxidoreductase SCF11.06, 277 aa; fasta scores: opt: 208 z-score: 258.9 E(): 5.9e-07; 25.5% identity in 243 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 4 3 15 22 [Reporter]SCO1209 (1L14)_PCR/5873 [Gene]SCO1209/5349 NC_003888 2SCG58.09c, probable acyl-CoA dehydrogenase, len: 408 aa; similar to SW:ACDA_BACSU (EMBL:Z49782) Bacillus subtilis acyl-CoA dehydrogenase (EC 1.3.99.-) AcdA, 379 aa; fasta scores: opt: 512 z-score: 587.6 E(): 2.9e-25; 41.3% identity in 400 aa overlap. Contains 2x Pfam matches to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase and match to Prosite entry PS00073 Acyl-CoA dehydrogenases signature 2 4 3 15 21 [Reporter]SCO3136 (2H14)_PCR/5872 [Gene]SCO3136/5348 NC_003888 SCE66.15c, galK, galactokinase (EC 2.7.1.6), len: 387aa; strongly similar to many eg. SW:P13227 (GAL1_STRLI) galactokinase from Streptomyces lividans (397 aa) fasta scores; opt: 2131, z-score: 2454.6, E(): 0, 88.8% identity in 383 aa overlap. Contains Pfam match to entry PF00288 GHMP_kinases, GHMP kinases putative ATP-binding proteins, Prosite match to PS00627 GHMP kinases putative ATP-binding domain and Prosite match to PS00106 Galactokinase signature. 4 3 15 20 [Reporter]SCO2220 (3D14)_PCR/5871 [Gene]SCO2220/5347 NC_003888 SC10B7.15, unknown, len: 643 aa 4 3 15 19 [Reporter]SCO7015 (4P10)_PCR/5870 [Gene]SCO7015/5346 NC_003888 SC1H10.04c, possible secreted glycosyl hydrolase, len: 1433 aa. Identical to Streptomyces lividans TR:P75020(EMBL:U22894) chitinase homolog found within the 4.7 kb amplifiable unit of the AUD1 element (1433 aa), fasta scores opt: 9322 z-score: 9526.2 E():0 99.5% identity in 1433 aa overlap. C-terminal similar to TR:BAB62749 (EMBL:AB055506) mycodextranase from Streptomyces sp. J-13-3 fasta scores; opt: 865, Z-score: 887.6, 32.953% identity (36.861% ungapped) in 613 aa overlap. Also weakly similar, in parts, to Bacillus circulans SW:CHI1_BACCI(EMBL:M57601) chitinase A1 precursor (EC 3.2.1.14) (699 aa), fasta scores opt: 497 z-score: 507.9 E(): 7.2e-21 47.3% identity in 182 aa overlap. Contains a possible N-terminal signal sequence and 2x Pfam matches to entries PF00041 fn3, Fibronectin type III domain. 4 3 15 18 [Reporter]SCO2093 (5L10)_PCR/5869 [Gene]SCO2093/5345 NC_003888 SC4A10.26, conserved hypothetical protein, len: 167 aa; unknown function, similar to other hypothetical proteins e.g. SW:Y023_MYCTU (EMBL:Z73419) Mycobacterium tuberculosis hypothetical protein (163 aa), fasta scores; opt: 567 z-score: 691.9 E(): 3.2e-31, 53.4% identity in 163 aa overlap. Very weak similarity to the C-terminus of many carbonic anhydrases e.g. TR:P93109 (EMBL:U80805) Chlamydomonas reinhardtii beta-carbonic anhydrase (267 aa) (24.3% identity in 181 aa overlap) 4 3 15 17 [Reporter]SCO5794 (6H10)_PCR/5868 [Gene]SCO5794/5344 NC_003888 SC4H2.15, possible kinase/phosphohydrolase, len: 725; similar to many RelA and SpoT genes e.g. RELA_STRCO P52560 GTP pyrophosphokinase (ec 2.7.6.5) (847 aa), fasta scores; opt: 1049 z-score: 1312.4 E(): 0, 39.5% identity in 792 aa overlap and SPOT_ECOLI P17580 guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (702 aa), fasta scores; opt:1040 z-score: 867.0 E(): 0, 33.8% identity in 695 aa overlap 4 3 15 16 [Reporter]SCO5687 (7D10)_PCR/5867 [Gene]SCO5687/5343 NC_003888 SC5H4.11, probable transport system integral membrane protein, len: 346 aa; similar to SW:LPLB_BACSU (EMBL:L19163) Bacillus subtilis LplB protein, 318 aa; fasta scores: opt: 814 z-score: 950.7 E(): 0; 38.2% identity in 309 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 4 3 15 15 [Reporter]SCO2471 (8P6)_PCR/5866 [Gene]SCO2471/5342 NC_003888 SC7A8.10c, possible secreted protein, len: 274 aa; similar to various hypothetical proteins, e.g. TR:P95216 (EMBL:Z86089) Mycobacterium tuberculosis hypothetical 26.2 kD protein, 247 aa; fasta scores: opt:413 z-score: 450.1 E(): 1.1e-17; 34.5% identity in 232 aa overlap. Contains possible N-terminal region signal peptide sequence 3 2 21 17 [Reporter]SCO2186 (6O15)_PCR/3480 [Gene]SCO2186/3169 NC_003888 SC5F7.15, unknown, len: 234aa; 4 3 15 14 [Reporter]SCO7836 (9L6)_PCR/5865 [Gene]SCO7836/5341 NC_003888 SC8E7.33, unknown, len: 269 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1Y3(EMBL:AL109973) hypothetical protein, SCJ30.06c (269 aa). Note the large overlap with the adjoining CDS. 3 2 21 16 [Reporter]SCO1408 (7K15)_PCR/3479 [Gene]SCO1408/3168 NC_003888 SC6D7.31, possible membrane protein, len: 293 aa. Contains a possible membrane spanning hydrophobic domain. 4 3 15 13 [Reporter]SCO6212 (10H6)_PCR/5864 [Gene]SCO6212/5340 NC_003888 SC9G1.02, possible permease, len: 471 aa; similar to SW:PBUX_BACSU (EMBL:X83878) Bacillus subtilis xanthine permease PubX, 438 aa; fasta scores: opt: 1060 z-score: 1156.8 E(): 0; 39.2% identity in 413 aa overlap to SW:URAA_ECOLI (EMBL:X73586) Escherichia coli uracil permease (uracil transporter) UraA, 429 aa; fasta scores: opt: 505 z-score: 554.2 E(): 1.7e-23; 28.9% identity in 422 aa overlap and to Streptomyces coelicolor St9G1.04, 462 aa; fasta scores: opt: 1668 z-score: 1511.0 E(): 0; 57.0% identity in 435 aa overlap. Contains match to Pfam entry Pfam match to entry PF00860 xan_ur_permease, Xanthine/uracil permeases family and match to Prosite entry PS01116 Xanthine/uracil permeases family signature 3 2 21 15 [Reporter]SCO5233 (8G15)_PCR/3478 [Gene]SCO5233/3167 NC_003888 SC7E4.30, possible sugar transporter integral membrane protein, len: 328 aa; similar to TR:Q9RK78 (EMBL:AL132662) Streptomyces coelicolor putative sugar transporter integral membrane protein SCF11.12, 302 aa; fasta scores: opt: 907 z-score: 1004.1 E(): 0; 46.6% identity in 298 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and possible hydrophobic membrane spanning regions 3 2 21 14 [Reporter]SCO7533 (9C15)_PCR/3477 [Gene]SCO7533/3166 NC_003888 SC8G12.09, two-component system response regulator protein, len: 233 aa. Highly similar to many putative response regulators including: Amycolatopsis mediterranei TR:CAB82846(EMBL:AJ277082) putative response regulator (229 aa), fasta scores opt: 937 z-score: 1114.4 E(): 0 61.9% identity in 231 aa overlap and Streptomyces coelicolor TR:Q9ZEP4(EMBL:AJ131213) response regulator, SCE94.09 (234 aa), fasta scores opt: 665 z-score: 793.6 E():0 45.5% identity in 231 aa overlap. Contains a Pfam match to entry PF00072 response_reg, Response regulator receiver domain and a Pfam match to entry PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal. 3 2 21 13 [Reporter]SCO2582 (10O11)_PCR/3476 [Gene]SCO2582/3165 NC_003888 SCC123.20, unknown, len: 402 aa. Similar to Mycobacterium tuberculosis TR:O53978(EMBL:AL022073) hypothetical 38.3 KD protein (348 aa), fasta scores opt: 1044 z-score: 981.9 E():0 49.5% identity in 319 aa overlap and Synechocystis sp. (strain PCC 6803) TR:P73529(EMBL:D90907) hypothetical 34.0 KD protein (303 aa), fasta scores opt: 568 z-score: 540.3 E(): 1.1e-22 37.5% identity in 269 aa overlap. 3 2 21 12 [Reporter]SCO4338 (11K11)_PCR/3475 [Gene]SCO4338/3164 NC_003888 SCD12A.21, hypothetical protein, len: 190 aa; similar to TR:Q45633 (EMBL:L13418) Bacillus stearothermophilus maltose permease MalA, 314 aa; fasta scores: opt: 207 z-score: 246.2 E(): 3e-06; 31.3% identity in 160 aa overlap. Contains match to Prosite entry PS00216 Sugar transport proteins signature 1 3 2 21 11 [Reporter]SCO4179 (12G11)_PCR/3474 [Gene]SCO4179/3163 NC_003888 SCD66.16, hypothetical protein, len: 191 aa; similar to TR:O53827 (EMBL:AL022004) Mycobacterium tuberculosis hypothetical 23.9 kD protein MTV043.05c, 226 aa; fasta scores: opt: 320 z-score: 391.4 E(): 2.5e-14; 34.9% identity in 169 aa overlap 3 2 21 9 [Reporter]SCO0543 (14O7)_PCR/3472 [Gene]SCO0543/3162 NC_003888 SCF11.23, possible peptidase, len: 412 aa; similar to various hypothetical proteins, e.g. TR:CAB39706 (EMBL:AL049485) Streptomyces coelicolor putative secreted peptidase, 312 aa; fasta scores: opt: 307 z-score: 329.3 E(): 5.7e-11; 34.3% identity in 201 aa overlap. Contains Pfam match to entry PF01551 peptidase_M37, peptidase family M23/M37. Contains possible hydrophobic membrane spanning region 3 2 21 8 [Reporter]SCO0442 (15K7)_PCR/3471 [Gene]SCO0442/3161 NC_003888 SCF51A.20, unknown, len: 252 aa. Similar to a number of hypothetical proteins e.g. Escherichia coli SW:YBGJ_ECOLI (EMBL; AE000174) hypothetical 23.9 KD protein in phrB-nei intergenic region (218 aa), fasta scores: opt: 477 z-score: 492.1 E(): 4.7e-20 43.5% identity in 214 aa overlap 3 2 21 7 [Reporter]SCO5041 (16G7)_PCR/3470 [Gene]SCO5041/3160 NC_003888 SCK7.14, hypothetical protein, len: 236 aa; similar to TR:O83033 (EMBL:AB016934) Streptomyces wedmorensis FomD protein, 209 aa; fasta scores: opt: 282 z-score: 320.1 E(): 2.3e-10; 34.5% identity in 197 aa overlap 4 3 15 12 [Reporter]SCO2286 (11D6)_PCR/5863 [Gene]SCO2286/5339 NC_003888 SCC75A.32c, possible alkaline phosphatase, len: 558 aa. Highly similar to alkaline phosphatases from Bacillus subtilis SW:PPBD_BACSU (EMBL:U49060) alkaline phosphatase D precursor (EC 3.1.3.1) (556 aa), fasta scores opt: 1652 z-score: 1864.2 E():0 49.6% identity in 488 aa overlap. Also similar to several from Streptomyces coelicolor: EMBL:AL096837 putative alkaline phosphatase SCF43A.18 (553 aa), fasta scores opt: 2452 z-score: 2273.5 E():0 65.0% identity in 552 aa overlap and TR:CAB51460 (EMBL:AL096884) putative secreted alkaline phosphatase (551 aa), fasta scores opt: 928 z-score: 1047.4 E(): 0 38.5% identity in 556 aa overlap. Contains possible hydrophobic membrane spanning region 4 3 15 11 [Reporter]SCO4123 (12P2)_PCR/5862 [Gene]SCO4123/5338 NC_003888 SCD72A.09c, possible two-component system response regulator, len: 222aa; similar to many eg. TR:O86632 (EMBL:AL031155) possible two-component regulator from Streptomyces coelicolor (222 aa) fasta scores; opt: 973, z-score: 1128.5, E(): 0, 69.1% identity in 220 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain, Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and Prosite match to PS00622 Bacterial regulatory proteins, luxR family signature. 4 3 15 10 [Reporter]SCO3033 (13L2)_PCR/5861 [Gene]SCO3033/5337 NC_003888 SCE34.14c, possible integral membrane regulatory protein, len: 1268 aa; N-terminal region identical to previously sequenced TR:Q53964 (EMBL:X62287) Streptomyces coelicolor regulatory protein WhiB, 914 aa and highly similar to TR:Q53785 (EMBL:L22864) Streptomyces aureofaciens transmembrane protein WhiB2, 1219 aa; fasta scores: opt: 4895 z-score: 4433.4 E(): 0; 62.0% identity in 1267 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 15 9 [Reporter]SCO2901 (14H2)_PCR/5860 [Gene]SCO2901/5336 NC_003888 SCE19A.01, hypothetical protein, partial CDS, len: >31 aa; unknown function, similar to members of the alkyl hydroperoxide reductase C/thiol-specific antioxidant family e.g. TR:O53226 (EMBL:AL021185), bcp, Mycobacterium tuberculosis bacterioferritin comigratory protein (157 aa), fasta scores; opt: 124 z-score: 238.1 E(): 6.2e-06, 63.3% identity in 30 aa overlap,SCE6.38, unknown, len: 155 aa. Highly similar to many bacterioferritin co migratory proteins which have no known function e.g. Escherichia coli SW:BCP_ECOLI(EMBL:M63654) bacterioferritin co migratory protein, Bcp (156 aa), fasta scores opt: 516 z-score: 621.8 E(): 3.3e-27 51.1% identity in 141 aa overlap. Overlaps and extends into CDS SCE19A.01 on the adjoining cosmid. Contains a Pfam match to entry PF00578 AhpC-TSA, AhpC/TSA family. 4 3 15 8 [Reporter]SCO3167 (15D2)_PCR/5859 [Gene]SCO3167/5335 NC_003888 SCE87.18c, possible tetR-family transcriptional regulator, len: 199 aa. Similar to many including: Propionibacterium freudenreichii TR:P72185(EMBL:U51164) repressor protein (HemR protein) (259 aa), fasta scores opt: 222 z-score: 276.8 E(): 4.8e-08 30.6% identity in 196 aa overlap and Streptomyces coelicolor TR:CAB45568(EMBL:AL079355) putative transcriptional regulator (fragment) SC4C6.04 (204 aa), fasta scores opt: 539 z-score: 660.1 E(): 2.2e-29 42.9% identity in 191 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. 4 3 15 7 [Reporter]SCO0362 (15P22)_PCR/5858 [Gene]SCO0362/5334 NC_003888 SCF41.21, possible racemase, len: 403 aa. Weakly similar to Eukaryotic and Prokaryotic racemases e.g. Homo sapiens (Human) TR:O43673 (EMBL; AF047020) alpha-methylacyl-CoA racemase (EC 5.1.99.4) (382 aa), fasta scores opt: 466 z-score: 544.7 E(): 5.5e-23 31.1% identity in 392 aa overlap and Streptomyces coelicolor TR:CAB40680 (EMBL; AL049587) putative racemase SC5F2A.13 (398 aa), fasta scores opt: 508 z-score: 593.2 E(): 1.1e-25 34.5% identity in 383 aa overlap 4 3 15 6 [Reporter]SCO5057 (16L22)_PCR/5857 [Gene]SCO5057/5333 NC_003888 SCK7.30c, possible amino acid permease, len: 460 aa; similar to SW:MMUP_ECOLI (EMBL:D83536) Escherichia coli probable S-methylmethionine permease MmuP, 475 aa; fasta scores: opt: 345 z-score: 390.5 E(): 2.8e-14; 23.3% identity in 408 aa overlap and to TR:P74848 (EMBL:X99945) Salmonella typhimurium amino acid permease YeeF like protein, 448 aa; fasta scores: opt: 705 z-score: 792.4 E(): 0; 32.0% identity in 416 aa overlap. Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease and possible hydrophobic membrane spanning regions 4 3 15 5 [Reporter]SCO4864 (17H22)_PCR/5856 [Gene]SCO4864/5332 NC_003888 SCK20.05, possible ECF sigma factor, len: 225 aa; similar to TR:Q9RIT1 (EMBL:AJ010584) Streptomyces coelicolor ECF sigma factor, 264 aa; fasta scores: opt: 296 z-score: 342.7 E(): 1.5e-11; 34.0% identity in 212 aa overlap and to Streptomyces coelicolor putative ECF sigma factor SCK20.07, 220 aa; fasta scores: opt: 671 z-score: 656.4 E(): 6.9e-31; 59.5% identity in 185 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 3 2 21 6 [Reporter]SCO3725 (17C7)_PCR/3469 [Gene]SCO3725/3159 NC_003888 SCH22A.03c, possible membrane protein, len: 429 aa. similar to TR:Q9F388 (EMBL:AL450165) from Streptomyces coelicolor. Contains possible hydrophobic membrane spanning regions 4 3 15 4 [Reporter]SCO1079 (18D22)_PCR/5855 [Gene]SCO1079/5331 NC_003888 SCG22.25, hypothetical protein, len: 277 aa; similar to TR:CAB88921 (EMBL:AL353862) Streptomyces coelicolor putative oxidoreductase SCE34.18, 443 aa; fasta scores: opt: 133 z-score: 155.6 E(): 0.34; 28.2% identity in 287 aa overlap 3 2 21 5 [Reporter]SCO3870 (18O3)_PCR/3468 [Gene]SCO3870/3158 NC_003888 SCH18.07c, possible integral membrane protein, len: 66 aa. Contains possible hydrophobic membrane spanning regions 4 3 15 3 [Reporter]SCP1.313 (19P18)_PCR/5854 [Gene]SCP1.313/5330 NC_003888 SCP1.313, unknown, len: 411aa; weakly similar to many eg. TR:Q9L195 (EMBL:AL158057) hypothetical protein from Streptomyces coelicolor (437 aa) fasta scores; opt: 476, z-score: 570.6, E(): 2.6e-24, 32.7% identity in 327 aa overlap. 3 2 21 4 [Reporter]SCO1308 (19K3)_PCR/3467 [Gene]SCO1308/3157 NC_003888 SCBAC36F5.19, probable hydrolase, len: 407 aa; similar to SW:PHHY_PSEFL (EMBL:X68438) Pseudomonas fluorescens P-hydroxybenzoate hydroxylase (EC 1.14.13.2) PobA, 394 aa; fasta scores: opt: 907 Z-score: 1007.6 bits: 195.3 E(): 1.6e-48; 39.018% identity in 387 aa overlap. Contains Pfam matches to entries PF01494 FAD_binding_3, FAD binding domain and PF01360 Monooxygenase, Monooxygenase 3 2 21 3 [Reporter]SCO6112 (20G3)_PCR/3466 [Gene]SCO6112/3156 NC_003888 SCBAC1A6.36c, probable oligopeptide ABC transporter integral membrane protein, len: 297 aa; similar to SW:APPC_BACSU (EMBL:U20909) Bacillus subtilis oligopeptide transport system permease protein AppC, 303 aa; fasta scores: opt: 725 z-score: 842.9 E(): 0; 39.4% identity in 287 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Also contains possible hydrophobic membrane spanning regions 3 2 21 2 [Reporter]SCO5094 (20O23)_PCR/3465 [Gene]SCO5094/3155 NC_003888 SCBAC28G1.20c, ORF8, putative methyltransferase, len: 208 aa; identical to previously sequenced TR:Q9X9V1 (EMBL:Y18817) Streptomyces coelicolor A3(2) putative methyltransferase ORF8, 208 aa 3 2 20 22 [Reporter]SCO1351 (1O19)_PCR/3463 [Gene]SCO1351/3154 NC_003888 2SCG61.33, possible large transcriptional regulator, len: 912 aa; similar to many from actinomycetes e.g. TR:Q9Z573 (EMBL:AL035569) putative regulatory protein from Streptomyces coelicolor (1091 aa) fasta scores; opt: 233, z-score: 243.0, E(): 4.6e-06, 26.7% identity in 899 aa overlap. C-terminal region contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, Prosite match to PS00622 Bacterial regulatory proteins, luxR family signature and a helix-turn-helix motif (residue 867-888). N-terminal region contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 2 20 21 [Reporter]SCO3181 (2K19)_PCR/3462 [Gene]SCO3181/3153 NC_003888 SCE87.32c, moeA, probable molybdopterin biosynthesis protein. len: 424 aa. Highly similar to many e.g. Mycobacterium tuberculosis SW:MOEA_MYCTU (EMBL:Z94752) molybdopterin biosynthesis protein (426 aa), fasta scores opt: 795 z-score: 812.4 E():0 39.3% identity in 438 aa overlap. Contains a Pfam match to entry PF00994 MoCF_biosynth, Molybdenum cofactor biosynthesis protein. 3 2 20 20 [Reporter]SCO7661 (3G19)_PCR/3461 [Gene]SCO7661/3152 NC_003888 SC10F4.34, possible secreted protein, len: 212 aa; similar to TR:Q9XBC6 (EMBL:AL078635) Amycolatopsis orientalis hypothetical 22.9 kDa protein CZA382.25, 205 aa; fasta scores: opt: 324 z-score: 399.0 E(): 1.1e-14; 35.8% identity in 201 aa overlap. Contains possible N-terminal region signal peptide sequence 3 2 20 19 [Reporter]SCO5613 (4C19)_PCR/3460 [Gene]SCO5613/3151 NC_003888 SC2E1.30, unknown prophage gene, len: 183 aa 3 2 20 18 [Reporter]SCO7686 (5O15)_PCR/3459 [Gene]SCO7686/3150 NC_003888 SC4C2.21, probable cytochrome P450, len: 411 aa; similar to SW:CPXE_STRGO (EMBL:M32238) Streptomyces griseolus cytochrome P450-SU1 SuaC,405 aa; fasta scores: opt: 1113 z-score: 1179.8 E(): 0; 45.5% identity in 407 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450 and match to Prosite entry PS00086 Cytochrome P450 cysteine heme-iron ligand signature 4 3 15 2 [Reporter]SCO3970 (20H18)_PCR/5853 [Gene]SCO3970/5329 NC_003888 SCBAC25E3.07c, Xaa-Pro aminopeptidase, len: 491aa: identical to SW:Q05813 (AMP1_STRLI) aminopeptidase P I from Streptomyces lividans (490 aa) fasta scores; opt: 3229, Z-score: 3571.1, 100.000% identity (100.000% ungapped) in 490 aa overlap. Similar to many others eg. SW:Q60394 (AMP2_STRCO) aminopeptidase P II from Streptomyces coelicolor (470 aa) fasta scores; opt: 2060, Z-score: 2279.7, 66.383% identity (67.241% ungapped) in 470 aa overlap. Contains Pfam match to entry PF00557 Peptidase_M24, metallopeptidase family M24 and Prosite match to PS00491 Aminopeptidase P and proline dipeptidase signature. 4 3 14 22 [Reporter]SCO0992 (1H14)_PCR/5851 [Gene]SCO0992/5328 NC_003888 2SCG2.05, possible cysteine synthase, len: 340 aa; similar to SW:CYSM_ALCEU (EMBL:X97499) Alcaligenes eutrophus cysteine synthase (EC 4.2.99.8) CysM, 339 aa; fasta scores: opt: 283 z-score: 331.0 E(): 6.5e-11; 30.3% identity in 277 aa overlap. Contains Pfam match to entry PF00291 PALP, Pyridoxal-phosphate dependent enzyme. Also contains TTA leucine codon (115), possible target for bldA regulation 4 3 14 21 [Reporter]SCO7035 (2D14)_PCR/5850 [Gene]SCO7035/5327 NC_003888 SC2C3.02, gabD, succinate-semialdehyde dehydrogenase (fragment), len: >422 aa; highly similar to SW:GABD_ECOLI (EMBL:M88334) Escherichia coli succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.16) GabD, 482 aa; fasta scores: opt: 1329 z-score: 1466.8 E(): 0; 48.9% identity in 415 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family and match to Prosite entry PS00687 Aldehyde dehydrogenases glutamic acid active site,SC4G1.01, gabD, succinate-semialdehyde dehydrogenase (fragment), len: >90 aa; similar to SW:GABD_ECOLI (EMBL:M88334) Escherichia coli succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.16) GabD, 482 aa; fasta scores: opt: 320 z-score: 405.5 E(): 4.1e-15; 50.6% identity in 87 aa overlap 4 3 14 20 [Reporter]SCO5911 (3P10)_PCR/5849 [Gene]SCO5911/5326 NC_003888 SC10A5.16c, possible oligopeptide binding protein, len: 521; weak similarity to many eg. OPPA_SALTY P06202 periplasmic oligopeptide-binding protein (542 aa), fasta scores; opt: 343 z-score: 306.2 E(): 7.4e-10, 25.0% i dentity in 537 aa overlap. Contains N-terminal signal seque nce 4 3 14 18 [Reporter]SCO5448 (5H10)_PCR/5847 [Gene]SCO5448/5325 NC_003888 SC3D11.05c, probable ABC transporter, len: 583aa; similar to many eg. TR:Q54204 (EMBL:X89010) from the 5'-hydroxystreptomycin production/transport operon of Streptomyces glaucescens (591 aa) fasta scores; opt: 1687, z-score: 1814.2, E(): 0, 49.3% identity in 564 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, Pfam match to entry PF00664 ABC_membrane, ABC transporter transmembrane region and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 3 14 17 [Reporter]SCO5788 (6D10)_PCR/5846 [Gene]SCO5788/5324 NC_003888 SC4H2.09, unknown, len: 237 aa; similar to a hypothetical protein from Mycobacterium tuberculosis and M. leprae, TTR:O33233 (EMBL:Z98209) MTCY154.08C (231 aa), fasta scores; opt: 306 z-score: 320.4 E(): 1.3e-10, 33.2% identity in 241 aa overlap 4 3 14 16 [Reporter]SCO6757 (7P6)_PCR/5845 [Gene]SCO6757/5323 NC_003888 SC6A5.06, probable ABC transporter membrane component, len: 309aa; similar to many eg. TR:O66230 (EMBL:AB010150) ABC transporter membrane component from Escherichia coli (264 aa) fasta scores; opt: 346, z-score: 414.3, E(): 8.9e-16, (27.3% identity in 267 aa overlap). Contains Pfam match to entry PF01061 ABC2_membrane, ABC-2 type transporter, score 109.60, E-value 6.1e-29 and Prosite match to PS00211 ABC transporters family signature. Also contains probable membrane spanning hydrophobic regions. 4 3 14 15 [Reporter]SCO2466 (8L6)_PCR/5844 [Gene]SCO2466/5322 NC_003888 SC7A8.05, hypothetical protein, len: 201 aa; identical to C-terminus of previously sequenced TR:CAB51553 (EMBL:AJ244019) Streptomyces coelicolor A3(2) hypothetical 14.6 kD protein, 132 aa 3 2 20 17 [Reporter]SCO2190 (6K15)_PCR/3458 [Gene]SCO2190/3149 NC_003888 SC5F7.11, unknown, len: 369aa; similar to SW:Y00R_MYCTU hypothetical protein from Mycobacterium tuberculosis (301 aa) fasta scores; opt: 885, z-score: 925.1, E(): 0, (46.9% identity in 290 aa overlap). 4 3 14 14 [Reporter]SCO7735 (9H6)_PCR/5843 [Gene]SCO7735/5321 NC_003888 SC8D11.26, unknown, len: 70 aa 3 2 20 16 [Reporter]SCO6172 (7G15)_PCR/3457 [Gene]SCO6172/3148 NC_003888 SC6C5.08, probable oxidoreductase subunit, len: 828aa; similar to many eg. TR:O32144 (EMBL:Z99120) Yurc protein from Bacillus subtilis (745 aa) fasta scores; opt: 626, z-score: 1140.2, E(): 0, (33.1% identity in 773 aa overlap) and SW:MOP_DESGI aldehyde oxidoreductase Desulfovibrio gigas (907 aa) fasta scores; opt: 538, z-score: 893.1, E(): 0, (29.5% identity in 791 aa overlap). Contains Pfam match to entry PF01315 Ald_Xan_dh_C, Aldehyde oxidase and xanthine dehydrogenase, C terminus, score 497.50, E-value 1e-145. 4 3 14 12 [Reporter]SCO2360 (11P2)_PCR/5841 [Gene]SCO2360/5320 NC_003888 SC8A.18, putative membrane protein, len: 293 aa; region similar to many of undefined function eg. SW:Q48630 (APL_LACLA) alkaline phosphatase like protein from Lactococcus lactis (242 aa) fasta scores; opt: 554, z-score: 549.3, E(): 3.9e-23, 46.4% identity in 194 aa overlap. Contains Pfam match to entry PF00597 DedA, DedA family. Contains possible hydrophobic membrane spanning regions 3 2 20 15 [Reporter]SCO5229 (8C15)_PCR/3456 [Gene]SCO5229/3147 NC_003888 SC7E4.26c, probable permease, len: 4532 aa; similar to TR:Q47552 (EMBL:L01152) Escherichia coli proline permease (fragment) PutP, 489 aa; fasta scores: opt: 333 z-score: 378.7 E(): 1.3e-13; 23.8% identity in 495 aa overlap. Contains Pfam match to entry PF00474 SSF, Sodium:solute symporter family. Contains also possible hydrophobic membrane spanning regions 3 2 20 14 [Reporter]SCO5400 (9O11)_PCR/3455 [Gene]SCO5400/3146 NC_003888 SC8F4.04, possible transport system kinase, len: 318 aa; similar to SW:P27254 (ARGK_ECOLI) LAO/AO transport system kinase from Escherichia coli. Thought to phosphorylate the LAO/AO periplasmic binding proteins. (331 aa) fasta scores; opt: 562, z-score: 600.4, E(): 4.9e-26,35.1% identity in 271 aa overlap. 3 2 20 13 [Reporter]SCO2578 (10K11)_PCR/3454 [Gene]SCO2578/3145 NC_003888 SCC123.16c, possible membrane protein, len: 592 aa. Contains a possible membrane spanning hydrophobic domain. The protein is rich in the amino acid Gln and has a notable repeat region with 6x QQ(G/Y) between residues 53..84. 3 2 20 12 [Reporter]SCO2600 (11G11)_PCR/3453 [Gene]SCO2600/3144 NC_003888 SCC88.11c, hypothetical protein, len: 258 aa; similar to TR:P73260 (EMBL:D90905) Synechocystis sp. hypothetical 97.4 kD protein, 865 aa; fasta scores: opt: 290 z-score: 317.3 E(): 2.9e-10; 31.3% identity in 179 aa overlap 3 2 20 11 [Reporter]SCO4174 (12C11)_PCR/3452 [Gene]SCO4174/3143 NC_003888 SCD66.11c, possible integral membrane protein, len: 83 aa. Contains possible hydrophobic membrane spanning region 3 2 20 10 [Reporter]SCO3284 (13O7)_PCR/3451 [Gene]SCO3284/3142 NC_003888 SCE15.01, partial CDS, conserved hypothetical protein, len: >436aa; similar to C-terminal region of TR:CAA22732 (EMBL:AL035161) another conserved hypothetical protein from Streptomyces coelicolor (860 aa) fasta scores; opt: 922, z-score: 1011.5, E(): 0, (39.5% identity in 425 aa overlap).,SCE39.34, unknown, len: >307aa; similar to TR:E1370406 (EMBL:AL035161) conserved hypothetical protein from Streptomyces coelicolor (860 aa) fasta scores; opt: 254, z-score: 277.0, E(): 4e-08, (29.2% identity in 277 aa overlap) TR:O86875 (EMBL:U22894) hypothetical protein from Streptomyces lividans (547 aa) fasta scores; opt: 226, z-score: 249.7, E(): 1.3e-06, (33.0% identity in 197 aa overlap). 3 2 20 9 [Reporter]SCO0363 (14K7)_PCR/3450 [Gene]SCO0363/3141 NC_003888 SCF41.22, possible integral membrane protein, len: 369 aa. Contains possible membrane spanning hydrophobic domains. 3 2 20 8 [Reporter]SCO5599 (15G7)_PCR/3449 [Gene]SCO5599/3140 NC_003888 SC2E1.16, sip4, probable signal peptidase I, len: 258 aa; similar to many e.g. LEPS_BACSU signal peptidase I (EC 3.4.21.89) (184 aa), fasta scores; opt: 258 z-score: 337.6 E(): 1.4e-11, 32.2% identity in 177 aa overlap. Contains Pfam match to entry signal_pept_I PF00461, Signal peptidases I, score 49.57 and probable transmembrane domain. Also similar to upstream genes sip1 (E(): 8.8e-10, 36.5% identity in 219 aa overlap), sip2 (E(): 3.8e-13, 35.9% identity in 223 aa overlap) and sip3 (E(): 8.1e-15, 35.4% identity in 237 aa overlap). Contains possible hydrophobic membrane spanning regions 4 3 14 11 [Reporter]SCO4119 (12L2)_PCR/5840 [Gene]SCO4119/5319 NC_003888 SCD72A.05, possible NADH dehydrogenase, len: 442aa; similar to many eg. TR:Q9ZC17 (EMBL:AL033505) putative dehydrogenase from Streptomyces coelicolor SC1E6.05 (629 aa) fasta scores; opt: 893, z-score: 1049.2, E(): 0, 38.5% identity in 452 aa overlap and SW:P00393 (DHNA_ECOLI) NADH dehydrogenase Ndh (EC 1.6.99.3) from Escherichia coli (433 aa) fasta scores; opt: 404, z-score: 477.0, E(): 4.2e-19, 27.0% identity in 389 aa overlap. 4 3 14 10 [Reporter]SCO3186 (13H2)_PCR/5839 [Gene]SCO3186/5318 NC_003888 SCE22.03, possible ABC transporter integral membrane protein, len: 421 aa; similar to TR:O50532 (EMBL:AL009204) Streptomyces coelicolor hypothetical 41.1 kD protein SC9B10.25c, 393 aa; fasta scores: opt: 721 z-score: 787.1 E(): 0; 34.7% identity in 363 aa overlap. Contains match to Prosite entry PS00211 ABC transporters family signature and possible hydrophobic membrane spanning regions 4 3 14 9 [Reporter]SCO1609 (14D2)_PCR/5838 [Gene]SCO1609/5317 NC_003888 SCI35.31, unknown, len: 402 aa; similar to a hypothetical protein from M. tuberculosis TR:O06802 (EMBL:Z95890) MTCY28.35 (414 aa), fasta scores; opt: 1341 z-score: 1649.4 E(): 0, 53.8% identity in 398 aa overlap 4 3 14 8 [Reporter]SCO0836 (14P22)_PCR/5837 [Gene]SCO0836/5316 NC_003888 SCF43A.26c, conserved possible membrane protein, len: 333 aa; unknown function, similar to (or to part of) many hypothetical proteins of different sizes e.g. TR:Q55987 (EMBL:D64005) Synechocystis sp. hypothetical protein (617 aa), fasta scores; opt: 581 z-score: 633.2 E(): 6.2e-28, 40.1% identity in 232 aa overlap and TR:O05781 (EMBL:Z95150) Mycobacterium tuberculosis hypothetical protein (308 aa) (34.9% identity in 301 aa overlap). Contains hydrophobic, possible membrane-spanning region. Contains Pfam match to entry PF00924 UPF0003, Uncharacterized protein family UPF0003 4 3 14 7 [Reporter]SCO0511 (15L22)_PCR/5836 [Gene]SCO0511/5315 NC_003888 SCF6.07, possible dehydrogenase (putative secreted protein), len: 252 aa. Highly similar to many e.g. Pseudomonas paucimobilis SW:LINX_PSEPA (EMBL; D23722) 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (EC 1.1.-.-) (2,5-ddol dehydrogenase) LinX (250 aa), fasta scores opt: 532 z-score: 558.4 E(): 9.7e-24 40.0% identity in 245 aa overlap. Also similar to many Streptomyces coelicolor putative oxidoreductases e.g. TR:O88068 (EMBL; AL031541) putative dehydrogenase SCI35.33C (260 aa), fasta scores opt: 488 z-score: 438.3 E(): 4.1e-19 38.7% identity in 256 aa overlap. Contains a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature and Pfam hits to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 4 3 14 6 [Reporter]SCO1815 (16H22)_PCR/5835 [Gene]SCO1815/5314 NC_003888 SCI28.09c, fabG, probable 3-oxacyl-(acyl-carrier-protein) reductase, len: 234 aa; similar to many e.g. SW:FABG_MYCTU 3-oxacyl-(acyl-carrier-protein) reductase from Mycobacterium tuberculosis (247 aa) fasta scores; opt: 906, z-score: 997.3, E(): 0, (57.3% identity in 234 aa overlap). Contains Pfam match to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature. 4 3 14 5 [Reporter]SCO2961 (17D22)_PCR/5834 [Gene]SCO2961/5313 NC_003888 SCE59.20c, possible integral membrane protein, len: 401 aa; similar to TR:Q9XAP7 (EMBL:AL078618) Streptomyces coelicolor putative integral membrane transferase SC10A7.28c, 363 aa; fasta scores: opt: 397 z-score: 390.6 E(): 2.4e-14; 46.6% identity in 369 aa overlap. Contains possible hydrophobic membrane spanning regions and match to Prosite entry PS00038 Myc-type, 'helix-loop-helix' dimerization domain signature 4 3 14 4 [Reporter]SCO4033 (18P18)_PCR/5833 [Gene]SCO4033/5312 NC_003888 2SC10A7.37, possible membrane protein, len: 96 aa. Contains possible hydrophobic membrane spanning region at C-terminal domain 3 2 20 7 [Reporter]SCO0138 (16C7)_PCR/3448 [Gene]SCO0138/3139 NC_003888 SCJ33.02, possible oxidoreductase, len: 257 aa. Similar to many other putative oxidoreductases including: Streptomyces cinnamonensis SW:DHKR_STRCM (EMBL; Z11511) monensin polyketide synthase putative ketoacyl reductase (EC 1.3.1.-) (261 aa), fasta scores opt: 386 z-score: 426.3 E(): 2.2e-16 35.8% identity in 260 aa overlap. Contains a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature and a Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 4 3 14 3 [Reporter]SCO1875 (19L18)_PCR/5832 [Gene]SCO1875/5311 NC_003888 SCI39.22, possible secreted penicillin-binding protein, len: 484 aa; similar to TR:Q9L656 (EMBL:AF241575) Streptomyces griseus PbpA, 485 aa; fasta scores: opt: 1581 Z-score: 1674.1 bits: 319.2 E(): 1.2e-85; 51.633% identity in 490 aa overlap and to TR:Q9XA17 (EMBL:AL079308) Streptomyces coelicolor putative penicillin binding protein SCH69.17, 490 aa; fasta scores: opt: 1649 Z-score: 1745.9 bits: 332.5 E(): 1.2e-89; 52.965% identity in 489 aa overlap. Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Also contains N-terminal region signal peptide sequence 3 2 20 6 [Reporter]SCO3372 (17O3)_PCR/3447 [Gene]SCO3372/3138 NC_003888 SCE94.23, possible large ATP-binding protein, len: 1010aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). Similar to two closely neighbouring genes SCE94.20 and SCE94.21 suggesting local duplication fasta scores; SCE94.20 opt: 1840, z-score: 1632.1, E(): 0, (48.9% identity in 839 aa overlap), SCE94.21 opt: 984, z-score: 872.5, E(): 0, (43.8% identity in 857 aa overlap). 4 3 14 2 [Reporter]SCO3965 (20D18)_PCR/5831 [Gene]SCO3965/5310 NC_003888 SCBAC25E3.02c, conserved hypothetical protein, len: 178aa: similar to many eg. TR:BAB50242 (EMBL:AP003001) MLL3326 protein from Rhizobium loti (179 aa) fasta scores; opt: 241, Z-score: 269.7, 38.333% identity (41.441% ungapped) in 120 aa overlap. 3 2 20 5 [Reporter]SCO3226 (18K3)_PCR/3446 [Gene]SCO3226/3137 NC_003888 SCE8.19, absA2, two component system response regulator, len: 222aa; sequenced previously therefore identical to TR:Q53894 (EMBL:U51332) AbsA2, predicted response regulator which acts as part of a two component signal transduction system in the global regulation of antibiotic synthesis in Streptomyces coelicolor (222 aa) fasta scores; opt: 1376, z-score: 1584.1, E(): 0, (100.0% identity in 222 aa overlap). Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain, score 138.20, E-value 1.5e-37 and PF00196 GerE, Bacterial regulatory proteins, luxR family, score 70.90, E-value 2.7e-17. Contains a probable helix-turn-helix motif at 171-192aa (Score 1177 (+3.20 SD)). 3 2 20 4 [Reporter]SCP1.15 (19G3)_PCR/3445 [Gene]SCP1.15/3136 NC_003888 SCP1.15, possible membrane protein, len: 74aa; contains possible membrane-spanning hydrophobic regions. 3 2 20 3 [Reporter]SCO1866 (20C3)_PCR/3444 [Gene]SCO1866/3135 NC_003888 SCI39.13, probable condensing enzyme, len: 132 aa; similar to SW:ECTC_MARHA (EMBL:U66614) Marinococcus halophilus L-ectoine synthase EctC, 129 aa; fasta scores: opt: 405 Z-score: 541.3 bits: 105.8 E(): 1.5e-22; 44.800% identity in 125 aa overlap 3 2 20 2 [Reporter]SCO3973 (20K23)_PCR/3443 [Gene]SCO3973/3134 NC_003888 SCBAC25E3.10, possible membrane protein, len: 221aa: no significant database matches. Contains a possible membrane-spanning hydrophobic region. 3 2 19 22 [Reporter]SCO1347 (1K19)_PCR/3441 [Gene]SCO1347/3133 NC_003888 2SCG61.29, possible membrane protein, len: 254 aa; contains possible N-terminal signal sequence and possible membrane-spanning hydrophobic region at C-terminus. 3 2 19 21 [Reporter]SCO0650 (2G19)_PCR/3440 [Gene]SCO0650/3132 NC_003888 SCF91.10, gvpA2, possible gas vesicle synthesis protein, len: 170 aa. Highly similar in the N-terminus to many other gas vesicle synthesis proteins including: Halobacterium salinarium SW:GVA2_HALSA(EMBL:X12862) gas vesicle protein A, chromosomal, GvpA (78 aa), fasta scores opt: 219 z-score: 250.6 E(): 1.4e-06 46.5% identity in 71 aa overlap and Streptomyces coelicolor TR:Q9ZC13 (EMBL:AL033505) putative gas vesicle synthesis protein GvpA (144 aa), fasta scores opt: 367 z-score: 400.3 E(): 6.4e-15 48.2% identity in 141 aa overlap. Contains a Prosite hit to PS00234 Gas vesicles protein GVPa signature 1 and a Pfam match to entry PF00741 Gas_vesicle, Gas vesicle protein. 3 2 19 20 [Reporter]SCO7655 (3C19)_PCR/3439 [Gene]SCO7655/3131 NC_003888 SC10F4.28, possible oxidoreductase, len: 310 aa; similar to many other oxidoreductases, e.g. SW:GS69_BACSU (EMBL:Y14082) Bacillus subtilis general stress protein 69, YhdN, 331 aa; fasta scores: opt: 299 z-score: 349.7 E(): 6.1e-12; 29.9% identity in 261 aa overlap. Contains 2x Pfam matches to entry PF00248 aldo_ket_red, Aldo/keto reductase family 3 2 19 19 [Reporter]SCO7220 (4O15)_PCR/3438 [Gene]SCO7220/3130 NC_003888 SC2H12.19c, unknown, len: 667 aa. Similar to many proteins of undefined function from Streptomyces coelicolor e.g. TR:Q9RJC6(EMBL:AL133171) SCF81.26 (835 aa), opt: 1057 z-score: 1135.5 E():0 38.6% identity in 643 aa overlap. 4 3 13 22 [Reporter]SCO1289 (1D14)_PCR/5829 [Gene]SCO1289/5309 NC_003888 2SCG18.36, probable gntR-family regulatory protein, len: 507 aa; similar to TR:CAB89055 (EMBL:AL353872) Streptomyces coelicolor putative gntR-family regulator SC5G8.04, 462 aa; fasta scores: opt: 1289 z-score: 1152.7 E(): 0; 49.3% identity in 436 aa overlap and to SW:MOCR_RHIME (EMBL:X78503) Rhizobium meliloti probable rhizopine catabolism regulatory protein MocR, 493 aa; fasta scores: opt: 584 z-score: 527.8 E(): 6.5e-22; 34.8% identity in 451 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 4 3 13 21 [Reporter]SCO5868 (2P10)_PCR/5828 [Gene]SCO5868/5308 NC_003888 SC2E9.09, dut, probable deoxyuridine 5'-triphosphate nucleotidohydrolase, len: 183 aa; similar to many eg. DUT_LYCES P32518 deoxyuridine 5'-triphosphate nucleotidohydrolase (169 aa), fasta scores; opt: 299 z-score: 371.4 E(): 1.7e-13, 37.9% identity in 140 aa overlap 4 3 13 20 [Reporter]SCO5906 (3L10)_PCR/5827 [Gene]SCO5906/5307 NC_003888 SC10A5.11, probable transcriptional regulator, len: 198 aa; similar to many members of the tetR family eg. TR:P72569 (EMBL:U58281) TetR homologue WhiR from Streptomyces roseosporus (179 aa), fasta scores; opt: 367 z-score: 569.5 E(): 1.6e-24, 38.3% identity in 175 aa overlap. Contains pr obable helix-turn-helix motif at aa 37 to 58 (Score 1635, +4.76SD) 4 3 13 19 [Reporter]SCO5556 (4H10)_PCR/5826 [Gene]SCO5556/5306 NC_003888 SC1C2.37, histone-like DNA binding protein, len: 218 aa; N-terminus is highly simialar to many bacterial HU proteins e.g. DBHB_ECOLI DNA-binding protein HU-beta (NS1) (HU-1) (90 aa), fasta scores; opt: 230 z-score: 300.3 E(): 1.8e-09, 40.0% identity in 90 aa overlap, and contains PS00045 Bacterial histone-like DNA-binding proteins signature and Pfam match to entry PF00216 Bac_DNA_binding, Bacterial DNA-binding proteins, score 104.40, E-value 9.2e-29. C-terminus contains 18 degenerate repeats of an (A /T)(3-5)KK motif. A similar C-terminal extension is also present in the M. tuberculosis homologue TR:P95109 (EMBL:Z83018) dna-binding protein MTCY349.01 (214 aa), fasta scores; opt: 563 z-score: 543.5 E(): 5e-23, 50.9% identity in 220 aa overlap, and to a lesser extent in the M. leprae homologue. The repeats are simlar to those in hrdB (SC5B8.10, 45.4% identity in 97 aa overlap) 4 3 13 18 [Reporter]SCO6412 (5D10)_PCR/5825 [Gene]SCO6412/5305 NC_003888 SC1A6.01, probable aminotransferase, partial CDS, len: 190 aa; similar to many members of the class-III family of pyridoxal-phosphate-dependent aminotransferases e.g. ARGD_ANASP P54752 anabaena sp. acetylornithine aminotransferase (427 aa), fasta scores; opt: 315 z-score: 402.3 E(): 2.8e-15, 36.0% identity in 189 aa overlap. Contains Pfam matchto entry aminotran_3 PF00202, Aminotransferases class-III pyridoxal-phosphate, score 147.18,SC3C8.31, probable aminotransferase, partial CDS, len: > 270 aa; similar to many members of the class-III pyridoxal-phosphate-dependent aminotransferase family, e.g. ARGD_CORGL acetylornithine aminotransferase (389 aa), fasta scores; opt: 202 z-score: 588.9 E(): 1.5e-25, 36.9% identity in 271 aa overlap. Contains Pfam match to entry aminotran_3 PF00202, Aminotransferases class-III pyridoxal-phosphate, score 171.39. Continues as SC1A6.01 4 3 13 17 [Reporter]SCO5827 (6P6)_PCR/5824 [Gene]SCO5827/5304 NC_003888 SC5B8.17, probable transmembrane transport protein, len: 530 aa; member of the sodium:solute symporter family, similar to many e.g. YJCG_ECOLI P32705 hypothetical protein , (549 aa), fasta scores; opt: 935 z-score: 1534.8 E(): 0, 43.6% identity in 530 aa overlap. Contains Pfam match to en try SSF PF00474, Sodium:solute symporter family, score 338. 37 4 3 13 16 [Reporter]SCO6753 (7L6)_PCR/5823 [Gene]SCO6753/5303 NC_003888 SC6A5.02, probable nucleotide sugar-1-phosphate transferase, len: 250aa; similar to many eg. TR:O05171 (EMBL:Y11985) dNDP-glucose synthase from the avilamycin biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 (355 aa) fasta scores; opt: 192, z-score: 226.4, E(): 2.6e-05. (26.9% identity in 242 aa overlap). Contains Pfam match to entry PF00483 NTP_transferase, Nucleotidyl transferase. 4 3 13 15 [Reporter]SCO4756 (8H6)_PCR/5822 [Gene]SCO4756/5302 NC_003888 SC6G4.34, unknown, len: 118 aa; similar to a hypothe tical protein from Rhizobium meliloti TR:Q52996 (EMBL:L3926 5) (100 aa), fasta scores; opt: 130 z-score: 222.8 E(): 3.8 e-05, 28.8% identity in 73 aa overlap 3 2 19 18 [Reporter]SCO7682 (5K15)_PCR/3437 [Gene]SCO7682/3129 NC_003888 SC4C2.17, probable non-ribosomal peptide synthase, len: 2229 aa; similar to TR:AAG02364 (EMBL:AF210249) Streptomyces verticillus peptide synthetase NRPS2-1 BlmIV, 2626 aa; fasta scores: opt: 2485 z-score: 2429.3 E(): 0; 37.9% identity in 2203 aa overlap, to SW:HMP2_YEREN (EMBL:L18881) Yersinia enterocolitica high-molecular-weight protein 2 Irp2, 2035 aa; fasta scores: opt: 2251 z-score: 2201.3 E(): 0; 33.3% identity in 2022 aa overlap to TR:CAA18919 (EMBL:AL023496) Streptomyces coelicolor probable peptide synthase SC1A6.21, 1407 aa; fasta scores: opt: 1764 z-score: 1430.4 E(): 0; 40.0% identity in 1171 aa overlap and to Streptomyces coelicolor SC4C2.18, 1842 aa; fasta scores: opt: 1742 z-score: 1410.7 E(): 0; 43.0% identity in 997 aa overlap. Contains Pfam matches to entries 3x PF00550 pp-binding, Phosphopantetheine attachment site, 3x PF00668 DUF4, Domain of unknown function DUF4, 2x PF00501 AMP-binding, AMP-binding enzyme and matches to Prosite entries 2x PS00455 Putative AMP-binding domain signature, PS00678 Trp-Asp (WD) repeats signature, 2x PS00012 Phosphopantetheine attachment site and PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 4 3 13 14 [Reporter]SCO7731 (9D6)_PCR/5821 [Gene]SCO7731/5301 NC_003888 SC8D11.22, unknown, len: 183 aa 3 2 19 17 [Reporter]SCO7774 (6G15)_PCR/3436 [Gene]SCO7774/3128 NC_003888 SC5E9.22, possible secreted protein, len: 213aa; region similar to many eg. SW:P96463 (ABFB_STRLI) alpha-L-arabinofuranosidase precursor from Streptomyces lividans (475 aa) fasta scores; opt: 171, z-score: 209.1, E(): 0.00036, 32.2% identity in 177 aa overlap. Contains a possible N-terminal signal sequence. Similar to neighbouring CDS SC5E9.23 fasta scores; opt: 371, z-score: 401.0, E(): 1.1e-16, 42.2% identity in 173 aa overlap. 4 3 13 13 [Reporter]SCO6262 (10P2)_PCR/5820 [Gene]SCO6262/5300 NC_003888 SCAH10.27, possible helicase, len: 977 aa; similar to TR:O53499 (EMBL:AL021924) Mycobacterium tuberculosis helicase, 1013 aa; fasta scores: opt: 1308 z-score: 1370.7 E(): 0; 39.7% identity in 939 aa overlap. Contains two matches to Pfam entries PF00176 SNF2_N, SNF2 and others N-terminal domain and PF00271 helicase_C, Helicases conserved C-terminal domain 3 2 19 16 [Reporter]SCO6168 (7C15)_PCR/3435 [Gene]SCO6168/3127 NC_003888 SC6C5.04c, unknown, len: 262aa 3 2 19 15 [Reporter]SCO5751 (8O11)_PCR/3434 [Gene]SCO5751/3126 NC_003888 SC7C7.06, putative membrane protein, len: 287 aa. Contains possible hydrophobic membrane spanning region 3 2 19 13 [Reporter]SCO6236 (10G11)_PCR/3432 [Gene]SCO6236/3125 NC_003888 SCAH10.01c, possible DNA-binding protein, len: >270 aa; similar to TR:Q9Z543 (EMBL:AL035212) Streptomyces coelicolor putative binding protein, SC9B2.16c, len: 303 aa; fasta scores: opt: 466 z-score: 574.2 E(): 1.3e-24; 34.1% identity in 252 aa overlap. Contains a possible helix-turn-helix motif between residues 612..677 (+3.39 SD),SC2H4.18c, unknown, partial CDS, len: >55 aa; some similarity to the C-terminus of S. coelicolor hypothetical protein SC4G2.03, fasta scores; (E(): 0.018, 32.7% identity in 52 aa overlap) 3 2 19 12 [Reporter]SCO2592 (11C11)_PCR/3431 [Gene]SCO2592/3124 NC_003888 SCC88.03c, possible transferase, len: 942 aa; N-terminal region similar to hypothetical proteins, e. g. TR:O69853 (EMBL:AL023702) Streptomyces coelicolor hypothetical 66.0 kD protein SC1C3.11, 586 aa; fasta scores: opt: 1214 z-score: 1395.6 E(): 0; 40.7% identity in 573 aa overlap and C-terminal region similar to TR:O69854 (EMBL:AL023702) Streptomyces coelicolor putative transferase SC1C3.12, 697 aa; fasta scores: opt: 658 z-score: 754.0 E(): 0; 30.1% identity in 574 aa overlap. Contains Pfam match at N-terminal region to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1 3 2 19 11 [Reporter]SCO4631 (12O7)_PCR/3430 [Gene]SCO4631/3123 NC_003888 SCD82.02c, hypothetical protein, len: 560 aa; similar to C-terminal region of SW:MCRA_ECOLI (EMBL:M76667) Escherichia coli 5-methylcytosine-specific restriction enzyme A (EC 3.1.21.-) McrA, 277 aa; fasta scores: opt: 214 z-score: 250.8 E(): 1.5e-06; 32.8% identity in 131 aa overlap 3 2 19 10 [Reporter]SCO3280 (13K7)_PCR/3429 [Gene]SCO3280/3122 NC_003888 SCE39.30, putative membrane protein, len: 203 aa; similar to another from Streptomyces coelicolor cosmid 5C7 TR:O86731 (EMBL:AL031515) hypothetical protein (314 aa) fasta scores; opt: 163, z-score: 187.7, E(): 0.0038, (31.8% identity in 154 aa overlap). Contains histidine-rich N-terminal region. Contains possible hydrophobic membrane spanning regions 3 2 19 9 [Reporter]SCO1527 (14G7)_PCR/3428 [Gene]SCO1527/3121 NC_003888 SCL2.17c, possible membrane transferase, len: 241 aa; similar to TR:CAB50482 (EMBL:AJ248288) Pyrococcus abyssi CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase PgsA, 186 aa; fasta scores: opt: 286 z-score: 350.3 E(): 4e-12; 33.0% identity in 203 aa overlap and to TR:O06202 (EMBL:Z95387) Mycobacterium tuberculosis hypothetical 23.3 kD protein MTCY1A10.21, 217 aa; fasta scores: opt: 564 z-score: 679.1 E(): 2e-30; 43.4% identity in 212 aa overlap. Contains Pfam match to entry PF01066 CDP-OH_P_transf, CDP-alcohol phosphatidyltransferase and match to Prosite entry PS00379 CDP-alcohol phosphatidyltransferases signature. Contains possible hydrophobic membrane spanning regions 3 2 19 8 [Reporter]SCO1806 (15C7)_PCR/3427 [Gene]SCO1806/3120 NC_003888 SCI33.05c, conserved hypothetical protein, len: 876 aa; similar to TR:O65934 (EMBL:Z95890) Mycobacterium tuberculosis hypothetical 92.2 kDa protein MTCY28.10, 865 aa; fasta scores: opt: 1196 z-score: 907.2 E(): 0; 37.8% identity in 883 aa overlap and to TR:AAK46062 (EMBL:AE007039) Mycobacterium tuberculosis ABC transporter, ATP-binding protein MT1789, 863 aa; fasta scores: opt: 1196 Z-score: 892.8 E(): 4.3e-42; 37.826% identity in 883 aa overlap. Contains 2x Pfam matches to entry PF00498 FHA, FHA domain (N-terminal region) and Pfam match to entry PF00005 ABC_tran, ABC transporter (C-terminal region) and Prosite matches to entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also contains possible hydrophobic membrane spanning regions at C-terminal domain 3 2 19 7 [Reporter]SCO1895 (16O3)_PCR/3426 [Gene]SCO1895/3119 NC_003888 SCI7.13c, probable dihydropicolinate synthase, len: 316 aa; similar to many egs. SW:KDGD_PSEPU 5-dehydro-4-deoxyglutarate dehydratase from Pseudomonas putida (303 aa) fasta scores; opt: 731, z-score: 838.6, E(): 0, (41.1% identity in 302 aa overlap) and SW:DAPA_BACSU dihydropicolinate synthase from Bacillus subtilis (290 aa) fasta scores; opt: 374, z-score: 432.9, E(): 8.8e-17, (29.2% identity in 288 aa overlap). Contains Pfam match to entry PF00701 DHDPS, Dihydrodipicolinate synthetase family. 3 2 19 6 [Reporter]SCO5017 (17K3)_PCR/3425 [Gene]SCO5017/3118 NC_003888 SCK15.19, possible araC family regulatory protein, len: 365 aa; similar to TR:Q54308 (EMBL:X86780) Streptomyces hygroscopicus helix-turn-helix DNA-binding protein, 330 aa; fasta scores: opt: 247 z-score: 294.6 E(): 6.1e-09; 28.6% identity in 346 aa overlap. Contains fam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family and match to Prosite entry PS00041 Bacterial regulatory proteins, araC family signature. Contains also a TTA leucine codon, possible target for bldA regulation 3 2 19 5 [Reporter]SCO1718 (18G3)_PCR/3424 [Gene]SCO1718/3117 NC_003888 SCI11.07c, possible regulator, len: 234 aa; similar to putative transcriptional regulators e.g. TR:O52558 (EMBL:AF040570), RifQ, Amycolatopsis mediterranei putative repressor in rafamycin biosynthetic cluster (242 aa), fasta scores; opt: 325 z-score: 405.5 E(): 3e-15, 33.6% identity in 229 aa overlap. Similar to others from S.coelicolor e.g. SCI30A.23 S.coelicolor possible transcriptional regulator (213 aa) (30.7% identity in 218 aa overlap) 3 2 19 4 [Reporter]SCP1.195c (19C3)_PCR/3423 [Gene]SCP1.195c/3116 NC_003888 SCP1.195c, possible ATP/GTP-binding protein, len: 349aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). Note - correct CDS location may be on opposite strand. 3 2 19 2 [Reporter]SCO5967 (20G23)_PCR/3421 [Gene]SCO5967/3115 NC_003888 SCBAC16H6.02, conserved hypothetical protein, len: 136aa: similar to many eg. TR:Q9EWX7 (EMBL:AL445403) conserved hypothetical protein 2SCI34.02 from Streptomyces coelicolor (146 aa) fasta scores; opt: 257, Z-score: 325.1, 41.667% identity (43.269% ungapped) in 108 aa overlap. 3 2 18 22 [Reporter]SCO1125 (1G19)_PCR/3419 [Gene]SCO1125/3114 NC_003888 2SCG38.18c, unknown, len: 173 aa 3 2 18 21 [Reporter]SCO3182 (2C19)_PCR/3418 [Gene]SCO3182/3113 NC_003888 SCE87.33c, gtaB, UTP-glucose-1-phosphate uridylyltransferase, len: 303 aa. Highly similar to many proteins belonging to the prokaryotic UDPGP family e.g. Bacillus subtilis SW:GTAB_BACSU (EMBL:L12272) UTP-glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) (292 aa), fasta scores opt: 907 z-score: 1054.0 E(): 0 47.1% identity in 293 aa overlap. Contains a Pfam match to entry PF00483 NTP_transferase, Nucleotidyl transferase. 3 2 18 20 [Reporter]SCO6818 (3O15)_PCR/3417 [Gene]SCO6818/3112 NC_003888 SC1A2.27c, possible phosphoglycerate mutase, len: 511 aa. Similar to many 2,3-bisphosphoglycerate-independent phosphoglycerate mutases (EC 5.4.2.1) e.g. Synechocystis sp. (strain PCC 6803) SW:PMGI_SYNY3 (EMBL:D90915) (532 aa), fasta scores opt: 1029 z-score: 1146.1 E():0 36.7% identity in 518 aa overlap and Pseudomonas syringae (pv. tomato) SW:PMGI_PSESM (EMBL:U12776) (510 aa), fasta scores opt: 999 z-score: 1113.0 E(): 0 34.9% identity in 519 aa overlap. Contains a Pfam match to entry PF01676 Metalloenzyme, Metalloenzyme superfamily. 3 2 18 19 [Reporter]SCO7216 (4K15)_PCR/3416 [Gene]SCO7216/3111 NC_003888 SC2H12.15, possible FecCD-family membrane transport protein, len: 368 aa. Highly similar to many including: Yersinia pestis SW:HMUU_YERPE(EMBL:U60647) hemin transport system permease protein HmuU (334 aa), fasta scores opt: 674 z-score: 713.5 E(): 2.9e-32 40.4% identity in 342 aa overlap and Streptomyces coelicolor TR:Q9RKQ5(EMBL:AL133220) FecCD-family membrane transport protein, SCC75A.19 (363 aa), fasta scores opt: 777 z-score: 820.7 E():0 42.6% identity in 371 aa overlap. Contains possible membrane spanning hydrophobic domains, a possible N-terminal signal sequence and a Pfam match to entry PF01032 FecCD_family, FecCD transport family. 3 2 18 18 [Reporter]SCO2393 (5G15)_PCR/3415 [Gene]SCO2393/3110 NC_003888 SC4A7.21, probable aldose 1-epimerase, len: 323 aa; similar to TR:Q9WYB8 (EMBL:AE001710) Thermotoga maritima aldose 1-epimerase TM0282, 356 aa; fasta scores: opt: 677 z-score: 752.5 E(): 0; 47.5% identity in 345 aa overlap, to SW:GALM_ECOLI (EMBL:U13636) Escherichia coli aldose 1-epimerase (EC 5.1.3.3) (mutarotase) GalM, 346 aa; fasta scores: opt: 543 z-score: 605.1 E(): 2.5e-26; 39.0% identity in 346 aa overlap and to S. coelicolor SC4A7.35, 366 aa; fasta scores: opt: 838 z-score: 839.4 E(): 0; 54.2% identity in 347 aa overlap. Contains Pfam match to entry PF01263 Aldose_epim, Aldose 1-epimerase and match to PRosite entry PS00545 Aldose 1-epimerase putative active site 3 2 18 17 [Reporter]SCO7769 (6C15)_PCR/3414 [Gene]SCO7769/3109 NC_003888 SC5E9.17, conserved hypothetical protein, len: 280 aa; similar to many e.g. TR:O31512 (EMBL:Z99107) hypothetical protein from Bacillus subtilis (286 aa) fasta scores; opt: 651, z-score: 727.7, E(): 0, 38.1% identity in 278 aa overlap 3 2 18 16 [Reporter]SCO5658 (7O11)_PCR/3413 [Gene]SCO5658/3108 NC_003888 SC6A9.09c, possible polyamine-binding lipoprotein, len: 399 aa; some similarity to several spermidine /putrescine /putrescine-binding= scores; opt: 389 z-score: 352.1 E(): 2.2e-12, 26.9% identity in 349 aa overlap. Contains N-terminal siganl sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 3 2 18 15 [Reporter]SCO5747 (8K11)_PCR/3412 [Gene]SCO5747/3107 NC_003888 SC7C7.02c, possible regulatory protein, len: 916 aa; N-terminus is similar to S. coloicolor TR:Q53897 (EMBL:X60316) AbaA ORFA (192 aa), fasta scores; opt: 248 z-score: 257.6 E(): 4.1e-07, 34.2% identity in 187 aa overlapC-terminus is similar to several RsbU homologs e.g. Bacillus licheniformis TR:O50229 (EMBL:AF034567) putative sigma B regulator similar to B. subtilis RsbU phosphatase (335 aa), fasta scores; opt: 232 z-score: 196.5 E(): 0.001, 26.3% identity in 240 aa overlap 3 2 18 14 [Reporter]SCO5519 (9G11)_PCR/3411 [Gene]SCO5519/3106 NC_003888 SC8D9.31, unknown, len: 308aa; similar to proteins of undefined function eg. TR:O50444 (EMBL:AL010186) from Mycobacterium tuberculosis (329 aa) fasta scores; opt: 984, z-score: 1121.1, E(): 0, (50.0% identity in 312 aa overlap). 3 2 18 13 [Reporter]SCO3797 (10C11)_PCR/3410 [Gene]SCO3797/3105 NC_003888 SCAC2.05c, possible integral membrane protein, len: 362 aa; similar to TR:O53656 (EMBL:AL021928) Mycobacterium tuberculosis hypothetical 38.0 kDa protein MTV033.13, 367 aa; fasta scores: opt: 586 z-score: 618.4 E(): 6.1e-27; 34.8% identity in 325 aa overlap. Contains Pfam match to entry PF01594 UPF0118, Domain of unknown function DUF20 and possible hydrophobic membrane spanning regions 3 2 18 12 [Reporter]SCO4391 (11O7)_PCR/3409 [Gene]SCO4391/3104 NC_003888 SCD10.23c, conserved hypothetical protein, len: 132 aa; similar to TR:O86547 (EMBL:AL031350) Streptomyces coelicolor hypothetical 15.3 kD protein SC1F2.10, 135 aa; fasta scores: opt: 316 z-score: 419.7 E(): 6.7e-16; 45.1% identity in 122 aa overlap. Contains Pfam match to entry PF01638 DUF24, Protein of unknown function 3 2 18 11 [Reporter]SCO3961 (12K7)_PCR/3408 [Gene]SCO3961/3103 NC_003888 SCD78.28c, serS, Seryl-tRNA synthase, len: 492 aa, similar to many e.g. SYS_ARCFU (EMBL:AE000962)Seryl-tRNA synthase from Archaeoglobus fulgidus (453 aa), fasta scores; opt: 858, z-score: 1275.4, E(): 0, belongs to class II aminoacyl-tRNA synthetase family, contains amino-acyl-transfer RNA synthetases class II signature. Contains Pfam match to entry PF00587 tRNA-synt_2b, tRNA synthetases class II (Gly, His, Pro and Ser), score 205.50, E-value 8e-58. Probable coiled-coil from 57 to 105 (49 residues) Max score: 1.668 (probability 1.00). 3 2 18 10 [Reporter]SCO3085 (13G7)_PCR/3407 [Gene]SCO3085/3102 NC_003888 SCE25.26c, possible membarne protein, len: 117 aa. Contains possible hydrophobic membrane spanning regions 3 2 18 9 [Reporter]SCO3583 (14C7)_PCR/3406 [Gene]SCO3583/3101 NC_003888 SCH66.04, possible integral membrane regulator, len: 254 aa; C-terminal region highly similar to TR:Q54389 (EMBL:M27108) ORFA located alongside the pSAM2 attachment site in Streptomyces lividans (100 aa) fasta scores; opt: 621, z-score: 728.0, E(): 0, (95.0% identity in 100 aa overlap). N-terminal region similar to SW:CARR_MYXXA CarR, light-induced regulator of carotenogenesis in Myxococcus xanthus (221 aa) fasta scores; opt: 120, z-score: 148.1, E(): 0.63, (31.1% identity in 167 aa overlap). Contains possible membrane spanning hydrophobic regions. 3 2 18 8 [Reporter]SCO0743 (15O3)_PCR/3405 [Gene]SCO0743/3100 NC_003888 SCF81.02c, putative secreted protein, len: 94 aa. Contains possible N-terminal region signal peptide sequence 3 3 16 3 [Reporter]SCO6287 (19C22)_PCR/5372 [Gene]SCO6287/4899 NC_003888 SC1G7.13, scoT thioesterase II, len: 268 aa; identical to previously sequenced TR:AAF43096 (EMBL:AF109727) Streptomyces coelicolor A3(2) thioesterase II ScoT, 268 aa. Contains Pfam match to entry PF00975 Thioesterase, Thioesterase domain 3 3 16 2 [Reporter]SCO7468 (20K18)_PCR/5371 [Gene]SCO7468/4898 NC_003888 SCBAC17A6.01c, possible flavin-containing monooxygenase, len: >327aa: similar to many eg. TR:Q9S204 (EMBL:AL109747) putative flavin-containing monooxygenase SCJ21.03 from Streptomyces coelicolor (458 aa) fasta scores; opt: 909, Z-score: 1021.7, 45.399% identity (45.679% ungapped) in 326 aa overlap and SW:P31513 (FMO3_HUMAN) hepatic flavin-containing monooxygenase FMO3 (defects in which cause fish-odour syndrome) (531 aa) fasta scores; opt: 661, Z-score: 743.2, 34.024% identity (35.826% ungapped) in 338 aa overlap. Contains Pfam match to entry PF00743 FMO-like, Flavin-binding monooxygenase-like.,SCBAC14E8.08c, possible monooxigenase (fragment), len: >140 aa; similar to C-terminal region of SW:FMO5_RABIT (EMBL:L08449) Oryctolagus cuniculus dimethylaniline monooxygenase [N-oxide forming] 5 (EC 1.14.13.8) Fmo5, 532 aa; fasta scores: opt: 259 Z-score: 319.3 bits: 66.9 E(): 3.3e-10; 32.353% identity in 136 aa overlap 3 3 15 22 [Reporter]SCO1208 (1K14)_PCR/5369 [Gene]SCO1208/4897 NC_003888 2SCG58.08, hypothetical protein, len: 177 aa; similar to TR:CAB92104 (EMBL:AL356334) Streptomyces coelicolor hypothetical 21.7 kD protein SCD69.03, 200 aa; fasta scores: opt: 195 z-score: 238.2 E(): 8.4e-06; 31.7% identity in 164 aa overlap 3 3 15 21 [Reporter]SCO3137 (2G14)_PCR/5368 [Gene]SCO3137/4896 NC_003888 SCE66.16c, galE1, UDP-glucose 4-epimerase (EC 5.1.3.2), len: 321aa; strongly similar to many eg. SW:P13226 (GALE_STRLI) UDP-glucose 4-epimerase from Streptomyces lividans (329 aa) fasta scores; opt: 1791, z-score: 2020.5, E(): 0, 89.4% identity in 321 aa overlap. Contains Pfam match to entry PF01370 Epimerase, NAD dependent epimerase/dehydratase family. 3 3 15 20 [Reporter]SCO2219 (3C14)_PCR/5367 [Gene]SCO2219/4895 NC_003888 SC10B7.14, hypothetical protein, len: 205 aa; C-terminal region similar to TR:Q9RUW3 (EMBL:AE001974) Deinococcus radiodurans hypothetical protein DR1267, 163 aa; fasta scores: opt: 471 Z-score: 554.2 E(): 3.1e-23; 52.761% identity in 163 aa overlap 3 3 15 19 [Reporter]SCO7013 (4O10)_PCR/5366 [Gene]SCO7013/4894 NC_003888 SC1H10.02C, possible sugar-binding lipoprotein, len: 471 aa. Identical to Streptomyces lividans TR:Q54334(EMBL:U22894) sugar binding lipoprotein precursor, AlgE (471 aa), fasta scores opt: 3154 z-score: 3469.8 E():0 100.0% identity in 471 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 3 3 15 18 [Reporter]SCO2092 (5K10)_PCR/5365 [Gene]SCO2092/4893 NC_003888 SC4A10.25c, conserved hypothetical protein, len: 310 aa; unknown function, similar to many hypothetical proteins e.g. TR:O06212 (EMBL:Z95388) Mycobacterium tuberculosis hypothetical protein (396 aa), fasta scores; opt: 1037 z-score: 1165.6 E(): 0, 53.7% identity in 313 aa overlap, which is longer at the N-terminus. The majority of possible homologues are 300-320 aa in length 3 3 15 17 [Reporter]SCO5792 (6G10)_PCR/5364 [Gene]SCO5792/4892 NC_003888 SC4H2.13, probable transmembrane protein, len: 167 aa; contains three possible transmembrane domains in C-terminus 3 3 15 16 [Reporter]SCO5686 (7C10)_PCR/5363 [Gene]SCO5686/4891 NC_003888 SC5H4.10, probable lipoprotein, len: 561 aa; similar to SW:LPLA_BACSU (EMBL:L03376) Bacillus subtilis LplA, 502 aa; fasta scores: opt: 364 z-score: 402.3 E(): 6e-15; 23.6% identity in 483 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site, possible N-terminal region signal peptide sequence and possible colied-coil region at residues 562..575 3 3 15 15 [Reporter]SCO2470 (8O6)_PCR/5362 [Gene]SCO2470/4890 NC_003888 SC7A8.09c, probable deoxyguanosine triphosphate triphosphohydrolase, len: 424 aa; similar to TR:O52199 (EMBL:AF027507) Mycobacterium smegmatis dDGTPase Dgt, 428 aa; fasta scores: opt: 1272 z-score: 1453.2 E(): 0; 51.9% identity in 430 aa overlap and similar to N-terminus of SW:DGTP_ECOLI (EMBL:M31772) Escherichia coli deoxyguanosine triphosphate triphosphohydrolase Dgt, 504 aa; fasta scores: opt: 128 z-score: 147.9 E(): 0.76; 34.1% identity in 255 aa overlap 3 1 4 14 [Reporter]SCO7182 (8O20)_PCR/1087 [Gene]SCO7182/999 NC_003888 SC8A11.10, probable branched amino acid transport system ATP-binding protein, len: 237 aa; similar to SW:LIVF_ECOLI (EMBL:J05516) Escherichia coli high-affinity branched-chain amino acid transport ATP-binding protein LivF, 237 aa; fasta scores: opt: 687 z-score: 739.1 E(): 0; 50.7% identity in 223 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 1 4 13 [Reporter]SCO6454 (9K20)_PCR/1086 [Gene]SCO6454/998 NC_003888 SC9B5.21, probable ABC transporter ATP-binding protein, len: 335aa; fourth of a five gene operon probably involved in peptide uptake, similar to many eg. SW:DPPD_BACSU dipeptide transport ATP-binding protein, DppD, from Bacillus subtilis (335 aa) fasta scores: opt: 486, z-score: 654.6, E(): 3.6e-29, (37.6% identity in 348 aa overlap). Contains PS00017 ATP /GTP-binding site motif A (P-loop). Also contains Pfam match to entry PF00005 ABC_tran, ABC transporters. 3 1 4 12 [Reporter]SCO2307 (10G20)_PCR/1085 [Gene]SCO2307/997 NC_003888 SCC30.15, possible two-component sensor kinase, len: 409 aa; similar to TR:Q9ZA46 (EMBL:AJ011500) Streptomyces violaceoruber putative two-component sensor kinase GRA-ORF11, 406 aa; fasta scores: opt: 460 z-score: 493.4 E(): 4.9e-20; 33.3% identity in 402 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 4 11 [Reporter]SCO4596 (11C20)_PCR/1084 [Gene]SCO4596/996 NC_003888 SCD20.14c, possible two-component system response regulator, len: 252 aa; similar to TR:CAB92834 (EMBL:AL356892) Streptomyces coelicolor putative two-component response regulator SCC8A.16c, 212 aa; fasta scores: opt: 831 z-score: 957.0 E(): 0; 62.7% identity in 217 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family. Also contains possible helix-turn-helix motif at residues 204..225 (+2.83 SD) 3 1 4 10 [Reporter]SCO2905 (12O16)_PCR/1083 [Gene]SCO2905/995 NC_003888 SCE19A.05c, hypothetical protein, len: 77 aa; unknown function, similar to parts of many sugar permeases e.g. TR:P96159 (EMBL:U65013), MalX, Vibrio furnissii PTS (phosphoenolpyruvate-dependent sugar phosphotransferase system) permease for glucose (523 aa), fasta scores; opt: 217 z-score: 287.9 E(): 1.1e-08, 48.1% identity in 77 aa overlap. Contains Pfam match to entry PF00367 PTS_EIIB, phosphotransferase system, EIIB 3 1 4 9 [Reporter]SCO3857 (13K16)_PCR/1082 [Gene]SCO3857/994 NC_003888 SCH69.27, possible regulatory protein, len: 232aa; similar to SW:NHSR_STRAS proposed regulator of the nosiheptide-resistance genes from Streptomyces actuosus (233 aa) fasta scores; opt: 1231, z-score: 1330.5, E(): 0, (83.2% identity in 226 aa overlap). 3 1 4 8 [Reporter]SCO2991 (14G16)_PCR/1081 [Gene]SCO2991/993 NC_003888 SCE50.19c, unknown, len: 267 aa 3 1 4 7 [Reporter]SCO0910 (15C16)_PCR/1080 [Gene]SCO0910/992 NC_003888 SCM1.43, unknown, len: 433 aa. Similar to a hypothetical protein from Mycobacterium tuberculosis TR:O69672 (EMBL:AL022121) (432 aa), fasta scores opt: 687 z-score: 787.6 E():0 37.5% identity in 421 aa overlap. Also similar to the C-terminus of several Eukaryotic gamma-glutamylcysteine synthetases involved in the de novo synthesis of glutathione in plant chloroplasts, e.g. Brassica juncea (Indian mustard) SW:GSH1_BRAJU(EMBL:Y10848) glutamate-cysteine ligase precursor (EC 6.3.2.2) (514 aa), fasta scores opt: 428 z-score: 490.5 E(): 6.1e-20 30.4% identity in 362 aa overlap 3 1 4 6 [Reporter]SCO2998 (16O12)_PCR/1079 [Gene]SCO2998/991 NC_003888 SCE99.05, possible transferase, len: 573 aa; N-terminal domain similar to TR:AAG01876 (EMBL:AF292031) Mesorhizobium huakuii glucosyltransferase Exo1, 340 aa; fasta scores: opt: 312 z-score: 366.8 E(): 6.8e-13; 30.3% identity in 337 aa overlap. Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases 3 1 4 5 [Reporter]SCO3564 (17K12)_PCR/1078 [Gene]SCO3564/990 NC_003888 SCH5.27, possible sodium/proton antiporter, len: 474aa; similar to many eg. TR:Q56725 (EMBL:D28504) sodium/proton antiporter from Vibrio parahaemolyticus (383 aa) fasta scores; opt: 931, z-score: 1045.1, E(): 0, (40.1% identity in 377 aa overlap). Contains probable membrane spanning hydrophobic regions. 3 3 15 14 [Reporter]SCO7835 (9K6)_PCR/5361 [Gene]SCO7835/4889 NC_003888 SC8E7.32, unknown, len: 136 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1Y2(EMBL:AL109973) hypothetical 15.5 kd protein, SCJ30.07c (136 aa). 3 3 15 13 [Reporter]SCO5707 (10G6)_PCR/5360 [Gene]SCO5707/4888 NC_003888 SC9F2.09c, small conserved hypothetical protein, len: 97aa; similar to eg. TR:O33252 (EMBL:Z97559) hypothetical protein from Mycobacterium tuberculosis (97 aa) fasta scores; opt: 397, z-score: 552.5, E(): 1.8e-23, (64.9% identity in 97 aa overlap). 3 3 15 12 [Reporter]SCO2285 (11C6)_PCR/5359 [Gene]SCO2285/4887 NC_003888 SCC75A.31, possible oxidoreductase, len: 351 aa. Similar to many Eukaryotic dimeric dihydrodiol dehydrogenases e.g. Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey) TR:BAA83489(EMBL:AB021932) dimeric dihydrodiol dehydrogenase (EC 1.3.1.20) (334 aa), fasta scores opt: 741 z-score: 868.2 E():0 39.1% identity in 335 aa overlap. Also similar to Prokaryotic oxidoreductases e.g. Streptomyces antibioticus TR:AAD55450(EMBL:AF055579) putative 3-ketoreductase (328 aa), fasta scores opt: 434 z-score: 510.8 E(): 4.5e-21 34.0% identity in 300 aa overlap. Contains a Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family. 3 3 15 11 [Reporter]SCO4122 (12O2)_PCR/5358 [Gene]SCO4122/4886 NC_003888 SCD72A.08, possible MarR-family transcriptional regulator, len: 182 aa; similar to many e.g. SW:P42195 (PECS_ERWCH) regulatory protein PecS from Erwinia chrysanthemi (166 aa) fasta scores; opt: 183, z-score: 231.8, E(): 1.9e-05, 24.3% identity in 152 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family and Prosite match to PS00519 Bacterial regulatory proteins, asnC family signature 3 3 15 10 [Reporter]SCO3032 (13K2)_PCR/5357 [Gene]SCO3032/4885 NC_003888 SCE34.13c, possible secreted protein, len: 506 aa. Contains possible N-terminal region signal peptide sequence 3 3 15 9 [Reporter]SCO3580 (14G2)_PCR/5356 [Gene]SCO3580/4884 NC_003888 SCH66.01, partial CDS, possible transpeptidase, len: >366 aa; similar to the C-terminal region of many penicillin-binding transpeptidases eg. SW:PBPD_BACSU penicillin-binding protein 4 from Bacillus subtilis (624 aa) fasta scores; opt: 298, z-score: 300.3, E(): 2.1e-09, (32.0% identity in 228 aa overlap). Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain, score 3.70, E-value 1.6e-08.,SCH17.14, probable penicillin-binding protein, partial CDS, len: >427 aa; similar to many e.g. TR:P72351 (EMBL:S82044), Pon1, Mycobacterium leprae penicillin-binding protein (821 aa), fasta scores; opt: 735 z-score: 808.8 E(): 0, 36.8% identity in 413 aa overlap. Similar to others from S. coelicolor e.g. SCH24.23 (EMBL:AL049826) probable penicillin-binding protein (795 aa) (28.8% identity in 420 aa overlap). Contains hydrophobic, possible membrane-spanning region in the N-terminus. Contains Pfam match to entry PF00912 Transglycosyl, Transglycosylase 3 3 15 8 [Reporter]SCO0344 (15C2)_PCR/5355 [Gene]SCO0344/4883 NC_003888 SCF41.03, possible GntR family DNA-binding regulator, len: 414 aa. C-terminus similar to Streptomyces coelicolor TR:Q9ZBS9 (EMBL; AL034446) putative GntR family transcriptional regulator SC1A9.23 (273 aa), fasta scores opt: 332 z-score: 363.0 E(): 7.2e-13 31.9% identity in 254 aa overlap. The N-terminus is weakly similar to other DNA-binding proteins e.g. Clavibacter michiganensis subsp. sepedonicus TR:O86023 (EMBL; AF079817) putative transposase (320 aa), fasta scores opt: 158 z-score:0.0 E(): 0.0 30.04% identity in 233 aa overlap. Contains a Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. Contains two potential helix- turn-helix motifs: between aa 135..156 score (+5.36 SD) and residues 69..90 score (+4.14 SD). 3 3 15 7 [Reporter]SCO0376 (15O22)_PCR/5354 [Gene]SCO0376/4882 NC_003888 SCF62.02, possible transcriptional regulator, len: 200 aa. Similar to several proteins of undefined function from Streptomyces sp. e.g. Streptomyces coelicolor TR:CAB51134 (EMBL; Y18817) hypothetical 20.6 KD protein (190 aa), fasta scores opt: 352 z-score: 424.9 E(): 2.6e-16 37.0% identity in 189 aa overlap. Contains a Pfam match to entry PF01381 HTH_3, Helix-turn-helix between residues 29..50 (+5.17 SD). 3 3 15 6 [Reporter]SCO0791 (16K22)_PCR/5353 [Gene]SCO0791/4881 NC_003888 SCF43.02, possible integral membrane protein, len: 379 aa. Almost identical to Streptomyces lividans TR:P72466(EMBL:Z70724) substrate hyphae associated protein, Orf3 (329 aa), fasta scores opt: 2115 z-score: 2334.5 E():0 99.4% identity in 329 aa overlap. Also weakly similar to Mycobacterium tuberculosis SW:Y0BU_MYCTU(EMBL:Z77724) hypothetical 36.8 KD protein (355 aa), fasta scores opt: 208 z-score: 236.0 E(): 9.3e-06 26.1% identity in 303 aa overlap and to a downstream CDS, SCF43.07, putative integral membrane protein (419 aa), fasta scores opt: 276 z-score: 252.1 E(): 1.2e-08 28.1% identity in 366 aa overlap. Note SCF43.02 is 50 aa longer than Streptomyces lividans Orf3. Contains possible membrane spanning hydrophobic domains. 3 3 15 5 [Reporter]SCO6585 (17G22)_PCR/5352 [Gene]SCO6585/4880 NC_003888 SC8A6.06, sucC1, probable succinyl-coa synthetase beta chain, len: 383 aa; highly similar to many e.g. SUCC_MYCT U succinyl-coa synthetase beta chain (EC 6.2.1.5) (387 aa), fasta scores; opt: 901 z-score: 1682.1 E(): 0, 56.1% identity in 387 aa overlap. Contains Pfam match to entry PF00549 ligase-CoA, CoA-ligases, score 119.60, E-value 2.9e-32 3 1 4 4 [Reporter]SCO4089 (18G12)_PCR/1077 [Gene]SCO4089/989 NC_003888 SCD25.25c, valine dehydrogenase (EC 1.4.1.-), len: 364 aa. Previously sequenced and characterised Streptomyces coelicolor SW:VDH_STRCO (EMBL; L13455) valine dehydrogenase (EC 1.4.1.-) (VALDH). Contains Pfam match to entry PF00208 GLFV_dehydrog, Glutamate/Leucine/Phenylalanine/Valine dehydrogenase and a Prosite hit to PS00074 Glu / Leu / Phe 3 1 4 3 [Reporter]SCO6295 (19C12)_PCR/1076 [Gene]SCO6295/988 NC_003888 SCBAC8D1.08, possible ABC transporter ATP-binding protein, len: 662 aa; similar to TR:Q9KKV4 (EMBL:AE004426) Vibrio cholerae ABC transporter ATP-binding protein VCA0996, 619 aa; fasta scores: opt: 1081 Z-score: 1044.0 bits: 203.4 E(): 1.4e-50; 35.859% identity in 594 aa overlap. Contains Pfam matches to entries PF00664 ABC_membrane, ABC transporter transmembrane region and PF00005 ABC_tran, ABC transporter and matches to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature and PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. Also contains 2x degenerate repeat: VDGDGRDDGDGR(D/R)D and possible hydrophobic membrane spanning regions at N-terminal domain 3 1 4 2 [Reporter]SCO0420 (20K8)_PCR/1075 [Gene]SCO0420/987 NC_003888 SCF51.19c, possible homoserine dehydrogenase, len: 344 aa; similar to SW:DHOM_SYNY3 (EMBL:D64001) Synechocystis sp. (strain PCC 6803) homoserine dehydrogenase (EC 1.1.1.3) (HDH), 433 aa; fasta scores: opt: 284 z-score: 307.6 E(): 9.2e-10; 29.0% identity in 335 aa overlap and to TR:O26517 (EMBL:AE000826) Methanobacterium thermoautotrophicum homoserine dehydrogenase homologue MTH417, 340 aa; fasta scores: opt: 332 z-score: 359.9 E(): 1.1e-12; 30.1% identity in 335 aa overlap. Contains Prosite match to entry PS01042 Homoserine dehydrogenase signature 3 1 3 22 [Reporter]SCO4197 (1K4)_PCR/1073 [Gene]SCO4197/986 NC_003888 2SCD46.11, possible MarR-family regulator, len: 155 aa; similar to many e.g. TR:Q9RK19 (EMBL:AL109974) putative MarR-family transcriptional regulator from Streptomyces coelicolor (168 aa) fasta scores; opt: 192, z-score: 240.7, E(): 6.4e-06, 34.2% identity in 149 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family. 3 1 3 21 [Reporter]SCO0776 (2G4)_PCR/1072 [Gene]SCO0776/985 NC_003888 3SCF60.08c, possible integral membrane protein, len: 338 aa; identical to TR:O85699 (EMBL:AF072709) Streptomyces lividans hypothetical 35.5 kDa protein, 338 aa. Contains Pfam match to entry PF01545 Cation_efflux, Cation efflux family. Also contains possible hydrophobic membrane spanning regions 3 1 3 20 [Reporter]SCO7336 (3C4)_PCR/1071 [Gene]SCO7336/984 NC_003888 SC4G10.15c, pepA, unknown, len: 90 aa. Previously sequenced region: Streptomyces coelicolor TR:O54206(EMBL:AJ001206) hypothetical protein, PepA (90 aa). 3 1 3 19 [Reporter]SCO6152 (3O24)_PCR/1070 [Gene]SCO6152/983 NC_003888 SC1A9.16c, small hydrophobic protein (putative membrane protein), len: 160 aa. Contains possible hydrophobic membrane spanning regions 3 1 3 18 [Reporter]SCO7086 (4K24)_PCR/1069 [Gene]SCO7086/982 NC_003888 SC3A4.12c, possible MerR-family transcriptional regulator, len: 115 aa. Similar to many regulators including: Thiobacillus ferrooxidans SW:MERR_THIFE (EMBL:M57717) mercuric resistance operon regulatory protein (fragment) (122 aa), fasta scores opt: 166 z-score: 229.9 E(): 2.4e-05 33.0% identity in 106 aa overlap. Conatins a Prosite hit to PS00552 Bacterial regulatory proteins, merR family signature and a Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family with a putative helix-turn-helix motif situated between residues 4..25 (+4.59 SD). 3 1 3 17 [Reporter]SCO6562 (5G24)_PCR/1068 [Gene]SCO6562/981 NC_003888 SC4B5.12c, probable oxidoreductase, len: 323; similar to several hypothetical /putative= probable 2-dehydropantoate 2-reductase from Archaeoglobus fulgidus (294 aa), fasta scores; opt: 245, z-score: 404.0, E(): 3.3e-15, (29.1% identity in 313 aa overlap). 3 1 3 16 [Reporter]SCO6732 (6C24)_PCR/1067 [Gene]SCO6732/980 NC_003888 SC5F2A.15, possible fatty acid oxidative multifunctional enzyme, len: 726aa; similar to SW:FAOB_PSEFR fatty oxidation complex alpha subunit from Pseudomonas fragi (715 aa) fasta scores; opt: 1174, z-score: 1211.3, E(): 0, (33.2% identity in 717 aa overlap). Also similar to TR:O53872 (EMBL:AL022004) hypothetical protein from Mycobacterium tuberculosis (720 aa) fasta scores; opt: 3034, z-score: 3128.4, E(): 0, (64.3% identity in 717 aa overlap). Also similar to SC6A5.38 (EMBL:AL049485) possible fatty oxidation protein from Streptomyces coelicolor (733 aa) fasta scores; opt: 4069, z-score: 4004.0, E(): 0, (84.6% identity in 729 aa overlap). Contains Pfam matches to entries PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase and PF00378 ECH, Enoyl-CoA hydratase/isomerase family and Prosite match to PS00923 Aspartate and glutamate racemases signature 1. 3 3 15 4 [Reporter]SCO0958 (18C22)_PCR/5351 [Gene]SCO0958/4879 NC_003888 SCM11.13c, hypothetical protein, len: 446 aa; similar to various Mycobacterium tuberculosis hypothetical proteins, e.g. TR:O07035 (EMBL:Z95150) Mycobacterium tuberculosis hypothetical 50.7 kD protein RV3130c, 463 aa; fasta scores: opt: 491 z-score: 529.2 E(): 4.3e-22; 30.2% identity in 464 aa overlap 3 3 15 3 [Reporter]SCP1.122 (19O18)_PCR/5350 [Gene]SCP1.122/4878 NC_003888 SCP1.122, unknown, len: 246aa; regions similar to other proteins of undefined function eg. SW:P71690 (YE21_MYCTU) hypothetical protein from Mycobacterium tuberculosis (301 aa) fasta scores; opt: 194, z-score: 231.0, E(): 2.1e-05, 36.4% identity in 132 aa overlap. 3 3 15 2 [Reporter]SCO2812 (20G18)_PCR/5349 [Gene]SCO2812/4877 NC_003888 SCBAC17F8.03, possible drug efflux protein, len: 416aa: similar to many eg. TR:Q9LA76 (EMBL:AF148322) valanimycin-resistance protein from Streptomyces viridifaciens (402 aa) fasta scores; opt: 582, Z-score: 614.9, 32.405% identity (33.952% ungapped) in 395 aa overlap and TR:CAC41338 (EMBL:AJ307714) tertracycline resistance protein from Escherichia coli (398 aa) fasta scores; opt: 262, Z-score: 281.3, 28.056% identity (32.268% ungapped) in 360 aa overlap. Contains possible membrane-spanning hydrophobic regions. 3 3 14 22 [Reporter]SCO0991 (1G14)_PCR/5347 [Gene]SCO0991/4876 NC_003888 2SCG2.04, conserved hypothetical protein, len: 287 aa; similar to TR:Q9XDM4 (EMBL:AF026270) Salmonella enterica serovar Typhimurium hypothetical protein PduX, 300 aa; fasta scores: opt: 217 z-score: 267.6 E(): 2.2e-07; 27.1% identity in 266 aa overlap 3 3 14 21 [Reporter]SCO6589 (2C14)_PCR/5346 [Gene]SCO6589/4875 NC_003888 SC8A6.10, fusB, possible alternative elongation fact or G, len: 686 aa; highly similar to many e.g. EFG_BACSU el ongation factor g (ef-g) (691 aa), fasta scores; opt: 2058 z-score: 2027.9 E(): 0, 47.5% identity in 692 aa overlap. A lso 46% identical to S. coelicolor fusA EFG_STRCO (fragment ) (93 aa). Contains PS00017 ATP/GTP-binding site motif A (P -loop), PS00301 GTP-binding elongation factors signature an d Pfam matches to entry PF00009 GTP_EFTU, Elongation factor Tu family, score 521.10, E-value 7.8e-153 and Pfam match t o entry PF00679 EFG_C, Elongation factor G C-terminus, scor e 214.30, E-value 1.8e-60 3 3 14 20 [Reporter]SCO5910 (3O10)_PCR/5345 [Gene]SCO5910/4874 NC_003888 SC10A5.15, putative membrane protein, len: 114 aa. Contains possible hydrophobic membrane spanning regions 3 3 14 19 [Reporter]SCO6946 (4K10)_PCR/5344 [Gene]SCO6946/4873 NC_003888 SC1G8.18, unknown, len: 79 aa. 3 3 14 18 [Reporter]SCO5447 (5G10)_PCR/5343 [Gene]SCO5447/4872 NC_003888 SC3D11.04c, probable neutral zinc metalloprotease, len: 684aa; similar to many eg. SW:P43263 (NPRE_BACBR) bacillolysin precursor from Bacillus brevis (527 aa) fasta scores; opt: 648, z-score: 662.4, E(): 1.7e-29, 34.6% identity in 480 aa overlap. Also similar to the neighbouring CDS SC3D11.03c fasta scores; opt: 2442, z-score: 2055.1, E(): 0, 69.9% identity in 544 aa overlap and TR:CAB69762 (EMBL:AL137187) putative metalloproteinase from Streptomyces coelicolor fasta scores; opt: 1265, z-score: 1067.4, E(): 0, 50.2% identity in 560 aa overlap. Contains Pfam match to entry PF01447 Peptidase_M4, Thermolysin family peptidase and Prosite match to PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Contains possible hydrophobic membrane spanning region 2 2 21 3 [Reporter]SCO7514 (20F3)_PCR/2962 [Gene]SCO7514/2699 NC_003888 SCBAC25F8.06, possible integral membrane protein, len: 166 aa; similar to TR:Q9RIW7 (EMBL:AL121854) Streptomyces coelicolor putative transmembrane transport protein SCJ33.11, 163 aa; fasta scores: opt: 262 Z-score: 315.0 bits: 64.6 E(): 6.2e-10; 35.393% identity in 178 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 14 17 [Reporter]SCO5787 (6C10)_PCR/5342 [Gene]SCO5787/4871 NC_003888 SC4H2.08, unknown, len: 505 aa; highly similar to hypothetical proteins from many organisms e.g. YLEA_ECOLI P77645 hypothetical 53.7 kd protein in cute- (474 aa), fatsa scores; opt: 1101 z-score: 1288.4 E(): 0, 40.9% identity in445 aa overlap. Contains PS01278 Uncharacterized protein family UPF0004 signature 2 2 21 2 [Reporter]SCO5097 (20N23)_PCR/2961 [Gene]SCO5097/2698 NC_003888 SCBAC28G1.23, short-chain oxidoreductase, len: 276 aa; identical to previously sequenced TR:Q9X9U8 (EMBL:Y18817) Streptomyces coelicolor A3(2) oxidoreductase of short-chain, 276 aa. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 3 3 14 16 [Reporter]SCO6756 (7O6)_PCR/5341 [Gene]SCO6756/4870 NC_003888 SC6A5.05, possible glycosyltransferase, len: 290aa; similar to many eg. TR:E1388141 (EMBL:AL035478) putative transferase from Streptomyces coelicolor (344 aa) fasta scores; opt: 202, z-score: 235.1, E(): 8.6e-06, (28.1% identity in 317 aa overlap). Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases. Contains possible membrane spanning hydrophobic regions. 3 1 3 15 [Reporter]SCO6950 (7O20)_PCR/1066 [Gene]SCO6950/979 NC_003888 SC6F7.03, unknown, len: 467 aa. Weakly similar to several including: Streptomyces coelicolor TR:Q9X7U7(EMBL:AL049863) putative regulatory protein, SC5H1.40C (502 aa), fasta scores opt: 315 z-score: 356.4 E(): 2.1e-12 26.4% identity in 488 aa overlap. 2 2 20 22 [Reporter]SCO1350 (1N19)_PCR/2959 [Gene]SCO1350/2697 NC_003888 2SCG61.32, unknown, len: 206 aa; weakly similar to others from Streptomyces coelicolor eg. TR:Q9X7Q5 (EMBL:AL049587) hypothetical protein (161 aa) fasta scores; opt: 132, z-score: 160.8, E(): 0.18, 32.6% identity in 135 aa overlap. 3 1 3 14 [Reporter]SCO7178 (8K20)_PCR/1065 [Gene]SCO7178/978 NC_003888 SC8A11.06, probable transcriptional regulatory protein, len: 122 aa; similar to SW:BRPA_STRHY (EMBL:M64783) Streptomyces hygroscopicus bialaphos biosynthetic pathway regulatory protein BrpA, 256 aa; fasta scores: opt: 165 z-score: 209.2 E(): 0.00035; 34.4% identity in 122 aa overlap. Contains possible helix-turn-helix motif at residues 77..98 (+4.46 SD) 2 2 20 21 [Reporter]SCO3180 (2J19)_PCR/2958 [Gene]SCO3180/2696 NC_003888 SCE87.31c, moaC, probable molybdenum cofactor biosynthesis protein, len: 170 aa. Highly similar to many including: Mycobacterium tuberculosis TR:O53876 (EMBL:AL022004) MoaC protein (167 aa), fasta scores opt: 627 z-score: 742.2 E():0 61.0% identity in 164 aa overlap and Escherichia coli SW:MOAC_ECOLI(EMBL:X70420) molybdenum cofactor biosynthesis protein C (160 aa), fasta scores opt: 459 z-score: 547.5 E(): 4e-23 54.2% identity in 153 aa overlap. Contains a possible membrane spanning hydrophobic domain. 3 1 3 13 [Reporter]SCO7165 (9G20)_PCR/1064 [Gene]SCO7165/977 NC_003888 SC9A4.27c, probable sugar-binding integral membrane transport protein, len: 319 aa; similar to TR:Q9RJV6 (EMBL:AL117387) Streptomyces coelicolor putative binding-protein-dependent transport protein SCF41.13, 280 aa; fasta scores: opt: 1039 z-score: 1201.7 E(): 0; 57.2% identity in 264 aa overlap and to SW:MSMG_STRMU (EMBL:M77351) Streptococcus mutans multiple sugar-binding transport system permease protein MsmG, 277 aa; fasta scores: opt: 512 z-score: 596.7 E(): 9.1e-26; 32.4% identity in 275 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 2 2 20 20 [Reporter]SCO7659 (3F19)_PCR/2957 [Gene]SCO7659/2695 NC_003888 SC10F4.32c, probable oxidoreductase, len: 373 aa; similar to SW:TETX_BACFR (EMBL:M37699) Bacteroides fragilis tetracycline resistance protein TetX, 388 aa; fasta scores: opt: 524 z-score: 576.3 E(): 1.4e-24; 30.2% identity in 374 aa overlap and to TR:CAB94636 (EMBL:AL359215) Streptomyces coelicolor hypothetical protein SC2H12.22, 373 aa; fasta scores: opt: 1709 z-score: 1482.3 E(): 0; 71.2% identity in 375 aa overlap. Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase 3 1 3 12 [Reporter]SCO2303 (10C20)_PCR/1063 [Gene]SCO2303/976 NC_003888 SCC30.11, unknown, len: 55 aa 2 2 20 19 [Reporter]SCO5612 (4B19)_PCR/2956 [Gene]SCO5612/2694 NC_003888 SC2E1.29, unknown prophage ATP binding protein, len: 756 aa; some similarity to TR:Q38030 (EMBL:X76288) Streptomyces Bacteriophage phi-C31 early region ORF9 (519 aa), fasta scores; opt: 360 z-score: 374.4 E(): 1.3e-13, 24.4% identity in 553 aa overlap, and to a putative prophage ORF from M. tuberculosis TR:O06608 (EMBL:Z95586) MTCY336.22 (471 aa), fasta scores; opt: 655 z-score: 535.5 E(): 1.4e-22, 29.8% identity in 416 aa overlap. Contains 2x PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 3 11 [Reporter]SCO4519 (11O16)_PCR/1062 [Gene]SCO4519/975 NC_003888 SCD35.26, possible integral membrane protein, len: 198 aa; similar to TR:Q9KYA1 (EMBL:AL356813) putative integral membrane protein SCK15.03 from Streptomyces coelicolor (213 aa) fasta scores; opt: 349, Z-score: 400.7, E(): 1.1e-14, 37.363% identity (37.989% ungapped) in 182 aa overlap. Contains possible hydrophobic membrane spanning regions 2 2 20 18 [Reporter]SCO7685 (5N15)_PCR/2955 [Gene]SCO7685/2693 NC_003888 SC4C2.20, conserved hypothetical protein, len: 677 aa; similar to TR:Q9Z602 (EMBL:AF116557) Pseudomonas aeruginosa protein necessary for the formation of the siderophore pyochelin PchG, 349 aa; fasta scores: opt: 559 z-score: 589.4 E(): 2.7e-25; 36.6% identity in 350 aa overlap and to Streptomyces coelicolor hypothetical protein SC4C2.23, 242 aa; fasta scores: opt: 705 z-score: 579.4 E(): 1.3e-26; 50.2% identity in 243 aa overlap 3 1 3 10 [Reporter]SCO2923 (12K16)_PCR/1061 [Gene]SCO2923/974 NC_003888 SCE19.23c, putative membrane protein, len: 224 aa; unknown function, probable CDS suggested by probable CDS suggested by GC frameplot, positional base preference and amino acid composition. The N-terminus is Pro-rich. Weak similarity to adjacent CDS SCE19.22c, hypothetical protein (148 aa) (29.1% identity in 158 aa overlap). Contains possible hydrophobic membrane spanning regions 2 2 20 17 [Reporter]SCO2191 (6J15)_PCR/2954 [Gene]SCO2191/2692 NC_003888 SC5F7.10, probable oxidoreductase, len: 467 aa; low similarity to many e.g. SW:PUO_MICRU putrescine oxidase from Micrococcus rubens (478 aa) fasta scores; opt: 195, z-score: 192.3, E(): 0.0022, (42.7% identity in 110 aa overlap). 3 1 3 9 [Reporter]SCO3895 (13G16)_PCR/1060 [Gene]SCO3895/973 NC_003888 SCH24.17c, putative membrane protein, len: 787 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains possible hydrophobic membrane spanning regions 2 2 20 16 [Reporter]SCO6171 (7F15)_PCR/2953 [Gene]SCO6171/2691 NC_003888 SC6C5.07, probable iron-sulphur binding oxidoreductase subunit, len: 196aa; similar to many eg. TR:P95635 (EMBL:U65440) 4-hydroxybenzoyl-CoA reductase from Rhodopseudomonas palustris (163 aa) fasta scores; opt: 288, z-score: 720.8, E(): 7.4e-33, (42.2% identity in 187 aa overlap) and TR:P72223 (EMBL:X98131) quinoline 2-oxidoreductase from Pseudomonas putida (168 aa) fasta scores; opt: 264, z-score: 703.7, E(): 6.6e-32, (40.4% identity in 198 aa overlap). Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. 3 1 3 8 [Reporter]SCO3812 (14C16)_PCR/1059 [Gene]SCO3812/972 NC_003888 SCGD3.13, probable gntR-family transcriptional regulator, len: 115 aa; weakly similar to several transcriptional regulators e.g. to the N-terminal half of TR:O70026 (EMBL:X80774), traR, transcriptional repressor of TraB from Streptomyces ghanaensis plasmid pSG5 (246 aa), fasta scores; opt: 173 z-score: 224.1 E(): 3.7e-05, 35.6% identity in 87 aa overlap. Similar to TR:O34712 (EMBL:AF008220) Bacillus subtilis putative transcription regulator (130 aa) (28.2% identity in 117 aa overlap). Weakly similar to many from S.coelicolor e.g. (EMBL:AL049573), SCE39.19, possible gntR-family regulator (251 aa) (37.1% identity in 97 aa overlap). Contains probable helix-turn-helix motif at aa 38-59 (Score 1394, +3.93 SD). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 2 2 20 15 [Reporter]SCO5228 (8B15)_PCR/2952 [Gene]SCO5228/2690 NC_003888 SC7E4.25c, possible acetyltransferase, len: 168 aa; similar to TR:Q9S2P7 (EMBL:AL096872) Streptomyces coelicolor putative acetyltransferase SC5F7.12c, 169 aa; fasta scores: opt: 151 z-score: 187.3 E(): 0.0058; 36.2% identity in 141 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 3 1 3 7 [Reporter]SCO0102 (15O12)_PCR/1058 [Gene]SCO0102/971 NC_003888 SCJ11.31, unknown, len: 148 aa 3 1 3 6 [Reporter]SCO0853 (16K12)_PCR/1057 [Gene]SCO0853/970 NC_003888 SCM2.06, possible membrane protein, len: 132 aa. Contains a possible membrane spanning hydrophobic domain. 3 3 14 15 [Reporter]SCO2464 (8K6)_PCR/5340 [Gene]SCO2464/4869 NC_003888 SC7A8.03, probable ABC transporter, len: 642 aa; similar to TR:Q9WYC4 (EMBL:AE001710) Thermotoga maritima ABC transporter, ATP-binding protein, 598 aa; fasta scores: opt: 1520 z-score: 1587.7 E(): 0; 40.3% identity in 613 aa overlap and to SW:YC72_MYCTU (EMBL:Z77137) Mycobacterium tuberculosis hypothetical ABC transporter ATP-binding protein RV1273c, 631 aa; fasta scores: opt: 2325 z-score: 2426.2 E(): 0; 57.6% identity in 632 aa overlap. Contains two Pfam matches to entry PF00664 ABC_membrane, ABC transporter transmembrane region and two matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 3 14 14 [Reporter]SCO7734 (9G6)_PCR/5339 [Gene]SCO7734/4868 NC_003888 SC8D11.25, unknown, len: 185 aa 3 3 14 13 [Reporter]SCO5711 (10C6)_PCR/5338 [Gene]SCO5711/4867 NC_003888 SC9F2.05c, probable riboflavin kinase (FAD synthetase), len: 318 aa; similar to many e.g. SW:RIBF_CORAM riboflavin kinase from Corynebacterium ammoniagenes (338 aa) fasta scores; opt: 801, z-score: 904.5, E(): 0, (45.4% identity in 326 aa overlap) 3 3 14 12 [Reporter]SCO2359 (11O2)_PCR/5337 [Gene]SCO2359/4866 NC_003888 SCC8A.17c, possible two-component sensor kinase, len: 372aa; similar to many from Streptomyces coelicolor eg. TR:CAB81858 (EMBL:AL161691) putative two-component system sensor kinase (431 aa) fasta scores; opt: 690, z-score: 750.8, E(): 0, 39.3% identity in 399 aa overlap and SW:P27668 (UHPB_SALTY) sensor protein UhpB from Salmonella typhimurium (500 aa) fasta scores; opt: 269, z-score: 295.6, E(): 5.3e-09, 25.5% identity in 377 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 14 11 [Reporter]SCO4118 (12K2)_PCR/5336 [Gene]SCO4118/4865 NC_003888 SCD72A.04c, possible tetR-family transcriptional regulator, len: 297 aa; similar to many proposed transcriptional regulators e.g. TR:CAB58280 (EMBL:AL121849) putative tetR-family transcriptional regulator from Streptomyces coelicolor (194 aa) fasta scores; opt: 238, z-score: 239.0, E(): 7.5e-06, 32.3% identity in 189 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a helix-turn-helix motif 63-84 aa 3 3 14 10 [Reporter]SCO3185 (13G2)_PCR/5335 [Gene]SCO3185/4864 NC_003888 SCE22.02, possible Na+/H+ antiporter, len: 515 aa; similar to TR:Q9XAJ9 (EMBL:AL079348) Streptomyces coelicolor putative Na(+)/H(+) antiporter SC66T3.14c, 514 aa; fasta scores: opt: 1828 z-score: 1992.7 E(): 0; 55.1% identity in 497 aa overlap. Contains Pfam match to entry PF00999 Na_H_Exchanger, Sodium/hydrogen exchanger family and possible hydrophobic membrane spanning regions 3 3 14 9 [Reporter]SCO3858 (14C2)_PCR/5334 [Gene]SCO3858/4863 NC_003888 SCH69.28c, unknown, len: 258aa; similar to many eg. TR:O53434 (EMBL:AL021897) hypothetical protein from Mycobacterium tuberculosis (262 aa) fasta scores; opt: 837, z-score: 979.2, E(): 0, (50.2% identity in 257 aa overlap). Pfam match to entry PF01255 UPF0015, Uncharacterized protein family UPF0015 3 3 14 8 [Reporter]SCO0526 (14O22)_PCR/5333 [Gene]SCO0526/4862 NC_003888 SCF11.06, possible oxidoreductase, len: 277 aa; similar to TR:O53622 (EMBL:AL021428) Mycobacterium tuberculosis putative oxidoreductase MTV030.21c, 276 aa; fasta scores: opt: 276 z-score: 329.4 E(): 5.6e-11; 24.7% identity in 267 aa overlap and to SW:PRXC_PSEFL (EMBL:AF031153) Pseudomonas fluorescens non-heme chloroperodixase (EC 1.11.1.10) (chloride peroxidase) (CPO-F) (chloropeorixidase F) Cpo or CpoF; fasta scores: opt: 233 z-score: 279.3 E():3.5e-08; 26.1% identity in 283 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 2 2 20 14 [Reporter]SCO5399 (9N11)_PCR/2951 [Gene]SCO5399/2689 NC_003888 SC8F4.03, thiL, probable acetoacetyl-coA thiolase, len: 401aa; similar to many eg. SW:P14611 (THIL_ALCEU) acetoacetyl-coA thiolase from Alcaligenes eutrophus (393 aa) fasta scores; opt: 1272, z-score: 1344.2, E(): 0, 53.6% identity in 390 aa overlap. Also similar to TR:Q9XAM9 (EMBL:AL079355) beta-ketoadipyl-coA thiolase from Streptomyces coelicolor (cosmid 4C6) (400 aa) fasta scores; opt: 949, z-score: 919.8, E(): 0, 42.0% identity in 393 aa overlap. Contains Pfam match to entry PF00108 thiolase and Prosite matches to PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiolases signature 2 and PS00099 Thiolases active site. 3 3 14 7 [Reporter]SCO0089 (15K22)_PCR/5332 [Gene]SCO0089/4861 NC_003888 SCJ11.18, probable LysR-family transcriptional regulator, len: 345 aa; similar to many e.g. SW:ALSR_BACSU ALS operon regulator from Bacillus subtilis (302 aa) fasta scores; opt: 507, z-score: 564.5, E(): 4.4e-24, (31.0% identity in 290 aa overlap). Note possible alternative start codons downstream. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and Prosite match to PS00044 Bacterial regulatory proteins, lysR family signature. 2 2 20 13 [Reporter]SCO2577 (10J11)_PCR/2950 [Gene]SCO2577/2688 NC_003888 SCC123.15c, unknown, len: 148 aa. Highly similar to several proteins of undefined function including: Bacillus subtilis SW:YQEL_BACSU(EMBL:D84432) YqeL (118 aa), fasta scores opt: 286 z-score: 365.1 E(): 6e-13 42.6% identity in 108 aa overlap and Mycobacterium tuberculosis TR:O86327(EMBL:Z81368) (126 aa), fasta scores opt: 441 z-score: 553.8 E(): 1.9e-23 51.9% identity in 129 aa overlap 3 3 14 6 [Reporter]SCO0110 (16G22)_PCR/5331 [Gene]SCO0110/4860 NC_003888 SCJ11.39c, possible DNA-binding protein, len: 279 aa; similar to TR:O86721 (EMBL:AL031515) hypothetical protein from Streptomyces coelicolor (272 aa) fasta scores; opt: 123, z-score: 148.4, E(): 0.66, (26.6% identity in 278 aa overlap). Contains possible helix-turn-helix motif 40-61 aa (+3.21 SD) 3 1 3 5 [Reporter]SCO3892 (17G12)_PCR/1056 [Gene]SCO3892/969 NC_003888 SCH24.14c, sigT, probable RNA polymerase sigma factor, len: 236 aa; previously sequenced as TR:O86856 (EMBL:AJ007313), SigT. Similar to other S.coelicolor putative sigma factors TR:O54163 (EMBL:AL021411) possible sigma factor (200 aa) (32.2% identity in 199 aa overlap) and SCH10.35 putative RNA polymerase sigma factor from cosmid StH10 (294 aa) (37.7% identity in 159 aa overlap). Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily), score 57.10, E-value 3.8e-13 2 2 20 12 [Reporter]SCO2599 (11F11)_PCR/2949 [Gene]SCO2599/2687 NC_003888 SCC88.10c, hypothetical protein, len: 1340 aa; N-terminal region similar to TR:P71905 (EMBL:Z81451;) Mycobacterium tuberculosis hypothetical 103.4 kD protein, 953 aa; fasta scores: opt: 1098 z-score: 829.0 E(): 0; 48.7% identity in 881 aa overlap and C-terminal region similar to SW:CAFA_ECOLI (EMBL:X57166) Escherichia coli ribonuclease G, cytoplasmic axial filament protein CafA, 488 aa; fasta scores: opt: 996 z-score: 756.6 E(): 0; 39.0% identity in 405 aa overlap. Contains match to Prosite entry PS00107 Protein kinases ATP-binding region signature and possible coiled-coil region. Contains also two repeats: 10x(A/T)(T/A)KK and 4x(PEPAAA) 3 1 3 4 [Reporter]SCO3880 (18C12)_PCR/1055 [Gene]SCO3880/968 NC_003888 StH24.02, rpmH, probable 50S ribosomal protein L34, len: 45 aa; previously sequenced as SW:RL34_STRCO (EMBL:M82836), rpmH. Similar to many e.g. SW:RL34_STRBI (EMBL:M83112), rpmH, Streptomyces bikiniensis 50S ribosomal protein L34 (45 aa), fasta scores; opt: 266 z-score: 448.4 E(): 1.2e-17, 91.1% identity in 45 aa overlap. Contains Pfam match to entry PF00468 Ribosomal_L34, Ribosomal protein L34, score 75.20, E-value 1.2e-20 and PS00784 Ribosomal protein L34 signature 2 2 20 11 [Reporter]SCO4173 (12B11)_PCR/2948 [Gene]SCO4173/2686 NC_003888 SCD66.10c, unknown, len: 115 aa 3 1 3 3 [Reporter]SCP1.227c (19O8)_PCR/1054 [Gene]SCP1.227c/967 NC_003888 SCP1.227c, unknown, len: 233aa; previously sequenced and annotated as TR:Q9JN74 (EMBL:AJ276673). Similar to TR:Q9KZ23 (EMBL:AL355752) putative reductase from Streptomyces coelicolor (366 aa) fasta scores; opt: 496, z-score: 569.0, E(): 3.2e-24, 38.2% identity in 212 aa overlap. 2 2 20 10 [Reporter]SCO3283 (13N7)_PCR/2947 [Gene]SCO3283/2685 NC_003888 SCE39.33c, possible precorrin-6X reductase, len: 255 aa; similar to many e.g. SW:COBK_RHOSO precorrin-6X reductase from Rhodococcus sp. (248 aa) fasta scores; opt: 873, z-score: 978.5, E(): 0, (55.1% identity in 247 aa overlap) and SW:COBK_MYCTU precorrin-6X reductase from Mycobacterium tuberculosis (244 aa) fasta scores; opt: 690, z-score: 775.5, E(): 0, (50.4% identity in 244 aa overlap) 3 1 2 22 [Reporter]SCO5362 (1G4)_PCR/1051 [Gene]SCO5362/966 NC_003888 2SC6G5.06, conserved hypothetical protein, len: 281aa; similar to many eg. SW:Q10618 (YD01_MYCTU) hypothetical protein from Mycobacterium tuberculosis (217 aa) fasta scores; opt: 777, z-score: 892.2, E(): 0, 56.7% identity in 208 aa overlap. Contains Pfam match to entry PF01300 Sua5_yciO_yrdC, SUA5/yciO/yrdC family and Prosite match to PS01147 SUA5/yciO/yrdC family signature. 2 2 20 9 [Reporter]SCO0139 (14J7)_PCR/2946 [Gene]SCO0139/2684 NC_003888 SCJ33.03, unknown, len: 106 aa. 3 1 2 20 [Reporter]SCO4603 (2O24)_PCR/1049 [Gene]SCO4603/965 NC_003888 SCD39.03, nuoI2, NADH dehydrogenase subunit, len: 197 aa; similar to SW:NQO9_THETH (EMBL:U52917) Thermus aquaticus NADH-ubiquinone oxidoreductase chain 9 (EC 1.6.5.3) Nqo9, 182 aa; fasta scores: opt: 263 z-score: 292.1 E(): 8.4e-09; 34.9% identity in 146 aa overlap and to TR:Q9XAR2 (EMBL:AL078618) Streptomyces coelicolor SCD16A.13c NuoI, NADH dehydrogenase subunit, 211 aa; fasta scores: opt: 295 z-score: 305.4 E(): 2.3e-11; 34.9% identity in 166 aa overlap. Contains 2x Pfam matches to entry PF00037 fer4, 4Fe-4S binding domain and 2x matches to Prosite entry PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature 2 2 20 8 [Reporter]SCO0390 (15F7)_PCR/2945 [Gene]SCO0390/2683 NC_003888 SCF62.16, possible membrane protein, len: 410 aa. Contains a possible N-terminal signal sequence and multiple possible membrane spanning hydrophobic domains. 3 1 2 19 [Reporter]SCO6148 (3K24)_PCR/1048 [Gene]SCO6148/964 NC_003888 SC1A9.12, unknown, len: 442 aa 2 2 20 7 [Reporter]SCO0722 (16B7)_PCR/2944 [Gene]SCO0722/2682 NC_003888 SCF42.32, possible hydrolase, len: 246 aa. Similar to several proteins of undefined function including: Mycobacterium tuberculosis SW:Y09T_MYCTU (EMBL:Z77165) (262 aa), fasta scores opt: 888 z-score: 1047.3 E():0 56.3% identity in 245 aa overlap and Mycobacterium leprae SW:YY00_MYCLE (EMBL:U00015) (261 aa) fasta scores opt: 843 z-score: 994.7 E():0 51.6% identity in 248 aa overlap. Contains a Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase. 3 1 2 18 [Reporter]SCO6205 (4G24)_PCR/1047 [Gene]SCO6205/963 NC_003888 SC2G5.26c, probable dehydrogenase, len: 296aa; similar to many eg. TR:D1035162 (EMBL:AB015439) D-threonine dehydrogenase (292 aa) fasta scores; opt: 628, z-score:702.7, E(): 7.4e-32, (37.6% identity in 290 aa overlap). 2 2 20 6 [Reporter]SCO3632 (17N3)_PCR/2943 [Gene]SCO3632/2681 NC_003888 SCH10.10c, hypothetical protein, len: 152 aa; unknown function, weakly similar to TR:O53786 (EMBL:AL021943) Mycobacterium tuberculosis hypothetical protein (165 aa), fasta scores; opt: 151 z-score: 200.3 E(): 0.00078, 22.5% identity in 142 aa overlap. Contains probable helix-turn-helix motif at aa 37-58 (Score 982, +2.53 SD) 3 1 2 17 [Reporter]SCO6558 (5C24)_PCR/1046 [Gene]SCO6558/962 NC_003888 SC4B5.08c, protein associated with oxidoreductase activity, len: 282aa; similar to several eg. SW:FDHD_ECOLI FdhD protein from E. coli required for formate dehydrogenase activity at the posttranslational level, mechanism of action unknown (277 aa), fasta scores; opt: 337, z-score: 614.8, E(): 6e-27, (33.7% identity in 267 aa overlap) and SW:NARQ_BACSU NarQ protein from Bacillus subtilis required for nitrate assimilation mechanism of action unknown (262 aa) fasta scores; opt: 320, z-score: 501.0, E(): 1.3e-20, (32.7% identity in 272 aa overlap). 2 2 20 5 [Reporter]SCO0759 (18J3)_PCR/2942 [Gene]SCO0759/2680 NC_003888 SCF81.18, unknown, len: 343 aa 3 1 2 16 [Reporter]SCO7309 (6O20)_PCR/1045 [Gene]SCO7309/961 NC_003888 SC5F8.19, possible membrane transport protein, len: 444 aa. Similar to many including: Streptococcus pyogenes TR:P95827(EMBL:U70055) macrolide-efflux protein, MefA (405 aa), fasta scores opt: 339 z-score: 368.8 E(): 4.5e-13 23.1% identity in 399 aa overlap and Streptomyces coelicolor TR:Q9RKR4(EMBL:AL133220) putative integral membrane protein, SCC75A.10 (431 aa), fasta scores opt: 895 z-score: 962.2 E(): 0 38.2% identity in 408 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 3 1 2 15 [Reporter]SCO4420 (7K20)_PCR/1044 [Gene]SCO4420/960 NC_003888 SC6F11.18, unknown, len: 103 aa. Contains 6x conserved repeat: MR 3 3 14 5 [Reporter]SCO0075 (17C22)_PCR/5330 [Gene]SCO0075/4859 NC_003888 SCJ11.04, unknown, len: 195 aa; weakly similar to SW:KILB_STRLI KilB protein from Streptomyces lividans plasmid pIJ101 thought to be involved in plasmid transfer (147 aa) fasta scores; opt: 126, z-score: 152.9, E(): 0.37, (27.5% identity in 142 aa overlap). Contains probable coiled-coil region approx. 25-45aa. Contains TTA encode leucine, possible site for bldA regulation. 3 3 14 4 [Reporter]SCO6324 (18O18)_PCR/5329 [Gene]SCO6324/4858 NC_003888 SCIF3.26, possible hydrolase, len: 236 aa; similar to TR:CAC13072 (EMBL:AL445503) Streptomyces coelicolor putative hydrolase 2SCG38.13, 238 aa; fasta scores: opt: 320 z-score: 385.7 E(): 6e-14; 34.3% identity in 210 aa overlap. Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase abd a TTA leucine codon, possible target for bldA regulation 3 3 14 3 [Reporter]SCO1861 (19K18)_PCR/5328 [Gene]SCO1861/4857 NC_003888 SCI39.08c, conserved hypothetical protein, len: 363 aa; similar to SW:YT15_MYCTU (EMBL:Z74697) Mycobacterium tuberculosis hypothetical 39.8 kDa protein RV2915c, 370 aa; fasta scores: opt: 1341 Z-score: 1487.0 bits: 283.8 E(): 3.1e-75; 56.630% identity in 362 aa overlap 3 3 14 2 [Reporter]SCO7470 (20C18)_PCR/5327 [Gene]SCO7470/4856 NC_003888 SCBAC17A6.03c, possible phenylacetic acid degradation protein PaaI, len: 170 aa: similar to many e.g. SW:P76084 (PAAI_ECOLI) phenylacetic acid degradation protein PaaI from Escherichia coli (140 aa) fasta scores; opt: 342, Z-score: 414.6, 45.902% identity (46.281% ungapped) in 122 aa overlap. Contains Pfam match to entry PF02584 DUF157, Uncharacterized protein PaaI. 3 3 13 22 [Reporter]SCO1288 (1C14)_PCR/5325 [Gene]SCO1288/4855 NC_003888 2SCG18.35c, possible integral membrane protein, len: 168 aa. Contains possible hydrophobic membrane spanning regions 3 3 13 21 [Reporter]SCO4067 (2O10)_PCR/5324 [Gene]SCO4067/4854 NC_003888 SCD25.03, dnaZ, DNA polymerase III subunit gamma, len: 784 aa. Highly similar to the gamma subunit of Mycobacterium tuberculosis SW:DP3X_MYCTU (EMBL; AL022121) (EC 2.7.7.7) DNA polymerase III subunit gamma/tau (578 aa), fasta scores opt: 1757 z-score: 1081.7 E(): 0 59.5% identity in 435 aa overlap. Note that after residue ~433 the C-terminus of this protein has no significant database similarities. Contains a PS00017 ATP/GTP-binding site motif A (P-loop). 3 3 13 20 [Reporter]SCO5905 (3K10)_PCR/5323 [Gene]SCO5905/4853 NC_003888 SC10A5.10c, probable export protein, len: 847 aa; similar to eg. S. coelicolor actinorhodin antibiotic export protein TR:Q53902 (EMBL:M64683) ORF2 (ACTII) (711 aa), fasta scores; opt: 705 z-score: 1126.2 E(): 0, 35.4% identity in 746 aa overlap 3 3 13 19 [Reporter]SCO5555 (4G10)_PCR/5322 [Gene]SCO5555/4852 NC_003888 SC1C2.36, unknown, len: 76 aa 2 2 20 4 [Reporter]SCP1.237c (19F3)_PCR/2941 [Gene]SCP1.237c/2679 NC_003888 SCP1.237c, mmr, methylenomycin A resistance protein, len: 475aa; previously sequenced and annotated as hypothetical protein SW:P11545 (MMR_STRCO) (EMBL:AJ276673). Similar to many others eg. SW:P39886 (TCMA_STRGA) tetracenomycin C resistance and export protein from Streptomyces glaucescens (538 aa) fasta scores; opt: 797, z-score: 882.3, E(): 0, 32.7% identity in 425 aa overlap. Contains multiple possible membrane-spanning hydrophobic regions. 3 3 13 18 [Reporter]SCO6411 (5C10)_PCR/5321 [Gene]SCO6411/4851 NC_003888 SC3C8.30, probable hydrolase, len: 280 aa; similar to e.g. BUP_RAT beta-ureidopropionase (EC 3.5.1.6) (beta-alanine synthase) (393 aa), fasta scores; opt: 529 z-score: 700.2 E(): 9.2e-32, 37.2% identity in 296 aa overlap. Also highly similar to TR:E1292351 (EMBL:AL023496) putative hydrolase SC1A6.03, fasta scores; E(): 0, 72.1% identity in 280 aa overlap 2 2 20 3 [Reporter]SCO6310 (20B3)_PCR/2940 [Gene]SCO6310/2678 NC_003888 SCIF3.12, probable cytochrome P450, len: 447 aa; similar to SW:CPXG_STRSQ (EMBL:M31939) Streptomyces sp. cytochrome P450 105C1 (EC 1.14.-.-) ChoP, 381 aa; fasta scores: opt: 301 z-score: 318.8 E(): 3.2e-10; 28.8% identity in 386 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450 3 3 13 17 [Reporter]SCO5826 (6O6)_PCR/5320 [Gene]SCO5826/4850 NC_003888 SC5B8.16, putative membrane protein, len: 192 aa. Contains possible hydrophobic membrane spanning regions 3 1 2 14 [Reporter]SCO6883 (8G20)_PCR/1043 [Gene]SCO6883/959 NC_003888 SC7F9.35c, possible membrane protein, len: 104 aa. Highly similar to Streptomyces coelicolor TR:Q9X868 (EMBL:AL049661) hypothetical 10.3 KD protein, SCE134.13 (102 aa), fasta scores: opt: 543 z-score: 619.4 E(): 4.4e-27 80.0% identity in 100 aa overlap. Contains possible membrane spanning hydrophobic domains 2 2 20 2 [Reporter]SCO7703 (20J23)_PCR/2939 [Gene]SCO7703/2677 NC_003888 SCBAC12C8.04, possible integral membrane transport protein, len: 416aa: similar to many eg. TR:BAB35923 (EMBL:AP002558) putative amino acid/amine transport protein from Escherichia coli O157:H7 (393 aa) fasta scores; opt: 953, Z-score: 1012.0, 41.207% identity (41.645% ungapped) in 381 aa overlap. Contains Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and multiple possible membrane-spanning hydrophobic regions. 3 1 2 13 [Reporter]SCO7161 (9C20)_PCR/1042 [Gene]SCO7161/958 NC_003888 SC9A4.23c, probable 3-oxoacyl-[acyl-carrier protein] reductase, len: 260 aa; similar to SW:FABG_ECOLI (EMBL:M84991) Escherichia coli 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) FabG, 244 aa; fasta scores: opt: 453 z-score: 474.7 E(): 5.6e-19; 34.4% identity in 247 aa overlap and to TR:Q9RJV4 (EMBL:AL117387) Streptomyces coelicolor probable oxidoreductase SCF41.15, 263 aa; fasta scores: opt: 1092 z-score: 881.9 E(): 0; 72.5% identity in 251 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature. Contains possible hydrophobic membrane spanning region 2 2 19 22 [Reporter]SCO1346 (1J19)_PCR/2937 [Gene]SCO1346/2676 NC_003888 2SCG61.28c, fabG3, possible 3-oxoacyl-[acyl-carrier protein] reductase, len: 253 aa; similar to many eg. SW:P25716 (FABG_ECOLI) 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) from Escherichia coli (244 aa) fasta scores; opt: 624, z-score: 674.0, E(): 4.5e-30, 40.5% identity in 247 aa overlap. Contains Pfam match to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus, Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature. 3 1 2 12 [Reporter]SCO1946 (10O16)_PCR/1041 [Gene]SCO1946/957 NC_003888 SCC54.06c, pgk, phosphoglycerate kinase, len: 403 aa; member of a family of proteins highly conserved across prokaryotes and eukaryotes, e.g. SW:PGK_BACSU Pgk, phosphoglycerate kinase from Bacillus subtilis (394 aa) fasta scores; opt: 1263, z-score: 1322.3, E(): 0, (51.2% identity in 404 aa overlap) and SW:PGKH_SPIOL phosphoglycerate kinase from Spinacia oleracea (Spinach) (433 aa) fasta scores; opt: 1200, z-score: 1256.1, E(): 0, (49.9% identity in 407 aa overlap). Contains PS00111 Phosphoglycerate kinase signature and Pfam match to entry PF00162 PGK, Phosphoglycerate kinases, score 654.10, E-value 7.1e-193. 2 2 19 21 [Reporter]SCO6500 (2F19)_PCR/2936 [Gene]SCO6500/2675 NC_003888 SC1E6.09, gvpA, probable gas vesicle synthesis protein, len: 144 aa; highly similar in N-terminus to many e.g. GVPA_PSEAN gas vesicle protein (71 aa), fasta scores; opt: 219 z-score: 363.2 E(): 6e-13, 65.4% identity in 52 aa overlap. Contains PS00234 Gas vesicles protein GVPa signature 1 and Pfam match to entry PF00741 Gas_vesicle, Gas vesicle protein, score 44.20, E-value 2.9e-09 3 1 2 11 [Reporter]SCO4515 (11K16)_PCR/1040 [Gene]SCO4515/956 NC_003888 SCD35.22c, hypothetical protein, len: 401 aa; similar to TR:Q9XD98 (EMBL:AF106004) Streptomyces coelicolor hypothetical 27.5 kD protein, 249 aa; fasta scores: opt: 190 z-score: 190.8 E(): 0.0034; 26.8% identity in 190 aa overlap and to TR:Q9F3G6 (EMBL:AL392175) Streptomyces coelicolor putative membrane protein 2SCD4.05, 474 aa; fasta scores: opt: 583 Z-score: 556.4 E(): 2.3e-23; 36.422% identity in 313 aa overlap. Contains possible hydrophobic membrane spanning regions and also possible colied-coil region ar aprox. residues 104..127 2 2 19 20 [Reporter]SCO7654 (3B19)_PCR/2935 [Gene]SCO7654/2674 NC_003888 SC10F4.27, possible oxidoreductase, len: 285 aa; similar to many other dehydrogenases/oxidoreductases, e.g. TR:Q9PCP9 (EMBL:AE003996) Xylella fastidiosa phenylacetaldehyde dehydrogenase XF1729, 291 aa; fasta scores: opt: 626 z-score: 722.9 E(): 9.9e-33; 39.7% identity in 292 aa overlap 3 1 2 10 [Reporter]SCO4272 (12G16)_PCR/1039 [Gene]SCO4272/955 NC_003888 SCD95A.05, possible mutT-like protein, len: 323 aa; similar to TR:O69888 (EMBL:AL023797) Streptomyces coelicolor hypothetical 19.4 kD protein SC2E1.17 or MutT, 172 aa; fasta scores: opt: 266 z-score: 322.5 E(): 1.7e-10; 39.3% identity in 145 aa overlap. Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein and match to Prosite entry PS00893 mutT domain signature. High content in alanine and leucine amino acid residues 2 2 19 19 [Reporter]SCO7219 (4N15)_PCR/2934 [Gene]SCO7219/2673 NC_003888 SC2H12.18c, possible phosphoglycerate mutase, len: 208 aa. Similar to Deinococcus radiodurans TR:Q9RUJ3(EMBL:AE001985) phosphoglycerate mutase (237 aa), fasta scores opt: 143 z-score: 182.9 E(): 0.01 31.5% identity in 222 aa overlap. Contains 2xPfam matches to entry PF00300 PGAM, Phosphoglycerate mutase family. 3 1 2 9 [Reporter]SCO5140 (13C16)_PCR/1038 [Gene]SCO5140/954 NC_003888 SCP8.03, hypothetical protein, len: 252 aa; similar to TR:Q46063 (EMBL:X81379) Corynebacterium glutamicum ORF2, 211 aa; fasta scores: opt: 938 z-score: 1070.3 E(): 0; 67.1% identity in 210 aa overlap 2 2 19 18 [Reporter]SCO7681 (5J15)_PCR/2933 [Gene]SCO7681/2672 NC_003888 SC4C2.16c, probable AMP-binding ligase, len: 553 aa; similar to SW:DHBE_BACSU (EMBL:U26444) Bacillus subtilis 2,3-dihydroxybenzoate-AMP ligase (EC 6.3.2.-) DhbE, 539 aa; fasta scores: opt: 1324 z-score: 1411.8 E(): 0; 51.6% identity in 539 aa overlap. Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and match to Prosite entry PS00455 Putative AMP-binding domain signature 3 1 2 8 [Reporter]SCO0473 (14O12)_PCR/1037 [Gene]SCO0473/953 NC_003888 SCF76.13c, possible solute-binding lipoprotein, len: 329 aa. Similar to many e.g. Streptococcus parasanguis SW:ADHS_STRPA (EMBL; M26130) adhesin B precursor (saliva-binding protein) (309 aa), fasta scores opt: 354 z-score: 399.1 E(): 7.2e-15 23.7% identity in 317 aa overlap. Contains an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site, a Pfam match to entry PF01297 Lipoprotein_4, Adhesion lipoprotein and a possible N-terminal signal sequence. 2 2 19 17 [Reporter]SCO7773 (6F15)_PCR/2932 [Gene]SCO7773/2671 NC_003888 SC5E9.21c, conserved hypothetical protein, len: 174aa; weakly similar to many eg. TR:Q00818 (EMBL:M95287) hypothetical protein from plasmid IncN R46 of Salmonella typhimurium (162 aa) fasta scores; opt: 188, z-score: 238.9, E(): 7.7e-06, 29.3% identity in 157 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 3 1 2 7 [Reporter]SCO3853 (15K12)_PCR/1036 [Gene]SCO3853/952 NC_003888 SCH69.23c, possible membrane protein, len: 237 aa; similar to TR:P71582 (EMBL:Z80233) hypothetical protein from Mycobacterium tuberculosis (262 aa) fasta scores; opt: 506, z-score: 588.6, E(): 1.9e-25, (39.8% identity in 236 aa overlap). Contains possible membrane spanning hydrophobic region at the N-terminus. 2 2 19 16 [Reporter]SCO6167 (7B15)_PCR/2931 [Gene]SCO6167/2670 NC_003888 SC6C5.03, proline rich protein (putative membrane protein), len: 456 aa. Contains possible hydrophobic membrane spanning regions 3 1 2 6 [Reporter]SCO0523 (16G12)_PCR/1035 [Gene]SCO0523/951 NC_003888 SCF11.03c, probable transcriptional regulator, len: 145 aa; similar to SW:ASNC_ECOLI (EMBL:AE000451) Escherichia coli, regulatory protein AsnC, 152 aa; fasta scores: opt: 222 z-score: 287.1 E(): 1.3e-08; 26.9% identity in 145 aa overlap. Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family 3 1 2 5 [Reporter]SCO5146 (17C12)_PCR/1034 [Gene]SCO5146/950 NC_003888 SCP8.09c, probable methyltransferase, len: 174 aa; similar to SW:MDMC_STRMY (EMBL:M93958) Streptomyces mycarofaciens O-methyltransferase (EC 2.1.1.-) MdmC, 221 aa; fasta scores: opt: 253 z-score: 313.8 E(): 5.2e-10; 31.5% identity in 178 aa overlap. Contains Pfam match to entry PF01596 Methyltransf_3, O-methyltransferase 3 3 13 16 [Reporter]SCO5439 (7K6)_PCR/5319 [Gene]SCO5439/4849 NC_003888 SC6A11.15, hypothetical protein, len: 755 aa; similar to TR:O32215 (EMBL:Z99121) hypothetical protein from Bacillus subtilis (774 aa) fasta scores: opt: 323, z-score: 361.2, E(): 1e-12, 23.5% identity in 763 aa overlap 3 3 13 15 [Reporter]SCO4755 (8G6)_PCR/5318 [Gene]SCO4755/4848 NC_003888 SC6G4.33, possible transcriptional regulator, len: 360 aa; similar to many putative membrane bound regulators e.g. TR:P96499 (EMBL:U56901) B. subtilis putative transcriptional regulator (391 aa), fasta scores; opt: 333 z-score: 2 78.7 E(): 2.9e-08, 28.1% identity in 352 aa overlap. Also similar to SC1C3.08c (E(): 0, 52.7% identity in 334 aa overlap. Contains possible transmembrane domain near N-terminus 3 3 13 14 [Reporter]SCO7730 (9C6)_PCR/5317 [Gene]SCO7730/4847 NC_003888 SC8D11.21, conserved hypothetical protein, len: 112 aa; similar to TR:Q9RTC1 (EMBL:AE002024) Deinococcus radiodurans conserved hypothetical protein DR1844, 93 aa; fasta scores: opt: 259 z-score: 352.8 E(): 4.1e-12; 42.4% identity in 85 aa overlap 3 3 13 13 [Reporter]SCO6261 (10O2)_PCR/5316 [Gene]SCO6261/4846 NC_003888 SCAH10.26, probable secreted protein, len: 169 aa; similar to TR:Q9F1W0 (EMBL:AB040071) Streptomyces griseus secreted protein, 198 aa; fasta scores: opt: 647 Z-score: 783.2 E(): 5.5e-36; 55.030% identity in 169 aa overlap. Contains possible N-terminal region signal sequence 3 3 13 12 [Reporter]SCO2355 (11K2)_PCR/5315 [Gene]SCO2355/4845 NC_003888 SCC8A.13, possible lipoprotein, len: 256aa; contains an appropriately positioned Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site 3 3 13 11 [Reporter]SCO4770 (12G2)_PCR/5314 [Gene]SCO4770/4844 NC_003888 SCD63.02, guaB, inosine 5' monophosphate dehydrogenase, len: 501 aa; highly similar to previously sequenced TR:O86844 (EMBL:AJ010601) Streptomyces coelicolor inosine 5' monophosphate dehydrogenase, 523 aa; fasta scores: opt: 2626 z-score: 2896.3 E(): 0; 84.9% identity in 509 aa overlap. Contains Pfam matches to entries PF01574 IMPDH_N, IMP dehydrogenase / GMP reductase N terminus, 2x PF00571 CBS, CBS domain and PF00478 IMPDH_C, IMP dehydrogenase / GMP reductase C terminus and match to Prosite entry PS00487 IMP dehydrogenase / GMP reductase signature 3 3 13 10 [Reporter]SCO2861 (13C2)_PCR/5313 [Gene]SCO2861/4843 NC_003888 SCE20.35, unknown, len: 240 aa. 3 3 13 9 [Reporter]SCO0826 (13O22)_PCR/5312 [Gene]SCO0826/4842 NC_003888 SCF43A.16, hypothetical protein, len: 266 aa; unknown function, shares short internal regions of similarity with several methyltransferases e.g. TR:O82434 (EMBL:AF053766) Nicotiana tabacum S-adenosyl-methionine cycloartenol-C24-methyltransferase (349 aa), fasta scores; opt: 165 z-score: 206.0 E(): 0.00038, 29.8% identity in 104 aa overlap. Weakly similar to part of SC1E6.19C (EMBL:AL033505) S.coelicolor hypothetical protein (273 aa) (35.1% identity in 131 aa overlap) 2 2 19 15 [Reporter]SCO5750 (8N11)_PCR/2930 [Gene]SCO5750/2669 NC_003888 SC7C7.05, ftsK homolog, integral membrane protein, len: 929; similar to members of the ftsK/spoIIIE family invoilved in cell division, e.g. ftsK/spoIIIE SP3E_BACSU stage iii sporulation protein E (787 aa), fasta scores; opt: 1525 z-score: 1355.4 E(): 0, 45.1% identity in 552 aa overlapand FTSK_ECOLI cell division protein ftsK (1329 aa), fasta scores; opt: 717 z-score: 1180.9 E(): 0, 37.1% identity in 701 aa overlap. Contains 7 degenerate repeats of AKKA(A/P) near N-terminus and PS00017 ATP/GTP-binding site motif A (P-loop). Contains possible hydrophobic membrane spanning regions 3 3 13 8 [Reporter]SCO0843 (14K22)_PCR/5311 [Gene]SCO0843/4841 NC_003888 SCF43A.33c, unknown, len: 144 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 2 2 19 14 [Reporter]SCO7009 (9J11)_PCR/2929 [Gene]SCO7009/2668 NC_003888 SC8F11.35c, unknown, len: 593 aa. Identical, apart from the very C-terminus, to Streptomyces lividans TR:O86875(EMBL:U22894) hypothetical 57.9 kd protein, AglX (547 aa), fasta scores opt: 3357 z-score: 3741.0 E(): 0 98.8% identity in 519 aa overlap. Also highly similar to several Streptomyces coelicolor hypothetical proteins e.g. TR:CAB61537(EMBL:AL133171) SCF81.26 (835 aa), fasta scores opt: 1133 z-score: 1260.6 E(): 0 43.0% identity in 575 aa overlap. 3 3 13 7 [Reporter]SCO3404 (15G22)_PCR/5310 [Gene]SCO3404/4840 NC_003888 SCE9.11c, ftsH2, cell division protein ftsH homolog, len: 668 aa; similar to many e.g. SW:FTSH_ECOLI (EMBL:M83138), ftsH, Escherichia coli membrane-bound, ATP-dependent protease involved in cell cycle control (644 aa), fasta scores; opt: 1921 z-score: 1987.4 E(): 0, 48.5% identity in 641 aa overlap. Similar to TR:O69875 (EMBL:AL023797), ftsH, S.coelicolor cell division protein ftsH homolog (648 aa) (43.3% identity in 610 aa overlap), but lacking the proline-rich N-terminus. Contains a hydrophobic, possible membrane-spanning region near the N-terminus. Contains Pfam match to entry PF00004 AAA, ATPases associated with various cellular activities (AAA), score 335.00, E-value 8.4e-97, PS00674 AAA-protein family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 3 1 2 4 [Reporter]SCO0891 (18O8)_PCR/1033 [Gene]SCO0891/949 NC_003888 SCM1.24, possible DNA-binding protein, len: 279 aa. Highly similar to many Streptomyces coelicolor putative DNA-binding proteins e.g. TR:CAB53273 (EMBL:AL109972) SCJ9A.12C (279 aa), fasta scores opt: 564 z-score: 639.5 E(): 2.8e-28 37.2% identity in 269 aa overlap. Contains a Pfam match to entry PF01381 HTH_3, Helix-turn-helix and a Prosite hit to PS00092 N-6 Adenine-specific DNA methylases signature. The putative helix-turn-helix motif is situated between residues 35..56 (+4.25 SD) 2 2 19 13 [Reporter]SCO3800 (10F11)_PCR/2928 [Gene]SCO3800/2667 NC_003888 SCGD3.01, possible acyl-CoA dehydrogenase, partial CDS, len: >491 aa; weakly similar to TR:P97088 (EMBL:Z92974), bcd, Clostridium thermosaccharolyticum butyryl-CoA dehydrogenase (379 aa), fasta scores; opt: 176 z-score: 203.7 E(): 0.00051, 28.0% identity in 318 aa overlap. Also highly similar to putative acyl-CoA dehydrogenases from mycobacteria e.g. TR:O69506 (EMBL:AL022486) Mycobacterium leprae probable acyl-CoA dehydrogenase (611 aa) (64.3% identity in 493 aa overlap). Contains two Pfam matches to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase,SCAC2.08, possible dehydrogenase (fragment), len: >149 aa; similar to N-terminal region of TR:O53666 (EMBL:AL021929;) Mycobacterium tuberculosis putative acyl-CoA dehydrogenase MTV034.10c, 611 aa; fasta scores: opt: 547 z-score: 662.5 E(): 2.1e-29; 52.4% identity in 147 aa overlap 3 1 2 3 [Reporter]SCO1678 (19K8)_PCR/1032 [Gene]SCO1678/948 NC_003888 SCI52.20c, probable transcriptional regulator, len: 233 aa; similar to TR:O50536 (EMBL:AJ003022) Streptomyces coelicolor sporulation transcriptional factor WhiH, 295 aa; fasta scores: opt: 432 Z-score: 525.2 E(): 1.1e-21; 34.404% identity in 218 aa overlap and to SW:DGOR_ECOLI (EMBL:L10328) Escherichia coli galactonate operon transcriptional repressor DgoR or B3694/B3695, 229 aa; fasta scores: opt: 320 Z-score: 393.0 E(): 2.6e-14; 29.808% identity in 208 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and possible helix-turn-helix motif at residues 31..52 (+3.18 SD) 2 2 19 12 [Reporter]SCO2591 (11B11)_PCR/2927 [Gene]SCO2591/2666 NC_003888 SCC88.02. possible secreted protein, len: 576 aa; similar to TR:CAB51964 (EMBL:AL109661) Streptomyces coelicolor hypothetical 100.7 kD protein SC6E10.10, 943 aa; fasta scores: opt: 522 z-score: 556.2 E(): 1.4e-23; 31.8% identity in 471 aa overlap. Contains possible N-terminal region signal peptide sequence 3 1 2 2 [Reporter]SCP1.322 (20C8)_PCR/1031 [Gene]SCP1.322/947 NC_003888 SCP1.322, unknown, len: 126aa; 2 2 19 11 [Reporter]SCO4630 (12N7)_PCR/2926 [Gene]SCO4630/2665 NC_003888 SCD82.01c, unknown (fragment), len: >275 aa,SCD39.30c, hypothetical protein (fragment), len: >179 aa. Low G+C content (53.78%) 3 1 1 22 [Reporter]SCO5203 (1C4)_PCR/1029 [Gene]SCO5203/946 NC_003888 2SC3B6.27c, unknown, len: 223 aa. Contains two TTA leucine codons, possible targets for bldA regulation 2 2 19 10 [Reporter]SCO3279 (13J7)_PCR/2925 [Gene]SCO3279/2664 NC_003888 SCE39.29, possible integral membrane efflux protein, len: 404aa; similar to many eg. SW:KEFB_ECOLI glutathione-regulated potassium-efflux system protein, KefB (601 aa) fasta scores; opt: 250, z-score: 293.4, E(): 5e-09, (27.1% identity in 414 aa overlap). Contains several potential membrane spanning hydrophobic regions. 3 1 1 21 [Reporter]SCO4917 (1O24)_PCR/1028 [Gene]SCO4917/945 NC_003888 SCK13.09c, probable purine nucleoside phosphorylase, len: 274 aa; similar to SW:PUNA_BACST (EMBL:D87959) Bacillus stearothermophilus purine nucleoside phosphorylase I (EC 2.4.2.1) PunA, 274 aa; fasta scores: opt: 354 z-score: 414.8 E(): 1.4e-15; 43.4% identity in 249 aa overlap. Contains Pfam match to entry PF00896 Mtap_PNP, phosphorylases family 2 2 2 19 9 [Reporter]SCO1026 (14F7)_PCR/2924 [Gene]SCO1026/2663 NC_003888 SCG20A.06, probable transcriptional regulatory protein, len: 274 aa; similar to SW:AGAR_ECOLI (EMBL:U18997) Escherichia coli putative aga operon transcriptional repressor AgaR, 269 aa; fasta scores: opt: 524 z-score: 597.5 E(): 8.4e-26; 36.6% identity in 246 aa overlap. Contains Pfam match to entry PF00455 deoR, Bacterial regulatory proteins, deoR family and match to Prosite entry PS00894 Bacterial regulatory proteins, deoR family signature. Contains also possible helix-turn-helix motif at residues 26..47 (+2.94 SD) 3 1 1 20 [Reporter]SCO4568 (2K24)_PCR/1027 [Gene]SCO4568/944 NC_003888 SCD16A.15c, nuoG, NADH dehydrogenase subunit, len: 843 aa; similar to many e.g. TR:P95173 (EMBL:Z83867) NuoG, NADH dehydrogenase subunit from Mycobacterium tuberculosis (806 aa) fasta scores; opt: 1968, z-score: 2051.4, E(): 0, (62.5% identity in 818 aa overlap) and SW:NUOG_ECOLI, NADH dehydrogenase subunit NuoG from Escherichia coli (180 aa) fasta scores; opt: 394, z-score: 474.3, E(): 4.3e-19, (39.3% identity in 163 aa overlap). Contains Pfam matches to entry PF00384 molybdopterin, Prokaryotic molybdopterin oxidoreductases and to entry PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains. Also contains Prosite match to PS00642 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. 2 2 19 8 [Reporter]SCO1068 (15B7)_PCR/2923 [Gene]SCO1068/2662 NC_003888 SCG22.14, conserved hypothetical protein, len: 246 aa; similar to TR:CAB92209 (EMBL:AL356592) Streptomyces coelicolor hypothetical 27.6 kD protein SC9H11.20, 255 aa; fasta scores: opt: 369 z-score: 455.9 E(): 6.4e-18; 37.0% identity in 257 aa overlap 3 1 1 19 [Reporter]SCO6792 (3G24)_PCR/1026 [Gene]SCO6792/943 NC_003888 SC1A2.01, possible TetR-family transcriptional regulator, len: 198 aa. Weakly similar to Escherichia coli SW:UIDR_ECOLI (EMBL:M14641) UID operon repressor (GUS operon repressor) (196 aa), fasta scores opt: 164 z-score: 213.3 E(): 0.00018 24.7% identity in 186 aa overlap. Also similar to Streptomyces coelicolor TR:CAB61186 (EMBL:AL132973) putative TetR-family transcriptional regulator, SCF91.29 (205 aa), fasta scores opt: 265 z-score: 338.0 E(): 2e-11 34.1% identity in 185 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 33..54 (+5.25 SD). 2 2 19 7 [Reporter]SCO1641 (16N3)_PCR/2922 [Gene]SCO1641/2661 NC_003888 SCI41.24c, probable transport protein, len: 405 aa; identical to TR:O87601 (EMBL:AF086832) S. coelicolor putative transporter (419 aa), and similar to e.g. TR:O30996 (EMBL:AF024666) Corynebacterium striatum R-plasmid pTP10 chloramphenicol export protein (391 aa), fasta scores; opt: 194 z-score: 210.6 E(): 0.00023, 25.9% identity in 394 aa overlap 3 1 1 18 [Reporter]SCO6201 (4C24)_PCR/1025 [Gene]SCO6201/942 NC_003888 SC2G5.22, gcl, probable glyoxylate carboligase, acetohydroxy acid synthase-pyruvate oxidase family,len:594aa; highly similar to SW:GCL_ECOLI glyoxylate carboligase from Escherichia coli (592 aa) fasta scores;opt: 2905, z-score: 3262. 8,E(): 0, (72.7% identity in 586aa overlap). Contains Pfam match to entry PF00205TPP_enzymes, Thiamine pyrophosphate enzymes, score 427.80, E-value 4.7e-132. Note: as with the Escherichia coli homologue, this CDS is located close to a glyoxylate induced protein of unknown function. 2 2 19 6 [Reporter]SCO1455 (17J3)_PCR/2921 [Gene]SCO1455/2660 NC_003888 SCL6.12c, possible hydrolase, len: 262 aa; similar to TR:O69808 (EMBL:AL023496) Streptomyces coelicolor putative hydrolase SC1A6.03, 280 aa; fasta scores: opt: 389 z-score: 454.5 E(): 6.8e-18; 31.2% identity in 276 aa overlap. Contains Pfam match to entry PF00795 CN_hydrolase, Carbon-nitrogen hydrolase and match to Prosite entry PS01227 Uncharacterized protein family UPF0012 signature 3 1 1 17 [Reporter]SCO7046 (5O20)_PCR/1024 [Gene]SCO7046/941 NC_003888 SC4G1.12, probable integral membrane protein, len: 124 aa; similar to SW:CRCB_ECOLI (EMBL:D28497) Escherichia coli CrcB protein, 127 aa; fasta scores: opt: 181 z-score: 232.0 E(): 1.9e-05; 34.5% identity in 113 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 1 15 [Reporter]SCO7414 (7G20)_PCR/1022 [Gene]SCO7414/940 NC_003888 SC6D11.10, possible integral membrane protein, len: 268 aa. Contains possible membrane spanning hydrophobic domains. 3 3 13 6 [Reporter]SCO1235 (16C22)_PCR/5309 [Gene]SCO1235/4839 NC_003888 2SCG1.10c, ureB, urease beta subunit, len: 103 aa; similar to SW:URE2_RHIME (EMBL:S69145) Rhizobium meliloti urease beta subunit UreB (EC 3.5.1.5), 101 aa; fasta scores: opt: 400 z-score: 525.2 E(): 8.7e-22; 59.8% identity in 102 aa overlap. Contains Pfam match to entry PF00699 Urease_beta, Urease beta subunit 3 3 13 5 [Reporter]SCO1565 (17O18)_PCR/5308 [Gene]SCO1565/4838 NC_003888 SCL24.01c, probable glycerophosphoryl diester phosphodiesterase (fragment), len: >291 aa; similar to SW:GLPQ_ECOLI (EMBL:X56907) Escherichia coli glycerophosphoryl diester phosphodiesterase, periplasmic precursor (EC 3.1.4.46) GlpQ, 358 aa; fasta scores: opt: 321 z-score: 336.9 E(): 2.4e-11; 35.1% identity in 228 aa overlap. Contains possible N-terminal region signal peptide sequence,SCL11.21c, probable glycerophophosdyl diester phosphodiesterase (fragment), len: >133 aa; similar to SW:GLPQ_ECOLI (EMBL:X56907) Escherichia coli glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46) GlpQ, 358 aa; fasta scores: opt: 146 z-score: 185.0 E(): 0.0069; 34.3% identity in 102 aa overlap 3 3 13 4 [Reporter]SCP1.285c (18K18)_PCR/5307 [Gene]SCP1.285c/4837 NC_003888 SCP1.285c, possible conserved secreted protein, len: 299aa; similar to many eg. TR:Q9XAF8 (EMBL:AL079356) hypothetical protein from Streptomyces coelicolor (365 aa) fasta scores; opt: 999, z-score: 1101.9, E(): 0, 52.8% identity in 303 aa overlap. Contains a possible cleavable N-terminal signal sequence 3 3 13 3 [Reporter]SCO6001 (19G18)_PCR/5306 [Gene]SCO6001/4836 NC_003888 SCBAC1C11.04, putative membrane protein, len: 95 aa. Contains possible hydrophobic membrane spanning region 3 3 13 2 [Reporter]SCO5977 (20O14)_PCR/5305 [Gene]SCO5977/4835 NC_003888 SCBAC16H6.12, possible amino acid permease, len: 479aa: similar to many eg. SW:O43246 (CTR4_HUMAN) cationic amino acid permease from Homo sapiens (636 aa) fasta scores; opt: 704, Z-score: 760.9, 33.679% identity (34.946% ungapped) in 386 aa overlap and TR:Q9L100 (EMBL:AL159139) putative amino acid permease SCL6.16c from Streptomyces coelicolor (496 aa) fasta scores; opt: 1575, Z-score: 1699.3, 53.377% identity (53.493% ungapped) in 459 aa overlap. Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease and possible membrane-spanning hydrophobic regions. 3 3 12 22 [Reporter]SCO1259 (1O10)_PCR/5303 [Gene]SCO1259/4834 NC_003888 2SCG18.06, probable two-component system sensor kinase, len: 384 aa; similar to TR:Q9ZBU9 (EMBL:AL034446) Streptomyces coelicolor putative sensor kinase SC1A9.03, 388 aa; fasta scores: opt: 377 z-score: 409.6 E(): 2.5e-15; 32.5% identity in 381 aa overlap and to TR:Q9Z678 (EMBL:AF102271) Streptomyces coelicolor sensor-histidine kinase, 398 aa; fasta scores: opt: 454 z-score: 491.8 E(): 6.6e-20; 38.0% identity in 287 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 12 21 [Reporter]SCO2468 (2K10)_PCR/5302 [Gene]SCO2468/4833 NC_003888 SC7A8.07c, dnaG, DNA primase, len: 641 aa; identical to previously sequenced TR:CAB51551 (EMBL:AJ244019) Streptomyces coelicolor A3(2) DNA primase DnaG, 641 aa and similar to SW:PRIM_MYCTU (EMBL:Z83860) Mycobacterium tuberculosis DNA primase (EC 2.7.7.-) DnaG, 639 aa; fasta scores: opt: 1854 z-score: 2053.6 E(): 0; 47.6% identity in 634 aa overlap. Contains Pfam match to entry PF01807 zf-CHC2, CHC2 zinc finger and to entry PF01751 Toprim, Toprim domain 3 3 12 20 [Reporter]SCO5423 (3G10)_PCR/5301 [Gene]SCO5423/4832 NC_003888 SC8F4.27c, pyk2, pyruvate kinase, len: 476 aa; strongly similar to many e.g. SW:Q46078 (KPYK_CORGL) pyruvate kinase from Corynebacterium glutamicum (Brevibacterium flavum) (475 aa) fasta scores; opt: 1693, z-score: 1907.7, E(): 0, 55.3% identity in 474 aa overlap and TRNEW:CAB52070 (EMBL:AL109732) pyruvate kinase from Streptomyces coelicolor (478 aa) fasta scores; opt: 2185, z-score: 2461.2, E(): 0, 69.3% identity in 473 aa overlap. Contains Pfam match to entry PF00224 PK, Pyruvate kinase and Prosite match to PS00110 Pyruvate kinase active site signature. 2 2 19 5 [Reporter]SCO0941 (18F3)_PCR/2920 [Gene]SCO0941/2659 NC_003888 SCM10.29c, possible lipoprotein, len: 384 aa; weakly similar to SW:CAPA_BACAN (EMBL:M24150) Bacillus anthracis CapA protein, 411 aa; fasta scores: opt: 270 z-score: 296.6 E(): 4e-09; 24.0% identity in 338 aa overlap. Contains match correctly situated to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal signal peptide sequence 3 3 12 19 [Reporter]SCO5549 (4C10)_PCR/5300 [Gene]SCO5549/4831 NC_003888 SC1C2.30, probable hydrolase, len: 244 aa; similar to hypothetical proteins from many organisms and to HAD1_PSESP 2-haloalkanoic acid dehalogenase I (EC 3.8. (227 aa), fasta scores; opt: 201 z-score: 261.1 E(): 2.7e-07, 30.4% identity in 237 aa overlap. Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase, score 43.50, E-value 4.8e-09 2 2 19 4 [Reporter]SCP1.07c (19B3)_PCR/2919 [Gene]SCP1.07c/2658 NC_003888 SCP1.07c, unknown, len: 97aa; 3 3 12 18 [Reporter]SCO2200 (5O6)_PCR/5299 [Gene]SCO2200/4830 NC_003888 SC3H12.08c, hypothetical protein, len: 279 aa; identical to N-terminal region of previously sequenced TR:CAB58282 (EMBL:AL121850) Streptomyces coelicolor conserved hypothetical protein SCC78.01c (fragment), 234 aa, identical to C-terminal region of TR:CAB51284 (EMBLAL096872:) Streptomyces coelicolor hypothetical 9.2 kD protein SC5F7.01c (fragment), 85 aa and similar to TR:O30485 (EMBL:AF007101) Streptomyces hygroscopicus hypothetical 29.8 kD protein, 282 aa; fasta scores: opt: 559 z-score: 634.1 E(): 6.1e-28; 40.6% identity in 286 aa overlap 3 1 1 14 [Reporter]SCO6879 (8C20)_PCR/1021 [Gene]SCO6879/939 NC_003888 SC7F9.31c, hypothetical protein, len: 153 aa. Similar to Streptomyces coelicolor TR:Q9X872(EMBL:AL049661) hypothetical 16.3 KD protein, SCE134.17 (150 aa), fasta scores opt:309 z-score: 375.4 E(): 1.7e-13 38.6% identity in 153 aa overlap. 2 2 19 3 [Reporter]SCP1.312 (19N23)_PCR/2918 [Gene]SCP1.312/2657 NC_003888 SCP1.312, possible TetR-family regulator, len: 215aa; similar to many eg. TR:Q9L2D0 (EMBL:AL137187) putative TetR-family transcriptional regulator from Streptomyces coelicolor (194 aa) fasta scores; opt: 317, z-score: 374.6, E(): 2.1e-13, 34.5% identity in 206 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. 3 1 1 13 [Reporter]SCO6127 (9O16)_PCR/1020 [Gene]SCO6127/938 NC_003888 SC9B2.14, probable carboxylesterase, len: 502aa; similar to many both prokaryotic and eukaryotic eg. SW:PCD_ARTOX phenylcarbamate hydrolase, Pcd, from Arthrobacter oxidans (493 aa) fasta scores; opt: 924, z-score: 897.3, E(): 0, (37.2% identity in 503 aa overlap). Contains PS00122 Carboxylesterases type-B serine active site and 2x Pfam match to entry PF00135 COesterase, Carboxylesterases. 2 2 19 2 [Reporter]SCO5994 (20F23)_PCR/2917 [Gene]SCO5994/2656 NC_003888 SCBAC16H6.29, possible integral membrane cytochrome biogenesis protein, len: 300 aa; similar to TR:Q9R6T1 (EMBL:AF079137) CcdA from Synechococcus sp. (strain PCC 6301) (252 aa) fasta scores; opt: 245, Z-score: 273.8, 30.208% identity (33.721% ungapped) in 192 aa overlap. Contains Pfam match to entry PF02683 DsbD, Cytochrome C biogenesis protein transmembrane region and possible membrane-spanning hydrophobic regions 3 1 1 12 [Reporter]SCO1942 (10K16)_PCR/1019 [Gene]SCO1942/937 NC_003888 SCC54.02c, pgi2, glucose-6-phosphate isomerase, len: 551 aa; Member of family of proteins conserved across prokaryotes and eukaryotes. Almost identical to another from Streptomyces coelicolor TR:O88015 (EMBL:AL031107) pgi, glucose-6-phosphate isomerase (550 aa) fasta scores; opt: 3305, z-score: 3849.4, E(): 0, (91.9% identity in 542 aa overlap). Also similar to SW:G6PI_ECOLI pgi, glucose-6-phosphate isomerase from Escherichia coli (549 aa) fasta scores; opt: 1950, z-score: 2271.0, E(): 0, (54.6% identity in 548 aa overlap) and SW:G6PI_MOUSE gpi, glucose-6-phosphate isomerase from Mus musculus (mouse) (557 aa) fasta scores; opt: 1901, z-score: 2213.9, E(): 0, (52.1% identity in 557 aa overlap). Contains PS00174 Phosphoglucose isomerase signature 2 and Pfam match to entry PF00342 PGI, Phosphoglucose isomerase, score 903.80, E-value 4.9e-268. 2 2 18 22 [Reporter]SCO1124 (1F19)_PCR/2915 [Gene]SCO1124/2655 NC_003888 2SCG38.17c, possible integral membrane protein, len: 163 aa; similar to TR:O31623 (EMBL:Z99110) Bacillus subtilis YjcA protein, 118 aa; fasta scores: opt: 229 z-score: 294.7 E(): 5.7e-09; 38.2% identity in 110 aa overlap. Contains possible hydrophobic membrane spanning regions 3 1 1 11 [Reporter]SCO4350 (11G16)_PCR/1018 [Gene]SCO4350/936 NC_003888 SCD19.05c, possible integrase, len: 475 aa; highly similar to TR:Q9RAJ3 (EMBL:AJ250372) Mycobacterium sp. GP1 putative integrase IntM, 451 aa; fasta scores: opt: 2152 z-score: 2497.3 E(): 0; 72.8% identity in 441 aa overlap 2 2 18 21 [Reporter]SCO3670 (2B19)_PCR/2914 [Gene]SCO3670/2654 NC_003888 SCH44.10c, grpE, heat shock protein, len: 225 aa; identical to previously SW:GRPE_STRCO (EMBL:X77458) Streptomyces coelicolor GrpE protein, 225 aa. Contains Pfam match to entry PF01025 GrpE, GrpE and match to Prosite entry PS01071 grpE protein signature. Contains also possible coiled-coil region at aprox. residues 52..79 3 1 1 10 [Reporter]SCO4259 (12C16)_PCR/1017 [Gene]SCO4259/935 NC_003888 SCD8A.32c, possible AAA family ATPase, len: 690 aa; C-terminal region similar to TR:CAB74213 (EMBL:AL139075) Campylobacter jejuni probable AAA family ATPase CJ0377, 570 aa; fasta scores: opt: 378 z-score: 402.0 E(): 5.8e-15; 28.9% identity in 256 aa overlap and to SW:YB56_METJA (EMBL:U67557) Methanococcus jannaschii cell division cycle protein 48 homolog MJ1156, 903 aa; fasta scores: opt: 278 z-score: 293.2 E(): 6.7e-09; 30.4% identity in 240 aa overlap. Contains Pfam match to entry PF00004 AAA, ATPases associated with various cellular activities (AAA) and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 2 18 20 [Reporter]SCO6817 (3N15)_PCR/2913 [Gene]SCO6817/2653 NC_003888 SC1A2.26c, unknown, len: 119 aa. 3 1 1 9 [Reporter]SCO3457 (13O12)_PCR/1016 [Gene]SCO3457/934 NC_003888 SCE46.14, possible transmembrane protein, len: 205 aa; similar to TR:P76226 (EMBL:AE000270) Escherichia coli from bases 1830199 to 1840304, 208 aa; fasta scores: opt: 402 z-score: 471.0 E(): 6.9e-19; 37.4% identity in 206 aa overlap and to TR:CAB50482 (EMBL:AJ248288) Pyrococcus abyssi predicted CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase PgsA, 185 aa; fasta scores: opt: 216 z-score: 259.1 E(): 4.4e-07; 31.2% identity in 205 aa overlap 2 2 18 19 [Reporter]SCO7215 (4J15)_PCR/2912 [Gene]SCO7215/2652 NC_003888 SC2H12.14c, hypothetical protein, len: 186 aa. Similar to Deinococcus radiodurans TR:Q9RRF8(EMBL:AE002082) hypothetical protein, GrpB (188 aa), fasta scores opt: 281 z-score: 356.9 E(): 2.1e-12 35.9% identity in 184 aa overlap and to SW:YI88_BACHD (EMBL:AP001513) Bacillus halodurans hypothetical protein BH1888, 195 aa; fasta scores: opt: 471 Z-score: 557.2 E(): 2.1e-23; 41.361% identity in 191 aa overlap 3 1 1 8 [Reporter]SCO0165 (14K12)_PCR/1015 [Gene]SCO0165/933 NC_003888 SCJ1.14c, unknown, len:137 aa, shares a low level of similarity with Aquifex aeolicus TR:O66857 (EMBL:AE000697) hypothetical 14.5 KD protein (130 aa), fasta scores opt:144 z-score: 190.2 E(): 0.003127.2% identity in 103 aa overlap. Contains a PS00216 Sugar transport proteins signature 1. 2 2 18 18 [Reporter]SCO2392 (5F15)_PCR/2911 [Gene]SCO2392/2651 NC_003888 SC4A7.20, possible lipoprotein, len: 305 aa; similar to TR:Q9X7W9 (EMBL:AL049485) Streptomyces coelicolor hypothetical 28.1 kD protein, 261 aa; fasta scores: opt: 137 z-score: 160.4 E(): 0.15; 31.0% identity in 187 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 3 1 1 7 [Reporter]SCO0241 (15G12)_PCR/1014 [Gene]SCO0241/932 NC_003888 SCJ9A.20, possible transcriptional regulator, len: 224aa; similar to many proposed transcriptional regulators eg. TR:O53737 (EMBL:AL021932) putative transcriptional regulator from Streptomyces coelicolor (236 aa) fasta scores; opt: 308, z-score: 371.4, E(): 2.5e-13, (30.3% identity in 221 aa overlap). Contains possible helix-turn-helix motif (+3.83 SD) 37-58 aa 2 2 18 17 [Reporter]SCO7768 (6B15)_PCR/2910 [Gene]SCO7768/2650 NC_003888 SC5E9.16, conserved hypothetical protein, len: 146aa; similar to SW:O31511 (YESE_BACSU) hypothetical protein from Bacillus subtilis (147 aa) fasta scores; opt: 317, z-score: 427.2, E(): 2.5e-16, 35.7% identity in 126 aa overlap. 3 1 1 6 [Reporter]SCO0512 (16C12)_PCR/1013 [Gene]SCO0512/931 NC_003888 SCF6.08c, possible regulatory protein, len: 202 aa. Similar to many other putative regulatory proteins e.g. Streptomyces coelicolor TR:CAB53122 (EMBL; AL109962) putative transcriptional regulatory protein SCJ1.04 (207 aa), fasta scores opt: 436 z-score: 515.3 E(): 2.5e-21 44.4% identity in 196 aa overlap. 3 1 1 5 [Reporter]SCO1533 (17O8)_PCR/1012 [Gene]SCO1533/930 NC_003888 SCL2.23c, unknown, len: 188 aa 3 3 12 17 [Reporter]SCO5821 (6K6)_PCR/5298 [Gene]SCO5821/4829 NC_003888 SC5B8.11, probable serine protease, len: 285 aa; similar to many members of the trypsin family e.g. TRYP_SACERP 24664 from saccharopolyspora erythraea (streptomyces erythraeus) (227 aa), fasta scores; opt: 523 z-score: 655.1 E(): 2.7e-29, 44.7% identity in 237 aa overlap. Contains PS00134 Serine proteases, trypsin family, histidine active site, PS00135 Serine proteases, trypsin family, serine active site and Pfam match to entry trypsin PF00089, Trypsin, score 228.48 3 3 12 14 [Reporter]SCO2654 (9O2)_PCR/5295 [Gene]SCO2654/4828 NC_003888 SC8E4A.24, hypothetical protein, len: 194 aa; similar to various hypothetical proteins, e.g. TR:CAB56360 (EMBL:AL118514) Streptomyces coelicolor hypothetical 21.6 kD protein SCD25.16c, 194 aa; fasta scores: opt: 700 z-score: 857.1 E(): 0; 58.5% identity in 188 aa overlap 3 3 12 13 [Reporter]SCO6257 (10K2)_PCR/5294 [Gene]SCO6257/4827 NC_003888 SCAH10.22, probable ABC transport system sugar binding lipoprotein, len: 334 aa; similar to TR:CAB41563 (EMBL:AL049727) Streptomyces coelicolor putative secreted solute binding protein, 337 aa; fasta scores: opt: 1003 z-score: 1130.9 E():0; 49.8% identity in 325 aa overlap, to SW:RBSB_BACSU (EMBL:Z92953) Bacillus subtilis D-ribose-binding protein precursor RbsB, 305 aa; fasta scores: opt: 206 z-score: 238.7 E(): 6.3e-06; 23.0% identity in 235 aa overlap and to TR:CAB41563 (EMBL:SC9B1) Streptomyces coelicolor SC9B1.17c, 337 aa; fasta scores: opt: 1003 z-score: 1045.2 E(): 0; 49.8% identity in 325 aa overlap. Contains match to Pfam entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family and a match situated in the correct position to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide sequence 3 3 12 12 [Reporter]SCO2255 (11G2)_PCR/5293 [Gene]SCO2255/4826 NC_003888 SCC75A.01c, possible membrane protein, partial CDS, len: > 268 aa. Highly similar to another putative membrane protein from Streptomyces coelicolor TR:CAB45459(EMBL:AL079348) SC66T3.03 (330 aa), fasta scores opt: 695 z-score: 823.4 E():0 54.8% identity in 241 aa overlap. Contains possible membrane spanning hydrophobic domains.,SC1G2.17c, unknown, partial CDS, len: > 116 aa. Highly similar to the C-terminus of Streptomyces coelicolor TR:Q9XAL0(EMBL:AL079348) putative membrane protein SC66T3.03 (330 aa), fasta scores opt: 472 z-score: 600.5 E(): 4.6e-26 66.1% identity in 109 aa overlap. 3 3 12 11 [Reporter]SCO4455 (12C2)_PCR/5292 [Gene]SCO4455/4825 NC_003888 SCD6.33, possible integral membrane protein, len: 187 aa; similar to TR:Q98DK3 (EMBL:AP003004) MLR4665 protein from Rhizobium loti (Mesorhizobium loti) fasta scores; opt: 419, Z-score: 476.1, E(): 6.9e-19, 43.373% identity (44.172% ungapped) in 166 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 12 10 [Reporter]SCO2830 (12O22)_PCR/5291 [Gene]SCO2830/4824 NC_003888 SCE20.04, probable amino acid ABC transporter protein, integral membrane component, len: 216 aa. Highly similar to several ABC transporter permeases including: Escherichia coli SW:GLNP_ECOLI(EMBL:X14180) glutamine transport system permease protein GlnP (219 aa), fasta scores opt: 378 z-score: 454.8 E(): 6e-18 33.2% identity in 217 aa overlap and Rhizobium sp. (strain NGR234) SW:Y4TG_RHISN(EMBL:AE000098) probable amino-acid ABC transporter permease protein Y4TG (231 aa), fasta scores opt: 667 z-score: 792.8 E(): 0 47.6% identity in 208 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. 3 3 12 9 [Reporter]SCO0855 (13K22)_PCR/5290 [Gene]SCO0855/4823 NC_003888 SCM2.08c, possible acetyltransferase, len: 168 aa. Weak similarity to several other putative acetyltransferases e.g. Streptomyces coelicolor TR:CAB51273 (EMBL:AL096872) putative acetyltransferase SC5F7.12C (169 aa), fasta scores opt: 121 z-score: 155.1 E(): 0.29 31.3% identity in 147 aa overlap. Contains a Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 3 3 12 8 [Reporter]SCO0700 (14G22)_PCR/5289 [Gene]SCO0700/4822 NC_003888 SCF42.10c, probable ABC transporter protein, ATP-binding component, len: 589 aa. The N-terminus is identical to the previously sequenced Streptomyces coelicolor TR:Q53899(EMBL:X60316) pleiotropic regulatory locus AbaA, OrfC (fragment)(>131 aa), fasta scores opt: 856 z-score: 951.7 E(): 0 100.0% identity in 131 aa overlap. Also highly similar to many including: Escherichia coli SW:MSBA_ECOLI(EMBL:Z11796) probable transport ATP-binding protein, MsbA (582 aa), fasta scores opt: 875 z-score: 963.0 E():0 31.4% identity in 566 aa overlap and Deinococcus radiodurans TR:AAF12435(EMBL:AE001863) ABC transporter, ATP-binding protein (602 aa), fasta scores opt: 941 z-score: 1035.3 E():0 34.4% identity in 593 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and Pfam matches to entries PF00005 ABC_tran, ABC transporter and PF00664 ABC_membrane, ABC transporter transmembrane region. Also contains Prosite hits to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 2 2 18 16 [Reporter]SCO5659 (7N11)_PCR/2909 [Gene]SCO5659/2649 NC_003888 SC6A9.08c, possible integral membrane protein, len: 179 aa; contains four possible transmembrane domains 3 3 12 7 [Reporter]SCO3821 (15C22)_PCR/5288 [Gene]SCO3821/4821 NC_003888 SCGD3.22, pksC, serine/threonine protein kinase, len: 556 aa; previously sequenced as (EMBL:AF094711), PksC, Streptomyces coelicolor serine/threonine protein kinase (556 aa). Highly similar to SCGD3.21c which lies upstream, on the opposite strand. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain, PS00108 Serine/Threonine protein kinases active-site signature and PS00107 Protein kinases ATP-binding region signature 2 2 18 15 [Reporter]SCO5746 (8J11)_PCR/2908 [Gene]SCO5746/2648 NC_003888 SC7C7.01, unknown, len: 225 aa; some similarity to hydrophobic region from B. subtilis DEGT_BACST pleiotropic regulatory protein (372 aa), fasta scores; opt: 289 z-score: 450.7 E(): 7.2e-18, 51.7% identity in 89 aa overlap. Also similar to e.g. TR:P72452 (EMBL:Y00459) aminotransferase from Streptomyces griseus(378 aa), fasta scores; opt: 250 z-score: 429.3 E(): 1.1e-16, 29.4% identity in 309 aa overlap 3 3 12 6 [Reporter]SCO0528 (16O18)_PCR/5287 [Gene]SCO0528/4820 NC_003888 SCF11.08, possible lipoprotein, len: 261 aa; similar to various hypothetical proteins, e.g. TR:O07236 (EMBL:Z96800) Mycobacterium tuberculosis hypothetical 22.5 KD protein, 218 aa; fasta scores: opt: 157 z-score: 172.7 E(): 0.03; 25.3% identity in 194 aa overlap. Contains a Prosite match correctly situated to entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains probable N-terminal signal sequence 3 1 1 3 [Reporter]SCP1.104 (19G8)_PCR/1010 [Gene]SCP1.104/929 NC_003888 SCP1.104, unknown, len: 154aa; weakly similar to SW:P71686 (YE17_MYCTU) fasta scores; opt: 113, z-score: 145.5, E(): 1.2, 33.6% identity in 107 aa overlap. 2 2 18 14 [Reporter]SCO5518 (9F11)_PCR/2907 [Gene]SCO5518/2647 NC_003888 SC8D9.30c, possible transcriptional regulator, len: 521 aa; some similarity to TR:Q00509 (EMBL:X63451) SrmR, regulatory gene governing the expression of a polyketide synthase gene in Streptomyces ambofaciens (604 aa) fasta scores; opt: 142, z-score: 152.0, E(): 0.37, (28.1% identity in 228 aa overlap). Also similar to TR:E1359977 (EMBL:AL034492) putative transcriptional regulator from Streptomyces coelicolor (569 aa) fasta scores; opt: 204, z-score: 216.7, E(): 9.1e-05, (28.6% identity in 448 aa overlap). Contains possible helix-turn-helix at 367-388 aa (+2.83 SD). 2 1 23 22 [Reporter]SCO4922 (1J24)_PCR/1008 [Gene]SCO4922/928 NC_003888 SCK13.14, possible integral membrane protein, len: 160 aa. Contains 4 possible hydrophobic membrane spanning regions 2 2 18 13 [Reporter]SCO3796 (10B11)_PCR/2906 [Gene]SCO3796/2646 NC_003888 SCAC2.04, hypothetical protein, len: 722 aa; similar to TR:Q9RIW0 (EMBL:AL133278) Streptomyces coelicolor hypothetical 64.1 kDa protein (fragment) SCM11.01c, 588 aa; fasta scores: opt: 1053 z-score: 1177.1 E(): 0; 35.8% identity in 598 aa overlap 2 1 23 21 [Reporter]SCO4800 (2F24)_PCR/1007 [Gene]SCO4800/927 NC_003888 SCD63A.11, icmB, isobutyryl CoA mutase, small subunit, len: 138 aa; almost identical to TR:CAB59633 (EMBL:AJ246005) Streptomyces cinnamonensis isobutyryl CoA mutase, small subunit (EC 5.4.99.13) IcmB, 136 aa; fasta scores: opt: 803 z-score: 972.5 E(): 0; 92.5% identity in 134 aa overlap and highly similar to TR:CAB71846 (EMBL:138667) Streptomyces coelicolor probable isobutyryl-CoA mutase, small subunit SC3D9.01, 159 aa; fasta scores: opt: 594 z-score: 628.1 E(): 2e-29; 70.6% identity in 126 aa overlap 2 2 18 12 [Reporter]SCO4390 (11N7)_PCR/2905 [Gene]SCO4390/2645 NC_003888 SCD10.22, hypothetical protein, len: 467 aa; similar to TR:CAB92197 (EMBL:AL356592) Streptomyces coelicolor hypothetical 84.7 kD protein SC9H11.08, 802 aa; fasta scores: opt: 261 z-score: 286.0 E(): 1.9e-08; 29.2% identity in 463 aa overlap. Contains 2 Pfam matches to entry PF00989 PAS, PAS domain and Pfam match to entry PF00785 PAC, PAC motif 2 1 23 20 [Reporter]SCO6328 (3B24)_PCR/1006 [Gene]SCO6328/926 NC_003888 SC10H5.04c, putative membrane protein, len: 103 aa. Contains possible hydrophobic membrane spanning regions 2 2 18 11 [Reporter]SCO3960 (12J7)_PCR/2904 [Gene]SCO3960/2644 NC_003888 SCD78.27c, unknown, len: 280 aa, similar to family of hypothetical proteins e.g. TR:O07810 (EMBL:Z97188) hypothetical 28.5 kDa protein from Mycobacterium tuberculosis (273 aa), fasta scores; opt: 597, z-score: 413.5, E(): 8.7e-164, 3.2% identity in 266 aa overlap. Also similar to SCD78.17c approx 9 kb downstream (37.9% identity in 285 aa overlap). Contains Pfam match to entry PF00592 DUF3, Domain of Unknown Function 3, score 96.90, E-value 4e-25 2 1 23 19 [Reporter]SCO6221 (4N20)_PCR/1005 [Gene]SCO6221/925 NC_003888 SC2H4.03, possible lipoprotein, len: 153 aa; contains possible N-terminal signal sequence with appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 2 2 18 10 [Reporter]SCO3083 (13F7)_PCR/2903 [Gene]SCO3083/2643 NC_003888 SCE25.24c, possible integral membrane protein, len: 307 aa; highly similar to TR:Q54192 (EMBL:M80628) Streptomyces griseus transfer hypothetical product, 293 aa; fasta scores: opt: 1265 z-score: 1184.1 E(): 0; 70.7% identity in 307 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 23 18 [Reporter]SCO7040 (5J20)_PCR/1004 [Gene]SCO7040/924 NC_003888 SC4G1.06c, gap2, glyceraldehyde-3-phosphate dehydrogenase, len: 481 aa; highly similar to TR:O68923 (EMBL:AF058302) Streptomyces roseofulvus glyceraldehyde-3-phosphate dehydrogenase homolog GapX, 461 aa; fasta scores: opt: 2562 z-score: 2921.8 E(): 0; 84.5% identity in 459 aa overlap and to many eukaryotic homologos, e.g. SW:G3PA_MAIZE (EMBL:X15408) Zea mays glyceraldehyde-3-phosphate dehydrogenase A, chloroplast precursor (EC 1.2.1.12) GapA, 403 aa; fasta scores: opt: 793 z-score: 907.5 E(): 0; 39.6% identity in 366 aa overlap. Contains Pfam match to entry PF00044 gpdh, glyceraldehyde 3-phosphate dehydrogenases and match to Prosite entry PS00071 Glyceraldehyde 3-phosphate dehydrogenase active site 2 2 18 8 [Reporter]SCO0717 (15N3)_PCR/2901 [Gene]SCO0717/2642 NC_003888 SCF42.27, possible integral membrane protein, len: 375 aa. Similar to several other membrane proteins from Streptomyces coelicolor e.g. TR:CAB56388(EMBL:AL118515) SCD17.11 (454 aa), fasta scores opt: 569 z-score: 648.9 E(): 9.3e-29 36.4% identity in 360 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 2 1 23 17 [Reporter]SCO7572 (6F20)_PCR/1003 [Gene]SCO7572/923 NC_003888 SC5F1.26, possible oxidoreductase, len: 998 aa; similar to TR:Q9KKW5 (EMBL:AE004425) Vibrio cholerae oxidoreductase/iron-sulfur cluster-binding protein VCA0985, 959 aa; fasta scores: opt: 1999 z-score: 2189.0 E(): 0; 35.3% identity in 966 aa overlap. Contains Pfam matches to entries PF01565 FAD_binding_4, FAD binding domain and PF00037 fer4, 4Fe-4S binding domain and matches to Prosite entries PS00422 Granins signature 1 and PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature 2 2 18 7 [Reporter]SCO3867 (16J3)_PCR/2900 [Gene]SCO3867/2641 NC_003888 SCH18.04c, soyB1, probable ferredoxin, len: 70 aa; similar to SW:FERS_STRGR (EMBL:X63601) Streptomyces griseus ferredoxin Soy, SoyB, 65 aa; fasta scores: opt: 169 z-score: 239.4 E(): 7.3e-06; 44.4% identity in 63 aa ovelap 2 1 23 16 [Reporter]SCO7409 (7B20)_PCR/1002 [Gene]SCO7409/922 NC_003888 SC6D11.05c, possible binding-protein dependent transport protein, len: 316 aa. Similar to many including: Streptomyces coelicolor TR:CAB61179(EMBL:AL132973) putative binding protein dependent transport protein, SCF91.22 (287 aa), fasta scores opt: 875 z-score: 1002.5 E(): 0 45.9% identity in 281 aa overlap. Contains possible membrane spanning hydrophobic domains and a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. 2 2 18 6 [Reporter]SCO5047 (17F3)_PCR/2899 [Gene]SCO5047/2640 NC_003888 SCK7.20c, conserved hypothetical protein, len: 343 aa; similar to SW:GLPX_ECOLI (EMBL:Z11767) Escherichia coli hypothetical protein GlpX, 336 aa; fasta scores: opt: 789 z-score: 868.1 E(): 0; 46.5% identity in 325 aa overlap 2 1 23 15 [Reporter]SCO2011 (8N16)_PCR/1001 [Gene]SCO2011/921 NC_003888 SC7H2.25, probable branched chain amino acid transport ATP-binding protein, len: 309aa; similar to many eg. SW:LIVG_ECOLI branched chain amino acid transport ATP-binding protein from Escherichia coli (255 aa) fasta scores; opt: 770, z-score: 837.9, E(): 0, (46.8% identity in 250 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 2 1 23 14 [Reporter]SCO6121 (9J16)_PCR/1000 [Gene]SCO6121/920 NC_003888 SC9B2.08c, possible transcriptional regulator, len: 192 aa; similar to many e.g. TR:O34892 (EMBL:AF027868) proposed transcriptional regulator from Bacillus subtilis (191 aa) fasta scores; opt: 168, z-score: 207.7, E(): 0.00028, (30.2% identity in 96 aa overlap) and SW:TER3_ECOLI tetracycline repressor protein class C from Escherichia coli (219 aa) fasta scores; opt: 149, z-score: 184.7, E(): 0.0053, (45.5% identity in 55 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Also contains a probable helix-turn-helix (+4.23 SD) at residues 35..56 3 3 12 5 [Reporter]SCO0359 (17K18)_PCR/5286 [Gene]SCO0359/4819 NC_003888 SCF41.18, hypothetical protein, len: 240 aa; similar to TR:Q9KEZ0 (EMBL:AP001509) Bacillus halodurans BH0709 protein, 242 aa; fasta scores: opt: 515 Z-score: 603.7 E(): 5.4e-26; 36.000% identity in 225 aa overlap 3 3 12 4 [Reporter]SCO0894 (18G18)_PCR/5285 [Gene]SCO0894/4818 NC_003888 SCM1.27, possible membrane protein, len: 478 aa. Contains possible membrane spanning hydrophobic domain. 3 3 12 3 [Reporter]SCO1867 (19C18)_PCR/5284 [Gene]SCO1867/4817 NC_003888 SCI39.14, possible hydroxylase, len: 280 aa; similar to TR:O06499 (EMBL:D78338) Dactylosporangium L-proline 4-hydroxylase, 272 aa; fasta scores: opt: 458 Z-score: 529.1 bits: 105.7 E(): 7.1e-22; 34.351% identity in 262 aa overlap 3 3 12 2 [Reporter]SCO5081 (20K14)_PCR/5283 [Gene]SCO5081/4816 NC_003888 SCBAC28G1.07, actVA6, hypothetical protein, len: 113 aa; identical to previously sequenced TR:Q53908 (EMBL:X58833) Streptomyces coelicolor 6 ActVA region genes of the actinorhodin biosynthetic gene cluster, ActVA6, 113 aa and similar to SW:TCMH_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin polyketide synthesis hydroxylase TcmH, 112 aa; fasta scores: opt: 193 Z-score: 247.6 bits: 51.0 E(): 3.5e-06; 43.210% identity in 81 aa overlap 3 3 11 22 [Reporter]SCO1168 (1K10)_PCR/5281 [Gene]SCO1168/4815 NC_003888 2SCG11.02c, hypothetical protein, len: 422 aa; similar to C-terminal region of TR:CAB60182 (EMBL:AL132824) Streptomyces coelicolor hypothetical 81.9 kD protein SCAH10.28, 797 aa; fasta scores: opt: 1009 z-score: 960.4 E(): 0; 44.4% identity in 435 aa overlap 3 3 11 21 [Reporter]SCO1578 (2G10)_PCR/5280 [Gene]SCO1578/4814 NC_003888 SCL24.14c, argB, acetylglutamate kinase, len: 306 aa; highly similar to SW:ARGB_CORGL (EMBL:X86157) Corynebacterium glutamicum acetylglutamate kinase (EC 2.7.2.8) ArgB, 294 aa; fasta scores: opt: 1117 z-score: 1270.1 E(): 0; 62.5% identity in 267 aa overlap. Contains Pfam match to entry PF00696 aakinase, Amino acid kinase family 3 3 11 20 [Reporter]SCO4079 (3C10)_PCR/5279 [Gene]SCO4079/4813 NC_003888 SCD25.15, purL, phosphoribosyl formylglycinamidine synthase II (EC 6.3.5.3), len: 752 aa, Highly similar to many e.g. Mycobacterium tuberculosis SW:PURL_MYCTU (EMBL; U34956) phosphoribosylformylglycinamidine synthase II (EC 6.3.5.3) (FGAM synthase II) (754 aa), fasta scores opt: 3547 z-score: 3844.2 E():0 70.5% identity in 749 aa overlap. Contains 2x Pfam matches to entry PF00586 AIRS, AIR synthase related proteins. 3 3 11 19 [Reporter]SCO6607 (4O6)_PCR/5278 [Gene]SCO6607/4812 NC_003888 SC1F3.04, unknown, len: 261 aa; similar to many Streptomyces coelicolor e.g. upstream gene SC1F2.03c,E(): 0, 48.8% identity in 260 aa overlap 2 2 18 5 [Reporter]SCO1802 (18B3)_PCR/2898 [Gene]SCO1802/2639 NC_003888 SCI33.01c, possible two-component system sensor kinase (fragment), len: >383 aa; similar to N-terminal region of TR:Q9L1F1 (EMBL:AL157916) Streptomyces coelicolor putative two-component system sensor kinase SC3D11.11, 442 aa; fasta scores: opt: 406 z-score: 434.9 E(): 1.1e-16; 38.9% identity in 211 aa overlap. Contains Pfam matches to entries PF01590 GAF, GAF domain and PF02518 HATPase_c, GAF domain,SCI5.10c, partial CDS, possible two component sensor kinase, len: >41aa; similar to the C-terminus of many egs. TR:Q52558 (EMBL:U02041) VsrA, virulence regulator from Ralstonia solanacearum (502 aa) fasta scores; opt: 115, z-score: 182.8, E(): 0.0074, (51.4% identity in 35 aa overlap) and SW:DEGS_BACBR DegS, sensor kinase from Bacillus brevis (386 aa) fasta scores; opt: 111, z-score: 178.6, E(): 0.013, (44.1% identity in 34 aa overlap). 3 3 11 18 [Reporter]SCO2195 (5K6)_PCR/5277 [Gene]SCO2195/4811 NC_003888 SC5F7.06, unknown, len: 71aa; 2 2 18 4 [Reporter]SCO6321 (18N23)_PCR/2897 [Gene]SCO6321/2638 NC_003888 SCIF3.23, possible integral membrane protein, len: 362 aa; highly similar to TR:Q9KZX9 (EMBL:AL353815) Streptomyces coelicolor putative integral membrane protein SCD6.25, 362 aa; fasta scores: opt: 1695 z-score: 1862.4 E(): 0; 73.8% identity in 362 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 11 17 [Reporter]SCO2046 (6G6)_PCR/5276 [Gene]SCO2046/4810 NC_003888 SC4G6.15c, possible integral membrane efflux protein, len: 384aa; similar to many egs. TR:O31137 (EMBL:AF030344) TetV, tetracycline resistance determinant from Mycobacterium smegmatis (419 aa) fasta scores; opt: 446, z-score: 465.6, E(): 1.3e-18, (27.3% identity in 381 aa overlap) and TR:CAB41206 (EMBL:AL049661) putative integral membrane efflux protein from Streptomyces coelicolor (500 aa) fasta scores; opt: 416, z-score: 433.8, E(): 7.7e-17, (32.8% identity in 375 aa overlap). Contains possible membrane spanning hydrophobic regions. 2 1 23 13 [Reporter]SCO2443 (10F16)_PCR/999 [Gene]SCO2443/919 NC_003888 SCC24.14, probable carrier protein (putative membrane protein), len: 465 aa; similar to TR:Q9ZIP4 (EMBL:AF022387) Rhizobium trifolii putative dicarboxylate carrier protein gene MatC, 448 aa; fasta scores: opt: 827 z-score: 814.6 E(): 0; 51.0% identity in 465 aa overlap. Contains possible hydrophobic membrane spanning regions 2 2 18 2 [Reporter]SCO5083 (20B23)_PCR/2895 [Gene]SCO5083/2637 NC_003888 SCBAC28G1.09, actII-2, probable actinorhodin transporter, len: 578 aa; identical to previously sequenced SW:AC22_STRCO (EMBL:M64683) Streptomyces coelicolor probable actinorhodin transporter ActII-2, 578 aa. Contains possible hydrophobic membrane spanning regions and a leucine TTA codon, possible target for bldA regulation 2 1 23 12 [Reporter]SCO4115 (11B16)_PCR/998 [Gene]SCO4115/918 NC_003888 SCD72A.01, unknown, partial CDS, len: >590 aa,SCD17A.07, possible secreted protein (fragment), len: >151 aa. Contains possible and cleavable N-terminal region signal peptide sequence 2 2 17 22 [Reporter]SCO1120 (1B19)_PCR/2893 [Gene]SCO1120/2636 NC_003888 2SCG38.13, probable hydrolase, len: 238 aa; similar to SW:GPHC_ALCEU (EMBL:M68904) Alcaligenes eutrophus phosphoglycolate phosphatase, chromosomal (EC 3.1.3.18) CbbZC, 231 aa; fasta scores: opt: 300 z-score: 354.5 E(): 2.7e-12; 32.9% identity in 207 aa overlap. Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase 2 1 23 11 [Reporter]SCO4228 (12N12)_PCR/997 [Gene]SCO4228/917 NC_003888 SCD8A.01c, probable phosphate transport system regulatory protein (fragment), len: >139 aa; similar to C-terminal region of TR:O53833 (EMBL:AL022004) Mycobacterium tuberculosis phosphate transport system regulatory protein, PhoY-2, 213 aa; fasta scores: opt: 383 z-score: 454.2 E(): 7.2e-18; 46.8% identity in 139 aa overlap and of SW:PHOU_ECOLI (EMBL:X02723) Escherichia coli phosphate transport system regulatory protein, PhoU, 241 aa; fasta scores: opt: 213 z-score: 258.9 E(): 5.5e-07; 28.1% identity in 128 aa overlap,2SCD46.42c, possible regulatory protein, partial CDS, len: >123aa; similar to the C-terminus of many proposed eg. phosphate transport regulators eg. TR:O53833 (EMBL:AL022004) phosphate transport system regulatory protein from Mycobacterium tuberculosis (213 aa) fasta scores; opt: 380, z-score: 494.5, E(): 4.4e-20, 52.9% identity in 104 aa overlap. 2 2 17 21 [Reporter]SCO0713 (2N15)_PCR/2892 [Gene]SCO0713/2635 NC_003888 SCF42.23c, lipA, lipase (putative secreted protein), len: 310 aa. Identical to the previously sequenced and characterised: Streptomyces coelicolor TR:Q9ZIW6(EMBL:AF009336) lipase, LipA (310 aa). Contains a Prosite hit to PS00120 Lipases, serine active site and a possible hydrophobic domain close to the N-terminus 2 1 23 10 [Reporter]SCO3452 (13J12)_PCR/996 [Gene]SCO3452/916 NC_003888 SCE46.09c, probable methyltransferase, len: 359 aa; similar to TR:AAF00618 (EMBL:AF166383) Mus musculus methyltransferase, 336 aa; fasta scores: opt: 868 z-score: 1022.0 E(): 0; 44.7% identity in 320 aa overlap and to SW:GRC2_LACLA (EMBL:L14679) Lactococcus lactis probable menaquinone biosynthesis methyltransferase (EC 2.1.1.-), 252 aa; fasta scores: opt: 221 z-score: 265.2 E(): 2e-07; 38.7% identity in 111 aa overlap. Contains Pfam match to entry PF01209 Ubie_methyltran, ubiE/COQ5 methyltransferase family 2 2 17 20 [Reporter]SCO6813 (3J15)_PCR/2891 [Gene]SCO6813/2634 NC_003888 SC1A2.22, possible ArsR-family transcriptional regulator, len: 129 aa. Highly similar to several including: Streptomyces coelicolor TR:Q9X8X8 (EMBL:AL078610) putative transcriptional regulator, SCH35.28C (122 aa), fasta scores opt: 271 z-score: 365.4 E(): 6e-13 50.0% identity in 92 aa overlap and Mycobacterium tuberculosis TR:P71941 (EMBL:Z80225) putative transcriptional regulator (126 aa), fasta scores opt: 214 z-score: 291.6 E(): 7.7e-09 59.4% identity in 64 aa overlap. Also similar to the adjoining CDS SC1A2.21 (119 aa), fasta scores opt: 292 z-score: 353.0 E(): 3.1e-14 51.1% identity in 94 aa overlap. Contains a Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family. 2 1 23 9 [Reporter]SCO0796 (14F12)_PCR/995 [Gene]SCO0796/915 NC_003888 SCF43.07, possible integral membrane protein, len: 419 aa. Weakly similar to several proteins of undefined function e.g. Streptomyces lividans TR:P72466(EMBL:Z70724) a substrate hyphae associated protein, Orf3 (329 aa), fasta scores opt: opt: 221 z-score: 250.2 E(): 1.5e-06 27.0% identity in 318 aa overlap. Also similar to the upstream CDS SCF43.02, putative integral membrane protein (379 aa), fasta scores opt: 276 z-score: 252.1 E(): 1.2e-08 28.1% identity in 366 aa overlap. Contains possible membrane spanning hydrophobic domains. 2 2 17 18 [Reporter]SCO2384 (5B15)_PCR/2889 [Gene]SCO2384/2633 NC_003888 SC4A7.12, unknown, len: 139 aa 2 1 23 8 [Reporter]SCO3912 (15B12)_PCR/994 [Gene]SCO3912/914 NC_003888 SCH24.34, possible beta-lactamase, len: 461 aa; similar to many e.g. TR:P71420 (EMBL:X92508) Klebsiella pneumoniae ES-beta-lactamase precursor (382 aa), fasta scores; opt: 234 z-score: 262.3 E(): 2.7e-07, 25.7% identity in 378 aa overlap. Weakly similar to TR:O88012 (EMBL:AL031107) S.coelicolor hypothetical protein SC5A7.06c (409 aa) (29.3% identity in 376 aa overlap) 2 2 17 17 [Reporter]SCO6531 (6N11)_PCR/2888 [Gene]SCO6531/2632 NC_003888 SC5C7.16, probable ATP/GTP binding protein, len: 141 aa; highly similar to OSMC_ECOLI osmotically inducible protein C (143 aa), fasta scores; opt: 330 z-score: 398.2 E(): 6.2e-15, 44.5% identity in 137 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 23 7 [Reporter]SCO0967 (16N8)_PCR/993 [Gene]SCO0967/913 NC_003888 SCM11.22c, possible reductase, len: 207 aa; similar to SW:F4RE_METJA (EMBL:U67591) Methanococcus jannaschii putative F420-dependent NADP reductase (EC 1.-.-.-) MJ1501, 223 aa; fasta scores: opt: 209 z-score: 243.7 E(): 3.4e-06; 27.9% identity in 197 aa overlap and to SCM11.25c, 207 aa; fasta scores: opt: 761 z-score: 757.9 E(): 0; 60.3% identity in 204 aa overlap 2 2 17 16 [Reporter]SCO5665 (7J11)_PCR/2887 [Gene]SCO5665/2631 NC_003888 SC6A9.02, unknown, len: 213 aa; some similarity to several hypothetical proteins from Caenorhabditis elegans e.g. TR:G3168942 (EMBL:AF068713) T24A6.7 (206 aa), fasta scores; opt: 256 z-score: 328.5 E(): 4.7e-11, 31.2% identity in 154 aa overlap 2 1 23 6 [Reporter]SCO3400 (17J8)_PCR/992 [Gene]SCO3400/912 NC_003888 SCE9.07, folB, probable dihydroneopterin aldolase, len: 119 aa; similar to many e.g. the N-terminal, dihydroneopterin aldolase domain of SW:SULD_STRPN (EMBL:U16156), sulD, Streptococcus pneumoniae bifunctional dihydroneopterin aldolase/hydroxymethyldihydropterin pyrophosphokinase (270 aa), fasta scores; opt: 240 z-score: 310.8 E(): 5.5e-10, 31.9% identity in 119 aa overlap. Putative start codon suggested by GC frame plot and the presence of a potential RBS 2 2 17 15 [Reporter]SCO5300 (8F11)_PCR/2886 [Gene]SCO5300/2630 NC_003888 SC6G9.33, hypothetical protein, len: 168 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Alternative start codon present at codon 6. Similar to TR:Q9A5F4 (EMBL:AE005918) Caulobacter crescentus hypothetical protein CC2496, 185 aa; fasta scores: opt: 573 Z-score: 686.8 E(): 1.3e-30; 52.532% identity in 158 aa overlap 2 1 23 5 [Reporter]SCO0877 (18F8)_PCR/991 [Gene]SCO0877/911 NC_003888 SCM1.10, possible transcriptional regulatory protein, len: 888 aa. Highly similar to many e.g. Streptomyces hygroscopicus TR:Q54307 (EMBL:X86780) regulator of cholesterol oxidase (872 aa), fasta scores opt: 259 z-score: 271.6 E(): 8.8e-08 29.3% identity in 916 aa overlap and Streptomyces coelicolor TR:CAB52359 (EMBL:AL109747) probable transcriptional regulator SCJ21.13 (919 aa), fasta scores opt: 780 z-score: 819.1 E():0 37.5% identity in 918 aa overlap. Contains a Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). Contains possible membrane spanning hydrophobic domains. Contains a putative helix-turn-helix motif situated between residues 836..857 (+4.18 SD) 2 1 23 4 [Reporter]SCO2234 (19B8)_PCR/990 [Gene]SCO2234/910 NC_003888 SC10B7.29c, glnE, glutamate-ammonia-ligase adenylyltransferase, len: >749 aa; identical to previously sequenced TR:O86858 (EMBL:Y17736) Streptomyces coelicolor glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) GlnE (fragment), 748 aa and similar to SW:GLNE_ECOL (EMBL:Z21844) Escherichia coli glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) GlnE, 946 aa; fasta scores: opt: 442 z-score: 496.8 E(): 2.7e-20; 28.8% identity in 751 aa overlap,SCBAC17D6.01c, glnE, glutamate-ammonia-ligase adenylyltransferase (fragment), len: >285 aa; identical to N-terminal region of previously sequenced TR:O86858 (EMBL:Y17736) Streptomyces coelicolor glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) (glutamine-synthetase adenylyltransferase) (fragment), GlnE, 784 aa 3 3 11 16 [Reporter]SCO7275 (7C6)_PCR/5275 [Gene]SCO7275/4809 NC_003888 SC5H1.17, hypothetical protein, len: 138 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains region similar to adjacent CDS SC5H1.16, hypothetical protein (194 aa) (34.6% identity in 107 aa overlap) 3 3 11 14 [Reporter]SCO2650 (9K2)_PCR/5273 [Gene]SCO2650/4808 NC_003888 SC8E4A.20c, putative secreted protein, len: 103 aa. Contains possible N-terminal region signal peptide sequence 3 3 11 13 [Reporter]SCO6488 (10G2)_PCR/5272 [Gene]SCO6488/4807 NC_003888 SC9C7.24, possible acyl-peptide hydrolase, len: 651aa; similar to many acyl-peptide hydrolases and hypothetical proteins egs. TR:Q55413 (EMBL:D64003) hypothetical protein from Synechocystis sp. (strain PCC 6803) (637 aa) fasta scores; opt: 1306, z-score: 1326.5, E(): 0, (39.6% identity in 647 aa overlap) and TR:P70479 (EMBL:X14915) acyl-peptide hydrolase from Rattus norvegicus (rat) (729 aa) fasta scores; opt: 258, z-score: 199.2, E(): 0.00083, (28.2% identity in 234 aa overlap). 3 3 11 12 [Reporter]SCO2712 (11C2)_PCR/5271 [Gene]SCO2712/4806 NC_003888 SCC61A.33, possible membrane protein, len: 239aa; weakly similar to TR:Q9X4U6 (EMBL:AF118389) from cps locus of Streptococcus suis serotype 2 (229 aa) fasta scores; opt: 161, z-score: 172.0, E(): 0.04, 28.4% identity in 176 aa overlap. Contains possible membrane-spanning hydrophobic regions. 3 3 11 11 [Reporter]SCO4427 (11O22)_PCR/5270 [Gene]SCO4427/4805 NC_003888 SCD6.05c, hypothetical protein, len: 139 aa; similar to TR:P95144 (EMBL:Z83859) Mycobacterium tuberculosis hypothetical 14.7 kD protein MTCY359.02, 129 aa; fasta scores: opt: 462 z-score: 578.8 E(): 8.8e-25; 58.2% identity in 134 aa overlap 3 3 11 10 [Reporter]SCO3537 (12K22)_PCR/5269 [Gene]SCO3537/4804 NC_003888 SCE2.18c, possible DNA-binding protein, len: 390 aa; similar to TR:O53463 (EMBL:AL021899) putative regulator from Mycobacterium tuberculosis (346 aa) fasta scores; opt: 208, z-score: 251.9, E(): 1e-06, (32.8% identity in 302 aa overlap). Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix. 3 3 11 9 [Reporter]SCO1056 (13G22)_PCR/5268 [Gene]SCO1056/4803 NC_003888 SCG22.02, possible sugar transport sugar binding protein, len: 419 aa; similar to TR:CAB90879 (EMBL:AL355752) Streptomyces coelicolor putative maltose-binding protein MalE, 423 aa; fasta scores: opt: 433 z-score: 507.0 E(): 9.2e-21; 28.7% identity in 380 aa overlap and to SW:MALX_STRPN (EMBL:L08611) Streptococcus pneumoniae maltose/maltodextrin-binding protein precursor MalX, 423 aa; fasta scores: opt: 255 z-score: 299.6 E(): 3.3e-09; 23.9% identity in 372 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also a possible N-terminal signal peptide sequence 3 3 11 8 [Reporter]SCO0044 (14C22)_PCR/5267 [Gene]SCO0044/4802 NC_003888 SCJ4.25c, probable oxidoreductase, len: 222 aa; similar to many e.g. SW:NOX_THETH NADH dehydrogenase from Thermus thermophilus (205 aa) fasta scores; opt: 169, z-score: 215.5, E(): 0.00012, (27.7% identity in 220 aa overlap). Contains Pfam match to entry PF00881 Nitroreductase, Nitroreductase family 2 2 17 14 [Reporter]SCO5511 (9B11)_PCR/2885 [Gene]SCO5511/2629 NC_003888 SC8D9.23, possible large membrane associated phophodiesterase , len: 1108aa; the first 400aa of this protein appear to contain several potential membrane spanning regions. The remainder shows similarity to several eg. TR:O87378 (EMBL:AF052519) C-DI-GMP phophodiesterase A from Acetobacter xylinum (740 aa) fasta scores; opt: 683, z-score: 565.4, E(): 3.4e-24, (27.3% identity in 645 aa overlap). Also similar to a nearby gene on the same cosmid (SC8D9.07) fasta scores; opt: 736, z-score: 499.5, E(): 5.6e-23, (32.4% identity in 728 aa overlap). Contains Pfam match to entries PF00989 PAS, PAS domain, score 34.90, E-value 1.8e-06, PF00990 DUF9, Domain of unknown function, score 167.40, E-value 2.3e-46 and PF00563 DUF2, Domain of unknown function 2, score 152.30, E-value 8.3e-42. 3 3 11 7 [Reporter]SCO3154 (15O18)_PCR/5266 [Gene]SCO3154/4801 NC_003888 SCE87.05, possible integral membrane protein, len: 591 aa. Similar to several of undefined function e.g. Mycobacterium tuberculosis TR: O05586 (EMBL: Z94752) hypothetical 55.5 KD protein (503 aa), fasta scores opt: 666 z-score: 775.7 E(): 0 39.0% identity in 544 aa overlap and Aeropyrum pernix TR:BAA79111 (EMBL:AP000058) 418aa long hypothetical protein (418 aa), fasta scores opt: 201 z-score: 235.2 E(): 9.9e-06 30.4% identity in 299 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 2 2 17 13 [Reporter]SCO2511 (10N7)_PCR/2884 [Gene]SCO2511/2628 NC_003888 SCC121.14c, unknown, len: 73 aa 3 3 11 6 [Reporter]SCO0783 (16K18)_PCR/5265 [Gene]SCO0783/4800 NC_003888 3SCF60.15, tetM, tetracycline resistance protein, len: 635 aa; identical to SW:TETM_STRLI (EMBL:M74049) Streptomyces lividans tetracycline resistance protein TetM, 639 aa. Contains Pfam match to entry PF00009 GTP_EFTU, Elongation factor Tu family and PF00679 EFG_C, Elongation factor G C-terminus and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 23 3 [Reporter]SCO6099 (20N4)_PCR/989 [Gene]SCO6099/909 NC_003888 SCBAC1A6.23c, cysC, adenylylsulfate kinase, len: 178 aa; highly similar to C-terminal region of SW:MET3_EMENI (EMBL:X82541) Emericella nidulans sulfate adenylyltransferase (EC 2.7.7.4) Sc, 574 aa; fasta scores: opt: 481 z-score: 561.7 E(): 1.1e-23; 46.0% identity in 174 aa overlap and to N-terminal region of SW:PPS1_HUMAN (EMBL:Y10387) Homo sapiens bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 1 [includes: sulfate adenylyltransferase (EC 2.7.7.4); adenylylsulfate kinase (EC 2.7.1.25)] PapSS1 where the kinase activity is situated, 624 aa; fasta scores: opt: 476 z-score: 555.3 E(): 2.5e-23; 44.3% identity in 174 aa overlap. Contains Pfam match to entry PF01583 APS_kinase, Adenylylsulfate kinase and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 2 17 12 [Reporter]SCO4386 (11J7)_PCR/2883 [Gene]SCO4386/2627 NC_003888 SCD10.18, unknown, len: 203 aa 2 1 22 22 [Reporter]SCO1101 (1F24)_PCR/987 [Gene]SCO1101/908 NC_003888 2SCG4.17, possible integral membrane protein, len: 311 aa; similar to TR:Q9XAS1 (EMBL:AL078618) Streptomyces coelicolor putative integral membrane protein SC10A7.04, 312 aa; fasta scores: opt: 1123 z-score: 1310.0 E(): 0; 56.4% identity in 305 aa overlap. Contains possible hydrophobic membrane spanning regions 2 2 17 11 [Reporter]SCO4460 (12F7)_PCR/2882 [Gene]SCO4460/2626 NC_003888 SCD65.03, hypothetical protein, len: 156 aa; similar to the C-terminal region of the SCP1 plasmid borne TR:Q9AD28 (EMBL:AL590463) Streptomyces coelicolor hypothetical protein SCP1.122, 246 aa; fasta scores: opt: 444 Z-score: 518.8 E(): 2.9e-21; 55.906% identity in 127 aa overlap. Contains match to Prosite entry PS00038 Myc-type, 'helix-loop-helix' dimerization domain signature 2 1 22 21 [Reporter]SCO3668 (2B24)_PCR/986 [Gene]SCO3668/907 NC_003888 SCH44.08c, hspR, putative heat shock protein, len: 151 aa; identical to previously sequenced SW:HSPR_STRCO (EMBL:L46700) Streptomyces coelicolor putative heat shock protein HspR, 151 aa. Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and match to Prosite entry PS00552 Bacterial regulatory proteins, merR family signature. Contains also possible colied-coil region at aprox. residues 78..106 and possible helix-turn-helix motif at residues 18..39 (+3.49 SD) 2 2 17 10 [Reporter]SCO3079 (13B7)_PCR/2881 [Gene]SCO3079/2625 NC_003888 SCE25.20, probable thiolase, len: 406 aa; similar to SW:CATF_ACICA (EMBL:AF009224) Acinetobacter calcoaceticus beta-ketoadipyl CoA thiolase (EC 2.3.1.-) CatF, 401 aa; fasta scores: opt: 957 z-score: 1066.1 E(): 0; 40.0% identity in 415 aa overlap and to TR:CAB45575 (EMBL:AL079355) Streptomyces coelicolor PcaF, beta-ketoadipyl CoA thiolase SC4C6.11c, 400 aa; fasta scores: opt: 895 z-score: 916.7 E(): 0; 38.9% identity in 409 aa overlap. Contains Pfam match to entry PF00108 thiolase, Thiolase and match to Prosite entry PS00737 Thiolases signature 2 2 1 22 20 [Reporter]SCO1387 (3N20)_PCR/985 [Gene]SCO1387/906 NC_003888 SC1A8A.07c, possible membrane associated protein, len: 110 aa; similar to SW:Q50610 (Y0DK_MYCTU) hypothetical protein from Mycobacterium tuberculosis (307 aa) fasta scores; opt: 499, z-score: 537.1, E(): 1.7e-22, 38.3% identity in 282 aa overlap. Contains possible membrane-spanning hydrophobic region 60-75 aa and potential coiled-coil region 100-122 aa 2 2 17 9 [Reporter]SCO0453 (14N3)_PCR/2880 [Gene]SCO0453/2624 NC_003888 SCF51A.31, probable secreted solute-binding lipoprotein, len: 429 aa. Weakly similar to many putative solute-binding proteins e.g. Bacillus subtilis SW:YURO_BACSU (EMBL; Z99120) hypothetical ABC transporter extracellular binding protein YurO (422 aa), fasta scores opt: 235 z-score: 270.1 E(): 1.1e-07 19.9% identity in 422 aa overlap. Contains a Pfam match to entry PF01547 SBP_bacterial_1, an appropriately positioned Prosite hit to PS00013, a prokaryotic membrane lipoprotein lipid attachment site and also contains a possible N-terminal signal sequence. 2 1 22 19 [Reporter]SCO7626 (4J20)_PCR/984 [Gene]SCO7626/905 NC_003888 SC2H2.24c, probable monooxygenase, len: 492 aa; similar to SW:MHPA_ECOLI (EMBL:D86239) Escherichia coli 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-) MhpA, 554 aa; fasta scores: opt: 521 z-score: 602.2 E(): 5.2e-26; 32.6% identity in 356 aa overlap. Contains Pfam matches to entries PF01494 FAD_binding_3, FAD binding domain and PF01360 Monooxygenase, Monooxygenase 2 2 17 8 [Reporter]SCO3834 (15J3)_PCR/2879 [Gene]SCO3834/2623 NC_003888 SCH69.04c, probable 3-Hydroxyacyl-CoA dehydrogenase, len: 504aa; similar to many eg. TR:O84980 (EMBL:AF029714) PhaC, 3-Hydroxyacyl-CoA dehydrogenase from Pseudomonas putida (505 aa) fasta scores; opt: 1512, z-score: 1634.7, E(): 0, (50.0% identity in 498 aa overlap). Contains Pfam match to entry PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase. 2 1 22 18 [Reporter]SCO7112 (5F20)_PCR/983 [Gene]SCO7112/904 NC_003888 SC4B10.13c, probable ECF-family RNA polymerase sigma factor, len: 298 aa; similar to TR:Q9S1V5 (EMBL:AL109950) Streptomyces coelicolor putative sigma factor SCJ24.18c, 250 aa; fasta scores: opt: 1127 z-score: 1362.0 E(): 0; 72.8% identity in 243 aa overlap and to Streptomyces coelicolor SC4B10.06c, 301 aa; fasta scores: opt: 1112 z-score: 1158.1 E(): 0; 57.3% identity in 288 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 2 2 17 7 [Reporter]SCO0942 (16F3)_PCR/2878 [Gene]SCO0942/2622 NC_003888 SCM10.30, possible RNA polymerase sigma factor, len: 184 aa; similar to various sigma factors, e.g. TR:O53798 (EMBL:AL021958) Mycobacterium tuberculosis, and Mycobacterium bovis RNA polymerase sigma factor SigJ, 177 aa; fasta scores: opt: 364 z-score: 454.2 E(): 6.6e-18; 42.0% identity in 174 aa overlap, to TR:O86856 (EMBL:AJ007313) Streptomyces coelicolor sigma factor SigT, 236 aa; fasta scores: opt: 235 z-score: 295.6 E(): 4.5e-09; 35.4% identity in 161 aa overlap, to SW:RPSC_MYCTU (EMBL:Z73966) Mycobacterium tuberculosis probable RNA polymerase sigma-C factor SigC, 185 aa; fasta scores: opt: 216 z-score: 274.0 E(): 7.1e-08; 33.5% identity in 161 aa overlap and to S. coelicolor StF56.16, 194 aa; fasta scores: opt: 200 z-score: 206.6 E(): 4.2e-06; 31.9% identity in 144 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 2 1 22 17 [Reporter]SCO7568 (6B20)_PCR/982 [Gene]SCO7568/903 NC_003888 SC5F1.22c, possible regulatory protein, len: 319 aa; similar to TR:O50502 (EMBL:AL009199) Streptomyces coelicolor probable transcriptional repressor protein SC7B7.05, 403 aa; fasta scores: opt: 377 z-score: 404.3 E(): 5.5e-15; 29.3% identity in 290 aa overlap. Contains Pfam match to entry PF00480 ROK, ROK family 2 2 17 6 [Reporter]SCO3811 (17B3)_PCR/2877 [Gene]SCO3811/2621 NC_003888 SCGD3.12, dacA, probable D-alanyl-D-alanine carboxypeptidase, len: 314 aa; highly similar to SW:DACX_STRSK (EMBL:X59965) Streptomyces sp. (strain K15) D-alanyl-D-alanine carboxypeptidase (291 aa), fasta scores; opt: 1322 z-score: 1435.8 E(): 0, 73.8% identity in 302 aa overlap. Also similar to many other penicillin-binding proteins. Contains Pfam match to entry PF00768 Peptidase_S11, D-alanyl-D-alanine carboxypeptidase and PS00017 ATP/GTP-binding site motif A (P-loop). Contains possible hydrophobic membrane spanning region 2 1 22 16 [Reporter]SCO2115 (7N16)_PCR/981 [Gene]SCO2115/902 NC_003888 SC6E10.09c, aroH, probable 2-dehydro-3-deoxyphosphoheptonate aldolase, len: 450 aa; highly similar to many, especially those from plants e.g. SW:AROF_ARATH (EMBL:M74819), Dhs1, Arabidopsis thaliana phospho-2-dehydro-3-deoxyheptonate aldolase 1 precursor (525 aa), fasta scores; opt: 1633 z-score: 1839.4 E(): 0, 53.8% identity in 437 aa overlap. The N-terminus is similar (but not identical) to the SW:AROF_STRCO, AroH, Streptomyces coelicolor 2-dehydro-3-deoxyphosphoheptonate aldolase N-terminal sequence determined from purified protein (TVNAKTSPSA GNTDDPLQAP S). Similar to TR:O52308 (EMBL:AF035756) Streptomyces sp. 2-dehydro-3-deoxyphosphoheptonate aldolase (fragment) (448 aa) (90.4% identity in 448 aa overlap). Also similar to TR:Q9Z4X1 (EMBL:AL035654), Aro, S.coelicolor probable 2-dehydro-3-deoxyheptonate aldolase (484 aa) (53.6% identity in 440 aa overlap). An alternative start codon is present at codon 3. Contains Pfam match to entry PF01474 DAHP_synth_2, Class-II DAHP synthetase family 2 2 17 5 [Reporter]SCO0653 (17N23)_PCR/2876 [Gene]SCO0653/2620 NC_003888 SCF91.13, unknown, len: 183 aa. Similar to two proteins of unknown function from Streptomyces coelicolor TR:CAB44390(EMBL:AL078610) SCH35.37C (199 aa), fasta scores opt: 378 z-score: 365.6 E(): 5.5e-13 38.3% identity in 196 aa overlap and TR: Q9ZC10(EMBL:AL033505) SC1E6.12 (239 aa), fasta scores opt: 327 z-score: 317.7 E(): 2.6e-10 35.1% identity in 228 aa overlap. 2 1 22 15 [Reporter]SCO2007 (8J16)_PCR/980 [Gene]SCO2007/901 NC_003888 SC7H2.21, unknown, len: 198aa; 2 1 22 14 [Reporter]SCO7544 (9F16)_PCR/979 [Gene]SCO7544/900 NC_003888 SC8G12.20, possible ABC-transport protein, membrane component, len: 267 aa. Similar to many other transporters e.g. Escherichia coli SW:TAUC_ECOLI(EMBL:D85613) taurine transport permease protein, TauC (275 aa), fasta scores opt: 547 z-score: 643.1 E(): 2.3e-28 37.5% identity in 248 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. 2 2 17 4 [Reporter]SCP1.06c (18J23)_PCR/2875 [Gene]SCP1.06c/2619 NC_003888 SCP1.06c, unknown, len: 74aa; 2 2 17 3 [Reporter]SCO1865 (19F23)_PCR/2874 [Gene]SCO1865/2618 NC_003888 SCI39.12, probable aminotransferase, len: 423 aa; similar to SW:ECTB_MARHA (EMBL:U66614) Marinococcus halophilus diaminobutyrate--pyruvate aminotransferase (EC 2.6.1.46) EctB, 427 aa; fasta scores: opt: 1512 Z-score: 1688.4 bits: 321.4 E(): 1.9e-86; 53.333% identity in 420 aa overlap. Contains 2x Pfam matches to entry PF00202 aminotran_3, Aminotransferase class-III pyridoxal-phosphate and and match to Prosite entry PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site 2 2 17 2 [Reporter]SCO5098 (20N19)_PCR/2873 [Gene]SCO5098/2617 NC_003888 SCBAC28G1.24, possible integral membrane protein, len: 154 aa; identical in its C-terminal domain to TR:Q9X9U7 (EMBL:Y18817) Streptomyces coelicolor A3(2) hypothetical 11.9 kDa protein ORF11, 113 aa. Contains possible hydrophobic membrane spanning regions and 10x degenerate repeat at N-terminal region: M(P/V). Alternative start codon in previously sequence EMBL entrance after the repeat region at residue 42 2 2 16 22 [Reporter]SCO1221 (1N15)_PCR/2871 [Gene]SCO1221/2616 NC_003888 2SCG58.21, possible AsnC-family regulatory protein, len: 167 aa; similar to SW:PUTR_RHOCA (EMBL:X78346) Rhodobacter capsulatus proline dehydrogenase transcriptional activator PutR, 154 aa; fasta scores: opt: 248 z-score: 311.6 E(): 6.9e-10; 31.3% identity in 147 aa overlap. Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family 2 2 16 21 [Reporter]SCO5553 (2J15)_PCR/2870 [Gene]SCO5553/2615 NC_003888 SC1C2.34, leuC, 3-isopropylmalate dehydratase large subunit, len: 476 aa; highly similar to many e.g. LEU2_ECOLI 3-isopropylmalate dehydratase large subunit (465 aa), fasta scores; opt: 1943 z-score: 2277.9 E(): 0, 63.3% identity in 458 aa overlap. Contains PS00450 and PS01244 Aconitase family signatures 1 and 2 and Pfam match to entry PF00330 aconitase, Aconitase family (aconitate hydratase), score 760.00, E-value 6.9e-232 2 2 16 20 [Reporter]SCO7793 (3F15)_PCR/2869 [Gene]SCO7793/2614 NC_003888 SC10B8A.03c, unknown, doubtful CDS, len: 100 aa 2 2 16 19 [Reporter]SCO4819 (4B15)_PCR/2868 [Gene]SCO4819/2613 NC_003888 SC2A6.04c, possible secreted protein, len: 156 aa. Contains possible N-terminal region signal peptide sequence 2 2 16 18 [Reporter]SCO2103 (5N11)_PCR/2867 [Gene]SCO2103/2612 NC_003888 SC4A10.36c, metF, 5,10-methylenetetrahydrofolate reductase, len: 307 aa; identical to SW:METF_STRLI (EMBL:AJ001630), MetF, Streptomyces lividans 5,10-methylenetetrahydrofolate reductase (307 aa), apart from substitution of Arg41 for Lys41 in S.coelicolor, fasta scores; opt: 2015 z-score: 2364.3 E(): 0, 99.7% identity in 307 aa overlap 2 2 16 17 [Reporter]SCO6527 (6J11)_PCR/2866 [Gene]SCO6527/2611 NC_003888 SC5C7.12, probable membrane protein, len: 717 aa; contains large hydrophobic domain at N-terminus 2 2 16 16 [Reporter]SCO5700 (7F11)_PCR/2865 [Gene]SCO5700/2610 NC_003888 SC5H4.24c, probable kinase, len: 299 aa; similar to SW:STRA_STRGA (EMBL:X05648) Streptomyces glaucescens streptomycin 6-kinase (EC 2.7.1.72) Sph, 307 aa; fasta scores: opt: 446 z-score: 504.5 E(): 1.2e-20; 34.7% identity in 271 aa overlap. Contains possible helix-turn-helix motif at residues 249..270 (+2.59 SD) 2 2 16 15 [Reporter]SCO5304 (8B11)_PCR/2864 [Gene]SCO5304/2609 NC_003888 SC6G9.29, possible sensor-like histidine kinase, len: 414 aa; similar to many sensor histidine kinases from two-component systems e.g. (EMBL:AJ13121), CseC, S.coelicolor sensory histidine kinase of two-component system regulating sigE transcription (507 aa), fasta scores; opt: 966 z-score: 1051.7 E(): 0, 44.3% identity in 429 aa overlap. This protein does not have an obvious response regulator companion. Similar to part of another lone sensor-like histidine kinase TR:O05890 (EMBL:Z95121), Mycobacterium tuberculosis sensor-like histidine kinase (567 aa) (33.8% identity in 397 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam matches to entry PF00672 DUF5, Domain found in bacterial signal proteins and to entry PF00512 signal, Signal carboxyl-terminal domain. Contains possible hydrophobic membrane spanning regions 2 2 16 14 [Reporter]SCO6978 (9N7)_PCR/2863 [Gene]SCO6978/2608 NC_003888 SC8F11.04c, possible carbohydrate kinase, len: 320 aa. Similar to many fructokinases (EC 2.7.1.4) including: Klebsiella pneumoniae SW:SCRK_KLEPN (EMBL:X61004) ScrK (307 aa), fasta scores opt: 378 z-score: 438.7 E(): 5.3e-17 32.8% identity in 311 aa overlap. Also similar to Bacillus subtilis SW:IOLC_BACSU(EMBL:D14399) hypothetical protein, IolC, found within the inositol utilisation operon (325 aa), fasta scores opt: 583 z-score: 672.7 E(): 4.9e-30 30.0% identity in 313 aa overlap. Contains a Prosite hit to PS00583 pfkB family of carbohydrate kinases signature 1 and a Pfam match to entry PF00294 pfkB, pfkB family carbohydrate kinase. 2 2 16 13 [Reporter]SCO2506 (10J7)_PCR/2862 [Gene]SCO2506/2607 NC_003888 SCC121.09, probable metal transport ABC transporter, len: 256 aa; similar to TR:Q9XDA6 (EMBL:AF104349) Listeria monocytogenes ABC transporter ZurA, 257aa; fasta scores: opt: 468 z-score: 522.7 E(): 1e-21; 38.0% identity in 237 aa overlap and to SW:FHUC_ECOLI (EMBLX05810:) Escherichia coli ferrichrome transport ATP-binding protein FhuC, 265 aa; fasta scores: opt: 416 z-score: 465.6 E(): 1.5e-18; 34.8% identity in 256 aa overlap. Contains Pfam match to entry PF00005 ABC_tran and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 2 16 12 [Reporter]SCO2540 (11F7)_PCR/2861 [Gene]SCO2540/2606 NC_003888 SCC77.07c, possible carbohydrate kinase, len: 396 aa. Weakly similar to many kinases including: Escherichia coli SW:RBSK_ECOLI(EMBL:M13169) ribokinase (EC 2.7.1.15) (309 aa), fasta scores opt: 283 z-score: 306.1 E(): 1.2e-09 29.3% identity in 297 aa overlap. Contains a Prosite hit to PS00584 pfkB family of carbohydrate kinases signature 2 and a Pfam match to entry PF00294 pfkB. Rich in the amino acid Ala. 2 2 16 11 [Reporter]SCO4812 (12B7)_PCR/2860 [Gene]SCO4812/2605 NC_003888 SCD63A.23c, possible integral membrane protein, len: 265 aa. Contains possible hydrophobic membrane spanning regions 2 2 16 10 [Reporter]SCO3044 (13N3)_PCR/2859 [Gene]SCO3044/2604 NC_003888 SCE34.25, conserved hypothetical protein, len: 507 aa; similar to TR:O68907 (EMBL:AF05830) Streptomyces roseofulvus FrnA, 770 aa; fasta scores: opt: 711 z-score: 770.7 E(): 0; 33.9% identity in 508 aa overlap, to Streptomyces coelicolor SCE34.27 (fagment), >356 aa; fasta scores: opt: 1210 z-score: 1128.7 E(): 0; 51.5% identity in 359 aa overlap, to SCE34.26, 507 aa; fasta scores: opt: 739 z-score: 689.6 E(): 6.7e-33; 33.1% identity in 508 aa overlap, to SCE34.23, 404 aa; fasta scores: opt: 671 z-score: 628.0 E(): 1.8e-29; 33.2% identity in 365 aa overlap and to SCE34.24, 472 aa; fasta scores: opt: 644 z-score: 601.9 E(): 5.1e-28; 34.6% identity in 509 aa overlap. Contains possible hydrophobic membrane spanning region 2 2 16 9 [Reporter]SCO1710 (14J3)_PCR/2858 [Gene]SCO1710/2603 NC_003888 SCI30A.31c, possible integral membrane transport protein, len: 483aa; similar to SW:CITM_BACSU Mg2+/citrate complex secondary transporter from Bacillus subtilis (433 aa) fasta scores; opt: 687, z-score: 644.4, E(): 1.5e-28, (45.7% identity in 484 aa overlap). Contains Gly/Ala-rich region 220-250aa. Contains possible membrane spanning hydrophobic regions. 2 2 16 8 [Reporter]SCO1706 (15F3)_PCR/2857 [Gene]SCO1706/2602 NC_003888 SCI30A.27c, probable aldehyde dehydrogenase, len: 462 aa; similar to many e.g. TR:O33455 (EMBL:U24215) P-cumic aldehyde dehydrogenase from Pseudomonas putida Fl (494 aa) fasta scores; opt: 1109, z-score: 1142.8, E(): 0, (44.6% identity in 471 aa overlap) and TR:O88069 (EMBL:AL031541) putative aldehyde dehydrogenase from Streptomyces coelicolor (483 aa) fasta scores; opt: 1011, z-score: 1042.5, E(): 0, (41.8% identity in 466 aa overlap). Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase and Prosite match to PS00687 Aldehyde dehydrogenases glutamic acid active site. 2 2 16 7 [Reporter]SCO3540 (16B3)_PCR/2856 [Gene]SCO3540/2601 NC_003888 SCH5.02c, slpD, proteinase (putative secreted protein), len: 539 aa; identical to the previously sequenced TR:Q54398 (EMBL:L42758) SlpD, mycelium-associated (lipoprotein) proteinase from Streptomyces lividans 66 (539 aa) fasta scores; opt: 3608, z-score: 3750.3, E(): 0, (100.0% identity in 539 aa overlap). Also similar to two other peptidases from Streptomyces coelicolor SC9C7.15c (EMBL:AL035161) fasta scores; opt: 666, z-score: 603.6, E(): 9.4e-29, (35.7% identity in 502 aa overlap) and SC1B5.03c (EMBL:AL023517) fasta scores; opt: 661, z-score: 598.9, E(): 1.7e-28, (32.6% identity in 562 aa overlap). Contains probable N-terminal signal sequence. Also contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold, score 56.80, E-value 4.7e-13. 2 2 16 6 [Reporter]SCO0828 (16N23)_PCR/2855 [Gene]SCO0828/2600 NC_003888 SCF43A.18, probable alkaline phosphatase, len: 553 aa; similar to SW:PPBD_BACSU (EMBL:U49060), PhoD, Bacillus subtilis alkaline phosphatase D precursor (556 aa), fasta scores; opt: 1512 z-score: 1782.1 E(): 0, 47.8% identity in 485 aa overlap 4 3 13 12 [Reporter]SCO2356 (11L2)_PCR/5819 [Gene]SCO2356/5299 NC_003888 SCC8A.14, possible membrane protein, len: 467aa; contains a possible membrane-spanning hydrophobic region close to the N-terminus. 4 3 13 11 [Reporter]SCO4771 (12H2)_PCR/5818 [Gene]SCO4771/5298 NC_003888 SCD63.03, possible inosine-5'-monophosphate dehydrogenase, len: 374 aa; N-terminal region identical to previously sequenced TR:O86845 (EMBL:AJ010601) Streptomyces coelicolor hypothetical 38.9 kD protein (fragment), 84 aa and whole CDS similar to SW:IMDH_ECOLI (EMBL:X02209) Escherichia coli inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) GuaB, 488 aa; fasta scores: opt: 275 z-score: 311.9 E(): 6.1e-10; 29.0% identity in 376 aa overlap. Contains Pfam matches to entries PF01574 IMPDH_N, IMP dehydrogenase / GMP reductase N terminus PF00478 IMPDH_C, IMP dehydrogenase / GMP reductase C terminus 4 3 13 10 [Reporter]SCO2862 (13D2)_PCR/5817 [Gene]SCO2862/5297 NC_003888 SCE20.36c, unknown, len: 77 aa. 4 3 13 9 [Reporter]SCO3215 (13P22)_PCR/5816 [Gene]SCO3215/5296 NC_003888 SCE8.08c, unknown, len: 338 aa 4 3 13 8 [Reporter]SCO3756 (14L22)_PCR/5815 [Gene]SCO3756/5295 NC_003888 SCH63.03c, possible two component system response regulator, len: 224 aa; similar to TR:Q9X8Q7 (EMBL:AL049754) Streptomyces coelicolor putative two-component system regulator SCH10.18c, 228 aa; fasta scores: opt: 827 z-score: 946.4 E(): 0; 62.1% identity in 219 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature 4 3 13 7 [Reporter]SCO0167 (15H22)_PCR/5814 [Gene]SCO0167/5294 NC_003888 SCJ1.16c, unknown, len: 294 aa, similar to many including: Mycobacterium tuberculosis TR:O06189 (EMBL; Z95387)hypothetical 31.7 KD protein MTCY1A10.09C (297 aa), fasta scores opt: 407 z-score: 484.5 E(): 1.3e-19 32.7% identity in 303 aa overlap and Mycobacterium tuberculosis SW:YW12_MYCTU (EMBL; Z74025) hypothetical 30.9 KD protein MTCY39.12 (295 aa), fasta scores opt: 401 z-score: 477.5 E(): 3.1e-19 34.7% identity in 294 aa overlap. Also similar to S. coelicolor SCJ1.29c (283 aa), fasta scores opt: 806 z-score: 761.9 E(): 0 46.4% identity in 295 aa overlap. Contains a Pfam match to entry PF00582 Usp, Universal stress protein family. 4 3 13 6 [Reporter]SCO1028 (16D22)_PCR/5813 [Gene]SCO1028/5293 NC_003888 SCG20A.08c, possible lyase, len: 374 aa; highly similar to TR:O69652 (EMBL:AL022121) Mycobacterium tuberculosis putative lyase MTV025.032, 346 aa; fasta scores: opt: 1469 z-score: 1762.4 E(): 0; 63.3% identity in 341 aa overlap. Contains Pfam match to entry PF00291 S_T_dehydratase, Pyridoxal-phosphate dependent enzyme 4 3 13 3 [Reporter]SCO4989 (19H18)_PCR/5810 [Gene]SCO4989/5292 NC_003888 2SCK36.12, probable IclR-family transcriptional regulatory protein, len: 254 aa; similar to SW:KDGR_ECOLI (EMBL:AE000277) Escherichia coli transcriptional regulator KdgR, 263 aa; fasta scores: opt: 420 Z-score: 488.7 bits: 98.0 E(): 1.3e-19; 30.992% identity in 242 aa overlap. Contains Pfam match to entry PF01614 IclR, Bacterial transcriptional regulator and possible helix-turn-helix motif at residues 20..41 (+3.12 SD) 4 3 13 2 [Reporter]SCO0974 (20P14)_PCR/5809 [Gene]SCO0974/5291 NC_003888 SCBAC19F3.01c, possible secreted protein, len: >237aa: interesting similarity to TR:O86308 (EMBL:Z96935) a resuscitation-promoting factor from Micrococcus luteus (220 aa) fasta scores; opt: 499, Z-score: 540.6, 41.784% identity (45.408% ungapped) in 213 aa overlap. Similar to many others eg. TR:Q9F2Q2 (EMBL:AL442120) putative secreted protein from Streptomyces coelicolor (244 aa) fasta scores; opt: 461, Z-score: 499.7, 44.033% identity (51.442% ungapped) in 243 aa overlap. Contains Pfam match to entry PF01476 LysM, LysM domain and has a possible N-terminal signal sequence.,SCM11.29c, probable secreted protein (partial CDS), len: >234 aa; similar to TR:Q9X7W8 (EMBL:AL049485) Streptomyces coelicolor putative secreted peptidase SC6A5, 312 aa; fast ascores: opt: 684 z-score: 619.7 E(): 3.9e-27; 55.2% identity in 183 aa overlap and to SW:ALE1_STACP (EMBL:D86328) Staphylococcus capitis glycyl-glycine endopeptidase Ale-1 precursor (EC 3.4.24.75), 362 aa; fasta scores: opt: 313 z-score: 291.5 E(): 7.4e-09; 29.3% identity in 229 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 3 12 22 [Reporter]SCO1260 (1P10)_PCR/5807 [Gene]SCO1260/5290 NC_003888 2SCG18.07, probable two-component system response regulator, len: 220 aa; similar to TR:CAB95289 (EMBL:AL359779) Streptomyces coelicolor putative two-component system response regulator SCG22.16c, 221 aa; fasta scores: opt: 756 z-score: 640.4 E(): 4.5e-30; 57.7% identity in 222 aa overlap and to TR:Q9Z677 (EMBL:AF102271) Streptomyces coelicolor response-regulator, 213 aa; fasta scores: opt: 610 z-score: 693.5 E(): 3.8e-31; 50.7% identity in 215 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Also contains possible helix-turn-helix motif at residues 172..193 (+3.03 SD) 4 3 12 21 [Reporter]SCO3669 (2L10)_PCR/5806 [Gene]SCO3669/5289 NC_003888 SCH44.09c, dnaJ, molecular chaperone, len: 399 aa; identical to previously sequenced SW:DNAJ_STRCO (EMBL:L46700) Streptomyces colicolor DnaJ protein, 399 aa and similar to TR:CAB66232 (EMBL:AL136503) Streptomyces coelicolor SCC77.21c heat shock protein DnaJ2, 378 aa; fasta scores: opt: 713 z-score: 611.9 E(): 1.5e-28; 38.8% identity in 397 aa overlap. Contains Pfam matches to entries PF00226 DnaJ, DnaJ domain, PF00684 DnaJ_CXXCXGXG, DnaJ central domain (4 repeats) and PF01556 DnaJ_C, DnaJ C terminal region and matches to Prosite entries PS00636 Nt-dnaJ domain signature and PS00637 CXXCXGXG dnaJ domain signature 4 3 12 20 [Reporter]SCO1483 (3H10)_PCR/5805 [Gene]SCO1483/5288 NC_003888 SC9C5.07c, pyrA, carbamoylphosphate synthetase large chain, len: 1102 aa; similar to SW:CARB_ECOLI (EMBL:V01500) Escherichia coli carbamoyl-phosphate synthase large chain (EC 6.3.5.5) CarB or PyrA, 1072 aa; fasta scores: opt: 3383 z-score: 3699.6 E(): 0; 55.0% identity in 1102 aa overlap. Contains 3x Pfam matches to entry PF00289 CPSase_L_chain, Carbamoyl-phosphate synthase (CPSase) and matches to Prosite entries 2x PS00866 Carbamoyl-phosphate synthase subdomain signature 1 and 2x PS00867 Carbamoyl-phosphate synthase subdomain signature 4 3 12 19 [Reporter]SCO5550 (4D10)_PCR/5804 [Gene]SCO5550/5287 NC_003888 SC1C2.31c, probable transcriptional regulator, len: 214 aa; N-terminus is similar to the N-terminus of members of the MerR family, e.g. MERR_THIFE mercuric resistance operon regulatory protein (122 aa), fasta scores; opt: 111 z-score: 189.7 E(): 0.0026, 30.3% identity in 66 aa overlap. Contains probable helix-turn-helix motif at aa 2-23 (1799, +5.31 SD) 4 3 12 18 [Reporter]SCO2201 (5P6)_PCR/5803 [Gene]SCO2201/5286 NC_003888 SC3H12.09, conserved hypothetical protein, len: 123 aa; identical to previously sequenced TR:CAB58283 (EMBL:AL121850) Streptomyces coelicolor conserved hypothetical protein SCC78.02, 123 aa and similar to TR:Q9ZBQ5 (EMBL:AL034447) Streptomyces coelicolor hypothetical 17.8 kD protein SC7A1.18c, 141 aa; fasta scores: opt: 317 z-score: 412.3 E(): 1.4e-15; 44.6% identity in 112 aa overlap. Contains Pfam match to entry PF01638 DUF24, Protein of unknown function 4 3 12 17 [Reporter]SCO5823 (6L6)_PCR/5802 [Gene]SCO5823/5285 NC_003888 SC5B8.13, probable integral membrane protein, len: 1 74 aa; contains five possible transmembrane domains 4 3 12 16 [Reporter]SCO7270 (7H6)_PCR/5801 [Gene]SCO7270/5284 NC_003888 SC5H1.22, probable transcriptional regulator, len: 294 aa; similar to many LysR family regulators e.g. SW:BUDR_KLETE (EMBL:Z48600), budR, Klebsiella terrigena bud operon transcriptional regulator (290 aa), fasta scores; opt: 392 z-score: 459.0 E(): 3.1e-18, 33.7% identity in 267 aa overlap. Similar to TR:O86805 (EMBL:AL031317) S.coelicolor probable transcriptional regulator (306 aa) (29.3% identity in 304 aa overlap). Contains probable helix-turn-helix motif at aa 17-38 (Score 1145, +3.09 SD). Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix proteins, lysR family, score 171.10, E-value 1.9e-47 and PS00044 Bacterial regulatory proteins, lysR family signature 4 3 12 15 [Reporter]SCO4751 (8D6)_PCR/5800 [Gene]SCO4751/5283 NC_003888 SC6G4.29, probable acetyltransferase, len: 177 aa; s imilar to e.g. RIMI_ECOLI ribosomal-protein-alanine acetyl transferase (148 aa), fasta scores; opt: 166 z-score: 257.4 E(): 4.5e-07, 33.3% identity in 120 aa overlap. Contains Pf am match to entry PF00583 Acetyltransf, Acetyltransferase ( GNAT) family, score 59.60, E-value 6.7e-14 4 3 12 14 [Reporter]SCO2655 (9P2)_PCR/5799 [Gene]SCO2655/5282 NC_003888 SC8E4A.25c, hypothetical protein, len: 178 aa; similar to various hypothetical proteins, e.g. TR:Q9X7B4 (EMBL:AL049913) Mycobacterium leprae hypothetical 23.9 kD protein MLCB1610.15, 215 aa; fasta scores: opt: 589 z-score: 757.6 E(): 0; 52.8% identity in 161 aa overlap. Contains Pfam match to entry PF01844 HNH, HNH endonuclease 4 3 12 13 [Reporter]SCO6258 (10L2)_PCR/5798 [Gene]SCO6258/5281 NC_003888 SCAH10.23, probable ABC transport system sugar permease, len: 346 aa; similar to TR:O68120 (EMBL:AF010496) Rhodobacter capsulatus ribose transport system permease protein RbsC; fasta scores: opt: 445 z-score: 508.0 E(): 6.4e-21; 30.5% identity in 351 aa overlap and to SW:RBSC_ECOLI (EMBL:L10328) Escherichia coli ribose transport system permease protein RbsC, len: 321 aa; fasta scores: opt: 286 z-score: 329.9 E(): 5.3e-11; 30.1% identity in 329 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 12 12 [Reporter]SCO2257 (11H2)_PCR/5797 [Gene]SCO2257/5280 NC_003888 SCC75A.03, probable ABC transporter, ATP-binding component, len: 365 aa. Highly similar to many ABC transport proteins e.g. Streptomyces peucetius SW:DRRA_STRPE(EMBL:M73758) daunorubicin resistance ATP-binding protein (330 aa), fasta scores opt: 922 z-score: 1009.0 E():0 48.9% identity in 329 aa overlap and Streptomyces antibioticus TR:Q53716(EMBL:L06249) ATP-binding protein OLEC-ORF4 involved in oleandomycin-resistance (325 aa), fasta scores opt: 1883 z-score: 2052.8 E():0 89.7% identity in 329 aa overlap. Also similar to several Streptomyces coelicolor putative ABC transporter ATP-binding components e.g TR:CAB42771(EMBL:AL049841) SCE9.25C (315 aa), fasta scores opt: 1031 z-score: 1127.7 E():0 54.4% identity in 318 aa overlap. Contains Prosite hits to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also contains a Pfam match to entry PF00005 ABC_tran, ABC transporter. 4 3 12 11 [Reporter]SCO4456 (12D2)_PCR/5796 [Gene]SCO4456/5279 NC_003888 SCD6.34, possible membrane-associated oxidoreductase, len: 528 aa; similar to TR:CAB52970 (EMBL:AL109950) Streptomyces coelicolor putative membrane-associated oxidoreductase SCJ4.36c, 503 aa; fasta scores: opt: 657 z-score: 776.7 E(): 0; 38.8% identity in 551 aa overlap. Contains possible C-terminal region hydrophobic membrane spanning domains 4 3 12 9 [Reporter]SCO5021 (13L22)_PCR/5794 [Gene]SCO5021/5278 NC_003888 SCK15.23, possible lipoprotein, len: 221 aa; similar to Streptomyces coelicolor SCK15.24, 220 aa; fasta scores: opt: 736 z-score: 652.9 E(): 8.3e-31; 54.3% identity in 221 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 3 12 8 [Reporter]SCO0161 (14H22)_PCR/5793 [Gene]SCO0161/5277 NC_003888 SCJ1.10c, doubtful CDS, len:124 aa. 4 3 12 7 [Reporter]SCO3421 (15D22)_PCR/5792 [Gene]SCO3421/5276 NC_003888 SCE9.28c, hypothetical protein, len: 274 aa; similar others from S.coelicolor e.g. TR:O86719 (EMBL:AL031515), SC5C7.22C, hypothetical protein (287 aa), fasta scores; opt: 727 z-score: 871.9 E(): 0, 44.7% identity in 273 aa overlap 4 3 12 6 [Reporter]SCO5143 (16P18)_PCR/5791 [Gene]SCO5143/5275 NC_003888 SCP8.06, tag, DNA-3-methyladenine glycosylase I, len: 194 aa; highly similar to SW:3MG1_ECOLI (EMBL:J02606) Escherichia coli DNA-3-methyladenine glycosylase I (EC 3.2.2.20) Tag, 187 aa; fasta scores: opt: 529 z-score: 636.5 E(): 5.5e-28; 45.2% identity in 177 aa overlap 4 3 12 5 [Reporter]SCO3890 (17L18)_PCR/5790 [Gene]SCO3890/5274 NC_003888 SCH24.12c, trxB, thioredoxin reductase (NADPH), len: 322 aa; previously sequenced as SW:TRXB_STRCO (EMBL:AJ007313), TrxB. Contains PS00573 Pyridine nucleotide-disulphide oxidoreductases class-II active site 4 3 12 4 [Reporter]SCO1705 (18H18)_PCR/5789 [Gene]SCO1705/5273 NC_003888 SCI30A.26c, probable alcohol dehydrogenase (zinc-binding), len: 365 aa; similar to many e.g. SW:ADH3_ECOLI alcohol dehydrogenase class III from Escherichia coli (369 aa) fasta scores; opt: 819, z-score: 864.3, E(): 0, (38.6% identity in 368 aa overlap). Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and Prosite matche to PS00059 Zinc-containing alcohol dehydrogenases signature. 4 3 12 3 [Reporter]SCO1307 (19D18)_PCR/5788 [Gene]SCO1307/5272 NC_003888 SCBAC36F5.18c, unknown, len: 468 aa 3 2 18 7 [Reporter]SCO1777 (16K3)_PCR/3404 [Gene]SCO1777/3099 NC_003888 SCI51.17, conserved hypothetical protein, len: 612 aa; unknown function, similar to TR:P71741 (EMBL:Z81368) Mycobacterium tuberculosis hypothetical protein (642 aa), fasta scores; opt: 1146 z-score: 1320.1 E(): 0, 40.6% identity in 623 aa overlap and SW:YAY3_SCHPO (EMBL:Z69380) Schizosaccharomyces pombe hypothetical protein (649 aa) (33.5% identity in 641 aa overlap). Also similar to SCE36.11C (EMBL:AL049763) S.coelicolor conserved hypothetical protein (fragment) (612 aa) (42.6% identity in 615 aa overlap) 4 3 12 2 [Reporter]SCO2814 (20L14)_PCR/5787 [Gene]SCO2814/5271 NC_003888 SCBAC17F8.05c, possible methyltransferase, len: 285 aa: similar to many some of which reside within antibiotic biosynthetic clusters e.g. TR:Q54818 (EMBL:L35560) doxorubicin polyketide biosynthesis gene from Streptomyces peucetius (286 aa) fasta scores; opt: 258, Z-score: 287.1, 38.136% identity (39.130% ungapped) in 118 aa overlap, TR:Q9S0N6 (EMBL:AB032524) C5-O-methyltransferase AveD from the avermectin biosynthetic cluster of Streptomyces avermitilis (283 aa) fasta scores; opt: 249, Z-score: 277.5, 30.508% identity (34.123% ungapped) in 236 aa overlap and TR:AAK64748 (EMBL:AE007203) hypothetical protein from Rhizobium meliloti (261 aa) fasta scores; opt: 581, Z-score: 635.1, 41.406% identity (42.570% ungapped) in 256 aa overlap. 3 2 18 6 [Reporter]SCO0530 (17G3)_PCR/3403 [Gene]SCO0530/3098 NC_003888 SCF11.10c, possible transcriptional regulator, len: 281 aa; similar to SW:FARR_ECOLI (EMBL:X15790) Escherichia coli fatty acyl responsive regulator (P30 protein), 240 aa; fasta scores: opt: 286 z-score: 325.5 E(): 9.2e-11; 27.7% identity in 224 aa overlap. Contains Pfam match to entry PF00392 gntR, bacterial regulatory proteins, gntR family and Prosite match to entry PS00043 bacterial regulatory proteins, gntR family signature. Contains a possible helix-turn-helix motif between residues 36..57, score 1211 (+3.31 SD) 4 3 11 22 [Reporter]SCO1256 (1L10)_PCR/5785 [Gene]SCO1256/5270 NC_003888 2SCG18.03c, unknown, len: 146 aa 3 2 18 4 [Reporter]SCO1871 (18O23)_PCR/3401 [Gene]SCO1871/3097 NC_003888 SCI39.18c, probable aldehyde dehydrogenase, len: 509 aa; similar to TR:Q56694 (EMBL:U39638) Vibrio harveyi fatty aldehyde dehydrogenase AldH, 510 aa; fasta scores: opt: 1209 Z-score: 1264.1 bits: 243.5 E(): 8e-63; 42.300% identity in 487 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family 3 2 18 3 [Reporter]SCO4981 (19K23)_PCR/3400 [Gene]SCO4981/3096 NC_003888 2SCK36.04c, possible bifunctional protein, len: 466 aa; N-terminal region presents low similarity to N-terminal region of TR:O14362 (EMBL:Z97992) Schizosaccharomyces pombe putative glucan synthase-1 (EC 2.4.1.34) SPBC30D10.17c, 504 aa; fasta scores: opt: 176 Z-score: 188.3 bits: 44.3 E(): 0.0071; 24.931% identity in 361 aa overlap and C-terminal region to C-terminal region of TR:AAK22951 (EMBL:AE005774) Caulobacter crescentus oxidoreductase, GFO/IDH/MOCA family CC0967, 307 aa; fasta scores: opt: 146 Z-score: 160.2 bits: 38.4 E(): 0.26; 26.590% identity in 173 aa overlap 3 2 18 2 [Reporter]SCO7707 (20C23)_PCR/3399 [Gene]SCO7707/3095 NC_003888 SCBAC12C8.08, conserved hypothetical protein, len: 94aa: similar to TR:AAK64828 (EMBL:AE007210) hypothetical protein from Rhizobium meliloti pSymA Megaplasmid (83 aa) fasta scores; opt: 346, Z-score: 453.2, 65.753% identity (65.753% ungapped) in 73 aa overlap. 3 2 17 22 [Reporter]SCO1121 (1C19)_PCR/3397 [Gene]SCO1121/3094 NC_003888 2SCG38.14, possible secreted protein, len: 246 aa; highly similar to TR:Q9XAG0 (EMBL:AL079356) Streptomyces coelicolor putative secreted protein SC6G9.22, 248 aa; fasta scores: opt: 1111 z-score: 1110.0 E(): 0; 66.8% identity in 244 aa overlap. Contains possible N-terminal region signal peptide sequence 3 2 17 20 [Reporter]SCO6814 (3K15)_PCR/3395 [Gene]SCO6814/3093 NC_003888 SC1A2.23c, possible ABC transport protein ATP-binding component, len: 223 aa. Similar to many ABC transporters including: Deinococcus radiodurans TR:AAF12204 (EMBL:AE001862) phosphate ABC transporter, ATP-binding protein (254 aa), fasta scores opt: 458 z-score: 524.0 E(): 8.7e-22 38.1% identity in 210 aa overlap and Escherichia coli SW:PSTB_ECOLI (EMBL:X02723) phosphate ABC transporter, ATP-binding protein, PstB(257 aa), fasta scores opt: 436 z-score: 499.3 E(): 2.1e-20 38.9% identity in 198 aa overlap. Contains Prosite hits to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Also contains a Pfam match to entry PF00005 ABC_tran, ABC transporter. 3 2 17 19 [Reporter]SCO4829 (4G15)_PCR/3394 [Gene]SCO4829/3092 NC_003888 SC2A6.14, probable oxidoreductase, len: 510 aa; similar to SW:ALKJ_PSEOL (EMBL:X65936) Pseudomonas oleovorans alcohol dehydrogenase [acceptor] (EC 1.1.99.-) AlkJ, 558 aa; fasta scores: opt: 474 z-score: 541.0 E(): 1.2e-22; 31.1% identity in 541 aa overlap. Contains Pfam match to entry PF00732 GMC_oxred, GMC oxidoreductases and match to Prosite entry PS00624 GMC oxidoreductases signature 2 3 2 17 18 [Reporter]SCO2385 (5C15)_PCR/3393 [Gene]SCO2385/3091 NC_003888 SC4A7.13c, hypothetical protein, len: 218 aa; similar to various hypothetical proteins, e.g. TR:Y181_MYCTU (EMBL:Z97050) Mycobacterium tuberculosis hypothetical 26.3 kD protein MCTI28.21c, 244 aa; fasta scores: opt: 410 z-score: 496.1 E(): 3e-20; 39.0% identity in 236 aa overlap 3 2 17 17 [Reporter]SCO6536 (6O11)_PCR/3392 [Gene]SCO6536/3090 NC_003888 SC5C7.21c, unknown, len: 271 aa; similar to two S. coelicolor hypothetical proteins SC1F2.04 (E(): 0, 51.3% identity in 269 aa overlap) and SC1F2.03c (E(): 4.5e-21, 44.7% identity in 275 aa overlap) 4 3 11 21 [Reporter]SCO1580 (2H10)_PCR/5784 [Gene]SCO1580/5269 NC_003888 SCI35.02c, argC, N-acetyl-gamma-glutamyl-phosphate reductase, len: 342 aa; identical to ARGC_STRCO N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38) (249 aa). Contains PS01224 N-acetyl-gamma-glutamyl-phosphate reductase active site. Referred to as 'argA' on genetic map. 4 3 11 20 [Reporter]SCO4087 (3D10)_PCR/5783 [Gene]SCO4087/5268 NC_003888 SCD25.23, purM, phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), len: 355 aa. Identical to Streptomyces lividans TR:Q9ZB03 (EMBL:U64826) phosphoribosyl aminoimidazole synthetase (EC 6.3.3.1) (355 aa), fasta scores opt: 2313 z-score: 2645.5 E(): 0; 100.0% identity in 355 aa overlap and highly similar to many others e.g. Escherichia coli SW:PUR5_ECOLI (EMBL:M13747) phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1) (AIRS) (phosphoribosyl-aminoimidazole synthetase) (AIR synthase) (344 aa), fasta scores opt: 1018 z-score: 1167.8 E(): 0; 48.4% identity in 343 aa overlap. Contains a Pfam match to entry PF00586 AIRS, AIR synthase related proteins. 4 3 11 19 [Reporter]SCO2240 (4P6)_PCR/5782 [Gene]SCO2240/5267 NC_003888 SC1G2.02c, possible membrane protein, len: 222 aa. Contains a possible N-terminal membrane spanning hydrophobic domain. 4 3 11 18 [Reporter]SCO2196 (5L6)_PCR/5781 [Gene]SCO2196/5266 NC_003888 SC5F7.05, possible integral membrane protein, len: 234aa; similar to SW:Y00U_MYCTU hypothetical protein from Mycobacterium tuberculosis (250 aa) fasta scores; opt: 574, z-score: 642.0, E(): 2e-28, (39.4% identity in 226 aa overlap). Contains possible membrane spanning hydrophobic regions. 4 3 11 16 [Reporter]SCO7274 (7D6)_PCR/5779 [Gene]SCO7274/5265 NC_003888 SC5H1.18, possible membrane protein, len: 400 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains hydrophobic, probable membrane-spanning regions 4 3 11 15 [Reporter]SCO5573 (8P2)_PCR/5778 [Gene]SCO5573/5264 NC_003888 SC7A1.17, fpg, formamidopyrimidine-DNA glycosylase, len: 286aa; similar to many eg. TR:O69470 (EMBL:AL023635) putative formamidopyrimidine-DNA glycosylase from Mycobacterium leprae (282 aa) fasta scores; opt: 1075, z-score: 1367.9, E(): 0, (59.8% identity in 286 aa overlap) and SW:FPG_ECOLI formamidopyrimidine-DNA glycosylase from Escherichia coli(269 aa) fasta scores; opt: 508, z-score: 712.6, E(): 2.1e-32, (36.2% identity in 282 aa overlap). Contains Pfam match to entry PF01149 Fapy_DNA_glyco, Formamidopyrimidine-DNA glycosylase, score 390.10, E-value 2.2e-113. 4 3 11 14 [Reporter]SCO2651 (9L2)_PCR/5777 [Gene]SCO2651/5263 NC_003888 SC8E4A.21c, possible dehydrogenase/reductase (putative secreted protein), len: 245 aa; similar to TR:CAB52925 (EMBL:AL109949) Streptomyces coelicolor putative oxidoreductase SCJ11.40, 250 aa; fasta scores: opt: 717 z-score: 778.8 E(): 0; 47.6% identity in 250 aa overlap and to SW:LINX_PSEPA (EMBL:D23722) Pseudomonas paucimobilis 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (EC 1.1.-.-) LinX, 250 aa; fasta scores: opt: 446 z-score: 488.9 E(): 8e-20; 34.3% identity in 248 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain. Contains possible N-terminal region signal peptide sequence dehydrogenases/reductases family signature 4 3 11 13 [Reporter]SCO6489 (10H2)_PCR/5776 [Gene]SCO6489/5262 NC_003888 SC9C7.25, conserved hypothetical protein, len: 310 aa; similar to several proteins of undefined function egs. TR:P74220 (EMBL:D90913) hypothetical protein from Synechocystis sp. (strain PCC 6803) (301 aa) fasta scores; opt: 642, z-score: 649.7, E(): 6.7e-29, (38.8% identity in 309 aa overlap) and TR:Q47511 (EMBL:X57583) MccF protein from Escherichia coli plasmid pMCCC7 thought to be involved in self-immunity to translation inhibitor microcin C7 (344 aa) fasta scores; opt: 178, z-score: 259.5, E(): 3.6e-07, (25.1% identity in 339 aa overlap) 3 2 17 16 [Reporter]SCO5662 (7K11)_PCR/3391 [Gene]SCO5662/3089 NC_003888 SC6A9.05, possible adenosine deaminase, len: 387 aa; similar to many e.g. ADD_ECOLI adenosine deaminase (EC 3.5.4.4) (333 aa), fasta SCORES; opt: 520 z-score: 510.9 E(): 3.2e-21, 31.3% identity in 335 aa overlap 4 3 11 12 [Reporter]SCO2713 (11D2)_PCR/5775 [Gene]SCO2713/5261 NC_003888 SCC61A.34, unknown, len: 382aa; 3 2 17 15 [Reporter]SCO5299 (8G11)_PCR/3390 [Gene]SCO5299/3088 NC_003888 SC6G9.34, possible membrane protein, len: 312 aa; unknown function, similar to proteins from Mycobacterium leprae and Mycobacterium tuberculosis e.g. TR:O33063 (EMBL:Z99494) Mycobacterium leprae hypothetical protein (341 aa), fasta scores; opt: 316 z-score: 387.1 E(): 3.1e-14, 27.1% identity in 255 aa overlap (divergent at C-terminus). Contains hydrophobic, possible membrane-spanning region 4 3 11 11 [Reporter]SCO4428 (11P22)_PCR/5774 [Gene]SCO4428/5260 NC_003888 SCD6.06c, possible secreted protein, len: 507 aa. Contains possible N-terminal region signal peptide sequence 3 2 17 14 [Reporter]SCO5512 (9C11)_PCR/3389 [Gene]SCO5512/3087 NC_003888 SC8D9.24, ilvB, acetolactate synthase, len: 613aa; high level of similarity to many eg. TR:Q59816 (EMBL:L39268) ilvB, acetolactate synthase from the ilvBNC gene cluster of Streptomyces avermitilis (617 aa) fasta scores; opt: 3558, z-score: 3786.3, E(): 0, (87.2% identity in 619 aa overlap). Contains PS00187 Thiamine pyrophosphate enzymes signature and Pfam match to entry PF00205 TPP_enzymes, Thiamine pyrophosphate enzymes, score 899.00, E-value 3.2e-278. 3 2 17 13 [Reporter]SCO2512 (10O7)_PCR/3388 [Gene]SCO2512/3086 NC_003888 SCC121.15c, unknown, len: 68 aa 3 2 17 12 [Reporter]SCO4387 (11K7)_PCR/3387 [Gene]SCO4387/3085 NC_003888 SCD10.19c, probable pyridoxamine oxidase, len: 234 aa; similar to SW:PDXH_ECOLI (EMBL:M92351) Escherichia coli pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5) PdxH, 217 aa; fasta scores: opt: 717 z-score: 876.3 E(): 0; 49.1% identity in 212 aa overlap. Contains Pfam match to entry PF01243 Pyridox_oxidase, Pyridoxamine 5'-phosphate oxidase and match to Prosite entry PS01064 Pyridoxamine 5'-phosphate oxidase signature 3 2 17 11 [Reporter]SCO4461 (12G7)_PCR/3386 [Gene]SCO4461/3084 NC_003888 SCD65.04c, possible tetR-family transcriptional regulatory protein, len: 185 aa; similar to SW:BETI_RHIME (EMBL:U39940) Rhizobium meliloti regulatory protein BetI, 203 aa; fasta scores: opt: 193 z-score: 251.5 E(): 1.5e-06; 27.3% identity in 183 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 33..54 (+3.34 SD) 3 2 17 10 [Reporter]SCO3080 (13C7)_PCR/3385 [Gene]SCO3080/3083 NC_003888 SCE25.21, possible secreted protein, len: 269 aa. Contains possible N-terminal region signal peptide sequence 3 2 17 9 [Reporter]SCO3325 (14O3)_PCR/3384 [Gene]SCO3325/3082 NC_003888 SCE68.23c, conserved hypothetical protein, len: 355 aa; similar to hypothetical proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. SW:YV29_MYCTU (EMBL:Z77162) Mycobacterium tuberculosis hypothetical protein (358 aa), fasta scores; opt: 1281 z-score: 1288.1 E(): 0, 56.5% identity in 356 aa overlap. Similar to TR:O54097 (EMBL:AL021529), SC10A5.04, S.coelicolor hypothetical protein (295 aa) (64.4% identity in 278 aa overlap) 3 2 17 8 [Reporter]SCO1844 (15K3)_PCR/3383 [Gene]SCO1844/3081 NC_003888 SCI8.29c, possible aldolase, len: 240 aa. Similar to many aldolases e.g. Escherichia coli SW:FUCA_ECOLI (EMBL: M31059) L-fuculose phosphate aldolase (EC 4.1.2.17) (215 aa), fasta scores opt: 485 z-score: 552.3 E(): 2.1e-23 42.3% identity in 194 aa overlap. Contains a Pfam match to entry PF00596 Aldolase_II, Class II Aldolase and Adducin N-terminal domain. 3 2 17 7 [Reporter]SCO3766 (16G3)_PCR/3382 [Gene]SCO3766/3080 NC_003888 SCH63.13, hypothetical protein, len: 340 aa; highly similar to TR:Q9S1P4 (EMBL:AL109972) Streptomyces coelicolor hypothetical protein SCJ9A.26, 341 aa; fasta scores: opt: 1171 z-score: 1269.9 E(): 0; 61.8% identity in 340 aa overlap 4 3 11 10 [Reporter]SCO3538 (12L22)_PCR/5773 [Gene]SCO3538/5259 NC_003888 SCE2.19c, unknown, len: 247aa; 4 3 11 9 [Reporter]SCO3677 (13H22)_PCR/5772 [Gene]SCO3677/5258 NC_003888 SCH35.47, possible purine phosphoribosyltransferase, len: 165 aa; similar to many eg. TR:P74916 (EMBL:D37928) purine phosphoribosyltransferase from Thermus flavus (154 aa) fasta scores; opt: 222, z-score: 291.9, E(): 6.3e-09, 931.8% identity in 151 aa overlap). 4 3 11 8 [Reporter]SCO0335 (14D22)_PCR/5771 [Gene]SCO0335/5257 NC_003888 SCF12.14, putative secreted protein, len: 1044 aa 4 3 11 7 [Reporter]SCO3771 (15P18)_PCR/5770 [Gene]SCO3771/5256 NC_003888 SCH63.18c, probable secreted penicillin binding protein, len: 546 aa; similar to TR:P72405 (EMBL:U56256) Streptomyces clavuligerus high molecular weight group B penicillin binding protein PcbR, 551 aa; fasta scores: opt: 1063 z-score: 1122.1 E(): 0; 37.7% identity in 559 aa overlap. Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and possible N-terminal region signal peptide sequence 4 3 11 6 [Reporter]SCO1446 (16L18)_PCR/5769 [Gene]SCO1446/5255 NC_003888 SCL6.03, possible integral membrane protein, len: 122 aa. Contains possible hydrophobic membrane spanning regions 4 3 11 5 [Reporter]SCO1196 (17H18)_PCR/5768 [Gene]SCO1196/5254 NC_003888 SCG11A.27c, putative secreted protein, len: 287 aa. Contains possible N-terminal region signal peptide sequence 4 3 11 4 [Reporter]SCO0566 (18D18)_PCR/5767 [Gene]SCO0566/5253 NC_003888 SCF73.13c, probable membrane protein, len: 94 aa. Contains possible hydrophobic membrane spanning region 4 3 11 3 [Reporter]SCO1983 (19P14)_PCR/5766 [Gene]SCO1983/5252 NC_003888 SC3C9.18c, conserved hypothetical protein, len: 229 aa; similar to TR:BAB49166 (EMBL:AP002998) Rhizobium loti MLR1911 protein, 249 aa; fasta scores: opt: 689 Z-score: 839.8 bits: 162.8 E(): 3.6e-39; 50.000% identity in 234 aa overlap. Contains a TTA leucine codon, possible target for bldA regulation 3 2 17 6 [Reporter]SCO1613 (17C3)_PCR/3381 [Gene]SCO1613/3079 NC_003888 SCI35.35c, possible glutamine synthetase, len: 462 aa; similar to many e.g. GLNA_PYRWO glutamine synthetase I (EC 6.3.1.2) (439 aa), fasta scores; opt: 536 z-score: 653.9 E(): 3.6e-29, 31.6% identity in 450 aa overlap. Contains Pfam match to entry PF00120 gln-synt, Glutamine synthetase, score 123.40, E-value 9.4e-39 4 3 11 2 [Reporter]SCO3050 (20H14)_PCR/5765 [Gene]SCO3050/5251 NC_003888 SCBAC19G2.05c, unknown, len: 346aa: no significant database matches. 3 2 17 5 [Reporter]SCO0926 (17O23)_PCR/3380 [Gene]SCO0926/3078 NC_003888 SCM10.14c, hypothetical protein, len: 299 aa; similar to TR:CAB58282 (EMBL:AL121850) Streptomyces coelicolor conserved hypothetical protein SCC78.01c (fragment), 234 aa; fasta scores: opt: 221 z-score: 264.8 E(): 2.3e-07; 30.0% identity in 250 aa overlap 4 3 10 22 [Reporter]SCO2794 (1H10)_PCR/5763 [Gene]SCO2794/5250 NC_003888 2SCC13.02c, lacI-family transcriptional regulatory protein, len: 351 aa; highly similar to TR:Q9X9R3 (EMBL:AJ009798) Streptomyces reticuli CebR protein, 350 aa; fasta scores: opt: 2011 z-score: 2174.4 E(): 0; 89.4% identity in 350 aa overlap and C-terminal region identical to TR:BAB03462 (EMBL:AB036424) Streptomyces coelicolor transcriptional regulator (fragment) ORF5-H, 193 aa. Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and match to Prosite entry PS00356 Bacterial regulatory proteins, lacI family signature. Also contains a possible helix-turn-helix motif at residues 14..35 (+6.05 SD) 3 2 17 4 [Reporter]SCP1.229c (18K23)_PCR/3379 [Gene]SCP1.229c/3077 NC_003888 SCP1.229c, mmyY, hypothetical protein, len: 153aa; previously partially sequenced and annotated as TR:Q9JN76 (EMBL:AJ276673). 3 2 17 3 [Reporter]SCO1670 (19G23)_PCR/3378 [Gene]SCO1670/3076 NC_003888 SCI52.12, possible oxidoreductase, len: 328 aa; similar to TR:Q44328 (EMBL:AF242881) Agrobacterium radiobacter MocA protein, 354 aa; fasta scores: opt: 708 Z-score: 822.0 bits: 160.5 E(): 3.3e-38; 41.246% identity in 337 aa overlap 3 2 17 2 [Reporter]SCO7710 (20O19)_PCR/3377 [Gene]SCO7710/3075 NC_003888 SC8D11.01, unknown (fragment), len: >37 aa,SCBAC12C8.11, possible phosphotransferase, len: 248 aa: similar to SW:P18623 (VPH_STRVI) viomycin resistance phosphotransferase Vph from Streptomyces vinaceus (287 aa) fasta scores; opt: 17,3 Z-score: 197.2, 31.527% identity (37.870% ungapped) in 203 aa overlap. 3 2 16 22 [Reporter]SCO1222 (1O15)_PCR/3375 [Gene]SCO1222/3074 NC_003888 2SCG58.22, conserved hypothetical protein, len: 288 aa; similar to TR:P71889 (EMBL:Z79702) Mycobacterium tuberculosis hypothetical 33.0 kD protein MTCY3G12.11, 302 aa; fasta scores: opt: 561 z-score: 623.2 E(): 3e-27; 47.0% identity in 279 aa overlap 3 2 16 21 [Reporter]SCO5554 (2K15)_PCR/3374 [Gene]SCO5554/3073 NC_003888 SC1C2.35, leuD, 3-isopropylmalate dehydratase small subunit, len: 197 aa; highly similar to many e.g. LEUD_ECOLI 3-isopropylmalate dehydratase small subunit (201 aa), fasta scores; opt: 628 z-score: 1023.1 E(): 0, 52.3% identity in 197 aa overlap. Contains Pfam match to entry PF00694 Aconitase_C, Aconitase C-terminal domain, score 149.00, E-value 8.4e-41 3 2 16 20 [Reporter]SCO7794 (3G15)_PCR/3373 [Gene]SCO7794/3072 NC_003888 SC10B8A.04, possible TetR-family transcriptional regulator, len: 212 aa; similar to many e.g. SW:P03039 (TER3_ECOLI) tetracycline repressor protein class C from Escherichia coli (219 aa) fasta scores; opt: 358, z-score: 432.3, E(): 1.3e-16, 36.4% identity in 217 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and helix-turn-helix motif at residue 26-47 (Score 1166 (+3.16 SD)). 3 2 16 19 [Reporter]SCO4820 (4C15)_PCR/3372 [Gene]SCO4820/3071 NC_003888 SC2A6.05c, possible serine/threonine protein kinase, len: 712 aa; C-terminal region similar to TR:O53510 (EMBL:AL021957) Mycobacterium tuberculosis serine/threonine protein kinase MTV021.09, 399 aa; fasta scores: opt: 289 z-score: 239.4 E(): 7.5e-06; 30.6% identity in 340 aa overlap and to Streptomyces coelicolor SC2A6.02c, 452 aa; fasta scores: opt: 1410 z-score: 964.7 E(): 0; 68.7% identity in 339 aa overlap. Contains 2 Pfam matches to entry PF00069 pkinase, Eukaryotic protein kinase domain. Contains also possible hydrophobic membrane spanning regions, 3x degenerate repeat: (T/H)GTGTGT(8)AHPA(K/E)GDTD(N/D)PG(16) and 2x degenerate repeat: PSRA(Q/H)TDTAHPAEGDTD(D/N)PGHTSGNGTTGTDGT(R/G)S(7) 3 2 16 18 [Reporter]SCO2104 (5O11)_PCR/3371 [Gene]SCO2104/3070 NC_003888 SC4A10.37c, possible thiamin phosphate pyrophosphorylase, len: 223 aa; similar to TR:O34294 (EMBL:AF004408), thiE, from Rhizobium etli plasmid pb (204 aa), fasta scores; opt: 251 z-score: 300.4 E(): 2.1e-09, 35.5% identity in 183 aa overlap. Also similar to many hypothetical thiamin phosphate pyrophosphorylases e.g. SW:THIE_MYCTU (EMBL:Z84724), thiE, Mycobacterium tuberculosis probable thiamin-phosphate pyrophosphorylase (63.0% identity in 219 aa overlap) 4 3 10 21 [Reporter]SCO1514 (2D10)_PCR/5762 [Gene]SCO1514/5249 NC_003888 SCL2.04c, apt, adenine phosphoribosiltransferase, len: 182 aa; identical to previously sequenced SW:APT_STRCO (EMBL:X87267) Streptomyces coelicolor adenine phosphoribosyltransferase (EC 2.4.2.7) Apt, 182 aa and highly smilar to SW:APT_ECOLI (EMBL:M14040) Escherichia coli adenine phosphoribosyltransferase (EC 2.4.2.7) Apt, 183 aa; fasta scores: opt: 600 z-score: 682.8 E(): 1.2e-30; 54.8% identity in 168 aa overlap. Contains Pfam match to entry PF00156 Pribosyltran, Phosphoribosyl transferase domain and match to Prosite entry PS00103 Purine/pyrimidine phosphoribosyl transferases signature 4 3 10 20 [Reporter]SCO4735 (3P6)_PCR/5761 [Gene]SCO4735/5248 NC_003888 SC6G4.13, rpsI, 30S ribosomal protein S9, len: 170 a a; identical to RS9_STRCO 30S ribosomal protein S9 (170 aa) and highly similar to many e.g.RS9_ECOLI (129 aa), fasta s cores; opt: 408 z-score: 663.5 E(): 1.1e-29, 49.2% identity in 124 aa overlap. Contains PS00360 Ribosomal protein S9 s ignature and Pfam match to entry PF00380 S9, Ribosomal prot ein S9, score 162.60, E-value 1e-49 4 3 10 19 [Reporter]SCO6625 (4L6)_PCR/5760 [Gene]SCO6625/5247 NC_003888 SC1F2.22, unknown, len: 221 aa 4 3 10 18 [Reporter]SCO5727 (5H6)_PCR/5759 [Gene]SCO5727/5246 NC_003888 SC3C3.13, unknown, len: 200 aa 4 3 10 17 [Reporter]SCO2043 (6D6)_PCR/5758 [Gene]SCO2043/5245 NC_003888 SC4G6.12c, trpE3, probable anthranilate synthase component I, len: 502aa; similar to many eg. SW:TRPE_ARTGO anthranilate synthase component I from Arthrobacter globiformis (531 aa) fasta scores; opt: 1921, z-score: 2150.9, E(): 0, (60.6% identity in 500 aa overlap). Contains Pfam match to entry PF00425 chorismate_bind, chorismate binding enzyme. 4 3 10 16 [Reporter]SCO3609 (7P2)_PCR/5757 [Gene]SCO3609/5244 NC_003888 SC66T3.20, possible membrane protein, len: 623 aa; unknown function, C-terminus shares weak similarity with other hypothetical membrane proteins e.g. TR:P74405 (EMBL:D90914) Synechocystis sp. hypothetical protein (568 aa), fasta scores; opt: 250 z-score: 272.0 E(): 8e-08, 24.3% identity in 576 aa overlap. Contains hydrophobic, possible membrane-spanning regions 4 3 10 15 [Reporter]SCO5569 (8L2)_PCR/5756 [Gene]SCO5569/5243 NC_003888 SC7A1.13, unknown, len: 379 aa; similar to TR:O69467 (EMBL:AL023635) hypothetical protein from Mycobacterium tuberculosis (245 aa) fasta scores; opt: 254, z-score: 388.8, E(): 2.3e-14, (29.5% identity in 234 aa overlap). Contains probable coiled-coil from 82 to 124 (43 residues) Max score: 1.615 (probability 1.00). 4 3 10 14 [Reporter]SCO5674 (9H2)_PCR/5755 [Gene]SCO5674/5242 NC_003888 SC8B7.04, probable integral membrane protein, len: 247 aa. Contains possible hydrophobic membrane spanning regions 3 2 16 17 [Reporter]SCO6528 (6K11)_PCR/3370 [Gene]SCO6528/3069 NC_003888 SC5C7.13, probable integral membrane transport protein, len: 474 aa; similar to many e.g. ARCD_PSEAE arginine/ornithine antiporter (482 aa), fasta scores; opt: 560 z-score: 470.6 E(): 5.8e-19, 28.9% identity in 439 aa overlap 4 3 10 13 [Reporter]SCO6485 (10D2)_PCR/5754 [Gene]SCO6485/5241 NC_003888 SC9C7.21, unknown, len: 240 aa 3 2 16 16 [Reporter]SCO5701 (7G11)_PCR/3369 [Gene]SCO5701/3068 NC_003888 SC5H4.25c hypothetical protein, len: 167 aa; similar to TR:O05815 (EMBL:Z95207) Mycobacterium tuberculosis hypothetical 16.9 kDp protein MTCY24A1.13c, 162 aa; fasta scores: opt: 242 z-score: 266.6 E(): 2.2e-07; 38.9% identity in 144 aa overlap 4 3 10 12 [Reporter]SCO2767 (10P22)_PCR/5753 [Gene]SCO2767/5240 NC_003888 SCC57A.38c, possible ATP/GTP-binding protein, len: 743 aa. Weakly similar to another Streptomyces coelicolor TR:O86768(EMBL:AL031035) putative ATP/GTP-binding protein (886 aa), fasta scores opt: 328 z-score: 356.5 E(): 1.8e-12 28.9% identity in 699 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 3 2 16 14 [Reporter]SCO6979 (9O7)_PCR/3367 [Gene]SCO6979/3067 NC_003888 SC8F11.05, probable solute-binding lipoprotein, len: 340 aa. Highly similar to many sugar transport proteins including Bacillus subtilis SW:RBSB_BACSU(EMBL:Z92953) D-ribose-binding protein precursor, RbsB (305 aa), fasta scores opt: 222 z-score: 243.9 E(): 3.7e-06 23.0% identity in 256 aa overlap and several examples from Streptomyces coelicolor e.g. TR:Q9X837(EMBL:AL049727) putative secreted solute binding protein, SC9B1.17C (337 aa), fasta scores opt: 1281 z-score: 1360.6 E():0 59.1% identity in 328 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 3 2 16 13 [Reporter]SCO2507 (10K7)_PCR/3366 [Gene]SCO2507/3066 NC_003888 SCC121.10, probable metal transport ABC transporter, len: 300 aa; similar to TR:Q9XDA5 (EMBLAF104349:) Listeria monocytogenes hydrophobic membrane protein ZurM, 295aa fasta scores: opt: 608 z-score: 697.8 E(): 1.8e-31; 34.7% identity in 288 aa overlap and to SW:ZNUB_ECOLI (EMBL:AE000280) Escherichia coli high-affinity Zinc uptake system membrane protein ZnuB, 261 aa; fasta scores: opt: 397 z-score: 459.6 E(): 3.3e-18; 27.3% identity in 245 aa overlap. Contains Pfam match to entry PF00950 ABC-3 3 2 16 12 [Reporter]SCO2541 (11G7)_PCR/3365 [Gene]SCO2541/3065 NC_003888 SCC77.08c, probable transmembrane transport protein, len: 456 aa. Highly similar to many including: Pseudomonas putida SW:PHT1_PSEPU(EMBL:D13229) phthalate transporter (451 aa), fasta scores opt: 817 z-score: 912.4 E():0 34.6% identity in 416 aa overlap, Streptomyces coelicolor TR:CAB61275(EMBL:AL132991) putative transporter protein SCF55.05C (455 aa), fasta scores opt: 699 z-score: 781.2 E():0 36.0% identity in 453 aa overlap and Agrobacterium vitis SW:TUB4_AGRVI(EMBL:U25634) putative tartrate transporter (433 aa), fasta scores opt: 1154 z-score: 1287.3 E():0 43.6% identity in 433 aa overlap. Contains a Pfam match to entry PF00083 sugar_tr and multiple possible membrane spanning hydrophobic domains. 3 2 16 11 [Reporter]SCO4815 (12C7)_PCR/3364 [Gene]SCO4815/3064 NC_003888 SCD63A.26c, possible secreted protein, len: 214 aa. Contains possible N-terminal signal peptide sequence 3 2 16 10 [Reporter]SCO3045 (13O3)_PCR/3363 [Gene]SCO3045/3063 NC_003888 SCE34.26, putative secreted protein, len: 507 aa; similar to TR:O68907 (EMBL:AF05830) Streptomyces roseofulvus FrnA, 770 aa; fasta scores: opt: 1350 z-score: 1461.9 E(): 0; 55.1% identity in 430 aa overlap, to Streptomyces coelicolor SCE34.24, 472 aa; fasta scores: opt: 2161 z-score: 2145.7 E(): 0; 70.9% identity in 471 aa overlap, to SCE34.25, 507 aa; fasta scores: opt: 739 z-score: 735.6 E(): 0; 31.9% identity in 508 aa overlap, to SCE34.23, 404 aa; fasta scores: opt: 709 z-score: 707.2 E(): 7e-34; 36.4% identity in 357 aa overlap and to SCE34.27 (fragment), >356 aa; fasta scores: opt: 643 z-score: 642.5 E(): 2.8e-30; 39.5% identity in 354 aa overlap. Contains possible N-terminal region signal peptide sequence 3 2 16 9 [Reporter]SCO1645 (14K3)_PCR/3362 [Gene]SCO1645/3062 NC_003888 SCI41.28c, hypothetical protein, len: 197 aa. Identical to TR:O87597 (EMBL:AF086832) Streptomyces coelicolor hypothetical 18.6 kD protein and similar to SW:VG59_BPMD2 (EMBL:AF022214) Mycobacteriophage D29 gene 59 protein, 153 aa; fasta scores: opt: 175 z-score: 225.0 E(): 3.7e-05; 35.2% identity in 122 aa overlap 3 2 16 8 [Reporter]SCO3645 (15G3)_PCR/3361 [Gene]SCO3645/3061 NC_003888 SCH10.23c, probable hydrolase, len: 410 aa; similar to e.g. SW:KYNU_RAT (EMBL:U68168) Rattus norvegicus L-kynurenine hydrolase (464 aa), fasta scores; opt: 458 z-score: 408.3 E(): 2e-15, 27.9% identity in 462 aa overlap 3 2 16 7 [Reporter]SCO1602 (16C3)_PCR/3360 [Gene]SCO1602/3060 NC_003888 SCI35.24c, unknown, len: 243 aa; possible truncated at N-terminus by IS117 4 3 10 11 [Reporter]SCO4262 (11L22)_PCR/5752 [Gene]SCO4262/5239 NC_003888 SCD49.03, unknown, len: 115 aa; doubtful CDS. Contains a TTA leucine codon, possible target for bldA regulation and high %G+C (70.43) 4 3 10 10 [Reporter]SCO3389 (12H22)_PCR/5751 [Gene]SCO3389/5238 NC_003888 SCE126.07c, probable two component system response regulator, len: 224aa; similar to many, particularly others from Streptomyces coelicolor eg. TR:O86632 (EMBL:AL031155) putative two component regulator (222 aa) fasta scores; opt: 784, z-score: 897.1, E(): 0, (57.7% identity in 215 aa overlap). Also similar to SW:DEGU_BACSU transcriptional regulator protein DegU from Bacillus subtilis (229 aa) fasta scores; opt: 466, z-score: 538.0, E(): 1.2e-22, (37.2% identity in 223 aa overlap). Contains Pfam matches to entry PF00072 response_reg, Response regulator receiver domain and to entry PF00196 GerE, Bacterial regulatory proteins, luxR family. Also contains Prosite match to PS00622 Bacterial regulatory proteins, luxR family signature. 4 3 10 9 [Reporter]SCO3341 (13D22)_PCR/5750 [Gene]SCO3341/5237 NC_003888 SCE7.08c, possible integral membrane protein, len: 167aa; Contains possible membrane spanning hydrophobic regions. 4 3 10 8 [Reporter]SCO4873 (14P18)_PCR/5749 [Gene]SCO4873/5236 NC_003888 SCK20.14c, unknown, len: 312 aa 4 3 10 7 [Reporter]SCO3555 (15L18)_PCR/5748 [Gene]SCO3555/5235 NC_003888 SCH5.18c, possible integral membrane protein, len: 321 aa; similar to TR:O69626 (EMBL:AL022121) hypothetical protein (266 aa) fasta scores; opt: 335, z-score: 348.0, E(): 4.5e-12, (38.1% identity in 247 aa overlap). Contains probable coiled-coil from 227 to 264 (38 residues), max score: 1.545, (probability 0.98). 4 3 10 6 [Reporter]SCO0338 (16H18)_PCR/5747 [Gene]SCO0338/5234 NC_003888 SCF12.17c, possible dehydrogenase, len: 179 aa; similar to the C-terminal many proposed glucose-6-phosphate dehydrogenase eg. TR:AAD36231 (EMBL:AE001772) from Thermotoga maritima (496 aa) fasta scores; opt: 314, z-score: 381.8, E(): 6.5e-14, (42.5% identity in 146 aa overlap). Contains Pfam match to entry PF00479 G6PD, Glucose-6-phosphate dehydrogenase. 4 3 10 5 [Reporter]SCO0198 (17D18)_PCR/5746 [Gene]SCO0198/5233 NC_003888 SCJ12.10c, unknown, len: 288 aa. Similar to several hypothetical proteins including: Mycobacterium tuberculosis SW:YW12_MYCTU (EMBL; Z74025) hypothetical 30.9 KD protein MTCY39.12 (295 aa), fasta scores opt: 363 z-score: 441.0 E(): 3.3e-17 32.3% identity in 288 aa overlap and Mycobacterium tuberculosis TR:O06189 (EMBL; Z95387) hypothetical 31.7 KD protein MTCY1A10.09C (297 aa), fasta scores opt: 336 z-score: 408.6 E(): 2.1e-15 29.3% identity in 294 aa overlap. Also highly similar to S. coelicolor hypothetical proteins (AL109962) SCJ1.29c (283 aa), fasta scores opt: 1161 z-score: 1097.2 E(): 0 63.5% identity in 288 aa overlap and SCJ1.16c (294 aa), fasta scores opt:892 z-score: 844.7 E():0 50.3% identity in 296 aa overlap 4 3 10 4 [Reporter]SCO6091 (18P14)_PCR/5745 [Gene]SCO6091/5232 NC_003888 SCBAC1A6.15c, probable integral membrane protein, len: 756 aa; similar to SW:MMLB_MYCTU (EMBL:AL021928) Mycobacterium tuberculosis putative membrane protein MmpL11 or MTV033.10c, 966 aa; fasta scores: opt: 1132 z-score: 1196.3 E(): 0; 32.6% identity in 723 aa overlap. Contains possible hydrophobic membrane spanning regions 3 2 16 6 [Reporter]SCO0105 (16O23)_PCR/3359 [Gene]SCO0105/3059 NC_003888 SCJ11.34c, xlnC, endo-1,4-beta-xylanase (putative secreted protein), len: 241 aa; highly similar to many endo-1,4-beta-xylanases e.g. SW:XYNC_STRLI from Streptomyces lividans (240 aa) fasta scores; opt: 1605, z-score: 1785.1, E(): 0, (97.9% identity in 241 aa overlap) and SW:XYNA_BACSU from Bacillus subtilis (213 aa) fasta scores; opt: 813, z-score: 910.7, E(): 0, (58.8% identity in 221 aa overlap). Contains Pfam match to entry PF00457 Glyco_hydro_11, Glycosyl hydrolases family 11 and Prosite matches to PS00777 Glycosyl hydrolases family 11 active site signature 2 and PS00776 Glycosyl hydrolases family 11 active site signature 1 4 3 10 3 [Reporter]SCO6101 (19L14)_PCR/5744 [Gene]SCO6101/5231 NC_003888 SCBAC1A6.25c, unknown, len: 59 aa 3 2 16 5 [Reporter]SCO0869 (17K23)_PCR/3358 [Gene]SCO0869/3058 NC_003888 SCM1.02c, possible anti-sigma factor antagonist, len: 124 aa. Weakly similar to several putative anti anti sigma factors including: Bacillus subtilis SW:SP21_BACSU( EMBL:M17643) anti-sigma F factor antagonist (stage II sporulation protein AA) (116 aa), fasta scores opt: 147 z-score: 204.6 E(): 0.00047 26.6% identity in 109 aa overlap and Streptomyces coelicolor A3(2) TR:CAB55835 (EMBL:AJ249581) putative anti anti sigma factor (125 aa), fasta scores opt: 142 z-score: 197.8 E(): 0.0011 29.8% identity in 94 aa overlap. Contains a Pfam match to entry PF01740 SpoIIAA, SpoIIAA family. 4 3 10 2 [Reporter]SCO2820 (20D14)_PCR/5743 [Gene]SCO2820/5230 NC_003888 SCBAC17F8.11c, hypothetical protein, len: 115 aa: no significant database matches. 3 2 16 4 [Reporter]SCO1891 (18G23)_PCR/3357 [Gene]SCO1891/3057 NC_003888 SCI7.09c, hypothetical protein, len: 199 aa; similar to TR:P71985 (EMBL:Z81360) hypothetical protein from Mycobacterium tuberculosis (189 aa) fasta scores; opt: 210, z-score: 248.3, E(): 1.7e-06, (32.4% identity in 182 aa overlap). 3 2 16 3 [Reporter]SCP1.98 (19C23)_PCR/3356 [Gene]SCP1.98/3056 NC_003888 SCP1.98, possible large ala-rich protein, len: 723aa; N-terminal half contains multiple possible coiled-coil regions. 3 2 16 2 [Reporter]SCO7509 (20K19)_PCR/3355 [Gene]SCO7509/3055 NC_003888 SCBAC25F8.01c, conserved hypothetical protein (fragment), len: >291 aa; similar to TR:AAK48168 (EMBL:AE007177) Mycobacterium tuberculosis CDC1551 hypothetical 56.9 kDa protein MT3801, 509 aa; fasta scores: opt: 1078 Z-score: 1264.7 bits: 242.8 E(): 7.8e-63; 52.577% identity in 291 aa overlap and to TR:CAC32297 (EMBL:AL583943) Streptomyces coelicolor conserved hypothetical protein SC10A9.15c, 506 aa; fasta scores: opt: 1071 Z-score: 1149.0 bits: 221.3 E(): 2.8e-58; 54.639% identity in 291 aa overlap,SCBAC17A6.42c, conserved hypothetical protein, len: >234aa; similar to C-terminal region of TR:Q9AK89 (EMBL:AL583943) conserved hypothetical protein from Streptomyces coelicolor (505 aa) fasta scores: opt: 907, Z-score: 1080.6, 55.649% identity (57.826% ungapped) in 239 aa overlap and TR:O69666 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (509 aa) fasta scores: opt: 904, Z-score: 1077.0, 57.511% identity (58.772% ungapped) in 233 aa overlap. 3 2 15 22 [Reporter]SCO1218 (1K15)_PCR/3353 [Gene]SCO1218/3054 NC_003888 2SCG58.18c, possible transmembrane transport protein, len: 611 aa; similar to TR:Q9RX38 (EMBL:AE001907) Deinococcus radiodurans sodium:solute symporter protein DR0477, 528 aa; fasta scores: opt: 379 z-score: 407.0 E(): 3.3e-15; 30.5% identity in 564 aa overlap. Contains Pfam match to entry PF00474 SSF, Sodium:solute symporter family and match to Prosite entry PS00457 Sodium:solute symporter family signature 2. Also contains possible hydrophobic membrane spanning regions and 4x conserved repeat unit: RTDLL 3 2 15 21 [Reporter]SCO0962 (2G15)_PCR/3352 [Gene]SCO0962/3053 NC_003888 SCM11.17c, glgA, putative glycosyl transferase, len: 387 aa; identical to previously sequenced TR:Q9X9U5 (EMBL:AJ243803) Streptomyces coelicolor putative glycosyl transferase, GlgA, 387 and similar to TR:Q9X7Z1 (EMBL:AL049497) Streptomyces coelicolor putative glycosyl transferase SC6G10.05c, 412 aa; fasta scores: opt: 352 z-score: 421.1 E(): 4.4e-16; 29.3% identity in 403 aa overlap. Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1 3 2 15 20 [Reporter]SCO2227 (3C15)_PCR/3351 [Gene]SCO2227/3052 NC_003888 SC10B7.22c possible acetyltransferase, len: 186 aa; similar to TR:Q9XAQ0 (EMBL:AL078618) Streptomyces coelicolor putative acetyltransferase SC10A7.25, 187 aa; fasta scores: opt: 160 z-score: 203.0 E(): 0.00063; 29.9% identity in 157 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 3 2 15 19 [Reporter]SCO7025 (4O11)_PCR/3350 [Gene]SCO7025/3051 NC_003888 SC1H10.14c, probable LysR-family transcriptional regulator, len: 305 aa. Highly similar to the Streptomyces lividans TR:Q9ZI19 (EMBL:AF043654) putative LysR-type of transcriptional regulator, BxlS (300 aa), fasta scores opt: 1743 z-score: 1937.0 E(): 0 91.0% identity in 300 aa overlap. Also similar to many other putative LysR-family transcriptional regulators e.g. Streptomyces coelicolor TR:O86805 (EMBL:AL031317) putative transcriptional regulator, SC6G4.44 (306 aa), fasta scores opt: 479 z-score: 538.0 E(): 1.5e-22 38.3% identity in 300 aa overlap. Contains a Prosite hit to PS00044 Bacterial regulatory proteins, lysR family signature and a Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family with the potential helix-turn-helix motif situated between residues 16..37 (+4.86 SD). 3 2 15 18 [Reporter]SCO2100 (5K11)_PCR/3349 [Gene]SCO2100/3050 NC_003888 SC4A10.33, probable transcriptional regulator, len: 209 aa; shows weak similarity to many hypothetical proteins e.g. TR:O53641 (EMBL:AL021927) Mycobacterium tuberculosis hypothetical protein (214 aa), fasta scores; opt: 224 z-score: 282.1 E(): 2.2e-08, 28.8% identity in 208 aa overlap and to SCI30A.20c (EMBL:AL096811) S.coelicolor possible transcriptional regulator (215 aa) (31.6% identity in 190 aa overlap). Contains probable helix-turn-helix motif at aa 38-59 (Score 1454, +4.14 SD) and Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 4 3 9 22 [Reporter]SCO5274 (1D10)_PCR/5741 [Gene]SCO5274/5229 NC_003888 2SC7G11.36, unknown, len: 541 aa. High content in alanine amino acid residues and in G+C (77.57%). Also contains 3x degenerate repeat: DA(P/T)GA(W/R)(G/S)(T/S)(4) 4 3 9 20 [Reporter]SCO5624 (3L6)_PCR/5739 [Gene]SCO5624/5228 NC_003888 SC2E1.41, rpsB, 30S ribosomal protein S2, len: 310 aa; almost identical to RS2_STRCO 30S ribosomal protein S2 (242 aa), E(): 0, 96.7% identity in 242 aa overlap and also highly similar to many others e.g. RS2_ECOLI 30S ribosomal protein S2 (240 aa), fasta scores; opt: 744 z-score: 950.7 E(): 0, 50.4% identity in 224 aa overlap. Contains PS00962 Ribosomal protein S2 signature 1 and PS00211 ABC transporters family signature (probably a false positive). Also contains Pfam matches to entry S2 PF00318, Ribosomal protein S2, score 304.89 and to entry 60s_ribosomal PF00428, 60s Acidic ribosomal protein, score 24.36. Note that this sequence is longer than RS2_STRCO due to a sequence difference around 235 aa. This sequence has a C-terminal extension not present in other rpsB. The M. tuberculosis protein also has a C-terminal extension but with a differentsequence. Our sequence has been checked, and is also confirmed by sequence from the overlapping cosmid 6A9. 4 3 9 19 [Reporter]SCO6046 (4H6)_PCR/5738 [Gene]SCO6046/5227 NC_003888 SC1B5.06c, unknown, len: 249 aa 4 3 9 18 [Reporter]SCO5722 (5D6)_PCR/5737 [Gene]SCO5722/5226 NC_003888 SC3C3.08, serine protease (putative secreted protein), len: 413 aa; almost identical to TR:Q53863 (EMBL:U33176) serine protease (390 aa), 98.4% identity in 387 aa overlap and similar to many e.g. ELYA_BACSP alkaline elastase yab precursor (378 aa), fasta scores; opt: 466 z-score: 512.6 E(): 2.7e-21, 36.3% identity in 281 aa overlap. Also similar to downstream putative serine protease, SC3C3.17c (E(): 5.2e-07, 35.0% identity in 383 aa overlap). Contains PS00136, PS00137 and PS00138 Serine proteases, subtilase family, aspartic acid, histidine and serine active sites and Pfam match to entry PF00082 subtilase, Subtilase family of serine proteases, score 214.90, E-value 1.2e-60. Contains possible N-terminal region signal peptide sequence and possible hydrophobic membrane spanning region at C-terminal region 4 3 9 17 [Reporter]SCO6658 (6P2)_PCR/5736 [Gene]SCO6658/5225 NC_003888 SC5A7.08c, probable 6-phosphogluconate dehydrogenase, len: 293 aa; similar to many eukaryotic e.g. 6PGD_BACSU 6-phosphogluconate dehydrogenase, decarboxylating (468 aa), fasta sores; opt: 445 z-score: 616.2 E(): 4.4e-27, 36.6% identity in 287 aa overlap.also highly similar to S. coelicolor 6-phosphogluconate dehydrogenase (EMBL:L27063) TR:Q53917 (291 aa), fasta sores; opt: 1654 z-score: 2054.7 E(): 0, 83.4% identity in 289 aa overlap. Contains PS00065 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature and Pfam match to entry PF00393 6PGD, 6-phosphogluconate dehydrogenases, score 169.20, E-value 6.9e-47 4 3 9 16 [Reporter]SCO3605 (7L2)_PCR/5735 [Gene]SCO3605/5224 NC_003888 SC66T3.16, hypothetical protein, len: 98 aa; unknown function, probable CDS suggested by positional base preference and GC frame analysis. C-terminus is Asn-rich and contains 2x QQPY repeats 4 3 9 15 [Reporter]SCO2155 (8H2)_PCR/5734 [Gene]SCO2155/5223 NC_003888 SC6G10.28c, cox1, cytochrome c oxidase subunit I, len: 578aa; similar to many eg. SW:COX1_BACFI cytochrome c oxidase polypeptide I from Bacillus firmus (624 aa) fasta scores; opt: 1835, z-score: 2056.2, E(): 0, (49.5% identity in 527 aa overlap). Contains Pfam match to entry PF00115 COX1, Cytochrome C and Quinol oxidase polypeptide I and Prosite matches to PS00152 ATP synthase alpha and beta subunits signature and PS00077 Heme-copper oxidase catalytic subunit, copper B binding region signature. Also contains several probable membrane spanning hydrophobic regions. 4 3 9 13 [Reporter]SCO1502 (9P22)_PCR/5732 [Gene]SCO1502/5222 NC_003888 SC9C5.26c, possible secreted protein, len: 118 aa. Contains possible N-terminal signal peptide sequence 3 2 15 17 [Reporter]SCO5801 (6G11)_PCR/3348 [Gene]SCO5801/3049 NC_003888 SC4H2.22, unknown, len: 231 aa; some similarity to C-terminus of Saccharopolyspora erythraea malonyl CoA decarboxylase TR:Q54112 (EMBL:L05192) (417 aa), fasta scores; opt: 434 z-score: 630.6 E(): 6.6e-28, 40.5% identity in 190 aa overlap and similar to the pSymA plasmid borne TR:AAK65919 (EMBL:AE007311) Rhizobium meliloti (Sinorhizobium meliloti) RhbD rhizobactin siderophore biosynthesis protein, 196 aa; fasta scores: opt: 387 Z-score: 454.4 E(): 1.1e-17; 35.106% identity in 188 aa overlap 4 3 9 11 [Reporter]SCO4093 (11H22)_PCR/5730 [Gene]SCO4093/5221 NC_003888 SCD25.29c, possible integral membrane protein, len: 539 aa. Contains possible membrane spanning hydrophobic domains. 3 2 15 16 [Reporter]SCO5695 (7C11)_PCR/3347 [Gene]SCO5695/3048 NC_003888 SC5H4.19, possible metalloprotease, len: 430 aa; similar to TR:O83609 (EMBL:AE001235) Treponema pallidum zinc protease, putative TP0600, 450 aa; fasta scores: opt: 181 z-score: 205.4 E(): 0.00056; 28.0% identity in 400 aa overlap. Contains Pfam match to entry PF00595 PDZ, PDZ domain (Also known as DHR or GLGF) and match to Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature and also possible hydrophobic membrane spanning regions 4 3 9 10 [Reporter]SCO3385 (12D22)_PCR/5729 [Gene]SCO3385/5220 NC_003888 SCE126.03, possible lyase, len: 403aa; weakly similar to many eg. SW:LTAA_AERJA L-allo-threonine aldolase from Aeromonas jandaei DK-39 (338 aa) fasta scores; opt: 257, z-score: 293.4, E(): 5e-09, (31.4% identity in 255 aa overlap). 3 2 15 15 [Reporter]SCO2478 (8O7)_PCR/3346 [Gene]SCO2478/3047 NC_003888 SC7A8.17c, possible reductase, len: 195 aa; similar to TR:Q9ZGD0 (EMBL:AF080235) Streptomyces cyanogenus reductase homolog LanO, 193 aa; fasta scores: opt: 780 z-score: 927.8 E(): 0; 60.1% identity in 188 aa overlap and to TR:AAF00220 (EMBL:AF164961) Streptomyces fradiae UrdO, 197 aa; fasta scores: opt: 775 z-score: 921.8 E(): 0; 55.7% identity in 194 aa overlap 3 2 15 14 [Reporter]SCO6975 (9K7)_PCR/3345 [Gene]SCO6975/3046 NC_003888 SC8F11.01c, possible amino acid synthase, partial CDS, len: > 237 aa. Similar to several enzymes requiring TPP as a cofactor including: Spirulina platensis SW:ILVB_SPIPL(EMBL:M75907) acetolactate synthase (EC 4.1.3.18) IlvY (579 aa), fasta scores opt: 166 z-score: 195.5 E(): 0.0019 27.7% identity in 238 aa overlap. Also highly similar, in parts, to the Bacillus subtilis SW:IOLD_BACSU(EMBL:D14399) hypothetical protein, IolD found within the inositol utilisation operon (580 aa), fasta scores opt: 577 z-score: 669.1 E(): 7.7e-30 47.2% identity in 178 aa overlap. Contains a Pfam match to entry PF00205 TPP_enzymes, Thiamine pyrophosphate enzymes.,SC6F7.28c, probable acetolactate synthase, partial CDS, len: > 420 aa. Similar to many acetolactate synthases including: Mycobacterium tuberculosis SW:ILVB_MYCTU(EMBL:Z78020) probable acetolactate synthase (EC 4.1.3.18) (547 aa), fasta scores opt: 492 z-score: 552.0 E(): 2.6e-23 29.1% identity in 388 aa overlap. Also similar to Bacillus subtilis SW:IOLD_BACSU(EMBL:D14399) myo-inositol catabolic protein IolD (580 aa), fasta scores opt: 408 z-score: 458.5 E(): 4.3e-18 34.8% identity in 443 aa overlap. Contains a Pfam match to entry PF00205 TPP_enzymes, Thiamine pyrophosphate enzymes. 3 2 15 13 [Reporter]SCO6219 (10G7)_PCR/3344 [Gene]SCO6219/3045 NC_003888 SC2H4.01, probable ATP/GTP binding protein, partial CDS, len: >606 aa; contains PS00017 ATP /GTP-binding site motif A (P-loop),SC9G1.09, possible serine/threonine protein kinase (partial CDS), len: >688 aa; low similarity to TR:O54228 (EMBL:AJ223176) Streptomyces granaticolor ser/thr protein kinase (EC 2.7.1.37) (phosphorylase B kinase kinase) (glycogen synthase A kinase) (hydroxyalkyl-protein kinase) (serine(threonine) protein kinase), Pkg4, 761 aa; fasta scores: opt: 142 z-score: 146.7 E(): 0.84; 25.6% identity in 406 aa overlap. Contains possible hydrophobic membrane spanning region 3 2 15 12 [Reporter]SCO2537 (11C7)_PCR/3343 [Gene]SCO2537/3044 NC_003888 SCC77.04c, possible DNA-binding protein, len: 294 aa. Highly similar to several other Streptomyces coelicolor proteins including: TR:CAB52924 (EMBL:AL109949) putative DNA-binding protein SCJ11.39C (279 aa), fasta scores opt: 575 z-score: 702.8 E(): 9.3e-32 41.2% identity in 257 aa overlap and TR:CAB55664 (EMBL:AL117385) hypothetical 32.2 KD protein SC5G9.16 (295 aa), fasta scores opt: 521 z-score: 637.0 E(): 4.3e-28 36.5% identity in 288 aa overlap. Contains a putative helix-turn-helix motif situated between residues 47..68 (+2.88 SD). 3 2 15 11 [Reporter]SCO4130 (12O3)_PCR/3342 [Gene]SCO4130/3043 NC_003888 SCD72A.16c, possible membrane protein, len: 420aa; contains a possible membrane-spanning hydrophobic region. 3 2 15 10 [Reporter]SCO3041 (13K3)_PCR/3341 [Gene]SCO3041/3042 NC_003888 SCE34.22, hypothetical protein, len: 263 aa; similar to TR:P96873 (EMBL:Z92771) Mycobacterium tuberculosis hypothetical 23.7 kD protein, 229 aa; fasta scores: opt: 442 z-score: 490.8 E(): 7.1e-20; 40.1% identity in 242 aa overlap 3 2 15 9 [Reporter]SCO0197 (14G3)_PCR/3340 [Gene]SCO0197/3041 NC_003888 SCJ12.09c, hypothetical protein, len: 146 aa. Some similarity to Mycobacterium tuberculosis TR:O53625 (EMBL; AL021428) hypothetical 16.6 KD protein (152 aa), fasta scores opt: 291 z-score: 377.8 E(): 1.1e-13 42.9% identity in 133 aa overlap. Also similar to TR:Q9RI34 (EMBL:AL109989) S. coelicolor SCJ12.26 (137 aa), fasta scores: opt: 524 z-score: 568.0 E(): 2.2e-26 62.2% identity in 135 aa overlap 3 2 15 8 [Reporter]SCO1544 (15C3)_PCR/3339 [Gene]SCO1544/3040 NC_003888 SCL2.34, hypothetical protein, len: 195 aa; similar to TR:AAF10140 (EMBL:AE001914) Deinococcus radiodurans conserved hypothetical protein DR0560, 376 aa; fasta scores: opt: 248 z-score: 296.3 E(): 4.1e-09; 37.6% identity in 149 aa overlap 4 3 9 9 [Reporter]SCO3343 (13P18)_PCR/5728 [Gene]SCO3343/5219 NC_003888 SCE7.10c, unknown, len: 124 aa; similar to SW:YRAI_BACSU hypothetical protein from Bacillus subtilis (144 aa) fasta scores; opt: 252, z-score: 308.2, E(): 7.6e-10, (42.4% identity in 92 aa overlap). 4 3 9 8 [Reporter]SCO2987 (14L18)_PCR/5727 [Gene]SCO2987/5218 NC_003888 SCE50.15, possible regulatory protein, len: 168 aa; similar to SW:HPCR_ECOLI (EMBL:S56952) Escherichia coli homoprotocatechuate degradative operon repressor HpcR, 148 aa; fasta scores: opt: 151 z-score: 195.2 E(): 0.002; 27.9% identity in 136 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 4 3 9 7 [Reporter]SCO1723 (15H18)_PCR/5726 [Gene]SCO1723/5217 NC_003888 SCI11.12c, possible RNA polymerase sigma factor, len: 185 aa; weakly similar to RNA polymerase sigma factors e.g. SW:CARQ_MYXXA (EMBL:X71062), CarQ, Myxococcus xanthus RNA polymerase sigma factor (174 aa), fasta scores; opt: 233 z-score: 295.7 E(): 3.9e-09, 29.3% identity in 167 aa overlap. Similar to many putative sigma factors e.g. TR:O53730 (EMBL:AL021932), SigK, Mycobacterium tuberculosis probable RNA polymerase sigma factor (187 aa) (51.4% identity in 173 aa overlap). Similar to others from S.coelicolor e.g. TR:O54163 (EMBL:AL021411) S. coelicolor possible sigma factor (200 aa) (30.2% identity in 182 aa overlap). Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 4 3 9 6 [Reporter]SCO3658 (16D18)_PCR/5725 [Gene]SCO3658/5216 NC_003888 SCH10.36, probable aspartate aminotransferase, len: 399 aa; similar to many e.g. TR:Q56232 (EMBL:D38459) Thermus aquaticus aspartate aminotransferase (385 aa), fasta scores; opt: 731 z-score: 825.9 E(): 0, 36.5% identity in 392 aa overlap. Note that there are three possible start codons. Similar to many putative aminotransferases from Streptomyces coelicolor e.g. TR:O54170 (EMBL:AL021411) SC7H1.11, putative aminotransferase (382 aa) (36.6% identity in 331 aa overlap). Contains Pfam match to entry PF00155 aminotran_1, Aminotransferases class-I, score 159.80, E-value 4.6e-44 4 3 9 5 [Reporter]SCO3216 (17P14)_PCR/5724 [Gene]SCO3216/5215 NC_003888 SCE8.09, probable integral membrane ATPase, len: 796 aa; member of a large family including SW:Y1B2_MYCTU putative cation-transporting ATPase from Mycobacterium tuberculosis (797 aa) fasta scores; opt: 2741, z-score: 2896.9, E(): 0, (56.8% identity in 791 aa overlap). Contains two Pfam matches to entry PF00122 E1-E2_ATPase, E1-E2 ATPases, score 109.40, E-value 9.7e-30 and score 155.80, E-value 7.3e-43, and Prosite match to PS00154 E1-E2 ATPases phosphorylation site. 4 3 9 4 [Reporter]SCO4988 (18L14)_PCR/5723 [Gene]SCO4988/5214 NC_003888 2SCK36.11, probable carbohydrate kinase, len: 351 aa; similar to TR:Q9K6K5 (EMBL:AP001519) Bacillus halodurans 2-keto-3-deoxygluconate kinase (EC 2.7.1.45) KdgK or BH3724, 317 aa; fasta scores: opt: 578 Z-score: 638.8 bits: 126.5 E(): 5.7e-28; 38.835% identity in 309 aa overlap. Contains Pfam match to entry PF00294 pfkB, pfkB family carbohydrate kinase 4 3 9 3 [Reporter]SCP1.286 (19H14)_PCR/5722 [Gene]SCP1.286/5213 NC_003888 SCP1.286, unknown, len: 84aa; similar to SCP1.225 hypothetical protein (previously sequenced as TR:Q9JN71 (EMBL:AJ276673)) from this plasmid fasta scores: E(): 1.1, 40.7% id in 81 aa. 4 3 9 2 [Reporter]SCO0820 (20P10)_PCR/5721 [Gene]SCO0820/5212 NC_003888 SCF43A.10c, unknown, len: 119 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 3 2 15 7 [Reporter]SCO0048 (15O23)_PCR/3338 [Gene]SCO0048/3039 NC_003888 SCJ4.29c, unknown, len: 127 aa 4 3 8 22 [Reporter]SCO4220 (1P6)_PCR/5719 [Gene]SCO4220/5211 NC_003888 2SCD46.34c, conserved hypothetical protein, len: 139aa; similar to others from actinomycetes eg. TR:CAB89437 (EMBL:AL354048) hypothetical protein from Streptomyces coelicolor (145 aa) fasta scores; opt: 105, z-score: 143.5, E(): 1.6, 37.0% identity in 146 aa overlap. 3 2 15 6 [Reporter]SCO0646 (16K23)_PCR/3337 [Gene]SCO0646/3038 NC_003888 SCF91.06, possible TetR-family transcriptional regulator, len: 194 aa. Similar to many regulators e.g. Propionibacterium freudenreichii TR:P72185( EMBL:U51164) repressor protein (HemR protein) (259 aa), fasta scores opt: 249 z-score: 304.0 E(): 1.5e-09 33.8% identity in 142 aa overlap. Also similar to several putative Streptomyces coelicolor regulators e.g. TR:CAB56137 (EMBL:AL117669) SCF12.11 (206 aa), fasta scores opt: 337 z-score: 409.5 E(): 2e-15 36.7% identity in 196 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 39..60 (+3.20 SD). 4 3 8 20 [Reporter]SCO4779 (3H6)_PCR/5717 [Gene]SCO4779/5210 NC_003888 SCD63.11, pkaJ, serine/threonine protein kinase, len: 548 aa; N-terminal region similar to TR:CAB50939 (EMBL:AL096849) Streptomyces coelicolor putative protein serine/threonine kinase SCI11.13, 550 aa; fasta scores: opt: 1006 z-score: 815.3 E(): 0; 56.9% identity in 297 aa overlap and to TR:AAD47061 (EMBL:AF159501) Myxococcus xanthus Pkn4, 643 aa; fasta scores: opt: 639 z-score: 521.3 E(): 1.4e-21; 43.6% identity in 273 aa overlap and C-terminal similar to TR:Q9WTP4 (EMBL:AB019577) Mus musculus UNC-51-like kinase (ULK) 2, Ulk2, 1037 aa; blastp socres: Sum P(2) = 2.6e-24 Identities = 89/289 (30%), Positives = 144/289 (49%). Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and matches to Prosite entries PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature 3 2 15 5 [Reporter]SCO0900 (17G23)_PCR/3336 [Gene]SCO0900/3037 NC_003888 SCM1.33, possible transmembrane efflux protein, len: 427 aa. Similar to several other membrane transport proteins including: Francisella tularensis TR:AAC12938(EMBL:AF055345) tetracycline-resistance protein (396 aa), fasta scores opt: 230 z-score: 259.1 E(): 4.4e-07 25.4% identity in 397 aa overlap and Streptomyces flavopersicus TR:AAD45543(EMBL:U70376) putative spectinomycin export protein, SpcT (433 aa), fasta scores opt: 467 z-score: 520.7 E(): 1.2e-21 30.7% identity in 394 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 3 2 15 4 [Reporter]SCO0028 (18C23)_PCR/3335 [Gene]SCO0028/3036 NC_003888 SCJ4.09c, doubtful CDS, function unknown, len: 47 aa 3 2 15 3 [Reporter]SCO4993 (19O19)_PCR/3334 [Gene]SCO4993/3035 NC_003888 2SCK36.16, possible metal ion transport protein, len: 456 aa; similar to SW:MNTH_SALTY (EMBL:AF161317) Salmonella typhimurium manganese transport protein MntH, 413 aa; fasta scores: opt: 241 Z-score: 273.7 bits: 59.8 E(): 1.2e-07; 20.824% identity in 437 aa overlap. Contains possible hydrophobic membrane spanning regions 3 2 15 2 [Reporter]SCO0979 (20G19)_PCR/3333 [Gene]SCO0979/3034 NC_003888 SCBAC19F3.06c, doubtful CDS, len: 78aa: no significant database matches. 3 2 14 22 [Reporter]SCO1003 (1G15)_PCR/3331 [Gene]SCO1003/3033 NC_003888 2SCG2.16, probable tetR-family transcriptional regulator, len: 194 aa; similar to TR:Q9RL21 (EMBL:AL117385) Streptomyces coelicolor putative transcriptional regulator SC5G9.05, 193 aa; fasta scores: opt: 408 z-score: 498.8 E(): 2.9e-20; 39.4% identity in 193 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Also contains possible helix-turn-helix motif at residues 37..58 (+4.68 SD) 3 2 14 21 [Reporter]SCO4752 (2C15)_PCR/3330 [Gene]SCO4752/3032 NC_003888 SC6G4.30, gcp, probable O-sialoglycoprotein endopept idase, len: 374 aa; similar to many e.g. GCP_PASHA Pasteure lla haemolytica O-sialoglycoprotein endopeptidase (EC 3.4.2 4.57) (325 aa), fasta scores; opt: 871 z-score: 526.3 E(): 4.7e-22, 46.5% identity in 316 aa overlap 3 2 14 20 [Reporter]SCO5920 (3O11)_PCR/3329 [Gene]SCO5920/3031 NC_003888 SC10A5.25c, probable DEAD-box RNA helicase, len: 498 aa; similar to many e.g. RHLE_ECOLI P25888 putative ATP-dependent RNA helicase RhlE (454 aa), fasta scores; opt: 845 z -score: 770.8 E(): 0, 36.2% identity in 434 aa overlap. Con tains possible DEAD-box at aa 221-224 (1 mismatch to prosit e consensus), 2x PS00017 ATP/GTP-binding site motif A (P-lo op) and PS00135 Serine proteases, trypsin family, serine ac tive site, though the last may be spurious. Also contains P fam match to entry helicase_C PF00271, Helicases conserved C-terminal domain, score 108.77 and Pfam match to entry DEA D PF00270, DEAD and DEAH box helicases, score 236.10 3 2 14 18 [Reporter]SCO5455 (5G11)_PCR/3327 [Gene]SCO5455/3030 NC_003888 SC3D11.12, possible two-component system response regulator, len: 224 aa; similar to many proposed response regulators e.g. TRNEW:CAA74720 (EMBL:Y14336) response regulatory protein SenR from Streptomyces reticuli (217 aa) fasta scores; opt: 643, z-score: 725.3, E(): 5.5e-33, 53.8% identity in 208 aa overlap and SW:X13360 (NARL_ECOLI) nitrate/nitrite response regulator protein NarL from Escherichia coli (216 aa) fasta scores; opt: 512, z-score: 580.2, E(): 6.6e-25, 38.6% identity in 207 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain, Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, Prosite match to PS00622 Bacterial regulatory proteins, luxR family signature and helix-turn-helix motif Score 1439 (+4.09 SD) in ID at residue 178. 4 3 8 19 [Reporter]SCO6042 (4D6)_PCR/5716 [Gene]SCO6042/5209 NC_003888 SC1B5.02, unknown, len: 243 aa; similar to hypothetical protein downstream of M. tuberculosis HemY; TR:P71973 (EMBL:Z80225) MTCY441.45C (296 aa), fasta scores; opt: 917 z-score: 1481.6 E(): 0, 59.1% identity in 237 aa overlap 4 3 8 18 [Reporter]SCO5884 (5P2)_PCR/5715 [Gene]SCO5884/5208 NC_003888 SC3F7.04c, unknown, len: 296 aa; some similarity to Archaeoglobus fulgidus hypothetical protein TR:G2648734 (EMBL:AE000977) AF1816 (300 aa), fasta scores; opt: 314 z-score: 322.6 E(): 8.9e-11, 30.2% identity in 301 aa overlap 4 3 8 17 [Reporter]SCO6654 (6L2)_PCR/5714 [Gene]SCO6654/5207 NC_003888 SC5A7.04c, unknown, len: 273 aa; some similarity to hypothetical proteins and to Pseudomonas TR:Q52548 (EMBL:D45424) creatinine amidohydrolase (259 aa), fasta sores; opt: 193 z-score: 273.1 E(): 5.6e-08 27.3% identity in 238 aa overlap. Note 63 aa overlap with convergent downstream ORF 4 3 8 16 [Reporter]SCO0309 (7H2)_PCR/5713 [Gene]SCO0309/5206 NC_003888 SC5G9.18c, probable amide hydrolase, len: 362 aa; similar to 6-aminohexanoate-dimer hydrolases which degrade N-(6-aminohexanoyl)-6-aminohexanoate e.g. TR:P75007 (EMBL:K02336), NylB', Flavobacterium sp. (strain K172) 6-aminohexanoic acid linear oligomer hydrolase (EII') (392 aa), fasta scores; opt: 531 z-score: 598.0 E(): 6.1e-26, 34.1% identity in 323 aa overlap 4 3 8 15 [Reporter]SCO2151 (8D2)_PCR/5712 [Gene]SCO2151/5205 NC_003888 SC6G10.24c, cox3, cytochrome c oxidase subunit III, len: 206aa; similar to many eg. SW:COX3_BACP3 caaC, cytochrome c oxidase subunit III from Bacillus PS3 (207 aa) fasta scores; opt: 397, z-score: 511.9, E(): 3.3e-21, (35.9% identity in 181 aa overlap). Contains Pfam match to entry PF00510 COX3, Cytochrome c oxidase subunit III. 4 3 8 13 [Reporter]SCO1493 (9L22)_PCR/5710 [Gene]SCO1493/5204 NC_003888 SC9C5.17c, possible ATP/GTP binding protein (putative secreted protein), len:295 aa; similar to TR:Q9S4Q8 (EMBL:AF121009) Mycobacterium tuberculosis hypothetical 33.5 kD protein, 342 aa; fasta scores: opt: 307 z-score: 233.4 E(): 1.6e-05; 41.1% identity in 124 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Contains also degenerate 3x repeat: PAPAP(G/Q)PH at N-terminal domain and high content in proline amino acid residues. Contains possible N-terminal region signal peptide sequence 4 3 8 12 [Reporter]SCO2719 (10H22)_PCR/5709 [Gene]SCO2719/5203 NC_003888 SCC46.04, putative secreted protein, len: 147 aa; similar to S. coelicolor unknwon protein SCC46.03c, 131 aa; fasta scores: opt: 626 z-score: 650.1 E(): 9.3e-31; 69.2% identity in 130 aa overlap. Contains possible N-terminal region signal peptide sequence 4 3 8 11 [Reporter]SCO4085 (11D22)_PCR/5708 [Gene]SCO4085/5202 NC_003888 SCD25.21, lipoprotein, len: 202 aa. Contains a possible N-terminal signal sequence and a Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 3 2 14 17 [Reporter]SCO5797 (6C11)_PCR/3326 [Gene]SCO5797/3029 NC_003888 SC4H2.18c, probable secreted protein, len: 405 aa; Contains probable N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Highly similar to upstream CDS SC4H2.19c (409 aa), fasta scores; E(): 0, 61.8% identity in 406 aa overlap 4 3 8 10 [Reporter]SCO2924 (12P18)_PCR/5707 [Gene]SCO2924/5201 NC_003888 SCE19A.24, possible regulator, len: 142 aa; similar to TR:P95753 (EMBL:D50051), SsgA, Streptomyces griseus gene involved in regulation of sporulation and cell division (136 aa), fasta scores; opt: 291 z-score: 368.2 E(): 3.6e-13, 37.3% identity in 126 aa overlap and to SC5F2A.05C (EMBL:AL049587) S. coelicolor putative regulator (138 aa) (38.5% identity in 135 aa overlap) 3 2 14 16 [Reporter]SCO6764 (7O7)_PCR/3325 [Gene]SCO6764/3028 NC_003888 SC6A5.13, probable squalene-hopene cyclase, len: 680 aa; similar to many e.g. SW:SQHC_ALIAC squalene-hopene cyclase A key enzyme in triterpenoid metabolism in Bacillus acidocaldarius (630 aa) fasta scores; opt: 2153, z-score: 2386.3, E(): 0, (51.8% identity in 620 aa overlap). Contains two Pfam matches to entry PF00432 prenyltrans, Prenyltransferase and squalene oxidase repeats and a Prosite match to PS01074 Terpene synthases signature. 4 3 8 9 [Reporter]SCO5014 (13L18)_PCR/5706 [Gene]SCO5014/5200 NC_003888 SCK15.16, possible secreted protein, len: 192 aa. Contains possible N-terminal region signal peptide sequence 3 2 14 15 [Reporter]SCO2474 (8K7)_PCR/3324 [Gene]SCO2474/3027 NC_003888 SC7A8.13, probable metalloproteinase (putative secreted protein), len: 547 aa; similar to TR:O06694 (EMBL:U50548) Vibrio vulnificus metalloprotease, 606 aa; fasta scores: opt: 493 z-score: 516.9 E(): 2.1e-21; 31.5% identity in 461 aa overlap and to SW:PROA_LEGPN (EMBL:M31884) Legionella pneumophila zinc metalloproteinase precursor (EC 3.4.24.-) ProA, 543 aa; fasta scores: opt: 475 z-score: 498.8 E(): 2.1e-20; 31.4% identity in 417 aa overlap. Contains Pfam match to entry PF01447 Peptidase_M4, Thermolysin family peptidase and match to Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Contains also possible N-terminal region signal peptide sequence 3 2 14 14 [Reporter]SCO7743 (9G7)_PCR/3323 [Gene]SCO7743/3026 NC_003888 SC8D11.34c, unknown, len: 161 aa 3 2 14 13 [Reporter]SCO6215 (10C7)_PCR/3322 [Gene]SCO6215/3025 NC_003888 SC9G1.05, unknown, len: 41 aa 3 2 14 12 [Reporter]SCO2369 (11O3)_PCR/3321 [Gene]SCO2369/3024 NC_003888 SCC8A.27c, possible thiol-specific antioxidant protein, len: 152 aa; similar to many e.g. SW:O24364 (BAS1_SPIOL) 2-cys peroxiredoxin Bas1 precursor from Spinacia oleracea (Spinach) (265 aa) fasta scores; opt: 352, z-score: 450.7, E(): 1.2e-17, 39.9% identity in 158 aa overlap and SW:Q10520 (Y01J_MYCTU) hypothetical protein from Mycobacterium tuberculosis (153 aa) fasta scores; opt: 507, z-score: 648.2, E(): 1.2e-28, 48.7% identity in 150 aa overlap. Contains Pfam match to entry PF00578 AhpC-TSA, AhpC/TSA family. 3 2 14 11 [Reporter]SCO4126 (12K3)_PCR/3320 [Gene]SCO4126/3023 NC_003888 SCD72A.12c, possible membrane protein, len: 495aa; similar in regions to others from actinomycetes eg. TR:Q9X8A2 (EMBL:AL049645) hypothetical protein from Streptomyces coelicolor (427 aa) fasta scores; opt: 266, z-score: 260.7, E(): 4.7e-07, 27.7% identity in 296 aa overlap. Contains possible membrane-spanning hydrophobic region. 3 2 14 10 [Reporter]SCO3194 (13G3)_PCR/3319 [Gene]SCO3194/3022 NC_003888 SCE22.11, possible lipoprotein, len: 407 aa; similar to TR:CAB76012 (EMBL:AL157916) Streptomyces coelicolor putative lipoprotein SC3D11.15, 400 aa; fasta scores: opt: 1416 z-score: 1518.4 E(): 0; 52.4% identity in 403 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal domain signal peptide sequence 3 2 14 9 [Reporter]SCO0635 (14C3)_PCR/3318 [Gene]SCO0635/3021 NC_003888 SCF56.19, hypothetical protein, len: 297 aa; similar to TR:Q9XAK3 (EMBL:AL079348) Streptomyces coelicolor hypothetical 33.9 kD protein SC66T3.10c, 318 aa; fasta scores: opt: 133 z-score: 153.6 E(): 0.36; 31.6% identity in 212 aa overlap 3 2 14 8 [Reporter]SCO1597 (14O23)_PCR/3317 [Gene]SCO1597/3020 NC_003888 SCI35.19c, probable rRNA methylase, len: 282 aa; similar to many members of ther spoU famil e.g. TSNR_STRLU 23s rRNA methyltransferase (270 aa), fasta scores; opt: 254 z-score: 251.7 E(): 9e-07, 27.0% identity in 270 aa overlap. Contains Pfam match to entry PF00588 SpoU_methylase, SpoU rRNA Methylase family, score 107.90, E-value 1.9e-28 3 2 14 7 [Reporter]SCO0195 (15K23)_PCR/3316 [Gene]SCO0195/3019 NC_003888 SCJ12.07, possible lipoprotein, len: 228 aa. Contains an N-terminal signal sequence and a PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 3 2 14 6 [Reporter]SCO1628 (16G23)_PCR/3315 [Gene]SCO1628/3018 NC_003888 SCI41.11c, cvnC9, hypothetical protein, len: 138 aa. Similar to TR:CAB41560 (EMBL:AL049727) Streptomyces coelicolor SC9B1.14C hypothetical 12.8 kd protein, 119 aa; fasta scores: opt: 240 z-score: 262.1 E(): 3.1e-07; 45.0% identity in 109 aa overlap and to TR:Q9AJP2 (EMBL:AB044803) Streptomyces griseus ORF3, 123 aa; fasta scores: opt: 472 Z-score: 497.8 E(): 4.3e-20; 58.273% identity in 139 aa overlap 3 2 14 5 [Reporter]SCO1763 (17C23)_PCR/3314 [Gene]SCO1763/3017 NC_003888 SCI51.03, possible iron-sulphur protein (putative secreted protein), len: 131 aa; similar to part of many cytochrome B6-F complex iron-sulfur subunits from bacteria and chloroplasts e.g. SW:UCRI_CHLLT (EMBL:X73628), PetC, Chlorobium limicola F. sp. thiosulfatophilum iron-sulfur subunit (180 aa), fasta scores; opt: 198 z-score: 217.9 E(): 8.2e-05, 34.4% identity in 90 aa overlap. Similar to TR:Q9Z510 (EMBL:AL035591) Streptomyces coelicolor putative iron sulphur binding protein (141 aa) (36.6% identity in 131 aa overlap). Contains Pfam match to entry PF00355 Rieske, Rieske iron-sulfur protein. Contains possible N-terminal region signal peptide sequence 3 2 14 4 [Reporter]SCP1.20c (18O19)_PCR/3313 [Gene]SCP1.20c/3016 NC_003888 SCP1.20c, unknown, len: 260aa; 3 2 14 3 [Reporter]SCP1.306 (19K19)_PCR/3312 [Gene]SCP1.306/3015 NC_003888 SCP1.306, unknown, len: 218aa; N-terminal region weakly similar to many eg. SW:P71311 (YAIS_ECOLI) hypothetical protein from Escherichia coli (185 aa) fasta scores; opt: 221, z-score: 290.7, E(): 1e-08, 28.7% identity in 164 aa overlap. 3 2 14 2 [Reporter]SCO5983 (20C19)_PCR/3311 [Gene]SCO5983/3014 NC_003888 SCBAC16H6.18c, possible fatty acid-CoA ligase, len: 563 aa: similar to many e.g. TR:Q53005 (EMBL:U02033) 4-hydroxybenzoate-coenzyme A ligase rom Rhodopseudomonas palustris (539 aa) fasta scores; opt: 758, Z-score: 808.8, 33.747% identity (35.667% ungapped) in 483 aa overlap and TR:BAB48174 (EMBL:AP002995) probable acid-CoA ligase from Rhizobium loti (541 aa) fasta scores; opt: 1793, Z-score: 1909.7, 51.866% identity (53.565% ungapped) in 536 aa overlap. Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and Prosite match to PS00455 Putative AMP-binding domain signature. 3 2 13 22 [Reporter]SCO0995 (1C15)_PCR/3309 [Gene]SCO0995/3013 NC_003888 2SCG2.08, possible methyltransferase, len: 249 aa; similar to TR:P72459 (EMBL:Y08763) Streptomyces griseus methyltransferase StsG, 253 aa; fasta scores: opt: 337 z-score: 418.3 E(): 8.9e-16; 28.9% identity in 242 aa overlap. Contains Pfam match to entry PF01209 Ubie_methyltran, ubiE/COQ5 methyltransferase family 3 2 13 21 [Reporter]SCO2777 (2O11)_PCR/3308 [Gene]SCO2777/3012 NC_003888 SCC105.08, accC, acetyl/propionyl CoA carboxylase alpha subunit, len: 646 aa; similar to SW:PCCA_HUMAN (EMBL:X14608) Homo sapiens propionyl-CoA carboxylase alpha chain precursor (EC 6.4.1.3) PccA, 703 aa; fasta scores: opt: 1539 z-score: 1630.1 E(): 0; 43.0% identity in 665 aa overlap and to SW:ACCC_ECOLI (EMBL:M79446) Escherichia coli biotin carboxylase (EC 6.3.4.14), alpha subunit of acetyl CoA carboxylase (EC 6.4.1.2) AccC or FabG, 449 aa; fasta scores: opt: 1439 z-score: 1527.1 E(): 0; 47.9% identity in 451 aa overlap and to N-terminal region of TR:CAB59603 (EMBL:AL132662) Streptomyces coelicolor pyruvate carboxylase SCF11.26c, 1124 aa; fasta scores: opt: 1367 z-score: 1204.5 E(): 0; 44.3% identity in 569 aa overlap. Contains Pfam matches to entries PF00289 CPSase_L_chain, Carbamoyl-phosphate synthase (CPSase) and Pfam match to entry PF00364 biotin_lipoyl, Biotin-requiring enzymes and matches to Prosite entries PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00188 Biotin-requiring enzymes attachment site 3 2 13 20 [Reporter]SCO5914 (3K11)_PCR/3307 [Gene]SCO5914/3011 NC_003888 SC10A5.19c, probable transcriptional regulator, len: 330 aa; similar to many membres of the LysR family e.g. MPR R_STRCO P43160 small neutral protease regulatory protein (316 aa), fasta cores; opt: 510 z-score: 581.6 E(): 3.4e-25, 35.8% identity in 324 aa overlap. Contains PS00044 Bacterial regulatory proteins, lysR family signature 3 2 13 19 [Reporter]SCO6021 (4G11)_PCR/3306 [Gene]SCO6021/3010 NC_003888 SC1C3.09, unknown, len: 541 aa; some similarity to hypothetical proteins e.g. M. tuberculosis TR:O06628 (EMBL:Z95618) MTCY07H7A.03 (532 aa), fasta scores; opt: 897 z-score: 779.8 E(): 0, 35.4% identity in 525 aa overlap. Also similar to two genes linked to capsular polysaccharide biosynthesis genes in Neisseria meningitidis: TR:Q51151 (EMBL:L09188) (373 aa), fasta scores; opt: 547 z-score: 579.0 E(): 5.1e-25, 29.3% identity in 355 aa overlap and TR:G2979674 (EMBL:AF019760) SACB (545 aa), fasta scores; opt: opt: 594 z-score: 550.3 E(): 2e-23, 31.4% identity in 322 aa overlap. Also smilar to downstream genes SC1C3.11 (E(): 0, 47.7% identity in 509 aa overlap) and SC1C3.10 (E(): 1.6e-16, 28.0% identity in 542 aa overlap) 4 4 6 18 [Reporter]SCO6333 (5H1)_PCR/7686 [Gene]SCO6333/6999 NC_003888 SC3A7.01c, unknown, partial CDS, len: >68 aa,SC10H5.09c, probable integral membrane protein, partial CDS len: >143 aa; some similarity in C-terminus to S. coelicolor hypothetical protein TR:O54106 (EMBL:AL021529) SC10A5.15 (114 aa), fasta scores; opt: 145 z-score: 233.8 E(): 9.2e-06, 33.3% identity in 81 aa overlap. Overlaps and extends SC3A7.01c 4 4 6 17 [Reporter]SCO7331 (6D1)_PCR/7685 [Gene]SCO7331/6998 NC_003888 SC410.10c, unknown, len: 363 aa. Weakly similar to several proteins of undefined function e.g. Escherichia coli SW:YBDK_ECOLI(EMBL:AE000163) hypothetical 41.7 kd protein (372 aa), fasta scores opt: 369 z-score: 434.5 E(): 9.6e-17 26.8% identity in 355 aa overlap. 4 4 6 16 [Reporter]SCO7319 (6P21)_PCR/7684 [Gene]SCO7319/6997 NC_003888 SC5F8.29c, possible oxidoreductase, len: 481 aa. Similar to many dehydrogenases including: Escherichia coli SW:DHNA_ECOLI(EMBL:V00306) NADH dehydrogenase (EC 1.6.99.3) (433 aa), fasta scores opt: 438 z-score: 509.8 E(): 6.4e-21 26.3% identity in 384 aa overlap and Streptomyces coelicolor TR:Q9ZC17(EMBL:AL033505) putative dehydrogenase, SC1E6.05 (629 aa), fasta scores opt: 1148 z-score: 1329.8 E(): 0 44.3% identity in 420 aa overlap. Contains a Pfam match to entry PF00070 pyr_redox, Pyridine nucleotide-disulphide oxidoreductase. Note codon 27 may offer an alternative translational start site. 4 4 6 15 [Reporter]SCO6955 (7L21)_PCR/7683 [Gene]SCO6955/6996 NC_003888 SC6F7.08, possible membrane protein, len: 254 aa. The N-terminal hydrophobic domain of this protein is highly similar to several e.g. Bacillus firmus TR:AAF21814(EMBL:AF097740) multiple resistance and pH regulation related protein C MrpC (112 aa), fasta scores opt: 260 z-score: 272.3 E(): 1e-07 42.1% identity in 107 aa overlap. Contains 3 N-terminal membrane spanning hydrophobic domains. Contains a Pfam match to entry PF01898 DUF67, Protein of unknown function. 4 4 6 14 [Reporter]SCO6892 (8H21)_PCR/7682 [Gene]SCO6892/6995 NC_003888 SC7F9.44, unknown, len: 160 aa. 4 4 6 13 [Reporter]SCO7170 (9D21)_PCR/7681 [Gene]SCO7170/6994 NC_003888 SC9A4.32, possible secreted sugar hydrolase, len: 786 aa; C-terminal region similar to TR:O88021 (EMBL:AL031107) Streptomyces coelicolor putative secreted glucosidase SC5A7.15c, 466 aa; fasta scores: opt: 270 z-score: 286.2 E(): 1.8e-08; 27.2% identity in 427 aa overlap. Contains possible N-terminal region signal peptide sequence 3 2 13 18 [Reporter]SCO5451 (5C11)_PCR/3305 [Gene]SCO5451/3009 NC_003888 SC3D11.08c, probable ABC transporter, len: 660aa; similar to many eg. TRNEW:CAB66302 (EMBL:AL136519) ABC transporter protein, ATP-binding component from Streptomyces coelicolor (1243 aa) fasta scores; opt: 682, z-score: 703.6, E(): 8.8e-32, 34.4% identity in 608 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, Pfam match to entry PF00664 ABC_membrane, ABC transporter transmembrane region, Prosite match to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 4 4 6 12 [Reporter]SCO1956 (10P17)_PCR/7680 [Gene]SCO1956/6993 NC_003888 SCC54.16, probable transcriptional regulator (lacI family), len: 368 aa; similar to many lacI family transcriptional regulators e.g. SW:RBSR_ECOLI RbsR, ribose operon repressor from Escherichia coli (329 aa) fasta scores; opt: 611, z-score: 696.2, E(): 1.8e-31, (34.2% identity in 336 aa overlap). Contains PS00356 Bacterial regulatory proteins, lacI family signature and Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family, score 102.80, E-value 6.7e-27. Also contains Pfam match to entry PF00356 lacI, Bacterial regulatory proteins, lacI family, score 36.60, E-value 7.4e-09 which corresponds to a probable helix-turn-helix at 3-24aa (+5.62 SD). 3 2 13 17 [Reporter]SCO7444 (6O7)_PCR/3304 [Gene]SCO7444/3008 NC_003888 SC5C11.01c, possible cytochrome P450, partial CDS, len:> 40 aa. This CDS forms the N-terminus of SC6D11.40c, carried on the adjoining cosmid, which is weakly similar to many cytochrome P450's. This similarity is lost in this portion of the predicted protein.,SC6D11.40c, possible cytochrome P450, partial CDS, len:> 421 aa. Weakly similar to many cytochrome P450's including: Saccharopolyspora erythraea (Streptomyces erythraeus) SW:CPXQ_SACER(EMBL:U82823) cytochrome P450 113A1 (erythromycin B/D C-12 hydroxylase) (397 aa), opt: 259 z-score: 300.0 E(): 2.7e-09 30.5% identity in 361 aa overlap. Overlaps and extends into CDS SC5C11.01c on the adjoining cosmid. Contains a Prosite hit to PS00086 Cytochrome P450 cysteine heme-iron ligand signature and a Pfam match to entry PF00067 p450, Cytochrome P450. 4 4 6 11 [Reporter]SCO4524 (11L17)_PCR/7679 [Gene]SCO4524/6992 NC_003888 SCD35.31, putative membrane protein, len: 239 aa. Contains possible hydrophobic membrane spanning region 3 2 13 16 [Reporter]SCO6760 (7K7)_PCR/3303 [Gene]SCO6760/3007 NC_003888 SC6A5.09, probable phytoene synthase, len: 312aa; similar to many egs. SW:CRTB_MYCTU probable phytoene synthase from Mycobacterium tuberculosis (302 aa) fasta scores; opt: 791, z-score: 925.6, E(): 0, (48.3% identity in 286 aa overlap) and SW:PSY_ARATH phytoene synthase precusor from Arabidopsis thaliana (mouse ear cress) (423 aa) fasta scores; opt: 493, z-score: 577.3, E(): 7.4e-25, (34.4% identity in 282 aa overlap). Contains Pfam match to entry PF00494 SQS_PSY, Squalene and phytoene synthases, score 168.70, E-value 9.4e-47 and Prosite matches to PS01044 Squalene and phytoene synthases signature 1 and PS01045 Squalene and phytoene synthases signature 2. 4 4 6 9 [Reporter]SCO5525 (13D17)_PCR/7677 [Gene]SCO5525/6991 NC_003888 SC1C2.06, ureAB, probable fusion of urease beta and gamma subunits, len: 227 aa; N-terminus is similar to UreA e.g. URE3_ECOLI urease gamma subunit (EC 3.5.1.5) (100 aa), fasta scores; opt: 300 z-score: 398.9 E(): 5.7e-15, 44.4% identity in 99 aa overlap; C-terminus is similar to ureB e.g. URE2_YEREN urease beta subunit (EC 3.5.1.5) (163 aa), fasta scores; opt: 303 z-score: 390.6 E(): 1.6e-14, 40.9% identity in 132 aa overlap. This fusion also exists in Helicobacters, where it is called the urease alpha subunit; URE1_HELFE urease alpha subunit (EC 3.5.1.5) (237 aa), fasta scores; opt: 529 z-score: 603.4 E(): 2.3e-26, 42.0% identity in 226 aa overlap. Contains Pfam matches to entry PF00547 urease_gamma, Urease, gamma subunit, score 116.70, E-value 7.9e-35 and to entry PF00699 Urease_beta, Urease beta subunit, score 91.80, E-value 1.4e-23 3 2 13 14 [Reporter]SCO7738 (9C7)_PCR/3301 [Gene]SCO7738/3006 NC_003888 SC8D11.29c, conserved hypothetical protein, len: 127 aa; similar to SW:Y854_PYRHO (EMBL:AP000003) Pyrococcus horikoshii hypothetical protein PH854, 137 aa; fasta scores: opt: 338 z-score: 424.9 E(): 3.9e-16; 45.1% identity in 122 aa overlap. Contains Pfam match to entry PF01042 UPF0076, Domain of unknown function UPF0076 4 4 6 8 [Reporter]SCO3777 (14P13)_PCR/7676 [Gene]SCO3777/6990 NC_003888 SCH63.24, probable peptidase, len: 375 aa; similar to SW:PEPQ_LACDL (EMBL:Z34896) Lactobacillus delbrueckii XAA-PRO dipeptidase (EC 3.4.13.9) PepQ, 368 aa; fasta scores: opt: 623 z-score: 715.2 E(): 2.6e-32; 32.1% identity in 365 aa overlap and to TR:CAB41569 (EMBL:AL049727) Streptomyces coelicolor putative dipeptidase SC9B1.23, 376 aa; fasta scores: opt: 1608 z-score: 1515.1 E(): 0; 63.5% identity in 373 aa overlap. Contains Pfam match to entry PF00557 Peptidase_M24, metallopeptidase family M24 3 2 13 13 [Reporter]SCO6266 (10O3)_PCR/3300 [Gene]SCO6266/3005 NC_003888 SCAH10.31, scbA, len: 314 aa; identical to previously sequenced TR:O86851 (EMBL:AJ007731) Streptomyces coelicolor ScbA protein, 314 aa and to SW:AFSA_STRGR (EMBL:M24250) Streptomyces griseus possible A-factor biosynthesis enzyme AfsA, 301 aa; fasta scores: opt: 1345 z-score: 1570.4 E(): 0; 66.1% identity in 292 aa overlap 3 2 13 12 [Reporter]SCO2363 (11K3)_PCR/3299 [Gene]SCO2363/3004 NC_003888 SCC8A.21c, possible ATP/GTP-binding protein, len: 388aa; similar to TR:AAF11768 (EMBL:AE002054) hypothetical protein from Deinococcus radiodurans (310 aa) fasta scores; opt: 146, z-score: 175.2, E(): 0.027, 32.0% identity in 347 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 2 13 11 [Reporter]SCO4781 (12G3)_PCR/3298 [Gene]SCO4781/3003 NC_003888 SCD63.13, possible cholesterol oxidase, len: 602 aa; similar to TR:Q59530 (EMBL:U00015) Mycobacterium leprae probable cholesterol oxidase precursor (EC 1.1.3.6) ChoD, 585 aa; fasta scores: opt: 1114 z-score: 1181.0 E(): 0; 60.5% identity in 583 aa overlapand to SW:CHOD_BREST (EMBL:D00712) Brevibacterium sterolicum cholesterol oxidase precursor (EC 1.1.3.6) ChoD, 552 aa; fasta scores: opt: 228 z-score: 244.5 E(): 3.4e-06; 26.9% identity in 576 aa overlap 3 2 13 10 [Reporter]SCO3189 (13C3)_PCR/3297 [Gene]SCO3189/3002 NC_003888 SCE22.06, possible membrane protein, len: 159 aa. Contains possible hydrophobic membrane spanning region and high content in alanine amino acid residues 3 2 13 9 [Reporter]SCO3477 (13O23)_PCR/3296 [Gene]SCO3477/3001 NC_003888 SCE65.13c, possible dehydrogenase, len: 344 aa; similar to TR:CAB50292 (EMBL:AJ248287) Pyrococcus abyssi threonine 3-dehydrogenase (EC 1.1.1.103) 348 aa; fasta scores: opt: 567 z-score: 616.8 E(): 5.6e-27; 33.8% identity in 328 aa overlap and to SW:TDH_BACSU (EMBL:Z99112) threonine 3-dehydrogenase (EC 1.1.103) Tdh, 347 aa; fasta scores: opt: 544 z-score: 592.1 E(): 1.3e-25; 31.4% identity in 331 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases 3 2 13 8 [Reporter]SCO1699 (14K23)_PCR/3295 [Gene]SCO1699/3000 NC_003888 SCI30A.20c, possible transcriptional regulator, len: 215 aa; similar to many e.g. TR:CAB39717 (EMBL:AL049485) putative regulatory protein from Streptomyces coelicolor (223 aa) fasta scores; opt: 330, z-score: 413.5, E(): 1.1e-15, (33.8% identity in 216 aa overlap) and TR:AAD13556 (EMBL:AF080235) LanK regulator of landomycin biosynthesis in Streptomyces cyanogenus S136 (192 aa) fasta scores; opt: 170, z-score: 218.6, E(): 7.6e-05, (31.8% identity in 154 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Contains possible helix-turn-helix motif (+4.32 SD) 43-64aa. 4 4 6 7 [Reporter]SCO3727 (15L13)_PCR/7675 [Gene]SCO3727/6989 NC_003888 SCH22A.05, possible stress response protein, len: 194 aa; similar to TR:AAF11772 (EMBL:AE002055) Deinococcus radiodurans tellurium resistance protein TerZ, 195 aa; fasta scores: opt: 300 z-score: 364.6 E(): 6.8e-13; 31.5% identity in 178 aa overlap and to SW:TERZ_SERMA (EMBL:L38824) Serratia marcescens tellurium resistance protein TerZ, 193 aa; fasta scores: opt: 263 z-score: 321.2 E(): 1.8e-10; 28.2% identity in 195 aa overlap 4 4 6 6 [Reporter]SCO0039 (16H13)_PCR/7674 [Gene]SCO0039/6988 NC_003888 SCJ4.20, unknown, len: 205 aa; similar to the N-terminal region of TR:O53411 (EMBL:AL021897) hypothetical protein from Mycobacterium tuberculosis (360 aa) fasta scores; opt: 165, z-score: 190.9, E(): 0.0028, (34.1% identity in 135 aa overlap). 4 4 6 5 [Reporter]SCO0970 (17D13)_PCR/7673 [Gene]SCO0970/6987 NC_003888 SCM11.25c, probable dehydrogenase/reductase (putative secreted protein), len: 244 aa; similar to TR:CAB52925 (EMBL:AL109949) Streptomyces coelicolor putative oxidoreductase SCJ11.40, 250 aa; fasta scores: opt: 526 z-score: 621.9 E(): 2.9e-27; 42.1% identity in 242 aa overlap and to SW:DHG3_BACME (EMBL:D10625) Bacillus megaterium glucose 1-dehydrogenase III (EC 1.1.1.47) GdhIII, 261 aa; fasta scores: opt: 430 z-score: 509.8 E(): 5.1e-21; 36.4% identity in 247 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus. Contains possible N-terminal region signal peptide sequence 4 4 6 4 [Reporter]SCO0408 (18P9)_PCR/7672 [Gene]SCO0408/6986 NC_003888 SCF51.07, probable methyltransferase, len: 262 aa; some similarity to TR:Q9ZGH6 (EMBL:AF079762) Streptomyces venezuelae N,N-dimethyltransferase DesVI 273 aa; fasta scores: opt: 165 z-score: 205.9 E(): 0.00043; 28.3% identity in 237 aa overlap and to TR:CAB49808 (EMBL:AJ248285) Pyrococcus abyssi menaquinone biosynthesis methyltransferase, 248 aa; fasta scores: opt: 165 z-score: 205.6 E(): 0.00044; 27.3% identity in 183 aa overlap 4 4 6 3 [Reporter]SCP1.91c (19L9)_PCR/7671 [Gene]SCP1.91c/6985 NC_003888 SCP1.91c, possible GntR-family transcriptional regulator, len: 252aa; similar to many from extrachromosomal genetic elements eg. TR:Q9RIQ2 (EMBL:AJ243257) KorA protein from plasmid pSNA1 of Streptomyces natalensis (245 aa) fasta scores; opt: 477, z-score: 554.9, E(): 1.9e-23, 37.9% identity in 243 aa overlap and SW:P22405 (KORA_STRLI) KorA protein from plasmid pIJ101 of Streptomyces lividans (241 aa) fasta scores; opt: 393, z-score: 459.0, E(): 4.3e-18, 34.9% identity in 235 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and helix-turn-helix motif (Score 984 (+2.54 SD)) at residue 36-57. 4 4 6 2 [Reporter]SCO4052 (20D9)_PCR/7670 [Gene]SCO4052/6984 NC_003888 2SCD60.18, probable glucose 1-dehydrogenase, len: 264 aa; similar to SW:DHG2_BACME (EMBL:D90044) Bacillus megaterium glucose 1-dehydrogenase II (EC 1.1.1.47) GdhII, 261 aa; fasta scores: opt: 563 z-score: 619.9 E(): 5.7e-27; 38.7% identity in 256 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus 4 4 5 22 [Reporter]SCO5380 (1D5)_PCR/7668 [Gene]SCO5380/6983 NC_003888 2SC6G5.24, possible membrane protein, len: 62aa; weakly similar to TR:Q9XA75 (EMBL:AL096822) putative membrane protein from Streptomyces coelicolor (83 aa) fasta scores; opt:82, z-score: 135.0, E(): 4.7, 37.0% identity in 54 aa overlap. Contains possible membrane-spanning hydrophobic regions. 4 4 5 21 [Reporter]SCO5370 (2P1)_PCR/7667 [Gene]SCO5370/6982 NC_003888 2SC6G5.14, atpH, ATP synthase delta chain (EC 3.6.1.34), len: 274aa; similar to many eg. SW:P00831 (ATPD_ECOLI) ATP synthase delta chain from Escherichia coli (177 aa) fasta scores; opt: 184, z-score: 217.1, E(): 0.00013, 27.0% identity in 141 aa overlap. Almost identical to SW:P50008 (ATPD_STRLI) ATP synthase delta chain from Streptomyces lividans (272 aa) fasta scores; opt: 1515, z-score: 1684.3, E(): 0, 93.0% identity in 271 aa overlap. Contains Pfam match to entry PF00213 OSCP, ATP synthase delta (OSCP) subunit and Prosite match to PS00389 ATP synthase delta (OSCP) subunit signature. 4 4 5 20 [Reporter]SCO5881 (3L1)_PCR/7666 [Gene]SCO5881/6981 NC_003888 SC2E9.22c, redZ, response regulator, partial CDS, len: >67 aa; identical to TR:P72402 (EMBL:Y07902) RedZ gene (217 aa). Contains probable helix-turn-helix motif at aa 12 to 33 (Score 1209, +3.30 SD),SC3F7.01c, redZ, response regulator, partial CDS, len >184 aa; identical to TR:P72402 (EMBL:Y07902) redZ gene (217 aa), and similar to many two-component regulators e.g. UVRY_ECOLI P07027 UvrY protein from Escherichia coli (218 aa), fasta scores; opt: 159 z-score: 291.2 E(): 5e-09, 26.3% identity in 186 aa overlap. Contains TTA leucine codon; a potential target for action of bldA, probable Helix-turn-helix motif at aa 162-183 (Score 1209, +3.30 SD) and Pfam match to entry response_reg PF00072, Response regulator receiver domain, score 31.36 4 4 5 18 [Reporter]SCO7096 (5D1)_PCR/7664 [Gene]SCO7096/6980 NC_003888 SC3A4.22, possible membrane protein, len: 114 aa. Contains a possible membrane spanning hydrophobic domain. 4 4 5 17 [Reporter]SCO7055 (5P21)_PCR/7663 [Gene]SCO7055/6979 NC_003888 SC4G1.21, possible methyltransferase, len: 241 aa; similar to TR:P72459 (EMBL:Y08763) Streptomyces griseus methyltransferase StsG, 253 aa; fasta scores: opt: 207 z-score: 238.8 E(): 7.8e-06; 34.1% identity in 138 aa overlap 4 4 5 16 [Reporter]SCO7314 (6L21)_PCR/7662 [Gene]SCO7314/6978 NC_003888 SC5F8.24, probable RNA polymerase sigma factor, len: 299 aa. Highly similar to many e.g. Streptomyces coelicolor SW:RPOF_STRCO (EMBL:L11648) RNA polymerase sigma-F factor, SigF (287 aa), fasta scores opt: 687 z-score: 772.9 E(): 0 43.6% identity in 275 aa overlap. Contains a Pfam match to entry PF00140 sigma70, Sigma-70 factor and a putative helix-turn-helix motif situated between residues 242..263 (+6.95 SD). 4 4 5 15 [Reporter]SCO7424 (7H21)_PCR/7661 [Gene]SCO7424/6977 NC_003888 SC6D11.20, possible MarR-family transcriptional regulator, len: 155 aa. Weakly similar to Streptomyces coelicolor TR:CAB56677 (EMBL:AL121596) MarR-family protein, SCF51A.25 (148 aa), fasta scores opt: 209 z-score: 254.7 E(): 9.1e-07 33.1% identity in 124 aa overlap. Contains a Pfam match to entry PF01047 MarR, MarR family with the putative helix-turn-helix motif situated between residues 53..74 (+4.29 SD). 4 4 5 14 [Reporter]SCO6888 (8D21)_PCR/7660 [Gene]SCO6888/6976 NC_003888 SC7F9.40, unknown, len: 232 aa. 4 4 5 13 [Reporter]SCO6435 (9P17)_PCR/7659 [Gene]SCO6435/6975 NC_003888 SC9B5.02, unknown, len: 128 aa; similar to SW:WH16_STRCO 16.7kD protein in whiE locus of Streptomyces coelicolor (157 aa) fasta scores; opt: 199, z-score: 252.3, E(): 9.3e-07, (31.2% identity in 125 aa overlap). 4 4 5 12 [Reporter]SCO1952 (10L17)_PCR/7658 [Gene]SCO1952/6974 NC_003888 SCC54.12c, unknown, len: 338aa; similar to many hypothetical proteins e.g. SW:YR39_MYCTU hypothetical protein from Mycobacterium tuberculosis (301 aa) fasta scores; opt: 1071, z-score: 1215.8, E(): 0, (55.6% identity in 284 aa overlap) 4 4 5 11 [Reporter]SCO4361 (11H17)_PCR/7657 [Gene]SCO4361/6973 NC_003888 SCD19.16, possible integral membrane protein, len: 185 aa. Contains possible hydrophobic membrane spanning regions 4 4 5 10 [Reporter]SCO4278 (12D17)_PCR/7656 [Gene]SCO4278/6972 NC_003888 SCD95A.11, hypothetical protein, len: 145 aa; similar to SW:YAEJ_ECOLI (EMBL:L38619) Escherichia coli hypothetical 15.6 kD protein in MesJ-CutF intergenic region (ORF140) YaeJ, 140 aa; fasta scores: opt: 338 z-score: 405.8 E(): 3.8e-15; 45.7% identity in 138 aa overlap. Contains Pfam match to entry PF00472 RF-1, Peptidyl-tRNA hydrolase domain and match to Prosite entry PS00745 Prokaryotic-type class I peptide chain release factors signature 3 3 11 5 [Reporter]SCO4961 (17G18)_PCR/5264 [Gene]SCO4961/4799 NC_003888 2SCK31.21c possible marR-family transcriptional regulator, len: 166 aa; similar to SW:YXAD_BACSU (EMBL:AB005554) Bacillus subtilis hypothetical transcriptional regulator in AsnH-GntR intergenic region YxaD, 143 aa; fasta scores: opt: 268 z-score: 338.9 E(): 2.4e-11; 35.0% identity in 137 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family and possible helix-turn-helix motif at residues 58..79 (+2.70 SD) 4 4 5 9 [Reporter]SCO0055 (13P13)_PCR/7655 [Gene]SCO0055/6971 NC_003888 SCJ4.36c, possible membrane-associated oxidoreductase, len: 503aa; N-terminal region similar to TR:Q53931 (EMBL:X64420) HisD L-histidinol:NAD oxidoreductase from Streptomyces coelicolor (203 aa) fasta scores; opt: 345, z-score: 401.6, E(): 5.2e-15, (35.6% identity in 177 aa overlap). Also similar to TR:O88013 (EMBL:AL031107) hypothetical protein from Streptomyces coelicolor (527 aa) fasta scores; opt: 684, z-score: 783.6, E(): 0, (37.0% identity in 525 aa overlap) 3 3 11 4 [Reporter]SCO0683 (18C18)_PCR/5263 [Gene]SCO0683/4798 NC_003888 SCF15.04c, hypothetical protein, len: 174 aa. Weakly similar to several conserved proteins of undefined function e.g. Methanococcus jannaschii SW:Y714_METJA(EMBL:U67518) hypothetical protein (118 aa), fasta scores opt: 182 z-score: 196.7 E(): 0.0014 30.4% identity in 115 aa overlap. 4 4 5 8 [Reporter]SCO3473 (14L13)_PCR/7654 [Gene]SCO3473/6970 NC_003888 SCE65.09c, probable aldolase, len: 215 aa; similar to SW:ALKH_BACSU (EMBL:L47838) Bacillus subtilis KHG/KDPG aldolase AlkH, 347 aa; fasta scores: opt: 457 z-score: 523.2 E(): 9.1e-22; 39.5% identity in 200 aa overlap and to Streptomyces coelicolor SCE65.31c, 225 aa; fasta scores: opt: 424 z-score: 429.4 E(): 1.5e-18; 37.7% identity in 215 aa overlap. Contains Pfam match to entry PF01081 Aldolase, KDPG and KHG aldolase and match to Prosite entry PS00160 KDPG and KHG aldolases Schiff-base forming residue 3 3 11 3 [Reporter]SCO1363 (19O14)_PCR/5262 [Gene]SCO1363/4797 NC_003888 SC10A9.05c, possible integral membrane protein, len: 334 aa; low similarity to SW:YCBK_BACSU (EMBL:D30808) Bacillus subtilis hypothetical 34.0 kDa protein in GltP-Pcp intergenic region YcbK, 312 aa; fasta scores: opt: 200 z-score: 236.4 E(): 1e-05; 22.6% identity in 292 aa overlap. Contains 2x Pfam matches to entry PF00892 DUF6, Integral membrane protein DUF6. Also contains possible hydrophobic membrane spanning regions 3 3 11 2 [Reporter]SCO3048 (20G14)_PCR/5261 [Gene]SCO3048/4796 NC_003888 SCBAC19G2.03c, possible membrane protein, len: 397aa: similar to TR:O69850 (EMBL:AL023702) putative transcriptional regulator from Streptomyces coelicolor (366 aa) fasta scores; opt: 615, Z-score: 578.6, 34.694% identity (37.072% ungapped) in 343 aa overlap. Contains possible membrane-spanning hydrophobic region. Also has region of similarity with adjacent CDS SCBAC19F3.02. 3 3 10 22 [Reporter]SCO5277 (1G10)_PCR/5259 [Gene]SCO5277/4795 NC_003888 SCCB12.01, possible ion chelatase subunit (fragment), len: >98 aa; similar to SW:BCHI_RHOCA (EMBL:Z11165) Rhodobacter capsulatus magnesium-chelatase 38 Kda subunit (MG-protoporphyrin IX chelatase) BchI, 350 aa; fasta scores: opt: 163 z-score: 216.2 E(): 0.00014; 38.1% identity in 97 aa overlap,2SC7G11.39, possible magnesium chelatase (fragment), len: >248 aa; similar to SW:BCHI_CHLVI (EMBL:Z83933) Chlorobium vibrioforme magnesium-chelatase 38 kDa subunit (MG-protoporphyrin IX chelatase) BchI, 346 aa; fasta scores: opt: 441 z-score: 520.9 E(): 1.5e-21; 38.5% identity in 283 aa overlap. Contains Pfam match to entry PF01078 Mg_chelatase, Magnesium chelatase, subunit ChlI 3 3 10 21 [Reporter]SCO7020 (2C10)_PCR/5258 [Gene]SCO7020/4794 NC_003888 SC1H10.09, amlB, secreted alpha-amylase (EC 3.2.1.1), len: 506 aa. Highly similar to Streptomyces lividans TR:P97179(EMBL:Z85949) alpha-amylase, AmlB (573 aa), fasta scores opt: 3422 z-score: 3742.9 E(): 0 98.6% identity in 506 aa overlap and Streptomyces violaceus SW:AMY_STRVL(EMBL:U22894) alpha-amylase precursor (EC 3.2.1.1), (569 aa), fasta scores opt: 2682 z-score: 2933.9 E(): 0 77.1% identity in 502 aa overlap. Contains a Pfam match to entry PF00128 alpha-amylase, Alpha amylase and a possible N-terminal signal sequence. 3 3 10 20 [Reporter]SCO4716 (3O6)_PCR/5257 [Gene]SCO4716/4793 NC_003888 SCD31.41, rpsH, 30S ribosomal protein S8, len: 147 aa; identical to C-terminal part of SW:RS8_STRCO (EMBL:X83011) Streptomyces coelicolor 30S ribosomal protein S8 RpsH (fragment), 121 aa and highly similar to SW:RS8_BACHD (EMBL:AB017508) Bacillus halodurans 30S ribosomal protein S8 RpsH, 131 aa; fasta scores: opt: 526 z-score: 661.3 E(): 2.1e-29; 61.8% identity in 131 aa overlap. Contains Pfam match to entry PF00410 Ribosomal_S8, Ribosomal protein S8 3 3 10 19 [Reporter]SCO6624 (4K6)_PCR/5256 [Gene]SCO6624/4792 NC_003888 SC1F2.21c, putative membrane protein, len: 42 aa; hydrophobic in C-terminus. Contains possible hydrophobic membrane spanning regions 3 3 10 18 [Reporter]SCO5726 (5G6)_PCR/5255 [Gene]SCO5726/4791 NC_003888 SC3C3.12, unknown, len: 159 aa 3 3 10 17 [Reporter]SCO2042 (6C6)_PCR/5254 [Gene]SCO2042/4790 NC_003888 SC4G6.11c, possible membrane protein, len: 211aa; similar to TR:O06128 (EMBL:Z95554) hypothetical protein from Mycobacterium tuberculosis (235 aa) fasta scores; opt: 155, z-score: 174.0, E(): 0.023, (29.9% identity in 224 aa overlap). Contains possible membrane spanning hydrophobic regions. 2 1 22 13 [Reporter]SCO2439 (10B16)_PCR/978 [Gene]SCO2439/899 NC_003888 SCC24.10c, possible calcium-binding protein, len: 286 aa; similar to TR:BAA90694 (EMBL:AB037936) Xenopus laevis regucalcin, 299 aa; fasta scores: opt: 449 z-score: 514.7 E(): 3.1e-21; 33.8% identity in 293 aa overlap 2 1 22 12 [Reporter]SCO4559 (11N12)_PCR/977 [Gene]SCO4559/898 NC_003888 SCD16A.24c, possible electron transfer oxidoreductase, len: 430a a; similar to many e.g. TR:O06427 (EMBL:Z95558) hypothetical protein from Mycobacterium tuberculosis (408 aa) fasta scores; opt: 449, z-score: 501.6, E(): 1.3e-20, (34.6% identity in 416 aa overlap) and SW:FIXC_ECOLI probable electron transfer oxidoreductase FixC from Escherichia coli (428 aa) fasta scores; opt: 277, z-score: 311.0, E(): 5.4e-10, (27.0% identity in 367 aa overlap). 2 1 22 11 [Reporter]SCO4265 (12J12)_PCR/976 [Gene]SCO4265/897 NC_003888 SCD86A.02c, probable transport integral membrane protein, len: 503 aa; similar to SW:PUR8_STRLP (EMBL:X76855) Streptomyces lipmanii puromycin resistance protein Pur8, 503 aa; fasta scores: opt: 1772 z-score: 1875.8 E(): 0; 56.4% identity in 491 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and match to Prosite entry PS00216 Sugar transport proteins signature 1. Also contains possible hydrophobic membrane spanning regions 2 1 22 10 [Reporter]SCO3015 (13F12)_PCR/975 [Gene]SCO3015/896 NC_003888 SCE33.17, possible integral membrane protein, len: 462 aa; similar to TR:O69657 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical 46.4 kD protein MTV025.037, 451 aa; fasta scores: opt: 730 z-score: 506.4 E(): 9.5e-21; 38.1% identity in 412 aa overlap. Contains possible hydrophobic membrane spanning regions and high content in proline and glycine amino acid residues 2 1 22 9 [Reporter]SCO1183 (14B12)_PCR/974 [Gene]SCO1183/895 NC_003888 SCG11A.14, hypothetical protein, len:389 aa: similar to various hypothetical proteins, e.g. SW:YEHL_ECOLI (EMBL:U00007) Escherichia coli hypothetical 42.4 KD protein in MolR-BglX intergenic region, 384 aa; fasta scores: opt: 873 z-score: 979.4 E(): 0; 45.1% identity in 381 aa overlap 2 1 22 8 [Reporter]SCO0411 (15N8)_PCR/973 [Gene]SCO0411/894 NC_003888 SCF51.10, unknown, len: 74 aa. Contains a prosite match to entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 22 7 [Reporter]SCO4808 (16J8)_PCR/972 [Gene]SCO4808/893 NC_003888 SCD63A.19, sucC, succinyl-CoA synthetase beta chain, len: 394 aa; similar to SW:SUCC_ECOLI (EMBL:J01619) Escherichia coli succinyl-CoA synthetase beta chain (EC 6.2.1.5) SucC, 388 aa; fasta scores: opt: 873 z-score: 928.1 E(): 0; 40.6% identity in 394 aa overlap and to TR:O87840 (EMBL:AL031013) Streptomyces coelicolor succinyl-coa synthetase beta chain SucC1 SC8A6.06, 383 aa; fasta scores: opt: 983 z-score: 992.7 E(): 0; 60.3% identity in 393 aa overlap. Contains Pfam match to entry PF00549 ligase-CoA, CoA-ligases and match to Prosite entry PS01217 ATP-citrate lyase / succinyl-CoA ligases family signature 3 2 1 22 6 [Reporter]SCO3571 (17F8)_PCR/971 [Gene]SCO3571/892 NC_003888 SCH17.05, probable transcriptional regulator, len: 224 aa; similar to many, especially cAMP-binding receptors, e.g. TR:O34015 (EMBL:U87623), Crp, Vibrio cholerae cyclic AMP receptor protein (210 aa), fasta scores; opt: 314 z-score: 381.1 E(): 6.7e-14, 30.6% identity in 180 aa overlap. Highly similar to TR:O69644 (EMBL:AL022121) Mycobacterium tuberculosis putative transcriptional regulator (224 aa) (54.5% identity in 224 aa overlap). Contains probable helix-turn-helix motif at aa 176-197 (Score 1923, +5.74 SD). Contains Pfam matches to entry PF00027 cNMP_binding, Cyclic nucleotide-binding domain and to entry PF00325 crp, Bacterial regulatory proteins, crp family 2 1 22 4 [Reporter]SCO5341 (19N4)_PCR/969 [Gene]SCO5341/891 NC_003888 SCBAC5H2.10, spdA2 protein, len: 222 aa; highly similar to plasmid borne TR:Q07193 (EMBL:Z19593) Streptomyces ambofaciens probably involved in pSAM2 spreading (intramycelial transfer) SpdA protein, 224 aa; fasta scores: opt: 1086 z-score: 974.5 E(): 0; 74.9% identity in 223 aa overlap and to TR:Q9X8C9 (EMBL:AL049573) Streptomyces coelicolor SpdA protein, 217 aa; fasta scores: opt: 1079 z-score: 968.6 E(): 0; 75.5% identity in 220 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 22 3 [Reporter]SCO1292 (20J4)_PCR/968 [Gene]SCO1292/890 NC_003888 SCBAC36F5.03, putative secreted protein, len: 907 aa. Contains possible N-terminal region signal peptide sequence 4 4 5 7 [Reporter]SCO1839 (15H13)_PCR/7653 [Gene]SCO1839/6969 NC_003888 SCI8.24c, possible transcriptional regulator, len: 73 aa. Contains a possible helix-turn-helix motif between residues 32..53 (+3.35 SD). 4 4 5 6 [Reporter]SCO0746 (16D13)_PCR/7652 [Gene]SCO0746/6968 NC_003888 SCF81.05, possible serine/threonine protein kinase (putative secreted protein), len: 462 aa; low similarity to C-terminus SW:PKWA_THECU (EMBL:U23820) Thermomonospora curvata putative serine/threonine-protein kinase PkwA (EC 2.7.1.-), 742 aa; fasta scores: opt: 157 z-score: 167.3 E(): 0.06; 23.6% identity in 309 aa overlap. Contains possible N-terminal signal peptide sequence 4 4 5 5 [Reporter]SCO1693 (17P9)_PCR/7651 [Gene]SCO1693/6967 NC_003888 SCI30A.14c, unknown, len: 425aa; 4 4 5 4 [Reporter]SCO0903 (18L9)_PCR/7650 [Gene]SCO0903/6966 NC_003888 SCM1.36, unknown, len: 86 aa. 4 4 5 3 [Reporter]SCO4994 (19H9)_PCR/7649 [Gene]SCO4994/6965 NC_003888 2SCK36.17, unknown, len: 317 aa 4 4 4 22 [Reporter]SCO4539 (1P1)_PCR/7646 [Gene]SCO4539/6964 NC_003888 2SCD4.10c, hypothetical protein, len: 201 aa; highly similar to TR:CAB82060 (EMBL:AL161803) Streptomyces coelicolor hypothetical 23.3 kDa protein SCD31.17, 215 aa; fasta scores: opt: 776 z-score: 926.5 E(): 0; 59.0% identity in 200 aa overlap 4 4 4 21 [Reporter]SCO5373 (2L1)_PCR/7645 [Gene]SCO5373/6963 NC_003888 2SC6G5.17, atpD, ATP synthase beta chain (EC 3.6.1.34), len: 478aa; strongly similar to many eg. SW:P37809 (ATPB_BACSU) ATP synthase beta chain from Bacillus subtilis (473 aa) fasta scores; opt: 2051, z-score: 2236.9, E(): 0, 67.7% identity in 470 aa overlap. Identical to SW:P50004 (ATPB_STRLI) ATP synthase beta chain from Streptomyces lividans. Contains Pfam match to entry PF00006 ATP-synt_ab, ATP synthase alpha/beta family, Pfam match to entry PF00306 ATP-synt_ab_C, ATP synthase ab C terminal, Prosite match to PS00152 ATP synthase alpha and beta subunits signature and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 4 4 20 [Reporter]SCO4575 (3H1)_PCR/7644 [Gene]SCO4575/6962 NC_003888 SCD16A.08c, nuoN, NADH dehydrogenase subunit, len: 552 aa; similar to many e.g. TR:O53308 (EMBL:AL021646) NuoN, NADH dehydrogenase subunit from Mycobacterium tuberculosis (531 aa) fasta scores; opt: 1493, z-score: 1618.7, E(): 0, (56.7% identity in 543 aa overlap) and SW:NUON_ECOLI, NADH dehydrogenase subunit NuoN from Escherichia coli (425 aa) fasta scores; opt: 685, z-score: 746.4, E(): 0, (39.8% identity in 389 aa overlap). Contains Pfam match to entry PF00361 oxidored_q1, NADH-Ubiquinone/plastoquinone (complex I), various chains. Contains possible membrane spanning hydrophobic regions. 3 3 10 16 [Reporter]SCO3608 (7O2)_PCR/5253 [Gene]SCO3608/4789 NC_003888 SC66T3.19c, hypothetical protein, len: 169 aa; unknown function, weakly similar to TR:O32077 (EMBL:Z99119), YuaF, Bacillus subtilis hypothetical protein (174 aa), fasta scores; opt: 142 z-score: 164.3 E(): 0.08, 28.0% identity in 161 aa overlap. Contains 5x (V/L)(F/L)(D/E)G repeats near the N-terminus 4 4 4 19 [Reporter]SCO6157 (4D1)_PCR/7643 [Gene]SCO6157/6961 NC_003888 SC1A9.21c, possible transmembrane protein, len: 354 aa; contains possible membrane spanning hydrophobic domains in the N-terminal region and possible coiled-coil from 221 to 250 (30 residues) Max score: 1.449 (probability 0.87) 3 3 10 15 [Reporter]SCO5568 (8K2)_PCR/5252 [Gene]SCO5568/4788 NC_003888 SC7A1.12, coaD, phosphopantetheine adenylyltransferase, len: 159 aa; strong similarity to many including SW:COAD_ECOLI phosphopantetheine adenylyltransferase (EC 2.7.7.3) CoaD or KdtB or B3634 from Escherichia coli (159 aa) fasta scores: opt: 485 Z-score: 587.5 E(): 4.5e-25; 45.513% identity (45.806% ungapped) in 156 aa overlap 4 4 4 18 [Reporter]SCO6231 (4P21)_PCR/7642 [Gene]SCO6231/6960 NC_003888 SC2H4.13c, probable sugar transport system sugar-binding lipoprotein, len: 440 aa; some similarity to several, e.g. TR:Q46443 (EMBL:M57692) Thermoanaerobacterium thermosulfurigenes maltose-binding protein precursor (446 aa), fasta scores; opt: 239 z-score: 192.4 E(): 0.0018, 23.5% identity in 366 aa overlap. Contains N-terminal signal sequence with appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site sugar transport system sugar-binding lipoprotein 3 3 10 14 [Reporter]SCO5675 (9G2)_PCR/5251 [Gene]SCO5675/4787 NC_003888 SC8B7.03c, unknown, len: 256 aa 3 3 10 13 [Reporter]SCO6484 (10C2)_PCR/5250 [Gene]SCO6484/4786 NC_003888 SC9C7.20, conserved hypothetical protein, len: 860aa; similar to many hypothetical proteins egs. TR:O86875 (EMBL:U22894) from Streptomyces lividans (547 aa) fasta scores; opt: 1000, z-score: 738.2, E(): 0, (40.0% identity in 497 aa overlap) and TR:O86807 (EMBL:AL031031) from Streptomyces coelicolor (916 aa) fasta scores; opt: 358, z-score: 316.9, E(): 2.3e-10, (27.2% identity in 779 aa overlap). Also contains a short region of similarity to TR:Q53897 (EMBL:X60316) AbaA regulatory locus from Streptomyces coelicolor (192 aa) fasta scores; opt: 108, z-score: 223.2, E(): 3.8e-05, (38.5% identity in 135 aa overlap). 3 3 10 12 [Reporter]SCO2766 (10O22)_PCR/5249 [Gene]SCO2766/4785 NC_003888 SCC57A.37, possible secreted ribonuclease, len: 286 aa. Highly similar to Bacillus subtilis SW:RN_BACSU (EMBL:D01097) ribonuclease precursor (EC 3.1.-.-) (289 aa), fasta scores opt: 1165 z-score: 1237.7 E():0 61.5% identity in 273 aa overlap. Contains a possible N-terminal signal sequence rich in the amino acid Ala. 3 3 10 11 [Reporter]SCO4261 (11K22)_PCR/5248 [Gene]SCO4261/4784 NC_003888 SCD49.02c, possible response regulator, len: 219 aa; similar to TR:O53646 (EMBL:AL021928) Mycobacterium tuberculosis putative response regulator MTV0033.03, 211 aa; fasta scores: opt: 383 z-score: 449.9 E(): 1.4e-17; 35.6% identity in 208 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family 3 3 10 10 [Reporter]SCO3388 (12G22)_PCR/5247 [Gene]SCO3388/4783 NC_003888 SCE126.06c, TmrB-like protein, len: 213aa; similar to SW:TMRB_BACSU tunicamycin resistance protein (binds tunicamycin) from Bacillus subtilis (197 aa) fasta scores; opt: 335, z-score: 416.0, E(): 7.3e-16, (38.9% identity in 203 aa overlap). 3 3 10 9 [Reporter]SCO1786 (13C22)_PCR/5246 [Gene]SCO1786/4782 NC_003888 SCI51.26c, probable iron-siderophore uptake system transmembrane component, len: 375 aa; similar to many transmembrane components from binding-protein-dependent iron uptake systems using a range of siderophores e.g. SW:FEPG_ECOLI (EMBL:X57471), fepG, Escherichia coli ferric enterobactin transport protein (330 aa), fasta scores; opt: 946 z-score: 1034.3 E(): 0, 50.0% identity in 310 aa overlap. Similar to neighbouring SCI51.27c, probable iron-siderophore uptake system transmembrane component (348 aa) (37.9% identity in 348 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF01032 FecCD_family, FecCD transport family 3 3 10 8 [Reporter]SCO3307 (14O18)_PCR/5245 [Gene]SCO3307/4781 NC_003888 SCE68.05c, conserved hypothetical protein, len: 300 aa; unknown function, similar to others from S.coelicolor e.g. TR:O86541 (EMBL:AL031350), SC1F3.04, hypothetical protein (261 aa), fasta scores; opt: 791 z-score: 916.0 E(): 0, 48.9% identity in 262 aa overlap 3 3 10 7 [Reporter]SCO3411 (15K18)_PCR/5244 [Gene]SCO3411/4780 NC_003888 SCE9.18c, possible membrane protein, len: 303 aa; similar to many hypothetical proteins e.g. SW:DEDA_ECOLI (EMBL:M68934), dedA, Escherichia coli hypothetical protein in hisT-purF region (219 aa), fasta scores; opt: 588 z-score: 585.6 E(): 2.7e-25, 49.7% identity in 187 aa overlap. Similar to others from S.coelicolor e.g. TR:O86627 (EMBL:AL031155) DedA family hypothetical protein (228 aa) (41.4% identity in 203 aa overlap). Central region contains hydrophobic, possible membrane spans and C-terminal region contains glutamine-rich repeats. Contains Pfam match to entry PF00597 DedA, DedA family, score 104.00, E-value 2.9e-27 2 1 21 22 [Reporter]SCO1097 (1B24)_PCR/965 [Gene]SCO1097/889 NC_003888 2SCG4.13, probable hydrolase, len: 292 aa; similar to TR:O52866 (EMBL:AJ224332) Corynebacterium sp. soluble epoxide hydrolase (EC 3.3.2.3) Ceh, 285 aa; fasta scores: opt: 601 z-score: 728.8 E(): 0; 35.8% identity in 288 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 2 1 21 21 [Reporter]SCO2087 (2N20)_PCR/964 [Gene]SCO2087/888 NC_003888 SC4A10.20c, murX, probable phospho-N-acetylmuramoyl-pentapeptide-transferase, len: 363 aa; similar to many e.g. SW:MRAY_ECOLI (EMBL:X51584), MraY, MurX, Escherichia coli phospho-N-acetylmuramoyl-pentapeptide-transferase involved in peptidoglycan biosynthesis (360 aa), fasta scores; opt: 615 z-score: 714.6 E(): 1.8e-32, 43.3% identity in 344 aa overlap. Also similar to TR:O69555 (EMBL:AL022602), MurX, Mycobacterium leprae probable phospho-N-acetylmuramoyl-pentapeptide-transferase (359 aa) (51.8% identity in 361 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF00953 Glycos_transf_4, Glycosyl transferase 2 1 21 20 [Reporter]SCO1383 (3J20)_PCR/963 [Gene]SCO1383/887 NC_003888 SC1A8A.03c, hypothetical protein, len: 246aa; similar to SW:Q50605 (Y0DF_MYCTU) hypothetical protein from Mycobacterium tuberculosis (247 aa) fasta scores; opt: 359, z-score: 418.8, E(): 6.8e-16, 42.2% identity in 230 aa overlap. 2 1 21 19 [Reporter]SCO5864 (4F20)_PCR/962 [Gene]SCO5864/886 NC_003888 SC2E9.05, unknown, len: 98 aa; highly similar to hypothetical proteins from Mycobacterium tuberculosis and M. leprae eg. TR:O07201 (EMBL:Z96072) MTCY05A6.20C (100 aa), fasta scores; opt: 465 z-score: 814.5 E(): 0, 71.4% identity in 98 aa overlap 2 1 21 18 [Reporter]SCO7107 (5B20)_PCR/961 [Gene]SCO7107/885 NC_003888 SC4B10.08c, conserved hypothetical protein, len: 198 aa; similar to TR:O86515 (EMBL:AL031124) Streptomyces coelicolor hypothetical 20.0 kDa protein SC1C2.14c, 185 aa; fasta scores: opt: 698 z-score: 894.2 E(): 0; 56.9% identity in 174 aa overlap 2 1 21 17 [Reporter]SCO2178 (6N16)_PCR/960 [Gene]SCO2178/884 NC_003888 SC5F7.23c, possible endo alpha-1,4 polygalactosaminidase, possibly secreted, len: 282 aa; similar to TR:Q52423 (EMBL:D14846) endo alpha-1,4 polygalactosaminidase from Pseudomonas sp.881 (294 aa) fasta scores; opt: 759, z-score: 748.2, E(): 0, (44.2% identity in 278 aa overlap). Contains possible N-terminal signal sequence 2 1 21 16 [Reporter]SCO2111 (7J16)_PCR/959 [Gene]SCO2111/883 NC_003888 SC6E10.05, probable endonuclease, len: 294 aa; similar to many DNA-repair endonucleases e.g. SW:END4_ECOLI (EMBL:M22591), nfo, Escherichia coli endonuclease IV (285 aa), fasta scores; opt: 482 z-score: 516.5 E(): 1.9e-21, 33.3% identity in 279 aa overlap and SW:APN1_YEAST (EMBL:M33667), apn1, Saccharomyces cerevisiae DNA-(apurinic or apyrimidinic site) lyase (366 aa) (28.4% identity in 289 aa overlap). Contains Pfam match to entry PF01261 AP_endonuclease2, AP endonuclease family 2, PS00730 AP endonucleases family 2 signature 2 and PS00731 AP endonucleases family 2 signature 3 2 1 21 15 [Reporter]SCO6849 (8F16)_PCR/958 [Gene]SCO6849/882 NC_003888 SC7F9.01c, possible membrane protein, partial CDS, len: > 373 aa. Highly similar to Streptomyces coelicolor TR:Q9X8A2(EMBL:AL049645) hypothetical 45.0 KD protein, SCE2.07 (427 aa), fasta scores opt: 1024 z-score: 1198.6 E():0 68.8% identity in 224 aa overlap. Contains a possible membrane spanning hydrophobic domain.,SC3D9.17c, unknown, partial CDS, len:> 235 aa. Highly similar to Streptomyces coelicolor TR:Q9X8A2(EMBL:AL049645) hypothetical protein, SCE2.07 (427 aa), fasta scores opt: 1120 z-score: 1326.9 E():0 71.8% identity in 234 aa overlap. 2 1 21 14 [Reporter]SCO7540 (9B16)_PCR/957 [Gene]SCO7540/881 NC_003888 SC8G12.16c, hypothetical protein, len: 223 aa. Similar to many proteins of unknown function e.g. Streptomyces coelicolor TR:CAB53137(EMBL:AL109962) conserved hypothetical protein, SCJ1.19C (223 aa), fasta scores: opt: 425 z-score: 510.8 E(): 5.4e-21 40.5% identity in 220 aa overlap. Contains 2x Pfam matches to entry PF00571 CBS, CBS domain 2 1 21 13 [Reporter]SCO1919 (10N12)_PCR/956 [Gene]SCO1919/880 NC_003888 SCC22.01c, partial CDS, conserved hypothetical protein, len: >75aa; similar to many other hypothetical proteins eg. TR:O53157 (EMBL:AL021184) hypothetical protein from Mycobacterium tuberculosis (115 aa) fasta scores; opt: 284, z-score: 392.0, E(): 1.7e-14, (61.3% identity in 80 aa overlap).,SCI7.37c, partial CDS, unknown, len: >74aa; similar to TR:O53157 (EMBL:AL021184) hypothetical protein from Mycobacterium tuberculosis (115 aa) fasta scores; opt: 375, z-score: 536.1, E(): 1.6e-22, (70.1% identity in 77 aa overlap). 4 4 4 17 [Reporter]SCO7051 (5L21)_PCR/7641 [Gene]SCO7051/6959 NC_003888 SC4G1.17c, probable transcription terminator factor, len: 415 aa; similar to C-terminal part of SW:RHO_STRLI (EMBL:X95444) Streptomyces lividans transcription termination factor Rho, 707 aa; fasta scores: opt: 1185 z-score: 1296.6 E(): 0; 54.8% identity in 383 aa overlap and to TR:CAB94528 (EMBL:AL359152) Streptomyces coelicolor rho, transcription termination factor, partial CDS 2St6G5.01, 383 aa; fasta scores: opt: 1187 z-score: 1150.1 E(): 0; 56.5% identity in 368 aa overlap. Contains Pfam match to entry PF00006 ATP-synt_ab, ATP synthase alpha/beta family and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 4 4 15 [Reporter]SCO7420 (7D21)_PCR/7639 [Gene]SCO7420/6958 NC_003888 SC6D11.16c, cvnC10, unknown, len: 115 aa. Highly similar to many proteins of undefined function from Streptomyces coelicolor including: TR:CAB61280 (EMBL:AL132991) SCF55.10c. (113 aa), fasta scores opt: 407 z-score: 530.3 E(): 4.1e-22 63.1% identity in 111 aa overlap and TR:Q9X834 (EMBL:AL049727) SC9B1.14C (119 aa), fasta scores opt: 249 z-score: 330.7 E(): 5.3e-11 46.2% identity in 117 aa overlap. 4 4 4 13 [Reporter]SCO6132 (9L17)_PCR/7637 [Gene]SCO6132/6957 NC_003888 SC9B2.19c, possible integral membrane protein, len: 198 aa; contains several possible membrane spanning regions. 4 4 4 12 [Reporter]SCO2453 (10H17)_PCR/7636 [Gene]SCO2453/6956 NC_003888 SCC24.24, unknown, len: 72 aa. High content in glycine and proline amino acid residues 4 4 4 11 [Reporter]SCO4357 (11D17)_PCR/7635 [Gene]SCO4357/6955 NC_003888 SCD19.12, possible integral membrane protein, len: 348 aa. Contains possible hydrophobic membrane spanning regions 4 4 4 10 [Reporter]SCO3519 (12P13)_PCR/7634 [Gene]SCO3519/6954 NC_003888 SCE134.20, unknown, len: 203aa; 4 4 4 9 [Reporter]SCO0040 (13L13)_PCR/7633 [Gene]SCO0040/6953 NC_003888 SCJ4.21, possible glycosyl transferase, len: 407 aa; similar to many e.g. TR:O86304 (EMBL:AJ223970) macrolide glycosyl transferase from Streptomyces ambofaciens (417 aa) fasta scores; opt: 615, z-score: 696.4, E(): 2e-31, (34.3% identity in 405 aa overlap). Contains Pfam match to entry PF00201 UDPGT, UDP-glucoronosyl and UDP-glucosyl transferases. 4 4 4 8 [Reporter]SCO4945 (14H13)_PCR/7632 [Gene]SCO4945/6952 NC_003888 2SCK31.05, probable dehydrogenase, len: 346 aa; highly similar to SW:ADH_MYCTU (EMBL:AL021287) Mycobacterium bovis NADP-dependent alcohol dehydrogenase AdhC, 346 aa; fasta scores: opt: 1508 z-score: 1698.9 E(): 0; 64.5% identity in 346 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and matches to Prosite entries PS00059 Zinc-containing alcohol dehydrogenases signature and PS00017 ATP/GTP-binding site motif A (P-loop) 3 3 10 6 [Reporter]SCO3788 (16G18)_PCR/5243 [Gene]SCO3788/4779 NC_003888 SCH63.35, unknown, len: 136 aa; similar to TR:Q98LX4 (EMBL:AP002995) hypothetical protein from Rhizobium loti (Mesorhizobium loti) (71 aa) fasta scores; 78.689% identity (78.689% ungapped) in 61 aa overlap. 4 4 4 7 [Reporter]SCO0832 (15D13)_PCR/7631 [Gene]SCO0832/6951 NC_003888 SCF43A.22c, hypothetical protein, len: 321 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Similar to the adjacent protein SCF43A.21c, hypothetical protein (306 aa), fasta scores; opt: 674 z-score: 666.6 E(): 5.5e-32, 41.3% identity in 300 aa overlap 3 3 10 5 [Reporter]SCO1717 (17C18)_PCR/5242 [Gene]SCO1717/4778 NC_003888 SCI11.06, hypothetical protein, len: 391 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 4 4 4 6 [Reporter]SCO0682 (16P9)_PCR/7630 [Gene]SCO0682/6950 NC_003888 SCF15.03c, unknown, len: 124 aa. Contains a probable coiled-coil between residues 96..124 (29 residues). 3 3 10 4 [Reporter]SCP1.26c (18O14)_PCR/5241 [Gene]SCP1.26c/4777 NC_003888 SCP1.26c, unknown, len: 280aa; 3 3 10 3 [Reporter]SCO1297 (19K14)_PCR/5240 [Gene]SCO1297/4776 NC_003888 SCBAC36F5.08c, unknown, len: 173 aa 3 3 10 2 [Reporter]SCO5103 (20C14)_PCR/5239 [Gene]SCO5103/4775 NC_003888 SCBAC28G1.29, possible regulatory protein, len: 155 aa; similar to TR:Q53897 (EMBL:X60316) Streptomyces coelicolor AbaA gene (putative regulator) or SCF42.12c, 192 aa; fasta scores: opt: 207 Z-score: 233.7 bits: 49.7 E(): 2.1e-05; 41.270% identity in 126 aa overlap. Contains Pfam match to entry PF02518 HATPase_c, Histidine kinase-, DNA gyrase B-, phytochrome-like ATPase 3 3 9 22 [Reporter]SCO5273 (1C10)_PCR/5237 [Gene]SCO5273/4774 NC_003888 2SC7G11.35, hypothetical protein, len: 620 aa; N-terminal region similar to SW:ASLB_ECOLI (EMBL:M87049) Escherichia coli putative arylsulfatase regulatory protein AslB, 411 aa; blastp (consecutive) scores: Score= 166 (58.4 bits), Expec = 2.6e-19, Sum P(2)= 2.6e-19, Identities= 57/184 (30%), Positives= 82/184 (44%) and Score= 141 (49.6 bits), Expect= 2.6e-19, Sum P(2 = 2.6e-19, Identities= 47/165 (28%), Positives= 73/165 (44%). Contains Pfam match to entry PF01444 MoaA_NifB_PqqE, moaA / nifB / pqqE family 3 3 9 21 [Reporter]SCO1847 (2O6)_PCR/5236 [Gene]SCO1847/4773 NC_003888 SCI8.32, cobD, cobalamin biosynthesis protein, len: 327 aa. Highly similar to many CobD family proteins involved in cobalamin (vitamin B12) biosynthesis e.g. Salmonella typhimurium SW:CBIB_SALTY (EMBL: L12006) CbiB protein (319 aa), fasta scores opt: 527 z-score: 566.6 E(): 3.5e-24 36.1% identity in 305 aa overlap and Mycobacterium tuberculosis SW:COBD_MYCTU (EMBL: Z70692) cobalamin biosynthesis protein (313 aa), fasta scores opt: 991 z-score: 1057.1 E():0 54.2% identity in 308 aa overlap. Contains a possible membrane spanning hydrophobic domain. 3 3 9 20 [Reporter]SCO4655 (3K6)_PCR/5235 [Gene]SCO4655/4772 NC_003888 SCD82.27, rpoC, DNA-directed RNA polymerase beta' chain (fragment), len: >1058 aa; highly similar to SW:RPOC_MYCTU (EMBL:L27989) Mycobacterium tuberculosis DNA-directed RNA polymerase beta' chain (EC 2.7.7.6) RpoC, 1316aa; fasta scores: opt: 5139 z-score: 5536.5 E(): 0; 72.9% identity in 1067 aa overlap. Contains Pfam match to entry PF00623 RNA_pol_A, RNA polymerase alpha subunit. Contains also possible coiled-coil region aprox. at residues 168..220,SCD40A.01, rpoC, DNA-directed RNA polymerase beta' chain (fragment), len: >279 aa; similar to C-terminal region of SW:RPOC_BACSU (EMBL:L43593) Bacillus subtilis DNA-directed RNA polymerase beta' chain (EC 2.7.7.6) RpoC, 1199 aa; fasta scores: opt:801 z-score: 911.4 E(): 0; 53.0% identity in 247 aa overlap 2 2 16 5 [Reporter]SCO0463 (17J23)_PCR/2854 [Gene]SCO0463/2599 NC_003888 SCF76.03, unknown, len: 204 aa; similar to TR:Q9L1U0 (EMBL:AL138851) SCE59.14C hypothetical protein from Streptomyces coelicolor (196 aa) fasta scores; opt: 357, Z-score: 418.9, E(): 1.1e-15, 37.433% identity (40.230% ungapped) in 187 aa overlap. 3 3 9 19 [Reporter]SCO6045 (4G6)_PCR/5234 [Gene]SCO6045/4771 NC_003888 SC1B5.05c, unknown, len: 438 aa; slight similarity to hypothetical protein from Haemophilus influenzae YG00_HAEIN P44268 (317 aa), fasta scores; opt: 252 z-score: 283.4 E(): 1.5e-08, 26.8% identity in 298 aa overlap 2 2 16 4 [Reporter]SCO0871 (18F23)_PCR/2853 [Gene]SCO0871/2598 NC_003888 SCM1.04c, possible two-component sensor protein. Len: 545 aa. Similar, in parts, to many others including: Methanobacterium thermoautotrophicum TR:O26544(EMBL:AE000828) signal-transducing histidine kinase (373 aa), fasta scores opt: 509 z-score: 548.0 E(): 3.5e-23 34.2% identity in 313 aa overlap and Streptomyces coelicolor TR:O88056(EMBL:AL031541) putative two-component sensor (379 aa), fasta scores opt:350 z-score: 378.5 E(): 9.7e-14 35.6% identity in 233 aa overlap. Contains a Pfam matches to entries PF00512 signal, Histidine kinase and Pfam match to entry PF00672 DUF5, Domain found in bacterial signal proteins. Also contains possible membrane spanning hydrophobic regions 3 3 9 18 [Reporter]SCO5721 (5C6)_PCR/5233 [Gene]SCO5721/4770 NC_003888 SC3C3.07, unknown, len: 508 aa; similar to three hypothetical proteins from M. tuberculosis e.g. TR:O06317 (EMBL:Z95390) MTCY13E12.03C (470 aa), fasta scores; opt: 213 z-score: 330.7 E(): 3.7e-11, 28.0% identity in 508 aa overlap. Contains possible transmembrane domain at N-terminus 2 1 21 12 [Reporter]SCO4577 (11J12)_PCR/955 [Gene]SCO4577/879 NC_003888 SCD16A.06c, probable helicase, len: 676aa; similar to many (both prokaryote and eukaryote) egs. TR:AAD05424 (EMBL:AF047374) RecQ helicase from Neisseria gonorrhoeae (767 aa) fasta scores; opt: 1809, z-score: 1769.1, E(): 0, (50.4% identity in 601 aa overlap) and SW:HUS2_SCHPO ATP-dependent helicase from Schizosaccharomyces pombe (1328 aa) fasta scores; opt: 1013, z-score: 988.7, E(): 0, (40.2% identity in 493 aa overlap). Contains Pfam match to entry PF00271 helicase_C, Helicases conserved C-terminal domain, score 78.40, E-value 1.4e-19. 2 2 16 3 [Reporter]SCO6107 (19B23)_PCR/2852 [Gene]SCO6107/2597 NC_003888 SCBAC1A6.31, possible integral membrane protein, len: 344 aa; similar to TR:O07208 (EMBL:Z96072) Mycobacterium tuberculosis hypothetical 35.0 kDa protein MTCY05A6.28, 324 aa; fasta scores: opt: 593 z-score: 664.2 E(): 2e-29; 36.2% identity in 268 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 21 11 [Reporter]SCO4483 (12F12)_PCR/954 [Gene]SCO4483/878 NC_003888 SCD69.03, hypothetical protein, len: 200 aa; similar to TR:Q9Z582 (EMBL:AL035569) Streptomyces coelicolor SC8D9.09 protein, 164 aa; fasta scores: opt: 246 z-score: 291.3 E(): 9.1e-09; 34.1% identity in 132 aa overlap 2 2 16 2 [Reporter]SCO7500 (20J19)_PCR/2851 [Gene]SCO7500/2596 NC_003888 SCBAC17A6.33c, possible allantoin permease, len: 495aa; similar to many eg. SW:P75712 (ALLP_ECOLI) putative allantoin permease from Escherichia coli (484 aa) fasta scores: opt: 807, Z-score: 872.9, 29.622% identity (30.454% ungapped) in 476 aa overlap. Contains Pfam match to entry PF02133 Transp_cyt_pur, Permease for cytosine/purines, uracil, thiamine, allantoin and multiple hydrophobic possible membrane spanning regions. 2 1 21 10 [Reporter]SCO3011 (13B12)_PCR/953 [Gene]SCO3011/877 NC_003888 SCE33.13c, possible lipoprotein, len: 615 aa; similar to TR:O05889 (EMBL:Z95121) Mycobacterium tuberculosis hypothetical 61.2 kD protein MTCY20B11.19c, 583 aa; fasta scores: opt: 228 z-score: 243.7 E(): 4.1e-06; 25.5% identity in 624 aa overlap. Contains properly located match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 2 2 15 22 [Reporter]SCO1217 (1J15)_PCR/2849 [Gene]SCO1217/2595 NC_003888 2SCG58.17c, possible sensor kinase, len: 405 aa; similar to TR:AAF31453 (EMBL:AF221126) Streptococcus pneumoniae putative histidine kinase ZmpS, 563 aa; fasta scores: opt: 375 z-score: 453.5 E(): 8.6e-18; 26.7% identity in 363 aa overlap. Contains Pfam match to entry PF00512 signal, Histidine kinase and possible N-terminal region signal peptide sequence (possibly cleavable) 2 1 21 9 [Reporter]SCO0131 (14N8)_PCR/952 [Gene]SCO0131/876 NC_003888 SCJ21.12c, probable secreted protein, len: 279 aa; similar to hypothetical proteins e.g. YBHP_ECOLI (253 aa), fasta scores; opt: 244 z-score: 290.0 E(): 8.4e-09, 29.5% identity in 251 aa overlap. Also similar to S. coelicolor TR:Q9ZBN3 (EMBL:AL034492) SC6C5.12C (276 aa), fasta scores; opt: 474 z-score: 555.0 E(): 1.5e-23, 41.0% identity in 278 aa overlap 2 2 15 21 [Reporter]SCO4683 (2F15)_PCR/2848 [Gene]SCO4683/2594 NC_003888 SCD31.08, gdhA, NADP-specific glutamate dehydrogenase, len: 461 aa; similar to TR:O87403 (EMBL:AF056335) Bacillus licheniformis NADP-specific glutamate dehydrogenase (EC 1.4.1.4) GdhA, 460 aa; fasta scores: opt: 1759 z-score: 1940.3 E(): 0; 59.1% identity in 435 aa overlap and to SW:DHE4_ECOLI (EMBL:J01615) Escherichia coli NADP-specific glutamate dehydrogenase (EC 1.4.1.4) GdhA, 447 aa; fasta scores: opt: 1683 z-score: 1856.8 E(): 0; 58.7% identity in 446 aa overlap. Contains Pfam match to entry PF00208 GLFV_dehydrog, Glutamate/Leucine/Phenylalanine/Valine dehydrogenase and match to Prosite entry PS00074 Glu / Leu / Phe / Val dehydrogenases active site 2 1 21 8 [Reporter]SCO0535 (15J8)_PCR/951 [Gene]SCO0535/875 NC_003888 SCF11.15, possible hydrolase (putative secreted protein), len: 473 aa; similar to TR:AAD30364 (EMBL:AF078737) Caldicellulosiruptor sp. Tok7B.1 xylanase/endoglucanase, CelB, 1426 aa; fasta scores: opt: 484 z-score: 506.2 E(): 7.9e-21; 33.0% identity in 327 aa overlap. Contains a Pfam match to entry PF00942 CBD_3, cellulose binding domain. Contains possible n-terminal region signal sequence peptide 2 2 15 20 [Reporter]SCO2226 (3B15)_PCR/2847 [Gene]SCO2226/2593 NC_003888 SC10B7.21c, probable bi-functional protein (secreted alpha-amylase/dextrinase), len: 1798 aa; N-terminal identical to SW:AMY_STRLI (EMBL:X70255) Streptomyces lividans alpha-amylase precursor (EC 3.2.1.1) Amy, 919 aa and C-terminal similar to TR:O48541 (EMBL:AF022725) Hordeum vulgare (Barley) limit dextrinase HVLD99, 904 aa; fasta scores: opt: 2114 z-score: 2193.6 E(): 0; 42.4% identity in 887 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase. Contains also possible N-terminal region signal peptide sequence 2 1 21 7 [Reporter]SCO3650 (16F8)_PCR/950 [Gene]SCO3650/874 NC_003888 SCH10.28c, pyrE, probable uridine 5'-monophosphate synthase, len: 182 aa; similar to many e.g. SW:PYRE_BACCL (EMBL:X73308), PyrE, Bacillus caldolyticus uridine 5'-monophosphate synthase (206 aa), fasta scores; opt: 242 z-score: 289.3 E(): 8.4e-09, 37.9% identity in 174 aa overlap. Contains Pfam match to entry PF00156 Pribosyltran, Purine/pyrimidine phosphoribosyl transferases, score -8.60, E-value 0.0025 2 2 15 18 [Reporter]SCO2099 (5J11)_PCR/2845 [Gene]SCO2099/2592 NC_003888 SC4A10.32, hypothetical protein, len: 168 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 2 1 21 6 [Reporter]SCO0476 (17B8)_PCR/949 [Gene]SCO0476/873 NC_003888 SCF76.16, ABC transport protein, ATP-binding subunit, len: 252 aa. Similar to many e.g. Haemophilus influenzae SW:FECE_HAEIN (EMBL; U32720) iron(III) dicitrate transport ATP-binding protein FecE homolog (306 aa), fasta scores opt: 375 z-score: 413.4 E(): 1.2e-15 37.3% identity in 185 aa overlap. Contains a Prosite hit to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 2 2 15 17 [Reporter]SCO5800 (6F11)_PCR/2844 [Gene]SCO5800/2591 NC_003888 SC4H2.21, unknown, len: 648 aa; similar to proteins implicated in siderophore biosynthesis eg. SW:RHSC_RHIME (EMBL:AF110737) rhizobactin siderophore biosynthesis protein RhsC from Rhizobium meliloti (585 aa) fasta scores; opt: 1215, Z-score: 1075.6, E(): 3.6e-53, 40.000% identity (41.912% ungapped) in 570 aa overlap. Very weak similarity to TR:Q47316 (EMBL:X76100) E. coli plasmid pColV-K30 iucA gene (575 aa), fasta scores; opt: 695 z-score: 458.0 E(): 2.6e-18, 30.1% identity in 555 aa overlap. Contains PS00136 Serine proteases, subtilase family, aspartic acid active site 2 1 21 5 [Reporter]SCO1830 (18N4)_PCR/948 [Gene]SCO1830/872 NC_003888 SCI8.15, unknown, len: 595 aa. Similar to several proteins of unknown function from Streptomyces coelicolor, including TR:CAB52841 (EMBL: AL109848) hypothetical 68.4 KD protein SCI51.17 (612 aa), fasta scores pt: 2473 z-score: 2932.7 E(): 0 60.7% identity in 596 aa overlap and TR:CAB42080 (EMBL: AL049763) conserved hypothetical protein SCE36.11C (612 aa) fasta scores opt: 1389 z-score: 1396.7 E():0 45.8% identity in 605 aa overlap. As well as Mycobacterium tuberculosis TR:P71741 (EMBL; Z81368) hypothetical 72.7 KD protein (642 aa), fasta scores opt: 1152 z-score: 1364.6 E(): 0 39.9% identity in 622 aa overlap. 2 2 15 16 [Reporter]SCO5693 (7B11)_PCR/2843 [Gene]SCO5693/2590 NC_003888 SC5H4.17, probable acyl CoA dehydrogenase, len: 658 aa; similar to SW:ACDV_BOVIN (EMBL:U30817) Bos taurus acyl-CoA dehydrogenase, very-long-chain specific precursor (EC 1.3.99.-) VlcaD, 655 aa; fasta scores: opt: 768 z-score: 864.8 E(): 0; 31.0% identity in 616 aa overlap . Contains 2x Pfam matches to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase 2 1 21 4 [Reporter]SCO7462 (19J4)_PCR/947 [Gene]SCO7462/871 NC_003888 SCBAC14E8.02c, possible integral membrane protein, len: 255 aa; similar to TR:Q49657 (EMBL:U00011) Mycobacterium leprae hypothetical 27.1 kDa protein U1177B precursor MLCL581.28c, 260 aa; fasta scores: opt: 670 Z-score: 780.5 bits: 152.0 E(): 6.8e-36; 47.059% identity in 255 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 21 3 [Reporter]SCO2239 (20F4)_PCR/946 [Gene]SCO2239/870 NC_003888 SC1G2.01c, possible secreted protein, partial CDS, len: >92 aa. Weakly similar to a protein of unknown function from Synechocystis sp. (strain PCC 6803) TR:Q55943(EMBL:D64005) (196 aa), fasta scores opt: 109 z-score: 144.6 E():1.1 28.9% identity in 83 aa overlap. Contains a a possible N-terminal signal sequence.,SCBAC17D6.06c, conserved hypothetical protein (fragment), len: >149 aa; similar to TR:Q9RVD0 (EMBL:AE001960) Deinococcus radiodurans conserved hypothetical protein DR1099, 197 aa; fasta scores:opt: 179 Z-score: 226.9 bits: 48.4 E(): 4.7e-05; 35.821% identity in 134 aa overlap 4 4 4 5 [Reporter]SCO0200 (17L9)_PCR/7629 [Gene]SCO0200/6949 NC_003888 SCJ12.12c, unknown, len: 301 aa. Similar to several hypothetical proteins including: Mycobacterium tuberculosis SW:YW23_MYCTU (EMBL; Z74025) hypothetical 33.9 KD protein MTCY39.23C (317 aa), fasta scores opt: 380 z-score: 447.3 E(): 1.5e-17 33.5% identity in 316 aa overlap and S. coelicolor (EMBL;AL109962) SCJ1.30c (328 aa)fasta scores opt: 1274 z-score: 1163.7 E():0 65.3% identity in 300 aa overlap. Contains 2x Pfam match to entry PF00582 Usp, Universal stress protein family. 4 4 4 4 [Reporter]SCO0151 (18H9)_PCR/7628 [Gene]SCO0151/6948 NC_003888 SCJ33.15, possible integral membrane protein, len: 112 aa. Contains Contains possible membrane spanning hydrophobic domains. 4 4 4 3 [Reporter]SCP1.302 (19D9)_PCR/7627 [Gene]SCP1.302/6947 NC_003888 SCP1.302, possible lysase, len: 287aa; similar to many eg. SW:P17725 (CILB_KLEPN) citrate lyase beta chain (EC 4.1.3.6) (289 aa) fasta scores; opt: 392, z-score: 446.6, E(): 2.1e-17, 31.5% identity in 286 aa overlap. 4 4 4 2 [Reporter]SCP1.123 (20L5)_PCR/7626 [Gene]SCP1.123/6946 NC_003888 SCP1.123, doubtful CDS, unknown, len: 41aa; 4 4 3 22 [Reporter]SCO4535 (1L1)_PCR/7624 [Gene]SCO4535/6945 NC_003888 2SCD4.06, possible integral membrane protein, len: 442 aa. Contains possible hydrophobic membrane spanning regions 4 4 3 21 [Reporter]SCO0727 (2H1)_PCR/7623 [Gene]SCO0727/6944 NC_003888 3SC5B7.05c, unknown, len: 307 aa 4 4 3 20 [Reporter]SCO4573 (3D1)_PCR/7622 [Gene]SCO4573/6943 NC_003888 SCD16A.10c, nuoL, NADH dehydrogenase subunit, len: 654 aa; similar to many e.g. TR:O86350 (EMBL:AL021646) NuoL, NADH dehydrogenase subunit from Mycobacterium tuberculosis (633 aa) fasta scores; opt: 2074, z-score: 2200.6, E(): 0, (61.1% identity in 648 aa overlap) and SW:NUOL_ECOLI, NADH dehydrogenase subunit NuoL from Escherichia coli (613 aa) fasta scores; opt: 1178, z-score: 1251.2, E(): 0, (38.2% identity in 659 aa overlap). Contains Pfam matches to entries PF00361 oxidored_q1, NADH-Ubiquinone/plastoquinone (complex I), various chains and Pfam match to entry PF00662 oxidored_q1_N, NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus. Contains possible membrane spanning hydrophobic regions. 4 4 3 19 [Reporter]SCO1399 (3P21)_PCR/7621 [Gene]SCO1399/6942 NC_003888 SC1A8A.19c, cvnD4, possible ATP/GTP-binding protein, len: 201aa; similar to others from Streptomyces coelicolor and Mycobacterium tuberculosis eg. TR:Q9XAE1 (EMBL:AL079356) putative ATP/GTP-binding protein from Streptomyces coelicolor (191 aa) fasta scores; opt: 742, z-score: 890.7, E(): 0, 60.9% identity in 184 aa overlap. Local genetic arrangement also seems to be conserved for the Streptomyces coelicolor paralogues. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 3 9 17 [Reporter]SCO6657 (6O2)_PCR/5232 [Gene]SCO6657/4769 NC_003888 SC5A7.07c, putative membrane protein, len: 527 aa; similar to two consecutive genes downstream of HISX_STRCO S. colelicolor histidinol dehydrogenase; TR:Q53931 and TR:Q53930, fasta scores; E(): 1.4e-07, 34.3% identity in 213 aa overlap and E(): 1.1e-06, 32.3% identity in 223 aa overlap. Contains possible hydrophobic membrane spanning region at C-terminal domain 4 4 3 18 [Reporter]SCO6227 (4L21)_PCR/7620 [Gene]SCO6227/6941 NC_003888 SC2H4.09, unknown, len: 517 aa; some similarity to Rhizobium sp. NGR234 plasmid pNGR234a hypothetical protein Y4XN_RHISN (628 aa), fasta scores; opt: 148 z-score: 254.8 E(): 6e-07, 25.9% identity in 495 aa overlap. Also weak similarity to S. coelicolor SC4H2.21 (E(): 1.8e-06, 33.2% identity in 581 aa overlap) and to divergent CDS SC2H4.08c (E(): 0.0012, 29.1% identity in 553 aa overlap) 3 3 9 16 [Reporter]SCO3604 (7K2)_PCR/5231 [Gene]SCO3604/4768 NC_003888 SC66T3.15c, possible membrane protein, len: 96 aa; unknown function, N-terminus similar to TR:O53503 (EMBL:L021957) Mycobacterium tuberculosis hypothetical protein (134 aa), fasta scores; opt: 134 z-score: 179.9 E(): 0.011, 34.7% identity in 101 aa overlap and TR:O69562 (EMBL:AL022602) Mycobacterium leprae hypothetical protein (134 aa) (38.3% identity in 60 aa overlap). Contains central hydrophobic region while the remainder is hydrophilic 4 4 3 16 [Reporter]SCO7579 (6D21)_PCR/7618 [Gene]SCO7579/6940 NC_003888 SC5F1.33, possible DNA-binding protein, len: 291 aa; similar to TR:Q9KZY5 (EMBL:AL353815) Streptomyces coelicolor putative DNA-binding protein SCD6.19, 295 aa; fasta scores: opt: 643 z-score: 787.2 E(): 0; 37.0% identity in 281 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix. Also contains possible helix-turn-helix motif at residues 31..52 (+6.28 SD) 3 3 9 15 [Reporter]SCO2154 (8G2)_PCR/5230 [Gene]SCO2154/4767 NC_003888 SC6G10.27c, possible integral membrane protein, len: 132aa; similar to proteins of undefined function eg. SW:Y00A_MYCTU hypothetical protein from Mycobacterium tuberculosis (139 aa) fasta scores; opt: 317, z-score: 403.3, E(): 3.7e-15, (38.3% identity in 133 aa overlap). 3 3 9 14 [Reporter]SCO7137 (9C2)_PCR/5229 [Gene]SCO7137/4766 NC_003888 SC8B1.01c, possible regulatory protein (fragment), len: >708 aa; similar to TR:Q9S1Z4 (EMBL:AL109747) Streptomyces coelicolor probable transcriptional regulator SCJ21.13, 919 aa; fasta scores: opt: 2764 z-score: 2921.5 E(): 0; 63.0% identity in 690 aa overlap and to TR:Q9ZIW5 (EMBL:AF009336) Streptomyces coelicolor putative transcriptional activator of lipA gene, LipR, 941 aa; fasta scores: opt: 332 z-score: 350.0 E(): 5e-12; 29.5% identity in 698 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Also contains a TTA leucine codon, possible target for bldA regulation,SC4B10.38c, probable transcriptional regulator (fragment), len: >249 aa; similar to C-terminal region of TR:Q9S1Z4 (EMBL:AL109747) Streptomyces coelicolor probable transcriptional regulator SCJ21.13, 919 aa; fasta scores: opt: 1004 z-score: 1188.9 E(): 0; 64.3% identity in 249 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and possible helix-turn-helix motif at residues 210..231 (+3.95 SD) 3 3 9 13 [Reporter]SCO1500 (9O22)_PCR/5228 [Gene]SCO1500/4765 NC_003888 SC9C5.24c, hypothetical protein, len: 167 aa; similar to SW:YP54_MYCTU (EMBL:Z83863) Mycobacterium tuberculosis hypothetical 18.0 kD protein MTCY159.02, 170 aa; fasta scores: opt: 461 z-score: 543.2 E(): 8.7e-23; 54.7% identity in 150 aa overlap 3 3 9 11 [Reporter]SCO4091 (11G22)_PCR/5226 [Gene]SCO4091/4764 NC_003888 SCD25.27, possible DNA-binding protein, len: 68 aa. Contains a possible helix-turn-helix motif between residues 13..34 score +3.25 SD. 3 3 9 10 [Reporter]SCO3384 (12C22)_PCR/5225 [Gene]SCO3384/4763 NC_003888 SCE126.02c, unknown, len: 420aa; similar to TR:O06279 (EMBL:Z95557) hypothetical protein from Mycobacterium tuberculosis (303 aa) fasta scores; opt: 683, z-score: 584.3, E(): 3.1e-25, (49.3% identity in 284 aa overlap). 3 3 9 9 [Reporter]SCO1690 (13O18)_PCR/5224 [Gene]SCO1690/4762 NC_003888 SCI30A.11, possible acyl-CoA dehydrogenase, len: 404 aa; varying similarity to many e.g. TR:P96831 (EMBL:Z92770) hypothetical protein from Mycobacterium tuberculosis (403 aa) fasta scores; opt: 1464, z-score: 1688.2, E(): 0, (57.3% identity in 400 aa overlap) and SW:ACDB_BACSU acyl-CoA dehydrogenase from Bacillus subtilis (379 aa) fasta scores; opt: 465, z-score: 539.6, E(): 1e-22, (32.0% identity in 347 aa overlap). Contains two Pfam matches to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase 2 2 15 14 [Reporter]SCO0003 (9J7)_PCR/2841 [Gene]SCO0003/2589 NC_003888 SC8E7.41c, possible DNA-binding protein, len: 783 aa. Contains a putative helix-turn-helix motif situated between residues 69..90 (+3.17 SD). 3 3 9 8 [Reporter]SCO3925 (14K18)_PCR/5223 [Gene]SCO3925/4761 NC_003888 SCQ11.08, probable transcriptional regulator, len: 241 aa; similar to many transcriptional regulators e.g. TR:Q43992 (EMBL:L05770), pobR, Acinetobacter calcoaceticus ADP1 transcriptional activator regulating expression of p-hydroxybenzoate hydroxylase (PobA) (271 aa), fasta scores; opt: 223 z-score: 262.9 E(): 2.6e-07, 25.9% identity in 239 aa overlap. Also similar to TR:O88025 (EMBL:AL031107) S.coelicolor probable transcriptional regulator (264 aa) (26.4% identity in 239 aa overlap). Contains probable helix-turn-helix motif at aa 19-40 (Score 1331, +3.72 SD) and possible hydrophobic membrane spanning region 2 2 15 13 [Reporter]SCO6218 (10F7)_PCR/2840 [Gene]SCO6218/2588 NC_003888 SC9G1.08c, possible phosphatase, len: 199 aa; similar to SW:COBC_SALTY (EMBL:U12808) Salmonella typhimurium alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.-) CobC, 234 aa; fasta scores: opt: 239 z-score: 291.8 E(): 7e-09; 31.8% identity in 201 aa overlap. Contains match to Pfam entry PF00300 PGAM, Phosphoglycerate mutase family 3 3 9 7 [Reporter]SCO3331 (15G18)_PCR/5222 [Gene]SCO3331/4760 NC_003888 SCE68.29c, possible transmembrane transport protein, len: 415 aa; similar to many e.g. TR:O30996 (EMBL:AF024666), cmx, Corynebacterium striatum R-plasmid pTP10 transposon Tn5564 chloramphenicol export protein (391 aa), fasta scores; opt: 215 z-score: 235.6 E(): 8.4e-06, 24.4% identity in 390 aa overlap. Similar to TR:O69867 (EMBL:AL023702), SC1C3.25, S.coelicolor putative transmembrane transport protein (410 aa) (32.1% identity in 383 aa overlap). Contains hydrophobic, possible membrane-spanning regions 2 1 21 2 [Reporter]SCO7473 (20N24)_PCR/945 [Gene]SCO7473/869 NC_003888 SCBAC17A6.06, possible phenylacetic acid degradation protein PaaC, len: 275 aa: similar to many e.g. SW:P76079 (PAAC_ECOLI) phenylacetic acid degradation protein PaaC from Escherichia coli (248 aa) fasta scores; opt: 470, Z-score: 547.8, 41.057% identity (44.105% ungapped) in 246 aa overlap. 2 2 15 12 [Reporter]SCO2536 (11B7)_PCR/2839 [Gene]SCO2536/2587 NC_003888 SCC77.03c, probable transmembrane transport protein, len: 499 aa. Highly similar to many including: Nocardia lactamdurans SW:CMCT_NOCLA(EMBL:Z13973) cephamycin export protein CmcT (486 aa), fasta scores opt: 721 z-score: 808.4 E():0 38.5% identity in 478 aa overlap and Streptomyces coelicolor TR:O54179(EMBL:AL021411) export protein SC7H1.20C (483 aa), fasta scores opt: 745 z-score: 835.2 E():0 47.1% identity in 495 aa overlap. Contains a Pfam match to entry PF00083 sugar_tr and multiple possible membrane spanning hydrophobic domains. 2 1 20 22 [Reporter]SCO1748 (1N20)_PCR/943 [Gene]SCO1748/868 NC_003888 2SCI34.01c, probable expression regulator (fragment), len: >159 aa; highly similar to metallothionein-like proteins e.g. TR:Q9WX50 (EMBL:AB012145) Streptomyces lividans MrnA encoding metallothionein-like domain exhibiting repressor function against protease expression, 315 aa; fasta scores: opt: 507 z-score: 589.0 E(): 2.3e-25; 98.8% identity in 81 aa overlap,SCI11.37c, probable expression regulator, partial CDS, len: >272 aa; highly similar to metallothionein-like proteins e.g. Streptomyces lividans CDS (EMBL:AB012145) exhibiting repressor function against protease expression (315 aa), fasta scores; opt: 1943 z-score: 1640.5 E(): 0, 97.8% identity in 272 aa overlap. The C-terminus contains a Pro-rich region (aa 170-200 approx.), followed by a Cys-rich region (aa 235-272 approx.). Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature 2 2 15 11 [Reporter]SCO4129 (12N3)_PCR/2838 [Gene]SCO4129/2586 NC_003888 SCD72A.15c, possible integral membrane protein, len: 420aa; contains possible membrane-spanning hydrophobic regions. 2 1 20 21 [Reporter]SCO4240 (2J20)_PCR/942 [Gene]SCO4240/867 NC_003888 SCD8A.13c, msiK, ABC transporter ATP-binding protein, len: 378 aa; highly similar to TR:P96483 (EMBL:Y08921) Streptomyces reticuli gene encoding MsiK protein and ORF1, 377 aa; fasta scores: opt: 2268 z-score: 2471.1 E(): 0; 92.6% identity in 378 aa overlap and to SW:UGPC_ECOLI (EMBL:X13141) Escherichia coli sn-glycerol-3-phosphate transport ATP-binding protein UgpC, 365 aa; fasta scores: opt: 1031 z-score: 1127.4 E(): 0; 48.8% identity in 379 aa overlap also similar to TR:CAB46805 (EMBL:AL096811) Streptomyces coelicolor SCI30A.28c, 445 aa; fasta scores: opt: 866 z-score: 719.0 E(): 1.4e-34; 49.3% identity in 292 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 2 2 15 10 [Reporter]SCO3040 (13J3)_PCR/2837 [Gene]SCO3040/2585 NC_003888 SCE34.21c, possible secreted protein, len: 416 aa; similar to TR:CAB51271 (EMBL:AL096872) Streptomyces coelicolor hypothetical 31.9 kD protein SC5F7.14c, 308 aa; fasta scores: opt: 646 z-score: 629.8 E(): 1.3e-27; 40.5% identity in 304 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and possible N-terminal region signal peptide sequence 2 1 20 20 [Reporter]SCO7376 (3F20)_PCR/941 [Gene]SCO7376/866 NC_003888 SC10G8.03c, unknown, len: 242 aa. Weakly similar to Aeropyrum pernix TR:Q9Y911 (EMBL:AP000064) hypothetical protein (205 aa), fasta scores opt: 226 z-score: 263.2 E(): 3e-07 31.8% identity in 211 aa overlap and to TR:CAC48481 (EMBL:AL603642) Rhizobium meliloti (Sinorhizobium meliloti) conserved hypothetical protein SMB20081, 236 aa; fasta scores: opt: 577 Z-score: 646.5 E(): 2.2e-28; 46.256% identity in 227 aa overlap 2 2 15 9 [Reporter]SCO3676 (14F3)_PCR/2836 [Gene]SCO3676/2584 NC_003888 SCH35.48c, possible integral membrane protein, len: 350aa; Contains possible membrane spanning hydrophobic regions in the C-terminal half and a hydrophobic N-terminal half. 2 1 20 19 [Reporter]SCO5620 (4B20)_PCR/940 [Gene]SCO5620/865 NC_003888 SC2E1.37, unknown prophage gene, len: 796 aa; shows very limited similarity to Rhodococcus opacus TR:G2935035 (EMBL:AF003948) putative transposase homolog (242 aa), fasta scores; opt: 215 z-score: 280.3 E(): 2.2e-08, 33.3% identity in 105 aa overlap; and similar to TR:O69933 (EMBL:AL023861) Streptomyces coelicolor putative DNA recombinase SC3C8.24, 672 aa; fasta scores: opt: 1276 Z-score: 1340.7 bits: 258.7 E(): 4.8e-67; 41.074% identity in 689 aa overlap. Contains PS00397 Site-specific recombinases active site and Pfam match to entry recombinase PF00239, Site-specific recombinases, score 36.16. The last part of this ORF (aa 672 onwards) appears to be a continuation of SC2E1.21, which was interrupted by the prophage; the similarities to YX27_MYCTU MTCY274.27c (389 aa) (fasta scores; opt: 246 z-score: 225.9 E(): 2.4e-05, 38.8% identity in 116 aa overlap) continue after aa 672. Contains probable coiled-coil from aa 449 to 505 2 2 15 8 [Reporter]SCO0136 (15B3)_PCR/2835 [Gene]SCO0136/2583 NC_003888 SCJ21.17c, probable integral membrane protein, len: 516 aa; similar to SGAT_ECOLI putative transport protein SGAT (484 aa), fasta scores; opt: 339 z-score: 341.6 E(): 1.1e-11, 28.5% identity in 414 aa overlap. Contains probable membrane spanning hydrophobic domains 2 1 20 18 [Reporter]SCO6692 (5N16)_PCR/939 [Gene]SCO6692/864 NC_003888 SC4C6.02c, probable integral membrane protein, len: 317 aa; similar to TR:O86668 (EMBL:AL031182) S.coelicolor probable integral membrane protein (299 aa), fasta scores; opt: 288 z-score: 279.1 E(): 3.2e-08, 32.8% identity in 299 aa overlap and to TR:O33234 (EMBL:Z98209) Mycobacterium tuberculosis hypothetical protein (301 aa) (28.8% identity in 299 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Weakly similar to TR:O86668 (EMBL:AL031182) S.coelicolor putative integral membrane protein (299 aa) (32.8% identity in 299 aa overlap) 2 2 15 7 [Reporter]SCO3803 (15N23)_PCR/2834 [Gene]SCO3803/2582 NC_003888 SCGD3.04c, hypothetical protein, len: 134 aa; shows similarity to TR:P96597 (EMBL:AB001488), YdbB, Bacillus subtilis hypothetical protein (113 aa), fasta scores; E(): 2.8e-05, 35.6% identity in 87 aa overlap and to hypothetical proteins from Neisseria meningitidis serogroup A, e.g. NMA0576, hypothetical protein (107 aa), blastp scores: Expect= 4.6e-11. Probable member of the cupin superfamily 2 1 20 17 [Reporter]SCO2182 (6J16)_PCR/938 [Gene]SCO2182/863 NC_003888 SC5F7.19, possible gntR-family transcriptional regulator, len: 207aa; similar to many egs. SW:GNTR_BACSU gluconate operon transcriptional repressor (243 aa) fasta scores; opt: 192, z-score: 240.4, E(): 4.7e-06, (31.1% identity in 180 aa overlap) and TR:CAA22369 (EMBL:AL034443) putative transcriptional regulator from Streptomyces coelicolor (225 aa) fasta scores; opt: 242, z-score: 300.9, E(): 2e-09, (32.2% identity in 199 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. 2 2 15 6 [Reporter]SCO6663 (16J23)_PCR/2833 [Gene]SCO6663/2581 NC_003888 SC5A7.13c, tktB, probable transketolase, len: 698 aa; highly similar to many e.g. TKT2_ECOLI transketolase 2 (EC 2.2.1.1) (667 aa), fasta scores; opt: 1191 z-score: 1912.7 E(): 0, 46.0% identity in 683 aa overlap. Contains PS00802 Transketolase signature 2 and Pfam match to entry PF00456 transketolase, Transketolase, score 1163.10, E-value 0 2 1 20 16 [Reporter]SCO6180 (7F16)_PCR/937 [Gene]SCO6180/862 NC_003888 SC2G5.01, partial CDS, possible transferase, len:>187aa; constitutes the C-terminal end of SC6C5.16 (EMBL:AL034 492) from Streptomyces coelicolor. Similar to the C-terminal region of many egs. SW:CMCH_NOCLA3'-hydroxymethylcephem- O-carbamoyltransferase, a protein involved in cephamycin synthesis in Nocardia lactamdurans(520 aa) fasta scores; opt : 373, z-score: 451.4, E():7.4e-18, (40.1% identity in 167 aa overlap).,SC6C5.16, partial CDS, unknown, len: 392aa; similar to many eg. SW:NOLO_RHISN nodulation associated protein NolNO from Rhizobium sp. (strain NGR234) (680 aa) fasta scores; opt: 696, z-score: 849.5, E(): 0, (36.7% identity in 403 aa overlap). 2 2 15 5 [Reporter]SCO1194 (17F23)_PCR/2832 [Gene]SCO1194/2580 NC_003888 SCG11A.25, probable export protein, len: 496 aa; similar to TR:P72184 (EMBL:U51164) Propionibacterium freudenreichii membrane-bound transport protein HemX, 487 aa; fasta scores: opt: 1371 z-score: 1416.9 E(): 0; 47.0% identity in 474 aa overlap and to SW:PUR8_STRLP (EMBL:X76855) Streptomyces lipmanii (Streptomyces alboniger) puromycin resistance protein Pur8, 503aa; fasta scores: opt: 576 z-score: 598.4 E(): 5.9e-26; 30.2% identity in 493 aa overlap 2 1 20 15 [Reporter]SCO5236 (8B16)_PCR/936 [Gene]SCO5236/861 NC_003888 SC7E4.33c, nagB, probable glucosamine phosphate isomerase, len: 261 aa; similar to SW:NAGB_ECOLI (EMBL:M19284) Escherichia coli glucosamine-6-phosphate isomerase (EC 5.3.1.10) NagB, 266 aa; fasta scores: opt: 630 z-score: 704.6 E(): 8.9e-32; 44.6% identity in 233 aa overlap. Contains Pfam match to entry PF01182 Glucosamine_iso, Glucosamine-6-phosphate isomerase 2 1 20 14 [Reporter]SCO5407 (9N12)_PCR/935 [Gene]SCO5407/860 NC_003888 SC8F4.11c, hypothetical protein, len: 219 aa; weakly similar to many e.g. TR:AAF10203 (EMBL:AE00192) hypothetical protein from Deinococcus radiodurans (254 aa) fasta scores; opt: 388, z-score: 453.5, E(): 7.5e-18, 36.9% identity in 214 aa overlap. Also weakly similar to the neighbouring hypothetical CDSs SC8F4.10c and SC8F4.12c 4 4 3 15 [Reporter]SCO2126 (7P17)_PCR/7617 [Gene]SCO2126/6939 NC_003888 SC6E10.20c, glk, glucokinase, len: 317 aa; previously sequenced as SW:GLK_STRCO (EMBL:X65932), Glk, Streptomyces coelicolor glucokinase (317 aa), identical to this sequence. Similar to other ROK-family proteins from S.coelicolor e.g. TR:O69941 (EMBL:AL023862) S. coelicolor hypothetical ROK family protein (403 aa) (34.7% identity in 317 aa overlap). Pfam match to entry PF00480 ROK, ROK family and PS01125 ROK family signature 4 4 3 14 [Reporter]SCO2017 (8L17)_PCR/7616 [Gene]SCO2017/6938 NC_003888 SC7H2.31c, possible amino acid decarboxylase, len: 452aa; similar to many eg. TR:Q43908 (EMBL:D55724) L-2,4-diaminobutyrate decarboxylase of Acinetobacter baumannii (510 aa) fasta scores; opt: 532, z-score: 544.9, E(): 5e-23, (31.8% identity in 431 aa overlap). Contains Pfam match to entry PF00282 pyridoxal_deC, Pyridoxal-dependent decarboxylase conserved domain. 4 4 3 13 [Reporter]SCO7140 (9H17)_PCR/7615 [Gene]SCO7140/6937 NC_003888 SC9A4.02, probable DNA-binding protein, len: 276 aa; highly similar to TR:Q9S1Q8 (EMBL:AL109972) Streptomyces coelicolor putative DNA-binding protein SCJ9A.12c, 279 aa; fasta scores: opt: 1046 z-score: 1203.5 E(): 0; 59.1% identity in 274 aa overlap. Contains a possible helix-turn-helix motif at residues 36..57 (+3.89 SD) 4 4 3 11 [Reporter]SCO4495 (11P13)_PCR/7613 [Gene]SCO4495/6936 NC_003888 SCD35.02, possible DNA polymerase related protein, len: 212 aa; similar to TR:AAF11304 (EMBL:AE002016) Deinococcus radiodurans DNA polymerase-related protein DR1751, 237 aa; fasta scores: opt: 380 z-score: 451.4 E(): 1e-1; 41.5% identity in 188 aa overlap and to N-terminal region of SW:DPOL_BPSP1 (EMBL:M84415) Bacteriophage SP01 DNA polymerase (EC 2.7.7.7) 31, 924 aa; fasta scores: opt: 191 z-score: 223.4 E(): 5.2e-05; 33.7% identity in 169 aa overlap 4 4 3 10 [Reporter]SCO3515 (12L13)_PCR/7612 [Gene]SCO3515/6935 NC_003888 SCE134.16, possible integral membrane protein, len: 488aa; contains a possible membrane spanning hydrophobic region. 4 4 3 9 [Reporter]SCO1049 (13H13)_PCR/7611 [Gene]SCO1049/6934 NC_003888 SCG20A.29c, probable secreted oxidoreductase, len: 387 aa; similar to SW:GFO_ZYMMO (EMBL:M97379) Zymomonas mobilis glucose-fructose oxidoreductase precursor (EC 1.1.99.28) Gfo, 439 aa; fasta scores: opt: 287 z-score: 318.4 E(): 2.9e-10; 26.6% identity in 350 aa overlap. Contains Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family and possible N-terminal region signal peptide sequence 4 4 3 8 [Reporter]SCO0862 (14D13)_PCR/7610 [Gene]SCO0862/6933 NC_003888 SCM2.15c, possible integral membrane protein, len: 219 aa. Similar to a protein of unknown function from Mycobacterium tuberculosis SW:Y970_MYCTU(EMBL:Z79700) hypothetical 22.9 KD protein (210 aa), fasta scores opt: 224 z-score: 235.4 E(): 9.8e-06 30.5% identity in 213 aa overlap. Contains possible membrane spanning hydrophobic domains and a a possible N-terminal signal sequence. 4 4 3 7 [Reporter]SCO5054 (15P9)_PCR/7609 [Gene]SCO5054/6932 NC_003888 SCK7.27, unknown, len: 104 aa 3 3 9 6 [Reporter]SCO3150 (16C18)_PCR/5221 [Gene]SCO3150/4759 NC_003888 SCE87.01c, unknown, partial CDS, len: > 329 aa. Similar in parts to Mycobacterium tuberculosis TR:O05594 (EMBL:Z94752) hypothetical 38.1 KD protein (362 aa), fasta scores opt: 365 z-score: 372.3 E(): 2.3e-13 34.1% identity in 232 aa overlap and Bacillus subtilis SW:YABE_BACSU (EMBL:D26185) hypothetical 47.7 KD protein (437 aa), fasta scores opt: 315 z-score: 321.5 E(): 1.5e-10 29.2% identity in 233 aa overlap.,SCE66.29c, unknown, partial CDS, len: >165aa; similar to TR:O05594 (EMBL:Z94752) hypothetical protein from Mycobacterium tuberculosis (362 aa) fasta scores; opt: 316, z-score: 379.7, E(): 1.1e-13, 37.8% identity in 156 aa overlap. 4 4 3 6 [Reporter]SCO2955 (16L9)_PCR/7608 [Gene]SCO2955/6931 NC_003888 SCE59.14c, hypothetical protein, len: 196 aa; similar to various hypothetical proteins, e.g. TR:CAB56723 (EMBL:AL121600) Streptomyces coelicolor hypothetical 22.2kD protein SCF76.03, 204 aa; fasta scores: opt: 357 z-score: 403.5 E(): 4.6e-15; 37.4% identity in 187 aa overlap 3 3 9 5 [Reporter]SCO3368 (17O14)_PCR/5220 [Gene]SCO3368/4758 NC_003888 SCE94.19c, possible peptidase (putative secreted protein), len: 228 aa; similar to SW:ALE1_STACP glycylglycine endopeptidase from Staphylococcus capitis EPK1 (362 aa) fasta scores; opt: 329, z-score: 324.3, E(): 9.5e-11, (38.9% identity in 180 aa overlap). Contains possible N-terminal region signal peptide sequence 4 4 3 5 [Reporter]SCO3804 (17H9)_PCR/7607 [Gene]SCO3804/6930 NC_003888 SCGD3.05, probable araC-family transcriptional regulator, len: 334 aa; probable araC-type regulator similar to many e.g. TR:O30507 (EMBL:AF008308), ArgR, Pseudomonas aeruginosa arginine regulatory protein (328 aa), fasta scores; opt: 398 z-score: 463.6 E(): 1.7e-18, 30.8% identity in 334 aa overlap. Similar to others from S.coelicolor e.g. (EMBL:AL049587), SC5F2A.29, S.coelicolor possible transcriptional regulator (325 aa) (37.6% identity in 314 aa overlap). Contains probable helix-turn-helix motif at aa 240-261 (Score 1477, +4.22 SD). A second, less probable motif is at aa 305-326 (Score 1090, +2.90 SD). Contains Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family 3 3 9 4 [Reporter]SCP1.230 (18K14)_PCR/5219 [Gene]SCP1.230/4757 NC_003888 SCP1.230, mmyB, possible DNA-binding protein, len: 313aa; previously sequenced and annotated as TR:Q9JN77 (EMBL:AJ276673). Similar to many from Streptomyces coelicolor eg. TR:Q9RDF4 (EMBL:AL136503) putative DNA-binding protein (294 aa) fasta scores; opt: 670, z-score: 810.9, E(): 0, 43.1% identity in 255 aa overlap. Contains helix-turn-helix motif at residue 52-73 (Score 1329 (+3.71 SD)). Contains a TTA encoded leucine at residue 23, possible target for bldA regulation. 3 3 9 3 [Reporter]SCO1868 (19G14)_PCR/5218 [Gene]SCO1868/4756 NC_003888 SCI39.15c, conserved hypothetical protein, len: 364 aa; similar to SW:YCBU_BACSU (EMBL:AB000617) Bacillus subtilis hypothetical protein homologue of class-V proteins of pyridoxal-phosphate-dependent aminotransferase YcbU, aa; fasta scores: opt: 277 Z-score: 303.9 bits: 64.9 E(): 2.5e-09; 27.707% identity in 314 aa overlap 3 3 9 2 [Reporter]SCO3221 (20O10)_PCR/5217 [Gene]SCO3221/4755 NC_003888 SCE8.14c, probable oxidoreductase, len: 284 aa; similar to many e.g. TR:O52817 (EMBL:AJ223999) protein similar to prephenate dehydrogenase from the vancomycin biosynthesis of Amycolatopsis orientalis (367 aa) fasta scores; opt: 757, z-score: 865.8, E(): 0, (49.8% identity in 283 aa overlap) and SW:TYRA_BACSU prephenate dehydrogenase from Bacillus subtilis (372 aa) fasta scores; opt: 251, z-score: 291.1, E(): 6.6e-09, (28.3% identity in 251 aa overlap). 3 3 8 22 [Reporter]SCO4219 (1O6)_PCR/5215 [Gene]SCO4219/4754 NC_003888 2SCD46.33, possible hydrolase (dienelactone hydrolase family), len: 254aa; similar to many eg. SW:P27136 (TFE1_ALCEU) carboxymethylenebutenolidase I (EC 3.1.1.45) from Alcaligenes eutrophus (Ralstonia eutropha) (234 aa) fasta scores; opt: 215, z-score: 250.7, E(): 1.7e-06, 28.3% identity in 230 aa overlap and TR:CAB92202 (EMBL:AL356592) putative hydrolase from Streptomyces coelicolor (251 aa) fasta scores; opt: 754, z-score: 853.2, E(): 0, 48.4% identity in 248 aa overlap. Contains Pfam match to entry PF01738 DLH, Dienelactone hydrolase family. 3 3 8 21 [Reporter]SCO7526 (2K6)_PCR/5214 [Gene]SCO7526/4753 NC_003888 SC8G12.02, cmlR1, chloramphenicol resistance protein, len: 392 aa. Highly similar to many including: Streptomyces lividans SW:CMLR_STRLI(EMBL:X59968) chloramphenicol resistance protein, CmlR (392 aa), fasta scores opt: 940 z-score: 1039.3 E():0 40.6% identity in 397 aa overlap and Streptomyces violaceus TR:Q56147(EMBL:U09991) chloramphenicol resistance protein, CmlV (436 aa), fasta scores opt: 2023 z-score: 2227.6 E():0 80.0% identity in 404 aa overlap. Also similar to several Streptomyces coelicolor efflux protein e.g. TR:CAB66188(EMBL:AL136500) SC1G2.16C (413 aa), fasta scores opt: 1036 z-score: 1144.4 E():0 41.6% identity in 406 aa overlap. Contains a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter. Contains multiple possible membrane spanning hydrophobic domains. 3 3 8 19 [Reporter]SCO6929 (4C6)_PCR/5212 [Gene]SCO6929/4752 NC_003888 SC1G8.01c, unknown, partial CDS,len: >270 aa. Weakly similar to Bacillus subtilis SW:SPAC_BACSU (EMBL:M86869) subtilin biosynthetic protein, possibly involved in export, SpaC (441 aa), fasta scores opt: 319 z-score: 378.0 E(): 1.4e-13 29.0% identity in 245 aa overlap.,SC1B2.35c, unknown, partial CDS, len: >181 aa. Similar to Streptomyces coelicolor TR:CAB55532 (EMBL:AL117322) hypothetical 49.0 kd protein, SCF1.12 (466 aa), fasta scores opt: 238 z-score: 283.2 E(): 2.6e-08 31.1% identity in 180 aa overlap. 2 2 15 4 [Reporter]SCO0058 (18B23)_PCR/2831 [Gene]SCO0058/2579 NC_003888 SCJ4.39, unknown, len: 240 aa 3 3 8 17 [Reporter]SCO6653 (6K2)_PCR/5210 [Gene]SCO6653/4751 NC_003888 SC5A7.03, probable integral membrane protein, len: 400 aa. Note 63 aa overlap with convergent downstream ORF. Contains possible hydrophobic membrane spanning regions 2 2 15 3 [Reporter]SCO1980 (19N19)_PCR/2830 [Gene]SCO1980/2578 NC_003888 SC3C9.15, hypothetical protein, len: 237 aa; similar to TR:O86675 (EMBL:AL031371) Streptomyces coelicolor hypothetical 21.4 kDa protein SC4G2.02c, 196 aa; fasta scores: opt: 248 Z-score: 277.4 bits: 58.4 E(): 7.7e-08; 34.239% identity in 184 aa overlap. Contains Pfam match to entry PF02518 HATPase_c, Histidine kinase-, DNA gyrase B-, phytochrome-like ATPase and a TTA leucine codon, possible target for bldA regulation 3 3 8 16 [Reporter]SCO0308 (7G2)_PCR/5209 [Gene]SCO0308/4750 NC_003888 SC5G9.17, possible membrane protein, len: 467 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains hydrophobic, possible membrane-spanning regions near the C-terminus. Contains TTA (leucine) codon; possible target for bldA regulation 2 1 20 13 [Reporter]SCO2586 (10J12)_PCR/934 [Gene]SCO2586/859 NC_003888 SCC123.24c, unknown, len: 176 aa. 2 2 15 2 [Reporter]SCO3990 (20F19)_PCR/2829 [Gene]SCO3990/2577 NC_003888 SCBAC25E3.27c, unknown, len: 89 aa: no significant database matches. 2 1 20 12 [Reporter]SCO2616 (11F12)_PCR/933 [Gene]SCO2616/858 NC_003888 SCC80.01, unknown (fragment), len: >142 aa,SCC88.27, possible membrane protein, len: >225 aa. Rich in proline and glycine. Contains possible hydrophobic membrane spanning region and the following repeats at the N-terminal region: 4x(QPGPYG) and 4x(PPYGQQ) 2 2 14 22 [Reporter]SCO1002 (1F15)_PCR/2827 [Gene]SCO1002/2576 NC_003888 2SCG2.15c, unknown, len: 128 aa 2 1 20 11 [Reporter]SCO4182 (12B12)_PCR/932 [Gene]SCO4182/857 NC_003888 SCD66.19c, conserved hypothetical protein, len: 141 aa; highly similar to TR:Q54235 (EMBL:D63706) Streptomyces griseus hypothetical protein ORF2, 149 aa; fasta scores: opt: 766 z-score: 952.7 E(): 0; 81.9% identity in 149 aa overlap 2 2 14 21 [Reporter]SCO4828 (2B15)_PCR/2826 [Gene]SCO4828/2575 NC_003888 SC2A6.13, gbsA, betaine aldehyde dehydrogenase, len: 509 aa; highly similar to SW:DHAB_BACSU (EMBL:U47861) Bacillus subtilis betaine aldehyde dehydrogenase (EC 1.2.1.8) GsbA, 490 aa; fasta scores: opt: 1874 z-score: 2114.6 E(): 0; 56.0% identity in 495 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family and matches to Prosite entries PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site 2 1 20 10 [Reporter]SCO3099 (13N8)_PCR/931 [Gene]SCO3099/856 NC_003888 SCE41.08c, probable cytochrome P450 hydroxylase, len: 433 aa; similar to SW:CPXR_BRAJA (EMBL:U12678) Bradyrhizobium japonicum cytochrome P450 BJ-3 (EC 1.14.14.-) CYP114, 429 aa; fasta scores: opt: 682 z-score: 792.5 E(): 0; 32.9% identity in 435 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450 2 2 14 20 [Reporter]SCO5919 (3N11)_PCR/2825 [Gene]SCO5919/2574 NC_003888 SC10A5.24c, unknown, len: 134 aa 2 1 20 9 [Reporter]SCO0120 (14J8)_PCR/930 [Gene]SCO0120/855 NC_003888 SCJ21.01c, probable integral membrane protein, partial CDS, len: >114 aa,SCJ11.49c, probable integral membrane protein, len: >222aa; contains probable membrane spanning hydrophobic regions. 2 2 14 19 [Reporter]SCO7018 (4J11)_PCR/2824 [Gene]SCO7018/2573 NC_003888 SC1H10.07, unknown, len: 505 aa. 2 1 20 8 [Reporter]SCO0407 (15F8)_PCR/929 [Gene]SCO0407/854 NC_003888 SCF51.06, possible transcriptional regulator, len: 334 aa; similar to SW:TIPA_STRLI (EMBL:S64314) Streptomyces lividans transcriptional activator TipA, 253 aa; fasta scores: opt: 215 z-score: 237.9 E(): 7e-06; 37.9% identity in 103 aa overlap and to TR:O54191 (EMBL:AL021411) Streptomyces coelicolor putative transcriptional regulator SC7H1.32c, len: 196 aa; fasta scores: opt: 264 z-score: 290.9 E(): 7.8e-09; 37.4% identity in 139 aa overlap. Contains a Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and a prosite match to entry PS00552 Bacterial regulatory proteins, merR family signature. Contains possible helix-turn-helix motif between residues 3351..3416, score 1170 (+3.17 SD) 2 2 14 18 [Reporter]SCO5454 (5F11)_PCR/2823 [Gene]SCO5454/2572 NC_003888 SC3D11.11, possible two-component system sensor kinase, len: 442 aa; similar to many e.g. TR:CAA74719 (EMBL:Y14336) histidine autokinase from Streptomyces reticuli (398 aa) fasta scores; opt: 519, z-score: 573.4, E(): 1.6e-24, 36.3% identity in 369 aa overlap and SW:M23558 (DEGS_BACSU) sensor protein DegS from Bacillus subtilis (385 aa) fasta scores; opt: 343, z-score: 381.0, E(): 8.2e-14, 31.7% identity in 221 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 20 7 [Reporter]SCO0059 (16B8)_PCR/928 [Gene]SCO0059/853 NC_003888 SCJ4.40, possible zinc-binding oxidoreductase, len: 268 aa; similar to many both prokaryote and eukaryote egs. TR:O85702 (EMBL:AF072709) putative oxidoreductase from Streptomyces lividans 66 (313 aa) fasta scores; opt: 494, z-score: 509.6, E(): 5e-21, (41.3% identity in 240 aa overlap) and SW:QOR_CAVPO quinone oxidoreductase from Cavia porcellus (guinea pig) (329 aa) fasta scores; opt: 397, z-score: 411.5, E(): 1.5e-15, (32.8% identity in 244 aa overlap). Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases 2 2 14 17 [Reporter]SCO5796 (6B11)_PCR/2822 [Gene]SCO5796/2571 NC_003888 SC4H2.17, unknown, len: 497 aa; similar to hypothetical proteins from many organisms e.g. HFLX_ECOLI P25519 GTP-binding protein HflX (426 aa), fasta scores; opt: 904 z-score: 715.0 E(): 1.3e-32, 39.6% identity in 407 aa overlap.Contains PS00017 ATP/GTP-binding site motif A (P-loop). Contains probable coiled-coil from aa 235 to 271 2 1 20 6 [Reporter]SCO1769 (17N4)_PCR/927 [Gene]SCO1769/852 NC_003888 SCI51.09c, conserved hypothetical protein, len: 223 aa; unknown function, similar to hypothetical proteins from Mycobacterium leprae and Mycobacterium tuberculosis e.g. TR:O05669 (EMBL:Z95117) Mycobacterium leprae hypothetical protein (231 aa), fasta scores; opt: 633 z-score: 766.9 E(): 0, 54.5% identity in 187 aa overlap 2 2 14 16 [Reporter]SCO6763 (7N7)_PCR/2821 [Gene]SCO6763/2570 NC_003888 SC6A5.12, probable polyprenyl synthatase, len: 378aa; similar to many eg. SW:GGPP_MYCTU probable geranylgeranyl pyrophosphate synthatase from Mycobacterium tuberculosis (359 aa) fasta scores; opt: 1103, z-score: 1231.0, E(): 0, (49.3% identity in 355 aa overlap) and SW:IDSA_METTM short chain isoprenyl diphosphate synthase from Methanobacterium thermoautotrophicum (324 aa) fasta scores; opt: 547, z-score: 614.0, E(): 6.7e-27, (38.2% identity in 293 aa overlap). Contains Pfam match to entry PF00348 polyprenyl_synt, Polyprenyl synthetases, score 176.70, E-value 3.9e-49 and Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop), PS00723 Polyprenyl synthetases signature 1 and PS00606 Beta-ketoacyl synthases active site. 2 1 20 5 [Reporter]SCO0371 (18J4)_PCR/926 [Gene]SCO0371/851 NC_003888 SCF41.30c, possible secreted protein, len: 900 aa. Shares a low level of similarity, over the available sequence, with Bacteroides ovatus TR:Q45327 (EMBL; U15179) arabinosidase (ASDII) (fragment) (>436 aa), fasta scores opt: 323 z-score: 317.9 E(): 2.4e-10 30.6% identity in 304 aa overlap. Contains a possible N-terminal signal sequence. 2 1 20 4 [Reporter]SCO1294 (19F4)_PCR/925 [Gene]SCO1294/850 NC_003888 SCBAC36F5.05c, possible cystathionine gamma-synthase, len: 407 aa; similar to SW:METB_HAEIN (EMBL:U32694) Haemophilus influenzae cystathionine gamma-synthase (EC 4.2.99.9) MetB or HI0086, 369 aa; fasta scores: opt: 625 Z-score: 685.3 bits: 135.6 E(): 1.5e-30; 34.127% identity in 378 aa overlap. Contains Pfam match to entry PF01053 Cys_Met_Meta_PP, Cys/Met metabolism PLP-dependent enzyme and match to Prosite entry PS01050 Uncharacterized protein family UPF0031 signature 2 4 4 3 4 [Reporter]SCO5004 (18D9)_PCR/7606 [Gene]SCO5004/6929 NC_003888 SCK15.06, unknown, len: 313 aa. Contains hydrohilic C-terminall region 4 4 3 3 [Reporter]SCP1.282 (19P5)_PCR/7605 [Gene]SCP1.282/6928 NC_003888 SCP1.282, unknown, len: 83aa; 4 4 3 2 [Reporter]SCO1986 (20H5)_PCR/7604 [Gene]SCO1986/6927 NC_003888 SC3C9.21c, hypothetical protein, len: 260 aa; low similarity to TR:O54361 (EMBL:U73025) Staphylococcus aureus transposon TN5405 ORFX (hypothetical 32.9 kDa protein), 289 aa; fasta scores: opt: 241 Z-score: 283.5 bits: 60.2 E(): 3.5e-08; 28.251% identity in 223 aa overlap 4 4 2 22 [Reporter]SCO5184 (1H1)_PCR/7602 [Gene]SCO5184/6926 NC_003888 2SC3B6.08, possible ATP-dependent DNA helicase, len: 1222 aa; similar to TR::O53347 (EMBL:AL021646) Mycobacterium tuberculosis putative ATP-dependent DNA helicase, MTV014.45c, 1101 aa; fasta scores: opt: 1209 z-score: 1062.9 E(): 0; 38.4% identity in 1199 aa overlap to SW:HELD_ECOLI (EMBL:J04726) Escherichia coli helicase IV (EC 3.6.1.-) HelD, 648 aa; fasta scores: opt: 216 z-score: 194.6 E(): 0.0023; 25.3% identity in 471 aa overlap and to Streptomyces coelicolor 2SC3B6.07, 1159 aa; fasta scores: opt: 406 z-score: 293.0 E(): 1e-10; 30.2% identity in 977 aa overlap. Contains Pfam match to entry PF00580 UvrD-helicase, UvrD/REP helicase and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 4 2 21 [Reporter]SCO4912 (2D1)_PCR/7601 [Gene]SCO4912/6925 NC_003888 SCK13.04c, possible aldehyde dehydrogenase, len: 293 aa; similar to C-terminal region of many eukaryotic aldehyde dehydrogenases, e.g. TR:Q9LLR2 (EMBL:AF162665) Oryza sativa aldehyde dehydrogenase AldH, 549 aa; fasta scores: opt: 445 z-score: 486.0 E(): 1.6e-19; 36.3% identity in 248 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family 4 4 2 20 [Reporter]SCO6532 (2P21)_PCR/7600 [Gene]SCO6532/6924 NC_003888 SC5C7.17c, narI, probable nitrate reductase gamma chain; len: 240 aa; similar to many e.g. NARI_BACSU nitrate reductase gamma chain (EC 1.7.99.4) (223 aa), fasta scores; opt: 664 z-score: 1084.3 E(): 0, 41.5% identity in 217 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 2 19 [Reporter]SCO1394 (3L21)_PCR/7599 [Gene]SCO1394/6923 NC_003888 SC1A8A.14, possible glycosyl hydrolase, len: 795 aa; similar to many e.g. SW:O59645 (AGLU_SULSO) alpha-glucosidase from Sulfolobus solfataricus (693 aa) fasta scores; opt: 535, z-score: 568.6, E(): 3e-24, 32.4% identity in 463 aa overlap. Contains Pfam match to entry PF01055 Glyco_hydro_31, Glycosyl hydrolases family 31. 4 4 2 18 [Reporter]SCO6182 (4H21)_PCR/7598 [Gene]SCO6182/6922 NC_003888 SC2G5.03, possible dehydratase, len: 285aa; N-terminal region similar to SW:HIS7_SALTY, HisB, imidazoleglycerol-phosphate dehydratase (354 aa) from Salmonella typhimurium fasta scores; opt: 207, z-score:191.1, E(): 0.0023, (29.4 %identity in 143 aa overlap). Also similar TR:Q54364 (EMBL:X79146) located within thelincomycin-production gene cluster of Streptomyces lincolnensis 78-11 (190 aa) fasta scores opt: 345, z-score:312.0, E(): 4.3e-10, (40.5% identity in 185 aa overlap). 3 3 8 15 [Reporter]SCO2150 (8C2)_PCR/5208 [Gene]SCO2150/4749 NC_003888 SC6G10.23c, qcrC, cytochrome C heme-binding subunit, len: aa; similar to many eg. TR:O69583 (EMBL:AL022602) proposed QcrC protein from Mycobacterium leprae (164 aa) fasta scores; opt: 368, z-score: 374.3, E(): 1.5e-13, (45.4% identity in 141 aa overlap). Contains two Pfam matches to entry PF00034 cytochrome_c, Cytochrome c and two Prosite matches to PS00190 Cytochrome c family heme-binding site signature. Also contains possible membrane spanning hydrophobic regions. 4 4 2 17 [Reporter]SCO7118 (5D21)_PCR/7597 [Gene]SCO7118/6921 NC_003888 SC4B10.19c, unknown, len: 254 aa 3 3 8 14 [Reporter]SCO2413 (8O22)_PCR/5207 [Gene]SCO2413/4748 NC_003888 SC8A2.01c, possible secreted protein (fragment), len: >42 aa. Contains possible N-terminal region signal peptide sequence,SC4A7.41c, possible membrane protein (partial CDS), len: >165 aa; similar to various hypothetical proteins, e.g. TR:P71754 (EMBL:Z81368) Mycobacterium tuberculosis hypothetical 19.9 kD protein MCTY253.31, 185 aa; fasta scores: opt: 125 z-score: 158.3 E(): 0.2; 26.0% identity in 150 aa overlap and to S. coelicolor SC4A7.39c, 192 aa; fasta scores: opt: 240 z-score: 236.5 E(): 8.8e-08; 36.0% identity in 161 aa overlap 4 4 2 16 [Reporter]SCO2168 (6P17)_PCR/7596 [Gene]SCO2168/6920 NC_003888 SC5F7.33c, unknown, possible hydrophilic protein, len: 259aa; some similartity to others of undefined function eg. SW:Y617_SYNY3 hypothetical protein from Synechocystis sp. strain PCC6803 (267 aa) fasta scores; opt: 313, z-score: 322.8, E(): 1.2e-10, (30.6% identity in 232 aa overlap). Contains probable coiled coil regions approx. 35-55aa and 45-110aa. 3 3 8 13 [Reporter]SCO1492 (9K22)_PCR/5206 [Gene]SCO1492/4747 NC_003888 SC9C5.16c, possible peptidase, len: 368 aa; similar to TR:Q9X842 (EMBL:AL049727) Streptomyces coelicolor putative dipeptidase SC9B1.23, 376 aa; fasta scores: opt: 552 z-score: 631.8 E(): 1e-27; 33.8% identity in 370 aa overlap. Contains Pfam match to entry PF00557 Peptidase_M24, metallopeptidase family M24 3 3 8 12 [Reporter]SCO2718 (10G22)_PCR/5205 [Gene]SCO2718/4746 NC_003888 SCC46.03c, putative secreted protein, len: 131 aa; similar to S. coelicolor unknown protein SCC46.04, 147 aa; fasta scores: opt: 626 z-score: 635.3 E(): 6.2e-30; 69.2% identity in 130 aa overlap. Contains possible N-terminal region signal peptide sequence 3 3 8 11 [Reporter]SCO4084 (11C22)_PCR/5204 [Gene]SCO4084/4745 NC_003888 SCD25.20, unknown, len: 437 aa. Similar in parts to Mycobacterium tuberculosis TR: O06627 (EMBL; Z95618) hypothetical 13.5 KD protein MTCY07H7A.02C (129 aa),fasta scores opt: 333 z-score: 391.1 E(): 2e-14 54.0% identity in 100 aa overlap and Mycobacterium leprae TR:O05761 (EMBL; Z95151) hypothetical 14.0 KD protein MLCB5.37 (131 aa), fasta scores opt: 328 z-score: 385.3 E(): 4.1e-14 52.5% identity in 101 aa overlap. 3 3 8 10 [Reporter]SCO2922 (12O18)_PCR/5203 [Gene]SCO2922/4744 NC_003888 SCE19A.22c, possible membrane protein, len: 148 aa; unknown function, weakly similar to TR:O34424 (EMBL:AF008220) Bacillus subtilis hypothetical protein (164 aa), fasta scores; opt: 137 z-score: 190.5 E(): 0.0028, 30.4% identity in 135 aa overlap. Weak similarity to adjacent CDS SCE19.23c, hypothetical protein (224 aa) (29.1% identity in 158 aa overlap). Contains a hydrophobic, possible membrane-spanning region 3 3 8 9 [Reporter]SCO3629 (13K18)_PCR/5202 [Gene]SCO3629/4743 NC_003888 SCH10.07c, purA, probable adenylosuccinate synthetase, len: 426 aa; similar to many e.g. SW:PURA_ECOLI (EMBL:J04199), PurA, Escherichia coli adenylosuccinate synthetase (431 aa), fasta scores; opt: 1421 z-score: 1608.0 E(): 0, 50.5% identity in 420 aa overlap. Contains Pfam match to entry PF00709 Adenylsucc_synt, Adenylosuccinate synthetase, score 742.40, E-value 1.9e-219, PS00513 Adenylosuccinate synthetase active site and PS01266 Adenylosuccinate synthetase GTP-binding site 3 3 8 8 [Reporter]SCO0825 (14G18)_PCR/5201 [Gene]SCO0825/4742 NC_003888 SCF43A.15, possible transmembrane transport protein, len: 318 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains hydrophobic, possible membrane-spanning regions. May be involved in membrane transport in association with its upstream neighbour which resembles an ABC-tranporter ATP-binding protein 2 2 14 15 [Reporter]SCO2473 (8J7)_PCR/2820 [Gene]SCO2473/2569 NC_003888 SC7A8.12, probable nitrate reductase, len: 819 aa; similar to SW:NASC_BACSU (EMBL:D50453) Bacillus subtilis assimilatory nitrate reductase catalytic subunit (EC 1.7.99.4) NasC, 710 aa; fasta scores: opt: 1568 z-score: 1572.1 E(): 0; 37.9% identity in 816 aa overlap. Contains Pfam matches to entries PF00384 molybdopterin, Prokaryotic molybdopterin oxidoreductases and PF01568 Molydop_binding, Molydopterin dinucleotide binding domain. Contains also glycine/serine rich region 3 3 8 7 [Reporter]SCO0601 (15C18)_PCR/5200 [Gene]SCO0601/4741 NC_003888 SCF55.25c, putative secreted protein, len: 347 aa; similar to various hypothetical proteins, e.g. TR:CAB45615 (EMBL:AL079356) Streptomyces coelicolor hypothetical 38.9 KD protein SC6G9.24c, 365 aa; fasta scores: opt: 621 z-score: 691.9 E(): 3.7e-31; 35.8% identity in 349 aa overlap. Contains possible N-terminal region signal peptide sequence 2 2 14 14 [Reporter]SCO7742 (9F7)_PCR/2819 [Gene]SCO7742/2568 NC_003888 SC8D11.33, probable MarR-family transcriptional regulator, len: 132 aa; similar to TR:Q9X5U1 (EMBL:AF127374) Streptomyces lavendulae repressor MmcW, 163 aa; fasta scores: opt: 268 z-score: 334.9 E(): 4.1e-11; 36.9% identity in 141 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family. Also contains possible helix-turn-helix motif at residues 63..84 (+3.77 SD) 3 3 8 6 [Reporter]SCO2963 (16O14)_PCR/5199 [Gene]SCO2963/4740 NC_003888 SCE59.22c, putative membrane protein, len: 494 aa. Contains C-terminal region rich in proline and glycine. Contains possible hydrophobic membrane spanning region 2 1 20 3 [Reporter]SCP1.144 (20B4)_PCR/924 [Gene]SCP1.144/849 NC_003888 SCP1.144, possible membrane protein, len: 95aa; contains two possible membrane-spanning hydrophobic regions. 2 2 14 13 [Reporter]SCO6214 (10B7)_PCR/2818 [Gene]SCO6214/2567 NC_003888 SC9G1.04, probable permease, len: 462 aa; similar to SW:PBUX_BACSU (EMBL:X83878) Bacillus subtilis xanthine permease PubX, 438 aa; fasta scores: opt: 1088 z-score: 1244.0 E(): 0; 41.5% identity in 417 aa overlap and to Streptomyces coelicolor St9G1.02, 471 aa; fasta scores: opt: 1668 z-score: 1651.2 E(): 0; 57.0% identity in 435 aa overlap. Contains match to Pfam entry Pfam match to entry PF00860 xan_ur_permease, Xanthine/uracil permeases family and match to Prosite entry PS01116 Xanthine/uracil permeases family signature 2 1 20 2 [Reporter]SCO7498 (20J24)_PCR/923 [Gene]SCO7498/848 NC_003888 SCBAC17A6.31c, possible transcriptional regulator, len: 216 aa; similar to many from Streptomyces coelicolor eg. TR:O50514 (EMBL:AL009204) transcriptional regulator (231 aa) opt: 338, Z-score: 380.0, 37.321% identity (38.614% ungapped) in 209 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and Prosite match to PS00043 Bacterial regulatory proteins, gntR family signature. 2 2 14 12 [Reporter]SCO2367 (11N3)_PCR/2817 [Gene]SCO2367/2566 NC_003888 SCC8A.25c, conserved hypothetical protein, len: 191aa; similar to proteins associated with resistance to tellurium salts e.g. SW:Q52357 (TERD_SERMA) tellurium resistance protein TerD from Serratia marcescens (192 aa) fasta scores; opt: 853, z-score: 1006.2, E(): 0, 67.2% identity in 192 aa overlap. Also strongly similar to others from Streptomyces coelicolor e.g. TR:CAB62770 (EMBL:AL356892) and neighbouring CDS SCC8A.26c. 2 1 19 22 [Reporter]SCO1355 (1J20)_PCR/921 [Gene]SCO1355/847 NC_003888 2SCG61.37c, possible secreted serine protease, len: 537 aa; similar to many eg. SW:P08594 (AQL1_THEAQ) aqualysin I precursor (EC 3.4.21.-) from Thermus aquaticus (513 aa) fasta scores; opt: 1151, z-score: 1199.3, E(): 0, 45.6% identity in 428 aa overlap. Contains Pfam match to entry PF00082 Peptidase_S8, Subtilase family and Prosite matches to PS00136, PS00137 and PS00138 Serine proteases, subtilase family, serine active sites. Also contains possible cleavable N-terminal signal sequence. 2 2 14 11 [Reporter]SCO4125 (12J3)_PCR/2816 [Gene]SCO4125/2565 NC_003888 SCD72A.11, possible acetyltransferase, len: 177 aa; weakly similar to many eg. TR:AAD52985 (EMBL:AF140221) aminoglycoside 6'-N-acetyltransferase from Xanthomonas maltophilia (153 aa) fasta scores; opt: 128, z-score: 164.9, E(): 0.1, 28.7% identity in 115 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 2 1 19 21 [Reporter]SCO6508 (2F20)_PCR/920 [Gene]SCO6508/846 NC_003888 SC1E6.17, gvpK, probable gas vesicle synthesis protein, len: 98 aa; simlar to e.g. Bacillus megaterium TR:O68670 gas vesicle protein GVPK (94 aa), fasta scores; opt: 321 z-score: 536.7 E(): 1.3e-22, 50.5% identity in 93 aa overlap 2 2 14 9 [Reporter]SCO1585 (14B3)_PCR/2814 [Gene]SCO1585/2564 NC_003888 SCI35.07c, hypothetical protein, len: 206 aa; similar to TR:O87860 (EMBL:Z15137) Streptomyces chrysomallus hypothetical protein, 144 aa; fasta scores: opt: 536 Z-score: 638.4 E(): 6.3e-28; 66.929% identity in 127 aa overlap 2 1 19 20 [Reporter]SCO7665 (3B20)_PCR/919 [Gene]SCO7665/845 NC_003888 SC10F4.38c, hypothetical protein, len: 69 aa; similar to TR:Q9XA80 (EMBL:AL096837) Streptomyces coelicolor hypothetical 9.9 kDa protein SCF43A.35c, 88 aa; fasta scores: opt: 205 z-score: 310.7 E(): 9.1e-10; 52.5% identity in 61 aa overlap 2 2 14 8 [Reporter]SCO0088 (14N23)_PCR/2813 [Gene]SCO0088/2563 NC_003888 SCJ11.17c, possible secreted beta-lactamase, len: 319 aa; some similarity to many e.g. SW:BLAB_STRCI partially defined beta-lactamase regulatory protein from Streptomyces cacaoi (312 aa) fasta scores; opt: 196, z-score: 231.8, E(): 1.5e-05, (28.4% identity in 278 aa overlap) and SW:BLAC_BACLI beta-lactamase from Bacillus licheniformis (307 aa) fasta scores; opt: 123, z-score: 148.2, E(): 0.68, (24.3% identity in 267 aa overlap). Contains possible N-terminal signal sequence. 2 1 19 19 [Reporter]SCO7227 (4N16)_PCR/918 [Gene]SCO7227/844 NC_003888 SC2H12.26, possible secreted protein, len: 343 aa. Highly similar to many proteins of undefined function e.g. Escherichia coli SW:YBBK_ECOLI(EMBL:AE000155) (305 aa), fasta scores opt: 705 z-score: 738.7 E():0 42.2% identity in 301 aa overlap and Streptomyces coelicolor TR:Q9X9Z6(EMBL:AL079332) SCI5.04C (319 aa), fasta scores opt: 1209 z-score: 1258.9 E():0 61.9% identity in 281 aa overlap. Contains a possible N-terminal signal sequence. Also contains a Prosite hit to PS01270 Band 7 protein family signature and a Pfam match to entry PF01145 Band_7, SPFH domain / Band 7 family 2 2 14 7 [Reporter]SCO3126 (15J23)_PCR/2812 [Gene]SCO3126/2562 NC_003888 SCE66.05, possible membrane protein, len: 172aa; contains possible membrane-spanning hydrophobic regions. 2 1 19 18 [Reporter]SCO7689 (5J16)_PCR/917 [Gene]SCO7689/843 NC_003888 SC4C2.24, probable ABC transporter ATP-binding protein, len: 551 aa; similar to C-terminal region of SW:YD48_MYCTU (EMBL:Z75555) Mycobacterium tuberculosis hypothetical ABC transporter ATP-binding protein RV1348, 859 aa; fasta scores: opt: 1313 z-score: 1377.6 E(): 0; 43.8% identity in 548 aa overlap. Contains Pfam matches to entries PF00664 ABC_membrane, ABC transporter transmembrane region and PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also contains possible hydrophobic membrane spanning regions 2 2 14 6 [Reporter]SCO5020 (16F23)_PCR/2811 [Gene]SCO5020/2561 NC_003888 SCK15.22, hypothetical protein, len: 178 aa; similar to C-terminal region of TR:Q9KY81 (EMBL:AL356813) Streptomyces coelicolor putative lipoprotein SCK15.23, 221 aa; fasta scores: opt: 444 Z-score: 519.8 E(): 2.5e-21; 39.080% identity in 174 aa overlap 2 1 19 17 [Reporter]SCO7784 (6F16)_PCR/916 [Gene]SCO7784/842 NC_003888 SC5E9.32, possible oxidoreductase, len: 258aa; similar to many eg. SW:P39605 (NFRA_BACSU) nitro/flavin reductase from Bacillus subtilis (249 aa) fasta scores; opt: 426, z-score: 490.0, E(): 8e-20, 32.7% identity in 257 aa overlap. Contains Pfam match to entry PF00881 Nitroreductase, Nitroreductase family. 2 2 14 5 [Reporter]SCO1913 (17B23)_PCR/2810 [Gene]SCO1913/2560 NC_003888 SCI7.31c, possible small membrane protein, len: 88 aa; contains possible hydrophobic membrane spanning region 2 1 19 16 [Reporter]SCO6176 (7B16)_PCR/915 [Gene]SCO6176/841 NC_003888 SC6C5.12c, hypothetical protein, len: 327 aa; similar to TR:Q9S1Z5 (EMBL:AL109747) Streptomyces coelicolor putative secreted protein SCJ21.12c, 279 aa; fasta scores: opt: 474 Z-score: 525.2 E(): 1.3e-21; 41.007% identity in 278 aa overlap 2 1 19 15 [Reporter]SCO5759 (8N12)_PCR/914 [Gene]SCO5759/840 NC_003888 SC7C7.14, unknown, len: 161 aa 4 4 2 15 [Reporter]SCO2122 (7L17)_PCR/7595 [Gene]SCO2122/6919 NC_003888 SC6E10.16c, conserved hypothetical protein, len: 262 aa; unknown function, similar to part of TR:O33970 (EMBL:U80063) Streptomyces cinnamoneus hypothetical protein (199 aa), fasta scores; opt: 827 z-score: 903.5 E(): 0, 61.9% identity in 202 aa overlap and to hypothetical proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. TR:O69572 (EMBL:AL022602) Mycobacterium leprae hypothetical protein (244 aa) (54.0% identity in 215 aa overlap). Similar to others from S.coelicolor e.g. TR:Q9ZBS1 (EMBL:AL034447) S.coelicolor possible acyltransferase (264 aa) (33.2% identity in 250 aa overlap). C-terminus contains 5x slightly degenerate KAAK(A/N)(D/E) repeats. Contains probable coiled-coil from 214 to 255 (42 residues) (Max score: 1.475, probability 0.92) 4 4 2 14 [Reporter]SCO6859 (8H17)_PCR/7594 [Gene]SCO6859/6918 NC_003888 SC7F9.11c, unknown, len: 276 aa. 4 4 2 13 [Reporter]SCO5741 (9D17)_PCR/7593 [Gene]SCO5741/6917 NC_003888 SC9A10.05c, probable membrane protein, len: 184 aa 4 4 2 12 [Reporter]SCO2324 (10P13)_PCR/7592 [Gene]SCO2324/6916 NC_003888 SCC53.15, possible ABC transporter ATP-binding subunit, len: 563aa; similar to many eg. TR:Q9V1Q4 (EMBL:AJ248284) ABC transporter, ATP-binding protein from Pyrococcus abyssi (280 aa) fasta scores; opt: 544, z-score: 493.5, E(): 5.1e-20, 40.4% identity in 230 aa overlap. Contains two Pfam matches to entry PF00005 ABC_tran, ABC transporter, two Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop) and Prosite match to PS00211 ABC transporters family signature. 4 4 2 10 [Reporter]SCO4238 (12H13)_PCR/7590 [Gene]SCO4238/6915 NC_003888 SCD8A.11, probable guanyltransferase, len: 245 aa; similar to TR:O93827 (EMBL:AF030299) Candida albicans mannose-1-phosphate guanyltransferase (EC 2.7.7.13) Psa1, 362 aa; fasta scores: opt: 396 z-score: 478.0 E(): 3.4e-19; 32.4% identity in 244 aa overlap and to SW:MPG1_YEAST (EMBL:U19608) Saccharomyces cerevisiae mannose-1-phosphate guanyltransferase (EC 2.7.7.13) Psa1, 361 aa; fasta scores: opt: 391 z-score: 472.0 E(): 7.3e-19; 31.6% identity in 244 aa overlap. Contains Pfam match to entry PF00483 NTP_transferase, Nucleotidyl transferase 4 4 2 9 [Reporter]SCO0534 (13D13)_PCR/7589 [Gene]SCO0534/6914 NC_003888 SCF11.14, possible beta-hexosaminidase (putative secreted protein), len: 539 aa; similar to TR:Q9ZH39 (EMBL:AF072374) Pseudoalteromonas sp. S9 beta-N-acetylglucosaminidase, 783 aa; fasta scores: opt: 1097 z-score: 1169.9 E(): 0; 36.6% identity in 527 aa overlap and SW:HEX1_VIBFU (EMBL:U41417) Vibrio furnissii beta-hexosaminidase (EC 3.2.1.52) (N-acetyl-beta-glucosaminidase) (beta-N-acetylhexosaminidase) ExoI: fasta scores: opt: 783 z-score: 836.7 E(): 0; 34.1% identity in 478 aa overlap. Contains Pfam match to entry PF00728 glyco_hydro_20, glycosyl hydrolase family 20 4 4 2 8 [Reporter]SCO3496 (14P9)_PCR/7588 [Gene]SCO3496/6913 NC_003888 SCE65.32c, possible lyase precursor, len: 493 aa; low similarity to SW:ALYP_PSESO (EMBL:D10336) Pseudomonas sp. (strain OS-ALG-9) alginate lyase precursor (EC 4.2.2.3) (poly(beta-D-mannuronate) lyase) (poly(ManA) alginate lyase) Aly or AlyP, 398 aa; fasta scores: opt: 550 z-score: 593.8 E(): 1.1e-25; 29.5% identity in 380 aa overlap. Contains possible signal peptide sequence and also a Leucine TTA codon, possible target for bldA regulation 4 4 2 7 [Reporter]SCO1885 (15L9)_PCR/7587 [Gene]SCO1885/6912 NC_003888 SCI7.03c, possible oxidoreductase, len: 385 aa; similar to many proteins of undefined function eg. TR:Q45376 (EMBL:X90711) from a locus required for lipopolysaccharide biosynthesis in Bordetella pertussis (350 aa) fasta scores; opt: 232, z-score: 266.7, E(): 1.6e-07, (28.0% identity in 275 aa overlap). Contains Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family. 3 3 8 5 [Reporter]SCO1790 (17K14)_PCR/5198 [Gene]SCO1790/4739 NC_003888 SCI51.30c, conserved hypothetical protein, len: 210 aa; unknown function, similar to part of TR:O33193 (EMBL:Z98268) Mycobacterium tuberculosis hypothetical protein (250 aa), fasta scores; opt: 560 z-score: 665.7 E(): 1e-29, 49.7% identity in 185 aa overlap. The start codon is uncertain 4 4 2 6 [Reporter]SCO0226 (16H9)_PCR/7586 [Gene]SCO0226/6911 NC_003888 SCJ9A.05c, possible membrane protein, len: 234aa; contains possible peripheral membrane hydrophobic region. 3 3 8 4 [Reporter]SCO1555 (18G14)_PCR/5197 [Gene]SCO1555/4738 NC_003888 SCL11.11c, probable methyltransferase, len: 408 aa; similar to TR:Q55879 (EMBL:D64004) Synechocystis sp. precorrin-6Y methylase CbiE, 425 aa; fasta scores: opt: 379 z-score: 405.5 E(): 3.6e-15; 29.9% identity in 354 aa overlap and to SW:CBIE_SALTY (EMBL:L12006) Salmonella typhimurium precorrin-6Y C5,15-methyltransferase [decarboxylating] (EC 2.1.1.132) CbiE, 201 aa; fasta scores: opt: 250 z-score: 274.2 E(): 7.5e-08; 33.3% identity in 204 aa overlap. Contains Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases 4 4 2 5 [Reporter]SCO0884 (17D9)_PCR/7585 [Gene]SCO0884/6910 NC_003888 SCM1.17c, probable oxidoreductase, len: 477 aa. Highly similar to many mercuric reductases (EC 1.16.1.1) and to dihydrolipoamide dehydrogenases (EC 1.8.1.4)(both enzyme classes belong to the pyridine nucleotide-disulfide oxidoreductases class-I family): Mycobacterium tuberculosis TR:O07268(EMBL:AL021959) mercuric reductase (499 aa), fasta scores opt: 2030 z-score: 2133.1 E():0 63.2% identity in 476 aa overlap and Synechocystis sp. (strain PCC 6803) SW:DLDH_SYNY3(EMBL:D90900) dihydrolipoamide dehydrogenase (EC 1.8.1.4) (473 aa), fasta scores opt: 586 z-score: 619.8 E(): 3.5e-27 29.0% identity in 497 aa overlap. Also highly similar to Streptomyces coelicolor TR:Q9X8C3(EMBL:AL049763) putative pyridine nucleotide-disulfide oxidoreductase SCE36.10C (454 aa), fasta scores opt: 774 z-score: 650.9 E(): 7e-31 40.3% identity in 472 aa overlap. Note that the length of this CDS is more consistent with the dihydrolipoamide dehydrogenases than the mercuric reductases. Contains a Pfam match to entry PF00070 pyr_redox, Pyridine nucleotide-disulphide oxidoreductase class-I. 3 3 8 3 [Reporter]SCO1380 (19C14)_PCR/5196 [Gene]SCO1380/4737 NC_003888 SC10A9.22c, probable DNA damage inducible protein, len: 468 aa; N-terminal region similar to SW:DINP_ECOLI (EMBL:D83536) Escherichia coli DNA-damage-inducible protein P DinP, 351 aa; fasta scores: opt: 574 z-score: 617.7 E(): 5.8e-27; 35.1% identity in 345 aa overlap and C-terminal region similar to N-terminal region of TR:Q9X7X7 (EMBL:AL049485) Streptomyces coelicolor probable zinc-binding alcohol dehydrogenase SC6A5.31c, 363 aa; blastp scores: Score = 82 (28.9 bits), Expect = 3.4, P = 0.97; Identities = 37/112 (33%), Positives = 50/112 (44%). Contains Pfam match to entry PF00817 IMS, impB/mucB/samB family 3 3 8 2 [Reporter]SCP1.178 (20K10)_PCR/5195 [Gene]SCP1.178/4736 NC_003888 SCP1.178, unknown, len: 78aa; 3 3 7 22 [Reporter]SCO4215 (1K6)_PCR/5193 [Gene]SCO4215/4735 NC_003888 2SCD46.29, possible GntR-family regulatory protein, len: 252 aa; similar to many regulators of possible integrative plasmid origin e.g. TR:Q07191 (EMBL:Z19589) from plasmid pSAM2 of Streptomyces ambofaciens (259 aa) fasta scores; opt: 475, z-score: 554.1, E(): 2.1e-23, 34.6% identity in 246 aa overlap and TR:Q9X8D2 (EMBL:AL049573) KorSA, regulatory protein from Streptomyces coelicolor (259 aa) fasta scores; opt: 485, z-score: 565.6, E(): 4.9e-24, 35.0% identity in 257 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. 3 3 7 21 [Reporter]SCO6992 (2G6)_PCR/5192 [Gene]SCO6992/4734 NC_003888 SC8F11.18c, absR1, regulatory protein, len: 359 aa. Identical to that previously sequenced: Streptomyces coelicolor TR:AAF19103(EMBL:AF136167) putative activator of secondary metabolism, AbsR1. 3 3 7 18 [Reporter]SCO6845 (5K2)_PCR/5189 [Gene]SCO6845/4733 NC_003888 SC3D9.13c, unknown, len: 206 aa. Similar in parts to Agrobacterium tumefaciens (Ti plasmid pTiC58) TR:BAA87688 (EMBL:AB016260) hypothetical protein, TiOrf63 (312 aa), fasta scores opt: 466 z-score: 575.5 E(): 1.2e-24 44.3% identity in 167 aa overlap. 3 3 7 17 [Reporter]SCO6630 (6G2)_PCR/5188 [Gene]SCO6630/4732 NC_003888 SC4G2.04, unknown, len: 67 aa; similar to S. coelicolor TR:Q53896 (EMBL:X60316) AbaA gene ORFD (75 aa), fasta scores; opt: 120 z-score: 206.5 E(): 0.00029, 43.8% identity in 48 aa overlap 2 2 14 4 [Reporter]SCO4985 (18N19)_PCR/2809 [Gene]SCO4985/2559 NC_003888 2SCK36.08, unknown, len: 284 aa 3 3 7 16 [Reporter]SCO0303 (7C2)_PCR/5187 [Gene]SCO0303/4731 NC_003888 SC5G9.12c, putative secreted protein, len: 68 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 2 2 14 3 [Reporter]SCO6311 (19J19)_PCR/2808 [Gene]SCO6311/2558 NC_003888 SCIF3.13, possible integral membrane protein, len: 140 aa. Contains possible hydrophobic membrane spanning regions 3 3 7 15 [Reporter]SCO6966 (7O22)_PCR/5186 [Gene]SCO6966/4730 NC_003888 SC6F7.19c, possible lipase, len: 269 aa. Similar to many other putative lipases e.g. Thermotoga maritima TR:Q9X171(EMBL:AE001789) lipase, putative (259 aa), fasta scores opt: 345 z-score: 396.1 E(): 1.3e-14 33.1% identity in 239 aa overlap. contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 2 1 19 14 [Reporter]SCO5403 (9J12)_PCR/913 [Gene]SCO5403/839 NC_003888 SC8F4.07, probable two-component system response regulator, len: 222 aa; similar to many e.g. SW:Q02540 (COPR_PSESM) CopR transcriptional activator protein from Pseudomonas syringae (pv. tomato) (227 aa) fasta scores; opt: 621, z-score: 735.4, E(): 0, 45.7% identity in 223 aa overlap. Contains a Pfam match to entry PF00072 response_reg, Response regulator receiver domain and Pfam match to entry PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal. 2 2 14 2 [Reporter]SCO3976 (20B19)_PCR/2807 [Gene]SCO3976/2557 NC_003888 SCBAC25E3.13c, possible phosphodiesterase, len: 275aa: similar to many eg. TR:Q9CDC5 (EMBL:AL583917) putative glycerophosphoryl diester phosphodiesterase from Mycobacterium leprae (271 aa) fasta scores; opt: 416, Z-score: 496.0, 46.269% identity (49.012% ungapped) in 268 aa overlap and SW:P37965 (GLPQ_BACSU) glycerophosphoryl diester phosphodiesterase from Bacillus subtilis (293 aa) fasta scores; opt: 233, Z-score: 281.0, 33.712% identity (36.777% ungapped) in 264 aa overlap. 2 1 19 13 [Reporter]SCO6244 (10F12)_PCR/912 [Gene]SCO6244/838 NC_003888 SCAH10.09c, hypothetical protein, len: 207 aa; highly similar to TR:P72363 (EMBL:U63518) Streptomyces arenae ORFuMS, len: 201 aa; fasta scores: opt: 498 z-score: 585.3 E(): 3.2e-25; 48.8% identity in 209 aa overlap 2 2 13 22 [Reporter]SCO0994 (1B15)_PCR/2805 [Gene]SCO0994/2556 NC_003888 2SCG2.07, possible integral membrane protein, len: 421 aa; similar to TR:O86692 (EMBL:AL031371) Streptomyces coelicolor putative transport system permease protein SC4G2.20, 436 aa; fasta scores: opt: 354 z-score: 396.0 E(): 1.6e-14; 29.6% identity in 429 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 19 12 [Reporter]SCO2603 (11B12)_PCR/911 [Gene]SCO2603/837 NC_003888 SCC88.14, possible integrase, len: 518 aa; similar to TR:Q38184 (EMBL:X85213) Bacteriophage TP901-1 integrase, 485 aa; fasta scores: opt: 212 z-score: 243.5 E(): 3.8e-06; 24.2% identity in 417 aa overlap. Contains TTA leucine codon, possible target for bldA regulation 2 2 13 21 [Reporter]SCO2387 (2N11)_PCR/2804 [Gene]SCO2387/2555 NC_003888 SC4A7.15, fabD, malonyl CoA:acyl carrier protein malonyltransferase, len: 316 aa; identical to previously sequenced TR:P72391 (EMBL:X86475) Streptomyces coelicolor malonyl CoA:acyl carrier protein malonyltransferase, FabD, 316 aa and similar to SW:FABD_ECOLI (EMBL:M87040) Escherichia coli malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39) FabD, 308 aa; fasta scores: opt: 524 z-score: 551.4 E(): 2.5e-23; 36.8% identity in 315 aa overlap. Contains Pfam match to entry PF00698 Acyl_transf, Acyl transferase domain 2 1 19 11 [Reporter]SCO4639 (12N8)_PCR/910 [Gene]SCO4639/836 NC_003888 SCD82.10, hypothetical protein, len: 201 aa; similar to TR:CAB52009 (EMBL:AL109663) Streptomyces coelicolor putative transcriptional regulator SC4A10.33, 209 aa; fasta scores: opt: 138 z-score: 174.2 E(): 0.028; 30.3% identity in 165 aa overlap 2 2 13 20 [Reporter]SCO5913 (3J11)_PCR/2803 [Gene]SCO5913/2554 NC_003888 SC10A5.18, probable secreted protease, len: 411 aa; similar to many members of the peptidase family S2A eg. PRT C_STRGR P52320 serine protease c precursor (ec 3.4.21.-) (4 57 aa), fasta scores; opt: 326 z-score: 460.9 E(): 1.8e-18, 29.8% identity in 420 aa overlap. Contains N-terminal sign al sequence and PS00134 Serine proteases, trypsin family, h istidine active site. Also contains TTA leucine codon, a po tential target for action of bldA 2 1 19 10 [Reporter]SCO3094 (13J8)_PCR/909 [Gene]SCO3094/835 NC_003888 SCE41.03c, conserved hypothetical protein, len: 186 aa; similar to TR:P96375 (EMBL:Z92539) Mycobacterium tuberculosis hypothetical 16.6 kDa protein MTCY10G2.24c, 155 aa; fasta scores: opt: 632 z-score: 730.7 E(): 0; 67.6% identity in 136 aa overlap 2 2 13 19 [Reporter]SCO6020 (4F11)_PCR/2802 [Gene]SCO6020/2553 NC_003888 SC1C3.08c, possible transcriptional regulator, len: 366 aa; some similarity to many putative transcriptional regulators, e.g. TR:P96499 (EMBL:U56901) B. subtilis putative transcriptional regulator YVHJ (391 aa), fasta scores; opt:283 z-score: 276.9 E(): 3.4e-08, 28.2% identity in 365 aaoverlap. Contains Highly basic N-terminus, followed by hydrophobic region (possibly transmembrane) 2 1 19 9 [Reporter]SCO0857 (14F8)_PCR/908 [Gene]SCO0857/834 NC_003888 SCM2.10, possible TetR-family regulator, len: 242 aa. Similar to Streptomyces coelicolor TR:Q9X8G9(EMBL:AL049819) putative transcriptional regulator SCE7.13c (204 aa), fasta scores opt: 455 z-score: 536.7 E(): 1.6e-22 41.1% identity in 202 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and an N-terminal 3x AAG repeat, between residues 5..15. 2 2 13 18 [Reporter]SCO5450 (5B11)_PCR/2801 [Gene]SCO5450/2552 NC_003888 SC3D11.07c, probable ABC transporter, len: 627aa; similar to many eg. TRNEW:CAB66302 (EMBL:AL136519) ABC transporter protein, ATP-binding component from Streptomyces coelicolor (1243 aa) fasta scores; opt: 1238, z-score: 1250.8, E(): 0, 38.7% identity in 633 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, Pfam match to entry PF00664 ABC_membrane, ABC transporter transmembrane region, Prosite match to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 2 1 19 8 [Reporter]SCO0858 (15B8)_PCR/907 [Gene]SCO0858/833 NC_003888 SCM2.11c, unknown, len: 125 aa. 2 2 13 17 [Reporter]SCO5833 (6N7)_PCR/2800 [Gene]SCO5833/2551 NC_003888 SC5B8.23c, unknown, len: 78 aa 2 1 19 7 [Reporter]SCO2892 (16N4)_PCR/906 [Gene]SCO2892/832 NC_003888 SCE6.29, possible secreted protein, len: 454 aa. Similar to another putative secreted protein from Streptomyces coelicolor: TR:CAB56661(EMBL:AL121596) SCF51A.09C (442 aa), fasta scores opt: 614 z-score: 649.3 E(): 9.8e-29 31.3% identity in 454 aa overlap. Contains a possible N-terminal signal sequence. 2 2 13 16 [Reporter]SCO6759 (7J7)_PCR/2799 [Gene]SCO6759/2550 NC_003888 SC6A5.08, probable phytoene synthase, len: 303aa; similar to many eg. TR:Q50892 (EMBL:Z21955) phytoene synthase from the light-induced carotenoid biosynthesis cluster of Myxococcus xanthus (336 aa) fasta scores; opt: 322, z-score: 379.7, E(): 7.5e-14, (31.8% identity in 274 aa overlap). Contains Pfam match to entry PF00494 SQS_PSY, Squalene and phytoene synthases, score 34.70, E-value 1.2e-08. 2 1 19 6 [Reporter]SCO0673 (17J4)_PCR/905 [Gene]SCO0673/831 NC_003888 SCF91.33c, unknown, len: 175 aa. Weakly similar to several putative regulatory proteins including: Streptomyces coelicolor TR:CAB42940(EMBL:AL049863) SC5H1.15C (151 aa), fasta scores opt: 235 z-score: 290.0 E(): 8.9e-09 39.3% identity in 122 aa overlap. 2 1 19 5 [Reporter]SCO0939 (18F4)_PCR/904 [Gene]SCO0939/830 NC_003888 SCM10.27c, probable hydrolase, len: 287 aa; similar to TR:CAB56657 (EMBL:AL121596) Streptomyces coelicolor putative hydrolase SCF51A.05, 278 aa; fasta scores: opt: 325 z-score: 381.1 E(): 7.7e-14; 32.9% identity in 283 aa overlap and to SW:PRXC_STRAU (EMBL:AF031242) Streptomyces aureofaciens non-heme chloroperoxidase (EC 1.11.1.10) CpoT, 278 aa; fasta scores: opt: 239 z-score: 282.3 E(): 2.5e-08; 30.4% identity in 253 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 4 4 2 4 [Reporter]SCO0377 (18P5)_PCR/7584 [Gene]SCO0377/6909 NC_003888 SCF62.03c, possible integral membrane protein, len: 177 aa. Contains multiple possible membrane spanning hydrophobic domains. 4 4 2 3 [Reporter]SCP1.152 (19L5)_PCR/7583 [Gene]SCP1.152/6908 NC_003888 SCP1.152, unknown, len: 150aa; 4 4 2 2 [Reporter]SCP1.217 (20D5)_PCR/7582 [Gene]SCP1.217/6907 NC_003888 SCP1.217, unknown, len: 69aa; 4 4 1 22 [Reporter]SCO5180 (1D1)_PCR/7580 [Gene]SCO5180/6906 NC_003888 2SC3B6.04c, peptidase, len: 516 aa; highly similar to TR:Q54399 (EMBL:L42759) Streptomyces lividans proteinase SlpE, 513 aa; fasta scores: opt: 1543 z-score: 1741.5 E(): 0; 50.2% identity in 526 aa overlap and to Streptomyces coelicolor putative peptidase 2SC3B6.03c, 513 aa; fasta scores: opt: 1544 z-score: 1465.5 E(): 0; 50.2% identity in 526 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 4 4 1 21 [Reporter]SCO1758 (1P21)_PCR/7579 [Gene]SCO1758/6905 NC_003888 2SCI34.11c, probable GTP binding protein, len: 492 aa; similar to SW:ENGA_BACSU (EMBL:L47648) Bacillus subtilis probable GTP binding protein EngA, 436 aa; fasta scores: opt: 1143 z-score: 1175.4 E(): 0; 42.0% identity in 436 aa overlap. Contains Pfam match to entry PF01926 MMR_HSR1, GTPase of unknown function and 2x matches to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 4 1 20 [Reporter]SCO4947 (2L21)_PCR/7578 [Gene]SCO4947/6904 NC_003888 2SCK31.07, narG3, nitrate reductase alpha chain, len: 1227 aa; highly similar to SW:NARG_BACSU (EMBL:Z49884) Bacillus subtilis nitrate reductase alpha chain (EC 1.7.99.4)NarG, 1228 aa; fasta scores: opt: 4080 z-score: 4618.0 E(): 0; 49.5% identity in 1206 aa overlap and to TR:O86717 (EMBL:AL031515) Streptomyces coelicolor NarG, putative nitrate reductase alpha chain SC5C7.20, 1231 aa; fasta scores: opt: 5648 z-score: 5294.1 E(): 0; 66.6% identity in 1232 aa overlap. Contains Pfam matches to entries PF00384 molybdopterin, Prokaryotic molybdopterin oxidoreductases and PF01568 Molydop_binding, Molydopterin dinucleotide binding domain and matches to Prosite entries PS00551 Prokaryotic molybdopterin oxidoreductases signature 1 and PS00490 Prokaryotic molybdopterin oxidoreductases signature 2 4 4 1 19 [Reporter]SCO7386 (3H21)_PCR/7577 [Gene]SCO7386/6903 NC_003888 SC10G8.13c, possible secreted serine protease, len: 390 aa. Similar to many serine proteases e.g. Streptomyces griseus SW:PRTC_STRGR(EMBL:L29018) serine protease C precursor (EC 3.4.21.-) (457 aa), fasta scores opt: 983 z-score: 1020.1 E(): 0 44.4% identity in 403 aa overlap and Streptomyces sp. TR:Q55353(EMBL:X74102) alkaline serine protease II (382 aa), fasta scores opt: 959 z-score: 996.4 E():0 44.7% identity in 403 aa overlap. Contains a Prosite hits to PS00134 Serine proteases, trypsin family, histidine active site PS00135 Serine proteases, trypsin family, serine active site and a Pfam match to entry PF00089 trypsin, Trypsin. Also contains a possible N-terminal signal sequence. 4 4 1 18 [Reporter]SCO5873 (4D21)_PCR/7576 [Gene]SCO5873/6902 NC_003888 SC2E9.14, unknown, len: 154 aa; similar to Mycobacterium tuberculosis hypothetical protein TR:O07196 (EMBL:Z96072) MTCY05A6.15C (122 aa), fasta scores; opt: 299 z-score: 502.1 E(): 9.1e-21, 41.0% identity in 117 aa overlap 3 3 7 14 [Reporter]SCO7194 (8K22)_PCR/5185 [Gene]SCO7194/4729 NC_003888 SC8A11.22, possible polysaccharide biosynthesis protein, len: 271 aa; similar to N-terminal region of SW:SPSK_BACSU (EMBL:X73124) Bacillus subtilis spore coat polysaccharide biosynthesis protein SpsK, 432 aa; fasta scores: opt: 278 z-score: 331.5 E(): 5.3e-11; 28.1% identity in 242 aa overlap 4 4 1 17 [Reporter]SCO6702 (5P17)_PCR/7575 [Gene]SCO6702/6901 NC_003888 SC4C6.12c, pcaJ, probable 3-oxoadipate CoA-transferase subunit B, len: 217 aa; highly similar to many CoA transferases e.g. SW:PCAJ_PSEPU (EMBL:M88763), PcaJ, Pseudomonas putida 3-oxoadipate CoA-transferase subunit B (212 aa), fasta scores; opt: 684 z-score: 774.2 E(): 0, 52.9% identity in 206 aa overlap. Highly similar to SW:SCOB_MYCTU (EMBL:Z95556), ScoB, Mycobacterium tuberculosis probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (218 aa) (73.9% identity in 207 aa overlap). Contains Pfam match to entry PF01144 CoA_trans, Coenzyme A transferase 3 3 7 13 [Reporter]SCO6061 (9G22)_PCR/5184 [Gene]SCO6061/4728 NC_003888 SC9B1.08, possible oxidoreductase, len: 135aa; highly conserved in prokaryotes and eukaryotes eg. TR:P71971 (EMBL:Z80225) from Mycobacterium tuberculosis (136 aa) fasta scores; opt: 653, z-score: 835.9, E(): 0, (71.1% identity in 128 aa overlap) and TR:CAB40164 (EMBL:AL049558) from Schizosaccharomyces pombe (138 aa) fasta scores; opt: 475, z-score: 612.0, E(): 9e-27, (48.1% identity in 135 aa overlap). Also similar to SW:PMSR_STRPN Peptide methionine sulfoxide reductase from Streptococcus pneumoniae (312 aa) fasta scores; opt: 331, z-score: 426.0, E(): 2.1e-16, (46.2% identity in 132 aa overlap). 4 4 1 16 [Reporter]SCO2172 (6L17)_PCR/7574 [Gene]SCO2172/6900 NC_003888 SC5F7.29c, unknown, len: 82aa; 3 3 7 12 [Reporter]SCO2428 (10C22)_PCR/5183 [Gene]SCO2428/4727 NC_003888 SCC42.09, possible phosphate binding protein (putative secreted protein), len: 522 aa; C-terminal domain similar to TR:Q9Z9M3 (EMBL:AB017492) Pseudomonas aeruginosa phosphate binding protein PtpS, 323 aa; fasta scores: opt: 226 z-score: 266.2 E(): 2.3e-07; 25.3% identity in 328 aa overlap. Contains Pfam match to entry PF01449 PstS, Phosphate-binding protein and possible N-terminal region signal peptide sequence 3 3 7 11 [Reporter]SCO4613 (11O18)_PCR/5182 [Gene]SCO4613/4726 NC_003888 SCD39.13, possible integral membrane protein, len: 88 aa; similar to SW:YEAQ_ECOLI (EMBL:AE000274) Escherichia coli hypothetical 8.7 kDa protein in GapA-Rnd intergenic region YeaQ, 82 aa; fasta scores: opt: 233 z-score: 300.6 E(): 2.8e-09; 42.5% identity in 80 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 7 10 [Reporter]SCO2918 (12K18)_PCR/5181 [Gene]SCO2918/4725 NC_003888 SCE19A.18, probable nicotinamidase, len: 195 aa; highly similar to nicotinamidases e.g. TR:Q50575 (EMBL:U59967), PncA, Mycobacterium tuberculosis pyrazinamidase/nicotinamidase (186 aa), fasta scores; opt: 801 z-score: 921.3 E(): 0, 65.6% identity in 186 aa overlap. Contains Pfam match to entry PF00857 Isochorismatase, Isochorismatase family 3 3 7 9 [Reporter]SCO1822 (13G18)_PCR/5180 [Gene]SCO1822/4724 NC_003888 SCI8.07c, possible transmembrane transport protein, len: 544 aa. A member of the sodium:solute symporter family it is similar to many including: Streptomyces coelicolor TR:O70003 (EMBL: AL022374) putative transmembrane transport SC5B8.17 (530 aa), fasta scores opt: 2215 z-score: 2454.2 E(): 0 64.5% identity in 527 aa overlap and Pseudomonas putida TR:O50471 (EMBL: Z71176) phenylacetic acid permease (520 aa), fasta scores opt: 1004 z-score: 1114.3 E():0 46.3% identity in 527 aa overlap. Contains a Pfam match to entry PF00474 SSF, Sodium:solute symporter family and multiple possible membrane spanning hydrophobic domains. 3 3 7 8 [Reporter]SCO1562 (14C18)_PCR/5179 [Gene]SCO1562/4723 NC_003888 SCL11.18, possible integral membrane protein, len: 138 aa. Contains possible hydrophobic membrane spanning regions 3 3 7 7 [Reporter]SCO0690 (15O14)_PCR/5178 [Gene]SCO0690/4722 NC_003888 SCF15.11, possible oxidoreductase, molybdopterin binding subunit, len: 330 aa. Similar to many molybdopterin binding subunits of multipartite proteins including: Thauera aromatica TR:O33820 (EMBL:AJ001830) 4-hydroxybenzoyl-coA reductase Beta-subunit (324 aa), fasta scores opt: 425 z-score: 493.1 E(): 4.3e-20 36.1% identity in 316 aa overlap and Pseudomonas putida TR:P72222 (EMBL:X98131) quinoline 2-oxidoreductase, QorM (288 aa), fasta scores opt: 160 z-score: 190.3 E(): 0.0032 25.5% identity in 325 aa overlap. Contains a Pfam match to entry PF00941 dehydrog_molyb, Molybdopterin binding domain in dehydrogenase. 2 2 13 15 [Reporter]SCO4765 (8F7)_PCR/2798 [Gene]SCO4765/2549 NC_003888 SC6G4.43c, unknown, len: 233 aa; similar to hypothet ical proteins e.g. M. tuberculosis TR:P95151 (EMBL:Z83859) MTCY359.09 (251 aa), fasta scores; opt: 716 z-score: 609.7 E(): 1e-26, 54.4% identity in 215 aa overlap 3 3 7 6 [Reporter]SCO3548 (16K14)_PCR/5177 [Gene]SCO3548/4721 NC_003888 SCH5.11c, probable anti-sigma factor, len: 143 aa; similar to many e.g. SW:RSBW_BACSU RsbW, anti-sigma B factor from Bacillus subtilis (160 aa) fasta scores; opt: 153, z-score: 198.9, E(): 0.00089, (28.7% identity in 136 aa overlap) and SW:SP22_BACSU SpoIIAB, anti-sigma F factor, sporulation protein, from Bacillus subtilis (146 aa) fasta scores; opt: 125, z-score: 165.8, E(): 0.063, (29.2% identity in 137 aa overlap). 2 2 13 14 [Reporter]SCO7737 (9B7)_PCR/2797 [Gene]SCO7737/2548 NC_003888 SC8D11.28, unknown, len: 170 aa 3 3 7 5 [Reporter]SCO1542 (17G14)_PCR/5176 [Gene]SCO1542/4720 NC_003888 SCL2.32, hypothetical protein, len: 186 aa; similar to various hypothetical proteins, e.g. TR:CAB59700 (EMBL:AL132707) Streptomyces coelicolor hypothetical 21.7 kD protein SCF51.02c, 205 aa; fasta scores: opt: 188 z-score: 240.3 E(): 5.4e-06; 31.1% identity in 180 aa overlap 2 1 19 4 [Reporter]SCP1.293c (19B4)_PCR/903 [Gene]SCP1.293c/829 NC_003888 SCP1.293c, possible conserved DNA-binding protein, len: 290aa; similar to many from streptomycetes eg. TR:Q9RHR7 (EMBL:AB025200) ORF312 protein from plasmid pNO33 of Streptomyces albulus (285 aa) fasta scores; opt: 857, z-score: 1027.5, E(): 0, and TR:CAB88448 (EMBL:AL353815) putative DNA-binding protein from Streptomyces coelicolor (295 aa) fasta scores; opt: 706, z-score: 847.4, E(): 0, 40.8% identity in 282 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix. 2 2 13 13 [Reporter]SCO6270 (10N3)_PCR/2796 [Gene]SCO6270/2547 NC_003888 SCAH10.35c, possible oxidoreductase alpha-subunit, len: 630 aa; similar to TR:O53182 (EMBL:AL021246) Mycobacterium tuberculosis oxidoreductase alpha-subunit, 653 aa; fasta scores: opt: 2008 z-score: 2153.9 E(): 0; 66.4% identity in 614 aa overlap 2 1 19 3 [Reporter]SCP1.210c (19N24)_PCR/902 [Gene]SCP1.210c/828 NC_003888 SCP1.210c, possible membrane protein, len: 109aa; contains two possible membrane-spanning hydrophobic regions. 2 2 13 12 [Reporter]SCO2362 (11J3)_PCR/2795 [Gene]SCO2362/2546 NC_003888 SCC8A.20c, unknown, len: 839 aa; regions similar to others of undefined function e.g. TR:AAF11767 (EMBL:AE002054) hypothetical protein from Deinococcus radiodurans (353 aa) fasta scores; opt: 463, z-score: 489.2, E(): 8.7e-20, 40.3% identity in 355 aa overlap 2 1 19 2 [Reporter]SCO5061 (20F24)_PCR/901 [Gene]SCO5061/827 NC_003888 SCBAC20F6.04, possible ATP/GTP binding protein, len: 362 aa; highly similar to SW:YCHF_ECOLI (EMBL:AE000219) Escherichia coli probable GTP-binding protein in treA-pth intergenic region YchF or Gtp1 or B1203, 362 aa; fasta scores: opt: 1193 Z-score: 1225.2 bits: 235.3 E(): 1.2e-60; 51.944% identity in 360 aa overlap. Contains Pfam match to entry PF01018 GTP1_OBG, GTP1/OBG family and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 2 13 11 [Reporter]SCO4780 (12F3)_PCR/2794 [Gene]SCO4780/2545 NC_003888 SCD63.12, probable aldehyde dehydrogenase, len: 537 aa; similar to SW:DHAB_BACSU (EMBL:U47861) Bacillus subtilis betaine aldehyde dehydrogenase (EC 1.2.1.8) GbsA, 490 aa; fasta scores: opt: 950 z-score: 1089.2 E(): 0; 37.4% identity in 460 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family and match to Prosite entry PS00687 Aldehyde dehydrogenases glutamic acid active site 2 1 18 22 [Reporter]SCO1132 (1F20)_PCR/899 [Gene]SCO1132/826 NC_003888 2SCG38.25c, possible oxidoreductase, len: 715 aa; similar to TR:AAG05321 (EMBL:AE004619) Pseudomonas aeruginosa probable hydroxylase large subunit PA1933, 734 aa; fasta scores: opt: 1323 z-score: 1465.6 E(): 0; 44.2% identity in 737 aa overlap and to TR:CAB69663 (EMBL:AL137165) Streptomyces coelicolor putative oxidoreductase SCF42.01, 369 aa; fasta scores: opt: 1286 z-score: 1141.0 E(): 0; 55.4% identity in 359 aa overlap. Contains 3x Pfam matches to entry PF01315 Ald_Xan_dh_C, Aldehyde oxidase and xanthine dehydrogenase, C terminus 2 2 13 10 [Reporter]SCO3188 (13B3)_PCR/2793 [Gene]SCO3188/2544 NC_003888 SCE22.05, possible methylthioadenosine phosphorylase, len: 280 aa; similar to TR:CAB49985 (EMBL:AJ248286) Pyrococcus abyssi methylthioadenosine phosphorylase related (MtaP), 265 aa; fasta scores: opt: 711 z-score: 861.9 E(): 0; 43.4% identity in 265 aa overlap and to SW:MTAP_HUMAN (EMBL:U22233) Homo sapiens 5'-methylthioadenosine phosphorylase (EC 2.4.2.28) (MTAPase) MtaP, 283 aa; fasta scores: opt: 653 z-score: 791.7 E(): 0; 38.2% identity in 254 aa overlap. Contains Pfam match to entry PF00896 Mtap_PNP, phosphorylases family 2 2 1 18 21 [Reporter]SCO6502 (2B20)_PCR/898 [Gene]SCO6502/825 NC_003888 SC1E6.11, gvpG, probable gas vesicle synthesis protein, len: 87 aa; simlar to e.g. TR:O68673 (EMBL:AF053765) gas vesicle protein GVPG (88 aa), fasta scores; opt: 152 z-score: 269.8 E(): 9.5e-08, 49.1% identity in 53 aa overlap. Contains probable coiled-coil from aa 37 to 76 2 2 13 9 [Reporter]SCO1765 (13N23)_PCR/2792 [Gene]SCO1765/2543 NC_003888 SCI51.05c, hypothetical protein, len: 250 aa; unknown function, similar to SW:Y002_METJA (EMBL:U67459) Methanococcus jannaschii hypothetical protein (243 aa), fasta scores; opt: 290 z-score: 356.7 E(): 1.6e-12, 28.3% identity in 223 aa overlap. There is some overlap with the C-terminus of SCI51.04 and weak similarity with SCI51.04 (253 aa) (29.6% identity in 233 aa overlap) 2 1 18 20 [Reporter]SCO7695 (3N16)_PCR/897 [Gene]SCO7695/824 NC_003888 SC1A4.03c, hypothetical protein, len: 122 aa; similar to TR:Q9K3Q6 (EMBL:AL360034) Streptomyces coelicolor conserved hypothetical protein 2SCG4.08, 130 aa; fasta scores: opt: 140 z-score: 184.4 E(): 0.0098; 31.3% identity in 112 aa overlap 2 2 13 8 [Reporter]SCO1651 (14J23)_PCR/2791 [Gene]SCO1651/2542 NC_003888 SCI41.34c, hypothetical protein, len: 300 aa. N-terminal identical to previously sequenced TR:O87591 (EMBL:AF086832) Streptomyces coelicolor hypothetical 18.8 kd protein (fragment) (168 aa); fasta scores: opt: 1179 z-score: 1395.1 E(): 0; 100.0% identity in 168 aa overlap and similar to various hypothetical proteins, e.g. TR:Q9ZG14 (EMBL:AF088800) Rhodococcus erythropolis hypothetical 35.2 Kd protein, 324 aa; fasta scores: opt: 1134 z-score: 1338.1 E():0; 59.9% identity in 279 aa overlap 2 1 18 19 [Reporter]SCO7223 (4J16)_PCR/896 [Gene]SCO7223/823 NC_003888 SC2H12.22, possible monooxygenase, len: 373 aa. Similar to many other monooxygenases e.g. Sphingomonas sp. TR:P96555(EMBL:AB000564) salicylate hydroxylase (395 aa), fasta scores opt: 347 z-score: 390.9 E(): 2.7e-14 27.0% identity in 378 aa overlap and Streptomyces coelicolor TR:Q9RK22(EMBL:AL109974) putative monooxygenase, SCF34.03C (388 aa), fasta scores opt: 998 z-score: 1113.7 E():0 47.2% identity in 343 aa overlap. Contains a Pfam match to entry PF01360 Monooxygenase, Monooxygenase. 2 2 13 7 [Reporter]SCO1888 (15F23)_PCR/2790 [Gene]SCO1888/2541 NC_003888 SCI7.06c, probable dihydroxy-acid dehydratase, len: 576aa; similar to many eg. SW:ILVD_BACSU dihydroxy-acid dehydratase from Bacillus subtilis (557 aa) fasta scores; opt: 1153, z-score: 1290.1, E(): 0, (37.0% identity in 560 aa overlap). Contains Pfam match to entry PF00920 ILVD_EDD, Dehydratase family and Prosite match to PS00886 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1. Also contains a helix-turn-helix motif (+3.32 SD) 207-228aa. 2 1 18 18 [Reporter]SCO2400 (5F16)_PCR/895 [Gene]SCO2400/822 NC_003888 SC4A7.28c, putative membrane protein, len: 249 aa. Contains 1 possible hydrophobic membrane spanning region 2 2 13 6 [Reporter]SCO1725 (16B23)_PCR/2789 [Gene]SCO1725/2540 NC_003888 SCI11.14c, possible secreted hydrolase, len: 268 aa; similar to TR:Q59837 (EMBL:X92765), estA, Streptomyces diastatochromogenes esterase A (326 aa), fasta scores; opt: 147 z-score: 171.2 E(): 0.033, 26.8% identity in 314 aa overlap. The difference in length is accounted for by internal deletions in this protein, relative to TR:Q59837. The putative active-site serine motif GDSYT in the S.diastatochromogenes enzyme is conserved in this protein. Contains a probable N-terminal signal sequence 2 1 18 17 [Reporter]SCO7777 (6B16)_PCR/894 [Gene]SCO7777/821 NC_003888 SC5E9.25c, possible hydrolase, len: 309aa; weakly similar to many eg. TR:O69356 (EMBL:D88016) 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Rhodococcus erythropolis (281 aa) fasta scores; opt: 206, z-score: 247.2, E(): 2.7e-06, 26.3% identity in 281 aa overlap. 2 1 18 16 [Reporter]SCO5651 (7N12)_PCR/893 [Gene]SCO5651/820 NC_003888 SC6A9.16, unknown, len: 394 aa 3 3 7 4 [Reporter]SCO3459 (18C14)_PCR/5175 [Gene]SCO3459/4719 NC_003888 SCE46.16c, hypothetical protein, len: 287 aa; similar to various hypothetical proteins, e.g. TR:CAB52055 (EMBL:AL109732) Streptomyces coelicolor hypothetical 24.3kD protein; fasta scores: opt: 165 z-score: 195.9 E(): 0.0014; 37.4% identity in 131 aa overlap 3 3 7 3 [Reporter]SCP1.30 (19O10)_PCR/5174 [Gene]SCP1.30/4718 NC_003888 SCP1.30, unknown, len: 187aa; 3 3 7 2 [Reporter]SCO6000 (20G10)_PCR/5173 [Gene]SCO6000/4717 NC_003888 SCBAC1C11.03, hypothetical protein, len: 81 aa; low similarity to TR:Q9RDI8 (EMBL:AL136519) Streptomyces coelicolor hypothetical 7.0 kDa protein SCC57A.09c, 65 aa; fasta scores: opt: 135 z-score: 199.2 E(): 0.0015; 46.2% identity in 52 aa overlap 3 3 6 22 [Reporter]SCO5391 (1G6)_PCR/5171 [Gene]SCO5391/4716 NC_003888 2SC6G5.35, possible ATP/GTP-binding protein, doubtful CDS, len: 110aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 3 6 21 [Reporter]SCO0790 (2C6)_PCR/5170 [Gene]SCO0790/4715 NC_003888 SCF43.01, possible hydrolase, partial CDS, len: > 98 aa. Identical over the available sequence to Streptomyces lividans TR:P72465(EMBL:Z70724) substrate hyphae associated protein, Orf2 (218 aa), fasta scores opt: 648 z-score: 826.2 E(): 0 100.0% identity in 98 aa overlap. Also similar to several phosphatases e.g. Streptomyces collinus TR:Q9X654(EMBL:AF131877) NapH phosphatase (231 aa), fasta scores opt: 186 z-score: 245.7 E(): 2.6e-06 41.9% identity in 86 aa overlap.,3SCF60.22, possible hydrolase (fragment), len: >154 aa; identical to N-terminal region of TR:P72465 (EMBL:Z70724) Streptomyces lividans coat protein ORF2, 218 aa and similar to TR:Q9RWE0 (EMBL:AE001929) Deinococcus radiodurans hydrolase, CbbY/CbbZ/Gph/YirH family DR0729, 259 aa; fasta scores: opt: 227 z-score: 282.6 E(): 3.3e-08; 30.3% identity in 152 aa overlap. Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase 3 3 6 20 [Reporter]SCO4705 (3O2)_PCR/5169 [Gene]SCO4705/4714 NC_003888 SCD31.30, rplB, 50S ribosomal protein L2, len: 278 aa; highly similar to SW:RL2_BACSU (EMBL:D50302) Bacillus subtilis 50S ribosomal protein L2 RplB, 276 aa; fasta scores: opt: 1206 z-score: 1384.9 E(): 0; 62.8% identity in 277 aa overlap. Contains Pfam match to entry PF00181 Ribosomal_L2, Ribosomal Proteins L2 and match to Prosite entry PS00467 Ribosomal protein L2 signature 3 3 6 19 [Reporter]SCO7199 (4K2)_PCR/5168 [Gene]SCO7199/4713 NC_003888 SC1D2.02c, possible membrane protein, len: 131 aa. Similar to Streptomyces coelicolor TR:Q9XAF9(EMBL:AL079356) hypothetical 19.3 kd protein, SC6G9.23 (187 aa), fasta scores opt: 224 z-score: 255.7 E(): 9.1e-07 41.1% identity in 141 aa overlap. Contains a possible membrane spanning hydrophobic domain. 3 3 6 18 [Reporter]SCO6342 (5G2)_PCR/5167 [Gene]SCO6342/4712 NC_003888 SC3A7.10, unknown, len: 135 aa 2 2 13 5 [Reporter]SCO0384 (17N19)_PCR/2788 [Gene]SCO0384/2539 NC_003888 SCF62.10, possible membrane protein, len: 429 aa. Contains a possible membrane spanning hydrophobic domain and a probable coiled-coil from 146 to 176 (31 residues). 3 3 6 17 [Reporter]SCO7346 (6C2)_PCR/5166 [Gene]SCO7346/4711 NC_003888 SC4G10.25c, possible membrane transport protein, len: 484 aa. Highly similar to several including: Streptomyces glaucescens SW:TCMA_STRGA(EMBL:M80674) tetracenomycin C resistance and export protein, TcmA (528 aa), fasta scores opt: 538 z-score: 582.2 E(): 5.8e-25 27.8% identity in 475 aa overlap and Streptomyces coelicolor TR:CAB88188(EMBL:AL352972) putative transport protein (fragment), SCC30.18 (348 aa), fasta scores opt: 1368 z-score: 1475.7 E():0 60.0% identity in 340 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter. 2 2 13 4 [Reporter]SCO1372 (18J19)_PCR/2787 [Gene]SCO1372/2538 NC_003888 SC10A9.14c, possible integral membrane protein, len: 275 aa. Contains possible hydrophobic membrane spanning regions 3 3 6 16 [Reporter]SCO4840 (6O22)_PCR/5165 [Gene]SCO4840/4710 NC_003888 SC5G8.08, possible secreted protein, len: 339aa; N-terminal similar to many eg. TR:Q45633 (EMBL:L13418) maltose permease from Bacillus stearothermophilus (314 aa) fasta scores; opt: 155, z-score: 191.9, E(): 0.0031, 31.5% identity in 127 aa overlap. Contains a possible cleavable N-terminal signal sequence. 2 1 18 15 [Reporter]SCO5755 (8J12)_PCR/892 [Gene]SCO5755/819 NC_003888 SC7C7.10, unknown, len: 126 aa; highly similar to M. tuberculosis TR:O33287 (EMBL:AL008967) possible transcriptional regulator MTV002.10C (Rv2745c) (112 aa), fasta scores; opt: 300 z-score: 454.0 E(): 4.7e-18, 60.5% identity in 86 aa overlap 2 2 13 3 [Reporter]SCP1.245 (19F19)_PCR/2786 [Gene]SCP1.245/2537 NC_003888 SCP1.245, mmfP, hypothetical protein, len: 265aa; previously sequenced and annotated as TR:Q9JN92 (EMBL:AJ276673). Similar to SW:O33611 (IMD_STRCN) inhibition of morphological differentiation protein from Streptomyces cyaneus (Streptomyces curacoi) (277 aa) fasta scores; opt: 329, z-score: 384.0, E(): 6.4e-14, 32.0% identity in 225 aa overlap. Also similar to neighbouring CDS SCP1.235 mmyP (previously sequenced as TR:Q9JN84 (EMBL:AJ276673)) fasta scores; opt: 453, z-score: 556.3, E(): 1.6e-23, 38.9% identity in 234 aa overlap. 2 1 18 14 [Reporter]SCO2635 (9F12)_PCR/891 [Gene]SCO2635/818 NC_003888 SC8E4A.05, probable aminopeptidase, len: 833 aa; similar to SW:AMPN_STRLI (EMBL:L23172) Streptomyces lividans aminopeptidase N (EC 3.4.11.2) PepN, 857 aa; fasta scores: opt: 3002 z-score: 3413.5 E(): 0; 56.5% identity in 832 aa overlap, to TR:O53194 (EMBL:AL021246) Mycobacterium tuberculosis aminopeptidase MTV008.23, 861 aa; fasta scores: opt: 2215 z-score: 2517.8 E(): 0; 50.8% identity in 837 aa overlap and to S. coelicolor St8E4A.13, pepN, 857 aa; fasta scores: opt: 2998 z-score: 2999.1 E(): 0; 55.8% identity in 842 aa overlap. Contains Pfam match to entry PF01433 Peptidase_M1, Peptidase family M1 and match to Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature 2 2 13 2 [Reporter]SCO7508 (20N15)_PCR/2785 [Gene]SCO7508/2536 NC_003888 SCBAC17A6.41c, conserved hypothetical protein, len: 277 aa; similar to many e.g. TR:AAK49792 (EMBL:AF228583) hypothetical protein from Burkholderia pseudomallei (278 aa) fasta scores: opt: 1084, Z-score: 1298.6, 55.636% identity (56.044% ungapped) in 275 aa overlap 2 1 18 13 [Reporter]SCO6239 (10B12)_PCR/890 [Gene]SCO6239/817 NC_003888 SCAH10.04c, possible sigma factor, len: 322 aa; similar to TR:CAB42013 (EMBL:AL049754) Streptomyces coelicolor putative sigma factor, SCH10.04c, 294 aa; fasta scores: opt: 379 z-score: 438.7 E(): 4.6e-17; 34.7% identity in 291 aa overlap and to (EMBL:AL132707) Streptomyces coelicolor SCF51.13c, 298 aa; fasta scores: opt: 833 z-score: 802.7 E(): 0; 44.9% identity in 305 aa overlap 2 2 12 22 [Reporter]SCO1266 (1N11)_PCR/2783 [Gene]SCO1266/2535 NC_003888 2SCG18.13c, fabF2, probable 3-oxoacyl-[acyl-carrier-protein] synthase II, len: 422 aa; highly similar to TR:O54149 (EMBL:AL021409) Streptomyces coelicolor 3-oxoacyl-[acyl-carrier-protein] synthase SC3F7.06c, 407 aa; fasta scores: opt: 1476 z-score: 1594.4 E(): 0; 57.6% identity in 403 aa overlap and to SW:FABF_ECOLI (EMBL:Z34979) Escherichia coli 3-oxoacyl-[acyl-carrier-protein] synthase II (EC 2.3.1.41) (KASII) FabF, 412 aa; fasta scores: opt: 976 z-score: 1056.0 E(): 0; 38.9% identity in 406 aa overlap. Contains Pfam match to entry PF00109 ketoacyl-synt, Beta-ketoacyl synthase 2 1 18 12 [Reporter]SCO4399 (11N8)_PCR/889 [Gene]SCO4399/816 NC_003888 SCD10.31, conserved hypothetical protein, len: 461 aa; similar to TR:Q53958 (EMBL:M80614) Streptomyces coelicolor sporulation-associated protein genes, complete CDS, 473 aa; fasta scores: opt: 666 z-score: 723.4 E(): 8.1e-33; 43.8% identity in 464 aa overlap 2 2 12 21 [Reporter]SCO1491 (2J11)_PCR/2782 [Gene]SCO1491/2534 NC_003888 SC9C5.15c, efp, elongation factor P, len: 188 aa; similar to SW:EFP_BRELA (EMBL:X99289) Brevibacterium lactofermentum elongation factor P Efp, 187 aa; fasta scores: opt: 742 z-score: 903.7 E(): 0; 60.6% identity in 188 aa overlap. Contains Pfam match to entry PF01132 EFP, Elongation factor P (EF-P) and match to Prosite entry PS01275 Elongation factor P signature 2 1 18 11 [Reporter]SCO4634 (12J8)_PCR/888 [Gene]SCO4634/815 NC_003888 SCD82.05c, possible hydrolase, len: 434 aa; similar to SW:ATZC_PSESD (EMBL:AF017572) Pseudomonas sp. N-isopropylammelide isopropyl amidohydrolase (EC 3.5.1.-) AtzC, 403 aa; fasta scores: opt: 292 z-score: 291.5 E(): 8.2e-09; 23.5% identity in 383 aa overlap 2 2 12 20 [Reporter]SCO5626 (3F11)_PCR/2781 [Gene]SCO5626/2533 NC_003888 SC2E1.43, pyrH, uridylate kinase, len: 253 aa; highly similar to many e.g. PYRH_ECOLI uridylate kinase (EC 2.7.4.-) (240 aa), fasta scores; opt: 668 z-score: 968.6 E(): 0, 45.9% identity in 233 aa overlap 2 1 18 10 [Reporter]SCO3235 (13F8)_PCR/887 [Gene]SCO3235/814 NC_003888 SCE29.04c, probable ABC transporter, len: 615aa; similar to many eg. TR:D1035364 (EMBL:AB019513) ABC transporter from Streptomyces coelicolor fasta scores; opt: 783, z-score: 794.3, E(): 0, (42.8% identity in 596 aa overlap). C-terminal half contains Pfam match to entry PF00005 ABC_tran, ABC transporter (score 184.50, E-value 1.7e-51) which itself contains Prosite matches to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). N-terminal half contains Pfam match to entry PF00664 ABC_membrane, ABC transporter transmembrane region., score 129.80, E-value 4.9e-35. Contains probable transmembrane hydrophobic regions. 2 2 12 19 [Reporter]SCO6014 (4B11)_PCR/2780 [Gene]SCO6014/2532 NC_003888 SC1C3.02, possible cationic amino acid transporter, len: 503 aa; similar to many bacterial membrane proteins e.g. M. tuberculosis TR:O05896 EMBL; Z95121 MTCY20B11.28c (495 aa), fasta scores; opt: 1649 z-score: 2116.1 E(): 0, 52.6% identity in 485 aa overlap, and to many eukaryotic transporters e.g. CTR1_HUMAN high-affinity cationic amino acid transporter (629 aa), fasta scores; opt: 897 z-score: 1337.3E(): 0, 35.0% identity in 432 aa overlap. Contains 2x Pfam match to entry aa_permeases PF00324, Amino acid permeases, scores 50.38 and 51.60 2 1 18 9 [Reporter]SCO3428 (14B8)_PCR/886 [Gene]SCO3428/813 NC_003888 SCE9.35c, rpmG, probable 50S ribosomal protein L33, len: 54 aa; similar to many e.g. SW:RL33_ECOLI (EMBL:J01677), rpmG, Escherichia coli 50S ribosomal protein L33 (54 aa), fasta scores; opt: 162 z-score: 270.5 E(): 9.5e-08, 46.9% identity in 49 aa overlap. Contains Pfam match to entry PF00471 Ribosomal_L33, Ribosomal protein L33, score 37.80, E-value 2e-08 2 2 12 18 [Reporter]SCO2070 (5N7)_PCR/2779 [Gene]SCO2070/2531 NC_003888 SC4A10.03, possible membrane protein, len: 382 aa; unknown function, shows weak similarity to parts of many hypothetical proteins e.g. SW:YB43_METJA (EMBL:U67556) Methanococcus jannaschii hypothetical protein (361 aa), fasta scores; opt: 222 z-score: 255.3 E(): 6.7e-07, 24.8% identity in 306 aa overlap. Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF00924 UPF0003, Uncharacterized protein family UPF0003 2 1 18 8 [Reporter]SCO4967 (15N4)_PCR/885 [Gene]SCO4967/812 NC_003888 2SCK31.27, conserved hypothetical protein, len: 293 aa; highly similar to TR:O53430 (EMBL:AL021897) Mycobacterium tuberculosis hypothetical 32.7 kDa protein MTV017.35, 288 aa; fasta scores: opt: 992 z-score: 1173.4 E(): 0; 52.3% identity in 287 aa overlap 2 2 12 17 [Reporter]SCO5829 (6J7)_PCR/2778 [Gene]SCO5829/2530 NC_003888 SC5B8.19c, probable two-component sensor, len: 566 aa; similar to many e.g. CITA_KLEPN P52687 klebsiella pneumoniae sensor kinase (547 aa), fasta scores; opt: 604 z-score: 460.1 E(): 2e-18, 27.1% identity in 554 aa overlap. Contains Pfam match to entry signal PF00512, Signal C terminal domain, score 98.50. Contains possible hydrophobic membrane spanning regions 2 1 18 7 [Reporter]SCO0882 (16J4)_PCR/884 [Gene]SCO0882/811 NC_003888 SCM1.15, unknown, len: 115 aa. Highly similar to another protein of undefined function from Streptomyces coelicolor TR:CAB52051(EMBL:AL109732) hypothetical 14.0 KD protein SC7H2.09c (126 aa), fasta scores opt: 234 z-score: 312.6 E(): 4.6e-10 41.8% identity in 91 aa overlap. 2 1 18 6 [Reporter]SCO3841 (17F4)_PCR/883 [Gene]SCO3841/810 NC_003888 SCH69.11c, unknown, len: 509aa; similar to TR:O86499 (EMBL:AL031232) hypothetical protein from Streptomyces coelicolor (469 aa) fasta scores; opt: 648, z-score: 691.5, E(): 3.5e-31, (41.6% identity in 471 aa overlap) 3 3 6 15 [Reporter]SCO6962 (7K22)_PCR/5164 [Gene]SCO6962/4709 NC_003888 SC6F7.15c, possible glutamine synthetase (EC 6.3.1.2), len: 466 aa. Similar to many glutamine synthetases e.g. Bacillus subtilis SW:GLNA_BACSU(EMBL:M22811) GlnA (443 aa), fasta scores opt: 540 z-score: 593.0 E(): 1.4e-25 28.5% identity in 421 aa overlap. Contains a Pfam match to entry PF00120 gln-synt, Glutamine synthetase 3 3 6 14 [Reporter]SCO5937 (8G22)_PCR/5163 [Gene]SCO5937/4708 NC_003888 SC7H1.07c, unknown, len: 122 aa 3 3 6 13 [Reporter]SCO6057 (9C22)_PCR/5162 [Gene]SCO6057/4707 NC_003888 SC9B1.04c, possible ATP/GTP-binding integral membrane protein, len: 350aa; similar to hypotheticals eg. TR:P71967 (EMBL:Z80225) from Mycobacterium tuberculosis (369 aa) fasta scores; opt: 1129, z-score: 1182.3, E(): 0, (57.8% identity in 325 aa overlap). Contains possible membrane spanning hydrophobic regions. Also contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 3 6 12 [Reporter]SCO1963 (10O18)_PCR/5161 [Gene]SCO1963/4706 NC_003888 SCC54.23c, probable integral membrane export protein, len: 333aa; similar to many egs. TR:O33228 (EMBL:Z98209) hypothetical protein from Mycobacterium tuberculosis (397 aa) fasta scores; opt: 879, z-score: 1040.7, E(): 0, (46.6% identity in 326 aa overlap) and SW:TERC_ALCSP plasmid determinant for resistance to tellurium anions from plasmid pMJ606 of Alcaligenes sp. (346 aa) fasta scores; opt: 249, z-score: 299.8, E(): 2.2e-09, (31.8% identity in 333 aa overlap). Contains several probable membrane spanning hydrophobic regions. 3 3 6 11 [Reporter]SCO4609 (11K18)_PCR/5160 [Gene]SCO4609/4705 NC_003888 SCD39.09, possible peptidase, len: 287 aa; similar to SW:HTPX_MYCTU (EMBL:Z95558) Mycobacterium tuberculosis probable protease HtpX homolog (EC 3.4.24.-), 286 aa; fasta scores: opt: 1208 z-score: 1421.7 E(): 0; 65.4% identity in 283 aa overlap. Contains Pfam match to entry PF01435 Peptidase_M48, Peptidase family M48 and match to Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Also contains possible hydrophobic membrane spanning regions 3 3 6 9 [Reporter]SCO3334 (13C18)_PCR/5158 [Gene]SCO3334/4704 NC_003888 SCE68.32, trpS, probable tryptophanyl-tRNA synthetase, partial CDS, len: >160 aa; similar to many e.g. SW:SYW_ECOLI (EMBL:V00371), trpS, Escherichia coli tryptophanyl-tRNA synthetase (334 aa), fasta scores; opt: 530 z-score: 670.6 E(): 5e-30, 50.0% identity in 162 aa overlap. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature,SCE7.01, trpS, tryptophanyl tRNA synthetase, len: >208aa; similar to many eg. SW:SYW_BACST trpS, tryptophanyl tRNA synthetase from Bacillus stearothermophilus (328 aa) fasta scores; opt: 576, z-score: 685.7, E(): 7.2e-31, (47.8% identity in 203 aa overlap). 3 3 6 8 [Reporter]SCO0130 (14O14)_PCR/5157 [Gene]SCO0130/4703 NC_003888 SCJ21.11, possible beta-lactamase, len; 304 aa; some similarity to S. coelicolor TR:CAB39710 (EMBL:AL049485) putative beta-lactamase SC6A5.26C (310 aa), fasta scores; opt: 197 z-score: 236.5 E(): 8e-06, 28.1% identity in 295 aa overlap, and TR:O34760 (EMBL:AF008220) Bacillus subtilis YtnP (256 aa), fasta scores; opt: 189 z-score: 228.5 E(): 2.2e-05, 25.1% identity in 191 aa overlap and similar to TR:Q9ALW1 (EMBL:AF338729) Plesiomonas sp. M6 methyl parathion hydrolase Mpd, 331 aa; fasta scores: opt: 511 Z-score: 578.8 bits: 115.3 E(): 1.3e-24; 31.61% identity in 310 aa overlap 3 3 6 7 [Reporter]SCO0624 (15K14)_PCR/5156 [Gene]SCO0624/4702 NC_003888 SCF56.08c, possible secreted protein, len: 334 aa. Contains possible N-terminal region signal peptide sequence 2 2 12 16 [Reporter]SCO7264 (7F7)_PCR/2777 [Gene]SCO7264/2529 NC_003888 SC5H1.28c, probable oxidoreductase, len: 305 aa; similar to many e.g. SW:MORA_PSEPU (EMBL:Q02198), morA, Pseudomonas putida morphine 6-dehydrogenase (295 aa), fasta scores; opt: 235 z-score: 281.5 E(): 2.3e-08, 28.1% identity in 274 aa overlap 3 3 6 6 [Reporter]SCO3034 (16G14)_PCR/5155 [Gene]SCO3034/4701 NC_003888 SCE34.15c, whiB, sporulation regulatory protein, len: 87 aa; identical to previously sequenced TR:Q53963 (EMBL:X62287) Streptomyces coelicolor regulatory protein WhiB, 87 aa also highly similar to TR:Q9XAL5 (EMBL:AL079355) Streptomyces coelicolor probable transcriptional regulator SC4C6.25, 81 aa; fasta scores: opt: 336 z-score: 473.5 E(): 7.3e-21; 61.1% identity in 72 aa overlap 2 2 12 15 [Reporter]SCO4758 (8B7)_PCR/2776 [Gene]SCO4758/2528 NC_003888 SC6G4.36c, similar to hypothetical proteins e.g. M. tuberculosis TR:O53284 (EMBL:AL021287) MTV012.52c (358 aa) , fasta scores; opt: 545 z-score: 329.5 E(): 4.1e-11, 36.2% identity in 354 aa overlap 3 3 6 5 [Reporter]SCO0551 (17C14)_PCR/5154 [Gene]SCO0551/4700 NC_003888 SCF11.31c, possible histidine kinase protein, len: 442 aa; similar to TR:O50497 (EMBL:AL020958) Streptomyces coelicolor two-component system sensor, 481 aa; fasta scores: opt: 401 z-score: 451.3 E(): 9e-18; 30.2% identity in 367 aa overlap. Contains Pfam matches to entries PF00512 signal, histidine kinase and PF00672 DUF5, domain found in bacterial signal proteins. Contains possible N-terminal signal peptide sequence 2 1 18 5 [Reporter]SCO1070 (18B4)_PCR/882 [Gene]SCO1070/809 NC_003888 SCG22.16c, possible two component system response regulator, len: 221 aa; similar to TR:O69816 (EMBL:AL023496) Streptomyces coelicolor two component regulator SC1A6.11, 224 aa; fasta scores: opt: 872 z-score: 1006.3 E(): 0; 64.8% identity in 216 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family. Contains also a possible helix-turn-helix motif at residues 170..191 (+2.64 SD) 2 2 12 14 [Reporter]SCO7809 (9N3)_PCR/2775 [Gene]SCO7809/2527 NC_003888 SC8E7.06, possible TetR-family transcriptional regulator, len: 210 aa. Similar to several other members of the TetR-family e.g. Streptomyces glaucescens SW:TCMR_STRGA (EMBL:M80674) tetracenomycin C transcriptional repressor, TcmR (226 aa), fasta scores opt: 187 z-score: 235.6 E(): 1.2e-05 26.4% identity in 197 aa overlap and Streptomyces coelicolor TR:CAB88457 (EMBL:AL353815) putative TetR-family transcriptional regulator, SCD6.28 (215 aa), fasta scores opt: 239 z-score: 298.6 E(): 3.7e-09 32.0% identity in 178 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 36..57 (+5.84 SD). 2 1 18 4 [Reporter]SCP1.132c (18N24)_PCR/881 [Gene]SCP1.132c/808 NC_003888 SCP1.132c, unknown, len: 178aa; 2 2 12 13 [Reporter]SCO6264 (10J3)_PCR/2774 [Gene]SCO6264/2526 NC_003888 SCAH10.29, reductase orfX, len: 262 aa; identical to previously sequenced TR:O86853 (EMBL:AJ007731) Streptomyces coelicolor 3-ketoacyl-ACP/CoA reductase, 244 aa and to SW:VER1_ASPPA (EMBL:M91369) Aspergillus parasiticus versicolor reductase (EC 1.1.-.-)(ver-1) 262 aa; fasta scores opt: 571 z-score: 634.6 E(): 5.7e-28; 40.9% identity in 259 aa overlap. Contains match to Pfam entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 2 1 18 3 [Reporter]SCP1.212 (19J24)_PCR/880 [Gene]SCP1.212/807 NC_003888 SCP1.212, possible bifunctional undecaprenyl pyrophosphate synthetase/phytoene synthase, len: 564aa; C-terminal similar to many undecaprenyl pyrophosphate synthetases eg. SW:Q47675 (UPPS_ECOLI) undecaprenyl pyrophosphate synthetase (EC 2.5.1.31) from Escherichia coli (253 aa) blastp scores; Identities = 94/226 (41%), Positives = 132/226 (58%). N-terminal similar to many phytoene synthases eg. SW:P54977 (CRTB_STRGR) phytoene synthase (EC 2.5.1.-) from Streptomyces griseus (342 aa) blastp scores; Identities = 82/298 (27%), Positives = 133/298 (44%). C-terminal region contains Pfam match to entry PF01255 UPP_synthetase, Putative undecaprenyl diphosphate synthase. Contains a TTA encoded leucine at residue 11, possible target for bldA regulation. 2 2 12 11 [Reporter]SCO4774 (12B3)_PCR/2772 [Gene]SCO4774/2525 NC_003888 SCD63.06, probable glycerol phosphate dehydrogenase, len: 568 aa; similar to SW:GLPD_BACSU (EMBL:M34393) Bacillus subtilis aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) GlpD, 555 aa; fasta scores: opt: 815 z-score: 910.2 E(): 0; 32.1% identity in 546 aa overlap. Contains Pfam match to entry PF01224 FAD_Gly3P_dh, FAD-dependent glycerol-3-phosphate dehydrogenase and match to Prosite entry PS00978 FAD-dependent glycerol-3-phosphate dehydrogenase signature 2 2 1 18 2 [Reporter]SCO2815 (20B24)_PCR/879 [Gene]SCO2815/806 NC_003888 SCBAC17F8.06, possible TetR-family transcriptional regulator, len: 232 aa: similar to many hypothetical regulators eg. TR:Q9RWD4 (EMBL:AE001929) TetR-family transcriptional regulator from Deinococcus radiodurans (229 aa) fasta scores; opt: 164, Z-score: 191.6, 34.416% identity (36.054% ungapped) in 154 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. 2 2 12 10 [Reporter]SCO2838 (12N23)_PCR/2771 [Gene]SCO2838/2524 NC_003888 SCE20.12c, possible secreted endoglucanase, len: 332 aa. Similar to many including: Streptomyces halstedii SW:GUN1_STRHA(EMBL:Z12157) endoglucanase 1 precursor (EC 3.2.1.4) (321 aa), fasta scores opt: 613 z-score: 677.4 E(): 2.4e-30 37.6% identity in 343 aa overlap and Mycobacterium tuberculosis TR:O53607(EMBL:AL021428) putative cellulase (380 aa), fasta scores opt: 796 z-score: 876.5 E():0 44.3% identity in 298 aa overlap. Note that codon 10 maybe the true initiation codon. Contains a Pfam match to entry PF01341 Glyco_hydro_6, Glycosyl hydrolases and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains a possible N-terminal signal sequence. 2 1 17 22 [Reporter]SCO1128 (1B20)_PCR/877 [Gene]SCO1128/805 NC_003888 2SCG38.21, possible integral membrane protein, len: 302 aa; similar to TR:Q9RJN5 (EMBL:AL132991) Streptomyces coelicolor putative integral membrane protein SCF55.33, 287 aa; fasta scores: opt: 590 z-score: 654.2 E(): 5.4e-29; 42.4% identity in 278 aa overlap. Contains possible hydrophobic membrane spanning regions 2 2 12 9 [Reporter]SCO0842 (13J23)_PCR/2770 [Gene]SCO0842/2523 NC_003888 SCF43A.32, phr, probable deoxyribodipyrimidine photolyase, len: 458 aa; highly similar to many deoxyribodipyrimidine photolyases e.g. SW:PHR_STRGR (EMBL:X15060), phr, Streptomyces griseus deoxyribodipyrimidine photolyases (455 aa), fasta scores; opt: 2043 z-score: 1879.8 E(): 0, 67.1% identity in 459 aa overlap. Contains Pfam match to entry PF00875 DNA_photolyase, DNA photolyase, PS00394 DNA photolyases class 1 signature 1 and PS00691 DNA photolyases class 1 signature 2 2 1 17 21 [Reporter]SCO0435 (2N16)_PCR/876 [Gene]SCO0435/804 NC_003888 SCF51A.13, malY, aminotransferase, len: 401 aa. Highly similar to Escherichia coli SW:MALY_ECOLI (EMBL; M60722) MalY protein (EC 2.6.1.-) (390 aa), fasta scores opt: 1577 z-score: 1814.7 E():0 58.5% identity in 386 aa overlap 2 2 12 8 [Reporter]SCO1036 (14F23)_PCR/2769 [Gene]SCO1036/2522 NC_003888 SCG20A.16, probable phosphotriesterase-family protein, len: 302 aa; similar to SW:PHP_ECOLI (EMBL:U18997) Escherichia coli phosphotriesterase homology protein Php, 292 aa; fasta scores: opt: 319 z-score: 370.9 E(): 3.5e-13; 28.0% identity in 286 aa overlap. Contains Pfam match to entry PF02126 PTE, Phosphotriesterase family and match to Prosite entry PS01323 Phosphotriesterase family signature 2 2 1 17 20 [Reporter]SCO6822 (3J16)_PCR/875 [Gene]SCO6822/803 NC_003888 SC1A2.31, possible integral membrane efflux protein, len: 521 aa. Highly similar to many members of the drug resistance translocase family including: Streptomyces virginiae TR:Q9XE01 (EMBL:AB019519) virginiamycin S resistance protein, VarS (518 aa), fasta scores opt: 1230 z-score: 1294.4 E():0 40.7% identity in 523 aa overlap and Streptomyces coelicolor TR:Q9Z563 (EMBL:AL035569) putative integral membrane efflux protein, SC8D9.28C (521 aa), fasta scores opt: 1072 z-score: 1128.8 E():0 37.7% identity in 515 aa overlap. Contains a Prosite hit to PS00216 Sugar transport proteins signature 1 and a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter. Also contains a possible N-terminal signal sequence and multiple possible membrane spanning hydrophobic domains. 2 2 12 7 [Reporter]SCO0496 (15B23)_PCR/2768 [Gene]SCO0496/2521 NC_003888 SCF34.15c, probable iron-siderophore permease transmembrane protein, len: 368 aa; similar to SW:FEPG_ECOLI (EMBL:X57471), FepG, Escherichia coli ferric enterobactin transport protein (330 aa), fasta scores; opt: 741 z-score: 794.3 E(): 0, 38.7% identity in 326 aa overlap and to many other iron-siderophore permeases. Similar to others from S. coelicolor e.g. SCI51.26c (EMBL:AL109848) S. coelicolor probable iron-siderophore uptake system transmembrane component (375 aa) (45.9% identity in 333 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF01032 FecCD_family, FecCD transport family 2 1 17 19 [Reporter]SCO6272 (4F16)_PCR/874 [Gene]SCO6272/802 NC_003888 SC2C4.02, possible secreted FAD-binding protein, len: 550 aa; similar to TR:Q9ZAR8 (EMBL:U80222) Streptomyces peucetius ceasius DnrW, 485 aa; fasta scores: opt: 1323 z-score: 1315.4 E(): 0; 45.3% identity in 470 aa overlap. Contains Pfam match to entry PF01565 FAD_binding_4, FAD binding domain. Also contains possible N-terminal region signal peptide sequence 2 2 12 6 [Reporter]SCO2979 (16N19)_PCR/2767 [Gene]SCO2979/2520 NC_003888 SCE50.07, possible integral membrane transport protein, len: 427 aa; N-terminal and C-terminal regions both similar to TR:Q55472 (EMBL:D64006) Synechocystis sp. membrane bound sugar transport protein, 279 aa; fasta scores: opt: 307 z-score: 333.7 E(): 3.8e-11; 26.5% identity in 412 aa overlap. Contains Pfam match in C-terminal region to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match also in C-terminal region to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains possible N-terminal region signal peptide sequence and also possible hydrophobic membrane spanning regions 2 1 17 18 [Reporter]SCO2396 (5B16)_PCR/873 [Gene]SCO2396/801 NC_003888 SC4A7.24c, hypothetical protein, len: 139 aa; similar to TR:O68390 (EMBL:AF036166) Xanthomonas campestris organic hydroperoxide resistance protein Ohr, 142 aa; fasta scores: opt: 478 z-score: 578.1 E(): 8e-25; 55.2% identity in 134 aa overlap and to TR:P70736 (EMBL:Y09102) Acinetobacter sp. hypothetical 15.2 kD protein, 143 aa; fasta scores: opt: 442 z-score: 535.7 E(): 1.9e-22; 53.3% identity in 137 aa overlap 2 1 17 17 [Reporter]SCO6543 (6N12)_PCR/872 [Gene]SCO6543/800 NC_003888 SC5C7.28c, unknown, len: 81 aa 2 2 12 5 [Reporter]SCO4863 (17J19)_PCR/2766 [Gene]SCO4863/2519 NC_003888 SCK20.04, unknown, len: 140 aa 2 2 12 4 [Reporter]SCO3159 (18F19)_PCR/2765 [Gene]SCO3159/2518 NC_003888 SCE87.10, possible membrane protein, len: 353 aa. Weakly similar to Salmonella typhimurium SW:CBIM_SALTY (EMBL:L12006) cobalamin biosynthesis protein CbiM (245 aa), fasta scores opt: 224 z-score: 251.7 E(): 1.2e-06 32.8% identity in 180 aa overlap. Also similar to Methanobacterium thermoautotrophicum TR: O27742 (EMBL:AE000928) cobalamin biosynthesis protein M (217 aa), fasta scores opt: 495 z-score: 544.6 E(): 5.9e-23 41.4% identity in 220 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence 2 2 12 3 [Reporter]SCO1317 (19B19)_PCR/2764 [Gene]SCO1317/2517 NC_003888 SCBAC36F5.28c, conserved hypothetical protein, len: 145 aa; similar to TR:Q9RSU3 (EMBL:AE002039) Deinococcus radiodurans hypothetical 196.9 kDa protein DR2030, 159 aa; fasta scores: opt: 222 Z-score: 270.7 bits: 56.2 E(): 1.8e-07; 36.296% identity in 135 aa overlap 2 2 12 2 [Reporter]SCO5104 (20J15)_PCR/2763 [Gene]SCO5104/2516 NC_003888 SCBAC28G1.30, hypothetical protein, len: 880 aa; similar to many Streptomyces coelicolor hypothetical proteins, e.g. TR:Q9KYN1 (EMBL:AL356592) hypothetical 84.7 kDa protein SC9H11.08, 802 aa; fasta scores: opt: 1062 Z-score: 1155.9 bits: 224.9 E(): 9.1e-57; 38.902% identity in 856 aa overlap. Contains 2x Pfam matches, Pfam match to entry PF01590 GAF, GAF domain, to entry PF00989 PAS, PAS domain and to entry PF02518 HATPase_c, Histidine kinase-, DNA gyrase B-, phytochrome-like ATPase 2 2 11 22 [Reporter]SCO1262 (1J11)_PCR/2761 [Gene]SCO1262/2515 NC_003888 2SCG18.09c, probable gntR-family transcriptional regulator, len: 253 aa; similar to TR:Q9RKT6 (EMBL:AL132824) Streptomyces coelicolor putative transcriptional regulator SCAH10.21, 245 aa; fasta scores: opt: 306 z-score: 352.0 E(): 4e-12; 32.0% identity in 241 aa overlap and to SW:FARR_ECOLI (EMBL:X15790) Escherichia coli fatty acyl responsive regulator (P30 protein) FarR, 240 aa; fasta scores: opt: 204 z-score: 238.2 E(): 8.7e-06; 26.2% identity in 233 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and possible helix-turn-helix motif at residues 36..57 (+3.93 SD) 2 2 11 21 [Reporter]SCO6819 (2F11)_PCR/2760 [Gene]SCO6819/2514 NC_003888 SC1A2.28c, aroA, 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19), len: 440 aa. Highly similar to many e.g. Escherichia coli SW:AROA_ECOLI (EMBL:X00557) 3-phosphoshikimate 1-carboxyvinyltransferase (427 aa), fasta scores opt: 990 z-score: 1069.3 E():0 41.1% identity in 421 aa overlap. Contains a Pfam match to entry PF00275 EPSP_synthase, EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) and Prosite hits to PS00885 EPSP synthase signature 2. 2 2 11 20 [Reporter]SCO1487 (3B11)_PCR/2759 [Gene]SCO1487/2513 NC_003888 SC9C5.11c, pyrB, aspartate carbamoyltransferase, len: 326 aa; similar to SW:PYRB_PSEPU (EMBL:M97253) Pseudomonas putida aspartate carbamoyltransferase (EC 2.1.3.2) PyrB, 334 aa; fasta scores: opt: 902 z-score: 1064.2 E(): 0; 47.3% identity in 311 aa overlap. Contains 2x Pfam matches to entry PF00185 OTCace, Aspartate/ornithine carbamoyltransferase and match to Prosite entry PS00097 Aspartate and ornithine carbamoyltransferases signature 2 2 11 19 [Reporter]SCO2249 (4N7)_PCR/2758 [Gene]SCO2249/2512 NC_003888 SC1G2.11, unknown, len: 76 aa. 2 2 11 18 [Reporter]SCO2203 (5J7)_PCR/2757 [Gene]SCO2203/2511 NC_003888 SC3H12.11, hypothetical protein, len: 192 aa; identical to previously sequenced TR:CAB58285 (EMBL:AL121850) Streptomyces coelicolor hypothetical 20.8 kD protein SCC78.04 and similar to SW:YMU5_STRCM (EMBL:L10064) Streptomyces cinnamonensis hypothetical 25.0 kD protein in MutA 5'region (ORF-D), 237 aa; fasta scores: opt: 114 z-score: 145.0 E(): 1.1; 31.1% identity in 151 aa overlap 2 2 11 17 [Reporter]SCO2053 (6F7)_PCR/2756 [Gene]SCO2053/2510 NC_003888 SC4G6.22c, hisC1, histidinol-phosphate aminotransferase, len: 369aa; previously sequenced therefore identical to SW:HIS8_STRCO. Contains Pfam match to entry PF00222 aminotran_2, Aminotransferases class-II and Prosite match to PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. 2 2 11 16 [Reporter]SCO7268 (7B7)_PCR/2755 [Gene]SCO7268/2509 NC_003888 SC5H1.24c, add, probable adenosine deaminase, len: 359 aa; similar to many e.g. SW:ADD_ECOLI (EMBL:M59033), Add, Escherichia coli adenosine deaminase (333 aa), fasta scores; opt: 369 z-score: 434.3 E(): 7.2e-17, 32.8% identity in 338 aa overlap. Weakly similar to TR:O86737 (EMBL:AL031035) S.coelicolor possible adenosine deaminase (387 aa) (28.9% identity in 342 aa overlap). Contains Pfam match to entry PF00962 A_deaminase, Adenosine/AMP deaminase, score 220.40, E-value 2.6e-62 2 2 11 15 [Reporter]SCO5579 (8N3)_PCR/2754 [Gene]SCO5579/2508 NC_003888 SC7A1.23c, possible transmembrane protein, len: 485aa; similar to many putative and hypothetical proteins eg. EMBL:E1312898 putative transmembrane transport protein from Streptomyces coelicolor (476 aa) fasta scores; opt: 482, z-score: 311.0, E(): 5e-10, (26.4% identity in 473 aa overlap) Also weakly similar to SW:BETT_ECOLI high-affinity choline transport protein from Escherichia coli (677 aa) opt: 74, z-score: 196.3, E(): 0.0012, (23.0% identity in 283 aa overlap). Contains several possible transmembrane regions. Also similar to SC1C2.05c (EMBL:AL031124) possible transmembrane transport protein from Streptomyces coelicolor (476 aa) fasta scores; opt: 482, z-score: 215.0, E(): 3.4e-07, (26.4% identity in 473 aa overlap) 2 2 11 14 [Reporter]SCO2657 (9J3)_PCR/2753 [Gene]SCO2657/2507 NC_003888 SC8E4A.27, possible ROK-family transcriptional regulatory protein, len: 409 aa; similar to TR:O69941 (EMBL:AL023862) Streptomyces coelicolor hypothetical ROK family protein (fragment) SC3F9.01, 403 aa; fasta scores: opt: 456 z-score: 480.7 E(): 2.3e-19; 30.8% identity in 396 aa overlap and to SW:XYLR_CALSR (EMBL:L18965) Caldicellulosiruptor sp. putative xylose repressor XylR, 399 aa; fasta scores: opt: 381 z-score: 402.9 E(): 5e-15; 25.2% identity in 361 aa overlap. Contains Pfam match to entry PF00325 crp, Bacterial regulatory proteins, crp family and Pfam match to entry PF00480 ROK, ROK family. Contains also possible helix-turn-helix motif at residues 32..53 (+5.09 SD) 2 2 11 13 [Reporter]SCO5119 (10F3)_PCR/2752 [Gene]SCO5119/2506 NC_003888 SC9E12.04, probable peptide transport system integral membrane protein, len: 305 aa; similar to SW:OPPC_BACSU (EMBL:X56347) Bacillus subtilis oligopeptide transport system permease protein OppC, 305 aa; fasta scores: opt: 670 z-score: 745.0 E(): 0; 35.5% identity in 296 aa overlap and to TR:P72406 (EMBL:U68036) Streptomyces coelicolor SpoOKB homologue BldKA, 335 aa; blastp scores: Score = 382 (134.5 bits), Expect = 9.1e-41, Sum P(2) = 9.1e-41; Identities = 88/231 (38%), Positives = 134/231 (58%). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and possible hydrophobic membrane spanning regions 2 2 11 12 [Reporter]SCO2259 (11B3)_PCR/2751 [Gene]SCO2259/2505 NC_003888 SCC75A.05c, possible multi-domain regulatory protein, len: 1334 aa. Similar in parts to many regulators. Similar in the N-terminus to Streptomyces coelicolor SW:AFSR_STRCO (EMBL:D90155) regulatory protein AfsR (993 aa), fasta scores opt: 524 z-score: 356.0 E(): 1.9e-12 37.0% identity in 316 aa overlap. Similar in the C-terminus to many other proposed regulators e.g. Mycobacterium tuberculosis TR:O53720 (EMBL:AL021931) transcriptional regulatory protein (1085 aa), fasta scores opt: 809 z-score: 541.8 E(): 8.3e-23 34.1% identity in 883 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). Also contains a highly degenerate repeat region with 16x PA(P/S)G and 5x APG. This repeat region also has a notably higher GC content (83.4%). Contains probable coiled-coils between residues 867 to 904 (38 residues) and 1093 to 1121 (29 residues). 2 2 11 11 [Reporter]SCO4434 (11N23)_PCR/2750 [Gene]SCO4434/2504 NC_003888 SCD6.12, probable LysR-family transcriptional regulator, len: 298 aa; similar to TR:Q9X9U9 (EMBL:Y18817) Streptomyces coelicolor A3(2) transcriptional regulator of LysR family, 307 aa; fasta scores: opt: 736 z-score: 859.4 E(): 0; 43.6% identity in 303 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family, match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature and possible helix-turn-helix motif at residues 18..39 (+4.94 SD) 2 2 11 10 [Reporter]SCO2834 (12J23)_PCR/2749 [Gene]SCO2834/2503 NC_003888 SCE20.08c, possible membrane protein, len: 383 aa; C-terminal region similar to TR:Q9KIM1 (EMBL:AF230489) Streptomyces coelicolor F42A (putative integral membrane protein) or SCK7.11c, 312 aa; fasta scores: opt: 467 Z-score: 441.6 E(): 5.8e-17; 31.229% identity in 301 aa overlap. Contains possible membrane spanning hydrophobic domains 2 2 11 9 [Reporter]SCO0159 (13F23)_PCR/2748 [Gene]SCO0159/2502 NC_003888 SCJ1.08 putative ECF sigma factor, len: 322 aa, similar to many e.g. S. coelicolor TR:CAB44418 (EMBL:AL078610) putative ECF sigma factor SCH35.09 (223 aa) fasta scores, opt: 656 z-score: 777.0 E(): 0 64.6% identity in 175 aa overlap and Mycobacterium tuberculosis SIGI protein TR:O50445 (EMBL; AL010186)(290 aa), fasta scores opt: 385 z-score: 458.0 E(): 3.7e-18 30.0% identity in 290 aa overlap. Contains a Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily). Possible helix-turn-helix motif between 142-163 aa, score +4.11 SD 2 2 11 8 [Reporter]SCO1915 (14B23)_PCR/2747 [Gene]SCO1915/2501 NC_003888 SCI7.33, unknown, len: 242aa; 2 2 11 7 [Reporter]SCO3659 (15N19)_PCR/2746 [Gene]SCO3659/2500 NC_003888 SCH10.37c, hypothetical protein, len: 178 aa; unknown function, has unusally low percentage G+C content 4 3 8 8 [Reporter]SCO0045 (14H18)_PCR/5705 [Gene]SCO0045/5199 NC_003888 SCJ4.26c, unknown, len: 42 aa 4 3 8 7 [Reporter]SCO0267 (15D18)_PCR/5704 [Gene]SCO0267/5198 NC_003888 SCF1.09, possible hydrolase, len: 258 aa; similar to many e.g. TR:O67982 (EMBL:AF003947) 3-oxoadipate enol-lactone hydrolase from Rhodococcus opacus (400 aa) fasta scores; opt: 259, z-score: 300.,1 E(): 2.4e-09, (31.5% identity in 251 aa overlap). Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 4 3 8 6 [Reporter]SCO0487 (16P14)_PCR/5703 [Gene]SCO0487/5197 NC_003888 SCF34.06, possible MarR-family transcriptional regulator, len: 168 aa; shows weak similarity to TR:Q53002 (EMBL:U51475), MucS, Rhizobium meliloti exopolysaccharide synthesis regulatory protein (190 aa), fasta scores; opt: 126 z-score: 160.1 E(): 0.15, 24.8% identity in 145 aa overlap and to hypothetical transcriptional regulators. Contains Pfam match to entry PF01047 MarR, MarR family 4 3 8 5 [Reporter]SCO1814 (17L14)_PCR/5702 [Gene]SCO1814/5196 NC_003888 SCI28.08c, inhA, probable enoyl-(acyl-carrier-protein) reductase, len: 269 aa; similar to many e.g. SW:INHA_MYCSM enoyl-(acyl-carrier-protein) reductase involved from Mycobacterium smegmatis (269 aa) fasta scores; opt: 740, z-score: 852.1, E(): 0, (51.3% identity in 263 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 4 3 8 4 [Reporter]SCO0367 (18H14)_PCR/5701 [Gene]SCO0367/5195 NC_003888 SCF41.26, putative membrane protein, len: 185 aa. A low level of similarity to Streptomyces coelicolor TR:O54175 (EMBL; AL021411) hypothetical 24.6 KD protein SC7H1.16 (257 aa), fasta scores opt: 190 z-score: 226.1 E(): 3.1e-05 35.1% identity in 188 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 8 3 [Reporter]SCP1.158 (19D14)_PCR/5700 [Gene]SCP1.158/5194 NC_003888 SCP1.158, unknown, len: 483aa; 4 3 8 2 [Reporter]SCO1873 (20L10)_PCR/5699 [Gene]SCO1873/5193 NC_003888 SCI39.20, probable acetyltransferase, len: 156 aa; similar to TR:Q9SKZ7 (EMBL:AC006223) Arabidopsis thaliana putative alanine acetyl transferase AT2G32020, 183 aa; fasta scores: opt: 205 Z-score: 253.5 bits: 53.3 E(): 1.6e-06; 31.210% identity in 157 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 4 3 7 22 [Reporter]SCO4216 (1L6)_PCR/5697 [Gene]SCO4216/5192 NC_003888 2SCD46.30c, unknown, len: 69 aa 4 3 7 19 [Reporter]SCO6495 (4P2)_PCR/5694 [Gene]SCO6495/5191 NC_003888 SC1E6.04c, probable dehydrogenase, len: 320 aa; similar to Mycobacterial F420-dependent glucose-6-phosphate dehydrogenases e.g. M. smegmatis TR:O68447 (EMBL:AF041061) F420-dependent glucose-6-phosphate dehydrogenase (336 aa), fasta scores; opt: 499 z-score: 362.9 E(): 6.2e-13, 33.2% identity in 331 aa overlap 4 3 7 18 [Reporter]SCO6846 (5L2)_PCR/5693 [Gene]SCO6846/5190 NC_003888 SC3D9.14c, unknown, len: 107 aa. 4 3 7 17 [Reporter]SCO6631 (6H2)_PCR/5692 [Gene]SCO6631/5189 NC_003888 SC4G2.05, putative membrane protein, len: 291 aa; similar to TR:AAK74714 (EMBL:AE007366) Streptococcus pneumoniae conserved hypothetical protein SP0558, 271 aa; fasta scores: opt: 522 Z-score: 580.2 E(): 1.1e-24; 37.008% identity in 254 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 7 16 [Reporter]SCO0304 (7D2)_PCR/5691 [Gene]SCO0304/5188 NC_003888 SC5G9.13c, unknown, len: 122 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 4 3 7 15 [Reporter]SCO6968 (7P22)_PCR/5690 [Gene]SCO6968/5187 NC_003888 SC6F7.21, probable long-chain-fatty-acid-CoA ligase, len: 511 aa. Similar to several including: Bacillus subtilis SW:LCFA_BACSU (EMBL:Z75208) long-chain-fatty-acid-CoA ligase (EC 6.2.1.3) (560 aa), fasta scores opt: 877 z-score: 981.0 E():0 35.4% identity in 534 aa overlap and Archaeoglobus fulgidus TR:O28502 (EMBL:AE000980) long-chain-fatty-acid-CoA ligase (569 aa), fasta scores opt: 971 z-score: 1085.9 E():0 38.1% identity in 525 aa overlap. Contains a Prosite hit to PS00455 Putative AMP-binding domain signature and a Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme. 4 3 7 14 [Reporter]SCO7195 (8L22)_PCR/5689 [Gene]SCO7195/5186 NC_003888 SC8A11.23c, unknown, len: 88 aa 4 3 7 13 [Reporter]SCO6062 (9H22)_PCR/5688 [Gene]SCO6062/5185 NC_003888 SC9B1.09, ABC transporter ATP-binding subunit, len: 386aa; similar to many eg. TR:O69724 (EMBL:AL022121) putative ABC transporter ATP-binding subunit from Mycobacterium tuberculosis (376 aa) fasta scores; opt: 1147, z-score: 1185.4, E(): 0, (51.2% identity in 371 aa overlap) and SW:PROV_BACSU glycine betaine/L-proline transport ATP-binding protein (404 aa) fasta scores; opt: 849, z-score: 879.1, E(): 0, (39.8% identity in 359 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. 4 3 7 12 [Reporter]SCO2429 (10D22)_PCR/5687 [Gene]SCO2429/5184 NC_003888 SCC42.10c, possible secreted protein, len: 218 aa. Contains possible N-terminal region signal peptide sequence 4 3 7 11 [Reporter]SCO4614 (11P18)_PCR/5686 [Gene]SCO4614/5183 NC_003888 SCD39.14c, conserved hypothetical protein, len: 162 aa; highly similar to TR:O53763 (EMBL:AL021942) Mycobacterium tuberculosis hypothetical 18.1 kDa protein MTV039.04c, 163 aa; fasta scores: opt: 705 z-score: 803.7 E(): 0; 64.4% identity in 163 aa overlap 4 3 7 10 [Reporter]SCO2919 (12L18)_PCR/5685 [Gene]SCO2919/5182 NC_003888 SCE19A.19c, hypothetical protein, len: 114 aa; unknown function, probable CDS suggested by probable CDS suggested by GC frameplot, positional base preference and amino acid composition 4 3 7 9 [Reporter]SCO3317 (13H18)_PCR/5684 [Gene]SCO3317/5181 NC_003888 SCE68.15c, possible uroporphyrin-III C-methyltransferase/uroporphyrinogen-III synthase, len: 565 aa; similar to many e.g. SW:HEM4_CLOJO (EMBL:D28503), hemD, Clostridium josui porphyrin biosynthesis probable bifunctional protein (504 aa), fasta scores; opt: 519 z-score: 562.5 E(): 5.2e-24, 29.8% identity in 533 aa overlap. The C-terminal half is similar to the monofunctional SW:HEM4_SYNP7 (EMBL:X70966) Synechococcus sp. uroporphyrinogen-III synthase (264 aa) (29.7% identity in 263 aa overlap). May be involved in cysteine biosynthesis and/or porphyrin biosynthesis. Contains Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases and PS00839 Uroporphyrin-III C-methyltransferase signature 1 4 3 7 8 [Reporter]SCO0969 (14D18)_PCR/5683 [Gene]SCO0969/5180 NC_003888 SCM11.24c, possible reductase, len: 207 aa; similar to SW:F4RE_METJA (EMBL:U67591) Methanococcus jannaschii putative F420-dependent NADP reductase (EC 1.-.-.-) MJ1501, 223 aa; fasta scores: opt: 169 z-score: 190.2 E(): 0.0032; 27.0% identity in 200 aa overlap and to SCM11.23c, 207 aa; fasta scores: opt: 761 z-score: 742.1 E(): 0; 60.3% identity in 204 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 3 7 7 [Reporter]SCO1910 (15P14)_PCR/5682 [Gene]SCO1910/5179 NC_003888 SCI7.28c, possible alanine-rich protein, len: 165 aa; contains 3 x H**H*KAGRA* repeat region. Largely hydrophilic. Contains possible hydrophobic membrane spanning region at C-terminal domain 4 3 7 6 [Reporter]SCO0172 (16L14)_PCR/5681 [Gene]SCO0172/5178 NC_003888 SCJ1.21, hypothetical protein, len: 152 aa. Similar to many hypothetical proteins including: Mycobacterium tuberculosis TR:O53472 (EMBL; AL021899) hypothetical 31.5 KD protein MTV018.13C (294 aa), fasta scores opt: 248 z-score: 298.8 E(): 2.8e-09 35.6% identity in 160 aa overlap and Methanobacterium thermoautotrophicum TR:O27074 (EMBL; AE000872) conserved protein (143 aa), fasta scores opt: 195 z-score: 241.7 E(): 4.2e-06 32.4% identity in 142 aa overlap. Contains a Pfam match to entry PF00582 Usp, Universal stress protein family. 4 3 7 5 [Reporter]SCO4951 (17H14)_PCR/5680 [Gene]SCO4951/5177 NC_003888 2SCK31.11c, probable aldoketoreductase, len: 345 aa; similar to TR:Q9XC70 (EMBL:AF147704) Streptomyces fradiae NDP-hexose 2,3-enoyl reductase TylCII, 329 aa; fasta scores: opt: 726 z-score: 830.3 E(): 0; 41.3% identity in 320 aa overlap. Contains 2x Pfam matches to entry PF00248 aldo_ket_red, Aldo/keto reductase family 4 3 7 4 [Reporter]SCO0805 (18D14)_PCR/5679 [Gene]SCO0805/5176 NC_003888 SCF43.16c, possible prolyl aminopeptidase, len: 433 aa. Highly similar to several peptidases including: Aeromonas sobria SW:PIP_AERSO(EMBL:D30714) proline iminopeptidase (EC 3.4.11.5) (prolyl aminopeptidase) (424 aa), fasta scores opt: 954 z-score: 1113.1 E():0 48.6% identity in 428 aa overlap and Hafnia alvei TR:P94800(EMBL:D61383) prolyl aminopeptidase (427 aa), fasta scores opt: 1208 z-score: 1408.6 E():0 47.2% identity in 432 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 4 3 7 3 [Reporter]SCP1.253 (19P10)_PCR/5678 [Gene]SCP1.253/5175 NC_003888 SCP1.253, unknown, len: 205aa; 4 3 7 2 [Reporter]SCO1975 (20H10)_PCR/5677 [Gene]SCO1975/5174 NC_003888 SC3C9.10c, unknown, len: 210 aa 4 3 6 22 [Reporter]SCO5392 (1H6)_PCR/5675 [Gene]SCO5392/5173 NC_003888 2SC6G5.36c, possible ABC transporter integral membrane subunit, len: 289aa; similar to many membrane proteins of eg. TR:O86630 (EMBL:AL031155) probable integral membrane protein from Streptomyces coelicolor (279 aa) fasta scores; opt: 424, z-score: 479.6, E(): 3.1e-19, 33.9% identity in 274 aa overlap. Contains possible membrane-spanning hydrophobic regions and lies immediately downstream of a possible ABC transporter ATP-binding subunit. 4 3 6 21 [Reporter]SCO4383 (2D6)_PCR/5674 [Gene]SCO4383/5172 NC_003888 SCD10.15, 4CL-2, 4-coumarate:CoA ligase, len: 522 aa; similar to many eukaryotic ones, e.g. TR:O81139 (EMBL:AF041049) Populus tremuloides 4-coumarate:CoA ligase (EC 6.2.1.12) 4CL2, 535 aa; fasta scores: opt: 1412 z-score: 1497.3 E(): 0; 44.2% identity in 532 aa overlap. Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and match to Prosite entry PS00455 Putative AMP-binding domain signature 4 3 6 20 [Reporter]SCO4702 (3P2)_PCR/5673 [Gene]SCO4702/5171 NC_003888 SCD31.27, rplC, 50S ribosomal protein L3, len: 214 aa; highly similar to SW:RL3_ECOLI (EMBL:V00344) Escherichia coli 50S ribosomal protein L3, RplC, 209 aa; fasta scores: opt: 683 z-score: 783.3 E(): 0; 51.9% identity in 206 aa overlap. Contains Pfam match to entry PF00297 Ribosomal_L3, Ribosomal protein L3 and match to Prosite entry PS00474 Ribosomal protein L3 signature 4 3 6 19 [Reporter]SCO7201 (4L2)_PCR/5672 [Gene]SCO7201/5170 NC_003888 SC1D2.04, possible transcriptional regulator, len: 158 aa. Similar to several e.g. Streptomyces coelicolor TR:Q9X8J3(EMBL:AL049841) putative transcriptional regulator, SCE9.21C (148 aa), fasta scores opt: 200 z-score: 250.8 E(): 1.7e-06 32.1% identity in 134 aa overlap. Contains a Pfam match to entry PF01047 MarR, MarR family and has a putative helix-turn-helix motif situated between residues 50..71 (+2.73 SD). 4 3 6 18 [Reporter]SCO6343 (5H2)_PCR/5671 [Gene]SCO6343/5169 NC_003888 SC3A7.11, questionable ORF, len: 42 aa 4 3 6 17 [Reporter]SCO7347 (6D2)_PCR/5670 [Gene]SCO7347/5168 NC_003888 SC9H11.01, unknown, partial CDS, len: >201 aa.,SC410.26, unknown, partial CDS, len: >55 aa. Overlaps and extends into CDS SC9H11.01 on the adjoining cosmid. 4 3 6 16 [Reporter]SCO4841 (6P22)_PCR/5669 [Gene]SCO4841/5167 NC_003888 SC5G8.09c, possible short-chain dehydrogenase, len: 256aa; similar to many proposed oxidoreductases eg. similar to many eg. SW:P43168 (YMP3_STRCO) hypothetical oxidoreductase from Streptomyces coelicolor (251 aa) fasta scores; opt: 1120, z-score: 1262.0, E(): 0, 73.6% identity in 250 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature. 4 3 6 15 [Reporter]SCO6963 (7L22)_PCR/5668 [Gene]SCO6963/5166 NC_003888 SC6F7.16c, possible lipoprotein, len: 281 aa. Weakly similar to Streptomyces coelicolor TR:Q54398 (EMBL:L42758) proteinase, SlpD or SCH5.02C (539 aa), fasta scores opt: 137 z-score: 151.5 E(): 0.54 29.5% identity in 295 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 4 3 6 14 [Reporter]SCO5938 (8H22)_PCR/5667 [Gene]SCO5938/5165 NC_003888 SC7H1.08c, unknown, len: 202 aa; some similarity in C-terminus to Mycobacterium tuberculosis hypothetical protein TR:P71839 (EMBL:Z80226) MTCY369.30C (129 aa), fasta scores; opt: 240 z-score: 383.0 E(): 3.8e-14, 41.2% identity in 102 aa overlap 4 3 6 13 [Reporter]SCO6058 (9D22)_PCR/5666 [Gene]SCO6058/5164 NC_003888 SC9B1.05, possible hydrolase, len: 265aa; similar to hypotheticals eg. TR:P71968 (EMBL:Z80225) from Mycobacterium tuberculosis (258 aa) fasta scores; opt: 536, z-score: 585.9, E(): 2.6e-25, (44.3% identity in 235 aa overlap). Also similar to several proposed ribofavin deaminases eg. SW:RIBG_BACAM ribofavin-specific deaminase from Bacillus subtilis (371 aa) fasta scores; opt: 157, z-score: 177.0, E(): 0.015, (28.3% identity in 180 aa overlap). 4 3 6 11 [Reporter]SCO4610 (11L18)_PCR/5664 [Gene]SCO4610/5163 NC_003888 SCD39.10, possible integral membrane protein, len: 138 aa; similar to TR:O53882 (EMBL:AL022004) Mycobacterium tuberculosis hypothetical 13.7 kDa protein MTV043.63c, 129 aa; fasta scores: opt: 509 z-score: 623.4 E(): 3e-27; 63.1% identity in 122 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 6 9 [Reporter]SCO0068 (13D18)_PCR/5662 [Gene]SCO0068/5162 NC_003888 SCJ4.49, possible hydrolase (putative secreted protein), len: 401aa; N-terminal region similar to that of TR:Q9Z4P9 (EMBL:AB024331) alpha-1,6-D-mannanase from Bacillus circulans (589 aa) fasta scores; opt: 218, z-score: 251.2, E(): 1.2e-06, (26.2% identity in 229 aa overlap) 4 3 6 8 [Reporter]SCO0422 (14P14)_PCR/5661 [Gene]SCO0422/5161 NC_003888 SCF51.21c, possible two-component sensor kinase, len: 397 aa; similar to TR:CAB51250 (EMBL:AL096872) Streptomyces coelicolor putative two-component sensor kinase, SC5F7.35c, 416 aa; fasta scores: opt: 841 z-score: 875.2 E(): 0; 43.5% identity in 393 aa overlap and to TR:Q53893 (EMBL:U51332) Streptomyces coelicolor AbsA1,571 aa; fasta scores: opt: 369 z-score: 385.9 E(): 4e-14; 30.5% identity in 394 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 6 7 [Reporter]SCO1593 (15L14)_PCR/5660 [Gene]SCO1593/5160 NC_003888 SCI35.15c, possible transcriptional regulator, len: 446 aa; similar to putative transcriptional regulators in S. griseus and S. colelicolor: SP15_STRGR 55.5 kD and 49.5 sporulation proteins (529 aa), fasta scores; opt: 399 z-score: 694.6 E(): 1.9e-31, 31.5% identity in 505 aa overlap and TR:Q53958 (EMBL:M80614) sporulation-associated protein genes (473 aa), fasta scores; opt: 405 z-score: 692.7 E(): 2.5e-31, 35.2% identity in 449 aa overlap. Contains PS00041 Bacterial regulatory proteins, araC family signature 4 4 23 22 [Reporter]SCO1754 (1L21)_PCR/8063 [Gene]SCO1754/7336 NC_003888 2SCI34.07c, unknown, len: 71 aa 4 4 23 21 [Reporter]SCO5822 (2H21)_PCR/8062 [Gene]SCO5822/7335 NC_003888 SC5B8.12, gyrB2, probable DNA gyrase subunit B, len: 707 aa; highly similar to many e.g. GYRB_STAAU P20832 staph ylococcus aureus. dna gyrase subunit B (EC 5.99.1.3) (643 a a), fasta scores; opt: 954 z-score: 2020.9 E(): 0, 45.2% id entity in 683 aa overlap. Contains PS00177 DNA topoisomeras e II signature and Pfam match to entry DNA_topoisoII PF0020 4, DNA topoisomerase II, score 609.86 4 4 23 20 [Reporter]SCO7382 (3D21)_PCR/8061 [Gene]SCO7382/7334 NC_003888 SC10G8.09c, unknown, len: 104 aa. Weakly similar to a protein of unknown function from Streptomyces coelicolor TR:Q9Z548(EMBL:AL035212) hypothetical 11.6 KD protein, SC9B2.11 (109 aa), fasta scores opt: 101 z-score: 134.7 E():4.5 35.1% identity in 74 aa overlap. 4 4 23 19 [Reporter]SCO7606 (4P17)_PCR/8060 [Gene]SCO7606/7333 NC_003888 SC2H2.04, possible amino acid binding protein, len: 373 aa; low similarity to TR:O68027 (EMBL:AF010496) Rhodobacter capsulatus potential Leu/Ile/Val-binding protein precursor, 400 aa; fasta scores: opt: 226 z-score: 253.4 E(): 1.4e-06; 25.2% identity in 322 aa overlap 4 4 23 18 [Reporter]SCO6698 (5L17)_PCR/8059 [Gene]SCO6698/7332 NC_003888 SC4C6.08c, pcaB, probable 3-carboxy-cis,cis-muconate cycloisomerase, len: 454 aa; similar to e.g. SW:PCAB_BRAJA (EMBL:Y10223), pcaB, 3-carboxy-cis,cis-muconate cycloisomerase (451 aa), fasta scores; opt: 996 z-score: 1027.1 E(): 0, 43.3% identity in 448 aa overlap. Contains Pfam match to entry PF00206 lyase_1, Lyase and PS00163 Fumarate lyases signature 4 3 6 6 [Reporter]SCO3711 (16H14)_PCR/5659 [Gene]SCO3711/5159 NC_003888 SCH35.13c, possible small membrane protein, len: 103 aa; contains possible membrane spanning hydrophobic regions. 4 4 23 17 [Reporter]SCO7550 (6H17)_PCR/8058 [Gene]SCO7550/7331 NC_003888 SC5F1.04c, possible secreted hydrolase, len: 284 aa; similar to SW:GLPQ_BACSU (EMBL:Z26522) Bacillus subtilis glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46) GlpQ, 293 aa; fasta scores: opt: 354 z-score: 431.1 E(): 1.7e-16; 27.8% identity in 284 aa overlap. Contains possible N-terminal region signal peptide sequence 4 3 6 5 [Reporter]SCO3213 (17D14)_PCR/5658 [Gene]SCO3213/5158 NC_003888 SCE8.06c, trpG, anthranilate synthase component II, len: 200aa; similar to many eg. TR:G4185545 (EMBL:AF108766) TrpG, anthranilate synthase component II, from Rhodobacter sphaeroides (195 aa) fasta scores; opt: 728, z-score: 825.0, E(): 0, (60.2% identity in 186 aa overlap). Contains matches to Pfam entry PF00117 GATase, Glutamine amidotransferases class-I, score 332.60, E-value 4.3e-96 and Prosite PS00442 Glutamine amidotransferases class-I active site. 4 4 23 16 [Reporter]SCO2663 (7D17)_PCR/8057 [Gene]SCO2663/7330 NC_003888 SC6D10.06, possible secreted protein, len: 429 aa; similar to TR:P96258 (EMBL:Z84724) Mycobacterium tuberculosis hypothetical 47.1 kD protein MTCY22G10.08c, 439 aa; fasta scores: opt: 430 z-score: 482.8 E(): 1.8e-19; 30.9% identity in 427 aa overlap and to TR:Q9ZBL6 (EMBL:AL035159) Mycobacterium leprae putative membrane protein MLCB1450.21c, 403 aa; fasta scores: opt: 412 z-score: 463.3 E(): 2.1e-18; 29.6% identity in 423 aa overlap. Contains possible N-terminal region signal peptide sequence 4 3 6 4 [Reporter]SCO1635 (18P10)_PCR/5657 [Gene]SCO1635/5157 NC_003888 SCI41.18c, unknown, len: 103 aa 4 3 6 3 [Reporter]SCO1318 (19L10)_PCR/5656 [Gene]SCO1318/5156 NC_003888 SCBAC36F5.29, putative membrane protein, len: 327 aa; C-terminal region similar to TR:Q9K414 (EMBL:AL359949) Streptomyces coelicolor hypothetical 18.8 kDa protein (fragment) 2SCG61.01, 174 aa; fasta scores: opt: 528 Z-score: 469.6 bits: 94.3 E(): 1.5e-18; 46.429% identity in 168 aa overlap. Contains possible hydrophobic membrane spanning region in N-terminal domain 4 3 6 2 [Reporter]SCO1310 (20D10)_PCR/5655 [Gene]SCO1310/5155 NC_003888 SCBAC36F5.21, possible cation efflux system protein, len: 363 aa; similar to TR:AAK46363 (EMBL:AE007059) Mycobacterium tuberculosis CDC1551 MT2084, 332 aa; fasta scores: opt: 1199 Z-score: 1252.9 bits: 240.3 E(): 3.6e-62; 59.627% identity in 322 aa overlap. Contains Pfam match to entry PF01545 Cation_efflux, Cation efflux family and possible hydrophobic membrane spanning regions 4 3 5 22 [Reporter]SCO5388 (1D6)_PCR/5653 [Gene]SCO5388/5154 NC_003888 2SC6G5.32c, conserved hypothetical protein, len: 251 aa; similar to many eg. SW:Q10634 (Y036_MYCTU) hypothetical protein from Mycobacterium tuberculosis (226 aa) fasta scores; opt: 1161, z-score: 1353.2, E(): 0, 76.1% identity in 226 aa overlap. Contains Pfam match to entry PF01939 DUF91, Protein of unknown function. 4 3 5 21 [Reporter]SCO1244 (2P2)_PCR/5652 [Gene]SCO1244/5153 NC_003888 2SCG1.19, bioB, biotin synthase, len: 407 aa; similar to SW:BIOB_BACSU (EMBL:U51868) Bacillus subtilis biotin synthase (EC 2.8.1.6) BioB, 335 aa; fasta scores: opt: 1067 z-score: 1082.3 E(): 0; 49.1% identity in 328 aa overlap. Contains Pfam match to entry PF01792 Biotin_synth, Biotin synthase 4 3 5 20 [Reporter]SCO5812 (3L2)_PCR/5651 [Gene]SCO5812/5152 NC_003888 SC5B8.02, probable ribonuclease HII, len: 233 aa; si milar to many e.g. RNH2_ECOLI P10442 ribonuclease hii (EC 3 .1.26.4) (213 aa), fasta scores; opt: 444 z-score: 689.5 E( ): 3.2e-31, 42.5% identity in 179 aa overlap 4 3 5 19 [Reporter]SCO6899 (4H2)_PCR/5650 [Gene]SCO6899/5151 NC_003888 SC1B2.05, possible membrane protein, len: 383 aa. Similar to several putative membrane transport proteins e.g. Rhizobium sp. (strain NGR234) SW:Y4WD_RHISN(EMBL:AE000103) hypothetical transport protein (377 aa), fasta scores opt: 586 z-score: 584.6 E(): 4.2e-25 32.9% identity in 380 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 3 5 18 [Reporter]SCO6339 (5D2)_PCR/5649 [Gene]SCO6339/5150 NC_003888 SC3A7.07, probable 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase, len: 449 aa; Similar in N- and C- termini to Rhodococcus opacus TR:G2935027 (EMBL:EMBL; AF003947) 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase (400 aa), fasta scores; opt: 551 z-score: 916.0 E(): 0, 35.2% identity in 435 aa overlap. N-terminus (to 250aa) is similar to e.g. Acinetobacter calcoaceticus TR:Q59093 (EMBL:L05770) beta-ketoadipate enol-lactone hydrolase (266 aa), fasta scores; opt: 445 z-score: 470.5 E(): 5.9e-19, 30.3% identity in 244 aa overlap. C-terminus (from 330 aa) is similar to e.g. DC4C_ACICA 4-carboxymuconolactone decarboxylase (134 aa), fasta scores; opt: 409 z-score: 435.1 E(): 5.5e-17, 47.2% identity in 123 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha /beta hydrolase fold, score 62.50, E-value 8.9e-15 4 4 23 15 [Reporter]SCO1989 (8P13)_PCR/8056 [Gene]SCO1989/7329 NC_003888 SC7H2.03c, probable aminopeptidase, len: 323 aa; similar to many proline aminopeptidases e.g. SW:PIP_XANCI proline iminopeptidase from Xanthomonas campestris pv. citri (313 aa) fasta scores; opt: 848, z-score: 979.3, E(): 0, (44.4% identity in 320 aa overlap). Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 4 4 23 14 [Reporter]SCO5840 (9L13)_PCR/8055 [Gene]SCO5840/7328 NC_003888 SC9B10.07, probable transcriptional regulator, len: 231 aa; similar to many eg. GNTR_BACSU P10585 gluconate operon transcriptional repressor (243 aa), fasta scores; opt: 199 z-score: 264.8 E(): 1.4e-07, 26.2% identity in 195 aa overlap. Contains PS00043 Bacterial regulatory proteins, gntR family signature. Contains probable helix-turn-helix motif at aa positions 29-49 (Score 1133, +3.05 SD) 4 4 23 13 [Reporter]SCO1929 (10H13)_PCR/8054 [Gene]SCO1929/7327 NC_003888 SCC22.11, possible integral membrane transport protein, len: 269aa; function predicted as it contains possible membrane spanning hydrophobic regions and is located alongside a possible ABC transport ATP-binding protein (SCC22.10). Similar to egs. TR:O86349 (EMBL:AL021184) hypothetical protein from Mycobacterium tuberculosis (261 aa) fasta scores; opt: 627, z-score: 670.8, E(): 4.9e-30, (43.6% identity in 264 aa overlap) and TR:AAD20380 (EMBL:AF125999) daunorubicin resistance protei n C from Mycobacterium avium (263 aa) fasta scores; opt: 266, z-score: 291.2, E(): 7e-09, (29.6% identity in 247 aa overlap). 4 4 23 12 [Reporter]SCO4553 (11D13)_PCR/8053 [Gene]SCO4553/7326 NC_003888 SCD16A.30c, putative membrane protein, len: 139 aa. Contains possible hydrophobic membrane spanning regions 4 4 23 11 [Reporter]SCO4146 (12P9)_PCR/8052 [Gene]SCO4146/7325 NC_003888 SCD84.13, probable ECF subfamily sigma factor, len: 180 aa; similar to TR:Q9WWP4 (EMBL:AF121850) Synechococcus sp. SigH, 199 aa; fasta scores: opt: 273 z-score: 333.6 E(): 4e-11; 37.4% identity in 163 aa overlap and to TR:O05767 (EMBL:U87307) Mycobacterium smegmatis extracytoplasmic function alternative sigma factor, SigE, 204 aa; fasta scores: opt: 261 z-score: 319.3 E(): 2.5e-10; 33.5% identity in 188 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 4 4 23 10 [Reporter]SCO3106 (13L9)_PCR/8051 [Gene]SCO3106/7324 NC_003888 SCE41.15, possible lipoprotein, len: 299 aa; similar to TR:CAB76294 (EMBL:AL158057) Streptomyces coelicolor putative lipoprotein SC10G8.21c, 249 aa; fasta scores: opt: 407 z-score: 384.9 E(): 6.2e-14; 31.1% identity in 241 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 4 23 9 [Reporter]SCO1700 (14H9)_PCR/8050 [Gene]SCO1700/7323 NC_003888 SCI30A.21c, putative membrane protein, len: 104 aa. Contains possible hydrophobic membrane spanning regions 4 4 23 8 [Reporter]SCO3816 (15D9)_PCR/8049 [Gene]SCO3816/7322 NC_003888 SCGD3.17c, bkdB1, probable branched-chain alpha keto acid dehydrogenase E1 beta subunit, len: 326 aa; similar to E1 beta subunits from pyruvate dehydrogenase complexes and branched-chain alpha keto acid dehydrogenases e.g. TR:Q53593 (EMBL:U1716), BkdB, Streptomyces avermitilis E1-beta branched-chain alpha keto acid dehydrogenase (334 aa), fasta scores; opt: 1188 z-score: 1395.2 E(): 0, 53.8% identity in 325 aa overlap and SW:ODPB_BACST (EMBL:X53560), PdhB, Bacillus stearothermophilus pyruvate dehydrogenase complex E1 beta subunit (324 aa) (48.0% identity in 323 aa overlap). Highly similar to SCGD3.31c, probable dihydrolipoamide acyltransferase component E2 (334 aa) (55.2% identity in 324 aa overlap), in a duplication of this region upstream 4 3 5 16 [Reporter]SCO4836 (6L22)_PCR/5647 [Gene]SCO4836/5149 NC_003888 SC5G8.04, possible gntR-family regulator, len: 462 aa; similar to many proposed regulators eg. TR:AAF01064 (EMBL:AF189258) transcriptional regulator (fragment) from Streptomyces venezuelae (507 aa) fasta scores; opt: 1010, z-score: 1115.6, E(): 0, 45.2% identity in 389 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. 4 4 23 7 [Reporter]SCO0616 (16P5)_PCR/8048 [Gene]SCO0616/7321 NC_003888 SCF55.40c, hypothetical protein, len: 791 aa; similar to various hypothetical proteins, e.g. SW:YK06_MYCTU (EMBL:Z74025) Mycobacterium tuberculosis hypothetical 145.8 KD protein RV2006, 1327 aa; fasta scores: opt: 2445 z-score: 2870.6 E(): 0; 50.0% identity in 790 aa overlap 4 3 5 15 [Reporter]SCO7435 (7H22)_PCR/5646 [Gene]SCO7435/5148 NC_003888 SC6D11.31c, possible transmembrane transport protein, len: 422 aa. Similar to many transport proteins e.g. Acinetobacter sp. TR:O30513(EMBL:AF009224) benzoate transport protein, BenK (466 aa), fasta scores opt: 423 z-score: 455.2 E(): 6.2e-18 31.1% identity in 421 aa overlap. Contains a Prosite hit to PS00216 Sugar transport proteins signature 1 and a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter. Contains multiple possible membrane spanning hydrophobic domains. 4 4 23 6 [Reporter]SCO4999 (17L5)_PCR/8047 [Gene]SCO4999/7320 NC_003888 SCK15.01, hypothetical protein, len: 162 aa; similar to TR:CAB7697 (EMBL:AL159178) Streptomyces coelicolor hypothetical 16.8 kD protein SCH22A.02c, 160 aa; fasta scores: opt: 133 z-score: 165.9 E(): 0.09; 28.9% identity in 142 aa overlap 4 3 5 14 [Reporter]SCO5933 (8D22)_PCR/5645 [Gene]SCO5933/5147 NC_003888 SC7H1.03c, possible membrane protein, len: 391 aa; C-terminal part similar to TR:Q9L0E7 (EMBL:AL161803) Streptomyces coelicolor hypothetical protein SCD31.20, 181 aa; fasta scores: opt: 592 Z-score: 665.9 E(): 1.8e-29; 53.514% identity in 185 aa overlap. Contains possible hydrophobic membrane spanning regions. Contains PS00213 Lipocalin signature, though this may be spurious 4 3 5 13 [Reporter]SCO6443 (9P18)_PCR/5644 [Gene]SCO6443/5146 NC_003888 SC9B5.10, conserved hypothetical protein, len: 303aa; similar to many hypothetical proteins e.g. TR:O86359 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (314 aa) fasta scores; opt: 464, z-score: 847.2, E(): 0, (36.7% identity in 313 aa overlap). Contains possible N-terminal signal sequence 4 3 5 12 [Reporter]SCO1960 (10L18)_PCR/5643 [Gene]SCO1960/5145 NC_003888 SCC54.20, possible hydrolase, len: 218aa; similar to many hypothetical proteins eg. TR:O06154 (EMBL:Z95554) hypothetical protein from Mycobacterium tuberculosis fasta scores; opt: 732. z-score: 872.3. E(): 0, (52.3% identity in 220 aa overlap). Also has a region of similarity to SW:GLO2_RHOBL probable hydrocylglutathione hydrolase from Rhodopseudomonas blastica (255 aa) fasta scores; opt: 275, z-score: 333.2, E(): 3e-11, (33.7% identity in 193 aa overlap). 4 3 5 11 [Reporter]SCO4583 (11H18)_PCR/5642 [Gene]SCO4583/5144 NC_003888 SCD20.01, putative polyprenyl diphosphate synthase, len: 336 aa; similar to SW:HEP2_BACSU (EMBL:M80245) Bacillus subtilis heptaprenyl diphosphate synthase component II (EC 2.5.1.30) HepT, 348 aa; fasta scores: opt: 586 z-score: 674.6 E(): 4.2e-30; 33.0% identity in 309 aa overlap. Contains Pfam match to entry PF00348 polyprenyl_synt, Polyprenyl synthetases and match to Prosite entry PS00444 Polyprenyl synthetases signature 2 4 3 5 10 [Reporter]SCO4286 (12D18)_PCR/5641 [Gene]SCO4286/5143 NC_003888 SCD95A.19, possible solute-binding protein (putative secreted protein), len: 421 aa; similar to TR:P72397 (EMBL:Y07706) Streptomyces coelicolor putative maltose-binding protein MalE, 423 aa; fasta scores: opt: 348 z-score: 387.3 E(): 4.1e-14; 25.9% identity in 433 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and PS01037 Bacterial extracellular solute-binding proteins, family 1 signature. Contains also possible N-terminal signal peptide sequence 4 3 5 9 [Reporter]SCO3498 (13P14)_PCR/5640 [Gene]SCO3498/5142 NC_003888 SCE65.34c, possible secreted protein, len: 358 aa; similar to TR:P94576 (EMBL:Z82987) Bacillus subtilis hypothetical 51.5 kD protein YwoF, 468 aa; fasta scores: opt: 171 z-score: 188.8 E(): 0.0038; 24.1% identity in 403 aa overlap. Contains possible N-terminal region signal peptide sequence and Leucine TTA codon, possible target for bldA regulation 4 3 5 8 [Reporter]SCO0224 (14L14)_PCR/5639 [Gene]SCO0224/5141 NC_003888 SCJ9A.03c, unknown, len: 345aa; similar to TR:CAB45045 (EMBL:AL078635) hypothetical protein from Amycolatopsis orientalis (353 aa) fasta scores; opt: 1018, z-score: 1095.3, E(): 0, (47.3% identity in 355 aa overlap). 4 3 5 7 [Reporter]SCO0929 (15H14)_PCR/5638 [Gene]SCO0929/5140 NC_003888 SCM10.17c, hypothetical protein, len: 270 aa; similar to various S. coelicolor hypothetical proteins, e.g. TR:O86540 (EMBL:AL031350) Streptomyces coelicolor hypothetical 32.8 kD protein SC1F2.03c, 300 aa; fasta scores: opt: 713 z-score: 830.1 E(): 0; 45.0% identity in 262 aa overlap 4 4 23 5 [Reporter]SCO1549 (18H5)_PCR/8046 [Gene]SCO1549/7319 NC_003888 SCL11.05c, hypothetical protein, len: 312 aa; similar to TR:O33744 (EMBL:Y10293) Streptomyces sp. hypothetical 33.8 kD protein 312 aa; fasta scores: opt: 206 z-score: 221.9 E(): 6.1e-05; 29.7% identity in 273 aa overlap 4 4 23 4 [Reporter]SCO7466 (19D5)_PCR/8045 [Gene]SCO7466/7318 NC_003888 SCBAC14E8.06, cvnD13, possible ATP/GTP-binding protein, len: 176 aa; similar to TR:Q9XAE1 (EMBL:AL079356) Streptomyces coelicolor putative ATP/GTP-binding protein SC6G9.41c, 191 aa; fasta scores: opt: 615 Z-score: 755.2 bits: 146.4 E(): 1.7e-34; 55.172% identity in 174 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 4 23 3 [Reporter]SCO6290 (20P1)_PCR/8044 [Gene]SCO6290/7317 NC_003888 SCBAC8D1.03, hypothetical protein, len: 84 aa; highly similar to N-terminal part of SW:SOXA_RHIME (EMBL:AF055582) Rhizobium meliloti sarcosine oxidase alpha subunit (EC 1.5.3.1) SoxA, 987 aa; fasta scores: opt: 132 Z-score: 169.8 bits: 39.3 E(): 0.072l; 42.373% identity in 59 aa overlap. Contains Pfam match to entry PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains 4 4 22 22 [Reporter]SCO1142 (1H21)_PCR/8042 [Gene]SCO1142/7316 NC_003888 2SCG38.35, possible oxidoreductase, len: 359 aa; similar to TR:Q9X8X9 (EMBL:AL078610) Streptomyces coelicolor putative oxidoreductase SCH35.27, 406 aa; fasta scores: opt: 670 z-score: 758.0 E(): 0; 50.6% identity in 235 aa overlap. Contains Pfam match to entry PF00743 FMO-like, Flavin-binding monooxygenase-like 4 4 22 21 [Reporter]SCO6507 (2D21)_PCR/8041 [Gene]SCO6507/7315 NC_003888 SC1E6.16, gvpS, probable gas vesicle synthesis protein, len: 60 aa; similar to parts of several gas vesicle proteins e.g. Bacillus megaterium TR:O68671 (EMBL:AF053765) gas vesicle protein GVPS (95 aa), fasta scores; opt: 163 z-score: 303.6 E(): 1.2e-09, 49.1% identity in 57 aa overlap, and TR:O68669 (EMBL:AF053765) gas vesicle protein GVPJ (100 aa), fasta scores; opt: 130 z-score: 256.8 E(): 5.1e-07, 45.2% identity in 42 aa overlap. Contains Pfam match to entry PF00741 Gas_vesicle, Gas vesicle protein, score 31.70, E-value 1.7e-05 4 4 22 20 [Reporter]SCO6418 (3P17)_PCR/8040 [Gene]SCO6418/7314 NC_003888 SC1A6.07c, unknown, len: 813 4 4 22 19 [Reporter]SCO7233 (4L17)_PCR/8039 [Gene]SCO7233/7313 NC_003888 SC2H12.32, possible secreted protein, len: 934 aa. Note the product of this CDS is rich in the amino acid proline. Contains a possible N-terminal signal sequence. Contains a TTA /leucine codon, possible target for bldA regulation. 4 4 22 18 [Reporter]SCO2410 (5H17)_PCR/8038 [Gene]SCO2410/7312 NC_003888 SC4A7.38c, possible integral membrane protein, len: 253 aa; similar to various hypothetical proteins, e.g. TR:O07422 (EMBL:Z97050) Mycobacterium tuberculosis hypothetical 25.9 kD protein MTCI28.18, 244 aa; fasta scores: opt: 183 z-score: 204.4 E(): 0.00053; 31.0% identity in 171 aa overlap and to S. coelicolor SC4A7.40c, 219 aa; fasta scores: opt: 323 z-score: 280.2 E(): 3.2e-10; 40.5% identity in 200 aa overlap 4 3 5 6 [Reporter]SCO3356 (16D14)_PCR/5637 [Gene]SCO3356/5139 NC_003888 SCE94.07, sigE, ECF sigma factor, len: 177 aa; previously sequenced therefore identical to TR:E1358525 (EMBL:AJ131213) SigE, ECF sigma factor from Streptomyces coelicolor (177 aa) fasta scores; opt: 1140, z-score: 1336.4, E(): 0, (100.0% identity in 177 aa overlap). Member of ECF sigma factors. Contains Prosite match to PS01063 Sigma-70 factors ECF subfamily signature. Also contains a possible helix-turn-helix region at 127-148aa (+3.34 SD). 4 4 22 17 [Reporter]SCO7791 (6D17)_PCR/8037 [Gene]SCO7791/7311 NC_003888 SC5E9.38c, possible secreted oxidoreductase, len: 401 aa; similar to many e.g. TR:O53772 (EMBL:AL021942) putative oxidoreductase from Mycobacterium tuberculosis (388 aa) fasta scores; opt: 524, z-score: 584.4, E(): 4.4e-25, 32.4% identity in 373 aa overlap. Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase. 4 3 5 5 [Reporter]SCO0643 (17P10)_PCR/5636 [Gene]SCO0643/5138 NC_003888 SCF91.03c, possible secreted cellulose-binding protein, len: 228 aa. Similar to several including: Streptomyces coelicolor TR:CAB50949 (EMBL:AL096849) putative secreted cellulose-binding protein SCI11.23 (356 aa), fasta scores opt: 349 z-score: 417.9 E(): 6.7e-16 32.2% identity in 227 aa overlap. Also weakly similar to chitin-binding proteins e.g. Streptomyces olivaceoviridis TR:Q54501 (EMBL:X78535) chitin binding protein precursor (201 aa), fasta scores opt: 203 z-score: 249.8 E(): 1.5e-06 29.3% identity in 222 aa overlap. Contains a possible N-terminal signal sequence. 4 4 22 16 [Reporter]SCO1419 (7P13)_PCR/8036 [Gene]SCO1419/7310 NC_003888 SC6D7.20c, putative phosphodiesterase, len: 255 aa. Similar to several including Escherichia coli SW:UGPQ_ECOLI(EMBL:X14437) glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46) (247 aa), fasta scores opt: 215 z-score: 267.5 E(): 1.6e-07 27.0% identity in 252 aa overlap and Mycobacterium tuberculosis TR:O07244(EMBL:Z96800) hypothetical 28.3 KD protein (256 aa), fasta scores opt: 632 z-score: 771.7 E(): 0 44.2% identity in 251 aa overlap. 4 3 5 4 [Reporter]SCO1695 (18L10)_PCR/5635 [Gene]SCO1695/5137 NC_003888 SCI30A.16, unknown, len: 200aa; 4 3 5 3 [Reporter]SCP1.196 (19H10)_PCR/5634 [Gene]SCP1.196/5136 NC_003888 SCP1.196, possible DNA primase/helicase, len: 506aa; previously sequenced and annotated as TR:Q9L8S3 (EMBL:AF195093), note discrepency: SCP1.196 translational start site is 239 bases upstream of that designated for TR:Q9L8S3. Similar to others eg. TR:Q9ZX10 (EMBL:AF068845) GP70 from Mycobacteriophage TM4 (867 aa) fasta scores; opt: 1104, z-score: 1313.5, E(): 0, 42.6% identity in 470 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 3 5 2 [Reporter]SCO1854 (20P6)_PCR/5633 [Gene]SCO1854/5135 NC_003888 SCI39.01c, possible integral membrane protein (fragment), len: >174 aa. Contains possible hydrophobic membrane spanning regions,SCI8.39, possible permease, partial CDS, len: > 104 aa. Weakly similar to Escherichia coli SW:BTUC_ECOLI (EMBL: M14031) vitamin B12 transport system permease protein (326 aa), fasta scores opt: 101 z-score: 140.5 E():1.9 32.5% identity in 77 aa overlap. Contains possible membrane spanning hydrophobic domains. 4 3 4 22 [Reporter]SCO4548 (1P2)_PCR/5631 [Gene]SCO4548/5134 NC_003888 SCD16A.35c, partial CDS, unknown, len: >726 aa,2SCD4.19, possible integral membrane protein (fragment), len: >309 aa. Contains possible hydrophobic membrane spanning regions 4 3 4 21 [Reporter]SCO5258 (2L2)_PCR/5630 [Gene]SCO5258/5133 NC_003888 2SC7G11.20c, atrC, ATP-binding protein, len: 253 aa; previously sequenced as TR:Q9RGW6 (EMBL:AF104994) Streptomyces coelicolor A3(2) ATP-binding protein AtrC, 239 aa. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 4 3 4 20 [Reporter]SCO5033 (3H2)_PCR/5629 [Gene]SCO5033/5132 NC_003888 SCK7.06, oxyR, hydrogen peroxide sensing regulator, len: 312 aa; identical to previously sequenced TR:Q9RN71 (EMBL:AF186371) Streptomyces coelicolor A3(2) hydrogen peroxide sensing regulator OxyR, 313 aa. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry S00044 Bacterial regulatory proteins, lysR family signature. Also contains possible helix-turn-helix motif at residues 23..44 (+3.89 SD) 4 3 4 19 [Reporter]SCO6165 (4D2)_PCR/5628 [Gene]SCO6165/5131 NC_003888 SC6C5.01, partial CDS, unknown, len: 121aa; similar to TR:O83134 (EMBL:AE001194) putative dnaK suppressor from Treponema pallidum (120 aa) fasta scores; opt: 162, z-score: 252.5, E(): 8.9e-07, (40.7% identity in 59 aa overlap).,SC1A9.29c, partial CDS, unknown, len: 38aa 4 3 4 18 [Reporter]SCO2028 (4P22)_PCR/5627 [Gene]SCO2028/5130 NC_003888 SC3A3.06, possible membrane protein, len: 509 aa; unknown function, probable Ser- and Pro-rich CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains a compound repeat region at the C-terminus consisting of 22 degenerate repeats (19x RSPP(A/D)PD and 3x AHAPAPD). Shows weak similarity to the neighbouring CDS SC3A3.07, SC3A3.08 and SC3A3.09 e.g. SC3A3.07 hypothetical protein (425 aa) (32.1% identity in 383 aa overlap). Contains a hydrophobic, possible membrane-spanning region 4 4 22 15 [Reporter]SCO5963 (8L13)_PCR/8035 [Gene]SCO5963/7309 NC_003888 SC7H1.33c, possible membrane protein, len: 191; Contains PS00044 Bacterial regulatory proteins, lysR family signature, though this may be spurious 4 4 22 14 [Reporter]SCO5418 (9H13)_PCR/8034 [Gene]SCO5418/7308 NC_003888 SC8F4.22c, possible transcriptional regulator, len: 234aa; similar to many eg. TR:Q54288 (EMBL:X86780) regulator of antibiotic transport complexes from Streptomyces hygroscopicus (204 aa) fasta scores; opt: 284, z-score: 301.4, E(): 2.2e-09, 31.3% identity in 211 aa overlap. Contains helix-turn-helix motif Score 1083 (+2.88 SD) in ID at residue 38. 4 4 22 13 [Reporter]SCO1925 (10D13)_PCR/8033 [Gene]SCO1925/7307 NC_003888 SCC22.07c, conserved hypothetical protein, len: 473aa; similar to many eg. TR:O32162 (EMBL:Z99120) hypothetical protein from Bacillus subtilis (465 aa) fasta scores; opt: 2087, z-score: 2451.5, E(): 0, (64.6% identity in 463 aa overlap). Also similar to the N-terminal region and the C-terminal region of TR:O53152 (EMBL:AL021184) hypothetical splice protein from Mycobacterium tuberculosis (846 aa) fasta scores; opt: 1367, z-score: 1602.5, E(): 0, (47.5% identity in 832 aa overlap). Contains Pfam match to entry PF01458 UPF0051, Uncharacterized protein family (UPF0051). 4 4 22 12 [Reporter]SCO4327 (11P9)_PCR/8032 [Gene]SCO4327/7306 NC_003888 SCD12A.10c, hypothetical protein, len: 209 aa; similar to TR:AAF11232 (EMBL:AE002009) Deinococcus radiodurans hypothetical 19.1 kD protein DR1664, 193 aa; fasta scores: opt: 444 z-score: 477.4 E(): 4e-19; 47.8% identity in 182 aa overlap 4 4 22 11 [Reporter]SCO4136 (12L9)_PCR/8031 [Gene]SCO4136/7305 NC_003888 SCD84.03c, hypothetical protein, len: 133 aa; similar to TR:O06070 (EMBL:Z95398) Mycobacterium leprae hypothetical 15.2 kD protein MLCL622.07, 135 aa; fasta scores: opt: 374 z-score: 470.4 E(): 9.6e-19; 55.9% identity in 102 aa overlap 4 4 22 10 [Reporter]SCO3245 (13H9)_PCR/8030 [Gene]SCO3245/7304 NC_003888 SCE29.14c, possible salicylate hydroxylase (putative secreted protein), len: 420 aa; similar to many e.g. TR:P96555 (EMBL:AB000564) salicylate hydroxylase from Sphingomonas sp. AJ1 (395 aa) fasta scores; opt: 830, z-score: 827.2, E(): 0, (41.9% identity in 320 aa overlap) and TR:G4104775 (EMBL:AF039534) salicylate hydroxylase from Pseudomonas stutzeri strain AN10 (389 aa) fasta scores; opt: 707, z-score: 706.0, E(): 5.1e-32, (34.7% identity in 401 aa overlap). Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase, score 91.00, E-value 2.4e-23. Contains possible N-terminal region signal peptide sequence 4 4 22 9 [Reporter]SCO2886 (14D9)_PCR/8029 [Gene]SCO2886/7303 NC_003888 SCE6.23c, possible hydrolase, len: 294 aa. Similar to several including: Mycobacterium sp. TR:Q9ZER0 (EMBL:AJ012627) haloalkane dehalogenase, DhaAF (307 aa), fasta scores opt: 347 z-score: 425.8 E(): 2.7e-16 27.4% identity in 292 aa overlap and Deinococcus radiodurans TR:AAF12090 (EMBL:AE002084) epoxide hydrolase-related protein (278 aa), fasta scores opt: 402 z-score: 493.2 E(): 4.8e-2032.4% identity in 284 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 4 4 22 8 [Reporter]SCO1791 (15P5)_PCR/8028 [Gene]SCO1791/7302 NC_003888 SCI51.31c, hypothetical protein, len: 419 aa; unknown function, improbable CDS containing Thr- and Val-rich repeats and having a Ser- and Arg-rich C-terminus. Due to the unusual base composition in this region this assignment should be treated with caution 4 3 4 17 [Reporter]SCO7059 (5L22)_PCR/5626 [Gene]SCO7059/5129 NC_003888 SC4G1.25c, possible oxidoreductase, len: 233 aa; similar to TR:Q51492 (EMBL:D84146) Pseudomonas aeruginosa reductase PahA, 328 aa; fasta scores: opt: 297 z-score: 346.1 E(): 8.3e-12; 32.0% identity in 203 aa overlap 4 4 22 7 [Reporter]SCO3309 (16L5)_PCR/8027 [Gene]SCO3309/7301 NC_003888 SCE68.07c, hypothetical protein, len: 125 aa; unknown function, probable CDS suggested by GC frame analysis and amino acid composition 4 3 4 16 [Reporter]SCO6720 (6H22)_PCR/5625 [Gene]SCO6720/5128 NC_003888 SC5F2A.03c, probable ABC transporter, len: 544aa; similar to many involved in antibiotic resistance egs. TR:Q54381 (EMBL:X79146) from the lincomycin-production gene cluster of Streptomyces lincolnensis 78-11 (578 aa) fasta scores; opt: 1624, z-score: 1696.3, E(): 0, (50.3% identity in 541 aa overlap). Contains two Pfam matches to entry PF00005 ABC_tran, ABC transporter, two Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop) and Prosite match to PS00211 ABC transporters family signature. Also contains a possible coiled-coil region around 250aa. 4 4 22 6 [Reporter]SCO0587 (17H5)_PCR/8026 [Gene]SCO0587/7300 NC_003888 SCF55.11c, cvnB11, hypothetical protein, len: 152 aa; similar to various hypothetical proteins, e.g. TR:O86521 (EMBL:AL031124) Streptomyces coelicolor hypothetical 14.7 KD protein SC1C2.20c, 140 aa; fasta scores: opt: 337 z-score: 422.9 E(): 3.5e-16; 41.3% identity in 138 aa overlap, to TR:CAB41561 (EMBL:AL049727) Streptomyces coelicolor hypothetical 14.4 KD protein SC9B1.15c, 139 aa; fasta scores: opt: 314 z-score: 395.1 E(): 1.2e-14; 41.5% identity in 142 aa overlap and to TR:Q9L138 (EMBL:AL158061c) Streptomyces coelicolor hypothetical protein SC6D11.17c, 135 aa; fasta scores: opt: 634 Z-score: 769.5 E(): 3.1e-35; 71.875% identity in 128 aa overlap 4 3 4 15 [Reporter]SCO7429 (7D22)_PCR/5624 [Gene]SCO7429/5127 NC_003888 SC6D11.25c, unknown, len: 116 aa. 4 3 4 14 [Reporter]SCO7596 (8P18)_PCR/5623 [Gene]SCO7596/5126 NC_003888 SC7H9.08c, probable integral membrane transport protein, len: 523 aa; similar to SW:SGLT_VIBPA (EMBL:D78137) Vibrio parahaemolyticus sodium/glucose co-transporter (Na(+)/glucose symporter) SglT, 543 aa; fasta scores: opt: 445 z-score: 513.1 E(): 4.8e-21; 24.4% identity in 471 aa overlap. Contains Pfam match to entry PF00474 SSF, Sodium:solute symporter family. Also contains possible hydrophobic membrane spanning regions 4 3 4 13 [Reporter]SCO6439 (9L18)_PCR/5622 [Gene]SCO6439/5125 NC_003888 SC9B5.06c, possible DNA-binding protein, len: 162 aa; similar in parts to many eukaryotic Y-box binding proteins (cold shock proteins) eg. SW:YB1_CHICK Y-box binding protein from Gallus gallus (chicken) (321 aa) fasta scores; opt: 165, z-score: 278.2, E(): 3.3e-08, (34.9% identity in 106 aa overlap) and TR:O53883 (EMBL:AL022004) proposed cold shock protein B from Mycobacterium tuberculosis (135 aa) fasta scores; opt: 174, z-score: 248.0, E(): 1.6e-06, (34.4% identity in 96 aa overlap). Contains Pfam match to entry PF00313 CSD, 'Cold-shock' DNA-binding domain. 4 3 4 12 [Reporter]SCO2461 (10H18)_PCR/5621 [Gene]SCO2461/5124 NC_003888 SCC24.32, possible secreted protein, len: 108 aa. Contains possible N-terminal region signal peptide sequence 4 3 4 10 [Reporter]SCO3292 (12P14)_PCR/5619 [Gene]SCO3292/5123 NC_003888 SCE15.09c, unknown, len: 169aa; 4 3 4 9 [Reporter]SCO0491 (13L14)_PCR/5618 [Gene]SCO0491/5122 NC_003888 SCF34.10c, probable ABC-transporter transmembrane protein, len: 540 aa; similar to transporters e.g. SW:PRTD_ERWCH (EMBL:M60395), PrtD, Erwinia chrysanthemi proteases secretion ATP-binding protein (575 aa), fasta scores; opt: 433 z-score: 468.5 E(): 9.7e-19, 29.1% identity in 413 aa overlap. Similar to TR:O87312 (EMBL:AF027770), FxtA, Mycobacterium smegmatis hypothetical protein at exochelin locus (574 aa) (39.7% identity in 524 aa overlap). Similar to others from S.coelicolor e.g. to the N-terminal half of TR:Q9ZBY5 (EMBL:AL034355), CydCD, Streptomyces coelicolor probable ABC transporter (1172 aa) (29.6% identity in 550 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and PS00211 ABC transporters family signature 4 3 4 8 [Reporter]SCO1841 (14H14)_PCR/5617 [Gene]SCO1841/5121 NC_003888 SCI8.26, unknown, len: 194 aa. Weakly similar to a protein of unknown function from Mycobacterium tuberculosis: SW:YZ15_MYCTU (EMBL: Z73101) hypothetical 16.1 KD protein precursor (152 aa), fasta scores opt: 220 z-score: 256.4 E(): 6.5e-07 33.6% identity in 131 aa overlap. 4 3 4 7 [Reporter]SCO3924 (15D14)_PCR/5616 [Gene]SCO3924/5120 NC_003888 SCQ11.07, possible membrane protein, len: 70 aa; previously sequenced as SW:YPT1_STRCO (EMBL:U37580) hypothetical protein in PtpA 5' region (64 aa), with different putative stop codon. Probable hydrophobic CDS suggested by GC frameplot and positional base preference. Contains possible hydrophobic membrane spanning regions 4 3 4 6 [Reporter]SCO1031 (16P10)_PCR/5615 [Gene]SCO1031/5119 NC_003888 SCG20A.11c, possible ABC transport system integral membrane protein, len: 855 aa; similar to TR:CAB89462 (EMBL:AL354048) Streptomyces coelicolor putative ABC transporter integral membrane protein SCE25.31, 843 aa; fasta scores: opt: 1459 z-score: 1443.9 E(): 0; 34.7% identity in 864 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 4 5 [Reporter]SCO0103 (17L10)_PCR/5614 [Gene]SCO0103/5118 NC_003888 SCJ11.32, probable flavohemoprotein, len: 435aa; C-terminal region similar to many with reductase and dioxygenase activities, typical of flavohemoproteins e.g. TR:Q9ZI75 (EMBL:AF039533) naphthalene dioxygenase reductase from Pseudomonas stutzeri (328 aa) fasta scores; opt: 369, z-score: 419.0, E(): 5.6e-16, (28.6% identity in 241 aa overlap). Also typical of flavohemoprotein, C-terminal region contains Pfam match to entry PF00175 oxidored_fad, Oxidoreductase FAD/NAD-binding domain and N-terminal region contains Pfam match to entry PF00042 globin, Globin. 4 3 4 4 [Reporter]SCO0748 (18H10)_PCR/5613 [Gene]SCO0748/5117 NC_003888 SCF81.07, possible hydrolase, len: 193 aa; similar to TR:P94573 (EMBL:Z82987) Bacillus subtilis hypothetical 21.1KD protein, 189 aa; fasta scores: opt: 504 z-score: 582.8 E(): 4.4e-25; 48.4% identity in 190 aa overlap and to SW:ENTB_ECOLI (EMBL:M24148) Escherichia coli isochorismatase (EC 3.3.2.1) (2,3 dihydro-2,3 dihydroxybenzoate synthase) EntB, 285 aa; fasta scores: opt: 205 z-score: 242.1 E(): 4.1e-06; 26.8% identity in 183 aa overlap. Contains match to Pfam entry PF00857 Isochorismatase, Isochorismatase family 4 3 4 3 [Reporter]SCO1290 (19D10)_PCR/5612 [Gene]SCO1290/5116 NC_003888 SCBAC36F5.01, possible secreted phosphatase (fragment), len: >296 aa; similar to C-terminal region of SW:PPBD_BACSU (EMBL:U49060) Bacillus subtilis alkaline phosphatase D precursor (EC 3.1.3.1) PhoD, 556 aa; fasta scores: opt: 277 Z-score: 319.4 bits: 68.0 E(): 3.5e-10; 30.258% identity in 271 aa overlap,2SCG18.37, possible secreted alkaline phosphatase (fragment), len: >266 aa; similar to SW:PPBD_BACSU (EMBL:U49060) Bacillus subtilis alkaline phosphatase D precursor (EC 3.1.3.1) PhoD, 556 aa; fasta scores: opt: 148 z-score: 173.1 E(): 0.037; 25.2% identity in 270 aa overlap. Contains possible N-terminal region signal peptide sequence 4 3 4 2 [Reporter]SCO6312 (20L6)_PCR/5611 [Gene]SCO6312/5115 NC_003888 SCIF3.14c, cprA, transcriptional regulator, len: 215 aa; identical to previously sequenced TR:O66121 (EMBL:AB000384) Streptomyces coelicolor CprS, 215 aa. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 30..51 (+3.61 SD) 4 3 3 22 [Reporter]SCO4543 (1L2)_PCR/5609 [Gene]SCO4543/5114 NC_003888 2SCD4.14c, hypothetical protein, len: 283 aa; identical to previously sequenced TR:Q9XD96 (EMBL:AF106004) Streptomyces coelicolor hypothetical 31.8 kDa protein, 283 aa 4 3 3 21 [Reporter]SCO0735 (2H2)_PCR/5608 [Gene]SCO0735/5113 NC_003888 3SC5B7.13, possible oxidoreductase, len: 400 aa; low similarity to TR:Q9KDG5 (EMBL:AP001511) Bacillus halodurans NADH-dependent dehydrogenase BH1248, 340 aa; fasta scores: opt: 243 z-score: 286.8 E(): 1.9e-08; 24.8% identity in 242 aa overlap. Contains Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family 4 3 3 20 [Reporter]SCO1621 (3D2)_PCR/5607 [Gene]SCO1621/5112 NC_003888 SCI41.04c, opuAA glycine betaine transport ATP-binding protein, len: 364 aa. Highly similar to SW:OPUA_BACSU (P46920) Bacillus subtilis glycine betaine transport ATP-binding protein; fasta scores: opt: 1093 z-score: 1200.6 E():0; 52.4% identity in 328 aa overlap. Contains Pfam match to PF00005 ABC_tran, ABC transporter and PS00211 ABC transporters family signature. 4 3 3 18 [Reporter]SCO2024 (4L22)_PCR/5605 [Gene]SCO2024/5111 NC_003888 SC3A3.02, probable chitosanase (putative secreted protein), len: 305 aa; similar to chitosanases e.g. SW:CHIS_STRSQ (EMBL:L07779), csn, Streptomyces sp. (strain N174) chitosanase precursor (278 aa), fasta scores; opt: 893 z-score: 1036.3 E(): 0, 58.4% identity in 233 aa overlap. Has an approx. 70 aa N-terminal extension not present in homologues which includes a possible N-terminal signal sequence. Contains Pfam match to entry PF01374 Glyco_hydro_46, Glycosyl hydrolase family 46 4 3 3 17 [Reporter]SCO7133 (5H22)_PCR/5604 [Gene]SCO7133/5110 NC_003888 SC4B10.34, possible membrane protein, len: 113 aa; similar to TR:CAB89768 (EMBL:AL354616) Streptomyces coelicolor putative membrane protein SC3A4.22, 114 aa; fasta scores: opt: 276 z-score: 371.5 E(): 3.1e-13; 39.4% identity in 109 aa overlap. Contains possible hydrophobic membrane spanning region 4 3 3 16 [Reporter]SCO7587 (6D22)_PCR/5603 [Gene]SCO7587/5109 NC_003888 SC5F1.41, possible integral membrane protein, len: 455 aa; similar to TR:Q9L0X9 (EMBL:AL159178) Streptomyces coelicolor putative membrane protein SCH22A.03c, 429 aa; fasta scores: opt: 1974 z-score: 2163.7 E(): 0; 69.7% identity in 423 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 3 15 [Reporter]SCO4409 (7P18)_PCR/5602 [Gene]SCO4409/5108 NC_003888 SC6F11.07c, possible RNA polymerase sigma factor, len: 197 aa; similar to SW:SIGZ_BACSU (EMBL:U93875) Bacillus subtilis RNA polymerase sigma factor SigZ, 176 aa; fasta scores: opt: 166 z-score: 208.5 E(): 0.00041; 27.0% identity in 148 aa overlap 4 3 3 14 [Reporter]SCO7592 (8L18)_PCR/5601 [Gene]SCO7592/5107 NC_003888 SC7H9.04, hypothetical protein, len: 297 aa; similar to TR:Q9RCY4 (EMBL:AL133469) Streptomyces coelicolor hypothetical 31.4 kDa protein SCM10.14c, 299 aa; fasta scores: opt: 791 z-score: 890.7 E(): 0; 46.4% identity in 302 aa overlap 4 3 3 13 [Reporter]SCO7148 (9H18)_PCR/5600 [Gene]SCO7148/5106 NC_003888 SC9A4.10, hypothetical protein, len: 117 aa; similar to C-terminal region of SW:DAC_STRSQ (EMBL:M26842) Streptomyces sp. D-alanyl-D-alanine carboxypeptidase precursor (EC 3.4.16.4) Dac, 406 aa; fasta scores: opt: 186 z-score: 224.1 E(): 5.1e-05; 43.1% identity in 102 aa overlap 4 3 3 12 [Reporter]SCO2457 (10D18)_PCR/5599 [Gene]SCO2457/5105 NC_003888 SCC24.28c, possible lipoprotein, len: 131 aa; similar to SW:Y0B2_MYCTU (EMBL:Z77724) Mycobacterium tuberculosis hypothetical 15.0 kD protein CY227.02c precursor, MTCY227.02c, 143 aa; fasta scores: opt: 130 z-score: 169.2 E(): 0.054; 26.5% identity in 136 aa overlap. Contains match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 3 3 11 [Reporter]SCO4503 (11P14)_PCR/5598 [Gene]SCO4503/5104 NC_003888 SCD35.10, probable long-chain-fatty acid CoA ligase, len: 642 aa; similar to TR:Q9ZBW6 (EMBL:AL034443) Streptomyces coelicolor putative long-chain-fatty acid CoA ligase SC4B5.02c, 608 aa; fasta scores: opt: 2125 z-score: 2339.4 E(): 0; 53.0% identity in 610 aa overlap and to SW:LCFE_RAT (EMBL:AB012933) Rattus norvegicus long-chain-fatty acid CoA ligase 5 (EC 6.2.1.3) FacL5, 683 aa; fasta scores: opt: 488 z-score: 536.4 E(): 1.9e-22; 27.3% identity in 607 aa overlap. Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and match to Prosite entry PS00455 Putative AMP-binding domain signature 4 3 3 10 [Reporter]SCO3288 (12L14)_PCR/5597 [Gene]SCO3288/5103 NC_003888 SCE15.05, possible integral membrane protein, len: 243aa; similar to TR:Q53715 (EMBL:L06249) hypothetical protein from Streptomyces antibioticus (234 aa) fasta scores; opt: 292, z-score: 298.6, E(): 2.5e-09, (33.2% identity in 241 aa overlap). Contains a possible membrane spanning hydrophobic region and a 8x5 degenerate repeat of GT***. 4 3 3 8 [Reporter]SCO0934 (14D14)_PCR/5595 [Gene]SCO0934/5102 NC_003888 SCM10.22c, possible integral membrane protein, len: 213 aa. Contains possible hydrophobic membrane spanning regions 4 3 3 7 [Reporter]SCO1697 (15P10)_PCR/5594 [Gene]SCO1697/5101 NC_003888 SCI30A.18c, probable merR-family transcriptional regulator, len: 175aa; similar to many egs. TR:CAA09641 (EMBL:AJ011500) from Streptomyces violaceoruber Tu22 (196 aa) fasta scores; opt: 698, z-score: 833.1, E(): 0, (64.1% identity in 167 aa overlap) and SW:SOXR_ECOLI SoxR transcriptional regulator from Escherichia coli (154 aa) fasta scores; opt: 575, z-score: 689.9, E(): 4.3e-31, (60.3% identity in 146 aa overlap). Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family. 4 3 3 6 [Reporter]SCO0069 (16L10)_PCR/5593 [Gene]SCO0069/5100 NC_003888 SCJ4.50, unknown, len: 1052 aa 4 4 1 15 [Reporter]SCO2667 (7H17)_PCR/7573 [Gene]SCO2667/6899 NC_003888 SC6D10.10, unknown, len: 482 aa 4 4 1 14 [Reporter]SCO6855 (8D17)_PCR/7572 [Gene]SCO6855/6898 NC_003888 SC7F9.07c, unknown, len: 127 aa. Weakly similar to SC7F9.08c hypothetical protein, also located on this cosmid (215 aa), fasta scores opt:98 z-score: 114.2 E(): 0.66 28.9% identity in 121 aa overlap. 4 4 1 13 [Reporter]SCO5845 (9P13)_PCR/7571 [Gene]SCO5845/6897 NC_003888 SC9B10.12, possible thimidine kinase, len: 216 aa; similar to several eg. KITH_ECOLI P23331 thymidine kinase (ec 2.7.1.21) (205 aa), fasta scores; opt: 404 z-score: 500.0 E(): 1.1e-20, 35.5% identity in 200 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 4 4 1 12 [Reporter]SCO2320 (10L13)_PCR/7570 [Gene]SCO2320/6896 NC_003888 SCC53.11c, hypothetical protein, len: 294 aa; similar to regions from many larger proteins eg. TR:O54230 (EMBL:AJ000264) protein kinase (EC 2.7.1.37) from Streptomyces granaticolor (701 aa) fasta scores; opt: 160, z-score: 170.0, E(): 0.054, 29.5% identity in 190 aa overlap and SW:P54739 (PKAA_STRCO) serine/threonine protein kinase PkaA from Streptomyces coelicolor (543 aa) fasta scores; opt: 129, z-score: 139.5, E(): 2.7, 31.7% identity in 139 aa overlap. Contains TTA leucine codon, possible target for bldA regulation. 4 4 1 11 [Reporter]SCO4549 (11H13)_PCR/7569 [Gene]SCO4549/6895 NC_003888 SCD16A.34c, possible type IV peptidase, len: 259aa; similar to many eg. SW:LEP3_PSEAE type IV prepilin peptidase from Pseudomonas aeruginosa (290 aa) fasta scores; opt: 304, z-score: 344.8, E(): 7e-12, (32.5% identity in 228 aa overlap). Contains possible hydrophobic membrane spanning regions 4 4 1 10 [Reporter]SCO4234 (12D13)_PCR/7568 [Gene]SCO4234/6894 NC_003888 SCD8A.07, possible 2C-methyl-D-erythriol 2,4-cyclodiphosphate synthase, len: 170 aa; similar to various hypothetical proteins, e.g. TR:P96863 (EMBL:Z92774) Mycobacterium tuberculosis hypothetical 16.4 kD protein MCTY06G11.28c, 159 aa; fasta scores: opt: 585 z-score: 695.0 E(): 2.8e-31; 56.5% identity in 154 aa overlap and to many putatively involved in isoprenoid biosynthesis, e.g. SW:ISPF_ECOLI (EMBL:AF230738) Escherichia coli 2C-methyl-D-erythriol 2,4-cyclodiphosphate synthase (MECPS) IspF or MecS or B2746, 159 aa; fasta scores: opt: 326 Z-score: 385.1 E(): 8.1e-14; 36.538% identity in 156 aa overlap 4 4 1 9 [Reporter]SCO3110 (13P9)_PCR/7567 [Gene]SCO3110/6893 NC_003888 SCE41.19c, possible ABC transport system integral membrane protein, len: 854 aa; similar to TR:CAB96016 (EMBL:AL360055) Streptomyces coelicolor putative ABC transport system integral membrane protein SCG20A.11c, 855 aa; fasta scores: opt: 1429 z-score: 1454.6 E(): 0; 34.0% identity in 871 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 1 8 [Reporter]SCO1540 (14L9)_PCR/7566 [Gene]SCO1540/6892 NC_003888 SCL2.30c, possible membrane protein, len: 150 aa; similar to SW:YAIZ_ECOLI (EMBL:AE000144) Escherichia coli hypothetical 13.0 kD protein YaiZ, 114 aa; fasta scores: opt: 121 z-score: 164.5 E(): 0.09; 31.0% identity in 71 aa overlap and to TR:CAB53332 (EMBL:AL109974) Streptomyces coelicolor putative membrane protein SCF34.21, 144 aa; fasta scores: opt: 117 z-score: 158.1 E(): 0.21; 23.9% identity in 92 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 1 7 [Reporter]SCO2866 (15H9)_PCR/7565 [Gene]SCO2866/6891 NC_003888 SCE6.03, unknown, len: 84 aa. 4 4 1 6 [Reporter]SCO3854 (16D9)_PCR/7564 [Gene]SCO3854/6890 NC_003888 SCH69.24, putative membrane protein, len: 84 aa; similar to SW:YO11_MYCTU hypothetical protein from Mycobacterium tuberculosis (93 aa) fasta scores; opt: 169, z-score: 235.0, E(): 9.2e-06, (31.5% identity in 92 aa overlap) and highly similar to TR:CAC47962 (EMBL:AJ320280) Streptomyces avermitilis WhiP protein, 84 aa; fasta scores: pt: 536 Z-score: 670.7 E(): 1e-29; 92.857% identity in 84 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 1 5 [Reporter]SCO3757 (17P5)_PCR/7563 [Gene]SCO3757/6889 NC_003888 SCH63.04c, possible two component system sensor kinase, len: 448 aa; similar to TR:Q9X8Q6 (EMBL:AL049754) Streptomyces coelicolor putative two-component system sensor kinase SCH10.17c, 472 aa; fasta scores: opt: 595 z-score: 616.4 E(): 8.2e-27; 36.4% identity in 453 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 1 4 [Reporter]SCO3308 (18L5)_PCR/7562 [Gene]SCO3308/6888 NC_003888 SCE68.06c, probable DNA-binding protein, len: 464 aa; similar to TR:Q9AK04 (EMBL:AL512944) Streptomyces coelicolor hypothetical protein SC8D11.15c, 471 aa; fasta scores: opt: 428 Z-score: 331.6 E(): 7.7e-11; 40.377% identity in 478 aa overlap; probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains a hydrophobic, possible membrane-spanning region, followed by a low-complexity proline- and serine-rich region from aa 210 to 250 approx. Contains probable helix-turn-helix motif at aa 20-41 (Score 1605, +4.65 SD) 4 4 1 3 [Reporter]SCP1.153 (19H5)_PCR/7561 [Gene]SCP1.153/6887 NC_003888 SCP1.153, possible secreted protein, len: 157aa; contains a possible N-terminal signal sequence. 3 4 23 22 [Reporter]SCO1753 (1K21)_PCR/7559 [Gene]SCO1753/6886 NC_003888 2SCI34.06, possible integral membrane protein, len: 340 aa. Contains Pfam match to entry PF01569 PAP2, PAP2 superfamily and possible hydrophobic membrane spanning regions. Also presents high content of alanine, arginine, leucine and proline amino acid residues 3 4 23 21 [Reporter]SCO3874 (2G21)_PCR/7558 [Gene]SCO3874/6885 NC_003888 SCH18.11c, gyrB, DNA gyrase subunit B, len: 686 aa; identical to previously sequenced to SW:GYRB_STRCO (EMBL:L27063) Streptomyces coelicolor DNA gyrase subunit B (EC 5.99.1.3) GyrB, 686 aa and similar to TR:O69998 (EMBL:AL022374) S. coelicolor gyrB2 SC5B8.12, 70 aa; fasta scores: opt: 1227 z-score: 1210.5 E(): 0; 44.7% identity in 711 aa overlap. Contains Pfam matches to entries PF00204 DNA_topoisoII, DNA topoisomerase II (N-terminal region) and PF00986 DNA_gyraseB_C, DNA gyrase B subunit, carboxyl terminus and match to Prosite entry S00177 DNA topoisomerase II signature 3 4 23 20 [Reporter]SCO7381 (3C21)_PCR/7557 [Gene]SCO7381/6884 NC_003888 SC10G8.08, unknown, len: 137 aa. 3 4 23 19 [Reporter]SCO7605 (4O17)_PCR/7556 [Gene]SCO7605/6883 NC_003888 SC2H2.03c, possible metallopeptidase, len: 473 aa; low similarity to TR:Q9X7P2 (EMBL:AL049587) Streptomyces coelicolor putative metallopeptidase SC5F2A.19c, 506 aa; fasta scores: opt: 226 z-score: 242.7 E(): 5.5e-06; 23.7% identity in 363 aa overlap. Contains Pfam match to entry PF01433 Peptidase_M1, Peptidase family M1 and match to Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Contains possible hydrophobic membrane spanning region 3 4 23 18 [Reporter]SCO6697 (5K17)_PCR/7555 [Gene]SCO6697/6882 NC_003888 SC4C6.07c, pcaL, probable 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase, len: 375 aa; similar to TR:O67982 (EMBL:AF003947), pcaL, Rhodococcus opacus 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase (400 aa), fasta scores; opt: 401 z-score: 464.7 E(): 1.5e-18, 46.3% identity in 387 aa overlap the first approx. 260 aa is similar to SW:ELH2_ACICA (EMBL:AF009224), catD, Acinetobacter calcoaceticus 3-oxoadipate enol-lactonase II (266 aa) (35.1% identity in 248 aa overlap) while the remainder is similar to SW:DC4C_ACICA (EMBL:L05770), pcaC, 4-carboxymuconolactone decarboxylase. Highly similar to TR:O86608 (EMBL:AL031155) S.coelicolor probable 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase (449 aa) (43.3% identity in 439 aa overlap) which contains an approx. 70 residue insertion between the two domains of homology to the Acinetobacter enzymes. An alternative start codon is present at codon 4. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 3 4 23 17 [Reporter]SCO7549 (6G17)_PCR/7554 [Gene]SCO7549/6881 NC_003888 SC5F1.03c, hypothetical protein, len: 114 aa; similar to TR:Q9KXH9 (EMBL:AL357591) Streptomyces coelicolor hypothetical 20.1 kDa protein SCC53.32c, 187 aa; fasta scores: opt: 288 z-score: 334.7 E(): 4.1e-11; 46.4% identity in 97 aa overlap 3 4 23 16 [Reporter]SCO2662 (7C17)_PCR/7553 [Gene]SCO2662/6880 NC_003888 SC6D10.05, possible kinase, len: 305 aa; similar to TR:CAB61848 (EMBL:AJ242910) Homo sapiens N-acetylglucosamine kinase protein (EC 2.7.1.59), 344 aa; fasta scores: opt: 192 z-score: 223.6 E(): 4.8e-05; 24.5% identity in 253 aa overlap and to TR:CAB61849 (EMBL:AJ242909) Mus musculus N-acetylglucosamine kinase protein (EC 2.7.1.59), 343 aa; fasta scores: opt: 180 z-score: 210.1 E(): 0.00027; 24.4% identity in 250 aa overlap 3 4 23 15 [Reporter]SCO1988 (8O13)_PCR/7552 [Gene]SCO1988/6879 NC_003888 SC7H2.02, possible acetyltransferase, len: 183 aa; similar to SW:YIAC_ECOLI hypothetical protein from Escherichia coli (146 aa) fasta scores; opt: 178, z-score: 227.9, E(): 2.3e-05, (27.2% identity in 147 aa overlap). Also has region of similarity to several acetyltransferases eg. TR:Q08414 (EMBL:X73149) nourseothricin acetyltransferase from Streptomyces noursei (189 aa) fasta scores; opt: 115, z-score: 150.4, E(): 0.47, (40.2% identity in 82 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 3 4 23 13 [Reporter]SCO1928 (10G13)_PCR/7550 [Gene]SCO1928/6878 NC_003888 SCC22.10, possible ABC transporter, ATP-binding subunit, len: 307aa; similar to many egs. SW:DRRA_STRPE daunorubicin resistance ATP-binding protein from Streptomyces peucetius (330 aa) fasta scores; opt: 571, z-score: 637.1, E(): 3.7e-28, (35.6% identity in 315 aa overlap) and TR:O53149 (EMBL:AL021184) putative ABC transporter, ATP-binding protein from Mycobacterium tuberculosis (313 aa) fasta scores; opt: 1170, z-score: 1297.6, E(): 0, (60.9% identity in 304 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. 3 4 23 12 [Reporter]SCO4554 (11C13)_PCR/7549 [Gene]SCO4554/6877 NC_003888 SCD16A.29c, possible bi-functional transferase/deacetylase, len: 743aa; 380-620aa region similar to TR:BAA35803 (EMBL:D90739) glycosyl transferase from Escherichia coli (441 aa) fasta scores; opt: 518, z-score: 576.7, E(): 8.6e-25, (35.4% identity in 257 aa overlap). 100-290aa region similar to SW:NODB_RHIME chitooligosaccharide deactylase from Rhizobium meliloti (217 aa) fasta scores; opt: 398, z-score: 448.0, E(): 1.3e-17, (35.7% identity in 196 aa overlap). Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases. Also contains possible membrane spanning hydrophobic regions. 3 4 23 10 [Reporter]SCO3105 (13K9)_PCR/7547 [Gene]SCO3105/6876 NC_003888 SCE41.14, unknown, len: 201 aa 3 4 23 9 [Reporter]SCO1075 (14G9)_PCR/7546 [Gene]SCO1075/6875 NC_003888 SCG22.21c, possible bifunctional protein (ATP/GTP binding protein/MutT-like), len: 347 aa; N-terminal domain similar to TR:O88042 (EMBL:AL031541) Streptomyces coelicolor ATP/GTP binding protein SCI35.04c, 174 aa; fasta scores: opt: 462 z-score: 521.1 E(): 1.5e-21; 47.2% identity in 180 aa overlap and C-terminal domain similar to TR:CAB85162 (EMBL:AL162757) Neisseria meningitidis MutT-like protein NMA1942, 174 aa; fasta scores: opt: 148 z-score: 174.6 E(): 0.03; 28.9% identity in 121 aa overlap. Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 4 23 8 [Reporter]SCO3699 (15C9)_PCR/7545 [Gene]SCO3699/6874 NC_003888 SCH35.25, probable transcriptional regulator, len: 102aa; similar to many eg. SW:ARSR_ECOLI arsenical resistance operon from Escherichia coli (117 aa) fasta scores; opt: 147, z-score: 212.9, E(): 0.00016, (36.6% identity in 71 aa overlap). Contains Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family. 3 4 23 7 [Reporter]SCO3139 (16O5)_PCR/7544 [Gene]SCO3139/6873 NC_003888 SCE66.18, possible sodium:solute symporter, len: 562aa; similar to many eg. SW:P96169 (SGLT_VIBPA) sodium/glucose co-transporter from Vibrio parahaemolyticus (543 aa) fasta scores; opt: 538, z-score: 594.1, E(): 1.3e-25, 27.8% identity in 479 aa overlap. Contains Pfam match to entry PF00474 SSF, Sodium:solute symporter family and possible membrane-spanning hydrophobic regions. 3 4 23 6 [Reporter]SCO6497 (17K5)_PCR/7543 [Gene]SCO6497/6872 NC_003888 SC1E6.06, tktA2, probable transketolase, len: 615 aa; highly similar to eukaryotic transketolases e.g. TKT_HUMAN transketolase (EC 2.2.1.1) (623 aa), fasta scores; opt: 1909 z-score: 2169.3 E(): 0, 50.0% identity in 614 aa overlap; less similar to prokaryotic transketolases e.g. TKT_RHOSH transketolase (657 aa), fasta scores; opt: 477 z-score: 830.4 E(): 0, 31.3% identity in 635 aa overlap. Contains PS00802 Transketolase signature 2 and Pfam match to entry PF00456 transketolase, Transketolase, score 397.70, E-value 1.1e-115. Also similar to S. coelicolor tkt (SC5A7.13c) E(): 4.2e-20, 29.0% identity in 679 aa overlap 3 3 6 4 [Reporter]SCO2373 (18O10)_PCR/5153 [Gene]SCO2373/4699 NC_003888 SCC8A.31c, tcmA, tetracenomycin C efflux protein, partial CDS, len: >331aa; similar to many export protein especially SW:TCMA_STRGA( EMBL:M80674) tetracenomycin C resistance and export protein from Streptomyces glaucescens (538 aa) fasta scores; opt: 1285, z-score: 1299.8, E(): 0, 59.8% identity in 323 aa overlap.,SC1E12.01c, tcmA, tetracenomycin C efflux protein, partial CDS, len: >103 aa. Highly similar over the available sequence to Streptomyces glaucescens SW:TCMA_STRGA(EMBL:M80674) tetracenomycin C resistance and export protein (538 aa), fasta scores opt: 500 z-score: 635.7 E(): 5e-28 70.9% identity in 103 aa overlap. Also similar to several from Streptomyces coelicolor: SW:AC22_STRCO(EMBL:M64683) probable actinorhodin transporter ACTII-2 (578 aa), fasta scores opt: 246 z-score: 316.7 E(): 2.9e-10 35.8% identity in 109 aa overlap and SCF37.21c (EMBL:AL133210) probable export protein (533 aa), fasta scores opt: 378 z-score: 434.6 E(): 8e-19 47.6% identity in 103 aa overlap. Contains possible membrane spanning hydrophobic domains.,SC4A7.01c, tcmA, tetracenomycin C resistance and export protein (partial CDS), len: >173 aa; highly similar to SW:TCMA_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C resistance and export protein TcmA, 538 aa; fasta scores: opt: 813 z-score: 922.3 E(): 0; 78.6% identity in 159 aa overlap, to SW:AC22_STRCO (EMBL:M64683) Streptomyces coelicolor probable actinorhidin transporter ActII-2, 578 aa; fasta scores: opt: 396 z-score: 452.6 E(): 7.9e-18; 37.8% identity in 164 aa overlap and to S. coelicolor SCF37.21c, 533 aa; fasta scores: opt: 641 z-score: 623.5 E(): 2.4e-29; 56.7% identity in 171 aa overlap 3 4 23 5 [Reporter]SCO0594 (18G5)_PCR/7542 [Gene]SCO0594/6871 NC_003888 SCF55.18c, possible methyltransferase, len: 246 aa; similar to TR:CAB48912 (EMBL:AL096837) Streptomyces ceolicolor putative methyltransferase SCF43A.25c, 215 aa; fasta scores: opt: 190 z-score: 231.6 E(): 1.6e-05; 34.9% identity in 126 aa overlap and to SW:BIOC_SERMA biotin synthesis protein, BioC, 255 aa; fasta scores: opt: 179 z-score: 217.7 E(): 9.4e-05; 32.3% identity in 130 aa overlap 3 3 6 3 [Reporter]SCO1361 (19K10)_PCR/5152 [Gene]SCO1361/4698 NC_003888 SC10A9.03, conserved hypothetical protein, len: 228 aa; similar to TR:Q9RW27 (EMBL:AE001938) Deinococcus radiodurans NimA-related protein DR0842, 195 aa; fasta scores: opt: 210 z-score: 256.7 E(): 7.4e-07; 30.5% identity in 197 aa overlap and to TR:Q98JR3 (EMBL:AP002998) Rhizobium loti (Mesorhizobium loti) MLL1820 protein, 251 aa; fasta scores: opt: 589 Z-score: 692.3 E(): 6.3e-31; 42.478% identity in 226 aa overlap 3 4 23 4 [Reporter]SCO1966 (19C5)_PCR/7541 [Gene]SCO1966/6870 NC_003888 SCC54.26c, partial CDS, probable uvrB, ABC excision nuclease subunit B, len: >113aa; similar to many eg. SW:UVRB_MICLU uvrB, ABC excision nuclease subunit B from Micrococcus luteus (709 aa) fasta scores; opt: 248, z-score: 280.5, E(): 2.6e-08, (45.9% identity in 111 aa overlap).,SC3C9.01c, uvrB, excinuclease ABC subunit B (fragment), len: >635 aa; highly similar to SW:UVRB_BACSU (EMBL:AF017113) Bacillus subtilis excinuclease ABC subunit B (DinA protein) UvrB or DinA or Uvr aa; fasta scores: opt: 2591 Z-score: 2687.6 bits: 507.5 E(): 4.3e-142; 63.667% identity in 600 aa overlap. Contains Pfam matches to entries PF00270 DEAD, DEAD/DEAH box helicase and PF00271 helicase_C, Helicase conserved C-terminal domain and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Also contains a possible coiled-coil region at approx. residues 273..290 3 3 6 2 [Reporter]SCP1.170 (20C10)_PCR/5151 [Gene]SCP1.170/4697 NC_003888 SCP1.170, possible integral membrane protein, len: 330aa; similar to TR:Q9L0R7 (EMBL:AL160312) putative integral membrane protein from Streptomyces coelicolor (345 aa) fasta scores; opt: 385, z-score: 452.0, E(): 1e-17, 29.8% identity in 292 aa overlap. Contains possible membrane-spanning hydrophobic regions. 3 3 5 22 [Reporter]SCO5387 (1C6)_PCR/5149 [Gene]SCO5387/4696 NC_003888 2SC6G5.31, unknown, len: 874 aa 3 3 5 21 [Reporter]SCO1245 (2O2)_PCR/5148 [Gene]SCO1245/4695 NC_003888 2SCG1.20, bioA, adenosylmethionine-8-amino-7-oxononanoate aminotransferase, len: 403 aa; similar to SW:BIOA_ECOLI (EMBL:J04423) Escherichia coli adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) BioA, 429 aa; fasta scores: opt: 1509 z-score: 1711.2 E(): 0; 54.5% identity in 420 aa overlap. Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate and match to Prosite entry PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site 3 3 5 20 [Reporter]SCO4649 (3K2)_PCR/5147 [Gene]SCO4649/4694 NC_003888 SCD82.20, rlpA, 50S ribosomal protein L1, len: 241 aa; identical to previously sequenced SW:RL1_STRGR (EMBL:X72787) Streptomyces griseus 50S ribosomal protein L1 RlpA, 240 aa. Contains Pfam match to entry PF00687 Ribosomal_L1, L1P family of ribosomal proteins and match to Prosite entry PS01199 Ribosomal protein L1 signature 3 3 5 19 [Reporter]SCO6898 (4G2)_PCR/5146 [Gene]SCO6898/4693 NC_003888 SC1B2.04, possible carboxylase, len: 405 aa. Weakly similar to several e.g. Anabaena sp. (strain PCC 7120) SW:ACCC_ANASP(EMBL:L14862) biotin carboxylase (EC 6.3.4.14) (447 aa), fasta scores opt: 226 z-score: 262.2 E(): 3.8e-07 28.5% identity in 214 aa overlap. Contains a Pfam match to entry PF00289 CPSase_L_chain, Carbamoyl-phosphate synthase (CPSase) and a Prosite hit to PS00867 Carbamoyl-phosphate synthase subdomain signature 2. 3 3 5 18 [Reporter]SCO6336 (5C2)_PCR/5145 [Gene]SCO6336/4692 NC_003888 SC3A7.04, questionable ORF, len: 384 aa; this ORF is not predicted as coding by positional base preference 3 3 5 17 [Reporter]SCO7062 (5O22)_PCR/5144 [Gene]SCO7062/4691 NC_003888 SC4G1.28, possible methylase, len: 280 aa; similar to TR:P72542 (EMBL:U60417) Streptomyces pristinaespiralis PapM, 292 aa; fasta socres: opt: 401 z-score: 442.7 E(): 3.4e-17; 35.2% identity in 264 aa overlap and to SW:HEMK_ECOLI (EMBL:D28567) Escherichia coli HemK protein, 277 aa; fasta scores: opt: 299 z-score: 332.7 E(): 4.6e-11; 32.3% identity in 254 aa overlap 3 3 5 16 [Reporter]SCO4833 (6K22)_PCR/5143 [Gene]SCO4833/4690 NC_003888 SC5G8.01c, partial CDS, possible phosphorylmutase, len: >101 aa; similar to many e.g. SW:P11435 (CPPM_STRHY) carboxyvinyl-carboxyphosphonate phosphorylmutase from Streptomyces hygroscopicus (294 aa) fasta scores; opt: 185, z-score: 234.7, E(): 1.3e-05, 43.0% identity in 93 aa overlap.,SC2A6.18c, possible transferase (fragment), len: >172 aa; similar to C-terminal part of SW:CPPM_BACSU (EMBL:D84432) Bacillus subtilis putative carboxyvinyl-carboxyphosphonate phosphorylmutase (EC 2.7.8.23) YqiQ, 301 aa; fasta scores: opt: 158 z-score: 187.0 E(): 0.0062; 31.0% identity in 116 aa overlap 2 1 17 16 [Reporter]SCO5655 (7J12)_PCR/871 [Gene]SCO5655/799 NC_003888 SC6A9.12, probable aminotransferase, len: 457 aa; similar to many e.g. BIOA_BACSU adenosylmethionine-8-amino-7-oxononanoate aminotransferase (448 aa), fasta scores; opt: 895 z-score: 881.6 E(): 0, 35.6% identity in 449 aa overlap. Also highly similar to M. tuberculosis putative aminotransferase TR:O53379 (EMBL:AL021841) MTV016.29; 70.3% identity in 438 aa overlap. Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate, score 355.70, E-value 3.5e-123 2 1 17 15 [Reporter]SCO5290 (8F12)_PCR/870 [Gene]SCO5290/798 NC_003888 SC6G9.43c, cvnB5, conserved hypothetical protein, len: 150 aa; similar to hypothetical proteins from S. coelicolor and Mycobacterium tuberculosis e.g. TR:O86524 (EMBL:AL031124) S.coelicolor hypothetical protein (173 aa), fasta scores; opt: 316 z-score: 394.7 E(): 1.2e-14, 48.1% identity in 131 aa overlap and TR:O50393 (EMBL:AL009198) Mycobacterium tuberculosis hypothetical protein (130 aa) (36.7% identity in 120 aa overlap) 2 1 17 14 [Reporter]SCO2628 (9B12)_PCR/869 [Gene]SCO2628/797 NC_003888 SC8E4.03, probable amino acid permease, len: 422 aa; similar to SW:GABP_BACSU (EMBL:U31756) Bacillus subtilis GABA permease (4-amino butyrate transport carrier) GabP, 469 aa; fasta scores: opt: 1479 z-score: 1502.0 E(): 0; 58.5% identity in 366 aa overlap, to SW:GABP_ECOLI (EMBL:M88334) Escherichia coli GABA permease (4-amino butyrate transport carrier) GabP, 466 aa; fasta scores: opt: 1334 z-score: 1355.6 E(): 0; 52.4% identity in 372 aa overlap and to S. ceolicolor SC8E4A.01 517 aa; fasta scores: opt: 1367 z-score: 1291.7 E(): 0; 54.4% identity in 371 aa overlap. Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease and match to Prosite entry PS00218 Amino acid permeases signature. Contains also several possible hydrophobic membrane spanning regions 2 1 17 13 [Reporter]SCO2519 (10N8)_PCR/868 [Gene]SCO2519/796 NC_003888 SCC121.22c, probable membrane protein, len: 692 aa; similar to SW:MMLA_STRCO (EMBL:M64683) Streptomyces coelicolor putative membrane protein ActII-3, 711 aa; fasta scores: opt: 1700 z-score: 1790.7 E(): 0; 42.7% identity in 693 aa overlap and to S. coelicolor SCF43.29c, 705 aa; fasta scores: opt: 848 z-score: 750.5 E(): 0; 40.1% identity in 700 aa overlap 2 1 17 12 [Reporter]SCO4395 (11J8)_PCR/867 [Gene]SCO4395/795 NC_003888 SCD10.27, possible hydrolase, len: 352 aa; similar to TR:Q9RCX1 (EMBL:AL133469) Streptomyces coelicolor putative hydrolase SCM10.27c, 287 aa; fasta scores: opt: 290 z-score: 298.8 E(): 3.6e-09; 28.5% identity in 277 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 2 1 17 11 [Reporter]SCO4470 (12F8)_PCR/866 [Gene]SCO4470/794 NC_003888 SCD65.13, probable phosphoglycerate mutase, len: 233 aa; similar to SW:PMG2_ECOLI (EMBL:M97495) Escherichia coli probable phosphoglycerate mutase 2 (EC 5.4.2.1) GpmB, 215 aa; fasta scores: opt: 182 z-score: 227.1 E(): 3.5e-05; 26.5% identity in 215 aa overlap. Contains Pfam match to entry PF00300 PGAM, Phosphoglycerate mutase family 2 1 17 10 [Reporter]SCO3090 (13B8)_PCR/865 [Gene]SCO3090/793 NC_003888 SCE25.31, possible ABC transporter integral membrane protein, len: 843 aa; similar to TR:CAB77000 (EMBL:AL159178) Streptomyces coelicolor putative integral membrane protein SCH22A.31, 850 aa; fasta scores: opt: 675 z-score: 676.0 E(): 3.4e-30; 26.4% identity in 879 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 17 9 [Reporter]SCO3751 (14N4)_PCR/864 [Gene]SCO3751/792 NC_003888 SCH22A.29, putative membrane protein, len: 89 aa. Contains possible hydrophobic membrane spanning regions 2 1 17 8 [Reporter]SCO3495 (15J4)_PCR/863 [Gene]SCO3495/791 NC_003888 SCE65.31c probable aldolase, len: 225 aa; similar to TR:AAD35160 (EMBL:AE001693) Thermotoga maritima 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase TM0066, 205 aa; fasta scores: opt: 437 z-score: 460.7 E(): 2.8e-18; 39.1% identity in 174 aa overlap and to SW:ALKH_BACSU (EMBL:L47838) Bacillus subtilis Khg/KdpG aldolase KdgA, 196 aa ;fasta scores: opt: 379 z-score: 401.8 E(): 5.3e-15; 37.9% identity in 177 aa overlap. Contains match to Pfam entry PF01081 Aldolase, KDPG and KHG aldolase 2 1 17 7 [Reporter]SCO4946 (16F4)_PCR/862 [Gene]SCO4946/790 NC_003888 2SCK31.06, hypothetical protein, len: 263 aa; highly similar to TR:Q9L114 (EMBL:AL159139) Streptomyces coelicolor hypothetical 27.0 kDa protein SCL6.02, 255 aa; fasta scores: opt: 711 z-score: 830.9 E(): 0; 49.4% identity in 253 aa overlap 3 4 23 3 [Reporter]SCP1.188c (20O1)_PCR/7540 [Gene]SCP1.188c/6869 NC_003888 SCP1.188c, unknown, len: 94aa; 3 4 22 22 [Reporter]SCO1141 (1G21)_PCR/7538 [Gene]SCO1141/6868 NC_003888 2SCG38.34c, conserved hypothetical protein, len: 140 aa; similar to SW:YP56_MYCTU (EMBL:Z77250) Mycobacterium tuberculosis hypothetical 13.9 kDa protein MTCY9C4.12, 129 aa; fasta scores: opt: 487 z-score: 626.4 E(): 1.9e-27; 61.2% identity in 121 aa overlap. Contains Pfam match to entry PF01894 UPF0047, Uncharacterised protein family UPF0047 3 4 22 21 [Reporter]SCO0649 (2C21)_PCR/7537 [Gene]SCO0649/6867 NC_003888 SCF91.09, gvpO2, possible gas vesicle synthesis protein, len: 105 aa. Similar in parts to several other gas vesicle synthesis proteins including: Halobacterium salinarium SW:GVO2_HALSA (EMBL:X64730) gas vesicle synthesis protein protein, chromosomal (111 aa), fasta scores opt: 205 z-score: 272.6 E(): 8.2e-08 39.0% identity in 77 aa overlap and Streptomyces coelicolor TR:Q9ZC14 (EMBL:AL033505) putative gas vesicle synthesis protein GvpO (130 aa), fasta scores opt: 223 z-score: 294.0 E(): 5.3e-09 48.7% identity in 76 aa overlap. 3 4 22 20 [Reporter]SCO6417 (3O17)_PCR/7536 [Gene]SCO6417/6866 NC_003888 SC1A6.06, possible integral membrane transporter, len: 522 aa; some similarity to e.g. FUR4_YEAST P05316 saccharomyces cerevisiae uracil permease (633 aa), fasta sores; opt:478 z-score: 454.0 E(): 3.6e-18, 28.2% identity in 485 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature 3 4 22 19 [Reporter]SCO7232 (4K17)_PCR/7535 [Gene]SCO7232/6865 NC_003888 SC2H12.31, possible secreted protein, len: 211 aa. Similar to Streptomyces coelicolor TR:CAB90893(EMBL:AL355753) putative lipoprotein, SC8G12.11 (187 aa), fasta scores opt: 176 z-score: 196.2 E(): 0.0019 32.8% identity in 189 aa overlap. Contains a possible N-terminal signal sequence. 3 4 22 17 [Reporter]SCO7789 (6C17)_PCR/7533 [Gene]SCO7789/6864 NC_003888 SC5E9.37c, unknown, len: 72 aa 3 4 22 16 [Reporter]SCO1420 (7O13)_PCR/7532 [Gene]SCO1420/6863 NC_003888 SC6D7.19c, possible integral membrane protein, len: 486 aa. Similar to several including: Bacillus subtilis SW:YXIO_BACSU(EMBL:D83026) hypothetical 47.3 KD protein (428 aa), fasta scores opt: 735 z-score: 858.7 E():0 29.7% identity in 434 aa overlap and Mycobacterium leprae TR:O07710(EMBL:Z97179) hypothetical 48.7 KD protein (464 aa), fasta scores opt: 327 z-score: 383.0 E(): 5.9e-14 29.7% identity in 445 aa overlap. Contains a TTA /leucine codon, possible target for bldA regulation and multiple possible membrane spanning hydrophobic domains. 3 4 22 15 [Reporter]SCO5962 (8K13)_PCR/7531 [Gene]SCO5962/6862 NC_003888 SC7H1.32c, probable transcriptional regulator, MerR family; len: 196 aa; similar to many eg. NOLA_BRASN P50330 nodulation protein nola. bradyrhizobium japonicum (237 aa), fasta scores; opt: 282 z-score: 360.0 E(): 7.4e-13, 34.6% identity in 159 aa overlap. Contains PS00552 Bacterial regulatory proteins, merR family signature, Helix-turn-helix motif at aa 10-31, Score 1627 (+4.73 SD), and Pfam match to entry merR PF00376, Bacterial regulatory proteins, merR family, score 92.85 3 3 5 15 [Reporter]SCO7434 (7G22)_PCR/5142 [Gene]SCO7434/4689 NC_003888 SC6D11.30, possible lipoprotein, len: 253 aa. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 3 4 22 14 [Reporter]SCO5417 (9G13)_PCR/7530 [Gene]SCO5417/6861 NC_003888 SC8F4.21, possible zinc-binding oxidoreductase, len: 339aa; similar to many proposed oxidoreductases eg. TR:O85702 (EMBL:AF072709) putative oxidoreductase from Streptomyces lividans (313 aa) fasta scores; opt: 1311, z-score: 1369.8, E(): 0, 66.1% identity in 310 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and Prosite match to PS01162 Quinone oxidoreductase / zeta-crystallin signature. 3 3 5 14 [Reporter]SCO6895 (8C22)_PCR/5141 [Gene]SCO6895/4688 NC_003888 SC1B2.01, possible regulator, partial CDS, len: > 51 aa. Overlaps and extends into CDS SC7F9.47 on the adjoining cosmid which is weakly similar to Streptomyces pilosus TR:Q54804(EMBL:Z50048) iron repressor, DesR.,SC7F9.47, possible regulator, partial CDS, len: >153 aa. Weakly similar to Streptomyces pilosus TR:Q54804(EMBL:Z50048) iron repressor, DesR (231 aa), fasta scores opt: 113 z-score: 145.9 E(): 1 29.4% identity in 143 aa overlap. 3 4 22 13 [Reporter]SCO1924 (10C13)_PCR/7529 [Gene]SCO1924/6860 NC_003888 SCC22.06c, conserved hypothetical protein, len: 394aa; similar to many eg. TR:O53153 (EMBL:AL021184) hypothetical protein from Mycobacterium tuberculosis (397 aa) fasta scores; opt: 1446, z-score: 1649.9, E(): 0, (58.0% identity in 388 aa overlap). Contains Pfam match to entry PF01458 UPF0051, Uncharacterized protein family (UPF0051). 3 3 5 11 [Reporter]SCO4368 (11G18)_PCR/5138 [Gene]SCO4368/4687 NC_003888 SCD19.23c, possible lipase (putative secreted protein), len: 386 aa; similar to TR:Q9Z360 (EMBL:AL035654) Streptomyces coelicolor putative lipase SCE8.12c, 391 aa; fasta scores: opt: 867 z-score: 923.3 E(): 0; 44.3% identity in 393 aa overlap. Contains possible N-terminal region signal peptide sequence 3 3 5 10 [Reporter]SCO4285 (12C18)_PCR/5137 [Gene]SCO4285/4686 NC_003888 SCD95A.18, possible sugar kinase, len: 326 aa; similar to TR:CAB60179 (EMBL:AL132824) Streptomyces coelicolor putative sugar kinase SCAH10.25, 382 aa; fasta scores: opt: 449 z-score: 453.3 E(): 8.7e-18; 37.2% identity in 301 aa overlap. Contains Pfam match to entry PF00480 ROK, ROK family and 5x degenerate repeat: (S/A)AP 3 3 5 9 [Reporter]SCO1192 (13O14)_PCR/5136 [Gene]SCO1192/4685 NC_003888 SCG11A.23, hypothetical protein, len:122 aa; similar to various hypothetical proteins, e.g. TR:O53744 (EMBL:AL021933) Mycobacterium tuberculosis hypothetical 17.2 KD protein MTV038.03, 163 aa; fasta scores: opt: 478 z-score: 600.7 E(): 4.4e-26; 66.7% identity in 111 aa overlap 3 3 5 8 [Reporter]SCO0493 (14K14)_PCR/5135 [Gene]SCO0493/4684 NC_003888 SCF34.12c, probable ABC-transporter transmembrane protein, len: 601 aa; similar to the C-terminal half of TR:O85018 (EMBL:AF034152), ExiT, Mycobacterium smegmatis ABC-transporter associated with ferric exochelin biosynthetic and uptake (possibly involved in exochelin secretion) (1122 aa), fasta scores; opt: 1778 z-score: 1789.6 E(): 0, 50.4% identity in 585 aa overlap, to TR:Q54204 (EMBL:X89010), StrW, Streptomyces glaucescens putative ABC-transporter from the hydroxystreptomycin production locus (591 aa) (33.1% identity in 577 aa overlap) and to many other ABC-transporters. Similar to many others from S.coelicolor e.g. to the C-terminal half of TR:Q9ZBY5 (EMBL:AL034355) CydCD, S.coelicolor probable ABC transporter (1172 aa) (30.3% identity in 574 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam matches to entry PF00005 ABC_tran, ABC transporter and to entry PF00664 ABC_membrane, ABC transporter transmembrane region. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 3 3 5 7 [Reporter]SCO0598 (15G14)_PCR/5134 [Gene]SCO0598/4683 NC_003888 SCF55.22, probable anti anti sigma factor, len: 97 aa; identical to previously sequenced TR:CAB55831 (EMBL:AJ249580) Streptomyces coelicolor putative anti anti sigma factor, 97 aa 3 3 5 6 [Reporter]SCO0537 (16C14)_PCR/5133 [Gene]SCO0537/4682 NC_003888 SCF11.17, hypothetical secreted protein, len: 275 aa; highly similar to TR:P95755 (EMBL:D89734) Streptomyces griseus hypothetical protein adjacent to SgaA, 258 aa; fasta scores: opt: 858 z-score: 936.4 E(): 0; 54.9% identity in 264 aa overlap and to TR:CAB51262 (EMBL:AL096872) Streptomyces coelicolor putative endo alpha-1,4 polygalactosaminidase SC5F7.23c, 282 aa; fasta scores: opt: 303 z-score: 336.5 E(): 2.3e-11; 33.6% identity in 223 aa overlap. Contains probable N-terminal signal sequence 3 3 5 5 [Reporter]SCO0798 (17O10)_PCR/5132 [Gene]SCO0798/4681 NC_003888 SCF43.09, putative secreted protein, len: 411 aa. Weakly similar to several proteins of undefined function e.g. Lactococcus lactis TR:O86271(EMBL:AJ001008) OrfB (341 aa), fasta scores opt: 579 z-score: 550.5 E(): 2.8e-23 31.1% identity in 347 aa overlap. 3 3 5 4 [Reporter]SCO1458 (18K10)_PCR/5131 [Gene]SCO1458/4680 NC_003888 SCL6.15c, probable asnC-family regulatory protein, len: 150 aa; similar to SW:ASNC_HAEIN (EMBL:U32738) Haemophilus influenzae regulatory protein AsnC, 150 aa; fasta scores: opt: 229 z-score: 298.6 E(): 3.3e-09; 28.0% identity in 143 aa overlap. Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family and match ot Prostie entry PS00519 Bacterial regulatory proteins, asnC family signature and possible helix-turn-helix motif at residues 19..40 (+3.26 SD) 2 1 17 6 [Reporter]SCO1781 (17B4)_PCR/861 [Gene]SCO1781/789 NC_003888 SCI51.21c, probable inorganic polyphosphate/ATP-NAD kinase, len: 301 aa; similar to many bacterial hypothetical proteins e.g. TR:O33196 (EMBL:Z98268) Mycobacterium tuberculosis hypothetical protein (307 aa), fasta scores; opt: 975 z-score: 1090.7 E(): 0, 53.6% identity in 302 aa overlap. Highly similar to SW:PPNK_MYCTU (EMBL:AB044336) Mycobacterium tuberculosis inorganic polyphosphate/ATP-NAD kinase (EC 2.7.1.23) (poly(P)/ATP NAD kinase) PpnK, 307 aa; fasta scores: opt: 975 Z-score: 1068.4 E(): 7e-52; 53.642% identity in 302 aa overlap 2 1 17 5 [Reporter]SCO3755 (17N24)_PCR/860 [Gene]SCO3755/788 NC_003888 SCH63.02c, possible ABC transport system ATP binding protein, len: 164 aa; similar to TR:CAB81857 (EMBL:AL161691) Streptomyces coelicolor putative ABC-transporter ATP binding protein SCD40A.12c, 246 aa; fasta scores: opt: 1007 z-score: 1087.6 E(): 0; 67.4% identity in 236 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 2 1 17 3 [Reporter]SCO6298 (19F24)_PCR/858 [Gene]SCO6298/787 NC_003888 SCBAC8D1.11c, conserved hypothetical protein, len: 308 aa; similar to TR:Q9HTH7 (EMBL:AE004951) Pseudomonas aeruginosa hypothetical protein PA5387, 294 aa; fasta scores: opt: 1301 Z-score: 1431.2 bits: 272.9 E(): 3.9e-72; 64.746% identity in 295 aa overlap 2 1 17 2 [Reporter]SCO5077 (20N20)_PCR/857 [Gene]SCO5077/786 NC_003888 SCBAC28G1.03, actVA2, hypothetical protein, len: 131 aa; identical to previously sequenced TR:Q53904 (EMBL:X58833) Streptomyces coelicolor 6 ActVA region genes of the actinorhodin biosynthetic gene cluster, ActVA2, 131 aa 2 1 16 22 [Reporter]SCO1325 (1N16)_PCR/855 [Gene]SCO1325/785 NC_003888 2SCG61.07, conserved hypothetical protein, len: 157 aa; similar to many e.g. SW:O05774 (YU95_MYCTU) hypothetical protein from Mycobacterium tuberculosis (158 aa) fasta scores; opt: 407, z-score: 510.5, E(): 5.9e-21, 43.9% identity in 139 aa overlap and TR:Q9RD98 (EMBL:AL136058) conserved hypothetical protein from Streptomyces coelicolor (184 aa) fasta scores; opt: 337, z-score: 423.9, E(): 3.9e-16, 42.1% identity in 140 aa overlap. Contains Pfam match to entry PF01638 DUF24, Protein of unknown function 2 1 16 21 [Reporter]SCO2230 (2J16)_PCR/854 [Gene]SCO2230/784 NC_003888 SC10B7.25c, malF, putative maltose permease, len: 334 aa; identical to previously sequenced TR:P72398 (EMBL:Y07706) Streptomyces coelicolor putative maltose permease MalF, 334 aa and similar to SW:MALF_ECOLI (EMBL:J01648) Escherichia coli maltose transport system permease protein MalF, 514 aa; fasta scores: opt: 424 z-score: 504.4 E(): 1e-20; 33.9% identity in 239 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophonic membrane spanning regions 2 1 16 20 [Reporter]SCO7632 (3F16)_PCR/853 [Gene]SCO7632/783 NC_003888 SC10F4.05, conserved hypothetical protein, len: 277 aa; similar to TR:O52555 (EMBL:AF040570) Amycolatopsis mediterranei negative regulatory protein RifO, 255 aa; fasta scores: opt: 503 z-score: 563.6 E(): 7.4e-24; 36.4% identity in 272 aa overlap 2 1 16 19 [Reporter]SCO4832 (4B16)_PCR/852 [Gene]SCO4832/782 NC_003888 SC2A6.17, possible glycine betaine-binding lipoprotein, len: 322 aa; similar to SW:PROX_ECOLI (EMBL:M24856) Escherichia coli glycine betaine-binding periplasmic protein precursor ProX or ProU, 330 aa; fasta scores: opt: 112 z-score: 137.8 E(): 3.4; 21.8% identity in 325 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 2 1 16 18 [Reporter]SCO6371 (5N12)_PCR/851 [Gene]SCO6371/781 NC_003888 SC4A2.07, probable integral membrane protein, len: 279 aa; similar to TR:Q98E10 (EMBL:AP003004) Rhizobium loti (Mesorhizobium loti) putative integral membrane protein MLR4456 309 aa; fasta scores: opt: 899 Z-score: 1025.7 E(): 1.7e-49; 56.126% identity in 253 aa overlap 2 1 16 17 [Reporter]SCO6539 (6J12)_PCR/850 [Gene]SCO6539/780 NC_003888 SC5C7.24c, hypothetical protein, len: 272 aa; similar to TR:Q985F1 (EMBL:AP003012) Rhizobium loti (Mesorhizobium loti) MLL7704 protein, 270 aa; fasta scores: opt: 786 Z-score: 877.1 E(): 3.2e-41; 48.638% identity in 257 aa overlap 3 4 22 12 [Reporter]SCO4326 (11O9)_PCR/7528 [Gene]SCO4326/6859 NC_003888 SCD12A.09c, conserved hypothetical protein, len: 282 aa; similar to TR:AAF10587 (EMBL:AE001953) Deinococcus radiodurans conserved hypothetical protein DR1011, 301 aa; fasta scores: opt: 762 z-score: 923.2 E(): 0; 47.6% identity in 275 aa overlap 3 4 22 11 [Reporter]SCO4135 (12K9)_PCR/7527 [Gene]SCO4135/6858 NC_003888 SCD84.02, possible lipoprotein, len: 297 aa; similar to TR:CAB75314 (EMBL:AL139164) Streptomyces coelicolor putative lipoprotein SCC46.10, 285 aa; fasta scores: opt: 180 z-score: 203.0 E(): 0.00075; 22.4% identity in 232 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 3 4 22 10 [Reporter]SCO3244 (13G9)_PCR/7526 [Gene]SCO3244/6857 NC_003888 SCE29.13c, putative secreted protein, len: 265 aa; similar to SW:Y0BO_MYCTU hypothetical protein from Mycobacterium tuberculosis (529 aa) fasta scores; opt: 116, z-score: 133.8, E(): 3.8, (38.2% identity in 76 aa overlap). Contains possible N-terminal region signal peptide sequence 3 4 22 9 [Reporter]SCO0610 (14C9)_PCR/7525 [Gene]SCO0610/6856 NC_003888 SCF55.34, possible gntR-family transcriptional regulator, len: 256 aa; similar to TR:CAB50931 (EMBL:AL096849) Streptomyces coelicolor putative GntR- family transcriptional regulator, 249 aa; fasta scores: opt: 328 z-score: 379.5 E(): 9.2e-14; 31.6% identity in 256 aa overlap and low similarity to SW:FARR_ECOLI (EMBL:X15790) Eschericha coli fatty acyl responsive regulator (P30 PROTEIN) 240 aa; fasta scores: opt: 214 z-score: 251.7 E(): 1.2e-06; 27.5% identity in 211 aa overlap. Contains match to Pfam entry PF00392 gntR, Bacterial regulatory proteins, gntR family 3 4 22 8 [Reporter]SCO1893 (15O5)_PCR/7524 [Gene]SCO1893/6855 NC_003888 SCI7.11c, possible lyase, len: 130aa; similar to many egs. TR:O86701 (EMBL:AL031515) putative lyase from Streptomyces coelicolor (138 aa) fasta scores; opt: 153, z-score: 201.1, E(): 0.00072, (30.5% identity in 131 aa overlap) and SW:LGUL_ECOLI lactolylglutathione lyase from Escherichia coli (135 aa) fasta scores; opt: 111, z-score: 150.2, E(): 0.49, (28.3% identity in 138 aa overlap). 3 4 22 7 [Reporter]SCO0440 (16K5)_PCR/7523 [Gene]SCO0440/6854 NC_003888 SCF51A.18c, possible integral membrane protein, len: 239 aa. Contains multiple possible membrane spanning hydrophobic domains. 3 4 22 6 [Reporter]SCO1191 (17G5)_PCR/7522 [Gene]SCO1191/6853 NC_003888 SCG11A.22c, possible marR-family transcriptional regulator, len: 157 aa; low similarity to TR:CAB48917 (EMBL:AL096837) Streptomyces coelicolor putative marR-family transcriptional regulator SCF43A.30c, 169 aa; fasta scores: opt: 125 z-score: 167.7 E(): 0.058; 30.6% identity in 147 aa overlap 3 4 22 4 [Reporter]SCP1.156 (19O1)_PCR/7520 [Gene]SCP1.156/6852 NC_003888 SCP1.156, unknown, len: 97aa; 3 3 5 3 [Reporter]SCP1.04c (19G10)_PCR/5130 [Gene]SCP1.04c/4679 NC_003888 SCP1.04c, unknown, len: 78aa; 3 4 22 3 [Reporter]SCO4007 (20K1)_PCR/7519 [Gene]SCO4007/6851 NC_003888 2SC10A7.11c, possible integral membrane protein, len: 430 aa; similar to TR:P96675 (EMBL:AB001488) Bacillus subtilis YdeR protein, 395 aa; fasta scores: opt: 611 z-score: 612.1 E(): 1.4e-26; 31.4% identity in 373 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 5 2 [Reporter]SCO1972 (20O6)_PCR/5129 [Gene]SCO1972/4678 NC_003888 SC3C9.07, probable sugar kinase, len: 311 aa; similar to SW:SCRK_KLEPN (EMBL:X61004) Klebsiella pneumoniae fructokinase (EC 2.7.1.4) ScrK, 307 aa; fasta scores: opt: 294 Z-score: 321.0 bits: 67.5 E(): 2.9e-10; 31.541% identity in 279 aa overlap. Contains Pfam match to entry PF00294 pfkB, pfkB family carbohydrate kinase and match to Prosite entry PS00584 pfkB family of carbohydrate kinases signature 2 3 4 21 22 [Reporter]SCO1137 (1C21)_PCR/7516 [Gene]SCO1137/6850 NC_003888 2SCG38.30c, probable two component system histidine kinase, len: 548 aa; similar to SW:DCUS_ECOLI (EMBL:U14003) Escherichia coli sensor protein (EC 2.7.3.-) DcuS, 543 aa; fasta scores: opt: 657 z-score: 720.4 E(): 1.1e-32; 27.0% identity in 529 aa overlap. Contains Pfam match to entry PF00512 signal, Histidine kinase and possible hydrophobic membrane spanning regions 3 3 4 22 [Reporter]SCO4547 (1O2)_PCR/5127 [Gene]SCO4547/4677 NC_003888 2SCD4.18, possible secreted protein, len: 355 aa; similar to TR:CAB77312 (EMBL:AL160312) Streptomyces coelicolor putative secreted protein SCD35.28c, 361 aa; fasta scores: opt: 942 z-score: 949.2 E(): 0; 43.9% identity in 360 aa overlap. Contains possible N-terminal signal peptide sequence 3 3 4 21 [Reporter]SCO5259 (2K2)_PCR/5126 [Gene]SCO5259/4676 NC_003888 2SC7G11.21c, atrB, permease, len: 316 aa; previously sequenced as TR:Q9RGW7 (EMBL:AF104994) Streptomyces coelicolor A3(2) permease AtrB, 313 aa. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 3 3 4 20 [Reporter]SCO2793 (3G2)_PCR/5125 [Gene]SCO2793/4675 NC_003888 2SCC13.01, ornA, oligoribonuclease, len: 200 aa; identical to previously sequenced TR:BAB03461 (EMBL:AB036424) Streptomyces coelicolor oligoribonuclease OrnA, 200 aa. Contains Pfam match to entry PF00929 Exonuclease, Exonuclease 3 3 4 19 [Reporter]SCO6164 (4C2)_PCR/5124 [Gene]SCO6164/4674 NC_003888 SC1A9.28c, unknown, len: 192aa; some similarity to TR:O66611 (EMBL:AE000680) putative protein from Aquifex aeolicus (145 aa) fasta scores; opt: 188, z-score: 294.0, E():4.4e-09, (36.6% identity in 93 aa overlap) 3 3 4 18 [Reporter]SCO2027 (4O22)_PCR/5123 [Gene]SCO2027/4673 NC_003888 SC3A3.05c, possible membrane protein, len: 243 aa; unknown function, shows weak similarity to TR:Q9ZUA5 (EMBL:AC006069) Arabidopsis thaliana hypothetical protein (250 aa), fasta scores; opt: 347 z-score: 387.7 E(): 3e-14, 29.7% identity in 229 aa overlap. Contains hydrophobic, possible membrane-spanning regions 3 3 4 17 [Reporter]SCO7058 (5K22)_PCR/5122 [Gene]SCO7058/4672 NC_003888 SC4G1.24c, possible integral membrane protein, len: 194 aa; similar to TR:Q9RIY8 (EMBL:AL109962) Streptomyces coelicolor hypothetical 20.8 kD protein SCJ1.26, 193 aa; fasta scores: opt: 412 z-score: 478.0 E(): 3.7e-19; 38.8% identity in 183 aa overlap. Contains possible hydrophobic membrane spanning regions 2 2 11 6 [Reporter]SCO3319 (16J19)_PCR/2745 [Gene]SCO3319/2499 NC_003888 SCE68.17c, probable glutamyl-tRNA reductase, len: 581 aa; similar to many e.g. SW:HEM1_BACSU (EMBL:M57676), HemA, Bacillus subtilis glutamyl-tRNA reductase (455 aa), fasta scores; opt: 558 z-score: 588.2 E(): 1.9e-25, 36.3% identity in 300 aa overlap. Contains Pfam match to entry PF00745 GlutR, Glutaminyl-tRNA reductase and PS00747 Glutamyl-tRNA reductase signature 3 3 4 16 [Reporter]SCO6719 (6G22)_PCR/5121 [Gene]SCO6719/4671 NC_003888 SC5F2A.02c, UvrA-like ABC transporter, len: 752 aa; similar to many egs. TR:O86699 (EMBL:AL031515) UvrA-like protein from Streptomyces coelicolor (796 aa) fasta scores; opt: 2047, z-score: 2164.0, E(): 0, (51.9% identity in 776 aa overlap) and SW:UVRA_ECOLI UvrA, excinuclease ABC subunit A from Escherichia coli (940 aa) fasta scores; opt: 634, z-score: 669.2, E(): 5.8e-30, (33.9% identity in 938 aa overlap). Contains two Pfam matches to entry PF00005 ABC_tran, ABC transporter and two Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop). Also contains possible membrane spanning hydrophobic regions. 2 2 11 5 [Reporter]SCO0405 (17F19)_PCR/2744 [Gene]SCO0405/2498 NC_003888 SCF51.04, conserved hypothetical protein, len: 272 aa; similar to TR:O86540 (EMBL:AL031350) Streptomyces coelicolor hypothetical 32.8 kD protein, 300 aa; fasta scores: opt: 656 z-score: 793.0 E(): 0; 43.1% identity in 246 aa overlap 3 3 4 15 [Reporter]SCO7427 (7C22)_PCR/5120 [Gene]SCO7427/4670 NC_003888 SC6D11.23, unknown, len: 148 aa. Similar to several proteins of undefined function e.g. Vibrio parahaemolyticus SW:YJEB_VIBPA(EMBL:U09005) hypothetical 15.6 KD protein (141 aa), fasta scores opt: 292 z-score: 356.7 E(): 1.9e-12 39.7% identity in 146 aa overlap. 2 1 16 16 [Reporter]SCO3591 (7F12)_PCR/849 [Gene]SCO3591/779 NC_003888 SC66T3.02c, putative small membrane protein, len: 41 aa; unknown function, improbable CDS suggested by GC frame plot, amino acid composition and possible RBS. Contains possible hydrophobic membrane spanning region 2 2 11 4 [Reporter]SCO3819 (18B19)_PCR/2743 [Gene]SCO3819/2497 NC_003888 SCGD3.20, hypothetical protein, len: 135 aa; unknown function, probable CDS suggested by probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 2 1 16 15 [Reporter]SCO5294 (8B12)_PCR/848 [Gene]SCO5294/778 NC_003888 SC6G9.39, probable secreted protein, len: 391 aa; similar to mycobacterial hypothetical proteins e.g. TR:O69580 (EMBL:AL022602) Mycobacterium leprae probable secreted protein (374 aa), fasta scores; opt: 347 z-score: 354.0 E(): 2.2e-12, 29.1% identity in 337 aa overlap. Similar to part of Listeria p60 attachment/invasion proteins e.g. SW:P60_LISWE (EMBL:M80354) Listeria welshimeri protein p60 precursor (524 aa) (37.8% identity in 98 aa overlap). Similar to other putative secreted proteins from S.coelicolor e.g. (EMBL:AL049497), SC6G10.08C, S.coelicolor putative secreted protein (338 aa) (38.3% identity in 342 aa overlap). Contains possible N-terminal signal sequence. The C-terminus overlaps the C-terminus of the downstream ORF on the opposite strand. Contains probable coiled-coils from 43 to 98 (56 residues) ( Max score: 1.580, probability 0.99) and from 164 to 201 (38 residues) (Max score: 1.531, probability 0.97). Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family (domain of unknown function) 2 2 11 3 [Reporter]SCP1.294 (19N15)_PCR/2742 [Gene]SCP1.294/2496 NC_003888 SCP1.294, unknown, len: 145aa; similar to regions of others of undefined function eg. TR:Q9ZBJ0 (EMBL:AL035161) conserved hypothetical protein from Streptomyces coelicolor (860 aa) fasta scores; opt: 272, z-score: 347.5, E(): 6.9e-12, 39.4% identity in 127 aa overlap. 2 1 16 14 [Reporter]SCO6986 (9N8)_PCR/847 [Gene]SCO6986/777 NC_003888 SC8F11.12, possible DNA-binding protein, len: 348 aa. Weakly similar to a hypothetical protein located on the Agrobacterium tumefaciens Ti-plasmid TR:BAA87720 (EMBL:AB016260) TiORF95 (336 aa), fasta scores opt: 464 z-score: 513.2 E(): 3.7e-21 29.8% identity in 329 aa overlap and to TR:CAC46423 (EMBL:AL591788) Rhizobium meliloti hypothetical transcription regulator protein SMC00163, 340 aa; fasta scores: opt: 1133 Z-score: 1207.2 E(): 1.3e-59; 52.959% identity in 338 aa overlap. Contains a putative helix-turn-helix motif situated between residues 5..26 (+5.89 SD) 2 2 11 2 [Reporter]SCO3059 (20F15)_PCR/2741 [Gene]SCO3059/2495 NC_003888 SCBAC19G2.14c, phosphoribosylaminoimidazole carboxylase catalytic subunit PurE, len: 180aa: strongly similar to many eg. SW:Q44679 (PUR6_CORAM) phosphoribosylaminoimidazole carboxylase catalytic subunit PurE from Corynebacterium ammoniagenes (177 aa) fasta scores; opt: 666, Z-score: 735.8, 65.541% identity (65.541% ungapped) in 148 aa overlap and TR:O80937 (EMBL:AC004684) putative phosphoribosylaminoimidazole carboxylase from Arabidopsis thaliana (645 aa) fasta scores; opt: 644, Z-score: 703.2, 60.870% identity (60.870% ungapped) in 161 aa overlap. Contains Pfam match to entry PF00731 AIRC, AIR carboxylase. 2 1 16 13 [Reporter]SCO2515 (10J8)_PCR/846 [Gene]SCO2515/776 NC_003888 SCC121.18, possible lipoprotein, len: 179 aa; similar to TR:O86780 (EMBL:AL031317) Streptomyces coelicolor putative lipoprotein SC6Ga.17, 268 aa; fasta scores: opt: 145 z-score: 162.0 E(): 0.12; 32.1% identity in 134 aa overlap. Contains match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide sequence 2 2 10 22 [Reporter]SCO2800 (1F11)_PCR/2739 [Gene]SCO2800/2494 NC_003888 2SCC13.08c, probable two component system histidine kinase, len: 470 aa; similar to many other Streptomyces coelicolor two component system histidine kinases, e.g. TR:Q9RK59 (EMBL:AL132662) putative histidine kinase protein SCF11.31c, 442 aa; fasta scores: opt: 1185 z-score: 1227.7 E(): 0; 47.1% identity in 448 aa overlap. Contains Pfam matches to entries PF00672 DUF5, HAMP domain and PF00512 signal, Histidine kinase. Contains possible hydrophobic membrane spanning regions 2 1 16 12 [Reporter]SCO2548 (11F8)_PCR/845 [Gene]SCO2548/775 NC_003888 SCC77.15c, possible Hit-family protein, len: 117 aa. Similar to many proteins carrying the HIT (histidine triad) family motif which is thought to mediate nucleotide binding, including: Oryctolagus cuniculus (Rabbit) SW:IPK1_RABIT(EMBL:Y11175) HinT protein (protein kinase C inhibitor 1) (125 aa), fasta scores opt: 338 z-score: 429.0 E(): 1.7e-16 47.4% identity in 114 aa overlap and Aquifex aeolicus SW:YHIT_AQUAE(EMBL:AE000675) hypothetical Hit-like protein AQ_141 (121 aa), fasta scores opt: 369 z-score: 467.0 E(): 1.3e-18 46.2% identity in 119 aa overlap. Contains a Pfam match to entry PF01230 HIT. 2 2 10 21 [Reporter]SCO7036 (2B11)_PCR/2738 [Gene]SCO7036/2493 NC_003888 SC4G1.02, argG, argininosuccinate synthase, len: 481 aa; identical to previously sequenced SW:ASSY_STRCO (EMBL:D00799) Streptomyces coelicolor argininosuccinate synthase (EC 6.3.4.5) ArgG, 480 aa. Contains Pfam match to entry PF00764 Arginosuc_synth, Arginosuccinate synthase and matches to Prosite entries PS00564 Argininosuccinate synthase signature 1 and PS00565 Argininosuccinate synthase signature 2 2 1 16 11 [Reporter]SCO4465 (12B8)_PCR/844 [Gene]SCO4465/774 NC_003888 SCD65.08c, hypothetical protein, len: 176 aa; similar to TR:P71704 (EMBL:Z80775) Mycobacterium tuberculosis hypothetical 20.4 kDa protein precursor MTCY21D4.10c, 180 aa; fasta scores: opt: 278 z-score: 350.1 E(): 5.3e-12; 33.7% identity in 172 aa overlap 2 2 10 20 [Reporter]SCO5591 (3N7)_PCR/2737 [Gene]SCO5591/2492 NC_003888 SC2E1.08, rpsP, 30S ribosomal protein S16, highly similar to many e.g. RS16_MYCTU (162 aa), fatsa scores; opt:480 z-score: 771.3 E(): 0, 56.3% identity in 144 aa overlap. Contains PS00732 Ribosomal protein S16 signature 2 1 16 10 [Reporter]SCO3441 (13N4)_PCR/843 [Gene]SCO3441/773 NC_003888 SCE36.08, putative secreted protein, len: 189 aa. Contains possible N-terminal region signal peptide sequence 2 2 10 19 [Reporter]SCO2244 (4J7)_PCR/2736 [Gene]SCO2244/2491 NC_003888 SC1G2.06c, probable serine/threonine protein kinase, len: 686 aa. Highly similar to many serine/threonine protein kinases including: Streptomyces coelicolor SW:AFSK_STRCO (EMBL:D15062) AfsK (799 aa), fasta scores opt: 818 z-score: 736.0 E():0 34.2% identity in 658 aa overlap and Thermomonospora curvata SW:PKWA_THECU (EMBL:U23820) PkwA (742 aa), fasta scores opt: 1065 z-score: 956.9 E():0 37.0% identity in 763 aa overlap. Contains a Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and 3xPfam matches to entry PF00400 WD40, WD domain, G-beta repeat. Also contains Prosite hits to PS00017 ATP/GTP-binding site motif A (P-loop), PS00678 Trp-Asp (WD) repeats signature, PS00108 Serine/Threonine protein kinases active-site signature and PS00107 Protein kinases ATP-binding region signature. 2 1 16 9 [Reporter]SCO2865 (14J4)_PCR/842 [Gene]SCO2865/772 NC_003888 SCE6.02, possible regulatory protein, len: 280 aa. Similar to several proteins of undefined function including: Streptomyces albulus TR:BAA89264 (EMBL:AB025200) hypothetical protein ORF312 (285 aa), fasta scores opt: 585 z-score: 728.2 E():0 38.2% identity in 280 aa overlap and Streptomyces coelicolor TR:Q9Z543 (EMBL:AL035212) putative DNA binding protein, SC9B2.16C (303 aa), fasta scores opt: 313 z-score: 391.9 E(): 2.1e-14 31.0% identity in 284 aa overlap. Also weakly similar to SCE6.06, also located on this cosmid, fasta scores opt: 269 z-score: 293.0 E(): 8.1e-11 27.5% identity in 265 aa overlap. Contains a Pfam match to entry PF01381 HTH_3, Helix-turn-helix with the putative helix-turn-helix motif situated between residues 25..46 (+5.35 SD). 2 2 10 18 [Reporter]SCO5733 (5F7)_PCR/2735 [Gene]SCO5733/2490 NC_003888 SC3C3.19c, unknown, len: 162 aa 2 1 16 8 [Reporter]SCO0679 (15F4)_PCR/841 [Gene]SCO0679/771 NC_003888 SCF91.39, unknown, len: 71 aa. 2 1 16 7 [Reporter]SCO3729 (16B4)_PCR/840 [Gene]SCO3729/770 NC_003888 SCH22A.07c, possible regulatory protein, len: 260 aa; similar to SW:BRPA_STRHY (EMBL:M64783) Streptomyces hygroscopicus bialaphos biosynthetic pathway regulatory protein BrpA, 256 aa; fasta scores: opt: 267 z-score: 309.8 E(): 7.8e-10; 33.8% identity in 160 aa overlap. Contains possible helix-turn-helix motif at residues 214..235 (+3.28 SD) 3 4 21 20 [Reporter]SCO6413 (3K17)_PCR/7514 [Gene]SCO6413/6849 NC_003888 SC1A6.02, possible DNA-binding protein, len: 537 aa; some similarity to a regulator of polyketide gene expression from Streptomyces ambofaciens; TR:Q00509 (EMBL:X63451) srmR (604 aa), fasta scores; opt: 160 z-score: 215.6 E(): 7e-05, 26.8% identity in 400 aa overlap. Contains probable helix-turn-helix motifat aa 486-507 (Score 1520, +4.36 SD) 3 4 21 19 [Reporter]SCO5601 (4G17)_PCR/7513 [Gene]SCO5601/6848 NC_003888 SC2E1.18, unknown, len: 102 aa; highly similar to M. tuberculosis hypothetical protein YX32_MYCTU MTCY274.32c (101 aa), fasta scores; opt: 515 z-score: 1085.2 E(): 0, 75.0% identity in 100 aa overlap 3 4 21 17 [Reporter]SCO6744 (6O13)_PCR/7511 [Gene]SCO6744/6847 NC_003888 SC5F2A.27, unknown, len: 67 aa 3 4 21 15 [Reporter]SCO5211 (8G13)_PCR/7509 [Gene]SCO5211/6846 NC_003888 SC7E4.08c, conserved hypothetical protein, len: 337 aa; similar to TR:O05873 (EMBL:Z95121) Mycobacterium tuberculosis hypothetical 34.9 kD protein MTCY20B11.03, 330 aa; fasta scores: opt: 1051 z-score: 1176.2 E(): 0; 52.7% identity in 338 aa overlap. Contains match to Prosite entryPS00017 ATP/GTP-binding site motif A (P-loop) 3 4 21 14 [Reporter]SCO5413 (9C13)_PCR/7508 [Gene]SCO5413/6845 NC_003888 SC8F4.17, possible transcriptional regulator, len: 169 aa; similar to many proposed transcriptional regulators e.g. TR:CAB62730 (EMBL:AL133423) putative MarR-family transcriptional regulator from Streptomyces coelicolor (151 aa) fasta scores; opt: 184, z-score: 242.7, E(): 4.1e-06, 35.1% identity in 111 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family. 3 4 21 13 [Reporter]SCO2564 (10O9)_PCR/7507 [Gene]SCO2564/6844 NC_003888 SCC123.02c, possible DNA-binding protein, len: 336 aa. Highly similar to several proteins of undefined function e.g. Mycobacterium tuberculosis TR:P71730 (EMBL:Z81368) hypothetical 33.1 KD protein (316 aa), fasta scores opt: 792 z-score: 880.3 E():0 42.4% identity in 316 aa overlap. Note that codon 21 may be an alternative translational start site. Contains a possible helix-turn-helix motif situated between residues 263..284 (+2.96 SD) 3 4 21 12 [Reporter]SCO4321 (11K9)_PCR/7506 [Gene]SCO4321/6843 NC_003888 SCD12A.04c, hypothetical protein, len: 867 aa; similar to TR:O53874 (EMBL:AL022004) Mycobacterium tuberculosis hypothetical 79.7 kD protein (fragment) MTV043.55c aa; fasta scores: opt: 651 z-score: 618.2 E(): 5.7e-27; 36.1% identity in 886 aa overlap 3 4 21 11 [Reporter]SCO4479 (12G9)_PCR/7505 [Gene]SCO4479/6842 NC_003888 SCD65.22c, possible secreted hydrolase, len: 297 aa; similar to TR:O31386 (EMBL:Y10223) Bradyrhizobium japonicum b-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) PcaD, 260 aa; fasta scores: opt: 188 z-score: 220.7 E(): 8e-05; 32.1% identity in 156 aa overlap. Contains match to Prosite entry PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Contains possible N-terminal region signal peptide sequence 3 3 4 14 [Reporter]SCO7595 (8O18)_PCR/5119 [Gene]SCO7595/4669 NC_003888 SC7H9.07c, conserved hypothetical protein, len: 391 aa; similar to SW:YDHH_ECOLI (EMBL:AE000259) Escherichia coli hypothetical 39.5 kDa protein in PdxH-SlyB intergenic region YdhH, 369 aa; fasta scores: opt: 851 z-score: 883.8 E(): 0; 42.5% identity in 369 aa overlap 3 4 21 10 [Reporter]SCO3240 (13C9)_PCR/7504 [Gene]SCO3240/6841 NC_003888 SCE29.09c, unknown, len: 236 aa; similar to TR:O69951 (EMBL:AL023862) hypothetical protein from Streptomyces coelicolor (245 aa) fasta scores; opt: 416, z-score: 464.9, E(): 1.4e-18, (45.5% identity in 220 aa overlap). 3 3 4 13 [Reporter]SCO6438 (9K18)_PCR/5118 [Gene]SCO6438/4668 NC_003888 SC9B5.05, dcdA, diaminopimelate decarboxylase, len: 440 aa; similar to many e.g. SW:DCDA_ECOLI DcdA, diaminopimelate decarboxylase from Escherichia coli (420 aa) fasta scores; opt: 1769, z-score: 2033.9, E(): 0, (65.6% identity in 398 aa overlap). Contains PS00878 Orn /DAP /Arg decarboxylases family 2 pyridoxal-P attachment site and PS00879 Orn /DAP /Arg decarboxylases family 2 signature 2. Also contains Pfam match to entry PF00278 Orn_DAP_Arg_deC, Pyridoxal-dependent decarboxylase. 3 4 21 9 [Reporter]SCO2883 (14O5)_PCR/7503 [Gene]SCO2883/6840 NC_003888 SCE6.20, possible cytochrome P450, len: 410 aa. Highly similar to many other cytochrome P450 proteins from Streptomyces including: S. coelicolor TR:CAB61278 (EMBL:AL132991) SCF55.08C (420 aa), fasta scores opt: 710 z-score: 807.4 E():0 33.1% identity in 411 aa overlap and S. violaceus (S. venezuelae) TR:O87605 (EMBL:AF087022) cytochrome P450 monooxygenase, PikC (416 aa), fasta scores opt: 464 z-score: 529.1 E(): 4.8e-22 30.2% identity in 411 aa overlap. Also similar to the adjoining CDS SCE6.21 (408 aa), fasta scores opt: 257 z-score: 252.4 E(): 1.5e-08 26.5% identity in 407 aa overlap. Contains a Prosite hit to PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 3 3 4 12 [Reporter]SCO2460 (10G18)_PCR/5117 [Gene]SCO2460/4667 NC_003888 SCC24.31c, hypothetical protein, len: 142 aa; similar to TR:O06633 (EMBL:Z95618) Mycobacterium tuberculosis hypothetical 12.5 kD protein MTCY07H7A.08c, 115 aa; fasta scores: opt: 109 z-score: 150.6 E(): 0.59; 35.0% identity in 140 aa overlap 3 3 4 11 [Reporter]SCO4364 (11C18)_PCR/5116 [Gene]SCO4364/4666 NC_003888 SCD19.19, unknown, len: 125 aa 3 3 4 10 [Reporter]SCO3291 (12O14)_PCR/5115 [Gene]SCO3291/4665 NC_003888 SCE15.08, possible regulatory protein, len: 477 aa; N-terminal region similar to others from Actinomycetes eg. SW:DNRI_STRPE regulatory protein from Streptomyces peucetius (272 aa) fasta scores; opt: 143, z-score: 162.9, E(): 0.092, (27.8% identity in 241 aa overlap). 3 3 4 9 [Reporter]SCO3209 (13K14)_PCR/5114 [Gene]SCO3209/4664 NC_003888 SCE8.02, possible transcriptional regulator, len: 524 aa; N-terminal (up to approx. 270aa) similar to one group of transcriptional regulator (eg. SW:ICLR_ECOLI acetate operon repressor from Escherichia coli (274 aa) fasta scores; opt: 208, z-score: 221.4, E(): 5e-05, (27.1% identity in 262 aa overlap)) and C-terminal (from approx. 270aa) similar to a different group of transcriptional regulator (eg. SW:MHPR_ECOLI mhp operon transcriptional activator (315 aa) fasta scores; opt: 205, z-score: 217.5, E(): 8.3e-05, (28.5% identity in 267 aa overlap). Both halves appear to be members of the same family of transcriptional regulators (IclR-type) suggesting a duplication event. Contains two probable helix-turn-helix motifs at 37-58aa (Score 1082 (+2.87 SD)) and 320-341aa (Score 1437 (+4.08 SD)). 3 3 4 7 [Reporter]SCO0950 (15C14)_PCR/5112 [Gene]SCO0950/4663 NC_003888 SCM11.05c, probable transport system permease protein, len: 306 aa; similar to TR:P73854 (EMBL:D90910) Synechocystis sp. lactose transport system permease protein LacG, 270 aa; fasta scores: opt: 688 z-score: 826.9 E(): 0; 39.2% identity in 273 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 3 3 4 6 [Reporter]SCO0472 (16O10)_PCR/5111 [Gene]SCO0472/4662 NC_003888 SCF76.12c, possible secreted protein, len: 384 aa. Contains a possible N-terminal signal sequence. 2 2 10 17 [Reporter]SCO2049 (6B7)_PCR/2734 [Gene]SCO2049/2489 NC_003888 SC4G6.18c, conserved hypothetical protein, len: 134aa; previously sequenced therefore almost identical to SW:YHI2_STRCO. Also similar to many others eg. TR:O07205 (EMBL:Z96072) hypothetical protein from Mycobacterium tuberculosis (142 aa) fasta scores; opt: 225, z-score: 300.2, E(): 2.1e-09, (34.1% identity in 123 aa overlap). 3 3 4 5 [Reporter]SCO0705 (17K10)_PCR/5110 [Gene]SCO0705/4661 NC_003888 SCF42.15c, unknown, len: 272 aa. Similar to several proteins of undefined function from Streptomyces coelicolor TR:O86540(EMBL:AL031350) SC1F2.03C (300 aa), fasta scores opt: 639 z-score: 770.1 E():0 41.4% identity in 261 aa overlap and TR:CAB59702(EMBL:AL132707) probable mannanase SCF51.04C (272 aa), fasta scores opt: 744 z-score: 896.2 E():0 49.6% identity in 248 aa overlap 2 2 10 16 [Reporter]SCO3615 (7N3)_PCR/2733 [Gene]SCO3615/2488 NC_003888 SC66T3.26c, ask, probable aspartokinase, len: 425 aa; highly similar to many e.g. SW:AKAB_MYCSM (EMBL:Z17372), ask, Mycobacterium smegmatis aspartokinase (421 aa), fasta scores; opt: 1839 z-score: 1990.8 E(): 0, 67.4% identity in 427 aa overlap. Homologous genes encode both the alpha and beta subunits of ask by using alternative initiation codons. Codon 253 represents the equivalent position in this sequence, by similarity. Contains Pfam match to entry PF00696 aakinase, Aspartate kinases, Glutamate kinases and Gamma glutamate phospho-reductases and PS00324 Aspartokinase signature 3 3 4 4 [Reporter]SCO1694 (18G10)_PCR/5109 [Gene]SCO1694/4660 NC_003888 SCI30A.15c, possible penicillin acylase, len: 893 aa; similar to many eg. SW:PAC_ARTVI penicillin acylase from Arthrobacter viscosus (ATCC 15294) (802 aa) fasta scores; opt: 379, z-score: 409.2, E(): 1.8e-15, (25.5% identity in 733 aa overlap). 2 1 16 6 [Reporter]SCO0557 (16N24)_PCR/839 [Gene]SCO0557/769 NC_003888 SCF73.04, probable transcriptional regulator, len: 340 aa; similar in N-terminus to members of the MerR family e.g. NOLA_BRASN nodulation protein NOLA (237 aa), fasta scores; opt: 211 z-score: 233.9 E(): 1.2e-05, 48.5% identity in 68 aa overlap. Also similar to SCD17.06c (34.8% identity in 322 aa overlap). Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family 2 2 10 14 [Reporter]SCO5668 (9F3)_PCR/2731 [Gene]SCO5668/2487 NC_003888 SC8B7.10c, probable polyamine ABC-transporter ATP-bi nding protein, len: 397 aa; similar to many e.g. POTA_ECOLI spermidine/putrescine transport ATP-binding (378 aa), fast a scores; opt: 933 z-score: 1101.5 E(): 0, 47.7% identity i n 325 aa overlap. Contains PS00017 ATP/GTP-binding site mot if A (P-loop), PS00211 ABC transporters family signature an d Pfam match to entry PF00005 ABC_tran, ABC transporters, s core 216.70, E-value 3.6e-61 2 1 16 5 [Reporter]SCO3770 (17J24)_PCR/838 [Gene]SCO3770/768 NC_003888 SCH63.17, probable cytochrome P450 oxidoreductase, len: 394 aa; similar to SW:CPXK_SACER (EMBL:M83110) Saccharopolyspora erythraea cytochrome P450 107B1 (EC 1.14.-.-) (P450CVIIB1) Cyp107B1, 405 aa; fasta scores: opt: 1038 z-score: 1154.8 E(): 0; 46.9% identity in 373 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450 and match to Prosite entry PS00211 ABC transporters family signature and PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Also contains a TTA leucine codon, possible target for bldA regulation 2 2 10 13 [Reporter]SCO6491 (10B3)_PCR/2730 [Gene]SCO6491/2486 NC_003888 SC9C7.27c, unknown, len: 130aa 2 1 16 4 [Reporter]SCO4973 (18F24)_PCR/837 [Gene]SCO4973/767 NC_003888 2SCK31.33, conserved hypothetical protein, len: 273 aa; similar to TR:Q9RYX5 (EMBL:AE001862) Deinococcus radiodurans conserved hypothetical protein DRA0179, 274 aa; fasta scores: opt: 888 z-score: 1076.8 E(): 0; 52.4% identity in 271 aa overlap 2 2 10 12 [Reporter]SCO2685 (10N23)_PCR/2729 [Gene]SCO2685/2485 NC_003888 SCC61A.06c, possible ATP-binding protein, len: 535aa; no significant database similarities. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). Note possible alternative downstream translational start sites. 2 1 16 3 [Reporter]SCO1302 (19B24)_PCR/836 [Gene]SCO1302/766 NC_003888 SCBAC36F5.13c, conserved hypothetical protein, len: 208 aa; similar to TR:Q9K6U5 (EMBL:AP001519) Bacillus halodurans BH3630 protein, 213 aa; fasta scores: opt: 659 Z-score: 773.5 bits: 150.1 E(): 1.8e-35; 46.341% identity in 205 aa overlap. Contains Pfam match to entry PF01205 UPF0029, Uncharacterized protein family UPF0029 and match to Prosite entry PS00910 Uncharacterized protein family UPF0029 signature 2 2 10 11 [Reporter]SCO4430 (11J23)_PCR/2728 [Gene]SCO4430/2484 NC_003888 SCD6.08, possible secreted protein, len: 189 aa; similar to TR:O86790 (EMBL:AL031317) Streptomyces coelicolor putative secreted protein SC6G4.27c, 184 aa; fasta scores: opt: 495 z-score: 536.2 E(): 2.1e-22; 48.1% identity in 181 aa overlap. Contains possible N-terminal region signal peptide sequence 2 1 16 2 [Reporter]SCO5109 (20J20)_PCR/835 [Gene]SCO5109/765 NC_003888 SCBAC31E11.05c, unknown, len: 146 aa. Contains a possible coiled-coil region at approx. residues 60..87 2 2 10 10 [Reporter]SCO3500 (12F23)_PCR/2727 [Gene]SCO3500/2483 NC_003888 SCE134.01c, partial CDS, unknown, len: >671aa;,SCE65.36c, unknown (partial CDS), len: >41 aa; identical to previously sequenced TR:CAB41199 (EMBL:AL049661) Streptomyces coelicolor SCE134.01c 2 1 15 22 [Reporter]SCO1320 (1J16)_PCR/833 [Gene]SCO1320/764 NC_003888 2SCG61.02c, unknown, len: 281 aa; contains region of alignment with TR:CAB84350 (EMBL:AL162755) putative transferase from Neisseria meningitidis (263 aa) fasta scores; opt: 184, z-score: 212.1, E(): 0.00025, 27.7% identity in 148 aa overlap 2 2 10 9 [Reporter]SCO0952 (13B23)_PCR/2726 [Gene]SCO0952/2482 NC_003888 SCM11.07c, possible solute-binding protein, len: 430 aa; similar to TR:P73325 (EMBL:D90905) Synechocystis sp SrrA, 433 aa; fasta scores: opt: 611 z-score: 678.3 E(): 2.1e-30; 29.8% identity in 430 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and possible N-terminal region signal peptide sequence 2 1 15 21 [Reporter]SCO4740 (2F16)_PCR/832 [Gene]SCO4740/763 NC_003888 SC6G4.18, glmS1, probable glucosamine--fructose-6-phosphate aminotransferase, len: 615 aa; similar to many e.g. GLMS_ECOLI glucosamine--fructose-6-phosphate aminotransferase (isomerizing) (EC 2.6.1.16) (608 aa), fasta scores; opt : 1658 z-score: 1783.2 E(): 0, 44.3% identity in 621 aa ove rlap. Contains Pfam match to entry PF00310 GATase_2, Glutamine amidotransferases class-II, score 226.20, E-value 1.8e-76 2 2 10 8 [Reporter]SCO1614 (14N19)_PCR/2725 [Gene]SCO1614/2481 NC_003888 SCI35.36, probable transcriptional regulator, len: 236 aa; similar to many members of the gntR family e.g. GLCC_ECOLI Glc operon transcriptional activator (254 aa), fasta scores; opt: 183 z-score: 269.1 E(): 9.7e-08, 28.3% identity in 219 aa overlap. Contains PS00043 Bacterial regulatory proteins, gntR family signature, probable helix-turn-helix motif at aa 42-63 (Score 1486, +4.25 SD), and Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family, score 64.70, E-value 2e-18 2 1 15 20 [Reporter]SCO7801 (3B16)_PCR/831 [Gene]SCO7801/762 NC_003888 SC10B8A.11, possible membrane protein, len: 126aa; contains possible membrane-spanning hydrophobic regions. 2 2 10 7 [Reporter]SCO3475 (15J19)_PCR/2724 [Gene]SCO3475/2480 NC_003888 SCE65.11c, possible isomerase, len: 377 aa; similar to SW:ATB_RHOOP (EMBL:X99622) Rhodococcus opacus muconate cycloisomerase I (EC 5.5.1.1) CatB, 373 aa; fasta scores: opt: 390 z-score: 443.3 E(): 2.6e-17; 31.1% identity in 357 aa overlap. Contains Pfam match to entry PF01188 MR_MLE, Mandelate racemase / muconate lactonizing enzyme family PS00908 Mandelate racemase / muconate lactonizing enzyme family signature 1 PS00909 Mandelate racemase / muconate lactonizing enzyme family signature 2 2 1 15 19 [Reporter]SCO7032 (4N12)_PCR/830 [Gene]SCO7032/761 NC_003888 SC1H10.21, unknown, len: 213 aa. Similar to a protein of undefined function from Streptomyces coelicolor TR:Q9XBC6(EMBL:AL078635) hypothetical 22.9 KD protein (205 aa), fasta scores opt: 950 z-score: 1149.9 E(): 0 71.6% identity in 201 aa overlap. 2 1 15 18 [Reporter]SCO6367 (5J12)_PCR/829 [Gene]SCO6367/760 NC_003888 SC4A2.03c, probable ABC transporter ATP-binding protein, len: 245 aa; similar to many e.g. YF08_METJA hypothetical ABC transporter ATP-binding protein (224 aa), fasta scores; opt: 522 z-score: 604.9 E(): 1.9e-26, 40.6% identity in 219 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature and Pfam match to entry PF00005 ABC_tran, ABC transporters, score 175.60, E-value 8.3e-49 3 4 21 8 [Reporter]SCO5019 (15K5)_PCR/7502 [Gene]SCO5019/6839 NC_003888 SCK15.21c, possible secreted protein, len: 201 aa; similar to TR:Q9UZM5 (EMBL:AJ248286;) Pyrococcus abyssi hypothetical 13.4 kD protein, 117 aa; fasta scores: opt: 167 z-score: 206.8 E(): 0.00048; 31.0% identity in 100 aa overlap. Contains possible N-terminal region signal peptide sequence 3 4 21 7 [Reporter]SCO0117 (16G5)_PCR/7501 [Gene]SCO0117/6838 NC_003888 SCJ11.46, possible oxidoreductase, len: 230 aa; similar to many proposed oxidoreductases eg. TR:O53324 (EMBL:AL021646) oxidoreductase from Mycobacterium tuberculosis (235 aa) fasta scores; opt: 626, z-score: 717.5, E(): 1.3e-32, (50.9% identity in 224 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature 3 4 21 6 [Reporter]SCO1743 (17C5)_PCR/7500 [Gene]SCO1743/6837 NC_003888 SCI11.32, possible ABC-transporter transmembrane protein, len: 239 aa; weakly similar to putative ABC-transporter protein from S.coelicolor TR:O69995 (EMBL:AL022374) S.coelicolor probable ABC transporter (744 aa), fasta scores; opt: 197 z-score: 224.6 E(): 3.5e-05, 35.4% identity in 212 aa overlap. Contains hydrophobic, possible membrane-spanning regions. May be associated with the adjacent putative ABC-transporter ATP-binding protein 3 4 21 5 [Reporter]SCO0064 (18O1)_PCR/7499 [Gene]SCO0064/6836 NC_003888 SCJ4.45c, unknown, len: 572 aa 3 4 21 4 [Reporter]SCO6323 (19K1)_PCR/7498 [Gene]SCO6323/6835 NC_003888 SCIF3.25c, probable tetR-family regulatory protein, len: 218 aa; similar to TR:O66121 (EMBL:AB000384) Streptomyces coelicolor regulatory protein CprS, 215 aa; fasta scores: opt: 370 z-score: 471.3 E(): 1e-18; 36.1% identity in 191 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 3 4 21 3 [Reporter]SCP1.162c (20G1)_PCR/7497 [Gene]SCP1.162c/6834 NC_003888 SCP1.162c, possible oxidoreductase subunit, len: 65aa; similar to the extreme C-terminus of many glycerol-3-phosphate dehydrogenases eg. TR:Q9ZBS0 (EMBL:AL034447) glycerol-3-phosphate dehydrogenase from Streptomyces coelicolor (336 aa) fasta scores; opt: 302, z-score: 432.6, E(): 1.3e-16, 76.9% identity in 65 aa overlap. Also similar to neighbouring CDS SCP1.162c fasta scores; opt: 238, z-score: 294.0, E(): 7.6e-12, 50.7% identity in 75 aa overlap. 3 4 21 2 [Reporter]SCO7469 (20O21)_PCR/7496 [Gene]SCO7469/6833 NC_003888 SCBAC17A6.02c, phenylacetate-CoA ligase, len: 448 aa: strongly similar to many e.g. TR:Q9L9C1 (EMBL:AF176259) aerobic phenylacetate-CoA ligase from Azoarcus evansii (440 aa) fasta scores; opt: 1896, Z-score: 2065.6, 63.825% identity (64.871% ungapped) in 434 aa overlap. Contains 2 Pfam matches to entry PF00501 AMP-binding, AMP-binding enzyme. 3 4 20 22 [Reporter]SCO1334 (1O17)_PCR/7494 [Gene]SCO1334/6832 NC_003888 2SCG61.16, unknown, len: 59aa; 3 3 4 3 [Reporter]SCO1301 (19C10)_PCR/5108 [Gene]SCO1301/4659 NC_003888 SCBAC36F5.12c, possible exonuclease, len: 387 aa; similar to SW:SBCD_ECOLI (EMBL:X15981) Escherichia coli exonuclease SbcD or B0398, 400 aa; fasta scores: opt: 394 Z-score: 436.1 bits: 89.5 E(): 1.1e-16; 30.711% identity in 394 aa overlap. Contains Pfam match to entry PF02549 DNA_repair, DNA repair exonuclease 3 4 20 21 [Reporter]SCO4724 (2K17)_PCR/7493 [Gene]SCO4724/6831 NC_003888 SC6G4.02, map, methionine aminopeptidase, len: 278 aa; identical to TR:O54208 (EMBL:X83011) methionine aminopeptidase (278 aa) and highly similar to many e.g.AMPM_BACSU methionine aminopeptidase (EC 3.4.11.18) (248 aa), fasta scores; opt: 546 z-score: 922.2 E(): 0, 44.5% identity in 256 aa overlap. Also similar to S. coelicolor map2 (E(): 3.2e-3 3, 46.8% identity in 267 aa overlap). Contains PS00680 Meth ionine aminopeptidase subfamily 1 signature and Pfam match to entry PF00557 pep_M24, metallopeptidase family M24, scor e 194.80, E-value 1.4e-54 3 3 4 2 [Reporter]SCO4012 (20K6)_PCR/5107 [Gene]SCO4012/4658 NC_003888 2SC10A7.16c, possible integral membrane protein, len: 143 aa; almost identical to TR:Q04914 (EMBL:X70689) Streptomyces lividans ORFC310 protein, 143 aa. Contains possible hydrophobic membrane spanning regions 3 4 20 20 [Reporter]SCO7643 (3G17)_PCR/7492 [Gene]SCO7643/6830 NC_003888 SC10F4.16, unknown, len: 91 aa 3 3 3 22 [Reporter]SCO4542 (1K2)_PCR/5105 [Gene]SCO4542/4657 NC_003888 2SCD4.13c, hypothetical protein, len: 63 aa; identical to previously sequenced TR:Q9XD97 (EMBL:AF106004) Streptomyces coelicolor putative BldB homolog hypothetical 6.8 kDa protein, 63 aa and to TR:Q9RDI7 (EMBL:AL136519) Streptomyces coelicolor hypothetical 7.1 kDa protein SCC57A.10c, 65 aa; fasta scores: opt: 237 z-score: 357.1 E(): 2e-12; 64.8% identity in 54 aa overlap 3 3 3 21 [Reporter]SCO0734 (2G2)_PCR/5104 [Gene]SCO0734/4656 NC_003888 3SC5B7.12c, probable transcriptional repressor, len: 424 aa; similar to SW:MLC_ECOLI (EMBL:D32222) Escherichia coli transcriptional regulator Mlc protein, 406 aa; fasta scores: opt: 387 z-score: 414.7 E(): 1.4e-15; 24.1% identity in 344 aa overlap. Contains Pfam match to entry PF00480 ROK, ROK family and possible helix-turn-helix motif at residues 64..85 (+6.19 SD) 3 3 3 20 [Reporter]SCO2595 (3C2)_PCR/5103 [Gene]SCO2595/4655 NC_003888 SCC88.06c, obg, GTP-binding protein, len: 478 aa; identical to previously sequenced TR:P95722 (EMBL:D87915) Streptomyces coelicolor GTP-binding protein Obg and similar to SW:OBG_BACSU (EMBL:M24537) Bacillus subtilis Spo0B-associated GTP-binding protein Obg, 428 aa; fasta scores: opt: 1164 z-score: 1163.2 E(): 0; 46.2% identity in 416 aa overlap. Contains Pfam match to entry PF01018 GTP1_OBG, GTP1/OBG family and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 3 3 19 [Reporter]SCO1406 (3O22)_PCR/5102 [Gene]SCO1406/4654 NC_003888 SC1A8A.26c, hypothetical protein, len: 328 aa; weakly similar to TR:CAB46782 (EMBL:AL096811) hypothetical protein from Streptomyces coelicolor (338 aa) fasta scores; opt: 140, z-score: 170.3, E(): 0.047, 26.9% identity in 338 aa overlap. 3 3 3 18 [Reporter]SCO6235 (4K22)_PCR/5101 [Gene]SCO6235/4653 NC_003888 SC2H4.17c, unknown, len: 81 aa; similar to S. coelicolor TR:Q53896 (EMBL:X60316) abaA orfD (75 aa), fasta scores; opt: 121 z-score: 174.5 E(): 0.018, 37.7% identity in 53 aa overlap. Also similar to SC4G2.04 (E(): 4.6e-12, 54.5% identity in 55 aa overlap) 3 3 3 17 [Reporter]SCO7131 (5G22)_PCR/5100 [Gene]SCO7131/4652 NC_003888 SC4B10.32, possible lipase, len: 316 aa; similar to TR:O52270 (EMBL:AF034088) Pseudomonas sp. lipase (EC 3.1.1.-) LipP, 308 aa; fasta scores: opt: 675 z-score: 762.7 E(): 0; 38.0% identity in 303 aa overlap 2 2 10 6 [Reporter]SCO0274 (16F19)_PCR/2723 [Gene]SCO0274/2479 NC_003888 SCF85.02, possible alpha-galactosidase, len: 499 aa. Similar to many alpha galactosidases e.g. Thermus aquaticus TR:AAD32628(EMBL; AF135399) alpha-galactosidase (476 aa), fasta scores opt: 1329 z-score: 1530.0 E(): 0 45.8% identity in 487 aa overlap 3 3 3 16 [Reporter]SCO7586 (6C22)_PCR/5099 [Gene]SCO7586/4651 NC_003888 SC5F1.40, probable oxidoreductase, len: 357 aa; similar to SW:NEMA_ECOLI (EMBL:D86931) Escherichia coli N-ethylmaleimide reductase (EC 1.-.-.-) NemA, 365 aa; fasta scores: opt: 830 z-score: 878.8 E(): 0; 40.5% identity in 365 aa overlap. Contains Pfam match to entry PF00724 oxidored_FMN, NADH:flavin oxidoreductase / NADH oxidase family 2 2 10 5 [Reporter]SCO1185 (17B19)_PCR/2722 [Gene]SCO1185/2478 NC_003888 SCG11A.16, hypothetical protein, len: 667 aa; similar to TR:CAB46977 (EMBL:AL096825) Streptomyces coelicolor hypothetical 60.3 KD protein SC6G3.05, 588 aa; fasta scores: opt: 633 z-score: 462.0 E(): 2.3e-18; 40.4% identity in 680 aa overlap 3 3 3 15 [Reporter]SCO4408 (7O18)_PCR/5098 [Gene]SCO4408/4650 NC_003888 SC6F11.06c, unknown, len: 428 aa. Contains 4x degenerate repeat unit: (G/T)GTG 2 1 15 17 [Reporter]SCO5810 (6F12)_PCR/828 [Gene]SCO5810/759 NC_003888 SC4H2.31c, probable transmembrane efflux protein, len: 515 aa; similar to many diverse efflux proteins e.g. CMCT_NOCLA Q04733 cephamycin export protein cmct (486 aa), fasta scores; opt: 1059 z-score: 1207.4 E(): 0, 40.2% identity in 433 aa overlap, and PUR8_STRLP P42670 puromycin resistance protein pur8 (503 aa), fasta scores; opt: 1135 z-score: 1187.0 E(): 0, 39.8% identity in 492 aa overlap. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site and Pfam match to entry sugar_tr PF00083, Sugar (and other) transporters, score 33.27 2 2 10 4 [Reporter]SCO4048 (18N15)_PCR/2721 [Gene]SCO4048/2477 NC_003888 2SCD60.14c, hypothetical protein, len: 241 aa; similar to TR:Q9RL28 (EMBL:AL121596) Streptomyces coelicolor hypothetical 28.7 kDa protein SCF51A.37, 259 aa; fasta scores: opt: 255 z-score: 307.0 E(): 1.5e-09; 30.2% identity in 225 aa overlap 2 1 15 16 [Reporter]SCO5704 (7B12)_PCR/827 [Gene]SCO5704/758 NC_003888 SC5H4.28, probable transcriptional termination/antitermination factor, len: 340 aa; highly similar to TR:Q9Z5J1 (EMBL:AL035472) Mycobacterium leprae putative transcriptional termination/antitermination factor NusA, 347 aa; fasta scores: opt: 1292 z-score: 1449.3 E(): 0; 60.8% identity in 344 aa overlap and similar to SW:NUSA_ECOLI (EMBL:X00513) Escherichia coli N utilization substance protein A (NusA protein) (L factor) NusA, 495 aa; fasta scores: opt: 621 z-score: 698.0 E(): 2e-31; 35.4% identity in 336 aa overlap. Contains Pfam matches to entries PF00575 S1, S1 RNA binding domain and PF00013 KH-domain, KH domain 2 2 10 3 [Reporter]SCP1.242c (19J15)_PCR/2720 [Gene]SCP1.242c/2476 NC_003888 SCP1.242c, mmfR, possible lactone-dependent transcriptional regulator (TetR-family), len: 214aa; previously sequenced and annotated as hypothetical protein TR:Q9JN89 (EMBL:AJ276673). Similar to many lactone-dependent transcriptional regulators eg. TR:Q54189 (EMBL:D49782) A-factor receptor from Streptomyces griseus (276 aa) fasta scores; opt: 342, z-score: 422.0, E(): 4.9e-16, 33.0% identity in 182 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and helix-turn-helix motif at residue 50-71 (Score 1532 (+4.40 SD)). 2 1 15 15 [Reporter]SCO2485 (8N8)_PCR/826 [Gene]SCO2485/757 NC_003888 SC7A8.24c, hypothetical protein, len: 242 aa; similar to various hypothetical proteins, e.g. TR:CAB54589 (EMBL:AJ249205) Vibrio cholerae hypothetical 27.3 kD protein, 261 aa; fasta scores: opt: 509 z-score: 568.8 E(): 2.7e-24; 35.5% identity in 256 aa overlap. Contains possible N-terminal region signal peptide sequence 2 2 10 2 [Reporter]SCO5108 (20B15)_PCR/2719 [Gene]SCO5108/2475 NC_003888 SCBAC31E11.04c, possible integral membrane protein, len: 278 aa; similar to TR:O53671 (EMBL:AL021929) Mycobacterium tuberculosis hypothetical 31.1 kDa protein MTV034.15c, 273 aa; fasta scores: opt: 1010 Z-score: 1230.3 bits: 235.4 E(): 6.5e-61; 54.545% identity in 275 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 15 14 [Reporter]SCO6982 (9J8)_PCR/825 [Gene]SCO6982/756 NC_003888 SC8F11.08, unknown, len:300 aa. Similar to a protein of undefined function involved in rhizopine catabolism in Rhizobium meliloti SW:MOCC_RHIME(EMBL:X78503) MocC (325 aa), fasta scores opt: 390 z-score: 432.2 E(): 1.2e-16 29.8% identity in 289 aa overlap. Also similar to Bacillus subtilis SW:IOLE_BACSU(EMBL:D14399) IolE, found within the inositol utilisation operon (297 aa), fasta scores opt: 313 z-score: 348.9 E(): 5.3e-12 26.3% identity in 293 aa overlap. 2 2 9 22 [Reporter]SCO2796 (1B11)_PCR/2717 [Gene]SCO2796/2474 NC_003888 2SCC13.04, probable cellobiose transport permease, len: 337 aa; highly similar to TR:Q9X9R6 (EMBL:AJ009797) Streptomyces reticuli permease CebF protein, 305 aa; fasta scores: opt: 1416 z-score: 1722.4 E(): 0; 67.9% identity in 296 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 2 1 15 13 [Reporter]SCO7356 (10F8)_PCR/824 [Gene]SCO7356/755 NC_003888 SC9H11.10c, oxidoreductase, len: 276 aa. Highly similar to many including: Corynebacterium sp. SW:2DKG_CORSP(EMBL:M21193) 2,5-diketo-d-gluconic acid reductase (EC 1.1.1.-) (277 aa), fasta scores opt: 1006 z-score: 1188.8 E(): 0 52.9% identity in 274 aa overlap and Streptomyces coelicolor TR:Q9ZBW7(EMBL:AL034443;) putative oxidoreductase, SC4B5.01C (277 aa), fasta scores opt: 1018 z-score: 1202.9 E():0 56.3% identity in 268 aa overlap. Contains Prosite hits to PS00063 Aldo/keto reductase family putative active site signature, PS00062 Aldo/keto reductase family signature 2 and PS00798 Aldo/keto reductase family signature 1. Also contains a Pfam match to entry PF00248 aldo_ket_red, Aldo/keto reductase family. 2 2 9 20 [Reporter]SCO4728 (3J7)_PCR/2715 [Gene]SCO4728/2473 NC_003888 SC6G4.06, rpsK, 30S ribosomal protein S11, len: 134 aa; highly similar to many e.g. RS11_BACSU 30S ribosomal protein S11 (131 aa), fasta scores; opt: 601 z-score: 1026.7 E(): 0, 69.8% identity in 129 aa overlap. Contains PS00054 Ribosomal protein S11 signature and Pfam match to entry PF00411 S11, Ribosomal protein S11, score 247.00, E-value 5.7e -76 2 1 15 12 [Reporter]SCO2544 (11B8)_PCR/823 [Gene]SCO2544/754 NC_003888 SCC77.11, possible IclR-family transcriptional regulator, len: 265 aa. Similar to many other IclR-family regulators including: Escherichia coli SW:ICLR_ECOLI (EMBL:M31761) acetate operon repressor IclR (274 aa), fasta scores opt: 368 z-score: 447.5 E(): 1.5e-17 33.1% identity in 263 aa overlap and Erwinia carotovora subsp. carotovora TR:AAD50647 (EMBL:AF135396) regulator of virulence KdgR (263 aa), fasta scores opt: 326 z-score: 397.5 E(): 9.4e-15 29.7% identity in 239 aa overlap. Contains a possible N-terminal helix-turn-helix motif, situated between residues 36..57 (+4.94 SD) and a Pfam match to entry PF01614 IclR. 2 2 9 19 [Reporter]SCO6052 (4F7)_PCR/2714 [Gene]SCO6052/2472 NC_003888 SC1B5.12c, putative membrane protein, len: 495 aa; similar to TR:Q9X9W6 (EMBL:AL096743) Streptomyces coelicolor hypothetical protein SCI7.24c, 490 aa; fasta scores: opt: 1059 Z-score: 1198.3 E(): 4.1e-59; 48.081% identity in 495 aa overlap. Contains possible hydrophobic membrane spanning region 2 1 15 11 [Reporter]SCO3940 (12N4)_PCR/822 [Gene]SCO3940/753 NC_003888 SCD78.07c, possible transmembrane protein, len: 301 aa; contains two possible membrane spanning regions 2 2 9 18 [Reporter]SCO5729 (5B7)_PCR/2713 [Gene]SCO5729/2471 NC_003888 SC3C3.15c, hypothetical protein, len: 745 aa; similar to TR:Q9AFG1 (EMBL:AF349618) Streptomyces ansochromogenes AG2 protein, 760 aa; fasta scores: pt: 640 Z-score: 664.3 E(): 2.3e-29; 29.462% identity in 706 aa overlap 2 1 15 10 [Reporter]SCO3437 (13J4)_PCR/821 [Gene]SCO3437/752 NC_003888 SCE36.04c, possible integral membrane protein, len: 246aa; contains possible membrane spanning hydrophobic regions. 2 2 9 17 [Reporter]SCO6668 (6N3)_PCR/2712 [Gene]SCO6668/2470 NC_003888 SC5A7.18c, probable two-component sensor, len: 405 aa; similar in C-terminus to many e.g. NARQ_ECOLI nitrate /nitrite sensor protein narQ (566 aa), fasta scores; opt: 230 z-score: 252.5 E(): 7.9e-07, 30.7% identity in 202 aa overlap. N-terminus contains six probable trans-membrane domains. Contains probable coiled-coil from 168 to 197 2 1 15 9 [Reporter]SCO3651 (14F4)_PCR/820 [Gene]SCO3651/751 NC_003888 SCH10.29c, hypothetical protein, len: 262 aa; unknown function, weakly similar to SW:YPHB_ECOLI (EMBL:AE000340), YphB, Escherichia coli hypothetical protein (290 aa), fasta scores; opt: 225 z-score: 270.0 E(): 1e-07, 26.4% identity in 239 aa overlap and SW:YIHR_ECOLI (EMBL:L19201), YihR, Escherichia coli hypothetical protein (308 aa) (26.3% identity in 270 aa overlap) 2 1 15 8 [Reporter]SCO0474 (15B4)_PCR/819 [Gene]SCO0474/750 NC_003888 SCF76.14c, possible lipoprotein, len: 415 aa. Contains an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. 3 4 20 19 [Reporter]SCO5590 (4C17)_PCR/7491 [Gene]SCO5590/6829 NC_003888 SC2E1.07, unknown, len: 198 aa 3 4 20 18 [Reporter]SCO7670 (5O13)_PCR/7490 [Gene]SCO7670/6828 NC_003888 SC4C2.05c, conserved hypothetical protein, len: 171 aa; similar to TR:O06539 (EMBL:Z95584) Mycobacterium tuberculosis hypothetical 19.0 kDa protein MTCI65.06c, 166 aa; fasta scores: opt: 697 z-score: 858.3 E(): 0; 62.4% identity in 165 aa overlap 3 4 20 17 [Reporter]SCO6740 (6K13)_PCR/7489 [Gene]SCO6740/6827 NC_003888 SC5F2A.23c, probable D-amino acid oxidase (secreted), len: 320 aa; similar to many both prokaryote and eukaryote egs. TR:O33145 (EMBL:AJ000521) putative D-amino acid oxidase from Mycobacterium tuberculosis (320 aa) fasta scores; opt: 805, z-score: 892.7, E(): 0, (43.4% identity in 316 aa overlap) and SW:OXDD_HUMAN D-aspartate oxidase from Homo sapiens (341 aa) fasta scores; opt: 548, z-score: 609.9, E(): 1.2e-26, (36.4% identity in 327 aa overlap). Contains Pfam match to entry PF01266 DAO, D-amino acid oxidase and Prosite match to PS00677 D-amino acid oxidases signature. Also contains probable N-terminal signal sequence. 3 4 20 16 [Reporter]SCO5642 (7G13)_PCR/7488 [Gene]SCO5642/6826 NC_003888 SC6A9.25, unknown, len: 254 aa; this ORF has atypically low GC bias in FramePlot 3 4 20 15 [Reporter]SCO5207 (8C13)_PCR/7487 [Gene]SCO5207/6825 NC_003888 SC7E4.04c, hypothetical protein, len: 145 aa; similar to TR:Q9YFH9 (EMBL:AP000058) Aeropyrum pernix hypothetical 15.7 kD protein APE0267, 143 aa; fasta scores: opt: 220 z-score: 282.8 E(): 2.8e-08; 34.1% identity in 126 aa overlap. Contains 2x Pfam match to entry PF00571 CBS, CBS domain 3 4 20 14 [Reporter]SCO6997 (9O9)_PCR/7486 [Gene]SCO6997/6824 NC_003888 SC8F11.23, unknown, len: 163 aa. The very N-terminus is Asp rich. 3 4 20 13 [Reporter]SCO2524 (10K9)_PCR/7485 [Gene]SCO2524/6823 NC_003888 SCC121.27c, unknown, len: 614 aa 3 4 20 12 [Reporter]SCO2560 (11G9)_PCR/7484 [Gene]SCO2560/6822 NC_003888 SCC77.27c, unknown, len: 792 aa. Weakly similar to Streptomyces coelicolor TR:O86807 (EMBL:AL031031) putative regulatory protein SC7C7.02C (916 aa), fasta scores opt: 447 z-score: 376.3 E(): 1.4e-13 34.1% identity in 954 aa overlap. The N-terminus is also similar Streptomyces coelicolor TR:Q53897(EMBL:X60316) OrfA within the AbaA pleiotropic regulatory locus for antibiotic production (192 aa), fasta scores opt: 248 z-score: 220.6 E(): 6.7e-05 37.1% identity in 178 aa overlap 3 3 3 14 [Reporter]SCO7591 (8K18)_PCR/5097 [Gene]SCO7591/4649 NC_003888 SC7H9.03c, probable regulatory protein, len: 320 aa; similar to SW:NAHR_PSEPU (EMBL:J04233) Pseudomonas putida transcriptional activator protein NahR, 300 aa; fasta scores: opt: 452 z-score: 510.2 E(): 7e-21; 32.4% identity in 296 aa overlap. Contains Pfam matches to entries PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and PF01046 NodD_C_term, NodD transcription activator carboxyl terminal region and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature 3 4 20 11 [Reporter]SCO4475 (12C9)_PCR/7483 [Gene]SCO4475/6821 NC_003888 SCD65.18, possible cytochrome biogenesis related protein, len: 380 aa; similar to SW:CCSA_GUITH (EMBL:X52159) Guillardia theta cytochrome biogenesis protein CcsA, 301 aa; fasta scores: opt: 455 z-score: 519.0 E(): 1.9e-21; 35.6% identity in 253 aa overlap. Contains Pfam match to entry PF01578 CytC_asm, Cytochrome C assembly protein and possible hydrophobic membrane spanning regions 3 3 3 13 [Reporter]SCO7147 (9G18)_PCR/5096 [Gene]SCO7147/4648 NC_003888 SC9A4.09, probable ketoreductase, len: 255 aa; similar to TR:Q9RN66 (EMBL:AF187532) Streptomyces nogalater C-7 ketoreductase, 281 aa; fasta scores: opt: 616 z-score: 709.0 E(): 5e-32; 40.3% identity in 243 aa overlap and to SW:FABG_THEMA (EMBL:AE001811) Thermotoga maritima 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) FabG, 246 aa; fasta scores: opt: 448 z-score: 519.1 E(): 1.9e-21; 34.1% identity in 246 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 3 4 20 10 [Reporter]SCO3267 (13O5)_PCR/7482 [Gene]SCO3267/6820 NC_003888 SCE39.17, unknown, len: 66aa; 3 3 3 12 [Reporter]SCO2456 (10C18)_PCR/5095 [Gene]SCO2456/4647 NC_003888 SCC24.27c, possible integral membrane protein, len: 146 aa; similar to SW:Y0B1_MYCTU (EMBL:Z77724) Mycobacterium tuberculosis hypothetical 13.7 kD protein CY227.01c, MTCY227.01c, 133 aa; fasta scores: opt: 241 z-score: 297.9 E(): 3.7e-09; 34.8% identity in 135 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 3 11 [Reporter]SCO4502 (11O14)_PCR/5094 [Gene]SCO4502/4646 NC_003888 SCD35.09, probable ketoacyl CoA thiolase, len: 428 aa; similar to TR:O86361 (EMBL:AL021929) Mycobacterium tuberculosis FadA2, 440 aa; fasta scores: opt: 1585 z-score: 1791.9 E(): 0; 63.0% identity in 446 aa overlap and to SW:THIK_ECOLI (EMBL:M87049) Escherichia coli 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (fatty oxidation complex beta subunit) (beta-ketothiolase) (acetyl-CoA acyltransferase) FadA, 387 aa; fasta scores: opt: 453 z-score: 515.8 E(): 2.7e-21; 30.9% identity in 430 aa overlap. Contains Pfam match to entry PF00108 thiolase, Thiolase 3 3 3 10 [Reporter]SCO3287 (12K14)_PCR/5093 [Gene]SCO3287/4645 NC_003888 SCE15.04, serine/arginine rich protein, len: 172aa; 3 3 3 9 [Reporter]SCO2869 (13G14)_PCR/5092 [Gene]SCO2869/4644 NC_003888 SCE6.06, possible regulatory protein, len: 281 aa. Similar to several proteins of undefined function e.g. Streptomyces albulus TR:BAA89264(EMBL: AB025200) plasmid pNO33 protein ORF312 (285 aa), fasta scores opt: 356 z-score: 441.0 E(): 3.9e-17 30.9% identity in 282 aa overlap. Also weakly similar to SCE6.02, also located on this cosmid, fasta scores opt: 269 z-score: 293.0 E(): 8.1e-11 27.5% identity in 265 aa overlap. Contains a putative helix-turn-helix motif situated between residues 27..48 (+4.07 SD). 3 3 3 8 [Reporter]SCO1657 (14C14)_PCR/5091 [Gene]SCO1657/4643 NC_003888 SCI46.02c, probable methionine synthase, len: 1170 aa; similar to SW:METH_ECOLI (EMBL:X16584) Escherichia coli 5-methyltetrahydrofolate-homocysteine methyltransferase (EC 2.1.1.13) (methionine synthase, vitamin-B12 dependent isozyme) MetH, 1226 aa; fasta scores: opt: 1593 z-score: 1749.1 E(): 0; 32.4% identity in 1215 aa overlap. Contains Pfam match to entry PF02310 B12-binding and match to Prosite entry PS00178 Aminoacyl-transfer RNA synthetases class-I signature 3 3 3 7 [Reporter]SCO0792 (15O10)_PCR/5090 [Gene]SCO0792/4642 NC_003888 SCF43.03, unknown, len: 343 aa. Almost identical to Streptomyces lividans TR:P72467(EMBL:Z70724) substrate hyphae associated protein, Orf4 (343 aa), fasta scores opt: 2274 z-score: 2584.7 E():0 99.1% identity in 343 aa overlap. Also weakly similar to Streptomyces coelicolor TR:Q9WX08(EMBL:AL079345) putative epimerase (353 aa), fasta scores opt: 275 z-score: 318.0 E(): 2.5e-10 25.9% identity in 340 aa overlap. 2 2 9 16 [Reporter]SCO3611 (7J3)_PCR/2711 [Gene]SCO3611/2469 NC_003888 SC66T3.22, putative membrane protein, len: 278 aa; unknown function, weakly similar to SW:Y01G_MYCTU (EMBL:Z70692) Mycobacterium tuberculosis hypothetical protein (271 aa), fasta scores; opt: 218 z-score: 233.1 E(): 1.2e-05, 26.8% identity in 254 aa overlap. Has a hydrophobic region at the N-terminus 3 3 3 6 [Reporter]SCO0483 (16K10)_PCR/5089 [Gene]SCO0483/4641 NC_003888 SCF34.02c, putative secreted protein, len: 133 aa; unknown function, possible CDS suggested by GC frameplot. Contains an Arg-rich region 2 2 9 15 [Reporter]SCO2161 (8F3)_PCR/2710 [Gene]SCO2161/2468 NC_003888 SC6G10.34c, conserved hypothetical protein, len: 118 aa; similar to many e.g. SW:YM04_MYCTU (EMBL:Z70283) protein RV2204c from Mycobacterium tuberculosis (118 aa) fasta scores; opt: 625, z-score: 766.6, E(): 0, (74.6% identity in 118 aa overlap). Contains PS01152 Hypothetical hesB/yadR/yfhF family signature 3 3 3 5 [Reporter]SCO7635 (17G10)_PCR/5088 [Gene]SCO7635/4640 NC_003888 SC10F4.08c, snp3, secreted extracellular small neutral protease, len: 182 aa; similar to SW:SNPA_STRLI (EMBL:M81703) Streptomyces lividans extracellular small neutral protease precursor (EC 3.4.24.-) SnpA, 227 aa; fasta scores: opt: 347 z-score: 398.6 E(): 1.1e-14; 40.7% identity in 140 aa overlap. Contains Pfam match to entry PF02031 Peptidase_M7, Streptomyces extracellular neutral proteinase (M7) family and match to Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Also contains possible N-terminal region signal peptide sequence 2 1 15 7 [Reporter]SCO3396 (15N24)_PCR/818 [Gene]SCO3396/749 NC_003888 SCE9.03c, possible membrane protein, len: 370 aa; unknown function, similar to SW:YLI2_CORGL (EMBL:X60312) Corynebacterium glutamicum hypothetical protein (426 aa), fasta scores; opt: 274 z-score: 307.1 E(): 8.8e-10, 23.9% identity in 351 aa overlap and TR:P94973 (EMBL:Z85982) Mycobacterium tuberculosis hypothetical protein (489 aa) (23.1% identity in 386 aa overlap). Contains hydrophobic regions at N-terminus, possibly a membrane anchor 2 2 9 13 [Reporter]SCO1510 (9N23)_PCR/2708 [Gene]SCO1510/2467 NC_003888 SC9C5.34, possible peptidyl-prolyl cis-trans isomerase, len: 277 aa; similar to SW:CYPB_MYCLE (EMBL:U00011) Mycobacterium leprae probable peptidyl-prolyl cis-trans isomerase PpiB or MLCB1259.10c, 295 aa; fasta scores: opt: 514 z-score: 573.6 E(): 1.8e-24; 40.0% identity in 295 aa overlap and to SW:CYPB_BACSU (EMBL:L09228) Bacillus subtilis peptidyl-prolyl cis-trans isomerase PpiB, 143 aa; fasta scores: opt: 240 z-score: 276.9 E(): 5.9e-08; 37.0% identity in 127 aa overlap. Contains Pfam match to entry PF00160 pro_isomerase, Cyclophilin type peptidyl-prolyl cis-trans isomerase. Contains also possible hydrophobic membrane spanning region 2 1 15 6 [Reporter]SCO1062 (16J24)_PCR/817 [Gene]SCO1062/748 NC_003888 SCG22.08c, possible hydrolase, len: 693 aa; similar to SW:BGLR_ECOLI (EMBL:M14641) Escherichia coli beta-glucuronidase (EC 3.2.1.31) GusA, 603 aa; fasta scores: opt: 386 z-score: 439.2 E(): 5.5e-17; 25.3% identity in 582 aa overlap and to TR:Q9RIU6 (EMBL: AL133278) Streptomyces coelicolor putative hydrolase SCM11.15c, 872 aa; fasta scores: opt: 977 z-score: 1111.4 E(): 0; 37.1% identity in 596 aa overlap 2 2 9 12 [Reporter]SCO2769 (10J23)_PCR/2707 [Gene]SCO2769/2466 NC_003888 SCC57A.40c, possible acetolactate synthase, len: 564 aa. Similar to many e.g. Escherichia coli SW:ILVB_ECOLI (EMBL:J01633) acetolactate synthase isozyme I large subunit (EC 4.1.3.18) (562 aa), fasta scores opt: 732 z-score: 776.9 E(): 0 29.6% identity in 538 aa overlap. Contains a Prosite hit to PS00187 Thiamine pyrophosphate enzymes signature and a Pfam match to entry PF00205 TPP_enzymes, Thiamine pyrophosphate enzymes 2 1 15 5 [Reporter]SCO0840 (17F24)_PCR/816 [Gene]SCO0840/747 NC_003888 SCF43A.30c, possible marR-family transcriptional regulator, len: 168 aa; weakly similar to several transcriptional regulators e.g. SW:PECS_ERWCH (EMBL:X74409), PecS, Erwinia chrysanthemi regulator of pectinase, cellulase, and blue pigment production (166 aa), fasta scores; opt: 125 z-score: 166.0 E(): 0.064, 29.2% identity in 89 aa overlap. Also similar to hypothetical proteins e.g. TR:O31472 (EMBL:Z99105) Bacillus subtilis hypothetical protein (155 aa) (35.4% identity in 144 aa overlap). Contains Pfam match to PF01047 MarR, MarR family Score 30.03 2 2 9 11 [Reporter]SCO4677 (11F23)_PCR/2706 [Gene]SCO4677/2465 NC_003888 SCD31.02c, possible regulatory protein, len: 144 aa; similar to TR:CAB60156 (EMBL:AL132824) Streptomyces coelicolor putative regulatory protein SCAH10.02, 160 aa; fasta scores: opt: 396 z-score: 500.7 E(): 1.9e-20; 51.7% identity in 143 aa overlap 2 1 15 4 [Reporter]SCO0141 (18B24)_PCR/815 [Gene]SCO0141/746 NC_003888 SCJ33.05c, possible calcium-binding protein, len: 183 aa. Weakly similar to many Eukaryotic calcium-binding proteins e.g. Drosophila melanogaster (Fruit fly) TR:O16158 (EMBL:AF014952) calcium-binding protein (184 aa), fasta scores opt: 265 z-score: 323.1 E(): 1.2e-10 28.1% identity in 178 aa overlap. Contains a 4xPfam match to entry PF00036 efhand, EF hand. 2 2 9 10 [Reporter]SCO3391 (12B23)_PCR/2705 [Gene]SCO3391/2464 NC_003888 SCE126.09, unknown,len: 362aa; similar to TR:O25492 (EMBL:AE000593) hypothetical protein from Helicobacter pylori (336 aa) fasta scores; opt: 143, z-score: 161.4, E(): 0.11, (31.5% identity in 111 aa overlap). 2 1 15 3 [Reporter]SCO5357 (19N20)_PCR/814 [Gene]SCO5357/745 NC_003888 SCBAC5H2.26, rho, transcription terminator factor (fragment), len: >344 aa; highly similar to N-terminal region of SW:RHO_STRLI (EMBL:X95444) Streptomyces lividans transcription terminator factor Rho, 707 aa; fasta scores: opt: 1705 z-score: 1432.0 E(): 0; 95.8% identity in 357 aa overlap,2SC6G5.01, rho, transcription termination factor, partial CDS, len: >383aa; strongly similar to many eg. SW:P03002 (RHO_ECOLI) transcription termination factor Rho from Escherichia coli (419 aa) fasta scores; opt: 1432, z-score: 1584.9, E(): 0, 57.3% identity in 370 aa overlap. Almost identical to C-terminal portion of SW:P52157 (RHO_STRLI) transcription termination factor Rho from Streptomyces lividans (707 aa) fasta scores; opt: 2468, z-score: 2723.8, E(): 0, 99.7% identity in 383 aa overlap. Contains Pfam match to entry PF00006 ATP-synt_ab, ATP synthase alpha/beta family and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 2 2 9 9 [Reporter]SCO0841 (13N19)_PCR/2704 [Gene]SCO0841/2463 NC_003888 SCF43A.31, possible oxidoreductase, len: 520 aa; weakly similar to some oxidoreductases e.g. SW:NUEM_BOVIN (EMBL:X59418) Bos taurus NADH-ubiquinone oxidoreductase 39 kD subunit precursor (380 aa), fasta scores; opt: 288 z-score: 319.2 E(): 1.9e-10, 23.8% identity in 286 aa overlap. Also similar to hypothetical proteins e.g. SW:YBJT_ECOLI (EMBL:AE000188) Escherichia coli hypothetical protein (476 aa) (32.2% identity in 490 aa overlap). The N-terminal half is similar to TR:O54156 (EMBL:AL021409) S.coelicolor probable oxidoreductase (347 aa) (31.7% identity in 287 aa overlap) 2 1 15 2 [Reporter]SCO5989 (20F20)_PCR/813 [Gene]SCO5989/744 NC_003888 SCBAC16H6.24c, possible integral membrane protein, len: 294aa: similar to TR:Q9K400 (EMBL:AL359949) putative integral membrane protein 2SCG61.15 from Streptomyces coelicolor (322 aa) fasta scores; opt: 900, Z-score: 931.3, 52.597% identity (59.124% ungapped) in 308 aa overlap. Contains possible membrane-spanning hydrophobic regions 2 2 9 8 [Reporter]SCO3381 (14J19)_PCR/2703 [Gene]SCO3381/2462 NC_003888 SCE94.32c, nadC, nicotinate-nucleotide pyrophophorylase, len: 329 aa; similar to many e.g. SW:NADC_MYCTU NadC, nicotinate-nucleotide pyrophophorylase from Mycobacterium tuberculosis (285 aa) fasta scores; opt: 909, z-score: 1017.8, E(): 0, (57.3% identity in 267 aa overlap). 2 1 14 22 [Reporter]SCO1013 (1F16)_PCR/811 [Gene]SCO1013/743 NC_003888 2SCG2.26c, possible mut-like protein, len: 159 aa; similar to TR:Q9K3X1 (EMBL:AL359988) Streptomyces coelicolor putative mut-like protein SCD10.05c, 184 aa; fasta scores: opt: 203 z-score: 264.6 E(): 3.2e-07; 35.7% identity in 126 aa overlap. Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein and match to Prosite entry PS00893 mutT domain signature 2 2 9 7 [Reporter]SCO0154 (15F19)_PCR/2702 [Gene]SCO0154/2461 NC_003888 SCJ1.03c, unknown,len:220 aa, similarity to Synechocystis sp. (strain PCC 6803) hypothetical 24.8 KD protein TR:P73776 (EMBL: D90909)(218 aa), fasta scores opt: 291 z-score: 344.1 E(): 8.3e-12 31.2% identity in 170 aa overlap and a weakly similar to Pseudomonas fluorescens SW:TIPB_PSEFL (EMBL; U44827) thiol:disulfide interchange protein TipB precursor (cytochrome C biogenesis protein TipB) (178 aa), fasta scores opt: 148 z-score: 181.8 E(): 0.0091 28.7% identity in 122 aa overlap. 2 1 14 21 [Reporter]SCO0961 (2B16)_PCR/810 [Gene]SCO0961/742 NC_003888 SCM11.16c, glgC, glucose-1-phosphate adenylyltransferase (EC 2.7.7.27), len: 397 aa; identiocal to previously sequenced SW:GLGC_STRCO (EMBL:X89733) Streptomyces coelicolor glucose-1-phosphate adenylyltransferase (EC 2.7.7.27) (ADP-glucose synthase) (ADP-glucose pyrophosphorylase) GlgC, 397 aa and similar to SW:GLGC_ECOLI (EMBL:V00281) Escherichia coli glucose-1-phosphate adenylyltransferase (EC 2.7.7.27) (ADP-glucose synthase) (ADP-glucose pyrophosphorylase) GlgC, 430 aa; fasta scores: opt: 1017 z-score: 1092.0 E(): 0; 39.3% identity in 397 aa overlap. Contains Pfam match to entry PF00483 NTP_transferase, Nucleotidyl transferase and four matchs to Prosite entries PS00808 ADP-glucose pyrophosphorylase signature 1, PS00809 ADP-glucose pyrophosphorylase signature 2, PS00810 ADP-glucose pyrophosphorylase signature 3 and PS00061 Short-chain dehydrogenases/reductases family signature 2 2 9 6 [Reporter]SCO0348 (16B19)_PCR/2701 [Gene]SCO0348/2460 NC_003888 SCF41.07, possible 2-hydroxyacid-family dehydrogenase, len: 339 aa. Similar to Mycobacterium leprae SW:SERA_MYCLE (EMBL; Z99263) D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) (PGDH) MLCB637.25 (528 aa), fasta scores opt: 452 z-score: 525.4 E(): 6.5e-22 36.7% identity in 286 aa overlap and Pyrococcus abyssi TR:CAB49675 (EMBL; AJ248285) SERA-like protein (307 aa), fasta scores opt: 437 z-score: 511.6 E(): 3.8e-21 32.0% identity in 309 aa overlap. Contains a Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases 2 1 14 20 [Reporter]SCO5928 (3N12)_PCR/809 [Gene]SCO5928/741 NC_003888 SC10A5.33c, unknown, len: 234 aa 2 1 14 19 [Reporter]SCO7028 (4J12)_PCR/808 [Gene]SCO7028/740 NC_003888 SC1H10.17, possible sugar-binding lipoprotein, len: 434 aa. Identical to Streptomyces lividans TR:Q9Z492 (EMBL:AF043654) putative sugar-binding protein, BxlE (434 aa), fasta scores opt: 2892 z-score: 3295.8 E():0 99.5% identity in 434 aa overlap. Also similar to Streptococcus mutans SW:MSME_STRMU(EMBL:M77351) multiple sugar-binding protein precursor, MsmE (420 aa), fasta scores opt: 308 z-score: 353.5 E(): 2.9e-12 24.0% identity in 383 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains a Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein. 3 4 20 9 [Reporter]SCO5891 (14K5)_PCR/7481 [Gene]SCO5891/6819 NC_003888 St3F7.11, redM, probable peptide synthase, len: 532 aa; similar to part of many peptide synthases eg. Myxococcus xanthus TR:Q50858 (EMBL:U24657) saframycin MX1 synthetase A (2605 aa), fasta scores; opt: 572 z-score: 782.6 E(): 0, 36.1% identity in 548 aa overlap. Contains PS00455 Putative AMP-binding domain signature and Pfam match to entry AMP-binding PF00501, AMP-binding enzymes, score 392.99. Also similar to part of SC3F7.12 aa 60 to 590 (E(): 2.8e-05, 25.3% identity in 561 aa overlap) 3 4 20 8 [Reporter]SCO3410 (15G5)_PCR/7480 [Gene]SCO3410/6818 NC_003888 SCE9.17, possible membrane protein, len: 578 aa; similar to TR:O69704 (EMBL:AL022121) Mycobacterium tuberculosis putative membrane protein (529 aa), fasta scores; opt: 642 z-score: 677.3 E(): 2.1e-30, 31.6% identity in 481 aa overlap. The start codon is uncertain as there are 2 potential. The general position of the CDS start was estimated by GC frame plot. Contains hydrophobic, possible membrane-spanning regions in the C-terminal half, while the N-terminal half is more hydrophilic 3 4 20 7 [Reporter]SCO3256 (16C5)_PCR/7479 [Gene]SCO3256/6817 NC_003888 SCE39.06c, SpdA protein, len: 217 aa; highly similar to TR:Q07193 (EMBL:AJ005260) SpdA protein form the integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens (224 aa) fasta scores; opt: 1071, z-score: 973.6, E(): 0, (75.6% identity in 225 aa overlap). Contains possible membrane spanning regions and a possible N-terminal region signal sequence peptide 3 4 20 6 [Reporter]SCO0540 (17O1)_PCR/7478 [Gene]SCO0540/6816 NC_003888 SCF11.20, probable sugar transport membrane protein, len: 299 aa; similar to TR:Q06111 (EMBL:X66092) Clostridium perfringens putative transport system permease protein, 275 aa; fasta scores: opt: 704 z-score: 838.6 E(): 0; 38.4% identity in 255 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, binding-protein-dependent transport systems inner membrane component and a prosite match to entry PS00402 binding-protein-dependent transport systems inner membrane component signature. Contains porbable N-terminal signal sequence 3 4 20 5 [Reporter]SCO3630 (18K1)_PCR/7477 [Gene]SCO3630/6815 NC_003888 SCH10.08, hypothetical protein, len: 301 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains a hydrophobic, possible membrane-spanning region 3 4 20 4 [Reporter]SCP1.318 (19G1)_PCR/7476 [Gene]SCP1.318/6814 NC_003888 SCP1.318, possible AraC-family transcriptional regulator, len: 304aa; previously sequenced as TR:Q9L8G9 (EMBL:AF204735). Similar to many eg. from streptomycetes eg. SW:Q03320 (ARAL_STRAT) putative AraC-like transcription regulator from Tn4811-homologous locus in Streptomyces antibioticus (303 aa) fasta scores; opt: 1769, z-score: 2065.7, E(): 0, 88.5% identity in 304 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family, Prosite match to PS00041 Bacterial regulatory proteins, araC family signature and helix-turn-helix motif (Score 1345 (+3.77 SD)) at residue 216-237. 3 4 20 3 [Reporter]SCO4020 (20C1)_PCR/7475 [Gene]SCO4020/6813 NC_003888 2SC10A7.24, probable two component system response regulator, len: 271 aa; similar to TR:O69730 (EMBL:AL022121) Mycobacterium tuberculosis putative two-component system regulator MTV025.113c, 234 aa; fasta scores: opt: 1111 z-score: 1309.2 E(): 0; 72.3% identity in 231 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 3 4 20 2 [Reporter]SCO7475 (20K21)_PCR/7474 [Gene]SCO7475/6812 NC_003888 SCBAC17A6.08, possible phenylacetic acid degradation NADH oxidoreductase PaaE, len: 368 aa; similar to many e.g. SW:P76081 (PAAE_ECOLI) probable phenylacetic acid degradation NADH oxidoreductase PaaE from Escherichia coli (356 aa) fasta scores; opt: 871, Z-score: 943.6, 39.943% identity (40.870% ungapped) in 353 aa overlap. Contains Pfam match to entry PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains; Pfam match to entry PF00175 NAD_binding, Oxidoreductase FAD/NAD-binding domain; Pfam match to entry PF00970 FAD_binding_6, Oxidoreductase FAD-binding domain and Prosite match to PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. 3 3 3 4 [Reporter]SCO2956 (18C10)_PCR/5087 [Gene]SCO2956/4639 NC_003888 SCE59.15, possible integral-membrane protein, len: 187 aa; similar to TR:CAB66271 (EMBL:AL136519) Streptomyces coelicolor putative integral-membrane protein SCC57A.03c, 199 aa; fasta scores: opt: 333 z-score: 376.0 E(): 1.6e-13; 47.3% identity in 182 aa overlap. Contains possible hydrohpobic membrane spanning regions 3 4 19 22 [Reporter]SCO1330 (1K17)_PCR/7472 [Gene]SCO1330/6811 NC_003888 2SCG61.12c, possible fatty acid CoA ligase, len: 360aa; region similar to many possible fatty acid coA ligases eg. TR:Q9ZIP5 (EMBL:AF117694) malonyl CoA synthetase from Rhizobium trifolii (504 aa) fasta scores; opt: 198, z-score: 230.9, E(): 2.2e-05, 28.6% identity in 203 aa overlap. 3 3 3 3 [Reporter]SCO4060 (19O6)_PCR/5086 [Gene]SCO4060/4638 NC_003888 2SCD60.26, unknown, len: 136 aa. Contains TTA leucine codon (136), possible target for bldA regulation 3 4 19 21 [Reporter]SCO5777 (2G17)_PCR/7471 [Gene]SCO5777/6810 NC_003888 SC4H8.16c, gluA, probable glutamate uptake system ATP-binding protein, len: 258 aa; highly similar to many amino-acid uptake ATP-binding proteins eg. GLUA_CORGL P48243 glutamate transport atp-binding protein from Corynebacterium gluamicum (242 aa), fasta scores; opt: 1152 z-score: 1564.5 E(): 0, 71.5% identity in 242 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 3 3 2 [Reporter]SCO4018 (20G6)_PCR/5085 [Gene]SCO4018/4637 NC_003888 2SC10A7.22c, conserved hypothetical protein, len: 177 aa; highly similar to SW:YL40_MYCTU (EMBL:Z95388) Mycobacterium tuberculosis hypothetical 18.6 kDa protein MTCY270.28, 176 aa; fasta scores: opt: 672 z-score: 724.7 E(): 7.4e-33; 57.7% identity in 175 aa overlap. Contains Pfam match to entry PF01161 PBP, Phosphatidylethanolamine-binding protein 3 3 2 22 [Reporter]SCO5191 (1G2)_PCR/5083 [Gene]SCO5191/4636 NC_003888 2SC3B6.15, unknown, len: 107 aa 3 3 2 21 [Reporter]SCO0730 (2C2)_PCR/5082 [Gene]SCO0730/4635 NC_003888 3SC5B7.08c, conserved hypothetical protein, len: 153 aa; similar to TR:O86340 (EMBL:Z97984) Mycobacterium tuberculosis hypothetical 14.8 kDa protein MTV019.02c, 134 aa; fasta scores: opt: 309 z-score: 388.9 E(): 3.9e-14; 39.0% identity in 123 aa overlap 3 3 2 20 [Reporter]SCO5805 (2O22)_PCR/5081 [Gene]SCO5805/4634 NC_003888 SC4H2.26, nrdJ, ribonucleotide reductase, len: 967 aa; highly similar to Streptomyces clavuligerus TR:E1258368(EMBL:AJ224870) vitamin B12-dependent ribonucleotide reductase (951 aa), fasta scores; opt: 5800 z-score: 5797.4 E():0, 91.3% identity in 958 aa overlap. Contains 2x match to Pfam match to entry ribonucleo_red PF00317, Ribonucleotide reductase, scores 75.54 and 86.71 3 3 2 19 [Reporter]SCO1402 (3K22)_PCR/5080 [Gene]SCO1402/4633 NC_003888 SC1A8A.22c, cvnA4, possible large secreted protein, len: 943aa; similar to others from Streptomyces coelicolor and Mycobacterium tuberculosis eg. TR:Q9X836 (EMBL:AL049727) putative large secreted protein from Streptomyces coelicolor (877 aa) fasta scores; opt: 1356, z-score: 1302.5, E(): 0, 34.5% identity in 887 aa overlap. Local genetic arrangement also seems to be conserved for the Streptomyces coelicolor paralogues. Contains a possible non-cleavable N-terminal signal sequence. 3 3 2 18 [Reporter]SCO6189 (4G22)_PCR/5079 [Gene]SCO6189/4632 NC_003888 SC2G5.10, possible transferase, len: 317aa; similar to many eg. SW:EXOU_RHIME succinoglycan biosynthesis protein, ExoU from Rhizobium meliloti (342 aa) fasta scores; opt:203, z-score: 234.7, E(): 8.7e-06, (30.3% identity in 274aa overlap). Pfam match to entry PF00535 Glycos_transf_2,Glycosyl transferases, score 37.70, E-value 2.6e-07. NB this CDS is an imperfect duplicate of the neighbouring downstream CDS SC2G5.11 fasta scores; opt: 772, z-score: 700.7, E():3.3e-34, (47.5% identity in 280 aa overlap). 2 2 9 5 [Reporter]SCO3740 (17N15)_PCR/2700 [Gene]SCO3740/2459 NC_003888 SCH22A.18c, probable histidine kinase, len: 370 aa; similar to TR:CAB54569 (EMBL:AJ006392) Streptococcus pneumoniae histidine kinase HK02, 449 aa; fasta scores: opt: 425 z-score: 452.2 E(): 9.2e-18; 33.7% identity in 285 aa overlap and to SW:RESE_BACSU (EMBL:L09228) Bacillus subtilis sensor protein ResE (EC 2.7.3.-), 589 aa; fasta scores: opt: 434 z-score: 460.1 E(): 3.4e-18; 35.8% identity in 229 aa overlap. Contains Pfam match to entry PF00512 signal, Histidine kinase. Contains also possible coiled-coil region at residues 83..95. Contains possible hydrophobic membrane spanning regions 3 3 2 17 [Reporter]SCO7126 (5C22)_PCR/5078 [Gene]SCO7126/4631 NC_003888 SC4B10.27, unknown, len: 150 aa 2 2 9 4 [Reporter]SCO1366 (18J15)_PCR/2699 [Gene]SCO1366/2458 NC_003888 SC10A9.08c, hypothetical protein, len: 219 aa; similar to TR:CAC05955 (EMBL:AL391763) Streptomyces coelicolor hypothetical 40.1 kDa protein 2SC7G11.14c, 360 aa; fasta scores: opt: 237 z-score: 225.1 E(): 4.3e-05; 34.5% identity in 145 aa overlap and to TR:Q9CB82 (EMBL:AL583925) Mycobacterium leprae hypothetical protein ML2313, 200 aa; fasta scores: opt: 526 Z-score: 466.8 E(): 2.3e-18; 49.171% identity in 181 aa overlap. Contains C-terminal region rich in glycine, arginine and proline amino acid residues 3 3 2 16 [Reporter]SCO7293 (6O18)_PCR/5077 [Gene]SCO7293/4630 NC_003888 SC5F8.03c, hypothetical protein, len: 225 aa. Similar to several proteins of undefined function including: Deinococcus radiodurans TR:Q9RWP1 (EMBL:AE001920) conserved hypothetical protein (254 aa), fasta scores opt: 467 z-score: 555.6 E(): 1.8e-23 36.6% identity in 205 aa overlap and Streptomyces coelicolor TR:CAB70638 (EMBL:AL137242) SC8F4.12C (239 aa), fasta scores opt: 397 z-score: 474.2 E(): 6.1e-19 36.1% identity in 183 aa overlap 2 1 14 18 [Reporter]SCO5463 (5F12)_PCR/807 [Gene]SCO5463/739 NC_003888 SC3D11.20, possible MerR-family transcriptional regulator, len: 304 aa; similar to many e.g. TRNEW:CAB56383 (EMBL:AL118515) putative MerR-family transcriptional regulator from Streptomyces coelicolor (319 aa) fasta scores; opt: 622, z-score: 719.6, E(): 1.2e-32, 41.3% identity in 310 aa overlap. Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family 2 2 9 3 [Reporter]SCO1373 (19F15)_PCR/2698 [Gene]SCO1373/2457 NC_003888 SC10A9.15c, conserved hypothetical protein, len: 505 aa; similar to TR:O69666 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical 56.9 kDa protein MTV025.046, 509 aa; fasta scores: opt: 1720 z-score: 2047.1 E(): 0; 53.6% identity in 494 aa overlap 2 1 14 17 [Reporter]SCO5806 (6B12)_PCR/806 [Gene]SCO5806/738 NC_003888 SC4H2.27c, hypotehtical protein, len: 202 aa; contains PS00017 ATP/GTP-binding site motif A (P-loop). Start site uncertain. Similar to TR:CAC37539 (EMBL:AL590982) Streptomyces coelicolor conserved hypothetical protein SCIF3.20c, 190 aa; fasta scores: opt: 685 Z-score: 693.9 E(): 5.1e-31; 56.111% identityin 180 aa overlap 2 2 9 2 [Reporter]SCO7497 (20N11)_PCR/2697 [Gene]SCO7497/2456 NC_003888 SCBAC17A6.30, possible transcriptional regulator, len: 259 aa; similar to many e.g. TR:Q9I5E7 (EMBL:AE004514) probable transcriptional regulator from Pseudomonas aeruginosa (262 aa) fasta scores: opt: 796, Z-score: 924.9, 48.077% identity in 260 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family and Prosite match to PS00041 Bacterial regulatory proteins, araC family signature. 2 1 14 16 [Reporter]SCO6771 (7N8)_PCR/805 [Gene]SCO6771/737 NC_003888 SC6A5.20, possible small hydrophobic secreted protein, len: 39 aa; contains possible N-terminal signal sequence. 2 2 8 22 [Reporter]SCO4226 (1N7)_PCR/2695 [Gene]SCO4226/2455 NC_003888 2SCD46.40c, unknown, len: 82aa; 2 1 14 15 [Reporter]SCO2481 (8J8)_PCR/804 [Gene]SCO2481/736 NC_003888 SC7A8.20c, hypothetical protein, len: 129 aa; similar to various hypothetical proteins, e.g. SW:YYBR_BACSU (EMBL:D26185) Bacillus subtilis hypothetical 14.7 kD protein in CotF-TetB intergenic region YybR, 125 aa; fasta scores: opt: 333 z-score: 424.6 E(): 2.9e-16; 45.5% identity in 112 aa overlap and to Streptomyces coelicolor SC7A1.18c, 141 aa; fasta scores: opt: 243 z-score: 270.7 E(): 1.1e-09; 49.4% identity in 77 aa overlap. Contains Pfam match to entry PF01638 DUF24, Protein of unknown function 2 2 8 21 [Reporter]SCO1851 (2J7)_PCR/2694 [Gene]SCO1851/2454 NC_003888 SCI8.36, cobO, cob(I)alamin adenosyltransferase (EC 2.5.1.17), len: 199 aa. Highly similar to many including: Pseudomonas denitrificans SW:COBO_PSEDE (EMBL: M62866) cob(I)alamin adenosyltransferase (EC 2.5.1.17) (corrinoid adenosyltransferase) (213 aa), fasta scores opt: 418 z-score: 517.2 E(): 1.9e-21 37.0% identity in 200 aa overlap and Mycobacterium tuberculosis TR:O05810 (EMBL: Z95207) cob(I)alamin adenosyltransferase CobA (207 aa), fasta scores opt: 918 z-score: 1124.4 E(): 0 64.7% identity in 207 aa overlap. 2 1 14 14 [Reporter]SCO5490 (9F8)_PCR/803 [Gene]SCO5490/735 NC_003888 SC8D9.02c, small conserved hypothetical protein, len: 123aa; similar to several eg. TR:P96817 (EMBL:Z92770) hypothetical protein from Mycobacterium tuberculosis (126 aa) fasta scores; opt: 279, z-score: 348.2, E(): 4.4e-12, (42.7% identity in 110 aa overlap). 2 2 8 20 [Reporter]SCO1644 (3F7)_PCR/2693 [Gene]SCO1644/2453 NC_003888 SCI41.27, pcrB 20S proteasome beta-subunit precursor, len: 281 aa. Identical to TR:O87598 (EMBL; AF086832) Streptomyces coelicolor 20S proteasome beta-subunit precursor 281 aa and similar to TR:Q53083 (EMBL; U26422) Rhodococcus sp. proteasome beta-type subunit 2, 292 aa; fasta socres: opt: 952 z-score: 1081.0 E():0; 56.7% identity in 277 aa overlap. 2 1 14 13 [Reporter]SCO7352 (10B8)_PCR/802 [Gene]SCO7352/734 NC_003888 SC9H11.06, possible membrane protein, len: 126 aa. Similar to several proteins of undefined function from Deinococcus radiodurans e.g. TR:AAF11579(EMBL:AE002039) hypothetical 13.2 kd protein (120 aa), fasta scores opt: 304 z-score: 370.4 E(): 3.6e-13 48.2% identity in 112 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 2 2 8 19 [Reporter]SCO6048 (4B7)_PCR/2692 [Gene]SCO6048/2452 NC_003888 SC1B5.08, probable integral membrane protein, len: 224 aa; contains six possible transmembrane domains. 2 1 14 12 [Reporter]SCO5281 (11N4)_PCR/801 [Gene]SCO5281/733 NC_003888 SCCB12.05c, probable 2-oxoglutarate dehydrogenase, len: 1272 aa; similar to TR:P96746 (EMBL:D84102) Corynebacterium glutamicum 2-oxoglutarate dehydrogenase (EC 1.2.4.2) OdhA, 1257 aa; fasta scores: opt: 4321 z-score: 3452.7 E(): 0; 54.8% identity in 1262 aa overlap and to SW:ODO1_ECOLI (EMBL:J01619) Escherichia coli 2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2) SucA, 933 aa; fasta scores: opt: 2568 z-score: 2056.0 E(): 0; 46.5% identity in 881 aa overlap. Contains Pfam matches to entries PF00198 2-oxoacid_dh, 2-oxo acid dehydrogenases acyltransferase (catalytic domain) and PF00676 E1_dehydrog, Dehydrogenase E1 component. Possible alternative start codon at codon 156 2 2 8 18 [Reporter]SCO5890 (5N3)_PCR/2691 [Gene]SCO5890/2451 NC_003888 SC3F7.10, redN, putative 8-amino-7-oxononanoate synthase, len: 640 aa; similar to many diverse transferases eg. BIOF_BACSH P22806 8-amino-7-oxononanoate synthase (389 aa), fasta scores; opt: 926 z-score: 888.4 E(): 0, 42.1% identity in 361 aa overlap. Also simlar to SC3F7.12 from aa 1700 to end (E(): 3.6e-15, 31.9% identity in 630 aa overlap). Contains Pfam match to entry aminotran_2 PF00222, Aminotransferases class-II, score 292.92 in C-terminus 2 1 14 11 [Reporter]SCO4133 (12J4)_PCR/800 [Gene]SCO4133/732 NC_003888 SCD72A.19, possible integral membrane protein, len: 238aa; N-terminal region similar to others eg. TR:O86625 (EMBL:AL031155) putative integral membrane protein from Streptomyces coelicolor (201 aa) fasta scores; opt: 321, z-score: 379.3, E(): 1.1e-13, 35.8% identity in 179 aa overlap. Contains possible membrane-spanning hydrophobic regions and Pfam match to entry PF01569 PAP2, PAP2 superfamily 2 2 8 17 [Reporter]SCO6664 (6J3)_PCR/2690 [Gene]SCO6664/2450 NC_003888 SC5A7.14, probable transcriptional regulator, len: 301 aa; some similarity to many members of the araC family, e.g. MMSR_PSEAE MmsAB operon regulatory protein (307 aa), fasta scores; opt: 113 z-score: 251.4 E(): 9.2e-07, 25.4% identity in 303 aa overlap. Contains probable helix-turn-helix motif from aa 216-237 (Score 1252, +3.45 SD) and Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family, score 63.30, E-value 5.3e-15 2 1 14 10 [Reporter]SCO3201 (13F4)_PCR/799 [Gene]SCO3201/731 NC_003888 SCE22.18c, probable tetR-family transcriptional regulator, len: 236 aa; similar to TR:CAB62751 (EMBL:AL133424) Streptomyces coelicolor putative tetR-family transcriptional regulator SCF56.06, 213 aa; fasta scores: opt: 311 z-score: 346.2 E(): 8e-12; 38.1% identity in 194 aa overlap and to SW:TCMR_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C transcriptional repressor TcmR, 226 aa; fasta scores: opt: 254 z-score: 284.8 E(): 2.1e-08; 32.2% identity in 214 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 53..74 (+5.22 SD) 2 1 14 9 [Reporter]SCO2983 (14B4)_PCR/798 [Gene]SCO2983/730 NC_003888 SCE50.11, possible glycosyl transferase, len: 745 aa; similar to TR:CAB66267 (EMBL:AL136518) Streptomyces coelicolor putative glycosyl transferase (fragment) SCC123.27, 743 aa; fasta scores: opt: 2355 z-score: 2721.3 E(): 0; 51.2% identity in 732 aa overla and to SW:TAGF_BACSU (EMBL:X15200) Bacillus subtilis teichoic acid biosynthesis protein F, TagF, 746 aa; fasta scores: opt: 553 z-score: 636.4 E(): 5.3e-28; 28.2% identity in 432 aa overlap. Also similar to Streptomyces coelicolor SCE50.09, 765 aa; fasta scores: opt: 2095 z-score: 1969.9 E(): 0; 48.7% identity in 764 aa overlap and SCE50.10, 736 aa; fasta scores: opt: 1407 z-score: 1323.0 E(): 0; 43.7% identity in 746 aa overlap. Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases 3 4 19 20 [Reporter]SCO7639 (3C17)_PCR/7470 [Gene]SCO7639/6809 NC_003888 SC10F4.12, probable marR-family regulatory protein, len: 140 aa; similar to TR:AAG06455 (EMBL:AE004731) Pseudomonas aeruginosa probable transcriptional regulator PA3067, 147 aa; fasta scores: opt: 336 z-score: 402.2 E(): 7.3e-15; 43.0% identity in 142 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 3 4 19 19 [Reporter]SCO7204 (4O13)_PCR/7469 [Gene]SCO7204/6808 NC_003888 SC2H12.03c, unknown, len: 64 aa. 3 4 19 18 [Reporter]SCO6566 (5K13)_PCR/7468 [Gene]SCO6566/6807 NC_003888 SC3F9.01, unknown, partial CDS, len: >403 aa; similar to many members of the ROK (nagC/xylR) family e.g. NAGC_ECOLI N-acetylglucosamine repressor (406 aa), fasta scores; opt: 513 z-score: 578.8 E(): 5e-25, 28.6% identity in 388 aa overlap and GLK_STRCO glucokinase (EC 2.7.1.2) (glucose kinase) (317 aa), fasta scores; opt: 544 z-score: 570.9 E(): 1.4e-24, 34.4% identity in 317 aa overlap. Contains PS01125 ROK family signature and Pfam match to entry ROK PF00480, ROK family, score 164.11. Also similar to S. coelicolor SC7B7.05, probable transcriptional repressor (E(): 9.6e-25, 34.3% identity in 385 aa overlap),SC4B5.16, possible ROK family protein, partial CDS, len: 92aa; forms the C-terminal region of TR:O69941 (EMBL:AL023862) partial CDS, hypothetical ROK family protein on adjacent cosmid 3F9 from Streptomyces coelicolor (403 aa). 3 4 19 17 [Reporter]SCO7754 (6G13)_PCR/7467 [Gene]SCO7754/6806 NC_003888 SC5E9.02, possible anti-sigma factor antagonist, len: 127aa; weakly similar to many eg. SW:Q9WVX8 (RSBV_STRCO) anti-sigma B factor antagonist from Streptomyces coelicolor (113 aa) fasta scores; opt: 118, z-score: 164.6, E(): 0.11, 27.7% identity in 94 aa overlap. Contains Pfam match to entry PF01740 STAS, STAS domain. 3 4 19 16 [Reporter]SCO5646 (7C13)_PCR/7466 [Gene]SCO5646/6805 NC_003888 SC6A9.21c, probable solute binding lipoprotein, len: 373 aa; similar to e.g. TBPA_ECOLI thiamin-binding periplasmic protein (327 aa), fasta scores; opt: 220 z-score: 423.3 E(): 2.4e-16, 28.4% identity in 327 aa overlap. Contains N-terminla signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 3 4 19 15 [Reporter]SCO2494 (8O9)_PCR/7465 [Gene]SCO2494/6804 NC_003888 SC7A8.33c, probable pyruvate phosphate dikinase, len: 909 aa; similar to TR:CAB53432 (EMBL:AL109989) Streptomyces coelicolor pyruvate phosphate dikinase SCJ12.20, 898 aa; fasta scores: opt: 3956 z-score: 4354.7 E(): 0; 66.7% identity in 889 aa overlap and to SW:PODK_CLOSY (EMBL:M60920) Clostridium symbiosum pyruvate, phosphate dikinase (EC 2.7.9.1) (pyruvate,orthophosphate dikinase) PpdK, 873 aa; fasta scores: opt: 1765 z-score: 1941.1 E(): ; 51.7% identity in 899 aa overlap. Contains Pfam match to entry PF01326 PPDK_N_term, Pyruvate phosphate dikinase, PEP/pyruvate binding domain, two Pfam matches to PF00391 PEP-utilizers, PEP-utilizing enzymes and match to Prosite entry PS00370 PEP-utilizing enzymes phosphorylation site signature 3 4 19 14 [Reporter]SCO6991 (9K9)_PCR/7464 [Gene]SCO6991/6803 NC_003888 SC8F11.17, unknown, len: 68 aa. 3 4 19 13 [Reporter]SCO7365 (10G9)_PCR/7463 [Gene]SCO7365/6802 NC_003888 SC9H11.19, unknown, len: 107 aa. 3 3 2 15 [Reporter]SCO4404 (7K18)_PCR/5076 [Gene]SCO4404/4629 NC_003888 SC6F11.02c, probable ABC transport system integral membrane protein, len: 262 aa; similar to TR:BAB07697 (EMBL:AP001520) Bacillus halodurans ABC transporter (permease) BH3978, 249 aa; fasta scores: opt: 669 z-score: 757.4 E(): 0; 41.7% identity in 247 aa overlap. Contains Pfam match to entry PF01061 ABC2_membrane, ABC-2 type transporter and possible hydrophobic membrane spanning regions 3 4 19 12 [Reporter]SCO2556 (11C9)_PCR/7462 [Gene]SCO2556/6801 NC_003888 SCC77.23, unknown, len: 247 aa. Similar to Mycobacterium tuberculosis TR:O07720(EMBL:Z97193) hypothetical 26.9 KD protein (250 aa), fasta scores opt: 618 z-score: 666.7 E(): 9.6e-30 45.0% identity in 238 aa overlap 3 3 2 14 [Reporter]SCO6867 (8G18)_PCR/5075 [Gene]SCO6867/4628 NC_003888 SC7F9.19c, unknown, len: 152 aa. Similar to the N-terminus of Streptomyces coelicolor TR:Q9X896 (EMBL:AL049645) hypothetical 26.6 KD protein, SCE2.01 (254 aa), fasta scores opt: 203 z-score: 219.9 E(): 7.7e-05 42.0% identity in 81 aa overlap. 3 4 19 11 [Reporter]SCO3949 (12O5)_PCR/7461 [Gene]SCO3949/6800 NC_003888 SCD78.16, possible secreted peptidase, len: 349 aa; similar to conserved hypotheticals including YEBA_ECOLI Hypothetical 46.7 kDa protein from Escherichia coli (419 aa), fasta scores; opt: 279 z-score: 336.7, E(): 1.7e-11, (28.4% identity in 341 aa overlap). Also similar to (TR:O54308 (EMBL:U50357) zoocin A endopeptidase from Streptococcus zooepidemicus fasta scores; opt: 276, z-score: 307.3, E(): 7.7e-10, (37.2% identity in 148 aa overlap) and LSTP_STAST lysostaphin precursor from Staphylococcus staphylolyticus fasta score; opt: 270, z-score: 249.6, E(): 1.3e-06, (42.7% identity in 110 aa overlap). Probable N-terminal signal sequence. Probable coiled-coils from 42 to 97 (56 residues) Max score: 1.643 (probability 1.00) and from 125 to 193 (69 residues) Max score: 1.653 (probability 1.00) 3 3 2 12 [Reporter]SCO2331 (10O14)_PCR/5073 [Gene]SCO2331/4627 NC_003888 SCC53.22c, possible MarR-family transcriptional regulator, len: 137 aa; similar to many e.g. SW:Q10810 (YX18_MYCTU) hypothetical transcriptional regulator CY274.18 from Mycobacterium tuberculosis (139 aa) fasta scores; opt: 145, z-score: 193.6, E(): 0.0026, 28.9% identity in 128 aa overlap and SW:P42195 (PECS_ERWCH) regulatory protein PecS from Erwinia chrysanthemi (166 aa) fasta scores; opt: 109, z-score: 148.4, E(): 0.86, 25.6% identity in 129 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family. 3 3 2 11 [Reporter]SCO4498 (11K14)_PCR/5072 [Gene]SCO4498/4626 NC_003888 SCD35.05, probable proton transport protein, len: 440 aa; similar to TR:Q54104 (EMBL:X92556) Saccharopolyspora erythraea similar to GltP glutamate transporters HgtA, 449 aa; fasta scores: opt: 1339 z-score: 1482.7 E(): 0; 50.2% identity in 422 aa overlap and to SW:GLTP_BACSU (EMBL:U15147) Bacillus subtilis proton glutamate symport protein (glutamate-aspartate carrier protein) GltP, 414 aa; fasta scores: opt: 684 z-score: 760.6 E(): 0; 28.9% identity in 409 aa overlap. Contains Pfam match to entry PF00375 SDF, Sodium:dicarboxylate symporter family and possible hydrophobic membrane spanning regions 3 3 2 10 [Reporter]SCO4246 (12G14)_PCR/5071 [Gene]SCO4246/4625 NC_003888 SCD8A.19c, unknown, len: 643 aa 3 3 2 9 [Reporter]SCO0932 (13C14)_PCR/5070 [Gene]SCO0932/4624 NC_003888 SCM10.20, possible integral membrane protein, len: 254 aa. Contains possible N-terminal region signal peptide sequence 3 3 2 8 [Reporter]SCO0121 (14O10)_PCR/5069 [Gene]SCO0121/4623 NC_003888 SCJ21.02c, probable ABC transport system ATP-binding protein, len: 204 aa; similar to many e.g. NUC_ECOLI high-affinity zinc uptake system ATP-binding protein (251 aa), fasta scores; opt: 327 z-score: 404.3 E(): 3.6e-15, 35.2% identity in 179 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and Pfam match to entry PF00005 ABC_tran, ABC transporter 3 3 2 7 [Reporter]SCO3432 (15K10)_PCR/5068 [Gene]SCO3432/4622 NC_003888 SCE9.39c, hypothetical protein, len: 418 aa; unknown function, C-terminal end similar to TR:O07428 (EMBL:Z97050) Mycobacterium tuberculosis hypothetical protein (249 aa), fasta scores; opt: 231 z-score: 252.5 E(): 9.7e-07, 30.4% identity in 270 aa overlap. Contains probable coiled-coil from 254 to 283 (30 residues) (Max score: 1.418, probability 0.78) 2 2 8 16 [Reporter]SCO0315 (7F3)_PCR/2689 [Gene]SCO0315/2449 NC_003888 SC5G9.24c, probable decarboxylase, len: 452 aa; similar to many e.g. SW:DCDA_BACSU (EMBL:L09228), LysA, Bacillus subtilis diaminopimelate decarboxylase (441 aa), fasta scores; opt: 484 z-score: 542.1 E(): 7.9e-23, 31.6% identity in 389 aa overlap. Similar to TR:Q9ZBH5 (EMBL:AL035206), DcdA, Streptomyces coelicolor diaminopimelate decarboxylase (440 aa), fasta scores; opt: 400 z-score: 384.5 E(): 3.6e-16, 30.2% identity in 427 aa overlap. Contains 2 Pfam matches to entry PF00278 Orn_DAP_Arg_deC, Pyridoxal-dependent decarboxylase, PS00879 Orn/DAP/Arg decarboxylases family 2 signature 2 and PS00878 Orn/DAP/Arg decarboxylases family 2 pyridoxal-P attachment site 3 3 2 6 [Reporter]SCO4662 (16G10)_PCR/5067 [Gene]SCO4662/4621 NC_003888 SCD40A.08, tuf1, elongation factor TU-1, len: 397 aa; identical to previously sequenced SW:EFT1_STRCO (EMBL:X77039) Streptomyces coelicolor elongation factor TU-1 (EF-TU-1), Tuf1, 397 aa. Contains Pfam match to entry PF00009 GTP_EFTU, Elongation factor Tu family and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00301 GTP-binding elongation factors signature 2 2 8 15 [Reporter]SCO2157 (8B3)_PCR/2688 [Gene]SCO2157/2448 NC_003888 SC6G10.30, probable aminotransferase, len: 460 aa; similar to many NifS-like proteins e.g. SW:NIFS_RHOSH NifS protein from Rhodobacter sphaeroides (387 aa) fasta scores; opt: 702, z-score: 760.5, E(): 0, (37.4% identity in 390 aa overlap). Contains Pfam matches to entry PF00266 aminotran_5, Aminotransferases class-V and entry PF01206 UPF0033, Uncharacterized protein family UPF0033. 3 3 2 5 [Reporter]SCO3824 (17C10)_PCR/5066 [Gene]SCO3824/4620 NC_003888 SCGD3.25, probable ABC-transporter ATP-binding component, len: 342 aa; may be involved in antibiotic resistance by acting as an efflux pump, highly similar to to many ABC-tranporter ATP-binding components (especially those involved in antibiotic transport) e.g. TR:O86916 (EMBL:Y15759) Streptomyces rochei ABC-tranporter which induces multidrug resistance (363 aa), fasta scores; opt: 1635 z-score: 1717.7 E(): 0, 77.6% identity in 340 aa overlap. Similar to many from S.coelicolor e.g. (EMBL:AL049841), SCE9.25c, S.coelicolor probable ABC transporter ATP-binding component (315 aa) (53.2% identity in 310 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, score 192.70, PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 14 8 [Reporter]SCO1729 (14N24)_PCR/797 [Gene]SCO1729/729 NC_003888 SCI11.18, hypothetical protein, len: 308 aa; unknown function, N-terminal half weakly similar to parts of other hypothetical proteins e.g. TR:O32075 (EMBL:Z99119) Bacillus subtilis hypothetical protein (173 aa), fasta scores; opt: 148 z-score: 187.8 E(): 0.004, 28.0% identity in 168 aa overlap. Weakly similar to TR:Q9ZBG7 (EMBL:AL035206) S.coelicolor hypothetical protein (319 aa) (30.1% identity in 272 aa overlap). Contains weak Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 2 2 8 14 [Reporter]SCO6581 (8N23)_PCR/2687 [Gene]SCO6581/2447 NC_003888 SC8A6.02c, probable transmembrane transport protein, len: 476 aa; some similarity to e.g. TR:Q51330 (EMBL:U4007 5) oxalate:formate antiport protein from Oxalobacter formig enes (418 aa), fasta scores; opt: 226 z-score: 230.1 E(): 1 .4e-05, 24.1% identity in 436 aa overlap 2 1 14 7 [Reporter]SCO1193 (15J24)_PCR/796 [Gene]SCO1193/728 NC_003888 SCG11A.24c, probable tetR-family transcriptional regulator, len: 189 aa; similar to TR:CAB56380 (EMBL:AL118515) Streptomyces coelicolor probable tetR-family transcriptional regulator SCD17.03, 226 aa; fasta scores: opt: 233 z-score: 295.6 E(): 4.3e-09; 35.2% identity in 176 aa overlap. Contains match to Pfam entry PF00440 tetR, Bacterial regulatory proteins, tetR family 2 2 8 13 [Reporter]SCO1504 (9J23)_PCR/2686 [Gene]SCO1504/2446 NC_003888 SC9C5.28, putative regulator, len: 750 aa; similar to TR:O53720 (EMBL:AL021931) Mycobacterium tuberculosis transcriptional regulatory protein MTV036.21, 1085 aa; fasta scores: opt: 975 z-score: 1090.3 E(): 0; 34.3% identity in 724 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 14 6 [Reporter]SCO1587 (16F24)_PCR/795 [Gene]SCO1587/727 NC_003888 SCI35.09c, possible transcriptional regulator, len: 480 aa; similar to hypothetical proteins e.g. TR:P96681 (EMBL:AB001488) YdfD (B. subtilis) (482 aa), fasta scores; opt: 535 z-score: 419.1 E(): 4.2e-16, 26.1% identity in 471 aa overlap and YRDX_RHOSH hypothetical transcriptional regulator (456 aa), fasta scores; opt: 299 z-score: 407.0 E(): 2e-15, 27.2% identity in 464 aa overlap. Contains probable helix-turn-helix motif at aa 17-38 (Score 1327, +3.71 SD) and Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family, score 41.30, E-value 3.4e-11 2 2 8 12 [Reporter]SCO2725 (10F23)_PCR/2685 [Gene]SCO2725/2445 NC_003888 SCC46.10, possible lipoprotein, len: 285 aa; identical to previously sequenced TR:Q53936 (EMBL:L48550) Streptomyces coelicolor unknown protein downstream of msdA. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal signal peptide sequence 2 1 14 5 [Reporter]SCO3310 (17B24)_PCR/794 [Gene]SCO3310/726 NC_003888 SCE68.08, hypothetical protein, len: 84 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 2 2 8 11 [Reporter]SCO4095 (11B23)_PCR/2684 [Gene]SCO4095/2444 NC_003888 SCD25.31, possible lipoprotein, len: 185 aa. Contains a possible N-terminal signal sequence and a Prosite hit to a PS00013 Prokaryotic membrane lipoprotein lipid attachment site. The protein product is also predicted to be rich in Pro/Ala/Gly. 2 1 14 4 [Reporter]SCP1.200c (18N20)_PCR/793 [Gene]SCP1.200c/725 NC_003888 SCP1.200c, possible secreted protein, len: 166aa; similar to TR:O87846 (EMBL:AL031013) putative secreted protein from Streptomyces coelicolor (168 aa) fasta scores; opt: 313, z-score: 373.4 ,E(): 2.5e-13, 40.3% identity in 154 aa overlap. Contains a possible cleavable N-terminal signal sequence. 2 2 8 10 [Reporter]SCO2931 (12N19)_PCR/2683 [Gene]SCO2931/2443 NC_003888 SCE19A.31, probable ABC transporter ATP-binding protein, len: 424 aa; similar to many, including components of transporters involved in osmoregulation e.g. SW:PROV_BACSU (EMBL:U38418), proV, Bacillus subtilis glycine betaine/L-proline transport ATP-binding protein (404 aa), fasta scores; opt: 997 z-score: 1092.8 E(): 0, 45.7% identity in 359 aa overlap. Similar to SC9B1.09 (EMBL:AL049727) S.coelicolor ABC transporter ATP-binding subunit (386 aa) (49.0% identity in 365 aa overlap). Contains an approx. 20 aa N-terminal extension absent similar proteins. May be part of a multicomponent transporter which includes SCE19A.30, SCE19A.31, SCE19A.32 and SCE19A.33. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 14 3 [Reporter]SCO6084 (19J20)_PCR/792 [Gene]SCO6084/724 NC_003888 SCBAC1A6.08, possible DNA polymerase, len: 244 aa; similar to SW:DP3E_ECOLI (EMBL:X04027) Escherichia coli DNA polymerase III, epsilon chain (EC 2.7.7.7) DnaQ, 243 aa; fasta scores: opt: 137 z-score: 167.9 E(): 0.089; 26.2% identity in 206 aa overlap and to TR:Q9L275 (EMBL:AL137778) Streptomyces coelicolor putative DNA polymerase III SCL2.24, 241 aa; fasta scores: opt: 580 z-score: 680.3 E(): 2.6e-30; 42.7% identity in 234 aa overlap. Contains Pfam match to entry PF00929 Exonuclease, Exonuclease 2 2 8 9 [Reporter]SCO2889 (13J19)_PCR/2682 [Gene]SCO2889/2442 NC_003888 SCE6.26c, possible sugar transport protein, len: 504 aa. Similar to several Eukaryotic and Prokaryotic transport proteins including: Schizosaccharomyces pombe (Fission yeast) SW:ITR2_SCHPO(EMBL:Z95334) myo-inositol transporter 2 (557 aa), fasta scores opt: 422 z-score: 488.9 E(): 8.4e-20 27.6% identity in 467 aa overlap and Bacillus subtilis SW:YYAJ_BACSU(EMBL:D26185) hypothetical metabolite transport protein (451 aa), fasta scores opt: 420 z-score: 487.9 E(): 9.6e-20 27.7% identity in 451 aa overlap. Contains a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and multiple possible membrane spanning hydrophobic domains. 2 1 14 2 [Reporter]SCO0981 (20B20)_PCR/791 [Gene]SCO0981/723 NC_003888 SCBAC19F3.08c, possible DNA-binding protein, len: 489aa: similar to many eg. TR:AAK44705 (EMBL:AE006950) putative DNA-binding protein MT0481 from Mycobacterium tuberculosis (474 aa) fasta scores; opt: 1753, Z-score: 1963.4, 56.780% identity (58.009% ungapped) in 472 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 2 2 8 8 [Reporter]SCO3899 (14F19)_PCR/2681 [Gene]SCO3899/2441 NC_003888 SCH24.21c, conserved hypothetical protein, len: 360 aa; unknown function, similar to e.g. TR:P71703 (EMBL:Z80775) Mycobacterium tuberculosis hypothetical protein (367 aa), fasta scores; opt: 1790 z-score: 2136.2 E(): 0, 78.5% identity in 353 aa overlap. C-terminal half shows weak similarity with a region of SW:INO1_CANAL (EMBL:L22737), Ino1, Candida albicans myo-inositol-1-phosphate synthase (520 aa) (26.3% identity in 251 aa overlap) and with TR:O69948 (EMBL:AL023862) S.coelicolor probable secreted protein (417 aa) (25.2% identity in 226 aa overlap) 2 1 13 22 [Reporter]SCO1008 (1B16)_PCR/789 [Gene]SCO1008/722 NC_003888 2SCG2.21, hypothetical protein, len: 111 aa; similar to the plasmid SCP1 protein TR:Q52517 (EMBL:M18263) Streptomyces coelicolor hypothetical 12.1 kDa protein MmyJ, 111 aa; fasta scores: opt: 246 z-score: 332.3 E(): 5.5e-11; 43.8% identity in 89 aa overlap 2 2 8 7 [Reporter]SCO1909 (15B19)_PCR/2680 [Gene]SCO1909/2440 NC_003888 SCI7.27c, unknown, len: 235aa; 2 1 13 21 [Reporter]SCO1473 (2N12)_PCR/788 [Gene]SCO1473/721 NC_003888 SCL6.30c, fmt, methionyl-tRNA formyltransferase, len: 310 aa; highly similar to SW:FMT_MYCTU (EMBL:Z80108) Mycobacterium tuberculosis methionyl-tRNA formyltransferase (EC 2.1.2.9) Fmt, 312 aa; fasta scores: opt: 1119 z-score: 1259.3 E(): 0; 56.3% identity in 311 aa overlap and to SW:FMT_ECOLI (EMBL:X63666;) Escherichia coli methionyl-tRNA formyltransferase (EC 2.1.2.9) Fmt, 314 aa; fasta scores: opt: 733 z-score: 827.4 E(): 0; 38.9% identity in 311 aa overlap. Contains Pfam match to entry PF00551 formyl_transf, Formyl transferase 2 1 13 20 [Reporter]SCO5924 (3J12)_PCR/787 [Gene]SCO5924/720 NC_003888 SC10A5.29c, probable integral membrane protein, len: 589 aa; similar to many permeases eg. TR:Q44123 (EMBL:U050 42) iron utilisation protein AFUB from Actinobacillus pleur opneumoniae (687 aa), fasta scores; opt: 564 z-score: 426.3 E(): 1.5e-16, 25.0% identity in 547 aa overlap 3 3 2 4 [Reporter]SCO0922 (18O6)_PCR/5065 [Gene]SCO0922/4619 NC_003888 SCM10.10c, probable reductase iron-sulfur protein, len: 248 aa; similar to SW:FRDB_MYCTU (EMBL:Z74020) Mycobacterium tuberculosis fumarate reductase iron-sulfur protein (EC 1.3.99.1) FrdB, 247 aa; fasta scores: opt: 288 z-score: 321.7 E(): 1.6e-10; 28.9% identity in 239 aa overlap. Contains Pfam matches to entry PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains and entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains and to Prosite entries PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature and PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature 3 3 2 3 [Reporter]SCO1869 (19K6)_PCR/5064 [Gene]SCO1869/4618 NC_003888 SCI39.16c, conserved hypothetical protein, len: 237 aa; similar to TR:O68911 (EMBL:AF058302) Streptomyces roseofulvus FrnE protein, 216 aa; fasta scores: opt: 528 Z-score: 605.5 bits: 119.3 E(): 3.9e-26; 46.919% identity in 211 aa overlap and to TR:Q9ZA40 (EMBL:AJ011500) Streptomyces violaceoruber putative disulphide bond-forming protein Gra-ORF19, 220 aa; fasta scores: opt: 316 Z-score: 366.6 bits: 75.1 E(): 7.9e-13; 37.104% identity in 221 aa overlap 3 3 2 2 [Reporter]SCP1.280 (20C6)_PCR/5063 [Gene]SCP1.280/4617 NC_003888 SCP1.280, unknown, len: 537aa; weakly similar to TR:Q9L0Y7 (EMBL:AL159139) hypothetical protein from Streptomyces coelicolor (475 aa) fasta scores; opt: 141, z-score: 161.2, E(): 0.16, 26.0% identity in 407 aa overlap. 3 3 1 22 [Reporter]SCO5187 (1C2)_PCR/5061 [Gene]SCO5187/4616 NC_003888 2SC3B6.11c, possible glutaredoxin-like protein, len: 80 aa; similar to SW:NRDH_LACLC (EMBL:X92690) Lactococcus lactis glutaredoxin-like protein NrdH, 72 aa; fasta scores: opt: 119 z-score: 176.0 E(): 0.024; 35.6% identity in 59 aa overlap. Contains match to Prosite entry PS00194 Thioredoxin family active site 3 3 1 19 [Reporter]SCO7395 (3G22)_PCR/5058 [Gene]SCO7395/4615 NC_003888 SC10G8.23c, possible membrane transport protein, len: 521 aa. Highly similar in several transport proteins involved in osmoregulation e.g. Escherichia coli SW:PROP_ECOLI(EMBL:M83089) proline/betaine transporter (proline porter II), ProP (500 aa), fasta scores opt: 1557 z-score: 1688.1 E():0 47.9% identity in 466 aa overlap. Contains Prosite hits to PS00217 Sugar transport proteins signature 2 and PS00216 Sugar transport proteins signature 1, as well as a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter. Also contains multiple possible membrane spanning hydrophobic domains. 3 3 1 18 [Reporter]SCO6185 (4C22)_PCR/5057 [Gene]SCO6185/4614 NC_003888 SC2G5.06, possible transferase, len: 402aa; similar to many egs. SW:YV12_MYCTU hypothetical protein from Mycobacterium tuberculosis (480 aa) fasta scores; opt: 652, z-score: 737.4, E(): 0, (34.6% identity in 408 aa overlap) and TR:O84909 (EMBL:AF010182) glycosyl transferase, WbpY from Pseudomonas aeruginosa (371 aa) fasta scores; opt: 298, z-score: 340.5, E(): 1.1e-11, (29.4% identity in 326 aa overlap). Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1, score 111.00, E-value 1.2e-30. 3 3 1 17 [Reporter]SCO6709 (5O18)_PCR/5056 [Gene]SCO6709/4613 NC_003888 SC4C6.19, conserved hypothetical protein, len: 341 aa; similar to hypothetical proteins from Mycobacterium tuberculosis, Mycobacterium leprae and Bacillus subtilis e.g. TR:O69697 (EMBL:AL02212) Mycobacterium tuberculosis hypothetical protein (346 aa), fasta scores; opt: 1313 z-score: 1504.6 E(): 0, 59.2% identity in 336 aa overlap. Similarity is confined to the N-terminal end in cases where the similar protein is longer then this one e.g. TR:O05865 (EMBL:Z95209) Mycobacterium tuberculosis hypothetical protein (759 aa) (28.3% identity in 336 aa overlap). Also similar to SC1E6.07 (EMBL:AL033505) S.coelicolor hypothetical protein (319 aa) (35.8% identity in 288 aa overlap) 3 3 1 16 [Reporter]SCO2165 (6K18)_PCR/5055 [Gene]SCO2165/4612 NC_003888 SC5F7.36c, probable two component system response regulator, len: 227 aa; similar to many others from Streptomyces coelicolor egs. TR:O69816 (EMBL:AL023496) proposed two component regulator (224 aa) fasta scores; opt: 753, z-score: 882.2, E(): 0, (57.4% identity in 223 aa overlap) and TR:Q53894 (EMBL:U51332) AbsA2 two component response regulator of antibiotic synthesis (222 aa) fasta scores; opt: 564, z-score: 663.8, E(): 1.2e-29, (44.6% identity in 222 aa overlap). Contains Prosite match to PS00622 Bacterial regulatory proteins, luxR family signature and Pfam matches to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and to entry PF00072 response_reg, Response regulator receiver domain.Contains helix-turn-helix motif (+2.94 SD) 178-199aa. 2 2 8 6 [Reporter]SCO0419 (16N15)_PCR/2679 [Gene]SCO0419/2439 NC_003888 SCF51.18, possible zinc-binding oxidoreductase, len: 329 aa; similar to SW:QOR_PSEAE (EMBL:X85015) Pseudomonas aeruginosa quinone oxidoreductase (EC 1.6.5.5) (NADPH:quinone reductase) qor, 325 aa; fasta scores: opt: 420 z-score: 452.5 E(): 7.8e-18; 32.8% identity in 332 aa overlap and to TR:CAB45348 (EMBL:AL079345) Streptomyces coelicolor putative dehydrogenase SCE68.12c, 318 aa; fasta scores: opt: 535 z-score: 574.1 E(): 1.3e-24; 38.6% identity in 332 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases 3 3 1 15 [Reporter]SCO2674 (7G18)_PCR/5054 [Gene]SCO2674/4611 NC_003888 SC6D10.17, probable ABC transporter ATP-binding protein, len: 319 aa; similar to various ABC transporter, ATP-binding proteins, e.g. TR:O28803 (EMBL:AE001001) Archaeoglobus fulgidus ABC transporter ATP-binding protein AF1469, 226 aa; fasta scores: opt: 631 z-score: 717.1 E(): 1.6e-32; 45.7% identity in 219 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 2 2 8 5 [Reporter]SCO3665 (17J15)_PCR/2678 [Gene]SCO3665/2438 NC_003888 SCH44.05, possible regulatory protein, len: 327 aa; similar to TR:Q56084 (EMBL:D63904) Streptomyces thermoviolaceus ESA36 (erythropoiesis-stimulating activity), 344 aa; fasta scores: opt: 270 z-score: 315.4 E(): 4.2e-10; 25.7% identity in 296 aa overlap and als to Streptomyces colicolor SCH44.04 336 aa; fasta scores: opt: 973 z-score: 954.8 E(): 0; 48.0% identity in 325 aa overlap and SCH44.06c 394 aa; fasta scores: opt: 849 z-score: 833.2 E(): 0; 45.2% identity in 323 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and possible helix-turn-helix motif at residues 278..299 (+5.13 SD) 3 3 1 14 [Reporter]SCO6863 (8C18)_PCR/5053 [Gene]SCO6863/4610 NC_003888 SC7F9.15c, unknown, len: 209 aa. 2 1 13 19 [Reporter]SCO6028 (4F12)_PCR/786 [Gene]SCO6028/719 NC_003888 SC1C3.16c, possible ribonuclease, len: 394 aa; similar to hypothetical proteins e.g. M. tuberculosis TR:O07183 (EMBL:Z96072) MTCY05A6.02 (438 aa), fasta scores; opt: 1338 z-score: 1167.9 E(): 0, 51.9% identity in 405 aa overlap 2 2 8 4 [Reporter]SCO3328 (18F15)_PCR/2677 [Gene]SCO3328/2437 NC_003888 SCE68.26c, hypothetical protein, len: 70 aa; similar to TR:Q49828 (EMBL:U00018) Mycobacterium leprae hypothetical protein (86 aa), fasta scores; opt: 269 z-score: 380.5 E(): 7.2e-14, 68.3% identity in 63 aa overlap. Weakly similar to putative excisionases e.g. TR:Q54076 (EMBL:L11597), xis, Saccharopolyspora erythraea excisionase (76 aa) (34.0% identity in 47 aa overlap) and SCE29.20c (EMBL:AL035707) S.coelicolor probable excisionase (66 aa) (33.3% identity in 48 aa overlap). Contains probable helix-turn-helix motif at aa 16-37 (Score 1251, +3.45 SD) 2 1 13 18 [Reporter]SCO5459 (5B12)_PCR/785 [Gene]SCO5459/718 NC_003888 SC3D11.16, probable enoyl-coA hydratase, len: 255aa; similar to many eg. SW:P52046 (CRT_CLOAB) 3-hydroxybutyryl-coa dehydratase from Clostridium acetobutylicum (261 aa) fasta scores; opt: 637, z-score: 725.4, E(): 5.4e-33, 43.4% identity in 249 aa overlap. Contains Pfam match to entry PF00378 ECH, Enoyl-CoA hydratase/isomerase family and Prosite match to PS00166 Enoyl-CoA hydratase/isomerase signature. 2 2 8 3 [Reporter]SCP1.228c (19B15)_PCR/2676 [Gene]SCP1.228c/2436 NC_003888 SCP1.228c, mmyF, possible oxidoreductase, len: 174aa; previously sequenced and annotated as TR:Q9JN75 (EMBL:AJ276673). Similar to many oxidoreductases eg. N-terminal region of SW:P54990 (NTAB_CHEHE) nitrilotriacetate monooxygenase component B (EC 1.14.13.-) from Chelatobacter heintzii (322 aa) fasta scores; opt: 419, z-score: 522.1, E(): 1.3e-21, 41.4% identity in 169 aa overlap and SW:Q02058 (DIM6_STRCO) actinorhodin polyketide dimerase from Streptomyces coelicolor (177 aa) fasta scores; opt: 295, z-score: 373.9, E(): 2.3e-13, 31.0% identity in 158 aa overlap. Contains Pfam match to entry PF01613 Flavin_Reduct, Flavin reductase like domain. 2 1 13 17 [Reporter]SCO7451 (6N8)_PCR/784 [Gene]SCO7451/717 NC_003888 SC5C11.08, unknown, len: 398 aa. Highly similar to several proteins of undefined function associated with polyketide biosynthesis including: Streptomyces coelicolor SW:WH42_STRCO(EMBL:X55942) 42.8 KD protein in whiE locus (WhiE ORF I) SC6G9.13 (397 aa), fasta scores opt: 1392 z-score: 1637.1 E():0 56.1% identity in 401 aa overlap and Streptomyces cyaneus (Streptomyces curacoi) SW:CURD_STRCN(EMBL:X62518) polyketide synthase, CurD (367 aa), fasta score opt: 1451 z-score: 1706.9 E():0 62.0% identity in 368 aa overlap. 2 2 8 2 [Reporter]SCO1567 (20J11)_PCR/2675 [Gene]SCO1567/2435 NC_003888 SCL24.03c, possible transmembrane-transport protein, len: 510 aa; similar to TR:O33968 (EMBL:U80063) Streptomyces cinnamoneus putative transmembrane, proton-dependent transport protein, 519 aa; fasta scores: opt: 2339 z-score: 2439.5 E(): 0; 72.7% identity in 510 aa overlap and to SW:QACA_STAAU (EMBL:X56628) Staphylococcus aureus antiseptic resistance protein QacA, 514 aa; fasta scores: opt: 1116 z-score: 1166.8 E(): 0; 36.6% identity in 503 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 13 16 [Reporter]SCO6767 (7J8)_PCR/783 [Gene]SCO6767/716 NC_003888 SC6A5.16, GcpE protein homolog, len: 384 aa; similar to many of undefined function e.g. TR:O33350 (EMBL:AL008883) hypothetical protein from Mycobacterium tuberculosis (387 aa) fasta scores; opt: 1965, z-score: 2178.9, E(): 0, (78.2% identity in 385 aa overlap). Similar to SW:P27433 (GCPE_ECOLI) essential protein from Escherichia coli. 2 2 7 22 [Reporter]SCO4222 (1J7)_PCR/2673 [Gene]SCO4222/2434 NC_003888 2SCD46.36, conserved hypothetical protein, len: 126aa; similar to others from actinomycetes eg. TR:O06633 (EMBL:Z95618) hypothetical protein from Mycobacterium tuberculosis (115 aa) fasta scores; opt: 256, z-score: 332.9, E(): 4.5e-11, 36.1% identity in 119 aa overlap. 2 1 13 15 [Reporter]SCO5325 (8F8)_PCR/782 [Gene]SCO5325/715 NC_003888 SC6G9.08c, hypothetical protein, len: 106 aa; unknown function, improbable CD suggested by GC frameplot. C-terminus is proline-rich and overlaps with hypothetical CDS on opposite strand 2 2 7 21 [Reporter]SCO5424 (2F7)_PCR/2672 [Gene]SCO5424/2433 NC_003888 SC8F4.28c, ackA, acetate kinase, len: 407 aa; strongly similar to many e.g. SW:P77845 (ACKA_CORGL) AckA, acetate kinase from Corynebacterium glutamicum (Brevibacterium flavum) (397 aa) fasta scores; opt: 1333, z-score: 1504.1, E(): 0, 52.8% identity in 388 aa overlap. Contains Pfam match to entry PF00871 Acetate_kinase, Acetokinase family and Prosite match to PS01075 Acetate and butyrate kinases family signature 1. 2 1 13 14 [Reporter]SCO7749 (9B8)_PCR/781 [Gene]SCO7749/714 NC_003888 SC8D11.40, unknown, len: 364 aa 2 2 7 19 [Reporter]SCO6511 (4N3)_PCR/2670 [Gene]SCO6511/2432 NC_003888 SC1E6.20c, unknown, len: 501 aa; similar to two hypothetical proteins from Rhodococcus erythropolis: TR:P72262 (EMBL:Z82004) ORF8 (487 aa), fasta scores; opt: 1098 z-score: 762.9 E(): 0, 45.5% identity in 488 aa overlap, and TR:P72269 (EMBL:Z82005) ORF8 (487 aa), fasta scores; opt: 1060 z-score: 717.1 E(): 1.2e-32, 41.7% identity in 487 aa overlap. Contains Pfam match to entry PF00781 DAGKc, Diacylglycerol kinase catalytic domain (presumed), score 31.00, E-value 1.3e-05 2 1 13 13 [Reporter]SCO2782 (10N4)_PCR/780 [Gene]SCO2782/713 NC_003888 SCC105.13, probable pyridoxal-dependent decarboxylase, len: 480 aa; similar to SW:RHSB_RHIME (EMBL:AF110737) Rhizobium meliloti L-2,4-diaminobutyrate decarboxylase (EC 4.1.1.-) RhsB, 495 aa; fasta scores: opt: 1111 z-score: 1326.2 E(): 0; 41.9% identity in 458 aa overlap. Contains Pfam match to entry PF00282 pyridoxal_deC, Pyridoxal-dependent decarboxylase conserved domain 2 2 7 18 [Reporter]SCO5886 (5J3)_PCR/2669 [Gene]SCO5886/2431 NC_003888 SC3F7.06c, redR, probable 3-oxoacyl-[acyl-carrier-protein] synthase II, len: 407 aa; similar to many eg. FABF_ECOLI P39435 3-oxoacyl-[acyl-carrier-protein] synthase II (EC 2.3.1.41) (beta-ketoacyl-acp synthase II) (412 aa), fasta scores; opt: 1054 z-score: 1061.9 E(): 0, 42.0% identity in 412 aa overlap. Also some similarity to SC3F7.12 from aa 800 to 1210 (E(): 3.3e-10, 32.2% identity in 432 aa overlap). Contains Pfam match to entry ketoacyl-synt PF00109, Beta-ketoacyl synthases, score 243.99 2 1 13 12 [Reporter]SCO2372 (11J4)_PCR/779 [Gene]SCO2372/712 NC_003888 SCC8A.30c, possible small hydrophobic protein, len: 102 aa; similar to TR:O86580 (EMBL:AL031184) small hydrophobic protein from Streptomyces coelicolor (97 aa) fasta scores; opt: 119, z-score: 161.8, E(): 0.15, 33.3% identity in 81 aa overlap. Contains possible membrane-spanning hydrophobic region. 2 2 7 17 [Reporter]SCO6639 (6F3)_PCR/2668 [Gene]SCO6639/2430 NC_003888 SC4G2.13, probable ATP/GTP binding protein, len: 449 aa; similar to TR:O26411 (EMBL:AE000816) hypothetical protein MTH311 (Methanobacterium thermoautotrophicum) (411 aa), fasta scores; opt: 596 z-score: 528.7 E(): 3.3e-22, 30.5% identity in 393 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 13 11 [Reporter]SCO4789 (12F4)_PCR/778 [Gene]SCO4789/711 NC_003888 SCD63.21, possible integral membrane protein, len: 171 aa. Contains possible hydrophobic membrane spanning regions 2 1 13 10 [Reporter]SCO3197 (13B4)_PCR/777 [Gene]SCO3197/710 NC_003888 SCE22.14c, probable 1-phosphofructokinase, len: 315 aa; similar to SW:K1PF_RHOCA (EMBL:X53150) Rhodobacter capsulatus 1-phosphofructokinase (EC 2.7.1.56) (fructose 1-phosphate kinase) FruK, 316 aa; fasta scores: opt: 555 z-score: 576.5 E(): 1.2e-24; 37.8% identity in 315 aa overlap. Contains Pfam match to entry PF00294 pfkB, pfkB family carbohydrate kinase and match to Prosite entry PS00583 pfkB family of carbohydrate kinases signature 1 3 3 1 13 [Reporter]SCO5855 (9O14)_PCR/5052 [Gene]SCO5855/4609 NC_003888 SC9B10.22c, unknown, len: 352 aa; similar to hypothetical proteins from Mycobacteruim tuberculosis and M. leprae; YZ11_MYCTU Q10545 MTCY31.11c (253 aa), fasta scores; opt: 444 z-score: 396.4 E(): 6.4e-15, 35.0% identity in 246 aa overlap. Contains probable helix-turn-helix domain at aa positions 70-91 (Score 1098, +2.93 SD) 3 3 1 12 [Reporter]SCO2327 (10K14)_PCR/5051 [Gene]SCO2327/4608 NC_003888 SCC53.18c, unknown, len: 128aa; strongly similar to TR:CAB82885 (EMBL:AJ276673) from plasmid pSCP1 from Streptomyces coelicolor (128 aa) fasta scores; opt: 395, z-score: 534.6, E(): 2.6e-22, 51.6% identity in 128 aa overlap. 3 3 1 11 [Reporter]SCO4104 (11G14)_PCR/5050 [Gene]SCO4104/4607 NC_003888 SCD17.08c, possible integral membrane protein, len: 218 aa. Similar to several hypothetical membrane proteins e.g. Streptomyces coelicolor TR:O86576 (EMBL; AL031184) putative integral membrane protein SC2A11.15 (219 aa), fasta scores opt: 503 z-score: 576.2 E(): 9.7e-25 41.6% identity in 209 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 3 3 1 10 [Reporter]SCO4242 (12C14)_PCR/5049 [Gene]SCO4242/4606 NC_003888 SCD8A.15c, possible membrane protein, len: 468 aa. Contains possible hydrophobic membrane spanning regions 3 3 1 9 [Reporter]SCO3120 (13O10)_PCR/5048 [Gene]SCO3120/4605 NC_003888 SCE41.29c, unknown, len: 69 aa 3 3 1 8 [Reporter]SCO0940 (14K10)_PCR/5047 [Gene]SCO0940/4604 NC_003888 SCM10.28, possible marR-family regulatory protein, len: 157 aa; weakly similar to TR:O07458 (EMBL:U75363) Rhodopseudomonas palustris putative regulatory protein, 175 aa; fasta scores: opt: 150 z-score: 189.7 E(): 0.0036; 28.1% identity in 160 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 3 3 1 7 [Reporter]SCO5515 (15G10)_PCR/5046 [Gene]SCO5515/4603 NC_003888 SC8D9.27, serA, D-3-phosphoglycerate dehydrogenase, len: 529 aa; member of a family including egs. SW:SERA_MYCLE putative SerA, D-3-phosphoglycerate dehydrogenase from Mycobacterium leprae (528 aa) fasta scores; opt: 1889, z-score: 2032.0, E(): 0, (56.9% identity in 524 aa overlap) and SW:SERA_BACSU SerA, D-3-phosphoglycerate dehydrogenase from Bacillus subtilis (525 aa) fasta scores; opt: 1176, z-score: 1266.5, E(): 0, (39.7% identity in 529 aa overlap). Contains PS00065 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature, PS00670 D-isomer specific 2-hydroxyacid dehydrogenases signature 2 and Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases, score 256.90, E-value 7e-105. 2 2 7 16 [Reporter]SCO0311 (7B3)_PCR/2667 [Gene]SCO0311/2429 NC_003888 SC5G9.20, probable ligase, len: 518 aa; similar to domains within polyketide synthases e.g. to the N-terminus of TR:Q54297 (EMBL:X86780), RapA, Streptomyces hygroscopicus polyketide synthase (8563 aa), fasta scores; opt: 815 z-score: 894.4 E(): 0, 34.0% identity in 509 aa overlap and to SW:DHBE_BACSU (EMBL:U26444), DhbE, EntE, Bacillus subtilis 2,3-dihydroxybenzoate-AMP ligase (539 aa) (31.3% identity in 520 aa overlap). Also similar to others from S. coelicolor e.g. TR:Q9Z5A6 (EMBL:AL035478) S. coelicolor probable long-chain-fatty-acid-CoA ligase (541 aa) (35.9% identity in 537 aa overlap). Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and PS00455 Putative AMP-binding domain signature 3 3 1 6 [Reporter]SCO0887 (16C10)_PCR/5045 [Gene]SCO0887/4602 NC_003888 SCM1.20c, possible TetR-family transcriptional regulator, len: 234 aa. Similar to another Streptomyces coelicolor putative TetR-family transcriptional regulator TR:CAB58280 (EMBL:AL121849) (194 aa), fasta scores opt: 196 z-score: 233.7 E(): 1.1e-05 30.8% identity in 208 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with a putative helix-turn-helix motif situated between residues 61..82 (+3.80 SD). 2 2 7 15 [Reporter]SCO2129 (7N23)_PCR/2666 [Gene]SCO2129/2428 NC_003888 SC6G10.02c, hypothetical protein, len: 144 aa; similar to hypothetical proteins from Mycobacterium eg. TR:O53519 (EMBL:AL021957) hypothetical protein from Mycobacterium tuberculosis (144 aa) fasta scores; opt: 479, z-score: 620.2, E(): 3.1e-27, (49.0% identity in 143 aa overlap) 3 3 1 5 [Reporter]SCO4870 (17O6)_PCR/5044 [Gene]SCO4870/4601 NC_003888 SCK20.11c, probable monooxygenase, len: 483 aa; similar to TR:P95598 (EMBL:U56415) Corynebacterium equii rifampin monooxygenase Iri, 479 aa; fasta scores: opt: 691 z-score: 714.1 E(): 3.1e-32; 35.0% identity in 488 aa overlap and to TR:O86481 (EMBL:AJ007932) Streptomyces argillaceus oxygenase MtmOII, 531 aa; fasta scores: opt: 590 z-score: 609.9 E(): 1.9e-26; 33.3% identity in 520 aa overlap. Contains Pfam match to entry PF01494 FAD_binding_3, FAD binding domain and match PF01360 Monooxygenase, Monooxygenase 2 1 13 9 [Reporter]SCO2984 (13N24)_PCR/776 [Gene]SCO2984/709 NC_003888 SCE50.12, possible integral membrane protein, len: 443 aa. Contains possible hydrophobic membrane spanning regions and possible N-terminal region signal peptide sequence 2 2 7 14 [Reporter]SCO2416 (8J23)_PCR/2665 [Gene]SCO2416/2427 NC_003888 SC8A2.04c, possible secreted protein, len: 337 aa; similar to TR:O53970 (EMBL:AL022073) Mycobacterium tuberculosis hypothetical 44.4 kDa protein MTV051.07, 423 aa; fasta scores: opt: 596 z-score: 625.4 E(): 2.6e-27; 33.1% identity in 320 aa overlap. Contains Pfam match to entry PF02470 mce. Also contains possible N-terminal region signal peptide sequence 3 3 1 4 [Reporter]SCO2957 (18K6)_PCR/5043 [Gene]SCO2957/4600 NC_003888 SCE59.16c, unknown, len: 192 aa 2 1 13 8 [Reporter]SCO1573 (14J24)_PCR/775 [Gene]SCO1573/708 NC_003888 SCL24.09, possible oxidoreductase (putative membrane protein), len: 445 aa; similar to SW:XYLZ_PSEPU (EMBL:M64747) Pseudomonas putida toluate 1,2-dioxygenase subunit XylZ, 336 aa; fasta scores: opt: 236 z-score: 273.1 E(): 8.6e-08; 25.4% identity in 228 aa overlap. Contains Pfam match to entry PF00175 oxidored_fad, Oxidoreductase FAD/NAD-binding domain. Contains also possible hydrophobic membrane spanning regions 2 2 7 13 [Reporter]SCO6068 (9F23)_PCR/2664 [Gene]SCO6068/2426 NC_003888 SC9B1.15c, cvnB6, unknown, len: 119 aa; similar to other hypothetical proteins egs. two from Streptomyces coelicolor: TR:O86521 (EMBL:AL031124) (140 aa) fasta scores; opt: 331, z-score: 424.2, E(): 2.6e-16, (41.5% identity in 130 aa overlap) and TR:O86524 (EMBL:AL031124) (137 aa) fasta scores; opt: 325, z-score: 416.9, E(): 6.7e-16, (44.3% identity in 122 aa overlap), and TR:O50393 (EMBL:AL009198) from Mycobacterium tuberculosis (130 aa) fasta scores; opt: 282, z-score: 364.1, E(): 5.8e-13, (39.1% identity in 115 aa overlap). 2 1 13 7 [Reporter]SCO0672 (15F24)_PCR/774 [Gene]SCO0672/707 NC_003888 SCF91.32, possible anti-sigma factor antagonist, len: 183 aa. Similar in parts to many putative anti-sigma factor antagonists including: Streptomyces coelicolor TR:CAB44395 (EMBL:AL078610) SCH35.32C (113 aa), fasta scores opt: 199 z-score: 246.4 E(): 2.4e-06 33.9% identity in 112 aa overlap. Contains a Pfam match to entry PF01740 SpoIIAA. 2 2 7 12 [Reporter]SCO2721 (10B23)_PCR/2663 [Gene]SCO2721/2425 NC_003888 SCC46.06c, possible lipoprotein, len: 532 aa; similar to various hypothetical proteins, e.g. SW:YFBK_ECOLI (EMBL:AE000317) Escherichia coli hypothetical 63.6 kD protein in ElaD-NuoN intergenic region, YfbK, 575 aa; fasta scores: opt: 778 z-score: 815.2 E(): 0; 32.6% identity in 482 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site 2 1 13 6 [Reporter]SCO3149 (16B24)_PCR/773 [Gene]SCO3149/706 NC_003888 SCE66.28c, possible dimethyladenosine transferase, len: 286aa; similar to many eg. SW:P06992 (KSGA_ECOLI) dimethyladenosine transferase from Escherichia coli (273 aa) fasta scores; opt: 425, z-score: 481.5, E(): 2.4e-19, 32.8% identity in 262 aa overlap. Contains Pfam match to entry PF00398 RrnaAD, Ribosomal RNA adenine dimethylases and Prosite match to PS01131 Ribosomal RNA adenine dimethylases signature. 2 2 7 11 [Reporter]SCO4625 (11N19)_PCR/2662 [Gene]SCO4625/2424 NC_003888 SCD39.25c, hypothetical protein, len: 335 aa; similar to TR:Q54674 (EMBL:U23762) Streptomyces phaeochromogenes involved in intramycelial plasmid spread protein SpdB2, 371 aa; fasta scores: opt: 799 z-score: 782.1 E(): 0; 45.1% identity in 368 aa overlap. Contains colied-coil region at aprox . 184..262 aa (coinciding with a high hidropihilicity region in the protein) and possible N-terminal region signal peptide sequence 2 1 13 5 [Reporter]SCO1826 (17N20)_PCR/772 [Gene]SCO1826/705 NC_003888 SCI8.11, possible membrane protein, len: 335 aa. Contains multiple possible membrane spanning hydrophobic domains. 2 2 7 10 [Reporter]SCO2926 (12J19)_PCR/2661 [Gene]SCO2926/2423 NC_003888 SCE19A.26, putative secreted protein, len: 451 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Alternaive start codons are present at codons 11 and 17. Contains possible N-terminal region signal peptide sequence 2 1 13 4 [Reporter]SCO6286 (18J20)_PCR/771 [Gene]SCO6286/704 NC_003888 SC1G7.12c, possible regulatory protein, len: 24 aa; similar to TR:O24739 (EMBL:AB001609) Streptomyces virginiae butyrolactone autoregulator receptor protein BarB, 216 aa; fasta scores: opt: 565 Z-score: 645.4 bits: 126.6 E(): 2.3e-28; 48.980% identity in 196 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins 2 2 7 9 [Reporter]SCO1531 (13F19)_PCR/2660 [Gene]SCO1531/2422 NC_003888 SCL2.21c, thrS, threonine-tRNA synthetase, len: 658 aa; highly similar to SW:SYT_MYCTU (EMBL:Z95387) Mycobacterium tuberculosis threonyl-tRNA synthetase (EC 6.1.1.3) thrrS, 692 aa; fasta scores: opt: 1982 z-score: 2236.3 E(): 0; 65.1% identity in 659 aa overlap and to SW:SYT_ECOLI (EMBL:V00291) Escherichia coli threonyl-tRNA synthetase (EC 6.1.1.3) (threonine--tRNA ligase) TrhS, 642 aa; fasta scores: opt: 1335 z-score: 1506.4 E(): 0; 38.1% identity in 654 aa overlap. Contains Pfam match to entry PF00587 tRNA-synt_2b, tRNA synthetases class II (G, H, P and S) and two Prosite matches to entries PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1 and PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2 2 1 13 3 [Reporter]SCO5352 (19F20)_PCR/770 [Gene]SCO5352/703 NC_003888 SCBAC5H2.21, possible arginyl tRNA synthetase, len: 347 aa; similar to SW:SYR_BRELA (EMBL:Z21501) Brevibacterium lactofermentum arginyl tRNA synthetase (EC 6.1.1.19) ArgS, 550 aa; fasta scores (to C-terminal region): opt: 234 z-score: 260.2 E(): 6.4e-07; 36.0% identity in 161 aa overlap and blastp scores (to N-terminal region): Score = 182 (64.1 bits), Expect = 1.2e-28, Sum P(2) = 1.2e-28; Identities = 45/116 (38%), Positives = 65/116 (56%) and similar to SW:SYR_MYCTU (EMBL:Z73419) Mycobacterium tuberculosis arginyl t-RNA synthetase (EC 6.1.1.19) ArgS or MTCY373.12, 550 aa; fasta scores: opt: 216 z-score: 240.5 E(): 8.1e-06; 28.1% identity in 363 aa overlap 2 2 7 8 [Reporter]SCO1901 (14B19)_PCR/2659 [Gene]SCO1901/2421 NC_003888 SCI7.19, probable zinc-binding dehydrogenase, len: 329 aa; similar to many e.g. SW:DHSO_BACSU sorbitol dehydrogenase from Bacillus subtilis (352 aa) fasta scores; opt: 552, z-score: 626.6, E(): 1.4e-27, (30.1% identity in 345 aa overlap). Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and Prosite match to PS00059 Zinc-containing alcohol dehydrogenases signature 2 1 13 2 [Reporter]SCO7706 (20N16)_PCR/769 [Gene]SCO7706/702 NC_003888 SCBAC12C8.07c, putative DNA-binding protein, len: 284aa: similar to many from Streptomyces coelicolor eg. TR:Q9ADL0 (EMBL:AL451182) putative DNA-binding protein 2SCK31.04c (291 aa) fasta scores; opt: 731, Z-score: 816.6, 47.778% identity in 270 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix. 2 2 7 7 [Reporter]SCO0276 (15N15)_PCR/2658 [Gene]SCO0276/2420 NC_003888 SCF85.04, possible oxidoreductase, len: 417 aa. Weakly similar to many including: Bacillus subtilis TR:O34371 (EMBL:AF008220) YteT (428 aa), fasta scores opt: 296 z-score: 340.2 E(): 1.3e-11 25.3% identity in 379 aa overlap and Alcaligenes sp. (strain BR60) SW:CBAC_ALCSB (EMBL; U18133) 1-carboxy-3-chloro-3,4-dihydroxycyclo hexa-1,5-diene dehydrogenase (497 aa), fasta scores opt: 300 z-score: 345.3 E(): 7e-12 32.4% identity in 216 aa overlap. Contains a Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family 2 1 12 22 [Reporter]SCO1274 (1N12)_PCR/767 [Gene]SCO1274/701 NC_003888 2SCG18.21, unknown, len: 219 aa 2 1 12 21 [Reporter]SCO7110 (2J12)_PCR/766 [Gene]SCO7110/700 NC_003888 SC4B10.11, fdxA, ferredoxin, len: 129 aa; highly similar to SW:FER_STRGR (EMBL:S08287) Streptomyces griseus ferredoxin 7Fe, 105 aa; fasta scores: opt: 510 z-score: 644.4 E(): 2e-28; 64.4% identity in 104 aa overlap. Contains Pfam match to entry PF00037 fer4, 4Fe-4S binding domain and match to Prosite entry PS00190 Cytochrome c family heme-binding site signature and PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature 2 2 7 6 [Reporter]SCO0689 (16J15)_PCR/2657 [Gene]SCO0689/2419 NC_003888 SCF15.10, possible oxidoreductase, iron-sulphur binding subunit, len: 187 aa. Similar to many multipartite proteins including: the small subunit of quinoline 2-oxidoreductase from Pseudomonas putida TR:P72223(EMBL:X98131) QorS (168 aa), fasta scores opt: 371 z-score: 438.8 E(): 4.6e-17 44.7% identity in 170 aa overlap and Acetobacter europaeus TR:O30328(EMBL:Y08696) aldehyde dehydrogenase subunit III precursor (157 aa), fasta scores opt: 309 z-score: 367.9 E(): 4e-13 39.3% identity in 163 aa overlap. Also similar to the N-terminal iron-sulphur binding domain of Rhodobacter capsulatus (Rhodopseudomonas capsulata) TR:O54050(EMBL:AJ001013) xanthine dehydrogenase (EC 1.1.1.204) (462 aa), fasta scores opt: 299 z-score: 349.6 E(): 4.2e-12 37.2% identity in 164 aa overlap. Contains Pfam matches to entries PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains and PF01799 fer2_2, 2Fe-2S iron-sulfur cluster binding domains. Also contains Prosite hits to PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature and PS00070 Aldehyde dehydrogenases cysteine active site. 2 2 7 5 [Reporter]SCO0896 (17F15)_PCR/2656 [Gene]SCO0896/2418 NC_003888 SCM1.29c, possible oxidoreductase, len: 141 aa. Similar to many including Methanopyrus kandleri SW:IMDH_METKA (EMBL:U31567) putative inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) (172 aa), fasta scores opt: 227 z-score: 288.9 E(): 9.5e-09 31.6% identity in 117 aa overlap and Bacillus subtilis SW:YHCV_BACSU (EMBL:X96983) hypothetical 14.9 KD protein (140 aa), fasta scores opt: 295 z-score: 372.8 E(): 2e-13 40.0% identity in 135 aa overlap. Contains 2x Pfam match to entry PF00571 CBS, CBS domains. 2 2 7 4 [Reporter]SCO2940 (18B15)_PCR/2655 [Gene]SCO2940/2417 NC_003888 SCE56.04c, possible oxidoreductase, len: 773 aa; similar to TR:Q9ZBN7 (EMBL:AL034492) Streptomyces coelicolor putative oxidoreductase SC6C5.08, 828 aa; fasta scores: opt: 1088 z-score: 1144.8 E(): 0; 42.2% identity in 782 aa overlap and to SW:XDH_DROPS (EMBL:M33977) Drosophila pseudoobscura xanthine dehydrogenase (EC 1.1.1.204) Xdh, 1342 aa; fasta scores: opt: 517 z-score: 540.4 E(): 1.1e-22; 27.4% identity in 762 aa overlap. Contains Pfam match to entry PF01315 Ald_Xan_dh_C, Aldehyde oxidase and xanthine dehydrogenase, C terminus 2 2 7 3 [Reporter]SCP1.102 (19N11)_PCR/2654 [Gene]SCP1.102/2416 NC_003888 SCP1.102, possible plasmid transfer protein, (ATP/GTP-binding), len: 702aa; regions of similarity to proteins from other streptomycete plasmids. N-terminal similar to TR:Q07193 (EMBL:Z19593) SpdA protein from plasmid pSAM2 of Streptomyces ambofaciens (224 aa) blastp scores: Identities = 32/84 (38%), Positives = 38/84 (45%). C-terminal region similar to TR:Q06256 (EMBL:X72857) TraA protein from plasmid SCP2 of Streptomyces coelicolor (442 aa) blastp scores: Identities = 73/285 (25%), Positives = 130/285 (45%). Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 2 2 7 2 [Reporter]SCO1863 (20F11)_PCR/2653 [Gene]SCO1863/2415 NC_003888 SCI39.10c, unknown, len: 406 aa. High content in arginine amino acid residues and also highly hydrophilic 2 2 6 22 [Reporter]SCO5242 (1F7)_PCR/2651 [Gene]SCO5242/2414 NC_003888 2SC7G11.04, possible integral membrane protein, len: 152 aa; similar to TR:O05849 (EMBL:Z95120) Mycobacterium tuberculosis hypothetical 14.3 kDa protein MTCY07D11.09, 143 aa; fasta scores: opt: 177 z-score: 215.5 E(): 0.00015; 33.8% identity in 136 aa overlap 2 2 6 21 [Reporter]SCO1428 (2B7)_PCR/2650 [Gene]SCO1428/2413 NC_003888 SC6D7.11, acd, acyl-CoA dehydrogenase (EC 1.3.99.-), len: 391 aa. Highly similar to many Prokaryotic and eukaryotic acyl-CoA dehydrogenases including: Megasphaera elsdenii SW:ACDS_MEGEL(EMBL:L04528) acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2) (383 aa), fasta scores opt: 926 z-score: 1026.2 E():0 40.9% identity in 372 aa overlap and Homo sapiens (Human) SW:ACDS_HUMAN(EMBL:M26393) acyl-CoA dehydrogenase, short-chain precursor (EC 1.3.99.2) (412 aa, fasta scores opt: 924 z-score: 1023.5 E():0 41.2% identity in 376 aa overlap. Also similar to Streptomyces coelicolor TR:CAB46799(EMBL:AL096811) putative acyl-CoA dehydrogenase SCI30A.22C (383 aa), fasta scores opt: 940 z-score: 1041.6 E(): 0 41.1% identity in 375 aa overlap. Contains a Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase and a Prosite hit to PS00073 Acyl-CoA dehydrogenases signature 2. 2 2 6 20 [Reporter]SCO4653 (3N3)_PCR/2649 [Gene]SCO4653/2412 NC_003888 SCD82.25, rplL, 50S ribosomal protein L7/L12, len: 126 aa; identical to previously sequenced SW:RL7_STRCO (EMBL:L24552) Streptomyces coelicolor 50S ribosomal protein L7/L12 rplL. Contains Pfam match to entry PF00542 Ribosomal_L12, Ribosomal protein L7/L12 C-terminal domain 2 2 6 19 [Reporter]SCO6498 (4J3)_PCR/2648 [Gene]SCO6498/2411 NC_003888 SC1E6.07, unknown, len: 319 aa; similar to hypothetical protein from Mycobacteria e.g. TR:O69697 (EMBL:AL022121) MTV025.078C (346 aa), fasta scores; opt: 537 z-score: 578.0 E(): 6.5e-25, 34.9% identity in 292 aa overlap 2 2 6 18 [Reporter]SCO6350 (5F3)_PCR/2647 [Gene]SCO6350/2410 NC_003888 SC3A7.18c, unknown, len: 194 aa 2 2 6 17 [Reporter]SCO6633 (6B3)_PCR/2646 [Gene]SCO6633/2409 NC_003888 SC4G2.07, possible transcriptional regulator, len: 636 aa; similar to part of AFSR_STRCO regulatory protein AfsR (993 aa), fasta scores; opt: 512 z-score: 733.8 E(): 0, 35.5% identity in 521 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop) 2 2 6 15 [Reporter]SCO6970 (7J23)_PCR/2644 [Gene]SCO6970/2408 NC_003888 SC6F7.23c. possible membrane transport protein, len: 714 aa. Similar to many including: Bacillus subtilis TR:P96712(EMBL:D50098) multi drug transporter Bmr3 (512 aa), fasta scores opt: 1027 z-score: 1100.4 E():0 34.5% identity in 498 aa overlap. Also similar to several from Streptomyces coelicolor e.g. TR:Q9ZBJ1(EMBL:AL035161) putative efflux protein, SC9C7.19 (815 aa), fasta scores opt: 1639 z-score: 1751.6 E():0 49.4% identity in 530 aa overlap. Contains a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and multiple possible membrane spanning hydrophobic domains. 2 2 6 14 [Reporter]SCO5944 (8F23)_PCR/2643 [Gene]SCO5944/2407 NC_003888 SC7H1.14, unknown, len: 382 aa 2 2 6 13 [Reporter]SCO6064 (9B23)_PCR/2642 [Gene]SCO6064/2406 NC_003888 SC9B1.11, probable ABC transport permease, len: 221aa; similar to many eg. TR:O69722 (EMBL:AL022121) transport system permease from Mycobacterium tuberculosis (239 aa) fasta scores; opt: 643, z-score: 736.8, E(): 0, (47.7% identity in 220 aa overlap) and SW:PROW_BACSU glycine betaine/L-proline transport permease from Bacillus subtilis (217 aa) fasta scores; opt: 358, z-score: 415.9, E(): 7.6e-16, (28.2% identity in 195 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. 2 2 6 12 [Reporter]SCO2735 (10N19)_PCR/2641 [Gene]SCO2735/2405 NC_003888 SCC57A.06, possible integral membrane protein, len: 160 aa. Similar to Streptomyces coelicolor TR:O69999(EMBL:AL022374) putative integral membrane protein SC5B8.13 (174 aa), fasta scores opt: 186 z-score: 231.3 E(): 1.7e-05 34.5% identity in 148 aa overlap. Contains possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence. 2 2 6 11 [Reporter]SCO4618 (11J19)_PCR/2640 [Gene]SCO4618/2404 NC_003888 SCD39.18c, unknown, len: 283 aa 2 2 6 10 [Reporter]SCO4299 (12F19)_PCR/2639 [Gene]SCO4299/2403 NC_003888 SCD95A.32, possible integral membrane protein, len: 115 aa. Contains possible hydrophobic membrane spanning regions. High content in glycine amino acid residues 2 2 6 9 [Reporter]SCO0916 (13B19)_PCR/2638 [Gene]SCO0916/2402 NC_003888 SCM10.04c, unknown, len: 163 aa 2 2 6 8 [Reporter]SCO3175 (14N15)_PCR/2637 [Gene]SCO3175/2401 NC_003888 SCE87.26, possible acetyltransferase, len: 161 aa. Similar to Azospirillum brasilense SW:IAAT_AZOBR (EMBL:X57853) IAA acetyltransferase (EC 2.3.1.-) (153 aa), fasta scores opt: 224 z-score: 280.9 E(): 2.8e-08 36.7% identity in 139 aa overlap. Contains a Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 2 2 6 7 [Reporter]SCO3569 (15J15)_PCR/2636 [Gene]SCO3569/2400 NC_003888 SCH17.03c, probable endonuclease, len: 250 aa; similar to many e.g. SW:UVEN_MICLU (EMBL:U22181), pdg, Micrococcus luteus ultraviolet N-glycosylase/AP lyase (279 aa), fasta scores; opt: 1012 z-score: 970.1 E(): 0, 61.3% identity in 261 aa overlap. Weakly similar to SCE94.06, mutY (EMBL:AL049628) S.coelicolor putative adenine glycosylase (308 aa) (30.3% identity in 201 aa overlap), differing at C-terminus. Contains Pfam match to entry PF00730 Endonuclease_3, Endonuclease III, PS01155 Endonuclease III family signature1 and PS00764 Endonuclease III iron-sulfur binding region signature 4 4 22 4 [Reporter]SCO5340 (19P1)_PCR/8024 [Gene]SCO5340/7299 NC_003888 SCBAC5H2.09, unknown, len: 65 aa 4 4 22 3 [Reporter]SCP1.159c (20L1)_PCR/8023 [Gene]SCP1.159c/7298 NC_003888 SCP1.159c, possible oxidoreductase subunit, len: 90aa; similar to the extreme C-terminus of many glycerol-3-phosphate dehydrogenases eg. SW:P37606 (GPDA_ECOLI) glycerol-3-phosphate dehydrogenase from Escherichia coli (339 aa) fasta scores; opt: 176, z-score: 239.8 E(): 6.9e-06, 39.5% identity in 76 aa overlap and TR:Q9ZBS0 (EMBL:AL034447) glycerol-3-phosphate dehydrogenase from Streptomyces coelicolor (336 aa) fasta scores; opt: 252, z-score: 338.2, E(): 2.3e-11, 53.1% identity in 81 aa overlap. Also similar to neighbouring CDS SCP1.162c fasta scores; opt: 238, z-score: 294.0, E(): 7.6e-12, 50.7% identity in 75 aa overlap. 1 2 8 22 [Reporter]SCO4225 (1M7)_PCR/2191 [Gene]SCO4225/1999 NC_003888 2SCD46.39c, possible integral membrane protein, len: 130aa; contains possible membrane-spanning hydrophobic regions. 4 4 21 22 [Reporter]SCO1138 (1D21)_PCR/8020 [Gene]SCO1138/7297 NC_003888 2SCG38.31, possible secreted protein, len: 332 aa; similar to TR:AAG04143 (EMBL:AE004510) Pseudomonas aeruginosa hypothetical 34.9 kD protein PA0754, 327 aa; fasta scores: opt: 503 z-score: 536.4 E(): 2e-22; 29.0% identity in 328 aa overlap. Contains possible N-terminal region signal peptide sequence 1 2 8 21 [Reporter]SCO1849 (2I7)_PCR/2190 [Gene]SCO1849/1998 NC_003888 SCI8.34, cobN, CobN protein, len: 1217 aa. Highly similar to many e.g. Mycobacterium tuberculosis TR: O53498 (EMBL: AL021922) CobN (1194 aa), fasta scores opt: 2277 z-score: 2513.8 E(): 0 62.2% identity in 1224 aa overlap and Pseudomonas denitrificans SW:COBN_PSEDE (EMBL: M62866) CobN protein (1275 aa), fasta scores opt: 2437 z-score: 2690.5 E(): 0 41.6% identity in 1258 aa overlap. Also similar to metal chelatases e.g. Heliobacillus mobilis TR:Q9ZGE5 (EMBL: AF080002) Mg chelatase subunit H (1292 aa), fasta scores opt: 1647 z-score: 1815.7 E(): 0 31.4% identity in 1145 aa overlap. Contains a possible membrane spanning hydrophobic domain. 4 4 21 21 [Reporter]SCO2700 (2P17)_PCR/8019 [Gene]SCO2700/7296 NC_003888 SCC61A.21c, melC2, tyrosinase (monophenol monooxygenase), len: 288 aa; strongly similar to many eg. SW:P06845 (TYRO_STRGA) tyrosinase from Streptomyces glaucescens (273 aa) fasta scores; opt: 682, z-score: 837.0, E(): 0, 52.0% identity in 277 aa overlap. Contains Pfam match to entry PF00264 tyrosinase, Common central domain of tyrosinase Prosite match to PS00497 Tyrosinase CuA-binding region signature and Prosite match to PS00498 Tyrosinase and hemocyanins CuB-binding region signature. 1 2 8 20 [Reporter]SCO4145 (3E7)_PCR/2189 [Gene]SCO4145/1997 NC_003888 SCD84.12c, ppk, polyphosphate kinase, len: 774 aa; similar to TR:AAD29112 (EMBL:AF087931) Pseudomonas aeruginosa olyphosphate kinase Ppk, 690 aa; fasta scores: opt: 1802 z-score: 1999.8 E(): 0; 44.2% identity in 684 aa overlap and to SW:PPK_ECOLI (EMBL:L03719) Escherichia coli polyphosphate kinase (EC 2.7.4.1) Pkk, 687 aa; fasta scores: opt: 1262 z-score: 1400.2 E(): 0; 34.7% identity in 657 aa overlap 4 4 21 19 [Reporter]SCO5602 (4H17)_PCR/8017 [Gene]SCO5602/7295 NC_003888 SC2E1.19, unknown, len: 130 aa; similar to M. tuberculosis hypothetical protein YX29_MYCTU MTCY274.29c (128 aa), fasta scores; opt: 253 z-score: 276.8 E(): 3.5e-08, 39.7% identity in 121 aa overlap 1 2 8 19 [Reporter]SCO6047 (4A7)_PCR/2188 [Gene]SCO6047/1996 NC_003888 SC1B5.07, probable ABC-transporter ATP-binding protein, len: 284 aa; similar to many e.g. NOSF_PSEST P19844 pseudomonas stutzeri copper transport atp-binding protein nosF (308 aa), fasta scores; opt: 327 z-score: 381.3 E(): 5.2e-14, 30.5% identity in 292 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and Pfam match to entry ABC_tran PF00005, ABC transporters, score 126.83 4 4 21 18 [Reporter]SCO2406 (5D17)_PCR/8016 [Gene]SCO2406/7294 NC_003888 SC4A7.34, probable sugar transport permease, len: 414 aa; similar to TR:AAD45714 (EMBL:AF160811) Bacillus stearothermophilus L-arabinose membrane permease AraH, 407 aa; fasta scores: opt: 1448 z-score: 1515.1 E(): 0; 56.9% identity in 385 aa overlap and to SW:XYLH_HAEIN (EMBL:U32791) Haemophilus influenzae xylose transport permease protein XylH, 375 aa; fasta scores: opt: 970 z-score: 1017.9 E(): 0; 42.6% identity in 376 aa overlap 1 2 8 18 [Reporter]SCO5889 (5M3)_PCR/2187 [Gene]SCO5889/1995 NC_003888 SC3F7.09, redO, unknown, len: 87 aa 4 4 21 17 [Reporter]SCO6745 (6P13)_PCR/8015 [Gene]SCO6745/7293 NC_003888 SC5F2A.28c, alanine-rich hypothetical protein, len: 292 aa; similar to TR:Q9AK27 (EMBL:AL589164) Streptomyces coelicolor hypothetical protein 2SCK8.25, 292 aa; fasta scores: opt: 716 Z-score: 806.0 E(): 2.9e-37; 44.891% identity in 274 aa overlap 1 2 8 17 [Reporter]SCO6660 (6I3)_PCR/2186 [Gene]SCO6660/1994 NC_003888 SC5A7.10c, unknown, len: 311 aa; similar to a hypothetical protein from M. tuberculosis TR:O06813 (EMBL:Z95844) MTCY493.08 (303 aa), fasta scores; opt: 847 z-score: 1016.2 E(): 0, 47.7% identity in 304 aa overlap. Also some similarity to OPCA_SYNP7 putative oxpp cycle protein OpCA (which may be involved in functional assembly of glucose 6-phosphate dehydrogenase) (444 aa), fasta scores; opt: 149 z-score: 218.9 E(): 5.9e-05, 32.4% identity in 142 aa overlap. Note that both of these proteins are downstream of glucose-6-phosphate dehydrogenase genes 4 4 21 16 [Reporter]SCO1423 (7L13)_PCR/8014 [Gene]SCO1423/7292 NC_003888 SC6D7.16, possible glycosyl transferase, len: 302 aa. Similar to many eukaryotic dolichol-phosphate-mannose synthases e.g. Mus musculus (Mouse) TR:O70152 (EMBL:AB004789) (260 aa), fasta scores opt: 474 z-score: 560.2 E(): 7.9e-24 37.9% identity in 227 aa overlap. Also similar to a number of Prokaryotic proteins of undefined function e.g. Mycobacterium leprae TR:O32929 (EMBL:Z98604) hypothetical 30.1 KD protein (277 aa), fasta scores opt: 917 z-score: 1076.0 E():0 52.0% identity in 281 aa overlap. Contains a Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases. 1 2 8 16 [Reporter]SCO0314 (7E3)_PCR/2185 [Gene]SCO0314/1993 NC_003888 SC5G9.23, putative secreted protein, len: 648 aa; unknown function, similar to TR:Q52556 (EMBL:M86744), SdsA, Pseudomonas sp. alkyl sulfatase which degrades sulfate esters of long-chain primary alcohols (SDS) (528 aa), fasta scores; opt: 739 z-score: 806.7 E(): 0, 29.7% identity in 528 aa overlap, but having an N-terminal extension of approx. 140 aa. Similar to TR:Q48790 (EMBL:X92423), SepA, Listeria monocytogenes gene implicated in cell separation (391 aa) (42.6% identity in 364 aa overlap). Also similar to hypothetical proteins e.g. TR:O69728 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical protein (626 aa) (52.9% identity in 624 aa overlap). Contains Pfam match to entry PF00753 lactamase_B, Metallo-beta-lactamase superfamily. Contains possible N-terminal region signal sequence peptide 4 4 21 15 [Reporter]SCO5213 (8H13)_PCR/8013 [Gene]SCO5213/7291 NC_003888 SC7E4.10c, possible integral membrane protein, len: 240 aa; similar to TR:CAB89063 (EMBL:AL353872) Streptomyces coelicolor putative integral membrane protein SC5G8.12, 322 aa; fasta scores: opt: 126 z-score: 158.5 E(): 0.23; 25.6% identity in 160 aa overlap. Contains possible hydrophobic membrane spanning regions 1 2 8 15 [Reporter]SCO2156 (8A3)_PCR/2184 [Gene]SCO2156/1992 NC_003888 SC6G10.29c, cox2, cytochrome c oxidase subunit II, len: 319aa; similar to many eg. SW:COX2_MYCTU cox2, PUTATIVE cytochrome c oxidase subunit II from Mycobacterium tuberculosis (363 aa) fasta scores; opt: 417, z-score: 477.1, E(): 2.8e-19, (35.4% identity in 308 aa overlap). Contains two Pfam match to entry PF00116 COX2, Cytochrome C oxidase subunit II and Prosite match to PS00078 CO II and nitrous oxide reductase dinuclear copper centers signature. 4 4 21 14 [Reporter]SCO5414 (9D13)_PCR/8012 [Gene]SCO5414/7290 NC_003888 SC8F4.18, putative membrane protein, len: 109 aa; previously sequenced therefore identical to TR:Q9X948 (EMBL:AJ237976). Also similar to SW:Z75555 (Y076_MYCTU) hypothetical protein from Mycobacterium tuberculosis (120 aa) fasta scores; opt: 248, z-score: 330.5, E(): 5.3e-11, 37.5% identity in 96 aa overlap. Contains possible hydrophobic membrane spanning regions 1 2 8 14 [Reporter]SCO2419 (8M23)_PCR/2183 [Gene]SCO2419/1991 NC_003888 SC8A2.07c, possible secreted protein, len: 418 aa; similar to TR:O53967 (EMBL:AL022073) Mycobacterium tuberculosis mce-family protein MTV051.04, 425 aa; fasta scores: opt: 548 z-score: 564.7 E(): 6.2e-24; 33.0% identity in 367 aa overlap. Contains Pfam match to entry PF02470 mce. Also contains possible N-terminal region signal peptide sequence 1 2 8 13 [Reporter]SCO1503 (9I23)_PCR/2182 [Gene]SCO1503/1990 NC_003888 SC9C5.27c, possible secreted protein, len: 149 aa. Contains possible N-terminal region signal peptide sequence 4 4 21 13 [Reporter]SCO2565 (10P9)_PCR/8011 [Gene]SCO2565/7289 NC_003888 SCC123.03c, unknown, len: 73 aa. 4 4 21 12 [Reporter]SCO4322 (11L9)_PCR/8010 [Gene]SCO4322/7288 NC_003888 SCD12A.05, possible integral membrane protein, len: 366 aa. Contains possible hydrophobic membrane spanning regions at C-terminal domain. High content in alanine, arginine and glycine amino acid residues 1 2 8 12 [Reporter]SCO2724 (10E23)_PCR/2181 [Gene]SCO2724/1989 NC_003888 SCC46.09c, possible amino acid transporter, len: 476 aa; similar to TR:BAA87325 (EMBL:AB020520) Rattus norvegicus Y+L amino acid transporter 1, RT+Lat1, 512 aa; fasta scores: opt: 354 z-score: 394.6 E(): 1.5e-14; 26.3% identity in 460 aa overlap. Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease. Contains also possible hydrophobic membrane spanning regions 4 4 21 11 [Reporter]SCO4480 (12H9)_PCR/8009 [Gene]SCO4480/7287 NC_003888 SCD65.23c, possible tetR-family transcriptional regulatory protein, len: 205 aa; similar to TR:O53661 (EMBL:AL021929) Mycobacterium tuberculosis putative regulatory protein MTV034.04, 204 aa; fasta scores: opt: 193 z-score: 241.7 E(): 5.4e-06; 25.7% identity in 187 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 37..58 (+2.77 SD) 1 2 8 11 [Reporter]SCO4094 (11A23)_PCR/2180 [Gene]SCO4094/1988 NC_003888 SCD25.30, unknown, len: 116 aa. Highly similar to Mycobacterium tuberculosis TR:P96242 (EMBL; Z83864) hypothetical 15.0 KD protein (137 aa), fasta scores opt: 395 z-score: 500.7 E(): 1.5e-20 54.4% identity in 114 aa overlap. Contains a Prosite hit to PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. 4 4 21 10 [Reporter]SCO3241 (13D9)_PCR/8008 [Gene]SCO3241/7286 NC_003888 SCE29.10c, possible isomerase, len: 290 aa; similar to TR:O69950 (EMBL:AL023862) hypothetical protein from Streptomyces coelicolor (315 aa) fasta scores; opt: 1116, z-score: 1331.4, E(): 0, (62.5% identity in 283 aa overlap). Also similar to SW:XYLA_STRDI xylose isomerase from Streptomyces diastaticus (388 aa) fasta scores; opt: 122, z-score: 148.8, E(): 0.56,25.0% identity in 296 aa overlap. 1 2 8 10 [Reporter]SCO2930 (12M19)_PCR/2179 [Gene]SCO2930/1987 NC_003888 SCE19A.30, probable permease membrane component, len: 215 aa; similar to components transporters involved in osmoregulation e.g. SW:PROW_BACSU (EMBL:U38418), proW, Bacillus subtilis glycine betaine/L-proline transport system permease protein (217 aa), fasta scores; opt: 413 z-score: 474.7 E(): 4.2e-19, 35.1% identity in 211 aa overlap. Similar to SCE19A.32, probable permease inner membrane component (290 aa) (33.8% identity in 207 aa overlap) and to SC9B1.11 (EMBL:AL049727) S.coelicolor probable ABC transport permease (221 aa) (31.3% identity in 195 aa overlap). Contains hydrophobic, possible membrane-spanning regions. May be part of a multicomponent transporter which includes SCE19A.30, SCE19A.31, SCE19A.32 and SCE19A.33. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component 4 4 21 9 [Reporter]SCO5165 (14P5)_PCR/8007 [Gene]SCO5165/7285 NC_003888 SCP8.28c, possible hydrolase, len: 298 aa; similar to TR:Q9RL59 (EMBL:AL121596) Streptomyces coelicolor putative hydrolase SCF51A.05, 278 aa; fasta scores: opt: 217 z-score: 249.2 E(): 2e-06; 27.0% identity in 267 aa overlap and to SW:THCF_RHOER (EMBL:U95170) Rhodococcus erythropolis non-heme haloperoxidase (EC 1.11.1.-) ThcF, 274 aa; fasta scores: opt: 185 z-score: 213.7 E(): 0.00019l; 29.0% identity in 241 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold and match to Prosite entry PS00120 Lipases, serine active site 1 2 8 9 [Reporter]SCO3678 (13I19)_PCR/2178 [Gene]SCO3678/1986 NC_003888 SCH35.46, deoxynucleotide triphosphate deaminase, len: 191 aa; highly similar to many e.g. TR:O07247 (EMBL:Z96800) Dcd from Mycobacterium tuberculosis (190 aa) fasta scores; opt: 981, z-score: 1187.0, E(): 0, (74.6% identity in 189 aa overlap) and SW:DCD_ECOLI dCTP deaminase from Escherichia coli (193 aa) fasta scores; opt: 405, z-score: 496.7, E(): 2.5e-20, (43.9% identity in 189 aa overlap). Contains Pfam match to entry PF00692 dUTPase, dUTPase and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 4 21 8 [Reporter]SCO1174 (15L5)_PCR/8006 [Gene]SCO1174/7284 NC_003888 SCG11A.05, thcA, aldehyde dehydrogenase, len:534 aa; highly similar to SW:THCA_RHOSN (EMBL:U17129) Rhodococcus sp. (strain NI86/21) EPTC-inducible aldehyde dehydrogenase (EC 1.2.1.3) ThcA, 505 aa; fasta scores: opt: 2639 z-score: 3078.6 E(): 0; 75.7% identity in 506 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase and two matches to Prostie entries PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site 1 2 8 8 [Reporter]SCO1610 (14E19)_PCR/2177 [Gene]SCO1610/1985 NC_003888 SCI35.32c, possible membrane protein, len: 319 aa; similar to TR:Q9XAN5 (EMBL:AL079355) Streptomyces coelicolor putative membrane protein SC4C6.05c, 347 aa; fasta scores: opt: 474 Z-score: 431.9 E(): 2e-16; 37.853% identity in 354 aa overlap. Contains possible transmembrane domain at around aa 80 4 4 21 7 [Reporter]SCO3801 (16H5)_PCR/8005 [Gene]SCO3801/7283 NC_003888 SCGD3.02, probable aminopeptidase, len: 432 aa; similar to many eukaryotic aminopeptidases e.g. (EMBL:AF005050) Homo sapiens aspartyl aminopeptidase (472 aa), fasta scores; opt: 474 z-score: 541.2 E(): 8.1e-23, 34.7% identity in 427 aa overlap. Also similar to TR:Q50022 (EMBL:Z95151), pepX, Mycobacterium leprae aminopeptidase (443 aa) (48.7% identity in 425 aa overlap) 1 2 8 7 [Reporter]SCO5152 (15A19)_PCR/2176 [Gene]SCO5152/1984 NC_003888 SCP8.15c, probable ATP-binding protein, len: 371 aa; similar to SW:MRP_MYCTU (EMBL:Z98260) Mycobacterium tuberculosis Mrp protein homolog MTV006.01c, 390 aa; fasta scores: opt: 1650 z-score: 1730.0 E(): 0; 67.9% identity in 371 aa overlap and to SW:MRP_ECOLI (EMBL:U00007) Escherichia coli Mrp protein, 379 aa; fasta scores: opt: 706 z-score: 744.5 E(): 0; 35.8% identity in 363 aa overlap. Contains Pfam matches to entries PF01883 DUF59, Domain of unknown function DUF59 and PF00142 fer4_NifH, 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS01215 Mrp family signature 4 4 21 6 [Reporter]SCO0668 (17D5)_PCR/8004 [Gene]SCO0668/7282 NC_003888 SCF91.28c, possible flavoprotein, len: 530 aa. Highly similar to two hypothetical proteins from Mycobacterium tuberculosis e.g. TR:O53525 (EMBL:AL021925) hypothetical 49.8 KD protein (457 aa), fasta scores opt: 1698 z-score: 1838.9 E():0 57.7% identity in 461 aa overlap. Also similar to several eukaryotic alkyldihydroxyacetonephosphate synthases (EC 2.5.1.26) e.g. from Homo sapiens (Human) SW:ADAS_HUMAN (EMBL:Y09443) (658 aa), fasta scores opt: 598 z-score: 648.1 E(): 1e-28 30.4% identity in 573 aa overlap. Contains a Pfam match to entry PF01565 FAD_binding_4, FAD binding domain. 1 2 8 6 [Reporter]SCO0360 (16M15)_PCR/2175 [Gene]SCO0360/1983 NC_003888 SCF41.19c, probable DNA-binding, LacI family, regulator. len: 330 aa. Similar to Escherichia coli SW:GNTR_ECOLI (EMBL:D84362) gluconate utilization system GNT-I transcriptional repressor (331 aa), fasta scores opt: 674 z-score: 767.8 E(): 0 36.9% identity in 320 aa overlap. Contains a PS00356 Bacterial regulatory proteins, lacI family signature, a PS00237 G-protein coupled receptors signature, Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family and a Pfam match to entry PF00356 lacI, Bacterial regulatory proteins, LacI family. Contains a possible helix-turn-helix motif between residues 2..23 score (+5.90 SD). 4 4 21 5 [Reporter]SCO3542 (18P1)_PCR/8003 [Gene]SCO3542/7281 NC_003888 SCH5.05c, probable integral membrane protein with kinase activity, len: 1089 aa; a multidomain protein. The N-terminal 480 aa (approx.) contains several probable membrane spanning hydrophobic regions. 510-710 aa similar to several thymidylate kinases e.g. SW:KTHY_ECOLI thymidylate kinase, Tmk, from Escherichia coli (213 aa) fasta scores; opt: 423, z-score: 339.7, E(): 1.3e-11, (38.4% identity in 198 aa overlap). 710-800 aa (approx.) glutamic acid-rich region which may form a hinge structure. 800-1089 aa has no distinguishing features or significant database similarities. Further clues to function may be indicated by the presence of matches to PS00217 Sugar transport proteins signature 2 and PS00017 ATP/GTP-binding site motif A (P-loop). Contains Probable coiled-coil from 711 to 875 (165 residues), max score: 2.135, (probability 1.00) 1 2 8 5 [Reporter]SCO3570 (17I15)_PCR/2174 [Gene]SCO3570/1982 NC_003888 SCH17.04, possible membrane protein, len: 111 aa; unknown function, probable highly hydrophobic CDS suggested by positional base preference, GC frame analysis and a putative RBS. Sequence is rich in Ala and Phe and contains 8x degenerate (A/V)V(S/F)FA repeats. Contains TTA (leucine) codon, possible target for bldA regulation 4 4 21 4 [Reporter]SCP1.11c (19L1)_PCR/8002 [Gene]SCP1.11c/7280 NC_003888 SCP1.11c, unknown, len: 247aa; 1 2 8 4 [Reporter]SCO3545 (18E15)_PCR/2173 [Gene]SCO3545/1981 NC_003888 SCH5.08c, possible transferase, len: 505 aa; limited similarity to many proteins of undefined function e.g. TR:O27384 (EMBL:AE000896) methyltransferase related protein (196 aa) fasta scores; opt: 162, z-score: 186.2, E(): 0.0046, (28.9% identity in 152 aa overlap). Contains PS00092 N-6 Adenine-specific DNA methylases signature 1 2 8 3 [Reporter]SCO4983 (19A15)_PCR/2172 [Gene]SCO4983/1980 NC_003888 2SCK36.06c, unknown, len: 140 aa 4 4 21 3 [Reporter]SCP1.88c (20H1)_PCR/8001 [Gene]SCP1.88c/7279 NC_003888 SCP1.88c, possible hydrolase, len: 193aa; weakly similar to many eg. TR:Q9X8G0 (EMBL:AL049819) putative hydrolase from Streptomyces coelicolor (193 aa) fasta scores; opt: 177, z-score: 224.6, E(): 4.8e-05, 30.7% identity in 153 aa overlap and SW:O07900 (VIBB_VIBCH) vibriobactin-specific isochorismatase (EC 3.3.2.1) (293 aa) fasta scores; opt: 163, z-score: 205.0, E(): 0.0006, 23.9% identity in 180 aa overlap. Contains Pfam match to entry PF00857 Isochorismatase, Isochorismatase family. 4 4 20 22 [Reporter]SCO1335 (1P17)_PCR/7998 [Gene]SCO1335/7278 NC_003888 2SCG61.17c, probable oxidoreductase, len: 246 aa; similar to many eg. SW:P40288 (DHG_BACME) glucose 1-dehydrogenase (EC 1.1.1.47) from Bacillus megaterium (261 aa) fasta scores; opt: 511, z-score: 535.5, E(): 2.4e-22, 36.6% identity in 243 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Pfam match to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus. 1 2 8 2 [Reporter]SCO3655 (20I11)_PCR/2171 [Gene]SCO3655/1979 NC_003888 SCH10.33c, possible spermidine synthase, len: 554 aa; C-terminal half similar to many e.g. SW:SPEE_ECOLI (EMBL:J02804), speE, Escherichia coli spermidine synthase (287 aa), fasta scores; opt: 300 z-score: 300.8 E(): 1.9e-09, 33.5% identity in 188 aa overlap. The whole CDS is similar to TR:O33279 (EMBL:AL00868), speE, Mycobacterium tuberculosis probable spermidine synthase (523 aa) (40.6% identity in 525 aa overlap) and TR:O67365 (EMBL:AE000736) Aquifex aeolicus hypothetical protein (493 aa) (31.6% identity in 434 aa overlap). Contains possible hydrophobic membrane spanning regions 4 4 20 20 [Reporter]SCO7644 (3H17)_PCR/7996 [Gene]SCO7644/7277 NC_003888 SC10F4.17c, possible ATP binding protein, len: 407 aa; similar to TR:Q9KRL3 (EMBL:AE004240) Vibrio cholerae hypothetical protein VC1624, 414 aa; fasta scores: opt: 582 z-score: 689.7 E(): 7e-31; 29.8% identity in 413 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 2 7 22 [Reporter]SCO4221 (1I7)_PCR/2169 [Gene]SCO4221/1978 NC_003888 2SCD46.35c, unknown, len: aa; 4 4 20 19 [Reporter]SCO5592 (4D17)_PCR/7995 [Gene]SCO5592/7276 NC_003888 SC2E1.09, unknown, len: 79 aa; highly similar to small hypothetical proteins from several organisms, e.g. YX40_MYCTU MTCY274.40c (80 aa), fasta scores; opt: 356 z-score:628.4 E(): 9.1e-28, 71.6% identity in 74 aa overlap 1 2 7 21 [Reporter]SCO6243 (2E7)_PCR/2168 [Gene]SCO6243/1977 NC_003888 SCAH10.08c, aceB1, malate synthase, len: 540 aa; highly similar to SW:MASY_STAE (EMBL:U63518) Streptomyces arenae, malate synthase (EC 4.1.3.2) AceB, 543 aa; fasta scores: opt: 2625 z-score: 3156.2 E(): 0; 78.2% identity in 551 aa overlap. Contains match to Pfam entry PF01274 Malate_synthase, Malate synthase and to Prosite entry PS00510 Malate synthase signature 4 4 20 18 [Reporter]SCO7671 (5P13)_PCR/7994 [Gene]SCO7671/7275 NC_003888 SC4C2.06c, possible transferase, len: 412 aa; similar to SW:BCSA_BACSU (EMBL:L77246) Bacillus subtilis putative chalcone synthase (EC 2.3.1.74) BcsA, 365 aa; fasta scores: opt: 353 z-score: 334.4 E(): 4.3e-11; 31.0% identity in 339 aa overlap. Contains 2x Pfam matches to entry PF00195 Chal_stil_synt, Chalcone and stilbene synthases and match to Prosite entry PS00196 Type-1 copper (blue) proteins signature. Also contains 9x degenerate repeat: GS 1 2 7 20 [Reporter]SCO7623 (3A7)_PCR/2167 [Gene]SCO7623/1976 NC_003888 SC2H2.21c, pntA, NAD(P) transhydrogenase alpha subunit, len: 524 aa; similar to SW:PNTA_ECOLI (EMBL:X04195) Escherichia coli NAD(P) transhydrogenase subunit alpha (EC 1.6.1.1) PntA, 510 aa; fasta scores: opt: 1907 z-score: 1729.3 E(): 0; 58.4% identity in 514 aa overlap. Contains Pfam match to entry PF01262 AlaDh_PNT, Alanine dehydrogenase/pyridine nucleotide transhydrogenase. Also contains possible hydrophobic membrane spanning regions 4 4 20 16 [Reporter]SCO5640 (7H13)_PCR/7992 [Gene]SCO5640/7274 NC_003888 SC6A9.27, unknown, len: 155 aa; this ORF has atypically low GC bias in FramePlot 1 2 7 19 [Reporter]SCO6510 (4M3)_PCR/2166 [Gene]SCO6510/1975 NC_003888 SC1E6.19c, unknown, len: 273 aa; similar to hypothetical proteins e.g. M. tuberculosis TR:O53851 (EMBL:AL022004) MTV043.32 (270 aa), fasta scores; opt: 1102 z-score: 1628.2 E(): 0, 58.6% identity in 263 aa overlap, and in part, to several methyltransferases e.g. PMTA_RHOSH phosphatidylethanolamine N-methyltransferase (203 aa), fasta scores; opt: 198 z-score: 248.3 E(): 1.5e-06, 37.3% identity in 102 aa overlap 4 4 20 15 [Reporter]SCO5208 (8D13)_PCR/7991 [Gene]SCO5208/7273 NC_003888 SC7E4.05c, probable monophosphatase, len: 266 aa; similar to SW:STRO_STRGR (EMBL:Y08763) Streptomyces griseus glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) StrO, 259 aa; fasta scores: opt: 316 z-score: 395.8 E(): 1.4e-14; 35.9% identity in 248 aa overlap. Contains 2x Pfam match to entry PF00459 inositol_P, Inositol monophosphatase family and match to Prosite entry PS00629 Inositol monophosphatase family signature 1 1 2 7 18 [Reporter]SCO5885 (5I3)_PCR/2165 [Gene]SCO5885/1974 NC_003888 SC3F7.05c, putative membrane protein, len: 146 aa. Contains possible hydrophobic membrane spanning regions 4 4 20 14 [Reporter]SCO6998 (9P9)_PCR/7990 [Gene]SCO6998/7272 NC_003888 SC8F11.24c, possible cytochrome P450, len: 407 aa. Similar to many possible cytochrome P450s including: Streptomyces griseolus SW:CPXF_STRGO (EMBL:M32239) cytochrome P450-SU2 (402 aa), fasta scores opt: 735 z-score: 827.8 E(): 0 34.3% identity in 408 aa overlap. Contains 2xPfam matches to entries PF00067 p450, Cytochrome P450. 4 3 3 5 [Reporter]SCO3656 (17H10)_PCR/5592 [Gene]SCO3656/5099 NC_003888 SCH10.34c, hypothetical protein, len: 185 aa; unknown function, similar to SW:Y0B3_MYCTU (EMBL:Z77724) Mycobacterium tuberculosis hypothetical protein (164 aa), fasta scores; opt: 197 z-score: 256.6 E(): 5.6e-07, 34.7% identity in 167 aa overlap 1 2 7 17 [Reporter]SCO6638 (6E3)_PCR/2164 [Gene]SCO6638/1973 NC_003888 SC4G2.12c, unknown, len: 605 aa; identical to partial S. coelicolor sequence TR:Q53945 (EMBL:L37531) ORF4 downstream of PglZ (>302 aa) and weakly similar to Y686_METJA hypothetical protein MJ0686 (Methanococcus jannaschii) (580 aa), fasta scores; opt: 260 z-score: 416.1 E(): 6.2e-16, 23.3% identity in 601 aa overlap. Contains TTA Leu codon; possible target for action of bldA 4 4 20 13 [Reporter]SCO2525 (10L9)_PCR/7989 [Gene]SCO2525/7271 NC_003888 SCC121.28c, unknown, len: 257 aa 4 3 3 4 [Reporter]SCO0588 (18D10)_PCR/5591 [Gene]SCO0588/5098 NC_003888 SCF55.12c, cvnA11, possible sensor kinase, len: 575 aa; low similarity to C-terminus Streptomyces coelicolor possible sensor kinase SC6G9.44c, 806 aa; fasta scores: opt: 380 z-score: 360.0 E(): 1.1e-12; 25.4% identity in 543 aa overlap and to N-terminus TR:CAB41562 (EMBL:AL049727) Streptomyces coelicolor putative large secreted protein SC9B1.16c, 877 aa; fasta scores: opt: 515 z-score: 485.5 E(): 1.1e-19; 31.9% identity in 433 aa overlap. Contains match to Pfam entry PF00512 signal, Histidine kinase and Prosite entry PS00152 ATP synthase alpha and beta subunits signature. Contains also possible hydrophobic membrane spanning regions 1 2 7 16 [Reporter]SCO0310 (7A3)_PCR/2163 [Gene]SCO0310/1972 NC_003888 SC5G9.19c, possible tetR-family transcriptional regulator, len: 141 aa; shows similarity to the N-terminal half of TR:O24741 (EMBL:AB001683), FarA, Streptomyces sp. strain FRI-5 receptor for an extracellular regulatory factor (IM-2) (221 aa), fasta scores; opt: 154 z-score: 193.4 E(): 0.0021, 36.7% identity in 79 aa overlap and to putative transcriptional regulators. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 4 4 20 12 [Reporter]SCO2561 (11H9)_PCR/7988 [Gene]SCO2561/7270 NC_003888 SCC77.28c, possible long-chain fatty-acid CoA ligase, len: 647 aa. Similar to many from eukaryotes and prokaryotes including: Homo sapiens (Human) SW:LCFB_HUMAN(EMBL:D10040) long-chain-fatty-acid-CoA ligase 2 (EC 6.2.1.3) (698 aa), fasta scores opt: 698 z-score: 755.0 E(): 0 29.6% identity in 612 aa overlap and Streptomyces coelicolor TR:Q9X7Z0(EMBL:AL049497) putative long chain fatty acid CoA ligase SC6G10.04 (598 aa), fasta scores opt: 1056 z-score: 1142.9 E():0 40.5% identity in 583 aa overlap. Contains a Pfam match to entry PF00501 AMP-binding and a Prosite hit to PS00455 Putative AMP-binding domain signature. 4 3 3 3 [Reporter]SCO4903 (19P6)_PCR/5590 [Gene]SCO4903/5097 NC_003888 2SCK8.29c, possible membrane protein, len: 67aa; similar to TR:O34719 (EMBL:AF017113) Bacillus subtilis YvlC protein, 65 aa; fasta scores: opt: 107 z-score: 169.7 E(): 0.069; 33.9% identity in 62 aa overlap. Contains possible hydrophobic membrane spanning region 1 2 7 15 [Reporter]SCO6973 (7M23)_PCR/2162 [Gene]SCO6973/1971 NC_003888 SC6F7.26c, unknown, len: 140 aa. Similar, in parts, to proteins containing the principally Eukaryotic ankyrin repeat motifs e.g. Chromatium vinosum SW:ANKH_CHRVI(EMBL:L13419) ankyrin homolog precursor (323 aa), fasta scores opt: 200 z-score: 247.0 E(): 2.5e-06 38.4% identity in 125 aa overlap. Contains 2xPfam matches to entry PF00023 ank, Ank repeat. 4 3 3 2 [Reporter]SCP1.208 (20H6)_PCR/5589 [Gene]SCP1.208/5096 NC_003888 SCP1.208, unknown, len: 346aa; 1 2 7 14 [Reporter]SCO2415 (8I23)_PCR/2161 [Gene]SCO2415/1970 NC_003888 SC8A2.03c, possible secreted protein, len: 413 aa; similar to TR:O53971 (EMBL:AL022073) Mycobacterium tuberculosis hypothetical 40.2 kDa protein MTV051.08, 377 aa; fasta scores: opt: 685 z-score: 681.0 E(): 2.1e-30; 36.0% identity in 311 aa overlap. Contains Pfam match to entry PF02470 mce. Also contains possible N-terminal region signal peptide sequence 4 3 2 22 [Reporter]SCO5192 (1H2)_PCR/5587 [Gene]SCO5192/5095 NC_003888 2SC3B6.16c, hypothetical protein, len: 469 aa; similar to TR:O53343 (EMBL:AL021646) Mycobacterium tuberculosis ABC-transporter ATP binding protein MTV014.41, 447 aa; fasta scores: opt: 1156 z-score: 1301.9 E(): 0; 45.3% identity in 393 aa overlap 4 3 2 21 [Reporter]SCO0731 (2D2)_PCR/5586 [Gene]SCO0731/5094 NC_003888 3SC5B7.09c, probable transferase, len: 444 aa; similar to SW:MALY_ECOLI (EMBL:M60722) Escherichia coli aminotransferase MalY protein, 390 aa; fasta scores: opt: 522 z-score: 589.7 E(): 2.5e-25; 30.8% identity in 370 aa overlap 4 3 2 20 [Reporter]SCO5226 (2P22)_PCR/5585 [Gene]SCO5226/5093 NC_003888 SC7E4.23c, nrdL, ribonucleotide-diphosphate reductase large chain, len: 791 aa; identical to previously sequenced TR:CAB82485 (EMBL:AJ276618) Streptomyces coelicolor A3(2) ribonucleotide-diphosphate reductase large chain (EC 1.17.4.1) NrdL, 791 aa. Contains Pfam match to entry PF00317 ribonucleo_red, Ribonucleotide reductase and match to Prosite entry PS00089 Ribonucleotide reductase large subunit signature 4 3 2 19 [Reporter]SCO1403 (3L22)_PCR/5584 [Gene]SCO1403/5092 NC_003888 SC1A8A.23, possible membrane protein, len: 180aa; contains possible membrane-spanning hydrophobic regions. 4 3 2 18 [Reporter]SCO6190 (4H22)_PCR/5583 [Gene]SCO6190/5091 NC_003888 SC2G5.11, possible transferase, len: 298aa; similar to many eg. TR:P74820 (EMBL:U51197) glycosyl transferase from Sphingomonas S88 (288 aa) fasta scores; opt: 167, z-score: 204.0, E(): 0.00045, (27.5% identity in 262 aa overlap). Contains Pfam match to entry PF00535 Glycos_transf_2,Glycosyl transferases, score 51.10, E-value 2.4e-11. NB this CDS is an imperfect duplicate of the neighbouring upstream CDS SC2G5.10 fasta scores; opt: 772, z-score: 804.0, E(): 0, (47.5% identity in 280 aa overlap). 4 3 2 17 [Reporter]SCO7127 (5D22)_PCR/5582 [Gene]SCO7127/5090 NC_003888 SC4B10.28c, hypothetical protein, len: 164 aa; similar to TR:Q9Z582 (EMBL:AL035569) Streptomyces coelicolor hypothetical protein SC8D9, 164 aa; fasta scores: opt:257 z-score: 326.3 E(): 1e-10; 34.1% identity in 132 aa overlap 4 4 20 11 [Reporter]SCO4476 (12D9)_PCR/7987 [Gene]SCO4476/7269 NC_003888 SCD65.19c, hypothetical protein, len: 473 aa; similar to TR:O07803 (EMBL:Z97188) Mycobacterium tuberculosis hypothetical 52.1 kDa protein MTCY409.10, 468 aa; fasta scores: opt: 575 z-score: 663.0 E(): 1.8e-29; 27.8% identity in 464 aa overlap 4 4 20 10 [Reporter]SCO3268 (13P5)_PCR/7986 [Gene]SCO3268/7268 NC_003888 SCE39.18c, putative secreted protein, len: 92 aa; similar to TR:CAC44706 (EMBL:AL596251) Streptomyces coelicolor conserved hypothetical protein SCBAC25E3.19, 92 aa; fasta scores: opt: 471 Z-score: 603.7 E(): 5.4e-26l; 81.522% identity in 92 aa overlap. Contains possible N-terminal region signal peptide sequence 1 2 7 13 [Reporter]SCO6067 (9E23)_PCR/2160 [Gene]SCO6067/1969 NC_003888 SC9B1.14c, cvnC6, unknown, len: 119 aa; similar to other hypothetical proteins egs. two from Streptomyces coelicolor: TR:O86520 (EMBL:AL031124) (190 aa) fasta scores; opt: 242, z-score: 321.0, E(): 1.5e-10, (39.8% identity in 118 aa overlap) and TR:O86523 (EMBL:AL031124) (132 aa) fasta scores; opt: 228, z-score: 305.7, E(): 1e-09, (39.3% identity in 112 aa overlap), and TR:O50392 (EMBL:AL009198) from Mycobacterium tuberculosis (122 aa) fasta scores; opt: 188, z-score: 255.2, E(): 6.8e-07, (37.5% identity in 120 aa overlap). 4 4 20 9 [Reporter]SCO0460 (14L5)_PCR/7985 [Gene]SCO0460/7267 NC_003888 SCF51A.38, unknown, len: 169 aa. 1 2 7 12 [Reporter]SCO2720 (10A23)_PCR/2159 [Gene]SCO2720/1968 NC_003888 SCC46.05c, possible long-chain fatty acid ligase, len: 642 aa; similar to TR:Q9ZBW6 (EMBL:AL034443) Streptomyces coelicolor putative long-chain-fatty-acid-CoA ligase SC4B5.02c, 608 aa; fasta scores: opt: 2039 z-score: 2214.0 E(): 0; 51.4% identity in 607 aa overlap and to SW:LCFB_HUMAN (EMBL:D10040) Homo sapiens long-chain-fatty-acid-CoA ligase 2 (EC 6.2.1.3) FacL2, 698 aa; fasta scores: opt: 471 z-score: 511.5 E(): 4.5e-21; 27.8% identity in 615 aa overlap. Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and match to Prosite entry PS00455 Putative AMP-binding domain signature 4 4 20 8 [Reporter]SCO1714 (15H5)_PCR/7984 [Gene]SCO1714/7266 NC_003888 SCI11.03c, possible secreted protein, len: 189 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains a probable N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site, so may be a lipoprotein 1 2 7 11 [Reporter]SCO4624 (11M19)_PCR/2158 [Gene]SCO4624/1967 NC_003888 SCD39.24c, unknown, len: 111 aa 4 4 20 7 [Reporter]SCO3550 (16D5)_PCR/7983 [Gene]SCO3550/7265 NC_003888 SCH5.13, possible helicase, len: 815 aa; similar to many e.g. SW:YPRA_BACSU hypothetical helicase from Bacillus subtilis (749 aa) fasta scores; opt: 1616, z-score: 1799.2, E(): 0, (37.7% identity in 735 aa overlap). Also similar to TR:O06359 (EMBL:Z95436) hypothetical protein from Mycobacterium tuberculosis (771 aa) fasta scores; opt: 2550, z-score: 2840.4, E(): 0, (52.5% identity in 774 aa overlap). Contains Pfam match to entry PF00271 helicase_C, Helicases conserved C-terminal domain, score 35.50, E-value 1.2e-06. 1 2 7 10 [Reporter]SCO2925 (12I19)_PCR/2157 [Gene]SCO2925/1966 NC_003888 SCE19A.25c, probable oxidoreductase subunit, len: 488 aa; similar to many e.g. SW:GLCD_ECOLI (EMBL:L43490), GlcD, Escherichia coli glycolate oxidase subunit (499 aa), fasta scores; opt: 1033 z-score: 1172.9 E(): 0, 38.9% identity in 460 aa overlap. Also similar to SW:DLD1_YEAST (EMBL:X66052), dld, Saccharomyces cerevisiae D-lactate dehydrogenase [cytochrome] precursor (587 aa) (29.4% identity in 435 aa overlap). The N-terminus differs from its homologues and an alternative start codon is present at codon 3 4 4 20 6 [Reporter]SCO3336 (17P1)_PCR/7982 [Gene]SCO3336/7264 NC_003888 SCE7.03, possible hydrolase, len: 193aa; similar to many egs. TR:O07081 (EMBL:U93876) hypothetical protein from Bacillus subtilis (187 aa) fasta scores; opt: 370, z-score: 455.2, E(): 5e-18, (35.1% identity in 188 aa overlap) and SW:ENTB_ECOLI isochorismatase from Escherichia coli (285 aa) fasta scores; opt: 133, z-score: 168.5, E(): 0.046, (29.1% identity in 196 aa overlap). Contains Pfam match to entry PF00857 Isochorismatase, Isochorismatase family. 1 2 7 9 [Reporter]SCO2995 (13E19)_PCR/2156 [Gene]SCO2995/1965 NC_003888 SCE99.02, possible ABC transporter integral membrane protein, len: 318 aa; similar to TR:Q9X7V2 (EMBL:AL049485) Streptomyces coelicolor putative ABC transporter membrane component SC6A5.06, 309 aa; fasta scores: opt: 1393 z-score: 1546.8 E(): 0; 69.0% identity in 300 aa overlap and to SW:RFA1_KLEPN (EMBL:L31775) Klebsiella pneumoniae O-antigen export system permease protein RfbA, 259 aa; fasta scores: opt: 327 z-score: 370.8 E(): 4.1e-13; 26.6% identity in 199 aa overlap. Contains Pfam match to entry PF01061 ABC2_membrane, ABC-2 type transporter and possible hydrophobic membrane spanning regions 4 4 20 5 [Reporter]SCO1058 (18L1)_PCR/7981 [Gene]SCO1058/7263 NC_003888 SCG22.04, possible sugar transport integral membrane protein, len: 275 aa; similar to TR:Q9RUF0 (EMBL:AE001988) Deinococcus radiodurans ABC transporter, permease protein, MalFG family, DR1436 aa; fasta scores: opt: 550 z-score: 629.9 E(): 1.3e-27; 32.9% identity in 283 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 1 2 7 8 [Reporter]SCO3375 (14A19)_PCR/2155 [Gene]SCO3375/1964 NC_003888 SCE94.26c, Lsr2-like protein, len: 111 aa; similar to SW:LSR2_MYCLE surface antigen protein from Mycobacterium tuberculosis (112 aa) fasta scores; opt: 358, z-score: 416.0, E(): 7.4e-16, (55.4% identity in 112 aa overlap). 4 4 20 4 [Reporter]SCO6308 (19H1)_PCR/7980 [Gene]SCO6308/7262 NC_003888 SCIF3.10c, possible hydrolase, len: 333 aa; similar to TR:Q9RJW4 (EMBL:AL117387) Streptomyces coelicolor putative 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase SCF41.05, 292 aa; fasta scores: opt: 496 z-score: 573.9 E(): 2e-24; 36.3% identity in 311 aa overlap. Contains Pfam match to entry PF01557 FAA_hydrolase, Fumarylacetoacetate (FAA) hydrolase family 4 3 2 16 [Reporter]SCO7294 (6P18)_PCR/5581 [Gene]SCO7294/5089 NC_003888 SC5F8.04c, hypothetical protein, len: 434 aa. Similar to Streptomyces coelicolor TR:Q9X9W9 (EMBL:AL096743) putative transport associated protein, SCI7.21 (399 aa), fasta scores opt: 428 z-score: 449.2 E(): 1.5e-17 38.3% identity in 209 aa overlap 1 2 7 7 [Reporter]SCO1516 (15M15)_PCR/2154 [Gene]SCO1516/1963 NC_003888 SCL2.06c, secD, protein-export membrane protein, len: 570 aa; identical to previously sequenced SW:SECD_STRCO (EMBL:X85969) Streptomyces coelicolor protein-export membrane protein SecD, 570 aa and similar to SW:SECD_RHOCA (EMBL:U69979) Rhodobacter capsulatus protein-export membrane protein SecD, 554 aa; fasta scores: opt: 483 z-score: 472.1 E(): 6.6e-19; 28.1% identity in 498 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 20 3 [Reporter]SCP1.87c (20D1)_PCR/7979 [Gene]SCP1.87c/7261 NC_003888 SCP1.87c, possible oxidoreductase, len: 303aa; weakly similar to many eg. TR:O54079 (EMBL:AJ223781) thioredoxin reductase (EC 1.6.4.5) from Staphylococcus aureus (311 aa) fasta scores; opt: 250, z-score: 285.3, E(): 2e-08, 27.3% identity in 311 aa overlap. 4 3 2 15 [Reporter]SCO4405 (7L18)_PCR/5580 [Gene]SCO4405/5088 NC_003888 SC6F11.03c, probable ABC transport system ATP-binding protein, len: 324 aa; similar to SW:DRRA_STRPE (EMBL:M73758) Streptomyces peucetius daunorubicin resistance ATP-binding protein DrrA, 330 aa; fasta scores: opt: 857 z-score: 908.1 E(): 0; 45.3% identity in 322 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 2 7 6 [Reporter]SCO0522 (16I15)_PCR/2153 [Gene]SCO0522/1962 NC_003888 SCF11.02, probable oxidoreductase, len: 262 aa; similar to TR:O54197 (EMBL:AJ000671) Streptomyces clavuligerus clavulanate-9-aldehyde reductase, 247 aa; fasta scores: opt: 501 z-score: 567.7 E(): 3e-24; 39.1% identity in 238 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and prosite match to entry PS00061 Short-chain dehydrogenases/reductases family signature. also similar to Streptomyces coelicolor SCF6.15c, 245 aa; fasta scores: opt: 818 z-score: 805.2 E():0; 56.5% identity in 237 aa overlap 4 4 20 2 [Reporter]SCO2811 (20L21)_PCR/7978 [Gene]SCO2811/7260 NC_003888 SCBAC17F8.02c, possible regulator, len: 220 aa: similar to many from Gram positives eg. TR:Q52517 (EMBL:M18263) MmyJ from the methylenomycin cluster of plasmid SCP1 from Streptomyces coelicolor (111 aa) fasta scores; opt: 242, Z-score: 307.2, 43.878% identity in 98 aa overlap and TR:BAB49625 (EMBL:AP002999) probable regulatory protein from Rhizobium loti (118 aa) fasta scores; opt: 174, Z-score: 225.5, 38.095% identity in 84 aa overlap. 4 3 2 14 [Reporter]SCO6868 (8H18)_PCR/5579 [Gene]SCO6868/5087 NC_003888 SC7F9.20c, unknown, len: 368 aa. 1 2 7 5 [Reporter]SCO0336 (17E15)_PCR/2152 [Gene]SCO0336/1961 NC_003888 SCF12.15c, possible integral membrane protein, len: 369aa; contains possible membrane spanning hydrophobic regions. 4 3 2 13 [Reporter]SCO7144 (9D18)_PCR/5578 [Gene]SCO7144/5086 NC_003888 SC9A4.06, possible ECF sigma factor, len: 245 aa; similar to TR:Q9RDI5 (EMBL:AL136519) Streptomyces coelicolor putative sigma factor SCC57A.13c, 315 aa; fasta scores: opt: 395 z-score: 475.1 E(): 5.3e-19; 35.0% identity in 197 aa overlap and to SW:RPSH_PSEAE (EMBL:L14760) Pseudomonas aeruginosa RNA polymerase sigma-H factor AlgU, 193 aa; fasta scores: opt: 143 z-score: 180.2 E(): 0.014; 26.0% identity in 177 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 1 2 7 4 [Reporter]SCO0402 (18A15)_PCR/2151 [Gene]SCO0402/1960 NC_003888 SCF51.01c, unknown, len: >48 aa,SCF62.28c, possible integral membrane protein, partial CDS, len: >248 aa. Weakly similar in parts to Streptomyces coelicolor TR:O86624 (EMBL: AL031155) putative integral membrane protein SC3A7.23C (272 aa), fasta scores opt: 189 z-score: 228.1 E(): 2.5e-05 31.6% identity in 190 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 3 2 12 [Reporter]SCO2332 (10P14)_PCR/5577 [Gene]SCO2332/5085 NC_003888 SCC53.23, possible hydrolase, len: 259 aa; similar to many e.g. SW:Q59695 (ACOC_PSEPU) acetoin dehydrogenase E2 component from Pseudomonas putida (370 aa) fasta scores; opt: 213, z-score: 235.6, E(): 1.2e-05, 30.5% identity in 249 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. Also contains possible stem loop structure in %' half of CDS. 4 3 2 11 [Reporter]SCO4499 (11L14)_PCR/5576 [Gene]SCO4499/5084 NC_003888 SCD35.06, probable tetR-family regulatory protein, len: 240 aa; similar to TR:O53661 (EMBL:AL021929) Mycobacterium tuberculosis putative regulatory protein MTV034.04, 204 aa; fast scores: opt: 447 z-score: 507.8 E(): 7.5e-21; 41.4% identity in 198 aa overlap and to SW:MTRR_NEIGO (EMBL:Z25797) Neisseria gonorrhoeae regulatory protien MtrR, 210 aa; fasta scores: opt: 164 z-score: 193.8 E(): 0.0023; 28.3% identity in 159 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 34..55 (+3.38 SD) 4 3 2 10 [Reporter]SCO4247 (12H14)_PCR/5575 [Gene]SCO4247/5083 NC_003888 SCD8A.20c, unknown, len: 240 aa. Contains Pfam match to entry PF01476 PG_binding_2, Putative peptidoglycan binding domain 4 3 2 8 [Reporter]SCO5028 (14P10)_PCR/5573 [Gene]SCO5028/5082 NC_003888 SCK7.01, possible ATP-binding protein, len: 441 aa; similar to TR:Q9X792 (EMBL:AL049491) Mycobacterium leprae putative ATP-binding protein MLCB1222.20, 433 aa; fasta scores: opt: 2024 z-score: 2236.8 E(): 0; 72.2% identity in 428 aa overlap. Contains and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 3 2 7 [Reporter]SCO1152 (15L10)_PCR/5572 [Gene]SCO1152/5081 NC_003888 SCG8A.06, possible helicase, len: 854 aa; similar in regions to many eg. TR:CAB59484 (EMBL:AL132648) putative helicase from Streptomyces coelicolor (950 aa) fasta scores; opt: 417, z-score: 463.2, E(): 2.3e-18, 26.5% identity in 755 aa overlap and SW:P47047 (MTR4_YEAST) ATP-dependent RNA helicase DOB1 from Saccharomyces cerevisiae (Baker's yeast) (1073 aa) fasta scores; opt: 335, z-score: 370.4, E(): 3.5e-13, 25.1% identity in 650 aa overlap. Contains Pfam match to entry PF00270 DEAD, DEAD/DEAH box helicase. 4 3 2 6 [Reporter]SCO3905 (16H10)_PCR/5571 [Gene]SCO3905/5080 NC_003888 SCH24.27c, putative membrane protein, len: 101 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains possible hydrophobic membrane spanning regions 4 4 19 22 [Reporter]SCO1331 (1L17)_PCR/7976 [Gene]SCO1331/7259 NC_003888 2SCG61.13, possible multi-domain regulatory protein, len: 780aa; similar to proposed regulatory proteins eg. TR:Q11028 (EMBL:Z75555) probable transcriptional regulatory protein from Mycobacterium tuberculosis (1159 aa) fasta scores; opt: 743, z-score: 848.5, E(): 0, 31.1% identity in 903 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop).Also contains a TTA encoded leucine amino acid at residue 649 (possible target for bldA regulation) which may allow for expression of the protein without the C-terminal domain. 4 4 19 21 [Reporter]SCO5776 (2H17)_PCR/7975 [Gene]SCO5776/7258 NC_003888 SC4H8.15c, gluB, probable glutamate binding protein, len: 278 aa; similar to many members of the periplasmic solute-binding family 3 eg. GLUB_CORGL P48242 glutamate-binding protein precursor from Corynebacterium glutamicum (295 aa), fasta scores; opt: 733 z-score: 768.3 E(): 0, 46.9% identity in 277 aa overlap. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site; also contains PS01039 Bacterial extracellular solute-binding proteins, family 3 signature 1 2 7 3 [Reporter]SCP1.299 (19M11)_PCR/2150 [Gene]SCP1.299/1959 NC_003888 SCP1.298, probable hydratase, len: 274aa; similar to many eg. SW:P77608 (MHPD_ECOLI) 2-keto-4-pentenoate hydratase (EC 4.2.1.-) from Escherichia coli (269 aa) fasta scores; opt: 420, z-score: 525.4, E(): 8.5e-22, 34.3% identity in 245 aa overlap. Contains Pfam match to entry PF01689 Hyratase_decarb, Hydratase/decarboxylase. 4 4 19 19 [Reporter]SCO7205 (4P13)_PCR/7973 [Gene]SCO7205/7257 NC_003888 SC2H12.04, possible hydrolase, len: 286 aa. Highly similar to many including: Moraxella sp. SW:DEH1_MORSP(EMBL:D90422) haloacetate dehalogenase H-1 (EC 3.8.1.3) (294 aa), fasta scores opt: 556 z-score: 678.4 E(): 2.6e-30 35.6% identity in 292 aa overlap and Streptomyces coelicolor: TR:Q9RD12(EMBL:AL133422) putative hydrolase, SCM1.32 (298 aa), fasta scores opt: 653 z-score: 795.7 E(): 0 42.2% identity in 287 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 1 2 7 2 [Reporter]SCO4887 (20E11)_PCR/2149 [Gene]SCO4887/1958 NC_003888 2SCK8.13, probable sugar ABC transporter integral membrane protein, len: 374 aa; similar to TR:CAC14306 (EMBL:AJ276209) Clostridium sticklandii putative permease 1 of an ABC transporter RbsC-1, 354 aa; fasta scores: opt: 623 z-score: 693.9 E(): 4.4e-31; 33.3% identity in 342 aa overlap. Contains Pfam match to entry PF02653 BPD_transp_2 and possible hydrophobic membrane spanning regions 4 4 19 18 [Reporter]SCO7667 (5L13)_PCR/7972 [Gene]SCO7667/7256 NC_003888 SC4C2.02, hypothetical protein, len: 198 aa; similar to TR:Q9RJG7 (EMBL:AL121600) Streptomyces coelicolor hypothetical 26.8 kDa protein SCF76.07, 247 aa; fasta scores: opt: 283 z-score: 348.2 E(): 7.3e-12; 29.1% identity in 179 aa overlap. Contains Pfam match to entry PF01966 HD, HD domain 1 2 6 22 [Reporter]SCO5241 (1E7)_PCR/2147 [Gene]SCO5241/1957 NC_003888 2SC7G11.03c, hypothetical protein, len: 322 aa; similar to TR:O05848 (EMBL:Z95120) Mycobacterium tuberculosis hypothetical 34.5 kDa protein MTCY07D11.08c, 321 aa; fasta scores: opt: 694 z-score: 788.3 E(): 0; 39.9% identity in 328 aa overlap. Contains Pfam match to entry PF00781 DAGKc, Diacylglycerol kinase catalytic domain (presumed) 4 4 19 17 [Reporter]SCO7755 (6H13)_PCR/7971 [Gene]SCO7755/7255 NC_003888 SC5E9.03, conserved hypothetical protein, len: 80aa; similar to others from Streptomyces coelicolor eg. TR:O54206 (EMBL:AJ001206) pepA hypothetical protein from the glycogen metabolism cluster (90 aa) fasta scores; opt: 116, z-score: 182.1, E(): 0.011, 36.3% identity in 80 aa overlap. 1 2 6 21 [Reporter]SCO2776 (2A7)_PCR/2146 [Gene]SCO2776/1956 NC_003888 SCC105.07, accD1, acetyl/propionyl CoA carboxylase, beta subunit, len: 538 aa; similar to TR:O06165 (EMBL:Z95556) Mycobacterium tuberculosis, hypothetical 56.7 kD protein MTCY07A7.08c, 529 aa; fasta scores: opt: 2749 z-score: 3110.3 E(): 0; 76.5% identity in 532 aa overlap and to TR:AAF35258 (EMBL:AF059510) Arabidopsis thaliana 3-methylcrotonyl-CoA carboxylase non-biotinylated subunit (EC 6.4.1.4) MccB, 587 aa; fasta scores: opt: 2185 z-score: 2471.8 E(): 0; 60.4% identity in 540 aa overlap. Contains Pfam match to entry PF01039 Carboxyl_trans, Carboxyl transferase domain 4 4 19 16 [Reporter]SCO5645 (7D13)_PCR/7970 [Gene]SCO5645/7254 NC_003888 SC6A9.22c, unknown, len: 368 aa; similar to hypothetical proteins from many organisms e.g. YLON_BACSU hypothetical 41.6 kd protein (363 aa), fasta scores; opt: 772 z-score: 1018.0 E(): 0, 39.2% identity in 342 aa overlap 1 2 6 20 [Reporter]SCO4648 (3M3)_PCR/2145 [Gene]SCO4648/1955 NC_003888 SCD82.19, rplK, 50S ribosomal protein L11, len: 144 aa; identical to previously sequenced SW:RL11_STRCO (EMBL:D32254) Streptomyces coelicolor 50S ribosomal protein L11 RplK. Contains Pfam match to entry PF00298 Ribosomal_L11, Ribosomal protein L11 and match to Prosite entry PS00359 Ribosomal protein L11 signature 4 4 19 15 [Reporter]SCO2495 (8P9)_PCR/7969 [Gene]SCO2495/7253 NC_003888 SCC82.01, unknownm len: >83 aa,SC7A8.34, possible membrane protein (fragment), len: >541 aa. Contains possible hydrophobic membrane spanning regions 1 2 6 19 [Reporter]SCO6496 (4I3)_PCR/2144 [Gene]SCO6496/1954 NC_003888 SC1E6.05, probable dehydrogenase, len: 629 aa; similar to e.g. Mycobacterium smegmatis TR:O52473 (EMBL:AF038423) NADH dehydrogenase (457 aa), fasta scores; opt: 499 z-score: 263.3 E(): 2.2e-07, 31.2% identity in 417 aa overlap. Contains 6x degenerate repeats of the sequence KNQPGGEP(A /G)= (P-loop) 4 4 19 13 [Reporter]SCO7366 (10H9)_PCR/7967 [Gene]SCO7366/7252 NC_003888 SC9H11.20, unknown, len: 225 aa. Similar to Streptomyces coelicolor TR:O86750(EMBL:AL031035) hypothetical 28.3 kd protein, SC6A9.18C (266 aa), fasta scores opt: 304 z-score: 354.9 E(): 2.6e-12 32.8% identity in 253 aa overlap. 4 3 2 5 [Reporter]SCO4954 (17D10)_PCR/5570 [Gene]SCO4954/5079 NC_003888 2SCK31.14c, unknown, len: 197 aa 1 2 6 18 [Reporter]SCO6349 (5E3)_PCR/2143 [Gene]SCO6349/1953 NC_003888 SC3A7.17, probable transcriptional regulator, len: 361 aa; similar to many of the lacI family e.g. LACI_ECOLI lactose operon repressor (360 aa), fasta scores; opt: 518 z-score: 705.7 E(): 4.6e-32, 33.6% identity in 339 aa overlap. Contains helix-turn-helix motif from aa 27-48 (Score 1760, +5.18 SD), and Pfam matches to entry PF00356 lacI, Bacterial regulatory proteins, lacI family, score 35.20, E-value 1.9e-08 and to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family, score 78.80, E-value 1.1e-19 4 4 19 12 [Reporter]SCO2557 (11D9)_PCR/7966 [Gene]SCO2557/7251 NC_003888 SCC77.24, unknown, len: 274 aa. Similar to Mycobacterium tuberculosis TR:O53477(EMBL:AL021899) hypothetical 30.5 KD protein (280 aa), fasta scores opt: 1044 z-score: 1136.4 E(): 0 61.9% identity in 268 aa overlap. 4 3 2 4 [Reporter]SCO1832 (18P6)_PCR/5569 [Gene]SCO1832/5078 NC_003888 SCI8.17c, possible membrane protein, len: 192 aa. Contains a possible N-terminal signal sequence and a possible membrane spanning hydrophobic domain. 1 2 6 17 [Reporter]SCO6632 (6A3)_PCR/2142 [Gene]SCO6632/1952 NC_003888 SC4G2.06, possible membrane protein, len: 149 aa; start uncertain 4 4 19 11 [Reporter]SCO3950 (12P5)_PCR/7965 [Gene]SCO3950/7250 NC_003888 SCD78.17c, conserved hypothetical protein, len: 308 aa; similar to e.g. TR:O07810 (EMBL:Z97188) from Mycobacterium tuberculosis fasta scores; opt: 503, z-score: 626.7, E(): 1.2e-27, (37.9% identity in 277 aa overlap) and YIDA_ECOLI (EMBL:L10328) Escherichia coli fasta scores; opt: 297, z-score: 407.5, E(): 1.9e-15, (31.6% identity in 282 aa overlap). Also similar to SCD78.27c, approx 9kb upstream (37.9% identity in 285 aa overlap). Contains Pfam match to entry PF00592 DUF3, Domain of Unknown Function 3, score 99.00, E-value 9.5e-26 4 3 2 3 [Reporter]SCO1300 (19L6)_PCR/5568 [Gene]SCO1300/5077 NC_003888 SCBAC36F5.11c, possible exonuclease, len: 999 aa; similar to SW:SBCC_ECOLI (EMBL:X15981) Escherichia coli exonuclease SbcC or B0397, 1048 aa; fasta scores: opt: 363 Z-score: 323.3 bits: 71.4 E(): 2.1e-10; 26.004% identity in 1096 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and a possible coiled-coil region at approx. residues 650..698 1 2 6 16 [Reporter]SCO4846 (6M23)_PCR/2141 [Gene]SCO4846/1951 NC_003888 SC5G8.14, possible integral membrane protein, len: 321aa; weakly similar to many eg. SW:P45417 (YHJD_ERWCH) hypothetical protein from Erwinia chrysanthemi (328 aa) fasta scores; opt: 305, z-score: 344.6, E(): 9.7e-12, 29.7% identity in 283 aa overlap. Contains possible membrane-spanning hydrophobic regions. 4 3 2 2 [Reporter]SCP1.287 (20D6)_PCR/5567 [Gene]SCP1.287/5076 NC_003888 SCP1.287, unknown, len: 122aa; weakly similar to TR:Q9KXJ3 (EMBL:AL357591) hypothetical protein from Streptomyces coelicolor (128 aa) fasta scores; opt: 102, z-score: 146.2, E(): 1.1, 26.7% identity in 120 aa overlap. 1 2 6 15 [Reporter]SCO6969 (7I23)_PCR/2140 [Gene]SCO6969/1950 NC_003888 SC6F7.22, possible integral membrane protein, len: 257 aa. Similar to several proteins of undefined function e.g. Campylobacter jejuni TR:CAB73891(EMBL:AL139078) putative integral membrane protein (254 aa), fasta scores opt: 176 z-score: 216.1 E(): 0.00013 24.4% identity in 254 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 3 1 22 [Reporter]SCO5188 (1D2)_PCR/5565 [Gene]SCO5188/5075 NC_003888 2SC3B6.12, probable ATP-dependent DNA helicase, len: 785 aa; similar to SW:UVRD_ECOLI (EMBL:M87049) Escherichia coli DNA helicase II (EC 3.6.1.-) UvrD, 720 aa; fasta scores: opt: 649 z-score: 632.5 E(): 9.2e-28; 31.6% identity in 643 aa overlap. Contains 2x Pfam matches to entry PF00580 UvrD-helicase, UvrD/REP helicase, Pfam match to entry PF00570 HRDC, HRDC domain and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 3 1 21 [Reporter]SCO4935 (1P22)_PCR/5564 [Gene]SCO4935/5074 NC_003888 SCK13.27, possible integral membrane protein, len: 496 aa; C-terminal region similar to C-terminal region of similar to TR:Q9UX75 (EMBL:Y18930) Sulfolobus solfataricus hypothetical 43.0 kDa protein ORF-C10_044, 418 aa; fasta scores: opt: 332 z-score: 303.7 E(): 2.2e-09; 27.1% identity in 325 aa overlap. Contains 4 possible hydrophobic membrane spanning regions 4 3 1 19 [Reporter]SCO7396 (3H22)_PCR/5562 [Gene]SCO7396/5073 NC_003888 SC10G8.24c. possible secreted protein, len: 445 aa. Weakly similar to Mycobacterium tuberculosis TR:O06598(EMBL:Z95586) hypothetical 48.0 KD protein (446 aa), fasta scores opt: 399 z-score: 416.0 E(): 9.3e-16 28.2% identity in 387 aa overlap. Contains a possible N-terminal signal sequence. 4 3 1 18 [Reporter]SCO6186 (4D22)_PCR/5561 [Gene]SCO6186/5072 NC_003888 SC2G5.07, probable phosphoheptose isomerase, len: 229aa; similar to TR:O25528 (EMBL:AE000596) putative phosphoheptose isomerase from Helicobacter pylori (192 aa) fasta scores; opt: 431, z-score: 495.6, E(): 2.6e-20, (46.9% identity in 147 aa overlap) and SW:LPCA_ECOLI phosphoheptose isomerase from Escherichia coli (192 aa) fasta scores; opt: 408, z-score: 469.8, E(): 7e-19, (41.6% identity in 161 aa overlap). Contains Pfam match to entry PF01380 SIS (Sugar ISomerase), SIS domain, score 52.70, E-value 7.9e-12. 4 3 1 17 [Reporter]SCO6710 (5P18)_PCR/5560 [Gene]SCO6710/5071 NC_003888 SC4C6.20c, possible glycosyl hydrolase, len: 729 aa; weakly similar to part of several glycosyl hydrolases e.g. SW:GUXB_CELFI (EMBL:L02544), CenD, Cellulomonas fimi endoglucanase D precursor (1090 aa), fasta scores; opt: 235 z-score: 236.1 E(): 8.1e-06, 29.5% identity in 285 aa overlap and TR:O30678 (EMBL:AF014950), ChiA, Stenotrophomonas maltophilia chitinase A (700 aa) (28.6% identity in 262 aa overlap). Contains 3x degenerate repeats of DTTAPXTSATVXGTKNADGAYVGSATVTVXAXDEGGSGVXXIE YALGADGAWXAYTXPVXVDXVGEHTVRYRAXDKAGNXAEEKSVXF between aa 146 and 424. Weakly similar to the C-terminal half of TR:O69947 (EMBL:AL023862) S. coelicolor hypothetical protein (1238 aa) (23.5% identity in 737 aa overlap). The upstream ORF SC4C6.21c has significant similarity to the remainder of this hypothetical protein. Contains Pfam match to entry PF00127 copper-bind, Copper binding proteins, plastocyanin/azurin family 4 3 1 16 [Reporter]SCO2164 (6L18)_PCR/5559 [Gene]SCO2164/5070 NC_003888 SC5F7.37c, possible integral membrane efflux protein, len: >822aa; similar to SW:ACRD_ECOLI acriflavin resistance efflux protein from Escherichia coli (1037 aa) fasta scores; opt: 628, z-score: 629.0, E(): 1.1e-27, (22.9% identity in 853 aa overlap). Also similar to TR:O31501 (EMBL:Z99107) hypothetical protein from Bacillus subtilis (1065 aa) fasta scores; opt: 968, z-score: 969.5, E(): 0, (29.4% identity in 874 aa overlap). Contains Pfam match to entry PF00873 ACR_tran, AcrB/AcrD/AcrF family. Also contains possible membrane spanning hydrophobic regions.,SC6G10A.02c, possible integral membrane efflux protein (fragment), len: >253 aa; similar to C-terminal region of SW:CZCA_ALCSP (EMBL:D67044) Alcaligenes sp. cation efflux system protein CzcA, 1063 aa; fasta scores: opt: 474 z-score: 507.7 E(): 7.7e-21; 34.6% identity in 246 aa overlap. Contains Pfam match to entry PF00873 ACR_tran, AcrB/AcrD/AcrF family. Also contains possible hydrophobic membrane spanning regions 4 4 19 10 [Reporter]SCO3264 (13L5)_PCR/7964 [Gene]SCO3264/7249 NC_003888 SCE39.14c, possible gntR-family regulator, len: 243aa; weak similarity to many egs. SW:FARR_ECOLI fatty acyl responsive regulator from Escherichia coli (240 aa) fasta scores; opt: 196, z-score: 243.9, E(): 2.8e-06, (24.3% identity in 243 aa overlap) and SW:KORA_STRLI KorA protein found to control plasmid transfer in the Streptomyces lividans plasmid pIJ101 (241 aa) fasta scores; opt: 158, z-score: 198.3, E(): 0.00098, (22.0% identity in 227 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and Prosite match to PS00043 Bacterial regulatory proteins, gntR family signature. Also contains probable helix-turn-helix (+3.94 SD) 29-50aa 4 4 19 9 [Reporter]SCO1180 (14H5)_PCR/7963 [Gene]SCO1180/7248 NC_003888 SCG11A.11, probable DNA polymerase III beta chain, len: 386 aa; N-terminal region similar to TR:O54191 (EMBL:AL021411) Streptomyces coelicolor putative transcriptional regulator SC7H1.32c, 196 aa; fasta scores: opt: 211 z-score: 244.3 E(): 3.1e-06; 37.2% identity in 196 aa overlap and C-terminal region similar to SW:DP3B_MYCLE (EMBL:L39923) Mycobacterium leprae DNA polymerase III, beta chain (EC 2.7.7.7) DnaN, 399 aa; fasta scores: opt: 285 z-score: 322.3 E(): 1.4e-10; 30.7% identity in 358 aa overlap. Contains match in N-terminal region to Pfam entry PF00376 merR, Bacterial regulatory proteins, merR family and C-terminal region to Pfam entry PF00712 DNA_pol3_beta, DNA polymerase III beta subunit. Contains a possible helix-turn-helix motif at residues 21..42 (+2.82 SD) 1 2 6 14 [Reporter]SCO5943 (8E23)_PCR/2139 [Gene]SCO5943/1949 NC_003888 SC7H1.13, probable hydrolase, len: 261; weak similarity to several diverse hydrolases eg. TODF_PSEPU P23133 2-hydroxy-6-oxo-2,4-heptadienoate hydrolase (276 aa), fasta scores; opt: 196 z-score: 344.3 E(): 5.5e-12, 30.7% identity in 270 aa overlap 4 4 19 8 [Reporter]SCO1467 (15D5)_PCR/7962 [Gene]SCO1467/7247 NC_003888 SCL6.24c, hypothetical protein, len: 491 aa; similar to TR:CAB71206 (EMBL:AL138538) Streptomyces coelicolor hypothetical 47.0 kD protein SC6D10.11, 452 aa; fasta scores: opt: 835 z-score: 861.3 E(): 0; 37.6% identity in 434 aa overlap 1 2 6 13 [Reporter]SCO6063 (9A23)_PCR/2138 [Gene]SCO6063/1948 NC_003888 SC9B1.10, probable ABC transport permease, len: 237aa; similar to many eg. TR:O69723 (EMBL:AL022121) transport system permease from Mycobacterium tuberculosis (229 aa) fasta scores; opt: 436, z-score: 523.9, E(): 7.4e-22, (36.4% identity in 214 aa overlap) and SW:PROZ_BACSU glycine betaine/L-proline transport permease from Bacillus subtilis (223 aa) fasta scores; opt: 291, z-score: 353.3, E(): 2.3e-12, (29.1% identity in 199 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. 4 4 19 7 [Reporter]SCO1588 (16P1)_PCR/7961 [Gene]SCO1588/7246 NC_003888 SCI35.10, probable integral membrane protein, len: 320 aa; highly similar to a hypothetical protein from B. subtilis TR:P96668 (EMBL:AB001488) YdeK protein, 287 aa; fasta scores; opt: 624 z-score: 893.6 E(): 0, 40.3% identity in 293 aa overlap 1 2 6 12 [Reporter]SCO2734 (10M19)_PCR/2137 [Gene]SCO2734/1947 NC_003888 SCC57A.05, possible LysR-family transcriptional regulator, len: 344 aa. Similar to several other regulators including: Streptomyces lividans TR:Q9X9S5 (EMBL:Y18818) transcriptional regulator (LysR-family), found adjacent to the actinorhodin biosynthetic cluster (307 aa), fasta scores opt: 275 z-score: 303.4 E(): 1.6e-09 31.3% identity in 336 aa overlap. Also similar to Streptomyces coelicolor regulators: TR:Q9X9U9 (EMBL:Y18817) transcriptional regulator of LysR family (307 aa), fasta scores opt: 274 z-score: 302.6 E(): 1.8e-09 31.3% identity in 336 aa overlap and (EMBL:AL136058) putative LysR-family transcriptional regulator, SCE20.14 (297 aa), fasta scores opt: 509 z-score: 460.1 E(): 3.3e-20 37.5% identity in 323 aa overlap. Contains a Prosite hit to PS00044 Bacterial regulatory proteins, lysR family signature and a Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family with the putative helix-turn-helix motif situated between residues 16..37 (+2.63 SD). 4 4 19 6 [Reporter]SCO1547 (17L1)_PCR/7960 [Gene]SCO1547/7245 NC_003888 SCL11.03c, possible anthranilate synthase, len: 353 aa; similar to SW:TRPE_THEMA (EMBL:X74075) Thermotoga maritima Anthranilate synthase component I (EC 4.1.3.27) TrpE, 457 aa; fasta scores: opt: 459 z-score: 510.1 E(): 5.4e-21; 37.2% identity in 285 aa overlap. Contains Pfam match to entry PF00425 chorismate_bind, chorismate binding enzyme 1 2 6 11 [Reporter]SCO4617 (11I19)_PCR/2136 [Gene]SCO4617/1946 NC_003888 SCD39.17c, possible ATP-binding protein, len: 490 aa. Contains match to Prosite entry and PS00017 ATP/GTP-binding site motif A (P-loop) 4 4 19 5 [Reporter]SCO3332 (18H1)_PCR/7959 [Gene]SCO3332/7244 NC_003888 SCE68.30c, possible membrane protein, len: 238 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains hydrophobic, possible membrane-spanning regions. Alternative start codons are present at codons 10 and 11. 1 2 6 10 [Reporter]SCO4298 (12E19)_PCR/2135 [Gene]SCO4298/1945 NC_003888 SCD95A.31, probable carboxylesterase, len: 513 aa; similar to TR:Q9Z545 (EMBL:AL035212) Streptomyces coelicolor putative carboxylesterase SC9B2.14, 502 aa; fasta scores: opt: 1093 z-score: 1041.3 E(): 0; 44.6% identity in 511 aa overlap and to SW:PNBA_BACSU (EMBL:U06089) Bacillus subtilis para-nitrobenzyl esterase (EC 3.1.1.-) PnbA, 489 aa; fasta scores: opt: 823 z-score: 786.2 E(): 0; 38.1% identity in 501 aa overlap. Contains Pfam match to entry PF00135 COesterase, Carboxylesterases and match to Prosite entry PS00122 Carboxylesterases type-B serine active site 4 4 19 4 [Reporter]SCO1987 (19D1)_PCR/7958 [Gene]SCO1987/7243 NC_003888 SC7H2.01, partial CDS, unknown, len: >90aa; similar to the C-terminal region of several hypothetical proteins both prokaryote and eukaryote egs. SW:Y709_SYNY3 hypothetical protein from Synechocystis sp. strain PCC6803 (130 aa) fasta scores; opt: 100, z-score: 145.9, E(): 0.84, (34.9% identity in 83 aa overlap) and SW:UK14_CAPHI from Capra hircus (goat) (137 aa) fasta scores; opt: 96, z-score: 140.4, E(): 1.7, (34.6% identity in 81 aa overlap).,SC3C9.22, conserved hypothetical protein (fragment), len: >115 aa; similar to N-terminal region of TR:BAB48518 (EMBL:AP002996) Rhizobium loti MLL1058 protein, 148 aa; fasta scores: opt: 175 Z-score: 218.8 bits: 46.1 E(): 0.00014; 40.789% identity in 76 aa overlap. Contains Pfam match to entry PF01042 UPF0076, YjgF family 1 2 6 9 [Reporter]SCO3321 (13A19)_PCR/2134 [Gene]SCO3321/1944 NC_003888 SCE68.19c, possible redoxin, len: 101 aa; similar to TR:O69271 (EMBL:Y09572), NrdH, Corynebacterium ammoniagenes NrdH-redoxin from a ribonucleotide reductase operon (75 aa), fasta scores; opt: 96 z-score: 145.6 E(): 0.88, 27.6% identity in 76 aa overlap. Also similar to SW:YV35_MYCTU (EMBL:Z77162) Mycobacterium tuberculosis hypothetical protein (97 aa) (33.3% identity in 75 aa overlap). Cys32 and Cys35 are conserved in similar proteins 4 4 19 3 [Reporter]SCO3999 (19P21)_PCR/7957 [Gene]SCO3999/7242 NC_003888 2SC10A7.03, possible lipoprotein, len: 376 aa. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 4 3 1 15 [Reporter]SCO2675 (7H18)_PCR/5558 [Gene]SCO2675/5069 NC_003888 SC6D10.18c, unknown, len: 238 aa 1 2 6 8 [Reporter]SCO4962 (14M15)_PCR/2133 [Gene]SCO4962/1943 NC_003888 2SCK31.22, probable amino acid deaminase, len: 416 aa; similar to SW:THD2_ECOLI (EMBL:M21312) Escherichia coli threonine deaminase TdcB, 409 aa; fasta scores: opt: 899 z-score: 948.9 E(): 0; 46.0% identity in 309 aa overlap. Contains Pfam match to entry PF00291 PALP, Pyridoxal-phosphate dependent enzyme and match to Prosite entry PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site 4 4 19 2 [Reporter]SCO5085 (20H21)_PCR/7956 [Gene]SCO5085/7241 NC_003888 SCBAC28G1.11, actII-4, actinorhodin cluster activator protein, len: 255 aa; identical to previously sequenced SW:AC24_STRCO (EMBL:M64683) Streptomyces coelicolor actinorhodin operon activator protein ActII-4, 255 aa. Contains a leucine TTA codon, possible target for bldA regulation 4 3 1 14 [Reporter]SCO6864 (8D18)_PCR/5557 [Gene]SCO6864/5068 NC_003888 SC7F9.16c, unknown, len: 596 aa. Rich in the amino acid Ala. 1 2 6 7 [Reporter]SCO1148 (15I15)_PCR/2132 [Gene]SCO1148/1942 NC_003888 SCG8A.02, probable ABC transporter, len: 593aa; similar to many eg. TR:CAB76002 (EMBL:AL157916) putative ABC transporter from Streptomyces coelicolor (583 aa) fasta scores; opt: 1624, z-score: 1736.4, E(): 0, 46.8% identity in 575 aa overlap. Contains Pfam match to entry PF00664 ABC_membrane, ABC transporter transmembrane region, Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). Also contains possible membrane-spanning hydrophobic regions. 4 4 18 22 [Reporter]SCO1109 (1H17)_PCR/7954 [Gene]SCO1109/7240 NC_003888 2SCG38.02, probable oxidoreductase, len: 359 aa; similar to SW:NEMA_ECOLI (EMBL:D86931) Escherichia coli N-ethylmaleimide reductase (EC 1.-.-.-) NemA, 365 aa; fasta scores: opt: 509 z-score: 564.1 E(): 5.6e-24; 32.1% identity in 364 aa overlap. Contains Pfam match to entry PF00724 oxidored_FMN, NADH:flavin oxidoreductase / NADH oxidase family 4 3 1 13 [Reporter]SCO5856 (9P14)_PCR/5556 [Gene]SCO5856/5067 NC_003888 SC9B10.23, unknown ATP/GTP binding protein (putative membrane protein), len: 294 aa; contains PS00017 ATP/GTP-binding site motif A (P-loop). Contains possible hydrophobic membrane spanning regions 1 2 6 6 [Reporter]SCO0943 (16E15)_PCR/2131 [Gene]SCO0943/1941 NC_003888 SCM10.31, unknown, len: 171 aa 4 3 1 12 [Reporter]SCO2328 (10L14)_PCR/5555 [Gene]SCO2328/5066 NC_003888 SCC53.19, possible dipeptidase, len: 218aa; similar to TR:Q9R6S5 (EMBL:Y18874) hypothetical 26.3 kD protein from Synechococcus PCC6301 (232 aa) fasta scores; opt: 258, z-score: 323.7, E(): 1.5e-10, 30.8% identity in 227 aa overlap and SW:Q06241 (VANX_ENTFC) D-alanyl-D-alanine dipeptidase (vancomycin resistance protein) (EC 3.4.13.-) from Enterococcus faecium (202 aa) fasta scores; opt: 173, z-score: 220.5, E(): 8.3e-05, 33.1% identity in 136 aa overlap. Contains Pfam match to entry PF01427 Peptidase_M45, D-ala-D-ala dipeptidase. 1 2 6 5 [Reporter]SCO1176 (17A15)_PCR/2130 [Gene]SCO1176/1940 NC_003888 SCG11A.07c, probable dihydroxy-acid dehydratase,len: 596 aa; highly similar to TR:CAB46389 (EMBL:AL096743) Streptomyces coelicolor putative dihydroxy-acid dehydratase IlvD, 576 aa; fasta scores opt: 1203 z-score: 1368.5 E(): 0; 53.2% identity in 583 aa overlap and to SW:ILVD_BACSU (EMB:L77246) Bacillus subtilis dihydroxy-acid dehydratase (EC 4.2.1.9) (Dad) (vegetative protein 110) (VEG110) 557 aa; fasta scores: opt: 695 z-score: 790.6 E(): 0; 35.9% identity in 571 aa overlap. Contains match to Pfam entry PF00920 ILVD_EDD, Dehydratase family and match to Prosite entry PS00886 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1 4 3 1 11 [Reporter]SCO4105 (11H14)_PCR/5554 [Gene]SCO4105/5065 NC_003888 SCD17.09, unknown, len: 106 aa. Highly similar to Streptomyces coelicolor TR:CAB40321 (EMBL; AL049573) hypothetical 10.8 KD protein SCE39.13C (99 aa), fasta scores opt: 192 z-score: 253.4 E(): 9.3e-07 43.0% identity in 100 aa overlap. 4 3 1 10 [Reporter]SCO4243 (12D14)_PCR/5553 [Gene]SCO4243/5064 NC_003888 SCD8A.16c, possible secreted protein, len: 190 aa. Contains possible N-terminal region signal peptide sequence 4 3 1 9 [Reporter]SCO3121 (13P10)_PCR/5552 [Gene]SCO3121/5063 NC_003888 SCE41.30, unknown, len: 42 aa. Doubtful CDS, TBparse predicted 4 3 1 8 [Reporter]SCO1897 (14L10)_PCR/5551 [Gene]SCO1897/5062 NC_003888 SCI7.15, probable transcriptional regulator, len: 258 aa; similar to many e.g. SW:GATR_ECOLI galacticol utilisation operon repressor from Escherichia coli (259 aa) fasta scores; opt: 399, z-score: 477.4, E(): 2.9e-19, (33.8% identity in 237 aa overlap). Contains Pfam match to entry PF00455 deoR, Bacterial regulatory proteins, deoR family. Also contains a helix-turn-helix motif (+3.53 SD) 26-47aa. 4 3 1 7 [Reporter]SCO1764 (15H10)_PCR/5550 [Gene]SCO1764/5061 NC_003888 SCI51.04, hypothetical protein, len: 253 aa; unknown function, shows weak similarity to TR:P94389 (EMBL:D50453), YcgL, Bacillus subtilis hypothetical protein (260 aa), fasta scores; opt: 177 z-score: 222.1 E(): 5.1e-05, 28.1% identity in 196 aa overlap. There is some overlap with the C-terminus of SCI51.5c and weak similarity with SCI51.5c (250 aa) (29.6% identity in 233 aa overlap) 4 3 1 6 [Reporter]SCO1451 (16D10)_PCR/5549 [Gene]SCO1451/5060 NC_003888 SCL6.08c, probable endoglucanase precursor, len: 383 aa; similar to SW:GUN1_STRSQ (EMBL:L03218) Streptomyces sp. endoglucanase 1 precursor (EC 3.2.1.4) CasA, 359 aa; fasta scores: opt: 1042 z-score: 1000.3 E(): 0; 50.6% identity in 324 aa overlap. Contains Pfam match to entry PF01341 Glyco_hydro_6, Glycosyl hydrolases family 6 and possible N-terminal region signal peptide sequence 4 4 18 21 [Reporter]SCO0670 (2D17)_PCR/7953 [Gene]SCO0670/7239 NC_003888 SCF91.30, glpD, glycerol-3-phosphate dehydrogenase (EC 1.1.99.5), len: 527 aa. Highly similar to many prokaryotic and eukaryotic glycerol-3-phosphate dehydrogenases including: Mycobacterium tuberculosis SW:GLPD_MYCTU (EMBL:Z70692) (516 aa), fasta scores opt: 1648 z-score: 1727.9 E():0 52.6% identity in 513 aa overlap and Caenorhabditis elegans SW:GPDM_CAEEL(EMBL:Z73906) (722aa), fasta score opt: 668 z-score: 700.9 E(): 1.2e-31 33.9% identity in 537 aa overlap. Contains a Pfam match to entry PF01224 FAD_Gly3P_dh, FAD-dependent glycerol-3-phosphate dehydrogenase and a Prosite hit to PS00978 FAD-dependent glycerol-3-phosphate dehydrogenase signature 2. 4 4 18 20 [Reporter]SCO6803 (3P13)_PCR/7952 [Gene]SCO6803/7238 NC_003888 SC1A2.12, possible acetyltransferase, len: 181 aa, Similar to Pseudomonas syringae (pv. syringae) SW:TTR_PSESY(EMBL:X17150) acetyltransferase (EC 2.3.1.-) involved in resistance to tabtoxin (177 aa), fasta scores opt: 469 z-score: 572.3 E(): 1.8e-24 47.0% identity in 168 aa overlap. Contains a Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 1 2 6 4 [Reporter]SCO0687 (18M11)_PCR/2129 [Gene]SCO0687/1939 NC_003888 SCF15.08c. probable oxidoreductase, len: 264 aa. Similar to many including: Escherichia coli SW:UCPA_ECOLI (EMBL:AE000330) oxidoreductase UcpA (EC 1.-.-.-) (285 aa), fasta scores opt: 344 z-score: 389.1 E(): 2.7e-14 33.2% identity in 268 aa overlap and Streptomyces coelicolor TR:CAB55718 (EMBL:AL117387) probable oxidoreductase SCF41.15 (263 aa), fasta scores opt: 418 z-score: 471.2 E(): 7.1e-19 36.5% identity in 255 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus. In addition to a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature 4 4 18 19 [Reporter]SCO6210 (4L13)_PCR/7951 [Gene]SCO6210/7237 NC_003888 SC2G5.31, unknown, len: 133 aa; probably member of a family of transthyretin-like proteins, similar to many eg. SW:YEDX_ECOLI hypothetical transthyretin-like protein fromEscherichia coli (137 aa) fasta scores; opt: 149, z-score:198.9, E(): 0.00086, (40.9% identity in 127 aa overlap). 1 2 6 3 [Reporter]SCP1.311c (19I11)_PCR/2128 [Gene]SCP1.311c/1938 NC_003888 SCP1.311c, possible short-chain oxidoreductase, len: 271aa; similar to many proposed oxidoreductase eg. SW:P37959 (YUSZ_BACSU) hypothetical oxidoreductase from Bacillus subtilis (280 aa) fasta scores; opt: 441, z-score: 502.6, E(): 1.6e-20, 33.6% identity in 274 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00687 Aldehyde dehydrogenases glutamic acid active site. 4 4 18 18 [Reporter]SCO2374 (5H13)_PCR/7950 [Gene]SCO2374/7236 NC_003888 SC4A7.02, probable transcriptional regulator, len: 227 aa; similar to SW:TCMR_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C transcriptional repressor TcmR, 226 aa; fasta scores: opt: 594 z-score: 727.4 E(): 0; 46.5% identity in 215 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a possible helix-turn-helix motif at residues 49..70 (+3.84 SD) 1 2 6 2 [Reporter]SCO1295 (20A11)_PCR/2127 [Gene]SCO1295/1937 NC_003888 SCBAC36F5.06, probable AsnC-family transcriptional regulatory protein, len: 163 aa; similar to SW:LRP_RHIME (EMBL:AF193023) Rhizobium meliloti leucine-responsive regulatory protein Lrp, 156 aa; fasta scores: opt: 285 Z-score: 357.6 bits: 72.4 E(): 2.6e-12; 33.333% identity in 147 aa overlap. Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family and possible helix-turn-helix motif at residues 24..45 (+2.96 SD) 4 4 18 17 [Reporter]SCO6549 (6D13)_PCR/7949 [Gene]SCO6549/7235 NC_003888 SC5C7.34, unknow, len: 314 aa; highly His-rich in N-terminus 1 2 5 21 [Reporter]SCO1489 (2M3)_PCR/2124 [Gene]SCO1489/1936 NC_003888 SC9C5.13, bldD, putative DNA-binding protein, len: 167 aa; identical to previously sequenced TR:O52732 (EMBL:AF045549) Streptomyces coelicolor putative DNA binding protein BldD, 167 aa. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix 4 4 18 16 [Reporter]SCO6781 (7P9)_PCR/7948 [Gene]SCO6781/7234 NC_003888 SC6A5.30c, possible regulatory protein, len: 404 aa; varying levels of similarity to hypothetical proteins and proposed regulators e.g. TR:P95251 (EMBL:Z84498) hypothetical protein from Mycobacterium tuberculosis (406 aa) fasta scores; opt: 319, z-score: 348.9, E(): 3.9e-12, (29.6% identity in 372 aa overlap) and TR:O24741 (EMBL:AB001683) receptor for an extracellular regulatory factor from Streptomyces sp. strain FRI-5 (221 aa) fasta scores; opt: 165, z-score: 187.4, E(): 0.0039, (43.9% identity in 82 aa overlap). Contains two Pfam matches to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a Prosite match to PS01081 Bacterial regulatory proteins, tetR family signature. Also contains 2 probable helix-turn-helix (+4.73 SD) 246-267aa and (+4.37 SD) 53-73 aa 1 2 5 20 [Reporter]SCO4703 (3I3)_PCR/2123 [Gene]SCO4703/1935 NC_003888 SCD31.28, rplD, 50S ribosomal protein L4, len: 219 aa; highly similar to SW:RL4_BACST (EMBL:X67014) Bacillus stearothermophilus 50S ribosomal protein L4 RplD, 207 aa; fasta scores: opt: 563 z-score: 669.3 E(): 7.5e-30; 44.1% identity in 202 aa overlap. Contains Pfam match to entry PF00573 Ribosomal_L4, Ribosomal protein L4/L1 family 4 4 18 14 [Reporter]SCO5500 (9H9)_PCR/7946 [Gene]SCO5500/7233 NC_003888 SC8D9.12, putative membrane protein, len: 79 aa. Contains possible hydrophobic membrane spanning regions 1 2 5 19 [Reporter]SCO6904 (4E3)_PCR/2122 [Gene]SCO6904/1934 NC_003888 SC1B2.10, possible membrane protein, len: 336 aa.Contains a possible membrane spanning hydrophobic domain. Rich in the amino acid Threonine 4 4 18 13 [Reporter]SCO7362 (10D9)_PCR/7945 [Gene]SCO7362/7232 NC_003888 SC9H11.16c, oxidoreductase, len: 346 aa. Previously sequenced: Streptomyces coelicolor TR:BAA82700EMBL:AB017438) Orf3 (346 aa). Also highly similar to Corynebacterium sp. ST-10 TR:Q9ZN85(EMBL:AB020760) phenylacetaldehyde reductase (385 aa), fasta scores opt: 1382 z-score: 1438.5 E(): 0 65.2% identity in 316 aa overlap and many alcohol dehydrogenases e.g. from Sulfolobus solfataricus SW:ADH1_SULSO(EMBL:S51211) NAD-dependent alcohol dehydrogenase (EC 1.1.1.1) (347 aa), fasta scores opt: 595 z-score: 624.4 E(): 2.5e-27 32.7% identity in 355 aa overlap. Contains a Prosite hit to PS00059 Zinc-containing alcohol dehydrogenases signature and a Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases. 4 3 1 5 [Reporter]SCO0475 (17P6)_PCR/5548 [Gene]SCO0475/5059 NC_003888 SCF76.15c, probable ABC transporter protein, integral membrane subunit, len: 283 aa. Similar to many e.g. Escherichia coli SW:ZNUB_ECOLI (EMBL; AE000280) high-affinity zinc uptake system membrane protein ZnuB (261 aa), fasta scores opt: 442 z-score: 483.2 E(): 1.5e-19 32.5% identity in 246 aa overlap. Contains a Pfam match to entry PF00950 ABC-3, ABC 3 transport family and multiple possible membrane spanning hydrophobic domains. 1 2 5 18 [Reporter]SCO6344 (5A3)_PCR/2121 [Gene]SCO6344/1933 NC_003888 SC3A7.12, probable secreted amidase, len: 535 aa; similar to many (except N-terminal signal peptide sequence of this CDS) e.g. SW:GATA_STRCO (EMBL:AL035569) Streptomyces coelicolor glutamyl-tRNA(Gln) amidotransferase subunit A GatA or SC8D9.11, 497 aa; fasta scores: opt: 631 Z-score: 664.3 E(): 2.4e-29; 33.932% identity in 501 aa overlap. Contains N-terminal signal sequence 4 4 18 12 [Reporter]SCO4375 (11P5)_PCR/7944 [Gene]SCO4375/7231 NC_003888 SCD10.07, possible marR-family regulatory protein, len: 164 aa; similar to TR:CAB87337 (EMBL:AL163672) Streptomyces coelicolor putative regulatory protein SCE50.15, 168 aa; fasta scores: opt: 226 z-score: 281.6 E(): 3.3e-08; 39.3% identity in 135 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 4 3 1 4 [Reporter]SCO3653 (18L6)_PCR/5547 [Gene]SCO3653/5058 NC_003888 SCH10.31c, probable two-component system regulator, len: 248 aa; similar to many e.g. TR:Q53894 (EMBL:U51332), AbsA2, Streptomyces coelicolor two-component system regulator homolog involved in negative regulation of antibiotic synthesis (222 aa), fasta scores; opt: 583 z-score: 647.2 E(): 9.8e-29, 45.0% identity in 222 aa overlap. Similar to many other putative two-component system regulators from Streptomyces coelicolor e.g. SCH10.18c, probable two-component regulator (228 aa) (56.2% identity in 226 aa overlap). Contains Pfam matches to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 80.40, E-value 3.7e-20 and entry PF00072 response_reg, Response regulator receiver domain, score 127.40, E-value 2.5e-34. Contains probable helix-turn-helix motif at aa 196-217 (Score 1378, +3.88 SD) 1 2 5 17 [Reporter]SCO7069 (5M23)_PCR/2120 [Gene]SCO7069/1932 NC_003888 SC4G1.35c, possible secreted hydrolase, len: 327 aa; similar to SW:GUXB_CELFI (EMBL:L38827) Cellulomonas fimi exoglucanase B precursor (EC 3.2.1.91) CbhB, 1090 aa; fasta scores: opt: 384 z-score: 407.1 E(): 3.3e-15; 33.9% identity in 292 aa overlap and to TR:O54183 (EMBL:AL021411) Streptomyces coelicolor chitinase SC7H1.24, 765 aa; fasta scores: opt: 416 z-score: 443.1 E(): 3.2e-17; 40.3% identity in 201 aa overlap. Contains 2x Pfam matches to entry PF00041 fn3, Fibronectin type III domain and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 4 4 18 11 [Reporter]SCO3946 (12L5)_PCR/7943 [Gene]SCO3946/7230 NC_003888 SCD78.13, cydB, cytochrome oxidase subunit II, len: 334 aa; similar to CYDB_ECOLI (EMBL:J03939) cytochrome D ubiquinol oxidase subunit II from Escherichia coli (379 aa) fasta scores: opt: 674, z-score: 1092.0, E():0 (37.3% identity in 367 aa overlap) and CYDB_BACSU (EMBL:D83026) cytochrome D ubiquinol oxidase subunit II from Bacillus subtilis (338 aa), fasta scores; opt: 1041, z-score: 1431.1, E():0, (47.1% identity in 329 aa overlap). Contains possible membrane spanning regions 4 3 1 3 [Reporter]SCP1.25c (19H6)_PCR/5546 [Gene]SCP1.25c/5057 NC_003888 SCP1.25c, unknown, len: 354aa; 1 2 5 16 [Reporter]SCO4842 (6I23)_PCR/2119 [Gene]SCO4842/1931 NC_003888 SC5G8.10c, possible oxidoreductase, len: 457aa; similar to many of undefined function eg. SW:P43167 (YMP4_STRCO) hypothetical protein from Streptomyces coelicolor (367 aa)fasta scores; opt: 1349, z-score: 1513.9, E(): 0, 58.1% identity in 375 aa overlap. Contains Pfam match to entry PF01565 FAD_binding_4, FAD binding domain. 3 3 23 22 [Reporter]SCO4940 (1K22)_PCR/5544 [Gene]SCO4940/5056 NC_003888 SCK13.32, possible tetR-family transcriptional regulator, len: 200 aa; similar to TR:Q9KZX2 (EMBL:AL353815) Streptomyces coelicolor putative transcriptional regulator SCD6.32c, 200 aa; fasta scores: opt: 183 z-score: 230.1 E(): 2.8e-05; 28.3% identity in 159 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 33..54 (+4.53 SD) 1 2 5 15 [Reporter]SCO7440 (7E23)_PCR/2118 [Gene]SCO7440/1930 NC_003888 SC6D11.36c, possible hydrolase, len: 314 aa. Similar to several including Moraxella sp. SW:DEH1_MORSP (EMBL:D90422) haloacetate dehalogenase H-1 (EC 3.8.1.3) (294 aa). fasta scores opt: 356 z-score: 414.9 E(): 1.1e-15 27.9% identity in 297 aa overlap and Streptomyces coelicolor TR:O86546 (EMBL:AL031350) putative hydrolase, SC1F2.09C (309 aa), fasta scores opt: 498 z-score: 577.2 E(): 9.9e-25 35.2% identity in 307 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 3 3 23 21 [Reporter]SCO1438 (2G22)_PCR/5543 [Gene]SCO1438/5055 NC_003888 SC6D7.01, hisG, ATP phosphoribosyltransferase (EC 2.4.2.17), partial CDS, len: > 104 aa. Highly similar to many ATP phosphoribosyltransferases including: Escherichia coli SW:HIS1_ECOLI(EMBL:X13462) (299 aa), fasta scores opt: 167 z-score: 232.6 E(): 1.4e-05 34.3% identity in 102 aa overlap and Mycobacterium tuberculosis SW:HIS1_MYCTU(EMBL:Z97559) (284 aa), fasta scores opt: 335 z-score: 457.3 E(): 4.3e-18 47.1% identity in 104 aa overlap.,SC6D7A.01c, hisG, ATP phosphoribosyltransferase (fragment), len: >219 aa;similar to SW:HIS1_SULSO (EMBL:U82227) Sulfolobus solfataricus ATP phosphoribosyltransferase (EC 2.4.2.17) HisG, 290 aa; fasta scores: opt: 370 z-score: 450.1 E(): 1.6e-17; 35.0% identity in 220 aa overlap and to SW:HIS1_MYCTU (EMBL:Z97559) Mycobacterium tuberculosis ATP phosphoribosyltransferase (EC 2.4.2.17), 284 aa; fasta scores: opt: 768 z-score: 924.9 E(): 0; 53.4% identity in 219 aa overlap. Contains Pfam match to entry PF01634 HisG, ATP phosphoribosyltransferase 3 3 23 20 [Reporter]SCO7389 (3C22)_PCR/5542 [Gene]SCO7389/5054 NC_003888 SC10G8.16, possible oxidoreductase, len: 292 aa. Similar to many including: Streptomyces clavuligerus TR:O54197(EMBL:AJ000671) clavulanate-9-aldehyde reductase (247 aa), fasta scores opt: 352 z-score: 389.3 E(): 2.9e-14 33.0% identity in 221 aa overlap and Streptomyces coelicolor TR:CAB59666(EMBL:AL132674) probable oxidoreductase, SCE87.21c (297 aa), fasta scores opt: 931 z-score: 1011.4 E():0 52.8% identity in 286 aa overlap. Contains a Prosite hit PS00061 Short-chain dehydrogenases/reductases family signature and a Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 3 3 23 19 [Reporter]SCO7613 (4O18)_PCR/5541 [Gene]SCO7613/5053 NC_003888 SC2H2.11c, possible integral membrane protein, len: 818 aa. Contains possible hydrophobic membrane spanning regions and 4x degenerate repeat unit: SRTGG 3 3 23 18 [Reporter]SCO6705 (5K18)_PCR/5540 [Gene]SCO6705/5052 NC_003888 SC4C6.15c, probable secreted protein, len: 325 aa; similar to TR:O30567 (EMBL:AF011544), yecC, Bacillus subtilis hypothetical protein (331 aa), fasta scores; opt: 278 z-score: 312.3 E(): 4.6e-10, 25.9% identity in 305 aa overlap. Also similar to yerB, which is identical to yecC. Contains a probable N-terminal signal sequence 3 3 23 17 [Reporter]SCO7557 (6G18)_PCR/5539 [Gene]SCO7557/5051 NC_003888 SC5F1.11, possible sugar transport integral membrane protein, len: 307 aa; similar to TR:Q9X9R5 (EMBL:AJ009797) Streptomyces reticuli CebG protein, 275 aa; fasta scores: opt: 1165 z-score: 1331.6 E(): 0; 62.8% identity in 274 aa overlap and to TR:CAC10106 (EMBL:AL442165) Streptomyces coelicolor putative cellobiose transport permease 2SCC13.05, 303 aa; fasta scores: opt: 1236 z-score: 1261.9 E(): 0; 61.1% identity in 296 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Also contains possible hydrophobic membrane spanning regions 3 3 23 16 [Reporter]SCO2670 (7C18)_PCR/5538 [Gene]SCO2670/5050 NC_003888 SC6D10.13c, probable O-methyltransferase, len: 324 aa; similar to TR:O30199 (EMBL:AE001104) Archaeoglobus fulgidus L-isoaspartyl protein carboxyl methyltransferase AF0036, 216 aa; fasta scores: opt: 373 z-score: 428.9 E(): 1.8e-16; 36.8% identity in 209 aa overlap and to SW:PIMT_METJA (EMBL:U67474) Methanococcus jannaschii protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), 215 aa; fasta scores: opt: 356 z-score: 407.3 E(): 2.8e-15; 42.1% identity in 140 aa overlap. Contains Pfam match to entry PF01135 PCMT, Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) and match to Prosite entry PS01279 Protein-L-isoaspartate(D-aspartate) O-methyltransferase signature 4 4 18 10 [Reporter]SCO3067 (13H5)_PCR/7942 [Gene]SCO3067/7229 NC_003888 SCE25.08c, putative anti anti sigma factor, len: 125 aa; identical to previously sequenced TR:CAB55835 (EMBL:AJ249581) Streptomyces coelicolor A3(2) putative anti anti sigma factor, 125 aa. Contains Pfam match to entry PF01740 SpoIIAA, SpoIIAA family 4 4 18 9 [Reporter]SCO0417 (14D5)_PCR/7941 [Gene]SCO0417/7228 NC_003888 SCF51.16c, hypothetical protein, len: 248 aa; similar to TR:P74164 (EMBL:D90912) Synechocystis sp. (strain PCC 6803) hypothetical 33.9 KD protein, 300 aa; fasta scores: opt: 361 z-score: 423.5 E(): 3.2e-16; 35.9% identity in 259 aa overlap 1 2 5 14 [Reporter]SCO5939 (8A23)_PCR/2117 [Gene]SCO5939/1929 NC_003888 SC7H1.09, probable peptidylprolyl isomerase, len: 157 aa; similar to many eg. Streptomyces chrysomallus TR:P77949 (EMBL:U64692) cyclophilin sccypb (EC 5.2.1.8) (peptidylprolyl isomerase) (175 aa), fasta scores; opt: 402 z-score: 552.5 E(): 1.4e-23, 49.6% identity in 123 aa overlap. Contains Pfam match to entry pro_isomerase PF00160, Peptidyl-prolyl cis-trans isomerases, score 77.82 4 4 18 8 [Reporter]SCO1780 (15P1)_PCR/7940 [Gene]SCO1780/7227 NC_003888 SCI51.20c, possible DNA repair protein, len: 572 aa; similar to SW:RECN_ECOLI (EMBL:Y00357), recN, Escherichia coli DNA repair protein (553 aa), fasta scores; opt: 425 z-score: 428.6 E(): 3.6e-17, 33.1% identity in 568 aa overlap and to recN homologues. Contains probable coiled-coils from 155 to 189 (35 residues) (Max score: 1.472, probability 0.91) and from 336 to 376 (41 residues) (Max score: 1.517, probability 0.96). Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 2 5 13 [Reporter]SCO6448 (9M19)_PCR/2116 [Gene]SCO6448/1928 NC_003888 SC9B5.15, AraC-like transcriptional regulator, len: 313aa; similar to many members of the AraC family of transcriptional regulators eg. SW:MMSR_PSEAE mmsAB operon regulatory protein (307 aa) fasta scores; opt: 171, z-score: 257.7, E(): 4.6e-07, (34.6% identity in 78 aa overlap). Contains PS00041 Bacterial regulatory proteins, araC family signature and Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. 4 4 18 7 [Reporter]SCO0822 (16L1)_PCR/7939 [Gene]SCO0822/7226 NC_003888 SCF43A.12c, possible DNA-binding protein, len: 226 aa; unknown function, weakly similar to SW:YHEO_HAEIN (EMBL:U32739) Haemophilus influenzae hypothetical protein (221 aa), fasta scores; opt: 294 z-score: 349.4 E(): 4e-12, 25.9% identity in 216 aa overlap and SW:YHEO_ECOLI (EMBL:U18997) Escherichia coli hypothetical protein (240 aa) (24.7% identity in 223 aa overlap). C-terminal half shows similarity to TR:Q9Z594 (EMBL:AL035478) S.coelicolor possible DNA-binding protein (122 aa) (38.8% identity in 80 aa overlap). Contains probable helix-turn-helix motif at aa 193-214 (Score 1406, +3.98 SD) 1 2 5 12 [Reporter]SCO1965 (10I19)_PCR/2115 [Gene]SCO1965/1927 NC_003888 SCC54.25c, possible export associated protein, len: 192aa; similar to many associated with plasmid borne resistance export systems egs. TR:Q52353 (EMBL:L38824) from the IncHI2 plasmid R478 of Serratia marcescens (193 aa) fasta scores; opt: 497, z-score: 593.2, E(): 9.8e-26, (42.2% identity in 192 aa overlap) and SW:TERD_ALCSP terD, tellurium resistance protein from the pMER610 plasmid of Alcaligenes sp. (192 aa) fasta scores; opt: 357, z-score: 429.6, E(): 1.3e-16, (37.2% identity in 188 aa overlap). 4 4 18 5 [Reporter]SCO1817 (18D1)_PCR/7937 [Gene]SCO1817/7225 NC_003888 SCI8.02, unknown, len: 464 aa. Similar to many proteins of undefined function e.g. Mycobacterium tuberculosis TR: P71898 (EMBL; Z79702) hypothetical 48.7 KD protein MTCY3G12.20 (457 aa), fasta scores opt: 1485 z-score: 1670.5 E():0 53.5% identity in 454 aa overlap. Contains a Pfam match to entry PF01523 PmbA_TldD, Putative modulator of DNA gyrase. 1 2 5 11 [Reporter]SCO4588 (11E19)_PCR/2114 [Gene]SCO4588/1926 NC_003888 SCD20.06, unknown, len: 508 aa 4 4 18 4 [Reporter]SCO1661 (18P21)_PCR/7936 [Gene]SCO1661/7224 NC_003888 SCI52.03, probable glycerol-3-phosphate dehydrogenase, len: 538 aa; similar to SW:GLPD_BACSU (EMBL:M34393) Bacillus subtilis aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) GlpD, 555 aa; fasta scores: opt: 751 Z-score: 835.1 bits: 164.3 E(): 6.1e-39; 30.830% identity in 506 aa overlap. Contains Pfam match to entry PF01224 FAD_Gly3P_dh, FAD-dependent glycerol-3-phosphate dehydrogenase 1 2 5 10 [Reporter]SCO4291 (12A19)_PCR/2113 [Gene]SCO4291/1925 NC_003888 SCD95A.24, possible secreted protein, len: 233 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence. Contains also a conserved repeat: 4x TP 4 4 18 3 [Reporter]SCO1673 (19L21)_PCR/7935 [Gene]SCO1673/7223 NC_003888 SCI52.15, hypothetical protein, len: 62 aa; low similarity to TR:Q9CC44 (EMBL:AL583921) Mycobacterium leprae hypothetical protein ML1294, 84 aa; fasta scores: opt: 139 Z-score: 215.3 bits: 43.8 E(): 0.0002; 44.000% identity in 50 aa overlap 1 2 5 9 [Reporter]SCO3360 (13M15)_PCR/2112 [Gene]SCO3360/1924 NC_003888 SCE94.11c, unknown, len: 277aa; previously sequenced therefore identical to TR:E1358529 (EMBL:AJ131213) hypothetical protein from Streptomyces coelicolor (277 aa) fasta scores; opt: 1897, z-score: 2249.5, E(): 0, (99.6% identity in 277 aa overlap). 4 4 18 2 [Reporter]SCO5086 (20D21)_PCR/7934 [Gene]SCO5086/7222 NC_003888 SCBAC28G1.12c, actIII, ketoacyl reductase, len: 261 aa; identical to previously sequenced SW:ACT3_STRCO (EMBL:M19536) Streptomyces coelicolor putative ketoacyl reductase ActIII, 261 aa. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 3 3 23 15 [Reporter]SCO1996 (8O14)_PCR/5537 [Gene]SCO1996/5049 NC_003888 SC7H2.10c, coaE, dephospho-CoA kinase, len: 200 aa; similar to many e.g. SW:YTAG_BACSU hypothetical protein from Bacillus subtilis (197 aa) fasta scores; opt: 508, z-score: 575.3, E(): 1e-24, (42.2% identity in 192 aa overlap). Also similar to the N-terminal half of SW:Y14N_MYCTU hypothetical protein from Mycobacterium tuberculosis (407 aa) fasta scores; opt: 672, z-score: 751.8, E(): 0, (54.3% identity in 199 aa overlap). Similar to SW:COAE_ECOLI (EMBL:AE000119) Escherichia coli dephospho-CoA kinase (EC 2.7.1.24) CoaE or B0103, 206 aa; fasta scores: opt: 444 Z-score: 491.1 E(): 1e-19; 44.509% identity in 173 aa overlap. Contains Pfam match to entry PF01121 UPF0038, Uncharacterized protein family UPF0038 and Prosite match to PS01294 Uncharacterized protein family UPF0038 signature 1 2 5 8 [Reporter]SCO0946 (14I15)_PCR/2111 [Gene]SCO0946/1923 NC_003888 SCM11.01c, hypothetical protein, len: >587 aa; similar to various hypothetical proteins, e.g. TR:Q9ZBJ0 (EMBL:AL035161) Streptomyces coelicolor conserved hypothetical protein SC9C7.20, 860 aa; fasta scores: opt: 588 z-score: 654.5 E(): 4.4e-29; 30.4% identity in 542 aa overlap,SCM10.35c, possible regulatory protein, (partial CDS), len: >141 aa; similar to TR:Q9ZBT2 (EMBL:AL034446)Streptomyces coelicolor putative regulatory protein SC1A9.20, 135 aa; fasta scores: opt: 286 z-score: 366.0 E(): 5.3e-13; 42.4% identity in 132 aa overlap 4 4 17 22 [Reporter]SCO1019 (1D17)_PCR/7932 [Gene]SCO1019/7221 NC_003888 2SCG2.33, possible integral membrane protein, len: 411 aa; similar to TR:P74597 (EMBL:D90916) Synechocystis sp. hypothetical 43.6 kDa protein SLR1566, 395 aa; fasta scores: opt: 1119 z-score: 1282.3 E(): 0; 46.3% identity in 402 aa overlap. Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferase and a cleavable N-terminal signal sequence 3 3 23 14 [Reporter]SCO5850 (9K14)_PCR/5536 [Gene]SCO5850/5048 NC_003888 SC9B10.17, unknown, len: 330 aa; similar to TR:Q97U07 (EMBL:AE006910) hypothetical protein from Sulfolobus solfataricus (298 aa) fasta scores; opt: 370, Z-score: 414.6, E(): 1.8e-15, 31.596% identity (34.520% ungapped) in 307 aa overlap. 1 2 5 7 [Reporter]SCO0709 (15E15)_PCR/2110 [Gene]SCO0709/1922 NC_003888 SCF42.19c, possible branched-chain amino acid transport ATP-binding protein, len: 266 aa. Similar to many including: Pseudomonas aeruginosa SW:BRAF_PSEAE(EMBL:D90223) high-affinity branched-chain amino acid transport ATP-binding protein, BraF (255 aa), fasta scores opt: 689 z-score: 786.7 E():0 41.3% identity in 252 aa overlap and Deinococcus radiodurans TR:AAF11669(EMBL:AE002047) branched-chain amino acid ABC transporter, ATP-binding protein (259 aa), fasta scores opt: 749 z-score: 854.2 E():0 46.9% identity in 256 aa overlap. Also similar to Streptomyces coelicolor TR:CAB52067(EMBL:AL109732) putative branched chain amino acid transport ATP-binding protein, SC7H2.25 (309 aa), fasta scores opt: 638 z-score: 728.0 E():0 42.4% identity in 269 aa overlap and to the adjoining CDS on this cosmid, SCF42.20c (263 aa), fasta scores opt: 364 z-score: 308.0 E(): 9.7e-12 35.8% identity in 271 aa overlap. Contains Prosite hits to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Also contains a Pfam match to entry PF00005 ABC_tran, ABC transporter. 4 4 17 21 [Reporter]SCO7590 (2P13)_PCR/7931 [Gene]SCO7590/7220 NC_003888 SC7H9.02c, katA2, catalase, len: 487 aa; similar to SW:CATA_MICLU (PIR:S27264) Micrococcus luteus catalase (EC 1.11.1.6) KatA, 497 aa; fasta scores: opt: 1791 z-score: 2040.9 E(): 0; 54.8% identity in 484 aa overlap and to SW:CATA_STRCO (EMBL:AL035478) Streptomyces coelicolor catalase KatA or SC2G5.25c, 487 aa; fasta scores: opt: 1699 z-score: 1936.3 E(): 0; 53.8% identity in 483 aa overlap. Contains Pfam match to entry PF00199 catalase, Catalase and matches to Prosite entries PS00438 Catalase proximal active site signature and PS00437 Catalase proximal heme-ligand signature 3 3 23 13 [Reporter]SCO1936 (10G14)_PCR/5535 [Gene]SCO1936/5047 NC_003888 SCC22.18, tal2, probable transaldolase, len: 372aa; similar to many both prokaryote and eukaryote egs. TR:O88018 (EMBL:AL031107) tal, transaldolase from Streptomyces coelicolor (381 aa) fasta scores; opt: 1699, z-score: 1924.8, E(): 0, (72.0% identity in 361 aa overlap) and TR:O04894 (EMBL:U95923) transaldolase from Solanum tuberosum (potato) (438 aa) fasta scores; opt: 978, z-score: 1109.7, E(): 0, (48.0% identity in 358 aa overlap). Contains Pfam match to entry PF00923 Transaldolase, Transaldolase and Prosite match to PS01054 Transaldolase signature 1. 1 2 5 6 [Reporter]SCO3133 (16A15)_PCR/2109 [Gene]SCO3133/1921 NC_003888 SCE66.12, probable MarR-family regulator, len: 165 aa; similar to many e.g. SW:P42195 (PECS_ERWCH) regulatory protein PecS from Erwinia chrysanthemi (166 aa) fasta scores; opt: 412, z-score: 499.8, E(): 2.3e-20, 42.7% identity in 150 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family. 3 3 23 12 [Reporter]SCO4100 (11C14)_PCR/5534 [Gene]SCO4100/5046 NC_003888 SCD17.04, unknown, len: 130 aa. 1 2 5 5 [Reporter]SCO1818 (17M11)_PCR/2108 [Gene]SCO1818/1920 NC_003888 SCI8.03, tyrS, tyrosyl-tRNA synthetase (EC 6.1.1.1), len: 422 aa. Highly similar to many tyrosyl-tRNA synthetases e.g. Escherichia coli SW:SYY_ECOLI (EMBL: J01719) (423 aa), fasta scores opt: 1317 z-score: 1463.3 E():0 48.6% identity in 416 aa overlap and Mycobacterium tuberculosis SW:SYY_MYCTU (EMBL: Z98268) (424 aa), fasta scores opt: 1657 z-score: 1839.8 E():0 60.7% identity in 422 aa overlap. Contains a Prosite hit to PS00178 Aminoacyl-transfer RNA synthetases class-I signature and a Pfam match to entry PF00579 tRNA-synt_1b, tRNA synthetases class I (W and Y). 3 3 23 11 [Reporter]SCO4153 (12O10)_PCR/5533 [Gene]SCO4153/5045 NC_003888 SCD84.20c, hypothetical protein, len: 127 aa; similar to TR:CAB58283 (EMBL:AL121850) Streptomyces coelicolor conserved hypothetical protein SCC78.02, 123 aa; fasta scores: opt: 265 z-score: 344.9 E(): 9.4e-12; 40.2% identity in 107 aa overlap. Contains Pfam match to entry PF01638 DUF24, Protein of unknown function 3 3 23 10 [Reporter]SCO3116 (13K10)_PCR/5532 [Gene]SCO3116/5044 NC_003888 SCE41.25c, unknown, len: 335 aa 3 3 23 9 [Reporter]SCO0280 (14G10)_PCR/5531 [Gene]SCO0280/5043 NC_003888 SCF85.08c, unknown, len: 233 aa. Weakly similar to many hypotheticals e.g. Haemophilus influenzae SW:YQIE_HAEIN (EMBL; U32723) hypothetical protein HI0398 (217 aa), fasta scores opt: 291 z-score: 349.5 E(): 4.1e-12 31.8% identity in 179 aa overlap. Contains a PS00017 ATP/GTP-binding site motif A (P-loop). 3 3 23 7 [Reporter]SCO0699 (16O6)_PCR/5529 [Gene]SCO0699/5042 NC_003888 SCF42.09c, unknown, len: 97 aa. 3 3 23 6 [Reporter]SCO3835 (17K6)_PCR/5528 [Gene]SCO3835/5041 NC_003888 SCH69.05, possible dehydrogenase, len: 563aa; similar to many eg. SW:PUTA_ECOLI proline dehydrogenase (1320 aa) fasta scores; opt: 256, z-score: 285.1, E(): 1.5e-08, (27.1% identity in 376 aa overlap). 3 3 23 5 [Reporter]SCO0258 (18G6)_PCR/5527 [Gene]SCO0258/5040 NC_003888 SCF20.04, hypothetical protein, len: 269 aa; similar to TR:BAA81254 (EMBL:AP000063) hypothetical protein from Aeropyrum pernix (238 aa) fasta scores; opt: 228, z-score: 274.4, E(): 6.4e-08, (29.7% identity in 232 aa overlap). 4 4 17 20 [Reporter]SCO6797 (3L13)_PCR/7930 [Gene]SCO6797/7219 NC_003888 SC1A2.06, cvnD7, possible ATP/GTP binding protein, len: 184 aa. Highly similar to many Streptomyces coelicolor putative ATP/GTP binding proteins including: TR:CAA19989(EMBL:AL031124) SC1C2.22C (174 aa), fasta scores opt: 685 z-score: 872.1 E(): 0 60.8% identity in 166 aa overlap, TR:O86519(EMBL:AL031124) SC1C2.18C (174 aa), fasta scores opt: 686 z-score: 873.4 E(): 0 59.6% identity in 166 aa overlap and TR:CAB61279(EMBL:AL132991) SCF55.09C (178 aa),fasta scores opt: 605 z-score: 771.3 E():0 50.8% identity in 177 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 4 4 17 19 [Reporter]SCO5479 (4H13)_PCR/7929 [Gene]SCO5479/7218 NC_003888 SC2A11.13, probable oligopeptide ABC transporter ATP-binding protein, len: 365 aa; similar to many e.g. OPPD_BACSU oligopeptide transport ATP-binding protein (336 aa), fasta scores; opt: 1089 z-score: 1413.1 E(): 0, 52.3% identity in 323 aa overlap Also similar to S. coelicolor TR:P72409 (EMBL:U68036) bldKD (353 aa), fasta scores; opt: 1396 z-score: 1731.9 E(): 0, 58.4% identity in 365 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature and Pfam match to entry PF00005 ABC_tran, ABC transporters, score 203.50, E-value 3.3e-57 1 2 5 4 [Reporter]SCO0621 (18I11)_PCR/2107 [Gene]SCO0621/1919 NC_003888 SCF56.05, hypothetical protein, len: 194 aa; similar to various hypothetical proteins, e.g. SW:YC61_SYNY3 (EMBL:D90909) Synechocystis sp. hypothetical 19.1 kD proetin, 179 aa; fasta scores: opt: 349 z-score: 408.6 E(): 2.2e-15; 41.3% identity in 155 aa overlap 4 4 17 18 [Reporter]SCO6377 (5D13)_PCR/7928 [Gene]SCO6377/7217 NC_003888 SC4A2.13c, probable lipoprotein, len: 451 aa; contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 1 2 5 3 [Reporter]SCO1981 (19E11)_PCR/2106 [Gene]SCO1981/1918 NC_003888 SC3C9.16, hypothetical protein, len: 144 aa; similar to C-terminal part of TR:Q9F397 (EMBL:AL450165) Streptomyces coelicolor hypothetical 21.9 kDa protein SC5F1.32c, 205 aa; fasta scores: opt: 395 Z-score: 465.9 bits: 92.6 E(): 2.4e-18; 43.165% identity in 139 aa overlap 4 4 17 17 [Reporter]SCO6516 (6P9)_PCR/7927 [Gene]SCO6516/7216 NC_003888 SC5C7.01c, unknown, partial CDS, len: >45 aa,SC1E6.25c, unknown, partial CDS, len: > 123 aa; continuation of SC5C7.01c 1 2 5 2 [Reporter]SCO0619 (20M7)_PCR/2105 [Gene]SCO0619/1917 NC_003888 SCF56.03, possible membrane protein, len: 247 aa; similar to various hypothetical proteins, e.g. TR:CAB61305 (EMBL:AL132991) Streptomyces coelicolor hypothetical 25.1 kD protein SCF55.35, 254 aa; fasta scores: opt: 278 z-score: 284.2 E(): 1.9e-08; 31.5% identity in 251 aa overlap 4 4 17 16 [Reporter]SCO6777 (7L9)_PCR/7926 [Gene]SCO6777/7215 NC_003888 SC6A5.26c, possible beta-lactamase, len: 310 aa; similar to SW:BLA1_XANMA L1 metallo-beta-lactamase from Xanthomonas maltophilia (290 aa) fasta scores; opt: 161, z-score: 198.8, E(): 0.0009, (25.5% identity in 247 aa overlap). 1 2 4 22 [Reporter]SCO4191 (1M3)_PCR/2103 [Gene]SCO4191/1916 NC_003888 2SCD46.05, possible hydrolase, len: 273aa; weakly similar to many proposed phosphatases eg. TR:CAB84916 (EMBL:AL162756) putative phosphatase from Neisseria meningitidis (235 aa) fasta scores; opt: 195, z-score: 238.2, E(): 8.8e-06, 29.5% identity in 237 aa overlap. Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase. Note possible alternative downstream translational start codons. 4 4 17 15 [Reporter]SCO5315 (8H9)_PCR/7925 [Gene]SCO5315/7214 NC_003888 SC6G9.18, polyketide cyclase, len: 159 aa; whiE locus polyketide cyclase previously sequenced from S. coelicolor as SW:CYPC_STRCO (EMBL:X55942) 1 2 4 21 [Reporter]SCO1246 (2I3)_PCR/2102 [Gene]SCO1246/1915 NC_003888 2SCG1.21, bioD, dethiobiotin synthetase, len: 238 aa; similar to SW:BIOD_MYCBO (EMBL:AF041819) Mycobacterium bovis dethiobiotin synthetase (EC 6.3.3.3) BioD, 226 aa; fasta scores: opt: 521 z-score: 550.4 E(): 3.4e-23; 45.3% identity in 236 aa overlap 4 4 17 14 [Reporter]SCO5496 (9D9)_PCR/7924 [Gene]SCO5496/7213 NC_003888 SC8D9.08, small hydrophobic membrane protein, len: 123 aa; no significant database similarities. Contains possible hydrophobic membrane spanning regions 1 2 4 20 [Reporter]SCO4926 (3E3)_PCR/2101 [Gene]SCO4926/1914 NC_003888 SCK13.18c, pccB, propionyl-CoA carboxylase complex B subunit, len: 530 aa; identical to previously sequenced TR:Q9X4K7 (EMBL:AF113605) Streptomyces coelicolor propionyl-CoA carboxylase complex B subunit PccB, 530 aa and similar to TR:O86517 (EMBL:AL031124) Streptomyces coelicolor putative carboxyl transferase SC1C2.16, 527 aa; fasta scores: opt: 1958 z-score: 2071.4 E(): 0; 57.4% identity in 526 aa overlap. Contains Pfam match to entry PF01039 Carboxyl_trans, Carboxyl transferase domain 4 4 17 13 [Reporter]SCO2531 (10P5)_PCR/7923 [Gene]SCO2531/7212 NC_003888 SCC117.04, possible beta-glucosidase, len: 448 aa. Highly similar to many including: Microbispora bispora SW:BGLB_MICBI(EMBL:M97265) thermostable beta-glucosidase B (EC 3.2.1.21) (473 aa), fasta scores opt: 1059 z-score: 1221.1 E(): 0 48.7% identity in 454 aa overlap and Paenibacillus polymyxa (Bacillus polymyxa) SW:BGLA_PAEPO(EMBL:M60210) beta-glucosidase A (EC 3.2.1.21) (448 aa), fasta scores opt: 1014 z-score: 1169.7 E():0 40.4% identity in 455 aa overlap. Contains a Prosite hit to PS00653 Glycosyl hydrolases family 1 N-terminal signature and a Pfam match to entry PF00232 Glyco_hydro_1, Glycosyl hydrolase family 1. 3 3 23 4 [Reporter]SCO1368 (19C6)_PCR/5526 [Gene]SCO1368/5039 NC_003888 SC10A9.10c, possible ABC transport system transmembrane protein, len: 379 aa; low similarity to TR:O53617 (EMBL:AL021428) Mycobacterium tuberculosis putative ABC-transporter transmembrane subunit, 349 aa; fasta scores: opt: 175 z-score: 176.6 E(): 0.022; 24.3% identity in 387 aa overlap. Contains possible hydrophobic membrane spanning regions 1 2 4 19 [Reporter]SCO6900 (4A3)_PCR/2100 [Gene]SCO6900/1913 NC_003888 SC1B2.06, unknown, len: 209 aa. Similar to Escherichia coli SW:YIHX_ECOLI (EMBL:L19201) hypothetical 22.7 kd protein (199 aa), fasta scores opt: 181 z-score: 234.7 E(): 1.3e-05 28.0% identity in 189 aa overlap. 4 4 17 12 [Reporter]SCO4371 (11L5)_PCR/7922 [Gene]SCO4371/7211 NC_003888 SCD10.03, conserved hypothetical protein, len: 187 aa; similar to TR:CAB93416 (EMBL:AL357524) Streptomyces coelicolor hypothetical 20.0 kD protein SCD12A.25, 180 aa; fasta scores: opt: 216 z-score: 277.0 E(): 5.9e-08; 32.0% identity in 178 aa overlap. Contains also a 3x degenerate repeat (T/P)TTE 3 3 23 3 [Reporter]SCO1878 (20O2)_PCR/5525 [Gene]SCO1878/5038 NC_003888 SCI39.25c, possible secreted protein, len: 265 aa. Contains possible N-terminal region signal peptide sequence 1 2 4 18 [Reporter]SCO7075 (4M23)_PCR/2099 [Gene]SCO7075/1912 NC_003888 SC3A4.01, two component response regulator protein, len: 230 aa. Highly similar to Pseudomonas syringae (pv. tomato) SW:COPR_PSESM (EMBL:L05176) transcriptional activator of copper resistance operon, CopR (227 aa) fasta scores, opt: 549 z-score: 653.7 E(): 5.9e-29 41.8% identity in 225 aa overlap and Synechococcus sp. (strain PCC 7942) (Anacystis nidulans R2)SW:SPHR_SYNP7(EMBL; D13172) response regulator of alkaline phosphatase, SphR (257 aa) fasta scores, opt: 554 z-score: 658.8 E(): 3.1e-29 40.8% identity in 233 aa overlap. Also similar to Streptomyces coelicolor SCD84.24c (248 aa), fasta scores opt: 767 z-score: 776.3 E():0 51.7% identity in 238 aa overlap. Contains a Pfam match to entry PF00072 response_reg, Response regulator receiver domain and a Pfam match to entry PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal. 4 4 17 11 [Reporter]SCO4799 (12H5)_PCR/7921 [Gene]SCO4799/7210 NC_003888 SCD63A.10c, possible secreted lipase, len: 331 aa; similar to SW:LIP_BACSU (EMBL:M74010) Bacillus subtilis lipase precursor (EC 3.1.1.3) LipA, 212 aa; fasta scores: opt: 188 z-score: 190.5 E(): 0.0038; 26.5% identity in 211 aa overlap and to TR:AAF11628 (EMBL:AE002044) Deinococcus radiodurans lipase, putative, 237 aa; fasta scores: opt: 309 z-score: 302.6 E(): 2.2e-09; 32.0% identity in 200 aa overlap. Contains match to Prosite entry PS00120 Lipases, serine active site and possible N-terminal region signal peptide sequence 3 3 22 22 [Reporter]SCO1086 (1G22)_PCR/5523 [Gene]SCO1086/5037 NC_003888 2SCG4.02c, hypothetical protein, len: 199 aa; similar to TR:P71843 (EMBL:Z80226) Mycobacterium tuberculosis hypothetical 26.7 kD protein MTCY369.34c, 242 aa; fasta scores: opt: 168 z-score: 213.2 E(): 0.00022; 29.7% identity in 111 aa overlap 1 2 4 17 [Reporter]SCO7064 (5I23)_PCR/2098 [Gene]SCO7064/1911 NC_003888 SC4G1.30, possible secreted protein, len: 298 aa. Contains possible N-terminal region signal peptide sequence, possibly cleavable 3 3 22 21 [Reporter]SCO5859 (2C22)_PCR/5522 [Gene]SCO5859/5036 NC_003888 SC9B10.26, hemH, probable ferrochelatase, len: 375 aa; similar to many eg. TR:D1022769 (EMBL:D85417) ferrochelatase (EC 4.99.1.1) from Propionibacterium freudenreichii (352 aa), fasta scores; opt: 491 z-score: 894.8 E(): 0, 42.5% identity in 369 aa overlap 1 2 4 16 [Reporter]SCO6725 (6E23)_PCR/2097 [Gene]SCO6725/1910 NC_003888 SC5F2A.08c, unknown, len: 98 aa; similar to TR:P73587 (EMBL:D90907) hypothetical protein from Synechocystis sp. PCC6803 (89 aa) fasta scores; opt: 99, z-score: 148.0, E(): 0.62, (31.8% identity in 88 aa overlap). 3 3 22 20 [Reporter]SCO6425 (3O18)_PCR/5521 [Gene]SCO6425/5035 NC_003888 SC1A6.14, probable transcriptional regulator, len: 256 aa; weak similarity to e.g. RHAR_ECOLI P09378 Escherichia coli L-rhamnose operon transcriptional regulator RhaR (312 aa), opt: 160 z-score: 207.2 E(): 0.0002, 29.5% identity in 105 aa overlap. Contains PS00041 Bacterial regulatory proteins, araC family signature, Pfam match to entry HTH_2 PF00165, Bacterial regulatory helix-turn-helix proteins, araC family, score 65.61, and probable helix-turn-helix motif at aa 174-195 (Score 1532, +4.40 SD) 3 3 22 19 [Reporter]SCO7609 (4K18)_PCR/5520 [Gene]SCO7609/5034 NC_003888 SC2H2.07c, possible esterase, len: 228 aa; low similarity to TR:Q9K3V2 (EMBL:AL359988) Streptomyces coelicolor putative esterase SCD10.24, 241 aa; fasta scores: opt: 169 z-score: 195.6 E(): 0.0023; 28.0% identity in 236 aa overlap 3 3 22 18 [Reporter]SCO6828 (5G18)_PCR/5519 [Gene]SCO6828/5033 NC_003888 SC4A9.05, possible secreted protein, len: 493 aa. Highly similar to Streptomyces coelicolor putative secreted protein SC1A2.20c (464 aa), fasta scores opt: 1389 z-score: 1246.8 E():0 51.0% identity in 455 aa overlap. Also weakly similar to Streptomyces coelicolor TR:CAB52349(EMBL:AL109747) putative flavin-containing monooxygenase (458 aa), fasta scores opt: 151 z-score: 166.3 E(): 0.075 24.2% identity in 414 aa overlap. Contains a possible N-terminal signal sequence. 3 3 22 16 [Reporter]SCO1412 (7O14)_PCR/5517 [Gene]SCO1412/5032 NC_003888 SC6D7.27c, unknown, len: 277 aa. Highly similar to proteins of unknown function from Bacillus subtilis SW:YCSI_BACSU(EMBL:D38161) hypothetical 28.1 KD protein (257 aa), fasta scores opt: 945 z-score: 1112.7 E():0 52.5% identity in 255 aa overlap and Schizosaccharomyces pombe (Fission yeast) SW:YA01_SCHPO(EMBL:Z49811) hypothetical 33.8 KD protein (301 aa), fasta scores opt: 596 z-score: 703.6 E(): 8.1e-32 40.1% identity in 252 aa overlap. 3 3 22 14 [Reporter]SCO7529 (9G14)_PCR/5515 [Gene]SCO7529/5031 NC_003888 SC8G12.05, unknown, len: 366 aa. Similar to a protein of unknown function from Streptomyces coelicolor TR:Q9Z5B0(EMBL:AL035478) SC2G5.13 (368 aa), fasta scores opt: 688 z-score: 727.2 E():0 44.1% identity in 365 aa overlap. 3 3 22 13 [Reporter]SCO1932 (10C14)_PCR/5514 [Gene]SCO1932/5030 NC_003888 SCC22.14c, unknown, len: 368aa; similar to TR:O07751 (EMBL:Z97193) hypothetical protein from Mycobacterium tuberculosis (378 aa) fasta scores; opt: 772, z-score: 899.4, E(): 0, (40.3% identity in 372 aa overlap). 4 4 17 10 [Reporter]SCO3063 (13D5)_PCR/7920 [Gene]SCO3063/7209 NC_003888 SCE25.04c, probable two-component system reponse regulator, len: 225 aa; similar to TR:CAB77324 (EMBL:AL160331) Streptomyces coelicolor putative response regulator SCD8A.03, 223 aa; fasta scores: opt: 688 z-score: 781.9 E(): 0; 48.9% identity in 223 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 4 4 17 9 [Reporter]SCO0521 (14P1)_PCR/7919 [Gene]SCO0521/7208 NC_003888 SCF11.01, possible tetR family transcriptional regulator, partial CDS, len: >32 aa. Previously annotated as Streptomyces coelicolor SCF6.17, possible tetR family trasncriptional regulator, len: >193 aa. Similar to several e.g. Streptomyces coelicolor TR:CAB56142 (EMBL; AL117669) putative transcriptional regulator SCF12.15 (188 aa), fasta scores opt:446 z-score: 521.2 E(): 1.2e-21 43.5% identity in 184 aa overlap and Streptomyces glaucescens SW:TCMR_STRGA (EMBL; M80674) tetracenomycin C transcriptional repressor (226 aa), fasta scores opt: 202 z-score: 242.5 E(): 3.8e-06 29.3% identity in 164 aa overlap.,SCF6.17, possible tetR family transcriptional regulator, len: 193 aa. Similar to several e.g. Streptomyces coelicolor TR:CAB56142 (EMBL; AL117669) putative transcriptional regulator SCF12.15 (188 aa), fasta scores opt:446 z-score: 521.2 E(): 1.2e-21 43.5% identity in 184 aa overlap and Streptomyces glaucescens SW:TCMR_STRGA (EMBL; M80674) tetracenomycin C transcriptional repressor (226 aa), fasta scores opt: 202 z-score: 242.5 E(): 3.8e-06 29.3% identity in 164 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. The possible helix-turn-helix motif lies between residues 29..50 (+2.70 SD). 1 2 4 15 [Reporter]SCO7436 (7A23)_PCR/2096 [Gene]SCO7436/1909 NC_003888 SC6D11.32c, probable aldehyde dehydrogenase, len: 484 aa. Highly similar to many dehydrogenases including: Pseudomonas putida SW:XYLC_PSEPU(EMBL:U15151) benzaldehyde dehydrogenase [NAD+] (EC 1.2.1.28) (487 aa), fasta scores opt: 1519 z-score: 1634.7 E():0 47.6% identity in 483 aa overlap and Streptomyces coelicolor TR:Q9X8J9(EMBL:AL049841) putative aldehyde dehydrogenase, SCE9.27C (486 aa), fasta scores opt: 1215 z-score: 1308.5 E():0 43.1% identity in 476 aa overlap. Contains a Prosite hit to PS00687 Aldehyde dehydrogenases glutamic acid active site and a Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family. 4 4 17 8 [Reporter]SCO0101 (15L1)_PCR/7918 [Gene]SCO0101/7207 NC_003888 SCJ11.30c, unknown, len: 247 aa; similar to SW:YBFG_HAEIN hypothetical protein from Haemophilus influenzae (228 aa) fasta scores; opt: 374, z-score: 437.3, E(): 5.3e-17, (32.8% identity in 195 aa overlap). Contains TTA leucine codon, possible target for bldA regulation. 1 2 4 14 [Reporter]SCO7601 (8M19)_PCR/2095 [Gene]SCO7601/1908 NC_003888 SC7H9.13c, probable amidase, len: 490 aa; similar to TR:Q9LCX3 (EMBL:AF202447) Thermus aquaticus Glu/Asp-tRNA amidotransferase subunit A, GatA, 471 aa; fasta scores: opt: 614 z-score: 609.3 E(): 2.1e-26; 34.7% identity in 470 aa overlap. Contains Pfam match to entry PF01425 Amidase, Amidase and match to Prosite entry PS00571 Amidases signature 4 4 17 7 [Reporter]SCO0945 (16H1)_PCR/7917 [Gene]SCO0945/7206 NC_003888 SCM10.34c, probable formamidopyrimidine-DNA glycosylase len: 287 aa; similar to TR:P71565 (EMBL:Z79700) Mycobacterium tuberculosis hypothetical 16.5k kD protein MTYC10D7.30c, 158 aa; fasta scores: opt: 608 z-score: 707.4 E(): 5.2e-32; 59.0% identity in 156 aa overlap and to SW:FPG_SYNEN (EMBL:Y10290) Synechococcus elongatus naegeli formamidopyrimidine-DNA glycosylase (EC 3.2.2.23) MutM, 284 aa; fasta scores: opt: 426 z-score: 495.1 E(): 3.4e-20; 33.6% identity in 283 aa overlap. Contains Pfam match to entry PF01149 Fapy_DNA_glyco, Formamidopyrimidine-DNA glycosylase 1 2 4 13 [Reporter]SCO6444 (9I19)_PCR/2094 [Gene]SCO6444/1907 NC_003888 SC9B5.11, probable gamma-glutamyl transferase, len: 647 aa; similar to many gamma-glutamyl transferases eg. TR:Q54353 (EMBL:X79146) LmbA, gamma-glutamyl transferase involved in lincomycin production in Streptomyces lincolnensis 78-11 (601 aa) fasta scores; opt: 871, z-score: 1756.8, E(): 0, (48.1% identity in 619 aa overlap). Also similar to TR:O54174 (EMBL:AL021411) probable transferase (606aa) from Streptomyces coelicolor fasta scores: opt: 953, z-score: 1203.1, E(): 0, (53.7% identity in 635 aa overlap). Contains Pfam match to entry PF01019 G_glu_transpept, Gamma-glutamyltranspeptidase 4 4 17 6 [Reporter]SCO3415 (17D1)_PCR/7916 [Gene]SCO3415/7205 NC_003888 SCE9.22, probable esterase, len: 266 aa; similar to many e.g. SW:BAH_STRHY (EMBL:M64783), bah, Streptomyces hygroscopicus acetyl-hydrolase in bialaphos biosynthetic gene cluster (299 aa), fasta scores; opt: 637 z-score: 678.5 E(): 1.8e-30, 45.3% identity in 236 aa overlap. Contains PS01173 Lipolytic enzymes 'G-D-X-G' family, putative histidine active site 1 2 4 12 [Reporter]SCO2296 (10E19)_PCR/2093 [Gene]SCO2296/1906 NC_003888 SCC30.04, possible integral membrane protein, len: 204 aa; similar to TR:AAF12504 (EMBL:AE001863) Deinococcus radiodurans hypothetical 32.0 kD protein, 289 aa; fasta scores: opt: 178 z-score: 231.0 E(): 2e-05; 29.8% identity in 161 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 17 5 [Reporter]SCO3357 (17P21)_PCR/7915 [Gene]SCO3357/7204 NC_003888 SCE94.08, unknown, len: 202aa; previously sequenced therefore identical to TR:e1358526 (EMBL:AJ131213) hypothetical protein from Streptomyces coelicolor (202 aa) fasta scores; opt: 1347, z-score: 1386.9, E(): 0, (99.5% identity in 202 aa overlap). Note conflict in first codon, GTG rather than ATG. 1 2 4 11 [Reporter]SCO4584 (11A19)_PCR/2092 [Gene]SCO4584/1905 NC_003888 SCD20.02, possible membrane protein, len: 420 aa. Contains possible hydrophobic membrane spanning region 4 4 17 4 [Reporter]SCO1313 (18L21)_PCR/7914 [Gene]SCO1313/7203 NC_003888 SCBAC36F5.24, possible integral membrane protein, len: 285 aa; similar to TR:Q9RRQ1 (EMBL:AE002073) Deinococcus radiodurans hypothetical 27.6 kDa protein DR2437, 278 aa; fasta scores: opt: 307 Z-score: 316.3 bits: 66.4 E(): 5.2e-10; 35.052% identity in 291 aa overlap. Contains 2x Pfam matches to entry PF00892 DUF6, Integral membrane protein DUF6 and possible hydrophobic membrane spanning regions 1 2 4 10 [Reporter]SCO3297 (12M15)_PCR/2091 [Gene]SCO3297/1904 NC_003888 SCE15.14, putative membrane protein, len: 112 aa; similar to others e.g. SW:YQ09_MYCTU (EMBL:Z80225) Mycobacterium tuberculosis hypothetical protein (110 aa) fasta scores; opt: 597, z-score: 741.6, E(): 0, (73.1% identity in 108 aa overlap) Contains possible hydrophobic membrane spanning regions 4 4 17 3 [Reporter]SCP1.203c (19H21)_PCR/7913 [Gene]SCP1.203c/7202 NC_003888 SCP1.203c, unknown, len: 170aa; 3 3 22 12 [Reporter]SCO4334 (11O10)_PCR/5513 [Gene]SCO4334/5029 NC_003888 SCD12A.17, possible integral membrane protein, len: 485 aa; similar to TR:Q9ZBN6 (EMBL:AL034492) Streptomyces coelciolor hypothetical 50.0 kD protein SC6C5.09, 485 aa; fasta scores: opt: 1532 z-score: 1657.3 E(): 0; 51.1% identity in 456 aa overlap. Contains Pfam match to entry PF00860 xan_ur_permease, Xanthine/uracil permeases family and match to Prosite entry PS00012 Phosphopantetheine attachment site. Contains also possible hydrophobic membrane spanning regions 1 2 4 9 [Reporter]SCO3687 (13I15)_PCR/2090 [Gene]SCO3687/1903 NC_003888 SCH35.37c, unknown, len: 199aa; N-terminal region alanine-rich, C-terminal region glutamic acid-rich. Similar to TR:CAA22040 (EMBL:AL033505) hypothetical protein from Streptomyces coelicolor (239 aa) fasta scores; opt: 205, z-score: 204.5, E(): 0.00046, (36.1% identity in 202 aa overlap). 4 4 17 2 [Reporter]SCO7487 (20P17)_PCR/7912 [Gene]SCO7487/7201 NC_003888 SCBAC17A6.20c, possible integral membrane binding protein dependent transport protein, len: 294aa; similar to many eg. TR:BAB49483 (EMBL:AP002999) permease protein of sugar ABC transporter from Rhizobium loti (296 aa) fasta scores: opt: 608, Z-score: 696.1, 35.570% identity (36.934% ungapped) in 298 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and multiple hydrophobic possible membrane spanning regions. 3 3 22 11 [Reporter]SCO4149 (12K10)_PCR/5512 [Gene]SCO4149/5028 NC_003888 SCD84.16, possible ABC transport system transmembrane protein, len: 509 aa; N-terminal region similar to TR:CAB50958 (EMBL:AL096849) Streptomyces coelicolor putative ABC-transporter transmembrane protein SCI11.32, 239 aa; fasta scores: opt: 142 z-score: 168.1 E(): 0.066; 27.9% identity in 244 aa overlap. Contains possible hydrophobic membrane spanning regions in N-terminal region 1 2 4 8 [Reporter]SCO0181 (14E15)_PCR/2089 [Gene]SCO0181/1902 NC_003888 SCJ1.30c, unknown, len: 328 aa. Similar to many including: Mycobacterium tuberculosis SW:YW23_MYCTU (EMBL; Z74025) hypothetical 33.9 KD protein MTCY39.23C (317 aa) fasta scores opt: 382 z-score: 439.2 E(): 4.2e-17 32.6% identity in 319 aa overlap and Mycobacterium tuberculosis TR:O53472 (EMBL; AL021899) hypothetical 31.5 KD protein (294 aa), fasta scores opt: 382 z-score: 439.2 E(): 4.2e-17 32.6% identity in 319 aa overlap. Contains a Pfam match to entry PF00582 Usp, Universal stress protein family. 4 4 16 22 [Reporter]SCO1205 (1P13)_PCR/7910 [Gene]SCO1205/7200 NC_003888 2SCG58.05, unknown, len: 74 aa 3 3 22 10 [Reporter]SCO2999 (13G10)_PCR/5511 [Gene]SCO2999/5027 NC_003888 SCE33.01c, hypothetical protein (fragment), len: >187 aa; similar to TR:Q9X7B2 (EMBL:AL049913) Mycobacterium leprae hypothetical 177.9 kD protein MCLB1610.10, 1622 aa; fasta scores: opt: 154 z-score: 172.5 E(): 0.038; 29.7% identity in 155 aa overlap,SCE99.06c, conserved hypothetical protein (fragment), len: >1515 aa; similar to TR:AAG06456 (EMBL:AE004731) Pseudomonas aeruginosa conserved hypothetical protein PA3068, 1620 aa; fasta scores: opt: 4170 z-score: 4589.7 E(): 0; 46.3% identity in 1521 aa overlap 1 2 4 7 [Reporter]SCO0892 (15A15)_PCR/2088 [Gene]SCO0892/1901 NC_003888 SCM1.25c, possible integral membrane protein, len: 316 aa. Highly similar to several other putative membrane proteins including: Serratia marcescens SW:TERC_SERMA(EMBL:L38824) tellurium resistance protein TerC (346 aa), fasta scores opt: 381 z-score: 448.5 E(): 1.2e-17 32.3% identity in 325 aa overlap and Streptomyces coelicolor TR:Q9Z503(EMBL:AL035591) putative integral membrane export protein SCC54.23c (333 aa), fasta scores opt: 1690 z-score: 1964.9 E(): 0 79.1% identity in 316 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 3 3 22 8 [Reporter]SCO5158 (15O6)_PCR/5509 [Gene]SCO5158/5026 NC_003888 SCP8.21, unknown, len: 198 aa 1 2 4 6 [Reporter]SCO1022 (16M11)_PCR/2087 [Gene]SCO1022/1900 NC_003888 SCG20A.02, hypothetical protein, len: 275 aa; similar to C-terminal part of TR:Q9XFN9 (EMBL:AC000107) Arabidopsis thaliana F17F8.6, 325 aa; fasta scores: opt: 491 z-score: 580.3 E(): 7.6e-25; 34.1% identity in 305 aa overlap and to TR:Q9HZI4 (EMBL:AE004727) Pseudomonas aeruginosa hypothetical protein PA3022, 268 aa; fasta scores: opt: 688 Z-score: 795.9 E(): 1.1e-36; 45.788% identity in 273 aa overlap 3 3 22 7 [Reporter]SCO3474 (16K6)_PCR/5508 [Gene]SCO3474/5025 NC_003888 SCE65.10c, possible sugar kinase, len: 333 aa; similar to SW:KDGK_BACSU (EMBL:L47838) Bacillus subtilis 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45) KdgK, 324 aa; fasta scores: opt: 500 z-score: 585.4 E(): 3.1e-25; 34.1% identity in 299 aa overlap. Contains Pfam match to entry PF00294 pfkB, pfkB family carbohydrate kinase 3 3 22 6 [Reporter]SCO3553 (17G6)_PCR/5507 [Gene]SCO3553/5024 NC_003888 SCH5.16c, putative small membrane protein, len: 75 aa; similar to TR:O69624 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (68 aa) fasta scores; opt: 129, z-score: 191.2, E(): 0.0024, (40.0% identity in 60 aa overlap). Contains possible hydrophobic membrane spanning regions 3 3 22 5 [Reporter]SCO3584 (18C6)_PCR/5506 [Gene]SCO3584/5023 NC_003888 SCH66.05c, unknown, len: 119 aa; some similarity to TR:O86817 (EMBL:AL031031) very hypothetical protein from Streptomyces coelicolor (88 aa) fasta scores; opt: 96, z-score: 132.6, E(): 4.6, (48.4% identity in 62 aa overlap). 3 3 22 4 [Reporter]SCP1.85 (19O2)_PCR/5505 [Gene]SCP1.85/5022 NC_003888 SCP1.85, possible membrane protein, len: 439aa; contains possible membrane-spanning hydrophobic regions. 3 3 22 3 [Reporter]SCO4038 (20K2)_PCR/5504 [Gene]SCO4038/5021 NC_003888 2SCD60.04c, possible deaminase, len: 143 aa; similar to TR:AAG28837 (EMBL:AF160969) Bifidobacterium longum cytosine deaminase, 143 aa; fasta scores: opt: 553 z-score: 690.3 E(): 6.9e-31; 56.6% identity in 143 aa overlap. Contains Pfam match to entry PF00383 dCMP_cyt_deam, Cytidine and deoxycytidylate deaminase zinc-binding region and match to Prosite entry PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature 3 3 21 22 [Reporter]SCO1145 (1C22)_PCR/5501 [Gene]SCO1145/5020 NC_003888 2SCG38.38, possible marR-family regulator, len: 153 aa; similar to TR:Q9RDN9 (EMBL:AL133423) Streptomyces coelicolor putative marR-family transcriptional regulator SC4A7.26, 151 aa; fasta scores: opt: 200 z-score: 252.2 E(): 1.3e-06; 36.1% identity in 147 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 3 3 21 21 [Reporter]SCO5008 (2O18)_PCR/5500 [Gene]SCO5008/5019 NC_003888 SCK15.10, minD3, possible septum site-determining protein, len: 131 aa; similar to TR:Q9ZF15 (EMBL:AJ005576) Streptomyces griseus septum site-determining protein MinD protein, 396 aa; fasta scores: opt: 180 z-score: 224.4 E(): 5e-05; 37.7% identity in 122 aa overlap and to Streptomyces coelicolor septum site-determining protein minD2, 131 aa; fasta scores: opt: 177 z-score: 189.4 E(): 5.4e-05; 36.4% identity in 107 aa overlap. Contains possible hydrophobic membrane spanning region 3 3 21 20 [Reporter]SCO6421 (3K18)_PCR/5499 [Gene]SCO6421/5018 NC_003888 SC1A6.10, probable two-component system sensor kinase, len: 382 aa; N-terminus (~1-150 aa) contains six potential transmembrane domains, C-terminus is similar to e.g. SW:UHPB_ECOLI (P09835) Escherichia coli sensor protein UhpB (500 aa), fasta scores; opt: 151 z-score: 290.1 E(): 4.9e-09, 28.3% identity in 314 aa overlap and to many Streptomyces coelicolor putative two-component system sensor kinases, e.g. TR:Q9S2S0 (EMBL:AL09687) putative two-component system sensor kinase SC5F7.35c, 416 aa; fasta scores: opt: 525 Z-score: 546.5 E(): 8.3e-23; 40.489% identity in 368 aa overlap. Contains possible hydrophobic membrane spanning regions 3 3 21 19 [Reporter]SCO5609 (4G18)_PCR/5498 [Gene]SCO5609/5017 NC_003888 SC2E1.26c, unknown prophage gene, len: 65 aa; very hydrophobic. Contains possible hydrophobic membrane spanning region 3 3 21 18 [Reporter]SCO6824 (5C18)_PCR/5497 [Gene]SCO6824/5016 NC_003888 SC4A9.01c, possible phosphonopyruvate decarboxylase, partial CDS, len: >32 aa. Highly similar to Streptomyces coelicolor TR:Q9XBE9(EMBL:AL078635) putative phosphonopyruvate decarboxylase, CZA382.02 (371 aa), fasta scores opt:95 z-score: 160.0 E():0.17 51.7% identity in 29 aa overlap. Overlaps and extends into CDS SC1A2.33c on the adjoining cosmid.,SC1A2.33c, possible phosphonopyruvate decarboxylase, partial CDS, len: >366 aa. Highly similar to several including: Streptomyces wedmorensis TR:Q56190(EMBL:AB016934) phosphonopyruvate decarboxylase (384 aa), fasta scores opt: 391 z-score: 431.7 E(): 1.2e-16 37.8% identity in 347 aa overlap and Streptomyces coelicolor TR:Q9XBE9(EMBL:AL078635) putative phosphonopyruvate decarboxylase, CZA382.02 (371 aa), fasta scores opt: 1134 z-score: 1240.1 E(): 0 49.2% identity in 366 aa overlap. Contains a Prosite hit to PS00187 Thiamine pyrophosphate enzymes signature. 3 3 21 17 [Reporter]SCO6752 (6O14)_PCR/5496 [Gene]SCO6752/5015 NC_003888 SC6A5.01, partial CDS, possible integral membrane transferase, len: >490aa; similar to TR:O67379 (EMBL:AE000737) glucose-1-phosphate thymidylyltransferase from Aquifex aeolicus (428 aa) fasta scores; opt: 215, z-score: 245.5, E(): 2.2e-06, (26.2% identity in 210 aa overlap). Contains Prosite match to PS00379 CDP-alcohol phosphatidyltransferases signature and possible membrane spanning hydrophobic regions.,SC5F2A.35, partial CDS, possible transferase, len: >148aa; C-terminal region lies on cosmid 6A5 (EMBL:AL049485). Similar over a defined region to many proposed/hypothetical proteins eg. TR:O67379 (EMBL:AE000737) proposed glucose-1-phosphate thymidylyltransferase from Aquifex aeolicus (428 aa) fasta scores; opt: 215, z-score: 239.5, E(): 5e-06, (26.2% identity in 210 aa overlap). Contains Prosite match to PS00379 CDP-alcohol phosphatidyltransferases signature. Also contains possible membrane spanning hydrophobic regions. 3 3 21 16 [Reporter]SCO1416 (7K14)_PCR/5495 [Gene]SCO1416/5014 NC_003888 SC6D7.23c, possible membrane protein, len: 582 aa. Contains a possible membrane spanning hydrophobic domain close to th N-terminus. 3 3 21 15 [Reporter]SCO5222 (8G14)_PCR/5494 [Gene]SCO5222/5013 NC_003888 SC7E4.19, possible lyase, len: 361 aa; similar to SW:PTLS_STRSQ (EMBL:U05213) Streptomyces sp. pentalenene synthase (EC 4.6.1.5), 336 aa; fasta scores: opt: 254 z-score: 317.5 E(): 3.3e-10; 23.8% identity in 311 aa overlap. Contains match to Prosite entry PS00904 Protein prenyltransferases alpha subunit repeat signature and a TTA leucine codon, possible target for bldA regulation 3 3 21 14 [Reporter]SCO5421 (9C14)_PCR/5493 [Gene]SCO5421/5012 NC_003888 SC8F4.25, possible integral membrane protein, len: 185 aa. Contains possible hydrophobic membrane spanning regions 3 3 21 13 [Reporter]SCO2574 (10O10)_PCR/5492 [Gene]SCO2574/5011 NC_003888 SCC123.12c, doubtful CDS, len: 77 aa. 3 3 21 12 [Reporter]SCO4330 (11K10)_PCR/5491 [Gene]SCO4330/5010 NC_003888 SCD12A.13, hypothetical protein, len: 308 aa; similar to TR:O33058 (EMBL:Z99494) Mycobacterium leprae hypothetical 28.2 kD protein MLCB57.28, 269 aa; fasta scores: opt: 432 z-score: 470.7 E(): 9.4e-19; 43.3% identity in 270 aa overlap 3 3 21 11 [Reporter]SCO4170 (12G10)_PCR/5490 [Gene]SCO4170/5009 NC_003888 SCD66.07, possible membrane protein, len: 301 aa. Contains possible hydrophobic membrane spanning region and high content in alanine amino acid residues 3 3 21 10 [Reporter]SCO3248 (13C10)_PCR/5489 [Gene]SCO3248/5008 NC_003888 SCE29.17c, fabF3, probable 3-oxoacyl-[acyl carrier protein] synthase II, len: 406 aa; similar to many e.g. TR:O54149 (EMBL:AL021409) 3-oxoacyl acyl carrier protein synthase II from the Red cluster of Streptomyces coelicolor (407 aa) fasta scores; opt: 1217, z-score: 1314.2, E(): 0, (50.1% identity in 407 aa overlap) and SW:FABF_ECOLI 3-oxoacyl-[acyl carrier protein] synthase II FabF, from Escherichia coli (412 aa) fasta scores; opt: 843, z-score: 912.2, E(): 0, (35.4% identity in 404 aa overlap). Contains Pfam match to entry PF00109 ketoacyl-synt, Beta-ketoacyl synthase, score 92.60, E-value 7.7e-24 and Prosite match PS00606 Beta-ketoacyl synthases active site. 3 3 21 9 [Reporter]SCO0756 (14O6)_PCR/5488 [Gene]SCO0756/5007 NC_003888 SCF81.15, possible ABC transporter, len: 940 aa; similar to TR:P74829 (EMBL:U51197) Sphingomonas sp. S88 hypothetical 79.4 KD protein AtrB, 728 aa; fasta scores: opt: 803 z-score: 870.4 E(): 0; 32.7% identity in 562 aa overlap and to SW:CYAB_BORPE (EMBL:X14199) Bordetella pertussis cyclolysin secretion ATP-binding protein CyaB, 712 aa; fasta scores: opt: 785 z-score: 851.0 E(): 0; 32.2% identity in 631 aa overlap. Contains two matches to Pfam entries PF00664 ABC_membrane, ABC transporter transmembrane region and PF00005 ABC_tran, ABC transporter and two to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 3 21 8 [Reporter]SCO0831 (15K6)_PCR/5487 [Gene]SCO0831/5006 NC_003888 SCF43A.21c, hypothetical protein, len: 306 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Similar to the adjacent protein SCF43A.22c, hypothetical protein (321 aa), fasta scores; opt: 674 z-score: 697.3 E(): 1.1e-33, 41.3% identity in 300 aa overlap 3 3 21 7 [Reporter]SCO5159 (16G6)_PCR/5486 [Gene]SCO5159/5005 NC_003888 SCP8.22c, possible integral membrane transport protein, len: 407 aa; similar to TR:CAB76344 (EMBL:AL158061) Streptomyces coelicolor putative multidrug-efflux transporter protein SC6D11.21, 577 aa; fasta scores: opt: 1286 z-score: 1436.2 E(): 0; 51.1% identity in 393 aa overlap and to SW:BMR1_BACSU (EMBL:M33768) Bacillus subtilis multidrug resistance protein 1 Bmr1, 389 aa; fasta scores: opt: 262 z-score: 298.0 E(): 3.9e-09; 22.9% identity in 375 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and possible hydrophobic membrane spanning regions 3 3 21 6 [Reporter]SCO0036 (17C6)_PCR/5485 [Gene]SCO0036/5004 NC_003888 SCJ4.17c, unknown, len: 79 aa; weak similarity to TR:Q48505 (EMBL:M63292;) beta-lactamase (fragment) from Lactococcus lactis subsp. lactis (87 aa) fasta scores; opt: 91, z-score: 133.7, E(): 4.4, (31.8% identity in 66 aa overlap). 3 3 21 5 [Reporter]SCO1546 (18O2)_PCR/5484 [Gene]SCO1546/5003 NC_003888 SCL11.02c, probable aminotransferase, len: 273 aa; similar to SW:DAAA_BACSU (EMBL:)Y14082 Bacillus subtilis D-alanine aminotransferase (EC 2.6.1.21) Dat, 282 aa; fasta scores: opt: 347 z-score: 415.1 E(): 1.1e-15; 28.5% identity in 274 aa overlap. Contains Pfam match to entry PF01063 aminotran_4, Aminotransferase class IV and match to Prosite entry PS00770 Aminotransferases class-IV signature 3 3 21 4 [Reporter]SCP1.176 (19K2)_PCR/5483 [Gene]SCP1.176/5002 NC_003888 SCP1.176, possible large membrane protein, len: 1203aa; N-terminal region contains three possible membrane-spanning hydrophobic regions. Remainder similar to TR:Q9XAP0 (EMBL:AL078618) hypothetical protein from Streptomyces coelicolor (726 aa) fasta scores; opt: 457, z-score: 394.3, E(): 1.7e-14, 26.8% identity in 898 aa overlap. 3 3 21 3 [Reporter]SCO5354 (20G2)_PCR/5482 [Gene]SCO5354/5001 NC_003888 SCBAC5H2.23, thrA, homoserine dehydrogenase, len: 429 aa; highly similar to SW:DHOM_CORGL (EMBL:Y00546) Corynebacterium glutamicum homoserine dehydrogenase (EC 1.1.1.3) Hom or ThrA, 445 aa; fasta scores: opt: 1592 z-score: 1742.3 E(): 0; 57.7% identity in 423 aa overlap. Contains Pfam matches to entries PF00742 Homoserine_dh, Homoserine dehydrogenase and PF01842 ACT, ACT domain and match to Prosite entry PS01042 Homoserine dehydrogenase signature. Note: Can participate in the biosynthesis of Methionine, Lysine and Threonine (plus following steps to Valine, Leucine and Isoleucine) 3 3 21 2 [Reporter]SCO5979 (20O22)_PCR/5481 [Gene]SCO5979/5000 NC_003888 SCBAC16H6.14, possible enoyl-CoA hydratase, len: 275 aa: similar to many e.g. SW:P77467 (PAAG_ECOLI) probable enoyl-CoA hydratase from Escherichia coli (262 aa) fasta scores; opt: 488, Z-score: 554.6, 36.434% identity (37.450% ungapped) in 258 aa overlap and TR:Q9AJT1 (EMBL:AJ278289) hypothetical protein from Thauera aromatica (272 aa) fasta scores; opt: 780, Z-score: 880.5, 44.318% identity (44.656% ungapped) in 264 aa overlap. Contains Pfam match to entry PF00378 ECH, Enoyl-CoA hydratase/isomerase family and Prosite match to PS00166 Enoyl-CoA hydratase/isomerase signature. 3 4 19 10 [Reporter]SCO3263 (13K5)_PCR/7460 [Gene]SCO3263/6799 NC_003888 SCE39.13c, unknown, len: 99 aa; similar to TR:CAC44711 (EMBL:AL596251) Streptomyces coelicolor conserved hypothetical protein SCBAC25E3.24, 101 aa; fasta scores: opt: 441 Z-score: 540.6 E(): 1.8e-22; 67.742% identity in 93 aa overlap. Contains possible coiled-coil region 3 4 19 9 [Reporter]SCO1184 (14G5)_PCR/7459 [Gene]SCO1184/6798 NC_003888 SCG11A.15, hypothetical protein, len: 1320 aa; similar to various hypothetical proteins, e.g. TR:CAB46976 (EMBL:AL096825) Streptomyces coelicolor hypothetical 122.6 KD protein SC6G3.04, 1171 aa; fasta scores: opt: 1532 z-score: 1249.6 E(): 0; 50.6% identity in 1315 aa overlap and to SW:YEHP_ECOLI (EMBL:U00007) Escherichia coli hypothetical 42.1 KD protein in MolR-BglX intergenic region YehP, 378 aa; fasta scores: opt: 660 z-score: 547.5 E(): 4e-23; 37.5% identity in 379 aa overlap 3 4 19 8 [Reporter]SCO3817 (15C5)_PCR/7458 [Gene]SCO3817/6797 NC_003888 SCGD3.18c, bkdA1, probable branched-chain alpha keto acid dehydrogenase E1 alpha subunit, len: 417 aa; similar to E1 alpha subunits from pyruvate dehydrogenase complexes and branched-chain alpha keto acid dehydrogenases e.g. TR:Q53610 (EMBL:U26308) Streptomyces avermitilis branched-chain alpha-keto acid dehydrogenase E1-alpha subunit (406 aa), fasta scores; opt: 2177 z-score: 2466.3 E(): 0, 80.9% identity in 409 aa overlap and SW:ODPA_BACST (EMBL:X53560), PdhA, Bacillus stearothermophilus pyruvate dehydrogenase complex E1 alpha subunit (368 aa) (39.7% identity in 325 aa overlap). Similar to SCGD3.32c, probable branched-chain alpha keto acid E1 alpha subunit, partial CDS (179 aa) (38.8% identity in 165 aa overlap), in a duplication of this region upstream. Contains Pfam match to entry PF00676 E1_dehydrog, Dehydrogenase E1 component 3 4 19 7 [Reporter]SCO0906 (16O1)_PCR/7457 [Gene]SCO0906/6796 NC_003888 SCM1.39c, unknown, len: 165 aa. Weakly similar to several proteins of undefined function e.g. Mycobacterium tuberculosis TR:O53240(EMBL:AL021287) hypothetical 18.2 KD protein (163 aa), fasta scores opt: 148 z-score: 178.3 E(): 0.014 30.9% identity in 139 aa overlap. 3 4 19 6 [Reporter]SCO3227 (17K1)_PCR/7456 [Gene]SCO3227/6795 NC_003888 SCE8.20c, partial CDS, possible aminotransferase, len: 431 aa; similar to many of undefined function e.g. TR:O52815 (EMBL:AJ223998) a protein similar to aminotransferase, from a cluster of genes involved in the biosynthesis of a vancomycin group antibiotic in Amycolatopsis orientalis (Actinomycete) (438 aa) fasta scores; opt: 953, z-score: 1093.1, E(): 0, (48.4% identity in 426 aa overlap). Also similar to TR:Q64602 (EMBL:Z50144) kynurenine/alpha-aminoadipate aminotransferase from rat (Rattus norvegicus) kidney (425 aa) fasta scores; opt: 293, z-score: 338.4, E(): 1.5e-11, (25.8% identity in 419 aa overlap).,SCE63.06, partial CDS, possible aminotransferase, len: >45 aa; similar to the N-terminal region of TR:O52815 (EMBL:AJ223998) a protein similar to aminotransferase from a cluster of genes nvolved in the biosynthesis of a vancomycin group antibiotic in Amycolatopsis orientalis (Actinomycete) (438 aa) fasta scores; opt: 87, z-score: 147.0, E(): 0.69, (45.2% identity in 31 aa overlap). The remainder of this CDS lies on the neighbouring Streptomyces coelicolor cosmid E8. 3 4 19 5 [Reporter]SCO3350 (18G1)_PCR/7455 [Gene]SCO3350/6794 NC_003888 SCE94.01c, partial CDS unknown, alanine-rich protein, len: >503aa;,SCE7.17c, partial CDS, proline-rich protein, len: >129aa; 3 4 19 4 [Reporter]SCO7523 (19C1)_PCR/7454 [Gene]SCO7523/6793 NC_003888 SCBAC25F8.15, possible chaperone, len: 837 aa; highly similar to SW:CLPC_BACSU (EMBL:D26185) Bacillus subtilis negative regulator of genetic competence ClpC or MecB, 810 aa; fasta scores: opt: 2262 Z-score: 2131.8 bits: 405.4 E(): 3.9e-111; 50.807% identity in 805 aa overlap and to TR:O69936 (EMBL:AL023861) Streptomyces coelicolor Clp protease ATP binding subunit CplA or SC3C8.27c, 842 aa; fasta scores: opt: 2590 Z-score: 2440.2 bits: 462.5 E(): 2.6e-128; 55.952% identity in 840 aa overlap. Contains 2x Pfam matches to entry PF02861 Clp_N, Clp amino terminal domain, match to entry PF00004 AAA, ATPase family associated with various cellular activities (AAA) and matches to Prosite entries 2x PS00017 ATP/GTP-binding site motif A (P-loop,), PS00870 Chaperonins clpA/B signature 1 and PS00871 Chaperonins clpA/B signature 2 3 4 19 3 [Reporter]SCO6092 (19O21)_PCR/7453 [Gene]SCO6092/6792 NC_003888 SCBAC1A6.16, conserved hypothetical protein, len: 182 aa; similar to TR:O06552 (EMBL:Z95584) Mycobacterium tuberculosis hypothetical 23.0 kDa protein MTCI65.21c, 213 aa; fasta scores: opt: 229 z-score: 293.1 E(): 9.5e-09; 31.8% identity in 179 aa overlap 3 4 19 2 [Reporter]SCO5113 (20G21)_PCR/7452 [Gene]SCO5113/6791 NC_003888 SCBAC31E11.09, bldKB, possible ABC transport system lipoprotein, len: 600 aa; almost identical to TR:P72407 (EMBL:U68036) Streptomyces coelicolor BldKB, 602 aa. Contains 4x Pfam matches to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5, match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 3 4 18 22 [Reporter]SCO1108 (1G17)_PCR/7450 [Gene]SCO1108/6790 NC_003888 2SCG38.01c, possible regulatory protein(fragment), len: >106 aa; similar to N-terminal region of TR:CAC01635 (EMBL:AL391072) Streptomyces coelicolor putative regulatory protein SC9A4.08, 116 aa; fasta scores: opt: 218 z-score: 308.8 E(): 9.3e-10; 46.2% identity in 91 aa overlap,2SCG4.24c, hypothetical protein (fragment), len: >47 aa; similar to TR:CAB82870 (EMBL:AJ276673) Streptomyces coelicolor hypothetical 12.1 kD protein MmyJ, 111 aa; fasta scores: opt: 166 z-score: 266.6 E(): 2.3e-07; 68.4% identity in 38 aa overlap 3 4 18 21 [Reporter]SCO4159 (2C17)_PCR/7449 [Gene]SCO4159/6789 NC_003888 SCD84.26c, glnR, transcriptional regulatory protein, len: 267 aa; identical to previously sequenced SW:GLNR_STRCO (EMBL:L03213) Streptomyces coelicolor transcriptional regulatory protein GlnR. Contains Pfam match to entry PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 3 4 18 20 [Reporter]SCO6802 (3O13)_PCR/7448 [Gene]SCO6802/6788 NC_003888 SC1A2.11, possible DNA-binding protein, len: 192 aa. Similar to Streptomyces coelicolor TR:Q9X7W5 (EMBL:AL049485) putative DNA-binding protein, SC6A5.19 (204 aa), fasta scores opt: 213 z-score: 254.6 E(): 9e-07 30.9% identity in 178 aa overlap. Contains a Pfam match to entry PF01381 HTH_3, Helix-turn-helix with the putative helix-turn-helix motif situated between residues 28..49 (+7.43 SD) 3 4 18 19 [Reporter]SCO6209 (4K13)_PCR/7447 [Gene]SCO6209/6787 NC_003888 SC2G5.30, unknown, len: 191 aa; limited similarity to TR:O32141 (EMBL:Z99120) hypothetical protein from Bacillus subtilis (494 aa) fasta scores; opt: 204, z-score: 241.2,E(): 3.8e-06, (27.8% identity in 162 aa overlap). 3 4 18 18 [Reporter]SCO6381 (5G13)_PCR/7446 [Gene]SCO6381/6786 NC_003888 SC4A2.17c, probable lipoprotein, len: 447 aa; contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 3 4 18 17 [Reporter]SCO6548 (6C13)_PCR/7445 [Gene]SCO6548/6785 NC_003888 SC5C7.33, probable secreted cellulase, len: 579 aa; similar to many, e.g. TR:Q60029 (EMBL:U18978) beta-1,4-exocellulase precursor (596 aa), fasta scores; opt: 2131 z-score: 2323.9 E(): 0, 62.1% identity in 585 aa overlap. Contains possible N-terminal signal sequence, PS00655 Glycosyl hydrolases family 6 signature 1 and Pfam match to entry PF00553 CBD_1, Cellulose binding domain, score 161.20, E-value 9.8e-46 near C-terminus 3 4 18 16 [Reporter]SCO6780 (7O9)_PCR/7444 [Gene]SCO6780/6784 NC_003888 SC6A5.29, possible secreted protein, len: 401 aa. Contains possible N-terminal region signal peptide sequence 3 4 18 15 [Reporter]SCO2490 (8K9)_PCR/7443 [Gene]SCO2490/6783 NC_003888 SC7A8.29, probable dehydrogenase (putative secreted protein), len: 255 aa; similar to TR:O88068 (EMBL:AL031541) Streptomyces coelicolor putative dehydrogenase SCI35.33c, 260 aa; fasta scores: opt: 536 z-score: 554.4 E(): 1.7e-23; 40.0% identity in 255 aa overlap and to SW:DHG2_BACSU (EMBL:AB000617)Bacillus subtilis glucose 1-dehydrogenase II (EC 1.1.1.47) (GlcDH-II) (Gdh-II) (general stress protein 74) (Gsp74) YcdF, 258 aa; fasta scores: opt: 566 z-score: 584.7 E(): 3.5e-25; 39.3% identity in 252 aa overlap Contains two Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and possible N-terminal region signal peptide sequence 3 4 18 14 [Reporter]SCO5499 (9G9)_PCR/7442 [Gene]SCO5499/6782 NC_003888 SC8D9.11, probable Glu-tRNA-Gln amidotransferase subunit A, gatA, len: 497aa; similar to many eg. TR:O06491 (EMBL:AF008553) Glu-tRNAGln amidotransferase subunit A, gatA, from Bacillus subtilis (486 aa) fasta scores; opt: 1443, z-score: 1512.5, E(): 0, (52.0% identity in 465 aa overlap). 3 4 18 13 [Reporter]SCO7361 (10C9)_PCR/7441 [Gene]SCO7361/6781 NC_003888 SC9H11.15, possible DNA-binding protein, len: 509 aa. Previously sequenced: Streptomyces coelicolor TR:BAA82701 (EMBL:AB017438) Orf4 (509). Contains a putative helix-turn-helix motif situated between residues 439..460 (+3.90 SD). 3 4 18 12 [Reporter]SCO4374 (11O5)_PCR/7440 [Gene]SCO4374/6780 NC_003888 SCD10.06, possible integral membrane protein, len: 295 aa; similar to TR:Q9RUT6 (EMBL:AE001976) Deinococcus radiodurans PecM-related protein DR1295, 302 aa; fasta scores: opt: 769 z-score: 839.6 E(): 0; 47.1% identity in 291 aa overlap. Contains Pfam match to entry PF00892 DUF6, Integral membrane protein. Contains also possible hydrophobic membrane spanning regions 3 4 18 11 [Reporter]SCO3945 (12K5)_PCR/7439 [Gene]SCO3945/6779 NC_003888 SCD78.12, cydA, cytochrome oxidase subunit I len: 501 aa; similar to e.g. APPC_ECOLI (EMBL:S63811) cytochrome BD-II oxidase subunit I from Escherichia coli (485 aa), fasta scores opt: 1099 z-score: 1869.0 E():0 (48.6% identity in 510 aa overlap) and CYDA_BACSU (EMBL:D83026) cytochrome D ubiquinol oxidase subunit I from Bacillus subtilis (468 aa), fasta scores opt: 1178 z-score: 1729.6(43.9% identity in 472 aa overlap). Contains possible hydrophobic membrane spanning regions 3 4 18 10 [Reporter]SCO3066 (13G5)_PCR/7438 [Gene]SCO3066/6778 NC_003888 SCE25.07c, putative regulator of Sig15, len: 170 aa; identical to previously sequenced TR:CAB55834 (EMBL:AJ249581) Streptomyces coelicolor A3(2) putative regulator of Sig15, 170 aa 3 4 18 9 [Reporter]SCO1536 (14C5)_PCR/7437 [Gene]SCO1536/6777 NC_003888 SCL2.26c, hypothetical protein, len: 822 aa; similar to TR:O31530 (EMBL:Z99107) Bacillus subtilis YetA PROTEIN, 857 aa; fasta scores: opt: 982 z-score: 1078.1 E(): 0; 35.1% identity in 840 aa overlap 3 4 18 8 [Reporter]SCO3921 (15O1)_PCR/7436 [Gene]SCO3921/6776 NC_003888 SCQ11.04c, ptpA, low molecular weight protein-tyrosine-phosphatase, len: 164 aa; previously sequenced as SW:PTPA_STRCO (EMBL:U37580), ptpA, S.coelicolor low molecular weight protein-tyrosine-phosphatase (164 aa). Contains Pfam match to PF01451 LMWPc, Low molecular weight phosphotyrosine protein phosphatase 3 4 18 7 [Reporter]SCO0413 (16K1)_PCR/7435 [Gene]SCO0413/6775 NC_003888 SCF51.12c, unknown, len: 200 aa. 3 4 18 6 [Reporter]SCO0864 (17G1)_PCR/7434 [Gene]SCO0864/6774 NC_003888 SCM2.17c, probable ECF-family sigma factor, len: 198 aa. Highly similar to many sigma factors including: Streptomyces coelicolor TR:O86856 (EMBL:AJ007313) sigma factor SigT (236 aa), fasta scores opt: 268 z-score: 337.2 E(): 2.1e-11 34.3% identity in 181 aa overlap and Mycobacterium tuberculosis SW:RPSC_MYCTU (EMBL:Z73966) probable RNA polymerase sigma-C factor SigC (185 aa), fasta scores opt: 517 z-score: 643.3 E(): 1.9e-28 50.8% identity in 177 aa overlap. Note this CDS appears to be a duplicated on this cosmid and shares 68.5 % identity, in 197 aa overlap, with the downstream gene, SCM2.19c. Contains a Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily). 3 4 18 5 [Reporter]SCO3820 (18C1)_PCR/7433 [Gene]SCO3820/6773 NC_003888 SCGD3.21c, probable serine/threonine protein kinase, len: 522 aa; similar to many e.g. (EMBL:AF094711), PksC, Streptomyces coelicolor serine/threonine protein kinase (556 aa), fasta scores; opt: 907 z-score: 635.3 E(): 4.6e-28, 36.6% identity in 558 aa overlap, which lies upstream, on the opposite strand. Similar to mycobacterial putative serine/threonine protein kinases, but divergent at the C-terminal end e.g. TR:P71584 (EMBL:Z80233), PknB, Mycobacterium tuberculosis putative serine/threonine protein kinase (626 aa) (42.3% identity in 437 aa overlap). Contains a hydrophobic, possible membrane-spannig region. C-terminal region contains 5x DPTG repeats followed by 3x GG(E/D)PTG repeats. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain, PS00108 Serine/Threonine protein kinases active-site signature and PS00107 Protein kinases ATP-binding region signature 3 4 18 4 [Reporter]SCP1.187 (18O21)_PCR/7432 [Gene]SCP1.187/6772 NC_003888 SCP1.187, unknown, len: 183aa; 3 3 1 3 [Reporter]SCP1.221 (19G6)_PCR/5042 [Gene]SCP1.221/4599 NC_003888 SCP1.221, parA2, possible plasmid partitioning protein, len: 387aa; weakly similar to many eg. SW:P08866 (SOPA_ECOLI) sopA protein essential for F plasmid partitioning in Escherichia coli (388 aa) fasta scores; opt: 195, z-score: 215.5, E(): 2.9e-05, 27.7% identity in 213 aa overlap. Contains Pfam match to entry PF00991 ParA, ParA family ATPase. This CDS is directly duplicated at 221485..222648 (ParA1, SCP1.138) fasta scores; opt: 980, z-score: 954.5, E(): 0, 55.4% identity in 424 aa overlap. 3 4 18 3 [Reporter]SCP1.110 (19K21)_PCR/7431 [Gene]SCP1.110/6771 NC_003888 SCP1.110, possible ATP/GTP-binding protein, len: 333aa; N-terminal similar to that of TR:O69213 (EMBL:AJ224354) protein serine-threonine phosphatase from Anabaena sp. (strain PCC 7120) (858 aa) fasta scores; opt: 334, z-score: 391.4, E(): 2.5e-14, 42.0% identity in 150 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 2 3 23 22 [Reporter]SCO1760 (1J22)_PCR/5040 [Gene]SCO1760/4598 NC_003888 2SCI34.13c, probable cytidylate kinase, len: 231 aa; similar to SW:KCY_ECOLI (EMBL:X00785) Escherichia coli cytidylate kinase (EC 2.7.4.14) Cmk or MssA, 227 aa; fasta scores: opt: 469 z-score: 501.3 E(): 1.8e-20; 40.3% identity in 226 aa overlap. Contains Pfam match to entry PF02224 Cytidylate_kin, Cytidylate kinase and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 4 18 2 [Reporter]SCO5988 (20C21)_PCR/7430 [Gene]SCO5988/6770 NC_003888 SCBAC16H6.23, conserved hypothetical protein, len: 147aa: weakly similar to others eg. TR:Q9RSG5 (EMBL:AE002049) hypothetical protein from Deinococcus radiodurans (255 aa) fasta scores; opt: 118, Z-score: 140.3, 28.873% identity (32.540% ungapped) in 142 aa overlap. 2 3 23 21 [Reporter]SCO1439 (2F22)_PCR/5039 [Gene]SCO1439/4597 NC_003888 SC6D7A.02c, hisE, phosphoribosyl-ATP pyrophosphatase, len: 90 aa; similar to SW:HIS2_SULSO (EMBL:U82227) Sulfolobus solfataricus phosphoribosyl-ATP pyrophosphatase HisE (EC 3.6.1.31), 94 aa; fasta scores: opt: 175 z-score: 245.2 E(): 4e-06; 33.3% identity in 90 aa overlap and to SW:HIS2_MYCTU (EMBL:Z97559) Mycobacterium tuberculosis phosphoribosyl-ATP pyrophosphatase HisE (EC 3.6.1.31), 93 aa; fasta scores: opt: 336 z-score: 452.1 E(): 1.2e-17; 60.0% identity in 90 aa overlap. Contains Pfam match to entry PF01503 PRA-PH, Phosphoribosyl-ATP pyrophosphohydrolase 2 3 23 20 [Reporter]SCO7388 (3B22)_PCR/5038 [Gene]SCO7388/4596 NC_003888 SC10G8.15, possible dehydrogenase, len: 539 aa. Similar to many including: Synechocystis sp. TR:Q55808(EMBL:D64004) phytoene dehydrogenase (542 aa), fasta scores opt: 605 z-score: 655.2 E(): 4.5e-29 31.7% identity in 543 aa overlap and Rhodobacter sphaeroides SW:CRTD_RHOSH(EMBL:X63204) methoxyneurosporene dehydrogenase, CrtD (495 aa), fasta scores opt: 268 z-score: 292.4 E(): 7.2e-09 28.2% identity in 528 aa overlap. 2 3 23 19 [Reporter]SCO7612 (4N18)_PCR/5037 [Gene]SCO7612/4595 NC_003888 SC2H2.10c, hypothetical protein, len: 216 aa; similar to TR:O86515 (EMBL:AL031124) Streptomyces coelicolor hypothetical 20.0 kDa protein SC1C2.14c, 185 aa; fasta scores: opt: 633 z-score: 752.2 E(): 0; 50.0% identity in 184 aa overlap 2 3 23 18 [Reporter]SCO6704 (5J18)_PCR/5036 [Gene]SCO6704/4594 NC_003888 SC4C6.14, possible transcriptional regulator, len: 154 aa; similar to e.g. SW:MEXR_PSEAE (EMBL:U23763), MexR, Pseudomonas aeruginosa multidrug resistance operon repressor (147 aa), fasta scores; opt: 180 z-score: 234.3 E(): 1e-05, 30.7% identity in 114 aa overlap. C-terminal half shows highest similarity with other transcriptional regulators. C-terminal half similar to others from S. coelicolor e.g. TR:O86554 (EMBL:AL031350) S. coelicolor hypothetical protein (143 aa) (31.6% identity in 98 aa overlap). Could be a divergently-transcribed transcriptional regulator for the pca operon upstream of this CDS (very hypothetical). Contains Pfam match to entry PF01047 MarR, MarR family 2 3 23 17 [Reporter]SCO7556 (6F18)_PCR/5035 [Gene]SCO7556/4593 NC_003888 SC5F1.10, possible sugar transport permease, len: 331 aa; similar to TR:Q9X9R6 (EMBL:AJ009797) Streptomyces reticuli CebF protein, 305 aa; fasta scores: opt: 1258 z-score: 1401.2 E(): 0; 57.6% identity in 311 aa overlap and to TR:CAC10105 (EMBL:AL442165) Streptomyces coelicolor putative cellobiose transport permease 2SCC13.04, 337 aa; fasta scores: opt: 1343 z-score: 1424.3 E(): 0; 56.5% identity in 336 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 2 3 23 16 [Reporter]SCO2669 (7B18)_PCR/5034 [Gene]SCO2669/4592 NC_003888 SC6D10.12, unknown, len: 604 aa. Glycine and proline rich. Contains Pfam match to entry PF00498 FHA, FHA domain 2 3 23 15 [Reporter]SCO1995 (8N14)_PCR/5033 [Gene]SCO1995/4591 NC_003888 SC7H2.09c, unknown, len: 126aa; 2 1 12 20 [Reporter]SCO5770 (3F12)_PCR/765 [Gene]SCO5770/699 NC_003888 SC4H8.09, recX, len: 188 aa; similar to mycobacterial RecX proteins e.g. TR:E1173865 (EMBL:AL008967) MTV002.01c (174 aa), fasta scores; opt: 371 z-score: 436.7 E(): 3.9e-17, 42.7% identity in 164 aa overlap 2 3 23 14 [Reporter]SCO5847 (9J14)_PCR/5032 [Gene]SCO5847/4590 NC_003888 SC9B10.14, probable transcriptional regulator, len: 255 aa; weakly similar to many eg. FARR_ECOLI P13669 fatty acyl responsive regulator (240 aa), fasta scores; opt: 220 z-score: 345.1 E(): 4.7e-12, 28.0% identity in 236 aa overlap. Contains PS00043 Bacterial regulatory proteins, gntR family signature 2 1 12 19 [Reporter]SCO6024 (4B12)_PCR/764 [Gene]SCO6024/698 NC_003888 SC1C3.12, possible transferase, len: 697 aa; weakly similar to many putative polysaccharide biosynthesis proteins and to transferases e.g. Rhizobium leguminosarum TR:G2944086 (EMBL:AF050103) mannosyl transferase (352 aa), fasta scores; opt: 204 z-score: 193.1 E(): 0.0016, 28.0% identityin 239 aa overlap 2 1 12 18 [Reporter]SCO2078 (5N8)_PCR/763 [Gene]SCO2078/697 NC_003888 SC4A10.11c, putative membrane protein, len: 94 aa; unknown function, similar to hypothetical proteins e.g. TR:O06230 (EMBL:Z95388) Mycobacterium tuberculosis hypothetical protein (96 aa), fasta scores; opt: 262 z-score: 367.1 E(): 4e-13, 41.9% identity in 86 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 12 17 [Reporter]SCO7447 (6J8)_PCR/762 [Gene]SCO7447/696 NC_003888 SC5C11.04c possible acetyltranferase, len: 183 aa. Similar to many including: Proteus mirabilis TR:O86434(EMBL:AJ000084) putative acetyl transferase (185 aa), fasta scores opt: 428 z-score: 512.9 E(): 3.8e-21 43.6% identity in 179 aa overlap. Contains a Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 2 1 12 16 [Reporter]SCO7255 (7F8)_PCR/761 [Gene]SCO7255/695 NC_003888 SC5H1.37, hypothetical protein, len: 364 aa; unknown function, similar to TR:P73120 (EMBL:D90903) Synechocystis sp. hypothetical protein (358 aa), fasta scores; opt: 492 z-score: 559.6 E(): 7.6e-24, 31.6% identity in 364 aa overlap 2 1 12 15 [Reporter]SCO5331 (8B8)_PCR/760 [Gene]SCO5331/694 NC_003888 SC6G9.02, possible DNA methylase, len: 1200 aa; highly similar to TR:O86561 (EMBL:AL031350) (71.5% identity in 867 aa overlap) and TR:O86674 (EMBL:AL031371) (90.8% identity in 370 aa overlap) which together constitute a single hypothetical protein split across the join between S.coelicolor cosmids 1F2 and 4G2. It has very weak similarity to DNA methylases, which combined with the presence of a DNA methylases signature and possible DNA-binding region (helix-turn-helix) provides circumstantial evidence of DNA-methylase function. The central third of the CDS is relatively AT rich. Contains probable helix-turn-helix motif at aa 38-59 (Score 1036, +2.72 SD). Contains PS00092 N-6 Adenine-specific DNA methylases signature 2 1 12 14 [Reporter]SCO7819 (9N4)_PCR/759 [Gene]SCO7819/693 NC_003888 SC8E7.16c, possible integral membrane protein, len: 282 aa. Similar to the several e.g. Myxococcus xanthus SW:GUFA_MYXXA(EMBL:X71062) integral membrane protein found within the carotenogenesis operon, GufA (254 aa), fasta scores opt: 173 z-score: 197.7 E(): 0.001625.8% identity in 252 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 2 1 12 12 [Reporter]SCO2272 (11F4)_PCR/757 [Gene]SCO2272/692 NC_003888 SCC75A.18, binding-protein-dependent transport lipoprotein, len: 350 aa. Similar to many proteins involved in iron acquisition including: Yersinia pestis SW:HMUT_YERPE(EMBL:U60647) hemin-binding periplasmic protein HmuT precursor (278 aa), fasta scores opt: 462 z-score: 474.0 E(): 5e-19 33.3% identity in 258 aa overlap and Corynebacterium diphtheriae TR:AAD37493(EMBL:AF109162) probable lipoprotein heme receptor HmuT (353 aa), fasta scores opt: 670 z-score: 680.9 E(): 1.5e-30 37.2% identity in 339 aa overlap. Contains a Pfam match to entry PF01497 Peripla_BP_2, Periplasmic binding protein and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains a possible N-terminal signal sequence. 2 1 12 11 [Reporter]SCO4784 (12B4)_PCR/756 [Gene]SCO4784/691 NC_003888 SCD63.16c, hypothetical protein, len: 110 aa; similar to SW:Y948_MYCTU (EMBL:Z79700) Mycobacterium tuberculosis hypothetical 11.8 kD protein RV0984c, MTCY10D7.26, 105 aa; fasta scores: opt: 195 z-score: 252.8 E(): 1.2e-06; 47.2% identity in 72 aa overlap 2 1 12 10 [Reporter]SCO2847 (12N24)_PCR/755 [Gene]SCO2847/690 NC_003888 SCE20.21, possible oxidoreductase, len: 360 aa. Similar to several other putative oxidoreductases e.g. Escherichia coli SW:YDGJ_ECOLI(EMBL:AE000258) hypothetical oxidoreductase (346 aa), fasta scores opt: 873 z-score: 972.9 E():0 45.0% identity in 353 aa overlap. Contains a Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family. 3 4 17 22 [Reporter]SCO1018 (1C17)_PCR/7428 [Gene]SCO1018/6769 NC_003888 2SCG2.32c, putative glutamate racemase, len: 280 aa; similar to SW:MURI_ECOLI (EMBL:) Escherichia coli glutamate racemase (EC 5.1.1.3) MurI, 285 aa; fasta scores: opt: 359 z-score: 390.2 E(): 3.3e-14; 34.4% identity in 256 aa overlap. Contains Pfam match to entry PF01177 Asp_Glu_race, Aspartate / glutamate racemase and match to Prosite entry PS00923 Aspartate and glutamate racemases signature 1 3 4 17 21 [Reporter]SCO0379 (2O13)_PCR/7427 [Gene]SCO0379/6768 NC_003888 SCF62.05, katA, catalase (EC 1.11.1.6) len: 487 aa. Has been previously sequenced and characterised from Streptomyces coelicolor strain ATCC10147 TR:P77948 (EMBL; X96981) catalase (EC 1.11.1.6) (488 aa), fasta scores opt: 3300 z-score: 3768.6 E():0 98.2% identity in 488 aa overlap. Also similar to another proposed Streptomyces coelicolor catalase (EC 1.11.1.6) TR:Q9Z598 (EMBL; AL035478) SC2G5.25C (487 aa), fasta scores opt: 1704 z-score: 1896.1 E():0 53.8% identity in 483 aa overlap. Contains Prosite hits to PS00437 Catalase proximal heme-ligand signature and to PS00438 Catalase proximal active site signature. Also contains a Pfam match to entry PF00199 catalase, Catalase. 3 4 17 19 [Reporter]SCO5478 (4G13)_PCR/7425 [Gene]SCO5478/6767 NC_003888 SC2A11.12, probable oligopeptide transport system integral membrane protein, len: 332 aa; similar to many e.g. OPPB_ECOLI oligopeptide transport system permease protein (306 aa), fasta scores; opt: 568 z-score: 807.4 E(): 0, 35.0% identity in 337 aa overlap Also similar to S. coelicolor TR:P72408 (EMBL:U68036) bldKC (323 aa), fasta scores; opt: 875 z-score: 1186.1 E(): 0 42.4% identity in 337 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature and Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 34.10, E-value 3.1e-06 3 4 17 18 [Reporter]SCO6376 (5C13)_PCR/7424 [Gene]SCO6376/6766 NC_003888 SC4A2.12, probable integral membrane protein, len: 299 aa 3 4 17 17 [Reporter]SCO7460 (6O9)_PCR/7423 [Gene]SCO7460/6765 NC_003888 SC5C11.17, possible lipoprotein, len: 356 aa. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 3 4 17 16 [Reporter]SCO6776 (7K9)_PCR/7422 [Gene]SCO6776/6764 NC_003888 SC6A5.25, unknown, len: 370 aa; similar to SW:YZ34_MYCTU hypothetical protein from Mycobacterium tuberculosis (372 aa) fasta scores; opt: 1125, z-score: 1301.0, E(): 0, (51.3% identity in 335 aa overlap). Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) 3 4 17 15 [Reporter]SCO5316 (8G9)_PCR/7421 [Gene]SCO5316/6763 NC_003888 SC6G9.17, acyl carrier protein, len 90 aa; whiE locus acyl carrier protein previously sequenced from S.coelicolor as SW:ACP_STRCO (EMBL:X55942). Contains Pfam match to entry PF00550 pp-binding, Phosphopantetheine attachment site and PS00012 Phosphopantetheine attachment site 3 4 17 14 [Reporter]SCO5495 (9C9)_PCR/7420 [Gene]SCO5495/6762 NC_003888 SC8D9.07, possible membrane associated phosphodiesterase, len: 746 aa; similar to many eg. TR:O87376 (EMBL:AF052518) c-di-GMP phosphodiesterase A from Acetobacter xylinus cdg2 operon (752 aa) fasta scores; opt: 959, z-score: 1059.2, E(): 0, (31.7% identity in 603 aa overlap). Appears to have two distinct regions with the first 300aa containing several potential membrane spanning hydrophobic regions and the remainder of the protein showing similarity to the phosphodiesterase mentioned above. Also similar to a nearby gene on the same cosmid (SC8D9.22) fasta scores; opt: 736, z-score: 625.6, E(): 5.3e-30, (32.6% identity in 729 aa overlap). Contains TTA Leu codon, possible target for bldA regulation. Also contains Pfam match to entry PF00990 DUF9, Domain of unknown function, score 166.70, E-value 3.9e-46 and Pfam match to entry PF00563 DUF2, Domain of unknown function 2, score 291.30, E-value 1.2e-83. Contains possible hydrophobic membrane spanning regions 2 3 23 13 [Reporter]SCO1935 (10F14)_PCR/5031 [Gene]SCO1935/4589 NC_003888 SCC22.17, tktA1, probable transketolase, len: 695aa; similar to many egs. TR:O88019 (EMBL:AL031107) tkt, transketolase from Streptomyces coelicolor (698 aa) fasta scores; opt: 3653, z-score: 4066.7, E(): 0, (79.1% identity in 685 aa overlap) and TR:CAB19861 (EMBL:AB023377) glutamicum transketolase from Brevibacterium lactofermentum (676 aa) fasta scores; opt: 2710, z-score: 3016.8, E(): 0, (60.7% identity in 684 aa overlap). (Note Streptomyces coelicolor has another probable transketolase, tkt2, TR:Q9ZC16 (EMBL:AL033505) which is more similar to eukaryotic homologues.) Contains Pfam match to entry PF00456 transketolase, Transketolase and Prosite matches to PS00801 Transketolase signature 1 and PS00802 Transketolase signature 2. 3 4 17 13 [Reporter]SCO2530 (10O5)_PCR/7419 [Gene]SCO2530/6761 NC_003888 SCC117.03, unknown, len: 88 aa. 2 3 23 12 [Reporter]SCO4099 (11B14)_PCR/5030 [Gene]SCO4099/4588 NC_003888 SCD17.03, probable tetR family transcriptional regulator, len: 216 aa. Similar to many e.g. Propionibacterium freudenreichii TR:P72185 (EMBL:U51164) repressor protein (HemR) (259 aa), fasta scores opt: 250 z-score: 297.6 E(): 3.2e-09 36.7% identity in 147 aa overlap. Also similar to several Streptomyces coelicolor putative regulators e.g. TR:O54180 (EMBL:AL021411) SC7H1.21 (203 aa), fasta scores opt: 330 z-score: 391.0 E(): 2e-14 40.0% identity in 220 aa overlap and SC5G9.05 (193 aa), fasta scores opt: 292 z-score: 269.9 E(): 9.2e-10 40.9% identity in 149 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a probable helix-turn-helix motif between residues 47..68 (+3.70 SD). 3 4 17 12 [Reporter]SCO5288 (11K5)_PCR/7418 [Gene]SCO5288/6760 NC_003888 SCCB12.12c, unknown, len: 263 aa 2 3 23 11 [Reporter]SCO4152 (12N10)_PCR/5029 [Gene]SCO4152/4587 NC_003888 SCD84.19, possible secreted 5'-nucleotidase, len: 612 aa; similar to SW:5NTD_DISOM (EMBL:X62278) Discopyge ommata 5'-nucleotidase precursor (EC 3.1.3.5) (ecto-nucleotidase), 577 aa; fasta scores: opt: 322 z-score: 332.6 E(): 4.6e-11; 24.6% identity in 574 aa overlap. Contains Pfam match to entry PF01009 5_nucleotidase, 5'-nucleotidase and possible N-terminal region signal peptide sequence 2 3 23 10 [Reporter]SCO3115 (13J10)_PCR/5028 [Gene]SCO3115/4586 NC_003888 SCE41.24c, unknown, len: 889 aa. High content in alanine, glycine and proline amino acid residues. Contains 20x degenerate repeat: (G/P/A/N)P(G/P/A) 2 3 23 8 [Reporter]SCO3694 (15B10)_PCR/5026 [Gene]SCO3694/4585 NC_003888 SCH35.30, possible transcriptional regulator, len: 124aa; similar to many eg. TR:CAA09641 (EMBL:AJ011500) putative transcriptional regulator from Streptomyces violaceoruber (196 aa) fasta scores; opt: 206, z-score: 269.5, E(): 1.1e-07, (31.2% identity in 109 aa overlap). Contains probable helix-turn-helix at 2-23aa (+3.36 SD) 2 3 23 7 [Reporter]SCO0854 (16N6)_PCR/5025 [Gene]SCO0854/4584 NC_003888 SCM2.07, unknown, len: 863 aa. 2 3 23 6 [Reporter]SCO2900 (17J6)_PCR/5024 [Gene]SCO2900/4583 NC_003888 SCE6.37c, possible membrane protein, len: 110 aa. Contains a possible membrane spanning hydrophobic domain. 2 3 23 5 [Reporter]SCO3183 (18F6)_PCR/5023 [Gene]SCO3183/4582 NC_003888 SCE87.34, unknown, len: 204 aa. Similar to many proteins of undefined function including: Escherichia coli SW:YGFA_ECOLI (EMBL:M12965) hypothetical 21.1 KD protein in SSR-SERA intergenic region (182 aa), fasta scores opt: 298 z-score: 347.9 E(): 5.3e-12 35.0% identity in 180 aa overlap. Also weakly similar to several Eukaryotic proteins such as: Homo sapiens SW:FTHC_HUMAN (EMBL: L38928) 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) (202 aa), fasta scores opt: 183 z-score: 217.8 E(): 9.3e-05 23.4% identity in 188 aa overlap. Contains a Pfam match to entry PF01812 5-FTHF_cyc-lig. 2 3 23 4 [Reporter]SCO1976 (19B6)_PCR/5022 [Gene]SCO1976/4581 NC_003888 SC3C9.11, possible integral membrane protein, len: 159 aa; similar to TR:BAB50721 (EMBL:AP003003) Rhizobium loti MLR3943 protein, 158 aa; fasta scores: opt: 409 Z-score: 476.8 bits: 94.4 E(): 6e-19; 40.667% identity in 150 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 12 9 [Reporter]SCO0459 (13J24)_PCR/754 [Gene]SCO0459/689 NC_003888 SCF51A.37, unknown, len: 259 aa. Similar to Mycobacterium tuberculosis SW:YX26_MYCTU (EMBL; Z74024) hypothetical 30.6 KD protein CY274.26C (283 aa), fasta scores opt: 268 z-score: 318.2 E(): 2.3e-10 28.9% identity in 263 aa overlap. 2 3 23 3 [Reporter]SCP1.194 (20N2)_PCR/5021 [Gene]SCP1.194/4580 NC_003888 SCP1.194, unknown, len: 185aa; 2 1 12 8 [Reporter]SCO1571 (14F24)_PCR/753 [Gene]SCO1571/688 NC_003888 SCL24.07, unknown, len: 195 aa 2 1 12 7 [Reporter]SCO1887 (15B24)_PCR/752 [Gene]SCO1887/687 NC_003888 SCI7.05c, possible integral membrane transport protein, len: 298aa; similar to many eg. TR:O51924 (EMBL:AF012836) inner membrane protein, MalF from the trehalose/maltose transport system of the hyperthermophilic archaeon Thermococcus litoralis (300 aa) fasta scores; opt: 681, z-score: 814.3, E(): 0, (37.5% identity in 285 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible membrane spanning hydrophobic regions. 2 1 12 6 [Reporter]SCO3431 (16N20)_PCR/751 [Gene]SCO3431/686 NC_003888 SCE9.38, possible membrane protein, len: 244 aa; unknown function, contains a hydrophobic, possible membrane-spanning region. Contains PS00213 Lipocalin signature, so may be involved in transport of small, hydrophobic molecules 2 1 12 5 [Reporter]SCO5597 (17J20)_PCR/750 [Gene]SCO5597/685 NC_003888 SC2E1.14, sip2, probable signal peptidase I, len: 446 aa; similar to many e.g. LEP_HAEIN signal peptidase I (EC 3.4.21.89) (349 aa), fasta scores; opt: 151, z-score: 317.3 E(): 1.9e-10, 30.1% identity in 286 aa overlap. Contains PS00761 Signal peptidases I signature 3, Pfam match to entry signal_pept_I PF00461, Signal peptidases I, score 42.41 and probable transmembrane domain. Also similar to surrounding genes sip1 (E(): 9.7e-24, 44.9% identity in 274 aa overlap), sip3 (E(): 1.8e-28, 56.3% identity in 245 aa overlap), and sip4 (E(): 1.1e-09, 35.9% identity in 223 aa overlap). Note that after aa 325 the ORF overlaps the downstream sip3 gene SC2E1.15, and positional base preference suggests that translation is in the other frame. No error in the cosmid sequence can be found to account for this. Contains possible hydrophobic membrane spanning region 2 1 12 4 [Reporter]SCO0389 (18F20)_PCR/749 [Gene]SCO0389/684 NC_003888 SCF62.15, possible lipoprotein, len: 511 aa. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 2 1 12 3 [Reporter]SCP1.16c (19B20)_PCR/748 [Gene]SCP1.16c/683 NC_003888 SCP1.16c, unknown, len: 194aa; 2 1 12 2 [Reporter]SCO3058 (20J16)_PCR/747 [Gene]SCO3058/682 NC_003888 SCBAC19G2.13c, possible dipeptidase, len: 400 aa: similar to many eukaryotic peptidases e.g. SW:P31429 (MDP1_RABIT) rabbit renal dipeptidase (410 aa) fasta scores; opt: 788, Z-score: 879.1, 42.432% identity (48.580% ungapped) in 403 aa overlap. Also weakly similar to TR:Q9KH70 (EMBL:AF268476) thermostable dipeptidase from Brevibacillus borstelensis (307 aa) fasta scores; opt: 450, Z-score: 506.1, 31.034% identity (36.486% ungapped) in 348 aa overlap. Contains Pfam match to entry PF01244 Renal_dipeptase, Renal dipeptidase. Also similar to neighbouring downstream CDS SCBAC19G2.12c 2 1 11 22 [Reporter]SCO1270 (1J12)_PCR/745 [Gene]SCO1270/681 NC_003888 2SCG18.17c, probable pyruvate dehydrogenase alpha subunit, len: 323 aa; similar to TR:O69011 (EMBL:AF086791) Zymomonas mobilis pyruvate dehydrogenase alpha subunit PdhA, 353 aa; fasta scores: opt: 553 z-score: 643.8 E(): 2.3e-28; 33.4% identity in 314 aa overlap. Contains Pfam match to entry PF00676 E1_dehydrog, Dehydrogenase E1 component 2 1 11 21 [Reporter]SCO2640 (2F12)_PCR/744 [Gene]SCO2640/680 NC_003888 SC8E4A.10, asd1, aspartate semialdehyde dehydrogenase, len: 340 aa; highly similar to SW:DHAS_STRAK (EMBL:U29446) Streptomyces akiyoshiensis aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) Asd, 338 aa; fasta scores: opt: 1879 z-score: 2082.0 E(): 0; 88.4% identity in 336 aa overlap and similar to TR:Q9XAI8 (EMBL:AL079348) S. coelicolor SC66T3.25c, 362 aa; fasta scores: opt: 979 z-score: 1005.7 E(): 0; 49.1% identity in 346 aa overlap. Contains Pfam match to entry PF01118 Semialdhyde_dh, Semialdehyde dehydrogenase and match to Prosite entry PS01103 Aspartate-semialdehyde dehydrogenase signature 3 4 17 11 [Reporter]SCO4798 (12G5)_PCR/7417 [Gene]SCO4798/6759 NC_003888 SCD63A.09c, possible peptidase, len: 565 aa; C-terminal region similar to SW:LSTP_STAST (EMBL:X06121) Staphylococcus staphylolyticus lysostaphin precursor (EC 3.4.24.75) Lss, 480 aa; fasta scores: opt: 299 z-score: 293.2 E(): 7.2e-09; 25.0% identity in 368 aa overlap and to TR:O50513 (EMBL:AL009204) Streptomyces coelicolor hypothetical protein SC9B10.06, 250 aa; fasta scores: opt: 458 z-score: 352.7 E(): 4.3e-14; 41.6% identity in 231 aa overlap. Contains Pfam match to entry PF01551 Peptidase_M37, Peptidase family M23/M37 3 4 17 10 [Reporter]SCO3062 (13C5)_PCR/7416 [Gene]SCO3062/6758 NC_003888 SCE25.03c, probable two-component system histidine kinase, len: 424 aa; similar to TR:O86661 (EMBL:AL031182) Streptomyces coelicolor putative two-component sensor SC4A2.05, 436 aa; fasta scores: opt: 486 z-score: 535.4 E(): 2.3e-22; 31.8% identity in 450 aa overlap. Contains Pfam match to entry PF00512 signal, Histidine kinase. Contains possible hydrophobic membrane spanning regions 3 4 17 9 [Reporter]SCO1649 (14O1)_PCR/7415 [Gene]SCO1649/6757 NC_003888 SCI41.32, putative ferredoxin, len: 101 aa. Identical to TR:087593 (EMBL:AF086832) Streptomyces coelicolor putative ferredoxin, len: 101 aa and similar to SW:FER_BACST (SW:P00212) Bacillus stearothermophilus ferredoxin, fasta scores: opt: 153 z-score: 211.3 E(): 0.00021; 33.3% identity in 69 aa overlap 3 4 17 8 [Reporter]SCO1231 (15K1)_PCR/7414 [Gene]SCO1231/6756 NC_003888 2SCG1.06c, ureD, urease accessory protein, len: 254 aa; similar to SW:URED_ECOLI (EMBL:L03307) Escherichia coli urease accessory protein UreD, 278 aa; fasta scores: opt: 139 z-score: 156.7 E(): 0.29; 28.9% identity in 166 aa overlap. Contains Pfam match to entry PF01774 UreD, UreD urease accessory protein 3 4 17 7 [Reporter]SCO2894 (16G1)_PCR/7413 [Gene]SCO2894/6755 NC_003888 SCE6.31c, possible ABC-transport protein, ATP-binding component, len: 354 aa. Similar to many other proposed transport proteins e.g. Synechocystis sp. (strain PCC 6803) TR:P73329(EMBL:D90905) ABC transporter (326 aa), fasta scores opt: 831 z-score: 906.9 E(): 0 45.3% identity in 296 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop) and a Pfam match to entry PF00005 ABC_tran, ABC transporter. 3 4 17 6 [Reporter]SCO3702 (17C1)_PCR/7412 [Gene]SCO3702/6754 NC_003888 SCH35.22c, possible DNA-binding protein, len: 215aa; similar to TR:Q51078 (EMBL:Z21683) hypothetical DNA-binding protein from Streptomyces lactamdurans (214 aa) fasta scores; opt: 1210, z-score: 1460.4, E(): 0, 77.2% identity in 215 aa overlap 3 4 17 5 [Reporter]SCO0764 (17O21)_PCR/7411 [Gene]SCO0764/6753 NC_003888 SCF81.23c, possible hydrolase, len: 536 aa; low similarity to SW:E13B_OERXA (EMBL:M60826) Oerskovia xanthineolytica glucan endo-1,3-beta-glucosidase precursor (EC 3.2.1.39) ((1->3)-beta-glucan endohydrolase) ((1->3)-beta-glucanase) 548 aa; fasta scores: opt: 190 z-score: 208.5 E(): 0.00031; 28.3% identity in 166 aa overlap 3 4 17 4 [Reporter]SCP1.115 (18K21)_PCR/7410 [Gene]SCP1.115/6752 NC_003888 SCP1.115, unknown, len: 175aa; weak similarity with TR:Q9Z6E9 (EMBL:AF073300) WhiB, putative regulatory protein from Mycobacterium smegmatis (96 aa) fasta scores; opt: 118, z-score: 151.2, E(): 0.59, 28.9% identity in 97 aa overlap. 2 3 22 22 [Reporter]SCO1085 (1F22)_PCR/5019 [Gene]SCO1085/4579 NC_003888 2SCG4.01, probable acyltransferase, len: 242 aa; similar to TR:O0780 (EMBL:Z97188) Mycobacterium tuberculosis acyltransferase MTCY409.14, 259 aa; fasta scores: opt: 758 z-score: 928.7 E(): 0; 51.7% identity in 234 aa overlap and to SW:PLSC_LIMDO (EMBL:X83266) Limnanthes douglasii 1-acyl-SN-glycerol-3-phosphate acyltransferase (EC 2.3.1.51) PlsC, 281 aa; fasta scores: opt: 218 z-score: 272.2 E(): 1.1e-07; 31.0% identity in 174 aa overlap. Contains Pfam match to entry PF01553 Acyltransferase, Acyltransferase 3 4 17 3 [Reporter]SCO5337 (19G21)_PCR/7409 [Gene]SCO5337/6751 NC_003888 SCBAC5H2.06c, unknown, len: 243 aa 2 3 22 21 [Reporter]SCO6031 (2B22)_PCR/5018 [Gene]SCO6031/4578 NC_003888 SC1C3.19, hemE, uroporphyrinogen decarboxylase, len: 355 aa; highly similar to many e.g. DCUP_ECOLI uroporphyrinogen decarboxylase (EC 4.1.1.37) (354 aa), fasta scores; opt: 879 z-score: 1073.9 E(): 0, 42.3% identity in 345 aa overlap. Contains PS00906 and PS00907 Uroporphyrinogen decarboxylase signatures 1 and 2 3 4 17 2 [Reporter]SCO3049 (20O17)_PCR/7408 [Gene]SCO3049/6750 NC_003888 SCBAC19G2.04, possible acyl-CoA hydrolase, len: 197 aa: similar to many e.g. SW:Q64559 (CTE2_RAT) rat brain cytosolic acyl-coenzyme A thioester hydrolase (338 aa) fasta scores; opt: 353, Z-score: 394.1, 34.973% identity (35.754% ungapped) in 183 aa overlap and TR:Q9I0E9 (EMBL:AE004697) hypothetical protein from Pseudomonas aeruginosa (166 aa) fasta scores; opt: 391, Z-score: 440.1, 42.949% identity (44.667% ungapped) in 156 aa overlap. Contains Pfam match to entry PF01662 Acyl-CoA_hydro, Cytosolic long-chain acyl-CoA thioester hydrolase. 2 3 22 20 [Reporter]SCO6424 (3N18)_PCR/5017 [Gene]SCO6424/4577 NC_003888 SC1A6.13c, probable two-component system sensor protein, len: 331 aa; contains two potential transmembrane domains in the C-terminus; similar to e.g. DEGS_BACSU P13799 sensor protein DegS (EC 2.7.3.-) Bacillus subtilis (385 aa), fasta scores: opt: 316 z-score: 369.3 E(): 1.9e-13, 30.7% identity in 212 aa overlap and to TR:Q52558 (EMBL:U02041) Burkholderia solanacearum VrsA, 502 aa; fasta scores: opt: 398 Z-score: 416.9 E(): 1.4e-15; 31.724% identity in 290 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 22 19 [Reporter]SCO7608 (4J18)_PCR/5016 [Gene]SCO7608/4576 NC_003888 SC2H2.06, hypothetical protein, len: 150 aa; similar to TR:Q9L0J1 (EMBL:AL161691) Streptomyces coelicolor hypothetical 15.7 kDa protein SCD40A.20, 145 aa; fasta scores: opt: 335 z-score: 414.8 E(): 1.4e-15; 45.0% identity in 120 aa overlap 2 3 22 18 [Reporter]SCO6827 (5F18)_PCR/5015 [Gene]SCO6827/4575 NC_003888 SC4A9.04c, polyketide synthase, len: 2358 aa. Highly similar to many including: Stigmatella aurantiaca TR:AAF19813(EMBL:AF188287) polyketide synthase, MtAE, found within the Myxothiazol biosynthetic gene cluster (1947 aa), fasta scores opt: 2671 z-score: 2436.5 E():0 35.8% identity in 1770 aa overlap and Mycobacterium tuberculosis SW:PPSA_MYCTU(EMBL:Z74697) phenolpthiocerol synthesis polyketide synthase gene, PpsA (1876 aa), fasta scores opt: 2221 z-score: 2025.5 E(): 0 33.6% identity in 1761 aa overlap. Contains Prosite hits to PS00606 Beta-ketoacyl synthases active site and 2xPS00012 Phosphopantetheine attachment site. Also contains Pfam matches to entries PF00109 ketoacyl-synt, Beta-ketoacyl synthase, PF00698 Acyl_transf, Acyl transferase domain, PF00107 adh_zinc, Zinc-binding dehydrogenases and 2xPF00550 pp-binding, Phosphopantetheine attachment site. The 72 amino acids, 2139..2210, are perfectly repeated between residues 2280..2351 in the C-terminus of this protein. 2 3 22 17 [Reporter]SCO7552 (6B18)_PCR/5014 [Gene]SCO7552/4574 NC_003888 SC5F1.06c, possible tetR-family transcriptional regulator, len: 208 aa; similar to many Streptomyces coelicolor tetR-family transcriptional regulators, e.g. TR:Q9WX19 (EMBL:AL079345) putative transcriptional regulator SCE68.13, 230 aa; fasta scores: opt: 567 z-score: 703.0 E(): 1.3e-31; 44.9% identity in 205 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Also contains possible helix-turn-helix motif at residues 38..59 (+5.63 SD) 2 3 22 16 [Reporter]SCO1413 (7N14)_PCR/5013 [Gene]SCO1413/4573 NC_003888 SC6D7.26, unknown, len: 472 aa. 2 2 6 6 [Reporter]SCO0232 (16F15)_PCR/2635 [Gene]SCO0232/2399 NC_003888 SCJ9A.11c, unknown, len: 61aa; predicted by GC Frameplot, Hidden Markov Model and amino acid usage. Similar to a region of several aldolases eg. TR:AAB40121 (EMBL:U70664) from Haloferax sp. (215 aa) fasta scores; opt: 140, z-score: 198.7, E(): 0.0011, (49.0% identity in 51 aa overlap). 2 3 22 15 [Reporter]SCO1991 (8J14)_PCR/5012 [Gene]SCO1991/4572 NC_003888 SC7H2.05, hypothetical protein, len: 186 aa; similar to SW:YRFH_ECOLI hypothetical protein from Escherichia coli (133 aa) fasta scores; opt: 228, z-score: 249.5, E(): 1.4e-06, (35.2% identity in 122 aa overlap). 2 2 6 5 [Reporter]SCO3161 (17B15)_PCR/2634 [Gene]SCO3161/2398 NC_003888 SCE87.12, possible ABC transport protein, ATP-binding subunit, len: 260 aa. Highly similar to many cbiO-like proteins thought to be involved in ATP-dependent cobalt transport e.g. Methanococcus jannaschii TR:Q58488(EMBL:U67551) cobalt transport ATP-binding protein (279 aa), fasta scores opt: 737 z-score: 823.3 E(): 0 45.8% identity in 238 aa overlap and Streptomyces coelicolor TR:O54187 (EMBL:AL021411) cobalt transport system ATP binding protein SC7H1.28c (284 aa), fasta scores opt: 664 z-score: 742.6 E():0 44.4% identity in 261 aa overlap. Contains a possible membrane spanning hydrophobic domain and a Pfam match to entry PF00005 ABC_tran, ABC transporter. Also contains Prosite hits to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 2 3 22 14 [Reporter]SCO7528 (9F14)_PCR/5011 [Gene]SCO7528/4571 NC_003888 SC8G12.04, unknown, len: 166 aa; similar to TR:Q9KZG9 (EMBL:AL353864) hypothetical protein SC8F11.09 from Streptomyces coelicolor (137 aa) fasta scores; opt: 348, Z-score: 421.0, E(): 8.2e-16, 38.060% identity (38.636% ungapped) in 134 aa overlap. 2 1 11 20 [Reporter]SCO6683 (3B12)_PCR/743 [Gene]SCO6683/679 NC_003888 SC5A7.33, ramA, probable ABC transporter, len: 636 aa; N-terminus contains transmembrane domain, C-terminus contains ATP-binding domain. Almost identical to TR:Q53818 (EMBL:U03771) potential ATP-binding membrane transport protein RamA (636 aa)(99.8% identity in 636 aa overlap)Y08A_MYCTU hypothetical ABC transporter ATP-binding protein MTCY50.10 (631 aa), fasta scores; opt: 556 z-score: 414.6 E(): 7.4e-16, 28.1% identity in 626 aa overlap. Contains sixteen degenerate GA repeats at C-terminus, PS00017 ATP /GTP-binding match to entry PF00005 ABC_tran, ABC transporters, score 139.50, E-value 6e-38. Some similarity to RamB (E(): 3.7e-05, 32.7% identity in 627 aa overlap) 2 2 6 4 [Reporter]SCO1063 (18N11)_PCR/2633 [Gene]SCO1063/2397 NC_003888 SCG22.09c, possible sugar transport integral membrane protein, len: 297 aa; similar to SW:LACG_AGRRD (EMBL:X66596) Agrobacterium radiobacter lactose transport system permease protein LacG, 273 aa; fasta scores: opt: 514 z-score: 627.5 E(): 1.8e-27; 35.1% identity in 282 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Contains also possible hydrophobic membrane spanning regions 2 3 22 13 [Reporter]SCO1931 (10B14)_PCR/5010 [Gene]SCO1931/4570 NC_003888 SCC22.13c, unknown, len: 259aa; 2 1 11 19 [Reporter]SCO6933 (4N8)_PCR/742 [Gene]SCO6933/678 NC_003888 SC1G8.05, unknown, len: 149 aa. 2 2 6 3 [Reporter]SCO1969 (19J11)_PCR/2632 [Gene]SCO1969/2396 NC_003888 SC3C9.04, possible DNA-methyltransferase, len: 184 aa; similar to SW:OGT_MYCPA (EMBL:AJ011838) Mycobacterium paratuberculosis methylated-DNA protein-cysteine methyltransferase (EC 2.1.1.63) Ogt, 165 aa; fasta scores: opt: 355 Z-score: 399.1 bits: 80.3 E(): 1.3e-14; 40.964% identity in 166 aa overlap. Contains Pfam matches to entries PF02870 Methyltransf_1N, 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain and PF01035 Methyltransf_1, 6-O-methylguanine DNA methyltransferase, DNA binding domain and match to Prosite entry PS00374 Methylated-DNA--protein-cysteine methyltransferase active site 2 1 11 18 [Reporter]SCO2074 (5J8)_PCR/741 [Gene]SCO2074/677 NC_003888 SC4A10.07c, probable signal peptidase, len: 204 aa; similar to many e.g. SW:LSPA_PSEFL (EMBL:M35366), LspA, Pseudomonas fluorescens lipoprotein signal peptidase (170 aa), fasta scores; opt: 250 z-score: 309.9 E(): 6.2e-10, 36.4% identity in 165 aa overlap, but contains an approx. 40 aa N-terminal extension not present in similar sequences. Contains Pfam match to entry PF01252 Peptidase_A8, Signal peptidase (SPase) II and possible hydrophobic membrane spanning regions 2 2 6 2 [Reporter]SCP1.270 (20B11)_PCR/2631 [Gene]SCP1.270/2395 NC_003888 SCP1.270, unknown, doubtful CDS, len: 96aa; 2 1 11 17 [Reporter]SCO2061 (6F8)_PCR/740 [Gene]SCO2061/676 NC_003888 SC4G6.30c, possible ABC transport ATP-binding subunit, len: 324aa; similar to many eg. TR:Q53716 (EMBL:L06249) ATP-binding protein from Streptomyces antibioticus (325 aa) fasta scores; opt: 611, z-score: 658.7, E(): 2.3e-29, (39.4% identity in 310 aa overlap). Also similar to TR:CAB42771 (EMBL:AL049841) from Streptomyces coelicolor (315 aa) fasta scores; opt: 599, z-score: 646.2, E(): 1.1e-28, (40.9% identity in 291 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite matches to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 2 2 5 22 [Reporter]SCO5394 (1B7)_PCR/2629 [Gene]SCO5394/2394 NC_003888 2SC6G5.38c, possible ABC transporter integral membrane subunit, len: 256aa; similar to many membrane proteins of eg. TR:O86630 (EMBL:AL031155) probable integral membrane protein from Streptomyces coelicolor (279 aa) fasta scores; opt: 504, z-score: 572.6, E(): 2e-24, 37.6% identity in 258 aa overlap.. Contains possible membrane-spanning hydrophobic regions and lies immediately downstream of a possible ABC transporter ATP-binding subunit. 2 1 11 16 [Reporter]SCO7259 (7B8)_PCR/739 [Gene]SCO7259/675 NC_003888 SC5H1.33c, hypothetical protein, len: 319 aa; unknown function, similar to TR:O33938 (EMBL:Y11199) eryBIV, Saccharopolyspora erythraea putative deoxyhexose reductase involved in mycarose biosynthesis (322 aa), fasta scores; opt: 174 z-score: 200.8 E(): 0.00073, 28.7% identity in 282 aa overlap 2 2 5 21 [Reporter]SCO0666 (2N3)_PCR/2628 [Gene]SCO0666/2393 NC_003888 SCF91.26, catB, catalase (EC 1.11.1.6), len: 759 aa. Previously sequenced and characterised from Streptomyces coelicolor strain ATCC10147 EMBL:AF000419 fasta scores opt:5163 z-score:0.0 E(): 0.0 100.00% identity in 759 aa overlap. Contains two Prosite hits to PS00437 Catalase proximal heme-ligand signature and PS00438 Catalase proximal active site signature. Also contains a Pfam match to entry PF00199 catalase, Catalase. 2 1 11 15 [Reporter]SCO7238 (8N4)_PCR/738 [Gene]SCO7238/674 NC_003888 SC7A12.05, unknown, len: 125 aa. Similar to Streptomyces coelicolor TR:Q9X8F4(EMBL:AL049573) hypothetical 14.3 kd protein, SCE39.31C (136 aa), fasta scores opt: 247 z-score: 310.9 E(): 7.6e-10 37.2% identity in 121 aa overlap. 2 2 5 20 [Reporter]SCO4714 (3J3)_PCR/2627 [Gene]SCO4714/2392 NC_003888 SCD31.39, rplE, 50S ribosomal protein L5, len: 185 aa; highly similar to SW:RL5_ECOLI (EMBL:X01563) Escherichia coli 50S ribosomal protein L5 RplE, 178 aa; fasta scores: opt: 745 z-score: 920.9 E(): 0; 58.8% identity in 177 aa overlap. Contains Pfam matches to entries PF00281 Ribosomal_L5, Ribosomal protein L5 and PF00673 Ribosomal_L5_C, ribosomal L5P family C-terminus and match to Prosite entry PS00358 Ribosomal protein L5 signature 2 1 11 14 [Reporter]SCO7815 (9J4)_PCR/737 [Gene]SCO7815/673 NC_003888 SC8E7.12 possible TetR-family transcriptional regulator, len: 223 aa. Highly similar to many putative regulators e.g. Mycobacterium tuberculosis TR:O53737 (EMBL:AL021932) putative transcriptional regulator (236 aa), fasta scores opt: 471 z-score: 574.1 E(): 1.7e-24 39.0% identity in 218 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with a putative helix-turn-helix motif situated between residues 44..65 (+4.94 SD). 2 2 5 19 [Reporter]SCO6905 (4F3)_PCR/2626 [Gene]SCO6905/2391 NC_003888 SC1B2.11, unknown, len: 125 aa. 2 1 11 13 [Reporter]SCO5127 (10F4)_PCR/736 [Gene]SCO5127/672 NC_003888 SC9E12.12, possible integral membrane protein, len: 1450 aa. Contains possible hydrophobic membrane spanning regions 2 2 5 18 [Reporter]SCO6346 (5B3)_PCR/2625 [Gene]SCO6346/2390 NC_003888 SC3A7.14, unknown, len: 158 aa 2 1 11 12 [Reporter]SCO2268 (11B4)_PCR/735 [Gene]SCO2268/671 NC_003888 SCC75A.14c, unknown, len: 214 aa. Highly similar to Mycobacterium tuberculosis SW:YR16_MYCTU (EMBL:Z96072) hypothetical 24.6 KD protein (228 aa), fasta scores: opt: 447 z-score: 555.4 E(): 1.5e-23 44.1% identity in 220 aa overlap and to the C-terminus of Mycobacterium tuberculosis SW:TYSY_MYCTU (EMBL:X59273) putative thymidylate synthase (EC 2.1.1.45) (450 aa), fasta scores opt: 447 z-score: 550.9 E(): 2.6e-23 44.1% identity in 220 aa overlap 2 1 11 11 [Reporter]SCO4442 (11N24)_PCR/734 [Gene]SCO4442/670 NC_003888 SCD6.20, hypothetical protein, len: 63 aa; similar to many other S. coelicolor hypothetical proteins, e.g. TR:CAB62714 (EMBL:AL133423) Streptomyces coelicolor hypothetical 6.9 kD protein SC4A7.10, 65 aa; fasta scores: opt: 159 z-score: 242.8 E(): 4.6e-06; 50.9% identity in 55 aa overlap 3 4 16 22 [Reporter]SCO1204 (1O13)_PCR/7406 [Gene]SCO1204/6749 NC_003888 2SCG58.04, probable aldehyde dehydrogenase, len: 461 aa; similar to SW:GABD_ECOLI (EMBL:M88334) Escherichia coli succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.16) GabD, 482 aa; fasta scores: opt: 1006 z-score: 1146.9 E(): 0; 36.5% identity in 455 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family 3 4 16 21 [Reporter]SCO4725 (2K13)_PCR/7405 [Gene]SCO4725/6748 NC_003888 SC6G4.03, infA, translational initiation factor IF1, len: 73 aa; identical to TR:O54209 (EMBL:X83011) translational initiation factor IF1 (fragment) (65 aa) and highly similar to many e.g. IF1_BACSU translation initiation factor IF-1 (71 aa), fasta scores; opt: 384 z-score: 792.5 E(): 0 , 78.6% identity in 70 aa overlap. Contains Pfam match to e ntry PF00575 S1, S1 RNA binding motif, score 54.10, E-value 3.1e-12 3 4 16 20 [Reporter]SCO2215 (3G13)_PCR/7404 [Gene]SCO2215/6747 NC_003888 SC10B7.10, possible two-component system sensor kinase, len: 425 aa; similar to various sensor kinases, e.g. TR:Q9ZBS5 (EMBL:AL034446) Streptomyces coelicolor SC1A9.27c, 303 aa; fasta scores: opt: 304 z-score: 337.8 E(): 2e-11; 29.1% identity in 306 aa overlap and low similarity to C-terminal region of SW:NARX_ECOLI (EMBL:X13360) Escherichia coli mitrate/nitrite sensor protein (EC 2.7.3.-) NarX, 598 aa; fasta scores: opt: 214 z-score: 235.8 E(): 9.4e-06; 27.8% identity in 194 aa overlap. Contains possible hydrophobic membrane spanning regions 3 4 16 19 [Reporter]SCO5474 (4C13)_PCR/7403 [Gene]SCO5474/6746 NC_003888 SC2A11.08, unknown, len: 255 aa 3 4 16 18 [Reporter]SCO2088 (5O9)_PCR/7402 [Gene]SCO2088/6745 NC_003888 SC4A10.21c, murF, probable UDP-N-acetylmuramoylalanyl-D-glutamyl- 2,6-diaminopimelate- D-alanyl-alanyl ligase, len: 471 aa; similar to many e.g. SW:MURF_ECOLI (EMBL:X15432), Mra, MurF, Escherichia coli UDP-N-acetylmuramoylalanyl-D-glutamyl- 2,6-diaminopimelate- D-alanyl-alanyl ligase (452 aa), fasta scores; opt: 774 z-score: 866.3 E(): 0, 36.1% identity in 471 aa overlap. Also similar to SW:MURF_STRTO (EMBL:U82965), MurF, MurX, Streptomyces toyocaensis probable UDP-N-acetylmuramoylalanyl-D-glutamyl- 2,6-diaminopimelate- D-alanyl-alanyl ligase (443 aa) (50.4% identity in 464 aa overlap). Shows weak similarity to others from S.coelicolor e.g. SC9B1.07 (EMBL:AL049727) S.coelicolor possible UDP-N-acetylmuramoyl-L-alanine ligase (462 aa) (24.1% identity in 464 aa overlap). Contains Pfam match to entry PF01225 Mur_ligase, Mur ligase family 3 4 16 17 [Reporter]SCO7456 (6K9)_PCR/7401 [Gene]SCO7456/6744 NC_003888 SC5C11.13c, unknown, len: 163 aa. 3 4 16 16 [Reporter]SCO5682 (7G9)_PCR/7400 [Gene]SCO5682/6743 NC_003888 SC5H4.06c, possible integral membrane protein, len: 377 aa; similar to TR:Q53873 (EMBL:AL031317) Streptomyces coelicolor putative integral membrane protein SC6G4.11, 411 aa; fasta scores: opt: 180 z-score: 193.2 E(): 0.0027; 33.8% identity in 340 aa overlap. Contains possible hydrophobic membrane spanning regions 3 4 16 14 [Reporter]SCO7829 (9O5)_PCR/7398 [Gene]SCO7829/6742 NC_003888 SC8E7.26, unknown, len: 288 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1X5(EMBL:AL109973) hypothetical 31.6 kd protein, SCJ30.14C (288 aa). Also highly similar in regions to another Streptomyces coelicolor protein of undefined function: TR:Q9S1T4(EMBL:AL109950) SCJ4.39 (240 aa), fasta scores opt: 334 z-score: 398.6 E(): 1e-14 41.6% identity in 245 aa overlap. 2 3 22 12 [Reporter]SCO4333 (11N10)_PCR/5009 [Gene]SCO4333/4569 NC_003888 SCD12A.16c, possible integral membrane protein, len: 86 aa. Contains possible hydrophobic membrane spanning regions 3 4 16 13 [Reporter]SCO2788 (10K5)_PCR/7397 [Gene]SCO2788/6741 NC_003888 SCC105.19, unknown, len: 85 aa 2 3 22 11 [Reporter]SCO4148 (12J10)_PCR/5008 [Gene]SCO4148/4568 NC_003888 SCD84.15, probable ABC transport system ATP-binding protein, len: 289 aa; similar to TR:AAF23811 (EMBL:AF213822) Zymomonas mobilis ABC binding protein, 298 aa; fasta scores: opt: 607 z-score: 650.5 E(): 8.9e-29; 38.6% identity in 285 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 4 16 12 [Reporter]SCO2281 (11G5)_PCR/7396 [Gene]SCO2281/6740 NC_003888 SCC75A.27c, possible two-component system response-regulator, len: 228 aa. Similar to many other regulators including: Rhodococcus sp TR:O85372(EMBL:AF064700) two-component regulator CprR (212 aa), fasta scores opt: 295 z-score: 351.2 E(): 3.5e-12 31.5% identity in 203 aa overlap and Streptomyces coelicolor TR:CAB50960(EMBL:AL096849) putative two-component system response regulator SCI11.34 (221 aa), fasta scores opt: 336 z-score: 398.1 E(): 8.4e-15 34.4% identity in 224 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family. 2 3 22 10 [Reporter]SCO3251 (13F10)_PCR/5007 [Gene]SCO3251/4567 NC_003888 SCE39.01c, partial CDS, probable excisionase, len: >33 aa; similar to others eg. TR:E1286473 (EMBL:AJ005260) Xis, excisionase fron the integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens (62 aa) fasta scores; opt: 143, z-score: 280.9, E(): 2.4e-08, (96.0% identity in 25 aa overlap).,SCE29.20c, probable excisionase, len: 66 aa; highly similar to SW:VXIS_STRAM excisionase from the integrated conjugative pSAM plasmid of Streptomyces ambofaciens (62 aa) fasta scores; opt: 290, z-score: 444.1, E(): 2e-17, (80.7% identity in 57 aa overlap). 2 3 22 9 [Reporter]SCO3414 (14B10)_PCR/5006 [Gene]SCO3414/4566 NC_003888 SCE9.21c, possible transcriptional regulator, len: 148 aa; weakly similar to TR:P71885 (EMBL:Z79702) Mycobacterium tuberculosis hypothetical protein (163 aa), fasta scores; opt: 166 z-score: 220.3 E(): 6e-05, 29.5% identity in 139 aa overlap and TR:O34949 (EMBL:AJ002571), ykoM, Bacillus subtilis hypothetical protein (154 aa) (27.0% identity in 141 aa overlap). Contains Pfam match to entry PF01047 MarR, MarR family, score 71.50, E-value 1.8e-17 2 3 22 8 [Reporter]SCO5162 (15N6)_PCR/5005 [Gene]SCO5162/4565 NC_003888 SCP8.25, possible integral membrane protein, len: 201 aa; similar to SW:YCHE_ECOLI (EMBL:AE000222) Escherichia coli hypothetical 23.5 kD protein in AdhE-OppA intergenic region YchE, 215 aa; fasta scores: opt: 406 z-score: 496.2 E(): 3.6e-20; 30.0% identity in 207 aa overlap. Contains Pfam match to entry PF01914 UPF0056, Uncharacterized protein family UPF0056 and possible hydrophobic membrane spanning regions 2 3 22 7 [Reporter]SCO0852 (16J6)_PCR/5004 [Gene]SCO0852/4564 NC_003888 SCM2.05c, possible aldolase, len: 210 aa. Similar to Bacillus subtilis SW:ALKH_BACSU (EMBL:L47838) 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14) (196 aa), fasta scores opt: 383 z-score: 406.3 E(): 3e-15 38.5% identity in 179 aa overlap. Contains a Pfam match to entry PF01081 Aldolase, KDPG and KHG aldolase. 2 3 22 6 [Reporter]SCO1543 (17F6)_PCR/5003 [Gene]SCO1543/4563 NC_003888 SCL2.33c, putative membrane protein, len: 153 aa. Contains possible hydrophobic membrane spanning regions 2 2 5 17 [Reporter]SCO7070 (5N23)_PCR/2624 [Gene]SCO7070/2389 NC_003888 SC4G1.36, unknown, len: 388 aa. High content in alanine, arginine, glycine and proline amino acid residues. Also contains TTA leucine codon, possible target for bldA regulation 2 3 22 5 [Reporter]SCO1772 (18B6)_PCR/5002 [Gene]SCO1772/4562 NC_003888 SCI51.12c, possible partitioning or sporulation protein, len: 340 aa; the C-terminal region is similar to SW:SOJ_BACSU (EMBL:D26185), Soj, Bacillus subtilis sporulation protein (253 aa), fasta scores; opt: 790 z-score: 857.6 E(): 0, 48.8% identity in 250 aa overlap and TR:O05189 (EMBL:U87804), ParA, Caulobacter crescentus chromosome partitioning protein (266 aa) (46.3% identity in 255 aa overlap). It is closer in predicted length to similar proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. TR:O33207 (EMBL:Z98268) Mycobacterium tuberculosis hypothetical protein (318 aa) (65.8% identity in 301 aa overlap). Similar to SCH24.08 (EMBL:AL049826) S.coelicolor possible partitioning or sporulation protein (275 aa) (45.8% identity in 249 aa overlap) to many other members of the parA family. The start codon position is suggested by GC frameplot and codon usage plots. Alternative start codons are present at codons 3, 8 and 11. Contains Pfam match to entry PF00991 ParA, ParA family ATPase 2 2 5 16 [Reporter]SCO4843 (6J23)_PCR/2623 [Gene]SCO4843/2388 NC_003888 SC5G8.11, possible integral membrane protein, len: 169aa; similar to many of undefined function eg. TR:O53584 (EMBL:AL022076) hypothetical protein from Mycobacterium tuberculosis (165 aa) fasta scores; opt: 324, z-score: 386.8, E(): 4.3e-14, 41.5% identity in 159 aa overlap. Contains Pfam match to entry PF01569 PAP2, PAP2 superfamily and possible membrane-spanning hydrophobic regions. 2 3 22 4 [Reporter]SCP1.289 (19N2)_PCR/5001 [Gene]SCP1.289/4561 NC_003888 SCP1.289, unknown, len: 91aa; 2 1 11 10 [Reporter]SCO2842 (12J24)_PCR/733 [Gene]SCO2842/669 NC_003888 SCE20.16c, possible membrane protein,len: 184 aa. Contains possible membrane spanning hydrophobic domain and a possible N-terminal signal sequence. 2 2 5 15 [Reporter]SCO7441 (7F23)_PCR/2622 [Gene]SCO7441/2387 NC_003888 SC6D11.37, possible transcriptional regulatory protein, len: 197 aa. Similar to several putative regulators e.g. Streptomyces lividans TR:O85695(EMBL:AF072709) putative transcriptional regulator (192 aa), fasta scores opt: 170 z-score: 212.6 E(): 0.0002 29.7% identity in 192 aa overlap. Contains a possible helix-turn-helix motif situated between residues 29..50 (+3.58 SD). 2 3 22 3 [Reporter]SCP1.191 (20J2)_PCR/5000 [Gene]SCP1.191/4560 NC_003888 SCP1.191, unknown, len: 59aa; 2 1 11 9 [Reporter]SCO1581 (13F24)_PCR/732 [Gene]SCO1581/668 NC_003888 SCI35.03, putative membrane protein, len: 187 aa. Contains possible hydrophobic membrane spanning regions 2 2 5 14 [Reporter]SCO5940 (8B23)_PCR/2621 [Gene]SCO5940/2386 NC_003888 SC7H1.10, probable transferase, len: 219 aa; similar to eg. MENG_ECOLI P32165 s-adenosylmethionine:2-demethylmenaquinone methyltransferase (161 aa), fasta scores; opt: 211 z-score: 265.9 E(): 1.3e-07, 34.1% identity in 126 aa overlap 2 1 11 8 [Reporter]SCO0520 (14B24)_PCR/731 [Gene]SCO0520/667 NC_003888 SCF6.16, putative tetR family transcriptional regulator, len: 194 aa. Weakly similar to many other tetR family regulators e.g. Streptomyces sp. TR:O24741 (EMBL:AB001683) FarA (221 aa), fasta scores opt: 161 z-score: 205.5 E(): 0.00044 28.4% identity in 190 aa overlap and Streptomyces coelicolor TR:O86852 (EMBL:AJ007731) gamma-butyrolactone binding protein (215 aa), fasta scores opt: 149 z-score: 191.2 E(): 0.0028 26.6% identity in 188 aa overlap. Also highly similar to Streptomyces coelicolor SCF51A.08 (EMBL; AL121596) putative tetR family transcriptional regulator (227 aa), fasta scores opt: 479 z-score: 463.7 E(): 1.5e-20 43.7% identity in 183 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. The possible helix-turn-helix motif lies between residues 36..57 (+4.21 SD). 2 2 5 13 [Reporter]SCO6449 (9N19)_PCR/2620 [Gene]SCO6449/2385 NC_003888 SC9B5.16, possible dehydrogenase, len: 472 aa; similar to many, almost identical to SW:P49_STRLI P49 protein from Streptomyces lividans (469 aa) fasta scores; opt: 3162, z-score: 3290.5, E(): 0, (99.1% identity in 469 aa overlap). Also similar to SW:CRTI_RHOCA phytoene dehydrogenase from Rhodobacter capsulatus (524 aa) fasta scores; opt: 169, z-score: 305.6, E(): 9.8e-10, (25.0% identity in 509 aa overlap). Contains possible hydrophobic membrane spanning region 2 1 11 7 [Reporter]SCO0072 (15N20)_PCR/730 [Gene]SCO0072/666 NC_003888 SCJ4.53c, partial CDS, unknown, len: >356 aa; similar to many of undefined function e.g. SW:WAPA_BACSU wall associated protein from Bacillus subtilis (2334 aa) fasta scores; opt: 322, z-score: 315.0, E(): 3.5e-10, (27.5% identity in 320 aa overlap).,SCJ11.01c, partial CDS, possible secreted protein, len: >793 aa 2 2 5 12 [Reporter]SCO2731 (10J19)_PCR/2619 [Gene]SCO2731/2384 NC_003888 SCC57A.02, probable cation transporting P-type ATPase, len: 760 aa. Highly similar to many putative cation transporters e.g. Mycobacterium leprae SW:CTPB_MYCLE(EMBL:Z46257) cation-transporting P-type ATPase B (EC 3.6.1.-) (750 aa), fasta scores opt: 1955 z-score: 1929.1 E():0 49.5% identity in 750 aa overlap and Streptomyces coelicolor (EMBL:AL136149) SCM2.13c (762 aa), fasta scores opt: 2552 z-score: 2109.2 E():0 55.8% identity in 749 aa overlap. Contains Prosite hits to PS00154 E1-E2 ATPases phosphorylation site, PS01229 Hypothetical cof family signature 2 and PS01047 Heavy-metal-associated domain. Also contains Pfam hits to PF00122 E1-E2_ATPase, E1-E2 ATPase and PF00403 HMA, Heavy-metal-associated domain, as well as, several possible membrane spanning hydrophobic domains. 2 1 11 6 [Reporter]SCO0802 (16J20)_PCR/729 [Gene]SCO0802/665 NC_003888 SCF43.13c, unknown, len: 282 aa. Highly similar to several including: Bacillus megaterium TR:O24772(EMBL:AB005787) oxetanocin A resistance protein, OxrA (185 aa), fasta scores opt: 632 z-score: 753.8 E():0 56.8% identity in 176 aa overlap and Bacillus subtilis SW:YYBG_BACSU(EMBL:D26185) hypothetical 31.3 KD protein (279 aa), fasta scores opt: 1070 z-score: 1267.0 E():0 59.0% identity in 266 aa overlap. Contains 2xPfam matches to entry PF00805 Pentapeptide, Pentapeptide repeats (8 copies) 2 2 5 11 [Reporter]SCO4589 (11F19)_PCR/2618 [Gene]SCO4589/2383 NC_003888 SCD20.07c, probable aminopeptidase (putative secreted protein), len: 324 aa; similar to SW:APX_STRGR Streptomyces griseus aminopeptidase (EC 3.4.11.-) SgaP, 284 aa; fasta scores: opt: 1532 z-score: 1626.6 E(): 0; 76.8% identity in 276 aa overlap and to (EMBL:AL391754) Streptomyces coelicolor putative hydrolase SCK7.16c, 486 aa; fasta scores: opt: 980 z-score: 845.8 E(): 0; 52.1% identity in 288 aa overlap. Contains possible N-terminal region signal peptide sequence 2 1 11 5 [Reporter]SCO3836 (17F20)_PCR/728 [Gene]SCO3836/664 NC_003888 SCH69.06c, possible rRNA methylase, len: 261aa; similar to many eg. TR:O84408 (EMBL:AE001313) rRNA methylase (269 aa) fasta scores; opt: 197 z-score: 225.0 E(): 3.3e-05 (26.8% identity in 235 aa overlap). Contains Pfam match to entry PF00588 SpoU_methylase, SpoU rRNA Methylase family. 2 2 5 10 [Reporter]SCO4292 (12B19)_PCR/2617 [Gene]SCO4292/2382 NC_003888 SCD95A.25c, hypothetical protein, len: 314 aa; similar to TR:Q49955 (EMBL:U15180) Mycobacterium leprae U1756L, 318 aa; fasta scores: opt: 792 z-score: 901.0 E(): 0; 48.0% identity in 304 aa overlap 2 1 11 4 [Reporter]SCO1827 (18B20)_PCR/727 [Gene]SCO1827/663 NC_003888 SCI8.12, possible DNA polymerase III, epsilon chain (EC 2.7.7.7), len: 328 aa. Similar to many including: Mycobacterium tuberculosis TR:O69678 (EMBL: AL022121) hypothetical 35.8 KD protein (329 aa), fasta scores opt: 509 z-score: 584.3 E(): 3.6e-25 41.6% identity in 315 aa overlap and Escherichia coli SW: DP3E_ECOLI (EMBL: X04027) DNA polymerase III, epsilon chain (EC 2.7.7.7) (243 aa), fasta scores opt: 251 z-score: 293.6 E(): 5.5e-09 27.9% identity in 204 aa overlap. Contains a Pfam match to entry PF00929 Exonuclease, Exonuclease. 2 2 5 9 [Reporter]SCO4966 (13N15)_PCR/2616 [Gene]SCO4966/2381 NC_003888 2SCK31.26c, possible membrane protein, len: 132 aa; similar to TR:O53429 (EMBL:AL021897) Mycobacterium tuberculosis hypothetical 15.4 kDa protein MTV017.34c, 144 aa; fasta scores: opt: 186 z-score: 240.5 E(): 7.4e-06; 27.9% identity in 136 aa overlap. Contains possible hydrophobic membrane spanning region 2 1 11 3 [Reporter]SCP1.118 (19N16)_PCR/726 [Gene]SCP1.118/662 NC_003888 SCP1.118, doubtful CDS, unknown, len: 59aa; 2 2 5 8 [Reporter]SCO1833 (14J15)_PCR/2615 [Gene]SCO1833/2380 NC_003888 SCI8.18c, possible membrane protein, len: 226 aa. Contains a possible N-terminal signal sequence and a possible membrane spanning hydrophobic domain. 2 1 11 2 [Reporter]SCO3978 (20F16)_PCR/725 [Gene]SCO3978/661 NC_003888 SCBAC25E3.15c, possible oxidoreductase, len: 294aa: similar to many eg. TR:Q53929 (EMBL:X62373) ORF 4 from the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor (306 aa) fasta scores; opt: 624, Z-score: 633.9, 40.199% identity (41.581% ungapped) in 301 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and Prosite match to PS01162 Quinone oxidoreductase / zeta-crystallin signature. 2 1 10 22 [Reporter]SCO2808 (1F12)_PCR/723 [Gene]SCO2808/660 NC_003888 2SCC13.16, possible secreted protein, len: 342 aa. Contains possible N-terminal region signal peptide sequence 3 4 16 11 [Reporter]SCO4794 (12C5)_PCR/7395 [Gene]SCO4794/6739 NC_003888 SCD63A.05, possible integral membrane protein, len: 178 aa. Contains possible hydrophobic membrane spanning regions and a TTA leucine codon (codon 57), possible target for bldA regulation 3 4 16 10 [Reporter]SCO3026 (13O1)_PCR/7394 [Gene]SCO3026/6738 NC_003888 SCE34.07c, hypothetical protein, len: 375 aa; similar to TR:O05899 (EMBL:Z95121) Mycobacterium tuberculosis hypothetical 35.3 kD protein RV3256c, 346 aa; fasta scores: opt: 171 z-score: 187.3 E(): 0.0057; 31.1% identity in 376 aa overlap 3 4 16 9 [Reporter]SCO0609 (14K1)_PCR/7393 [Gene]SCO0609/6737 NC_003888 StF55.33, possible integral membrane protein, len: 287 aa; similar to TR:CAB46783 (EMBL:AL096811) Streptomyces coelicolor putative integral membrane protein, 297 aa; fasta scores: opt: 400 z-score: 446.2 E(): 1.8e-17; 34.2% identity in 278 aa overlap 3 4 16 8 [Reporter]SCO0671 (15G1)_PCR/7392 [Gene]SCO0671/6736 NC_003888 SCF91.31c, unknown, len: 84 aa. 3 4 16 7 [Reporter]SCO0513 (16C1)_PCR/7391 [Gene]SCO0513/6735 NC_003888 SCF6.09, integral membrane protein, len: 363 aa. Similar to a number of hypothetical integral membrane proteins e.g. Escherichia coli SW:PERM_ECOLI (EMBL; AE000335) putative permease PerM (353 aa), fasta scores opt: 383 z-score: 425.2 E(): 2.6e-16 27.5% identity in 346 aa overlap and Mycobacterium tuberculosis TR:O53656 (EMBL; AL021928) hypothetical 38.0 KD protein (367 aa), fasta scores opt: 687 z-score: 755.9 E():0 33.5% identity in 340 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a Pfam match to entry PF01594 DUF20, Putative permease. 3 4 16 6 [Reporter]SCO3839 (16O21)_PCR/7390 [Gene]SCO3839/6734 NC_003888 SCH69.09c, unknown, len: 109aa; similar to SW:GLPE_ECOLI protein of unknown function from the sn-glycerol 3-phosphate regulon of Escherichia coli (108 aa) fasta scores; opt: 154, z-score: 209.1, E(): 0.00026, (33.3% identity in 78 aa overlap). 3 4 16 5 [Reporter]SCO1608 (17K21)_PCR/7389 [Gene]SCO1608/6733 NC_003888 SCI35.30c, unknown, len: 237 aa; start uncertain, may be considerably further upstream 3 4 16 4 [Reporter]SCO0654 (18G21)_PCR/7388 [Gene]SCO0654/6732 NC_003888 SCF91.14, unknown, len: 324 aa. Highly similar to several proteins of undefined function from Streptomyces coelicolor including TR:CAB44391 (EMBL:AL078610) SCH35.36C (320 aa), fasta scores opt: 1125 z-score: 985.2 E(): 0 57.4% identity in 305 aa overlap and TR:Q9ZC09 (EMBL:AL033505) SC1E6.13 (3376 aa), fasta scores opt: 934 z-score: 819.6 E():0 50.4% identity in 337 aa overlap. Contains a probable coiled-coil from residue 249 to 287 (39 residues) 2 3 21 22 [Reporter]SCO1144 (1B22)_PCR/4997 [Gene]SCO1144/4559 NC_003888 2SCG38.37c, possible ABC transporter ATP-binding protein, len: 600 aa; similar to TR:P71082 (EMBL:Z82044) Bacillus subtilis unidentified transporter-ATP binding YgaD, 589 aa; fasta scores: opt: 1229 z-score: 1240.6 E(): 0; 34.2% identity in 576 aa overlap. Contains Pfam matches to entries PF00664 ABC_membrane, ABC transporter transmembrane region and PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also contains possible hydrophobic membrane spanning regions 3 4 16 3 [Reporter]SCO6090 (19C21)_PCR/7387 [Gene]SCO6090/6731 NC_003888 SCBAC1A6.14, antibiotic resistance macrolide glycosyltransferase, len: 418 aa; identical to SW:MGT_STRLI (EMBL:M74717) Streptomyces lividans macrolide glycosyltransferase (EC 2.4.1.*) Mgt, 418 aa. Contains Pfam match to entry PF00201 UDPGT, UDP-glucoronosyl and UDP-glucosyl transferases and match to Prosite entry PS00375 UDP-glycosyltransferases signature 2 3 21 21 [Reporter]SCO5007 (2N18)_PCR/4996 [Gene]SCO5007/4558 NC_003888 SCK15.09, minD2, possible septum site-determining protein, len: 131 aa; similar to C-terminal domain of TR:Q9ZF15 (EMBL:AJ005576) Streptomyces griseus septum site-determining protein MinD protein, 396 aa; fasta scores: opt: 360 z-score: 444.6 E(): 2.7e-17; 47.3% identity in 129 aa overlap and to Streptomyces coelicolor septum site-determining protein minD3 SCK15.10, 131 aa; fasta scores: opt: 177 z-score: 196.8 E(): 2.1e-05; 35.8% identity in 109 aa overlap. Contains possible hydrophobic membrane spanning region and TTA leucine codon, possible target for bldA regulation 3 4 16 2 [Reporter]SCO2826 (20K17)_PCR/7386 [Gene]SCO2826/6730 NC_003888 SCBAC17F8.17, hypothetical protein, len: 157 aa: no significant database matches. 2 3 21 20 [Reporter]SCO6420 (3J18)_PCR/4995 [Gene]SCO6420/4557 NC_003888 SC1A6.09c, unknown, len: 337 aa; similar to M. tuberculosis hypothetical protein TR:O06218 (EMBL:O06218) MTCY270.09 (344 aa), fasta scores; opt: 632 z-score: 560.4 E(): 4.3e-24, 38.1% identity in 341 aa overlap. Contains possible helix-turn-helix motif at aa 23-44 (Score 987, +2.55 SD) 2 3 21 19 [Reporter]SCO5608 (4F18)_PCR/4994 [Gene]SCO5608/4556 NC_003888 SC2E1.25, unknown prophage gene, len: 233 aa. Contains possible hydrophobic membrane spanning regions 2 3 21 18 [Reporter]SCO2412 (5B18)_PCR/4993 [Gene]SCO2412/4555 NC_003888 SC4A7.40c, possible membrane protein, len: 219 aa; similar to C-terminus of TR:P71415 (EMBL:U75438) Halobacterium salinarium transducer Htd protein, 777 aa; fasta scores: opt: 182 z-score: 198.8 E(): 0.0011; 30.6% identity in 193 aa overlap and to S. coelicolor SC4A7.38c, 253 aa; fasta scores: opt: 323 z-score: 286.8 E(): 1.4e-10; 40.5% identity in 200 aa overlap 2 3 21 17 [Reporter]SCO6751 (6N14)_PCR/4992 [Gene]SCO6751/4554 NC_003888 SC5F2A.34c, possible efflux protein, len: 312aa; similar to many eg. SW:CZCD_ALCSP cation efflux protein from Alcaligenes sp. (316 aa) fasta scores; opt: 834, z-score: 949.4, E(): 0, (47.0% identity in 300 aa overlap). Contains several probable membrane spanning hydrophobic regions. 2 3 21 16 [Reporter]SCO1417 (7J14)_PCR/4991 [Gene]SCO1417/4553 NC_003888 SC6D7.22, possible GntR-family regulatory protein, len: 500 aa. Similar to several including: Streptomyces coelicolor TR:O88047 (EMBL:AL031541) putative transcriptional regulator SCI35.09C (480 aa), fasta scores opt: 403 z-score: 439.9 E(): 4e-17 29.0% identity in 483 aa overlap and Streptomyces venezuelae TR:AAF01064 (EMBL:AF189258) transcriptional regulator (fragment) (507 aa), fasta scores opt: 444 z-score: 484.0 E(): 1.4e-19 31.8% identity in 462 aa overlap. Contains a Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and a Prosite hit to PS00043 Bacterial regulatory proteins, gntR family signature. 2 2 5 7 [Reporter]SCO1172 (15F15)_PCR/2614 [Gene]SCO1172/2379 NC_003888 SCG11A.03, probable amidase (putative secreted protein), len: 201 aa; similar to SW:AMPD_ECOLI (EMBL:X15237) Escherichia coli anhydro-N-acetylmuramyl-tripeptide amidase AmpD, 183aa; fasta scores: opt: 211 z-score: 267.1 E(): 1.7e-07; 39.5% identity in 114 aa overlap and to Streptomyces coelicolor SC2A11.21c; fasta scores: opt: 674 z-score: 751.6 E(): 0; 50.0% identity in 202 aa overlap. Contains match to Pfam entry PF01510 Amidase_2, N-acetylmuramoyl-L-alanine amidase. Contains possible N-terminal region signal peptide sequence 2 3 21 15 [Reporter]SCO5221 (8F14)_PCR/4990 [Gene]SCO5221/4552 NC_003888 SC7E4.18, probable polypeptide deformylase, len: 216 aa; similar to SW:DEF_FREDI (EMBL:Y10305) Fremyella diplosiphon polypeptide deformylase (EC 3.5.1.31) Def, 187 aa; fasta scores: opt: 353 z-score: 430.0 E(): 1.8e-16; 33.8% identity in 145 aa overlap and to TR:Q9XAQ2 (EMBL:AL078618) Streptomyces coelicolor putative polypeptide deformylase SC10A7.23, 208 aa; fasta scores: opt: 680 z-score: 796.7 E(): 0; 59.8% identity in 169 aa overlap. Contains Pfam match to entry PF01327 Pep_deformylase, Polypeptide deformylase 2 2 5 6 [Reporter]SCO5056 (16B15)_PCR/2613 [Gene]SCO5056/2378 NC_003888 SCK7.29c, possible exoribonuclease large subunit, len: 402 aa; similar to TR:O53456 (EMBL:AL021897) Mycobacterium tuberculosis exonuclease VII large subunit XseA or MTV017.61c, 415 aa; fasta scores: opt: 1318 z-score: 1486.0 E(): 0; 52.3% identity in 407 aa overlap and to SW:EX7L_ECOLI (EMBL:J02599) Escherichia coli exodeoxyribonuclease large subunit (EC 3.1.11.6) XseA, 456 aa; fasta scores: opt: 610 z-score: 689.7 E(): 6e-31; 30.8% identity in 413 aa overlap 2 3 21 14 [Reporter]SCO5420 (9B14)_PCR/4989 [Gene]SCO5420/4551 NC_003888 SC8F4.24, che, cholesterol esterase, len: 215aa; strongly similar to TR:Q53425 (EMBL:S71532) che, cholesterol esterase from Streptomyces lavendulae (227 aa) fasta scores; opt: 632, z-score: 735.9, E(): 0, 51.5% identity in 231 aa overlap. Contains possible hydrophobic membrane spanning region 2 1 10 21 [Reporter]SCO1496 (2B12)_PCR/722 [Gene]SCO1496/659 NC_003888 SC9C5.20c, aroF, chorismate synthase, len: 394 aa; similar to SW:AROC_BACSU (EMBL:M80245) Bacillus subtilis chorismate synthase (EC 4.6.1.4) AroF, 368 aa; fasta scores: opt: 1151 z-score: 1269.7 E(): 0; 48.9% identity in 358 aa overlap. Contains Pfam match to entry PF01264 Chorismate_synt, Chorismate synthase and match to Prosite entry PS00788 Chorismate synthase signature 2 and PS00789 Chorismate synthase signature 3 2 2 5 5 [Reporter]SCO0124 (17N11)_PCR/2612 [Gene]SCO0124/2377 NC_003888 SCJ21.05, unknown, len: 453 aa; similar in part to several polyketide synthases, e.g. HETM_ANASP polyketide synthase HETM (506 aa), fasta scores; opt: 252 z-score: 267.0 E(): 1.6e-07, 33.7% identity in 199 aa overlap, and TR:Q50858 (EMBL:U24657) saframycin MX1 synthetase A (2605 aa), fasta scores; opt: 244 z-score: 248.0 E(): 1.9e-06, 28.5% identity in 372 aa overlap. Contains TTA Leu codon; possible target for regulation by bldA 2 3 21 13 [Reporter]SCO2572 (10N10)_PCR/4988 [Gene]SCO2572/4550 NC_003888 SCC123.10, possible integral membrane protein, len: 428 aa. Contains a possible N-terminal signal sequence and multiple possible membrane spanning hydrophobic domains. 2 1 10 20 [Reporter]SCO1519 (3N8)_PCR/721 [Gene]SCO1519/658 NC_003888 SCL2.09c, ruvA, holliday junction DNA helicase, len: 201 aa; highly similar to SW:RUVA_MYCTU (EMBL:Z77724) Mycobacterium tuberculosis holliday junction DNA helicase RuvA, 196 aa; fasta scores: opt: 549 z-score: 615.4 E(): 6.9e-27; 47.5% identity in 204 aa overlap and to SW:RUVA_ECOLI (EMBL:X07091) Escherichia coli holliday junction DNA helicase RuvA, 203 aa; fasta scores: opt: 308 z-score: 351.0 E(): 3.7e-12; 36.6% identity in 205 aa overlap. Contains Pfam match to entry PF01330 RuvA, Bacterial DNA recombination protein, RuvA 2 2 5 4 [Reporter]SCO0261 (18J11)_PCR/2611 [Gene]SCO0261/2376 NC_003888 SCF1.03c, probable acetyltransferase, len: 175 aa; similar to many e.g. SW:MAA_ECOLI maltose O-acetyltransferase from Escherichia coli (182 aa) fasta scores; opt: 365, z-score: 432.1, E(): 1.1e-16, (43.6% identity in 140 aa overlap). Contains Pfam match to entry PF00132 hexapep, Bacterial transferase hexapeptide (four repeats) and Prosite match to PS00101 Hexapeptide-repeat containing-transferases signature 2 1 10 19 [Reporter]SCO2254 (4J8)_PCR/720 [Gene]SCO2254/657 NC_003888 SC1G2.16c.possible transmembrane efflux protein, len: 413 aa. Highly similar to many transmembrane transport proteins e.g. Streptomyces lividans SW:CMLR_STRLI(EMBL:X59968) chloramphenicol resistance protein CmlR (392 aa), fasta scores opt: 743 z-score: 769.2 E():0 35.8% identity in 377 aa overlap and Streptomyces coelicolor TR:Q9X8V1(EMBL:AL049826) putative transmembrane efflux protein SCH24.37 (404 aa), fasta scores opt: 1313 z-score: 1352.1 E(): 0 52.0% identity in 408 aa overlap. Contains a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and multiple possible membrane spanning hydrophobic domains. 2 2 5 3 [Reporter]SCO4984 (19F11)_PCR/2610 [Gene]SCO4984/2375 NC_003888 2SCK36.07c, probable aminotransferase, len: 403 aa; similar to many, e.g. TR:O86587 (EMBL:AL031514) Streptomyces coelicolor putative aminotransferase SC2H4.04c, 402 aa; fasta scores: opt: 1881 Z-score: 2193.7 bits: 414.8 E(): 1.4e-114; 65.920% identity in 402 aa overlap. Contains Pfam match to entry PF00155 aminotran_1, Aminotransferase class-I 2 1 10 18 [Reporter]SCO6387 (5F8)_PCR/719 [Gene]SCO6387/656 NC_003888 SC3C8.06, questionable ORF, len: 188 aa; similar to the C-terminal regions of various putative transposases, e.g. TR:Q9FBV8 (EMBL:AL391338) Streptomyces coelicolor putative transposase SC8E7.24c, 549 aa; fasta scores: opt: 493 Z-score: 531.0 E(): 6e-22; 46.552% identity in 174 aa overlap. Contains TTA leucine codon, possible target for action of bldA 2 2 5 2 [Reporter]SCP1.23c (20N7)_PCR/2609 [Gene]SCP1.23c/2374 NC_003888 SCP1.23c, unknown, doubtful CDS, len: 44aa; 2 1 10 17 [Reporter]SCO2057 (6B8)_PCR/718 [Gene]SCO2057/655 NC_003888 SC4G6.26, unknown, len: 246 aa; similar to TR:Q55701 (EMBL:D64000) hypothetical protein from Synechocystis sp. strain PCC6803 (214 aa) fasta scores; opt: 158, z-score: 192.8, E(): 0.0021, (32.6% identity in 224 aa overlap). Also some simliarity to the N-terminal region of many ATP-dependent proteases eg. SW:LON_ECOLI Lon protease from Escherichia coli (784 aa) fasta scores; opt: 152, z-score: 176.8, E(): 0.016, (24.6% identity in 228 aa overlap). 2 2 4 22 [Reporter]SCO4192 (1N3)_PCR/2607 [Gene]SCO4192/2373 NC_003888 2SCD46.06, unknown, len: 358aa; 2 1 10 16 [Reporter]SCO3623 (7N4)_PCR/717 [Gene]SCO3623/654 NC_003888 SCH10.01, hypothetical protein, partial CDS, len: >119 aa; probable CDS suggested by positional base preference, GC frame analysis and amino acid composition,SC66T3.34, probable gntR-family transcriptional regulator, partial CDS, len: >128 aa; similar to many e.g. (EMBL:U91412), luxZ, Photobacterium leiognathi lux operon enhancer (221 aa), fasta scores; opt: 197 z-score: 240.2 E(): 4.7e-06, 32.2% identity in 87 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 2 2 4 21 [Reporter]SCO1243 (2J3)_PCR/2606 [Gene]SCO1243/2372 NC_003888 2SCG1.18c, bioF, 8-amino-7-oxononanoate synthase, len: 375 aa; similar to SW:BIOF_BACSH (EMBL:M29291) Bacillus sphaericus 8-amino-7-oxononanoate synthase (EC 2.3.1.47) BioF, 389 aa; fasta scores: opt: 774 z-score: 785.6 E(): 0; 39.1% identity in 379 aa overlap. Contains Pfam match to entry PF00222 aminotran_2, Aminotransferases class-II and match to Prosite entry PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site 2 1 10 15 [Reporter]SCO5583 (8J4)_PCR/716 [Gene]SCO5583/653 NC_003888 SC7A1.27, amt, ammonium transporter, len: 448 aa; similar to many eg. TR:O67997 (EMBL:AF005275) (438 aa) fasta scores; opt: 1224, z-score: 1126.9, E(): 0, (47.1% identity in 414 aa overlap). Contains PS01219 Ammonium transporters signature and Pfam match to entry PF00909 Ammonium_transp, Ammonium Transporter Family, score 537.00, E-value 1.3e-157. 2 2 4 20 [Reporter]SCO0438 (3F3)_PCR/2605 [Gene]SCO0438/2371 NC_003888 SCF51A.16c, pcp, pyrrolidone-carboxylate peptidase (EC 3.4.19.3), len: 216 aa. Highly similar to many e.g. Bacillus subtilis SW:PCP_BACSU (EMBL; X66034) pyrrolidone-carboxylate peptidase (EC 3.4.19.3) (5-oxoprolyl-peptidase) (pyroglutamyl-peptidase I) (PGP-I) (pyrase)(215 aa), fasta scores opt: 741 z-score: 820.9 E(): 0 52.3% identity in 214 aa overlap. Contains a Pfam match to entry PF01470 Peptidase_C15, Pyroglutamyl peptidase and a Prosite hit to PS01334 Pyrrolidone-carboxylate peptidase cysteine active site 2 1 10 14 [Reporter]SCO7717 (9F4)_PCR/715 [Gene]SCO7717/652 NC_003888 SC8D11.08c, possible secreted protein, len: 137 aa; similar to TR:Q9Z4W4 (EMBL:AL035654) Streptomyces coelicolor hypothetical 14.7 kDa protein SCE8.13c, 142 aa; fasta scores: opt: 173 z-score: 209.1 E(): 0.00041; 38.5% identity in 143 aa overlap. Contains possible N-terminal region signal peptide sequence 2 2 4 19 [Reporter]SCO6901 (4B3)_PCR/2604 [Gene]SCO6901/2370 NC_003888 SC1B2.07, unknown, len: 288 aa. Similar to Streptomyces coelicolor TR:CAB72355(EMBL:AL138977) hypothetical 27.8 kd protein, SC7F9.02C (259 aa), fasta scores opt: 229 z-score: 247.8 E(): 2.4e-06 31.4% identity in 283 aa overlap. 2 1 10 13 [Reporter]SCO5123 (10B4)_PCR/714 [Gene]SCO5123/651 NC_003888 SC9E12.08, possible small membrane protein, len: 64 aa; similar to TR:Q9RKX4 (EMBL:AL133213) Streptomyces coelicolor putative secreted protein SC6D7.24c, 64 aa; fasta scores: opt: 225 z-score: 329.1 E(): 7.3e-11; 56.5% identity in 62 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 10 12 [Reporter]SCO2695 (10N24)_PCR/713 [Gene]SCO2695/650 NC_003888 SCC61A.16, unknown, len: 256 aa; region similar to others eg. TR:O69671 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (425 aa) fasta scores; opt: 266, z-score: 305.4, E(): 1.5e-09, 32.3% identity in 248 aa overlap. 3 4 15 22 [Reporter]SCO1106 (1K13)_PCR/7384 [Gene]SCO1106/6729 NC_003888 2SCG4.22, possible lipoprotein, len: 218 aa; similar to C-terminal region of TR:Q9RVD0 (EMBL:AE001960) Deinococcus radiodurans conserved hypothetical protein DR1099, 197 aa; fasta scores: opt: 263 z-score: 286.8 E(): 1.7e-08; 35.4% identity in 147 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 3 4 15 21 [Reporter]SCO5044 (2G13)_PCR/7383 [Gene]SCO5044/6728 NC_003888 SCK7.17c, fumB, fumarate hydratase class I, len: 558 aa; similar to SW:FUMB_ECOLI (EMBL:M27058) Escherichia coli fumarate hydratase class I, anaerobic (EC 4.2.1.2) FumB, 548 aa; fasta scores: opt: 2386 z-score: 2782.7 E(): 0; 64.9% identity in 539 aa overlap. Contains Pfam match to entry PF00206 lyase_1, Lyase and match to Prosite entry PS00163 Fumarate lyases signature 3 4 15 20 [Reporter]SCO2209 (3C13)_PCR/7382 [Gene]SCO2209/6727 NC_003888 S10B7.04 probable transcriptional regulator, len: 174 aa; identical to previously sequenced TR:Q9X957 (EMBL:Y13833) Streptomyces coelicolor hypothetical 19.0 kD protein, 174 aa and similar to TR:Q9XA85 (EMBL:AL096837) Streptomyces coelicolor putative marR-family transcriptional regulator, SCF43A.30c, 168 aa; fasta scores: opt: 276 z-score: 336.5 E(): 2.3e-11; 39.9% identity in 153 aa overlap 3 4 15 19 [Reporter]SCO6942 (4O9)_PCR/7381 [Gene]SCO6942/6726 NC_003888 SC1G8.14c, cvnB8, unknown, len: 171 aa. Similar to many proteins of undefined function from Streptomyces coelicolor including: TR:CAB61281(EMBL:AL132991) SCF55.11C (152 aa), fasta scores opt: 158 z-score: 212.6 E(): 0.00022 33.1% identity in 160 aa overlap and TR:O86521(EMBL:AL031124) SC1C2.20C (140 aa), fasta scores opt: 177 z-score: 237.3 E(): 9.4e-06 29.4% identity in 143 aa overlap. 3 4 15 18 [Reporter]SCO2083 (5K9)_PCR/7380 [Gene]SCO2083/6725 NC_003888 SC4A10.16c, ftsQ, sporulation protein, len: 264 aa; previously sequenced as SW:FTSQ_STRCO (EMBL:U10879), ftsQ, Streptomyces coelicolor protein required for efficient sporulation, but not growth and viability (264 aa) and identical that sequence. Contains a hydrophobic, possible membrane-spanning region 3 4 15 17 [Reporter]SCO5782 (6G9)_PCR/7379 [Gene]SCO5782/6724 NC_003888 SC4H2.03c, possible transmembrane transport protein, len: 411 aa; similar to several hypothetical proteins e.g. M. tuberculosis TR:O05881 (EMBL:Z95121) MTCY20B11.11C (385 aa), fasta scores opt: 1226 z-score: 1612.0 E(): 0, 53.5% identity in 372 aa overlap, and to members of the KEFB/KEFC potassium transporters family e.g. KEFB_ECOLI P45522 glutathione-regulated potassium-efflux protein (601 aa), fastascores; opt: 327 z-score: 292.9 E(): 4.3e-09, 25.3% identity in 396 aa overlap 3 4 15 16 [Reporter]SCO5678 (7C9)_PCR/7378 [Gene]SCO5678/6723 NC_003888 SC5H4.02, possible regulatory protein, len: 552 aa; similar to TR:Q9ZBP0 (EMBL:AL034492) Streptomyces coelicolor putative regulatory protein SC6C5.05c, 569 aa; fasta scores: opt: 310 z-score: 323.3 E(): 1.5e-10; 30.0% identity in 587 aa overlap 3 4 15 15 [Reporter]SCO7248 (8O5)_PCR/7377 [Gene]SCO7248/6722 NC_003888 SC7A12.15, unknown, len: 206 aa. Weakly similar to several proteins of undefined function e.g. Streptomyces coelicolor TR:CAB72201(EMBL:AL138851) SCE59.14C (196 aa), fasta scores opt: 209 z-score: 240.8 E(): 6.1e-06 33.7% identity in 193 aa overlap. 2 3 21 12 [Reporter]SCO4329 (11J10)_PCR/4987 [Gene]SCO4329/4549 NC_003888 SCD12A.12c, possible integral membrane protein, len: 463 aa; similar to TR:Q9X8T4 (EMBL:AL049826) Streptomyces coelicolor putative membrane protein SCH24.20c, 425 aa; fasta scores: opt: 305 z-score: 344.1 E(): 1.1e-11; 28.7% identity in 411 aa overlap. Contains possible hidrophobic membrane spanning regions 3 4 15 14 [Reporter]SCO7824 (9K5)_PCR/7376 [Gene]SCO7824/6721 NC_003888 SC8E7.21c, possible TetR-family transcriptional regulator, len: 190 aa. Similar to Deinococcus radiodurans TR:Q9RWE9 (EMBL:AE001928) transcriptional regulator, TetR family (215 aa), fasta scores opt:245 z-score: 307.6 E(): 1.2e-09 33.3% identity in 168 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 32..53 (+4.08 SD) 2 3 21 11 [Reporter]SCO4169 (12F10)_PCR/4986 [Gene]SCO4169/4548 NC_003888 SCD66.06, probable oxidoreductase, len: 254 aa; similar to SW:2BHD_STREX Streptomyces exfoliatus 20-beta-hydroxysteroid dehydrogenase (EC 1.1.1.53), 255 aa; fasta scores: opt: 1285 z-score: 1410.5 E(): 0; 77.6% identity in 255 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 3 4 15 13 [Reporter]SCO5137 (10G5)_PCR/7375 [Gene]SCO5137/6720 NC_003888 SC9E12.22c, possible ATP-binding protein, len: 143 aa. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Contains possible hydrophobic membrane spanning region 2 3 21 10 [Reporter]SCO3247 (13B10)_PCR/4985 [Gene]SCO3247/4547 NC_003888 SCE29.16c, possible acyl CoA oxidase, len: 600 aa; similar to many acyl CoA oxidases, especially from eukaryotes e.g. SW:CAOP_RAT acyl CoA oxidase from Rattus norvegicus (rat) (661 aa) fasta scores; opt: 462, z-score: 477.6, E(): 2.7e-19, (27.8% identity in 425 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). 2 3 21 9 [Reporter]SCO0157 (14N6)_PCR/4984 [Gene]SCO0157/4546 NC_003888 SCJ1.06, doubtful CDS, len: 125 aa 2 3 21 8 [Reporter]SCO2873 (15J6)_PCR/4983 [Gene]SCO2873/4545 NC_003888 SCE6.10c, unknown, len: 181 aa. Shows weak similarity to Streptomyces coelicolor TR:CAB51981 (EMBL:AL109663) hypothetical protein, SC4A10.05C (160 aa), fasta scores opt: 171 z-score: 221.2 E(): 6.9e-05 32.7% identity in 156 aa overlap. 2 3 21 7 [Reporter]SCO3707 (16F6)_PCR/4982 [Gene]SCO3707/4544 NC_003888 SCH35.17, putative lipoprotein, len: 189 aa; weakly similar to TR:Q54334 (EMBL:U22894) hypothetical protein from the AUD1 region of Streptomyces lividans 66 (184 aa) fasta scores; opt: 127, z-score: 144.8, E(): 0.98, (28.4% identity in 162 aa overlap). Also weakly similar to SW:T2D8_HUMAN transcription initiation factor from Homo sapiens (human) (218 aa) fasta scores; opt: 152,, z-score: 169.1 E(): 0.044, (32.3% identity in 167 aa overlap). Also contains properly situated Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 2 3 21 6 [Reporter]SCO4977 (17B6)_PCR/4981 [Gene]SCO4977/4543 NC_003888 2SCK31.37c, probable tetR-family transcriptional regulator, len: 235 aa; similar to SW:TCMR_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C transcriptional repressor TcmR, 226 aa; fasta scores: opt: 304 z-score: 354.3 E(): 3.4e-12; 37.2% identity in 218 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 46..67 (+5.26 SD) 2 2 4 18 [Reporter]SCO7076 (4N23)_PCR/2603 [Gene]SCO7076/2369 NC_003888 SC3A4.02, putative two-component histidine kinase, len: 483 aa. Similar in parts to Agrobacterium tumefaciens SW:CHVG_AGRTU(EMBL:L18860) putative two-component histidine kinase required for virulence (690 aa), fasta scores, opt:399 z-score: 426.8 E(): 2.6e-16 33.1% identity in 357 aa overlap. Also similar to Streptomyces coelicolor SCD84.23c (481 aa), fasta scores opt: 874 z-score: 748.4 E():0 39.8% identity in 480 aa overlap. Contains a Pfam match to entry PF00672 DUF5, Domain found in bacterial signal proteins and a Pfam match to entry PF00512 signal, Histidine kinase. Contains probable membrane-spanning domain 2 3 21 5 [Reporter]SCO0341 (18N2)_PCR/4980 [Gene]SCO0341/4542 NC_003888 SCF12.20c, possible large secreted protein, len: 809 aa; similar to TR:CAB36703 (EMBL:AL035521) hypothetical protein from Arabidopsis thaliana (thale cress) (847 aa) fasta scores; opt: 903, z-score: 959.2, E(): 0, 38.7% identity in 775 aa overlap 2 2 4 17 [Reporter]SCO7065 (5J23)_PCR/2602 [Gene]SCO7065/2368 NC_003888 SC4G1.31, conserved hypothetical protein, len: 123 aa; similar to TR:Q9X7R3 (EMBL:AL049863) Streptomyces coelicolor hypothetical 11.4 kDa protein SC5H1.05, 117 aa; fasta scores: opt: 153 z-score: 212.5 E(): 0.00023; 37.1% identity in 116 aa overlap 2 3 21 4 [Reporter]SCP1.240c (19J2)_PCR/4979 [Gene]SCP1.240c/4541 NC_003888 SCP1.240c, mmyO, possible monooxygenase, len: 373aa; previously sequenced and annotated as putative alkanal monooxygenase TR:Q9JN87 (EMBL:AJ276673). Similar to weakly similar to SW:P07740 (LUXA_VIBHA) alkanal monooxygenase alpha chain (EC 1.14.14.3) from Vibrio harveyi (355 aa) fasta scores; opt: 215, z-score: 254.6, E(): 1e-06, 22.1% identity in 272 aa overlap. Contains Pfam match to entry PF00296 bac_luciferase, Bacterial luciferase. 2 1 10 11 [Reporter]SCO4438 (11J24)_PCR/712 [Gene]SCO4438/649 NC_003888 SCD6.16c, possible integral membrane protein, len: 625 aa; similar to TR:Q9KXI6 (EMBL:AL357591) Streptomyces coelicolor putative integral membrane protein SCC53.25, 551 aa; fasta scores: opt: 1266 Z-score: 1316.1 bits: 253.5 E(): 1.1e-65; 42.430% identity in 502 aa overlap. Contains possible hydrophobic membrane spanning regions 2 2 4 16 [Reporter]SCO6726 (6F23)_PCR/2601 [Gene]SCO6726/2367 NC_003888 SC5F2A.09, possible endonuclease, len: 233aa; similar to SW:NFI_ECOLI endonuclease V from Escherichia coli (223 aa) fasta scores; opt: 468, z-score: 541.7, E(): 7.4e-23, (38.6% identity in 215 aa overlap). 2 3 21 3 [Reporter]SCP1.233 (20F2)_PCR/4978 [Gene]SCP1.233/4540 NC_003888 SCP1.233, mmyD, hypothetical protein, len: 339aa; previously sequenced and annotated as TR:Q9JN80 (EMBL:AJ276673). Similar to TR:Q52426 (EMBL:L11334) AvrD, avirulence protein from Pseudomonas syringae (311 aa) fasta scores; opt: 253, z-score: 309.5, E(): 9e-10, 31.4% identity in 334 aa overlap. 2 1 10 10 [Reporter]SCO3508 (12F24)_PCR/711 [Gene]SCO3508/648 NC_003888 SCE134.09, possible maturase-related protein, len: 145aa; similar to many proposed maturase-related protein from prokaryotes eg. TR:O53616 (EMBL:AL021428) putative maturase homolog from Mycobacterium tuberculosis (235 aa) fasta scores; opt: 251, z-score: 315.3, E(): 3e-10, (56.9% identity in 72 aa overlap). 2 2 4 15 [Reporter]SCO7437 (7B23)_PCR/2600 [Gene]SCO7437/2366 NC_003888 SC6D11.33c, possible decarboxylase, len: 526 aa. Similar to many thiamine pyrophosphate requiring enzymes. Highly similar to Pseudomonas putida SW:MDLC_PSEPU(EMBL:J05293) benzoylformate decarboxylase (EC 4.1.1.7) MdlC (528 aa), fasta scores opt: 1829 z-score: 1915.9 E(): 0 52.6% identity in 521 aa overlap. Also similar to Escherichia coli SW:ILVB_ECOLI(EMBL:J01633) acetolactate synthase isozyme I large subunit (EC 4.1.3.18) (562 aa), fasta scores opt: 495 z-score: 520.7 E(): 1.4e-21 26.0% identity in 523 aa overlap. Contains a Prosite hit to PS00187 Thiamine pyrophosphate enzymes signature and a Pfam match to entry PF00205 TPP_enzymes, Thiamine pyrophosphate enzymes. 2 1 10 9 [Reporter]SCO3465 (13B24)_PCR/710 [Gene]SCO3465/647 NC_003888 SCE65.01, hypothetical protein (partial CDS), len: >38 aa; identical to previously sequenced TR:BAA84086 (EMBL:AB032065) Streptomyces ceolicolor hypothetical 47.9 KD protein, 446 aa,SCE46.22, hypothetical protein, len: >231 aa; identical to C-terminal region of previously sequenced TR:BAA84086 (EMBL:AB032065) Streptomyces coelicolor hypothetical 47.9 kD protein, 446 aa. Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases 2 2 4 14 [Reporter]SCO7602 (8N19)_PCR/2599 [Gene]SCO7602/2365 NC_003888 SC7H9.14, probable tetR-family transcriptional regulator, len: 195 aa; similar to TR:O53323 (EMBL:AL021646) Mycobacterium tuberculosis putative regulatory protein MTV014.17c, 200 aa; fasta scores: opt: 319 z-score: 395.7 E(): 1.7e-14; 33.3% identity in 195 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Also contains possible helix-turn-helix motif at residues 36..57 (+3.70 SD) 2 1 10 8 [Reporter]SCO0662 (14N20)_PCR/709 [Gene]SCO0662/646 NC_003888 SCF91.22, possible binding protein dependent transport protein, len: 287 aa. Similar to many membrane transport proteins e.g. Agrobacterium radiobacter SW:LACG_AGRRD(EMBL:X66596) lactose transport system permease protein (273 aa), fasta scores opt: 439 z-score: 546.1 E(): 4.8e-23 28.5% identity in 267 aa overlap and Bacillus subtilis TR:O05095(EMBL:X89810) putative integral protein (281 aa), fasta scores opt: 480 z-score: 596.3 E(): 7.7e-26 30.8% identity in 266 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and multiple possible membrane spanning hydrophobic domains. 2 2 4 13 [Reporter]SCO6445 (9J19)_PCR/2598 [Gene]SCO6445/2364 NC_003888 SC9B5.12, probable inositol monophosphatase, len: 281aa; similar to many eg. SW:MYO3_LYCES myo-inositol-1(or 4)-monophosphatase 3 from Lycopersicon esculentum (tomato) (268 aa) fasta scores; opt: 286, z-score: 396.1, E(): 9e-15, (30.9% identity in 275 aa overlap). Contains PS00629 Inositol monophosphatase family signature 1 and PS00630 Inositol monophosphatase family signature 2. Contains 2x Pfam match to entry PF00459 inositol_P, Inositol monophosphatase family. 2 1 10 7 [Reporter]SCO0118 (15J20)_PCR/708 [Gene]SCO0118/645 NC_003888 SCJ11.47, possible xylosidase/arabinosidase, len: 509aa; similar to many xylosidase/arabinosidases especially in the N-terminal xylosidase region e.g. SW:XYLB_BUTFI xylosidase/arabinosidase from Butyrivibrio fibrisolvens (517 aa) fasta scores; opt: 582, z-score: 640.5, E(): 2.6e-28, (35.2% identity in 526 aa overlap) 2 2 4 12 [Reporter]SCO2297 (10F19)_PCR/2597 [Gene]SCO2297/2363 NC_003888 SCC30.05, hypothetical protein, len: 260 aa; similar to SW:YHI3_CAMJE (EMBL:Z36940) Campylobacter jejuni hypothetical 28.7 kD protein in HipO 3'region, 246 aa; fasta scores: opt: 245 z-score: 295.9 E(): 4.9e-09; 28.1% identity in 210 aa overlap 2 1 10 6 [Reporter]SCO5009 (16F20)_PCR/707 [Gene]SCO5009/644 NC_003888 SCK15.11, probable secretory protein, len: 445 aa; similar to TR:P94647 (EMBL:U77780) Chlorobium limicola secretory protein kinase Kbh, 474 aa; fasta scores: opt: 1192 z-score: 1311.8 E(): 0; 47.7% identity in 396 aa overlap, to SW:TRBB_AGRT6 (EMBL:U43675) Agrobacterium tumefaciens conjugal transfer protein TrbB, 323 aa; fasta scores: opt: 512 z-score: 568.9 E(): 3.2e-24; 30.4% identity in 326 aa overlap and to TR:Q9X921 (EMBL:AL035636) Streptomyces coelicolor putative secretory protein SCH5.19c, 523 aa; fasta scores: opt: 878 z-score: 847.4 E(): 0; 40.3% identity in 365 aa overlap. Contains Pfam match to entry PF00437 GSPII_E, Bacterial type II secretion system protein and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 2 4 11 [Reporter]SCO4585 (11B19)_PCR/2596 [Gene]SCO4585/2362 NC_003888 SCD20.03, probable ABC transporter ATP-binding protein, len: 327 aa; similar to SW:BCRA_BACLI (EMBL:L20573) Bacillus licheniformis bacitracin transport ATP-binding protein BcrA, 306 aa; fasta scores: opt: 636 z-score: 662.0 E(): 2.1e-29; 39.6% identity in 308 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 2 1 10 5 [Reporter]SCO1083 (17B20)_PCR/706 [Gene]SCO1083/643 NC_003888 SCG22.29c, probable flavin-dependent reductase, len: 169 aa; similar to TR:O68503 (EMBL:AF048979) Rhodococcus erythropolis NADH-dependent FMN oxydoreductase DszD, 192 aa; fasta scores: opt: 444 z-score: 517.7 E(): 2.3e-21; 44.6% identity in 168 aa overlap. Contains Pfam match to entry PF01613 Flavin_Reduct, Flavin reductase like domain 2 2 4 10 [Reporter]SCO3298 (12N15)_PCR/2595 [Gene]SCO3298/2361 NC_003888 SCE15.15, possible oxidoreductase, len: 260aa; similar to many egs. SW:YV10_MYCTU putative oxidoreductase from Mycobacterium tuberculosis (251 aa) fasta scores; opt: 826, z-score: 880.1, E():0, (57.2% identity in 243 aa overlap) and TR:O54197 (EMBL:AJ000671) clavulanate-9-aldehyde reductase from Streptomyces clavuligerus (247 aa) fasta scores; opt: 493, z-score: 530.5, E(): 3.1e-22, (39.8% identity in 249 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 2 1 10 4 [Reporter]SCP1.01c (18N16)_PCR/705 [Gene]SCP1.01c/642 NC_003888 SCP1.01c, unknown, len: 207aa; 2 2 4 9 [Reporter]SCO0498 (13J15)_PCR/2594 [Gene]SCO0498/2360 NC_003888 SCF34.17c, probable peptide monooxygenase, len: 451 aa; similar to monooxygenases/hydroxylases associated with peptide siderophore biosynthesis e.g. SW:PVDA_BURCE (EMBL:AF013993), PvdA, Burkholderia cepacia L-ornithine 5-monooxygenase (444 aa), fasta scores; opt: 982 z-score: 1149.4 E(): 0, 43.5% identity in 405 aa overlap and SW:IUCD_ECOLI (EMBL:M18968), IucD, Escherichia coli L-lysine 6-monooxygenase (425 aa) (31.3% identity in 425 aa overlap). Also similar to others from S.coelicolor e.g. TR:Q9ZBH9 (EMBL:AL035206) S.coelicolor probable oxidoreductase, partial CDS (353 aa) (38.1% identity in 349 aa overlap) 2 1 10 3 [Reporter]SCO4888 (19J16)_PCR/704 [Gene]SCO4888/641 NC_003888 2SCK8.14, probable sugar ABC transporter integral membrane protein, len: 424 aa; similar to TR:Q9WXW0 (EMBL:AE001696) Thermotoga maritima sugar ABC transporter, permease protein TM0105, 319 aa; fasta scores: opt: 395 z-score: 433.3 E(): 1.4e-16; 36.9% identity in 279 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 10 2 [Reporter]SCO2817 (20B16)_PCR/703 [Gene]SCO2817/640 NC_003888 SCBAC17F8.08c, conserved hypothetical protein, len: 634 aa: similar in regions to many proteins of varying lengths e.g. TR:Q9RCY2 (EMBL:AL133469) hypothetical protein SCM10.16c from Streptomyces coelicolor (714 aa) fasta scores; opt: 1259, Z-score: 1358.4, 43.960% identity (46.454% ungapped) in 596 aa overlap and TR:Q9HVI8 (EMBL:AE004874) hypothetical protein from Pseudomonas aeruginosa (1415 aa) fasta scores; opt: 961, Z-score: 1032.5, 36.825% identity (40.250% ungapped) in 611 aa overlap. Contains Pfam match to entry PF00563 EAL, Domain of unknown function and Pfam match to entry PF00990 GGDEF, GGDEF domain 3 4 15 12 [Reporter]SCO2277 (11C5)_PCR/7374 [Gene]SCO2277/6719 NC_003888 SCC75A.23, possible membrane protein, len: 290 aa. Highly similar to many proteins of undefined function including: Escherichia coli SW:YCDN_ECOLI(EMBL:AE000203) YcdN (276 aa), fasta scores opt: 693 z-score: 817.1 E():0 41.6% identity in 267 aa overlap and Synechocystis sp. (strain PCC 6803) SW:Y964_SYNY3(EMBL:D90901) (308 aa), fasta scores opt: 363 z-score: 431.0 E(): 1.2e-16 30.4% identity in 303 aa overlap 3 4 15 11 [Reporter]SCO4493 (12O1)_PCR/7373 [Gene]SCO4493/6718 NC_003888 SCD69.13, probable asnC-family transcriptional regulator, len: 167 aa; similar to SW:LRPC_BACSU (EMBL:AB001488) Bacillus subtilis transcriptional regulator LrpC, 144 aa; fasta scores: opt: 249 z-score: 304.8 E(): 1.6e-09; 36.0% identity in 125 aa overlap. Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family and match to Prosite entry PS00519 Bacterial regulatory proteins, asnC family signature. Contains a possible helix-turn-helix motif at residues 33..45 (+2.79 SD) and also a TTA leucine codon, possible target for bldA regulation 3 4 15 10 [Reporter]SCO3020 (13K1)_PCR/7372 [Gene]SCO3020/6717 NC_003888 SCE34.01c, possible integral membrane protein, len: 335 aa; similar to TR:O69662 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical 35.5 kD protein MTV025.042c, 330 aa; fasta scores: opt: 1119 z-score: 1285.3 E(): 0; 51.8% identity in 334 aa overlap. Contains possible hydrophobic membrane spanning regions 3 4 15 9 [Reporter]SCO0581 (14G1)_PCR/7371 [Gene]SCO0581/6716 NC_003888 SCF55.05c, probable transporter protein, len: 455 aa; similar to SW:TUB4_AGRVI (EMBL:U25634) Agrobacterium vitis putative tartrate transporter Tub4, 433 aa; fasta scores: opt: 894 z-score: 1016.3 E(): 0; 42.8% identity in 432 aa overlap and to SW:PHT1_PSEPU (EMBL:D13229) Pseudomonas putida phthalate transporter Pht1, 451 aa; fasta scores: opt: 745 z-score: 847.5 E(): 0; 30.8% identity in 435 aa overlap. Contains match to Pfam entry PF00083 sugar_tr, Sugar (and other) transporter 3 4 15 8 [Reporter]SCO3349 (15C1)_PCR/7370 [Gene]SCO3349/6715 NC_003888 SCE7.16, unknown, len: 271aa; similar to SW:YV21_MYCTU hypothetical protein from Mycobacterium tuberculosis (296 aa) fasta scores; opt: 966, z-score: 1180.4, E(): 0, (50.4% identity in 278 aa overlap) 3 4 15 7 [Reporter]SCO0215 (15O21)_PCR/7369 [Gene]SCO0215/6714 NC_003888 SCJ12.27c, unknown, len: 335 aa. Similar to several hypotheticals e.g. Mycobacterium tuberculosis TR:P95195 (EMBL; Z83867) hypothetical 36.0 KD protein (332 aa), fasta scores opt: 441 z-score: 491.9 E(): 4.9e-20 35.7% identity in 319 aa overlap and S. coelicolor (AL109962) hypothetical protein SCJ1.11 (330 aa), fasta scores opt: 816 z-score: 796.1 E():0 43.0% identity in 321 aa overlap and SCJ12.14 (309 aa), fasta scores opt: 780 z-score: 755.6 E(): 0 45.8% identity in 308 aa overlap. 3 4 15 6 [Reporter]SCO3330 (16K21)_PCR/7368 [Gene]SCO3330/6713 NC_003888 SCE68.28c, probable acetoin utilization protein, len: 367 aa; similar to proteins known to be required for growth on acetoin and 2,3-butanediol in Bacillus subtilis and Staphylococcus xylosus e.g. SW:ACUC_BACSU (EMBL:L17309), AcuC, Bacillus subtilis acetoin utilization protein (387 aa), fasta scores; opt: 910 z-score: 1032.3 E(): 0, 43.5% identity in 315 aa overlap. Similar to many histone deacetylases e.g. SW:RPD3_YEAST (EMBL:S66438), Rpd3, Saccharomyces cerevisiae histone deacetylases (433 aa) (32.0% identity in 338 aa overlap). Contains Pfam match to entry PF00850 Hist_deacetyl, Histone deacetylase family 3 4 15 5 [Reporter]SCO0444 (17G21)_PCR/7367 [Gene]SCO0444/6712 NC_003888 SCF51A.22c, possible integral membrane protein, len: 133 aa. Contains possible membrane spanning hydrophobic domains. 2 3 21 2 [Reporter]SCO5070 (20N22)_PCR/4977 [Gene]SCO5070/4539 NC_003888 SCBAC20F6.13c, ORFB hydroxylacyl-CoA dehydrogenase, len: 394 aa; identical to previously sequenced TR:Q53924 (EMBL:X62373) Streptomyces coelicolor hydroxylacyl-CoA dehydrogenase ORFB, 394 aa. There is an alternative start codon 120 base pairs upstream of the previously sequenced one 3 4 15 4 [Reporter]SCO0553 (18C21)_PCR/7366 [Gene]SCO0553/6711 NC_003888 SCF11.33c, unknown, len: 145 aa 2 3 20 22 [Reporter]SCO1341 (1N18)_PCR/4975 [Gene]SCO1341/4538 NC_003888 2SCG61.23, possible lipoprotein, len: 168aa; contains an appropriately Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible non-cleavable N-terminal signal sequence. 3 4 15 3 [Reporter]SCO1862 (19O17)_PCR/7365 [Gene]SCO1862/6710 NC_003888 SCI39.09c, possible integral membrane protein, len: 160 aa. Contains possible hydrophobic membrane spanning regions 2 3 20 21 [Reporter]SCO1476 (2J18)_PCR/4974 [Gene]SCO1476/4537 NC_003888 SCL6.33c, metK, S-adenosylmethionine synthetase, len: 402 aa; identical to TR:Q9X4Q2 (EMBL:AF117274) Streptomyces spectabilis S-adenosylmethionine synthetase (EC 2.5.1.6) MetK, 411 aa and highly similar to SW:METK_BACSU (EMBL:U52812) Bacillus subtilis S-adenosylmethionine synthetase (EC 2.5.1.6) MetK, 400 aa; fasta scores: opt: 1583 z-score: 1723.1 E(): 0; 61.9% identity in 402 aa overlap. Contains Pfam match to entry PF00438 S-AdoMet_synt, S-adenosylmethionine synthetase and matches to Prosite entries PS00376 S-adenosylmethionine synthetase signature 1 and PS00377 S-adenosylmethionine synthetase signature 2 2 3 20 20 [Reporter]SCO7650 (3F18)_PCR/4973 [Gene]SCO7650/4536 NC_003888 SC10F4.23, possible secreted hydrolase, len: 490 aa; similar to TR:Q9ZBJ5 (EMBL:AL035161) Streptomyces coelicolor putative secreted peptidase SC9C7.15c, 529 aa; fasta scores: opt: 678 z-score: 721.7 E(): 1.2e-32; 36.6% identity in 514 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold and possible N-terminal region signal peptide sequence 2 3 20 19 [Reporter]SCO5604 (4B18)_PCR/4972 [Gene]SCO5604/4535 NC_003888 SC2E1.21, unknown, len: 382 aa; N-terminus is Gly-rich, C-terminus is similar to the N-terminus of several hypothetical proteins e.g. SW:SMF_ECOLI SMF protein (374 aa), fasta scores; opt: 402 z-score: 277.5 E(): 3.2e-08, 40.7% identity in 194 aa overlap. This ORF appears to be interrupted by the following prophage at around aa 360, and to continue as the last part of SC2E1.37. The similarity to the M. tuberculosis hypothetical protein SW:YX27_MYCTU MTCY274.27c (389 aa) (fasta scores; opt: 603 z-score: 511.5 E(): 3e-21, 46.8% identity in 267 aa overlap) stops at that position and restarts in SC2E1.37. Alternative start site at aa 118 sugested by positional base preference 2 3 20 18 [Reporter]SCO7677 (5N14)_PCR/4971 [Gene]SCO7677/4534 NC_003888 SC4C2.12, possible secreted solute-binding protein, len: 528 aa; similar to TR:P96429 (EMBL:Z79692) Sinorhizobium meliloti periplasmic binding protein ORF23, 537 aa; fasta scores: opt: 644 z-score: 679.5 E(): 2.6e-30; 28.8% identity in 525 aa overlap. Contains Pfam match to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5 and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 2 3 20 17 [Reporter]SCO6747 (6J14)_PCR/4970 [Gene]SCO6747/4533 NC_003888 SC5F2A.30, unknown, len: 211aa; similar to TR:P71709 (EMBL:Z80775) hypothetical protein from Mycobacterium tuberculosis (187 aa) fasta scores; opt: 529, z-score: 638.6, E(): 2.9e-28, (49.5% identity in 182 aa overlap) and to TR:AAK44280 (EMBL:AE006918) Mycobacterium tuberculosis CDC1551 4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis, putative MT0058, 212 aa; fasta scores: opt: 619 Z-score: 715.6 E(): 3.2e-32; 50.242% identity in 207 aa overlap 2 2 4 8 [Reporter]SCO3303 (14F15)_PCR/2593 [Gene]SCO3303/2359 NC_003888 SCE68.01c, possible lysyl-tRNA synthetase, len: >76 aa; similar to SW:SYK_METMP (EMBL:AF009824) LysS, Methanococcus maripaludis lysyl-tRNA synthetase (533 aa), fasta scores; opt: 114 z-score: 145.7 E(): 0.86, 44.7% identity in 38 aa overlap and to many hypothetical lysyl-tRNA synthetases. The remainder of this CDS is SCE15.20c, partial CDS, possible lysyl-tRNA in EMBL:AL049707,SCE15.20c, partial CDS, possible lysyl-tRNA synthetase, len: >545aa; similar to many eg. SW:SYK_METJA lysyl-tRNA synthetase from Methanococcus jannaschii (530 aa) fasta scores; opt: 419, z-score: 468.7, E(): 8.5e-19, (29.2% identity in 559 aa overlap). Contains 2 3 20 16 [Reporter]SCO5632 (7F14)_PCR/4969 [Gene]SCO5632/4532 NC_003888 SC6A9.35, unknown, len: 80 aa 2 2 4 7 [Reporter]SCO4651 (15B15)_PCR/2592 [Gene]SCO4651/2358 NC_003888 SCD82.22, yjr2, possible lipoprotein, len: 282 aa; identical to previously sequenced SW:YRJ2_STRCO (EMBL:L24552) Streptomyces coelicolor hypothetical 30.0 kD protein in rplJ 5' region (ORF2), 282 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and to entry PS00017 ATP/GTP-binding site motif A (P-loop), also contains possible N-terminal region signal peptide sequence 2 3 20 15 [Reporter]SCO5215 (8B14)_PCR/4968 [Gene]SCO5215/4531 NC_003888 SC7E4.12, hypothetical protein, len: 211 aa; similar to TR:P96267 (EMBL:Z84724) Mycobacterium tuberculosis hypothetical 21.7 kD protein MTCY22G10.18c, 209 aa; fasta scores: opt: 255 z-score: 287.1 E(): 1.6e-08; 29.8% identity in 205 aa overlap 2 1 9 22 [Reporter]SCO2804 (1B12)_PCR/701 [Gene]SCO2804/639 NC_003888 2SCC13.12c, hypothetical protein, len: 131 aa; similar to TR:O86827 (EMBL:AL031225) Streptomyces coelicolor hypothetical 15.7 kDa protein SC8B7.06c, 152 aa; fasta scores: opt: 232 z-score: 266.8 E(): 2.4e-07; 41.6% identity in 113 aa overlap 2 2 4 6 [Reporter]SCO0263 (16N11)_PCR/2591 [Gene]SCO0263/2357 NC_003888 SCF1.05c, possible oxidoreductase, len: 371 aa; weakly similar to many e.g. TR:O69945 (EMBL:AL023862) putative oxidoreductase from Streptomyces coelicolor (430 aa) fasta scores; opt: 224, z-score: 247.7, E(): 2e-06, (26.0% identity in 392 aa overlap). Contains Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family. 2 3 20 14 [Reporter]SCO7005 (9N10)_PCR/4967 [Gene]SCO7005/4530 NC_003888 SC8F11.31, possible oxidoreductase, len: 467 aa. Similar to several including: Homo sapiens TR:AAF21941(EMBL:AF111858) dimethylglycine dehydrogenase precursor (EC 1.5.99.2) (866 aa), fasta scores opt: 217 z-score: 242.0 E(): 4.7e-06 24.7% identity in 372 aa overlap and Pyrococcus abyssi TR:CAB49193(EMBL:AJ248283) glycerol-3-phosphate dehydrogenase, GlpA (497 aa), fasta scores opt: 787 z-score: 883.5 E(): 0 36.1% identity in 454 aa overlap. Contains a Pfam match to entry PF01224 FAD_Gly3P_dh, FAD-dependent glycerol-3-phosphate dehydrogenase. Also contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 2 1 9 21 [Reporter]SCO7234 (2N8)_PCR/700 [Gene]SCO7234/638 NC_003888 SC2H12.33, ctaD, cytochrome C oxidase polypeptide I (EC 1.9.3.1), len: 573 aa. Highly similar to many e.g. Bacillus subtilis SW:COX1_BACSU(EMBL:X54140) cytochrome C oxidase polypeptide I (EC 1.9.3.1) CtaD (621 aa), fasta scores opt: 1725 z-score: 1882.8 E():0 47.7% identity in 524 aa overlap and Streptomyces coelicolor TR:Q9X813(EMBL:AL049497) putative cytochrome C oxidase subunit I (EC 1.9.3.1), SC6G10.28C (578 aa), fasta scores opt: 3002 z-score: 3275.1 E():0 75.6% identity in 541 aa overlap. Contains a Prosite hit to PS00077 Heme-copper oxidase catalytic subunit, copper B binding region signature and a Pfam match to entry PF00115 COX1, Cytochrome C and Quinol oxidase polypeptide I. Also contains multiple possible membrane spanning hydrophobic domains 2 2 4 5 [Reporter]SCO5157 (17J11)_PCR/2590 [Gene]SCO5157/2356 NC_003888 SCP8.20c, probable metal-transport protein, len: 371 aa; similar to TR:O50455 (EMBL:AL021006) Mycobacterium tuberculosis putative magnesium and cobalt transport protein MTV006.11c, 366 aa; fasta scores: opt: 1137 z-score: 1293.8 E(): 0; 47.8% identity in 370 aa overlap and to SW:CORA_ECOLI (EMBL:L11042) Escherichia coli magnesium and cobalt transport protein CorA, 316 aa; fasta scores: opt: 211 z-score: 245.7 E(): 3.2e-06; 23.2% identity in 306 aa overlap. Contains Pfam match to entry PF01544 CorA, CorA-like Mg2+ transporter protein and possible hydrophobic membrane spanning region at C-terminal domain 2 1 9 20 [Reporter]SCO4660 (3J8)_PCR/699 [Gene]SCO4660/637 NC_003888 SCD40A.06, rpsG, 30S ribosomal protein S7, len: 156 aa; highly similar to SW:RS7_STRRP (EMBL:U60191) Streptomyces roseosporus 30S ribosomal protein S7 RpsG, 156 aa; fasta scores: opt: 979 z-score: 1165.5 E(): 0; 94.9% identity in 156 aa overlap. Contains Pfam match to entry PF00177 Ribosomal_S7, Ribosomal protein S7p/S5e and match to Prosite entry PS00052 Ribosomal protein S7 signature 2 2 4 4 [Reporter]SCO3543 (18F11)_PCR/2589 [Gene]SCO3543/2355 NC_003888 SCH5.06c, probable DNA topoisomerase I, len: 952 aa; high levels of similarity to many e.g. SW:TOP1_MYCTU DNA topoisomerase I from Mycobacterium tuberculosis (900 aa) fasta scores; opt: 2586, z-score: 2586.7, E(): 0, (58.6% identity in 846 aa overlap). Contains PS00396 Prokaryotic DNA topoisomerase I active site and Pfam match to entry PF01131 Topoisom_bac, Prokayotic DNA topoisomerase, score 736.60, E-value 1.1e-217. Also contains alanine/lysine rich C-terminal region. 2 1 9 19 [Reporter]SCO5531 (4F8)_PCR/698 [Gene]SCO5531/636 NC_003888 SC1C2.12c, probable integral membrane protein, len: 334 aa; similar to TR:Q98DR8 (EMBL:AP003004) Rhizobium loti (Mesorhizobium loti) putative integral membrane protein MLL4580, 308 aa; fasta scores: opt: 503 Z-score: 515.3 E(): 4.5e-21; 33.904% identity in 292 aa overlap. COntains possible hydrophobic membrane spanning regions 2 2 4 3 [Reporter]SCO6004 (19B11)_PCR/2588 [Gene]SCO6004/2354 NC_003888 SC7B7.01c, putative secreted ATP/GTP binding protein, partial ORF, len: >626 aa; contains possible N-terminal signal sequence and PS00017 ATP/GTP-binding site motif A (P-loop),SCBAC1C11.07c, conserved hypothetical protein (fragment), len: >676 aa; similar to C-terminal domain of TR:Q9WXI8 (EMBL:AB025248) Bacillus sp. M-90 alpha-1,2-mannosidase precursor AmaN2, 1976 aa; fasta scores: opt: 1575 z-score: 1715.3 E(): 0; 45.7% identity in 705 aa overlap 2 1 9 18 [Reporter]SCO6383 (5B8)_PCR/697 [Gene]SCO6383/635 NC_003888 SC3C8.02c, probable integral membrane protein, len: 227 aa 2 2 4 2 [Reporter]SCO0965 (20J7)_PCR/2587 [Gene]SCO0965/2353 NC_003888 SCM11.20c, conserved hypothetical protein, len: 219 aa; similar to TR:O50637 (EMBL:AB010203) Leptospira interrogans hypothetical 23.2 kD protein, 216 aa; fasta scores: opt: 197 z-score: 222.5 E(): 5.1e-05; 27.5% identity in 182 aa overlap and to TR:Q98N90 (EMBL:AP002994) Rhizobium loti (Mesorhizobium loti) MLL0243 protein, 231 aa; fasta scores: opt: 545 Z-score: 593.3 E(): 2e-25; 48.00% identity in 200 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 9 17 [Reporter]SCO6676 (6N4)_PCR/696 [Gene]SCO6676/634 NC_003888 SC5A7.26, unknown, len: 235 aa 2 2 3 22 [Reporter]SCO4188 (1J3)_PCR/2585 [Gene]SCO4188/2352 NC_003888 2SCD46.02, possible GntR-family transcriptional regulator, len: 303aa; similar to many eg. TR:Q9RIQ2 (EMBL:AJ243257) KorA protein from plasmid pSNA1 of Streptomyces natalensis (245 aa) fasta scores; opt: 175, z-score: 204.2, E(): 0.00069, 28.2% identity in 238 aa overlap. Also strongly similar to neighbouring CDS 2SCD46.04 fasta scores; opt: 1074, z-score: 984.2, E(): 0, 57.6% identity in 302 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and helix-turn-helix motif (Score 1967 (+5.89 SD)) at residue 46-67. Note possible alternative downstream translational start codons. 2 1 9 16 [Reporter]SCO3619 (7J4)_PCR/695 [Gene]SCO3619/633 NC_003888 SC66T3.30c, hypothetical protein, len: 115 aa; unknown function, similar to other hypothetical proteins with Asn-rich N-termini found in the recR regions of some organisms (e.g. Bacillus subtilis, Mycobacterium leprae, Mycobacterium tuberculosis, Escherichia coli) e.g. TR:O69683 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical protein (113 aa), fasta scores; opt: 375 z-score: 409.0 E(): 1.9e-15, 50.8% identity in 120 aa overlap. Contains probable coiled-coils from 9 to 41 (33 residues) (Max score: 1.832, probability 1.00) and from 68 to 96 (29 residues) (Max score: 1.491, probability 0.94) 2 2 3 21 [Reporter]SCO0741 (2F3)_PCR/2584 [Gene]SCO0741/2351 NC_003888 3SC5B7.19c, probable oxidoreductase, len: 362 aa; highly similar to SW:FADH_AMYME Amycolatopsis methanolica NAD/mycothiol-dependent formaldehyde dehydrogenase (EC 1.2.1.-) FadH, 360 aa; fasta scores: opt: 1833 z-score: 1972.8 E(): 0; 74.4% identity in 359 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and matches to Prosite entries PS00190 Cytochrome c family heme-binding site signature, PS00059 Zinc-containing alcohol dehydrogenases signature and PS00435 Peroxidases proximal heme-ligand signature 2 1 9 15 [Reporter]SCO6691 (8F4)_PCR/694 [Gene]SCO6691/632 NC_003888 SC4C6.01, probable phospholipase C, partial CDS, len: >501 aa; similar to e.g. SW:PHLN_PSEAE (EMBL:M59304), plcN, Pseudomonas aeruginosa non-hemolytic phospholipase C precursor (692 aa), fasta scores; opt: 1073 z-score: 1149.1 E(): 0, 44.7% identity in 524 aa overlap,SC6G3.07, probable phospholipase C, partial CDS, len: >221 aa; similar to e.g. SW:PHLN_PSEAE (EMBL:M59304), plcN, Pseudomonas aeruginosa non-hemolytic phospholipase C precursor (692 aa), fasta scores; opt: 853 z-score: 1021.1 E(): 0, 51.3% identity in 232 aa overlap. Continues in cosmid 4C6 (EMBL:AL079355) as SC4C6.01 2 2 3 19 [Reporter]SCO6143 (3N23)_PCR/2582 [Gene]SCO6143/2350 NC_003888 SC1A9.07, hypothetical protein, len: 251 aa; similar to TR:O33986 (EMBL:U82823) hypothetical protein from Saccharopolyspora erythraea (266 aa), fasta scores; opt: 740, z-score: 869.0, E(): 0, (53.4% identity in 251 aa overlap) 2 1 9 14 [Reporter]SCO7713 (9B4)_PCR/693 [Gene]SCO7713/631 NC_003888 SC8D11.04, unknown, len: 134 aa. TBparse predicted 2 1 9 13 [Reporter]SCO6471 (9N24)_PCR/692 [Gene]SCO6471/630 NC_003888 SC9C7.07c, probable citratelyase, len: 339 aa; similar to many e.g. SW:CILB_KLEPN citrate lyase beta chain from Klebsiella pneumoniae (289 aa) fasta scores; opt: 187, z-score: 643.3, E(): 1.5e-28, (30.5% identity in 298 aa overlap). 3 4 15 2 [Reporter]SCO0983 (20G17)_PCR/7364 [Gene]SCO0983/6709 NC_003888 SCBAC19F3.10, aceB2, malate synthase, len: 530 aa; strongly similar to many e.g. SW:Q9ZH77 (MASY_STRCL) malate synthase AceB from Streptomyces clavuligerus (541 aa) fasta scores; opt: 1923, Z-score: 2204.2, 58.889% identity (61.390% ungapped) in 540 aa overlap and TR:Q9RKU9 (EMBL:AL132824) malate synthase AceB1 (StAH10.08c) from Streptomyces coelicolor (540 aa) fasta scores; opt: 1930, Z-score: 2212.2, 61.895% identity (63.090% ungapped) in 475 aa overlap. Contains Pfam match to entry PF01274 Malate_synthase, Malate synthase and Prosite match to PS00510 Malate synthase signature. 3 4 14 22 [Reporter]SCO1284 (1G13)_PCR/7362 [Gene]SCO1284/6708 NC_003888 2SCG18.31c, probable aminotransferase (putative secreted protein), len: 461 aa; similar to TR:CAB76097 (EMBL:AL157956) Streptomyces coelicolor acetonitrile amonitransferase ArgD, 402 aa; fasta scores: opt: 783 z-score: 776.7 E(): 0; 41.6% identity in 385 aa overlap and to SW:OAT_BACSU (EMBL:X81802) Bacillus subtilis ornithine amonitransferase (EC 2.6.1.13) RocD, 401 aa; fasta scores: opt: 692 z-score: 687.5 E(): 8.3e-31; 32.4% identity in 380 aa overlap. Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate and match to Prosite entry PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Also contains a 6x degenerate repeat unit: (P/A/G)ST at N-terminal region and possible N-terminal region signal peptide sequence 3 4 14 21 [Reporter]SCO0997 (2C13)_PCR/7361 [Gene]SCO0997/6707 NC_003888 2SCG2.10, ftrD, Fe uptake system integral membrane protein, len: 371 aa; previously sequenced and annotated as TR:Q9X471 (EMBL:AF099015) Streptomyces coelicolor Fe uptake system integral membrane protein FtrD, 374 aa and similar to TR:CAB94639 (EMBL:AL359215) Streptomyces coelicolor putative FecCD-family membrane transport protein SC2H12.15, 368 aa; fasta scores: opt: 942 z-score: 839.0 E(): 0; 45.8% identity in 360 aa overlap. Contains Pfam match to entry PF01032 FecCD_family, FecCD transport family. Also contains possible hydrophobic membrane spanning regions 3 4 14 20 [Reporter]SCO5900 (3O9)_PCR/7360 [Gene]SCO5900/6706 NC_003888 SC10A5.05, probable membrane protein, len: 691; high ly similar to M. tuberculosis putative membrane protein TR: O07192 (EMBL:Z96072) MTCY05A6.11C (647 aa), fasta scores; o pt: 1980 z-score: 3557.6 E(): 0, 62.7% identity in 659 aa o verlap and weakly similar to many transport proteins eg. PR OY_SALTY P37460 proline-specific permease proy from Salmone lla typhimurium (456 aa), fasta scores; opt: 169 z-score: 1 82.8 E(): 0.0055, 24.2% identity in 355 aa overlap 3 4 14 19 [Reporter]SCO6938 (4K9)_PCR/7359 [Gene]SCO6938/6705 NC_003888 SC1G8.10c, probable acyl-CoA dehydrogenase, len: 417 aa. Similar to Bacillus subtilis TR:ACDB_BACSU (EMBL:U29084) acyl-CoA dehydrogenase (EC 1.3.99.-) MmgC (379 aa), fasta scores opt: 651 z-score: 737.5 E(): 0 31.1% identity in 380 aa overlap and Streptomyces coelicolor TR:CAB87216 (EMBL:AL163641) acyl-CoA dehydrogenase, SCC105.10 (386 aa), fasta scores opt: 575 z-score: 651.8 E(): 7.6e-29 31.7% identity in 382 aa overlap. Contains a Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase and contains possible membrane spanning hydrophobic domains. 3 4 14 18 [Reporter]SCO6405 (5G9)_PCR/7358 [Gene]SCO6405/6704 NC_003888 SC3C8.24, possible DNA recombinase, len: 672 aa; some similarity to CISA_BACSU putative DNA recombinase (500 aa), fasta scores; opt: 139 z-score: 221.6 E(): 4.2e-05, 25.8% identity in 383 aa overlap, with stronger similarity around the conserved active site. Contains PS00397 Site-specific recombinases active site, Pfam match to entry recombinase PF00239, Site-specific recombinases, score 52.67 and probable coiled-coil from aa 391 to 428. Also similar to S. coelicolor putative prophage gene SC2E1.37 (E(): 0, 41.1% identity in 689 aa overlap) 3 4 14 17 [Reporter]SCO2067 (6C9)_PCR/7357 [Gene]SCO2067/6703 NC_003888 SC4G6.36, putative membrane protein, len: 266 aa; weakly similar to TR:O69463 (EMBL:AL023635) hypothetical protein from Mycobacterium leprae (258 aa) fasta scores; opt: 250, z-score: 277.4, E(): 4e-08, (27.2% identity in 250 aa overlap) and similar to TR:Q9S2U7 (EMBL:AL096884) Streptomyces coelicolor putative integral membrane protein SC4G6.04c, 275 aa; fasta scores: opt: 519 Z-score: 568.5 E(): 4.9e-24; 34.470% identity in 264 aa overlap. Contains possible hydrophobic membrane spanning region 3 4 14 16 [Reporter]SCO5435 (7O5)_PCR/7356 [Gene]SCO5435/6702 NC_003888 SC6A11.11c, possible two-component sensor kinase, len: 552 aa; similar to many eg. SW:P39272 (DCUS_ECOLI) sensor protein DcuS from Escherichia coli (543 aa) fasta scores; opt: 764, z-score: 809.1, E(): 0, 29.3% identity in 540 aa overlap. Contains Pfam match to entry PF00512 signal, Histidine kinase and possible membrane-spanning hydrophobic regions 2 3 20 13 [Reporter]SCO2567 (10J10)_PCR/4966 [Gene]SCO2567/4529 NC_003888 SCC123.05c, possible integral membrane protein, len: 855 aa. Similar to Bacillus subtilis SW:CME3_BACSU(EMBL:L15202) ComE operon protein 3, required for the binding and uptake of transforming DNA (776 aa), fasta scores opt: 355 z-score: 368.7 E(): 3.8e-13 26.3% identity in 635 aa overlap and Mycobacterium tuberculosis TR:P71729(EMBL:Z81368) hypothetical 52.8 KD protein (514 aa), fasta scores opt: 450 z-score: 470.1 E(): 8.5e-19 35.2% identity in 546 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 3 4 14 15 [Reporter]SCO7244 (8K5)_PCR/7355 [Gene]SCO7244/6701 NC_003888 SC7A12.11, probable long-chain-fatty-acid-CoA ligase, len: 505 aa. Highly similar to many e.g. Escherichia coli SW:LCFA_ECOLI(EMBL:X70994) long-chain-fatty-acid-CoA ligase (EC 6.2.1.3) (561 aa), fasta scores opt: 692 z-score: 795.2 E(): 0 32.3% identity in 538 aa overlap. Contains a Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme. 2 3 20 12 [Reporter]SCO2625 (11F10)_PCR/4965 [Gene]SCO2625/4528 NC_003888 SCC80.10, hypothetical protein, len: 413 aa; similar to TR:CAB94090 (EMBL:AL358692) Streptomyces coelicolor conserved hypothetical protein SCD66.23, 418 aa; fasta scores: opt: 698 z-score: 807.3 E(): 0; 35.8% identity in 419 aa overlap 3 4 14 14 [Reporter]SCO7726 (9G5)_PCR/7354 [Gene]SCO7726/6700 NC_003888 SC8D11.17, possible hydrolase, len: 218 aa; similar to SW:YCAC_ECOLI (EMBL:J03412) Escherichia coli 23.1 kDa protein in DmsC-PflA intergenic region YcaC, 208 aa; fasta scores: opt: 631 z-score: 780.3 E(): 0; 48.5% identity in 200 aa overlap. Contains Pfam match to entry PF00857 Isochorismatase, Isochorismatase family 2 3 20 11 [Reporter]SCO4165 (12B10)_PCR/4964 [Gene]SCO4165/4527 NC_003888 SCD66.02, conserved hypothetical protein, len: 95 aa; similar to TR:Q54084 (EMBL:M29612) Saccharopolyspora erythraea hypothetical 10.2 kD protein, 101 aa; fasta scores: opt: 255 z-score: 316.1 E(): 3.9e-10; 46.3% identity in 95 aa overlap 2 3 20 10 [Reporter]SCO3274 (13N6)_PCR/4963 [Gene]SCO3274/4526 NC_003888 SCE39.24, unknown, len: 148aa; 2 3 20 8 [Reporter]SCO1575 (15F6)_PCR/4961 [Gene]SCO1575/4525 NC_003888 SCL24.11, possible thiamine biosynthesis lipoprotein precursor, len: 246 aa; similar to SW:APBE_TREPA (EMBL:AE001250) Treponema pallidum thiamine biosynthesis lipoprotein ApbE precursor, 362 aa; fasta scores: opt: 224 z-score: 251.2 E(): 1.4e-06; 34.9% identity in 172 aa overlap. Contains possible N-terminal region signal peptide sequence 2 3 20 7 [Reporter]SCO1702 (16B6)_PCR/4960 [Gene]SCO1702/4524 NC_003888 SCI30A.23, possible transcriptional regulator, len: 213 aa; similar to many e.g. TR:Q53901 (EMBL:M64683) regulatory protein from Streptomyces coelicolor (259 aa) fasta scores; opt: 227, z-score: 273.2, E(): 6.9e-08, (32.3% identity in 217 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and Prosite match to PS01081 Bacterial regulatory proteins, tetR family signature. Contains possible helix-turn-helix motif (+5.17 SD) 31-52aa. 2 3 20 6 [Reporter]SCO3700 (17N2)_PCR/4959 [Gene]SCO3700/4523 NC_003888 SCH35.24c, possible heavy metal reductase, len: 137aa; similar to many arsenate reductases eg. SW:ARSC_STAAU ArsC, arsenate reductase from Staphylococcus aureus (131 aa) fasta scores; opt: 311, z-score: 363.4, E(): 6.5e-13, (38.6% identity in 127 aa overlap). Also similar to the C-terminus of TR:P71942 (EMBL:Z80225) hypothetical protein from Mycobacterium tuberculosis (498 aa) fasta scores; opt: 567, z-score: 641.0, E(): 2.2e-28, (65.1% identity in 129 aa overlap). 2 2 3 18 [Reporter]SCO2030 (4J23)_PCR/2581 [Gene]SCO2030/2349 NC_003888 SC3A3.08, hypothetical protein, len: 392 aa; unknown function, shows very weak similarity to SW:YH24_ARCFU (EMBL:AE000984) Archaeoglobus fulgidus hypothetical protein (305 aa), fasta scores; opt: 147 z-score: 168.3 E(): 0.051, 30.3% identity in 333 aa overlap. Shows weak similarity to the neighbouring CDS SC3A3.06, SC3A3.07 and SC3A3.09 e.g. SC3A3.06 possible membrane protein (509 aa) (33.1% identity in 393 aa overlap) 2 3 20 5 [Reporter]SCO3785 (18J2)_PCR/4958 [Gene]SCO3785/4522 NC_003888 SCH63.32c, possible integral membrane protein, len: 409 aa; similar to TR:CAB95784 (EMBL:AL359949) Streptomyces coelicolor putative integral membrane protein 2SCG61.09, 387 aa; fasta scores: opt: 464 z-score: 500.7 E(): 2.3e-20; 32.5% identity in 379 aa overlap. Contains possible hydrophobic membrane spanning regions 2 2 3 17 [Reporter]SCO6553 (5F23)_PCR/2580 [Gene]SCO6553/2348 NC_003888 SC4B5.03c, probable integral membrane efflux protein, len: 504aa; similar to a family of integral membrane transport proteins most of which appear to be concerned with export of antimicrobial compounds, most similar to SW:TCMA_STRGA tetracenomycin C resistance and export protein from Streptomyces glaucescens (538 aa), fasta scores; opt: 790, z-score: 800.0, E(): 0, (33.8% identity in 497 aa overlap). Contains PS00216 Sugar transport proteins signature 1. Also contains several putative membrane spanning regions. 2 3 20 4 [Reporter]SCO6097 (19F2)_PCR/4957 [Gene]SCO6097/4521 NC_003888 SCBAC1A6.21c, cysN, sulfate adenylyltransferase subunit 1, len: 451 aa; similar to N-terminal domain of SW:CYSN_PSEAE (EMBL:AF035608) Pseudomonas aeruginosa CysN/CysC bifunctional enzyme, 633 aa; fasta scores: opt: 1232 z-score: 1300.1 E(): 0; 47.5% identity in 438 aa overlap and to SW:CYSN_ECOLI (EMBL:M74586) Escherichia coli sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) CysN, 475 aa; fasta scores: opt: 1192 z-score: 1259.9 E(): 0; 46.6% identity in 438 aa overlap. Contains Pfam match to entry PF00009 GTP_EFTU, Elongation factor Tu family and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and PS00301 GTP-binding elongation factors signature 2 1 9 12 [Reporter]SCO2691 (10J24)_PCR/691 [Gene]SCO2691/629 NC_003888 SCC61A.12, unknown, len: 385aa; 2 2 3 16 [Reporter]SCO6722 (6B23)_PCR/2579 [Gene]SCO6722/2347 NC_003888 SC5F2A.05c, possible regulator, len: 138aa; similar to TR:P95753 (EMBL:D50051) SsgA protein associated with sporulation and cell division in Streptomyces griseus (136 aa) fasta scores; opt: 233, z-score: 312.5, E(): 4.3e-10, (33.9% identity in 115 aa overlap). 2 3 20 3 [Reporter]SCP1.184 (20B2)_PCR/4956 [Gene]SCP1.184/4520 NC_003888 SCP1.184, conserved hypothetical protein, len: 92aa; strongly similar to SCJ30.10c (TR:Q9S1X9 EMBL:AL109973) hypothetical protein from the chromosome end(s) of Streptomyces coelicolor (92 aa) fasta scores; opt: 499, z-score: 682.6, E(): 1.5e-30, 81.5% identity in 92 aa overlap. Also similar to SCE6.04 (TR:Q9KZT4 EMBL:AL353832) hypothetical protein from Streptomyces coelicolor (75 aa) fasta scores; opt: 107, z-score: 161.9, E(): 0.15, 30.9% identity in 81 aa overlap. Contains a TTA encoded leucine at residue 3, possible target for bldA regulation. 2 1 9 11 [Reporter]SCO4689 (11F24)_PCR/690 [Gene]SCO4689/628 NC_003888 SCD31.14, hypothetical protein, len: 110 aa; similar to TR:O06194 (EMBL:Z95387) Mycobacterium tuberculosis hypothetical 12.3 kD protein MTCY1A10.14, 117 aa; fasta scores: opt: 407 z-score: 548.1 E(): 4.2e-23; 56.0% identity in 109 aa overlap 2 2 3 15 [Reporter]SCO4415 (7N19)_PCR/2578 [Gene]SCO4415/2346 NC_003888 SC6F11.13, possible integral membrane protein, len: 290 aa. Contains possible hydrophobic membrane spanning regions 2 1 9 10 [Reporter]SCO3504 (12B24)_PCR/689 [Gene]SCO3504/627 NC_003888 SCE134.05c, possible binding protein dependent transport protein, len: 327aa; similar to many egs. SW:YURN_BACSU hypothetical ABC transporter permease from Bacillus subtilis (292 aa) fasta scores; opt: 586, z-score: 707.9, E(): 4.1e-32, (32.4% identity in 287 aa overlap) and SW:UGPA_ECOLI Sn-glycerol-3-phosphate transport permease from Escherichia coli (295 aa) fasta scores; opt: 583, z-score: 704.3, E(): 6.5e-32, (33.8% identity in 290 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp signature. 2 2 3 14 [Reporter]SCO7598 (8J19)_PCR/2577 [Gene]SCO7598/2345 NC_003888 SC7H9.10, unknown, len: 38 aa. Doubtful CDS, low content in G+C (63.15%) 2 1 9 9 [Reporter]SCO3706 (13N20)_PCR/688 [Gene]SCO3706/626 NC_003888 SCH35.18c, probable ABC transporter ATP-binding subunit, len: 374aa; similar to many egs. TR:O31231 (EMBL:Y10817) from Arthrobacter nicotinovorans (349 aa) fasta scores; opt: 816, z-score: 875.0, E(): 0, (43.2% identity in 340 aa overlap) and TR:O86751 (EMBL:AL031035) putative ABC transporter ATP-binding subunit from Streptomyces coelicolor (348 aa) fasta scores; opt: 653, z-score: 701.9, E(): 9e-32, (38.6% identity in 321 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite matches to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 2 2 3 13 [Reporter]SCO7156 (9F19)_PCR/2576 [Gene]SCO7156/2344 NC_003888 SC9A4.18c, conserved hypothetical protein, len: 152 aa; similar to TR:Q9RRW9 (EMBL:AE002067) Deinococcus radiodurans conserved hypothetical protein DR2363, 160 aa; fasta scores: opt: 161 z-score: 210.3 E(): 0.0003; 34.0% identity in 147 aa overlap 2 1 9 8 [Reporter]SCO0260 (14J20)_PCR/687 [Gene]SCO0260/625 NC_003888 SCF1.02, unknown, len: 109 aa; similar to TR:O06569 (EMBL:Z95585) hypothetical protein from Mycobacterium tuberculosis (107 aa) fasta scores; opt: 433, z-score: 595.2, E(): 8.7e-26, (56.6% identity in 106 aa overlap) 2 2 3 12 [Reporter]SCO2293 (10B19)_PCR/2575 [Gene]SCO2293/2343 NC_003888 SCC30.01c, possible integral membrane protein, len: 326 aa; similar to TR:BAA87714 (EMBL:AB016260) Agrobacterium tumefaciens TIORF89 protein, 306 aa; fasta scores: opt: 396 z-score: 465.4 E(): 1.8e-18; 28.7% identity in 286 aa overlap. Contains 2x Pfam match to entry PF00892 DUF6, Integral membrane protein and possible hydrophobic membrane spanning regions 2 1 9 7 [Reporter]SCO3662 (15F20)_PCR/686 [Gene]SCO3662/624 NC_003888 SCH44.02c, unknown, len: 111 aa 2 2 3 11 [Reporter]SCO4510 (11N15)_PCR/2574 [Gene]SCO4510/2342 NC_003888 SCD35.17c, unknown, len: 135 aa 2 1 9 6 [Reporter]SCO2951 (16B20)_PCR/685 [Gene]SCO2951/623 NC_003888 SCE59.10c, probable malate oxidoreductase, len: 471 aa; similar to SW:MAOX_BACST (EMBL:M19485) Bacillus stearothermophilus NAD-dependent malic enzyme (EC 1.1.1.38) (NAD-ME) 478 aa; fasta scores: opt: 1778 z-score: 1887.0 E(): 0; 58.8% identity in 471 aa overlap and to SW:MAO2_ECOLI (EMBL:AE000333) Escherichia coli NADP-dependent malic enzyme (EC 1.1.1.40) MaeB, 759 aa; fasta scores: opt: 1092 z-score: 1158.4 E(): 0; 46.4% identity in 394 aa overlap Contains two Pfam matches to entry PF00390 malic, Malic enzyme 2 2 3 10 [Reporter]SCO3294 (12J15)_PCR/2573 [Gene]SCO3294/2341 NC_003888 SCE15.11c, possible transferase, len: 448aa; weak similarity to SW:MTRD_METMA N5-methyltetrahydromethanopterin coenzyme M methyltransferase from Methanosarcina mazei (250 aa) fasta scores; opt: 126, z-score: 151.3, E(): 0.41, (26.3% identity in 137 aa overlap). Contains TTA leucine codon in the N-terminal region - possible target for bldA regulation. 2 1 9 5 [Reporter]SCO0851 (17N16)_PCR/684 [Gene]SCO0851/622 NC_003888 SCM2.04c, possible PfkB-family carbohydrate kinase, len: 354 aa. Similar to many carbohydrate kinases including Bacillus subtilis SW:KDGK_BACSU(EMBL:L47838) 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45) KdgK (324 aa), fasta scores opt: 497 z-score: 554.8 E(): 1.6e-23 35.0% identity in 311 aa overlap and Bacillus stearothermophilus TR:Q9ZFM1(EMBL:AF098273) 2-keto-3-deoxy-gluconate kinase (314 aa), fasta scores opt: 541 z-score: 603.5 E(): 3.1e-26 36.7% identity in 308 aa overlap. Contains a Pfam match to entry PF00294 pfkB, pfkB family carbohydrate kinase. 2 2 3 9 [Reporter]SCO0797 (13F15)_PCR/2572 [Gene]SCO0797/2340 NC_003888 SCF43.08, possible integral membrane protein, len: 350 aa. Contains possible membrane spanning hydrophobic domains and a TTA /leucine codon, possible target for bldA regulation. 2 1 9 4 [Reporter]SCP1.274 (18J16)_PCR/683 [Gene]SCP1.274/621 NC_003888 SCP1.274, possible large secreted protein, len: 2082aa; similar to TR:O86585 (EMBL:AL031514) hypothetical protein SC2H4.02 from Streptomyces coelicolor (2183 aa) fasta scores: E(): 0, 38.4% id in 1958 aa. Contains a possible cleavable N-terminal signal sequence. 2 1 9 3 [Reporter]SCO1669 (19F16)_PCR/682 [Gene]SCO1669/620 NC_003888 SCI52.11c, possible ATP/GTP-binding protein, len: 351 aa; similar to TR:O53565 (EMBL:AL022022) Mycobacterium tuberculosis hypothetical 37.6 kDa protein MTV023.27c, 347 aa; fasta scores: opt: 859 Z-score: 986.1 bits: 190.9 E(): 2.4e-47; 41.449% identity in 345 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 3 20 2 [Reporter]SCO3060 (20J22)_PCR/4955 [Gene]SCO3060/4519 NC_003888 SCBAC19G2.15, purK, phosphoribosylaminoimidazole carboxylase ATPase subunit PurK, len: >110 aa: similar to many eg. SW:Q44678 (PURK_CORAM) phosphoribosylaminoimidazole carboxylase ATPase subunit PurK from Corynebacterium ammoniagenes (413 aa) fasta scores; opt: 324, Z-score: 401.4, 47.170% identity (48.077% ungapped) in 106 aa overlap.,SCE25.01c, purK, phosphoribosylaminoimidazole carboxylase ATPase subunit PurK, len: >368 aa; highly similar to SW:PURK_CORAM (EMBL:X91189) Corynebacterium ammoniagenes phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21) PurK, 413 aa; fasta scores: opt: 934 z-score: 1056.6 E(): 0; 48.2% identity in 326 aa overlap 2 3 19 22 [Reporter]SCO1337 (1J18)_PCR/4953 [Gene]SCO1337/4518 NC_003888 2SCG61.19c, possible oxidoreductase, len: 301 aa; similar to SW:P37168 (MVIM_SALTY) virulence factor MviM from Salmonella typhimurium (307 aa) fasta scores; opt: 467, z-score: 533.5, E(): 3.1e-22, 33.9% identity in 304 aa overlap. Contains Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family. 2 3 19 21 [Reporter]SCO5737 (2F18)_PCR/4952 [Gene]SCO5737/4517 NC_003888 SC3C3.23, gpsI, guanosine pentaphosphate synthetase/polyribonucleotide nucleotidyltransferase, partial CDS, len: >716 aa; almost identical to Streptomyces antibioticus GpsI TR:Q53597 (EMBL:U19858) guanosine pentaphosphate synthetase (740 aa), 93.6% identity in 715 aa overlap. Contains Pfam match to entry PF00013 KH-domain, KH domain family of RNA binding proteins, score 27.40, E-value 0.00033 and Pfam match to entry PF00575 S1, S1 RNA binding LACR_STAAU motif, score 28.80, E-value 0.00013,SC9A10.01, gpsI, guanosine pentaphosphate synthetase/polyribonucleotide nucleotidyltransferase, partial CDS, len: >57 aa; almost identical to Streptomyces antibioticus GpsI TR:Q53597 (EMBL:U19858) guanosine pentaphosphate synthetase (740 aa), 87.9% identity in 58 aa overlap. Overlaps and extends SC3C3.23. 2 3 19 20 [Reporter]SCO7646 (3B18)_PCR/4951 [Gene]SCO7646/4516 NC_003888 SC10F4.19, possible membrane protein, len: 90 aa; similar to TR:O86580 (EMBL:AL031184) Streptomyces coelicolor small hydrophobic protein SC2A11.19, 97 aa; fasta scores: opt: 181 z-score: 235.5 E(): 1.4e-05; 44.0% identity in 91 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 19 19 [Reporter]SCO7211 (4N14)_PCR/4950 [Gene]SCO7211/4515 NC_003888 SC2H12.10c, possible secreted glycosyl hydrolase, len: 579 aa. Similar in regions to many glycosyl hydrolases including: Arthrobacter sp. SW:E13B_ARTSP(EMBL:D23668) glucan endo-1,3-beta-glucosidase precursor (EC 3.2.1.39) (548 aa), fasta scores opt: 449 z-score: 497.8 E(): 3e-20 27.8% identity in 533 aa overlap. Also similar to the C-terminus of Streptomyces coelicolor TR:O69947(EMBL:AL023862) hypothetical 133.5 kd protein, SC3F9.07 (1238 aa), fasta scores opt: 1923 z-score: 2123.6 E(): 0 66.5% identity in 403 aa overlap. Note, the last 131 aa of this CDS are identical to those in the analogous position on the adjoining CDS, SC2H12.11c. Contains a possible N-terminal signal sequence. Contains a Pfam match to entry PF00652 Ricin_B_lectin, Similarity to lectin domain of ricin beta-chain, 3 copies. 2 3 19 16 [Reporter]SCO5638 (7B14)_PCR/4947 [Gene]SCO5638/4514 NC_003888 SC6A9.29, probable integral membrane protein, len: 295 aa; this ORF has atypically low GC bias in FramePlot 2 3 19 15 [Reporter]SCO6010 (8N10)_PCR/4946 [Gene]SCO6010/4513 NC_003888 SC7B7.07, probable ABC-transport system ATP binding protein, len: 260 aa; similar to many eg. XYLG_ECOLI P37388 d-xylose transport atp-binding protein (513 aa), fasta scores; opt: 567 z-score: 580.0 E(): 3.8e-25, 36.5% identity in 255 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Possible alternative start 9 bp upstream. 2 2 3 8 [Reporter]SCO2877 (14B15)_PCR/2571 [Gene]SCO2877/2339 NC_003888 SCE6.14, possible membrane protein, len: 128 aa. Contains an N-terminal membrane spanning hydrophobic domain. 2 3 19 14 [Reporter]SCO7001 (9J10)_PCR/4945 [Gene]SCO7001/4512 NC_003888 SC8F11.27c, possible integral membrane protein, len: 353 aa. Similar to many proteins of undefined function including: Methanococcus jannaschii SW:Y170_METJA(EMBL:U67474) (350 aa), fasta scores opt: 410 z-score: 451.3 E(): 1e-17 26.3% identity in 335 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence. Also contains a Pfam match to entry PF00924 UPF0003, Uncharacterized protein family UPF0003 2 2 3 7 [Reporter]SCO5154 (15N11)_PCR/2570 [Gene]SCO5154/2338 NC_003888 SCP8.17c, hypothetical protein, len: 433 aa; highly similar to TR:O86315 (EMBL:Z98260) Mycobacterium tuberculosis hypothetical 47.4 kD protein MTV006.04c, 435 aa; fasta scores: opt: 1335 z-score: 1494.5 E(): 0; 49.9% identity in 425 aa overlap and C-terminal region similar to C-terminal region of TR:Q9X0P4 (EMBL:AE001773) Thermotoga maritima Mg2+ transporter MgtE, putative, 446 aa; fasta scores: opt: 390 z-score: 439.1 E(): 5.4e-17; 31.3% identity in 240 aa overlap 2 3 19 13 [Reporter]SCO7372 (10F10)_PCR/4944 [Gene]SCO7372/4511 NC_003888 SC9H11.26c, unknown, len: 177 aa. 2 1 9 2 [Reporter]SCO0984 (20N12)_PCR/681 [Gene]SCO0984/619 NC_003888 SCBAC19F3.11, possible 3-hydroxyacyl-CoA dehydrogenase, len: 289 aa; similar to many e.g. SW:P52041 (HBD_CLOAB) 3-hydroxybutyryl-CoA dehydrogenase from Clostridium acetobutylicum (282 aa) fasta scores; opt: 697, Z-score: 765.4, 40.702% identity (41.281% ungapped) in 285 aa overlap and TR:O88051 (EMBL:AL031541) putative 3-hydroxyacyl-CoA dehydrogenase SCI35.13 from Streptomyces coelicolor (303 aa) fasta scores; opt: 1134, Z-score: 1238.6, 61.538% identity (61.538% ungapped) in 286 aa overlap. Contains Pfam match to entry PF02737 3HCDH_N, 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain, Pfam match to entry PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) 2 2 3 6 [Reporter]SCO0951 (16J11)_PCR/2569 [Gene]SCO0951/2337 NC_003888 SCM11.06c, probable transport system permease protein, len: 310 aa; similar to TR:P73352 (EMBL:D90905) Synechocystis sp. lactose transport system permease protein LacF, 298 aa; fasta scores: opt: 750 z-score: 885.7 E(): 0; 44.9% identity in 292 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and to prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 2 3 19 12 [Reporter]SCO2621 (11B10)_PCR/4943 [Gene]SCO2621/4510 NC_003888 SCC80.06c, putative membarne protein, len: 64 aa; similar to TR:Q9RJX5 (EMBL:AL133210) Streptomyces coelicolor hypothetical 6.6 kDa protein SCG11A.26c, 64 aa; fasta scores: opt: 175 z-score: 234.0 E(): 1.6e-05; 46.0% identity in 63 aa overlap. Contains possible hydrophobic membrane spanning regions 2 1 8 22 [Reporter]SCO1237 (1N8)_PCR/679 [Gene]SCO1237/618 NC_003888 2SCG1.12c, hypothetical protein, len: 94 aa; similar to TR:O50462 (EMBL:AL021006) Mycobacterium tuberculosis hypothetical 9.8 kD protein MTV006.19c, 89 aa; fasta scores: opt: 141 z-score: 195.0 E(): 0.0021; 36.6% identity in 71 aa ovelap 2 2 3 5 [Reporter]SCO1041 (17F11)_PCR/2568 [Gene]SCO1041/2336 NC_003888 SCG20A.21, hypothetical protein, len: 407 aa; similar to SW:YGJO_ECOLI (EMBL:U18997) Escherichia coli hypothetical 43.4 kD protein in EbgC-UxaA intergenic region Ygjo, 388 aa; fasta scores: opt: 956 z-score: 1103.1 E(): 0; 44.0% identity in 373 aa overlap 2 1 8 21 [Reporter]SCO0188 (2J8)_PCR/678 [Gene]SCO0188/617 NC_003888 SCJ1.37, crtV, probable methylesterase, len: 336 aa. Highly similar to Streptomyces griseus TR:P72448 (EMBL; X95596) methylesterase (338 aa), fasta scores opt: 1315 z-score: 1538.7 E(): 0 60.5% identity in 337 aa overlap 2 2 3 4 [Reporter]SCO3774 (18B11)_PCR/2567 [Gene]SCO3774/2335 NC_003888 SCH63.21, possible beta-lactamase related protein, len: 292 aa; similar to SW:BLAB_STRCI (EMBL:D00937) Streptomyces cacaoi beta-lactamase regulatory protein BlaB, 312 aa; fasta scores: opt: 225 z-score: 271.2 E(): 1.4e-07; 34.6% identity in 301 aa overlap 2 1 8 20 [Reporter]SCO0782 (3F8)_PCR/677 [Gene]SCO0782/616 NC_003888 3SCF60.14c, prsA, ribose-phosphate pyrophosphokinase, len: 317 aa; similar to SW:KPRS_SYNP7 (EMBL:D14994) Synechococcus sp. ribose-phosphate pyrophosphokinase (EC 2.7.6.1) PrsA, 331 aa; fasta scores: opt: 795 z-score: 931.4 E(): 0; 42.7% identity in 307 aa overlap. Contains Pfam match to entry PF00156 Pribosyltran, Phosphoribosyl transferase domain 2 2 3 3 [Reporter]SCP1.164c (19N7)_PCR/2566 [Gene]SCP1.164c/2334 NC_003888 SCP1.164c, unknown, len: 130aa; similar to TR:O68053 (EMBL:AF010496) hypothetical protein from Rhodobacter capsulatus (139 aa) fasta scores; opt: 223, z-score: 291.8, E(): 8.7e-09, 38.9% identity in 126 aa overlap. 2 1 8 19 [Reporter]SCO5527 (4B8)_PCR/676 [Gene]SCO5527/615 NC_003888 SC1C2.08, unknown, len: 339 aa; similar to two hypothetical proteins TR:O41120 (EMBL:U42580) A638R protein from Paramecium bursaria chlorella virus 1 (360 aa), fasta scores; opt: 635 z-score: 688.3 E(): 4.4e-31, 35.0% identity in 343 aa overlapTR:O24890 (EMBL:AE000526) HP0049 from Helicobacter pylori (330 aa)opt: 416 z-score: 316.9 E(): 2.1e-10, 25.5% identity in 337 aa overlap 2 2 3 2 [Reporter]SCP1.204c (20F7)_PCR/2565 [Gene]SCP1.204c/2333 NC_003888 SCP1.204c, unknown, len: 553aa; 2 1 8 18 [Reporter]SCO6569 (5N4)_PCR/675 [Gene]SCO6569/614 NC_003888 SC3F9.04, probable secreted solute binding protein, len: 348 aa; similar to many e.g. RBSB_HAEIN D-ribose-binding periplasmic protein precursor (292 aa), fasta scores; opt: 341 z-score: 284.7 E(): 1.2e-08, 29.2% identity in 271 aa overlap. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 2 2 2 22 [Reporter]SCO5198 (1F3)_PCR/2563 [Gene]SCO5198/2332 NC_003888 2SC3B6.22c, hypothetical protein, len: 171 aa; low similarity to TR:Q9S2D5 (EMBL:AL110470) Streptomyces coelicolor MutT domain containing protein, 204 aa; fasta scores: opt: 139 z-score: 184.0 E(): 0.0088; 30.8% identity in 120 aa overlap 2 1 8 17 [Reporter]SCO6672 (6J4)_PCR/674 [Gene]SCO6672/613 NC_003888 SC5A7.22, unknown, len: 295 aa; similar to a hypothetical protein from M. tuberculosis TR:O33337 (EMBL:AL008967) MTV002.60C (324 aa), fasta scores; opt: 1202 z-score: 1507.9 E(): 0, 57.2% identity in 285 aa overlap 2 2 2 21 [Reporter]SCO0737 (2B3)_PCR/2562 [Gene]SCO0737/2331 NC_003888 3SC5B7.15c, probable oxidoreductase, len: 266 aa; similar to SW:FABG_HAEIN (EMBL:U32701) Haemophilus influenzae 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) FabG, 242 aa; fasta scores: opt: 510 z-score: 584.5 E(): 4.9e-25; 41.4% identity in 239 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus 2 1 8 16 [Reporter]SCO7289 (7F4)_PCR/673 [Gene]SCO7289/612 NC_003888 SC5H1.03, possible regulator, len: 142 aa; similar to TR:P95753 (EMBL:D50051), ssgA, Streptomyces griseus gene involved in regulation of sporulation and cell division (136 aa), fasta scores; opt: 175 z-score: 240.4 E(): 4.6e-06, 32.5% identity in 120 aa overlap. Similar to SC5F2A.05c (EMBL:AL049587) S.coelicolor possible regulator (138 aa) (33.8% identity in 145 aa overlap) 2 2 2 20 [Reporter]SCO4600 (2N23)_PCR/2561 [Gene]SCO4600/2330 NC_003888 SCD20.18, nuoB2, NADH dehydrogenase subunit, len: 232 aa; similar to SW:NUKM_BRAOL (EMBL:X82274) Brassica oleracea NADH-ubiquinone oxidoreductase 20 kDa subunit precursor (EC 1.6.5.3) NukM, 215 aa; fasta scores: opt: 626 z-score: 616.8 E(): 6.9e-27; 52.4% identity in 166 aa overlap, to SW:NUOB_ECOLI (EMBL:X68301) Escherichia coli NADH dehydrogenase I chain B (EC 1.6.5.3) NuoB, 220 aa; fasta scores: opt: 583 z-score: 575.3 E(): 1.4e-24; 58.1% identity in 136 aa overlap and to TR:Q9XAQ5 (EMBL:AL07861) Streptomyces coelicolor SCD16A.20c, NuoB, NADH dehydrogenase subunit, 184 aa; fasta scores: opt: 531 z-score: 455.5 E(): 9.8e-20; 50.7% identity in 136 aa overlap. Contains Pfam match to entry PF01058 oxidored_q6, NADH ubiquinone oxidoreductase, 20 Kd subunit and possible hydrophobic membrane spanning region 2 1 8 15 [Reporter]SCO6687 (8B4)_PCR/672 [Gene]SCO6687/611 NC_003888 SC6G3.03, hypothetical protein, len: 1678 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains probable helix-turn-helix motif at aa 1209-1230 (Score 1004, +2.61 SD). Similar to TR:Q9RJY8 (EMBL:AL133210) Streptomyces coelicolor hypothetical protein SCF37.13, 1726 aa; fasta scores: opt: 690 Z-score: 706.5 E(): 1e-31; 35.556% identity in 1800 aa overlap 2 1 8 13 [Reporter]SCO6467 (9J24)_PCR/670 [Gene]SCO6467/610 NC_003888 SC9C7.03c, probable integral membrane protein, possible phosphatidylserine synthase, len: 260 aa; similar to many from prokaryotes (all putative) and eukaryotes eg. SW:PSS_YEAST phosphatidylserine synthase from Saccharomyces cerevisiae (275 aa) fasta scores; opt: 187, z-score: 254.6, E(): 6.8e-07, (35.9% identity in 170 aa overlap). Contains possible membrane spanning regions 2 3 19 11 [Reporter]SCO3956 (12N6)_PCR/4942 [Gene]SCO3956/4509 NC_003888 SCD78.23, putative ABC transporter ATP-binding protein, similar to YHCH_BACSU (EMBL:X96983) hypothetical ABC transporter ATP-binding protein from Bacillus subtilis, fasta scores; opt: 438, z-score: 522.7, E(): 7.2e-22, (33.6% identity in 220 aa overlap) and YHIH_ECOLI (EMBL:U00039) ABC transporter ATP-binding protein from Escherichia coli fasta scores; opt: 445, z-score: 530.5, E(): 2.6e-22, (30.7% identity in 287 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporters, score 183.40, E-value 3.8e-51 and PS00017 ATP /GTP-binding site motif A (P-loop). 2 3 19 10 [Reporter]SCO3270 (13J6)_PCR/4941 [Gene]SCO3270/4508 NC_003888 SCE39.20, unknown, len: 170aa; 2 3 19 9 [Reporter]SCO0286 (14F6)_PCR/4940 [Gene]SCO0286/4507 NC_003888 SCF85.14c, possible secreted peptidoglycan binding protein, len: 168 aa. Some similarity to N-acetylmuramoyl-L-alanine amidases over the peptidoglycan binding domains e.g. Bacillus subtilis SW:CWLA_BACSU (EMBL; X51424) (272 aa), fasta scores opt: 127 z-score: 157.1 E():0.21 34.9% identity in 63 aa overlap. Contains a Pfam match to entry PF01471 PG_binding_1, Putative peptidoglycan binding domain. Contains a possible N-terminal signal sequence 2 3 19 8 [Reporter]SCO0122 (15B6)_PCR/4939 [Gene]SCO0122/4506 NC_003888 SCJ21.03, probable flavin-containing monooxygenase (putative secreted protein), len: 458 aa; similar to many eukaryotic monooxygenases, e.g. FMO5_RABIT dimethylaniline monooxygenase [N-oxide forming] (532 aa), fasta scores; opt: 938 z-score: 1016.9 E(): 0, 38.7% identity in 432 aa overlap. Also similar to S. coelicolor putative oxidoreductase SCH35.27 E(): 3.4e-07, 34.8% identity in 282 aa overlap. Contains Pfam match to entry PF00743 FMO-like, Flavin-binding monooxygenase-like 2 3 19 7 [Reporter]SCO3745 (16N2)_PCR/4938 [Gene]SCO3745/4505 NC_003888 SCH22A.23c, possible lipoprotein, len: 163 aa; similar to TR:P96401 (EMBL:Z92669) Mycobacterium tuberculosis hypothetical 20.1 kD protein, 182 aa; fasta scores: opt: 287 z-score: 377.4 E(): 1.3e-13; 38.9% identity in 162 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence. Also contains possible hydrophobic membrane spanning regions 2 3 19 6 [Reporter]SCO4968 (17J2)_PCR/4937 [Gene]SCO4968/4504 NC_003888 2SCK31.28, possible membrane protein, len: 75 aa. Contains possible hydrophobic membrane spanning region 2 3 19 5 [Reporter]SCO3463 (18F2)_PCR/4936 [Gene]SCO3463/4503 NC_003888 SCE46.20, probable phosphorylase, len: 262 aa; similar to TR:O58891 (EMBL:AP000005) Pyrococcus horikoshii 265 aa long hypothetical 5'-methylthioadenosine phosphorylase PH1143 265 aa; fasta scores: opt: 461 z-score: 534.1 E(): 2.1e-22; 35.1% identity in 251 aa overlap and to SW:MTAP_HUMAN (EMBL:U22233) Homo sapiens 5'-methylthioadenosine phosphorylase (EC 2.4.2.28) MtaP, 283 aa; fasta scores: opt: 373 z-score: 433.4 E(): 8.6e-17; 32.4% identity in 216 aa overlap. Contains Pfam match to entry PF00896 Mtap_PNP, phosphorylases family 2 2 2 2 19 [Reporter]SCO6139 (3J23)_PCR/2560 [Gene]SCO6139/2329 NC_003888 SC1A9.03, probable two component sensor kinase, len: 388aa; similar to a family of sensor kinases egs. TR:Q53893 (EMBL:U51332) AbsA1 from Streptomyces coelicolor (571 aa), fasta scores; opt: 389, z-score: 278.9, E(): 2.9e-08, (33.1% identity in 405 aa overlap) and UHPB_ECOLI (EMBL:M17102) sensor kinase from Escherichia coli (500 aa), fasta scores; opt:221, z-score: 248.0, E(): 1.5e-06, (25.9% identity in 398 aa overlap). Contains several possible membrane spanning domains. 2 3 19 4 [Reporter]SCP1.03c (19B2)_PCR/4935 [Gene]SCP1.03c/4502 NC_003888 SCP1.03c, unknown, len: 72aa; 2 2 2 18 [Reporter]SCO6196 (4F23)_PCR/2559 [Gene]SCO6196/2328 NC_003888 SC2G5.17, probable long-chain-fatty-acid-CoA ligase,len: 541aa; similar to many (prokaryote and eukaryote) eg.TR:O06168 (EMBL:Z95556) hypothetical protein from Mycobacterium tuberculosis (547 aa) fasta scores; opt:2202, z-score:2509.4, E(): 0, (61.6% identity in 528 aa overlap) and SW:4CL1_SOLTU 4-coumarate-CoA ligase 1 from Solanum tuberosum (potato) (545 aa) fasta scores; opt: 746, z-score: 850.8, E(): 0, (31.1% identity in 511 aa overlap). Contains PS00455 Putative AMP-binding domain signature and Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme, score 148.80,E-value 9.6e-41. 2 3 19 3 [Reporter]SCO5353 (19N22)_PCR/4934 [Gene]SCO5353/4501 NC_003888 SCBAC5H2.22, lysA, diaminopimelate decarboxylase, len: 463 aa; highly similar to SW:DCDA_CORGL (EMBL:X07563) Corynebacterium glutamicum diaminopimelate decarboxylase (EC 4.1.1.20) LysA, 445 aa; fasta scores: opt: 1503 z-score: 1722.5 E(): 0; 51.0% identity in 443 aa overlap. Contains Pfam match to entry PF00278 Orn_DAP_Arg_deC, Pyridoxal-dependent decarboxylase and matches to Prosite entries PS00878 Orn/DAP/Arg decarboxylases family 2 pyridoxal-P attachment site and PS00879 Orn/DAP/Arg decarboxylases family 2 signature 2 2 1 8 12 [Reporter]SCO2314 (10F24)_PCR/669 [Gene]SCO2314/609 NC_003888 SCC53.05, possible integral membrane protein, len: 289 aa; contains possible membrane-spanning hydrophobic regions. Also contains Gly/Pro/Gln-rich region 160-220aa. 2 2 2 17 [Reporter]SCO7135 (5B23)_PCR/2558 [Gene]SCO7135/2327 NC_003888 SC4B10.36c, unknown, len: 135 aa 2 3 19 2 [Reporter]SCO0982 (20F22)_PCR/4933 [Gene]SCO0982/4500 NC_003888 SCBAC19F3.09, aceA, isocitrate lyase, len: 426 aa: strongly similar to many e.g. SW:O53752 (ACEA_MYCTU) isocitrate lyase from Mycobacterium tuberculosis (428 aa) fasta scores; opt: 2144, Z-score: 2405.5, 74.822% identity (75.000% ungapped) in 421 aa overlap. Contains Pfam matches to entry PF00463 ICL, Isocitrate lyase family and Prosite match to PS00161 Isocitrate lyase signature. 2 1 8 11 [Reporter]SCO4682 (11B24)_PCR/668 [Gene]SCO4682/608 NC_003888 SCD31.07, possible tautomerase, len: 69 aa; similar to TR:O25581 (EMBL:AE000602) Helicobacter pylori 4-oxalocrotonate tautomerase (DmpI) HP0924, 68 aa; fasta scores: opt: 114 z-score: 175.1 E(): 0.025; 32.2% identity in 59 aa overlap. Contains Pfam match to entry PF01361 Tautomerase, Tautomerase enzyme 2 2 2 16 [Reporter]SCO7300 (6N19)_PCR/2557 [Gene]SCO7300/2326 NC_003888 SC5F8.10c, unknown, len: 410 aa. 2 1 8 10 [Reporter]SCO3523 (12N20)_PCR/667 [Gene]SCO3523/607 NC_003888 SCE2.04, unknown, len: 241aa; 2 2 2 15 [Reporter]SCO4411 (7J19)_PCR/2556 [Gene]SCO4411/2325 NC_003888 SC6F11.09, probable calcium binding protein, len: 170 aa; similar to SW:CBP_SACER (EMBL:M29700) Saccharopolyspora erythraea calerythrin (calcium-binding protein), 177 aa; fasta scores: opt: 229 z-score: 267.7 E(): 2.1e-07; 28.6% identity in 154 aa overlap. Contains 4x Pfam matches to entry PF00036 efhand, EF hand and 3x matches to Prosite entry PS00018 EF-hand calcium-binding domain 2 1 8 9 [Reporter]SCO2880 (13J20)_PCR/666 [Gene]SCO2880/606 NC_003888 SCE6.17, cvnB12, unknown, len: 140 aa. Similar to many proteins of undefined function from Streptomyces coelicolor e.g. TR:Q9X835 (EMBL:AL049727) SC9B1.15C (139 aa), fasta scores opt: 222 z-score: 297.5 E(): 3.9e-09 34.3% identity in 134 aa overlap and TR:CAB61281 (EMBL:AL132991) SCF55.11C (152 aa), fasta scores opt: 221 z-score: 295.6 E(): 4.9e-09 32.6% identity in 138 aa overlap. Contains a Pfam match to entry PF01860 DUF42, Protein of unknown function. 2 2 2 14 [Reporter]SCO6874 (8F19)_PCR/2555 [Gene]SCO6874/2324 NC_003888 SC7F9.26c, unknown, len: 211 aa. 2 1 8 8 [Reporter]SCO3171 (14F20)_PCR/665 [Gene]SCO3171/605 NC_003888 SCE87.22c, possible hydrolase, len: 314 aa: Similar to several e.g. Mycobacterium tuberculosis TR:O53321 (EMBL: AL021646) hypothetical 32.1 KD protein (299 aa), fasta scores opt: 261 z-score: 297.3 E(): 3.5e-09 31.7% identity in 281 aa overlap and Streptomyces lividans SW:PRXC_STRLI (EMBL: U02635) non-heme chloroperoxidase (EC 1.11.1.10) (275 aa), fasta scores opt: 241 z-score: 275.2 E(): 5.9e-08 30.0% identity in 287 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 2 2 2 13 [Reporter]SCO7150 (9B19)_PCR/2554 [Gene]SCO7150/2323 NC_003888 SC9A4.12c, possible acetyltransferase, len: 278 aa; similar to TR:Q9V2R3 (EMBL:AJ248283) Pyrococcus abyssi hypothetical 30.2 kDa protein PAB2346, 266 aa; fasta scores: opt: 149 z-score: 182.6 E(): 0.011; 33.3% identity in 90 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 2 1 8 7 [Reporter]SCO1840 (15B20)_PCR/664 [Gene]SCO1840/604 NC_003888 SCI8.25c, probable ABC transporter, ATP binding subunit, len: 532 aa. Highly similar to many e.g. Mycobacterium tuberculosis TR:O53164 (EMBL: AL021184) ABC transporter ATP binding protein (542 aa), fasta scores opt: 2213 z-score: 2308.8 E():0 63.5% identity in 542 aa overlap and Escherichia coli SW: YBIT_ECOLI (EMBL: AE000184) ABC transporter ATP-binding protein (530 aa), fasta scores opt: 921 z-score: 963.6 E():0 33.5% identity in 534 aa overlap. Contains 2xPfam match to entry PF00005 ABC_tran ABC transporter. In addition to Prosite hits to 2xPS00017 ATP/GTP-binding site motif A (P-loop) and 2xPS00211 ABC transporters family signature. Also contains a probable coiled-coil from 250 to 286 (37 residues). 2 2 2 12 [Reporter]SCO2339 (10N15)_PCR/2553 [Gene]SCO2339/2322 NC_003888 SCC53.30c, unknown, len: 160aa; 2 1 8 6 [Reporter]SCO4953 (16N16)_PCR/663 [Gene]SCO4953/603 NC_003888 2SCK31.13, unknown, len: 64 aa 2 2 2 11 [Reporter]SCO4506 (11J15)_PCR/2552 [Gene]SCO4506/2321 NC_003888 SCD35.13, conserved hypothetical protein, len: 282 aa; similar to various hypothetical proteins e.g. TR:P94301 (EMBL:U61168) Bacillus firmus hypothetical 32.2 kD protein, 281 aa; fasta scores: opt: 398 z-score: 495.8 E(): 3.5e-20; 31.1% identity in 244 aa overlap 2 1 8 5 [Reporter]SCO3773 (17J16)_PCR/662 [Gene]SCO3773/602 NC_003888 SCH63.20, probable lysR family transcriptional regulatory protein, len: 320 aa; similar to SW:ALSR_BACSU (EMBL:L04470) Bacillus subtilis Als operon regulatory protein AlsR, 302 aa; fasta scores: opt: 463 z-score: 517.7 E(): 3e-21; 30.4% identity in 260 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Also contains possible helix-turn-helix motif at residues 17..38 (+2.87 SD) 2 2 2 10 [Reporter]SCO4253 (12F15)_PCR/2551 [Gene]SCO4253/2320 NC_003888 SCD8A.26c, conserved hypothetical protein, len: 534 aa; similar to TR:Q988X8 (EMBL:AP003009) Rhizobium loti MLR6548 protein, 528 aa; fasta scores: opt: 1186 Z-score: 1203.4 E(): 2.1e-59; 39.963% identity in 538 aa overlap 2 1 8 4 [Reporter]SCO0030 (18F16)_PCR/661 [Gene]SCO0030/601 NC_003888 SCJ4.11, unknown, len: 206 aa; similar in its N-terminal region to the SCP1 plasmid borne TR:Q9ACQ7 (EMBL:AL590464) Streptomyces coelicolor putative secreted protein SCP1.261c, 167 aa; fasta scores: opt: 478 Z-score: 480.3 E(): 4e-19; 74.766% identity in 107 aa overlap 2 1 8 3 [Reporter]SCP1.319c (19B16)_PCR/660 [Gene]SCP1.319c/600 NC_003888 SCP1.319c, unknown, len: 99aa; 2 2 2 9 [Reporter]SCO1778 (13B15)_PCR/2550 [Gene]SCO1778/2319 NC_003888 SCI51.18, possible transcriptional regulator, len: 548 aa; shows weak similarity to TR:Q00509 (EMBL:X63451), SmrR, Streptomyces ambofaciens regulatory gene governing the expression of a polyketide synthase (604 aa), fasta scores; opt: 205 z-score: 220.9 E(): 5.9e-05, 26.5% identity in 460 aa overlap. Also similar to TR:O69807 (EMBL:AL023496) Streptomyces coelicolor hypothetical protein (537 aa) (32.4% identity in 547 aa overlap) and PkwR (EMBL:AF115313) Thermomonospora curvata putative regulatory protein (535 aa) (28.8% identity in 534 aa overlap). Contains probable helix-turn-helix motif at aa 498-519 (Score 1712 (+5.02 SD) 2 2 2 8 [Reporter]SCO0749 (14N11)_PCR/2549 [Gene]SCO0749/2318 NC_003888 SCF81.08c, probable dehydratase, len: 324 aa; higly similar to TR:AAD55454 (EMBL:AF055579) Streptomyces antibioticus dehydratase OleE, 335 aa; fasta scores: opt: 1571 z-score: 1848.7 E(): 0; 74.0% identity in 323 aa overlap and to SW:STRE_STRGR (EMBL:X62567) Streptomyces griseus DTDP-glucose 4,6-dehydratase (EC 4.2.1.46) StrE, 328 aa; fasta scores: opt: 1422 z-score: 1674.0 E(): 0; 68.6% identity in 318 aa overlap. Contains match to Pfam entry PF01370 Epimerase, NAD dependent epimerase/dehydratase family and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 2 2 2 7 [Reporter]SCO3644 (15J11)_PCR/2548 [Gene]SCO3644/2317 NC_003888 SCH10.22c, possible lipase/esterase, len: 301 aa; weakly similar to many esterases e.g. SW:EST1_CAEEL (EMBL:M96145) Caenorhabditis elegans gut esterase precursor (562 aa), fasta scores; opt: 150 z-score: 166.4 E(): 0.059, 29.3% identity in 280 aa overlap. Contains PS00120 Lipases, serine active site 2 2 2 6 [Reporter]SCO1060 (16F11)_PCR/2547 [Gene]SCO1060/2316 NC_003888 SCG22.06, probable transcriptional repressor, len: 413 aa; similar to TR:O05180 (EMBL:Z71474) Bacillus megaterium Xyl repressor XylR, 388 aa; fasta scores: opt: 382 z-score: 417.8 E(): 8.5e-16; 31.3% identity in 386 aa overlap and to TR:CAB61583 (EMBL:AL133210) Streptomyces coelicolor possible xylose repressor SCF37.02, 402 aa; fasta scores: opt: 586 z-score: 523.0 E(): 1.5e-23; 48.0% identity in 396 aa overlap. Contains Pfam match to entry PF00480 ROK, ROK family and a possible helix-turn-helix motif at residues 35..56 (+3.03 SD) 2 2 2 5 [Reporter]SCO2867 (17B11)_PCR/2546 [Gene]SCO2867/2315 NC_003888 SCE6.04, unknown, len: 75 aa. Similar to another hypothetical protein from Streptomyces coelicolor TR:CAB53305 (EMBL:AL109973) SCJ30.10C (92 aa), fasta scores opt: 101 z-score: 154.5 E(): 0.35 32.1% identity in 81 aa overlap. 2 2 2 4 [Reporter]SCO1162 (18N7)_PCR/2545 [Gene]SCO1162/2314 NC_003888 SCG8A.16, possible methyltransferase, len: 199 aa; similar to TR:Q55214 (EMBL:L35154) aklanonic acid methyltransferase from Streptomyces sp. (220 aa) fasta scores; opt: 228, z-score: 264.1, E(): 2.9e-07, 34.1% identity in 123 aa overlap. 2 2 2 3 [Reporter]SCP1.135 (19J7)_PCR/2544 [Gene]SCP1.135/2313 NC_003888 SCP1.135, unknown, len: 313aa; 2 2 2 2 [Reporter]SCP1.183 (20B7)_PCR/2543 [Gene]SCP1.183/2312 NC_003888 SCP1.183, unknown, len: 180aa; 2 2 1 22 [Reporter]SCO5194 (1B3)_PCR/2541 [Gene]SCO5194/2311 NC_003888 2SC3B6.18, hypothetical protein, len: 168 aa; similar to TR:CAB72040 (EMBL:AJ391262) Neisseria meningitidis hypothetical 26.6 kD protein RNI5, 230 aa; fasta scores: opt: 249 z-score: 315.5 E(): 4.2e-10; 41.7% identity in 103 aa overlap 2 2 1 21 [Reporter]SCO4927 (1N23)_PCR/2540 [Gene]SCO4927/2310 NC_003888 SCK13.19, probable ligase, len: 291 aa; similar to C-terminal domain of SW:BIRA_ECOLI (EMBL:M10123) Escherichia coli BirA bifunctional protein [includes: biotin operon repressor biotin--[acetyl-CoA-carboxylase] synthetase (EC 6.3.4.1) (biotin--protein ligase)], 321 aa; fasta scores: opt: 340 z-score: 382.0 E(): 9.7e-14; 32.7% identity in 260 aa overlap. Contains Pfam match to entry PF01317 BPL, Biotin protein ligase catalytic domain and PF02237 BPL_C, Biotin protein ligase C terminal domain 2 2 1 20 [Reporter]SCO5227 (2J23)_PCR/2539 [Gene]SCO5227/2309 NC_003888 SC7E4.24c, nrdX, putative redoxin, len: 91 aa; identical to previously sequenced TR:CAB82484 (EMBL:AJ276618) Streptomyces coelicolor A3(2) putative redoxin NrdX, 91 aa. Doubtful CDS: Frameplot and Correlation Scores indicate that this CDS might not be a gene 2 2 1 19 [Reporter]SCO7402 (3F23)_PCR/2538 [Gene]SCO7402/2308 NC_003888 SC10G8.30c, possible lyase, len: 390 aa. Weakly similar to Bacillus subtilis SW:SPL_BACSU(EMBL:L08809) spore photoproduct lyase (EC 4.1.99.-), SplB (342 aa), fasta scores opt: 285 z-score: 328.4 E(): 7.1e-11 26.8% identity in 347 aa overlap. 2 2 1 18 [Reporter]SCO6192 (4B23)_PCR/2537 [Gene]SCO6192/2307 NC_003888 SC2G5.13, hypothetical protein, len: 304 aa; similar to TR:Q9KZ00 (EMBL:AL355753) Streptomyces coelicolor hypothetical protein SC8G12.05, 366 aa; fasta scores: opt: 688 Z-score: 744.6 E(): 7.7e-34; 44.110% identity in 365 aa overlap 2 2 1 17 [Reporter]SCO6717 (5N19)_PCR/2536 [Gene]SCO6717/2306 NC_003888 SC4C6.27c, probable acyl-[acyl-carrier protein] desaturase, len: 328 aa; similar to many e.g. SW:STAD_LINUS (EMBL:X70962) Linum usitatissimum stearoyl-ACP desaturase (396 aa), fasta scores; opt: 385 z-score: 461.5 E(): 2.2e-18, 30.2% identity in 311 aa overlap. The N-terminal 100 aa are divergent. Highest similarity to TR:Q50050 (EMBL:U15182), aadX, Mycobacterium leprae protein (338 aa) (45.5% identity in 325 aa overlap) and TR:Q50824 (EMBL:AL022004), des, Mycobacterium tuberculosis protein (338 aa) (46.1% identity in 323 aa overlap). Contains TTA (leucine) codon, possible target for bldA regulation 2 2 1 16 [Reporter]SCO7296 (6J19)_PCR/2535 [Gene]SCO7296/2305 NC_003888 SC5F8.06c, unknown, len: 117 aa. Similar in parts to Streptomyces coelicolor TR:Q9R3I9(EMBL:AJ249450) hypothetical 9.6 kd protein (86 aa), fasta scores opt: 295 z-score: 318.5 E(): 2.8e-10 51.4% identity in 72 aa overlap. 2 2 1 15 [Reporter]SCO7405 (7F19)_PCR/2534 [Gene]SCO7405/2304 NC_003888 SC6D11.01c, possible acetyltransferase, partial CDS, len: > 72 aa. Similar to the N-terminus of Escherichia coli SW:THGA_ECOLI(EMBL:J01636) galactoside O-acetyltransferase (EC 2.3.1.18) (203 aa), fasta scores opt: 138 z-score: 187.9 E(): 0.0048 38.3% identity in 60 aa overlap. Overlaps and extends into CDS SC10G8.33c on the adjoining cosmid.,SC10G8.33c, possible acetyltransferase, partial CDS, len: > 175 aa. Highly similar to a number of acetyltransferases e.g. Escherichia coli SW:THGA_ECOLI(EMBL:J01636) galactoside o-acetyltransferase (EC 2.3.1.18) LacA (203 aa), fasta scores opt: 592 z-score: 679.4 E(): 2e-30 48.8% identity in 160 aa overlap. Contains a Prosite hit to PS00101 Hexapeptide-repeat containing-transferases signature and a Pfam match to entry PF00132 hexapep, Bacterial transferase hexapeptide (four repeats). 2 2 1 14 [Reporter]SCO6870 (8B19)_PCR/2533 [Gene]SCO6870/2303 NC_003888 SC7F9.22c, unknown, len: 205 aa. 2 2 1 12 [Reporter]SCO2335 (10J15)_PCR/2531 [Gene]SCO2335/2302 NC_003888 SCC53.26c, possible integral membrane protein, len: 761 aa; contains possible membrane-spanning hydrophobic regions. Regions similar to many eg. TR:CAB90881 (EMBL:AL355752) putative integral membrane protein from Streptomyces coelicolor (287 aa) fasta scores; opt: 627, z-score: 671.4, E(): 6.3e-30, 41.0% identity in 290 aa overlap 2 2 1 11 [Reporter]SCO4111 (11F15)_PCR/2530 [Gene]SCO4111/2301 NC_003888 SCD17A.03c, conserved hypothetical protein, len: 271 aa; similar to TR:CAB84769 (EMBL:AL162756) Neisseria meningitidis conserved hypothetical protein NMA1542, 238 aa; fasta scores: opt: 716 z-score: 847.7 E(): 0; 49.5% identity in 222 aa overlap and to SW:MT04_ECOLI (EMBL:U28377) Escherichia coli hypothetical methyltransferase (EC 2.1.1.*) YggH or B2960, 239 aa; fasta scores: opt: 679 Z-score: 793.9 E(): 1.4e-36; 43.590% identity in 234 aa overlap 2 2 1 9 [Reporter]SCO3448 (13N11)_PCR/2528 [Gene]SCO3448/2300 NC_003888 SCE46.05c, unknown, len: 100 aa 4 4 16 21 [Reporter]SCO1600 (2L13)_PCR/7909 [Gene]SCO1600/7199 NC_003888 SCI35.22c, infC, translation initiation factor IF-3, len: 217 aa; highly similar to many e.g. IF3_ECOLI translation initiation factor IF-3 (180 aa), fasta scores; opt: 566 z-score: 818.0 E(): 0, 51.2% identity in 172 aa overlap. Uses an ATC initiation codon, in common with Myxococcus xanthus. E. coli and B. subtilis infC begin with ATT. Contains PS00017 ATP /GTP-binding site motif A (P-loop) and Pfam match to entry PF00707 IF3, Translation initiation factor IF-3, score 201.40, E-value 1.4e-56 4 4 16 20 [Reporter]SCO2216 (3H13)_PCR/7908 [Gene]SCO2216/7198 NC_003888 SC10B7.11, probable two-component system response regulator, len: 217 aa; similar to TR:Q9X8Q7 (EMBL:AL049754) Streptomyces coelicolor putative two-component system regulator SCH10.18c, 228 aa; fasta scores: opt: 349 z-score: 426.3 E(): 2.3e-16; 34.8% identity in 221 aa overlap and to Streptomyces coelicolor SCC75.27c, 228 aa; fasta scores: opt: 722 z-score: 711.8 E(): 2.9e-34; 54.4% identity in 217 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family 1 2 4 5 [Reporter]SCO0342 (17I11)_PCR/2086 [Gene]SCO0342/1899 NC_003888 SCF41.01c, partial CDS, possible secreted protein, len: >350 aa. Contains a possible N-terminal signal sequence.,SCF12.21c, partial CDS, unknown, len: >533aa; 4 4 16 19 [Reporter]SCO5475 (4D13)_PCR/7907 [Gene]SCO5475/7197 NC_003888 SC2A11.09, unknown, len: 265 aa 1 2 4 3 [Reporter]SCP1.101 (19A11)_PCR/2084 [Gene]SCP1.101/1898 NC_003888 SCP1.101, possible plasmid transfer protein, len: 137aa; similar to TR:Q06257 (EMBL:X72857) TraB protein from plasmid SCP2 of Streptomyces coelicolor (137 aa) fasta scores; opt: 142, z-score: 180.4, E(): 0.014, 29.4% identity in 136 aa overlap. Contains possible coiled-coil region at 26-39 residues. 4 4 16 18 [Reporter]SCO2089 (5P9)_PCR/7906 [Gene]SCO2089/7196 NC_003888 SC4A10.22c, murE, probable UDP-N-acetylmuramoylalanyl-D-glutamate- 2,6-diaminopimelate ligase, len: 506 aa; previously partially sequenced as TR:Q9Z5V6 (EMBL:AF123319), MurE, Streptomyces coelicolor UDP-N-acetylmuramoylalanyl-D-glutamate- 2,6-diaminopimelate ligase (fragment) (142 aa) and identical to that sequence. Similar to many e.g. SW:MURE_ECOLI (EMBL:X55814), MurE, Escherichia coli UDP-N-acetylmuramoylalanyl-D-glutamate- 2,6-diaminopimelate ligase (494 aa), fasta scores; opt: 889 z-score: 881.7 E(): 0, 37.9% identity in 486 aa overlap and SW:MURE_MYCTU (EMBL:Z95388), MurE, Mycobacterium tuberculosis probable UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelat e ligase (535 aa) (50.3% identity in 519 aa overlap). Shows weak similarity to others from S.coelicolor e.g. SC9B1.07 (EMBL:AL049727) S.coelicolor possible UDP-N-acetylmuramoyl-L-alanine ligase (462 aa) (26.6% identity in 497 aa overlap). Contains Pfam match to entry PF01225 Mur_ligase, Mur ligase family 1 2 4 2 [Reporter]SCO1663 (20I7)_PCR/2083 [Gene]SCO1663/1897 NC_003888 SCI52.05c, probable cysteinyl-tRNA synthetase, len: 409 aa; similar to SW:SYC_ECOLI (EMBL:) Escherichia coli cysteinyl-tRNA synthetase (EC 6.1.1.16) CysS or B0526, 461 aa; fasta scores: opt: 772 Z-score: 861.2 bits: 168.5 E(): 2.2e-40; 36.908% identity in 401 aa overlap. Contains Pfam match to entry PF01406 tRNA-synt_1e, tRNA synthetases class I (C) 4 4 16 17 [Reporter]SCO7457 (6L9)_PCR/7905 [Gene]SCO7457/7195 NC_003888 SC5C11.14c, unknown, len: 132 aa. 1 2 3 22 [Reporter]SCO4187 (1I3)_PCR/2081 [Gene]SCO4187/1896 NC_003888 2SCD46.01c, putative membrane protein, partial CDS, len: >45aa; strongly similar to neighbouring CDS 2SCD46.03c fasta scores; opt: 169, z-score: 236.7, E(): 1.4e-07, 51.1% identity in 45 aa overlap. Hydrophobic. Contains possible hydrophobic membrane spanning regions,SCD66.24c, putative membrane protein (fragment), len: >48 aa. Contains possible hydrophobic membrane spanning region 4 4 16 16 [Reporter]SCO5683 (7H9)_PCR/7904 [Gene]SCO5683/7194 NC_003888 SC5H4.07, probable two-component system sensor kinase, len: 398 aa; similar to TR:Q9X851 (EMBL:AL049630) Streptomyces coelicolor putative two-component sensor kinase SCE126.08c, 401 aa; fasta scores: opt: 581 z-score: 640.5 E(): 3.2e-28; 36.8% identity in 361 aa overlap and to TR:BAA88406 (EMBL:AB016841) Streptomyces thermoviolaceus ChiS protein, 448 aa; fasta scores: opt: 445 z-score: 491.3 E(): 6.6e-20; 34.5% identity in 391 aa overlap. Contains possible hydrophobic membrane spanning regions in N-terminal domain 1 2 3 21 [Reporter]SCO0740 (2E3)_PCR/2080 [Gene]SCO0740/1895 NC_003888 3SC5B7.18c, probable hydrolase, len: 209 aa; identical to previously sequenced TR:Q9ZNB2 (EMBL:AB019513) Streptomyces coelicolor similar to Mycobacterium Tuberculosis hypothetical protein RV2260, 209 aa and similar to SW:GL2M_ARATH (EMBL:U90928) Arabidopsis thaliana hydroxyacylglutathione hydrolase, mitochondrial precursor (EC 3.1.2.6) Glx2-1, 331 aa; fasta scores: opt: 230 z-score: 271.1 E(): 1.4e-07; 30.1% identity in 173 aa overlap. Contains Pfam match to entry PF00753 lactamase_B, Metallo-beta-lactamase superfamily 4 4 16 14 [Reporter]SCO7830 (9P5)_PCR/7902 [Gene]SCO7830/7193 NC_003888 SC8E7.27c, unknown, len: 114 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1X6(EMBL:AL109973) hypothetical 12.2 kd protein, SCJ30.13 (114 aa). 1 2 3 20 [Reporter]SCO2256 (3A3)_PCR/2079 [Gene]SCO2256/1894 NC_003888 SCC75A.02, panB, 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), len: 291 aa. Highly similar to many including: Escherichia coli SW:PANB_ECOLI(EMBL:L17086) 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) (264 aa), fasta scores opt: 711 z-score: 839.4 E(): 0 44.3% identity in 262 aa overlap and Mycobacterium tuberculosis SW:PANB_MYCTU(EMBL:Z70692) 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) (281 aa), fasta scores opt: 1019 z-score: 1199.2 E(): 0 59.1% identity in 269 aa overlap. 4 4 16 13 [Reporter]SCO2790 (10L5)_PCR/7901 [Gene]SCO2790/7192 NC_003888 SCC105.21c, hypothetical protein, len: 175 aa; highly similar to TR:BAA86264 (EMBL:AB023785) Streptomyces griseus ORF2, 173 aa; fasta scores: opt: 998 z-score: 1157.6 E(): 0; 82.9% identity in 175 aa overlap 1 2 3 19 [Reporter]SCO6142 (3M23)_PCR/2078 [Gene]SCO6142/1893 NC_003888 SC1A9.06, unknown, len: 163 aa 4 4 16 12 [Reporter]SCO2282 (11H5)_PCR/7900 [Gene]SCO2282/7191 NC_003888 SCC75A.28c, hypothetical protein, len: 326 aa; similar to TR:Q984U3 (EMBL:AP003012) Rhizobium loti (Mesorhizobium loti) MLL7840 protein 323 aa; fasta scores: opt: 712 Z-score: 794.8 E(): 1.2e-36; 38.365% identity in 318 aa overlap 1 2 3 18 [Reporter]SCO2029 (4I23)_PCR/2077 [Gene]SCO2029/1892 NC_003888 SC3A3.07, putative secreted protein, len: 425 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Shows weak similarity to the neighbouring CDS SC3A3.06, SC3A3.08 and SC3A3.09 e.g. SC3A3.06 possible membrane protein (509 aa) (32.1% identity in 383 aa overlap). Contains possible N-terminal region signal peptide sequence 4 4 16 11 [Reporter]SCO4795 (12D5)_PCR/7899 [Gene]SCO4795/7190 NC_003888 SCD63A.06c, possible integral membrane protein, len: 338 aa; similar to TR:O88074 (EMBL:AL031541) Streptomyces coelicolor putative integral membrane protein SCI35.39,394 aa; fasta scores: opt: 142 z-score: 173.6 E(): 0.033; 28.0% identity in 311 aa overlap. Contains possible hydrophobic membrane spanning regions 1 2 3 17 [Reporter]SCO6552 (5E23)_PCR/2076 [Gene]SCO6552/1891 NC_003888 SC4B5.02c, probable long-chain-fatty-acid-CoA ligase, len: 608aa, similar to many eg. TR:O53521 (EMBL:AL021957) putative long-chain-fatty-acid-CoA ligase from Mycobacterium tuberculosis (600 aa), fasta scores; opt: 1177, z-score: 1838.1, E(): 0, (44.8% identity in 612 aa overlap). Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme, score -73.00, E-value 7.8e-11 and PS00455 Putative AMP-binding domain signature. 1 2 3 16 [Reporter]SCO6721 (6A23)_PCR/2075 [Gene]SCO6721/1890 NC_003888 SC5F2A.04, possible integral membrane protein, len: 302aa; contains possible membrane spanning hydrophobic regions. 4 4 16 10 [Reporter]SCO3029 (13P1)_PCR/7898 [Gene]SCO3029/7189 NC_003888 SCE34.10c, possible L-lactate permease, len: 538 aa; similar to SW:LLDP_ECOLI (EMBL:L13970) Escherichia coli L-lactate permease LldP, 551 aa; fasta scores: opt: 825 z-score: 909.1 E(): 0; 40.0% identity in 552 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 16 9 [Reporter]SCO3762 (14L1)_PCR/7897 [Gene]SCO3762/7188 NC_003888 SCH63.09c, unknown, len: 65 aa 1 2 3 15 [Reporter]SCO4414 (7M19)_PCR/2074 [Gene]SCO4414/1889 NC_003888 SC6F11.12, possible integral membrane protein, len: 441 aa; similar to TR:BAB06413 (EMBL:AP001516) Bacillus halodurans transporter BH2694, 418 aa; fasta scores: opt: 795 z-score: 909.8 E(): 0; 31.0% identity in 413 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 16 8 [Reporter]SCO1626 (15H1)_PCR/7896 [Gene]SCO1626/7187 NC_003888 SCI41.09c, possible cytochrome P450, len: 498 aa. Similar to cytochrome P450 family, e.g. SW:CPXF_STRGO (EMBL:M32239) Streptomyces griseolus cytochrome P450-SU2 (EC 1.14.-.-) (P450-CVB, 402 aa); fasta scores: opt: 492 z-score: 485.2 E(): 1.2e-19; 31.0% identity in 407 aa overlap. Contains Pfam match to entry PF00067 p450, cytochrome P450. 1 2 3 13 [Reporter]SCO7155 (9E19)_PCR/2072 [Gene]SCO7155/1888 NC_003888 SC9A4.17, unknown, len: 355 aa 4 4 16 7 [Reporter]SCO1557 (16D1)_PCR/7895 [Gene]SCO1557/7186 NC_003888 SCL11.13c, possible lipoprotein, len: 275 aa; similar to TR:AAD51879 (EMBL:AF102556) Salmonella enteritidis liporpotein SfbA, 276 aa; fasta scores: opt: 519 z-score: 578.9 E(): 8e-25; 38.0% identity in 258 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 1 2 3 12 [Reporter]SCO2462 (10A19)_PCR/2071 [Gene]SCO2462/1887 NC_003888 SC7A8.01, probable sugar kinase (fragment), len: >434 aa; similar to TR:CAB61582 (EMBL:AL133210) Streptomyces coelicolor xylulose kinase (fragment) XylB, 432 aa; fasta scores: opt: 822 z-score: 871.9 E(): 0; 38.8% identity in 418 aa overlap and to SW:XYLB_STRRU (EMBL:M73789) Streptomyces rubiginosus xylulose kinase (EC 2.7.1.17) XylB, 481 aa; fasta scores: opt: 814 z-score: 862.8 E(): 0; 39.5% identity in 423 aa overlap . Contains Pfam match to entry PF00370 FGGY, FGGY family of carbohydrate kinases and PS00445 FGGY family of carbohydrate kinases signature 2,SCC24.33, probable sugar kinase (fragment), len: >82 aa; similar to SW:XYLB_STRRU (EMBL:M73789) Streptomyces rubiginosus xylulose kinase (EC 2.7.1.17) XylB, 481 aa; fasta scores: opt: 135 z-score: 170.4 E(): 0.05; 34.1% identity in 82 aa overlap 4 4 16 6 [Reporter]SCO0383 (16P21)_PCR/7894 [Gene]SCO0383/7185 NC_003888 SCF62.09, unknown, len: 407 aa. Weakly similar to a number of glycosyl transferases e.g. Streptomyces coelicolor TR:CAB39859 (EMBL; AL049497) putative glycosyl transferase SC6G10.05C (412 aa), fasta scores opt: 236 z-score: 280.6 E(): 2.8e-08 26.4% identity in 386 aa overlap and Escherichia coli TR:Q47594 (EMBL; D43637) mannosyltransferase B (381 aa), fasta scores opt: 187 z-score: 223.4 E(): 4.3e-05 23.3% identity in 288 aa overlap. Contains a TTA leucine codon, possible target for bldA regulation. 1 2 3 11 [Reporter]SCO4509 (11M15)_PCR/2070 [Gene]SCO4509/1886 NC_003888 SCD35.16, unknown, len: 124 aa. Contains possible coiled-coil region at aprox. residues 40..56 4 4 16 4 [Reporter]SCO0911 (18H21)_PCR/7892 [Gene]SCO0911/7184 NC_003888 SCM1,44, unknown, len: 446 aa. Similar to several proteins of undefined function e.g. from Mycobacterium tuberculosis TR:O69671(EMBL:AL022121) hypothetical 47.1 KD protein (425 aa), fasta scores opt: 1480 z-score: 1643.4 E():0 53.9% identity in 421 aa overlap. 1 2 3 10 [Reporter]SCO3293 (12I15)_PCR/2069 [Gene]SCO3293/1885 NC_003888 SCE15.10c, possible small integral membrane protein, len: 167aa; contains a possible membrane spanning hydrophobic region. 4 4 16 3 [Reporter]SCP1.189 (19D21)_PCR/7891 [Gene]SCP1.189/7183 NC_003888 SCP1.189, unknown, len: 379aa; 1 2 3 9 [Reporter]SCO1059 (13E15)_PCR/2068 [Gene]SCO1059/1884 NC_003888 SCG22.05, probable beta-glucosidase, len: 459 aa; similar to TR:Q59976 (EMBL:Z29625) Streptomyces sp. beta-glucosidase (EC 3.2.1.21) Bgl3, 479 aa; fasta scores: opt: 1627 z-score: 1895.8 E(): 0; 57.6% identity in 458 aa overlap. Contains Pfam match to entry PF00232 Glyco_hydro_1, Glycosyl hydrolase family 1 and match to Prosite entry PS00653 Glycosyl hydrolases family 1 N-terminal signature 4 4 16 2 [Reporter]SCO3983 (20L17)_PCR/7890 [Gene]SCO3983/7182 NC_003888 SCBAC25E3.20, unknown, len: 103 aa: no significant database matches. Contains TTA encoded leucine - possible target for bldA regulation. 1 2 3 8 [Reporter]SCO1077 (14A15)_PCR/2067 [Gene]SCO1077/1883 NC_003888 SCG22.23c, probable sugar kinase, len: 317 aa; similar to SW:GLK_BACSU (EMBL:D84432) Bacillus subtilis glucokinase (EC 2.7.1.2) GlcK, 321 aa; fasta scores: opt: 495 z-score: 504.0 E(): 1.3e-20; 31.4% identity in 312 aa overlap. Contains match to Prosite entry PS01125 ROK family signature 4 4 15 22 [Reporter]SCO1107 (1L13)_PCR/7888 [Gene]SCO1107/7181 NC_003888 2SCG4.23c, unknown, len: 151 aa 1 2 3 7 [Reporter]SCO3561 (15M11)_PCR/2066 [Gene]SCO3561/1882 NC_003888 SCH5.24c, possible lipoprotein, len: 431aa; Contains possible N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 4 4 15 21 [Reporter]SCO5042 (2H13)_PCR/7887 [Gene]SCO5042/7180 NC_003888 SCK7.15c, fumC, fumarate hydratase C, len: 461 aa; similar to SW:FUMC_PSEAE (EMBL:U59458) Pseudomonas aeruginosa fumarate hydratase C (EC 4.2.1.2) FumC, 458 aa; fasta scores: opt: 1505 z-score: 1638.8 E(): 0; 53.6% identity in 459 aa overlap. Contains Pfam match to entry PF00206 lyase_1, Lyase and match to Prosite entry PS00163 Fumarate lyases signature 1 2 3 6 [Reporter]SCO0504 (16I11)_PCR/2065 [Gene]SCO0504/1881 NC_003888 SCF34.23, probable DEAD-box RNA-helicase, len: 439 aa; similar to many e.g. to TR:O64430 (EMBL:AB010259), Drh1, Arabidopsis thaliana DEAD box ATPase/RNA helicase protein (619 aa), except where the A.thaliana protein is longer at the N-terminus, fasta scores; opt: 806 z-score: 892.9 E(): 0, 35.3% identity in 447 aa overlap. Also similar to many putative RNA-helicases e.g. SW:RHLE_ECOLI (EMBL:L02123), RhlE, Escherichia coli putative ATP-dependent RNA helicase (454 aa) (36.4% identity in 451 aa overlap) and to TR:O54116 (EMBL:AL021529) Streptomyces coelicolor probable DEAD-box RNA-helicase (498 aa) (67.7% identity in 424 aa overlap). Contains Pfam matches to entry PF00270 DEAD, DEAD/DEAH box helicase and to entry PF00271 helicase_C, Helicases conserved C-terminal domain. Contains 2 PS00017 ATP/GTP-binding site motif A (P-loop) 1 2 3 5 [Reporter]SCO0524 (17E11)_PCR/2064 [Gene]SCO0524/1880 NC_003888 SCF11.04, possible gluconolactonase, len: 302 aa; similar to SW:GNL_ZYMMO (EMBL:X67189) Zymomonas mobilis gluconolactonase precursor (EC 3.1.1.17) D-glucono-delta-lactone lactonohydrolase; fasta scores: opt: 326 z-score: 392.6 E(): 1.7e-14; 28.7% identity in 258 aa overlap 4 4 15 20 [Reporter]SCO2211 (3D13)_PCR/7886 [Gene]SCO2211/7179 NC_003888 SC10B7.06, unknown, len: 309 aa 4 4 15 19 [Reporter]SCO6943 (4P9)_PCR/7885 [Gene]SCO6943/7178 NC_003888 SC1G8.15c, cvnA8, possible sensor histidine-kinase, len: 505 aa. Highly similar to many proteins from Streptomyces coelicolor including: TR:CAB71241 (EMBL:AL138598) putative membrane protein, SC1A2.03 (650 aa), fasta scores opt: 700 z-score: 669.8 E(): 7.6e-30 34.2% identity in 524 aa overlap and TR:CAB76341 (EMBL:AL158061) putative sensor histidine-kinase, SC6D11.18C (543 aa), fasta scores opt: 433 z-score: 417.8 E(): 8.3e-16 28.6% identity in 482 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop) and a Pfam match to entry PF00512 signal, Histidine kinase. Also contains possible membrane spanning hydrophobic domains. 1 2 3 4 [Reporter]SCO0128 (18A11)_PCR/2063 [Gene]SCO0128/1879 NC_003888 SCJ21.09, unknown, len: 60 aa 4 4 15 18 [Reporter]SCO2084 (5L9)_PCR/7884 [Gene]SCO2084/7177 NC_003888 SC4A10.17c, murG, probable UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase, len: 364 aa; previously sequenced as TR:Q9ZBA5 (EMBL:U10879), MurG, Streptomyces coelicolor UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (364 aa) and identical to that sequence. Similar to many e.g. TR:O07109 (EMBL:U94707), MurG, Enterococcus faecalis UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (363 aa), fasta scores; opt: 648 z-score: 711.4 E(): 2.8e-32, 33.2% identity in 365 aa overlap 1 2 3 3 [Reporter]SCP1.169 (19M7)_PCR/2062 [Gene]SCP1.169/1878 NC_003888 SCP1.169, possible ATP/GTP-binding protein, len: 537aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 4 15 17 [Reporter]SCO5783 (6H9)_PCR/7883 [Gene]SCO5783/7176 NC_003888 SC4H2.04c, hypothetical protein, len: 161 aa; similar to hypothetical proteins from several organisms e.g. M. tuberculosis TR:O05882 (EMBL:Z95121) MTCY20B11.12C (160 aa), fasta scores; opt: 315 z-score: 557.4 E(): 8e-24, 40.0% identity in 150 aa overlap (also upstream of a putative transport protein) 1 2 3 2 [Reporter]SCP1.305 (20E7)_PCR/2061 [Gene]SCP1.305/1877 NC_003888 SCP1.305, unknown, len: 296aa; 4 4 15 15 [Reporter]SCO7249 (8P5)_PCR/7881 [Gene]SCO7249/7175 NC_003888 SC7A12.16, unknown, len: 165 aa. 1 2 2 22 [Reporter]SCO5197 (1E3)_PCR/2059 [Gene]SCO5197/1876 NC_003888 2SC3B6.21, conserved hypothetical protein, len: 251 aa; similar to TR:CAB93731 (EMBL:AL357613) Streptomyces coelicolor hypothetical 23.8 kD protein SC5F8.03c, 225 aa; fasta scores: opt: 449 z-score: 539.1 E(): 1.5e-22; 35.7% identity in 230 aa overlap 4 4 15 14 [Reporter]SCO7825 (9L5)_PCR/7880 [Gene]SCO7825/7174 NC_003888 SC8E7.22, doubtful CDS, len: 98 aa. 1 2 2 21 [Reporter]SCO0736 (2A3)_PCR/2058 [Gene]SCO0736/1875 NC_003888 3SC5B7.14c, possible secreted protein, len: 231 aa. Contains possible N-terminal region cleavable signal peptide sequence 4 4 15 13 [Reporter]SCO5714 (10H5)_PCR/7879 [Gene]SCO5714/7173 NC_003888 SC9F2.02, possible peptide transport permease, len: 317aa; similar to many (confirmed and proposed) eg. SW:APPC_BACSU oligopeptide transport permease, AppC, from Bacillus subtilis (303 aa) fasta scores; opt: 638, z-score: 718.2, E(): 1.1e-32, (36.5% identity in 277 aa overlap). Contains probable membrane spanning hydrophobic regions. Also contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 42.30, E-value 1.1e-08. 1 2 2 20 [Reporter]SCO4563 (2M23)_PCR/2057 [Gene]SCO4563/1874 NC_003888 SCD16A.20c, nuoB, NADH dehydrogenase subunit, len: 184 aa; similar to many e.g. TR:P95180 (EMBL:Z83867) NuoB, hypothetical protein from Mycobacterium tuberculosis (184 aa) fasta scores; opt: 995, z-score: 1189.3, E(): 0, (78.8% identity in 184 aa overlap) and SW:NUOB_ECOLI, NADH dehydrogenase subunit NuoB from Escherichia coli (220 aa) fasta scores; opt: 607, z-score: 729.2, E(): 0, (53.4% identity in 148 aa overlap). Contains Pfam match to entry PF01058 oxidored_q6, NADH ubiquinone oxidoreductase, 20 Kd subunit, score 270.90, E-value 1.7e-77. 4 4 15 12 [Reporter]SCO2278 (11D5)_PCR/7878 [Gene]SCO2278/7172 NC_003888 SCC75A.24, unknown, len: 295 aa. 1 2 2 19 [Reporter]SCO6138 (3I23)_PCR/2056 [Gene]SCO6138/1873 NC_003888 SC1A9.02, possible transmembrane protein, len: 307aa; Contains several possible membrane spanning domains. 4 4 15 11 [Reporter]SCO4494 (12P1)_PCR/7877 [Gene]SCO4494/7171 NC_003888 SCD35.01, unknown, len: >95 aa,SCD69.14, conserved hypothetical protein (fragment), len: >358 aa; similar to TR:AAF09948 (EMBL:AE001897) Deinococcus radiodurans conserved hypothetical protein DR0366, 376 aa; fasta scores: opt: 896 z-score: 1100.7 E(): 0; 39.8% identity in 357 aa overlap 1 2 2 18 [Reporter]SCO6195 (4E23)_PCR/2055 [Gene]SCO6195/1872 NC_003888 SC2G5.16, probable acetyl-coenzyme A synthetase, len:558 aa; similar to many e.g. SW:ACSA_BACSU from Bacillus subtilis (572 aa) fasta scores; opt: 798, z-score: 878.3,E():0, (31.8% identity in 537 aa overlap). Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme, score 116.90, E-value 3.8e-31. Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme, score 116.90, E-value 3.8e-31 4 4 15 10 [Reporter]SCO3021 (13L1)_PCR/7876 [Gene]SCO3021/7170 NC_003888 SCE34.02, possible integral membrane protein, len: 318 aa; similar to TR:O69663 (EMBL:AL022121) Mycobacterium tuberculosis hypothetical 33.2 kD protein MTV025.043, 310 aa; fasta scores: opt: 734 z-score: 602.4 E(): 4.3e-26; 42.9% identity in 319 aa overlap. Contains possible hydrophobic membrane spanning regions 1 2 2 17 [Reporter]SCO7134 (5A23)_PCR/2054 [Gene]SCO7134/1871 NC_003888 SC4B10.35, probable transcriptional regulatory protein, len: 923 aa; similar to TR:Q9S1Z4 (EMBL:AL109747) Streptomyces coelicolor probable transcriptional regulator SCJ21.13, 919 aa; fasta scores: opt: 2675 z-score: 2853.2 E(): 0; 50.6% identity in 926 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Also contains possible helix-turn-helix motif at residues 876..897 (+3.18 SD) 1 2 2 16 [Reporter]SCO7299 (6M19)_PCR/2053 [Gene]SCO7299/1870 NC_003888 SC5F8.09, possible stress-inducible protein, len:312 aa. Highly similar to many proteins of undefined function e.g. Streptomyces coelicolor TR:Q9RIY4(EMBL:AL109962) hypothetical 34.7 kd protein, SCJ1.30C (328 aa), fasta scores opt: 604 z-score: 654.8 E(): 5.3e-29 41.8% identity in 328 aa overlap. Contains 2xPfam match to entry PF00582 Usp, Universal stress protein family. 4 4 15 9 [Reporter]SCO0238 (14H1)_PCR/7875 [Gene]SCO0238/7169 NC_003888 SCJ9A.17, hypothetical protein, len: 175 aa; similar to TR:Q9RI91 (EMBL:AL109949) Streptomyces coelicolor hypothetical protein SCJ11.13, 152 aa; fasta scores: opt: 683 Z-score: 798.0 E(): 8.1e-37; 68.421% identity in 152 aa overlap 4 4 15 8 [Reporter]SCO1733 (15D1)_PCR/7874 [Gene]SCO1733/7168 NC_003888 SCI11.22c, possible acetyltransferase, len: 156 aa; unknown function, weakly similar to TR:Q51670 (EMBL:U34346) Paracoccus denitrificans hypothetical protein (173 aa), fasta scores; opt: 144 z-score: 184.8 E(): 0.0058, 28.9% identity in 149 aa overlap. Very weak similarity to some acetyltransferases e.g. SW:IAAT_AZOBR (EMBL:X57853) Azospirillum brasilense IAA acetyltransferase (153 aa) (26.1% identity in 111 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 2 2 15 [Reporter]SCO4410 (7I19)_PCR/2052 [Gene]SCO4410/1869 NC_003888 SC6F11.08, possible anti anti sigma factor, len: 126 aa; similar to SW:RSBV_STRCO (EMBL:AF134889) Streptomyces coelicolor anti-sigma B factor antagonist BldG, 113 aa; fasta scores: opt: 195 z-score: 264.4 E(): 3.2e-07; 30.8% identity in 104 aa overlap. Contains Pfam match to entry PF01740 STAS, STAS domain 4 4 15 7 [Reporter]SCO3637 (15P21)_PCR/7873 [Gene]SCO3637/7167 NC_003888 SCH10.15, putative membrane protein, len: 145 aa; unknown function, probable CDS suggested by positional base preference and GC frame analysis. N-terminal half is hydrophobic, while remainder is hydrophilic. Contains possible hydrophobic membrane spanning regions 1 2 2 14 [Reporter]SCO6873 (8E19)_PCR/2051 [Gene]SCO6873/1868 NC_003888 SC7F9.25c, unknown, len: 80 aa. 4 4 15 6 [Reporter]SCO0236 (16L21)_PCR/7872 [Gene]SCO0236/7166 NC_003888 SCJ9A.15c, possible DNA-binding protein, len: 290 aa; prediction based on GC Frameplot, Hidden Markov Model and amino acid usage. Similar to TR:CAC44562 (EMBL:AL596144) Streptomyces coelicolor putative DNA-binding protein SCBAC12C8.07c, 284 aa; fasta scores: opt: 636 Z-score: 730.2 E(): 4.9e-33; 43.060% identity in 281 aa overlap. Contains possible helix-turn-helix motif (+3.31 SD) residues 39-60 1 2 2 13 [Reporter]SCO7149 (9A19)_PCR/2050 [Gene]SCO7149/1867 NC_003888 SC9A4.11, possible lyase, len: 151 aa; similar to TR:Q9XA78 (EMBL:AL096837) Streptomyces coelicolor putative lyase SCF43A.37c, 152 aa; fasta scores: opt: 679 z-score: 811.2 E(): 0; 69.7% identity in 145 aa overlap and to SW:BHC3_RHOGO (EMBL:X75635) Rhodococcus globerulus biphenyl-2,3-diol 1,2-dioxygenase III (EC 1.13.11.39) BphC3, 189 aa; fasta scores: opt: 146 z-score: 184.1 E(): 0.0087; 29.3% identity in 123 aa overlap 4 4 15 5 [Reporter]SCO0378 (17H21)_PCR/7871 [Gene]SCO0378/7165 NC_003888 SCF62.04c, possible secreted protein, len: 244 aa. Highly similar to Streptomyces coelicolor TR:CAB52958 (EMBL; AL109950) hypothetical 25.2 KD protein SCJ4.24C (242 aa), fasta scores opt: 1283 z-score: 1457.6 E(): 0 83.1% identity in 242 aa overlap. Contains a possible N-terminal signal sequence. 1 2 2 12 [Reporter]SCO2338 (10M15)_PCR/2049 [Gene]SCO2338/1866 NC_003888 SCC53.29, possible O-methyltransferase, len: 223 aa; similar to many e.g. SW:Q00719 (MDMC_STRMY) macrolide O-methyltransferase (EC 2.1.1.-) from Streptomyces mycarofaciens (221 aa) fasta scores; opt: 405, z-score: 484.6, E(): 1.6e-19, 41.1% identity in 190 aa overlap. Contains Pfam match to entry PF01596 Methyltransf_3, O-methyltransferase. 4 4 15 4 [Reporter]SCO0027 (18D21)_PCR/7870 [Gene]SCO0027/7164 NC_003888 SCJ4.08c, doubtful CDS, function unknown, len: 122 aa 1 2 2 11 [Reporter]SCO4504 (11I15)_PCR/2048 [Gene]SCO4504/1865 NC_003888 SCD35.11c, possible methyltransferase, len: 241 aa; similar to TR:CAB61165 (EMBL:AL132973) Streptomyces coelicolor putative methyltransferase SCF91.08, 231 aa; fasta scores: opt: 214 z-score: 264.5 E(): 2.7e-07; 29.1% identity in 206 aa overlap and to SW:BIOC_SERMA (EMBL:D17468) Serratia marcescens biotin synthesis protein BioC, 255 aa; fasta scores: opt: 179 z-score: 222.0 E(): 6.2e-05; 34.0% identity in 100 aa overlap 4 4 15 3 [Reporter]SCP1.31 (19P17)_PCR/7869 [Gene]SCP1.31/7163 NC_003888 SCP1.31, possible secreted protein, len: 219aa; similar to TR:Q9KY42 (EMBL:AL356892) hypothetical protein from Streptomyces coelicolor (214 aa) fasta scores; opt: 499, z-score: 600.0, E(): 5.9e-26, 41.9% identity in 210 aa overlap. Also strongly similar over full length to CDS SCP1.267 (fasta scores; opt: 1190, z-score: 1349.2, E(): 0, 77.5% identity in 218 aa overlap) and C-terminus strongly similar to its neighbouring CDS SCP1.268 (111 aa) (fasta scores; opt: 401, z-score: 463.8, E(): 2.6e-21, 57.4% identity in 108 aa overlap). Contains a possible cleavable N-terminal signal sequence. 1 2 2 10 [Reporter]SCO4252 (12E15)_PCR/2047 [Gene]SCO4252/1864 NC_003888 SCD8A.25c, unknown, len: 149 aa 4 4 15 2 [Reporter]SCO5971 (20H17)_PCR/7868 [Gene]SCO5971/7162 NC_003888 SCBAC16H6.06, conserved hypothetical protein, len: 307 aa: similar to many e.g. TR:AAK45058 (EMBL:AE006971) hypothetical protein from Mycobacterium tuberculosis (347 aa) fasta scores; opt: 482, Z-score: 543.4, 32.399% identity (37.011% ungapped) in 321 aa overlap. Contains Pfam match to entry PF00296 bac_luciferase, Bacterial luciferase 1 2 2 9 [Reporter]SCO0049 (13A15)_PCR/2046 [Gene]SCO0049/1863 NC_003888 SCJ4.30, unknown, len: 107 aa 4 4 14 22 [Reporter]SCO1285 (1H13)_PCR/7866 [Gene]SCO1285/7161 NC_003888 2SCG18.32c, possible ketoreductase, len: 373 aa; similar to N-terminal region of TR:O50417 (EMBL:AL009198) Mycobacterium tuberculosis multi-functional enzyme MTV004.49, 650 aa; fasta scores: opt: 215 z-score: 237.4 E(): 9.7e-06; 30.5% identity in 377 aa overlap and to TR:Q9XC69 (EMBL:AF147704) Streptomyces fradiae MDP-hexose 4-ketoreductase TylCIV, 347 aa; fasta scores: opt: 182 z-score: 205.6 E(): 0.00057; 26.8% identity in 291 aa overlap and to Streptomyces coelicolor 2SCG18.20, 372 aa; fasta scores: opt: 241 z-score: 231.1 E(): 2.8e-07; 27.0% identity in 330 aa overlap 1 2 2 8 [Reporter]SCO3589 (14M11)_PCR/2045 [Gene]SCO3589/1862 NC_003888 SCH66.10c, probable two component sensor kinase, len: 364 aa; similar to many e.g. SW:CUTS_STRCO two component sensor kinase involved in the regulation of copper uptake in Streptomyces coelicolor (414 aa) fasta scores; opt: 659, z-score: 768.1, E(): 0, (35.6% identity in 390 aa overlap). Contains Pfam matches to entry PF00512 signal, Signal carboxyl-terminal domain and to entry PF00672 DUF5, Domain found in bacterial signal proteins. Contains possible hydrophobic membrane spanning regions 4 4 14 21 [Reporter]SCO0998 (2D13)_PCR/7865 [Gene]SCO0998/7160 NC_003888 2SCG2.11, ftrE, Fe uptake system permease, len: 272 aa; similar to previously sequenced and annotated as TR:Q9X470 (EMBL:AF099015) Streptomyces coelicolor Fe uptake system permease FtrE, 271 aa; fasta scores:opt: 1424 z-score: 1545.2 E(): 0; 89.5% identity in 257 aa overlap and similar to TR:CAB94640 (EMBL:AL359215) Streptomyces coelicolor putative iron transport protein, ATP-binding component SC2H12.16, 258 aa; fasta scores: opt: 834 z-score: 672.9 E(): 7.8e-32; 55.4% identity in 251 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 2 2 7 [Reporter]SCO0050 (15I11)_PCR/2044 [Gene]SCO0050/1861 NC_003888 SCJ4.31, hypothetical protein, len: 111 aa; region of similarity to many respiratory electron transport chain proteins eg. TR:O49078 (EMBL:AF037456) cytochrome B6-F complex iron-sulphur subunit from Fritillaria agrestis (230 aa) fasta scores; opt: 104, z-score: 143.7, E(): 1.2, (32.8% identity in 67 aa overlap). 1 2 2 6 [Reporter]SCO1890 (16E11)_PCR/2043 [Gene]SCO1890/1860 NC_003888 SCI7.08c, probable transcriptional regulator, len: 177aa; similar to many egs. TR:O50514 (EMBL:AL009204) putative transcriptional regulator from Streptomyces coelicolor (231 aa) fasta scores; opt: 272, z-score: 328.8, E(): 5.6e-11, 934.4% identity in 224 aa overlap) and SW:GNTR_BACSU transcriptional regulator of the gluconate operon of Bacillus subtilis (243 aa) fasta scores; opt: 217, z-score: 264.1, E(): 2.2e-07, (33.1% identity in 127 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. Also contains a helix-turn-helix motif (+3.07 SD) 35-56aa. 4 4 14 20 [Reporter]SCO5901 (3P9)_PCR/7864 [Gene]SCO5901/7159 NC_003888 SC10A5.06, unknown, len: 458; similar to M. tuberculosis hypothetical protein TR:O07191 (EMBL:Z96072) MTCY05A6. 10C (405 aa), fasta scores; opt: 455 z-score: 878.5 E(): 0, 38.4% identity in 417 aa overlap, and weakly similar to several putative RNA methyltransferases eg. YGCA_ECOLI P5513 5 hypothetical RNA methyltransferase (433 aa), fasta scores ; opt: 358 z-score: 331.2 E(): 3e-11, 27.4% identity in 441 aa overlap 4 4 14 19 [Reporter]SCO6939 (4L9)_PCR/7863 [Gene]SCO6939/7158 NC_003888 SC1G8.11c, unknown, len: 213 aa. Similar to Streptomyces coelicolor TR:CAB71245(EMBL:AL138598) hypothetical 20.6 kd protein, SC1A2.07 (191 aa), fasta scores opt: 376 z-score: 435.5 E(): 8.6e-17 43.9% identity in 164 aa overlap. 1 2 2 5 [Reporter]SCO4731 (17A11)_PCR/2042 [Gene]SCO4731/1859 NC_003888 SC6G4.09, truA, probable pseudouridylate synthase, len: 284 aa; similar to many e.g. TRUA_ECOLI pseudouridylate synthase I (EC 4.2.1.70) (270 aa), fasta scores; opt: 466 z-score: 630.6 E(): 7.4e-28, 35.1% identity in 265 aa over lap 4 4 14 18 [Reporter]SCO6406 (5H9)_PCR/7862 [Gene]SCO6406/7157 NC_003888 SC3C8.25c, possible secreted protein, len: 131 aa; contains possible N-terminal signal sequence 1 2 2 4 [Reporter]SCO3732 (18M7)_PCR/2041 [Gene]SCO3732/1858 NC_003888 SCH22A.10, probable DEAD-box RNA helicase, len: 528 aa; similar to TR:O54116 (EMBL:AL021529) Streptomyces coelicolor DEAD-box RNA helicase SC10A5.25c, 498 aa; fasta scores: opt: 2348 z-score: 2252.6 E(): 0; 74.9% identity in 506 aa overlap and to SW:RHLE_ECOLI (EMBL:L02123) Escherichia coli putative ATP-dependent RNA helicase RhlE, 454 aa; fasta scores: opt: 874 z-score: 843.8 E(): 0; 36.6% identity in 470 aa overlap. Contains two Pfam matches to entries PF00270 DEAD, DEAD/DEAH box helicase and PF00271 helicase_C, Helicases conserved C-terminal domain, and two matches to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 4 14 17 [Reporter]SCO2068 (6D9)_PCR/7861 [Gene]SCO2068/7156 NC_003888 SC4A10.01, hypothetical protein, partial CDS, len: >67 aa; unknown function, probable CDS suggested by GC frameplot and positional base preference. Continues in cosmid 4G6 as SC4G6.39,SC4G6.37, possible secreted alkaline phosphatase, len: >517aa; similar to SW:PPBD_BACSU alkaline phosphatase D from Bacillus subtilis (556 aa) fasta scores; opt: 837, z-score: 915.3, E(): 0, (34.3% identity in 539 aa overlap). Contains possible N-terminal region signal sequence peptide 1 2 2 3 [Reporter]SCP1.246 (19I7)_PCR/2040 [Gene]SCP1.246/1857 NC_003888 SCP1.246, mmyR, possible lactone-dependent transcriptional regulator (TetR-family), len: 203aa; previously sequenced and annotated as hypothetical protein TR:Q9JN93 (EMBL:AJ276673). Weakly similar to many lactone-dependent transcriptional regulators eg. CprS protein from Streptomyces coelicolor (215 aa) fasta scores; opt: 240, z-score: 299.2, E(): 3.3e-09, 26.9% identity in 193 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and helix-turn-helix motif at residue 29-50 (Score 1129 (+3.03 SD)). 4 4 14 16 [Reporter]SCO5436 (7P5)_PCR/7860 [Gene]SCO5436/7155 NC_003888 SC6A11.12, possible sodium:dicarboxylate symporter, len: 466 aa; similar to many e.g. SW:P50334 (DCTA_SALTY) C4-dicarboxylate transport protein from Salmonella typhimurium (428 aa) fasta scores: opt: 1320, z-score: 1451.6, E(): 0, 48.9% identity in 419 aa overlap. Contains Pfam match to entry PF00375 SDF, Sodium:dicarboxylate symporter family 1 2 2 2 [Reporter]SCO2236 (20A7)_PCR/2039 [Gene]SCO2236/1856 NC_003888 SCBAC17D6.03, conserved hypothetical protein, len: 84 aa; identical to previously sequenced TR:Q9Z4V8 (EMBL:Y17736) Streptomyces coelicolor hypothetical 10.0 kDa protein, 84 aa and similar to SW:YOEB_ECOLI (EMBL:AE000293) Escherichia coli hypothetical 10.2 kDa protein in sbcB-hisL intergenic region, 84 aa; fasta scores: opt: 354 Z-score: 476.5 bits: 92.5 E(): 5.9e-19; 53.571% identity in 84 aa overlap 4 4 14 14 [Reporter]SCO7727 (9H5)_PCR/7858 [Gene]SCO7727/7154 NC_003888 SC8D11.18c, possible marR-family regulatory protein, len: 155 aa; similar to TR:Q9X5U1 (EMBL:AF127374) Streptomyces lavendulae repressor MmcW, 163 aa; fasta scores: opt: 351 z-score: 450.9 E(): 1.4e-17; 36.2% identity in 152 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 1 2 1 21 [Reporter]SCO4929 (1M23)_PCR/2036 [Gene]SCO4929/1855 NC_003888 SCK13.21c, possible membrane protein, len: 221 aa. High content in proline amino acid residues and in G+C (81.74%). Contains possible hydrophobic membrane spanning region 4 4 14 13 [Reporter]SCO5133 (10D5)_PCR/7857 [Gene]SCO5133/7153 NC_003888 SC9E12.18c, hypothetical protein, len: 298 aa; C-terminal highly similar to N-terminal domain of TR:Q07641 (EMBL:AB006206) Streptomyces griseus hypothetical 34.5 kDa protein in AmfR 3'region (fragment), 87 aa; fasta scores: opt: 371 z-score: 414.3 E(): 1.3e-15; 63.2% identity in 87 aa overlap 1 2 1 20 [Reporter]SCO5225 (2I23)_PCR/2035 [Gene]SCO5225/1854 NC_003888 SC7E4.22c, nrdM, ribonucleotide-diphosphate reductase small chain, len: 343 aa; identical to previously sequenced TR:CAB82486 (EMBL:AJ276618) Streptomyces coelicolor A3(2) ribonucleotide-diphosphate reductase small chain (EC 1.17.4.1) NrdM, 343 aa. Contains Pfam match to entry PF00268 ribonuc_red, Ribonucleotide reductases and a possible hydrophobic membrane spanning region 4 4 14 12 [Reporter]SCO2352 (11P1)_PCR/7856 [Gene]SCO2352/7152 NC_003888 SCC8A.10c, unknown, len: 77aa; 1 2 1 19 [Reporter]SCO7401 (3E23)_PCR/2034 [Gene]SCO7401/1853 NC_003888 SC10G8.29c, unknown, len: 189 aa. Similar to Deinococcus radiodurans TR:AAF11944 (EMBL:AE002070) Conserved hypothetical protein (200 aa), fasta scores opt: 394 z-score: 504.8 E(): 1.1e-20 42.9% identity in 156 aa overlap 4 4 14 11 [Reporter]SCO4490 (12L1)_PCR/7855 [Gene]SCO4490/7151 NC_003888 SCD69.10, putative decarboxylase, len: 485 aa; similar to SW:Y209_ARCFU (EMBL:AE001091) Archaeoglobus fulgidus hypothetical protein AF0209, 481 aa; fasta scores: opt: 1557 z-score: 1800.8 E(): 0; 48.6% identity in 484 aa overlap and to SW:P26615 (UBID_ECOLI) 3-Octaprenyl-4-hydroxybenzoate carboxy-lyase from Escherichia coli (497 aa) fasta scores; opt: 1293, Z-score: 1478.5, 40.162% identity (41.336% ungapped) in 493 aa overlap. Contains Pfam match to entry PF01977 UPF0096, Protein of unknown function. 1 2 1 18 [Reporter]SCO6191 (4A23)_PCR/2033 [Gene]SCO6191/1852 NC_003888 SC2G5.12c, unknown, len: 304aa 4 4 14 10 [Reporter]SCO2857 (13H1)_PCR/7854 [Gene]SCO2857/7150 NC_003888 SCE20.31c, possible membrane protein, len: 114 aa. Contains possible membrane spanning hydrophobic domains and an N-terminal signal sequence. 1 2 1 17 [Reporter]SCO6715 (5M19)_PCR/2032 [Gene]SCO6715/1851 NC_003888 SC4C6.25, probable transcriptional regulator, len: 81 aa, similar to many transcriptional regulators (some putative) from Streptomyces e.g. TR:Q53963 (EMBL:X62287), WhiB, S.coelicolor transcriptional regulator essential for sporulation (87 aa), fasta scores; opt: 336 z-score: 452.7 E(): 5.7e-18, 61.1% identity in 72 aa overlap. Also similar to e.g. TR:O69649 (EMBL:AL02212) Mycobacterium tuberculosis putative regulatory protein (100 aa) (40.0% identity in 75 aa overlap) 1 2 1 16 [Reporter]SCO7295 (6I19)_PCR/2031 [Gene]SCO7295/1850 NC_003888 SC5F8.05c, possible LuxR-family regulator, len: 988 aa. Similar to several other putative regulators from Streptomyces coelicolor including: TR:Q9XAN4 (EMBL:AL079355) SC4C6.06 (893 aa), fasta scores opt: 305 z-score: 328.7 E(): 7.7e-11 26.4% identity in 910 aa overlap and TR:Q9Z573 (EMBL:AL035569) SC8D9.18 (1091 aa), fasta scores opt: 857 z-score: 926.1 E(): 0 36.5% identity in 1077 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop) Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family. 4 4 14 9 [Reporter]SCO0549 (14D1)_PCR/7853 [Gene]SCO0549/7149 NC_003888 SCF11.29c, possible acyl carrier protein, len: 92 aa; similar to SW:ACP_HAEIN (EMBL:U32701) Haemophilus influenza AcpP, 76 aa; fasta scores: opt: 162 z-score: 219.6 E(): 7.3e-05; 47.3% identity in 55 aa overlap. Contains Pfam match to entry PF00550 pp-binding, phosphopantetheine attachment site 4 4 14 8 [Reporter]SCO3479 (14P21)_PCR/7852 [Gene]SCO3479/7148 NC_003888 SCE65.15c, possible beta-galatosidase, len: 995 aa; similar to SW:BGAL_ECOLI (EMBL:J01636) Escherichia coli beta-galactosidase (EC 3.2.1.23) (lactase) lacZ, 1023 aa; fasta scores: opt: 1594 z-score: 1693.3 E(): 0; 33.6% identity in 1039 aa overlap. Contains Pfam match to entry PF00703 Glyco_hydro_2, Glycosyl hydrolases family and match to Prosite entry PS00719 Glycosyl hydrolases family 2 signature 1 1 2 1 15 [Reporter]SCO2680 (7E19)_PCR/2030 [Gene]SCO2680/1849 NC_003888 SCC61A.01, conserved hypothetical protein, partial CDS, len: >153 aa; similar to others of unknown function from Streptomyces coelicolor eg. TR:O86768 (EMBL:AL031035) putative ATP /GTP-binding protein (886 aa) fasta scores; opt: 457, z-score: 460.9, E(): 3.2e-18, 48.6% identity in 146 aa overlap. Searches suggest a frameshift mutation between CDSs 01 and 02.,SC6D10.23, hypothetical protein (partial), len: >85 aa; highly similar to N-terminal region of TR:O86768 (EMBL:AL031035) Streptomyces coelicolor putative ATP /GTP-binding= 333 z-score: 478.0 E(): 3.2e-19; 59.4% identity in 69 aa overlap 4 4 14 7 [Reporter]SCO0925 (15L21)_PCR/7851 [Gene]SCO0925/7147 NC_003888 SCM10.13, probable lysR-family transcriptional regulator, len: 294 aa; similar to SW:GLTC_BACSU (EMBL:M28509) Bacillus subtilis transcriptional regulator protein GltC, 300 aa; fasta scores: opt: 539 z-score: 638.5 E(): 3.6e-28; 33.6% identity in 283 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Contains a possible helix-turn-helix motif at residues 16..37 (+4.14 SD) 1 2 1 14 [Reporter]SCO6869 (8A19)_PCR/2029 [Gene]SCO6869/1848 NC_003888 SC7F9.21c, unknown, len: 247 aa. 4 4 14 6 [Reporter]SCO0490 (16H21)_PCR/7850 [Gene]SCO0490/7146 NC_003888 SCF34.09, possible esterase, len: 363 aa; similar to enterochelin esterases e.g. SW:FES_ECOLI (EMBL:J04216), Fes, Escherichia coli enterochelin esterase (374 aa), fasta scores; opt: 422 z-score: 474.2 E(): 4.7e-19, 32.5% identity in 332 aa overlap 1 2 1 13 [Reporter]SCO6116 (9M15)_PCR/2028 [Gene]SCO6116/1847 NC_003888 SC9B2.03, conserved hypothetical protein, len: 513 aa; similar to TR:CAC49227 (EMBL:AL603645) Rhizobium meliloti conserved hypothetical protein SMB21161, 477 aa; fasta scores: opt: 1078 Z-score: 1182.9 E(): 2.9e-58; 40.678% identity in 472 aa overlap 4 4 14 5 [Reporter]SCO3418 (17D21)_PCR/7849 [Gene]SCO3418/7145 NC_003888 SCE9.25c, probable ABC transporter ATP-binding component, len: 315 aa; similar to many e.g SW:DRRA_STRPE (EMBL:M73758), drrA, Streptomyces peucetius daunorubicin resistance ATP-binding protein (330 aa), fasta scores; opt: 942 z-score: 1020.6 E(): 0, 51.4% identity in 317 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, score 187.90, E-value 1.6e-52, PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 1 2 1 12 [Reporter]SCO2334 (10I15)_PCR/2027 [Gene]SCO2334/1846 NC_003888 SCC53.25, possible integral membrane protein, len: 551aa; contains possible membrane-spanning hydrophobic regions. Similar to TR:CAB88445 (EMBL:AL353815) putative integral membrane protein from Streptomyces coelicolor (625 aa) fasta scores; opt: 1266, z-score: 1408.1, E(): 0, 42.4% identity in 502 aa overlap and TR:Q9RZ17 (EMBL:AE001862) proposed amino acid abc transporter, permease protein from Deinococcus radiodurans (484 aa) fasta scores; opt: 138, z-score: 156.1, E(): 0.32, 29.6% identity in 321 aa overlap. 4 4 14 4 [Reporter]SCP1.273c (18P17)_PCR/7848 [Gene]SCP1.273c/7144 NC_003888 SCP1.273c, possible large secreted protein, len: 1039aa; similar to others eg. TR:Q9RKV7 (EMBL:AL132997) putative serine/threonine protein kinase SC9G1.09 from Streptomyces coelicolor fasta scores: E(): 0, 32.4% id in 675 aa, and to SW:WAPA_BACSU (Q07833) wall-associated protein precursor WapA from Bacillus subtilis fasta scores: E(): 0.00021, 21.9% id in 1078 aa. Contains a possible cleavable N-terminal signal sequence. 1 2 1 11 [Reporter]SCO4110 (11E15)_PCR/2026 [Gene]SCO4110/1845 NC_003888 SCD17A.02c, possible integral membrane protein, len: 463 aa. Contains possible hydrophobic membrane spanning regions 4 4 14 3 [Reporter]SCP1.205c (19L17)_PCR/7847 [Gene]SCP1.205c/7143 NC_003888 SCP1.205c, unknown, len: 841aa; weak but full length similarity to TR:O87242 (EMBL:AE001272) hypothetical protein from plasmid pMRC01 of Lactococcus lactis blastp scores; Identities = 223/824 (27%), Positives = 406/824 (49%). 1 2 1 10 [Reporter]SCO4248 (12A15)_PCR/2025 [Gene]SCO4248/1844 NC_003888 SCD8A.21c, unknown, len: 140 aa 4 4 14 2 [Reporter]SCO5982 (20D17)_PCR/7846 [Gene]SCO5982/7142 NC_003888 SCBAC16H6.17c, possible regulator, len: 281 aa: weakly similar to SW:P76086 (PAAX_ECOLI) phenylacetic acid degradation repressor protein from Escherichia coli (316 aa) fasta scores; opt: 266, Z-score: 305.3, 26.071% identity (28.077% ungapped) in 280 aa overlap. 1 2 1 9 [Reporter]SCO3447 (13M11)_PCR/2024 [Gene]SCO3447/1843 NC_003888 SCE46.04c, possible transcriptional regulatory protein, len: 227 aa; similar to TR:O53922 (EMBL:AL022002) Mycobacterium tuberculosis hypothetical 26.8kD protein MTV047.10c, 244 aa; fasta scores: opt: 486 z-score: 603.1 E(): 3e-26; 40.5% identity in 220 aa overlap. Contains Pfam matches to entries PF00325 crp, Bacterial regulatory proteins, crp family and PF00027 cNMP_binding, Cyclic nucleotide-binding domain. Contains a possible helix-turn-helix motif at residues 176..197 (+4.24 SD) 4 4 13 22 [Reporter]SCO1281 (1D13)_PCR/7844 [Gene]SCO1281/7141 NC_003888 2SCG18.28c, possible oxidoreductase, len: 440 aa; similar to SW:ORDL_ECOLI (EMBL:U38543) Escherichia coli probable oxidoreductase OrdL, 426 aa; fasta scores: opt: 273 z-score: 283.9 E(): 2.5e-08; 27.7% identity in 433 aa overlap 1 2 1 8 [Reporter]SCO3558 (14I11)_PCR/2023 [Gene]SCO3558/1842 NC_003888 SCH5.21, probable morphological differentiation-associated protein, len: 268 aa; almost identical to TR:O33611 (EMBL:AB004855) protein associated with inhibition of morphological differentiation in Streptomyces cyaneus (277 aa) fasta scores; opt: 1655, z-score: 1949.5, E(): 0, (93.7% identity in 268 aa overlap). Contains possible hydrophobic membrane spanning region at C-terminal domain 4 4 13 21 [Reporter]SCO4394 (2P9)_PCR/7843 [Gene]SCO4394/7140 NC_003888 SCD10.26, desR, probable iron repressor, len: 230 aa; identical to TR:Q54343 (EMBL:Z50049) Streptomyces lividans putative iron repressor DesR, 230 aa. Contains Pfam match to entry PF01325 Fe_dep_repress, Iron dependent repressor. Contains also a possible helix-turn-helix motif at residues 25..46 (+2.65 SD) 1 2 1 7 [Reporter]SCO1182 (15E11)_PCR/2022 [Gene]SCO1182/1841 NC_003888 SCG11A.13, hypothetical protein, len: 1726 aa; similar to TR:CAB46975 (EMBL:AL096825) Streptomyces coelicolor hypothetical 181.5 KD protein SC6G3.03, 1678 aa; fasta scores: opt: 690 z-score: 716.6 E(): 1.5e-32; 35.6% identity in 1800 aa overlap 1 2 1 6 [Reporter]SCO3734 (16A11)_PCR/2021 [Gene]SCO3734/1840 NC_003888 SCH22A.12c, hypothetical protein, len: 117 aa; similar to TR:AAF09825 (EMBL:AE001885) Deinococcus radiodurans conserved hypothetical protein DR0242, 112 aa; fasta scores: opt: 128 z-score: 175.1 E(): 0.025; 32.7% identity in 110 aa overlap 4 4 13 20 [Reporter]SCO5897 (3L9)_PCR/7842 [Gene]SCO5897/7139 NC_003888 SC10A5.02, redG, probable oxidase, len: 395 aa; similar in N- terminus to many diverse oxidases e.g. TR:P95483 (EMBL:U7449 3) aminopyrrolnitrin oxidase PrnD from Pseudomonas fluorescens (363 aa), fasta scores; opt: 349 z-score: 372.1 E(): 1. 6e-13, 33.3% identity in 165 aa overlap 4 4 13 19 [Reporter]SCO5545 (4H9)_PCR/7841 [Gene]SCO5545/7138 NC_003888 SC1C2.26, unknown, len: 58 aa 1 2 1 5 [Reporter]SCO1617 (17M7)_PCR/2020 [Gene]SCO1617/1839 NC_003888 SCI35.39, probable integral membrane protein, len: 394 aa; similar to several hypothetical membrane proteins e.g. Rhodococcus erythropolis TR:O07448 (EMBL:AF002247) (295 aa), fasta scores; opt: 868 z-score: 961.0 E(): 0, 44.9% identity in 292 aa overlap 4 4 13 18 [Reporter]SCO6401 (5D9)_PCR/7840 [Gene]SCO6401/7137 NC_003888 SC3C8.21c, unknown ORF in IS117, len: 175 aa; identical to, but longer than, YM2_STRCO mini-circle hypothetical 13.3 KD protein (122 aa). Note alternative ORF on opposite strand. Contains TTA Leu codon; possible site for action of bldA 1 2 1 4 [Reporter]SCO3370 (18I7)_PCR/2019 [Gene]SCO3370/1838 NC_003888 SCE94.21, possible large ATP-binding protein, len: 861 aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). Similar to two closely neighbouring genes SCE94.23 and SCE94.20 suggesting local duplication; fasta scores: SCE94.23 opt: 984, z-score: 883.6, E(): 0, (43.6% identity in 857 aa overlap), SCE94.20 opt: 789, z-score: 709.4, E(): 1.4e-34, (44.7% identity in 877 aa overlap) 4 4 13 17 [Reporter]SCO5816 (6P5)_PCR/7839 [Gene]SCO5816/7136 NC_003888 SC5B8.06, unknown, len: 534 aa. Contains Probable coiled-coil from 234 to 270 4 1 23 22 [Reporter]SCO4920 (1L24)_PCR/2016 [Gene]SCO4920/1837 NC_003888 SCK13.12, probable deoR-family transcriptional regulator, len: 317 aa; similar to SW:GATR_ECOLI (EMBL:AE000298) Escherichia coli galactitol utilization operon repressor GatR, 259 aa; fasta scores: opt: 442 z-score: 423.7 E(): 4.6e-16; 34.6% identity in 237 aa overlap. Contains Pfam match to entry PF00455 deoR, Bacterial regulatory proteins, deoR family and match to Prosite entry PS00894 Bacterial regulatory proteins, deoR family signature. Also contains possible helix-turn-helix motif at residues 8..29 (+4.06 SD) 4 4 13 16 [Reporter]SCO5432 (7L5)_PCR/7838 [Gene]SCO5432/7135 NC_003888 SC6A11.08, possible integral membrane protein, len: 179 aa; no significant database similarities. Contains possible membrane-spanning hydrophobic regions 4 1 23 21 [Reporter]SCO5514 (2H24)_PCR/2015 [Gene]SCO5514/1836 NC_003888 SC8D9.26, ilvC, acetohydroxy acid isomeroreductase, len: 174 aa; high level of similarity to many e.g. TR:Q59818 (EMBL:L39268) IlvC, acetohydroxy acid isomeroreductase from the ilvBNC gene cluster of Streptomyces avermitilis (333 aa) fasta scores; opt: 2075, z-score: 2404.5, E(): 0, (94.6% identity in 333 aa overlap). 4 4 13 15 [Reporter]SCO4747 (8H5)_PCR/7837 [Gene]SCO4747/7134 NC_003888 SC6G4.25, probable ATP/GTP binding protein, len: 148 aa; similar to hypothetical proteins from many organisms e .g. YJEE_ECOLI (153 aa), fasta scores; opt: 228 z-score: 29 2.3 E(): 5.1e-09, 34.1% identity in 135 aa overlap and Y097 _MYCTU MTCY78.07 (168 aa), fasta scores; opt: 377 z-score: 532.7 E(): 2.1e-22, 50.8% identity in 120 aa overlap. Conta ins PS00017 ATP/GTP-binding site motif A (P-loop) 4 1 23 20 [Reporter]SCO6331 (3D24)_PCR/2014 [Gene]SCO6331/1835 NC_003888 SC10H5.07, hypothetical protein, len: 469 aa; similar to TR:Q9XA23 (EMBL:AL079308) Streptomyces coelicolor hypothetical protein SCH69.11c, 509 aa; fasta scores: opt: 648 Z-score: 735.0 E(): 2.6e-33; 41.614% identityin 471 aa overlap 4 4 13 14 [Reporter]SCO7723 (9D5)_PCR/7836 [Gene]SCO7723/7133 NC_003888 SC8D11.14c, unknown, len: 393 aa 4 1 23 18 [Reporter]SCO7043 (5L20)_PCR/2012 [Gene]SCO7043/1834 NC_003888 SC4G1.09, unknown, len: 110 aa. Highly hydrophilic 4 4 13 13 [Reporter]SCO6254 (10P1)_PCR/7835 [Gene]SCO6254/7132 NC_003888 SCAH10.19, possible two-component system response regulator, 229 aa; similar to TR:Q53894 (EMBL:U51332) Streptomyces coelicolor AbsA2, 222 aa; fasta scores: opt: 573 z-score: 662.7 E(): 1.5e-29; 45.7% identity in 221 aa overlap, to SW:NARL_ECOLI (EMBL:X13360) Escherichia coli nitrate/nitrite response regulator protein 216 aa; fasta scores: opt: 457 z-score: 531.1 E(): 3.3e-22; 36.1% identity in 208 aa overlap and to TR:CAB40929 (EMBL:SCE126) Streptomyces coelicolor SCE126.07c, 224 aa; fasta scores: opt: 664 z-score: 584.6 E(): 3.1e-27; 48.4% identity in 219 aa overlap. Contains two matches to Pfam entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family 4 1 23 17 [Reporter]SCO7575 (6H20)_PCR/2011 [Gene]SCO7575/1833 NC_003888 SC5F1.29c, probable secreted hydrolase, len: 285 aa; similar to SW:GUB_RHOMR (EMBL:U04836) Rhodothermus marinus beta-glucanase precursor (EC 3.2.1.73) BglA, 286 aa; fasta scores: opt: 626 z-score: 709.7 E(): 5.3e-32; 40.5% identity in 296 aa overlap and to N-terminal region of TR:CAC14352 (EMBL:AL445945) Streptomyces coelicolor putative secreted hydrolase 3SCF60.19, 422 aa; fasta scores: opt: 1508 z-score: 1618.5 E(): 0; 75.9% identity in 299 aa overlap. Contains Pfam match to entry PF00722 Glyco_hydro_16, Glycosyl hydrolases family 16 and match to Prosite entry PS01034 Glycosyl hydrolases family 16 active sites. Also contains possible N-terminal region signal peptide sequence 4 4 13 12 [Reporter]SCO2348 (11L1)_PCR/7834 [Gene]SCO2348/7131 NC_003888 SCC8A.06, putative secreted protein, len: 214 aa; similar to TR:P95123 (EMBL:Z83018) hypothetical protein from Mycobacterium tuberculosis (237 aa) fasta scores; opt: 335, z-score: 412.6, E(): 1.6e-15, 35.4% identity in 223 aa overlap. Contains possible N-terminal region signal peptide sequence 4 1 23 16 [Reporter]SCO7411 (7D20)_PCR/2010 [Gene]SCO7411/1832 NC_003888 SC6D11.07, possible LacI-family transcriptional regulator, len: 352 aa. Similar to several including Escherichia coli SW:LACI_ECOLI (EMBL:V00294) lactose operon repressor LacI (360 aa), fasta scores opt: 595 z-score: 670.0 E(): 6.7e-30 34.8% identity in 333 aa overlap and Streptomyces coelicolor TR:O86618 (EMBL:AL031155) putative transcriptional regulator SC3A7.17 (361 aa), fasta scores opt: 887 z-score: 995.3 E(): 0 46.0% identity in 350 aa overlap. Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family. Possesses a putative helix-turn-helix motif situated between residues 23..44. 4 4 13 11 [Reporter]SCO4452 (12H1)_PCR/7833 [Gene]SCO4452/7130 NC_003888 SCD6.30c, hypothetical protein, len: 383 aa; similar to TR:O86798 (EMBL:AL031317) Streptomyces coelicolor hypothetical 45.7 kD protein SC6G4.35, 419 aa; fasta scores: opt: 312 z-score: 358.2 E(): 1.7e-12; 42.3% identity in 404 aa overlap and to many putative RNA polymerase sigma facotrs, e.g. TR:Q984U6 (EMBL:AP003012) Rhizobium loti (Mesorhizobium loti) sigma-70 factor, ECF subfamily MLL7837 417 aa; fasta scores: opt: 535 Z-score: 594.4 E(): 1.8e-25; 43.325% identity in 397 aa overlap 4 1 23 15 [Reporter]SCO2013 (8P16)_PCR/2009 [Gene]SCO2013/1831 NC_003888 SC7H2.27c, probable two-component system response regulator, len: 218aa; similar to many eg. TR:Q53894 (EMBL:U51332) AbsA2 response regulator from Streptomyces coelicolor (222 aa) fasta scores; opt: 313, z-score: 359.0, E(): 1.1e-12, (36.1% identity in 155 aa overlap). Also similar to TR:O06143 (EMBL:Z95554) hypothetical protein from Mycobacterium tuberculosis (205 aa) fasta scores; opt: 880, z-score: 986.3, E(): 0, (69.4% identity in 196 aa overlap). Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain. 4 1 23 14 [Reporter]SCO6124 (9L16)_PCR/2008 [Gene]SCO6124/1830 NC_003888 SC9B2.11, unknown, len: 109 aa; has a short region of similarity to C-terminal region of SW:TRPD_MYCTU probable anthranilate phosphoribosyltransferase (370 aa) fasta scores; opt: 111, z-score: 142.1, E(): 1.3, (38.5% identity in 65 aa overlap). 4 4 13 10 [Reporter]SCO2853 (13D1)_PCR/7832 [Gene]SCO2853/7129 NC_003888 SCE20.27c, unknown, len: 184 aa. Similar to many proteins of unknown function including: Mycobacterium tuberculosis SW:YU95_MYCTU(EMBL:Z95150) hypothetical 17.8 KD protein (158 aa), fasta scores opt: 396 z-score: 478.9 E(): 2.7e-19 42.6% identity in 141 aa overlap and Streptomyces coelicolor TR:O86588(EMBL:AL031514) hypothetical 23.3 KD protein SC2H4.05 (216 aa), fasta scores opt: 294 z-score: 357.1 E(): 1.7e-12 44.6% identity in 112 aa overlap. Contains a Pfam match to entry PF01638 DUF24, Protein of unknown function 4 4 13 9 [Reporter]SCO3351 (13P21)_PCR/7831 [Gene]SCO3351/7128 NC_003888 SCE94.02, possible DNA repair protein, len: 469aa; similar to many eg. SW:RADA_ECOLI RadA, DNA repair protein (mechanism unknown) from Escherichia coli (460 aa) fasta scores; opt: 1218, z-score: 1309.3, E(): 0, (41.4% identity in 452 aa overlap). Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 1 23 12 [Reporter]SCO4347 (11D16)_PCR/2006 [Gene]SCO4347/1829 NC_003888 SCD19.02c, unknown, len: 142 aa. High content in alanine, arginine and leucine amino acid residues 4 4 13 8 [Reporter]SCO0696 (14L21)_PCR/7830 [Gene]SCO0696/7127 NC_003888 SCF42.06c, possible integral membrane protein, len: 164 aa. Contains multiple possible membrane spanning hydrophobic domains. 4 1 23 11 [Reporter]SCO4230 (12P12)_PCR/2005 [Gene]SCO4230/1828 NC_003888 SCD8A.03, probable response regulator, len: 223 aa; similar to TR:O86269 (EMBL:AJ001103) Lactococcus lactis ArcA protein, 233 aa; fasta scores: opt: 700 z-score: 810.0 E(): 0; 49.3% identity in 223 aa overlap and to TR:Q56180 (EMBL:U38917) Synechococcus sp. response regulator PhoB, 234 aa; fasta scores: opt: 687 z-score: 795.1 E(): 0; 48.0% identity in 229 aa overlap, and similar to many S. coelicolor response regulators, e.g. SCH22A.19c, 256 aa; fasta scores: opt: 687 z-score: 696.9 E(): 2.5e-33; 49.1% identity in 224 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 4 4 13 7 [Reporter]SCO0439 (15H21)_PCR/7829 [Gene]SCO0439/7126 NC_003888 SCF51A.17c, possible membrane protein, len: 324 aa. Contains a possible N-terminal signal sequence and multiple possible membrane spanning hydrophobic domains. 4 1 23 10 [Reporter]SCO3454 (13L12)_PCR/2004 [Gene]SCO3454/1827 NC_003888 SCE46.11c, possible ABC-transporter membrane protein, len: 280 aa; similar to TR:Q44381 (EMBL:U60011) Agrobacterium tumefaciens inner membrane protein MotD, 273 aa; fasta scores: opt: 360 z-score: 418.0 E(): 6.1e-16; 29.3% identity in 256 aa overlap and to SW:POTC_ECOLI (EMBL:M64519) Escherichia coli spermidine/putrescine transport system permease protein PotC, 264 aa; fasta scores: opt: 309 z-score: 360.4 E(): 1e-12; 25.8% identity in 260 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and Prosite match to entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and possible N-terminal region signal peptide sequence. Contains also a possible helix-turn-helix motif at residues 159..180 (+3.23 SD) 4 4 13 6 [Reporter]SCO1619 (16D21)_PCR/7828 [Gene]SCO1619/7125 NC_003888 SCI41.02c, possible transcriptional regulatory protein, len: 189 aa; similarity with TR:O53759 (EMBL:AL021933) Mycobacterium tuberculosis putative regulatory protein (140 aa); fasta scores: opt: 164 z-score: 208.0 E(): 0.00032, 31.3% identity in 112 aa overlap. Contains Pfam match PF01381 HTH_3, Helix-turn-helix. Contains probable helix-turn-helix motif at aa 25-46 (Score 1293, +3.59 SD) 4 1 23 9 [Reporter]SCO0484 (14H12)_PCR/2003 [Gene]SCO0484/1826 NC_003888 SCF34.03c, possible monooxygenase, len: 388 aa; similar to SW:TETX_BACFR (EMBL:M37699), TetX, Bacteroides fragilis tetracycline resistance protein from transposon Tn4351/Tn4400 (388 aa), fasta scores; opt: 582 z-score: 637.6 E(): 3.7e-28, 33.9% identity in 351 aa overlap. Also similar to monooxygenases e.g. TR:P96555 (EMBL:AB000564) Sphingomonas sp. salicylate hydroxylase (395 aa) (27.2% identity in 360 aa overlap). Similar to others from S.coelicolor e.g. TR:Q9Z4Y6 (EMBL:AL035707) S.coelicolor possible salicylate hydroxylase (420 aa) (32.9% identity in 340 aa overlap). Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase 4 4 13 5 [Reporter]SCO1768 (17P17)_PCR/7827 [Gene]SCO1768/7124 NC_003888 SCI51.08c, possible pseudouridine synthase, len: 371 aa; part of this CDS shows similarity to SW:RSUA_ECOLI (EMBL:U00008), rsuA, Escherichia coli ribosomal small subunit pseudouridine synthase A (231 aa), fasta scores; opt: 389 z-score: 356.8 E(): 1.6e-12, 35.2% identity in 233 aa overlap and to many putative pseudouridine synthases. Similarity to SW:YRSU_MYCLE (EMBL:Z95117) Mycobacterium leprae hypothetical protein (256 aa) (54.6% identity in 240 aa overlap) and its Mycobacterium tuberculosis counterpart is high. GC frameplot and an absence of possible start codons suggests that an N-terminal extension of approx. 130 aa is present in this sequence, but not in similar sequences. The N-terminus is Ser- and Gly-rich. Contains Pfam matches to entry PF01479 S4, S4 domain and to entry PF00849 YABO, Hypothetical yabO/yceC/sfhB family. Contains PS01149 RsuA family of pseudouridine synthase signature 4 1 23 8 [Reporter]SCO0529 (15D12)_PCR/2002 [Gene]SCO0529/1825 NC_003888 SCF11.09c, probable oxidoreductase (putative secreted protein), len: 508 aa; similar to TR:P75002 (EMBL:Z80356) Zymomonas mobilis glucose-fructose oxidoreductase precursor, 433 aa; fasta scores: opt: 270 z-score: 301.6 E(): 2e-09; 28.8% identity in 219 aa overlap. Contains a Pfam match to entry PF01408 GFO_IDH_MocA, oxidoreductase family. Contains possible n-terminal region signal sequence peptide 4 4 13 4 [Reporter]SCP1.222 (18L17)_PCR/7826 [Gene]SCP1.222/7123 NC_003888 SCP1.222, parB2, possible plasmid partitioning protein, len: 381aa; weakly similar to many eg. SW:P26497 (SP0J_BACSU) Spo0J protein required for initiation of sporulation and normal chromosome segregation in Bacillus subtilis (282 aa) fasta scores; opt: 213, z-score: 210.9, E(): 5.3e-05, 30.2% identity in 212 aa overlap. Contains Pfam match to entry PF02195 ParBc, ParB-like nuclease domain. This CDS is directly duplicated at 130087..131214 (ParB1, SCP1.139) fasta scores; opt: 566, z-score: 538.4, E(): 1.8e-25, 33.6% identity in 384 aa overlap. 4 1 23 7 [Reporter]SCO3847 (16P8)_PCR/2001 [Gene]SCO3847/1824 NC_003888 SCH69.17, probable secreted penicillin-binding protein, len: 490 aa; similar to many e.g. TR:P71586 (EMBL:Z80233) putative penicillin-binding protein from Mycobacterium tuberculosis (491 aa) fasta scores; opt: 112,1 z-score: 1262.1, E(): 0, (39.3% identity in 499 aa overlap) and SW:PBPC_BACSU penicillin-binding protein 3 from Bacillus subtilis (668 aa) fasta scores; opt: 447, z-score: 502.5, E(): 1.2e-20, (29.0% identity in 386 aa overlap). Also similar to SC6G9.32 from Streptomyces coelicolor (485 aa) fasta scores; opt: 1493, z-score: 1531.4, E(): 0, (49.4% identity in 488 aa overlap). Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain. Also contains possible N-terminal signal sequence. 4 4 13 3 [Reporter]SCO4024 (19H17)_PCR/7825 [Gene]SCO4024/7122 NC_003888 2SC10A7.28, probable integral membrane efflux protein, len: 507 aa; highly similar to TR:Q54806 (EMBL:X84072) Streptomyces pristinaespiralis integral membrane protein Ptr, 501 aa; fasta scores: opt: 2316 z-score: 2400.3 E(): 0; 72.8% identity in 496 aa overlap and to TR:Q9XE01 (EMBL:AB019519) virginiamycin S resistance protein from Streptomyces virginiae (518 aa) fasta scores; opt: 1972, Z-score: 1995.4, 62.828% identity (63.992% ungapped) in 495 aa overlap. Contains matches to Prosite entries PS00216 Sugar transport proteins signature 1 and PS00012 Phosphopantetheine attachment site. Also contains possible hydrophobic membrane spanning region 4 1 23 6 [Reporter]SCO1560 (17L8)_PCR/2000 [Gene]SCO1560/1823 NC_003888 SCL11.16c, possible phosphatase, len: 216 aa; similar to TR:P96428 (EMBL:Z79692) Sinorhizobium meliloti ORF24, 233 aa; fasta scores: opt: 488 z-score: 573.6 E(): 1.6e-24; 42.2% identity in 206 aa overlap and to TR:BAA16133 (EMBL:D90861)Escherichia coli 2-deoxyglucose-6-phosphate phosphatase 1 (EC 3.1.3.-) Dog1, 222aa; fasta scores: opt: 430 z-score: 507.0 E(): 8.1e-21; 36.2% identity in 213 aa overlap 4 4 13 2 [Reporter]SCO3996 (20P13)_PCR/7824 [Gene]SCO3996/7121 NC_003888 SCBAC25E3.33, possible excisionase, len: 69 aa: similar to many eg. SW:P15482 (VXIS_STRAM) excisionase Xis from Streptomyces ambofaciens plasmid pSAM2 (62 aa) fasta scores; opt: 93, Z-score: 151.3, 34.545% identity (34.545% ungapped) in 55 aa overlap. 4 1 23 4 [Reporter]SCP1.139 (19D8)_PCR/1998 [Gene]SCP1.139/1822 NC_003888 SCP1.139, parB1, plasmid probable partitioning protein, len: 375aa; weakly similar to many ParB-like proteins eg. TR:Q9RFM2 (EMBL:AF187159) ParB partitioning protein from Streptomyces coelicolor (368 aa) fasta scores; opt: 176, z-score: 188.6, E(): 0.0049, 32.3% identity in 192 aa overlap. Contains Pfam match to entry PF02195 ParBc, ParB-like nuclease domain. This CDS is directly duplicated at 130087..131214 (ParB2, SCP1.222) fasta scores; opt: 566, z-score: 524.0 E(): 1.2e-24, 33.6% identity in 384 aa overlap. 4 4 12 22 [Reporter]SCO1251 (1P9)_PCR/7822 [Gene]SCO1251/7120 NC_003888 2SCG1.26, possible integral membrane protein, len: 468 aa; similar to TR:Q9RKZ4 (EMBL:AL133213) Streptomyces coelicolor putative integral membrane protein SC6D7.03, 443 aa; fasta scores: opt: 1577 z-score: 1678.6 E(): 0; 59.3% identity in 427 aa overlap. Contains Pfam matches to entries PF01595 DUF21, Domain of unknown function DUF21 and 2x PF00571 CBS, CBS domain. Also contains possible N-terminal region signal peptide sequence (seems to be cleavable) and possible hydrophobic membrane spanning regions 4 1 23 3 [Reporter]SCO6080 (20P4)_PCR/1997 [Gene]SCO6080/1821 NC_003888 SCBAC1A6.04, possible dehydrogenase, len: 385 aa; similar to TR:Q53924 (EMBL:X62373) Streptomyces coelicolor hydroxylacyl-CoA dehydrogenase ORFB, 394 aa; fasta scores: opt: 835 z-score: 981.3 E(): 0; 42.0% identity in 381 aa overlap 4 1 22 22 [Reporter]SCO1103 (1H24)_PCR/1995 [Gene]SCO1103/1820 NC_003888 2SCG4.19, probable hydrolase, len: 229 aa; similar to SW:HAD4_BURCE (EMBL:X66249) Burkholderia cepacia 2-haloalkanoic acid dehalogenase IVa (EC 3.8.1.2) Hdl IVa, 230 aa; fasta scores: opt: 218 z-score: 257.2 E(): 7.6e-07; 29.8% identity in 205 aa overlap. Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase 4 4 12 21 [Reporter]SCO5744 (2L9)_PCR/7821 [Gene]SCO5744/7119 NC_003888 SC9A10.08, dapA, dihydrodipicolinate synthase, len: 299 aa; highly similar to many e.g. DAPA_CORGL dihydrodipicolinate synthase (EC 4.2.1.52) (301 aa), fasta scores; opt: 957 z-score: 1290.5 E(): 0, 53.3% identity in 287 aa overlap. Contains PS00665 Dihydrodipicolinate synthetase signature 1 and Pfam match to entry PF00701 DHDPS, Dihydrodipicolinate synthetase, score 351.50, E-value 9.2e-102 4 4 12 20 [Reporter]SCO4068 (3H9)_PCR/7820 [Gene]SCO4068/7118 NC_003888 SCD25.04, purD, phosphoribosylamine-glycine ligase (EC 6.3.4.13), len: 416 aa. Highly similar to many e.g. Mycobacterium tuberculosis SW:PUR2_MYCTU (EMBL; Z80226) phosphoribosylamine--glycine ligase (EC 6.3.4.13) (GARS) (glycinamide ribonucleotide synthetase) (phosphoribosylglycinamide synthetase) MTCY369.17 (422 aa), fasta scores opt: 957 z-score: 1033.0 E(): 0 58.3% identity in 424 aa overlap. Contains a PS00184 Phosphoribosylglycinamide synthetase signature and a Pfam match to entry PF01071 GARS, Phosphoribosylglycinamide synthetase (GARS). 4 1 22 21 [Reporter]SCO4932 (2D24)_PCR/1994 [Gene]SCO4932/1819 NC_003888 SCK13.24, hutH, histidine ammonia-lyase, len: 512 aa; highly similar to SW:HUTH_STRGR (EMBL:M77841) Streptomyces griseus histidine ammonia-lyase (EC 4.3.1.3) HutH, 516 aa; fasta scores: opt: 2928 z-score: 3294.6 E(): 0; 88.5% identity in 513 aa overlap. Contains Pfam match to entry PF00221 PAL, Phenylalanine and histidine ammonia-lyases and matches to Prosite entries PS00217 Sugar transport proteins signature 2 and PS00488 Phenylalanine and histidine ammonia-lyases signature 4 4 12 19 [Reporter]SCO5538 (4D9)_PCR/7819 [Gene]SCO5538/7117 NC_003888 SC1C2.19c, cvnC2, unknown, len: 190 aa; N-terminus is Pro-rich, C-terminus has similarity to M. tuberculosis hypothetical protein TR:O50392 (EMBL:AL009198) MTV004.20C (122 aa), fasta scores; opt: 231 z-score: 217.7 E(): 7.1e-05, 41.0% identity in 122 aa overlap. C-terminus is 84.7% identical to upstream gene SC3A9.23c 4 1 22 20 [Reporter]SCO1389 (3P20)_PCR/1993 [Gene]SCO1389/1818 NC_003888 SC1A8A.09c, possible CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase, len: 206 aa; similar to many eg. SW:Q50611 (PGSA_MYCTU) putative CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase from Mycobacterium tuberculosis (209 aa) fasta scores; opt: 504, z-score: 637.7, E(): 4.3e-28, 43.5% identity in 193 aa overlap and SW:P06978 (PGSA_ECOLI ) CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase from Escherichia coli (181 aa) fasta scores; opt: 232, z-score: 299.4 E(): 3e-09, 29.3% identity in 174 aa overlap. Contains Pfam match to entry PF01066 CDP-OH_P_transf, CDP-alcohol phosphatidyltransferase and Prosite match to PS00379 CDP-alcohol phosphatidyltransferases signature. Contains possible hydrophobic membrane spanning region 4 4 12 18 [Reporter]SCO6579 (5P5)_PCR/7818 [Gene]SCO6579/7116 NC_003888 SC3F9.14, unknown, len: 465 aa, similar to TR:Q9Z4Z4 (EMBL:AL035707) hypothetical protein from Streptomyces coelicolor (462 aa) fasta scores; opt: 2206, Z-score: 2466.5, 70.575% identity (70.889% ungapped) in 452 aa overlap. 4 1 22 19 [Reporter]SCO7628 (4L20)_PCR/1992 [Gene]SCO7628/1817 NC_003888 SC10F4.01, conserved hypothetical protein (fragment), len: >90 aa; similar to TR:O52799 (EMBL:AJ223998) Amycolatopsis orientalis hypothetical protein PCZA361.11, 495 aa; fasta scores: opt: 151 z-score: 192.7 E(): 0.0034; 35.4% identity in 79 aa overlap,SC2H2.26, conserved hypothetical protein (fragment), len: >322 aa; similar to TR:O52799 (EMBL:AJ223998) Amycolatopsis orientalis hypothetical protein PCZA361.11, 495 aa; fasta scores: opt: 757 z-score: 813.7 E(): 0; 45.0% identity in 333 aa overlap. Contains possible N-terminal region signal peptide sequence 4 4 12 17 [Reporter]SCO6682 (6L5)_PCR/7817 [Gene]SCO6682/7115 NC_003888 SC5A7.32, unknown, len: 42 aa; similar to CDS upstream of S. griseus RamA TR:Q07642 (EMBL:D13614) (43 aa), fasta scores; opt: 154 z-score: 293.0 E(): 4.4e-09, 53.7% identity in 41 aa overlap 4 1 22 18 [Reporter]SCO7114 (5H20)_PCR/1991 [Gene]SCO7114/1816 NC_003888 SC4B10.15c, conserved hypothetical protein, len: 463 aa; similar to TR:Q9ZN74 (EMBL:AB022095) Streptomyces griseus ORF3 protein, 456 aa; fasta scores: opt: 828 z-score: 929.7 E(): 0; 38.5% identity in 470 aa overlap 4 4 12 16 [Reporter]SCO7279 (7H5)_PCR/7816 [Gene]SCO7279/7114 NC_003888 SC5H1.13c, possible DNA-binding protein, len: 157 aa; contains region similar to TR:O33287 (EMBL:AL008967) Mycobacterium tuberculosis possible transcriptional regulator (112 aa), fasta scores; opt: 254 z-score: 260.9 E(): 3.3e-07, 50.0% identity in 94 aa overlap and TR:O86815 (EMBL:AL031031), SC7C7.10, S.coelicolor hypothetical protein (126 aa) (55.6% identity in 72 aa overlap). Possible DNA-binding function as it contains probable helix-turn-helix motif at aa 77-98 (Score 1354, +3.80 SD) and Pfam match to entry PF01381 HTH_3, Helix-turn-helix, score 41.30, E-value 2.2e-08. PQATR repeat present in proline-rich N-terminus 4 1 22 17 [Reporter]SCO7570 (6D20)_PCR/1990 [Gene]SCO7570/1815 NC_003888 SC5F1.24, probable isomerase, len: 375 aa; similar to SW:GUDH_STRCO (EMBL:AL136503) Streptomyces coelicolor probable glucarate dehydratase (EC 4.2.1.40) GudD or SCC77.09c, 431 aa; fasta scores: opt: 553 z-score: 623.1 E(): 3.6e-27; 37.2% identity in 398 aa overlap and to SW:TFDD_ALCEU (EMBL:M35097) Alcaligenes eutrophu chloromuconate cycloisomerase (EC 5.5.1.7) TfdD, 370 aa; fasta scores: opt: 419 z-score: 474.3 E(): 7e-19; 29.1% identity in 385 aa overlap. Contains Pfam match to entry PF01188 MR_MLE, Mandelate racemase / muconate lactonizing enzyme family. Contains possible hydrophobic membrane spanning region 4 4 12 14 [Reporter]SCO2647 (9P1)_PCR/7814 [Gene]SCO2647/7113 NC_003888 SC8E4A.17, probable MarR-family regulatory protein, len: 172 aa; similar to SW:PECS_ERWCH (EMBL:X74409) Erwinia chrysanthemi regulatory protein PecS, 166 aa; fasta scores: opt: 372 z-score: 442.3 E(): 3.2e-17; 39.6% identity in 149 aa overlap and to SW:MARR_SALTY (EMBL:U54468) Salmonella typhimurium multiple antibiotic resistance protein MarR, 144 aa; fasta scores: opt: 181 z-score: 223.4 E(): 5e-0; 28.6% identity in 133 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 4 1 22 16 [Reporter]SCO2117 (7P16)_PCR/1989 [Gene]SCO2117/1814 NC_003888 SC6E10.11, trpE1, probable anthranilate synthase, len: 634 aa; highly similar to many anthranilate synthases e.g. TR:O30589 (EMBL:AF012627), TrpE, Streptomyces venezuelae anthranilate synthase (623 aa), fasta scores; opt: 3108 z-score: 3257.7 E(): 0, 80.0% identity in 620 aa overlap. Anthranilate synthases may have one or two components. This protein is similar at the C-terminus to TR:Q9Z4W8 (EMBL:AL035654), TrpG, S.coelicolor probable anthranilate synthase component II (200 aa) (32.7% identity in 202 aa overlap) and centrally to TR:Q9Z4W7 (EMBL:AL035654), trpE2, S.coelicolor probable anthranilate synthase component I (511 aa) (30.6% identity in 301 aa overlap) which appear to constitute a two-component type enzyme. Contains two Pfam matches to entry PF00425 chorismate_bind, chorismate binding enzyme and Pfam match to entry PF00117 GATase, Glutamine amidotransferase class-I 4 4 12 13 [Reporter]SCO6250 (10L1)_PCR/7813 [Gene]SCO6250/7112 NC_003888 SCAH10.15, probable transport protein, len: 197 aa; similar to C-terminus of TR:CAB55652 (EMBL:AL117385) Streptomyces coelicolor putative transmembrane efflux protein SC5G9.04c 489 aa; fasta scores: opt: 230 z-score: 254.1 E(): 8.8e-07; 33.5% identity in 185 aa overlap and to C-terminus of SW:PUR8_STRLP (EMBL:X76855) Streptomyces lipmanii (Streptomyces alboniger) puromycin resistance protein, Pur8, 503 aa; fasta scores: opt: 237 z-score: 261.4 E(): 3.5e-07; 30.2% identity in 162 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 22 15 [Reporter]SCO2009 (8L16)_PCR/1988 [Gene]SCO2009/1813 NC_003888 SC7H2.23, probable branched chain amino acid transport permease, len: 310aa; similar to many eg. SW:LIVH_SALTY high affinity branched chain amino acid transport permease from Salmonella typhimurium (308 aa) fasta scores; opt: 711, z-score: 782.4, E(): 0, (43.5% identity in 306 aa overlap). Contains possible membrane spanning hydrophobic regions. 4 4 12 11 [Reporter]SCO4448 (12D1)_PCR/7811 [Gene]SCO4448/7111 NC_003888 SCD6.26c, unknown, len: 227 aa 4 1 22 13 [Reporter]SCO2441 (10D16)_PCR/1986 [Gene]SCO2441/1812 NC_003888 SCC24.12, unknown, len: 200 aa. High content in alanine and arginine residues 4 4 12 10 [Reporter]SCO3533 (12P21)_PCR/7810 [Gene]SCO3533/7110 NC_003888 SCE2.14, unknown, len: 221aa; 4 1 22 12 [Reporter]SCO4557 (11P12)_PCR/1985 [Gene]SCO4557/1811 NC_003888 SCD16A.26, unknown, len: 115aa; 4 1 22 11 [Reporter]SCO4267 (12L12)_PCR/1984 [Gene]SCO4267/1810 NC_003888 SCD86A.04c, hypothetical protein, len: 386 aa; similar to TR:O06003 (EMBL:X86552) Bradyrhizobium japonicum hypothetical 46.6 kD protein, 439 aa; fasta scores: opt: 153 z-score: 175.4 E(): 0.027; 31.3% identity in 201 aa overlap 4 4 12 9 [Reporter]SCO1454 (13L21)_PCR/7809 [Gene]SCO1454/7109 NC_003888 SCL6.11c, possible amino oxidase, len: 565 aa; similar to C-terminal region of SW:TR2M_AGRVI (EMBL:M91609) Agrobacterium vitis tryptophan 2-monooxygenase (EC 1.13.12.3) IaaM (auxins biosynthesis), 723 aa; fasta scores: opt: 617 z-score: 725.3 E(): 5.6e-33; 30.9% identity in 557 aa overlap. Contains Pfam match to entry PF01593 Amino_oxidase, Flavin containing amine oxidase 4 4 12 8 [Reporter]SCO1169 (14H21)_PCR/7808 [Gene]SCO1169/7108 NC_003888 2SCG11.03c, xylA, xylose isomerase, len: 387 aa; identical to SW:XYLA_STRRU (EMBL:M73789) Streptomyces rubiginosus xylose isomerase XylA, 387 aa. Contains Pfam match to entry PF00259 Xylose_isom, Xylose isomerase and matches to Prosite entries PS00172 Xylose isomerase signature 1 and PS00173 Xylose isomerase signature 2 4 1 22 10 [Reporter]SCO3017 (13H12)_PCR/1983 [Gene]SCO3017/1809 NC_003888 SCE33.19, possible secreted protein, len: 387 aa; similar to TR:O69659 (EMBL::AL022121) Mycobacterium tuberculosis hypothetical 355 kD protein MTV025.039, 333 aa; fasta scores: opt: 481 z-score: 542.0 E(): 9.9e-23; 36.6% identity in 358 aa overlap. Contains possible N-terminal region signal peptide sequence 4 4 12 7 [Reporter]SCO4809 (15D21)_PCR/7807 [Gene]SCO4809/7107 NC_003888 SCD63A.20, sucD, succinyl CoA synthetase alpha chain, len: 294 aa; similar to SW:SUCD_ECOLI (EMBL:J01619) Escherichia coli succinyl CoA synthetase alpha chain (EC 6.2.1.5) SucD, 288 aa; fasta scores: opt: 986 z-score: 1029.6 E(): 0; 53.6% identity in 293 aa overlap and to TR:O87841 (EMBL:AL031013) Streptomyces coelicolor succinyl CoA synthetase alpha chain SucD1 SC8A6.07, 308 aa; fasta scores: opt: 1429 z-score: 1453.2 E(): 0; 75.9% identity in 286 aa overlap. Contains Pfam match to entry PF00549 ligase-CoA, CoA-ligases and matches to Prosite entries PS01216 ATP-citrate lyase / succinyl-CoA ligases family signature, PS00399 ATP-citrate lyase / succinyl-CoA ligases family active site and PS00017 ATP/GTP-binding site motif A (P-loop) 4 1 22 8 [Reporter]SCO0618 (15P8)_PCR/1981 [Gene]SCO0618/1808 NC_003888 SCF56.02, hypothetical protein, len: 461 aa; similar to various hypothetical proteins, e.g. TR:BAA86645 (EMBL:AB033763) Staphylococcus aureus hypothetical 49.4 kD protein, 442 aa; fasta scores: opt: 286 z-score: 318.4 E(): 2.3e-10; 26.6% identity in 466 aa overlap 4 4 12 6 [Reporter]SCO0579 (16P17)_PCR/7806 [Gene]SCO0579/7106 NC_003888 SCF55.03c, possible sugar-phosphate isomerase, len: 170 aa; similar to SW:RPIB_ECOLI (EMBL:X82203) Escherichia coli ribose 5-phosphate isomerase B (EC 5.3.1.6) (phosphoriboisomerase B) RpiB, 149 aa ;fasta scores: opt: 299 z-score: 364.4 E(): 6.3e-13; 38.5% identity in 148 aa overlap 4 1 22 7 [Reporter]SCO0479 (16L8)_PCR/1980 [Gene]SCO0479/1807 NC_003888 SCF76.19c, putative phosphatase, len: 274 aa. Almost identical to Streptomyces lividans TR:O70034 (EMBL; AJ223365) Sbl1 gene (274 aa), fasta scores opt: 1764 z-score: 2005.4 E():0 97.1% identity in 274 aa overlap. Also weakly similar to many inositol monophosphatases e.g. Mycobacterium smegmatis TR:O51845 (EMBL; AF005905) inositol monophosphate phosphatase (276 aa), fasta scores opt: 299 z-score: 346.7 E(): 6e-12 33.1% identity in 269 aa overlap and Escherichia coli SW: SUHB_ECOLI (EMBL; M34828) extragenic suppressor protein SuhB (267 aa), fasta scores opt: 269 z-score: 313.0 E(): 4.6e-10 30.8% identity in 263 aa overlap. 4 4 12 5 [Reporter]SCO1615 (17L17)_PCR/7805 [Gene]SCO1615/7105 NC_003888 SCI35.37, unknown, len: 726 aa; similar to hypotheticals e.g. YCJL_ECOLI (258 aa), fasta scores; opt: 216 z-score: 265.7 E(): 1.5e-07, 31.8% identity in 201 aa overlap 4 1 22 6 [Reporter]SCO0617 (17H8)_PCR/1979 [Gene]SCO0617/1806 NC_003888 SCF56.01c, hypothetical protein (partial CDS), len: >634 aa; similar to various hypothetical proteins, e.g. TR:P74690 (EMBL:D90917) Synechocystis sp. hypothetical 92.4 kD protein, 821 aa; fasta scores: opt: 2107 z-score: 2288.7 E(): 0; 58.1% identity in 513 aa overlap,SCF55.41c, hypothetical protein, len: 210 aa; similar to various hypothetical proteins, e.g. TR:P74690 (EMBL:D90917) Synechocystis sp. (strain PCC 6803) hypothetical 92.4 KD protein, 821 aa; fasta scores: opt: 1405 z-score: 1694.1 E(): 0; 56.1% identity in 328 aa overlap 4 4 12 3 [Reporter]SCO7463 (19D17)_PCR/7803 [Gene]SCO7463/7104 NC_003888 SCBAC14E8.03, cvnA13, possible sensor histidine kinase, len: 400 aa; similar to TR:Q9L137 (EMBL:AL158061) Streptomyces coelicolor putative sensor histidine-kinase SC6D11.18c, 543 aa; fasta scores: pt: 500 Z-score: 444.9 bits: 91.6 E(): 3.3e-17; 32.591% identity in 359 aa overlap and to TR:Q9AJP4 (EMBL:AB044803) Streptomyces griseus ORF1, 543 aa; fasta scores: opt: 511 Z-score: 495.4 E(): 5.8e-20; 34.627% identity in 335 aa overlap. Contains Pfam match to entry PF02518 HATPase_c, Histidine kinase-, DNA gyrase B-, phytochrome-like ATPase and possible N-terminal region signal peptide sequence 4 1 22 5 [Reporter]SCO0904 (18D8)_PCR/1978 [Gene]SCO0904/1805 NC_003888 SCM1.37, possible secreted protein, len: 99 aa. Contains a possible N-terminal signal sequence. 4 4 12 2 [Reporter]SCO2827 (20L13)_PCR/7802 [Gene]SCO2827/7103 NC_003888 SCBAC17F8.18c, hypothetical protein, len: 167 aa: no significant database matches. 4 1 22 4 [Reporter]SCP1.08c (19P4)_PCR/1977 [Gene]SCP1.08c/1804 NC_003888 SCP1.08c, unknown, len: 173aa; 4 4 11 22 [Reporter]SCO1247 (1L9)_PCR/7800 [Gene]SCO1247/7102 NC_003888 2SCG1.22, conserved hypothetical protein, len: 225 aa; similar to TR:O06547 (EMBL:Z95584) Mycobacterium tuberculosis hypothetical 23.4 kD protein MTCI65.14, 216 aa; fasta scores: opt: 500 z-score: 599.5 E(): 6.3e-26; 45.9% identity in 209 aa overlap 4 1 22 3 [Reporter]SCO1856 (20L4)_PCR/1976 [Gene]SCO1856/1803 NC_003888 SCI39.03, probable precorrin-6Y C5,15-methyltransferase, len: 410 aa; similar to SW:COBL_PSEDE (EMBL:M59301) Pseudomonas denitrificans precorrin-6Y C5,15-methyltransferase [decarboxylating] (EC 2.1.1.132) CobL, 413 aa; fasta scores: opt: 614 Z-score: 634.0 bits: 126.3 E(): 1e-27; 37.500% identity in 408 aa overlap. Contains Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases 4 4 11 21 [Reporter]SCO4745 (2H9)_PCR/7799 [Gene]SCO4745/7101 NC_003888 SC6G4.23, alr, probable alanine racemase, len: 391 a a; similar to many e.g. ALR_BACST alanine racemase (EC 5.1. 1.1) (388 aa), fasta scores; opt: 532 z-score: 774.2 E(): 0 , 38.6% identity in 376 aa overlap. Contains PS00395 Alanine racemase pyridoxal-phosphate attachment site 4 1 21 21 [Reporter]SCO0506 (2P20)_PCR/1972 [Gene]SCO0506/1802 NC_003888 SCF6.02, nadE1, probable NH(3)-dependent NAD(+)synthetase, len: 276 aa. Highly similar to many e.g. Bacillus subtilis SW: NADE_BACSU (EMBL; M15811) NH(3)-dependent NAD(+) synthetase (EC 6.3.5.1) (spore outgrowth factor B) (sporulation protein OutB) (general stress protein 38) (Gsp38) (272 aa), fasta scores opt: 901 z-score: 1027.2 E():0 53.9% identity in 267 aa overlap. 4 4 11 20 [Reporter]SCO3125 (3D9)_PCR/7798 [Gene]SCO3125/7100 NC_003888 SCE66.04, pth, peptidyl-tRNA hydrolase (EC 3.1.1.29), len: 200aa; strongly similar to many eg. SW:P23932 (PTH_ECOLI) peptidyl-tRNA hydrolase from Escherichia coli (194 aa) fasta scores; opt: 449, z-score: 529.8, E(): 4.9e-22, 41.1% identity in 185 aa overlap. Contains Pfam match to entry PF01195 Pept_tRNA_hydro, Peptidyl-tRNA hydrolase and Prosite match to PS01196 Peptidyl-tRNA hydrolase signature 2. 4 1 21 20 [Reporter]SCO1385 (3L20)_PCR/1971 [Gene]SCO1385/1801 NC_003888 SC1A8A.05c, putative membrane protein, len: 284 aa; similar to SW:Q50608 (Y0DI_MYCTU) hypothetical protein from Mycobacterium tuberculosis (292 aa) fasta scores; opt: 421, z-score: 440.5, E(): 4.2e-17, 33.6% identity in 277 aa overlap. Contains potential coiled-coil region 106-140 aa. Contains possible hydrophobic membrane spanning region 4 1 21 19 [Reporter]SCO5866 (4H20)_PCR/1970 [Gene]SCO5866/1800 NC_003888 SC2E9.07c, putative membrane protein, len: 154 aa; similar to hypothetical proteins from Mycobacterium tuberculosis and M. leprae e.g. TR:O07200 (EMBL:Z96072) MTCY05A6.19 (161 aa), fasta scores; opt: 230 z-score: 264.3 E(): 1.6e-07, 35.7% identity in 154 aa overlap. Contains possible hydrophobic membrane spanning region 3 4 14 13 [Reporter]SCO5132 (10C5)_PCR/7353 [Gene]SCO5132/6699 NC_003888 SC9E12.17, possible two-component system response regulator, len: 204 aa; similar to TR:CAB88186 (EMBL:AL352972) Streptomyces coelicolor putative two-component response regulator SCC30.16, 210 aa; fasta scores: opt: 822 z-score: 928.1 E(): 0; 63.0% identity in 200 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family. Also contains possible helix-turn-helix motif at residues 159..180 (+5.28 SD) 3 4 14 12 [Reporter]SCO2351 (11O1)_PCR/7352 [Gene]SCO2351/6698 NC_003888 SCC8A.09, possible integral membrane protein, len: 212aa; similar to many DedA-family members eg. TR:O86706 (EMBL:AL031515) putative integral membrane protein from Streptomyces coelicolor (208 aa) fasta scores; opt: 216, z-score: 247.9, E(): 2.4e-06, 31.4% identity in 191 aa overlap and SW:P09548 (DEDA_ECOLI) DedA protein from Escherichia coli (219 aa) fasta scores; opt: 155, z-score: 181.4, E(): 0.012, 26.9% identity in 167 aa overlap. Contains possible membrane-spanning hydrophobic regions. 3 4 14 11 [Reporter]SCO4489 (12K1)_PCR/7351 [Gene]SCO4489/6697 NC_003888 SCD69.09c, putative membrane protein, len: 100 aa. Contains possible hydrophobic membrane spanning regions 3 4 14 10 [Reporter]SCO2856 (13G1)_PCR/7350 [Gene]SCO2856/6696 NC_003888 SCE20.30, unknown, len: 152 aa. Similar to another protein of unknown function from Streptomyces coelicolor TR:O86819(EMBL:AL031031) SC7C7.14 (161 aa), fasta scores opt: 152 z-score: 189.7 E(): 0.0035 31.7% identity in 123 aa overlap. 3 4 14 9 [Reporter]SCO0909 (14C1)_PCR/7349 [Gene]SCO0909/6695 NC_003888 SCM1.42c, hypothetical protein, len: 329 aa; similar to TR:Q9RZM4 (EMBL:AE001826) Deinococcus radiodurans hypothetical protein DRB0099, 285 aa; fasta scores: opt: 634 Z-score: 682.1 E(): 2.3e-30; 46.642% identity in 268 aa overlap 3 4 14 8 [Reporter]SCO3578 (14O21)_PCR/7348 [Gene]SCO3578/6694 NC_003888 SCH17.12, possible ion-transporting ATPase, len: 481 aa; similar to many putative arsenite-translocating ATPases and very weakly to TR:O54984 (EMBL:AF039405), arsA, Mus musculus arsenite-translocating ATPase (350 aa), fasta scores; opt: 192 z-score: 201.5 E(): 0.00068, 21.9% identity in 302 aa overlap. Similar to TR:O69648 (EMBL:AL022121) Mycobacterium tuberculosis putative anion transporting ATPase (386 aa) (51.2% identity in 441 aa overlap). Part of the N-terminus is weakly similar to the adjacent CDS SCH17.11 (325 aa) (25.9% identity in 158 aa overlap). Contains 3x degenerate PARHQKGX repeats near N-terminus and 3x degenerate SPEASDT repeats near the C-terminus. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 3 4 14 7 [Reporter]SCO3547 (15K21)_PCR/7347 [Gene]SCO3547/6693 NC_003888 SCH5.10c, probable pyrophosphate synthase len: 794 aa; similar to many e.g. TR:O68460 (EMBL:AF044912) H+ translocating pyrophosphate synthase from Rhodospirillum rubrum (660 aa) fasta scores; opt: 1169, z-score: 1286.1, E(): 0, (44.6% identity in 697 aa overlap). DNA sequence contains 35 base inverted repeat with 37 base loop (15201..15308). 3 4 14 6 [Reporter]SCO3869 (16G21)_PCR/7346 [Gene]SCO3869/6692 NC_003888 SCH18.06, probable WD-40 repeat protein, len: 1676 aa; C-terminal domain identical to previously sequenced TR:Q9ZEM4 (EMBL:AJ131817) Streptomyces coelicolor WD-40 repeat protein WdpA, 1049 aa; fasta scores: opt: 7025 z-score: 5917.8 E(): 0; 99.8% identity in 1049 aa overlap. Contains 13x Pfam matches to entry PF00400 WD40, WD domain, G-beta repeat, 9x matches to Prosite entry PS00678 Trp-Asp (WD) repeats signature and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 4 14 5 [Reporter]SCO0819 (17C21)_PCR/7345 [Gene]SCO0819/6691 NC_003888 SCF43A.09, possible transmembrane transport protein, len: 436 aa; probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains hydrophobic, possible membrane-spanning regions. Very weak similarity to TR:O54193 (EMBL:AL021411), cbiM, S.coelicolor probable membrane protein (452 aa), fasta scores; opt: 139 z-score: 131.9 E(): 0.033, 27.8% identity in 331 aa overlap. May be involved in membrane transport in association with its upstream neighbour which resembles an ABC-tranporter ATP-binding protein 3 4 14 4 [Reporter]SCO4890 (18O17)_PCR/7344 [Gene]SCO4890/6690 NC_003888 2SCK8.16, deoA, thymidine phosphorylase, len: 427 aa; similar to SW:TYPH_ECOLI (EMBL:U14003) Escherichia coli thymidine phosphorylase (EC 2.4.2.4) DeoA, 440 aa; fasta scores: opt: 874 z-score: 920.7 E(): 0; 41.7% identity in 436 aa overlap. Contains Pfam match to entry PF00591 Glycos_transf_3, glycosyl transferase family and match to Prosite entry PS00647 Thymidine and pyrimidine-nucleoside phosphorylases signature 3 4 14 3 [Reporter]SCO1377 (19K17)_PCR/7343 [Gene]SCO1377/6689 NC_003888 SC10A9.19, possible molibdenum binding protein, len: 139 aa; similar to C-terminal region of SW:MOPA_RHOCA (EMBL:L06254) Rhodobacter capsulatus molybdenum-pterin binding protein MopA, 265 aa; fasta scores: opt: 280 z-score: 319.9 E(): 2.2e-10; 41.7% identity in 139 aa overlap 3 4 14 2 [Reporter]SCO5092 (20C17)_PCR/7342 [Gene]SCO5092/6688 NC_003888 SCBAC28G1.18, actVB, actinorhodin polyketide possible dimerase, len: 177 aa; identical to previously sequenced SW:DIM6_STRCO (EMBL:X63449) Streptomyces coelicolor actinorhodin polyketide dimerase ActI ORF6, ActVB, 177 aa Contains Pfam match to entry PF01613 Flavin_Reduct, Flavin reductase like domain 3 4 13 22 [Reporter]SCO1280 (1C13)_PCR/7340 [Gene]SCO1280/6687 NC_003888 2SCG18.27c, conserved hypothetical protein, len: 413 aa; similar to TR:Q9S203 (EMBL:AL109747) Streptomyces coelicolor hypothetical 48.6 kD protein SCJ21.04c, 448 aa; fasta scores: opt: 360 z-score: 403.3 E(): 5.6e-15; 30.7% identity in 391 aa overlap 3 4 13 20 [Reporter]SCO0409 (3K9)_PCR/7338 [Gene]SCO0409/6686 NC_003888 SCF51.08c, sapA spore-associated protein precursor, len: 154 aa; N-terminal region identical to SW:SAPA_STRCO (EMBL:M20145) Streptomyces coelicolor spore-associated protein precursor (fragment) SapA, 120 aa 3 4 13 19 [Reporter]SCO5544 (4G9)_PCR/7337 [Gene]SCO5544/6685 NC_003888 SC1C2.25c, cvnA1, unknown, len: 1329 aa; contains two possible transmembrane domains at around 100 and 400 aa, and probable coiled-coil domains from 112 to 144 and 360 to 398. Overall 35.6% identical to downstream gene SC1C2.22c, but with much higher similarity between aa 468 and 709 3 4 13 18 [Reporter]SCO6402 (5C9)_PCR/7336 [Gene]SCO6402/6684 NC_003888 SC3C8.20, unknown ORF in IS117, len: 117 aa; identical to YM1_STRCO mini-circle hypothetical 12.9 KD protein (117 aa). Note alternative ORF on opposite strand 3 4 13 17 [Reporter]SCO5815 (6O5)_PCR/7335 [Gene]SCO5815/6683 NC_003888 SC5B8.05, probable ATP-dependent DNA helicase, len:7 19 aa; similar in N-terminal half to many e.g. RECQ_ECOLIP1 5043 atp-dependent dna helicase recq (607 aa), fasta score s; opt: 546 z-score: 615.9 E(): 4.1e-27, 35.9% identity in 362 aa overlap. Contains PS00690 DEAH-box subfamily ATP-dep endent helicases signature and Pfam match to entry helicase _C PF00271, Helicases conserved C-terminal domain, score 91 .14 3 4 13 16 [Reporter]SCO5431 (7K5)_PCR/7334 [Gene]SCO5431/6682 NC_003888 SC6A11.07c, possible secreted nucleosidase, len: 287 aa; similar to many e.g. SW:P24247 (PFS_ECOLI) MTA/SAH nucleosidase (P46) from Escherichia coli (232 aa) fasta scores: opt: 211, z-score: 251.7, E(): 1.3e-06, 27.1% identity in 210 aa overlap. Contains Pfam match to entry PF01048 PNP_UDP_1, Phosphorylase family (which includes some nucleosidases) and possible N-terminal signal sequence 3 4 13 15 [Reporter]SCO4746 (8G5)_PCR/7333 [Gene]SCO4746/6681 NC_003888 SC6G4.24, possible lipase, len: 420 aa; some similar ity to e.g. TR:Q59695 (EMBL:L35343) Pseudomonas putida dihy drolipoamide acetyltransferase (370 aa), fasta scores; opt: 134 z-score: 204.4 E(): 0.00041, 27.0% identity in 352 aa overlap. Contains PS00120 Lipases, serine active site and P fam match to entry PF00561 abhydrolase, alpha/beta hydrolas e fold, score 79.50, E-value 7e-20. Contains possible hydrophobic membrane spanning region 3 4 13 14 [Reporter]SCO7722 (9C5)_PCR/7332 [Gene]SCO7722/6680 NC_003888 SC8D11.13, hypothetical protein, len: 194 aa; similar to TR:Q9L045 (EMBL:AL163672) Streptomyces coelicolor hypothetical 22.3 kDa protein SCE50.18c, 203 aa; fasta scores: opt: 588 z-score: 709.1 E(): 5.8e-32; 49.7% identity in 189 aa overlap 3 4 13 13 [Reporter]SCO6253 (10O1)_PCR/7331 [Gene]SCO6253/6679 NC_003888 SCAH10.18, possible two-component sensor kinase, len: 417 aa; similar to TR:O86631 EMBL; AL031155 Streptomyces coelicolor putative two-component sensor SC3A7.30, 429 aa; fasta scores: opt: 588 z-score: 615.4 E(): 6.6e-27; 35.0% identity in 412 aa overlap and C-terminus to C-terminus of TR:Q53893 (EMBL:U51332) Streptomyces coelicolor AbsA1, 571 aa; fasta scores: opt: 477 z-score: 492.4 E(): 4.7e-20; 40.1% identity in 262 aa overlap. Contains possible hydrophobic membrane spanning regions 3 4 13 12 [Reporter]SCO2347 (11K1)_PCR/7330 [Gene]SCO2347/6678 NC_003888 SCC8A.05c, possible integral membrane protein, len: aa; similar to others of undefined function eg. TR:P96229 (EMBL:Z83864) hypothetical 32.0 kD protein from Mycobacterium tuberculosis (302 aa) fasta scores; opt: 245, z-score: 295.9, E(): 5.1e-09, 32.8% identity in 195 aa overlap. Contains possible membrane-spanning hydrophobic regions and Pfam match to entry PF01169 UPF0016, Uncharacterized protein family UPF0016. 3 4 13 11 [Reporter]SCO4451 (12G1)_PCR/7329 [Gene]SCO4451/6677 NC_003888 SCD6.29, probable export protein, len: 541 aa; similar to TR:CAB61602 (EMBL:AL133210) Streptomyces coelicolor putative export protein, SCG11.21c, 533 aa; fasta scores: opt: 832 z-score: 842.6 E(): 0; 33.7% identity in 531 aa overlap and to SW:TCMA_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C resistance and export protein TcmA, 538 aa; fasta scores: opt: 701 z-score: 710.8 E(): 3.9e-32; 29.3% identity in 543 aa overlap. Contains possible hydrophobic membrane spanning regions 3 4 13 10 [Reporter]SCO2852 (13C1)_PCR/7328 [Gene]SCO2852/6676 NC_003888 SCE20.26c, possible membrane protein, len: 111 aa. Contains a possible N-terminal membrane spanning hydrophobic domain. 3 4 13 9 [Reporter]SCO0034 (13O21)_PCR/7327 [Gene]SCO0034/6675 NC_003888 SCJ4.15, unknown, len: 159 aa 3 4 13 8 [Reporter]SCO3361 (14K21)_PCR/7326 [Gene]SCO3361/6674 NC_003888 SCE94.12c, lrpA, probable AsnC-family transcriptional regulator, len: 150a a; previously sequenced therefore identical to TR:E1358530 (EMBL:AJ131213) LrpA, putative transcriptional regulator from Streptomyces coelicolor (150 aa) fasta scores; opt: 953, z-score: 1220.9, E(): 0, (100.0% identity in 150 aa overlap). Also similar to many amino acid responsive regulators e.g. TR:O33467 (EMBL:D89015) MdeR, transcriptional regulator from Pseudomonas putida (159 aa) fasta scores; opt: 380, z-score: 494.5, E(): 3.1e-20, (40.0% identity in 150 aa overlap). Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family. Also contains a possible helix-turn-helix at 19-40aa (+4.10 SD). 3 4 13 7 [Reporter]SCO4971 (15G21)_PCR/7325 [Gene]SCO4971/6673 NC_003888 2SCK31.31, possible dehydrogenase, len: 497 aa; similar to N-terminal domain of SW:XDH_MOUSE (EMBL:X75129) Mus musculus xanthine dehydrogenase/oxidase Xdh, 1335 aa; fasta scores: opt: 345 z-score: 361.7 E(): 1.3e-12 31.5% identity in 483 aa overlap and to TR:O54050 (EMBL:AJ001013) Rhodobacter capsulatus xanthine dehydrogenase (EC 1.1.1.204) XdhA, 462 aa; fasta scores: opt: 631 z-score: 669.1 E(): 9.8e-30; 38.2% identity in 495 aa overlap. Contains Pfam matches to entries PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains, PF01799 fer2_2, [2Fe-2S] binding domain and PF00941 dehydrog_molyb, Molybdopterin binding domain in dehydrogenase and match to Prosite entry PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature 3 4 13 6 [Reporter]SCO1175 (16C21)_PCR/7324 [Gene]SCO1175/6672 NC_003888 SCG11A.06c, hypothetical protein, len: 153 aa; similar to C-terminus of TR:O52556 (EMBL:AF040570) Amycolatopsis mediterranei esterase, 310 aa; fasta scores: opt: 303 z-score: 372.1 E(): 2.4e-13; 45.1% identity in 113 aa overlap 2 3 18 22 [Reporter]SCO1116 (1F18)_PCR/4931 [Gene]SCO1116/4499 NC_003888 2SCG38.09, hypothetical protein, len: 338 aa; similar to TR:P71751 (EMBL:Z81368) Mycobacterium tuberculosis hypothetical 29.7 kDa protein MTCY253.28c, 281 aa; fasta scores: opt: 210 z-score: 227.0 E(): 3.4e-05; 29.1% identity in 282 aa overlap 3 4 13 5 [Reporter]SCO0565 (17O17)_PCR/7323 [Gene]SCO0565/6671 NC_003888 SCF73.12c, probable polyprenyl synthetase, len: 352 aa; similar to members of the FPP/GGPP synthetases family e.g. GGPP_MYCTU probable geranylgeranyl pyrophosphate synthetase (359 aa), fasta scores; opt: 253 z-score: 299.8 E(): 2.5e-09 30.1% identity in 346 aa overlap, and ISPB_ECOLI octaprenyl-diphosphate synthase (323 aa), fasta scores; opt: 222 z-score: 264.3 E(): 2.4e-07, 30.0% identity in 337 aa overlap. Contains PS00444 Polyprenyl synthetases signature 2, and Pfam match to entry PF00348 polyprenyl_synt, Polyprenyl synthetases 2 3 18 21 [Reporter]SCO5774 (2B18)_PCR/4930 [Gene]SCO5774/4498 NC_003888 SC4H8.13c, gluD, probable glutamate permease, len: 299; similar to many members of the HisMQ subfamily of the binding-protein dependent transporters eg. GLUD_CORGL P48245 glutamate transport system permease protein from Corynebacterium glutamicum (273 aa), fasta scores; opt: 673 z-score: 843.4 E(): 0, 47.0% identity in 251 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature 3 4 13 4 [Reporter]SCO4893 (18K17)_PCR/7322 [Gene]SCO4893/6670 NC_003888 2SCK8.19c, possible transport integral membrane protein, len: 307 aa; similar to SW:MDCF_RHIME (EMBL:AF155772) Rhizobium meliloti putative malonate transporter MdcF, 320 aa; fasta scores: opt: 306 z-score: 339.0 E(): 2.6e-11; 27.4% identity in 299 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 18 20 [Reporter]SCO6809 (3N14)_PCR/4929 [Gene]SCO6809/4497 NC_003888 SC1A2.18c, possible integral membrane transport protein, len: 409 aa. Similar to several membrane transport proteins e.g. Listeria monocytogenes TR:Q9X769 (EMBL:AJ009627) multi drug resistance transporter (397 aa), fasta scores opt: 241 z-score: 264.5 E(): 2.5e-07 23.0% identity in 344 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a Prosite hit to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 2 3 18 19 [Reporter]SCO7207 (4J14)_PCR/4928 [Gene]SCO7207/4496 NC_003888 SC2H12.06, possible membrane protein, len: 286 aa. Contains possible membrane spanning hydrophobic domains. 2 3 18 18 [Reporter]SCO2380 (5F14)_PCR/4927 [Gene]SCO2380/4495 NC_003888 SC4A7.08, hypothetical protein, len: 273 aa; similar to TR:O53531 (EMBL:AL021925) Mycobacterium tuberculosis hypothetical 28.a kD protein MTV022.07c, 272 aa; fasta scores: opt: 1011 z-score: 1132.5 E(): 0; 56.8% identity in 271 aa overlap 2 3 18 17 [Reporter]SCO7759 (6B14)_PCR/4926 [Gene]SCO7759/4494 NC_003888 SC5E9.07, possible DNA-binding protein, len: 531aa; similar to many conserved hypothetical proteins eg. TR:Q9X8W1 (EMBL:AL078610) hypothetical protein from Streptomyces coelicolor (634 aa) fasta scores; opt: 1459, z-score: 1666.8, E(): 0, 48.8% identity in 531 aa overlap. Contains helix-turn-helix motif (Score 1136 (+3.06 SD)) at residue 18-39. Note possible alternative translational start sites. Also contains Pfam match to entry PF01590 GAF, GAF domain. 2 3 18 16 [Reporter]SCO6787 (7N10)_PCR/4925 [Gene]SCO6787/4493 NC_003888 SC6A5.36, acdH3, probable acyl-CoA dehydrogenase, len: 382 aa; similar to many (prokaryote and eukaryote) egs. TR:O33229 (EMBL:Z98209) probable acyl-CoA dehydrogenase from Mycobacterium tuberculosis (386 aa) fasta scores; opt: 1583, z-score: 1842.1, E(): 0, (62.7% identity in 378 aa overlap) and SW:ACDL_HUMAN acyl-CoA dehydrogenase from Homo sapiens (Human) (430 aa) fasta scores; opt: 1265, z-score: 1472.3, E(): 0, (51.8% identity in 380 aa overlap). Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase and Prosite matches to PS00072 Acyl-CoA dehydrogenases signature 1 and PS00073 Acyl-CoA dehydrogenases signature 2. 2 3 18 15 [Reporter]SCO6006 (8J10)_PCR/4924 [Gene]SCO6006/4492 NC_003888 SC7b7.03, probable integral membrane transport protein, len: 353 aa; similar to many members of the MALFG subfamily of the binding-protein-dependant transport system membrane proteins eg. MSMF_STRMU Q00750 multiple sugar-binding transport system permease protein (290 aa), fasta scores; opt: 393 z-score: 636.8 E(): 2.6e-28, 32.5% identity in 280 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature 2 3 18 14 [Reporter]SCO5507 (9F10)_PCR/4923 [Gene]SCO5507/4491 NC_003888 SC8D9.19, unknown, len: 69 aa 2 1 8 2 [Reporter]SCO7491 (20J12)_PCR/659 [Gene]SCO7491/599 NC_003888 SCBAC17A6.24, conserved hypothetical protein, len: 266aa; similar to many eg. SW:P76044 (YCJR_ECOLI) hypothetical protein from Escherichia coli (265 aa) fasta scores: opt: 571, Z-score: 667.8, 36.502% identity (37.209% ungapped) in 263 aa overlap 2 3 18 12 [Reporter]SCO4381 (11N6)_PCR/4921 [Gene]SCO4381/4490 NC_003888 SCD10.13, probable acetyl/propionyl CoA carboxylase, alpha subunit, len: 614 aa; similar to TR:CAB87214 (EMBL:AL163641) Streptomyces coelicolor acetyl/propionyl CoA carboxylase, alpha subunit AccC, 646 aa; fasta scores: opt: 1088 z-score: 1184.1 E(): 0; 46.6% identity in 644 aa overlap and to SW:BCCA_MYCTU (EMBL:Z19549) Mycobacterium tuberculosis acetyl/propionyl-coenzyme A carboxylase alpha chain AccA1, 654 aa ;fasta scores: opt: 939 z-score: 1022.0 E(): 0; 43.2% identity in 655 aa overlap. Contains 2x Pfam matches to entry PF00289 CPSase_L_chain, Carbamoyl-phosphate synthase (CPSase), Pfam match to entry PF00364 biotin_lipoyl, Biotin-requiring enzymes and matches to Prosite entries PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00188 Biotin-requiring enzymes attachment site 2 1 7 22 [Reporter]SCO1227 (1J8)_PCR/657 [Gene]SCO1227/598 NC_003888 2SCG1.02c, possible DNA-binding protein, len: 489 aa; similar to TR:CAB89011 (EMBL:AL353870) Streptomyces coelicolor hypothetical 50.9 kD protein SC6F7.03, 467 aa; fasta scores: opt: 177 z-score: 183.6 E(): 0.0092; 26.7% identity in 476 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and a possible helix-turn-helix motif at residues 44..65 (+3.67 SD). Also contains a TTA leucine codon (159), possible target for bldA regulation 2 1 7 21 [Reporter]SCO4426 (2F8)_PCR/656 [Gene]SCO4426/597 NC_003888 SCD6.04c, afsR, regulatory protein, len: 993 aa; identical to previously sequenced SW:AFSR_STRCO (EMBL:D90155) Streptomyces coelicolor regulatory protein AfsR, 993 aa. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 7 20 [Reporter]SCO2587 (3B8)_PCR/655 [Gene]SCO2587/596 NC_003888 SCC123.25c, proB, glutamate 5-kinase (EC 2.7.2.11), len: 383 aa. Highly similar to many other glutamate 5-kinases including: Escherichia coli SW:PROB_ECOLI(EMBL:X00786) (367 aa), fasta scores opt: 788 z-score: 846.3 E(): 0 38.1% identity in 367 aa overlap and Mycobacterium tuberculosis SW:PROB_MYCTU(EMBL:Z81451) (376 aa), fasta scores opt: 1193 z-score: 1276.9 E(): 0 55.9% identity in 367 aa overlap. Contains a Pfam matches to entries PF00696 aakinase, Aspartate kinases, Glutamate kinases and Gamma glutamate phospho-reductases and PF01472 PUA, PUA domain. 2 1 7 19 [Reporter]SCO6611 (4N4)_PCR/654 [Gene]SCO6611/595 NC_003888 SC1F2.08c, unknown, len: 186 aa 2 1 7 18 [Reporter]SCO5895 (5J4)_PCR/653 [Gene]SCO5895/594 NC_003888 SC3F7.15, redI, possible methyltransferase, len: 362 aa; weakly similar to several e.g. Streptomyces griseus TR:P72450 (EMBL:X95596) methyltransferase (242 aa), fasta scores; opt: 130 z-score: 204.9 E(): 0.00032, 27.6% identity in 192 aa overlap 2 1 7 17 [Reporter]SCO6647 (6F4)_PCR/652 [Gene]SCO6647/593 NC_003888 SC4G2.21c, possible integral membrane protein, len: 264 aa; similar to a hypothetical protein from Rhodobacter sphaeroides TR:O54078 (EMBL:AJ002398) (227 aa), fasta scores; opt: 493 z-score: 616.8 E(): 4.1e-27, 43.6% identity in 220 aa overlap. Also very weak similarity to PGSA_PSEFL (CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase) (195 aa) E:0.0018, 30.8% identity in 146 aa overlap. Contains PS00379 CDP-alcohol phosphatidyltransferases signature 2 1 7 16 [Reporter]SCO0319 (7B4)_PCR/651 [Gene]SCO0319/592 NC_003888 SC5G9.28c, unknown, len: 169 aa; probable CDS suggested by GC frameplot, positional base preference and amino acid composition 2 1 7 15 [Reporter]SCO2137 (7N24)_PCR/650 [Gene]SCO2137/591 NC_003888 SC6G10.10c, unknown, len: 447aa; some similarity to TR:Q60218 (EMBL:L10330) TraN from plasmid RP4 of Escherichia coli (261 aa) fasta scores; opt: 176, z-score: 185.4, E(): 0.005, (29.5% identity in 224 aa overlap). Contains probable coiled coil region. 2 1 7 14 [Reporter]SCO6587 (8J24)_PCR/649 [Gene]SCO6587/590 NC_003888 SC8A6.08, probable dehydrogenase, len: 520 aa; some similarity to many diverse dehydrogenases, e.g. SSDH_RAT su ccinate semialdehyde dehydrogenase (488 aa), fasta scores; opt: 308 z-score: 301.8 E(): 1.4e-09, 26.0% identity in 439 aa overlap 3 4 13 2 [Reporter]SCO5969 (20O13)_PCR/7320 [Gene]SCO5969/6669 NC_003888 SCBAC16H6.04, conserved hypothetical protein, len: 402aa: similar to many eg. TR:Q9K5U1 (EMBL:AP001520) BH3997 protein from Bacillus halodurans (101 aa) fasta scores; opt: 282, Z-score: 358.7, 40.196% identity (42.268% ungapped) in 102 aa overlap. 3 4 12 22 [Reporter]SCO1250 (1O9)_PCR/7318 [Gene]SCO1250/6668 NC_003888 2SCG1.25, possible acetyltransferase, len: 141 aa; similar to TR:CAB72694 (EMBL:AL139074) Campylobacter jejuni putative acetyltransferase CJ0225, 148 aa; fasta scores: opt: 187 z-score: 246.3 E(): 3e-06; 26.5% identity in 132 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 3 4 12 21 [Reporter]SCO3471 (2K9)_PCR/7317 [Gene]SCO3471/6667 NC_003888 SCE65.07c, dagA, extracellular agarase precursor, len: 309 aa; identical to previously sequenced SW:AGAR_STRCO (EMBL:X05811) Streptomyces coelicolor extracellular agarase precursor (EC 3.2.1.81) DagA, 309 aa and similar to TR:Q59078 (EMBL:M73783) Alteromonas atlantica (Pseudomonas atlantica) beta-agarase I precursor (EC 3.2.1.81), 290 aa; fasta scores: opt: 385 z-score: 446.1 E(): 1.8e-17; 33.2% identity in 304 aa overlap. Contains match to Prosite entry PS01034 Glycosyl hydrolases family 16 active sites. Contains N-terminal region signal sequence peptide 3 4 12 20 [Reporter]SCO4071 (3G9)_PCR/7316 [Gene]SCO4071/6666 NC_003888 SCD25.07, purC, phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6), len: 299 aa. Highly similar to many e.g. Mycobacterium tuberculosis SW:PUR7_MYCTU (EMBL; Z80226) phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6) (saicar synthetase) PurC or MTCY369.24 (297 aa), fasta scores: opt: 1085 z-score: 1276.6 E(): 0 58.3% identity in 278 aa overlap. Contains a PS01057 SAICAR synthetase signature 1, PS01058 SAICAR synthetase signature and a Pfam match to entry PF01259 SAICAR_synt, SAICAR synthetase. 3 4 12 19 [Reporter]SCO5536 (4C9)_PCR/7315 [Gene]SCO5536/6665 NC_003888 SC1C2.17, unknown, len: 65 aa 3 4 12 18 [Reporter]SCO6578 (5O5)_PCR/7314 [Gene]SCO6578/6664 NC_003888 SC3F9.13, unknown, len: 391 aa; contains PS00730 AP endonucleases family 2 signature 2 3 4 12 17 [Reporter]SCO6681 (6K5)_PCR/7313 [Gene]SCO6681/6663 NC_003888 SC5A7.31, unknown, len 930 aa; some similarity to several e.g. PKN2_MYXXA serine-threonine-protein kinase Pkn2 (Myxococcus xanthus) (830 aa), Smith-Waterman scores; E: 1.5e-06, 23% identity in 829 bp overlap. Also has short segment of similarity to the active site regions of several Serine-Threonine protein kinases e.g. PKAA_STRCO P54739 serine-threonine protein kinase pkaA (533 aa), blastp scores; Expect= 3.7e-05, 39% identity in 27 aa overlap. Contains possible transmembrane anchor and six degenerate repeats of the sequence VDETTR 3 4 12 16 [Reporter]SCO7280 (7G5)_PCR/7312 [Gene]SCO7280/6662 NC_003888 SC5H1.12, clpP4, probable ATP-dependent Clp protease proteolytic subunit 2, len: 200 aa; similar to many e.g. clpP2 (EMBL:AF071885) Streptomyces coelicolor ATP-dependent Clp protease proteolytic subunit 2 (236 aa), fasta scores; opt: 716 z-score: 853.7 E(): 0, 56.0% identity in 193 aa overlap. Also similar to adjacent CDS, SC5H1.11, probable ATP-dependent Clp protease proteolytic subunit 1 (217 aa) (39.3% identity in 201 aa overlap). Contains Pfam match to entry PF00574 CLP_protease, Clp protease, score 268.60, E-value 8.1e-77 2 3 18 11 [Reporter]SCO3952 (12J6)_PCR/4920 [Gene]SCO3952/4489 NC_003888 SCD78.19c, unknown, len: 152 aa 3 4 12 15 [Reporter]SCO4739 (8C5)_PCR/7311 [Gene]SCO4739/6661 NC_003888 SC6G4.17, probable lipoprotein, len: 268 aa; contain s N-terminal signal sequence and appropriately positioned P S00013 Prokaryotic membrane lipoprotein lipid attachment si te 2 3 18 10 [Reporter]SCO3075 (13F6)_PCR/4919 [Gene]SCO3075/4488 NC_003888 SCE25.16c, possible transcriptional regulator, len: 289 aa; similar to SW:HEXR_PSEAE (EMBL:AF029673) Pseudomonas aeruginosa hex regulon repressor HexR, 285 aa; fasta scores: opt: 197 z-score: 227.5 E(): 3.2e-05; 27.7% identity in 220 aa overlap. Contains possible helix-turn-helix motif at residues 50..71 (+2.54 SD) 3 4 12 14 [Reporter]SCO2646 (9O1)_PCR/7310 [Gene]SCO2646/6660 NC_003888 SC8E4A.16c, possible integral membrane protein, len: 320 aa; similar to SW:PECM_ERWCH (EMBL:X74409) Erwinia chrysanthemi PecM protein, 297 aa; fasta scores: opt: 781 z-score: 802.2 E(): 0; 43.9% identity in 278 aa overlap and to TR:AAF10091 (EMBL:AE001910) Deinococcus radiodurans conserved hypothetical protein DR0512, 301 aa; fasta scores: opt: 310 z-score: 324.9 E(): 1.1e-10; 29.0% identity in 286 aa overlap; fasta scores: opt: 748 z-score: 768.7 E(): 0; 46.5% identity in 284 aa overlap. Contains tow matches to Pfam entry PF00892 DUF6, Integral membrane protein and various possible hydrophobic membrane spanning regions 2 3 18 9 [Reporter]SCO0676 (14B6)_PCR/4918 [Gene]SCO0676/4487 NC_003888 SCF91.36, possible integral membrane sensor protein, len: 864 aa. Similar in the N-terminus to several sensory transduction histidine kinases e.g. Escherichia coli SW:DCUS_ECOLI(EMBL:U14003) sensor protein DcuS (EC 2.7.3.-) (543 aa), fasta scores opt: 499 z-score: 546.9 E(): 4.4e-23 30.8% identity in 331 aa overlap. Highly similar in the C-terminus to several proteins from Streptomyces coelicolor including: TR:CAB41270(EMBL:AL049707) conserved hypothetical protein (fragment) ( >436 aa), fasta scores opt: 1207 z-score: 1324.9 E():0 48.0% identity in 423 aa overlap and TR:CAB44394(EMBL:AL078610) putative regulatory protein SCH35.33C (422 aa), fasta scores opt: 309 z-score: 340.2 E(): 1.4e-11 32.0% identity in 284 aa overlap. Contains a Pfam match to entry PF01590 GAF, GAF domain. Also contains a possible N-terminal signal sequence and multiple possible membrane spanning hydrophobic domains. 2 3 18 8 [Reporter]SCO3881 (15N2)_PCR/4917 [Gene]SCO3881/4486 NC_003888 StH24.03, rnpA, probable ribonuclease P component, len: 123 aa; previously sequenced as SW:RNPA_STRCO (EMBL:M82836), RnpA. Similar to SW:RNPA_STRBI (EMBL:M83112), rnpA, Streptomyces bikiniensis ribonuclease P protein component (123 aa), fasta scores; opt: 704 z-score: 879.0 E(): 0, 88.6% identity in 123 aa overlap .Contains Pfam match to entry PF00825 Ribonuclease_P, Ribonuclease P, score 179.50, E-value 5.3e-50 and PS00648 Bacterial Ribonuclease P protein component signature 2 3 18 7 [Reporter]SCO2990 (16J2)_PCR/4916 [Gene]SCO2990/4485 NC_003888 SCE50.18c, hypothetical protein, len: 203 aa; similar to TR:Q9Z552 (EMBL:AL03521) Streptomyces coelicolor hypothetical 17.8 kD protein, 162 aa; fasta scores: opt: 203 z-score: 257.9 E(): 6.4e-07; 29.7% identity in 165 aa overlap and to TR:Q9AK06 (EMBL:AL512944) Streptomyces coelicolor hypothetical protein SC8D11.13, 194 aa; fasta scores: opt: 588 Z-score: 704.3 E(): 1.4e-31; 49.735% identity in 189 aa overlap 2 3 18 6 [Reporter]SCO3172 (17F2)_PCR/4915 [Gene]SCO3172/4484 NC_003888 SCE87.23c, probable monooxygenase, len: 519 aa. Highly similar to many including: Acinetobacter sp SW:CYMO_ACISP (EMBL:M19029) cyclohexanone monooxygenase (EC 1.14.13.22) (542 aa), fasta scores opt: 459 z-score: 510.7 E(): 4.5e-21 28.0% identity in 511 aa overlap and Mycobacterium tuberculosis TR:O53294(EMBL:AL021287) monooxygenase (524 aa), fasta scores opt: 1522 z-score: 1690.4 E():0 47.4% identity in 485 aa overlap. Contains a Pfam match to entry PF00743 FMO-like, Flavin-binding monooxygenase-like. 2 3 18 5 [Reporter]SCO1469 (18B2)_PCR/4914 [Gene]SCO1469/4483 NC_003888 SCL6.26c, unknown, len: 701 aa. C-terminal region rich in Glycine and Alanine residues. Contains Pfam match to entry PF00089 trypsin, Trypsin 2 3 18 4 [Reporter]SCP1.127 (18N22)_PCR/4913 [Gene]SCP1.127/4482 NC_003888 SCP1.127, unknown, len: 259aa; 2 3 18 3 [Reporter]SCP1.14c (19J22)_PCR/4912 [Gene]SCP1.14c/4481 NC_003888 SCP1.14c, unknown, len: 382aa; 2 1 7 13 [Reporter]SCO5834 (9F24)_PCR/648 [Gene]SCO5834/589 NC_003888 SC9B10.01c, putative membrane protein, len: 248 aa. Contains possible hydrophobic membrane spanning region 2 3 18 2 [Reporter]SCO3968 (20B22)_PCR/4911 [Gene]SCO3968/4480 NC_003888 SCBAC25E3.05c, possible integral membrane protein, len: 284aa: similar to many from Streptomyces coelicolor eg. TR:Q9RCU (EMBL:AL136149) putative integral membrane protein SCM2.18c (256 aa) fasta scores; opt: 254, Z-score: 265.5, 37.631% identity (47.162% ungapped) in 287 aa overlap. Contains multiple possible membrane-spanning hydrophobic regions. 2 1 7 12 [Reporter]SCO2730 (10B24)_PCR/647 [Gene]SCO2730/588 NC_003888 SCC57A.01, possible regulator, partial CDS, len: > 47 aa. Similar to the C-terminus of Streptomyces coelicolor TR:CAB61163 (EMBL:AL132973) putative TetR-family transcriptional regulator (194 aa), fasta scores opt: 85 z-score: 133.1 E():5 58.1% identity in 31 aa overlap.,SCC46.15, possible regulator (fragment), len: >89 aa; N-terminal region of TR:CAB66269 (EMBL:AL136519) Streptomyces coelicolor putative regulator (fragment) SCC57A.01. 2 1 7 11 [Reporter]SCO4663 (11N20)_PCR/646 [Gene]SCO4663/587 NC_003888 SCD40A.09c, unknown, len: 117 aa 2 1 7 10 [Reporter]SCO2935 (12J20)_PCR/645 [Gene]SCO2935/586 NC_003888 SCE19A.35c, probable transcriptional regulator, len: 220 aa; similar to many e.g. SW:ICLR_ECOLI (EMBL:M31761), IclR, Escherichia coli acetate operon repressor (274 aa), fasta scores; opt: 206 z-score: 234.8 E(): 9.6e-06, 29.2% identity in 154 aa overlap. Similar to TR:O05148 (EMBL:U89712), hppR, Rhodococcus globerulus putative regulator of 3-(3-hydroxyphenyl)propionic acid catabolic pathway (206 aa) (44.8% identity in 174 aa overlap). Contains probable helix-turn-helix motif at aa 27-48 (Score 1663, +4.85 SD) 2 1 7 9 [Reporter]SCO1824 (13F20)_PCR/644 [Gene]SCO1824/585 NC_003888 SCI8.09, ssp, secreted subtilisin-like protease, len: 512 aa. Almost identical to the Streptomyces lividans TR:Q54400 (EMBL: L41655) subtilisin-like protease precursor (512 aa), fasta scores opt: 3375 z-score: 3428.7 E(): 0 99.6% identity in 512 aa overlap. Contains a Pfam match to entry PF00082 Peptidase_S8, Subtilase family and Prosite hits to PS00137 Serine proteases, subtilase family, histidine active site, PS00138 Serine proteases, subtilase family, serine active site and PS00136 Serine proteases, subtilase family, aspartic acid active site. Also contains a possible N-terminal signal sequence. 2 1 7 8 [Reporter]SCO0095 (14B20)_PCR/643 [Gene]SCO0095/584 NC_003888 SCJ11.24c, hypothetical protein, part of noncomposite transposon, len: 239 aa; weakly similar to SW:YAFD_ECOLI hypothetical protein from Escherichia coli (259 aa) fasta scores; opt: 112, z-score: 130.4, E(): 6.7, (25.0% identity in 208 aa overlap). 2 1 7 7 [Reporter]SCO3544 (15N16)_PCR/642 [Gene]SCO3544/583 NC_003888 SCH5.07c, putative membrane protein, len: 132 aa. Contains possible hydrophobic membrane spanning regions 2 1 7 6 [Reporter]SCO3680 (16J16)_PCR/641 [Gene]SCO3680/582 NC_003888 SCH35.44, unknown, len: 97aa; 2 1 7 5 [Reporter]SCO1779 (17F16)_PCR/640 [Gene]SCO1779/581 NC_003888 SCI51.19c, hypothetical protein, len: 401 aa; unknown function, C-terminus shows weak similarity to SW:YPJH_BACSU (EMBL:L47709), YpjH, Bacillus subtilis hypothetical protein (377 aa), fasta scores; opt: 211 z-score: 223.0 E(): 1e-05, 28.0% identity in 193 aa overlap. Shows similarity to TR:Q9Z5B7 (EMBL:AL035478) S.coelicolor possible transferase (402 aa) (31.9% identity in 407 aa overlap). Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1 2 1 7 3 [Reporter]SCO5333 (19N12)_PCR/638 [Gene]SCO5333/580 NC_003888 SCBAC5H2.02c, conserved hypothetical protein, len: 301 aa; similar to TR:Q9RU61 (EMBL:AE001997) Deinococcus radiodurans conserved hypothetical protein DR1533, 299 aa; fasta scores: opt: 496 z-score: 583.0 E(): 6.7e-25; 46.3% identity in 298 aa overlap. Contains Pfam match to entry PF02182 G9a, Domain of unknown function 3 4 12 13 [Reporter]SCO6249 (10K1)_PCR/7309 [Gene]SCO6249/6659 NC_003888 SCAH10.14c, probable 3-oxoacyl-[acyl-carrier-protein] reductase, len: 258 aa; similar to TR:CAB42951 (EMBL:AL049863) Streptomyces coelicolor putative dehydrogenase/reductase, SC5H1.26, 245 aa; fasta scores: opt: 560 z-score: 573.5 E(): 1.4e-24; 45.1% identity in 246 aa overlap and to SW:FABG_BACSU (EMBL:U59433) Bacillus subtilis, 3-oxoacyl-[acyl-carrier-protein] reductase, FabG 246 aa; fasta scores: opt: 471 z-score: 484.6 E(): 1.3e-19; 36.0% identity in 247 aa overlap 3 4 12 12 [Reporter]SCO2708 (11G1)_PCR/7308 [Gene]SCO2708/6658 NC_003888 SCC61A.29, possible integral membrane protein, len: 438aa; weakly similar to TR:Q51524 (EMBL:X83916) PilO, required for glycosylation of Pseudomonas aeruginosa 1244 pilin (461 aa) fasta scores; opt: 165, z-score: 184.2, E(): 0.0085, 25.7% identity in 439 aa overlap. 3 4 12 11 [Reporter]SCO4447 (12C1)_PCR/7307 [Gene]SCO4447/6657 NC_003888 SCD6.25, possible integral membrane protein, len: 362 aa; similar to TR:CAB56388 (EMBL:AL118515) Streptomyces coelicolor putative integral membrane protein SCD17.11, 454 aa; fasta scores: opt: 715 z-score: 760.4 E(): 0; 41.6% identity in 368 aa overlap. Contains possible hydrophobic membrane spanning regions 3 4 12 10 [Reporter]SCO3532 (12O21)_PCR/7306 [Gene]SCO3532/6656 NC_003888 SCE2.13, unknown, len: 158aa; 3 4 12 9 [Reporter]SCO2633 (13K21)_PCR/7305 [Gene]SCO2633/6655 NC_003888 SC8E4A.03, sodF, superoxide dismutase [Fe-Zn] (EC 1.15.1.1), len: 213 aa; identical to previously sequenced SW:SODF_STRCO (EMBL:AF012087) Streptomyces coelicolor superoxide dismutase [Fe-Zn] (EC 1.15.1.1) SodF and highly similar to SW:SODM_PROFR (PIR:S41106) Propionibacterium freudenreichii shermanii superoxide dismutase [Mn-Fe] (EC 1.15.1.1) SodA, 201 aa; fasta scores: opt: 792 z-score: 939.3 E(): 0; 57.9% identity in 202 aa overlap. Contains Pfam match to entry PF00081 sodfe, Iron/manganese superoxide dismutases (SODM) and match to Prosite entry PS00088 Manganese and iron superoxide dismutases signature 3 4 12 8 [Reporter]SCO6586 (14G21)_PCR/7304 [Gene]SCO6586/6654 NC_003888 SC8A6.07, sucD1, probable succinyl-coa synthetase alpha chain, len: 308 aa; highly similar to many e.g. SUCD_MYC TU succinyl-coa synthetase alpha chain (EC 6.2.1.5) (303 aa ), fasta scores; opt: 1273 z-score: 1425.5 E(): 0, 64.7% identity in 300 aa overlap. Contains PS01216 ATP-citrate lyas e / succinyl-CoA ligases family signature 1, PS00399 ATP-ci trate lyase / succinyl-CoA ligases family active site and P fam match to entry PF00549 ligase-CoA, CoA-ligases, score 1 32.10, E-value 9.3e-36 3 4 12 7 [Reporter]SCO1448 (15C21)_PCR/7303 [Gene]SCO1448/6653 NC_003888 SCL6.05, probable transport protein, len: 403 aa; similar to TR:AAF12043 (EMBL:AE002079) Deinococcus radiodurans transport protein, putative DR2502, 471 aa; fasta scores: opt: 687 z-score: 761.8 E(): 0; 39.0% identity in 359 aa overlap and to SW:MOSC_RHIME (EMBL:L17071) Rhizobium meliloti membrane protein MosC, 407 aa; fasta scores: opt: 496 z-score: 552.3 E(): 2.4e-23; 31.0% identity in 378 aa overlap. Contains match to Prosite entry PS00217 Sugar transport proteins signature 2. Contains also possible hydrophobic membrane spanning regions 3 4 12 6 [Reporter]SCO0330 (16O17)_PCR/7302 [Gene]SCO0330/6652 NC_003888 SCF12.09, probable short chain oxidoreductase, len: 249 aa; similar to many eg. TR:O86853 (EMBL:AJ007731) 3-ketoacyl-ACP/CoA reductase from Streptomyces coelicolor (244 aa) fasta scores; opt: 515, z-score: 576.2, E(): 9.6e-25, (40.2% identity in 244 aa overlap and to SW:FABG_BACSU (EMBL:U59433) Bacillus subtilis 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) FabG, 246 aa; fasta scores: opt: 473 Z-score: 524.5 E(): 1.4e-21; 37.500% identity in 248 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase; Pfam match to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and two Prosite matches to PS00061 Short-chain dehydrogenases/reductases family signature 2 3 17 22 [Reporter]SCO1111 (1B18)_PCR/4909 [Gene]SCO1111/4479 NC_003888 2SCG38.04c, conserved hypothetical protein, len: 268 aa; similar to TR:O53782 (EMBL:AL021943) Mycobacterium tuberculosis hypothetical 26.5 kDa protein MTV040.02, 240 aa; fasta scores: opt: 735 z-score: 848.5 E(): 0; 51.4% identity in 218 aa overlap 3 4 12 5 [Reporter]SCO1160 (17K17)_PCR/7301 [Gene]SCO1160/6651 NC_003888 SCG8A.14c, cvnA3, possible membrane protein, len: 862 aa; similar to many from Streptomyces coelicolor e.g. TR:Q9X836 (EMBL:AL049727) putative large secreted protein (877 aa) fasta scores; opt: 1248, z-score: 1146.3, E(): 0, 34.7% identity in 836 aa overlap. Contains possible membrane-spanning hydrophobic regions. 2 3 17 21 [Reporter]SCO5636 (2N14)_PCR/4908 [Gene]SCO5636/4478 NC_003888 SC6A9.31, korSA, transcriptional regulator, len: 259 aa; almost identical to TR:E1286465 (EMBL:AJ005260) KorSA from Streptomyces ambofaciens plasmid pSAM2 (259 aa); 98.5% identity in 259 aa overlap and similar to many members of the gntR family e.g. KORA_STRLI KorA protein (241 aa), fasta scores; opt: 187 z-score: 247.3 E(): 1.6e-06, 26.7% identity in 240 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family, score 61.80, E-value 1.6e-17 3 4 12 4 [Reporter]SCO0355 (18G17)_PCR/7300 [Gene]SCO0355/6650 NC_003888 SCF41.14, unknown, len: 113 aa. Shares a low level of similarity with Bacillus subtilis TR:O05263 (EMBL; Z93938) hypothetical 12.6 KD protein (104 aa), fasta scores opt: 129 z-score: 179.4 E(): 0.012 31.3% identity in 96 aa overlap. 2 3 17 20 [Reporter]SCO6805 (3J14)_PCR/4907 [Gene]SCO6805/4477 NC_003888 SC1A2.14, possible integral membrane efflux protein, len: 434 aa. Similar to several membrane efflux proteins including: Mycobacterium smegmatis TR:O31137(EMBL:AF030344) Tetracycline-resistance determinant, TetV (419 aa), fasta scores opt: 521 z-score: 542.1 E(): 8.7e-23 28.6% identity in 406 aa overlap and Streptomyces coelicolor TR:CAB51438(EMBL:AL096884) putative integral membrane efflux protein, SC4G6.15C (384 aa), fasta scores opt: 403 z-score: 421.7 E(): 4.4e-16 30.1% identity in 389 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 2 3 17 19 [Reporter]SCO5486 (4F14)_PCR/4906 [Gene]SCO5486/4476 NC_003888 SC2A11.20, probable pyridoxal-phosphate-dependent aminotransferase, len: 389 aa; similar to many e.g. NIFS_ANASP Anaebaena NifS protein (400 aa), fasta scores; opt: 855 z-score: 1070.8 E(): 0, 40.4% identity in 384 aa overlap and SPL1_CANAL Candida albicans tRNA splicing protein SPL1 (488 aa), fasta scores; opt: 791 z-score: 912.8 E(): 0, 36.6% identity in 388 aa overlap. Contains Pfam match to entry PF00266 aminotran_5, Aminotransferases class-V, score 259.00, E-value 6.2e-74 2 3 17 18 [Reporter]SCO2376 (5B14)_PCR/4905 [Gene]SCO2376/4475 NC_003888 SC4A7.04, hypothetical protein, len: 295 aa; similar to TR:Q9L067 (EMBL:AL163641) Streptomyces coelicolor hypothetical protein SCC105.18, 298 aa; fasta scores: opt: 556 Z-score: 591.0 E(): 2.8e-25; 37.415% identity in 294 aa overlap 2 3 17 17 [Reporter]SCO6523 (6N10)_PCR/4904 [Gene]SCO6523/4474 NC_003888 SC5C7.08, unknown, len: 346 aa; similar to hypothetical proteins from M. tuberculosis YZ34_MYCTU MTCY31.34 (372 aa), fasta scores; opt: 678 z-score: 492.0 E(): 3.7e-20, 42.3% identity in 298 aa overlap, and to Saccharomyces cerevisiae TR:Q02883 (EMBL:U43281) LPG6P (468 aa), fasta scores; opt: 491 z-score: 495.9 E(): 2.2e-20, 35.5% identity in 262 aa overlap 2 3 17 16 [Reporter]SCO6783 (7J10)_PCR/4903 [Gene]SCO6783/4473 NC_003888 SC6A5.32c, unknown, len: 155 aa; similar to TR:Q55966 (EMBL:D64005) hypothetical protein from Synechocystis sp. PCC6803 (180 aa) fasta scores; opt: 161, z-score: 214.8, E(): 0.00012, (28.3% identity in 180 aa overlap). 2 2 1 8 [Reporter]SCO3767 (14J11)_PCR/2527 [Gene]SCO3767/2299 NC_003888 SCH63.14, hypothetical protein, len: 151 aa; similar to plasmid encoded TR:Q52355 (EMBL:L38824) Serratia marcescens tellurite resistance protein TerB, plasmid R478, 151 aa; fasta scores: opt: 257 z-score: 320.7 E(): 2.4e-10; 26.5% identity in 151 aa overlap and to TR:CAC44659 (EMBL:AL596248) Streptomyces coelicolor conserved hypothetical protein SCBAC17A6.14c, 191 aa; fasta scores: opt: 588 Z-score: 695.8 E(): 4e-31; 65.185% identity in 135 aa overlap 2 3 17 15 [Reporter]SCO5308 (8F10)_PCR/4902 [Gene]SCO5308/4472 NC_003888 SC6G9.25, conserved hypothetical protein, len: 293 aa; unknown function, similar to the N-terminal ends of hypothetical proteins from Mycobacterium tuberculosis e.g. TR:P95226 (EMBL:Z86089) Mycobacterium tuberculosis hypothetical protein (397 aa), fasta scores; opt: 534 z-score: 643.4 E(): 1.7e-28, 36.6% identity in 292 aa overlap and to the C-terminal end of TR:O34398 (EMBL:Z99110) Bacillus subtilis hypothetical protein (611 aa) (29.8% identity in 282 aa overlap). Also similar to S. coelicolor hypothetical protein SC4C6.19 (341 aa) (34.6% identity in 295 aa overlap) and (EMBL:AL033505), SC1E6.07, S.coelicolor hypothetical protein (319 aa) (32.8% identity in 259 aa overlap). Alternative start codons are present at codons 4, 7, 12 and 20 2 2 1 7 [Reporter]SCO3782 (15F11)_PCR/2526 [Gene]SCO3782/2298 NC_003888 SCH63.29c, possible regulatory protein, len: 449 aa; similar to TR:O86603 (EMBL:AL031155) Streptomyces coelicolor transcriptional regulator SC3A7.02c, 892 aa; blastp scores: Score = 148 (52.1 bits), Expect = 6.7e-07, P = 6.7e-07; Identities = 98/317 (30%), Positives = 130/317 (41%). Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and 4x matches to entry PF00515 TPR, TPR Domain 2 3 17 14 [Reporter]SCO5502 (9B10)_PCR/4901 [Gene]SCO5502/4471 NC_003888 SC8D9.14, possible integral membrane export protein, len: 748aa; similar to many eg. TR:Q53902 (EMBL:M64683) an intergral membrane protein involved in the export of actinorhodin from Streptomyces coelicolor (711 aa) fasta scores; opt: 353, z-score: 385.2, E(): 3.8e-14, (28.2% identity in 731 aa overlap). 2 1 7 2 [Reporter]SCO1299 (20F12)_PCR/637 [Gene]SCO1299/579 NC_003888 SCBAC36F5.10, probable transcriptional regulatory protein, len: 148 aa; similar to SW:LRPC_BACSU (EMBL:AB001488) Bacillus subtilis transcriptional regulator LrpC, 144 aa; fasta scores: opt: 294 Z-score: 365.8 bits: 73.6 E(): 9.1e-13; 34.848% identity in 132 aa overlap. Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family and possible helix-turn-helix motif at residues 23..44 (+3.08 SD) 2 2 1 6 [Reporter]SCO0866 (16B11)_PCR/2525 [Gene]SCO0866/2297 NC_003888 SCM2.19c, probable ECF-family sigma factor, len: 213 aa. Highly similar to many sigma factors including: Streptomyces coelicolor TR:O86856 (EMBL:AJ007313) sigma factor SigT (236 aa), fasta scores opt: 292 z-score: 354.1 E(): 2.4e-12 35.7% identity in 185 aa overlap and Mycobacterium tuberculosis SW:RPSC_MYCTU( EMBL:Z73966) probable RNA polymerase sigma-C factor SigC (185 aa), fasta scores opt: 530 z-score: 636.2 E(): 4.6e-28 51.7% identity in 178 aa overlap. Note this CDS appears to be a duplicated on this cosmid and shares 68.5 % identity, in 197 aa overlap, with the upstream gene, SCM2.17c. Note that the predicted product of SCM2.19c is 17 aa longer than that of SCM2.17c. Contains a Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) and a possible helix-turn-helix motif situated between residues 169..190. 2 3 17 13 [Reporter]SCO2501 (10N6)_PCR/4900 [Gene]SCO2501/4470 NC_003888 SCC121.04c, possible DNA-binding protein, len: 298 aa; similar to TR:CAB52924 (EMBL:AL109949) Streptomyces coelicolor putative DNA-binding protein SCJ11.39c, 279 aa; fasta scores: opt: 713 z-score: 847.3 E(): 0; 44.0% identity in 266 aa overlap and to TR:CAB53273 (EMBL:AL109972) Streptomyces coelicolor putative DNA-binding protein SCJ9A.12c, 279 aa; fasta scores: opt: 416 z-score: 497.4 E(): 2.6e-20; 35.1% identity in 276 aa overlap 2 1 6 22 [Reporter]SCO5255 (1F8)_PCR/635 [Gene]SCO5255/578 NC_003888 2SC7G11.17, signal peptidase protein, len: 146 aa; previously sequenced as TR:O51920 (EMBL:AF104994) Streptomyces coelicolor signal peptidase-like protein, 148 aa. Contains Pfam match to entry PF00461 Peptidase_S26, Signal peptidase I and match to Prosite entry PS00501 Signal peptidases I serine active site 2 2 1 5 [Reporter]SCO5026 (17N7)_PCR/2524 [Gene]SCO5026/2296 NC_003888 SCK15.28, hypothetical protein, len: 272 aa; similar to TR:O06593 (EMBL:Z95586) Mycobacterium tuberculosis hypothetical 27.9 kD protein MTCY336.07c, 252 aa; fasta scores: opt: 968 z-score: 1156.3 E(): 0; 57.5% identity in 254 aa overlap 2 1 6 21 [Reporter]SCO4644 (2B8)_PCR/634 [Gene]SCO4644/577 NC_003888 SCD82.15c, add, adenosine deaminase, len: 343 aa; highly similar to SW:ADD_STRVG (EMBL:D50624) Streptomyces virginiae adenosine deaminase (EC 3.5.4.4), Add, 339 aa; fasta scores: opt: 1989 z-score: 2275.3 E(): 0; 85.6% identity in 341 aa overlap. Contains Pfam match to entry PF00962 A_deaminase, Adenosine/AMP deaminase and match to Prosite entry PS00485 Adenosine and AMP deaminase signature 2 2 1 4 [Reporter]SCO3654 (18J7)_PCR/2523 [Gene]SCO3654/2295 NC_003888 SCH10.32c, probable two-component system sensor kinase, len: 441 aa; similar to many e.g. TR:Q53893 (EMBL:U51332), AbsA1, Streptomyces coelicolor histidine kinase homolog involved in negative regulation of antibiotic synthesis (571 aa), fasta scores; opt: 474 z-score: 452.1 E(): 7.2e-18, 32.8% identity in 381 aa overlap. Also similar to SW:NARX_ECOLI (EMBL:X13360), NarX, NarR, Escherichia coli nitrate/nitrite sensor protein (598 aa) (28.2% identity in 234 aa overlap). Contains hydrophobic, probable membrane-spanning regions. Similar to many other putative two-component system sensor kinases from Streptomyces coelicolor e.g. SCH10.17c, probable two-component system sensor (472 aa) (38.0% identity in 389 aa overlap). The start codon may be further downstream, which would agree with some other bacterial two-component system sensors; however, NarX has a length comparable to this prediction 2 1 6 20 [Reporter]SCO1598 (3N4)_PCR/633 [Gene]SCO1598/576 NC_003888 SCI35.20c, rplT, 50S ribosomal protein L20, len: 127 aa; highly similar to many e.g. RL20_ECOLI 50S ribosomal protein L20 (117 aa), fasta scores; opt: 431 z-score: 854.1 E(): 0, 60.3% identity in 116 aa overlap. Contains Pfam match to entry PF00453 L20, Ribosomal protein L20, score 174.80, E-value 1.6e-54 2 2 1 3 [Reporter]SCP1.151c (19F7)_PCR/2522 [Gene]SCP1.151c/2294 NC_003888 SCP1.151c, possible sigma factor, len: 174aa; weakly similar to many eg. TR:Q9X8Z1 (EMBL:AL078610) putative ECF sigma factor from Streptomyces coelicolor (184 aa) fasta scores; .opt: 187, z-score: 235.0, E(): 1.3e-05, 28.0% identity in 164 aa overlap and TR:O05767 (EMBL:U87307) SigE, extracytoplasmic function alternative sigma factor from Mycobacterium smegmatis (204 aa) fasta scores; opt: 160, z-score: 202.2, E(): 0.00085, 27.8% identity in 187 aa overlap. 2 1 6 19 [Reporter]SCO6606 (4J4)_PCR/632 [Gene]SCO6606/575 NC_003888 SC1F2.03c, unknown, len: 300 aa; similar to upstream gene SC1F3.04, E(): 0, 48.8% identity in 260 aa overlap anbd to TR:Q9L173 (EMBL:AL158057) Streptomyces coelicolor hypothetical protein SC10G8.32c, 263 aa; fasta scores: opt: 791 Z-score: 879.8 E(): 2.3e-41; 49.237% identity in 262 aa overlap 1 2 23 22 [Reporter]SCO4934 (1I23)_PCR/2520 [Gene]SCO4934/2293 NC_003888 SCK13.26, possible lipoprotein, len: 414 aa; similar to TR:Q9KYV1 (EMBL:AL355832) Streptomyces coelicolor putative lipoprotein SCE22.11, 407 aa; fasta scores: opt: 1603 z-score: 1740.6 E(): 0; 60.5% identity in 415 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site 2 1 6 18 [Reporter]SCO6359 (5F4)_PCR/631 [Gene]SCO6359/574 NC_003888 SC3A7.27, possible integral membrane protein, len: 309 aa; similar to hypothetical proteins from M. tuberculosis and M. leprae e.g. M. tuberculosis TR:P95164 (EMBL:Z83859) MTCY359.28 (322 aa), fasta scores; opt: 196 z-score: 298.4 E(): 2.3e-09, 33.7% identity in 267 aa overlap 1 2 23 21 [Reporter]SCO2465 (2E23)_PCR/2519 [Gene]SCO2465/2292 NC_003888 SC7A8.04c, hrdA, RNA polymerase principal sigma factor, len: 396 aa; identical to previously sequenced SW:HRDA_STRCO (EMBL:X52980) Streptomyces coelicolor RNA polymerase principal sigma factor HrdA, 396 aa and highly similar to SW:RPSD_STRAU (EMBL:M90410) Streptomyces aureofaciens RNA polumerase sigma factor RpoD, 317 aa; fasta scores: opt: 1792 z-score: 1762.6 E(): 0; 89.9% identity in 316 aa overlap and to S. coelicolor SC5B8.10, 511 aa; fasta scores: opt: 1276 z-score: 1056.4 E(): 0; 51.6% identity in 397 aa overlap. Contains Pfam match to entry PF00140 sigma70, Sigma-70 factor and two matches to Prosite entries PS00715 Sigma-70 factors family signature 1 and PS00716 Sigma-70 factors family signature 2. Contains also a possible helix-turn-helix motif at residues 355..376 (+6.21 SD) 2 1 6 17 [Reporter]SCO6643 (6B4)_PCR/630 [Gene]SCO6643/573 NC_003888 SC4G2.17, unknown, len: 184 aa; some similarity to TR:O26772 (EMBL:AE000847) hypothetical protein MTH676 (Methanobacterium thermoautotrophicum) (205 aa), fasta scores; opt: 260 z-score: 193.2 E(): 0.0016, 33.1% identity in 163 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family, score 38.50, E-value 1.5e-07 1 2 23 20 [Reporter]SCO7397 (3A23)_PCR/2518 [Gene]SCO7397/2291 NC_003888 SC10G8.25c, unknown, len: 198 aa. Weakly similar to several e.g. Bacillus subtilis TR: O05231(EMBL:Z93933) hypothetical 23.8 KD protein (205 aa), fasta scores opt: 213 z-score: 271.6 E(): 1e-07 28.2% identity in 181 aa overlap. 2 1 6 16 [Reporter]SCO4857 (6N24)_PCR/629 [Gene]SCO4857/572 NC_003888 SC5G8.25c, dhsD, possible succinate dehydrogenase membrane subunit, len: 160 aa; similar to TR:O53369 (EMBL:AL021841) putative membrane anchor of succinate dehydrogenase from Mycobacterium tuberculosis (144 aa) fasta scores; opt: 553, z-score: 702.9, E(): 1.1e-31, 58.9% identity in 141 aa overlap. Also weakly similar to SW:P10445 (DHSD_ECOLI) succinate dehydrogenase hydrophobic membrane anchor protein from Escherichia coli (115 aa) fasta scores; opt: 126, z-score: 172.2, E(): 0.039, 23.5% identity in 115 aa overlap. Contains possible membrane-spanning hydrophobic regions and Pfam match to entry PF01127 Sdh_cyt, Succinate dehydrogenase cytochrome b subunit. 1 2 23 19 [Reporter]SCO7619 (4M19)_PCR/2517 [Gene]SCO7619/2290 NC_003888 SC2H2.17, probable anti sigma factor antagonist, len: 134 aa; similar to SW:RSBV_STRCO (EMBL:AF134889) Streptomyces coelicolor anti-sigma B factor antagonist RsvB or BldG or SCH5.12c, 113 aa; fasta scores: opt: 263 z-score: 348.2 E(): 7.3e-12; 41.2% identity in 97 aa overlap. Contains Pfam match to entry PF01740 STAS, STAS domain 2 1 6 15 [Reporter]SCO2133 (7J24)_PCR/628 [Gene]SCO2133/571 NC_003888 SC6G10.06, possible integral membrane protein, len: 421 aa; similar to hypothetical proteins eg. TR:P71708 (EMBL:Z80775) hypothetical protein from Mycobacterium tuberculosis fasta scores; opt: 230, z-score: 251.3, E(): 1.1e-06, (27.1% identity in 417 aa overlap). Contains possible membrane spanning hydrophobic regions 2 1 6 14 [Reporter]SCO5952 (8F24)_PCR/627 [Gene]SCO5952/570 NC_003888 SC7H1.22, putative membrane protein, len: 250 aa. Contains possible hydrophobic membrane spanning regions 3 4 12 3 [Reporter]SCO4015 (19C17)_PCR/7299 [Gene]SCO4015/6649 NC_003888 2SC10A7.19, unknown, len: 307 aa. Contains a TTA leucine codon. Possible target for bldA regulation 3 4 11 22 [Reporter]SCO1242 (1K9)_PCR/7296 [Gene]SCO1242/6648 NC_003888 2SCG1.17, probable DNA-binding protein, len: 286 aa; similar to TR:CAB8844 (EMBL:AL353815) Streptomyces coelicolor putative DNA-binding protein SCD6.19, 295 aa; fasta scores: opt: 626 z-score: 747.0 E(): 0; 40.0% identity in 280 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and a possible helix-turn-helix motif at residues 25..46 (+4.46 SD). Also contains a TTA leucine codon (58), possible target for bldA regulation 3 4 11 21 [Reporter]SCO1501 (2G9)_PCR/7295 [Gene]SCO1501/6647 NC_003888 SC9C5.25c, alaS, alanine tRNA synthetase, len: 890 aa; similar to SW:SYA_THETH (EMBL:Y08363) Thermus aquaticus alanyl-tRNA synthetase (EC 6.1.1.7) AlaS, 882 aa; fasta scores: opt: 2279 z-score: 2334.6 E(): 0; 44.6% identity in 891 aa overlap. Contains Pfam match to entry PF01411 tRNA-synt_2c, tRNA synthetases class II (A) and match to Prosite entry PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2 3 4 11 20 [Reporter]SCO5425 (3C9)_PCR/7294 [Gene]SCO5425/6646 NC_003888 SC6A11.01c, pta, phosphate acetyltransferase (fragment), len: >655 aa; similar to many e.g. SW:P39184 (PTA_ECOLI) pta, phosphate acetyltransferase from Escherichia coli (713 aa) fasta scores: opt: 1190, z-score: 1306.5, E(): 0, 37.0% identity in 687 aa overlap. Contains Pfam match to entry PF01515 PTA_PTB, Phosphate acetyl/butaryl transferase,SC8F4.29c, partial CDS, pta, phosphate acetyltransferase, len: >69aa; strongly similar to the C-terminus of many eg. SW:P39184 (PTA_ECOLI) pta, phosphate acetyltransferase from Escherichia coli (713 aa) fasta scores; opt: 309, z-score: 427.3, E(): 2.1e-16, 78.3% identity in 60 aa overlap. 3 4 11 19 [Reporter]SCO6620 (4O5)_PCR/7293 [Gene]SCO6620/6645 NC_003888 SC1F2.17, hypothetical protei, len: 143 aa; similar to hypothetical proteins e.g. M. tuberculosis TR:P71885 (EMBL:Z79702) MTCY3G12.07C (163 aa), fasta scores; opt: 171 z-score: 244.4 E(): 2.3e-06, 36.0% identity in 114 aa overlap 3 4 11 18 [Reporter]SCO6574 (5K5)_PCR/7292 [Gene]SCO6574/6644 NC_003888 SC3F9.09, putative membrane protein, len: 363 aa; similar to TR:Q9Z4Y9 (EMBL:AL035707) Streptomyces coelicolor putative transferase SCE29.11c, 291 aa; fasta scores: opt: 666 Z-score: 604.6 E(): 4.8e-26; 51.282% identity in 312 aa overlap. Contains possible hydrophobic membrane spanning regions 3 4 11 17 [Reporter]SCO2037 (6G5)_PCR/7291 [Gene]SCO2037/6643 NC_003888 SC4G6.06c, trpB, tryptophan synthase beta subunit, len: 427 aa; previously sequenced therefore identical to TR:O05625 (EMBL:AF054585). Also similar to SW:TRPB_CAUCR tryptophan synthase beta chain from Caulobacter crescentus (406 aa) fasta scores; opt: 1660, z-score: 1848.0, E(): 0, (61.2% identity in 397 aa overlap). Contains Pfam match to entry PF00247 trp_syntB, Tryptophan synthases, beta chain and Prosite match to PS00168 Tryptophan synthase beta chain pyridoxal-phosphate attachment site. 3 4 11 16 [Reporter]SCO7284 (7C5)_PCR/7290 [Gene]SCO7284/6642 NC_003888 SC5H1.08c, rnhA, probable ribonuclease H, len: 235 aa; similar to many e.g. SW:RNH_THETH (EMBL:X60507), rhnA, Thermus aquaticus ribonuclease H (166 aa), fasta scores; opt: 299 z-score: 312.2 E(): 4.6e-10, 38.0% identity in 166 aa overlap. Also similar to TR:Q54388 (EMBL:X95703), rnhH, Streptomyces lincolnensis putative ribonuclease H (117 aa) (89.7% identity in 117 aa overlap). Has a C-terminal extension not present in the Thermus aquaticus enzyme. Contains Pfam match to entry PF00075 rnaseH, RNase H, score 52.60, E-value 8.9e-12 2 3 17 12 [Reporter]SCO4377 (11J6)_PCR/4899 [Gene]SCO4377/4469 NC_003888 SCD10.09, probable serine-threonine protein kinase, len: 580 aa; similar to SW:AFSK_STRCO (EMBL:D15062) Streptomyces coelicolor serine-threonine protein kinase AfsK (EC 2.7.1.-), 799 aa; fasta scores: opt: 953 z-score: 715.3 E(): 2.3e-32; 43.2% identity in 438 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and matches to Prosite entries PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature. Contains possible hydrophobic membrane spanning region 3 4 11 15 [Reporter]SCO5564 (8O1)_PCR/7289 [Gene]SCO5564/6641 NC_003888 SC7A1.08c, rpmB, 50S ribosomal protein L28, len: 61aa; similar to many including SW:RL28_BACSU RpmB 50S ribosomal protein L28 from Bacillus subtilis (62 aa) fasta scores; opt: 171, z-score: 301.8, E(): 1.6e-09, (41.0% identity in 61 aa overlap). Contains Pfam match to entry PF00830 Ribosomal_L28, Ribosomal L28 family, score 74.50, E-value 2.2e-18. 2 3 17 11 [Reporter]SCO4806 (12F6)_PCR/4898 [Gene]SCO4806/4468 NC_003888 SCD63A.17, possible secreted protein, len: 314 aa. Contains possible N-terminal region signal peptide sequence 3 4 11 14 [Reporter]SCO2641 (9K1)_PCR/7288 [Gene]SCO2641/6640 NC_003888 SC8E4A.11, possible resistance protein, len: 395 aa; similar to TR:Q9ZBZ1 (EMBL:AL034355) Streptomyces coelicolor hypothetical 51.0 kD protein SCD78.06, 482 aa; fasta scores: opt: 715 z-score: 690.5 E(): 4.7e-31; 38.0% identity in 403 aa overlap and to SW:TERD_ALCSP (EMBL:M20238) Alcaligenes sp. tellurium resistance protein TerD, 192 aa; fasta scores: opt: 392 z-score: 388.1 E(): 3.3e-14; 40.2% identity in 189 aa overlap 2 3 17 10 [Reporter]SCO3070 (13B6)_PCR/4897 [Gene]SCO3070/4467 NC_003888 SCE25.11c, probable chlorohydrolase, len: 391 aa; similar to SW:HUTI_BACSU (EMBL:D31856) Bacillus subtilis imidazolonepropionase (EC 3.5.2.7) (imidazolone-5-propionate hydrolase) HutI, 421 aa; fasta scores: opt: 659 z-score: 767.1 E(): 0; 36.1% identity in 404 aa overlap. Contains Pfam match to entry PF01685 ATZ_TRZ, Chlorohydrolase 2 3 17 9 [Reporter]SCO0234 (14N2)_PCR/4896 [Gene]SCO0234/4466 NC_003888 SCJ9A.13, probable oxidoreductase, len: 153aa; similar to many eg. SW:DHG_BACME glucose dehydrogenase from Bacillus megaterium (261 aa) fasta scores; opt: 252, z-score: 277.8, E(): 4.1e-08, (44.0% identity in 100 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase 2 3 17 8 [Reporter]SCO3574 (15J2)_PCR/4895 [Gene]SCO3574/4465 NC_003888 SCH17.08c, possible epoxide hydrolase, len: 354 aa; similar to eukaryotic epoxide hydrolases e.g. TR:Q42566 (EMBL:D16628), atseH, Arabidopsis thaliana epoxide hydrolase (321 aa), fasta scores; opt: 501 z-score: 542.6 E(): 6.8e-23, 37.7% identity in 332 aa overlap. Similar to others from S.coelicolor e.g. SCH5.29 (EMBL:AL035636) putative hydrolase (324 aa) (33.6% identity in 348 aa overlap). Alternative start codons are present at codons 6 and 30, but these have no apparent RBS. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 2 3 17 7 [Reporter]SCO0087 (16F2)_PCR/4894 [Gene]SCO0087/4464 NC_003888 SCJ11.16c, putative secreted protein, len: 349 aa; similar to TR:P97061 (EMBL:Z81324) hypothetical protein downstream of a beta-lactam regulatory protein in Streptomyces clavuligerus (338 aa) fasta scores; opt: 548, z-score: 613.6, E(): 8.1e-27, (40.6% identity in 330 aa overlap) 2 3 17 6 [Reporter]SCO3674 (17B2)_PCR/4893 [Gene]SCO3674/4463 NC_003888 SCH35.50c, possible sensor kinase, len: 433 aa; similar to TR:O06213 (EMBL:Z95388) hypothetical protein from Mycobacterium tuberculosis (384 aa) fasta scores; opt: 176, z-score: 174.4, E(): 0.022, (31.0% identity in 210 aa overlap) and SW:PKAA_STRCO serine/threonine protein kinase from Streptomyces coelicolor (543 aa) fasta scores; opt: 160, z-score: 157.4, E(): 0.19, (29.4% identity in 262 aa overlap). Contains possible N-terminal signal sequence and two Prosite matches to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 1 2 23 18 [Reporter]SCO6711 (5I19)_PCR/2516 [Gene]SCO6711/2289 NC_003888 SC4C6.21c, hypothetical protein, len: 781 aa; unknown function, C-terminal half similar to N-terminal region of TR:O69947 (EMBL:AL023862) S.coelicolor hypothetical protein (1238 aa), fasta scores; opt: 818 z-score: 850.9 E(): 0, 38.5% identity in 535 aa overlap. The downstream ORF SC4C6.20c has weak similarity to the C-terminal half of this hypothetical protein 2 3 17 5 [Reporter]SCO0428 (17N22)_PCR/4892 [Gene]SCO0428/4462 NC_003888 SCF51A.06, possible tetR family transcriptional regulator, len: 177 aa. Similar to many putative regulators e.g. Streptomyces coelicolor TR:Q9ZBU4 (EMBL; AL034446) putative transcriptional regulator SC1A9.08C (189 aa), fasta scores opt: 402 z-score: 476.9 E(): 3.3e-19 45.5% identity in 165 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a possible helix-turn-helix motif between residues 18..39 (+2.91 SD). 1 2 23 17 [Reporter]SCO7563 (6E19)_PCR/2515 [Gene]SCO7563/2288 NC_003888 SC5F1.17, possible ABC transporter solute binding lipoprotein, len: 516 aa; similar to TR:Q9KFX3 (EMBL:AP001508) Bacillus halodurans oligopeptide ABC transporter AppA, 516 aa; fasta scores: opt: 708 z-score: 807.2 E(): 0; 29.7% identity in 482 aa overlap. Contains 2x Pfam matches to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5 and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal signal peptide sequence 2 3 17 4 [Reporter]SCP1.297 (18J22)_PCR/4891 [Gene]SCP1.297/4461 NC_003888 SCP1.297, spore associated protein, SapC, len: 150aa; similar to TR:Q9KYK7 (EMBL:AL356593) putative secreted protein from Streptomyces coelicolor (155 aa) fasta scores; opt: 346, z-score: 450.7, E(): 1.2e-17, 40.5% identity in 131 aa overlap. Contains a possible cleavable N-terminal signal sequence. 2 1 6 13 [Reporter]SCO6073 (9B24)_PCR/626 [Gene]SCO6073/569 NC_003888 SC9B1.20, possible cyclase, len: 726 aa; C-terminal region similar to SW:PTLS_STRSQ pentalenene synthase from Streptomyces UC5319 (336 aa) fasta scores; opt: 382, z-score: 446.4, E(): 1.5e-17, (28.9% identity in 339 aa overlap). 1 2 23 16 [Reporter]SCO2676 (7A19)_PCR/2514 [Gene]SCO2676/2287 NC_003888 SC6D10.19c, hypothetical protein, len: 136 aa; similar to various hypothetical proteins, e.g. TR:O53202 (EMBL:AL021246) Mycobacterium tuberculosis hypothetical 15.8 kD protein MTV008.31c, 138 aa; fasta scores: opt: 236 z-score: 318.7 E(): 2.4e-10; 34.1% identity in 126 aa overlap 2 3 17 3 [Reporter]SCO1672 (19F22)_PCR/4890 [Gene]SCO1672/4460 NC_003888 SCI52.14c, unknown, len: 267 aa. Contains 6x degenerate repeat unit:(T/G)(D/E)LD 2 1 6 12 [Reporter]SCO2747 (10N20)_PCR/625 [Gene]SCO2747/568 NC_003888 SCC57A.18, bifunctional carbohydrate binding and transport protein, len: 613 aa. The N-terminus is highly similar to many ribose transport system permeases e.g. Escherichia coli SW:RBSC_ECOLI(EMBL:L10328) ribose transport system permease protein RbsC (321 aa), fasta scores opt: 846 z-score: 833.8 E(): 0 47.1% identity in 310 aa overlap. The C-terminus is highly similar to many ribose-binding proteins e.g. Escherichia coli SW:RBSB_ECOLI(EMBL:K00511) D-ribose-binding periplasmic protein precursor (296 aa), fasta scores opt: 863 z-score: 847.0 E():0 46.9% identity in 303 aa overlap. Contains a Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family. The N-terminus contains multiple possible membrane spanning hydrophobic domains. 1 2 23 15 [Reporter]SCO2002 (8M15)_PCR/2513 [Gene]SCO2002/2286 NC_003888 SC7H2.16c, unknown, len: 118aa; 2 1 6 11 [Reporter]SCO4629 (11J20)_PCR/624 [Gene]SCO4629/567 NC_003888 SCD39.29, hypothetical protein, len: 221 aa; identical to previously sequenced SW:YIMC_STRCO (EMBL:X15866) Streptomyces coelicolor hypothetical 22.7 kDa protein in Imp region (ORFC), 203 aa. Contains possible alternative translational starting point downstream 1 2 23 14 [Reporter]SCO5857 (9I15)_PCR/2512 [Gene]SCO5857/2285 NC_003888 SC9B10.24c, probable FAD-dependent oxidoreductase, len: 445 aa; similar to several eg. GGLO_RAT P10867 l-gulonolactone oxidase (ec 1.1.3.8) (439 aa), fasta scores; opt: 854 z-score: 973.6 E(): 0, 33.6% identity in 440 aa overlap. Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site, and possible transmembrane anchor at approx. 170-200 aa 2 1 6 10 [Reporter]SCO4307 (12F20)_PCR/623 [Gene]SCO4307/566 NC_003888 SCD95A.40c, hypothetical protein, len: 311 aa; similar to SW:YFEU_ECOLI (EMBL:AE000330) Escherichia coli hypothetical 31.2 kD protein in CysP-AmiA intergenic region YfeU, 298 aa; fasta scores: opt: 973 z-score: 1053.7 E(): 0; 53.4% identity in 294 aa overlap 1 2 23 13 [Reporter]SCO2434 (10E15)_PCR/2511 [Gene]SCO2434/2284 NC_003888 SCC24.05c, possible sugar transporter sugar binding protein, len: 452 aa; similar to TR:CAB59595 (EMBL:AL132662) Streptomyces coelicolor, probable sugar transporter sugar binding lipoprotein SCF11.18, 442 aa; fasta scores: opt: 274 z-score: 320.2 E(): 2.1e-10; 23.3% identity in 443 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein. Contains possible N-terminal signal peptide sequence 2 1 6 9 [Reporter]SCO5051 (13B20)_PCR/622 [Gene]SCO5051/565 NC_003888 SCK7.24, possible glycosyltransferase, len: 487 aa; similar to SW:NODC_RHIME (EMBL:X01649) Rhizobium meliloti N-acetylglucosaminyltransferase (EC 2.4.1.-) NodC, 426 aa; fasta scores: opt: 377 z-score: 447.1 E(): 2e-17; 29.6% identity in 433 aa overlap. Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases and possible hydrophobic membrane spanning regions 1 2 23 12 [Reporter]SCO4106 (11A15)_PCR/2510 [Gene]SCO4106/2283 NC_003888 SCD17.10, possible bifunctional enzyme deaminase/reductase, len: 376 aa. Similar to both domains of the Escherichia coli bifunctional SW:RIBD_ECOLI (EMBL; X64395) riboflavin biosynthesis protein RibD (367 aa). However the N-terminal domain of SCD17.10 is similar to the E. coli C-terminal reductase domain, fasta scores opt: 209 z-score: 241.1 E(): 4.5e-06 27.0% identity in 222 aa overlap and the C-terminal domain of SCD17.10 is similar to the E. coli N-terminal deaminase domain (blast scores Expect = 3.2e-07, P = 3.2e-07, Identities = 51/192 (26%), Positives = 77/192 (40%)). Also similar to other, hypothetical, deaminases e.g. Archaeoglobus fulgidus TR:O28272 riboflavin-specific deaminase (RibG) (219 aa), fasta scores opt: 410 z-score: 471.2 E(): 6.8e-19 38.0% identity in 205 aa overlap. 2 1 6 8 [Reporter]SCO0225 (14N16)_PCR/621 [Gene]SCO0225/564 NC_003888 SCJ9A.04c, unknown, len: 144aa; predicted by amino acid usage. 1 2 23 11 [Reporter]SCO4160 (12M11)_PCR/2509 [Gene]SCO4160/2282 NC_003888 SCD84.27, possible hydrolase, len: 278 aa; similar to TR:AAF25687 (EMBL:AF218823) Thermomonospora fusca putative dienelactone hydrolase DlhA, 265 aa; fasta scores: opt: 786 z-score: 885.1 E(): 0; 50.0% identity in 262 aa overlap 2 1 6 7 [Reporter]SCO5001 (15J16)_PCR/620 [Gene]SCO5001/563 NC_003888 SCK15.03, possible integral membrane protein, len: 213 aa; similar to TR:CAB77310 (EMBL:AL160312) Streptomyces coelicolor putative integral membrane protein SCD35.26, 198 aa; fasta scores: opt: 349 z-score: 424.5 E(): 3.6e-16; 37.4% identity in 182 aa overlap. Contains possible hydrophobic membrane spanning regions at C-terminal domain 1 2 23 10 [Reporter]SCO3122 (13I11)_PCR/2508 [Gene]SCO3122/2281 NC_003888 SCE66.01, probable nucleotidyltransferase, partial CDS, len: >343 aa; similar to many eg.. SW:P14192 (GCAD_BACSU) UDP-N-acetylglucosamine pyrophosphorylase from Bacillus subtilis (456 aa) fasta scores; opt: 895, z-score: 1020.4, E(): 0, 42.6% identity in 317 aa overlap. Contains five Pfam matches to entry PF00132 hexapep, Bacterial transferase hexapeptide.,SCE41.31, probable nucleotidyltransferase (fragment), len: >174 aa; similar to SW:GLMU_ECOLI (EMBL:X01631) Escherichia coli UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23) GlmU, 456 aa; fasta scores: opt: 369 z-score: 411.5 E(): 2.1e-15; 42.4% identity in 165 aa overlap 2 1 6 6 [Reporter]SCO5156 (16F16)_PCR/619 [Gene]SCO5156/562 NC_003888 SCP8.19c, unknown, len: 170 aa 1 2 23 9 [Reporter]SCO3673 (14E11)_PCR/2507 [Gene]SCO3673/2280 NC_003888 SCH35.51c, probable iron-sulphur-binding reductase, len: 433 aa; similar to many eg. TR:P96797 (EMBL:Y09870) heterodisulphide reductase from Methanosarcina barkeri (409 aa) fasta scores; opt: 427, z-score: 463.9, E(): 1.6e-18, (28.9% identity in 443 aa overlap). Contains 2 Pfam matches to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains and 2 Prosite matches to PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. Contains also possible hydrophobic membrane spanning regions 2 1 6 5 [Reporter]SCO4955 (17B16)_PCR/618 [Gene]SCO4955/561 NC_003888 2SCK31.15c, hypothetical protein, len: 395 aa; similar to TR:Q9X8V7 (EMBL:AL078610) Streptomyces coelicolor hypothetical 43.9 kDa protein SCH35.49c, 410 aa; fasta scores: opt: 1788 z-score: 1973.1 E(): 0; 63.8% identity in 398 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 6 4 [Reporter]SCO2292 (18N12)_PCR/617 [Gene]SCO2292/560 NC_003888 SCC75A.38, xlnB, secreted endo-1,4-beta-xylanase B (xylanase B), len: 335 aa. Almost identical to Streptomyces lividans SW:XYNB_STRLI(EMBL:M64552) endo-1,4-beta-xylanase B precursor (xylanase B) (EC 3.2.1.8) (334 aa), fasta scores opt: 2266 z-score: 2249.3 E():0 98.2% identity in 335 aa overlap. Also similar to Streptomyces coelicolor TR:CAB52919 (EMBL:AL109949) endo-1,4-beta-xylanase, XlnC (241 aa), fasta scores opt: 1087 z-score: 970.6 E():0 71.9% identity in 224 aa overlap and to the adjoining CDS on this cosmid, AxeA, (335 aa), fasta scores opt: 652 z-score: 585.8 E(): 2.9e-27 56.2% identity in 201 aa overlap. Contains a N-terminal signal sequence, Prosite hits to PS00776 Glycosyl hydrolases family 11 active site signature 1 and PS00777 Glycosyl hydrolases family 11 active site signature 2, as well as a Pfam match to entry PF00457 Glyco_hydro_11, Glycosyl hydrolases family 11. 3 4 11 13 [Reporter]SCO6480 (10G1)_PCR/7287 [Gene]SCO6480/6639 NC_003888 SC9C7.16c, unknown, len: 99 aa 3 4 11 12 [Reporter]SCO2704 (11C1)_PCR/7286 [Gene]SCO2704/6638 NC_003888 SCC61A.25c, possible secreted protein, len: 244aa; contains a possible cleavable N-terminal signal sequence. 3 4 11 11 [Reporter]SCO4673 (11O21)_PCR/7285 [Gene]SCO4673/6637 NC_003888 SCD40A.19c, possible deoR-family transcriptional regulator, len: 339 aa; similar to TR:Q54822 (EMBL:L37338) Streptomyces peucetius putative repressor DnrO, 340 aa; fasta scores: opt: 660 z-score: 749.1 E(): 0; 38.7% identity in 333 aa overlap. Contains match to Prosite entry PS00894 Bacterial regulatory proteins, deoR family signature and possible helix-turn-helix motif at residues 27..48 (+5.18 SD) 3 4 11 10 [Reporter]SCO3528 (12K21)_PCR/7284 [Gene]SCO3528/6636 NC_003888 SCE2.09, unknown, len: 386 aa; weak similarity to several predicted helicases e.g. SW:DNAB_TREPA replicative DNA helicase from Treponema pallidum (438 aa) fasta scores; opt: 188, z-score: 190.6, E(): 0.0027, (36.5% identity in 156 aa overlap) and similar to TR:Q9L1W8 (EMBL:AL138667) Streptomyces coelicolor hypothetical protein SC3D9.11c, 372 aa; fasta scores: opt: 559 Z-score: 528.6 E(): 8.2e-22; 37.466% identity in 363 aa overlap 3 4 11 9 [Reporter]SCO1647 (13G21)_PCR/7283 [Gene]SCO1647/6635 NC_003888 SCI41.30c, hypothetical protein, len: 497 aa. Identical to TR:O87595 (EMBL:AF086832) Streptomyces coelicolor hypothetical 65.1 Kd protein, len: 497 aa and similar to TR:Q53081 (EMBL:U26422) Rhodococcus sp. similar to ORF6(1) of Rhodococcus sp. NI86/21, 499 aa; fasta scores: opt: 2019 z-score: 2370.6 E():0; 62.5% identity in 493 aa overlap. Contains also similarities with Streptomyces coelicolor StI41.23c, 453 aa; fasta scores: opt: 962 z-score: 1019.2 E():0; 41.9% identity in 413 aa overlap 3 4 11 8 [Reporter]SCO0488 (14C21)_PCR/7282 [Gene]SCO0488/6634 NC_003888 SCF34.07, putative hydrolase, len: 1138 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Similar to TR:Q9S3L0 (EMBL:AJ238748) alfa-L-ramnisidase from Clostridium stercorarium (874 aa) fasta scores; opt: 554, Z-score: 585.8, E(): 5.4e-25, 27.513% identity (30.672% ungapped) in 796 aa overlap. 3 4 11 7 [Reporter]SCO5025 (15O17)_PCR/7281 [Gene]SCO5025/6633 NC_003888 SCK15.27, possible transcriptional regulator, len: 305 aa; similar to TR:Q9RJS9 (EMBL:AL132707) Streptomyces coelicolor putative transcriptional regulator SCF51.06 334 aa; fasta scores: opt: 166 z-score: 190.7 E(): 0.0037; 27.3% identity in 289 aa overlap and to SW:TIPA_STRLI (EMBL:S64314) Streptomyces lividans transcriptional activator TipA, 253 aa; fasta scores: opt: 174 z-score: 201.3 E(): 0.00096; 35.5% identity in 107 aa overlap. Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and possible helix-turn-helix at residues 11..32 (+2.74 SD) 3 4 11 6 [Reporter]SCO0244 (16K17)_PCR/7280 [Gene]SCO0244/6632 NC_003888 SCJ9A.23, putative secreted protein, len: 336 aa; similar to TR:O88063 (EMBL:AL031541) putative secreted protein from Streptomyces coelicolor (322 aa) fasta scores; opt: 852, z-score: 891.5, E(): 0, (51.9% identity in 320 aa overlap) 2 3 16 22 [Reporter]SCO1211 (1N14)_PCR/4887 [Gene]SCO1211/4459 NC_003888 2SCG58.11c, probable polypeptide deformylase, len: 217 aa; similar to SW:DEF_STRCO (EMBL:AL078618) Streptomyces coelicolor polypeptide deformylase (EC 3.5.1.31) Def or SC10A7.23, 208 aa; fasta scores: opt: 594 z-score: 712.9 E(): 3.1e-32; 54.2% identity in 166 aa overlap and to SW:DEF_ECOLI (EMBL:X77800) Escherichia coli polypeptide deformylase (EC 3.5.1.31) Def, 168 aa; fasta scores: opt: 321 z-score: 391.3 E(): 2.5e-14; 34.2% identity in 155 aa overlap. Contains Pfam match to entry PF01327 Pep_deformylase, Polypeptide deformylase 3 4 11 5 [Reporter]SCO1607 (17G17)_PCR/7279 [Gene]SCO1607/6631 NC_003888 SCI35.29c, probable serine protease (putative membrane protein), len: 393 aa; similar to many members of the subtilase family e.g. SUBT_BACLI subtilisin carlsberg precursor (379 aa), fasta scores; opt: 377 z-score: 509.5 E(): 3.9e-21, 38.8% identity in 276 aa overlap. Contains probable possible hydrophobic membrane spanning regions (one possible hydrophobic span at the N-terminal region and another one at the C-terminal region), PS00136 Serine proteases, subtilase family, aspartic acid active sitea and Pfam match to entry PF00082 subtilase, Subtilase family of serine proteases, score 177.60, E-value 2.1e-49 2 3 16 21 [Reporter]SCO7660 (2J14)_PCR/4886 [Gene]SCO7660/4458 NC_003888 SC10F4.33, kcsA, voltage-gated potassium channel, len: 160 aa; identical to SW:KCSA_STRLI (EMBL:Z37969) Streptomyces lividans voltage-gated potassium channel KcsA, 160 aa. Contains possible hydrophobic membrane spanning regions 3 4 11 4 [Reporter]SCO1846 (18C17)_PCR/7278 [Gene]SCO1846/6630 NC_003888 SCI8.31, possible membrane protein, len: 73 aa. Contains possible hydrophobic membrane spanning regions 2 3 16 20 [Reporter]SCO2222 (3F14)_PCR/4885 [Gene]SCO2222/4457 NC_003888 SC10B7.17, probable iron-sulfur oxidoreductase (putative secreted protein), len: 364 aa; similar to TR:O88034 (EMBL:AL031107) Streptomyces coelicolor iron-sulfur oxidoreductase beta subunit SC5A7.28c, 313 aa; fasta scores: opt: 1032 z-score: 1177.8 E(): 0; 55.2% identity in 310 aa overlap and to SW:POBB_PSEPS (EMBL:X78823) Pseudomonas pseudoalcaligenes phenoxybenzoate dioxygenase beta subunit (EC 1.-.-.-.) PobB, 319 aa; fasta scores: opt: 680 z-score: 777.9 E(): 0; 40.8% identity in 314 aa overlap. Contains two matches to Pfam entries Pfam match to entry PF00175 oxidored_fad, Oxidoreductase FAD/NAD-binding and Pfam match to entry PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains domain match to Prosite entry PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Contains possible N-terminal region signal peptide sequence 2 3 16 19 [Reporter]SCO5481 (4B14)_PCR/4884 [Gene]SCO5481/4456 NC_003888 SC2A11.15, probable integral membrane protein, len: 219 aa; similar to hypothetical proteins e.g. YADS_ECOLI (207 aa), fasta scores; opt: 397 z-score: 543.4 E(): 5.2e-23, 36.7% identity in 196 aa overlap 2 3 16 18 [Reporter]SCO2095 (5N10)_PCR/4883 [Gene]SCO2095/4455 NC_003888 SC4A10.28, possible membrane protein, len: 454 aa; unknown function, similar to parts of bacterial hypothetical proteins e.g. TR:P74479 (EMBL:D90915) Synechocystis sp hypothetical protein (406 aa), fasta scores; opt: 266 z-score: 307.9 E(): 8e-10, 30.4% identity in 253 aa overlap. Contains hydrophobic, possible membrane-spanning regions near the N-terminus 2 3 16 17 [Reporter]SCO6518 (6J10)_PCR/4882 [Gene]SCO6518/4454 NC_003888 SC5C7.03, probable transcriptional regulator, len: 160 aa; similar in N-terminus to many e.g. RAMA_ENTCL transcriptional activator RamA (113 aa), fasta scores; opt: 175 z-score: 255.3 E(): 5.7e-07, 32.3% identity in 96 aa overlap. C-terminal 50 aa are unique. Contains Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family, score 71.40, E-value 1.9e-17 2 3 16 16 [Reporter]SCO5689 (7F10)_PCR/4881 [Gene]SCO5689/4453 NC_003888 SC5H4.13, probable beta-galactosidase, len: 656 aa; similar to SW:BGAL_BACST (EMBL:M13466) Bacillus stearothermophilus beta-galactosidase I (EC 3.2.1.23) (lactase) BgaB, 672 aa; fasta scores: opt: 1761 z-score: 2065.7 E(): 0; 41.3% identity in 634 aa overlap and to TR:CAB76326 (EMBL:AL158061) Streptomyces coelicolor probable beta-galactosidase SC6D11.03c, 681 aa; fasta scores: opt: 1739 z-score: 1719.0 E(): 0; 46.0% identity in 670 aa overlap 1 2 23 8 [Reporter]SCO0912 (15A11)_PCR/2506 [Gene]SCO0912/2279 NC_003888 SCM1.45, unknown, len: 271 aa. Similar to several proteins of undefined function including: Mycobacterium tuberculosis TR:O69670(EMBL:AL022121) hypothetical 24.6 KD protein (233 aa), fasta scores opt: 383 z-score: 443.9 E(): 2.2e-17 44.8% identity in 252 aa overlap. Also weakly similar to several Eukaryotic proteins e.g. Saccharomyces cerevisiae SW:YNT1_YEAST(EMBL:Z71467) hypothetical 40.2 KD protein (357 aa), fasta scores opt: 221 z-score: 257.3 E(): 5.5e-07 26.1% identity in 306 aa overlap. 2 3 16 15 [Reporter]SCO5313 (8B10)_PCR/4880 [Gene]SCO5313/4452 NC_003888 SC6G9.20, possible membrane protein, len: 303 aa; unknown function, C-terminal half shows weak similarity to the corresponding region of TR:O05859 (EMBL:Z95120) Mycobacterium tuberculosis hypothetical protein (285 aa), fasta scores; opt: 285 z-score: 275.6 E(): 5e-08, 31.4% identity in 277 aa overlap. Contains a hydrophobic, possible membrane-spanning region near the N-terminus. Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature 1 2 23 7 [Reporter]SCO0454 (16M7)_PCR/2505 [Gene]SCO0454/2278 NC_003888 SCF51A.32, probable binding-protein dependent transport protein, len: 313 aa. Similar to many including: Streptomyces coelicolor TR:CAB41203 (EMBL; AL049661) putative protein SCE134.05C (327 aa), fasta scores opt: 479 z-score: 546.7 E(): 4.3e-23 31.4% identity in 315 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and a Prosite hit to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains multiple possible membrane spanning hydrophobic domains. 2 3 16 14 [Reporter]SCO7838 (9N6)_PCR/4879 [Gene]SCO7838/4451 NC_003888 SC8E7.35, unknown, len: 111 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1Y5(EMBL:AL109973) hypothetical protein, SCJ30.04c (111 aa). 2 1 6 3 [Reporter]SCP1.150c (19J12)_PCR/616 [Gene]SCP1.150c/559 NC_003888 SCP1.150c, unknown, len: 369aa; 1 2 23 6 [Reporter]SCO1793 (17I7)_PCR/2504 [Gene]SCO1793/2277 NC_003888 SCI5.01, partial CDS, unknown, len: >195aa; similar to TR:O87735 (EMBL:D89936) sporulation control protein Spo0M from Bacillus subtilis (258 aa) fasta scores; opt: 283, z-score: 342.4, E(): 9.7e-12, (32.7% identity in 153 aa overlap).,SCI51.33, hypothetical protein, partial CDS, len: >98 aa; unknown function, similar to TR:O87735 (EMBL:D89936), Spo0M, Bacillus subtilis sporulation-control protein (258 aa), fasta scores; opt: 214 z-score: 268.2 E(): 1.4e-07, 34.8% identity in 92 aa overlap. Continues in cosmid I5 as SCI5.01 (EMBL:AL079332) S.coelicolor hypothetical protein (195 aa). Contains possible N-terminal region signal peptide sequence 2 3 16 13 [Reporter]SCO2533 (10J6)_PCR/4878 [Gene]SCO2533/4450 NC_003888 SCC117.06, unknown, len: 165 aa. Highly similar to several proteins of unknown function, including: Mycobacterium tuberculosis SW:Y1DD_MYCTU(EMBL:Z95208) hypothetical 20.3 KD protein (182 aa), fasta scores opt: 599 z-score: 713.5 E(): 2.4e-32 56.5% identity in 154 aa overlap and Mycobacterium leprae SW:YN67_MYCLE(EMBL:U00016) hypothetical 19.8 KD protein (178 aa), fasta scores opt: 588 z-score: 700.8 E(): 1.2e-31 54.5% identity in 154 aa overlap. Contains a Prosite hit to PS01306 Uncharacterised protein family UPF0054 signature 2 1 6 2 [Reporter]SCP1.211c (20B12)_PCR/615 [Gene]SCP1.211c/558 NC_003888 SCP1.211c, unknown, len: 143aa; 1 2 23 5 [Reporter]SCO3914 (18E7)_PCR/2503 [Gene]SCO3914/2276 NC_003888 SCH24.36c, probable transcriptional regulator, len: 154 aa; weakly similar to SW:PECS_ERWCH (EMBL:X74409) PecS, Erwinia chrysanthemi regulator of pectinase, cellulase, and blue pigment production (166 aa), fasta scores; opt: 136 z-score: 183.2 E(): 0.0068, 30.5% identity in 95 aa overlap. Also similar to other putative regulatory proteins. Contains Pfam match to entry PF01047 MarR, MarR family, score 52.20, E-value 1.1e-11 2 1 5 22 [Reporter]SCO5248 (1B8)_PCR/613 [Gene]SCO5248/557 NC_003888 2SC7G11.10c, hypothetical protein, len: 192 aa; similar to TR:O06288 (EMBL:Z95557) Mycobacterium tuberculosis hypothetical 30.6 kDa protein MTCY07H7.28c, 275 aa; fasta scores: opt: 180 z-score: 221.9 E(): 6.8e-05; 31.8% identity in 176 aa overlap 1 2 23 4 [Reporter]SCO4016 (19A7)_PCR/2502 [Gene]SCO4016/2275 NC_003888 2SC10A7.20, hypothetical protein, len: 317 aa; low similarity to TR:Q9XDF3 (EMBL:AF074603) Streptomyces griseus NonC protein, 317 aa; fasta scores: opt: 202 z-score: 235.7 E(): 1.3e-05; 26.9% identity in 238 aa overlap 2 1 5 21 [Reporter]SCO1188 (2N4)_PCR/612 [Gene]SCO1188/556 NC_003888 SCG11A.19, celS2, secreted cellulose binding protein len: 364 aa; highly similar to TR:AAD27623 (EMBL:AF126376) Streptomyces viridosporus cellulose binding protein CelS2, 358 aa; fasta scores: opt: 2109 z-score: 2160.9 E(): 0; 81.9% identity in 364 aa overlap and to SW:GUNA_MICBI Microbispora bispora endoglucanase A precursor (EC 3.2.1.4) (endo-1,4-beta-glucanase) (CELLULASE) CelA, 456 aa; fasta scores: opt: 350 z-score: 363.9 E(): 6.8e-13; 30.6% identity in 278 aa overlap. Contains match to Pfam entry PF00553 CBD_2, Cellulose binding domain. Contains also possible signal peptide sequence 1 2 23 3 [Reporter]SCP1.217Ac (20M3)_PCR/2501 [Gene]SCP1.217Ac/2274 NC_003888 SCP1.217Ac, unknown, len: 381aa; flanked by inverted repeat at 217861-217881 and 219205-219225 (Score 56: 20/21 ( 95%) matches, 0 gaps). 2 1 5 20 [Reporter]SCO4709 (3J4)_PCR/611 [Gene]SCO4709/555 NC_003888 SCD31.34, rplP, 50S ribosomal protein L16, len: 139 aa; highly similar to SW:RL16_BACSU (EMBL:D50302) Bacillus subtilis 50S ribosomal protein L16 RplP, 144 aa; fasta scores: opt: 625 z-score: 768.2 E(): 0; 63.8% identity in 141 aa overlap. Contains Pfam match to entry PF00252 Ribosomal_L16, Ribosomal protein L16 and match to Prosite entry PS00701 Ribosomal protein L16 signature 2 1 2 22 22 [Reporter]SCO1092 (1E23)_PCR/2499 [Gene]SCO1092/2273 NC_003888 2SCG4.08, conserved hypothetical protein, len: 130 aa; similar to TR:O06412 (EMBL:Z95558;) Mycobacterium tuberculosis hypothetical 14.3 kD protein MTCY25D10.25c, 128 aa; fasta scores: opt: 399 z-score: 498.2 E(): 2.9e-20; 48.4% identity in 128 aa overlap 2 1 5 19 [Reporter]SCO6916 (4F4)_PCR/610 [Gene]SCO6916/554 NC_003888 SC1B2.22c, putative secreted protein, len: 230 aa. Contains possible N-terminal region signal peptide sequence 1 2 22 21 [Reporter]SCO2778 (2A23)_PCR/2498 [Gene]SCO2778/2272 NC_003888 SCC105.09, hmgL, hydroxymethylglutaryl-CoA lyase, len: 317 aa; similar to TR:P95639 (EMBL:U41280) Rhodospirillum rubrum hydroxymethylglutaryl-CoA lyase (EC4.1.3.4) HmgL, 303 aa; fasta scores: opt: 924 z-score: 1043.1 E(): 0; 51.2% identity in 297 aa overlap and to SW:HMGL_RAT (EMBL:Y10054) Rattus norvegicus hydroxymethylglutaryl-CoA lyase, mitochondrial precursor (EC 4.1.3.4) HmgCL, 325 aa; fasta scores: opt: 983 z-score: 1108.8 E(): 0; 52.7% identity in 296 aa overlap. Contains Pfam match to entry PF00682 HMGL-like, HMGL-like 2 1 5 18 [Reporter]SCO6354 (5B4)_PCR/609 [Gene]SCO6354/553 NC_003888 SC3A7.22c, probable two-component regulator, len: 204 aa; similar to many e.g. CUTR_STRLI transcriptional regulatory protein CutR (217 aa), fasta scores; opt: 386 z-score: 526.1 E(): 4.7e-22, 38.1% identity in 202 aa overlap. Contains Pfam matches to entry PF00486 trans_reg_C, Transcriptional regulatory proteins, C terminal, score 69.70, E-value 1.8e-19 and to entry PF00072 response_reg, Response regulator receiver domain, score 45.60, E-value 1.1e-09 1 2 22 20 [Reporter]SCO6431 (3M19)_PCR/2497 [Gene]SCO6431/2271 NC_003888 SC1A6.20, probable peptide synthase, len: 1171 aa; similar to many e.g. TR:Q44103 (EMBL:X97860) Amycolatopsis mediterranei aps gene for peptide synthetase (1324 aa), fasta scores; opt: 379 z-score: 1175.7 E(): 0, 32.3% identity in 993 aa overlap. Contains PS00455 Putative AMP-binding domain signature, PS00012 Phosphopantetheine attachment site and Pfam match to entry AMP-binding PF00501, AMP-binding enzymes, score 421.02 2 1 5 17 [Reporter]SCO7325 (5N24)_PCR/608 [Gene]SCO7325/552 NC_003888 SC4G10.04c, anti-sigma factor antagonist, len: 130 aa. Similar to many including: Bacillus licheniformis SW:SP21_BACLI (EMBL:M25260) anti-sigma F factor antagonist (stage II sporulation protein AA) (117 aa), fasta scores opt: 187 z-score: 252.1 E(): 1.4e-06 28.4% identity in 109 aa overlap and Streptomyces coelicolor SW:RSBV_STRCO (EMBL:AF134889) anti-sigma factor antagonist, BldG (113 aa), fasta scores opt: 196 z-score: 263.7 E(): 3.2e-07 26.5% identity in 102 aa overlap. Contains a Pfam match to entry PF01740 SpoIIAA, SpoIIAA family. 1 2 22 19 [Reporter]SCO7615 (4I19)_PCR/2496 [Gene]SCO7615/2270 NC_003888 SC2H2.13, possible DNA-binding protein, len: 290 aa; similar to many Streptomyces coelicolor putative DNA-binding proteins, e.g. TR:CAC01466 (EMBL:AL391014) putative DNA-binding protein 2SCG1.17, 286 aa; fasta scores: opt: 613 z-score: 722.6 E(): 1e-32; 41.8% identity in 261 aa overlap and TR:CAA20634 (EMBL:AL031515) hypothetical protein SC5C7.22c, 287 aa; fasta scores: opt: 917 z-score: 1027.9 E(): 0; 50.0% identity in 292 aa overlap. Contains possible helix-turn-helix motif at residues 33..54 (+4.70 SD) 2 1 5 16 [Reporter]SCO4851 (6J24)_PCR/607 [Gene]SCO4851/551 NC_003888 SC5G8.19c, unknown, len: 162aa; 2 1 5 15 [Reporter]SCO1431 (7F24)_PCR/606 [Gene]SCO1431/550 NC_003888 SC6D7.08, possible membrane protein, len: 80 aa. Contains a possible membrane spanning hydrophobic domain. 3 4 11 3 [Reporter]SCP1.19c (19O13)_PCR/7277 [Gene]SCP1.19c/6629 NC_003888 SCP1.19c, possible DNA integrase/recombinase, len: 408aa; C-terminal region weakly similar to many members of the 'phage' integrase family eg. SW:P21891 (XERD_ECOLI) integrase/recombinase XerD from Escherichia coli (298 aa) fasta scores; opt: 195, z-score: 217.2, E(): 0.00012, 28.1% identity in 217 aa overlap. Contains Pfam match to entry PF00589 Phage_integrase, Phage integrase family. Possible upstream and downstream alternative translational strat sites. 3 4 11 2 [Reporter]SCO2822 (20G13)_PCR/7276 [Gene]SCO2822/6628 NC_003888 SCBAC17F8.13c, possible decarboxylase, len: 243 aa: similar to many e.g. SW:Q05115 (AMDA_BORBR) arylmalonate decarboxylase from Alcaligenes bronchisepticus (240 aa) fasta scores; opt: 258, Z-score: 293.3, 33.333% identity (34.302% ungapped) in 177 aa overlap. Almost identical to TR:Q54412 (EMBL:X52071) hypothetical protein which close alongside transfer RNA-gly in Streptomyces lividans (195 aa) fasta scores; opt: 1233, Z-score: 1358.6, 98.980% identity (99.487% ungapped) in 196 aa overlap. Also similar to neighbouring CDS SCBAC17F8.14c. 3 4 10 22 [Reporter]SCO5268 (1G9)_PCR/7274 [Gene]SCO5268/6627 NC_003888 2SC7G11.30, unknown, len: 156 aa 3 4 10 21 [Reporter]SCO7010 (2C9)_PCR/7273 [Gene]SCO7010/6626 NC_003888 SC8F11.36c, aglA, alpha glucosidase, len: 534 aa. Identical to Streptomyces lividans TR:O86874(EMBL:U22894) alpha glucosidase, AglA (534 aa), fasta scores opt: 3764 z-score: 4189.4 E(): 0 99.6% identity in 534 aa overlap. Also highly similar to many other glycosyl hydrolases e.g. Escherichia coli SW:TREC_ECOLI(EMBL:U06195) trehalose-6-phosphate hydrolase (EC 3.2.1.93) (551 aa), fasta scores opt: 882 z-score: 982.3 E():0 36.1% identity in 523 aa overlap and Streptomyces coelicolor SC10B7.07 (577 aa), fasta scores opt: 1026 z-score: 1004.4 E():0 55.0% identity in 565 aa overlap. Contains a Pfam match to entry PF00128 alpha-amylase, Alpha amylase. 3 4 10 20 [Reporter]SCO1599 (3O5)_PCR/7272 [Gene]SCO1599/6625 NC_003888 SCI35.21c, rpmI, 50S ribosomal protein L35, len: 64 aa; similar to many e.g. RL35_BACSU 50S ribosomal protein L35 (65 aa), fasta scores; opt: 179 z-score: 355.6 E(): 1.5e-12, 45.9% identity in 61 aa overlap 3 4 10 19 [Reporter]SCO6616 (4K5)_PCR/7271 [Gene]SCO6616/6624 NC_003888 SC1F2.13c, putative secreted protein, len: 476 aa; similar to several proteins from M. tuberculosis e.g. TR:O05897 (EMBL:Z95121) hypothetical protein MTCY20B11.29 (462 aa), fasta scores; opt: 573 z-score: 448.5 E(): 9.7e-18, 32.3% identity in 400 aa overlap. Contains possible N-terminal signal sequence 3 4 10 17 [Reporter]SCO2033 (6C5)_PCR/7269 [Gene]SCO2033/6623 NC_003888 SC4G6.02, possible citrate lyase beta chain, len: 274 aa; similar to SW:CILB_KLEPN citrate lyase beta chain from Klebsiella pneumoniae (289 aa) fasta scores; opt: 293, z-score: 325.3, E(): 8.5e-11, (33.5% identity in 284 aa overlap). 3 4 10 16 [Reporter]SCO3600 (7O1)_PCR/7268 [Gene]SCO3600/6622 NC_003888 SC66T3.11, hypothetical protein, len: 167 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. The start codon is uncertain; two possible starts codons are present 12 and 15 codons upstream 2 3 16 12 [Reporter]SCO2288 (11F6)_PCR/4877 [Gene]SCO2288/4449 NC_003888 SCC75A.34c, unknown, len: 108 aa. Similar to Streptomyces coelicolor TR:CAB58302(EMBL:AL121854) hypothetical 11.8 KD protein (106 aa), fasta scores opt:99 z-score: 145.3 E():0.96 31.2% identity in 93 aa overlap. 3 4 10 15 [Reporter]SCO5560 (8K1)_PCR/7267 [Gene]SCO5560/6621 NC_003888 SC7A1.04, ddlA, D-alanine-D-alanine ligase, len: 389 aa; similar to many eg. SW:DDLA_SALTY D-alanine-D-alanine ligase from Salmonella typhimurium (363 aa) fasta scores; opt: 742, z-score: 1018.5, E(): 0, (39.3% identity in 377 aa overlap). Contains PS00843 D-alanine--D-alanine ligase signature 1. 2 3 16 10 [Reporter]SCO3036 (13N2)_PCR/4875 [Gene]SCO3036/4448 NC_003888 SCE34.17, hypothetical protein, len: 319 aa; similar to TR:P96866 (EMBL:Z92771) Mycobacterium tuberculosis hypothetical 35.3 kD protein, 331 aa; fasta scores: opt: 1151 z-score: 1253.3 E(): 0; 55.4% identity in 332 aa overlap. Contains Pfam match to entry PF01933 UPF0052, Uncharacterised protein family 3 4 10 14 [Reporter]SCO6601 (9G1)_PCR/7266 [Gene]SCO6601/6620 NC_003888 SC8A6.22, probable secreted sugar binding protein, l en: 438 aa; similar to e.g. S. coelicolor TR:P72397 (EMBL:Y 07706) putative maltose-binding pootein malE (423 aa), fast a scores; opt: 425 z-score: 307.7 E(): 6.7e-10, 28.9% ident ity in 439 aa overlap. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane l ipoprotein lipid attachment site 2 3 16 9 [Reporter]SCO4960 (14J2)_PCR/4874 [Gene]SCO4960/4447 NC_003888 2SCK31.20c, possible sigma factor, len: 168 aa; similar to SW:RPOE_STRCO (EMBL:L29636) Streptomyces coelicolor RNA polymerase sigma-E factor SigE, 176 aa; fasta scores: opt: 108 z-score: 140.1 E(): 2.9; 30.8% identity in 156 aa overlap 2 3 16 8 [Reporter]SCO4620 (15F2)_PCR/4873 [Gene]SCO4620/4446 NC_003888 SCD39.20c, traB1, possible sporulation-related protein, len: 681 aa; highly similar to TR:Q54679 (EMBL:U23762) Streptomyces phaeochromogenes essential for intermycelial plasmid transfer and pock formation, TraB, 678 aa; fasta scores: opt: 1845 z-score: 2002.9 E(): 0; 51.0% identity in 679 aa overlap and middle domain similar to middle domain of SW:SP3E_BACSU (EMBL:M17445) Bacillus subtilis stage III sporulation protein E, SpoIIIE, 787 aa; fasta scores: opt: 208 z-score: 224.4 E(): 4.9e-05; 26.7% identity in 344 aa overlap. Contains Pfam match to entry PF01580 FtsK_SpoIIIE, FtsK/SpoIIIE family. Also contains possible hydrophobic membrane spanning regions 2 3 16 7 [Reporter]SCO3581 (16B2)_PCR/4872 [Gene]SCO3581/4445 NC_003888 SCH66.02c, unknown, len: 154aa; similar to many eg. TR:O69656 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (154 aa) fasta scores; opt: 425, z-score: 453.4, E(): 6.2e-18, (46.1% identity in 154 aa overlap). 2 3 16 6 [Reporter]SCO0583 (16N22)_PCR/4871 [Gene]SCO0583/4444 NC_003888 SCF55.07, probable cytochrome P450, len: 407 aa; similar to SW:Y766_MYCTU (EMBL:Z80226) Mycobacterium tuberculosis putative cytochrome P450 RV0766c (EC 1.14.-.-) 402 aa ; fasta scores: opt: 601 z-score: 706.2 E(): 5.8e-32; 33.3% identity in 403 aa overlap, to SWC:PXI_BACME (EMBL:X16610) Bacillus megaterium cytochrome P450(BM-1) (EC 1.14.14.1) Cyp106, 410 aa; fasta scores: opt: 565 z-score: 664.0 E(): 1.3e-29; 30.6% identity in 405 aa overlap and to Streptomyces coelicolor SCF55.08c, 420 aa; fasta scores: opt: 204 z-score: 221.7 E(): 5.2e-07; 28.1% identity in 417 aa overlap. Contains match to Pfam entry PF00067 p450, Cytochrome P450 2 3 16 5 [Reporter]SCO1601 (17J22)_PCR/4870 [Gene]SCO1601/4443 NC_003888 SCI35.23, unknown, len: 121 aa 1 2 22 18 [Reporter]SCO7102 (5E19)_PCR/2495 [Gene]SCO7102/2269 NC_003888 SC4B10.03c, conserved hypothetical protein, len: 184 aa; similar to TR:CAB89770 (EMBL:AL354616) Streptomyces coelicolor hypothetical 19.1 kD protein SC3A4.24c, 178 aa; fasta scores: opt: 394 z-score: 403.2 E(): 5.4e-15; 55.5% identity in 182 aa overlap. Contains a coiled-coil region at approx. residues 18..45 2 3 16 4 [Reporter]SCO3931 (18F22)_PCR/4869 [Gene]SCO3931/4442 NC_003888 SCQ11.14, hypothetical protein, len: 99 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 1 2 22 17 [Reporter]SCO7559 (6A19)_PCR/2494 [Gene]SCO7559/2268 NC_003888 SC5F1.13c, probable secreted sugar hydrolase, len: 398 aa; similar to SW:E13B_ARTSP (EMBL:D23668) Arthrobacter sp. glucan endo-1,3-beta-glucosidase precursor (EC 3.2.1.39) GlcI, 548 aa; fasta scores: opt: 1570 z-score: 1670.2 E(): 0; 59.7% identity in 387 aa overlap and to Streptomyces coelicolor probable secreted hydrolase SC5F1.30c, 397 aa; fasta scores: opt: 1795 z-score: 1707.3 E(): 0; 66.6% identity in 386 aa overlap. Contains possible N-terminal signal peptide sequence 2 3 16 3 [Reporter]SCO7513 (19B22)_PCR/4868 [Gene]SCO7513/4441 NC_003888 SCBAC25F8.05c, possible secreted hydrolase, len: 295 aa; similar to TR:Q9S2A5 (EMBL:AL096849) Streptomyces coelicolor putative secreted hydrolase SCI11.14c, 268 aa; fasta scores: opt: 215 Z-score: 250.2 bits: 54.2 E(): 2.5e-06; 29.304% identity in 273 aa overlap. Contains Pfam match to entry PF00657 Lipase_GDSL, Lipase/Acylhydrolase with GDSL-like motif and possible N-terminal region signal peptide sequence 2 1 5 14 [Reporter]SCO5948 (8B24)_PCR/605 [Gene]SCO5948/549 NC_003888 SC7H1.18, possible oxidoreductase, len: 397 aa; weak similarity to TCMG_STRGA P39888 tetracenomycin polyketide synthesis hydroxylase (572 aa), fasta scores; opt: 72 z-score: 275.1 E(): 4e-08, 26.0% identity in 369 aa overlap. Also similar to M. tuberculosis hypothetical protein Y08M_MYCTU Q11058 MTCY50.22c (372 aa), fasta scores; opt: 949 z-score: 613.3 E(): 5.8e-27, 45.9% identity in 355 aa overlap 1 2 22 16 [Reporter]SCO1442 (7M15)_PCR/2493 [Gene]SCO1442/2267 NC_003888 SC6D7A.05c, possible integral membrane protein, len: 214 aa. Contains possible hydrophobic membrane spanning regions 2 3 15 22 [Reporter]SCO1207 (1J14)_PCR/4865 [Gene]SCO1207/4440 NC_003888 2SCG58.07, probable cytochrome P450, len: 404 aa; similar to SW:CPXF_STRGO (EMBL:M32239) Streptomyces griseolus cytochrome P450-Su2 (EC 1.14.-.-) SubC, 402 aa; fasta scores: opt: 791 z-score: 895.3 E(): 0; 37.1% identity in 412 aa overlap and to TR:CAB88975 (EMBL:AL353864) Streptomyces coelicolor cytochrome P450 SC8F11.24c, 407 aa; fasta scores: opt: 1736 z-score: 1959.5 E(): 0; 61.2% identity in 407 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450 and match to Prosite entry PS00086 Cytochrome P450 cysteine heme-iron ligand signature 2 1 5 13 [Reporter]SCO6457 (9N20)_PCR/604 [Gene]SCO6457/548 NC_003888 SC9B5.24, lacZ*, beta-galactosidase, len: 1307 aa; similar to many e.g. TR:O85167 (EMBL:AF047824) beta-galactosidase from Bacillus megaterium (1034 aa) fasta scores: opt: 1750, z-score: 2221.6, E(): 0, (37.4% identity in 1256 aa overlap). Note that this CDS is approx. 300 residues longer than the homologues due to an extra region around residues 600 to 900. Contains PS00719 Glycosyl hydrolases family 2 signature 1 and PS00608 Glycosyl hydrolases family 2 acid /base catalyst. Also contains Pfam match to entry PF00703 glycosyl_hydro7, Glycosyl hydrolases family 2. 1 2 22 15 [Reporter]SCO1998 (8I15)_PCR/2492 [Gene]SCO1998/2266 NC_003888 SC7H2.12c, rpsA, 30S ribosomal protein S1, len: 502aa; similar to many eg. SW:RS1_ECOLI rpsA, 30S ribosomal protein S1 from Escherichia coli (557 aa) fasta scores; opt: 1016, z-score: 1019.6, E(): 0, (39.8% identity in 452 aa overlap). Also similar to TR:O06147 (EMBL:Z95554) 30S ribosomal protein S1 from Mycobacterium tuberculosis (481 aa) fasta scores; opt: 2420, z-score: 2422.2, E(): 0, (79.1% identity in 492 aa overlap). Contains four Pfam matches to entry PF00575 S1, S1 RNA binding domain. Also contains possible coiled-coil region around 400-410aa. 2 1 5 12 [Reporter]SCO2743 (10J20)_PCR/603 [Gene]SCO2743/547 NC_003888 SCC57A.14, hypothetical protein, len: 161 aa; similar to TR:Q9FC57 (EMBL:AL391515) Streptomyces coelicolor conserved hypothetical protein SC4B10.30c, 156 aa; fasta scores: opt: 624 Z-score: 777.1 E(): 1.2e-35; 60.897% identity in 156 aa overlap 1 2 22 14 [Reporter]SCO7535 (9E15)_PCR/2491 [Gene]SCO7535/2265 NC_003888 SC8G12.11, possible lipoprotein, len: 187 aa. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 2 1 5 11 [Reporter]SCO4066 (11F20)_PCR/602 [Gene]SCO4066/546 NC_003888 SCD25.02, unknown, len: 357 aa. 1 2 22 13 [Reporter]SCO1939 (10A15)_PCR/2490 [Gene]SCO1939/2264 NC_003888 SCC22.21, probable 6-phosphogluconolactonase, len: 261 aa; similar to many e.g. SW:DEVB_MYCTU DevB protein homolog from Mycobacterium tuberculosis (247 aa) fasta scores; opt: 825, z-score: 921.3, E(): 0, (53.1% identity in 241 aa overlap) and SW:O95336 (6PGL_HUMAN) 6-phosphogluconolactonase from human (258 aa) fasta scores; opt: 392 Z-score: 437.3, E(): 1.3e-17, 36.250% identity (39.189% ungapped) in 240 aa overlap. Contains weak Pfam match to entry PF01182 Glucosamine_iso, Glucosamine-6-phosphate isomerase. 2 1 5 10 [Reporter]SCO4303 (12B20)_PCR/601 [Gene]SCO4303/545 NC_003888 SCD95A.36c, possible transcriptional regulator, len: 202 aa; similar to SW:YCFQ_ECOLI (EMBL:AE000211) Escherichia coli hypothetical transcriptional regulator in Ndh-Mfd intergenic region YcfQ, 210 aa; fasta scores: opt: 401 z-score: 492.0 E(): 6.1e-20; 37.3% identity in 201 aa overlap 1 2 22 12 [Reporter]SCO4341 (11M11)_PCR/2489 [Gene]SCO4341/2263 NC_003888 SCD12A.24, unknown, len: 104 aa 2 1 5 9 [Reporter]SCO0924 (13N16)_PCR/600 [Gene]SCO0924/544 NC_003888 SCM10.12c, possible cytochrome B subunit subunit, len: 243 aa; similar to SW:FRDC_HELPY (EMBL:U78101) Helicobacter pylori fumarate reductase cytochrome B subunit FrdC, 255aa; fasta scores: opt: 106 z-score: 139.5 E(): 2.2; 22.1% identity in 181 aa overlap 1 2 22 11 [Reporter]SCO4155 (12I11)_PCR/2488 [Gene]SCO4155/2262 NC_003888 SCD84.22c, probable two-component system sensor, len: 481 aa; similar to TR:O53895 (EMBL:AL021999) Mycobacterium tuberculosis putative two-component sensor, MTV044.10, 504 aa; fasta scores: opt: 884 z-score: 949.8 E(): 0; 39.4% identity in 482 aa overlap and to SW:AFQ2_STRCO (EMBL:D10654) Streptomyces coelicolor sensor protein AfsQ2 (EC 2.7.3.-), 535 aa; fasta scores: opt: 404 z-score: 435.9 E(): 8e-17; 31.4% identity in 526 aa overlap. Contains Pfam matches to entries PF00672 DUF5, Domain found in bacterial signal proteins and PF00512 signal, Histidine kinase and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains possible hydrophobic membrane spanning regions 2 1 5 8 [Reporter]SCO1689 (14J16)_PCR/599 [Gene]SCO1689/543 NC_003888 SCI30A.10, possible phosphotransferase, len: 341 aa; similar to TR:O69727 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (351 aa) fasta scores; opt: 872, z-score: 1004.6, E(): 0, (43.1% identity in 350 aa overlap). Also weakly similar to TR:Q53826 (EMBL:U13078) capreomycin phopshotransferase from Streptomyces capreolus (281 aa) fasta scores; opt: 142, z-score: 169.4, E(): 0.042, (29.1% identity in 172 aa overlap). 1 2 22 10 [Reporter]SCO3006 (13E11)_PCR/2487 [Gene]SCO3006/2261 NC_003888 SCE33.08, possible acetyltransferase, len: 191 aa; similar to TR:Q9Z576 (EMBL:AL035569) Streptomyces coelicolor putative acetyltransferase SC8D9.15, 194 aa; fasta scores: opt: 212 z-score: 283.0 E(): 2.6e-08; 40.5% identity in 84 aa overlap. Contains possible hydrophobic membrane spanning region 2 1 5 7 [Reporter]SCO2978 (15F16)_PCR/598 [Gene]SCO2978/542 NC_003888 SCE50.06, possible secreted protein, len: 465 aa; similar to TR:Q55471 (EMBL:D64006) Synechocystis sp. hypothetical 46.5 kD protein, 421 aa; fasta scores: opt: 788 z-score: 803.7 E(): 0; 35.2% identity in 386 aa overlap, to C-terminal part of SW:MSME_STRMU (EMBL:M77351) Streptococcus mutans multiple sugar binding protein precursor MsmE, 420 aa; fasta scores: opt: 240 z-score: 249.6 E(): 1.9e-06; 25.7% identity in 303 aa overlap and to SW:AGLE_RHIME (EMBL:AF045609) Rhizobium meliloti (Sinorhizobium meliloti) alpha-glucosidase-binding periplasmic protein AglE precursor, 429 aa; fasta scores: opt: 503 Z-score: 522.9 E(): 1.7e-21; 35.452% identity in 409 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide sequence 1 2 22 9 [Reporter]SCO0196 (14A11)_PCR/2486 [Gene]SCO0196/2260 NC_003888 SCJ12.08, unknown, len:134 aa. Shares a low level of similarity with Mycobacterium tuberculosis TR:O53597 (EMBL; AL021426) hypothetical 24.0 KD protein MTV028.10C (224 aa), fasta scores opt: 119 z-score: 159.2 E(): 0.1 32.8% identity in 116 aa overlap. 2 1 5 6 [Reporter]SCO1639 (16B16)_PCR/597 [Gene]SCO1639/541 NC_003888 SCI41.22c, probable secreted peptidyl-prolyl cis-trans isomerase protein, len: 335 aa. Similar to TR:Q53919 (EMBL:Z34523) Streptomyces chrysomallus FkbB, 312 aa; fasta scores: opt: 984 z-score: 1016.1 E():0; 49.1% identity in 336 aa overlap. Contains two Pfam matches to entry PF00254 FKBP, FKBP-type peptidyl-prolyl cis-trans isomerases and two Prosite matches to entry PS00454 FKBP-type peptidyl-prolyl cis-trans isomerase signature 2 and also PS00013 prokaryotic membrane lipoprotein lipid attachment site in the correct position. C-terminal contains similarities with Streptomyces coelicolor StI41.21c, (244 aa); fasta scores: opt: 134 z-score: 133.2 E():0.04; 31.1% identity in 177 aa overlap 2 1 5 5 [Reporter]SCO0597 (17N12)_PCR/596 [Gene]SCO0597/540 NC_003888 SCF55.21c, unknown, len: 131 aa; sequenced as TR:AAK61188 (EMBL:AF06826) Streptomyces coelicolor A3(2) ORFA, 131 aa 3 4 10 13 [Reporter]SCO6476 (10C1)_PCR/7265 [Gene]SCO6476/6619 NC_003888 SC9C7.12, unknown, len: 197 aa; contains TTA Leu codon, possible target for bldA regulation 3 4 10 12 [Reporter]SCO2756 (10O21)_PCR/7264 [Gene]SCO2756/6618 NC_003888 SCC57A.27c, integral membrane protein, len: 311 aa. Similar to many proteins of undefined function including: Deinococcus radiodurans TR:AAF09725(EMBL:AE001876) conserved hypothetical protein (325 aa), fasta scores opt: 713 z-score: 803.2 E(): 0 41.8% identity in 280 aa overlap and Bacillus subtilis SW:YOAV_BACSU(EMBL:AF027868) hypothetical 33.0 KD protein (292 aa), fasta scores opt: 355 z-score: 404.7 E(): 3.8e-15 26.3% identity in 297 aa overlap. Contains 2xPfam matches to entry PF00892 DUF6, Integral membrane protein. 3 4 10 10 [Reporter]SCO4316 (12G21)_PCR/7262 [Gene]SCO4316/6617 NC_003888 SCD95A.49c, possible ATP/GTP binding protein, len: 681 aa; similar to TR:CAB72415 (EMBL:AL138978) Streptomyces coelicolor hypothetical 82.6 kD protein SC6A11.15, 755 aa; fasta scores: opt: 683 z-score: 757.1 E(): 0; 33.5% identity in 767 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 4 10 8 [Reporter]SCO2953 (14O17)_PCR/7260 [Gene]SCO2953/6616 NC_003888 SCE59.12c, possible membrane protein, len: 262 aa; similar to TR:CAB63193 (EMBL:AL133469) Streptomyces coelicolor putative membrane protein SCM10.32, 216 aa; fasta scores: opt: 204 z-score: 218.0 E(): 9.9e-05; 37.2% identity in 253 aa overlap. Contains possible hydrophobic membrane spanning region 3 4 10 7 [Reporter]SCO2882 (15K17)_PCR/7259 [Gene]SCO2882/6615 NC_003888 SCE6.19, cvnD12, possible ATP/GTP-binding protein, len: 182 aa. Highly similar to many other ATP/GTP-binding proteins from Streptomyces coelicolor including: TR:CAB59480 (EMBL:AL132648) SCI41.10C (176 aa), fasta scores opt: 618 z-score: 748.9 E(): 0 58.4% identity in 166 aa overlap and TR:Q9X833 (EMBL:AL049727) SC9B1.13C (181 aa), fasta scores opt: 588 z-score: 712.9 E(): 2.8e-32 53.4% identity in 176 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop) 3 4 10 6 [Reporter]SCO0270 (16G17)_PCR/7258 [Gene]SCO0270/6614 NC_003888 SCF1.12, unknown, len: 466 aa; similar to proteins predicted to be involved in lantibiotic biosynthesis eg. SW:SPAC_BACSU subtilin biosynthesis protein from Bacillus subtilis (441 aa) fasta scores; opt: 502, z-score: 549.2, E(): 3.2e-23, (28.9% identity in 405 aa overlap) 3 4 10 5 [Reporter]SCO2989 (17C17)_PCR/7257 [Gene]SCO2989/6613 NC_003888 SCE50.17c, possible integral membrane protein, len: 145 aa; similar to TR:Q9X8C2 (EMBL:AL049763) Streptomyces coelicolor hypothetical 13.0 kD protein SCE36.09, 114 aa; fasta scores: opt: 256 z-score: 321.6 E(): 1.8e-10; 53.5% identity in 71 aa overlap. Contains possible N-terminal region signal peptide sequence and possible hydrophobic membrane spanning regions 3 4 10 4 [Reporter]SCP1.90c (18O13)_PCR/7256 [Gene]SCP1.90c/6612 NC_003888 SCP1.90c, possible ATP/GTP-binding protein, len: 228aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 2 3 15 21 [Reporter]SCO2988 (2F14)_PCR/4864 [Gene]SCO2988/4439 NC_003888 SCE50.16, galE2, UDP-glucose 4-epimerase, len: 326 aa; highly similar to SW:EXOB_AZOBR (EMBL:Z25478) Azospirillum brasilense UDP-glucose 4-epimerase (EC 5.1.3.2) ExoB, 348 aa; fasta scores: opt: 885 z-score: 970.7 E(): 0; 46.4% identity in 319 aa overlap and to SW:GALE_STRLI (EMBL:M18953) Streptomyces lividans UDP-glucose 4-epimerase (EC 5.1.3.2) GalE, 329 aa; fasta scores: opt: 793 z-score: 870.9 E(): 0;42.7% identity in 328 aa overlap. Contains Pfam match to entry PF01370 Epimerase, NAD dependent epimerase/dehydratase family 3 4 10 3 [Reporter]SCP1.317c (19K13)_PCR/7255 [Gene]SCP1.317c/6611 NC_003888 SCP1.317c, probable short-chain oxidoreductase, len: 331aa; previously sequenced as TR:Q9L8H0 (EMBL:AF204735). Also similar to many others eg. TR:Q9RR99 (EMBL:AE002088) oxidoreductase, short-chain dehydrogenase/reductase family from Deinococcus radiodurans (336 aa) fasta scores; opt: 795, z-score: 899.7, E(): 0, 44.7% identity in 320 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature. 2 3 15 20 [Reporter]SCO2218 (3B14)_PCR/4863 [Gene]SCO2218/4438 NC_003888 SC10B7.13c, possible lipoprotein, len: 148 aa; similar to TR:O07722 (EMBL:Z97193) Mycobacterium tuberculosis hypothetical 19.8 kD protein MTCY180.07, 201 aa; fasta scores: opt: 121 z-score: 149.5 E(): 0.6; 33.1% identity in 160 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 3 4 10 2 [Reporter]SCO3964 (20C13)_PCR/7254 [Gene]SCO3964/6610 NC_003888 SCBAC25E3.01c, possible integral membrane protein, len: >433aa: similar to TR:Q9AK64 (EMBL:AL583944) putative transport integral membrane protein 2SCD60.19 from Streptomyces coelicolor (589 aa) fasta scores; opt: 852, Z-score: 887.7, 51.646% identity (53.968% ungapped) in 395 aa overlap. Contains multiple possible membrane-spanning hydrophobic regions.,SCD78.31c, unknown, partial CDS len: >180 aa; contains two hydrophobic domains, 6 degenerate repeats of (G /T)KAS and 4 degenerate repeats of TAKGSG 2 3 15 19 [Reporter]SCO7012 (4N10)_PCR/4862 [Gene]SCO7012/4437 NC_003888 SC1H10.01c, possible binding protein dependent transport protein, partial CDS, len: > 288 aa. Almost identical to Streptomyces lividans TR:O86872(EMBL:U22894) integral membrane protein, AglF (231 aa), fasta scores opt: 1469 z-score: 1755.6 E(): 0 96.9% identity in 224 aa overlap. Also similar to Streptomyces coelicolor TR:CAB59596(EMBL:AL132662) probable sugar transporter inner membrane protein, SCF11.19 (316 aa), fasta scores opt: 575 z-score: 690.1 E(): 5.1e-31 38.1% identity in 273 aa overlap. Contains a Prosite hit to PS00402 Binding-protein-dependent transport systems inner membrane comp sign and a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Also contains multiple possible membrane spanning hydrophobic domains.,SC8F11.38c, possible membrane transport protein, partial CDS, len:> 71 aa. Similar to several e.g. Streptococcus mutans SW:MSMF_STRMU(EMBL:M77351) multiple sugar-binding transport system permease protein, MsmF (290 aa), fasta scores opt: 167 z-score: 244.0 E(): 3.8e-06 43.1% identity in 65 aa overlap. Contains possible membrane spanning hydrophobic domains. Overlaps and extends into CDS SC1H10.01c on the adjoining cosmid. 2 3 15 18 [Reporter]SCO2091 (5J10)_PCR/4861 [Gene]SCO2091/4436 NC_003888 SC4A10.24c, possible membrane protein, len: 206 aa; unknown function, previously partially sequenced as TR:Q9Z5V8 (EMBL:AF123319) Streptomyces coelicolor hypothetical protein (fragment) (163 aa) and identical to that sequence. Similar to a central region of mycobacterial hypothetical proteins e.g. TR:O06213 (EMBL:Z95388) Mycobacterium tuberculosis hypothetical protein (384 aa), fasta scores; opt: 282 z-score: 255.8 E(): 6.8e-07, 36.2% identity in 224 aa overlap. Contains a hydrophobic, possible membrane-spanning region and probable coiled-coil from 74 to 109 (36 residues) (Max score: 1.684, probability 1.00) 2 3 15 17 [Reporter]SCO5790 (6F10)_PCR/4860 [Gene]SCO5790/4435 NC_003888 SC4H2.11c, unknown, len: 95 aa 2 3 15 16 [Reporter]SCO5685 (7B10)_PCR/4859 [Gene]SCO5685/4434 NC_003888 SC5H4.09c, probable sugar hydrolase, len: 960 aa; similar to TR:Q59506 (EMBL:L06134) Microbispora bispora glucan-glucohydrolase (EC 3.2.1.74) (glucan 1,4-beta-glucosidase) (exo-1,4-beta-glucosidase) BglA, 986 aa; fasta scores: opt: 1257 z-score: 1346.1 E(): 0; 47.7% identity in 979 aa overlap. Contains Pfam matches to entries PF00933 Glyco_hydro_3, Glycosyl hydrolase family 3 N terminal domain and PF01915 Glyco_hydro_3_C, Glycosyl hydrolase family 3 C terminal domain 2 3 15 15 [Reporter]SCO2469 (8N6)_PCR/4858 [Gene]SCO2469/4433 NC_003888 SC7A8.08c, probable reductase, len: 421 aa; identical to previously sequenced TR:CAB51550 (EMBL:AJ244019) Streptomyces coelicolor A3(2) dnaG gene and flanking genes and similar to SW:CAMA_PSEPU (EMBL:J05406) Pseudomonas putida putidaredoxin reductase (EC 1.18.1.-) CamA, 422 aa; fasta scores: opt: 805 z-score: 922.5 E(): 0; 35.8% identity in 422 aa overlap 1 2 22 8 [Reporter]SCO5010 (15M7)_PCR/2485 [Gene]SCO5010/2259 NC_003888 SCK15.12, possible integral membrane protein, len: 316 aa; similar to TR:AAF40196 (EMBL:AF229646) Caulobacter crescentus hypothetical 34.6 kD protein, 325 aa; fasta scores: opt: 287 z-score: 328.2 E(): 8.2e-11; 21.9% identity in 315 aa overlap. Contains possible N-terminal region signal peptide sequence and possible hydrophobic membrane spanning regions 2 3 15 14 [Reporter]SCO7832 (9J6)_PCR/4857 [Gene]SCO7832/4432 NC_003888 SC8E7.29, possible Na+/H+ antiporter, len: 410 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1X8(EMBL:AL109973) putative Na+/H+ antiporter, SCJ30.11c (410 aa). Also highly similar to many other antiporters e.g. Haemophilus influenzae SW:NHAA_HAEIN(EMBL:U32708) Na(+)/H(+) antiporter 1 (400 aa), fasta scores opt: 504 z-score: 559.3 E(): 1.1e-23 36.6% identity in 391 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 1 2 22 7 [Reporter]SCO3866 (16I7)_PCR/2484 [Gene]SCO3866/2258 NC_003888 SCH18.03c, hypothetical protein, len: 291 aa; similar to TR:P74357 (EMBL:D90914) Synechocystis sp. hypothetical 43.7 kD protein, 368 aa; fasta scores: opt: 243 z-score: 281.6 E(): 3.3e-08; 29.1% identity in 206 aa overlap 2 3 15 13 [Reporter]SCO5708 (10F6)_PCR/4856 [Gene]SCO5708/4431 NC_003888 SC9F2.08c, possible ribosome-binding factor, len: 160aa; similar to many eg. SW:RBFA_ECOLI ribosome-binding factor, RbfA from Escherichia coli (132 aa) fasta scores; opt: 221, z-score: 268.0, E(): 1.3e-07, (36.1% identity in 119 aa overlap). 2 1 5 4 [Reporter]SCO1199 (18J12)_PCR/595 [Gene]SCO1199/539 NC_003888 SCG11A.30c, probable oxidoreductase, len: 326 aa; highly similar to TR:Q54856 (EMBL:X61931) Streptomyces purpurascens famA (FAS domain), 230 aa; fasta scores: opt: 1015 z-score: 1089.1 E(): 0; 79.5% identity in 239 aa overlap and to SW:QOR_PSEAE (EMBL:X85015) Pseudomonas aeruginosa quinone oxidoreductase (EC 1.6.5.5) (NADH:quinone reductase) Qor, 325 aa; fasta scores: opt: 446 z-score: 482.5 E(): 1.7e-19; 34.1% identity in 323 aa overlap. Contains match to Pfam entry PF00107 adh_zinc, Zinc-binding dehydrogenases and match to Prosite entry PS01162 Quinone oxidoreductase / zeta-crystallin signature 1 2 22 6 [Reporter]SCO0714 (17E7)_PCR/2483 [Gene]SCO0714/2257 NC_003888 SCF42.24, possible oxidoreductase, len: 295 aa. Identical to the previously sequenced Streptomyces coelicolor TR:Q9ZIW7 (EMBL:AF009336) Orf1 (fragment) (>195 aa). Also weakly similar to Sinorhizobium meliloti TR:O68965 (EMBL:AF059313) myo-inositol dehydrogenase (EC 1.1.1.18) (330 aa), fasta scores opt: 242 z-score: 275.5 E(): 5.9e-08 26.5% identity in 302 aa overlap. Contains a Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family 2 3 15 12 [Reporter]SCO2284 (11B6)_PCR/4855 [Gene]SCO2284/4430 NC_003888 SCC75A.30c, possible membrane transport protein, len: 428 aa. Similar to many members of the drug resistance translocase family including: Escherichia coli SW:BCR_ECOLI(EMBL:X63703) bicyclomycin resistance protein (sulfonamide resistance protein) (396 aa), fasta scores opt: 767 z-score: 825.5 E():0 33.9% identity in 395 aa overlap and Bacillus subtilis TR:P96709(EMBL:AB001488) bicyclomycin resistance protein YdgK (402 aa), fasta scores opt: 1073 z-score: 1151.7 E():0 42.3% identity in 397 aa overlap. Contains a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and a Prosite hit to PS00216 Sugar transport proteins signature 1. Also contains multiple possible membrane spanning hydrophobic domains. 2 1 5 3 [Reporter]SCP1.288c (19F12)_PCR/594 [Gene]SCP1.288c/538 NC_003888 SCP1.288c, possible ATP/GTP-binding protein, len: 892aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 2 22 4 [Reporter]SCO6003 (19M3)_PCR/2481 [Gene]SCO6003/2256 NC_003888 SCBAC1C11.06c, possible DNA-binding protein, len: 286 aa; similar to TR:CAC01466 (EMBL:AL391014) Streptomyces coelicolor putative DNA-binding protein 2SCG1.17, 286 aa; fasta scores: opt: 528 z-score: 639.9 E(): 4.2e-28; 37.4% identity in 281 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and possible helix-turn-helix motif at residues 27..48 (+4.04 SD) 2 1 5 2 [Reporter]SCO4976 (20N8)_PCR/593 [Gene]SCO4976/537 NC_003888 2SCK31.36c, conserved hypothetical protein, len: 242 aa; similar to TR:O53776 (EMBL:AL021942) Mycobacterium tuberculosis hypothetical 28.5 kDa protein MTV039.17, 252 aa; fasta scores: opt: 509 z-score: 573.9 E(): 2e-24; 42.3% identity in 241 aa overlap. Contains Pfam match to entry PF01927 DUF82, Protein of unknown function DUF82 1 2 22 3 [Reporter]SCO4049 (20I3)_PCR/2480 [Gene]SCO4049/2255 NC_003888 2SCD60.15, probable antibiotic hydrolase, len: 782 aa; similar to SW:PAC2_PSES3 (EMBL:M18278) Pseudomonas sp. penicillin acylase II precursor (EC 3.5.1.11) AcyII, 773 aa; fasta scores: opt: 701 z-score: 597.1 E(): 1.1e-25; 33.5% identity in 841 aa overlap. Contains 3x Pfam matches to entry PF01804 Penicil_amidase, Penicillin amidase. Also contains 11x degenerate repeat (S/P)(E/K)(A/T)A(L/P)- and 4x degenerate repeat TP(L/E) 2 1 4 22 [Reporter]SCO4200 (1N4)_PCR/591 [Gene]SCO4200/536 NC_003888 2SCD46.14, possible membrane protein, len: 113aa; similar to SW:Q11143 (Y476_MYCTU) hypothetical protein from Mycobacterium tuberculosis (87 aa) fasta scores; opt: 212, z-score: 268.0, E(): 1.9e-07, 41.4% identity in 87 aa overlap. Contains possible membrane-spanning hydrophobic regions. 1 2 21 22 [Reporter]SCO1088 (1A23)_PCR/2477 [Gene]SCO1088/2254 NC_003888 2SCG4.04c, possible oxidoreductase, len: 272 aa; similar to TR:Q9X4X1 (EMBL:AF119621) Pseudomonas abietaniphila hypothetical protein; short-chain dehydrogenase/reductase family (SDR) DitG, 244 aa; fasta scores: opt: 251 z-score: 280.0 E(): 4.1e-08; 28.7% identity in 254 aa overlap and to SW:FABG_VIBHA (EMBL:U39441) Vibrio harveyi 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) FabG, 244 aa; fasta scores: opt: 206 z-score: 231.9 E(): 2e-05; 25.1% identity in 239 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 2 1 4 21 [Reporter]SCO5958 (2J4)_PCR/590 [Gene]SCO5958/535 NC_003888 SC7H1.28c, cbiO, probable cobalt transport system ATP binding protein, len: 284 aa; similar to many eg. CBIO_SALTY Q05596 cobalt transport ATP-binding protein (271 aa), fasta scores; opt: 567 z-score: 685.3 E(): 5.6e-31, 38.8% identity in 242 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and Pfam match to entry ABC_tran PF00005, ABC transporters, score 203.07 1 2 21 20 [Reporter]SCO6427 (3I19)_PCR/2475 [Gene]SCO6427/2253 NC_003888 SC1A6.16, probable integral membrane transport protein, len: 431 aa; weak similarity to e.g. Mycobacterium smegmatis TR:O31137 (EMBL:O31137) tetracycline-resistance determinant tetV (419 aa), fasta scores; opt: 389 z-score: 243.8 E(): 1.9e-06, 27.0% identity in 404 aa overlap 2 1 4 19 [Reporter]SCO6909 (4B4)_PCR/588 [Gene]SCO6909/534 NC_003888 SC1B2.15, unknown, len: 127 aa. 1 2 21 19 [Reporter]SCO5615 (4E19)_PCR/2474 [Gene]SCO5615/2252 NC_003888 SC2E1.32, unknown prophage gene, len: 116 aa 2 1 4 18 [Reporter]SCO7089 (4N24)_PCR/587 [Gene]SCO7089/533 NC_003888 SC3A4.15c, probable two-component system sensor kinase, len: 397 aa. Highly similar to many including Escherichia coli SW:NARX_ECOLI (EMBL:X13360) nitrate/nitrite sensor protein NarX (598 aa), fasta scores opt: 211 z-score:0.0 E(): 0.0 28.82% identity in 229 aa overlap and Streptomyces coelicolor TR:CAB59719 (EMBL:AL132707) putative two-component sensor kinase, SCF51.21C (397 aa), fasta scores opt: 1075 z-score: 1237.2 E(): 0 48.0% identity in 400 aa overlap. Contains possible membrane spanning hydrophobic domains. 1 2 21 18 [Reporter]SCO6830 (5A19)_PCR/2473 [Gene]SCO6830/2251 NC_003888 SC4A9.07, possible ArsR-family transcriptional regulator, len: 117 aa. Highly similar to Streptomyces coelicolor TR:Q9X8X8 (EMBL:AL078610) putative transcriptional regulator SCH35.28C (122 aa), fasta scores opt: 561 z-score: 746.6 E():0 78.0% identity in 109 aa overlap and Mycobacterium tuberculosis TR:P71941 (EMBL:Z80225) putative transcriptional regulator (126 aa), fasta scores opt: 359 z-score: 482.6 E(): 1.8e-19 56.9% identity in 116 aa overlap. Also similar to several other Streptomyces coelicolor putative ArsR-family transcriptional regulators including: SC1A2.21 (119 aa), fasta scores opt: 491 z-score: 582.6 E(): 5.2e-27 64.7% identity in 116 aa overlap and SC1A2.17c (120 aa), fasta scores opt: 295 z-score: 356.1 E(): 2.1e-14 45.5% identity in 99 aa overlap. Contains a Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family. 2 1 4 17 [Reporter]SCO7074 (5J24)_PCR/586 [Gene]SCO7074/532 NC_003888 SC4G1.40, possible membrane protein, len: 58 aa; similar to C-terminal region of TR:P96360 (EMBL:Z92539) Mycobacterium tuberculosis hypothetical 31.4 kDa protein, MTCY10G2.08 aa; fasta scores: opt: 213 z-score: 335.7 E(): 3.1e-11; 65.3% identity in 49 aa overlap. Contains possible hydrophobic membrane spanning region 1 2 21 17 [Reporter]SCO2188 (6M15)_PCR/2472 [Gene]SCO2188/2250 NC_003888 SC5F7.13, possible peptidase (putative secreted protein), len: 282 aa; similar to TR:CAB38504 (EMBL:AL035636) putative serine protease from Streptomyces coelicolor (385 aa) fasta scores; opt: 873, z-score: 979.4, E(): 0, (49.5% identity in 281 aa overlap). Contains a weak Pfam match to entry PF00089 trypsin, Trypsin. Contains possible N-terminal region signal peptide sequence 2 1 4 16 [Reporter]SCO6735 (6F24)_PCR/585 [Gene]SCO6735/531 NC_003888 SC5F2A.18, unknown, len: 161 aa; some simlarity to SW:Y06D_BPT4 hypothetical protein from bacteriophage T4 (155 aa) fasta scores; opt: 135, z-score: 180.1, E(): 0.01, (20.9% identity in 158 aa overlap). 2 1 4 15 [Reporter]SCO1435 (7B24)_PCR/584 [Gene]SCO1435/530 NC_003888 SC6D7.04, possible integral membrane protein, len: 347 aa. Highly similar to many proteins of unknown function including: Mycobacterium tuberculosis SW:YI41_MYCTU (EMBL:Z83859) hypothetical 36.7 KD protein (345 aa), fasta scores opt: 884 z-score: 1036.1 E(): 0 43.8% identity in 349 aa overlap and Streptomyces coelicolor TR:CAB53285 (EMBL:AL109972) putative integral membrane protein SCJ9A.24C (339 aa), fasta scores: opt: 705 z-score: 827.6 E():0 36.1% identity in 327 aa overlap. Also similar to the adjoining CDS SC6D7.03 (443 aa), fasta scores: opt: 651 z-score: 666.6 E(): 8.9e-32 36.6% identity in 344 aa overlap. Contains a Pfam matches to entries PF01595 DUF21, Domain of unknown function and 2x Pfam match to entry PF00571 CBS, CBS domain. Also contains a possible N-terminal signal sequence and possible membrane spanning hydrophobic domains 3 4 9 22 [Reporter]SCO5264 (1C9)_PCR/7252 [Gene]SCO5264/6609 NC_003888 2SC7G11.26c, unknown, len: 360 aa. Contains possible coiled-coil region at approx. residues 157..212. High content in alanine, glutamic acid and arginine amino acid residues. Also highly hydrophilic 3 4 9 21 [Reporter]SCO3661 (2O5)_PCR/7251 [Gene]SCO3661/6608 NC_003888 clpB, SCH10.39c, probable clp-family ATP-binding subunit, partial CDS, len: >853 aa; similar to many e.g. SW:CLPB_ECOLI (EMBL:M29364) Escherichia coli ATP-dependent protease regulatory subunit (857 aa), fasta scores; opt: 3039 z-score: 2963.3 E(): 0, 56.5% identity in 844 aa overlap. Similar to TR:O69936 (EMBL:AL023861), SC3C8.27c, clpA, Streptomyces coelicolor probable clp protease ATP-binding subunit (842 aa) (47.5% identity in 854 aa overlap) and SCE94.24c (EMBL:AL049628) Streptomyces coelicolor probable clp protease ATP-binding subunit (841 aa) (48.5% identity in 845 aa overlap). Contains Pfam match to entry PF00495 clpA_B, Chaperonin clpA/B, score 696.80, E-value 1e-205, PS00870 Chaperonins clpA/B signature 1, PS00871 Chaperonins clpA/B signature 2 and two PS00017 ATP/GTP-binding site motif A (P-loop). Contains probable coiled-coil from 421 to 491 (71 residues) (Max score: 1.708, probability 1.00),SCH44.01c, clpB, ATP-dependent protease ATP-binding subunit (fragment) len: >44 aa; highly similar to N-terminal domain of TR:Q9Z6E4 (EMBL:AF076980) Streptomyces albus g. heat shock protein ClpB, 857 aa; fasta scores: opt: 233 z-score: 369.6 E(): 4e-13; 81.8% identity in 44 aa overlap 3 4 9 20 [Reporter]SCO2596 (3K5)_PCR/7250 [Gene]SCO2596/6607 NC_003888 SCC88.07c, rpmA, 50S ribosomal protein L27, len: 84 aa; identical to SW:RL27_STRGR (EMBL:D87916) Streptomyces griseus 50S ribosomal protein L27 RpmA, 85 aa. Contains Pfam match to entry PF01016 Ribosomal_L27, Ribosomal L27 proteinand match to Prosite entry PS00831 Ribosomal protein L27 signature 3 4 9 19 [Reporter]SCO6925 (4G5)_PCR/7249 [Gene]SCO6925/6606 NC_003888 SC1B2.31c, doubtful CDS (putative membrane protein), len: 70 aa. Contains a TTA leucine codon, possible target for bldA regulation. Contains possible hydrophobic membrane spanning region 3 4 9 18 [Reporter]SCO5664 (5C5)_PCR/7248 [Gene]SCO5664/6605 NC_003888 SC3A9.03c, probable integral membrane protein, len: 149 aa; has Pro-, Gly-rich N-terminus and two possible transmembrane domains in the C-terminus; some similarity to M. tuberculosis hypothetical protein TR:E1264549 (EMBL:AL022121) MTV025.037 (451 aa), fasta scores; opt: 183 z-score: 182.2 E(): 0.0066, 33.1% identity in 145 aa overlap. Also similar to upstream gene SC3A9.04c, fasta scores; E(): 1.5e-06, 41.0% identity in 134 aa overlap 3 4 9 17 [Reporter]SCO5772 (6O1)_PCR/7247 [Gene]SCO5772/6604 NC_003888 SC4H8.11c, unknown, len: 182 aa 3 4 9 16 [Reporter]SCO3596 (7K1)_PCR/7246 [Gene]SCO3596/6603 NC_003888 SC66T3.07, probable D-alanine:D-alanine dipeptidase, len: 202 aa; highly similar to e.g. vanXst (EMBL:AF039028) Streptomyces toyocaensis D-ala-D-ala dipeptidase (208 aa), fasta scores; opt: 1137 z-score: 1354.4 E(): 0, 77.6% identity in 201 aa overlap. Also similar to e.g. SW:VANX_ENTF (EMBL:U35369), vanXB, Enterococcus faecalis D-alanyl-D-alanine dipeptidase (202 aa) (63.9% identity in 202 aa overlap) 3 4 9 15 [Reporter]SCO2146 (8G1)_PCR/7245 [Gene]SCO2146/6602 NC_003888 SC6G10.19c, possible aminotransferase, len: 476 aa; similar to SW:CSDB_ECOLI (EMBL:AE000263) Escherichia coli selenocysteine lyase (EC 4.4.1.16) CsdB, 406 aa; fasta scores: opt: 565 Z-score: 628.7 E(): 2.3e-27; 33.862% identity in 378 aa overlap. Contains Pfam match to entry PF00266 aminotran_5, Aminotransferases class-V. Note: C-terminus overlaps with downstream gene. 2 3 15 11 [Reporter]SCO4121 (12N2)_PCR/4854 [Gene]SCO4121/4429 NC_003888 SCD72A.07c, possible integral membrane protein, len: 425aa; contains possible membrane-spanning hydrophobic regions. Weakly similar to many eg. SW:P39843 (BMR2_BACSU) multidrug resistance protein 2 from Bacillus subtilis (400 aa) fasta scores; opt: 309, z-score: 339.7, E(): 1.9e-11, 22.8% identity in 394 aa overlap. 3 4 9 14 [Reporter]SCO6597 (9C1)_PCR/7244 [Gene]SCO6597/6601 NC_003888 SC8A6.18, probable beta-glucosidase, len: 859 aa; si milar to many e.g. BGLS_AGRTU beta-glucosidase (EC 3.2.1.21 ) (818 aa), fasta scores; opt: 810 z-score: 1139.9 E(): 0, 37.2% identity in 862 aa overlap 2 3 15 10 [Reporter]SCO3031 (13J2)_PCR/4853 [Gene]SCO3031/4428 NC_003888 SCE34.12, hypothetical protein, len: 117 aa; similar to TR:AAD4708 (EMBL:AF164439) Mycobacterium smegmatis hypothetical 6.0 kD protein (fragment), 54 aa; fasta scores: opt: 184 z-score: 255.1 E(): 9.4e-07; 46.3% identity in 54 aa overlap 3 4 9 13 [Reporter]SCO1479 (9O21)_PCR/7243 [Gene]SCO1479/6600 NC_003888 SC9C5.03c, probable guanylate kinase, len: 197 aa; similar to SW:KGUA_ECOLI (EMBL:M84400) Escherichia coli guanylate kinase (EC 2.7.4.8) Gmk, 207 aa; fasta scores: opt: 512 z-score: 591.3 E(): 1.8e-25; 45.5% identity in 178 aa overlap. Contains Pfam match to entry PF00625 Guanylate_kin, Guanylate kinase and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00856 Guanylate kinase signature 2 3 15 9 [Reporter]SCO3423 (14F2)_PCR/4852 [Gene]SCO3423/4427 NC_003888 SCE9.30, possible regulator, len: 154 aa; similar to TR:Q53897 (EMBL:X60316), AbaA, Streptomyces coelicolor ORFA from AbaA regulatory locus for antibiotic production (192 aa), fasta scores; opt: 278 z-score: 352.7 E(): 2.5e-12, 39.9% identity in 148 aa overlap. Also similar to several S. coelicolor hypothetical proteins including the N-terminus of TR:O86807 (EMBL:AL031031), SC7C7.02c, possible regulatory protein (916 aa) (35.0% identity in 137 aa overlap). Contains TTA (leucine) codon, possible target for bldA regulation, near N-terminus 2 3 15 8 [Reporter]SCO1842 (15B2)_PCR/4851 [Gene]SCO1842/4426 NC_003888 SCI8.27c, unknown, len: 447 aa. Similar to another Streptomyces coelicolor protein of unknown function: TR:CAB46408 (EMBL: AL096743) hypothetical 49.9 KD protein (475 aa), fasta scores opt: 380 z-score: 402.6 E(): 4.8e-15 32.1% identity in 492 aa overlap. 2 3 15 7 [Reporter]SCO0938 (15N22)_PCR/4850 [Gene]SCO0938/4425 NC_003888 SCM10.26, probable amino acid transporter protein, len: 468 aa; similar to TR:O69844 (EMBL:AL023702) Streptomyces coelicolor putative cationic amino acid transporter SC1C3.02, 503 aa; fasta scores: opt: 829 z-score: 890.5 E(): 0; 45.9% identity in 490 aa overlap and to SW:CTR2_HUMAN (EMBL:D29990) Homo sapiens (Human) low-affinity cationic amino acid transporter-2 AtrC2, 658 aa; fasta scores: opt: 446 z-score: 480.4 E(): 2.3e-19; 32.8% identity in 430 aa overlap. Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease and match to Prosite entry PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site 2 3 15 6 [Reporter]SCO3730 (16J22)_PCR/4849 [Gene]SCO3730/4424 NC_003888 SCH22A.08, possible oxidoreductase, len: 285 aa; similar to TR:Q9X7T6 (EMBL:AL049863) Streptomyces coelicolor putative oxidoreductase SC5H1.28c, 305 aa; fasta scores: opt: 778 z-score: 848.8 E(): 0; 52.1% identity in 288 aa overlap 2 3 15 5 [Reporter]SCO1794 (17F22)_PCR/4848 [Gene]SCO1794/4423 NC_003888 SCI5.02, conserved hypothetical protein, len: 179 aa; similar to many eg. TR:O06235 (EMBL:Z95388) hypothetical protein from Mycobacterium tuberculosis (176 aa) fasta scores; opt: 581, z-score: 658.0, E(): 2.6e-29, (52.9% identity in 172 aa overlap) 1 2 21 16 [Reporter]SCO1410 (7I15)_PCR/2471 [Gene]SCO1410/2249 NC_003888 SC6D7.29, putative GntR-family transcriptional regulator, len: 231 aa. Similar to many other regulators e.g. Bacillus licheniformis SW:GNTR_BACLI(EMBL:D31631) gluconate operon transcriptional repressor (P28 protein) (243 aa), fasta scores opt: 183 z-score: 234.8 E(): 1.1e-05 31.2% identity in 138 aa overlap and Chelatobacter heintzii SW:NTRA_CHEHE(EMBL:U39411) NTA operon transcriptional regulator (210 aa), fasta scores opt: 222 z-score: 284.0 E(): 1.9e-08 31.8% identity in 195 aa overlap. Contains a PS00043 Bacterial regulatory proteins, gntR family signature and a Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. Contains a putative helix-turn-helix motif between residues 44..65 (+2.58 SD). 2 3 15 4 [Reporter]SCO3155 (18B22)_PCR/4847 [Gene]SCO3155/4422 NC_003888 SCE87.06, unknown, len: 179 aa. 1 2 21 15 [Reporter]SCO5231 (8E15)_PCR/2470 [Gene]SCO5231/2248 NC_003888 SC7E4.28c, probable gntR-family transcriptional regulator, len: 254 aa; similar to SW:HUTC_PSEPU (EMBL:M33922) Pseudomonas putida histidine utilization repressor HutC, 248 aa; fasta scores: opt: 355 z-score: 415.1 E(): 1.2e-15; 31.6% identity in 234 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and match to Prosite entry PS00043 Bacterial regulatory proteins, gntR family signature. Contains also a possible helix-turn-helix motif at residues 45..66 (+3.08 SD) 2 3 15 3 [Reporter]SCO1298 (19N18)_PCR/4846 [Gene]SCO1298/4421 NC_003888 SCBAC36F5.09c, conserved hypothetical protein, len: 183 aa; similar to TR:Q9KB42 (EMBL:AP001514) Bacillus halodurans BH2092 protein, 162 aa; fasta scores: opt: 361 Z-score: 424.4 bits: 85.0 E(): 5e-16; 37.410% identity in 139 aa overlap. Contains Pfam match to entry PF00581 Rhodanese, Rhodanese-like domain 2 1 4 14 [Reporter]SCO7181 (8N20)_PCR/583 [Gene]SCO7181/529 NC_003888 SC8A11.09, probable branched amino acid transport system ATP-binding protein, len: 261 aa; similar to SW:LIVG_ECOLI (EMBL:J05516) Escherichia coli high-affinity branched-chain amino acid transport ATP-binding protein LivG, 255 aa; fasta scores: opt: 586 z-score: 615.7 E(): 7.9e-27; 41.6% identity in 250 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 2 21 14 [Reporter]SCO7531 (9A15)_PCR/2469 [Gene]SCO7531/2247 NC_003888 SC8G12.07c, putative integral membrane protein, len: 418 aa. Similar in parts to many including: Mycobacterium leprae TR:Q9ZBE3(EMBL:AL035300) putative acyltransferase (696 aa), fasta scores opt: 294 z-score: 328.6 E(): 7.6e-11 39.5% identity in 443 aa overlap and Neisseria meningitidis TR:CAB85413(EMBL:AL162758) putative inner membrane trans-acylase protein (622 aa), fasta scores opt: 468 z-score: 522.8 E(): 1.2e-21 31.2% identity in 359 aa overlap. Contains a Pfam match to entry PF01757 DUF33, Domain of unknown function. Also contains multiple possible membrane spanning hydrophobic domains. 2 3 15 2 [Reporter]SCO5993 (20F18)_PCR/4845 [Gene]SCO5993/4420 NC_003888 SCBAC16H6.28, possible membrane protein, len: 270aa: weakly similar to TR:BAB53967 (EMBL:AP003012) MLR7525 protein from Rhizobium loti (250 aa) fasta scores; opt: 340, Z-score: 379.4, 28.400% identity (30.603% ungapped) in 250 aa overlap. Contains a possible membrane-spanning hydrophobic region 2 1 4 12 [Reporter]SCO2306 (10F20)_PCR/581 [Gene]SCO2306/528 NC_003888 SCC30.14, probable ABC transporter integral membrane protein, len: 238 aa; similar to TR:Q9XAC7 (EMBL:AL096839) Streptomyces coelicolor putative integral membrane transport protein SCC22.11, 269 aa; fasta scores: opt: 219 z-score: 253.7 E(): 1.1e-06; 30.1% identity in 239 aa overlap. Contains possible hydrophobic membrane spanning regions 1 2 21 13 [Reporter]SCO2580 (10M11)_PCR/2468 [Gene]SCO2580/2246 NC_003888 SCC123.18c, possible membrane protein, len: 55 aa. Contains a possible membrane spanning hydrophobic domain. 2 1 4 11 [Reporter]SCO4593 (11B20)_PCR/580 [Gene]SCO4593/527 NC_003888 SCD20.11c, conserved hypothetical protein, len: 284 aa; similar to SW:YTFG_ECOLI (EMBL:U14003) Escherichia coli hypothetical 29,7 kDa protein in RplI-CpdB intergenic region YtfG, 286 aa; fasta scores: opt: 801 z-score: 906.2 E(): 0; 47.1% identity in 280 aa overlap 1 2 21 12 [Reporter]SCO4336 (11I11)_PCR/2467 [Gene]SCO4336/2245 NC_003888 SCD12A.19, possible marR-family protein, len: 145 aa; similar to TR:CAB56677 (EMBL:AL121596) Streptomyces coelicolor marR-family protein SCF51A.25, 148 aa; fasta scores: opt: 227 z-score: 290.1 E(): 1.1e-08; 38.6% identity in 132 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 2 1 4 10 [Reporter]SCO2904 (12N16)_PCR/579 [Gene]SCO2904/526 NC_003888 SCE19A.04c, rph, probable ribonuclease PH, len: 245 aa; highly similar to many e.g. SW:RNPH_ECOLI (EMBL:X00781), rph, Escherichia coli ribonuclease PH (238 aa), fasta scores; opt: 916 z-score: 1116.8 E(): 0, 60.8% identity in 240 aa overlap. Contains Pfam match to entry PF01138 RNase_PH, 3' exoribonuclease family and PS01277 Ribonuclease PH signature 1 2 21 11 [Reporter]SCO4176 (12E11)_PCR/2466 [Gene]SCO4176/2244 NC_003888 SCD66.13, conserved hypothetical protein, len: 280 aa; similar to TR:O86676 (EMBL:AL031371) Streptomyces coelicolor hypothetical 30.7 kD protein SC4G2.03, 277 aa; fasta scores: opt: 378 z-score: 466.2 E(): 1.7e-18; 31.7% identity in 262 aa overlap 2 1 4 9 [Reporter]SCO3156 (13J16)_PCR/578 [Gene]SCO3156/525 NC_003888 SCE87.07, possible penicillin-binding protein (putative secreted protein), len: 541 aa. Similar to many including: Bacillus subtilis SW:PBPB_BACSU (EMBL:L09703) penicillin-binding protein 2B (PBP-2B) (716 aa), fasta scores opt: 221 z-score: 233.6 E(): 1.2e-05 26.8% identity in 336 aa overlap and Streptomyces coelicolor TR: CAB45623 (EMBL:AL079356) putative penicillin-binding protein SC6G9.32 (485 aa), fasta scores opt: 241 z-score: 257.0 E(): 6.1e-07 28.9% identity in 464 aa overlap. Also similar to the neighbouring CDS SCE87.08 (591 aa), fasta scores opt: 653 z-score: 627.3 E(): 1.3e-29 45.1% identity in 550 aa overlap. Contains a Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and a possible N-terminal signal sequence. 1 2 21 10 [Reporter]SCO3001 (13A11)_PCR/2465 [Gene]SCO3001/2243 NC_003888 SCE33.03c, hypothetical protein, len: 167 aa; similar to TR:O0587 (EMBL:Z95121) Mycobacterium tuberculosis hypothetical 17.8 kD protein MTCY20B11.06c, 169 aa; fasta scores: opt: 415 z-score: 473.1 E(): 6.8e-19; 49.1% identity in 171 aa overlap 2 1 4 8 [Reporter]SCO2958 (14F16)_PCR/577 [Gene]SCO2958/524 NC_003888 SCE59.17c, possible transcriptional regulator, len: 395 aa; similar to TR:P95217 (EMBL:Z86089) Mycobacterium tuberculosis hypothetical 40.7 kD protein MCTY0A4.04c, 381 aa; fasta scores: opt: 1224 z-score: 1335.3 E(): 0; 52.3% identity in 377 aa overlap and C-terminal region similar to SW:CUTR_STRCO (EMBL:X58793) Streptomyces coelicolor transcriptional regulatory protein CutR, 217 aa; fasta scores: opt: 130 z-score: 151.1 E(): 0.53; 30.3% identity in 132 aa overlap. Contains Pfam match to entry PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 1 2 21 9 [Reporter]SCO0519 (14M7)_PCR/2464 [Gene]SCO0519/2242 NC_003888 SCF6.15c, possible oxidoreductase, len: 245 aa. Similar to many e.g. Bacillus megaterium SW:DHG2_BACME (EMBL; D90044) glucose 1-dehydrogenase II (EC 1.1.1.47) (GLCDH-II) (261 aa), fasta scores opt: 402 z-score: 449.5 E(): 1.1e-17 35.1% identity in 228 aa overlap. Also similar to many other Streptomyces coelicolor putative oxidoreductases e.g. TR:CAB41284 (EMBL; AL049707) (260 aa), fasta scores opt: 395 z-score: 365.3 E(): 4.8e-15 37.2% identity in 242 aa overlap. Contains a Pfam match to entry PF00106 adh_short, short chain dehydrogenase and a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature. 2 1 4 7 [Reporter]SCO2997 (15B16)_PCR/576 [Gene]SCO2997/523 NC_003888 SCE99.04, possible transferase, len: 746 aa; similar to TR:Q9L053 (EMBL:AL163672) Streptomyces coelicolor putative glycosyl transferase SCE50.10, 736 aa; fasta scores: opt: 1826 z-score: 2036.2 E(): 0; 46.9% identity in 749 aa overlap 1 2 21 8 [Reporter]SCO0382 (15I7)_PCR/2463 [Gene]SCO0382/2241 NC_003888 SCF62.08, probable UDP-glucose/GDP-mannose family dehydrogenase (putative secreted protein), len: 441 aa. Highly similar to many e.g. Pseudomonas syringae (pv. phaseolicola) SW: ALGD_PSESH (EMBL; AF001555) GDP-mannose 6-dehydrogenase (EC 1.1.1.132) (438 aa), fasta scores opt: 1453 z-score: 1666.9 E(): 0 49.2% identity in 445 aa overlap. Contains a Pfam match to entry PF00984 UDPG_MGDP_dh, UDP-glucose/GDP-mannose dehydrogenase family. 2 1 4 6 [Reporter]SCO3068 (16N12)_PCR/575 [Gene]SCO3068/522 NC_003888 SCE25.09, sig15, putative RNA polymerase sigma factor, len: 304 aa; identical to previously sequenced TR:CAB55836 (EMBL:AJ249581) Streptomyces coelicolor A3(2) putative RNA polymerase sigma factor Sig15, 304 aa. Contains Pfam match to entry PF00140 sigma70, Sigma-70 factor and match to Prosite entry PS00715 Sigma-70 factors family signature 1. Contains also possible helix-turn-helix motif at residues 269..290 (+6.83 SD) 1 2 21 7 [Reporter]SCO0637 (16E7)_PCR/2462 [Gene]SCO0637/2240 NC_003888 SCF56.21, hypothetical protein, len: 151 aa; similar to TR:O33313 (EMBL:AL008967) Mycobacterium tuberculosis hypothetical 16.0 kD protein MTV002.36c, 150 aa; fasta scores: opt: 671 z-score: 800.1 E(): 0; 67.8% identity in 146 aa overlap 2 1 4 5 [Reporter]SCO0971 (17J12)_PCR/574 [Gene]SCO0971/521 NC_003888 SCM11.26, probable lysR-family trancsriptional regulator, len: 303 aa; similar to TR:Q9X9U9 (EMBL:Y18817) Streptomyces coelicolor transcriptional regulator of lysR family, 307 aa; fasta scores: opt: 502 z-score: 591.8 E(): 1.4e-25; 41.9% identity in 284 aa overlap and to SW:TFDT_ALCEU (EMBL:U16782) Alcaligenes eutrophus transcriptional regulatory protein TfdT, 228 aa; fasta scores: opt: 408 z-score: 484.4 E(): 1.3e-19; 42.6% identity in 204 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Contains a possible helix-turn-helix motif at residues 18..39 (+4.71 SD) 2 1 4 4 [Reporter]SCO3627 (18F12)_PCR/573 [Gene]SCO3627/520 NC_003888 SCH10.05c, hypothetical protein, len: 66 aa; unknown function, probable CDS suggested by positional base preference and GC frame analysis 2 3 14 22 [Reporter]SCO0990 (1F14)_PCR/4843 [Gene]SCO0990/4419 NC_003888 2SCG2.03c, possible integral membrane protein, len: 329 aa. Contains possible hydrophobic membrane spanning regions 2 3 14 21 [Reporter]SCO4661 (2B14)_PCR/4842 [Gene]SCO4661/4418 NC_003888 SCD840A.07, fusA, elongation factor G, len: 708 aa; similar to SW:EFG_ECOLI (EMBL:X00415) Escherichia coli elongation factor G (EF-G) FusA, 703 aa; fasta scores: opt: 2711 z-score: 2957.5 E(): 0; 59.9% identity in 708 aa overlap and to SW:EFG2_STRCO (EMBL:AL031013) Streptomyces coelicolor elongation factor G FusB, SC8A6.10, 686 aa; fasta scores: opt: 1279 z-score: 1330.4 E(): 0; 49.1% identity in 697 aa overlap. Contains Pfam match to entry PF00009 GTP_EFTU, Elongation factor Tu family and to entry PF00679 EFG_C, Elongation factor G C-terminus and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00301 GTP-binding elongation factors signature 2 3 14 20 [Reporter]SCO5909 (3N10)_PCR/4841 [Gene]SCO5909/4417 NC_003888 SC10A5.14, unknown, len: 68 aa 2 3 14 19 [Reporter]SCO6945 (4J10)_PCR/4840 [Gene]SCO6945/4416 NC_003888 SC1G8.17c, possible membrane protein, len: 69 aa. Contains a possible membrane spanning hydrophobic domain. 2 3 14 18 [Reporter]SCO5446 (5F10)_PCR/4839 [Gene]SCO5446/4415 NC_003888 SC3D11.03c, probable neutral zinc metalloprotease, len: 549aa; similar to many eg. SW:P05806 (NPRE_BACCE) bacillolysin precursor (EC 3.4.24.28) from Bacillus cereus (566 aa) fasta scores; opt: 607, z-score: 636.5, E(): 4.8e-28, 34.7% identity in 596 aa overlap. Also similar to the neighbouring CDS SC3D11.04c fasta scores; opt: 2442, z-score: 2158.9, E(): 0, 69.7% identity in 544 aa overlap and TR:CAB69762 (EMBL:AL137187) putative metalloproteinase from Streptomyces coelicolor fasta scores; opt: 1290, z-score: 1143.7, E(): 0, 52.7% identity in 560 aa overlap. Contains Pfam match to entry PF01447 Peptidase_M4, Thermolysin family peptidase and Prosite match to PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Contains possible hydrophobic membrane spanning region 2 3 14 17 [Reporter]SCO5786 (6B10)_PCR/4838 [Gene]SCO5786/4414 NC_003888 SC4H2.07c, possible secreted hydrolase, len: 400 aa; similar in N-terminus to many e.g GUNA_STRLI P27035 endoglucanase CelA precursor (459 aa), fasta scores; opt: 376 z-score: 257.5 E(): 4e-07, 37.7% identity in 175 aa overlap. Contains a very Thr- Pro-rich region at aa 143-170, a possible N-terminal signal sequence and an appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. C-terminal region has no apparent similarities. Also contains a TTA leucine codon; a potential target for action of bldA 2 3 14 16 [Reporter]SCO6755 (7N6)_PCR/4837 [Gene]SCO6755/4413 NC_003888 SC6A5.04, possible integral membrane transferase, len: 227aa; similar to many eg. TR:O67379 (EMBL:AE000737) glucose-1-phophate thymidylyltransferase from Aquifex aeolicus (428 aa) fasta scores; opt: 206, z-score: 257.3, E(): 5e-07, (28.1% identity in 199 aa overlap). Contains possible membrane spanning hydrophobic region. 1 2 21 6 [Reporter]SCO0097 (17A7)_PCR/2461 [Gene]SCO0097/2239 NC_003888 SCJ11.26c, possible integral membrane protein, len: 106 aa; contains possible membrane spanning hydrophobic regions. 2 3 14 15 [Reporter]SCO2463 (8J6)_PCR/4836 [Gene]SCO2463/4412 NC_003888 SC7A8.02, probable ABC transporter, len: 577 aa; similar to TR:Q9WYC3 (EMBL:AE001710) Thermotoga maritima ABC transporter, ATP-binding protein, 577 aa; fasta scores: opt: 1411 z-score: 1575.1 E(): 0; 39.9% identity in 569 aa overlap and to SW:YC73_MYCTU (EMBL:Z77137) Mycobacterium tuberculosis hypothetical ABC transporter ATP-binding protein RV1273c, 582 aa; fasta scores: opt: 1966 z-score: 2193.8 E(): 0; 51.7% identity in 574 aa overlap. Contains Pfam two matches to entry PF00664 ABC_membrane, ABC transporter transmembrane region and two matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 2 21 5 [Reporter]SCO1029 (18M3)_PCR/2460 [Gene]SCO1029/2238 NC_003888 SCG20A.09, unknown, len: 75 aa 2 3 14 14 [Reporter]SCO7733 (9F6)_PCR/4835 [Gene]SCO7733/4411 NC_003888 SC8D11.24, possible transcriptional regulatory protein, len: 739 aa. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix. Also contains a possible helix-turn-helix motif at residues 40..61 (+4.44 SD) 2 1 4 3 [Reporter]SCP1.78 (19B12)_PCR/572 [Gene]SCP1.78/519 NC_003888 SCP1.78, unknown, len: 242aa; contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 1 2 21 4 [Reporter]SCO1870 (19I3)_PCR/2459 [Gene]SCO1870/2237 NC_003888 SCI39.17c, unknown, len: 202 aa 2 3 14 13 [Reporter]SCO5712 (10B6)_PCR/4834 [Gene]SCO5712/4410 NC_003888 SC9F2.04, possible peptide transport ATP-binding protein, len: 359aa; similar to many eg. SW:APPF_BACSU oligopeptide transport ATP-binding protein from Bacillus subtilis (329 aa) fasta scores; opt: 798, z-score: 814.6, E(): 0, (41.2% identity in 330 aa overlap). Also similar to TR:O86575 (EMBL:AL031184) oligopeptide ABC transporter ATP-binding protein from Streptomyces coelicolor (496 aa) fasta scores; opt: 861, z-score: 875.6, E(): 0, (42.0% identity in 357 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also similar to the gene immediately upstream (SC9F2.03) suggesting a local duplication event fasta scores; opt: 722, z-score: 545.2, E(): 1.6e-25, (42.8% identity in 327 aa overlap). Also contains Pfam match to entry PF00005 ABC_tran, ABC transporter, score 212.90, E-value 4.7e-60. 2 1 4 2 [Reporter]SCO4957 (20J8)_PCR/571 [Gene]SCO4957/518 NC_003888 2SCK31.17c, putative membrane protein, len: 372 aa; similar to TR:Q9S2T3 (EMBL:AL096884) Streptomyces coelicolor hypothetical 40.5 kDa protein SC4G6.25, 376 aa; fasta scores: opt: 334 z-score: 393.9 E(): 2.1e-14 34.3% identity in 324 aa overlap. Contains possible hydrophobic membrane spanning region 1 2 21 3 [Reporter]SCP1.29 (20E3)_PCR/2458 [Gene]SCP1.29/2236 NC_003888 SCP1.29, unknown, len: 152aa; 2 1 3 22 [Reporter]SCO4196 (1J4)_PCR/569 [Gene]SCO4196/517 NC_003888 2SCD46.10c, possible organophosphate acid anhydrase, len: 404aa; similar to TR:Q50432 (EMBL:M91040) organophosphate acid anhydrase from Mycobacterium sp. (409 aa) fasta scores; opt: 762, z-score: 836.3, E(): 0, 44.8% identity in 402 aa overlap and TR:Q9S1C6 (EMBL:AB007293) organophosphorus insecticide hydrolase from Arthrobacter sp. (415 aa) fasta scores; opt: 705, z-score: 774.0, E(): 0, 42.6% identity in 408 aa overlap. 1 2 21 2 [Reporter]SCO5099 (20M23)_PCR/2457 [Gene]SCO5099/2235 NC_003888 SCBAC28G1.25, possible membrane protein, len: 376 aa. Contains possible hydrophobic membrane spanning regions 2 1 3 21 [Reporter]SCO0775 (2F4)_PCR/568 [Gene]SCO0775/516 NC_003888 3SCF60.07, conserved hypothetical protein, len: 149 aa; identical to TR:O85698 (EMBL:AF072709) Streptomyces lividans hypothetical 16.7 kDa protein, 149 aa and similar to SW:YF58_MYCTU (EMBL:Z74020) Mycobacterium tuberculosis hypothetical 16.4 kDa protein MTCY48.07c, 148 aa; fasta scores: opt: 695 z-score: 894.5 E(): 0; 69.1% identity in 149 aa overlap 1 2 20 22 [Reporter]SCO1349 (1M19)_PCR/2455 [Gene]SCO1349/2234 NC_003888 2SCG61.31c, hypothetical protein, len: 251aa; strongly similar to TR:Q9RCK7 (EMBL:AJ242827) hypothetical protein from Streptomyces tendae (266 aa) fasta scores; opt: 1417, z-score: 167,3.0 E(): 0, 84.3% identity in 249 aa overlap and TR:Q9RKV0 (EMBL:AL132824) hypothetical protein from Streptomyces coelicolor (304 aa) fasta scores; opt: 728, z-score: 862.7, E(): 0, 55.5% identity in 247 aa overlap 2 1 3 20 [Reporter]SCO7333 (3B4)_PCR/567 [Gene]SCO7333/515 NC_003888 SC4G10.12c, pep2B, unknown, len: 453 aa. Previously sequenced Streptomyces coelicolor TR:O70013(EMBL:AJ001206) hypothetical protein, Pep2 (453 aa). Highly similar to Streptomyces coelicolor TR:CAB72417(EMBL:AL138978) hypothetical 50.3 kd protein, Pep2A or SC6A11.17c (464 aa), fasta scores opt: 1629 z-score: 1819.8 E(): 0 55.8% identity in 462 aa overlap. 1 2 20 21 [Reporter]SCO3179 (2I19)_PCR/2454 [Gene]SCO3179/2233 NC_003888 SCE87.30c, moaB, probable molybdenum cofactor biosynthesis protein (putative secreted protein), len: 179 aa. Highly similar to many including: Mycobacterium tuberculosis TR:O53877 (EMBL:AL022004) putative molybdopterin biosynthesis Mog protein (160 aa), fasta scores opt: 617 z-score: 660.3 E(): 2.1e-29 60.0% identity in 160 aa overlap and the C-terminus of the Synechococcus sp MoaC, MoaB fusion protein SW:MOCB_SYNP7 (EMBL:X99625) molybdenum cofactor biosynthesis protein CB (319 aa), fasta scores opt: 401 z-score: 430.6 E(): 1.3e-16 41.5% identity in 171 aa overlap. Contains a Prosite hit to PS01078 Molybdenum cofactor biosynthesis proteins signature 1 and a possible N-terminal signal sequence. 2 1 3 19 [Reporter]SCO6151 (3N24)_PCR/566 [Gene]SCO6151/514 NC_003888 SC1A9.15, ogt, methylated-DNA-protein-cysteine methyltransferase len: 186 aa; similar to many eg. DAT1_BACSU (EMBL:X15659) methylated-DNA-protein-cysteine methyltransferase from Bacillus subtilis (165 aa) fasta scores; opt:357, z-score: 478.2, E(): 2.3e-19, (41.4% identity in 157 aa overlap). Contains Pfam match to entry PF01035 Methyltrans, 6-O-methylguanine DNA methyltransferase, score 170.70, E-value 2.5e-47. 1 2 20 20 [Reporter]SCO7657 (3E19)_PCR/2453 [Gene]SCO7657/2232 NC_003888 SC10F4.30, possible secreted protein, len: 585 aa. Contains possible N-terminal region signal peptide sequence 2 1 3 18 [Reporter]SCO7085 (4J24)_PCR/565 [Gene]SCO7085/513 NC_003888 SC3A4.11, doubtful CDS, len: 86 aa. 1 2 20 19 [Reporter]SCO5611 (4A19)_PCR/2452 [Gene]SCO5611/2231 NC_003888 SC2E1.28, possible prophage transcriptional regulator, len: 71 aa; similar to the N-terminus of many e.g. TR:Q37946 Salmonella typhimurium bacteriophage L repressor protein C2 (220 aa), fasta scores; opt: 127 z-score: 227.1 E():2e-05, 45.7% identity in 46 aa overlap. Contains probable helix-turn-helix motif at aa 21 to 42 (Score 1829, +5.42 SD) 2 1 3 17 [Reporter]SCO6561 (5F24)_PCR/564 [Gene]SCO6561/512 NC_003888 SC4B5.11c, respiratory chain oxidoreductase, len: 642 aa; similar to the C-terminal region of many proteins involved in electron transfer e.g. TR:P77908 (EMBL:U73807) FdhA formate dehydrogenase alpha subunit from Moorella thermoacetica (893 aa) fasta scores; opt: 1532, z-score: 1338.5, E(): 0, (43.1% identity in 619 aa overlap). Contains Pfam match to entry PF00384 molybdopterin, Prokaryotic molybdopterin oxidoreductases, score 95.80, E-value 6.2e-30. Considering the corresponding homolgies, this CDS may have previously been located directly downsteam of SC4B5.09c. 1 2 20 18 [Reporter]SCO7684 (5M15)_PCR/2451 [Gene]SCO7684/2230 NC_003888 SC4C2.19, conserved hypothetical protein, len: 383 aa; similar to TR:Q46024 (EMBL:U02634) Corynebacterium diphtheriae DtxR/iron regulated protein 2 Irp2, 369 aa; fasta scores: opt: 241 z-score: 271.1 E(): 1.5e-07; 29.6% identity in 382 aa overlap 2 1 3 16 [Reporter]SCO6731 (6B24)_PCR/563 [Gene]SCO6731/511 NC_003888 SC5F2A.14, probable thiolase, len: 404aa; similar to many egs. TR:O53871 (EMBL:AL022004) putative beta-ketoadypyl coA tiolase from Mycobacterium tuberculosis (403 aa) fasta scores; opt: 1842, z-score: 1989.2, E(): 0, (67.6% identity in 404 aa overlap) and TR:O53017 (EMBL:X97452) acetyl coA thiolase from Escherichia coli (401 aa) fasta scores; opt: 819, z-score: 887.9, E(): 0, (41.7% identity in 424 aa overlap). Also similar to SC6A5.37 (EMBL:AL049485) probable acetyl coA acetyltransferase from Streptomyces coelicolor (404 aa) fasta scores; opt: 2480, z-score: 2352.6, E(): 0, (95.0% identity in 404 aa overlap). Contains Pfam match to entry PF00108 thiolase, Thiolase and Prosite matches to PS00737 Thiolases signature 2 and PS00099 Thiolases active site. 2 1 3 15 [Reporter]SCO6949 (7N20)_PCR/562 [Gene]SCO6949/510 NC_003888 SC6F7.02c, unknown, len: 153 aa. Weakly similar to several including: Streptomyces albus TR:AAD46514 (EMBL:AF145724) putative regulatory protein, SitI (137 aa), fasta scores opt:174 z-score: 238.9 E(): 7.2e-06 34.5% identity in 116 aa overlap. 2 3 14 12 [Reporter]SCO2358 (11N2)_PCR/4833 [Gene]SCO2358/4409 NC_003888 SCC8A.16c, possible two-component system response regulator, len: 212aa; similar to many proposed regulators eg. TR:CAB81859 (EMBL:AL161691) putative two-component system response regulator from Streptomyces coelicolor (210 aa) fasta scores; opt: 736, z-score: 875.7, E(): 0, 61.0% identity in 210 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain, Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and helix-turn-helix motif 164-185aa. 2 3 14 10 [Reporter]SCO3184 (13F2)_PCR/4831 [Gene]SCO3184/4408 NC_003888 SCE22.01c, possible secreted penicillin acylase (fragment), len: >162 aa; similar to C-terminal region of SW:PAC2_PSES3 (EMBL:M18278) Pseudomonas sp. penicillin acylase II precursor (EC 3.5.1.11) AcyII, 773 aa; fasta scores: opt: 291 z-score: 347.5 E(): 6.8e-12; 39.1% identity in 110 aa overlap. Contains possible N-terminal signal peptide sequence,SCE87.35c, possible penicillin acylase (EC 3.5.1.11) partial CDS, len: > 808 aa. Similar to many penicillin acylases including: Pseudomonas sp. (strain SE83) SW:PAC2_PSES3c (EMBL:M18278) penicillin acylase II precursor (EC 3.5.1.11) (penicillin amidase II) (773 aa), fasta scores opt: 308 z-score: 319.7 E(): 2e-10 27.2% identity in 815 aa overlap. Contains a Pfam match to entry PF01804 Penicil_amidase and a 12x 'TG' repeat region between residues 148..172. 2 3 14 9 [Reporter]SCO5053 (14B2)_PCR/4830 [Gene]SCO5053/4407 NC_003888 SCK7.26, possible secreted protein, len: 519 aa. Contains possible N-terminal region signal peptide sequence 2 3 14 8 [Reporter]SCO0839 (14N22)_PCR/4829 [Gene]SCO0839/4406 NC_003888 SCF43A.29c, probable transmembrane transport protein, len: 705 aa; similar to TR:Q53902 (EMBL:M64683) S.coelicolor actII ORF3 antibiotic export protein (711 aa), fasta scores; opt: 887 z-score: 912.2 E(): 0, 45.3% identity in 700 aa overlap and to many putative transporters and hypothetical proteins. Also similar to TR:O54101 (EMBL:AL021529) S.coelicolor probable export protein (847 aa) (37.2% identity in 702 aa overlap). Contains hydrophobic, possible membrane-spanning regions 2 3 14 7 [Reporter]SCO3846 (15J22)_PCR/4828 [Gene]SCO3846/4405 NC_003888 SCH69.16, probable FtsW/RodA/SpoVE family cell cycle protein, len: 479aa; similar to many egs. SW:FTSW_MYCTU probable cell division protein FtsW from Mycobacterium tuberculosis (469 aa) fasta scores; opt: 1367, z-score: 1568.6, E(): 0, (48.8% identity in 451 aa overlap) and SW:SP5E_BACSU stage V sporulation protein E from Bacillus subtilis (366 aa) fasta scores; opt: 496, z-score: 572.9, E(): 1.4e-24, (31.7% identity in 391 aa overlap). Also similar to SC6G9.31 from Streptomyces coelicolor (446 aa) fasta scores; opt: 1132, z-score: 1182.0, E(): 0, (58.6% identity in 444 aa overlap). Contains Pfam match to entry PF01098 FTSW_RODA_SPOVE, Cell cycle protein. Also contains possible membrane spanning hydrophobic regions. 2 3 14 6 [Reporter]SCO3769 (16F22)_PCR/4827 [Gene]SCO3769/4404 NC_003888 SCH63.16c, possible tetR family transcriptional regulatory protein, len: 245 aa; similar to TR:Q9WX19 (EMBL:AL079345) Streptomyces coelicolor putative transcriptional regulator SCE68.13, 230 aa; fasta scores: opt: 359 z-score: 384.4 E(): 6.8e-14; 37.2% identity in 231 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a possible helix-turn-helix motif at residues 38..59 (+2.58 SD) 2 3 14 5 [Reporter]SCO0751 (17B22)_PCR/4826 [Gene]SCO0751/4403 NC_003888 SCF81.10c, possible regulatory protein, len: 894 aa; similar to TR:Q9ZBJ0 (EMBL:AL035161) Streptomyces coelicolor conserved hypothetical protein SC9C7.20, 860 aa; fasta scores: opt: 663 z-score: 716.6 E(): 1.5e-32; 29.6% identity in 873 aa overlap and to TR:CAB44394 (EMBL:AL078610) Streptomyces coelicolor putative regulatory protein SCH35.33c, 422 aa; fasta scores: opt: 305 z-score: 312.4 E(): 5e-10; 33.3% identity in 258 aa overlap. Contains match to Pfam entry PF01590 GAF, GAF domain 1 2 20 17 [Reporter]SCO2192 (6I15)_PCR/2450 [Gene]SCO2192/2229 NC_003888 SC5F7.09c, possible regulator, len: 487 aa; similar to others from Streptomyces spp. e.g. TR:Q53958 (EMBL:M80614) sporulation associated proteins from Streptomyces coelicolor (473 aa) fasta scores; opt: 728, z-score: 812.2, E(): 0, (39.7% identity in 489 aa overlap) and TR:O88053 (EMBL:AL031541) putative transcriptional regulator from Streptomyces coelicolor (446 aa) fasta scores; opt: 501, z-score: 560.4, E(): 7e-24, (33.8% identity in 459 aa overlap). 2 3 14 4 [Reporter]SCO6281 (18N18)_PCR/4825 [Gene]SCO6281/4402 NC_003888 SC1G7.07, probable FAD-binding protein, len: 545 aa; similar to TR:AAG23271 (EMBL:AY007564) Saccharopolyspora spinosa putative oxidoreductase SpnJ, 539 aa; fasta scores: opt: 1599 Z-score: 1759.4 bits: 335.3 E(): 2.1e-90; 45.785% identity in 522 aa overlap and to TR:Q9EX55 (EMBL:AL512902) Streptomyces coelicolor putative secreted FAD-binding protein SC2C4.02, 550 aa; fasta scores: opt: 2105 Z-score: 2315.4 bits: 438.2 E(): 2.2e-121; 56.884% identity in 552 aa overlap. Contains Pfam match to entry PF01565 FAD_binding_4, FAD binding domain 1 2 20 16 [Reporter]SCO6170 (7E15)_PCR/2449 [Gene]SCO6170/2228 NC_003888 SC6C5.06, probable oxidoreductase subunit, len: 296aa; similar to many, both prokaryotic and eukaryotic egs. TR:Q59127 (EMBL:X75338) nicotine dehydrogenase from Arthrobacter nicotinovorans (283 aa) fasta scores; opt: 308, z-score: 490.8, E(): 4.8e-20, (29.9% identity in 281 aa overlap) and SW:XDH_DROPS xanthine dehydrogenase from Drosophila pseudoobscura (1342 aa) fasta scores; opt: 146, z-score: 404.4, E(): 3.1e-15, (31.3% identity in 240 aa overlap). Contains Pfam match to entry PF00941 dehydrog_molyb, Molybdopterin binding domain in dehydrogenase, score 41.00, E-value 1.2e-12. 2 3 14 3 [Reporter]SCO4030 (19J18)_PCR/4824 [Gene]SCO4030/4401 NC_003888 2SC10A7.34c, hypothetical protein, len: 273 aa; similar to TR:Q9RDM0 (EMBL:AL136518) Streptomyces coelicolor hypothetical 31.6 kDa protein SCC123.04c, 293 aa; fasta scores: opt: 731 z-score: 837.9 E(): 0; 45.8% identity in 264 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 1 3 14 [Reporter]SCO7177 (8J20)_PCR/561 [Gene]SCO7177/509 NC_003888 SC8A11.05, unknown, len: 214 aa 1 2 20 15 [Reporter]SCO5224 (8A15)_PCR/2448 [Gene]SCO5224/2227 NC_003888 SC7E4.21c, probable araC-family transcriptional regulator, len: 322 aa; similar to TR:Q9S166 (EMBL:AB023785) Streptomyces griseus transcriptional activator for strR, AdpA, 405 aa; fasta scores: opt: 1005 z-score: 1177.5 E(): 0; 49.2% identity in 325 aa overlapand to TR:CAB87229 (EMBL:AL163641) Streptomyces coelicolor araC-family transcriptional regulator AdpA, 398 aa; fasta scores: opt: 990 z-score: 1160.1 E(): 0; 48.9% identity in 313 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family and match to Prosite entry PS00041 Bacterial regulatory proteins, araC family signature. Contains also a possible helix-turn-helix motif at residues 230..251 (+3.72 SD) 2 3 14 2 [Reporter]SCO5981 (20B18)_PCR/4823 [Gene]SCO5981/4400 NC_003888 SCBAC16H6.16c, unknown, len: 303 aa: contains weak Pfam match to entry PF00685 Sulfotransfer, Sulfotransferase proteins 2 1 3 13 [Reporter]SCO7164 (9F20)_PCR/560 [Gene]SCO7164/508 NC_003888 SC9A4.26c, unknown, len: 672 aa 1 2 20 14 [Reporter]SCO5398 (9M11)_PCR/2447 [Gene]SCO5398/2226 NC_003888 SC8F4.02c, conserved hypothetical protein, len: 146aa; similar to several of undefined function eg. TR:O28065 (EMBL:AE000952) conserved hypothetical protein from Archaeoglobus fulgidus (131 aa) fasta scores; opt: 346 z-score: 449.3 E(): 1.3e-17 40.4% identity in 136 aa overlap. 2 1 3 12 [Reporter]SCO2302 (10B20)_PCR/559 [Gene]SCO2302/507 NC_003888 SCC30.10, unknown, len: 422 aa. High content in glycine, alanine and proline amino acid residues 1 2 20 13 [Reporter]SCO2576 (10I11)_PCR/2446 [Gene]SCO2576/2225 NC_003888 SCC123.14c, pgm2, phosphoglycerate mutase, len: 223 aa. Highly similar to several other putative phosphoglycerate mutases including: Escherichia coli SW:PMG2_ECOLI (EMBL:M97495) GpmB (215 aa), fasta scores opt: 252 z-score: 313.8 E(): 4.4e-10 32.5% identity in 194 aa overlap and Deinococcus radiodurans TR:AAF10667(EMBL:AE001959) (232 aa), fasta scores opt: 317 z-score: 392.0 E(): 1.9e-14 41.5% identity in 193 aa overlap. Contains a Prosite hit to PS00175 Phosphoglycerate mutase family phosphohistidine signature and a Pfam match to entry PF00300 PGAM, Phosphoglycerate mutase family. 2 1 3 11 [Reporter]SCO4518 (11N16)_PCR/558 [Gene]SCO4518/506 NC_003888 SCD35.25c, possible integral membrane protein, len: 449 aa. Contains possible hydrophobic membrane spanning regions and possible colied-coil region at aprox. residues 82..110 1 2 20 12 [Reporter]SCO2594 (11E11)_PCR/2445 [Gene]SCO2594/2224 NC_003888 SCC88.05c, hypothetical protein, len: 586 aa; similar to various hypothetical proteins, e.g. TR:O69853 (EMBL:AL023702) Streptomyces coelicolor hypothetical 66.0 kD protein SC1C3.11, 586 aa; fasta scores: opt: 1251 z-score: 1446.9 E(): 0; 46.5% identity in 600 aa overlap 2 1 3 10 [Reporter]SCO3302 (12J16)_PCR/557 [Gene]SCO3302/505 NC_003888 SCE15.19, possible integral membrane protein, len: 433aa; contains possible membrane spanning hydrophobic regions. 1 2 20 11 [Reporter]SCO4172 (12A11)_PCR/2444 [Gene]SCO4172/2223 NC_003888 SCD66.09, possible membrane protein, len: 291 aa. Contains possible hydrophobic membrane spanning region and high content in alanine amino acid residues 2 1 3 9 [Reporter]SCO1640 (13F16)_PCR/556 [Gene]SCO1640/504 NC_003888 SCI41.23c, unknown, len: 453 aa. Highly similar to Mycobacterium tuberculosis SW:YY37_MYCTU (EMBL:Z73966) hypothetical 51.4 kd protein CY49.37c, 452 aa; fasta scores: opt: 2309 z-score: 2768.8 E():0; 73.5% identity in 453 aa overlap. Contains also similarities with Streptomyces coelicolor StI41.30c, (497 aa); fasta scores: opt: 962 z-score: 1024.1 E():0; 41.9% identity in 413 aa overlap. 1 2 20 10 [Reporter]SCO3282 (13M7)_PCR/2443 [Gene]SCO3282/2222 NC_003888 SCE39.32, possible precorrin-8X methylmutase, len: 218 aa; similar to many e.g. SW:COBH_PSEDE Precorrin-8X methylmutase from Pseudomonas denitrificans (210 aa) fasta scores; opt: 868, z-score: 1000.1, E(): 0, (64.2% identity in 204 aa overlap) and SW:COBH_MYCTU precorrin-8X methylmutase from Mycobacterium tuberculosis (208 aa) fasta scores; opt: 852, z-score: 982.0, E(): 0, (64.1% identity in 206 aa overlap). 2 1 3 8 [Reporter]SCO0002 (14B16)_PCR/555 [Gene]SCO0002/503 NC_003888 SCEND.01c, unknown, partial CDS, len: >27aa; strongly similar to TR:AAF36550 (EMBL:AF194023) helicase-like protein from Streptomyces lividans (881 aa) fasta scores; opt: 114, z-score: 206.0, E(): 0.00052, 80.0% identity in 25 aa overlap.,SCJ24.01c, unknown, partial CDS, len: >457aa; strongly similar to TR:AAF36550 (EMBL:AF194023) helicase-like protein from Streptomyces lividans (881 aa) fasta scores; opt: 2191, z-score: 2490.1, E(): 0, 72.5% identity in 458 aa overlap.,SC8E7.42c, partial CDS, unknown, len:> 431 aa. Highly similar to Streptomyces lividans TR:AAF36550(EMBL:AF194023) helicase-like protein found in the chromosome terminal region (881 aa), fasta scores opt: 1940 z-score: 2170.3 E():0 66.9% identity in 438 aa overlap. 1 4 18 9 [Reporter]SCO0002 (14A5)_PCR/6429 [Gene]SCO0002/503 NC_003888 SCEND.01c, unknown, partial CDS, len: >27aa; strongly similar to TR:AAF36550 (EMBL:AF194023) helicase-like protein from Streptomyces lividans (881 aa) fasta scores; opt: 114, z-score: 206.0, E(): 0.00052, 80.0% identity in 25 aa overlap.,SCJ24.01c, unknown, partial CDS, len: >457aa; strongly similar to TR:AAF36550 (EMBL:AF194023) helicase-like protein from Streptomyces lividans (881 aa) fasta scores; opt: 2191, z-score: 2490.1, E(): 0, 72.5% identity in 458 aa overlap.,SC8E7.42c, partial CDS, unknown, len:> 431 aa. Highly similar to Streptomyces lividans TR:AAF36550(EMBL:AF194023) helicase-like protein found in the chromosome terminal region (881 aa), fasta scores opt: 1940 z-score: 2170.3 E():0 66.9% identity in 438 aa overlap. 1 2 20 9 [Reporter]SCO1470 (14I7)_PCR/2442 [Gene]SCO1470/2221 NC_003888 SCL6.27, hypothetical protein, len: 123 aa; similar to TR:Q53804 (EMBL:X73563) Streptomyces bambergiensis plasmid genes tra1 - tra5 and ORF4 and ORF7. Plasmid pBL1, 109 aa; fasta scores: opt: 134 z-score: 180.1 E(): 0.013; 31.1% identity in 74 aa overlap 2 1 3 7 [Reporter]SCO0885 (15N12)_PCR/554 [Gene]SCO0885/502 NC_003888 SCM1.18, trxA3, thioredoxin, len: 134 aa. Highly similar to many thioredoxins e.g. Escherichia coli SW:THI2_ECOLI (EMBL:U85942) thioredoxin 2 (Trx2) (139 aa), fasta scores opt: 280 z-score: 337.5 E(): 1.9e-11 41.6% identity in 101 aa overlap. Also similar to Streptomyces coelicolor TR:CAB42711 (EMBL:AL049826) thioredoxin TrxA, SCH24.11C (110 aa), fasta scores opt: 224 z-score: 263.9 E(): 2.5e-09 38.7% identity in 93 aa overlap. Contains a Prosite hit to PS00194 Thioredoxin family active site and a Pfam match to entry PF00085 thiored, Thioredoxin. 1 2 20 8 [Reporter]SCO0421 (15E7)_PCR/2441 [Gene]SCO0421/2220 NC_003888 SCF51.20c, possible two-component response regulator, len: 219 aa; similar to TR:CAB51249 (EMBL:AL096872) Streptomyces coelicolor putative two-component response regulator, SC5F7.36c, 227 aa; fasta scores: opt: 815 z-score: 952.9 E(): 0; 61.8% identity in 220 aa overlap and to TR:Q53894 (EMBL:U51332) Streptomyces coelicolor absA2, 222 aa; fasta scores: opt: 543 z-score: 638.7 E(): 3.3e-28; 43.8% identity in 217 aa overlap. Contains two Pfam matches to entries PF00196 GerE, Bacterial regulatory proteins, luxR family and PF00072 response_reg, Response regulator receiver domain and Prosite match to entry PS00017 ATP/GTP-binding site motif A (P-loop). Contains possible helix-turn-helix motif between residues 14157..14222, score 1198 (+3.27 SD) 2 1 3 5 [Reporter]SCO1650 (17F12)_PCR/552 [Gene]SCO1650/501 NC_003888 SCI41.33c, putative secreted protein, len: 196 aa. Identical to TR:087592 (EMBL:AF086832) Streptomyces coelicolor hypothetical 20.2 Kd protein, len: 196 aa and low similarities to TR:CAA21729 (EMBL; AL032656) Caenorhabditis elegans Y106G6E.2 protein, 238 aa; fasta scores: opt: 120 z-score: 136.2 E():3.2; 29.3% identity in 116 aa overlap. Contains possible N-terminal region signal peptide sequence 2 1 3 3 [Reporter]SCO1973 (19N8)_PCR/550 [Gene]SCO1973/500 NC_003888 SC3C9.08c,conserved hypothetical protein, len: 264 aa; low similarity to SW:YT37_STRFR (EMBL:M29297) Streptomyces fradiae hypothetical 37.1 kDa protein in transposon TN4556, 345 aa; fasta scores: opt: 224 Z-score: 256.6 bits: 55.5 E(): 1.1e-06; 29.259% identity in 270 aa overlap 1 2 20 7 [Reporter]SCO0033 (16A7)_PCR/2440 [Gene]SCO0033/2219 NC_003888 SCJ4.14c, possible secreted neuraminidase (sialidase), len: 748 aa; similar to many, both prokaryote and eukaryote, egs. SW:NANH_MICVI sialidase precusor from Micromonospora viridifaciens (647 aa) fasta scores; opt: 274, z-score: 299.9, E(): 2.4e-09, (28.6% identity in 587 aa overlap) and TR:O35657 (EMBL:Y11412) G9 sialidase precusor from Mus musculus (Mouse) (409 aa) fasta scores; opt: 325, z-score: 358.5, E(): 1.3e-12, (26.6% identity in 425 aa overlap). Also similar to TR:Q9ZBW1 (EMBL:AL034443) putative neuraminidase from Streptomyces coelicolor (648 aa) fasta scores; opt: 479, z-score: 524.0, E(): 7.9e-22, (33.5% identity in 638 aa overlap). Contains possible N-terminal signal sequence. 1 2 20 6 [Reporter]SCO3312 (17M3)_PCR/2439 [Gene]SCO3312/2218 NC_003888 SCE68.10, hypothetical protein, len: 136 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Similar to TRNEW:CAC46514 (EMBL:AL591789) hypothetical protein SMC04265 from Rhizobium meliloti (Sinorhizobium meliloti) (147 aa) fasta scores; opt: 380, Z-score: 458.7, E(): 6.5e-18, 46.875% identity (47.244% ungapped) in 128 aa overlap. 1 2 20 5 [Reporter]SCO0596 (18I3)_PCR/2438 [Gene]SCO0596/2217 NC_003888 SCF55.20, probable DNA-binding protein, len: 187 aa; similar to SW:FTPA_HAEDU (EMBL:U18769) Haemophilus ducreyi fine tangled pili major subunit (24 KD surface protein) FtpA, 189 aa; fasta scores: opt: 559 z-score: 670.3 E(): 5.8e-30; 49.1% identity in 171 aa overlap and to SW:DPS_ECOLI (EMBL:X69337) Escherichia coli DNA protection during starvation protein Dps or PexB, 166 aa; fasta scores: opt: 276 z-score: 338.0 E(): 1.9e-11; 34.2% identity in 161 aa overlap. Contains match to Prosite entry PS00818 Dps protein family signature 1 1 2 20 4 [Reporter]SCO1665 (19E3)_PCR/2437 [Gene]SCO1665/2216 NC_003888 SCI52.07c, conserved hypothetical protein, len: 196 aa; similar to TR:O06241 (EMBL:Z95388)Mycobacterium tuberculosis hypothetical 21.2 kDa protein MTCY270.34, 195 aa; fasta scores: opt: 409 Z-score: 445.1 bits: 89.2 E(): 3.3e-17; 54.639% identity in 194 aa overlap 1 2 20 3 [Reporter]SCO4026 (20A3)_PCR/2436 [Gene]SCO4026/2215 NC_003888 2SC10A7.30c, possible ATP binding protein, len: 156 aa; similar to TR:Q9XAG1 (EMBL:AL079356) Streptomyces coelicolor hypothetical 15.6 kDa protein SC6G9.21, 144 aa; fasta scores: opt: 278 z-score: 351.7 E(): 4.4e-12; 42.5% identity in 120 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 2 20 2 [Reporter]SCO2813 (20I23)_PCR/2435 [Gene]SCO2813/2214 NC_003888 SCBAC17F8.04, conserved hypothetical protein, len: 188aa: similar to many e.g. TR:BAB48410 (EMBL:AP002996) MLR0926 protein from Rhizobium loti (188 aa) fasta scores; opt: 447, Z-score: 537.5, 38.587% identity (39.227% ungapped) in 184 aa overlap 1 2 19 22 [Reporter]SCO1345 (1I19)_PCR/2433 [Gene]SCO1345/2213 NC_003888 2SCG61.27c, fabG2, possible 3-oxoacyl-[acyl-carrier protein] reductase, len: 253 aa; similar to many eg. SW:P25716 (FABG_ECOLI) 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) from Escherichia coli (244 aa) fasta scores; opt: 474, z-score: 540.0, E(): 1.3e-22, 37.0% identity in 243 aa overlap. Contains Pfam match to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus, Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature. 1 2 19 21 [Reporter]SCO4785 (2E19)_PCR/2432 [Gene]SCO4785/2212 NC_003888 SCD63.17, guaA, GMP synthase, len: 526 aa; highly similar to SW:GUAA_CORAM (EMBL:Y10499) Corynebacterium ammoniagenes GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2) GuaA, 522 aa; fasta scores: opt: 2191 z-score: 2442.9 E(): 0; 65.7% identity in 528 aa overlap. Contains Pfam matches to entries PF00117 GATase, Glutamine amidotransferase class-I and PF00958 GMP_synt_C, GMP synthase C terminal domain and match to Prosite entry PS00442 Glutamine amidotransferases class-I active site 1 2 19 20 [Reporter]SCO7653 (3A19)_PCR/2431 [Gene]SCO7653/2211 NC_003888 SC10F4.26c, hypothetical fusion protein, len: 240 aa; N-terminal and C-terminal domains both similar to the same proteins, e.g. TR:Q9KZ80 (EMBL:AL354048) Streptomyces coelicolor hypothetical 15.9 kDa protein SCE25.06, 145 aa; blastp scores for N-terminal domain: Score = 130 (45.8 bits), Expect = 3.2e-11, Sum P(2) = 3.2e-11; Identities = 39/92 (42%), Positives = 50/92 (54%) and for C-terminal domain: Score = 72 (25.3 bits), Expect = 0.22, Sum P(2) = 0.20; Identities = 29/90 (32%), Positives = 44/90 (48%) 1 2 19 19 [Reporter]SCO7218 (4M15)_PCR/2430 [Gene]SCO7218/2210 NC_003888 SC2H12.17, possible iron transport lipoprotein, len: 345 aa. Similar to Streptomyces coelicolor TR:Q9RK12(EMBL:AL109974) putative iron-siderophore binding lipoprotein, SCF34.13C (350 aa), fasta scores opt: 221 z-score: 254.1 E(): 1.1e-06 28.7% identity in 352 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains a Pfam match to entry PF01497 Peripla_BP_2, Periplasmic binding protein 1 2 19 18 [Reporter]SCO7680 (5I15)_PCR/2429 [Gene]SCO7680/2209 NC_003888 SC4C2.15, possible ABC transporter ATP-binding protein, len: 541 aa; similar to TR:AAG05449 (EMBL:AE004632) Pseudomonas aeruginosa probable ATP-binding component of ABC transporter PA2061, 536 aa; fasta scores: opt: 861 z-score: 716.8 E(): 2.2e-32; 37.4% identity in 522 aa overlap. Contains 2x Pfam matches to entry PF00005 ABC_tran, ABC transporter and 2x matches to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and 2x to PS00211 ABC transporters family signature 1 2 19 17 [Reporter]SCO7772 (6E15)_PCR/2428 [Gene]SCO7772/2208 NC_003888 SC5E9.20c, unknown, len: 194 aa 1 2 19 16 [Reporter]SCO6166 (7A15)_PCR/2427 [Gene]SCO6166/2207 NC_003888 SC6C5.02, unknown, len: 272aa 1 2 19 15 [Reporter]SCO5749 (8M11)_PCR/2426 [Gene]SCO5749/2206 NC_003888 SC7C7.04, probable two-component regulator, len: 224 aa; similar to many e.g. PHOB_ECOLI phosphate regulon transcriptional regulator (229 aa), fasta scores; opt: 200 z-score: 349.0 E(): 3.3e-12, 28.2% identity in 216 aa overlap. Contains probable coiled-coil from 153 to 195 and Pfam match to entry response_reg PF00072, Response regulator receiver domain, score 125.68 1 2 19 14 [Reporter]SCO7008 (9I11)_PCR/2425 [Gene]SCO7008/2205 NC_003888 SC8F11.34, possible ABC-transport protein, len: 624. This protein contains a hydrophobic membrane spanning N-terminal domain and an ATP-binding C-terminal domain. Similar to many Eukaryotic and Prokaryotic putative transport proteins including: Drosophila melanogaster TR:O46057(EMBL:AL009147) EG:171D11.2 transporter protein (609 aa), fasta scores opt: 777 z-score: 850.8 E(): 0 31.0% identity in 597 aa overlap and Streptomyces coelicolor TR:Q9Z4Z6(EMBL:AL035707) putative ABC transporter, SCE29.04C (615 aa), fasta scores opt: 957 z-score: 1047.3 E():0 35.1% identity in 598 aa overlap. Contains a Prosite hits to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop), as well as Pfam matches to entries PF00005 ABC_tran, ABC transporter and PF00664 ABC_membrane, ABC transporter transmembrane region. Contains multiple possible membrane spanning hydrophobic domains. 1 2 19 13 [Reporter]SCO3799 (10E11)_PCR/2424 [Gene]SCO3799/2204 NC_003888 SCAC2.07c, unknown, len: 156 aa 1 2 19 12 [Reporter]SCO2498 (11A11)_PCR/2423 [Gene]SCO2498/2203 NC_003888 SCC121.01, probable efflux membrane protein, len: >344 aa; similar to SW:TCMA_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C resistance and export protein TcmA, 538 aa; fasta scores: opt: 654 z-score: 718.1 E(): 1.3e-32; 38.7% identity in 305 aa overlap,SCC82.04, possible efflux protein, len: >168 aa; similar to TR:CAB55652 (EMBL:AL117385) Streptomyces coelicolor putative transmembrane efflux protein, SC5G9.04c, 489 aa; fasta scores: opt: 564 z-score: 675.1 E(): 3.3e-30; 46.9% identity in 160 aa overlap and to SW:PUR8_STRLP (EMBL:X76855) Streptomyces lipmanii (Streptomyces alboniger)puromycin resistance protein Pur8, 503 aa; fasta scores: opt: 454 z-score: 544.8 E(): 5.9e-23; 44.7% identity in 159 aa overlap. Contains N-terminal region signal peptide sequence and match to Prosite entry PS00216 Sugar transport proteins signature 1 1 2 19 10 [Reporter]SCO3277 (13I7)_PCR/2421 [Gene]SCO3277/2202 NC_003888 SCE39.27c, possible phosphotransferase, len: 288aa; similar to many antibiotic phosphotransferases eg. TR:Q53826 (EMBL:U13078) capreomycin phosphotransferase from Streptomyces capreolus (281 aa) fasta scores; opt: 157, z-score: 183.5, E(): 0.0065, (28.3% identity in 226 aa overlap). 1 2 19 9 [Reporter]SCO1537 (14E7)_PCR/2420 [Gene]SCO1537/2201 NC_003888 SCL2.27c, probable transport system membrane protein, len: 303 aa; similar to SW:YESQ_BACSU (EMBL:Z99107) Bacillus subtilis probable ABC transporter permease protein YesQ, 296 aa; fasta scores:opt: 868 z-score: 1048.4 E(): 0; 45.1% identity in 295 aa overlap and to TR:CAB59597 (EMBL:AL132662) Streptomyces coelicolor probable sugar transport inner membrane protein SCF11.20, 299 aa; fasta scores: opt: 636 z-score: 769.8 E(): 0; 37.7% identity in 308 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 1 2 19 8 [Reporter]SCO1568 (15A7)_PCR/2419 [Gene]SCO1568/2200 NC_003888 SCL24.04c, probable tetR-family transcriptional regulator, len: 189 aa; similar to SW:NFXB_PSEAE (EMBL:X65646) Pseudomonas aeruginosa transcriptional regulatory protein NfxB, 187 aa; fasta scores: opt: 297 z-score: 367.8 E(): 4.5e-13; 33.3% identity in 174 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Contains also possible helix-turn-helix motif at residues 28..49 (+5.21 SD) 1 3 17 8 [Reporter]SCO1796 (15I2)_PCR/4391 [Gene]SCO1796/3999 NC_003888 SCI5.04c, putative secreted protein, len: 319 aa; similar to many e.g. SW:YS09_MYCTU hypothetical protein from Mycobacterium tuberculosis (381 aa) fasta scores; opt: 956, z-score: 915.0, E(): 0, 55.3% identity in 311 aa overlap. Contains Pfam match to PF01145 and Prosite match to PS01270 Band 7 protein family signature. Contains possible N-terminal region signal peptide sequence 1 3 17 7 [Reporter]SCO5016 (16E2)_PCR/4390 [Gene]SCO5016/3998 NC_003888 SCK15.18c, possible secreted protein, len: 271 aa. Contains possible N-terminal region signal peptide sequence and a repeat region: 7x AS at N-terminal domain 1 3 17 6 [Reporter]SCO0930 (17A2)_PCR/4389 [Gene]SCO0930/3997 NC_003888 SCM10.18c, possible lipoprotein, len: 317 aa. Contains possible N-terminal region signal peptide sequence and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site 1 3 17 5 [Reporter]SCO0111 (17M22)_PCR/4388 [Gene]SCO0111/3996 NC_003888 SCJ11.40, probable oxidoreductase, len: 753 aa; similar to many egs. TR:O32457 (EMBL:D87924) ORF7 from the putative polyketide synthase cluster of Actinomadura hibisca (246 aa) fasta scores; opt: 480, z-score: 498.5, E(): 2.1e-20, (38.3% identity in 248 aa overlap) and TR:Q54810 (EMBL:L35560) reductase from the doxorubicin polyketide synthase cluster of Streptomyces peucetius (251 aa) fasta scores; opt: 408, z-score: 425.7, E(): 2.4e-16, (33.7% identity in 243 aa overlap). Contains Pfam matches to entry PF00106 adh_short, short chain dehydrogenase and to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature 1 3 17 4 [Reporter]SCO4005 (18I22)_PCR/4387 [Gene]SCO4005/3995 NC_003888 2SC10A7.09, probable RNA polymerase sigma factor, len: 157 aa; similar to SW:RPOE_STRCO (EMBL:L29636) Streptomyces coelicolor RNA polymerase sigma-E factor SigE, 176 aa; fasta scores: opt: 451 z-score: 556.7 E(): 1.7e-23; 48.7% identity in 150 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) and possible helix-turn-helix motif at residues 120..141 (+3.36 SD) 1 3 17 3 [Reporter]SCO6108 (19E22)_PCR/4386 [Gene]SCO6108/3994 NC_003888 SCBAC1A6.32, fusH, esterase, len: 520 aa; identical to TR:Q54344 (EMBL:Z50108) Streptomyces lividans secreted esterase FusH, 520 aa. Contains Pfam match to entry PF00089 trypsin, Trypsin and a 3x degenerate repeat at C-terminal region: DLGRLYFADVDGDRKADMIVHT(S/G)DGN(I/V)EVRFNHGTYWDQGTHWSGGWGRF. Also contains possible hydrophobic membrane spanning region at N-terminal domain 1 3 17 2 [Reporter]SCO7492 (20M18)_PCR/4385 [Gene]SCO7492/3993 NC_003888 SCBAC17A6.25, unknown, len: 454 aa; no significant database matches. 1 3 16 22 [Reporter]SCO1210 (1M14)_PCR/4383 [Gene]SCO1210/3992 NC_003888 2SCG58.10, probable transcriptional regulatory protein, len: 218 aa; similar to TR:O53757 (EMBL:AL021933) Mycobacterium tuberculosis putative regulatory protein MTV038.16c, 234 aa; fasta scores: opt: 214 z-score: 258.1 E(): 6.5e-07; 30.7% identity in 199 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 35..56 (+3.27 SD) 1 3 16 21 [Reporter]SCO6800 (2I14)_PCR/4382 [Gene]SCO6800/3991 NC_003888 SC1A2.09, kbl, 2-amino-3-ketobutyrate coenzyme A ligase, len: 397 aa. Highly similar to many ligases involved in L-threonine catabolism e.g. Escherichia coli SW:KBL_ECOLI(EMBL:X06690) 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (398 aa), fasta scores opt: 1612 z-score: 1822.6 E():0 64.0% identity in 389 aa overlap. Contains a Prosite hit to PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site and a Pfam match to entry PF00222 aminotran_2, Aminotransferases class-II. 1 3 16 20 [Reporter]SCO2221 (3E14)_PCR/4381 [Gene]SCO2221/3990 NC_003888 SC10B7.16, hypothetical protein, len: 296 aa; similar to TR:O33319 (EMBL:AL008967) Mycobacterium tuberculosis hypothetical 40.2 kD protein MTV002.42c, 356 aa; fasta scores: opt: 980 z-score: 1190.8 E(): 0; 51.2% identity in 281 aa overlap 1 3 16 19 [Reporter]SCO5480 (4A14)_PCR/4380 [Gene]SCO5480/3989 NC_003888 SC2A11.14, probable oligopeptide ABC transporter ATP-binding protein, len: 496 aa; similar to many e.g. APPF_BACSU oligopeptide transport ATP-binding protein (329 aa), fasta scores; opt: 1284 z-score: 1056.3 E(): 0, 56.4% identity in 326 aa overlap Also similar to S. coelicolor TR:P72410 (EMBL:U68036) bldKE (284 aa), fasta scores; opt: 1114 z-score: 1091.6 E(): 0, 59.3% identity in 280 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature and Pfam match to entry PF00005 ABC_tran, ABC transporters, score 202.90, E-value 4.8e-57. C-terminus contains 13 degenerate repeats of AS(D /S)DA 1 3 16 18 [Reporter]SCO2094 (5M10)_PCR/4379 [Gene]SCO2094/3988 NC_003888 SC4A10.27, possible regulatory protein, len: 337 aa; similar to many e.g. SW:MOXR_PARDE (EMBL:M57684), MoxR, Paracoccus denitrificans protein probably involved in the regulation of methanol dehydrogenase synthesis (339 aa), fasta scores; opt: 189 z-score:216.0 E(): 7.6e-05, 31.55% identity in 336 aa overlap. Similar to many hypothetical proteins e.g. TM1132 (EMBL:AE001771) Thermotoga maritima putative MoxR protein (305 aa) (52.1% identity in 303 aa overlap). Similar to TR:Q9Z538 (EMBL:AL035212) S.coelicolor possible regulatory protein (332 aa) (45.8% identity in 312 aa overlap) 1 3 16 17 [Reporter]SCO6517 (6I10)_PCR/4378 [Gene]SCO6517/3987 NC_003888 SC5C7.02, uvrA-like protein, len: 796 aa; similar to Streptomyces peucetius TR:Q54827 (EMBL:L76359) daunorubicin resistance protein drrC (764 aa), fasta scores; opt: 2051 z-score: 2702.2 E(): 0, 51.1% identity in 775 aa overlap, and to many uvrA proteins e.g. UVRA_NEIGO excinuclease ABC subunit A (950 aa), fasta scores; opt: 412 z-score: 1697.0 E(): 0, 34.1% identity in 953 aa overlap. Contains 2x PS00017 ATP /GTP-binding= match to entry PF00005 ABC_tran, ABC transporters, scores 42.40, E-value 1.7e-10 and 71.20, E-value 2.2e-17 1 3 16 16 [Reporter]SCO5688 (7E10)_PCR/4377 [Gene]SCO5688/3986 NC_003888 SC5H4.12, probable transport system integral membrane protein, len: 323 aa; similar to SW:LPLC_BACSU (EMBL:L19164) Bacillus subtilis LplC, 295 aa; fasta scores: opt: 669 z-score: 758.9 E(): 0; 38.0% identity in 276 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Contains also possible hydrophobic membrane spanning regions 1 3 16 15 [Reporter]SCO5314 (8A10)_PCR/4376 [Gene]SCO5314/3985 NC_003888 SC6G9.19, whiE ORF VII, len: 111 aa; unknown function, whiE locus protein previously sequenced from S. coelicolor as SW:YW12_STRCO (EMBL:X55942) 1 3 16 14 [Reporter]SCO7837 (9M6)_PCR/4375 [Gene]SCO7837/3984 NC_003888 SC8E7.34c, possible membrane protein, len: 363 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1Y4(EMBL:AL109973) hypothetical protein, SCJ30.05 (363 aa). Also similar to Streptomyces coelicolor TR:Q9X886(EMBL:AL049707) putative transferase, SCE15.11C (448 aa), fasta scores opt: 411 z-score: 409.1 E(): 2.6e-15 32.8% identity in 274 aa overlap. Note the large overlap with the adjoining CDS. Contains possible membrane spanning hydrophobic domains and a TTA /leucine codon, possible target for bldA regulation. 1 3 16 13 [Reporter]SCO2532 (10I6)_PCR/4374 [Gene]SCO2532/3983 NC_003888 SCC117.05, PhoH-like protein, len: 359 aa. Highly similar to many including: Escherichia coli SW:PHOH_ECOLI (EMBL:D10391) phosphate starvation-inducible protein PhoH protein (354 aa), fasta scores opt: 583 z-score: 650.4 E(): 7.8e-29 37.7% identity in 321 aa overlap and Mycobacterium tuberculosis SW:PHOL_MYCTU(EMBL:Z95208) PhoH-like protein (352 aa), fasta scores opt: 1407 z-score: 1560.0 E():0 63.6% identity in 349 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 16 12 [Reporter]SCO2287 (11E6)_PCR/4373 [Gene]SCO2287/3982 NC_003888 SCC75A.33, possible oxidoreductase, len: 263 aa. Similar to several including: Bacillus megaterium SW:DHG2_BACME (EMBL:D90044) glucose 1-dehydrogenase II (EC 1.1.1.47) (261 aa), fasta scores opt: 400 z-score: 442.6 E(): 2.8e-17 38.9% identity in 193 aa overlap SW:YOHF_ECOLI (EMBL:U00007) hypothetical oxidoreductase (253 aa), fasta scores opt: 422 z-score: 466.5 E(): 1.3e-18 38.3% identity in 206 aa overlap. Contains a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature and a Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 1 3 16 11 [Reporter]SCO4801 (12A6)_PCR/4372 [Gene]SCO4801/3981 NC_003888 SCD63A.12c, unknown, len: 606 aa. High content in alanine amino acid residues 1 3 16 10 [Reporter]SCO3035 (13M2)_PCR/4371 [Gene]SCO3035/3980 NC_003888 SCE34.16, hypothetical protein, len: 164 aa; similar to TR:O53413 (EMBL:AL021897) Mycobacterium tuberculosis hypothetical 20.6 kD protein MTV017.18, 188 aa; fasta scores: opt: 251 z-score: 321.5 E(): 1.9e-10; 39.6% identity in 154 aa overlap 1 3 16 9 [Reporter]SCO3807 (14I2)_PCR/4370 [Gene]SCO3807/3979 NC_003888 SCGD3.08c, possible hydrolase, len: 264 aa; weakly similar to hydrolases e.g. to TR:Q44185 (EMBL:X91070) Agrobacterium radiobacter D-N-alpha-carbamilase (304 aa) (28.17% identity in 252 aa overlap). Similar to many hypothetical proteins e.g. TR:O27839 (EMBL:AE000934) Methanobacterium thermoautotrophicum putative N-carbamoyl-D-amino acid amidohydrolase (272 aa) (36.2% identity in 265 aa overlap). Similar to others from S.coelicolor e.g. TR:O69808 (EMBL:AL023496) S.coelicolor possible hydrolase (280 aa) (31.7% identity in 281 aa overlap). An alternative start codon is present at codon 10. Contains PS01227 Uncharacterized protein family UPF0012 signature which is also present in many of the hypothetical proteins similar to this one 1 3 16 8 [Reporter]SCO3628 (15E2)_PCR/4369 [Gene]SCO3628/3978 NC_003888 SCH10.06c, putative secreted protein, len: 163 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains possible N-terminal region signal peptide sequence 1 3 16 7 [Reporter]SCO1566 (16A2)_PCR/4368 [Gene]SCO1566/3977 NC_003888 SCL24.02, possible acyltransferase, len: 223 aa; similar to TR:Q9ZBS1 (EMBL:AL034447) Streptomyces coelicolor putative acyltransferase SC7A1.02, 264 aa; fasta scores: opt: 277 z-score: 327.1 E(): 8.5e-11; 30.7% identity in 202 aa overlap and to TR:CAB51970 (EMBL:AL109661) Streptomyces coelicolor hypothetical 28.2 kD protein SC6E10.16c, 262 aa; fasta scores: opt: 858 z-score: 879.9 E(): 0; 59.0% identity in 217 aa overlap. Contains Pfam match to entry PF01553 Acyltransferase, Acyltransferase 1 3 16 6 [Reporter]SCO0708 (16M22)_PCR/4367 [Gene]SCO0708/3976 NC_003888 SCF42.18c, possible branched-chain amino acid ABC transport protein, len: 299 aa. Similar to many other transport proteins including: Pseudomonas aeruginosa SW:BRAD_PSEAE(EMBL:D90223) high-affinity branched-chain amino acid transport protein, BraD (307 aa), fasta scores opt: 318 z-score: 364.2 E(): 6.8e-13 28.5% identity in 312 aa overlap and Deinococcus radiodurans TR:AAF09863(EMBL:AE001889) branched-chain amino acid ABC transporter, permease protein (285 aa), fasta scores opt: 432 z-score: 492.1 E(): 5.1e-20 34.6% identity in 295 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 1 3 16 5 [Reporter]SCO3639 (17I22)_PCR/4366 [Gene]SCO3639/3975 NC_003888 SCH10.17c, probable two-component system sensor kinase, len: 472 aa; similar to many e.g. TR:Q53893 (EMBL:U51332), AbsA1, Streptomyces coelicolor histidine kinase homolog involved in negative regulation of antibiotic synthesis (571 aa), fasta scores; opt: 370 z-score: 410.4 E(): 1.5e-15, 29.6% identity in 375 aa overlap. Also similar to SW:NARX_ECOLI (EMBL:X13360), NarX, NarR, Escherichia coli nitrate/nitrite sensor protein (598 aa) (28.5% identity in 228 aa overlap). Contains hydrophobic, probable membrane-spanning regions. Similar to many other putative two-component system sensor kinases from Streptomyces coelicolor e.g. SCH10.32c, probable two-component system sensor (441 aa) (38.0% identity in 389 aa overlap). Contains probable helix-turn-helix motif at aa 235-256 (Score 1122, +3.01 SD). The start codon may be further downstream, which would agree with some other bacterial two-component system sensors; however, NarX has a length comparable to this prediction 1 3 16 4 [Reporter]SCO4862 (18E22)_PCR/4365 [Gene]SCO4862/3974 NC_003888 SCK20.03, unknown, len: 189 aa 4 4 11 19 [Reporter]SCO6621 (4P5)_PCR/7797 [Gene]SCO6621/7099 NC_003888 SC1F2.18, unknown, len: 424 aa; some similarity to C-terminus of TR:O54229 (EMBL:AJ223176) Streptomyces granaticolor Ser/Thr protein kinase (780 aa), fasta scores; opt: 260 z-score: 221.0 E(): 4.5e-05, 27.8% identity in 439 aa overlap 1 3 16 3 [Reporter]SCO1882 (19A22)_PCR/4364 [Gene]SCO1882/3973 NC_003888 SCI39.29c, probable secreted sugar-binding protein, len: 452 aa; similar to TR:P72397 (EMBL:Y07706) Streptomyces coelicolor putative maltose-binding protein MalE, 423 aa; fasta scores: opt: 433 Z-score: 459.6 bits: 94.2 E(): 5.2e-18; 30.716% identity in 433 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and possible N-terminal region signal peptide sequence 4 4 11 18 [Reporter]SCO6575 (5L5)_PCR/7796 [Gene]SCO6575/7098 NC_003888 SC3F9.10, unknown, len: 315 aa; some similarity to MOCC_RHIME rhizopine catabolism protein MocC (325 aa), fasta scores; opt: 127 z-score: 216.9 E(): 7.2e-05, 33.5% identity in 167 aa overlap 4 1 21 18 [Reporter]SCO7109 (5D20)_PCR/1969 [Gene]SCO7109/1799 NC_003888 SC4B10.10c, possible oxidoreductase, len: 576 aa; similar to TR:O27546 (EMBL:AE000910) Methanobacterium thermoautotrophicum succinate dehydrogenase, flavoprotein subunit MTH1502, 558 aa; fasta scores: opt: 1262 z-score: 1392.4 E(): 0; 41.0% identity in 542 aa overlap and to TR:O53141 (EMBL:AJ000941) Methanobacterium thermoautotrophicum strain marburg, thiol:fumarate reductase subunit A Tfr, 545 aa; fasta scores: opt: 1184 z-score: 1306.6 E(): 0; 40.5% identity in 536 aa overlap. Contains Pfam match to entry PF00890 FAD_binding_2, FAD binding domain 1 3 16 2 [Reporter]SCO5970 (20I18)_PCR/4363 [Gene]SCO5970/3972 NC_003888 SCBAC16H6.05, unknown, len: 156 aa: no significant database matches. Contains TTA-encoded leucine close to C-terminus. 4 4 11 17 [Reporter]SCO2039 (6H5)_PCR/7795 [Gene]SCO2039/7097 NC_003888 SC4G6.08c, trpC1, indoleglycerol phosphate synthase, len: 269 aa; previously sequenced therefore identical to TR:O68814 (EMBL:AF054585). Also similar to many others eg. SW:TRPC_AZOBR trpC, indoleglycerol phosphate synthase from Azospirillum brasilense (262 aa) fasta scores; opt: 589, z-score: 655.0, E(): 3.7e-29, (44.4% identity in 259 aa overlap). Contains Pfam match to entry PF00218 IGPS, Indole-3-glycerol phosphate synthases and Prosite match to PS00614 Indole-3-glycerol phosphate synthase signature. 4 1 21 17 [Reporter]SCO2176 (6P16)_PCR/1968 [Gene]SCO2176/1798 NC_003888 SC5F7.25c, possible integral membrane protein, len: 246 aa; similar to TR:O53514 (EMBL:AL021957) putative integral membrane protein from Mycobacterium tuberculosis (295 aa) fasta scores; opt: 468, z-score: 539.2, E(): 1.1e-22, (42.3% identity in 260 aa overlap). Contains possible membrane spanning hydrophobic regions. Also contains Pfam match to entry PF00499 oxidored_q3, NADH-ubiquinone/plastoquinone oxidoreductase chain 6 1 3 15 22 [Reporter]SCO1206 (1I14)_PCR/4361 [Gene]SCO1206/3971 NC_003888 2SCG58.06, possible polyketide synthase, len: 374 aa; highly similar to TR:Q54240 (EMBL:AB018074) Streptomyces griseus ORF-1 protein similar to chalcone synthase, 372 aa; fasta scores: opt: 1769 z-score: 1998.4 E(): 0; 70.4% identity in 361 aa overlap and to SW:CHSJ_PETHY (EMBL:X14597) Petunia hybrida chalcone synthase J (EC 2.3.1.74) ChsJ, 389 aa; fasta scores: opt: 412 z-score: 469.3 E(): 1.1e-18; 29.5% identity in 312 aa overlap. Contains Pfam match to entry PF00195 Chal_stil_synt, Chalcone and stilbene synthases 4 4 11 16 [Reporter]SCO7283 (7D5)_PCR/7794 [Gene]SCO7283/7096 NC_003888 SC5H1.09c, possible hydroxylase, len: 265 aa; similar to e.g. TR:Q55078 (EMBL:U50973), doxA, Streptomyces sp. daunomycin C-14 hydroxylase (275 aa), fasta scores; opt: 440 z-score: 519.4 E(): 1.3e-21, 37.5% identity in 259 aa overlap. Similar to SC9B10.20c (EMBL:AL009204) S.coelicolor hypothetical protein (267 aa) (34.1% identity in 249 aa overlap) 4 1 21 16 [Reporter]SCO2113 (7L16)_PCR/1967 [Gene]SCO2113/1797 NC_003888 SC6E10.07, bfr, probable bacterioferritin, len: 167 aa; highly similar to SW:BFR_MYCAV (EMBL:X76906) Bfr, Mycobacterium avium bacterioferritin (159 aa), fasta scores; opt: 672 z-score: 815.6 E(): 0, 63.6% identity in 162 aa overlap. Contains Pfam match to entry PF01334 Bacteriofer, Bacterioferritin 1 3 15 21 [Reporter]SCO5676 (2E14)_PCR/4360 [Gene]SCO5676/3970 NC_003888 SC8B7.02, gabT, probable 4-aminobutyrate aminotransferase, len: 444 aa; highly similar to e.g. GABT_ECOLI 4-aminobutyrate aminotransferase (EC 2.6.1.19) (426 aa), fasta s cores; opt: 1194 z-score: 1404.8 E(): 0, 45.5% identity in 424 aa overlap. Contains PS00600 Aminotransferases class-II I pyridoxal-phosphate attachment site and Pfam match to ent ry PF00202 aminotran_3, Aminotransferases class-III pyridox al-phosphate, score 516.20, E-value 5e-179 4 4 11 14 [Reporter]SCO2642 (9L1)_PCR/7792 [Gene]SCO2642/7095 NC_003888 SC8E4A.12c, unknown, len: 158 aa 4 1 21 15 [Reporter]SCO6851 (8H16)_PCR/1966 [Gene]SCO6851/1796 NC_003888 SC7F9.03c, unknown, len: 188 aa. Weakly similar to Streptomyces coelicolor TR:Q9X874 (EMBL:AL049661) hypothetical protein, SCE134.19 (211 aa), fasta scores opt: 124 z-score: 162.0 E(): 0.13 24.0% identity in 150 aa overlap. 4 4 11 13 [Reporter]SCO6481 (10H1)_PCR/7791 [Gene]SCO6481/7094 NC_003888 SC9C7.17c, conserved hypothetical protein, len: 348aa; similar to several hypothetical proteins eg. SW:Y0BN_MYCTU from Mycobacterium tuberculosis (340 aa) fasta scores; opt: 998, z-score: 1321.8, E(): 0, (47.6% identity in 355 aa overlap). 4 1 21 14 [Reporter]SCO7542 (9D16)_PCR/1965 [Gene]SCO7542/1795 NC_003888 SC8G12.18c, possible secreted protein, len: 448 aa. Contains a possible N-terminal signal sequence. 4 4 11 12 [Reporter]SCO2705 (11D1)_PCR/7790 [Gene]SCO2705/7093 NC_003888 SCC61A.26c, possible membrane protein, len: 88 aa; similar to others from Streptomyces coelicolor eg. TR:CAB75306 (EMBL:AL139164) putative small secreted protein (75 aa) fasta scores; opt: 330, z-score: 389.5, E(): 3.1e-14, 72.1% identity in 68 aa overlap and neighbouring CDS SCC61A.20. Contains possible hydrophobic membrane spanning region 4 1 21 13 [Reporter]SCO1921 (10P12)_PCR/1964 [Gene]SCO1921/1794 NC_003888 SCC22.03c, possible aminotransferase, len: 418 aa; similar to many proposed aminotransferase/nifS-like proteins e.g. TR:Q49690 (EMBL:U00013) NifS from Mycobacterium leprae (418 aa) fasta scores; opt: 1438, z-score: 1673.0, E(): 0, (53.4% identity in 414 aa overlap). Contains Pfam match to entry PF00266 aminotran_5, Aminotransferases class-V. 4 4 11 11 [Reporter]SCO4674 (11P21)_PCR/7789 [Gene]SCO4674/7092 NC_003888 SCD40A.20, hypothetical protein, len: 145 aa; similar to TR:O06633 (EMBL:Z95618) Mycobacterium tuberculosis hypothetical 12.5 kD protein MTCY07H7A.08c, 115 aa; fasta scores: opt: 249 z-score: 314.3 E(): 4.5e-10; 39.5% identity in 119 aa overlap 4 1 21 12 [Reporter]SCO4561 (11L12)_PCR/1963 [Gene]SCO4561/1793 NC_003888 SCD16A.22, possible NLP/P60 family protein, len: 277aa; similar to many eg. TR:CAB39862 (EMBL:AL049497) putative secreted protein from Streptomyces coelicolor (338 aa) fasta scores; opt: 386, z-score: 364.3, E(): 5.8e-13, (35.1% identity in 251 aa overlap) and the C-terminal half of many Listeria spp. extracellular proteins eg. SW:P60_LISIN P60 Listeria innocua (481 aa) fasta scores; opt: , z-score: 328.5, E(): 5.7e-11, (28.9% identity in 242 aa overlap). Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family. Also contains coiled-coil region at 92-125aa and possible hydrophobic membrane spanning region 4 4 11 10 [Reporter]SCO3529 (12L21)_PCR/7788 [Gene]SCO3529/7091 NC_003888 SCE2.10c, unknown, len: 173aa; 4 1 21 11 [Reporter]SCO4485 (12H12)_PCR/1962 [Gene]SCO4485/1792 NC_003888 SCD69.05c, possible transcriptional regulator, len: 341 aa; similar to TR:CAB81864 (EMBL:AL161691) Streptomyces coelicolor putative deoR-family transcriptional regulator SCD40A.19c, 339 aa; fasta scores: opt: 480 z-score: 514.9 E(): 3.2e-21; 39.3% identity in 321 aa overlap. Contains possible helix-turn-helix motif at residues 21..42 (+5.42 SD) 4 4 11 9 [Reporter]SCO0067 (13H21)_PCR/7787 [Gene]SCO0067/7090 NC_003888 SCJ4.48, probable integral membrane permease, len: 273 aa; similar to many e.g. TR:Q9Z3R7 (EMBL:AF045609) putative inner membrane permease from the alpha-glucosides binding protein dependent transport system of Rhizobium (Sinorhizobium) meliloti (305 aa) fasta scores; opt: 393, z-score: 457.6, E(): 4e-18, (32.3% identity in 229 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Contains possible membrane spanning hydrophobic regions. 4 1 21 10 [Reporter]SCO3013 (13D12)_PCR/1961 [Gene]SCO3013/1791 NC_003888 SCE33.15c, probable two-component system response regulator, len: 229 aa; similar to TR:Q50447 (EMBL:Z95121) Mycobacterium tuberculosis MtrA, 228 aa; fasta scores: opt: 1141 z-score: 1397.7 E(): 0; 74.9% identity in 227 aa overlap, to SW:AFQ1_STRCO (EMBL:D10654) Streptomyces coelicolor transcriptional regulatory protein AfsQ1, 225 aa; fasta scores: opt: 698 z-score: 858.5 E(): 0; 47.7% identity in 220 aa overlap and to many other S. coelicolor two-component system response regulators, e.g. TR: CAB77324(EMBL:AL160331) putative response regulator SCD8A.03, 223 aa; fasta scores: opt: 695 z-score: 745.0 E(): 0; 50.2% identity in 223 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 4 1 21 9 [Reporter]SCO0936 (14P8)_PCR/1960 [Gene]SCO0936/1790 NC_003888 SCM10.24, probable oligosaccharide deacetylase, len: 201 aa; similar to SW:NODB_RHIGA (EMBL:X87578) Rhizobium galegae chitooligosaccharide deacetylase (EC 3.5.1.-) (nodulation protein B) NodB, 214 aa; fasta scores: opt: 461 z-score: 581.4 E(): 5.4e-25; 45.1% identity in 153 aa overlap. Contains Pfam match to entry PF01522 Polysac_deacet, Polysaccharide deacetylase 1 3 15 20 [Reporter]SCO2217 (3A14)_PCR/4359 [Gene]SCO2217/3969 NC_003888 SC10B7.12, probable secreted protein, len: 501 aa; similar to TR:CAB61161 (EMBL:AL132973) Streptomyces coelicolor putative membrane protein, SCF91.04c, 718 aa; fasta scores: opt: 1496 z-score: 1486.8 E(): 0; 55.1% identity in 523 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 15 19 [Reporter]SCO6948 (4M10)_PCR/4358 [Gene]SCO6948/3968 NC_003888 SC6F7.01, unknown, partial CDS, len:> 157 aa. Weakly similar to several including: Aeropyrum pernix TR:Q9YFC8 (EMBL:AP000059) hypothetical protein (168 aa), fasta scores opt: 231 z-score: 293.6 E(): 6.5e-09 35.8% identity in 137 aa overlap.,SC1G8.20, unknown, len: 183 aa. Weakly similar to several including: Aeropyrum pernix TR:Q9YFC8 (EMBL:AP000059) hypothetical protein (168 aa), fasta scores opt: 231 z-score: 290.8 E(): 9.8e-09 35.8% identity in 137 aa overlap. Overlaps and extends into CDS SC6F7.01 on the adjoining cosmid. 1 3 15 18 [Reporter]SCO2090 (5I10)_PCR/4357 [Gene]SCO2090/3967 NC_003888 SC4A10.23c, ftsI, cell division protein, len: 654 aa; previously sequenced as TR:Q9Z5V7 (EMBL:AF123319), FtsI, Streptomyces coelicolor cell division protein (651 aa) and identical to that sequence, but with a different putative start codon. C-terminal end shares weak similarity with others from S.coelicolor e.g. SC6G9.32 (EMBL:AL079356) S.coelicolor possible penicillin-binding protein (485 aa) (29.3% identity in 447 aa overlap). Contains a hydrophobic, possible membrane-spanning region. Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and two PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 15 17 [Reporter]SCO5789 (6E10)_PCR/4356 [Gene]SCO5789/3966 NC_003888 SC4H2.10c, unknown, len: 97 aa 1 3 15 16 [Reporter]SCO5684 (7A10)_PCR/4355 [Gene]SCO5684/3965 NC_003888 SC5H4.08, probable two-component system response regulator, len: 237 aa; similar to TR:Q9X8Q7 (EMBL:AL049754) Streptomyces coelicolor putative two-component system regulator SCH10.18c, 228 aa; fasta scores: opt: 780 z-score: 922.5 E(): 0; 58.7% identity in 225 aa overlap and to TR:BAA88407 (EMBL:AB016841) Streptomyces thermoviolaceus ChiR protein, 213 aa; fasta scores: opt: 567 z-score: 674.0 E(): 4.4e-30; 46.9% identity in 213 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature and also a possible helix-turn-helix motif at residues 180..201 (+2.70 SD) 1 3 15 15 [Reporter]SCO2467 (8M6)_PCR/4354 [Gene]SCO2467/3964 NC_003888 SC7A8.06, hypothetical protein, len: 333 aa; identical to previously sequenced TR:CAB51552 (EMBL:AJ244019) Streptomyces coelicolor A3(2) hypothetical 37.1 kD protein, 333 aa 4 4 11 8 [Reporter]SCO5005 (14D21)_PCR/7786 [Gene]SCO5005/7089 NC_003888 SCK15.07, unknown, len: 203 aa 1 3 15 14 [Reporter]SCO7831 (9I6)_PCR/4353 [Gene]SCO7831/3963 NC_003888 SC8E7.28c, unknown, len: 67 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1X7(EMBL:AL109973) hypothetical 7.2 kd protein, SCJ30.12 (67 aa). 4 4 11 7 [Reporter]SCO0760 (15P17)_PCR/7785 [Gene]SCO0760/7088 NC_003888 SCF81.19, probable methyltransferase, len: 220 aa; similar to TR:O27384 (EMBL:AE000896) Methanobacterium thermoautotrophicum methyltransferase related protein MTH1329, 196 aa; fasta scores: opt: 454 z-score: 525.3 E(): 6.9e-22; 44.2% identity in 190 aa overlap and to TR:Q53742 (EMBL:X92429) Streptomyces lipmanii (Streptomyces alboniger) N-methyl-transferase Pur5, 228 aa; fasta scores: opt: 228 z-score: 268.6 E(): 1.4e-07; 33.3% identity in 192 aa overlap. Contains match to Prosite entry PS00092 N-6 Adenine-specific DNA methylases signature 4 1 21 8 [Reporter]SCO0531 (15L8)_PCR/1959 [Gene]SCO0531/1789 NC_003888 SCF11.11, probable sugar transporter sugar binding protein, len: 422 aa; similar to TR:P72397 (EMBL:Y07706) Streptomyces coelicolor MalE, putative maltose-binding protein, 423 aa; fasta scores: opt: 367 z-score: 390.0 E(): 2.4e-14; 27.2% identity in 419 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, bacterial extracellular solute-binding protein and a prosite match correctly situated to entry PS00013 prokaryotic membrane lipoprotein lipid attachment site. Contains probable N-terminal signal sequence 1 3 15 13 [Reporter]SCO5709 (10E6)_PCR/4352 [Gene]SCO5709/3962 NC_003888 SC9F2.07c, probable tRNA pseudouridine synthase, len: 301 aa; similar to many e.g. SW:TRUB_ECOLI tRNA pseudouridine synthase B from Escherichia coli (314 aa) fasta scores; opt: 357, z-score: 415.7, E(): 7.6e-16, (37.6% identity in 234 aa overlap). 4 4 11 6 [Reporter]SCO2876 (16L17)_PCR/7784 [Gene]SCO2876/7087 NC_003888 SCE6.13, possible acetyltransferase, len: 208 aa. Similar to several including: Escherichia coli SW:ATDA_ECOLI(EMBL:D25276) spermidine N1-acetyltransferase (EC 2.3.1.57) (185 aa), fasta scores opt: 208 z-score: 273.6 E(): 8.2e-08 25.3% identity in 158 aa overlap and Streptomyces coelicolor TR:CAB76075(EMBL:AL157953) putative acetyltransferase, SCL11.12C (232 aa), fasta scores opt: 287 z-score: 372.5 E(): 2.6e-13 33.7% identity in 187 aa overlap. Contains a Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 4 1 21 7 [Reporter]SCO1894 (16H8)_PCR/1958 [Gene]SCO1894/1788 NC_003888 SCI7.12c, alanine-rich protein, len: 390aa; 1 3 15 12 [Reporter]SCO2283 (11A6)_PCR/4351 [Gene]SCO2283/3961 NC_003888 SCC75A.29c, possible secreted esterase, len: 351 aa. Similar, in parts, to several esterases e.g. Pseudomonas sp. TR:Q52467(EMBL:M68491) esterase (389 aa), fasta scores opt: 256 z-score: 289.8 E(): 9.2e-09 30.3% identity in 323 aa overlap. Also similar, in parts, to several penicillin-binding proteins e.g. Nocardia lactamdurans SW:PBP4_NOCLA (EMBL:Z13972) penicillin-binding protein 4 (PBP-4) (381 aa), fasta scores opt: 249 z-score: 282.1 E(): 2.4e-08 29.2% identity in 308 aa overlap. Contains a possible N-terminal signal sequence. 4 4 11 5 [Reporter]SCO0457 (17H17)_PCR/7783 [Gene]SCO0457/7086 NC_003888 SCF51A.35, unknown, len: 644 aa; 4 1 21 6 [Reporter]SCO0667 (17D8)_PCR/1957 [Gene]SCO0667/1787 NC_003888 SCF91.27c, putative secreted protein, len: 293 aa. Similar to several proteins of undefined function e.g. Mycobacterium tuberculosis TR:O53526(EMBL:AL021925) hypothetical 32.8 KD protein (309 aa), fasta scores opt: 441 z-score: 476.5 E(): 3.6e-19 32.8% identity in 299 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 15 11 [Reporter]SCO4120 (12M2)_PCR/4350 [Gene]SCO4120/3960 NC_003888 SCD72A.06, conserved hypothetical protein, len: 552 aa; similar in regions to many from Streptomyces coelicolor eg. TR:Q9ZBJ0 (EMBL:AL035161) conserved hypothetical protein (860 aa) fasta scores; opt: 906, z-score: 1019.8, E(): 0, 43.5% identity in 370 aa overlap 4 4 11 4 [Reporter]SCO5011 (18D17)_PCR/7782 [Gene]SCO5011/7085 NC_003888 SCK15.13, possible integral membrane protein, len: 300 aa; similar to TR:O27061 (EMBL:AE000871) Methanobacterium thermoautotrophicum conserved protein MTH980, 313 aa; fasta scores: opt: 235 z-score: 285.2 E(): 2e-08; 27.7% identity in 238 aa overlap. Contains possible N-terminal region signal peptide sequence and possible hydrophobic membrane spanning regions 4 1 21 5 [Reporter]SCO1843 (18P4)_PCR/1956 [Gene]SCO1843/1786 NC_003888 SCI8.28c, possible secreted protein, len: 411 aa; similar to TR:Q9RRL1 (EMBL:AE002077) Deinococcus radiodurans hypothetical protein DR2478, 407 aa; fasta scores: opt: 516 Z-score: 552.7 E(): 3.8e-23; 30.469% identity in 384 aa overlap. Contains a Prosite hit to PS00120 Lipases, serine active site. Contains a possible N-terminal signal sequence 4 4 11 2 [Reporter]SCO5111 (20H13)_PCR/7780 [Gene]SCO5111/7084 NC_003888 SCBAC31E11.07, possible GTP-binding protein, len: 635 aa; similar to SW:TYPA_ECOLI (EMBL:L19201) Escherichia coli GTP-binding protein TypA/BipA (tyrosine phosphorylated protein A) TypA or BipA or B3871, 591 aa; fasta scores: opt: 915 Z-score: 968.6 bits: 189.3 E(): 2.5e-46; 48.039% identity in 612 aa overlap. Contains Pfam match to entry PF00009 GTP_EFTU, Elongation factor Tu family and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00301 GTP-binding elongation factors signature 4 1 21 4 [Reporter]SCO6291 (19L4)_PCR/1955 [Gene]SCO6291/1785 NC_003888 SCBAC8D1.04, probable secreted oxidase, len: 511 aa; similar to SW:OOXA_RHIME (EMBL:U66830) Rhizobium meliloti opine oxidase subunit A (EC 1.*.*.*) OxaA, 526 aa; fasta scores: opt: 519 Z-score: 515.9 bits: 105.1 E(): 3.8e-21; 31.638% identity in 531 aa overlap. Contains 3x degenerate repeat: ASPVSA 4 4 10 22 [Reporter]SCO5269 (1H9)_PCR/7778 [Gene]SCO5269/7083 NC_003888 2SC7G11.31, unknown, len: 745 aa. Contains 2 conserved repeat regions: 2x GENPEY and 4x GHGAYP 4 1 21 3 [Reporter]SCP1.24 (20H4)_PCR/1954 [Gene]SCP1.24/1784 NC_003888 SCP1.24, unknown, doubtful CDS, len: 81aa; 4 4 10 21 [Reporter]SCO2228 (2D9)_PCR/7777 [Gene]SCO2228/7082 NC_003888 SC10B7.23c, aglA, alpha-glucosidase, len: 577 aa; highly similar to TR:P72463 (EMBL:Y08304) Streptomyces limosus alpha-glucosidase AglA, 391 aa; fasta scores: opt: 1453 z-score: 1607.1 E(): 0; 72.0% identity in 347 aa overlap, to TR:AAD50603 (EMBL:AF096282) Thermus aquaticus alpha-glucosidase, 529 aa; fasta scores: opt: 925 z-score: 1022.3 E(): 0; 40.2% identity in 567 aa overlap and to SW:MAL2_DROVI (EMBL:AF006573) Drosophila virilis maltase 2 precursor (EC 3.2.1.20) Mal2, 524 aa; fasta scores: opt: 870 z-score: 961.6 E(): 0; 36.2% identity in 516 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase 4 1 21 2 [Reporter]SCO7485 (20P24)_PCR/1953 [Gene]SCO7485/1783 NC_003888 SCBAC17A6.18c, possible oxidoreductase, len: 228aa; similar to many eg. TR:Q9L2J5 (EMBL:AL137165) putative oxidoreductase SCF42.24 from Streptomyces coelicolor (295 aa) fasta scores; opt: 450, Z-score: 508.6, 43.850% identity (45.304% ungapped) in 187 aa overlap and TR:BAB49857 (EMBL:AP003000) probable oxidoreductase from Rhizobium loti (319 aa) fasta scores; opt: 243, Z-score: 278.7, 31.980% identity (35.795% ungapped) in 197 aa overlap. Contains Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family, NAD-binding Rossmann fold. 4 4 10 20 [Reporter]SCO4726 (3P5)_PCR/7776 [Gene]SCO4726/7081 NC_003888 SC6G4.04, rpmJ, 50S ribosomal protein L36, len: 37 aa; highly similar to many e.g. RL36_BACST 50S ribosomal protein L36 (37 aa), fasta scores; opt: 225 z-score: 380.1 E() : 6.6e-14, 78.4% identity in 37 aa overlap. Contains PS0082 8 Ribosomal protein L36 signature and Pfam match to entry PF00444 L36, Ribosomal protein L36, score 70.40, E-value 3.1 e-22 4 1 20 22 [Reporter]SCO1750 (1P20)_PCR/1951 [Gene]SCO1750/1782 NC_003888 2SCI34.03c, probable acyl CoA dehydrogenase, len: 397 aa; similar to many dehydrogenases, e.g. SW:GCDH_HUMAN (EMBL:U69141) Homo sapiens (Human) glutaryl-CoA dehydrogenase, mitochondrial precursor (EC 1.3.99.7) GcdH, 438 aa; fasta scores: opt: 1208 z-score: 1358.9 E(): 0; 48.2% identity in 398 aa overlap. Contains 2x Pfam matches to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase 4 4 10 19 [Reporter]SCO6617 (4L5)_PCR/7775 [Gene]SCO6617/7080 NC_003888 SC1F2.14c, unknown, len: 109 aa 4 1 20 21 [Reporter]SCO2949 (2L20)_PCR/1950 [Gene]SCO2949/1781 NC_003888 SCE59.08, murA, UDP-N-acetylglucosamine transferase, len: 448 aa; identical to TR:BAA85335 (EMBL:AB033486) Streptomyces lividans UDP-N-acetylglucosamine transferase MurA, 446 aa and similar to SW:MURA_ECOLI (EMBL:M92358) Escherichia coli UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7) MurA, 419 aa; fasta scores: opt: 660 z-score: 763.5 E(): 0; 35.7% identity in 437 aa overlap 4 1 20 20 [Reporter]SCO7378 (3H20)_PCR/1949 [Gene]SCO7378/1780 NC_003888 SC10G8.05c, unknown, len: 236 aa. 1 3 15 10 [Reporter]SCO3030 (13I2)_PCR/4349 [Gene]SCO3030/3959 NC_003888 SCE34.11c, hypothetical protein, len: 140 aa; similar to TR:O53351 (EMBL:AL021840) Mycobacterium tuberculosis hypothetical 16.8 kD protein MTV015.30c, 163 aa; fasta scores: opt: 353 z-score: 430.4 E(): 1.6e-16; 48.3% identity in 147 aa overlap 1 3 15 9 [Reporter]SCO0256 (14E2)_PCR/4348 [Gene]SCO0256/3958 NC_003888 SCF20.02, possible short chain oxidoreductase, len: 265aa; similar to many oxidoreductases e.g. TR:Q9ZNN8 (EMBL:AB009078) L-2,3-butanediol dehydrogenase from Corynebacterium glutamicum (258 aa) fasta scores; opt: 784, z-score: 848.0, E(): 0, (49.4% identity in 255 aa overlap). Contains Pfam matches to entry PF00106 adh_short, short chain dehydrogenase and to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature 1 3 15 8 [Reporter]SCO0150 (15A2)_PCR/4347 [Gene]SCO0150/3957 NC_003888 SCJ33.14, unknown, len: 164 aa 1 3 15 7 [Reporter]SCO1027 (15M22)_PCR/4346 [Gene]SCO1027/3956 NC_003888 SCG20A.07, hypothetical protein, len: 157 aa; similar to SW:Y0BR_MYCTU (EMBL:Z77724) Mycobacterium tuberculosis hypothetical 19.0 kD protein CY227.27c, 167 aa; fasta scores: opt: 218 z-score: 283.7 E(): 2.5e-08; 30.7% identity in 150 aa overlap 1 3 15 6 [Reporter]SCO0917 (16I22)_PCR/4345 [Gene]SCO0917/3955 NC_003888 SCM10.05, possible oxygenase, len: 339 aa; similar to TR:Q9X2P4 (EMBL:AF041061) Mycobacterium smegmatis alkanal monooxygenase-like hypothetical protein AmlP, 289 aa; fasta scores: opt: 269 z-score: 305.4 E(): 1.3e-09; 29.2% identity in 291 aa overlap and to SW:LUXA_VIBHA (EMBL:M10961) Vibrio harveyi alkanal monooxygenase alpha chain (EC 1.14.14.3) LuxA, 355 aa; fasta scores: opt: 208 z-score: 236.6 E(): 8.6e-06; 27.8% identity in 205 aa overlap. Contains Pfam match to entry PF00296 bac_luciferase, Bacterial luciferase 1 3 15 5 [Reporter]SCO5176 (17E22)_PCR/4344 [Gene]SCO5176/3954 NC_003888 SCP8.39, probable reductase, len: 370 aa; similar to TR:O54256 (EMBL:AJ224512) Streptomyces nogalater putative dTDP-4-keto-6-deoxyhexose reductase SnoG, 328 aa; fasta scores: opt: 316 z-score: 337.4 E(): 2.5e-11; 30.5% identity in 364 aa overlap 4 4 10 18 [Reporter]SCO5718 (5H5)_PCR/7774 [Gene]SCO5718/7079 NC_003888 SC3C3.04, putative secreted protein, len: 524 aa; may contain N-terminal signal sequence with long leader 1 3 15 4 [Reporter]SCO2884 (18A22)_PCR/4343 [Gene]SCO2884/3953 NC_003888 SCE6.21, possible cytochrome P450, len: 408 aa. Highly similar to many other cytochrome P450 proteins including: Saccharopolyspora erythraea (Streptomyces erythraeus) SW:CPXJ_SACER(EMBL:X60379) 6-deoxyerythronolide B hydroxylase (404 aa), fasta scores opt: 777 z-score: 888.8 E():0 37.8% identity in 410 aa overlap and Streptomyces fradiae TR:Q9XCC6(EMBL:AF145049) (433 aa), fasta scores opt: 1253 z-score: 1430.3 E():0 50.9% identity in 409 aa overlap. Also similar to the adjoining CDS SCE6.20 (410 aa), fasta scores opt: 257 z-score: 252.4 E(): 1.5e-08 26.5% identity in 407 aa overlap. Contains a PS00086 Cytochrome P450 cysteine heme-iron ligand signature and a Pfam match to entry PF00067 p450, Cytochrome P450. 4 4 10 17 [Reporter]SCO2034 (6D5)_PCR/7773 [Gene]SCO2034/7078 NC_003888 SC4G6.03c, possible prolipoprotein diacyleglyceryl transferase, len: 343aa; similar to many eg. SW:LGT_STAAU prolipoprotein diacyleglyceryl transferase from Staphylococcus aureus (279 aa) fasta scores; opt: 434, z-score: 465.7, E(): 1.3e-18, (32.4% identity in 250 aa overlap). Also similar to TR:O06131 (EMBL:Z95554) hypothetical protein from Mycobacterium tuberculosis (468 aa) fasta scores; opt: 735, z-score: 778.7, E(): 0, (46.9% identity in 384 aa overlap). Contains possible hydrophobic membrane spanning regions 4 1 20 19 [Reporter]SCO5623 (4D20)_PCR/1948 [Gene]SCO5623/1779 NC_003888 SC2E1.40c, probable secreted protein, len: 226 aa; some similarity to two putative secreted M. tuberculosis proteins YX22_MYCTU MTCY274.22 (249 aa), fasta scores; opt: 315 z-score: 268.8 E(): 9.7e-08, 40.7% identity in 145 aa overlap (also upstream of rpsB) and Y0GK_MYCTU MTCY13D12.20 (407 aa), fasta scores; opt: 218 z-score: 176.1 E(): 0.014, 34.1% identity in 164 aa overlap. Contains probable N-terminal signal sequence. 1 3 15 3 [Reporter]SCP1.269 (19M18)_PCR/4342 [Gene]SCP1.269/3952 NC_003888 SCP1.269, possible secreted protein, len: 123aa; contains a possible cleavable N-terminal signal sequence. 4 4 10 16 [Reporter]SCO3601 (7P1)_PCR/7772 [Gene]SCO3601/7077 NC_003888 SC66T3.12, conserved hypothetical protein, len: 161 aa; unknown function, similar to hypothetical proteins from several organisms, often adjacent to a putative ion transport protein e.g. TR:O05882 (EMBL:Z95121) Mycobacterium tuberculosis hypothetical protein (160 aa), fasta scores; opt: 348 z-score: 435.7 E(): 6.1e-17, 44.1% identity in 152 aa overlap. Similar to TR:O69959 (EMBL:AL022268) S.coelicolor hypothetical protein (161 aa) (68.9% identity in 161 aa overlap), which is also adjacent to a possible ion transport protein 4 1 20 18 [Reporter]SCO6694 (5P16)_PCR/1947 [Gene]SCO6694/1778 NC_003888 SC4C6.04, possible transcriptional regulator, len: 204 aa; similar to many e.g. TR:P72185 (EMBL:U51164), HemR, Propionibacterium freudenreichii repressor protein from hemYHBXRL gene cluster (involved in the biosynthetic pathway from glutamate to protoheme) (259 aa), fasta scores; opt: 205 z-score: 251.7 E(): 1.1e-06, 28.5% identity in 200 aa overlap. Contains probable helix-turn-helix motif at aa 45-66 (Score 1187, +3.23 SD). Weakly similar to others from S.coelicolor e.g. TR:O69987 (EMBL:AL022268) S.coelicolor probable transcriptional regulator, partial CDS (>111 aa) (42.4% identity in 85 aa overlap) is similar to the N-terminal half 1 3 15 2 [Reporter]SCO2810 (20E18)_PCR/4341 [Gene]SCO2810/3951 NC_003888 SCBAC17F8.01, unknown, len: 220aa: no significant database matches.,2SCC13.18, unknown (fragment), len: >268 aa 4 4 10 15 [Reporter]SCO5561 (8L1)_PCR/7771 [Gene]SCO5561/7076 NC_003888 SC7A1.05c, conserved hypothetical protein, len: 77 aa; similar to several putative /hypotheticals eg. TR:O28868 (EMBL:AE001007) proposed transcriptional regulatory protein from Archaeoglobus fulgidus (77 aa) fasta scores; opt: 123, z-score: 217.3, E(): 8.3e-05, (31.9% identity in 69 aa overlap). 4 1 20 17 [Reporter]SCO2180 (6L16)_PCR/1946 [Gene]SCO2180/1777 NC_003888 SC5F7.21, pdhL, probable dihydrolipoamide dehydrogenase, len: 486 aa; note possible downstream translational start sites. Similar to many prokaryote and eukaryote egs. TR:Q9Z6I5 (EMBL: AF047034) dihydrolipoamide dehydrogenase from Streptomyces seoulensis (462 aa) fasta scores; opt: 2780, z-score: 3088.0, E(): 0, (90.3% identity in 462 aa overlap), TR:Q54101 (EMBL:L38646) NADH ferredoxin oxidoreductase from Saccharopolyspora erythraea (456 aa) fasta scores; opt: 1918, z-score: 2131.8, E(): 0, (64.2% identity in 452 aa overlap) and SW:DLDH_PEA dihydrolipoamide dehydrogenase from Pisum sativum (Garden pea) (501 aa) fasta scores; opt: 1162, z-score: 1292.6, E(): 0, (41.8% identity in 466 aa overlap). Contains Pfam match to entry PF00070 pyr_redox, Pyridine nucleotide-disulphide oxidoreductase class-I and Prosite match to PS00076 Pyridine nucleotide-disulphide oxidoreductases class-I active site. 1 3 14 22 [Reporter]SCO0989 (1E14)_PCR/4339 [Gene]SCO0989/3950 NC_003888 2SCG2.02c, unknown, len: 134 aa 4 4 10 14 [Reporter]SCO6602 (9H1)_PCR/7770 [Gene]SCO6602/7075 NC_003888 SC8A6.23, probable transmembrane sugar transport pro tein, len: 342 aa; similar to e.g. S. coelicolor TR:P72398 (EMBL:Y07706) putative maltose permease malF (334 aa), fast a scores; opt: 494 z-score: 521.6 E(): 8.2e-22, 32.6% ident ity in 322 aa overlap. Contains PS00017 ATP/GTP-binding sit e motif A (P-loop), PS00402 Binding-protein-dependent trans port systems inner membrane comp signature and Pfam match t o entry PF00528 BPD_transp, Binding-protein-dependent trans port systems inner membrane component, score 42.00, E-value 1.3e-08 4 1 20 16 [Reporter]SCO2659 (7H16)_PCR/1945 [Gene]SCO2659/1776 NC_003888 SC6D10.02, possible sugar transport system permease protein, len: 314 aa; similar to TR:Q9X6C4 (EMBL:AF135398) Thermus brockianus putative integral membrane protein BdtF, 280 aa; fasta scores: opt: 498 z-score: 582.4 E(): 5e-25; 35.4% identity in 288 aa overlap and to SW:LACF_AGRRD (EMBL:X66596) Agrobacterium radiobacter lactose transport system permease protein LacF, 298 aa; fasta scores: opt: 437 z-score: 511.6 E(): 4.4e-21; 31.1% identity in 315 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible N-terminal region signal peptide sequence and possible hydrophobic membrane spanning regions 4 4 10 13 [Reporter]SCO6477 (10D1)_PCR/7769 [Gene]SCO6477/7074 NC_003888 SC9C7.13c, unknown, len: 179 aa; similar to TR:O32075 (EMBL:Z99119) hypothetical protein from Bacillus subtilis (173 aa) fasta scores; opt: 261, z-score: 298.5, E(): 2.4e-09, (32.9% identity in 170 aa overlap). Contains a region of weak similarity to Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 4 1 20 15 [Reporter]SCO5238 (8D16)_PCR/1944 [Gene]SCO5238/1775 NC_003888 SC7E4.35, possible TetR-family protein, len: 214 aa; similar to TR:CAB69777 (EMBL:AL137187) Streptomyces coelicolor putative tetR-family transcriptional regulator SC7A8.28, 194 aa; fasta scores: opt: 326 z-score: 399.8 E(): 8.5e-15; 34.9% identity in 192 aa overlap and to TR:CAB93063 (EMBL:AL357432) Streptomyces coelicolor putative transcriptional regulator SCD95A.36c, 202 aa; fasta scores: opt: 458 z-score: 451.3 E(): 1.5e-19; 41.8% identity in 194 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 4 4 10 12 [Reporter]SCO2757 (10P21)_PCR/7768 [Gene]SCO2757/7073 NC_003888 SCC57A.28, possible LysR-family transcriptional regulator, len: 313 aa. Similar to several other regulators including: Escherichia coli SW:XAPR_ECOLI (EMBL:X63976) xanthosine operon regulatory protein (294 aa), fasta scores opt: 322 z-score: 366.5 E(): 5e-13 27.4% identity in 292 aa overlap. Also similar to several other putative Streptomyces coelicolor regulators including: TR:O86805 (EMBL:AL031317) SC6G4.44 (306 aa), fasta scores opt: 523 z-score: 590.0 E(): 1.8e-25 37.2% identity in 320 aa overlap and TR:CAB62680 (EMBL:AL133422) SCM1.22C (301 aa), fasta scores opt: 636 z-score: 715.9 E(): 1.8e-32 41.5% identity in 294 aa overlap. Contains a Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family, with the putative helix-turn-helix motif situated between residues 17..38 (+4.21 SD). 4 1 20 14 [Reporter]SCO5409 (9P12)_PCR/1943 [Gene]SCO5409/1774 NC_003888 SC8F4.13, putative membrane protein, len: 114 aa; similar to TR:O31485 (EMBL:Z99106) hypothetical protein from Bacillus subtilis (96 aa) fasta scores; opt: 179, z-score: 259.7, E(): 4.7e-07, 37.4% identity in 91 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 10 11 [Reporter]SCO4670 (11L21)_PCR/7767 [Gene]SCO4670/7072 NC_003888 SCD840A.16c, probable serine protease precursor, len: 463 aa; similar to SW:PRTC_STRGR (EMBL:L29018) Streptomyces griseus serine protease C precursor (EC 3.4.21.-) SprC, 457 aa; fasta scores: opt: 1761 z-score: 1679.7 E(): 0; 59.4% identity in 458 aa overlap and to TR:Q9XA96 (EMBL:AL096837) Streptomyces coelicolor putative serine protease SCF43A.19, 458 aa; fasta scores: opt: 1637 z-score: 1264.0 E(): 0; 55.9% identity in 460 aa overlap. Contains Pfam match to entry PF00089 trypsin, Trypsin and matches to Prosite entries PS00135 Serine proteases, trypsin family, serine active site and PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Contains also possible N-terminal region signal peptide sequence 4 1 20 13 [Reporter]SCO2589 (10L12)_PCR/1942 [Gene]SCO2589/1773 NC_003888 SCC123.27, possible glycosyl transferase, len: 743 aa. Weakly similar to Staphylococcus epidermidis TR:AAD56434(EMBL:AF162863) poly(glycerolphosphate) glycerophosphotransferase, TagF (721 aa), fasta scores opt: 564 z-score: 661.8 E(): 1.8e-29 27.4% identity in 731 aa overlap and Bacillus subtilis SW:TAGF_BACSU(EMBL:X15200) teichoic acid biosynthesis protein, TagF (746 aa), fasta scores opt: 567 z-score: 665.1 E(): 1.2e-29 30.9% identity in 417 aa overlap. Also similar to the adjoining gene fragment, CDS SCC123.28 (> 97 aa), fasta scores opt: 298 z-score: 353.4 E(): 2.9e-14 47.9% identity in 96 aa overlap. Contains a Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases 4 4 10 10 [Reporter]SCO4317 (12H21)_PCR/7766 [Gene]SCO4317/7071 NC_003888 SCD95A.50, unknown, len: 73 aa 4 1 20 12 [Reporter]SCO2344 (11H12)_PCR/1941 [Gene]SCO2344/1772 NC_003888 SCC8A.02c, possible integral membrane transport protein, len: 689aa; similar to many eg. TR:CAB89031 (EMBL:AL353870) putative membrane transport protein from Streptomyces coelicolor (714 aa) fasta scores; opt: 1767, z-score: 1907.0, E(): 0, 46.1% identity in 670 aa overlap and TR:P96712 (EMBL:D50098) multidrug transporter from Bacillus subtilis (512 aa) fasta scores; opt: 1168, z-score: 1262.8, E(): 0, 37.6% identity in 495 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and possible membrane-spanning hydrophobic regions. 4 4 10 9 [Reporter]SCO4868 (13D21)_PCR/7765 [Gene]SCO4868/7070 NC_003888 SCK20.09c, possible lipoprotein, len: 281 aa; similar to TR:CAC14938 (EMBL:AL449216) Streptomyces coelicolor putative secreted protein 3SC5B7.14c, 231 aa; fasta scores: opt: 330 z-score: 353.2 E(): 3.9e-12; 35.3% identity in 218 aa overlap. Contains Pfam match to entry PF01471 PG_binding_1, Putative peptidoglycan binding domain and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 4 1 20 11 [Reporter]SCO4186 (12D12)_PCR/1940 [Gene]SCO4186/1771 NC_003888 SCD66.23, conserved hypothetical protein, len: 418 aa; highly similar to TR:Q54238 (EMBL:D63706;) Streptomyces griseus hypothetical protein ORF5, 416 aa; fasta scores: opt: 1720 z-score: 2020.0 E(): 0; 62.6% identity in 414 aa overlap 4 1 20 10 [Reporter]SCO3101 (13P8)_PCR/1939 [Gene]SCO3101/1770 NC_003888 SCE41.10c, possible lipoprotein, len: 215 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 1 3 14 21 [Reporter]SCO0561 (2A14)_PCR/4338 [Gene]SCO0561/3949 NC_003888 SCF73.08c, furS, Fe regulatory protein, len: 151 aa; identical to TR:CAB38251 (EMBL:AJ132989) S. coelicolor Fe regulatory protein (151 aa), highly similar to e.g. FUR_MYCTU ferric uptake regulation protein (147 aa), fasta scores; opt: 613 z-score: 742.2 E(): 0, 64.8% identity in 142 aa overlap. Contains Pfam match to entry PF01475 FUR, Ferric uptake regulator family 1 3 14 20 [Reporter]SCO5908 (3M10)_PCR/4337 [Gene]SCO5908/3948 NC_003888 SC10A5.13, unknown, len: 64 aa 1 3 14 19 [Reporter]SCO6944 (4I10)_PCR/4336 [Gene]SCO6944/3947 NC_003888 SC1G8.16c, conserved hypothetical protein, len: 633 aa. Highly similar to several proteins of undefined function including: Streptomyces coelicolor TR:CAB52841 (EMBL:AL109848) SCI51.17 (612 aa), fasta scores opt: 2658 z-score: 3108.3 E():0 64.4% identity in 590 aa overlap and Schizosaccharomyces pombe (Fission yeast) SW:YAY3_SCHPO (EMBL:Z69380) SPAC4H3.03C (649 aa), fasta scores opt: 943 z-score: 1101.2 E():0 32.8% identity in 640 aa overlap. 1 3 14 18 [Reporter]SCO5445 (5E10)_PCR/4335 [Gene]SCO5445/3946 NC_003888 SC3D11.02c, hypothetical protein, len: 188aa; previously sequenced as TR:Q9X945 (EMBL:AJ001205) and predicted as a 134 aa protein. Also similar to TR:O06198 (EMBL:Z95387) hypothetical protein from Mycobacterium tuberculosis (166 aa) fasta scores; opt: 407, z-score: 496.4, E(): 3.1e-20, 44.0% identity in 159 aa overlap 1 3 14 17 [Reporter]SCO5784 (6A10)_PCR/4334 [Gene]SCO5784/3945 NC_003888 SC4H2.05, probable two-component sensor protein, len: 358 aa; similar to many e.g. DEGS_BACSU P13799 sensor protein DegS (ec 2.7.3.-), Bacillus subtilis (385 aa), fasta scores; opt: 317 z-score: 334.6 E(): 2e-11, 31.3% identity in 227 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 14 16 [Reporter]SCO6754 (7M6)_PCR/4333 [Gene]SCO6754/3944 NC_003888 SC6A5.03, possible glycerol dehydrogenase, len: 340aa; similar to many eg. SW:GLDA_PSEPU glycerol dehydrogenase, GldA, from Pseudomonas putida (365 aa) fasta scores; opt: 261, z-score: 303.7, E(): 1.3e-09, (27.9% identity in 308 aa overlap). 4 4 10 8 [Reporter]SCO0707 (14P17)_PCR/7764 [Gene]SCO0707/7069 NC_003888 SCF42.17c, possible branched-chain amino acid ABC transport permease, len: 398 aa. Similar to many amino acid transporters including: Pseudomonas aeruginosa SW:BRAE_PSEAE (EMBL:D90223) high-affinity branched-chain amino acid transport protein BraE (417 aa), fasta scores opt: 327 z-score: 334.2 E(): 3.2e-11 28.3% identity in 336 aa overlap and Streptomyces coelicolor TR:CAB52066 (EMBL:AL109732) putative branched chain amino acid transport permease SC7H2.24 (608 aa), fasta scores opt: 403 z-score: 407.6 E(): 2.6e-15 31.0% identity in 342 aa overlap. Contains a possible N-terminal signal sequence and multiple possible membrane spanning hydrophobic domains. 1 3 14 15 [Reporter]SCO7250 (8I6)_PCR/4332 [Gene]SCO7250/3943 NC_003888 SC7A12.17c, unknown, len: 639 aa. Highly similar to another hypothetical protein: Streptomyces coelicolor TR:Q9S2M7(EMBL:AL109661) SC6E10.10 (943 aa), fasta scores opt: 1245 z-score: 1258.0 E(): 0 42.5% identity in 664 aa overlap. Contains a Pfam match to entry PF01510 Amidase_2, N-acetylmuramoyl-L-alanine amidase. 4 4 10 7 [Reporter]SCO0277 (15L17)_PCR/7763 [Gene]SCO0277/7068 NC_003888 SCF85.05c, unknown, len:222 aa. Low similarity to Haemophilus influenzae SW:PEPE_HAEIN (EMBL; U32740) peptidase E (EC 3.4.-.-) (alpha-aspartyl dipeptidase) (226 aa), fasta scores opt: 134 z-score: 169.6 E(): 0.043 24.4% identity in 209 aa overlap. 4 1 20 9 [Reporter]SCO0433 (14L8)_PCR/1938 [Gene]SCO0433/1769 NC_003888 SCF51A.11, possible transcriptional regulator, len: 334 aa. Similar to Streptomyces coelicolor TR:Q9ZBT5 (EMBL; AL034446) putative regulatory protein SC1A9.17C (327 aa), fasta scores opt: 494 z-score: 573.8 E(): 1.3e-24 36.9% identity in 333 aa overlap. Contains a possible helix-turn-helix motif between residues 290..311 (+2.71 SD). 1 3 14 14 [Reporter]SCO7732 (9E6)_PCR/4331 [Gene]SCO7732/3942 NC_003888 SC8D11.23, unknown, len: 128 aa 4 4 10 6 [Reporter]SCO3845 (16H17)_PCR/7762 [Gene]SCO3845/7067 NC_003888 SCH69.15, possible protein phosphatase, len: 515aa; similar to many of undefined function egs. TR:P71588 (EMBL:Z80233) hypothetical protein from Mycobacterium tuberculosis (514 aa) fasta scores; opt: 989, z-score: 916.0, E(): 0, (42.9% identity in 536 aa overlap) and TR:Q50188 (EMBL:Z70722) putative phophoprotein phosphatase from Mycobacterium leprae (509 aa) fasta scores; opt: 966, z-score: 895.0, E(): 0, (40.0% identity in 530 aa overlap). Contains 2 Pfam matches to entry PF00481 PP2C, Protein phosphatase 2C. could have a role in signalling. 4 1 20 8 [Reporter]SCO0558 (15H8)_PCR/1937 [Gene]SCO0558/1768 NC_003888 SCF73.05c, unknown, len: 88 aa 1 3 14 13 [Reporter]SCO5713 (10A6)_PCR/4330 [Gene]SCO5713/3941 NC_003888 SC9F2.03, possible peptide transport ATP-binding protein, len: 393aa; similar to many (confirmed and proposed) eg. SW:OPPD_ECOLI oligopeptide transport ATP-binding protein from Escherichia coli (337 aa) fasta scores; opt: 870, z-score: 693.2, E(): 2.7e-31, (44.1% identity in 311 aa overlap).Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also similar to the gene immediately downstream (SC9F2.04) suggesting a local duplication event fasta scores; opt: 722, z-score: 477.4, E(): 9.4e-22, (42.8% identity in 327 aa overlap). Also contains Pfam match to entry PF00005 ABC_tran, ABC transporter, score 197.30, E-value 2.3e-55. 4 4 10 5 [Reporter]SCO3403 (17D17)_PCR/7761 [Gene]SCO3403/7066 NC_003888 SCE9.10c, folE, probable GTP cyclohydrolase I, len: 201 aa; similar to many e.g. SW:GCH1_BACSU (EMBL:M37320), MtrA, Bacillus subtilis GTP cyclohydrolase I (190 aa), fasta scores; opt: 728 z-score: 880.0 E(): 0, 58.9% identity in 185 aa overlap. Contains Pfam match to entry PF01227 GTP_cyclohydroI, GTP cyclohydrolase I, score 351.80, E-value 7.2e-102, PS00859 GTP cyclohydrolase I signature 1 and PS00860 GTP cyclohydrolase I signature 2 4 1 20 7 [Reporter]SCO1553 (16D8)_PCR/1936 [Gene]SCO1553/1767 NC_003888 SCL11.09c, probable uroporphyrin-III methyltransferase, len: 410 aa; similar to TR:O05812 (EMBL:Z95207) Mycobacterium tuberculosis CysG, 405 aa; fasta scores: opt: 1487 z-score: 1630.7 E(): 0; 58.7% identity in 409 aa overlap and to SW:SUMT_BACME (EMBL:M62881) Bacillus megaterium uroporphyrin-III C-methyltransferase (EC 2.1.1.107) CobA, 238 aa; fasta scores: opt: 719 z-score: 794.9 E(): 0; 48.1% identity in 233 aa overlap. Contains Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases and match to Prosite entry PS00840 Uroporphyrin-III C-methyltransferase signature 2 1 3 14 11 [Reporter]SCO4116 (12I2)_PCR/4328 [Gene]SCO4116/3940 NC_003888 SCD72A.02, possible AfsR-like regulatory protein, len: 1114 aa; similar to many from actinomycetes e.g. TR:CAB61705 (EMBL:AL133220) putative multi-domain regulatory protein from Streptomyces coelicolor (1334 aa) fasta scores; opt: 1324, z-score: 1364.5, E(): 0, 41.1% identity in 1357 aa overlap and SW:P25941 (AFSR_STRCO) regulatory protein AfsR from Streptomyces coelicolor (993 aa) fasta scores; opt: 533, z-score: 548.6, E(): 4.3e-23, 29.3% identity in 1014 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 4 10 4 [Reporter]SCP1.129 (18P13)_PCR/7760 [Gene]SCP1.129/7065 NC_003888 SCP1.129, unknown, len: 145aa; 4 1 20 6 [Reporter]SCO0489 (17P4)_PCR/1935 [Gene]SCO0489/1766 NC_003888 SCF34.08c, conserved hypothetical protein, len: 70 aa; unknown function, similar to hypothetical proteins from several bacteria e.g. SW:MBTH_MYCTU (EMBL:Z95208), MbtH, Mycobacterium tuberculosis hypothetical protein (71 aa), fasta scores; opt: 329 z-score: 486.3 E(): 9.9e-20, 70.3% identity in 64 aa overlap. Also similar to TR:Q9Z388 (EMBL:AL035654) Streptomyces coelicolor small conserved hypothetical protein (71 aa) (74.2% identity in 62 aa overlap) 4 4 10 3 [Reporter]SCP1.257 (19L13)_PCR/7759 [Gene]SCP1.257/7064 NC_003888 SCP1.257, unknown, hydrophilic, len: 137aa; 4 1 20 5 [Reporter]SCO2986 (18L4)_PCR/1934 [Gene]SCO2986/1765 NC_003888 SCE50.14c, hypothetical protein, len: 137 aa; similar to TR:CAB62728 (EMBL:AL133423) Streptomyces coelicolor hypothetical 14.2 kD protein SC4A7.24c, 139 aa; fasta scores: opt: 533 z-score: 620.7 E(): 4e-27; 60.9% identity in 133 aa overlap 4 4 10 2 [Reporter]SCO5997 (20D13)_PCR/7758 [Gene]SCO5997/7063 NC_003888 SCBAC16H6.32, possible secreted protein, len: 172aa: similar to others from Streptomyces coelicolor eg. TR:Q9AJX2 (EMBL:AL513407) putative secreted protein SCI33.04 (244 aa) fasta scores; opt: 622, Z-score: 669.7, 61.111% identity (62.658% ungapped) in 162 aa overlap. Contains a possible N-terminal signal sequence 4 1 20 4 [Reporter]SCP1.99 (19H4)_PCR/1933 [Gene]SCP1.99/1764 NC_003888 SCP1.99, unknown, len: 163aa; 4 4 9 22 [Reporter]SCO5265 (1D9)_PCR/7756 [Gene]SCO5265/7062 NC_003888 2SC7G11.27c, hypothetical protein, len: 333 aa; low similarity to TR:O32200 (EMBL:Z99120) Bacillus subtilis YvqG protein, 240 aa; fasta scores: opt: 172 z-score: 193.3 E(): 0.0027; 25.0% identity in 244 aa overlap 4 1 20 3 [Reporter]SCO4905 (20D4)_PCR/1932 [Gene]SCO4905/1763 NC_003888 2SCK8.31c, possible lipoprotein, len: 203 aa; similar to TR:Q9K453 (EMBL:AL359215) Streptomyces coelicolor putative secreted protein SC2H12.31, 211 aa; fasta scores: opt: 142 z-score: 175.4 E(): 0.033; 29.3% identity in 205 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 4 9 21 [Reporter]SCO2619 (2P5)_PCR/7755 [Gene]SCO2619/7061 NC_003888 SCC80.04c, clpP1, ATP dependent Clp protease proteolytic subunit 1, len: 219 aa; identical to previously sequenced TR:Q9ZH59 (EMBL:AF071885) Streptomyces coelicolor ATP dependent Clp protease proteolytic subunit 1 (EC 3.4.21.92) ClpP1, 218 aa. Contains Pfam match to entry PF00574 CLP_protease, Clp protease 4 1 20 2 [Reporter]SCO2816 (20L24)_PCR/1931 [Gene]SCO2816/1762 NC_003888 SCBAC17F8.07, conserved hypothetical protein, len: 366 aa; similar to many e.g. TR:Q9I0Z9 (EMBL:AE004676) hypothetical protein from Pseudomonas aeruginosa (333 aa) fasta scores; opt: 588, Z-score: 637.0, 47.826% identity (50.305% ungapped)in 345 aa overlap 4 4 9 20 [Reporter]SCO4710 (3L5)_PCR/7754 [Gene]SCO4710/7060 NC_003888 SCD31.35, rpmC, 50S ribosomal protein L29, len: 74 aa; highly similar to SW:RL29_BACST (PIR:A02825) Bacillus stearothermophilus 50S ribosomal protein L29 RpmC, 66 aa; fasta scores: opt: 244 z-score: 361.6 E(): 1e-12; 63.9% identity in 61 aa overlap. Contains Pfam match to entry PF00831 Ribosomal_L29, Ribosomal L29 protein and match to Prosite entry PS00323 Ribosomal protein S19 signature 4 1 19 22 [Reporter]SCO1357 (1L20)_PCR/1929 [Gene]SCO1357/1761 NC_003888 2SCG61.39, hypothetical protein, len: 153 aa; similar to TR:O53240 (EMBL:AL021287) hypothetical protein from Mycobacterium tuberculosis (163 aa) fasta scores; opt: 266, z-score: 354.4, E(): 2.9e-12, 34.8% identity in 155 aa overlap. 4 1 19 21 [Reporter]SCO6506 (2H20)_PCR/1928 [Gene]SCO6506/1760 NC_003888 SC1E6.15, gvpL, probable gas vesicle protein, len: 504 aa; simialar to e.g. GVL1_HALSA GVPL protein, plasmid (281 aa), fasta scores; opt: 111 z-score: 229.0 E(): 1.8e-05, 24.2% identity in 264 aa overlap 1 3 14 10 [Reporter]SCO2863 (13E2)_PCR/4327 [Gene]SCO2863/3939 NC_003888 SCE20.37, possible helicase, len: 945 aa. Weakly similar to Saccharomyces cerevisiae (Baker's yeast) TR:Q06683(EMBL:U32517) DEAD box family helicase family protein (689 aa), fasta scores opt: 455 z-score: 511.5 E(): 4.1e-21 27.2% identity in 357 aa overlap. Also weakly similar to several bacteriophage proteins including: Lactococcus bacteriophage (isolate 7-9) SW:V51K_BPL79 (EMBL:M36388) 51.5 KD protein which may play a role in either regulating bacteriophage replication or bacteriophage specific genes (452 aa), fasta scores opt: 407 z-score: 460.0 E(): 3.1e-18 27.4% identity in 328 aa overlap. Contains Prosite hit to 2xPS00017 ATP/GTP-binding site motif A (P-loop) and a Pfam match to entry PF00271 helicase_C, Helicases conserved C-terminal domain. 1 3 14 9 [Reporter]SCO0399 (14A2)_PCR/4326 [Gene]SCO0399/3938 NC_003888 SCF62.25, possible membrane protein, len: 407 aa. Weakly similar to a several e.g. Erwinia amylovora SW: AMSJ_ERWAM (EMBL; X77921) amylovoran biosynthesis protein AmsJ (415 aa), fasta scores opt: 107 z-score: 129.1 E(): 7.8 21.3% identity in 423 aa overlap and Escherichia coli SW:WCAK_ECOLI (EMBL; U38473) colanic acid biosynthesis protein WcaK (426 aa), fasta scores opt: 111 z-score: 133.6 E():4.3 20.1% identity in 398 aa overlap. Contains a possible N-terminal signal sequence and a membrane spanning hydrophobic domain. Also contains a TTA leucine codon, possible target for bldA regulation and a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 14 8 [Reporter]SCO1845 (14M22)_PCR/4325 [Gene]SCO1845/3937 NC_003888 SCI8.30, possible low-affinity phosphate transport protein, len: 423 aa. Highly similar to many possible transporters, including: Streptomyces halstedii SW: PITH_STRHA (EMBL: L05390) putative low-affinity inorganic phosphate transporter (fragment) (>213 aa), fasta scores opt: 505 z-score: 517.8 E(): 1.8e-21 45.9% identity in 194 aa overlap and Mycobacterium tuberculosis TR:O06411 (EMBL: Z95558) hypothetical 42.7 KD protein (417 aa), fasta scores opt: 1286 z-score: 1295.4 E():0 50.8% identity in 421 aa overlap. Contains a Pfam match to entry PF01384 PHO4, Phosphate transporter family. Also contains a possible N-terminal signal sequence and multiple possible membrane spanning hydrophobic domains. 1 3 14 7 [Reporter]SCO0544 (15I22)_PCR/4324 [Gene]SCO0544/3936 NC_003888 SCF11.24, hypothetical secreted protein, len: 510 aa; similar to various hypothetical proteins, e.g. SW:YM26_MYCTU (EMBL:Z70692) Mycobacterium tuberculosis hypothetical 56.3 KD protein RV2226, 513 aa; fasta scores: opt: 867 z-score: 952.2 E(): 0; 39.0% identity in 515 aa overlap. Contains probable N-terminal signal sequence 1 3 14 6 [Reporter]SCO1886 (16E22)_PCR/4323 [Gene]SCO1886/3935 NC_003888 SCI7.04c, possible integral membrane transport protein, len: 298aa; similar to many eg. SW:YURM_BACSU hypothetical ABC transporter from Bacillus subtilis (300 aa) fasta scores; opt: 582, z-score: 650.2, E(): 6.9e-29, (31.7% identity in 293 aa overlap) and TR:O87858 (EMBL:AL031013) putative transmembrane sugar transport protein from Streptomyces coelicolor (279 aa) fasta scores; opt: 540, z-score: 604.3, E(): 2.5e-26, (34.7% identity in 277 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible membrane spanning hydrophobic regions. 1 3 14 5 [Reporter]SCO3559 (17A22)_PCR/4322 [Gene]SCO3559/3934 NC_003888 SCH5.22c, possible oxidoreductase, len: 274aa; similar to TR:O69630 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (256 aa) fasta scores; opt: 289, z-score: 335.7, E(): 2.1e-11, (39.3% identity in 270 aa overlap). Also contains a region of similarity to many fatty acid desaturases from plants eg. SW:STAD_RICCO Stearoyl-acyl-carrier-protein desaturase from Ricinus communis (castor bean) (396 aa) fasta scores; opt: 112, z-score: 133.1, E(): 4.1, (25.3% identity in 146 aa overlap). 4 4 9 19 [Reporter]SCO6926 (4H5)_PCR/7753 [Gene]SCO6926/7059 NC_003888 SC1B2.32, unknown, len: 274 aa. Weakly similar to several including: Streptomyces coelicolor TR:CAB53276 (EMBL:AL109972) putative DNA-binding protein, SCJ9A.15C (290 aa), fasta scores opt: 225 z-score: 274.7 E(): 7.7e-08 29.0% identity in 238 aa overlap. 1 3 14 4 [Reporter]SCP1.126 (18M18)_PCR/4321 [Gene]SCP1.126/3933 NC_003888 SCP1.126, unknown, len: 78aa; 4 4 9 18 [Reporter]SCO5663 (5D5)_PCR/7752 [Gene]SCO5663/7058 NC_003888 SC3A9.04c, probable integral membrane protein, len: 226 aa; has highly proline-rich N-terminus and two possible transmembrane domains in the C-terminus. Similar to downstream ORF SC3A9.03c, fasta scores; E(): 6.5e-06, 41.0% identity in 134 aa overlap 4 1 19 18 [Reporter]SCO7691 (5L16)_PCR/1925 [Gene]SCO7691/1759 NC_003888 SC4C2.26, possible lyase, len: 471 aa; C-terminal region similar to C-terminal region of SW:TRPE_HALVO (EMBL:M83788) Halobacterium volcanii anthranilate synthase component I(EC 4.1.3.27) TrpE, 523 aa; fasta scores: opt: 545 z-score: 624.2 E(): 3.1e-27; 38.7% identity in 300 aa overlap. Contains Pfam match to entry PF00425 chorismate_bind, chorismate binding enzyme 1 3 14 3 [Reporter]SCP1.264 (19I18)_PCR/4320 [Gene]SCP1.264/3932 NC_003888 SCP1.264, unknown, len: 460aa; 4 4 9 17 [Reporter]SCO5773 (6P1)_PCR/7751 [Gene]SCO5773/7057 NC_003888 SC4H8.12c, possible monooxygenase, len: 541 aa; weakly similar to many eg. TR:Q54171 (EMBL:X87093) putative oxygenase urdE from Streptomyces fradiae (495 aa), fasta scores; opt: 333 z-score: 428.9 E(): 1.1e-16, 30.4% identity in 539 aa overlap 4 1 19 17 [Reporter]SCO7786 (6H16)_PCR/1924 [Gene]SCO7786/1758 NC_003888 SC5E9.34c, unknown, len: 195 aa; regions of weak similarity to transposases eg. TR:Q9RBZ3 (EMBL:AF169828) from Pseudomonas syringae (pv. glycinea) (357 aa) fasta scores; opt: 158, z-score: 201.5, E(): 0.00093, 26.7% identity in 135 aa overlap. 1 3 14 2 [Reporter]SCO3992 (20A18)_PCR/4319 [Gene]SCO3992/3931 NC_003888 SCBAC25E3.29c, unknown, doubtful CDS, len: 96 aa: no significant database matches. 4 4 9 16 [Reporter]SCO3597 (7L1)_PCR/7750 [Gene]SCO3597/7056 NC_003888 SC66T3.08c, conserved hypothetical protein, len: 270 aa; unknown function, similar to hypothetical proteins from Mycobacterium tuberculosis, Mycobacterium leprae and Bacillus subtilis e.g. SW:Y03O_MYCTU (EMBL:Z75555) Mycobacterium tuberculosis hypothetical protein (273 aa), fasta scores; opt: 413 z-score: 428.5 E(): 1.5e-16, 31.7% identity in 268 aa overlap. Contains poly-glycine tract at N-terminus which is absent in the similar sequences 4 1 19 16 [Reporter]SCO6178 (7D16)_PCR/1923 [Gene]SCO6178/1757 NC_003888 SC6C5.14c, possible deacetylase (putative secreted protein), len: 260 aa; similar to many e.g. SW:NODB_RHILT NodB, chitooligosaccharide deacetylase, nodulation protein B, from Rhizobium leguminosarum (215 aa) fasta scores; opt: 321, z-score: 420.9, E(): 3.7e-16, (35.0% identity in 197 aa overlap). Contains possible N-terminal region signal peptide sequence 1 3 13 22 [Reporter]SCO1286 (1A14)_PCR/4317 [Gene]SCO1286/3930 NC_003888 2SCG18.33, probable oxidoreductase, len: 374 aa; similar to TR:P96072 (EMBL:U76606) Streptomyces viridifaciens isobutylamine N-hydroxylase (EC 1.14.-.-) (IBAH) VlmH, 378 aa; fasta scores: opt: 1020 z-score: 1177.8 E(): 0; 45.9% identity in 379 aa overlap 4 4 9 15 [Reporter]SCO2147 (8H1)_PCR/7749 [Gene]SCO2147/7055 NC_003888 SC6G10.20c, trpD1, phosphoribosylanthranilate transferase, len: 354 aa; previously sequenced therefore identical to TR:O68608 (EMBL:AF052077). Also similar to many others eg. SW:TRPD_MYCTU from Mycobacterium tuberculosis (370 aa) fasta scores; opt: 1091, z-score: 1205.4, E(): 0, (51.4% identity in 352 aa overlap). Contains Pfam match to entry PF00591 Glycos_transf_3, glycosyl transferase family. 4 1 19 14 [Reporter]SCO5405 (9L12)_PCR/1921 [Gene]SCO5405/1756 NC_003888 SC8F4.09c, probable transcriptional regulator, len: 158 aa; similar to many e.g. TR:Q9X5U1 (EMBL:AF127374) from Streptomyces lavendulae (163 aa) fasta scores; opt: 382, z-score: 481.8, E(): 2e-19, 43.4% identity in 152 aa overlap and SW:P42195 (PECS_ERWCH) regulatory protein PecS from Erwinia chrysanthemi fasta scores; opt: 172, z-score: 225.1, E(): 3.9e-05, 28.7% identity in 136 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family and helix-turn-helix motif Score 1270 (+3.51 SD) in ID at residue 60. 4 4 9 14 [Reporter]SCO6598 (9D1)_PCR/7748 [Gene]SCO6598/7054 NC_003888 SC8A6.19, probable transcriptional regulator, len: 246 aa; similar to many members of the lacI family, e.g. CCP A_BACSU glucose-resistance amylase regulator (334 aa), fast a scores; opt: 313 z-score: 569.6 E(): 1.7e-24, 31.2% identity in 317 aa overlap. Contains PS00356 Bacterial regulatory proteins, lacI family signature, helix-tur-helix motif at aa 7-28 (Score 2389, +7.32 SD) and Pfam matches to entry P F00532 Peripla_BP_like, Periplasmic binding proteins and LacI family, score 86.50, E-value 5.3e-22 and Pfam match to entry PF00356 lacI, Bacterial regulatory proteins, lacI family, score 43.00, E-value 1e-10 4 1 19 13 [Reporter]SCO6246 (10H12)_PCR/1920 [Gene]SCO6246/1755 NC_003888 SCAH10.11c, iclR, transcriptional regulator for glyoxylate bypass, len: 270 aa; highly similar to SW:ICLR_ECOLI (EMBL:M31761) Escherichia coli acetate operon repressor IclR, 274 aa; fasta scores: opt: 538 Z-score: 603.9 E(): 5.5e-26; 40.234% identity in 256 aa overlap and to SW:GYLR_STRCO (EMBL:X14188) Streptomyces coelicolor glycerol operon regulatory protein, 254 aa; fasta scores: opt: 333 z-score: 389.8 E(): 2.4e-14; 32.8% identity in 250 aa overlap. Contains match to Pfam entry PF01614 IclR, Bacterial transcriptional regulator and a possible helix-turn-helix motif at residues 39..60 (+2.51 SD) 4 4 9 13 [Reporter]SCO1480 (9P21)_PCR/7747 [Gene]SCO1480/7053 NC_003888 SC9C5.04c, conserved hypothetical protein, len: 107 aa; highly similar to TR:P96802 (EMBL:U75344) Mycobacterium smegmatis integration host factor MihF, 105 aa; fasta scores: opt: 378 z-score: 470.3 E(): 1e-1; 58.1% identity in 105 aa overlap 4 1 19 12 [Reporter]SCO2605 (11D12)_PCR/1919 [Gene]SCO2605/1754 NC_003888 SCC88.16, unknown, len: 233 aa 4 4 9 12 [Reporter]SCO2753 (10L21)_PCR/7746 [Gene]SCO2753/7052 NC_003888 SCC57A.24, possible LacI-family transcriptional regulator, len: 348 aa. Similar to many other putative Streptomyces coelicolor LacI-family regulators including: TR:Q9X862 (EMBL:AL049661) SCE134.07 (353 aa), fasta scores opt: 489 z-score: 554.3 E(): 1.8e-23 33.8% identity in 358 aa overlap and TR:O86795 (EMBL:AL031317) SC6G4.32 (355 aa), fasta scores opt: 460 z-score: 521.7 E(): 1.2e-21 37.1% identity in 353 aa overlap. Contains a Prosite hit to PS00356 Bacterial regulatory proteins, lacI family signature and Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family. A putative helix-turn-helix motif is situated between residues 3..24 (+5.80 SD). 4 1 19 11 [Reporter]SCO4641 (12P8)_PCR/1918 [Gene]SCO4641/1753 NC_003888 SCD82.12, possible transmembrane efflux protein, len: 490 aa; similar to SW:TCMA_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C resistance and export protein TcmA, 538 aa; fasta scores: opt: 952 z-score: 977.6 E(): 0; 33.5% identity in 466 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and possible hydrophobic membrane spanning regions 4 4 9 11 [Reporter]SCO4081 (11H21)_PCR/7745 [Gene]SCO4081/7051 NC_003888 SCD25.17, unknown, len: 160 aa. 4 1 19 10 [Reporter]SCO3097 (13L8)_PCR/1917 [Gene]SCO3097/1752 NC_003888 SCE41.06c, possible secreted protein, len: 244 aa; similar to TR:O86308 (EMBL:Z96935) Micrococcus luteus Rpf protein precursor, 220 aa; fasta scores: opt: 366 z-score: 357.9 E(): 2e-12; 39.7% identity in 234 aa overlap. Contains Pfam match to entry PF01476 PG_binding_2, Putative peptidoglycan binding domain and possible N-terminal region signal peptide sequence 4 4 9 10 [Reporter]SCO4313 (12D21)_PCR/7744 [Gene]SCO4313/7050 NC_003888 SCD95A.46, possible regulatory protein, len: 216 aa; similar to TR:CAB56658 (EMBL:AL121596) Streptomyces coelicolor putative tetR family transcriptional regulator SCF51A.06, 177 aa; fasta scores: opt: 164 z-score: 202.9 E(): 0.00077; 32.0% identity in 103 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 37..58 (+5.35 SD) 4 1 19 9 [Reporter]SCO3709 (14H8)_PCR/1916 [Gene]SCO3709/1751 NC_003888 SCH35.15, possible ECF sigma factor, len: 184 aa; similar to many e.g. SW:RPOE_STRCO sigma E from Streptomyces coelicolor (176 aa) fasta scores; opt: 216, z-score: 250.5, E(): 1.3e-06, (29.2% identity in 161 aa overlap). 4 1 19 8 [Reporter]SCO1654 (15D8)_PCR/1915 [Gene]SCO1654/1750 NC_003888 SCI41.37, possible two-component response regulator, len: 223 aa. Highly similar to various putative two-component response regulator, e.g. TR:CAB40929 (EMBL:AL049630) Streptomyces coelicolor putative SCE126.07C two component response regulator, 224 aa; fasta scores: opt: 866 z-score: 1015.8 E():0; 63.5% identity in 222 aa overlap. Pfam match to entry PF00072 response_reg, response regulator receiver domain also PF00196 GerE, Bacterial regulatory proteins, luxR family. Contains possible helix-turn-helix motif at aa 174-195. (Score 1000, +2.59 SD) 1 3 13 21 [Reporter]SCO2554 (2M10)_PCR/4316 [Gene]SCO2554/3929 NC_003888 SCC77.21c, dnaJ2, DnaJ protein, len: 378 aa. Highly similar to many including: Escherichia coli SW:DNAJ_ECOLI(EMBL:M12565) DnaJ protein (375 aa), fasta scores opt: 1057 z-score: 1055.3 E():0 45.0% identity in 378 aa overlap and Streptomyces albus g TR:O52164(EMBL:AF025656) DnaJ protein (379 aa), fasta scores opt: 2401 z-score: 2385.8 E():0 92.6% identity in 378 aa overlap. Contains a Prosite hit to PS00636 Nt-dnaJ domain signature and Pfam matches to entries PF00226 DnaJ, PF00684 DnaJ_CXXCXGXG and PF01556 DnaJ_C. 1 3 13 20 [Reporter]SCO5903 (3I10)_PCR/4315 [Gene]SCO5903/3928 NC_003888 SC10A5.08, questionable ORF, possible pseudogene fragment, len: 125; contains segment of strong similarity to part of S. coelicolor regulator TR:Q53894 (EMBL:U51332) AbsA2 (222 aa), fasta scores; opt: 110 z-score: 210.7 E(): 0.00 016, 47.1% identity in 70 aa overlap 1 3 13 19 [Reporter]SCO5551 (4E10)_PCR/4314 [Gene]SCO5551/3927 NC_003888 SC1C2.32, unknown, len: 190 aa 1 3 13 18 [Reporter]SCO6407 (5A10)_PCR/4313 [Gene]SCO6407/3926 NC_003888 SC3C8.26, possible gamma-glutamyltranspeptidase (putative secreted protein), len: 603 aa; similar to many e.g. GGT_BACSU gamma-glutamyltranspeptidase precursor (EC 2.3.2.2) (587 aa), fasta scores; opt: 705 z-score: 863.9 E(): 0, 36.9% identity in 591 aa overlap, and to PAC1_PSESV cephalosporin acylase I (EC 3.5.1.-) (557 aa), fasta scores; opt: 377 z-score: 819.8 E(): 0, 34.1% identity in 569 aa overlap. Contains probable N-terminal signal sequence 1 3 13 17 [Reporter]SCO5824 (6M6)_PCR/4312 [Gene]SCO5824/3925 NC_003888 SC5B8.14, probable two-component sensor, len: 384 aa; N-termius has six possible transmembrane domains, C-terminus is similar to histidine kinase domain of many two-component sensors e.g. NARQ_ECOLI P27896 nitrate/nitrite sensor (566 aa) fasta scores; opt: 220 z-score: 227.4 E(): 1.8e-0 5, 32.3% identity in 198 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 13 16 [Reporter]SCO5437 (7I6)_PCR/4311 [Gene]SCO5437/3924 NC_003888 SC6A11.13, possible MerR-family transcriptional regulator, len: 132 aa; similar to many e.g. TR:Q9X5V4 (EMBL:AF127795) heavy metal regulator HmrR from Rhizobium leguminosarum (biovar viciae) (129 aa) fasta scores; opt: 228, z-score: 295.1, E(): 5e-09, 35.7% identity in 129 aa overlap. Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and possible helix-turn-helix motif score 1747 (+5.14 SD) in ID at residue 1 4 4 9 9 [Reporter]SCO3132 (13P17)_PCR/7743 [Gene]SCO3132/7049 NC_003888 SCE66.11c, possible trans-aconitate methyltransferase, len: 303 aa; similar to many e.g. SW:TAM_ECOLI (EMBL:AE000249) trans-aconitate methyltransferase from Escherichia coli (251 aa) fasta scores; opt: 707, z-score: 686.9, E(): 8.8e-31, 43.9% identity in 269 aa overlap. Contains degenerately repetative glycine-rich sequence at the C-terminus. 1 3 13 15 [Reporter]SCO4753 (8E6)_PCR/4310 [Gene]SCO4753/3923 NC_003888 SC6G4.31, unknown, len: 90 aa 4 4 9 8 [Reporter]SCO1908 (14L17)_PCR/7742 [Gene]SCO1908/7048 NC_003888 SCI7.26, possible large secreted protein, len: 613aa; similar to TR:O83555 (EMBL:AE001229) hypothetical protein from Treponema pallidum (622 aa) fasta scores; opt: 478, z-score: 514.7, E(): 2.4e-21, (34.0% identity in 633 aa overlap). Contains possible N-terminal signal sequence. 4 1 19 7 [Reporter]SCO3322 (16P4)_PCR/1914 [Gene]SCO3322/1749 NC_003888 SCE68.20, putative membrane protein, len: 298 aa; unknown function, similar to TR:O33611 (EMBL:AB004855) Streptomyces cyaneus hypothetical protein (277 aa), fasta scores; opt: 463 z-score: 536.3 E(): 1.5e-22, 34.2% identity in 269 aa overlap. C-terminus similar to other hypothetical proteins e.g. SW:YV32_MYCTU (EMBL:Z77162) from Mycobacterium tuberculosis (373 aa) (57.8% identity in 256 aa overlap) and SCH5.21 (EMBL:AL035636) S.coelicolor hypothetical protein (268 aa) (36.3% identity in 267 aa overlap). Contains possible hydrophobic membrane spanning region at C-terminal domain 1 3 13 14 [Reporter]SCO7728 (9A6)_PCR/4309 [Gene]SCO7728/3922 NC_003888 SC8D11.19, conserved hypothetical protein, len: 102 aa; similar to TR:Q9RTC1 (EMBL:AE002024) Deinococcus radiodurans conserved hypothetical protein DR1844, 93 aa; fasta scores: opt: 232 z-score: 318.9 E(): 3.2e-10; 45.8% identity in 83 aa overlap 4 4 9 7 [Reporter]SCO1831 (15H17)_PCR/7741 [Gene]SCO1831/7047 NC_003888 SCI8.16, possible oxidoreductase, len: 251 aa. Similar to several including Streptomyces coelicolor TR:CAB52925 (EMBL: AL109949) putative oxidoreductase SCJ11.40 (250 aa), fasta scores opt: 462 z-score: 489.9 E(): 6.4e-20 33.9% identity in 248 aa overlap and Bacillus megaterium SW:DHG2_BACME (EMBL: D90044) glucose 1-dehydrogenase II (EC 1.1.1.47) (GLCDH-II) (261 aa), fasta scores opt: 412 z-score: 437.9 E(): 5e-17 34.6% identity in 254 aa overlap. Contains Pfam matches to entry PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus. 4 1 19 6 [Reporter]SCO1150 (17L4)_PCR/1913 [Gene]SCO1150/1748 NC_003888 SCG8A.04, rpmE2, 50S ribosomal protein L31, len: 90 aa; strongly similar to many eg. SW:O34967 (YTIA_BACSU) hypothetical ribosomal protein in dps-menE intergenic region from Bacillus subtilis (82 aa) fasta scores; opt: 297, z-score: 406.7, E(): 3.3e-15, 47.6% identity in 82 aa overlap and TR:Q9X8K6 (EMBL:AL049841) 50S ribosomal protein L31 from Streptomyces coelicolor (84 aa) fasta scores; opt: 399, z-score: 539.3, E(): 1.4e-22, 67.5% identity in 83 aa overlap. Contains Pfam match to entry PF01197 Ribosomal_L31, Ribosomal protein L31, Prosite match to PS00030 Eukaryotic putative RNA-binding region RNP-1 signature and Prosite match to PS01143 Ribosomal protein L31 signature. 1 3 13 13 [Reporter]SCO6259 (10M2)_PCR/4308 [Gene]SCO6259/3921 NC_003888 SCAH10.24, probable ABC sugar transport ATP binding protein, len: 263 aa; similar to TR:O68121 (EMBL:AF010496) Rhodobacter capsulatus ribose transport ATP-binding protein, 305 aa; fasta scores: opt: 612 z-score: 706.1 E(): 5.9e-32; 45.6% identity in 217 aa overlap, to SW:RBSA_ECOLI (EMBL:M13169) Escherichia coli, ribose transport ATP-binding protein RbsA, 501 aa; fasta scores: opt: 509 z-score: 585.6 E(): 3e-25; 31.5% identity in 254 aa overlap and to TR:CAA15787 (EMBL:SC7B7) Streptomyces coelicolor St7B7.07, 260 aa; fasta scores: opt: 592 z-score: 521.2 E(): 1e-23; 43.1% identity in 246 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 4 4 9 6 [Reporter]SCO0661 (16D17)_PCR/7740 [Gene]SCO0661/7046 NC_003888 SCF91.21, possible binding protein dependent transport protein, len: 328 aa. Similar to many membrane transport proteins e.g. Escherichia coli SW:UGPA_ECOLI(EMBL:X13141) SN-glycerol-3-phosphate transport system permease protein (295 aa), fasta scores opt: 393 z-score: 465.7 E(): 1.5e-18 28.7% identity in 282 aa overlap and Streptomyces coelicolor TR:CAB41203(EMBL:AL049661) putative binding protein dependent transport protein (327 aa), fasta scores opt: 452 z-score: 533.8 E(): 2.4e-22 28.2% identity in 326 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and multiple possible membrane spanning hydrophobic domains. 4 1 19 5 [Reporter]SCO3335 (18H4)_PCR/1912 [Gene]SCO3335/1747 NC_003888 SCE7.02c, probable AraC-family transcriptional regulator, len: 334 aa; similar to many e.g. TR:CAB40696 (EMBL:AL049587) putative transcriptional regulator (325 aa) fasta scores; opt: 643, z-score: 750.6, E(): 0, (39.9% identity in 316 aa overlap) and TR:O30507 (EMBL:AF008308) from Pseudomonas aeruginosa (328 aa) fasta scores; opt: 360, z-score: 423.1, E(): 3e-16, (27.7% identity in 328 aa overlap). Contains Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. Also contains probable helix-turn-helix motif (+4.06 SD) at 241-262aa. 1 3 13 12 [Reporter]SCO2353 (11I2)_PCR/4307 [Gene]SCO2353/3920 NC_003888 SCC8A.11c, unknown, len: 213aa; weakly similar to others eg. TR:Q9XAQ0 (EMBL:AL078618) putative acetyltransferase from Streptomyces coelicolor (187 aa) fasta scores; opt: 133, z-score: 173.2, E(): 0.035, 27.2% identity in 162 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 4 4 9 5 [Reporter]SCO6834 (17P13)_PCR/7739 [Gene]SCO6834/7045 NC_003888 SC3D9.02c, trxB2, possible thioredoxin reductase (326 aa). Previously sequenced and consequently identical to Streptomyces coelicolor TR:CAB61258 (EMBL:AJ251005). Also highly similar to the other Streptomyces coelicolor trxB CDS: SW:TRXB_STRCO( EMBL:X92105) thioredoxin reductase (EC 1.6.4.5) SCH24.12 (321 aa), fasta scores opt: 1547 z-score: 1709.6 E():0 74.2% identity in 310 aa overlap. Contains a Prosite hit to PS00573 Pyridine nucleotide-disulphide oxidoreductases class-II active site. 4 1 19 4 [Reporter]SCP1.261c (19D4)_PCR/1911 [Gene]SCP1.261c/1746 NC_003888 SCP1.261c, possible secreted protein, len: 167aa; N-terminus strongly similar to that from TR:Q9S1W2 (EMBL:AL109950) hypothetical protein SCJ4.11 from Streptomyces coelicolor. Contains a possible cleavable N-terminal signal sequence. 4 4 9 4 [Reporter]SCO4009 (18L13)_PCR/7738 [Gene]SCO4009/7044 NC_003888 2SC10A7.13c, possible bifunctional protein (histidine kinase and regulator), len: 1463 aa; N-terminal region similar to TR:Q9RRR7 (EMBL:AE002072) Deinococcus radiodurans sensory box sensor histidine kinase DR2419, 744 aa; blastp scores: Score= 273 (96.1 bits), Expect= 3.4e-19, Sum P(2)= 3.4e-19; Identities= 112/386 (29%), Positives= 170/386 (44%) and C-terminal region similar to TR:Q9RKR9 (EMBL:AL133220) Streptomyces coelicolor putative multi-domain regulatory protein SCC75A.05c, 1334 aa; blastp scores: Score= 229 (80.6 bits), Expect= 6.8e-14, Sum P(3)= 6.8e-14; Identitie = 100/311 (32%), Positives= 119/311 (38%). Contains 2x Pfam matches to entry PF00989 PAS, PAS domain, Pfam match to entry PF00512 signal, Histidine kinase and to entry PF00072 response_reg, Response regulator receiver domain. Also contains a 5x degenerated repeat GQ and a 10x degenerated repeat: Q(G/A) and a possible N-terminal region signal peptide sequence 4 1 19 3 [Reporter]SCO1671 (19P24)_PCR/1910 [Gene]SCO1671/1745 NC_003888 SCI52.13, hypothetical protein, len: 772 aa; N-terminal region (first 89 aa) similar to N-terminal region of TR:O05479 (EMBL:M86351) Streptomyces sp. hypothetical protein 481 aa and rest of the CDS similar to C-terminal region of TR:Q9RT63 (EMBL:AE002029) Deinococcus radiodurans exodeoxyribonuclease V, subunit RecD, putative DR1902, 715 aa; fasta scores: opt: 298 Z-score: 242.2 bits: 55.5 E(): 6.5e-06; 31.023% identity in 577 aa overlap 4 4 9 3 [Reporter]SCP1.209c (19H13)_PCR/7737 [Gene]SCP1.209c/7043 NC_003888 SCP1.209c, unknown, len: 331aa; 4 1 19 2 [Reporter]SCO5101 (20H24)_PCR/1909 [Gene]SCO5101/1744 NC_003888 SCBAC28G1.27, conserved hypothetical protein, len: 63 aa; similar to TR:Q9HTF6 (EMBL:AE004953) Pseudomonas aeruginosa hypothetical protein PA5408, 58 aa; fasta scores: opt: 146 Z-score: 225.2 bits: 45.1 E(): 6.2e-05; 45.652% identity in 46 aa overlap 4 4 9 2 [Reporter]SCO0057 (20P9)_PCR/7736 [Gene]SCO0057/7042 NC_003888 SCJ4.38, unknown, len: 46 aa 4 1 18 22 [Reporter]SCO1134 (1H20)_PCR/1907 [Gene]SCO1134/1743 NC_003888 2SCG38.27c, probable oxidoreductase, iron-sulphur binding subunit, len: 200 aa; similar to TR:Q52589 (EMBL:X77931) Pseudomonas thermocarboxydovorans carbon monoxide oxygenase [cytochrome B-561], small chain (EC 1.2.2.4) CutC, 163 aa; fasta scores: opt: 405 z-score: 478.3 E(): 3.4e-19; 45.6% identity in 169 aa overlap and to TR:Q9RK27 (EMBL:AL132856) Streptomyces coelicolor putative oxidoreductase, iron-sulphur binding subunit StF15.10, 211 aa; fasta scores: opt: 729 z-score: 804.0 E(): 0; 62.3% identity in 175 aa overlap. Contains Pfam matches to entries PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains and PF01799 fer2_2, [2Fe-2S] binding domain and matches to Prosite entries PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature and PS00070 Aldehyde dehydrogenases cysteine active site 4 4 8 22 [Reporter]SCO4212 (1P5)_PCR/7734 [Gene]SCO4212/7041 NC_003888 2SCD46.26, unknown, len: 120 aa; similar to TR:Q9ACR7 (EMBL:AL590464) hypothetical protein from Streptomyces coelicolor (122 aa) fasta scores; opt: 369, Z-score: 454.9, E(): 1e-17, 52.000% identity (56.522% ungapped) in 125 aa overlap. 4 1 18 21 [Reporter]SCO6505 (2D20)_PCR/1906 [Gene]SCO6505/1742 NC_003888 SC1E6.14, gvpJ, probable gas vesicle synthesis protein, len: 109 aa; similar to e.g. TR:O68669 (EMBL:AF053765) gas vesicle protein GVPJ from Bacillus megaterium (100 aa), fasta scores; opt: 358 z-score: 597.2 E(): 5.5e-26, 61.6% identity in 86 aa overlap. Contains PS00234 Gas vesicles protein GVPa signature 1 and Pfam match to entry PF00741 Gas_vesicle, Gas vesicle protein, score 60.90, E-value 2.8e-14 4 4 8 21 [Reporter]SCO5378 (2L5)_PCR/7733 [Gene]SCO5378/7040 NC_003888 2SC6G5.22c, chiS, chitinase two-component sensor kinase, len: 398aa; previously sequenced as TR:Q9Z678 (EMBL:AF102271). Similar to many others eg. TR:Q9RHU7 (EMBL:AB016841) sensor kinase from chitinase regulatory system in Streptomyces thermoviolaceus (448 aa) fasta scores; opt: 1663, z-score: 1797.1, E(): 0, 67.4% identity in 396 aa overlap. Contains possible membrane-spanning hydrophobic regions. 4 1 18 20 [Reporter]SCO7697 (3P16)_PCR/1905 [Gene]SCO7697/1741 NC_003888 SC1A4.05, probable secreted hydrolase, len: 435 aa; similar to SW:PHYT_BACSU (EMBL:AF015775) Bacillus subtilis 3-phytase precursor (EC 3.1.3.8) Phy, 382 aa; fasta scores: opt: 203 z-score: 223.6 E(): 6.5e-05; 31.0% identity in 387 aa overlap. Contains Pfam match to entry PF02333 Phytase. Also contains possible N-terminal region signal peptide sequence 4 1 18 19 [Reporter]SCO7225 (4L16)_PCR/1904 [Gene]SCO7225/1740 NC_003888 SC2H12.24, secreted chitinase, len: 244 aa. The N-terminal is highly similar to several chitin binding proteins e.g. Streptomyces reticuli TR:O87962(EMBL:Y14315) chitin binding protein (CHB2) (201 aa), fasta scores opt: 181 z-score: 193.2 E(): 0.0027 38.4% identity in 203 aa overlap. The C-terminal is similar to many chitinases e.g. Streptomyces olivaceoviridis TR:CAB83055(EMBL:AJ276990) chitinase precursor (EC 3.2.1.14) (781 aa), fasta scores opt: 322 z-score: 324.1 E(): 1.4e-10 36.5% identity in 167 aa overlap. Also highly similar to several Streptomyces coelicolor chitinases e.g. TR:Q9Z9M5(EMBL:AB017013) chitinase, ChiG (fragment) (172 aa), fasta scores opt: 845 z-score: 846.9 E():0 67.4% identity in 172 aa overlap and TRO86614:(EMBL:AL031155) SC3A7.13 (252 aa), fasta scores opt: 1060 z-score: 1056.1 E(): 0 75.8% identity in 252 aa overlap. Contains a G/D rich region between residues 171..187. Contains a possible N-terminal signal sequence 1 3 13 11 [Reporter]SCO4457 (12E2)_PCR/4306 [Gene]SCO4457/3919 NC_003888 SCD6.35c, possible integral membrane protein, len: 333 aa. Contains possible hydrophobic membrane spanning regions 1 3 13 10 [Reporter]SCO2858 (13A2)_PCR/4305 [Gene]SCO2858/3918 NC_003888 SCE20.32c, unknown, len: 85 aa. 1 3 13 9 [Reporter]SCO3546 (13M22)_PCR/4304 [Gene]SCO3546/3917 NC_003888 SCH5.09c, possible small lipoprotein, len: 196aa; Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site and probable N-terminal signal sequence. Contains probable coiled-coil from 104 to 141 (38 residues), max score: 1.630, (probability 1.00). 1 3 13 8 [Reporter]SCO0465 (14I22)_PCR/4303 [Gene]SCO0465/3916 NC_003888 SCF76.05c, non-heme chloroperoxidase (chloride peroxidase), len: 276 aa. Identical to Streptomyces lividans SW:PRXC_STRLI (EMBL; U02635) non-heme chloroperoxidase (EC 1.11.1.10) (chloride peroxidase)(CPO-L) (chloroperoxidase L) (275 aa), fasta scores opt: 1866 z-score: 2162.6 E():0 99.3% identity in 275 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 1 3 13 7 [Reporter]SCO0429 (15E22)_PCR/4302 [Gene]SCO0429/3915 NC_003888 SCF51A.07c, possible oxidoreductase, len: 315 aa. Weakly similar to many eukaryotic oxidoreductases e.g. Hordeum vulgare (Barley) TR:Q42837 (EMBL; Z48360) aldose reductase (EC 1.1.1.21) (aldehyde reductase) (320 aa), fasta scores opt: 236 z-score: 263.3 E(): 2.6e-07 27.0% identity in 296 aa overlap and many other putative oxidoreductases e.g. Streptomyces coelicolor TR:CAB42953 (EMBL:AL049863) putative oxidoreductase SC5H1.28C (305 aa), fasta scores opt: 563 z-score: 617.0 E(): 5.2e-27 39.7% identity in 305 aa overlap 1 3 13 6 [Reporter]SCO0462 (16A22)_PCR/4301 [Gene]SCO0462/3914 NC_003888 SCF76.02c, possible oxidoreductase, len: 305 aa. Similar to many hypothetical proteins e.g. Pyrococcus horikoshii SW:APBA_PYRHO (EMBL; AP000006) probable 2-dehydropantoate 2-reductase (EC 1.1.1.169) (ketopantoate reductase) (KPA reductase) (301 aa), fasta scores opt: 359 z-score: 419.9 E(): 4.9e-16 26.5% identity in 291 aa overlap and Streptomyces coelicolor TR:Q9ZBV6 (EMBL:AL034443) putative oxidoreductase SC4B5.12C (323 aa), fasta scores opt: 233 z-score: 274.7 E(): 6.1e-08 32.0% identity in 322 aa overlap. 4 4 8 19 [Reporter]SCO6922 (4D5)_PCR/7731 [Gene]SCO6922/7039 NC_003888 SC1B2.28c, unknown, len: 213 aa. The N-terminus of this protein is highly similar to several proposed regulatory proteins e.g. Streptomyces coelicolor TR:Q9XAL5(EMBL:AL079355) putative transcriptional regulator, SC4C6.25 (81 aa), fasta scores opt: 197 z-score: 226.2 E(): 3.9e-05 41.1% identity in 73 aa overlap. 1 3 13 5 [Reporter]SCO0897 (17M18)_PCR/4300 [Gene]SCO0897/3913 NC_003888 SCM1.30c, unknown, len: 197 aa; similar to the plasmid borne TR:CAC48467 (EMBL:AL603642) Rhizobium meliloti (Sinorhizobium meliloti) hypothetical protein SMB20067, 173 aa; fasta scores: opt: 483 Z-score: 502.3 E(): 2.4e-20; 51.370% identity) in 146 aa overlap 4 4 8 18 [Reporter]SCO6842 (5P1)_PCR/7730 [Gene]SCO6842/7038 NC_003888 SC3D9.10c, unknown, len: 454 aa. 4 1 18 17 [Reporter]SCO7779 (6D16)_PCR/1902 [Gene]SCO7779/1739 NC_003888 SC5E9.27c, possible oxidoreductase, len: 244aa; similar to many eg. SW:P16544 (ACT3_STRCO) putative ketoacyl reductase from Streptomyces coelicolor (261 aa) fasta scores; opt: 236, z-score: 270.7, E(): 1.3e-07, 31.3% identity in 233 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 1 3 13 4 [Reporter]SCP1.141 (18I18)_PCR/4299 [Gene]SCP1.141/3912 NC_003888 SCP1.141, unknown, len: 297aa; 4 4 8 17 [Reporter]SCO5767 (6L1)_PCR/7729 [Gene]SCO5767/7037 NC_003888 SC4H8.06, unknown, probable integral membrane protein, len: 402 aa; weakly similar to several hypotheical proteins from B. subtilis and Methanococcus e.g. TR:E1184257 (EMBL:Z99120) from B. subtilis (369 aa), fasta scores; opt: 375 z-score: 321.7 E(): 1e-10, 24.1% identity in 348 aa overlap. C-terminal region highly similar to TR:Q54391 (EMBL:X76076) Streptomyces lividans RecA gene (fragment) 75 aa; fasta scores: opt: 490 Z-score: 548.1 E(): 6.7e-23; 96.000% identity in 75 aa overlap. Start site chosen by similarity. Contains possible hydrophobic membrane spanning regions 4 1 18 16 [Reporter]SCO5649 (7P12)_PCR/1901 [Gene]SCO5649/1738 NC_003888 SC6A9.18c, unknown, len: 266 aa; similar to M. tuberculosis hypothetical protein TR:O07256 (EMBL:Z96800) MTCY63.37 (261 aa), fasta scores; opt: 508 z-score: 606.3 E(): 1.6e-26, 36.7% identity in 251 aa overlap 1 3 13 3 [Reporter]SCO4906 (19E18)_PCR/4298 [Gene]SCO4906/3911 NC_003888 2SCK8.32c, afsQ2, sensor kinase protein, len: 535 aa; identical to previously sequenced SW:AFQ2_STRCO (EMBL:D10654) Streptomyces coelicolor sensor protein AfsQ2, 535 aa. Contains 3x Pfam matches to entries PF00672 DUF5, HAMP domain, PF00512 signal, Histidine kinase and PF02518 HATPase_c, Histidine kinase. Contains possible hydrophobic membrane spanning region 4 4 8 16 [Reporter]SCO0300 (7H1)_PCR/7728 [Gene]SCO0300/7036 NC_003888 SC5G9.09c, probable monooxygenase, len: 603 aa; similar to several monooxygenases e.g. SW:CYMO_ACISP (EMBL:M19029) Acinetobacter sp cyclohexanone monooxygenase (542 aa), fasta scores; opt: 643 z-score: 715.7 E(): 1.7e-32, 33.6% identity in 518 aa overlap. GC frameplot and codon usage plots suggest the presence of an approx. 50 aa N-terminal extension absent from similar sequences 4 1 18 15 [Reporter]SCO5757 (8L12)_PCR/1900 [Gene]SCO5757/1737 NC_003888 SC7C7.12, questionable ORF, len: 88 aa 1 3 13 2 [Reporter]SCO5087 (20M14)_PCR/4297 [Gene]SCO5087/3910 NC_003888 SCBAC28G1.13, actIORF1, actinorhodin polyketide beta-ketoacyl synthase alpha subunit, len: 467 aa; identical to C-terminal region of SW:KASA_STRCO (EMBL:X63449) Streptomyces coelicolor actinorhodin polyketide putative beta-ketoacyl synthase 1 ActI ORF1, 424 aa. Previously sequenced start codon at residue 44 of this sequence. Also similar to TR:CAB45606 (EMBL:AL079356) Streptomyces coelicolor polyketide beta-ketoacyl synthase alpha (WhiE locus) SC6G9.15, 424 aa; fasta scores: opt: 1706 Z-score: 1660.2 bits: 316.4 E(): 9.5e-87; 61.871% identity in 417 aa overlap. Contains Pfam matches to entries PF00109 ketoacyl-synt, Beta-ketoacyl synthase, N-terminal domain and PF02801 ketoacyl-synt_C, Beta-ketoacyl synthase, C-terminal domain and match to Prosite entry PS00606 Beta-ketoacyl synthases active site 4 4 8 15 [Reporter]SCO2143 (8D1)_PCR/7727 [Gene]SCO2143/7035 NC_003888 SC6G10.16, possible two component system response regulator, len: 221aa; similar to many eg. SW:IRLR_BURPS two component response regulator involved in heavy-metal resistance in Burkholderia pseudomallei (229 aa) fasta scores; opt: 635, z-score: 758.0, E(): 0, (50.0% identity in 220 aa overlap). Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain and Pfam match to entry PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal. 4 1 18 14 [Reporter]SCO2637 (9H12)_PCR/1899 [Gene]SCO2637/1736 NC_003888 SC8E4A.07, possible serine protease (putative secreted protein), len: 1098 aa; similar to TR:O93635 (EMBL:AF015225) Thermococcus stetteri stetterlysin (fragment) PlsT, 891 aa; fasta scores: opt: 564 z-score: 570.6 E(): 2.2e-24; 26.0% identity in 918 aa overlap and to SW:SUBV_BACSU (EMBL:M76590) Bacillus subtilis minor extracellular protease VPR precursor (EC 3.4.21.-), 806 aa; fasta scores: opt: 251 z-score: 254.0 E(): 9.7e-07; 23.8% identity in 820 aa overlap. Contains Pfam match to entry PF00082 Peptidase_S8, Subtilase family PS00136 Serine proteases, subtilase family, aspartic acid active site PS00137 Serine proteases, subtilase family, histidine active site PS00138 Serine proteases, subtilase family, serine active site. Contains also possible N-terminal region peptide signal sequence 4 4 8 14 [Reporter]SCO7191 (8P21)_PCR/7726 [Gene]SCO7191/7034 NC_003888 SC8A11.19c, possible secreted protein, len: 269 aa; similar to TR:Q9S1U9 (EMBL:AL109950) Streptomyces coelicolor hypothetical 25.2 kDa protein SCJ24.24c, 242 aa; fasta scores: opt: 745 z-score: 832.0 E(): 0; 48.8% identity in 244 aa overlap. Contains possible N-terminal region signal peptide sequence 4 1 18 13 [Reporter]SCO6241 (10D12)_PCR/1898 [Gene]SCO6241/1735 NC_003888 SCAH10.06, unknown, len: 110 aa 4 4 8 13 [Reporter]SCO6463 (9L21)_PCR/7725 [Gene]SCO6463/7033 NC_003888 SC9B5.30, hypothetical protein, len: 289aa; similar to many eg. TR:O06216 (EMBL:Z95388) from Mycobacterium tuberculosis (288 aa) fasta scores: opt: 272, z-score: 302.0, E(): 1.6e-09, (30.1% identity in 259 aa overlap). 4 1 18 12 [Reporter]SCO4401 (11P8)_PCR/1897 [Gene]SCO4401/1734 NC_003888 SCD10.33c, possible lipoprotein, len: 438 aa; N-terminal domain similar to N-terminal domain of TR:O68027 (EMBL:AF010496) Rhodobacter capsulatus potential Leu/Ile/Val-binding protein precursor, 400 aa; fasta scores: opt: 151 z-score: 166.0 E(): 0.09; 29.8% identity in 131 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 4 8 12 [Reporter]SCO2425 (10H21)_PCR/7724 [Gene]SCO2425/7032 NC_003888 SCC42.06, possible secreted protein, len: 313 aa. Contains possible N-terminal region signal peptide sequence 4 1 18 11 [Reporter]SCO4637 (12L8)_PCR/1896 [Gene]SCO4637/1733 NC_003888 SCD82.08, hypothetical protein, len: 142 aa; similar to TR:O27971 (EMBL:AE000944) Archaeoglobus fulgidus MaoC protein AF2313, 151 aa; fasta scores: opt: 191 z-score: 246.3 E(): 2.7e-06; 34.0% identity in 106 aa overlap and to TR:Q9CBJ7 (EMBL:AL583923) Mycobacterium leprae hypothetical protein ML1909, 142 aa; fasta scores: opt: 456 Z-score: 548.6 E(): 6.3e-23; 51.471% identity in 136 aa overlap. Contains Pfam match to entry PF01575 MaoC_like, MaoC like domain 4 4 8 11 [Reporter]SCO4075 (11D21)_PCR/7723 [Gene]SCO4075/7031 NC_003888 SCD25.11c, ABC transport protein, ATP-binding subunit, len: 326 aa. Identical to Streptomyces lividans TR:Q04324 (EMBL; X58873) hypothetical 35.0 KD protein in tRNA-glyU beta gene region (326 aa), fasta scores opt: 2092 z-score: 2329.9 E(): 0 100.0% identity in 326 aa overlap and Streptomyces coelicolor proteins TR:CAB50753 (EMBL; AL096839) putative ABC transporter, ATP-binding subunit SCC22.10 (307 aa), fasta scores opt: 648 z-score: 727.6 E(): 0 42.1% identity in 309 aa overlap and TR:CAB42771 (EMBL; AL049841) putative ABC transporter, ATP-binding subunit SCI11.08 (315 aa), fasta scores opt: 583 z-score: 447.5 E(): 1.1e-19 41.3% identity in 320 aa overlap. Contains a Pfam match to entry PF00005 ABC_tran, ABC transporter, PS00211 ABC transporters family signature and a PS00017 ATP/GTP-binding site motif A (P-loop). 4 1 18 10 [Reporter]SCO3237 (13H8)_PCR/1895 [Gene]SCO3237/1732 NC_003888 SCE29.06c, unknown, len: 462 aa; similar to TR:O69954 (EMBL:AL023862) hypothetical protein from Streptomyces coelicolor (465 aa) fasta scores; opt: 2210, z-score: 2646.8, E(): 0, (70.8% identity in 452 aa overlap). 4 4 8 10 [Reporter]SCO2915 (12P17)_PCR/7722 [Gene]SCO2915/7030 NC_003888 SCE19A.15c, hypothetical protein, len: 202 aa; similar to SW:Y03H_MYCLE (EMBL:U00014) Mycobacterium leprae hypothetical protein (222 aa), fasta scores; opt: 192 z-score: 222.4 E(): 4.7e-05, 34.6% identity in 188 aa overlap and SW:Y03H_MYCTU (EMBL:Z73902) Mycobacterium tuberculosis hypothetical protein (218 aa) (32.8% identity in 186 aa overlap) 4 1 18 9 [Reporter]SCO3170 (14D8)_PCR/1894 [Gene]SCO3170/1731 NC_003888 SCE87.21c, probable 3-oxoacyl-[acyl carrier protein] reductase, len: 297 aa. Similar to many including: Mycobacterium tuberculosis TR:O33263 (EMBL:Z97559) probable oxidoreductase (293 aa), fasta scores opt: 672 z-score: 754.5 E(): 0 41.6% identity in 281 aa overlap and Escherichia coli SW:FABG_ECOLI (EMBL:M84991) 3-oxoacyl-[acyl carrier protein] reductase (244 aa), fasta scores opt: 354 z-score: 402.7 E(): 4.6e-15 31.3% identity in 195 aa overlap. Contains a Pfam match to entry PF00106 adh_short, short chain dehydrogenase and a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature. 4 1 18 8 [Reporter]SCO5145 (15P4)_PCR/1893 [Gene]SCO5145/1730 NC_003888 SCP8.08, conserved hypothetical protein, len: 74 aa; highly similar to TR:O05312 (EMBL:Z93777) Mycobacterium tuberculosis hypothetical 7.8 kD protein MTCI364.23, 75 aa; fasta scores: opt: 323 z-score: 419.6 E(): 6.6e-16; 78.6% identity in 70 aa overlap 1 3 12 22 [Reporter]SCO1257 (1M10)_PCR/4295 [Gene]SCO1257/3909 NC_003888 2SCG18.04c, possible ABC transport system integral membrane protein, len: 241 aa; similar to TR:CAB94565 (EMBL:AL359152) Streptomyces coelicolor putative ABC transport system integral membrane protein 2SC6G5.38c, 258 aa; fasta scores: opt: 513 z-score: 578.5 E(): 9.7e-25; 39.8% identity in 256 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 12 21 [Reporter]SCO4856 (2I10)_PCR/4294 [Gene]SCO4856/3908 NC_003888 SC5G8.24c, dhsA, probable succinate dehydrogenase flavoprotein subunit, len: 584aa; strongly similar to many eg. SW:P10444 (DHSA_ECOLI) succinate dehydrogenase flavoprotein subunit from Escherichia coli (588 aa) fasta scores; opt: 1842, z-score: 2076.6, E(): 0, 48.4% identity in 591 aa overlap. Contains Pfam match to entry PF00890 FAD_binding_2, FAD binding domain and Prosite match to PS00504 Fumarate reductase / succinate dehydrogenase FAD-binding site. 1 3 12 20 [Reporter]SCO4813 (3E10)_PCR/4293 [Gene]SCO4813/3907 NC_003888 SCD63A.24, purN, phosphoribosylglycinamide formyltransferase, len: 215 aa; highly similar to TR:AAF05726 (EMBL:AF191543) Mycobacterium paratuberculosis phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) PurN, 209 aa; fasta scores: opt: 708 z-score: 829.2 E(): 0; 57.6% identity in 203 aa overlap. Contains Pfam match to entry PF00551 formyl_transf, Formyl transferase and match to Prosite entry PS00373 Phosphoribosylglycinamide formyltransferase active site 1 3 12 19 [Reporter]SCO5546 (4A10)_PCR/4292 [Gene]SCO5546/3906 NC_003888 SC1C2.27, unknown, len: 261 aa; similar to hypothetical proteins e.g. E. coli TR:P76004 (EMBL:AE000216) O219 (219 aa), fasta scores; opt: 555 z-score: 764.8 E(): 0, 45.7% identity in 208 aa overlap and to e.g. HPCE_ECOLI 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (405 aa), fasta scores; opt: 389 z-score: 507.2 E(): 5.3e-21, 33.9% identity in 218 aa overlap 1 3 12 18 [Reporter]SCO2197 (5M6)_PCR/4291 [Gene]SCO2197/3905 NC_003888 SC3H12.05c, possible integral membrane protein, len: 155 aa; identical to previously sequenced TR:CAB51281 (EMBL:AL096872) Streptomyces coelicolor hypothetical 16.6 kD protein SC5F7.04c, 155 aa and similar to SW:YCKC_BACSU (EMBL:D50453) Bacillus subtilis hypothetical 17.0 kD protein in ComJ 5'region (ORF3) YckC, 151 aa; fasta scores: opt: 108 z-score: 138.4 E(): 2.5; 33.1% identity in 118 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 12 17 [Reporter]SCO5817 (6I6)_PCR/4290 [Gene]SCO5817/3904 NC_003888 SC5B8.07, possible DNA hydrolase with mutT domain, l en: 294 aa; similar in part to many hypothetical proteins e .g Synechocystis sp. TR:P74291 (EMBL:D90913) (261 aa), fasta scores; opt: 241 z-score: 358.0 E(): 9.6e-13, 34.7% ident ity in 251 aa overlap. Contains PS00893 mutT domain signatu re and Pfam match to entry mutT PF00293, Bacterial mutT pro tein, score 39.86 4 4 8 9 [Reporter]SCO3731 (13L17)_PCR/7721 [Gene]SCO3731/7029 NC_003888 SCH22A.09, scoF1, cold-shock protein, len: 67 aa; highly similar to TR:O54117 (EMBL:AL021529) Streptomyces coelicolor cold-shock domain protein SC10A5.26c, 67 aa and to SW:CSPF_STRCO (EMBL:X92686) Streptomyces coelicolor cold shock protein ScoF, 67 aa. Contains Pfam match to entry PF00313 CSD, 'Cold-shock' DNA-binding domain and match to Prosite entry PS00352 'Cold-shock' DNA-binding domain signature 1 3 12 15 [Reporter]SCO4748 (8A6)_PCR/4288 [Gene]SCO4748/3903 NC_003888 SC6G4.26, unknown, len: 78 aa; contains Gln-rich C-t erminus 4 4 8 8 [Reporter]SCO3848 (14H17)_PCR/7720 [Gene]SCO3848/7028 NC_003888 SCH69.18, possible serine/threonine protein kinase, len: 673 aa; similar to many e.g. TR:P71584 (EMBL:Z80233) putative serine/threonine protein kinase from Mycobacterium tuberculosis (626 aa) fasta scores; opt: 1185, z-score: 1028.0, E(): 0, (42.0% identity in 646 aa overlap) and TR:P95308 (EMBL:X99618) serine/threonine protein kinase from Mycobacterium tuberculosis (662 aa) fasta scores; opt: 598, z-score: 522.8, E(): 8.6e-22, (33.9% identity in 345 aa overlap). Also similar to SCGD3.21c from Streptomyces coelicolor (522 aa) fasta scores; opt: 1104, z-score: 1039.5, E(): 0, (50.0% identity in 392 aa overlap). Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain. Also contains possible membrane spanning hydrophobic region. 4 1 18 7 [Reporter]SCO1563 (16L4)_PCR/1892 [Gene]SCO1563/1729 NC_003888 SCL11.19c, possible acetyltransferase, len: 161 aa; similar to TR:O26873 (EMBL:AE000856) Methanobacterium thermoautotrophicum conserved protein Mth779, 170 aa; fasta scores: opt: 174 z-score: 231.8 E(): 1.7e-05; 28.8% identity in 125 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 3 12 14 [Reporter]SCO2652 (9M2)_PCR/4287 [Gene]SCO2652/3902 NC_003888 SC8E4A.22, hypothetical protein, len: 144 aa; similar to TR:P71036 (EMBL:Y08559) Bacillus subtilis hypothetical 14.7 kD protein YwnA, 133 aa; fasta scores: opt: 301 z-score: 389.2 E(): 2.9e-14; 38.4% identity in 138 aa overlap 4 4 8 7 [Reporter]SCO3377 (15D17)_PCR/7719 [Gene]SCO3377/7027 NC_003888 SCE94.28c, unknown, len: 124 aa; similar to hypothetical proteins eg. TR:O32909 (EMBL:AL008609) from Mycobacterium leprae (142 aa) fasta scores; opt: 215, z-score: 273.2, E(): 6.6e-08, (41.9% identity in 105 aa overlap). 4 1 18 6 [Reporter]SCO1081 (17H4)_PCR/1891 [Gene]SCO1081/1728 NC_003888 SCG22.27c, probable electron transfer flavoprotein, alpha subunit, len: 320 aa; similar to SW:ETFA_PARDE (EMBL:L14864) Paracoccus denitrificans electron transfer flavoprotein, alpha subunit EftA, 307 aa; fasta scores: opt: 673 z-score: 707.8 E(): 6e-32; 44.3% identity in 316 aa overlap and to SW:ETFA_MYCTU (EMBL:AL021287) Mycobacterium tuberculosis electron transfer flavoprotein alpha-subunit EftA or MTV012.43c, 318 aa; fasta scores: opt: 1161 z-score: 1212.8 E(): 0; 59.4% identity in 323 aa overlap. Contains Pfam match to entry PF00766 ETF_alpha, Electron transfer flavoprotein alpha subunit and match to Prosite entry PS00696 Electron transfer flavoprotein alpha-subunit signature 1 3 12 13 [Reporter]SCO6255 (10I2)_PCR/4286 [Gene]SCO6255/3901 NC_003888 SCAH10.20, possible dehydrogenase, len: 337 aa; similar to SW:STRI_STRGR (EMBL:Y00459) Stretomyces griseus streptomycin resistance protein StrI, 348 aa; fasta scores: opt: 582 z-score: 670.0 E(): 6.1e-30; 39.5% identity in 344 aa overlap and to SW:MI2D_BACSU (EMBL;M76431) Bacillus subtilis myo-inositol dehydrogenase (EC 1.1.1.18) Idh or IolG OR e83G, 330 aa; fasta scores: opt: 534 z-score: 615.6 E(): 6.5e-27; 28.8% identity in 351 aa overlap. Contains match to Pfam entry PF01408 GFO_IDH_MocA, oxidoreductase family 4 4 8 6 [Reporter]SCO0381 (16P13)_PCR/7718 [Gene]SCO0381/7026 NC_003888 SCF62.07, possible glycosyl transferase, len: 491 aa. Similar to many other glycosyl transferases e.g. Erwinia amylovora SW: AMSG_ERWAM (EMBL; X77921) UDP-galactose-lipid carrier transferase (EC 2.-.-.-) (477 aa), fasta scores opt: 599 z-score: 695.6 E(): 2.2e-31 33.0% identity in 403 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 4 1 18 5 [Reporter]SCO0605 (18D4)_PCR/1890 [Gene]SCO0605/1727 NC_003888 StF55.29, probable araC-family transcriptional regulator, len: 311 aa; similar to TR:CAB46927 (EMBL:AL096822) Streptomyces coelicolor putative arac-family transcriptional regulator SCGD3.05, 334 aa; fasta scores: opt: 893 z-score: 1016.6 E(): 0; 45.7% identity in 315 aa overlap and to SW:RHAS_SALTY (EMBL:X57299) Salmonella typhimurium L-rhamnose operon regulatory protein RhaS, 277 aa; fasta scores: opt: 234 z-score: 272.8 E(): 8e-08; 37.5% identity in 96 aa overlap. Contains match to Pfam entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family and to Prosite entry PS00041 Bacterial regulatory proteins, araC family signature. Contains also a possible helix-turn-helix motif at residues 27077..27142, score 1753 (+5.16 SD) 1 3 12 12 [Reporter]SCO2714 (11E2)_PCR/4285 [Gene]SCO2714/3900 NC_003888 SCC61A.35, possible secreted protein, len: 395aa; contains a possible non-cleavable N-terminal signal sequence. 4 4 8 5 [Reporter]SCO0685 (17L13)_PCR/7717 [Gene]SCO0685/7025 NC_003888 SCF15.06c, unknown, len: 135 aa. 4 1 18 4 [Reporter]SCP1.145 (18P24)_PCR/1889 [Gene]SCP1.145/1726 NC_003888 SCP1.145, possible membrane protein, len: 199aa; contains two possible membrane-spanning hydrophobic regions. 4 4 8 4 [Reporter]SCO0824 (18H13)_PCR/7716 [Gene]SCO0824/7024 NC_003888 SCF43A.14, probable ABC transporter ATP-binding protein, len: 296 aa; similar to many e.g. SW:NOSF_PSEST (EMBL:X53676), nosF, Pseudomonas stutzeri copper transport ATP-binding protein (308 aa), fasta scores; opt: 375 z-score: 445.1 E(): 1.8e-17, 36.8% identity in 223 aa overlap (divergent at the C-terminus). Similar to many from S.coelicolor e.g. SCE7.06c (EMBL:AL049819) probable ABC transporter ATP-binding subunit (276 aa) (40.7% identity in 231 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and PS00017 ATP/GTP-binding site motif A (P-loop) 4 1 18 3 [Reporter]SCO1874 (19L24)_PCR/1888 [Gene]SCO1874/1725 NC_003888 SCI39.21, hypothetical protein, len: 70 aa; similar to C-terminal region of many two-component system response regulators, e.g. TR:Q9K3I0 (EMBL:AL390188) Streptomyces coelicolor putative two-component system response regulator 2SCG18.07, 220 aa; fasta scores: opt: 154 Z-score: 195.9 bits: 41.7 E(): 0.0025; 54.717% identity in 53 aa overlap 4 4 8 3 [Reporter]SCO6283 (19D13)_PCR/7715 [Gene]SCO6283/7023 NC_003888 SC1G7.09, conserved hypothetical protein, len: 281 aa; similar to TR:Q9RDJ2 (EMBL:AL136519) Streptomyces coelicolor hypothetical 29.0 kDa protein SCC57A.04c, 279 aa; fasta scores: opt: 648 Z-score: 723.6 bits: 141.7 E(): 1e-32; 44.912% identity in 285 aa overlap and to TR:O68920 (EMBL:AF058302) Streptomyces roseofulvus hypothetical protein FrnS, 305 aa; fasta scores: opt: 330 Z-score: 372.5 bits: 76.9 E(): 3.7e-13; 34.708% identity in 291 aa overlap 4 1 18 2 [Reporter]SCO7476 (20D24)_PCR/1887 [Gene]SCO7476/1724 NC_003888 SCBAC17A6.09c, possible secreted protein, len: 405aa: no significant database matches. Contains a possible N-terminal signal sequence. 4 4 8 2 [Reporter]SCO0194 (20L9)_PCR/7714 [Gene]SCO0194/7022 NC_003888 SCJ12.06, possible sigma factor, len: 202 aa. Similar to many including: Rhodobacter sphaeroide TR:O33546 (EMBL; U11283) RpoE homologue (168 aa), fasta scores opt: 291 z-score: 373.4 E(): 1.9e-13 35.4% identity in 161 aa overlap and Streptomyces coelicolor TR:O54163 (EMBL; AL021411) putative sigma factor SC7H1.04 (200 aa), fasta scores opt: 255 z-score: 327.4 E(): 7.1e-11 33.7% identity in 184 aa overlap. Contains a PS01063 Sigma-70 factors ECF subfamily signature and a Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily). Contains a possible helix-turn-helix motif between aa's 162..183. 4 1 17 22 [Reporter]SCO1130 (1D20)_PCR/1885 [Gene]SCO1130/1723 NC_003888 2SCG38.23, conserved hypothetical protein, len: 591 aa; similar to TR:P71741 (EMBL:Z81368) Mycobacterium tuberculosis hypothetical 72.7 kDa protein MTCY253.18c, 642 aa; fasta scores: opt: 339 z-score: 399.9 E(): 7.9e-15; 29.0% identity in 628 aa overlap 4 4 7 22 [Reporter]SCO4206 (1L5)_PCR/7712 [Gene]SCO4206/7021 NC_003888 2SCD46.20c, possible AraC-family transcriptional regulator, len: 271 aa; similar to many e.g. TR:CAB88172 (EMBL:AL352972) putative AraC-family transcriptional regulator from Streptomyces coelicolor (278 aa) fasta scores; opt: 434, z-score: 495.7, E(): 4e-20, 36.4% identity in 275 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family. 4 1 17 21 [Reporter]SCO0554 (2P16)_PCR/1884 [Gene]SCO0554/1722 NC_003888 SCF73.01c, manA, probable secreted beta-mannosidase, len: 327 aa; similar to many e.g. MANA_STRLI P51529 mannan endo-1,4-beta-mannosidase precursor (EC 3.2.1.78) (363 aa), fasta scores; opt: 1189 z-score: 1332.7 E(): 0, 61.1% identity in 288 aa overlap. Contains N-terminal signal sequence, and Pfam match to entry PF00150 cellulase, Cellulase (glycosyl hydrolase family 5). Also similar to SC2H4.16 (62.2% identity in 288 aa overlap),SCF11.34c, possible sugar hydrolase, partial CDS, len: >278 aa; C-teminus similar to SW:GUN4_THEFU (EMBL:L20093) Thermomonospora fusca endoglucanase E-4 precursor (EC 3.2.1.4) (endo-1,4-beta-glucanase E-4), CelD, 880 aa; fasta scores: opt: 544 z-score: 555.0 E(): 1.5e-23; 42.8% identity in 215 aa overlap. Contains Pfam matches to entries PF00553 CBD_2, cellulose binding domain and PF00041 fn3, fibronectin type III domain. Overlaps with Streptomyces coelicolor StF73.01c, len: >327 aa; C-terminus similar to many e.g. MANA_STRLI P51529 mannan endo-1,4-beta-mannosidase precursor (EC 3.2.1.78) (363 aa), fasta scores; opt: 1189 z-score: 1332.7 E(): 0, 61.1% identity in 288 aa overlap. Contains N-terminal signal sequence, and Pfam match to entry PF00150 cellulase, Cellulase (glycosyl hydrolase family 5) 4 4 7 21 [Reporter]SCO0787 (2H5)_PCR/7711 [Gene]SCO0787/7020 NC_003888 3SCF60.19, probable secreted hydrolase, len: 422 aa; similar to TR:O68641 (EMBL:AF052745) Oerskovia xanthineolytica beta-1,3-glucanase II BglII, 435 aa; fasta scores: opt: 999 z-score: 1047.9 E(): 0; 52.4% identity in 443 aa overlap. Contains Pfam match to entry PF00722 Glyco_hydro_16, Glycosyl hydrolases family 16 and PF00652 Ricin_B_lectin, Similarity to lectin domain of ricin beta-chain, 3 copies and match to Prosite entry PS01034 Glycosyl hydrolases family 16 active sites. Also contains possible N-terminal region signal peptide sequence 4 1 17 20 [Reporter]SCO7693 (3L16)_PCR/1883 [Gene]SCO7693/1721 NC_003888 SC1A4.01c, probable oxidoreductase, len: 323 aa; similar to SW:OXIR_STRAT (EMBL:M96551) Streptomyces antibioticus probable oxidoreductase (EC 1.-.-.-), 298 aa; fasta scores: opt: 855 z-score: 920.1 E(): 0; 49.8% identity in 305 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase 4 1 17 19 [Reporter]SCO6274 (4H16)_PCR/1882 [Gene]SCO6274/1720 NC_003888 SC2C4.04c, probable type I polyketide synthase, len: 3576 aa; similar to TR:Q9ZGI4 (EMBL:AF079138) Streptomyces venezuelae type I polyketide synthase PikAII, 3739 aa; fasta scores: opt: 3355 z-score: 3346.1 E(): 0; 39.7% identity in 4066 aa overlap. Also highly similar, in this same cosmid, to SC2C4.03c and to SC2C4.05c.. Contains Pfam matches to entries 2x PF00109 ketoacyl-synt, Beta-ketoacyl synthase, 2x PF00698 Acyl_transf, Acyl transferase domain, 2x PF00550 pp-binding, Phosphopantetheine attachment site and matches to Prosite entries 2x PS00606 Beta-ketoacyl synthases active site, 2x PS00216 Sugar transport proteins signature 1, 2x PS00012 Phosphopantetheine attachment site and PS00173 Xylose isomerase signature 2. Also contains a possible coiled-coil region at the N-terminal domain at approx aa 1..25. Protein containing duplicate subunits such that the N-terminal region is equivalent to the C-terminal region 4 4 7 19 [Reporter]SCO6039 (4P1)_PCR/7709 [Gene]SCO6039/7019 NC_003888 SC1C3.27c, probable flavoprotein oxidoreductase, len: 465 aa; similar to many e.g. NAPE_ENTFA NADH peroxidase (EC 1.11.1.1) (447 aa), fasta scores; opt: 642 z-score: 669.2 E(): 4.8e-30, 29.6% identity in 453 aa overlap 4 4 7 18 [Reporter]SCO6837 (5L1)_PCR/7708 [Gene]SCO6837/7018 NC_003888 SC3D9.05, possible arsenic resistance membrane transport protein, len: 368 aa. Highly similar to Streptomyces coelicolor TR:Q9X8Y0(EMBL:AL078610) putative heavy metal resistance membrane protein, SCH35.26 (369 aa), fasta scores opt: 2304 z-score: 2675.9 E(): 0 95.4% identity in 369 aa overlap and Sinorhizobium sp. As4. TR:AAD51846(EMBL:AF178758) membrane subunit of arsenic oxyanion-translocation pump, ArsB (351 aa), fasta scores opt: 1263 z-score: 1469.8 E():0 53.8% identity in 344 aa overlap. Contains possible membrane spanning hydrophobic domains and a Pfam match to entry PF01758 SBF, Sodium Bile acid symporter family. 4 1 17 18 [Reporter]SCO2398 (5D16)_PCR/1881 [Gene]SCO2398/1719 NC_003888 SC4A7.26, possible marR-family transcriptonal regulator, len: 151 aa; similar to TR:Q9WZS3 (EMBL:AE001749) Thermotoga maritima transcriptional regulator, putative, marR family TM0816, 142 aa; fasta scores: opt: 167 z-score: 210.9 E(): 0.00023; 31.7% identity in 139 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 4 4 7 17 [Reporter]SCO7342 (6H1)_PCR/7707 [Gene]SCO7342/7017 NC_003888 SC410.21, unknown, len: 174 aa. Previously sequenced region: Streptomyces coelicolor TR:O52312(EMBL:AF036131) (174 aa). 4 1 17 17 [Reporter]SCO6545 (6P12)_PCR/1880 [Gene]SCO6545/1718 NC_003888 SC5C7.30c, probable secreted cellulase, len: 890 aa; similar to TR:D1029971 (EMBL:AB015511) Aspergillus aculeatus avicelase III (856 aa), fasta scores; opt: 2099 z-score: 1414.5 E(): 0, 44.5% identity in 845 aa overlap, and to many others e.g. GUNA_MICBI endoglucanase A precursor (EC 3.2.1.4) (456 aa), fasta scores; opt: 416 z-score: 340.7 E(): 9.9e-12, 28.5% identity in 449 aa overlap (strongest in the cellulose-binding domain). Contains probable N-terminal signal sequence and Pfam match to entry PF00553 CBD_1, Cellulose binding domain, score 171.70, E-value 8.5e-49 at C-terminus 4 4 7 16 [Reporter]SCO0296 (7D1)_PCR/7706 [Gene]SCO0296/7016 NC_003888 SC5G9.05, possible transcriptional regulator, len: 193 aa; similar to several putative transcriptional regulators e.g. TR:Q54288 (EMBL:X86780) Streptomyces hygroscopicus regulator of antibiotic transport complexes (204 aa), fasta scores; opt: 239 z-score: 291.2 E(): 7.5e-09, 31.9% identity in 182 aa overlap. Similar to TR:O54180 (EMBL:AL021411) S.coelicolor probable transcriptional regulator (203 aa) (35.2% identity in 165 aa overlap). Contains probable helix-turn-helix motif at aa 38-59 (Score 1242, +3.42 SD) 4 1 17 15 [Reporter]SCO5557a (8H12)_PCR/1878 [Gene]SCO5557a/1717 NC_003888 SC7A1.01c, unknown, len: 212 aa; similar to TR:P95112 (EMBL:Z83018) hypothetical protein from Mycobacterium tuberculosis (214 aa) fasta scores; opt: 420, z-score: 422.3, E(): 3.1e-16, (43.5% identity in 207 aa overlap) and TR:O33128 (EMBL:Z99263) hypothetical protein from Mycobacterium leprae (216 aa) fasta scores; opt: 386, z-score: 414.0, E(): 9.1e-16, (40.6% identity in 207 aa overlap). 4 4 7 15 [Reporter]SCO6959 (7P21)_PCR/7705 [Gene]SCO6959/7015 NC_003888 SC6F7.12, possible membrane protein, len: 121 aa. Similar to Bacillus firmus TR:AAF21818(EMBL:AF097740) multiple resistance and pH regulation related protein G (119 aa), fasta scores opt: 256 z-score: 345.4 E(): 8.5e-12 45.5% identity in 99 aa overlap. Contains possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence. 4 1 17 14 [Reporter]SCO2630 (9D12)_PCR/1877 [Gene]SCO2630/1716 NC_003888 SC8E4.05c, possible biotin synthase, len: 246 aa; similar to TR:AAF12013 (EMBL:AE002076) Deinococcus radiodurans biotin synthase putative DR2470, 229 aa; fasta scores: opt: 293 z-score: 315.9 E(): 3.5e-10; 33.0% identity in 209 aa overlap and to SW:BIOY_BACSH (EMBL:M29292) Bacillus sphaericus BioY protein, 196 aa; fasta scores: opt: 275 z-score: 299.1 E(): 3e-09; 35.5% identity in 155 aa overlap. Contains possible hydrophobic membrane spanning regions 4 4 7 14 [Reporter]SCO7187 (8L21)_PCR/7704 [Gene]SCO7187/7014 NC_003888 SC8A11.15c, possible lipoprotein, len: 672 aa; similar to TR:Q98LR9 (EMBL:AP002996) Rhizobium loti probable secreted protein MLL0907, 622 aa; fasta scores: opt: 1110 Z-score: 1188.9 E(): 1.4e-58; 33.122% identity in 631 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 1 17 13 [Reporter]SCO2521 (10P8)_PCR/1876 [Gene]SCO2521/1715 NC_003888 SCC121.24c, unknown, len: 326 aa 4 4 7 13 [Reporter]SCO6054 (9H21)_PCR/7703 [Gene]SCO6054/7013 NC_003888 SC9B1.01c, partial CDS, possible integral membrane protein, len: >149aa; similar to many eg. TR:CAB38499 (EMBL:AL035636) putative integral membrane protein from Streptomyces coelicolor (830 aa) fasta scores; opt: 385, z-score: 428.6, E(): 1.5e-16, (44.7% identity in 141 aa overlap). Contains several possible membrane spanning hydrophobic regions.,SC1B5.14c, probable transmembrane transport protein, partial CDS, len: >381 aa; similar to hypothetical membrane proteins from many organisms e.g. Mycobacterium tuberculosis TR:P96878 (EMBL:Z92771) MTCY71.13 (764 aa), fasta scores; opt: 745 z-score: 768.6 E(): 0, 35.9% identity in 376 aa overlap and to eukaryotic sulfate transpoters TR:O04001 (EMBL:X96761) Sporobolus stapfianus sulphate transporter protein (660 aa), opt: 191 z-score: 224.0 E(): 3e-05, 22.4% identity in 308 aa overlap 4 1 17 12 [Reporter]SCO4397 (11L8)_PCR/1875 [Gene]SCO4397/1714 NC_003888 SCD10.29c, probable ABC transport system ATP-binding protein, len: 263 aa; similar to TR:Q9WZQ0 (EMBL:AE001747) Thermotoga maritima ABC transporter ATP-binding protein TM0793, 260 aa; fasta scores: opt: 591 z-score: 670.1 E(): 7.6e-30; 36.9% identity in 244 aa overlap and to SW:DRRA_STRPE (EMBL:M73758) Streptomyces peucetius daunorubicin resistance ATP-binding protein DrrA, 330 aa; fasta scores: opt: 551 z-score: 623.8 E(): 2.8e-27; 39.1% identity in 225 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 4 7 10 [Reporter]SCO2911 (12L17)_PCR/7700 [Gene]SCO2911/7012 NC_003888 SCE19A.11c, conserved hypothetical protein, len: 92 aa; unknown function, similar to hypothetical proteins from other organisms e.g. SW:Y03K_MYCTU (EMBL:Z73902) Mycobacterium tuberculosis hypothetical protein (93 aa), fasta scores; opt: 341 z-score: 451.3 E(): 8.3e-18, 57.0% identity in 93 aa overlap 4 1 17 11 [Reporter]SCO4472 (12H8)_PCR/1874 [Gene]SCO4472/1713 NC_003888 SCD65.15, possible secreted protein, len: 223 aa; similar to TR:O06392 (EMBL:Z95558) Mycobacterium tuberculosis hypothetical 23.2 kDa protein MTCY25D10.05, 216 aa; fasta scores: opt: 431 z-score: 460.1 E(): 3.7e-18; 39.2% identity in 204 aa overlap. Contains possible N-terminal region signal peptide sequence 4 4 7 9 [Reporter]SCO1073 (13H17)_PCR/7699 [Gene]SCO1073/7011 NC_003888 SCG22.19c, possible hydrogenase, len: 422 aa; similar to SW:BCHP_RHOCA (EMBL:Z11165) Rhodobacter capsulatus geranylgeranyl hydrogenase BchP, 391 aa; fasta scores: opt: 386 z-score: 432.9 E(): 1.2e-16; 33.2% identity in 319 aa overlap. Contains Pfam match to entry PF01494 FAD_binding_3, FAD binding domain 4 1 17 10 [Reporter]SCO3233 (13D8)_PCR/1873 [Gene]SCO3233/1712 NC_003888 SCE29.02, probable hydrolase, len: 272 aa; similar to many e.g. TR:O52809 (EMBL:AJ223998) from the vancomycin biosynthesis cluster of Amycolatopsis orientalis (276 aa) fasta scores; opt: 892, z-score: 1028.9, E(): 0, (50.9% identity in 271 aa overlap) and TR:O67982 (EMBL:AF003947) PcaL; single polypeptide combining 3-oxoadipate enol-lactone hydrolyzing and 4-carboxymuconolactone decarboxylating activityfrom Rhodococcus opacus (400 aa) fasta scores; opt: 288, z-score: 335.4, E(): 2.3e-11, (32.0% identity in 256 aa overlap). Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold, score 77.80, E-value 2.2e-19. 4 4 7 8 [Reporter]SCO0564 (14D17)_PCR/7698 [Gene]SCO0564/7010 NC_003888 SCF73.11c, unknown, len: 386 aa; some similarity to putative secreted protein SC6A5.29 (401 aa), fasta scores; opt: 403 z-score: 496.5 E(): 2.7e-20, 25.9% identity in 347 aa overlap 4 1 17 9 [Reporter]SCO0202 (14P4)_PCR/1872 [Gene]SCO0202/1711 NC_003888 SCJ12.14, unknown, len: 309 aa. Similar to Mycobacterium tuberculosis TR:P95195 (EMBL; Z83867) hypothetical 36.0 KD protein MTCY03A2.27C (332 aa), fasta scores opt: 331 z-score: 377.3 E(): 1.2e-13 28.1% identity in 310 aa overlap and S. coelicolor SCJ12.27c (335 aa), fasta scores opt: 780 z-score: 721.8 E(): 5.9e-35 45.8% identity in 308 aa overlap 4 1 17 8 [Reporter]SCO0665 (15L4)_PCR/1871 [Gene]SCO0665/1710 NC_003888 SCF91.25, possible secreted protein, len: 75 aa. Contains a possible N-terminal signal sequence. 4 4 7 7 [Reporter]SCO0684 (15P13)_PCR/7697 [Gene]SCO0684/7009 NC_003888 SCF15.05c, unknown, len: 258 aa. 4 4 7 6 [Reporter]SCO0140 (16L13)_PCR/7696 [Gene]SCO0140/7008 NC_003888 SCJ33.04, possible merR-family transcriptional regulator, len: 275 aa. Similar to several e.g. Streptomyces coelicolor TR:CAB56383 (EMBL; AL118515) putative merR family transcriptional regulator SCD17.06C (319 aa), fasta scores opt: 164 z-score: 194.6 E(): 0.0018 28.9% identity in 298 aa overlap and Bacillus subtilis SW:BLTR_BACSU (EMBL; L32599) multidrug-efflux transporter 2 regulator (273 aa), fasta scores opt: 154 z-score: 184.2 E(): 0.0069 21.3% identity in 253 aa overlap. Contains a Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family. Contains a possible helix-turn-helix motif located between residues 6..27 (+3.26 SD). 4 1 17 7 [Reporter]SCO3842 (16H4)_PCR/1870 [Gene]SCO3842/1709 NC_003888 SCH69.12, possible dioxygenase, len: 294aa; similar to many catechol dioxygenases eg. TR:O31247 (EMBL:AJ000187) catechol 1.2-dioxygenase from Arthrobacter sp. mA3 (282 aa) fasta scores; opt: 181, z-score: 193.2, E(): 0.002, (32.8% identity in 177 aa overlap). Contains Pfam match to entry PF00775 Dioxygenase, Dioxygenase. Also contains possible membrane spanning hydrophobic region 4 4 7 5 [Reporter]SCO0397 (17H13)_PCR/7695 [Gene]SCO0397/7007 NC_003888 SCF62.23, possible integral membrane protein, len:429 aa. Weakly similar to several proteins of undefined function e.g. Synechocystis sp. (strain PCC 6803) TR:P72591 (EMBL; D90899) iron(III) dicitrate transport system permease protein FecD (349 aa), fasta scores opt: 154 z-score: 170.4 E(): 0.039 25.4% identity in 334 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 1 17 6 [Reporter]SCO0380 (17D4)_PCR/1869 [Gene]SCO0380/1708 NC_003888 SCF62.06, unknown, len: 131 aa. Weakly similar to several Prokaryotic Ank-like proteins e.g. Pseudomonas syringae TR:P95546 (EMBL; AF001355) AnkF precursor (183 aa), fasta scores opt: 255 z-score: 318.0 E(): 2.3e-10 38.4% identity in 125 aa overlap. Contains 2x Pfam match to entry PF00023 ank, Ank repeat. 4 4 7 4 [Reporter]SCO0210 (18D13)_PCR/7694 [Gene]SCO0210/7006 NC_003888 SCJ12.22, hypothetical protein, len: 213 aa. Similar in parts to several hypotheticals e.g. Methanopyrus kandleri SW:IMDH_METKA (EMBL; U31567) OrfX (fragment) (172 aa), fasta scores opt: 251 z-score: 300.4 E(): 2.3e-09 34.5% identity in 116 aa overlap. Also similar to S. coelicolor conserved hypothetical proteins (AL109962) SCJ1.19c (223 aa), fasta scores opt: 411 z-score: 396.5 E(): 7.9e-17 47.3% identity in 201 aa overlap and SCJ1.18 (217 aa), fasta scores opt: 371 z-score: 359.1 E(): 9.5e-15 44.4% identity in 196 aa overlap. Contains 2x Pfam matches to entry PF00571 CBS, CBS domains. 4 1 17 5 [Reporter]SCO0247 (17P24)_PCR/1868 [Gene]SCO0247/1707 NC_003888 SCJ9A.26, unknown, possibly membrane associated, len: 341aa; similar to TR:P71088 (EMBL:Z82044) hypothetical protein from Bacillus subtilis (258 aa) fasta scores; opt: 452, z-score: 475.1, E(): 4.2e-19, (36.6% identity in 262 aa overlap). Also similar to TR:CAB45285 (EMBL:AL079332) hypothetical protein from Streptomyces coelicolor (195 aa) fasta scores; opt: 572, z-score: 600.1, E(): 4.6e-26, (50.0% identity in 184 aa overlap). Contains possible membrane spanning hydrophobic region. 4 4 7 3 [Reporter]SCP1.224c (19P9)_PCR/7693 [Gene]SCP1.224c/7005 NC_003888 SCP1.224c, dnaE, probable DNA polymerase III, alpha chain (EC 2.7.7.7), len: 1213aa; similar to many eg. SW:Q10779 (DP3A_MYCTU) probable DNA polymerase III, alpha chain from Mycobacterium tuberculosis (1184 aa) fasta scores; opt: 3067, z-score: 3382.7, E(): 0, 55.8% identity in 1200 aa overlap and SW:P10443 (DP3A_ECOLI) DNA polymerase III, alpha chain (EC 2.7.7.7) from Escherichia coli (1160 aa) fasta scores; opt: 845, z-score: 927.5, E(): 0, 36.2% identity in 1178 aa overlap. Contains Pfam match to entry PF02231 PHP_N, PHP domain N-terminal region. 4 1 17 4 [Reporter]SCP1.292c (18L24)_PCR/1867 [Gene]SCP1.292c/1706 NC_003888 SCP1.292c, possible regulatory protein, len: 74aa; similar to many eg. TR:Q53896 (EMBL:X60316) AbaA pleiotropic regulatory protein from Streptomyces coelicolor (192 aa) fasta scores; opt: 149, z-score: 226.2, E(): 3.9e-05, 40.4% identity in 57 aa overlap and TR:Q9RHR6 (EMBL:AB025200) ORF76 protein from plasmid pNO33 of Streptomyces albulus (76 aa) fasta scores; opt: 309, z-score: 448.7, E(): 1.6e-17, 60.3% identity in 73 aa overlap. 4 4 7 2 [Reporter]SCP1.86 (20H9)_PCR/7692 [Gene]SCP1.86/7004 NC_003888 SCP1.86, unknown, len: 91aa; 4 1 17 3 [Reporter]SCP1.120 (19H24)_PCR/1866 [Gene]SCP1.120/1705 NC_003888 SCP1.120, unknown, len: 59aa; 4 4 6 22 [Reporter]SCO5384 (1H5)_PCR/7690 [Gene]SCO5384/7003 NC_003888 2SC6G5.28, possible tetR-family transcriptional regulator, len: 213 aa; similar to many proposed transcriptional regulators e.g. TR:CAB88457 (EMBL:AL353815) putative tetR-family transcriptional regulator from Streptomyces coelicolor (215 aa) fasta scores; opt: 370, z-score: 461.9, E(): 3e-18, 36.8% identity in 209 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a helix-turn-helix motif (residue 33-54). 4 1 17 2 [Reporter]SCO0568 (20P20)_PCR/1865 [Gene]SCO0568/1704 NC_003888 St8B3.02c, possible polyprenyl synthetase, len: 279 aa: similar to many e.g. TR:Q54193 (EMBL:L37405) geranylgeranyl pyrophosphate synthase from Streptomyces griseus (425 aa) fasta scores; opt: 479, Z-score: 544.9, 36.242% identity (38.710% ungapped) in 298 aa overlap and SW:P22939 (ISPA_ECOLI) geranyltransferase from Escherichia coli (299 aa) fasta scores; opt: 350, Z-score: 401.9, 31.939% identity (34.855% ungapped) in 263 aa overlap. Contains Prosite match to PS00723 Polyprenyl synthetases signature 1 and Pfam match to entry PF00348 polyprenyl_synt, Polyprenyl synthetase. 4 4 6 21 [Reporter]SCO0781 (2D5)_PCR/7689 [Gene]SCO0781/7002 NC_003888 3SCF60.13, possible anti sigma factor antagonist, len: 166 aa; C-terminal region identical to TR:O85703 (EMBL:AF072709) Streptomyces lividans hypothetical 14.3 kDa protein, 128 aa and similar to SW:RSBV_STRCO (EMBL:AF134889) Streptomyces coelicolor anti-sigma B factor antagonist BldG, 113 aa; fasta scores: opt: 199 z-score: 266.9 E(): 2.4e-07; 31.5% identity in 108 aa overlap. Contains Pfam match to entry PF01740 STAS, STAS domain 4 1 16 22 [Reporter]SCO1327 (1P16)_PCR/1863 [Gene]SCO1327/1703 NC_003888 2SCG61.09, possible integral membrane protein, len: 387 aa; similar to many predicted integral membrane protein especially others from Streptomyces coelicolor e.g. TR:CAB69689 (EMBL:AL137165) putative integral membrane protein (375 aa) fasta scores; opt: 481, z-score: 562.8, E(): 7.2e-24, 35.7% identity in 387 aa overlap. Contains possible membrane-spanning hydrophobic regions 4 4 6 20 [Reporter]SCO2687 (3P1)_PCR/7688 [Gene]SCO2687/7001 NC_003888 SCC61A.08, ribA1, GTP cyclohydrolase II, len: 214 aa; involved in riboflavin biosynthesis, strongly similar to many e.g. SW:P25523 (GCH2_ECOLI) RibA, GTP cyclohydrolase II from Escherichia coli (196 aa) fasta scores; opt: 519, z-score: 634.7, E(): 6.8e-28, 50.3% identity in 175 aa overlap. Also similar to TR:CAA19934 (EMBL:AL031107) from Streptomyces coelicolor (221 aa) fasta scores; opt: 477, z-score: 555.6, E(): 2.1e-25, 50.6% identity in 174 aa overlap. Contains Pfam match to entry PF00925 GTP_cyclohydro2, GTP cyclohydrolase II. 4 1 16 21 [Reporter]SCO2232 (2L16)_PCR/1862 [Gene]SCO2232/1702 NC_003888 SC10B7.27, malR, maltose operon transcriptional repressor, len: 344 aa; identical to previously sequenced SW:MALR_STRCO (EMBL:Y07706) Streptomyces coelicolor maltose operon transcriptional repressor MalR, 344 aa and highly similar to SW:REGL_STRLI (EMBL:X98242) Streptomyces lividans transcriptional regulatory protein RegL, 345 aa; fasta scores: opt: 2098 z-score: 2315.7 E(): 0; 95.4% identity in 345 aa overlap. Contains Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family and match to Prosite entry PS00356 Bacterial regulatory proteins, lacI family signature. Contains a possible helix-turn-helix motif at residues 3..24 (+6.54 SD) 4 4 6 19 [Reporter]SCO6035 (4L1)_PCR/7687 [Gene]SCO6035/7000 NC_003888 SC1C3.23, possible lysine/ornithine decarboxylase, len: 402 aa; simlar to Selenomonas ruminantium TR:D1025847 (EMBL:AB011029) lysine/ornithine decarboxylase (EC 4.1.1.17) Ldc (393 aa), fasta scores; opt: 407 z-score: 521.2 E():8.4e-22, 32.8% identity in 387 aa overlap, and to many eukaryotic ornithine decarboxylases e.g. DCOR_CHICK ornithine decarboxylase (EC 4.1.1.17) (Odc) (450 aa), fasta scores; opt: 407 z-score: 413.4 E(): 8.6e-16, 28.8% identity in 386 aa overlap. Contains PS00878 Orn/DAP/Arg decarboxylases family 2 pyridoxal-P attachment site and PS00879 Orn/DAP/Arg decarboxylases family 2 signature 2 and Pfam match to entry Orn_DAP_Arg_deC PF00278, Pyridoxal-dependent decarboxylases acting on ornithine, lysine, arginine and related substrates, score 188.77 4 1 16 20 [Reporter]SCO7634 (3H16)_PCR/1861 [Gene]SCO7634/1701 NC_003888 SC10F4.07, unknown, len: 633 aa. High content in alanine, arginine, glycine and leucine amino acid residues 4 1 16 19 [Reporter]SCO2106 (4D16)_PCR/1860 [Gene]SCO2106/1700 NC_003888 SC2C1A.02c, possible oxidoreductase, len: 407 aa. Highly similar to several reductases including: Rhodococcus sp. (strain NI86/21) SW:THCD_RHOSN(EMBL:U17130) rhodocoxin reductase (EC 1.18.1.-) (426 aa), fasta scores opt: 792 z-score: 833.9 E():0 36.9% identity in 412 aa overlap and Sphingomonas sp. TR:O65946(EMBL:AJ002606) reductase, RedA2, protein (409 aa), fasta scores opt:794 z-score: 836.2 E():0 40.1% identity in 409 aa overlap 3 4 9 11 [Reporter]SCO4080 (11G21)_PCR/7241 [Gene]SCO4080/6599 NC_003888 SCD25.16c, unknown, len: 194 aa. Shares a low level of similarity with Streptomyces coelicolor TR:CAB44517 (EMBL; AL078618) hypothetical 22.4 KD protein SC10A7.07 (199 aa), fasta scores opt: 155 z-score: 201.1 E(): 0.00076 25.9% identity in 185 aa overlap. Also similar to TR:Q9L1Y1 (EMBL:AL138662) Streptomyces coelicolor hypothetical protein SC8E4A.24, 194 aa; fasta scores: opt: 705 Z-score: 859.5 E(): 3e-40; 57.576% identity in 198 aa overlap 3 4 9 10 [Reporter]SCO4312 (12C21)_PCR/7240 [Gene]SCO4312/6598 NC_003888 SCD95A.45c, hypothetical protein, len: 262 aa; similar to TR:Q9I3L7 (EMBL:AE004578) Pseudomonas aeruginosa hypothetical protein PA1495, 208 aa; fasta scores: opt: 479 Z-score: 529.6 E(): 7.2e-22; 44.330% identity in 194 aa overlap. High content in alanine amino acid residues. Contains also a TTA leucine codon, possible target for bldA regulation 3 4 9 9 [Reporter]SCO2878 (13O17)_PCR/7239 [Gene]SCO2878/6597 NC_003888 SCE6.15, unknown, len: 849 aa. Contains degenerate repeats: 3xNR(N/H)TART and 3xAAGEAAAVA(Q/A). The predicted product of this CDS is also rich in the amino acid Ala and contains coiled-coils between residues 194..257 and 587..612. 3 4 9 8 [Reporter]SCO0957 (14K17)_PCR/7238 [Gene]SCO0957/6596 NC_003888 SCM11.12c, possible oxidoreductase, len: 500 aa; C-terminus similar to SW:OXIR_STRAT (EMBL:M96551) Streptomyces antibioticus probable oxidoreductase (EC 1.-.-.-), 298 aa; fasta scores: opt: 169 z-score: 197.8 E(): 0.0012; 32.0% identity in 150 aa overlap 3 4 9 6 [Reporter]SCO3728 (16C17)_PCR/7236 [Gene]SCO3728/6595 NC_003888 SCH22A.06c, possible membrane protein, len: 243 aa. Contains possible hydrophobic membrane spanning regions 3 4 9 5 [Reporter]SCO1524 (17O13)_PCR/7235 [Gene]SCO1524/6594 NC_003888 SCL2.14c, putative membrane protein, len: 180 aa; similar to TR:O06205 (EMBL:Z95387) Mycobacterium tuberculosis hypothetical 38.1 Kd protein MTCY1A10.24, 351 aa; fasta scores: opt: 179 z-score: 211.7 E(): 0.00021; 43.3% identity in 171 aa overlap. Contains possible hydrophobic membrane spanning region 3 4 9 4 [Reporter]SCP1.161c (18K13)_PCR/7234 [Gene]SCP1.161c/6593 NC_003888 SCP1.161c, possible regulatory protein, len: 268aa; regions similar to many regulatory proteins eg. N-terminus similar to TR:Q9S2B9 (EMBL:AL079353) putative regulatory protein from Streptomyces coelicolor (112 aa) blastp scores; Identities = 50/89 (56%), Positives = 60/89 (67%) and C-terminal region similar to SW:P38133 (RPOE_STRCO) SigE, RNA polymerase sigma-E factor from Streptomyces coelicolor (176 aa) blastp scores; Identities = 36/131 (27%), Positives = 62/131 (47%). Contains TTA encoded leucine at residue 70, possible target for bldA regulation. 3 4 9 3 [Reporter]SCP1.97 (19G13)_PCR/7233 [Gene]SCP1.97/6592 NC_003888 SCP1.97, unknown, len: 138aa; 3 4 9 2 [Reporter]SCO1460 (20O9)_PCR/7232 [Gene]SCO1460/6591 NC_003888 SCL6.17c, unknown, len: 131 aa 3 4 8 22 [Reporter]SCO4210 (1O5)_PCR/7230 [Gene]SCO4210/6590 NC_003888 2SCD46.24c, unknown, len: 164 aa 3 4 8 21 [Reporter]SCO5377 (2K5)_PCR/7229 [Gene]SCO5377/6589 NC_003888 2SC6G5.21c, chiR, chitinase two-component response regulator, len: 213 aa; previously sequenced as TR:Q9Z677 (EMBL:AF102271). Similar to many others eg. TR:Q9RHU6 (EMBL:AB016841) response regulator from chitinase regulatory system in Streptomyces thermoviolaceus (213 aa) fasta scores; opt: 956, z-score: 1085.4, E(): 0, 77.0% identity in 213 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain, Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, Prosite match to PS00622 Bacterial regulatory proteins, luxR family signature and a helix-turn-helix motif (residue 169-190). 3 4 8 19 [Reporter]SCO6921 (4C5)_PCR/7227 [Gene]SCO6921/6588 NC_003888 SC1B2.27c, possible membrane protein, len: 576 aa. Contains a 4xPTTPQSGTDTTQDQG repeat sequence and multiple possible membrane spanning hydrophobic domains. 3 4 8 18 [Reporter]SCO6841 (5O1)_PCR/7226 [Gene]SCO6841/6587 NC_003888 SC3D9.09, unknown, len: 350 aa. Similar to Streptomyces coelicolor TR:Q9X865 (EMBL:AL049661) hypothetical protein SCE134.10 (320 aa), fasta scores opt: 589 z-score: 714.3 E(): 2.2e-32 46.0% identity in 313 aa overlap 3 4 8 17 [Reporter]SCO5766 (6K1)_PCR/7225 [Gene]SCO5766/6586 NC_003888 SC4H8.05, unknown, len: 64 aa; similar to M. tuberculosis hypothetical protein TR:E1173868 (MTV002.03c) (68 aa), fasta scores; opt: 193 z-score: 307.9 E(): 5.8e-10, 40.6% identity in 64 aa overlap. Note that this protein is also upstream of RecA 3 4 8 16 [Reporter]SCO0299 (7G1)_PCR/7224 [Gene]SCO0299/6585 NC_003888 SC5G9.08, possible oxidoreductase, len: 294 aa; shows weak similarity to TR:P06632 (EMBL:M12799) Corynebacterium sp. 2,5-diketo-D-gluconic acid reductase (278 aa), fasta scores; opt: 196 z-score: 229.3 E(): 2.1e-05, 27.6% identity in 286 aa overlap. Similar to many hypothetical oxidoreductases e.g. SW:YDBC_ECOLI (EMBL:X62680), YbcD, Escherichia coli hypothetical oxidoreductase (286 aa) (63.3% identity in 283 aa overlap). Also similar to SC5H1.28C (EMBL:AL049863) Streptomyces coelicolor putative oxidoreductase (305 aa) (46.5% identity in 301 aa overlap) 3 4 8 15 [Reporter]SCO2142 (8C1)_PCR/7223 [Gene]SCO2142/6584 NC_003888 SC6G10.15, possible two component sensor kinase, len: 454 aa; similar to many e.g. SW:CUTS_STRCO CutS, two component sensor kinase involved in the regulation of copper metabolism in Streptomyces lividans 66 (414 aa) fasta scores; opt: 537, z-score: 580.3, E(): 5.1e-25, (34.8% identity in 328 aa overlap). Contains Pfam match to entry PF00512 signal, Signal carboxyl-terminal domain and Pfam match to entry PF00672 DUF5, Domain found in bacterial signal proteins. Also contains a possible membrane spanning hydrophobic region 3 4 8 14 [Reporter]SCO7190 (8O21)_PCR/7222 [Gene]SCO7190/6583 NC_003888 SC8A11.18, hypothetical protein, len: 295 aa; similar to TR:AAF67510 (EMBL:AF170880) Streptomyces sphaeroides NovQ, 271 aa; fasta scores: opt: 177 z-score: 217.8 E(): 0.00012; 24.7% identity in 194 aa overlap 3 4 8 13 [Reporter]SCO6462 (9K21)_PCR/7221 [Gene]SCO6462/6582 NC_003888 SC9B5.29, ogt2, methylated-DNA-protein-cysteine methyltransferase len: 186 aa; similar to many eg. SW:DAT1_BACSU methylated-DNA-protein-cysteine methyltransferase from Bacillus subtilis (165 aa) fasta scores; opt: 437, z-score: 636.5, E(): 3.7e-28, (46.2% identity in 156 aa overlap) and SC1A9.15 (EMBL:AL034446) ogt, methylated-DNA-protein-cysteine methyltransferase from Streptomyces coelicolor (186 aa) fasta scores: opt: 466, z-score: 648.6, E(): 2.6e-31, (52.7% identity in 150 aa overlap). Contains PS00374 Methylated-DNA--protein-cysteine methyltransferase active site and Pfam match to entry PF01035 Methyltrans, 6-O-methylguanine DNA methyltransferase. 3 4 8 12 [Reporter]SCO2424 (10G21)_PCR/7220 [Gene]SCO2424/6581 NC_003888 SCC42.05, possible secreted protein, len: 533 aa. Contains possible N-terminal region signal peptide sequence. High content in alanine amino acid residues 3 4 8 11 [Reporter]SCO4074 (11C21)_PCR/7219 [Gene]SCO4074/6580 NC_003888 SCD25.10c, putative integral membrane protein, len: 271 aa. Identical to Streptomyces lividans TR:Q04320 (EMBL; X58873) hypothetical 28.3 kd protein in tRNA-glyU beta gene region (271 aa), fasta scores opt: 1693 z-score: 1924.6 E():0 100.0% identity in 271 aa overlap and Streptomyces coelicolor TR:CAB50754 (EMBL; AL096839) putative integral membrane transport protein. Contains multiple possible membrane spanning hydrophobic domains. 3 4 8 10 [Reporter]SCO2914 (12O17)_PCR/7218 [Gene]SCO2914/6579 NC_003888 SCE19A.14c, probable amino acid permease, len: 475 aa; similar to many amino acid permeases e.g. SW:ANSP_SALTY (EMBL:U04851), ansP, Salmonella typhimurium L-asparagine permease (497 aa), fasta scores; opt: 1360 z-score: 1551.0 E(): 0, 44.4% identity in 455 aa overlap. Similar to SC5F2A.17 (EMBL:AL049587) probable amino acid permease (489 aa) (44.9% identity in 465 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease and PS00218 Amino acid permeases signature 3 4 8 9 [Reporter]SCO3557 (13K17)_PCR/7217 [Gene]SCO3557/6578 NC_003888 SCH5.20c, possible septum site associated protein, len: 396aa; similar to many eg. TR:O33612 (EMBL:AB004855) involved in inhibition of morphological differentiation in Streptomyces azureus (260 aa) fasta scores; opt: 1510, z-score: 1580.5, E(): 0, (89.1% identity in 256 aa overlap) and SW:MIND_BACSU MinD, septum site determining protein from Bacillus subtilis (268 aa) fasta scores; opt: 177, z-score: 193.3, E(): 0.0019, (22.7% identity in 242 aa overlap). 3 4 8 8 [Reporter]SCO0550 (14G17)_PCR/7216 [Gene]SCO0550/6577 NC_003888 SCF11.30, possible membrane protein, len: 292 aa; similar to TR:Q9ZBQ4 (EMBL:AL034447) Streptomyces coelicolor putative transmembrane protein, 396 aa; fasta scores: opt: 677 z-score: 505.6 E(): 8.6e-21; 43.7% identity in 309 aa overlap. Contains possible hydrophobic membrane spanning region 3 4 8 7 [Reporter]SCO1813 (15C17)_PCR/7215 [Gene]SCO1813/6576 NC_003888 SCI28.07, possible gntR-family transcriptional regulator, len: 230aa; similar to many eg. TR:O33960 (EMBL:U78309) pyruvate dehydrogenase complex repressor from Rhodobacter capsulatus (257 aa) fasta scores; opt: 326, z-score: 383.3, E(): 5.2e-14, (34.2% identity in 228 aa overlap). Also similar to TR:O68331 (EMBL:AF033674) hypothetical protein from Pseudomonas marginalis pv. alfalfae (217 aa) fasta scores; opt: 407, z-score: 476.9, E(): 3.1e-19, (40.4% identity in 166 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. 3 4 8 6 [Reporter]SCO2896 (16O13)_PCR/7214 [Gene]SCO2896/6575 NC_003888 SCE6.33c, possible integral membrane protein, len: 268 aa. Similar to a protein of undefined function from Deinococcus radiodurans TR:AAF09788(EMBL:AE001882) conserved hypothetical protein (275 aa), fasta scores: opt: 521 z-score: 606.4 E(): 2.4e-26 38.6% identity in 272 aa overlap. Also weakly similar to the adjoining CDS SCE6.32c, fasta scores: opt: 174 z-score: 186.6 E(): 6.9e-05 29.4% identity in 269 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 3 4 8 5 [Reporter]SCO0245 (17K13)_PCR/7213 [Gene]SCO0245/6574 NC_003888 SCJ9A.24c, possible integral membrane protein, len: 339aa; similar to many hypothetical proteins eg. SW:Y0D3_MYCTU from Mycobacterium tuberculosis (345 aa) fasta scores; opt: 598, z-score: 676.4, E(): 2.6e-30, (34.2% identity in 333 aa overlap). Contains two Pfam matches to entry PF00571 CBS, CBS domain and a Pfam match to entry PF01595 DUF21. Also contains possible membrane spanning hydrophobic regions in the N-terminal region. 3 4 8 4 [Reporter]SCO0718 (18G13)_PCR/7212 [Gene]SCO0718/6573 NC_003888 SCF42.28, unknown, len: 73 aa. 3 4 8 3 [Reporter]SCO6284 (19C13)_PCR/7211 [Gene]SCO6284/6572 NC_003888 SC1G7.10, probable decarboxylase, len: 530 aa; similar to TR:AAK06793 (EMBL:AF324838) Streptomyces antibioticus putative simocyclinone biosynthesis decarboxylase involved in polyketide formation SimA12, 528 aa; fasta scores: opt: 2751 Z-score: 3055.0 bits: 574.9 E(): 1.4e-162; 75.803% identity in 529 aa overlap and to TR:O86517 (EMBL:AL031124) Streptomyces coelicolor putative carboyl transferase SC1C2.16, 527 aa; fasta scores: opt: 2715 Z-score: 3015.0 bits: 567.6 E(): 2.4e-160; 75.094% identity in 530 aa overlap. Contains Pfam match to entry PF01039 Carboxyl_trans, Carboxyl transferase domain 2 3 13 22 [Reporter]SCO1287 (1B14)_PCR/4821 [Gene]SCO1287/4399 NC_003888 2SCG18.34c, hypothetical protein, len: 320 aa; similar to C-terminal region of TR:O31566 (EMBL:Z99108) Bacillus subtilis YfiX protein, 606 aa; fasta scores: opt: 252 z-score: 295.6 E(): 5.6e-09; 27.5% identity in 306 aa overlap 3 4 7 22 [Reporter]SCO4205 (1K5)_PCR/7208 [Gene]SCO4205/6571 NC_003888 2SCD46.19, conserved hypothetical protein, len: 168aa; strongly similar to others from mycobacteria eg. SW:Q11153 (Y487_MYCTU) hypothetical protein from Mycobacterium tuberculosis (183 aa) fasta scores; opt: 508, z-score: 635.1, E(): 6.9e-28, 48.4% identity in 157 aa overlap 2 3 13 21 [Reporter]SCO3878 (2N10)_PCR/4820 [Gene]SCO3878/4398 NC_003888 SCH18.15c, dnaN, DNA polymerase III, beta chain, len: 376 aa; identical to previously sequenced SW:DP3B_STRCO (EMBL:M82836) Streptomyces coelicolor DNA polymerase III, beta chain (EC 2.7.7.7) DnaN, 376 aa. Contains Pfam match to entry PF00712 DNA_pol3_beta, DNA polymerase III beta subunit 3 4 7 21 [Reporter]SCO0786 (2G5)_PCR/7207 [Gene]SCO0786/6570 NC_003888 3SCF60.18, possible integral membrane protein, len: 179 aa. Contains possible hydrophobic membrane spanning regions 2 3 13 20 [Reporter]SCO5904 (3J10)_PCR/4819 [Gene]SCO5904/4397 NC_003888 SC10A5.09c, unknown, len: 126 aa; similar to Mycobac terium tuberculosis hypothetical protein TR:O06156 (EMBL:Z9 5555) MTCY06F7.02C (105 aa), fasta scores; opt: 125 z-score : 213.5 E(): 0.00011, 37.7% identity in 69 aa overlap 2 3 13 19 [Reporter]SCO5552 (4F10)_PCR/4818 [Gene]SCO5552/4396 NC_003888 SC1C2.33c, probable regulator, len: 238 aa; similar to many e.g. GYLR_STRGR glycerol operon regulatory protein (254 aa), fasta scores; opt: 146 z-score: 237.0 E(): 5.9e-06, 28.9% identity in 246 aa overlap 2 3 13 18 [Reporter]SCO6410 (5B10)_PCR/4817 [Gene]SCO6410/4395 NC_003888 SC3C8.29, possible DNA-binding protein, len: 83 aa; contains probable helix-turn-helix motif at aa 29 to 50 (Score 1589, +4.60 SD)) 2 3 13 17 [Reporter]SCO5825 (6N6)_PCR/4816 [Gene]SCO5825/4394 NC_003888 SC5B8.15, probable two-component transcriptional regulator, len: 229 aa; similar to many e.g. DEGU_BACSU P13800 transcriptional regulator (229 aa), fasta scores; opt: 485 z-score: 582.9 E(): 2.8e-25, 37.1% identity in 221 aa overlap. Contains PS00622 Bacterial regulatory proteins, luxR f amily signature, Pfam match to entry response_reg PF00072, Response regulator receiver domain, score 142.77, Pfam match to entry GerE PF00196, Bacterial regulatory proteins, luxR family, score 80.81, and probable helix-turn-helix from aa 180-201 (Score 1092, +2.91 SD) 2 3 13 16 [Reporter]SCO5438 (7J6)_PCR/4815 [Gene]SCO5438/4393 NC_003888 SC6A11.14, trxA2, thioredoxin, len: 104 aa; similar to many e.g. SW:P52230 (THIO_STRCO) thioredoxin TrxA from Streptomyces coelicolor (110 aa) fasta scores: opt: 360, z-score: 470.8, E(): 8.2e-19, 47.5% identity in 101 aa overlap. Contains Pfam match to entry PF00085 thiored, Thioredoxin and Prosite match to PS00194 Thioredoxin family active site 2 3 13 15 [Reporter]SCO4754 (8F6)_PCR/4814 [Gene]SCO4754/4392 NC_003888 SC6G4.32, probable transcriptional regulator, len: 355 aa; similar to many members of the lacI family e.g. RBSR _ECOLI ribose operon repressor (329 aa), fasta scores; opt: 588 z-score: 718.4 E(): 9.4e-33, 34.8% identity in 305 aa overlap. Contains PS00356 Bacterial regulatory proteins, lacI family signature, helix-turn-helix motif at aa 20-41 (Score 2255, +6.87 SD) and Pfam matches to entry PF00356 lacI, Bacterial regulatory proteins, lacI family, score 36.90, E -value 6.1e-09 and to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family, score 127.80, E-va lue 2e-34 2 3 13 14 [Reporter]SCO7729 (9B6)_PCR/4813 [Gene]SCO7729/4391 NC_003888 SC8D11.20, hypothetical protein, len: 332 aa; highly similar to TR:Q9RJG6 (EMBL:AL121600) Streptomyces coelicolor hypothetical 34.3 kDa protein SCF76.08c, 325 aa; fasta scores: opt: 1541 z-score: 1742.5 E(): 0; 71.4% identity in 311 aa overlap 2 1 3 2 [Reporter]SCO6293 (20F8)_PCR/549 [Gene]SCO6293/499 NC_003888 SCBAC8D1.06, conserved hypothetical protein, len: 333 aa; similar to TR:Q9I489 (EMBL:AE004555) Pseudomonas aeruginosa hypothetical protein PA1255, 344 aa; fasta scores: opt: 872 Z-score: 981.3 bits: 190.0 E(): 4.5e-47; 41.964% identity in 336 aa overlap and to TR:Q98F20 (EMBL:AP003003) Rhizobium loti proline racemase MLL3979, 333 aa; fasta scores: opt: 985 Z-score: 1108.1 E(): 4.4e-54; 46.951% identity in 328 aa overlap 2 3 13 13 [Reporter]SCO6260 (10N2)_PCR/4812 [Gene]SCO6260/4390 NC_003888 SCAH10.25, possible sugar kinase, len: 382 aa; similar to SW:GLK_STRCO (EMBL:X65932) Streptomyces coelicolor glucokinase (EC 2.7.1.2) (glucose kinase) Glk, 317 aa; fasta scores: opt: 1022 z-score: 1142.9 E(): 0; 46.6% identity in 311 aa overlap and to TR:CAB51974 EMBL:SC6E10 Streptomyces coelicolor SC6E10.20c, 317 aa; fasta scores: opt: 1022 z-score: 1035.4 E(): 0; 46.6% identity in 311 aa overlap. Contains a match to Pfam entry PF00480 ROK, ROK family and Prosite entry PS01125 ROK family signature 2 1 2 22 [Reporter]SCO5361 (1F4)_PCR/547 [Gene]SCO5361/498 NC_003888 2SC6G5.05, probable methylase, len: 281aa; similar to many eg. TR:P72542 (EMBL:U60417) PapM from Streptomyces pristinaespiralis which catalyses the two successive N-methylation steps of 4-amino-L-phenylalanine leading to DMPAPA via 4-methylamino-L-phenylalanine (292 aa) fasta scores; opt: 723, z-score: 865.6, E(): 0, 44.5% identity in 265 aa overlap. Contains Prosite match to PS00092 N-6 Adenine-specific DNA methylases signature and a possible membrane-spanning hydrophobic region. 2 1 2 21 [Reporter]SCO0771 (2B4)_PCR/546 [Gene]SCO0771/497 NC_003888 3SCF60.03c, conserved hypothetical protein, len: 145 aa; identical to TR:O85694 (EMBL:AF072709) Streptomyces lividans hypothetical 17.2 kDa protein, 145 aa 2 1 2 20 [Reporter]SCO4570 (2N24)_PCR/545 [Gene]SCO4570/496 NC_003888 SCD16A.13c, nuoI, NADH dehydrogenase subunit, len: 211 aa; similar to many e.g. TR:P95173 (EMBL:Z83867) NuoI, NADH dehydrogenase subunit from Mycobacterium tuberculosis (211 aa) fasta scores; opt: 825, z-score: 979.0, E(): 0, (70.1% identity in 164 aa overlap) and SW:NUOI_ECOLI, NADH dehydrogenase subunit NuoI from Escherichia coli (180 aa) fasta scores; opt: 394, z-score: 474.3, E(): 4.3e-19, (39.3% identity in 163 aa overlap). Contains Pfam match to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains and 2 Prosite matches to PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. 2 1 2 19 [Reporter]SCO6147 (3J24)_PCR/544 [Gene]SCO6147/495 NC_003888 SC1A9.11c, oxidoreductase, len: 418 aa, similar to many eg. TR:P97011 (EMBL:AB000519) proposed sorbitol oxidase from Streptomyces sp. (420 aa) fasta scores; opt: 1544, z-score: 1630.2, E():0, (60.0% identity in 413 aa overlap) and TR:O50531 (EMBL:AL009204) FAD-dependent oxidoreductase from Streptomyces coelicolor (445 aa) fasta scores; opt: 374, z-score: 499.4, E(): 1.6e-20, (31.4% identity in 439 aa overlap). 2 1 2 18 [Reporter]SCO6204 (4F24)_PCR/543 [Gene]SCO6204/494 NC_003888 SC2G5.25c, probable catalase, len: 487 aa; similar to many, most similar to SW:BCA_STRVL bromoperoxidase-catalase from Streptomyces violaceus (483 aa) fasta scores; opt: 2334,z-score: 2595.9, E(): 0, (69.4% identity in 487 aaoverlap).Contains PS00437 and PS00438 Catalase proximal heme-ligand signatures. Also contains Pfam match to entry PF00199 catalase, Catalase, score 1008.70, E-value 1.3e-299. 2 1 2 17 [Reporter]SCO6557 (5B24)_PCR/542 [Gene]SCO6557/493 NC_003888 SC4B5.07c, possible neuraminidase (putative secreted protein), len: 648 aa; similar to several e.g. TR:D1001316 (EMBL:D01045) neuraminidase from Micromonospora viridifaciens (647 aa) fasta scores; opt: 286, z-score: 289.0, E(): 8.4e-09, (30.4% identity in 598 aa overlap). Contains possible N-terminal region signal peptide sequence 2 1 2 16 [Reporter]SCO7308 (6N20)_PCR/541 [Gene]SCO7308/492 NC_003888 SC5F8.18, possible membrane transport protein, len: 204 aa. Highly similar to many including: Corynebacterium glutamicum SW:LYSE_CORGL(EMBL:X96471) lysine exporter protein, LysE (236 aa), fasta scores opt: 201 z-score: 247.8 E(): 2.5e-06 34.8% identity in 224 aa overlap and to many hypothetical proteins e.g. Mycobacterium tuberculosis SW:YW33_MYCTU(EMBL:Z74025) (199 aa), fasta scores opt: 668 z-score: 801.1 E(): 0 54.0% identity in 200 aa overlap. Contains a Pfam match to entry PF01810 LysE, LysE type translocator and multiple possible membrane spanning hydrophobic domains. 2 1 2 15 [Reporter]SCO4419 (7J20)_PCR/540 [Gene]SCO4419/491 NC_003888 SC6F11.17c, conserved hypothetical protein, len: 141 aa; similar to TR:O52559 (EMBL:AF040570) Amycolatopsis mediterranei hypothetical 18.7 kDa protein, 166 aa; fasta scores: opt: 372 z-score: 489.5 E(): 9.3e-20; 44.3% identity in 140 aa overlap 2 1 2 14 [Reporter]SCO6882 (8F20)_PCR/539 [Gene]SCO6882/490 NC_003888 SC7F9.34c, possible membrane protein, len: 230 aa. Highly similar to Streptomyces coelicolor TR:Q9X869(EMBL:AL049661) putative secreted protein, SCE134.14 (226 aa), fasta scores opt: 694 z-score: 716.2 E(): 1.8e-32 51.1% identity in 223 aa overlap. Contains a possible membrane spanning hydrophobic domain in the N-terminus. 3 4 7 19 [Reporter]SCO6038 (4O1)_PCR/7205 [Gene]SCO6038/6569 NC_003888 SC1C3.26c, probable integral membrane protein, len:383 aa; start uncertain, similar to hypothetical proteins e.g. Aquifex aeolicus TR:G2983747 (EMBL:AE000734) AQ_1327 (227 aa), fasta scores; opt: 472 z-score: 416.5 E(): 5.7e-16, 37.5% identity in 261 aa overlap. Contains PS00141 Eukaryotic and viral aspartyl proteases active site 3 4 7 18 [Reporter]SCO6833 (5K1)_PCR/7204 [Gene]SCO6833/6568 NC_003888 SC3D9.01, probable isobutyryl-CoA mutase, small subunit (EC 5.4.99.13), len: 159 aa. Highly similar to Streptomyces cinnamonensis TR:CAB59633 (EMBL:AJ246005) isobutyryl-CoA mutase, small subunit (EC 5.4.99.13) (136 aa), fasta scores opt: 580 z-score: 685.3 E(): 9.2e-31 68.3% identity in 126 aa overlap and to the C-terminus of Streptomyces cinnamonensis SW:MUTB_STRCM (EMBL:L10064) methylmalonyl-CoA mutase alpha-subunit (EC 5.4.99.2) (733 aa), fasta scores opt: 291 z-score: 341.2 E(): 1.4e-11 40.5% identity in 111 aa overlap. 3 4 7 17 [Reporter]SCO7340 (6G1)_PCR/7203 [Gene]SCO7340/6567 NC_003888 SC410.19, unknown, len: 121 aa. Similar to Streptomyces coelicolor TR:Q9S1U3(EMBL:AL109950) hypothetical 11.6 kd protein, SCJ4.30 (107 aa), fasta scores opt: 285 z-score: 344.7 E(): 9.8e-12 54.3% identity in 92 aa overlap. 3 4 7 16 [Reporter]SCO0295 (7C1)_PCR/7202 [Gene]SCO0295/6566 NC_003888 SC5G9.04c, probable transmembrane efflux protein, len: 489 aa; similar to many e.g. TR:Q54806 (EMBL:X84072), ptr, Streptomyces pristinaespiralis multidrug resistance integral membrane protein (501 aa), fasta scores; opt: 975 z-score: 1038.5 E(): 0, 37.1% identity in 421 aa overlap. Similar to others from S.coelicolor e.g. TR:Q9ZBW5 (EMBL:AL034443) S.coelicolor probable integral membrane efflux protein (504 aa) (38.8% identity in 484 aa overlap). Contains hydrophobic, possible membrane-spanning regions and PS00216 Sugar transport proteins signature 1 3 4 7 15 [Reporter]SCO6958 (7O21)_PCR/7201 [Gene]SCO6958/6565 NC_003888 SC6F7.11, possible membrane protein, len: 105 aa. Similar to two potassium-efflux system proteins from Rhizobium meliloti: TR:Q52983(EMBL:X93358) K-efflux system protein, PhaF (92 aa), fasta scores opt: 160 z-score: 228.2 E(): 2.8e-05 34.9% identity in 86 aa overlap and SW:PHF2_RHIME(EMBL:L37353) PhaF2 (123 aa), fasta scores opt: 166 z-score: 234.3 E(): 1.3e-05 37.5% identity in 96 aa overlap. Contains N-terminal membrane spanning hydrophobic domains. 3 4 7 14 [Reporter]SCO7186 (8K21)_PCR/7200 [Gene]SCO7186/6564 NC_003888 SC8A11.14, possible integral membrane protein, len: 101 aa. Contains possible hydrophobic membrane spanning regions 3 4 7 13 [Reporter]SCO7173 (9G21)_PCR/7199 [Gene]SCO7173/6563 NC_003888 SC8A11.01, possible transcriptional regulator (fragment), len: >750 aa; similar to TR:Q9S1Z4 (EMBL:AL109747) Streptomyces coelicolor probable transcriptional regulator SCJ21.13, 919 aa; fasta scores: opt: 818 z-score: 913.7 E(): 0; 32.6% identity in 775 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Also contains a possible helix-turn-helix motif at residues 699..720 (+3.25 SD),SC9A4.35, possible regulatory protein (fragment), len: >191 aa; similar to N-terminal region of TR:Q9S1Z4 (EMBL:AL109747) Streptomyces coelicolor probable transcriptional regulator SCJ21.13, 919 aa; fasta scores: opt: 470 z-score: 538.6 E(): 1.6e-22; 44.4% identity in 187 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 4 7 12 [Reporter]SCO2420 (10C21)_PCR/7198 [Gene]SCO2420/6562 NC_003888 SCC42.01c, possible ABC-transporter integral membrane protein (fragment), len: >161 aa; similar to TR:O53545 (EMBL:AL022022) Mycobacterium tuberculosis hypothetical 29.6 kD protein MTV023.07c, 280 aa; fasta scores: opt: 565 z-score: 630.0 E(): 1.3e-27; 54.5% identity in 154 aa overlap. Contains possible hydrophobic membrane spanning regions,SC8A2.08c, conserved hypothetical protein (fragment), len: >139 aa; similar to TR:O53966 (EMBL:AL022073) Mycobacterium tuberculosis hypothetical 28.1 kDa protein MTV051.03, 271 aa; fasta scores: opt: 474 z-score: 595.0 E(): 1.3e-25; 48.6% identity in 138 aa overlap 2 3 13 12 [Reporter]SCO2354 (11J2)_PCR/4811 [Gene]SCO2354/4389 NC_003888 SCC8A.12, unknown, len: 111aa; 3 4 7 11 [Reporter]SCO4527 (11O17)_PCR/7197 [Gene]SCO4527/6561 NC_003888 SCD35.34, possible integral membrane protein, len: 345 aa. Contains possible hydrophobic membrane spanning regions 2 3 13 11 [Reporter]SCO4769 (12F2)_PCR/4810 [Gene]SCO4769/4388 NC_003888 SCD63.01, ECF sigma factor, len: 195 aa; identical to previously sequenced TR:O86843 (EMBL:AJ010601) Streptomyces coelicolor ECF sigma factor, 195 aa 3 4 7 10 [Reporter]SCO2910 (12K17)_PCR/7196 [Gene]SCO2910/6560 NC_003888 SCE19A.10c, probable cysteine synthase, len: 316 aa; similar to many e.g. SW:CYSM_ECOLI (EMBL:M32101), CysM, Escherichia coli cysteine synthase B (303 aa), fasta scores; opt: 776 z-score: 884.3 E(): 0, 43.7% identity in 293 aa overlap. Highly similar to SW:CYSK_MYCTU (EMBL:Z73902) Mycobacterium tuberculosis probable cysteine synthase (323 aa) (72.4% identity in 322 aa overlap). Weak similarity to the N-terminus of SCF43A.11c (EMBL:AL096837) S.coelicolor probable threonine dehydratase (325 aa) (27.2% identity in 195 aa overlap). Contains Pfam match to entry PF00291 S_T_dehydratase, Pyridoxal-phosphate dependent enzymes and PS00901 Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site 2 3 13 10 [Reporter]SCO2859 (13B2)_PCR/4809 [Gene]SCO2859/4387 NC_003888 SCE20.33c, unknown, len: 125 aa. Similar several other proteins of unknown function e.g. from Lactococcus lactis TR:O87247(EMBL:AE001272) conserved hypothetical protein (114 aa), fasta scores opt: 226 z-score: 311.1 E(): 6e-10 35.1% identity in 94 aa overlap. 2 3 13 9 [Reporter]SCO0447 (13N22)_PCR/4808 [Gene]SCO0447/4386 NC_003888 SCF51A.25, possible MarR-family regulatory protein, len: 148 aa. Similar to many hypothetical proteins with similarity to the MarR family of transcriptional regulators e.g. Mycobacterium tuberculosis SW: YZ08_MYCTU (EMBL; Z73101) hypothetical 15.6 KD protein CY31.08 (143 aa), fasta scores opt: 256 z-score: 328.8 E(): 5.9e-11 34.1% identity in 135 aa overlap. Contains a Pfam match to entry PF01047 MarR, MarR family 2 3 13 8 [Reporter]SCO4616 (14J22)_PCR/4807 [Gene]SCO4616/4385 NC_003888 SCD39.16c, xis, excisionase, len: 61 aa; identical to previously sequenced TR:Q53965 (EMBL:X71358) Streptomyces coelicolor Xis, plasmid SLP1 DNA for xis and int genes, 61 aa 2 3 13 7 [Reporter]SCO3933 (15F22)_PCR/4806 [Gene]SCO3933/4384 NC_003888 SCQ11.16, possible regulatory protein, len: 116 aa; similar to TR:Q53733 (EMBL:Z34987), pra, Streptomyces ambofaciens plasmid pSAM2 positive regulator of replicase, excisionase, and integrase genes (116 aa), fasta scores; opt: 161 z-score: 220.6 E(): 5.9e-05, 35.0% identity in 117 aa overlap. Also similar to CDS from other putative integrated plasmids in S.coelicolor e.g. SCE39.08c (EMBL:AL049573), Pra, regulatory protein (181 aa) (32.5% identity in 114 aa overlap). Part of a putative integrated plasmid similar to pSAM2 2 3 13 6 [Reporter]SCO0876 (16B22)_PCR/4805 [Gene]SCO0876/4383 NC_003888 SCM1.09c, unknown, len: 82 aa. 2 3 13 5 [Reporter]SCO3633 (17N18)_PCR/4804 [Gene]SCO3633/4382 NC_003888 SCH10.11, probable ABC transporter ATP-binding protein, len: 311 aa; similar to many e.g. TR:Q54406 (EMBL:X73633), tnrB2, from Streptomyces longisporoflavus, which is a component of an ABC transport system which confers resistance to the polyether-ionophore antibiotic tetronasin (300 aa), fasta scores; opt: 1323 z-score: 1477.2 E(): 0, 67.5% identity in 305 aa overlap. Also similar to TR:O86311 (EMBL:Z93777) M.tuberculosis hypothetical protein (311 aa) (63.0% identity in 305 aa overlap). Similar to many putative ABC transporter ATP-binding proteins from Streptomyces coelicolor e.g. SCD78.23 (EMBL:AL034355) putative ABC transporter ATP-binding protein (320 aa) (34.9% identity in 258 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, score 169.60, E-value 5.4e-47, PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 2 3 13 4 [Reporter]SCO6087 (18J18)_PCR/4803 [Gene]SCO6087/4381 NC_003888 SCBAC1A6.11, probable transport system integral membrane protein, len: 277 aa; similar to TR:Q9L1V0 (EMBL:AL138851) Streptomyces coelicolor putative sugar transporter inner membrane protein SCE59.03c, 281 aa; fasta scores: opt: 661 z-score: 794.5 E(): 0; 42.2% identity in 268 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and possible hydrophobic membrane spanning regions 2 1 2 13 [Reporter]SCO7160 (9B20)_PCR/538 [Gene]SCO7160/489 NC_003888 SC9A4.22c, conserved hypothetical protein, len: 277 aa; similar to TR:Q9RJW0 (EMBL:AL117387) Streptomyces coelicolor hypothetical 31.6 kDa protein SCF41.09, 294 aa; fasta scores: opt: 504 z-score: 582.4 E(): 5.7e-25; 38.1% identity in 281 aa overlap 2 3 13 3 [Reporter]SCO5355 (19F18)_PCR/4802 [Gene]SCO5355/4380 NC_003888 SCBAC5H2.24, thrC, threonine synthase, len: 352 aa; similar to SW:THRC_BACSU (EMBL:X04603) Bacillus subtilis threonine synthase (EC 4.2.99.2) ThrC, 352 aa; fasta scores: opt: 1373 z-score: 1545.9 E(): 0; 58.7% identity in 344 aa overlap. Contains Pfam match to entry PF00291 PALP, Pyridoxal-phosphate dependent enzyme and match to Prosite entry PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site 2 1 2 12 [Reporter]SCO1945 (10N16)_PCR/537 [Gene]SCO1945/488 NC_003888 SCC54.05c, tpiA, triosephosphate isomerase, len: 258 aa; member of a family conserved across many prokaryotes eg. SW:TPIS_BACSU tpi, triose phosphate isomerase from Bacillus subtilis (252 aa) fasta scores; opt: 779, z-score: 928.7, E(): 0, (51.2% identity in 250 aa overlap). Contains PS00171 Triosephosphate isomerase active site and Pfam match to entry PF00121 TIM, Triosephosphate isomerase, score 461.50, E-value 9.4e-149. 2 1 2 11 [Reporter]SCO4514 (11J16)_PCR/536 [Gene]SCO4514/487 NC_003888 SCD35.21c, possible integral membrane protein, len: 192 aa. Contains possible hydrophobic membrane spanning regions 2 1 2 10 [Reporter]SCO4271 (12F16)_PCR/535 [Gene]SCO4271/486 NC_003888 SCD95A.04, probable NADP-dependent alcohol dehydrogenase, len: 378 aa; similar to SW:ADH_MYCTU (EMBL:AL021287) Mycobacterium tuberculosis NADP-dependent alcohol dehydrogenase (EC 1.1.1.2) Adh, 346 aa; fasta scores: opt: 986 z-score: 1074.5 E(): 0; 45.0% identity in 349 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and match to Prosite entry PS00059 Zinc-containing alcohol dehydrogenases signature 2 1 2 9 [Reporter]SCO3433 (13B16)_PCR/534 [Gene]SCO3433/485 NC_003888 SCE9.40, conserved hypothetical protein, len: 258 aa; unknown function, C-terminus similar to N-terminal region of the clpC family of ATP-dependent Clp protease ATP-binding subunits e.g. TR:Q48760 (EMBL:U40604), mec, Listeria monocytogenes clpC ATPase (825 aa), fasta scores; opt: 272 z-score: 311.0 E(): 5.3e-10, 36.4% identity in 140 aa overlap. A hypothetical protein of similar length occurs in Mycobacterium tuberculosis; TR:P71964 (EMBL:Z80225) clpC family hypothetical protein (252 aa) (57.3% identity in 255 aa overlap). Contains probable helix-turn-helix motif at aa 62-83 (Score 1144, +3.08 SD) 2 1 2 8 [Reporter]SCO5012 (14N12)_PCR/533 [Gene]SCO5012/484 NC_003888 SCK15.14, possible integral membrane protein, len: 65 aa; similar to some other integral membrane proteins, e.g. TR:AAF40189 (EMBL:AF229646) Caulobacter crescentus PilA, 59 aa; fasta scores: opt: 84 z-score: 142.8 E(): 1.8; 36.2% identity in 47 aa overlap. Contains possible hydrophobic membrane spanning region 2 1 2 7 [Reporter]SCO5174 (15J12)_PCR/532 [Gene]SCO5174/483 NC_003888 SCP8.37, possible transferase, len: 593 aa; C-terminal region similar to TR:CAB92272 (EMBL:AL356595) Streptomyces coelicolor putative transferase SCC61A.27, 436 aa; fasta scores: opt: 233 z-score: 251.3 E(): 1.6e-06; 29.9% identity in 462 aa overlap. Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1. Also contains 3x conserved repeat unit: ARSSE 2 1 2 6 [Reporter]SCO0469 (16F12)_PCR/531 [Gene]SCO0469/482 NC_003888 SCF76.09, unknown, len: 173 aa. Weakly similar in parts to Bacillus subtilis TR:P96684 (EMBL; AB001488) YDFG protein (147 aa), fasta scores opt: 260 z-score: 329.6 E(): 5.3e-11 35.1% identity in 111 aa overlap. 2 1 2 5 [Reporter]SCO1065 (17B12)_PCR/530 [Gene]SCO1065/481 NC_003888 SCG22.11c, possible sugar transport sugar binding protein, len: 453 aa; similar to SW:LACE_AGRRD (EMBL:X66596) Agrobacterium radiobacter lactose-binding protein precursor LacE, 422 aa; fasta scores: opt: 273 z-score: 296.9 E(): 4.6e-09; 23.8% identity in 420 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and possible N-terminal region signal peptide sequence 2 1 2 4 [Reporter]SCO0332 (18N8)_PCR/529 [Gene]SCO0332/480 NC_003888 SCF12.11, possible transcriptional regulator, len: 206 aa; similar to many e.g. TR:AAD31815 (EMBL:AF129856) transcriptional repressor from Pseudomonas fluorescens (208 aa) fasta scores; opt: 275, z-score: 343.1, E(): 9.3e-12, 30.0% identity in 200 aa overlap. Contains probable helix-turn-helix motif (+5.38 SD) 45-66aa. 3 4 7 9 [Reporter]SCO3460 (13G17)_PCR/7195 [Gene]SCO3460/6559 NC_003888 SCE46.17, probable oxidoreductase, len: 505 aa; similar to SW:MERA_STAAU (EMBL:L29436) Staphylococcus aureus mercuric reductase (EC 1.16.1.1) MerA, 547 aa; fasta scores: opt: 891 z-score: 971.7 E(): 0; 37.4% identity in 452 aa overlap. Contains match to Prosite entry PS00076 Pyridine nucleotide-disulphide oxidoreductases class-I active site 3 4 7 7 [Reporter]SCO1151 (15O13)_PCR/7193 [Gene]SCO1151/6558 NC_003888 SCG8A.05c, possible integral membrane protein, len: 90aa; contains possible membrane-spanning hydrophobic regions. 3 4 7 6 [Reporter]SCO3838 (16K13)_PCR/7192 [Gene]SCO3838/6557 NC_003888 SCH69.08c, putative membrane protein, len: 412 aa; contains 7 x QG repeat 65-78aa. Contains possible hydrophobic membrane spanning region 3 4 7 5 [Reporter]SCO1164 (17G13)_PCR/7191 [Gene]SCO1164/6556 NC_003888 SCG8A.18, unknown, len: 108aa 3 4 7 4 [Reporter]SCO0614 (18C13)_PCR/7190 [Gene]SCO0614/6555 NC_003888 SCF55.38c, unknown, len: 94 aa 3 4 7 3 [Reporter]SCO6319 (19O9)_PCR/7189 [Gene]SCO6319/6554 NC_003888 SCIF3.21, possible lipoprotein, len: 260 aa; similar to TR:Q9XAG2 (EMBL:AL079356) Streptomyces coelicolor possible membrane protein SC6G9.20, 303 aa; fasta scores: opt: 984 z-score: 1092.9 E(): 0; 58.5% identity in 287 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 3 4 7 2 [Reporter]SCP1.124c (20G9)_PCR/7188 [Gene]SCP1.124c/6553 NC_003888 SCP1.124c, unknown, len: 79aa; 3 4 6 22 [Reporter]SCO5383 (1G5)_PCR/7186 [Gene]SCO5383/6552 NC_003888 2SC6G5.27c, possible ABC transporter, ATP-binding subunit, len: 260aa; similar to many eg. TR:Q53626 (EMBL:U43537) ATP-binding protein involved in mithramycin resistance from Streptomyces argillaceus (320 aa) fasta scores; opt: 901, z-score: 998.1, E():0, 59.6% identity in 225 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) and Prosite match to PS00211 ABC transporters family signature. 2 3 13 2 [Reporter]SCO5089 (20N14)_PCR/4801 [Gene]SCO5089/4379 NC_003888 SCBAC28G1.15, actIORF3, actinorhodin polyketide synthase acyl carrier protein, len: 86 aa; identical to previously sequenced SW:ACPA_STRCO (EMBL:X63449) Streptomyces coelicolor actinorhodin polyketide synthase acyl carrier protein ActI ORF3, 86 aa. Contains Pfam match to entry PF00550 pp-binding, Phosphopantetheine attachment site and match to Prosite entry PS00012 Phosphopantetheine attachment site 3 4 6 21 [Reporter]SCO0780 (2C5)_PCR/7185 [Gene]SCO0780/6551 NC_003888 3SCF60.12, probable zinc-binding oxidoreductase, len: 313 aa; identical to TR:O85702 (EMBL:AF072709) Streptomyces lividans putative oxidoreductase, 313 aa and similar to SW:QOR_HUMAN (EMBL:S58039) Homo sapiens quinone oxidoreductase (EC 1.6.5.5) CryZ, 329 aa; fasta scores: opt: 467 z-score: 501.6 E(): 2.1e-20; 32.5% identity in 329 aa overlap and to TR:CAB70647 (EMBL:AL137242) Streptomyces coelicolor putative zinc-binding oxidoreductase SC8F4.21, 339 aa; fasta scores: opt: 1311 z-score: 1209.2 E(): 0; 66.1% identity in 310 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and match to Prosite entry PS01162 Quinone oxidoreductase / zeta-crystallin signature 2 3 12 22 [Reporter]SCO1258 (1N10)_PCR/4799 [Gene]SCO1258/4378 NC_003888 2SCG18.05c, possible ABC transport system ATP-binding protein, len: 306 aa; similar to TR:CAB94566 (EMBL:AL359152) Streptomyces coelicolor putative ABC transporter ATP-binding protein 2SC6G5.39c, 346 aa; fasta scores: opt: 1018 z-score: 1166.6 E(): 0; 53.9% identity in 306 aa overlap and to SW:BCRA_BACLI (EMBL:L20573) Bacillus licheniformis bacitracin transport ATP-binding protein BcrA, 306 aa; fasta scores: opt: 594 z-score: 684.6 E(): 1.2e-30; 35.6% identity in 303 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 3 4 6 20 [Reporter]SCO1441 (3O1)_PCR/7184 [Gene]SCO1441/6550 NC_003888 SC6D7A.04c, ribAB, 3,4-dihydroxy-2-butanone 4-phosphate synthase, len: 429 aa; similar to SW:GCH2_CORAM (EMBL:AB003693) Corynebacterium ammoniagenes 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 3.5.4.25) RibA, 423 aa; fasta scores: opt: 1462 z-score: 1644.1 E(): 0; 56.2% identity in 404 aa overlap and C-terminal domain similar to TR:O88011 (EMBL:AL031107) Streptomyces coelicolor hypothetical protein SC5A7.05, 221 aa; fasta scores: opt: 772 z-score: 746.1 E(): 0; 55.8% identity in 208 aa overlap. Contains Pfam match to entry PF00926 DHBP_synthase, 3,4-dihydroxy-2-butanone 4-phosphate synthase and PF00925 GTP_cyclohydro2, GTP cyclohydrolase II 2 3 12 21 [Reporter]SCO4855 (2J10)_PCR/4798 [Gene]SCO4855/4377 NC_003888 SC5G8.23c, dhsB, probable succinate dehydrogenase iron-sulfur subunit, len: 257aa; strongly similar to many eg. SW:P07014 (DHSB_ECOLI) succinate dehydrogenase iron-sulfur protein from Escherichia coli (238 aa) fasta scores; opt: 729, z-score: 839.1, E(): 0, 44.3% identity in 237 aa overlap. Contains Pfam match to entry PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains, Pfam match to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains and Prosite match to PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. 2 3 12 20 [Reporter]SCO4078 (3F10)_PCR/4797 [Gene]SCO4078/4376 NC_003888 SCD25.14, purQ, phosphoribosyl formylglycinamidine synthase I (EC 6.3.5.3), len: 226 aa, Highly similar to many e.g. Bacillus subtilis SW:PURQ_BACSU (EMBL; J02732) phosphoribosylformylglycinamidine synthase I (EC 6.3.5.3) (FGAM synthase I) (227 aa), fasta scores opt: 650 z-score: 766.2 E(): 0 47.5% identity in 219 aa overlap and Mycobacterium tuberculosis TR:P71841 (EMBL; Z80226) putative phosphoribosylformylglycinamidine synthase I (EC 6.3.5.3) (FGAM synthase I) MTCY369.32 (224 aa), fasta scores opt: 924 z-score: 1085.2 E(): 0 65.0% identity in 220 aa overlap. Contains a Prosite hit to PS00442 Glutamine amidotransferases class-I active site. 2 3 12 19 [Reporter]SCO5548 (4B10)_PCR/4796 [Gene]SCO5548/4375 NC_003888 SC1C2.29, unknown, len: 131 aa; similar to M. tuberculosis hypothetical protein TR:O07205 (EMBL:Z96072) MTCY05A6.25 (142 aa), fasta scores; opt: 149 z-score: 314.1 E(): 3e-10, 34.7% identity in 121 aa overlap 2 3 12 18 [Reporter]SCO2199 (5N6)_PCR/4795 [Gene]SCO2199/4374 NC_003888 SC3H12.07, possible integral membrane protein, len: 89 aa; identical to previously sequenced TR:CAB51283 (EMBL:AL096872) Streptomyces coelicolor hypothetical protein SC5F7.02, 89 aa. Contains possible hydrophobic membrane spanning regions 2 3 12 17 [Reporter]SCO5818 (6J6)_PCR/4794 [Gene]SCO5818/4373 NC_003888 SC5B8.08, probable ABC transporter, len: 744 aa; N-t erminus is similar to several ABC transporters e.g. NODI_RH IGA P50332 from Rhizobium galegae nodulation atp-binding pr otein (347 aa), fasta scores; opt: 285 z-score: 225.2 E():2 .4e-05, 34.5% identity in 197 aa overlap, and contains PS00 017 ATP/GTP-binding site motif A (P-loop) and Pfam match to entry ABC_tran PF00005, ABC transporters, score 135.08. Th e C-terminus (approx aa 500-744) contains several probable transmembrane domains. In the centre is a repetitive region containing 10 copies of a degenerate 7 aa Proline-rich rep eat. 1 2 19 7 [Reporter]SCO5150 (16M3)_PCR/2418 [Gene]SCO5150/2199 NC_003888 SCP8.13, putative membrane protein, len: 151 aa; similar to TR:O69415 (EMBL:AJ005830) Escherichia coli Sec-independent protein translocase TatB, 171 aa; fasta scores: opt: 169 z-score: 186.8 E(): 0.0061; 23.6% identity in 161 aa overlap. High in aspartate, glutamate and serine amino acid residues. Highly similar to TR:Q9EWB3 (EMBL:AJ292256) Streptomyces lividans twin arginine translocation complex component TatB 158 aa; fasta scores: opt: 1001 Z-score: 1020.1 E(): 3.5e-49; 97.516% identity in 161 aa overlap. Contains possible hydrophobic membrane spanning region 2 3 12 16 [Reporter]SCO7272 (7F6)_PCR/4793 [Gene]SCO7272/4372 NC_003888 SC5H1.20, possible isomerase, len: 127 aa; weakly similar to SW:SDIS_PSEPU (EMBL:L13127), ksI, Pseudomonas putida steroid delta-isomerase (131 aa), fasta scores; opt: 109 z-score: 150.4 E(): 0.47, 26.0% identity in 131 aa overlap 1 2 19 6 [Reporter]SCO7629 (17I3)_PCR/2417 [Gene]SCO7629/2198 NC_003888 SC10F4.02, spaA, probable starvation sensing protein, len: 413 aa; identical to previously sequenced TR:P95726 (EMBL:X94190) Streptomyces coelicolor spaA gene, 413 aa. Contains Pfam match to entry PF01188 MR_MLE, Mandelate racemase / muconate lactonizing enzyme family and match to Prosite entry PS00908 Mandelate racemase / muconate lactonizing enzyme family signature 1 2 3 12 15 [Reporter]SCO4749 (8B6)_PCR/4792 [Gene]SCO4749/4371 NC_003888 SC6G4.27c, possible secreted protein, len: 184 aa; similar to TR:Q9KZZ6 (EMBL:AL353815) Streptomyces coelicolor putative secreted protein SCD6.08, 189 aa; fasta scores:opt: 495 Z-score: 540.3 E(): 1.8e-22; 48.066% identity in 181 aa overlap. Contains possible N-terminal signal sequence 2 1 2 3 [Reporter]SCP1.167 (19J8)_PCR/528 [Gene]SCP1.167/479 NC_003888 SCP1.167, possible membrane protein, len: 227aa; weakly similar to TR:Q9L0S0 (EMBL:AL160312) hypothetical protein from Streptomyces coelicolor (239 aa) fasta scores; opt: 202, z-score: 221.4, E(): 7.3e-05, 27.2% identity in 235 aa overlap. Note possible alternative downstream start codons. 1 2 19 5 [Reporter]SCO0351 (18E3)_PCR/2416 [Gene]SCO0351/2197 NC_003888 SCF41.10, unknown, len: 491 aa. 2 3 12 14 [Reporter]SCO2653 (9N2)_PCR/4791 [Gene]SCO2653/4370 NC_003888 SC8E4A.23, hypothetical protein, len: 221 aa; similar to SW:Y089_MYCTU (EMBL:Z74410) Mycobacterium tuberculosis putative methyltransferase RV0089 (EC 2.1.1.-) MTCY251.07, 197aa, fasta scores: opt: 258 z-score: 309.5 E(): 7.9e-10; 29.6% identity in 203 aa overlap and to TR:AAF09618 (EMBL:AE001866) Deinococcus radiodurans methyltransferase, putative DR0026, 269 aa; fasta scores: opt: 188 z-score: 226.5 E(): 3.3e-05; 32.7% identity in 168 aa overlap 2 1 2 2 [Reporter]SCP1.103 (20B8)_PCR/527 [Gene]SCP1.103/478 NC_003888 SCP1.103, unknown, len: 177aa; weakly similar to TR:Q9L158 (EMBL:AL158060) hypothetical protein from Streptomyces coelicolor (132 aa) fasta scores; opt: 125, z-score: 165.0, E(): 0.1, 33.3% identity in 129 aa overlap. 1 2 19 4 [Reporter]SCO6086 (19A3)_PCR/2415 [Gene]SCO6086/2196 NC_003888 SCBAC1A6.10, probable transport system integral membrane protein, len: 315 aa; similar to TR:P73352 (EMBL:D90905) Synechocystis sp. lactose transport system permease protein LacF, 298 aa; fasta scores: opt: 630 z-score: 734.0 E(): 0; 38.6% identity in 298 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and possible hydrophobic membrane spanning regions 2 1 1 22 [Reporter]SCO5202 (1B4)_PCR/525 [Gene]SCO5202/477 NC_003888 2SC3B6.26, possible secreted protein, len: 364 aa; similar to TR:O53340 (EMBL:AL021646) Mycobacterium tuberculosis hypothetical 35.5. kD protein, 340 aa; fasta scores: opt: 430 z-score: 467.4 E(): 1.4e-18; 41.3% identity in 361 aa overlap. Contains possible N-terminal signal peptide sequence 1 2 19 3 [Reporter]SCO5345 (19M23)_PCR/2414 [Gene]SCO5345/2195 NC_003888 SCBAC5H2.14, conserved hypothetical protein, len: 183 aa; highly similar to plasmid borne TR:CAA06454 (EMBL:AJ005260) Streptomyces ambofaciens unknown ORF183, 183 aa; fasta scores: opt: 1018 z-score: 1225.2 E(): 0; 81.4% identity in 183 aa overlap. Contains Pfam match to entry PF00990 DUF9, Domain of unknown function DUF9 and a TTA leucine codon, possible target for bldA regulation 2 1 1 21 [Reporter]SCO4918 (1N24)_PCR/524 [Gene]SCO4918/476 NC_003888 SCK13.10c, conserved hypothetical protein, len: 145 aa; similar to TR:O53356 (EMBL:AL021841) Mycobacterium tuberculosis hypothetical 17.5 kDa protein MTV016.03, 159 aa; fasta scores: opt: 621 z-score: 794.6 E(): 0; 60.4% identity in 149 aa overlap 1 2 19 2 [Reporter]SCO3977 (20E23)_PCR/2413 [Gene]SCO3977/2194 NC_003888 SCBAC25E3.14, possible protease (putative secreted protein), len: 519aa: similar to many eg. TR:Q9FBK9 (EMBL:AL390975) putative protease SCP8.12 from Streptomyces coelicolor (542 aa) fasta scores; opt: 1265, Z-score: 796.4, 43.636% identity (47.431% ungapped) in 550 aa overlap and TR:O06291 (EMBL:Z98260) HtrA from Mycobacterium tuberculosis (549 aa) fasta scores; opt: 614, Z-score: 396.6, 34.870% identity (37.826% ungapped) in 499 aa overlap. Contains Pfam match to entry PF00089 trypsin, Trypsin and Pfam match to entry PF00595 PDZ, PDZ domain (Also known as DHR or GLGF). Contains possible N-terminal region signal peptide sequence also possible hydrophobic membrane spanning region 2 1 1 20 [Reporter]SCO4567 (2J24)_PCR/523 [Gene]SCO4567/475 NC_003888 SCD16A.16c, nuoF, NADH dehydrogenase subunit, len: 449 aa; similar to many e.g. TR:P95176 (EMBL:Z83867) NuoF, NADH dehydrogenase subunit from Mycobacterium tuberculosis (445 aa) fasta scores; opt: 2314, z-score: 2659.4, E(): 0, (76.3% identity in 434 aa overlap) and SW:NUOF_ECOLI, NADH dehydrogenase subunit NuoF from Escherichia coli (445 aa) fasta scores; opt: 1346, z-score: 1547.8, E(): 0, (48.1% identity in 405 aa overlap). Contains Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00645 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. 1 2 18 22 [Reporter]SCO1123 (1E19)_PCR/2411 [Gene]SCO1123/2193 NC_003888 2SCG38.16, unknown, len: 132 aa 2 1 1 19 [Reporter]SCO6464 (3F24)_PCR/522 [Gene]SCO6464/474 NC_003888 SC1A11.01c; partial CDS, possible SIR2 family transcriptional regulator, len: 86 aa; overlapping N-terminal region of SC9B5.31c, from Streptomyces coelicolor cosmid 9B5. Similar to hypothetical proteins and proposed SIR2 family transcriptional regulators eg. TR:O28597 (EMBL:AE000987) proposed transcriptional regulatory protein, SIR2 family from Archaeoglobus fulgidus (245 aa) fasta scores; opt: 221, z-score: 297.7, E(): 2.7e-09, (36.2% identity in 185 aa overlap).,SC9B5.31c; partial CDS, possible SIR2 family transcriptional regulator, len: 190aa; overlapping C-terminal region of SC1A11.01c, from Streptomyces coelicolor cosmid 1A11. Similar to hypothetical proteins and proposed SIR2 family transcriptional regulators eg. TR:O28597 (EMBL:AE000987) proposed transcriptional regulatory protein, SIR2 family from Archaeoglobus fulgidus (245 aa) fasta scores; opt: 370, z-score: 428.3, E(): 1.4e-16, (36.2% identity in 185 aa overlap). 1 2 18 21 [Reporter]SCO4761 (2A19)_PCR/2410 [Gene]SCO4761/2192 NC_003888 SC6G4.39, groES, 10 kD chaperonin cpn10, len: 102 aa ; identical to CH10_STRCO (102 aa) and highly similar to ma ny e.g. CH10_BACSU (94 aa), fasta scores; opt: 353 z-score: 567.4 E(): 2.4e-24, 54.3% identity in 92 aa overlap. Cont ains PS00681 Chaperonins cpn10 signature and Pfam match to entry PF00166 cpn10, Chaperonins 10 Kd subunit, score 200.6 0, E-value 2.4e-56 2 1 1 18 [Reporter]SCO6200 (4B24)_PCR/521 [Gene]SCO6200/473 NC_003888 SC2G5.21c, unknown, len: 230aa 1 2 18 20 [Reporter]SCO6816 (3M15)_PCR/2409 [Gene]SCO6816/2191 NC_003888 SC1A2.25c, possible ABC transporter binding lipoprotein, len: 290 aa. Similar to several including: Escherichia coli SW:PSTS_ECOLI (EMBL:K01992) phosphate-binding protein precursor (346 aa), fasta scores opt: 158 z-score: 176.7 E(): 0.019 25.9% identity in 259 aa overlap and Methanobacterium thermoautotrophicum TR:O27760 (EMBL:AE000929) phosphate-binding protein, PstS (271 aa), fasta scores opt: 510 z-score: 554.5 E(): 1.8e-23 34.9% identity in 281 aa overlap. Contains an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site, a Pfam match to entry PF01449 PstS, Phosphate-binding protein and a possible N-terminal signal sequence. 2 1 1 17 [Reporter]SCO7045 (5N20)_PCR/520 [Gene]SCO7045/472 NC_003888 SC4G1.11, hypothetical protein, len: 114 aa; similar to TR:O59172 (EMBL:AP000006) Pyrococcus horikoshii hypothetical 14.9 kDa protein PH1503, 130 aa; fasta scores: opt: 302 z-score: 405.0 E(): 4.3e-15; 41.0% identity in 100 aa overlap 1 2 18 19 [Reporter]SCO7214 (4I15)_PCR/2408 [Gene]SCO7214/2190 NC_003888 SC2H12.13c, possible integral membrane protein, len: 355 aa. Similar to several proteins of unknown function e.g. Streptomyces coelicolor TR:Q9S290(EMBL:AL096849) putative membrane protein, SCI11.29 (354 aa), fasta scores opt: 1082 z-score: 1169.9 E():0 47.4% identity in 350 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 2 1 1 16 [Reporter]SCO7304 (6J20)_PCR/519 [Gene]SCO7304/471 NC_003888 SC5F8.14, unknown, len: 557 aa. Highly similar to several proteins of undefined function including: Mycobacterium tuberculosis TR:P71838(EMBL:Z80226) (566 aa), fasta scores opt: 2386 z-score: 2598.2 E():0 63.1% identity in 553 aa overlap and Deinococcus radiodurans TR:Q9RYS0(EMBL:AE001863) (554 aa), fasta scores opt: 2249 z-score: 2449.3 E():0 60.2% identity in 555 aa overlap. 2 1 1 15 [Reporter]SCO7413 (7F20)_PCR/518 [Gene]SCO7413/470 NC_003888 SC6D11.09, possible oxidoreductase, len: 343 aa. Highly similar to many putative oxidoreductases including: Mycobacterium tuberculosis TR:O53398(EMBL:AL021897) oxidoreductase (301 aa), fasta scores opt: 405 z-score: 443.2 E(): 2.9e-17 36.2% identity in 257 aa overlap. Contains a Pfam match to entry PF00106 adh_short, short chain dehydrogenase and a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature. 3 4 6 18 [Reporter]SCO7099 (5G1)_PCR/7182 [Gene]SCO7099/6549 NC_003888 SC3A4.25c, possible RNA polymerase sigma factor, len: 300 aa. Similar to many putative sigma factors from Streptomyces coelicolor e.g. TR:CAB60158 (EMBL:AL132824) SCAH10.04C (322 aa), fasta scores opt: 427 z-score: 505.7 E(): 1e-20 36.5% identity in 307 aa overlap and TR:CAB59711 (EMBL:AL132707) SCF51.13C (298 aa), fasta scores opt: 445 z-score: 527.2 E(): 6.6e-22 38.6% identity in 298 aa overlap. 3 4 6 17 [Reporter]SCO7330 (6C1)_PCR/7181 [Gene]SCO7330/6548 NC_003888 SC4G10.09, possible membrane protein, len: 78 aa. Contains a possible membrane spanning hydrophobic domain. 3 4 6 16 [Reporter]SCO7318 (6O21)_PCR/7180 [Gene]SCO7318/6547 NC_003888 SC5F8.28c, possible membrane protein, len: 752 aa; similar to TR:Q9AJZ2 (EMBL:AL512944) Streptomyces coelicolor putative integral membrane protein SC8D11.27c, 753 aa; fasta scores: opt: 773 Z-score: 794.0 E(): 1.4e-36; 37.281% identity in 743 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. Note the predicted product of this CDS is rich in the amino acid Leucine 3 4 6 15 [Reporter]SCO6954 (7K21)_PCR/7179 [Gene]SCO6954/6546 NC_003888 SC6F7.07, probable NADH dehydrogenase I complex, subunit, len: 967 aa. Similar to many including: Escherichia coli SW:NUOL_ECOLI(EMBL:X68301) NADH dehydrogenase I chain L (EC 1.6.5.3) (613 aa), fasta scores opt: 717 z-score: 781.4 E():0 32.7% identity in 517 aa overlap and Rhodobacter capsulatus TR:O68034(EMBL:AF010496) NADH-quinone oxidoreductase chain 5 (EC 1.6.5.3) (961 aa), fasta scores opt: 1059 z-score: 1151.4 E():0 35.0% identity in 989 aa overlap. Also weakly similar to CDS SC6F7.09 also carried on this cosmid, fasta scores opt: 476 z-score: 387.6 E(): 4.5e-16 28.2% identity in 444 aa overlap. Contains a Pfam match to entry PF00361 oxidored_q1, NADH-Ubiquinone/plastoquinone (complex I), various chains. Also contains multiple possible membrane spanning hydrophobic domains. 3 4 6 14 [Reporter]SCO6891 (8G21)_PCR/7178 [Gene]SCO6891/6545 NC_003888 SC7F9.43, unknown, len: 160 aa 3 4 6 13 [Reporter]SCO7169 (9C21)_PCR/7177 [Gene]SCO7169/6544 NC_003888 SC9A4.31c, unknown, len: 144 aa. Highly hydrophilic. Contains 2x conserved repeat AGSGNT and high content in G+C (78.24%) 3 4 6 12 [Reporter]SCO1955 (10O17)_PCR/7176 [Gene]SCO1955/6543 NC_003888 SCC54.15c, possible iron sulphur binding membrane lipoprotein, len: 141aa; similar to many of this type eg. SW:UCRI_CHLLT cytochrome B6-F iron-sulphur subunit, petC from chlorobium limicola (180 aa) fasta scores; opt: 197, z-score: 225.2, E(): 3.1e-05, (33.3% identity in 108 aa overlap). Contains possible N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 3 4 6 11 [Reporter]SCO4523 (11K17)_PCR/7175 [Gene]SCO4523/6542 NC_003888 SCD35.30, possible integral membrane protein, len: 197 aa. Contains possible hydrophobic membrane spanning regions 2 3 12 13 [Reporter]SCO6256 (10J2)_PCR/4790 [Gene]SCO6256/4369 NC_003888 SCAH10.21, possible transcriptional regulator, len: 245 aa; similar to SW:YURK_BACSU (EMBL:Z99120) Bacillus subtilis hypothetical transcriptional regulator in hom-MrgA intergenic region YurK, 242 aa; fasta scores: opt: 333 z-score: 402.6 E(): 4.7e-15; 26.8% identity in 235 aa overlap and to SW:FARR_ECOLI (EMBL:X15790) Escherichia coli, response regulator FarR, 240 aa; fasta scores: opt: 270 z-score: 328.3 E(): 6.5e-11; 26.1% identity in 226 aa overlap. Contains match to Pfam entry PF00392 gntR, bacterial regulatory proteins, gntR family and a possible helix-turn-helix motif at residues 18458..18523 3 4 6 10 [Reporter]SCO4281 (12G17)_PCR/7174 [Gene]SCO4281/6541 NC_003888 SCD95A.14, hypothetical protein, len: 553 aa; similar to TR:AAF10046 (EMBL:AE001906) Deinococcus radiodurans sensory Box/GgdEF family protein, 805 aa; fasta scores: opt: 280 z-score: 304.9 E(): 1.6e-09; 28.6% identity in 364 aa overlap. Contains Pfam match to entry PF01590 GAF, GAF domain, PF00989 PAS, PAS domain, PF00785 PAC, PAC motif and PF00990 DUF9, Domain of unknown function. Contains also possible coiled-coil region at aprox. residues 197..214. High content in alanine and arginine amino acid residues 2 3 12 12 [Reporter]SCO2715 (11F2)_PCR/4789 [Gene]SCO2715/4368 NC_003888 SCC61A.36, unknown, len: 432aa; 3 4 6 9 [Reporter]SCO1040 (13C17)_PCR/7173 [Gene]SCO1040/6540 NC_003888 SCG20A.20c, probable DNA repair protein, len: 216 aa; similar to SW:ALKB_ECOLI (EMBL:J02607) Escherichia coli DNA repair protein AlkB, 216 aa; fasta scores: opt: 302 z-score: 361.8 E(): 1.1e-12; 34.3% identity in 207 aa overlap 2 3 12 11 [Reporter]SCO4454 (12B2)_PCR/4788 [Gene]SCO4454/4367 NC_003888 SCD6.32c, possible transcriptional regulator, len: 200 aa; similar to TR:O86514 (EMBL:AL031124) Streptomyces coelicolor putative transcriptional regulator SC1C2.13, 194 aa; fasta scores: opt: 342 z-score: 413.0 E(): 1.5e-15; 39.0% identity in 172 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 35..56 (+2.77 SD) 2 3 12 10 [Reporter]SCO2829 (12N22)_PCR/4787 [Gene]SCO2829/4366 NC_003888 SCE20.03, amino acid ABC transporter protein, integral membrane component, len: 233 aa. Highly similar to many including: Escherichia coli SW:GLNP_ECOLI(EMBL:X14180) glutamine transport system permease protein GlnP (219 aa), fasta scores opt: 377 z-score: 445.7 E(): 1.9e-17 27.8% identity in 212 aa overlap and Rhizobium sp. (strain NGR234) SW:Y4TF_RHISN(EMBL:AE000098) probable amino-acid ABC transporter permease protein Y4TF (238 aa), fasta scores opt: 570 z-score: 667.5 E(): 8.5e-30 45.1% identity in 204 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Also contains a possible N-terminal signal sequence and possible membrane spanning hydrophobic domains. 2 3 12 9 [Reporter]SCO3810 (13J22)_PCR/4786 [Gene]SCO3810/4365 NC_003888 SCGD3.11c, probable gntR-family transcriptional regulator, len: 216 aa; similar to many e.g. SW:GNTR_BACSU (EMBL:J02584), GntR, Bacillus subtilis gluconate operon transcriptional repressor (243 aa), fasta scores; opt: 228 z-score: 274.3 E(): 5.9e-08, 28.5% identity in 186 aa overlap. Also similar to others from S.coelicolor (EMBL:AL034443) S.coelicolor probable transcriptional regulator (225 aa) (33.6% identity in 214 aa overlap). Contains probable helix-turn-helix motif at aa 34-55 (Score 991, +2.56 SD). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and PS00043 Bacterial regulatory proteins, gntR family signature 2 3 12 8 [Reporter]SCO0678 (14F22)_PCR/4785 [Gene]SCO0678/4364 NC_003888 SCF91.38c, unknown, len: 57 aa. Similar to Bacillus subtilis TR:P70964(EMBL:Z81356) hypothetical 6.8 KD protein (62 aa), fasta scores opt: 104 z-score: 152.5 E(): 0.4 33.3% identity in 57 aa overlap. 2 3 12 7 [Reporter]SCO3928 (15B22)_PCR/4784 [Gene]SCO3928/4363 NC_003888 SCQ11.11, thiC, probable thiamine biosynthesis protein, len: 612 aa; similar to many e.g. SW:THIC_ECOLI (EMBL:M88701), ThiC, Escherichia coli protein required for the synthesis of the hydroxymethylpyrimidine moiety of thiamine (631 aa), fasta scores; opt: 2216 z-score: 2538.6 E(): 0, 60.7% identity in 639 aa overlap. The C-terminal 49 residues are repeated (with slight divergence) as SCD78.05, thiC' probable thiamine biosynthesis protein thiC, partial CDS (43 aa) 1 2 18 18 [Reporter]SCO2391 (5E15)_PCR/2407 [Gene]SCO2391/2189 NC_003888 SC4A7.19c, hypothetical protein, len: 164 aa; identical to previously sequenced TR:Q54209 (EMBL:L43074) Streptomyces glaucescens ORF5, 164 aa and similar to TR:O53530 (EMBL:AL021925) Mycobacterium tuberculosis hypothetical 18.9 kD protein, 177 aa; fasta scores: opt: 489 z-score: 628.5 E(): 1.3e-27; 42.6% identity in 162 aa overlap 2 3 12 6 [Reporter]SCO0580 (16N18)_PCR/4783 [Gene]SCO0580/4362 NC_003888 SCF55.04c, possible glycerone kinase, len: 593 aa; similar to SW:DHAK_YEAST (EMBL:D50617) Saccharomyces cerevisiae dihydroxyacetone kinase 2 (EC 2.7.1.29) (glycerone kinase) Dak2, 591 aa; fasta scores: opt: 646 z-score: 660.2 E(): 2.1e-29; 29.9% identity in 561 aa overlap 1 2 18 17 [Reporter]SCO7767 (6A15)_PCR/2406 [Gene]SCO7767/2188 NC_003888 SC5E9.15c, possible DNA-binding protein, len: 275 aa; similar to many e.g. TR:Q9RD19 (EMBL:AL133422) putative DNA-binding protein from Streptomyces coelicolor (279 aa) fasta scores; opt: 510, z-score: 601.1, E(): 5.1e-26, 36.4% identity in 275 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and a possible helix-turn-helix motif at residues 40..61 (+3.20 SD) 2 3 12 5 [Reporter]SCO5037 (17J18)_PCR/4782 [Gene]SCO5037/4361 NC_003888 SCK7.10c, unknown, len: 101 aa. Highly hydrophilic. Also high in G+C content (80.52 %) 2 1 1 14 [Reporter]SCO6878 (8B20)_PCR/517 [Gene]SCO6878/469 NC_003888 SC7F9.30c, possible ATP-binding protein, len: 507 aa. Highly similar to Streptomyces coelicolor TR:Q9X873(EMBL:AL049661) putative ATP-binding protein, SCE134.18 (489 aa), fasta scores opt: 2405 z-score: 2548.3 E(): 0 73.5% identity in 490 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 1 2 18 16 [Reporter]SCO5660 (7M11)_PCR/2405 [Gene]SCO5660/2187 NC_003888 SC6A9.07, probable secreted peptidase, len: 386 aa; similar to e.g. DAC_STRSQ D-alanyl-D-alanine carboxypeptidase precursor (406 aa), fasta scores; oopt: 544 z-score: 500.9 E(): 1.2e-20, 33.2% identity in 365 aa overlap. Contains N-terminal signal sequence and PS00017 ATP /GTP-binding site motif A (P-loop) 2 3 12 4 [Reporter]SCO3219 (18F18)_PCR/4781 [Gene]SCO3219/4360 NC_003888 SCE8.12c, possible lipase (putative secreted protein), len: 391 aa; weak similarity to many eukaryotic lipases e.g. SW:PAFA_CAVPO platelet-activating factor-acetylhydrolase from guinea pig (Cavia porcellus) (436 aa) fasta scores; opt: 153, z-score: 161.7, E(): 0.11, (22.9% identity in 319 aa overlap). Contains PS00120 Lipases, serine active site. Contains possible N-terminal region signal peptide sequence 2 1 1 13 [Reporter]SCO6126 (9N16)_PCR/516 [Gene]SCO6126/468 NC_003888 SC9B2.13c, unknown, len: 104 aa 1 2 18 15 [Reporter]SCO6013 (8I11)_PCR/2404 [Gene]SCO6013/2186 NC_003888 SC1C3.01, probable 1-deoxyxylulose-5-phosphate synthase, partial CDS, len: >341 aa; overlaps and extends SC7B7.10. Similar to many members of the transketolase family e.g. DXS_MYCTU probable 1-deoxyxylulose-5-phosphate synthase (638 aa), fasta scores; opt: 1288 z-score: 1753.5 E(): 0, 59.1% identity in 323 aa overlap. Contains Pfam match to entry transketolase PF00456, Transketolase, score 32.69,SC7B7.10, unknown, partial CDS, len: >352 aa, similar to many members of the transketolase family eg. TR:O07184 (EMBL:Z96072) MTCY05A6.03C hypothetical protein from M. tuberculosis (638 aa), fasta scores; opt: 1365 z-score: 1798.3 E(): 0, 58.7% identity in 351 aa overlap. Contains PS00801 Transketolase signature 1 2 1 1 12 [Reporter]SCO1941 (10J16)_PCR/515 [Gene]SCO1941/467 NC_003888 SCC54.01c, partial CDS, possible intergral membrane protein, len: >183aa; contains possible membrane spanning hydrophobic regions.,SCC22.23c, partial CDS, possible integral membrane protein, len: >178aa; forms the C-terminal portion of TR:CAB38131 (EMBL:AL035591) partial CDS, possible integral membrane protein on the end of cosmid C54 from Streptomyces coelicolor. 1 2 18 14 [Reporter]SCO5517 (9E11)_PCR/2403 [Gene]SCO5517/2185 NC_003888 SC8D9.29, possible transcriptional regulator, len: 196 aa; some similarity to many e.g. TR:O34381 (EMBL:Z99112) PksA, transcriptional regulator, involved in regulation of the polyketide synthase operon in Bacillus subtilis (205 aa) fasta scores; opt: 108, z-score: 141.0, E(): 1.5, (32.1% identity in 56 aa overlap) and TR:O86510 (EMBL:AL031124) putative transcriptional regulator from Streptomyces coelicolor (200 aa) fasta scores; opt: 135, z-score: 173.7, E(): 0.023, (30.9% identity in 139 aa overlap). 2 1 1 11 [Reporter]SCO4349 (11F16)_PCR/514 [Gene]SCO4349/466 NC_003888 SCD19.04c, hypothetical protein, len: 571 aa; similar to some plasmid derived hypothetical proteins, e.g. TR:O85872 (EMBL:AF079317) Sphingomonas aromaticivorans hypothetical 65.3 kDa protein (plasmid pNL1), 595 aa; fasta scores: opt: 344 z-score: 399.6 E(): 9.4e-15; 26.7% identity in 561 aa overlap. Contains two TTA leucine codons, possible targets for bldA regulation 1 2 18 13 [Reporter]SCO3794 (10A11)_PCR/2402 [Gene]SCO3794/2184 NC_003888 SCAC2.02c, probable glycosyl transferase, len: 257 aa; similar to TR:BAB07380 (EMBL:AP001519) Bacillus halodurans teichuronic acid biosynthesis TuaG, 257 aa; fasta scores: opt: 624 z-score: 745.5 E(): 0; 41.3% identity in 252 aa overlap. Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases 2 1 1 10 [Reporter]SCO4257 (12B16)_PCR/513 [Gene]SCO4257/465 NC_003888 SCD8A.30, possible hydrolytic protein, len: 480 aa; similar to TR:Q9ZBF7 (EMBL:AL035206) Streptomyces coelicolor putative hydrolytic protein SC9B5.23c, 464 aa; fasta scores: opt: 1760 z-score: 1669.0 E(): 0; 63.0% identity in 487 aa overlap. Contains possible hydrophobic membrane spanning region 1 2 18 12 [Reporter]SCO4389 (11M7)_PCR/2401 [Gene]SCO4389/2183 NC_003888 SCD10.21, possible acetyltransferase, len: 291 aa; N-terminal domain similar to TR:Q9S2T6 (EMBL:AL096884) Streptomyces coelicolor conserved hypothetical protein SC4G6.18c, 134 aa; fasta scores: opt: 259 z-score: 321.5 E(): 2e-10; 39.8% identity in 128 aa overlap and C-terminal domain similar to TR:Q9S2P7 (EMBL:AL096872) Streptomyces coelicolor putative acetyltransferase SC5F7.12c, 169 aa; blastp scores: Score = 90 (31.7 bits), Expect = 0.043, P = 0.042 Identities = 37/125 (29%), Positives = 53/125 (42%). Contains Pfam matches to entries PF01042 UPF0076, Domain of unknown function and PF00583 Acetyltransf, Acetyltransferase (GNAT) family 2 1 1 9 [Reporter]SCO3456 (13N12)_PCR/512 [Gene]SCO3456/464 NC_003888 SCE46.13c, possible secreted protein, len: 135 aa; similar to TR:CAC41457 (EMBL:AL591782) Rhizobium meliloti conserved hypothetical protein SMC02589, 408 aa; fasta scores: opt: 927 Z-score: 1029.9 E(): 9.8e-50; 37.198% identity in 414 aa overlap. Contains possible N-terminal region signal peptide sequence 1 2 18 11 [Reporter]SCO3959 (12I7)_PCR/2400 [Gene]SCO3959/2182 NC_003888 SCD78.26, possible integral membrane protein, len: 239 aa, possible membrane spanning regions 2 1 1 8 [Reporter]SCO1803 (14J12)_PCR/511 [Gene]SCO1803/463 NC_003888 SCI33.02, possible oxidoreductase, len: 231 aa; similar to TR:Q9KAS7 (EMBL:AP001514) Bacillus halodurans 3-oxoacyl-(acyl-carrier-protein) reductase BH2210, 244 aa; fasta scores: opt: 369 z-score: 399.8 E(): 1e-14; 35.3% identity in 235 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 1 2 18 10 [Reporter]SCO3082 (13E7)_PCR/2399 [Gene]SCO3082/2181 NC_003888 SCE25.23, unknown, len: 76 aa 2 1 1 7 [Reporter]SCO1024 (15F12)_PCR/510 [Gene]SCO1024/462 NC_003888 SCG20A.04, hypothetical protein, len: 833 aa; highly similar in its C-terminal domain to SW:SP5K_BACSU (EMBL:X59412) Bacillus subtilis stage V sporulation protein K SpoVK, 322 aa; fasta scores: opt: 699 z-score: 720.9 E(): 1.1e-32; 42.6% identity in 265 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 2 18 8 [Reporter]SCO1799 (15M3)_PCR/2397 [Gene]SCO1799/2180 NC_003888 SCI5.07, unknown, len: 259 aa; similar to SW:YS07_MYCTU hypothetical protein from Mycobacterium tuberculosis (302 aa) fasta scores; opt: 131, z-score: 146.5, E(): 0.79, (32.3% identity in 279 aa overlap). 2 1 1 6 [Reporter]SCO0281 (16B12)_PCR/509 [Gene]SCO0281/461 NC_003888 SCF85.09c, unknown, len: 122 aa. 2 1 1 5 [Reporter]SCO3371 (17N8)_PCR/508 [Gene]SCO3371/460 NC_003888 SCE94.22, unknown, len: 239aa; 3 4 6 8 [Reporter]SCO0171 (14O13)_PCR/7172 [Gene]SCO0171/6539 NC_003888 SCJ1.20, conserved hypothetical protein, len: 438 aa. Similar to many including: Aquifex aeolicus TR:O67155 (EMBL; AE000720) hypothetical 48.6 KD protein (426 aa), fasta scores opt: 1099 z-score: 1189.0 E(): 0 46.1% identity in 408 aa overlap and Mycobacterium tuberculosis SW:Y03F_MYCTU (EMBL; Z73902) hypothetical 54.3 KD protein MTCY130.15C (509 aa), fasta scores opt: 825 z-score: 892.7 E(): 0 41.2% identity in 430 aa overlap. 3 4 6 7 [Reporter]SCO0156 (15K13)_PCR/7171 [Gene]SCO0156/6538 NC_003888 SCJ1.05c, doubtful CDS, len: 125 aa 3 4 6 6 [Reporter]SCO0485 (16G13)_PCR/7170 [Gene]SCO0485/6537 NC_003888 SCF34.04, possible tetR-family transcriptional regulator, len: 203 aa; shows weak similarity to SW:TCMR_STRGA (EMBL:M80674), TcmR, Streptomyces glaucescens tetracenomycin C transcriptional repressor (226 aa), fasta scores; opt: 201 z-score: 254.2 E(): 8.4e-07, 32.2% identity in 152 aa overlap and to many putative transcriptional regulators. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Contains probable helix-turn-helix motif at aa 38-59 (Score 1212, +3.31 SD) 3 4 6 5 [Reporter]SCO0211 (17C13)_PCR/7169 [Gene]SCO0211/6536 NC_003888 SCJ12.23, unknown, len: 238 aa. Weakly similar, in parts, to many putative two component sensor kinases e.g. E. coli SW:NARQ_ECOLI (EMBL; M94724) nitrate/nitrite sensor protein NarQ (EC 2.7.3.-) (566 aa), fasta scores opt: 424 z-score: 475.7 E(): 3.9e-19 39.5% identity in 238 aa overlap and Streptomyces coelicolor TR:Q9ZBY4 (EMBL; AL034355) putative two component sensor SCD78.15 (560 aa), fasta scores opt: 424 z-score: 475.7 E(): 3.9e-19 39.5% identity in 238 aa overlap 3 4 6 4 [Reporter]SCO0142 (18O9)_PCR/7168 [Gene]SCO0142/6535 NC_003888 SCJ33.06c, unknown, len: 601 aa. Similar to the C-terminus of Mycobacterium tuberculosis hypothetical proteins SW:YS74_MYCTU (EMBL; Z74024) (695 aa), fasta scores opt: 331 z-score: 375.0 E(): 1.6e-13 30.3% identity in 185 aa overlap and SW:YX05_MYCTU (EMBL; Z74024) (695 aa), fasta scores opt: 331 z-score: 375.0 E(): 1.6e-13 30.3% identity in 185 aa overlap. Contains 5x Pfam match to entry PF01436 NHL, NHL repeat and possible membrane spanning hydrophobic domains 3 4 6 3 [Reporter]SCO7464 (19K9)_PCR/7167 [Gene]SCO7464/6534 NC_003888 SCBAC14E8.04, cvnB13, hypothetical protein, len: 133 aa; similar to TR:Q9L138 (EMBL:AL158061) Streptomyces coelicolor hypothetical 14.a kDa protein SC6D11.17c, 135 aa; fasta scores: opt: 321 Z-score: 425.0 bits: 84.4 E(): 4.3e-16; 40.476% identity in 126 aa overlap 3 4 6 2 [Reporter]SCO7518 (20C9)_PCR/7166 [Gene]SCO7518/6533 NC_003888 SCBAC25F8.10c, possible tetR-family transcriptional regulatory protein, len: 185 aa; similar to TR:Q9F2S8 (EMBL:AL392176) Streptomyces coelicolor putative tetR-family transcriptional regulatory protein SCD65.04c, 185 aa; fasta scores: opt: 501 Z-score: 575.5 bits: 113.1 E(): 1.9e-24; 44.134% identity in 179 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 30..51 (+2.59 SD) 3 4 5 22 [Reporter]SCO5379 (1C5)_PCR/7164 [Gene]SCO5379/6532 NC_003888 2SC6G5.23, possible membrane protein, len: 194aa; similar to TR:O31780 (EMBL:Z99112) hypothetical protein from Bacillus subtilis (185 aa) fasta scores; opt: 399, z-score: 492.4, E(): 5.9e-20, 34.3% identity in 178 aa overlap. Contains possible membrane-spanning hydrophobic regions. Also contains Prosite match to PS00622 Bacterial regulatory proteins, luxR family signature but no similarity to any other regulators. 2 3 12 3 [Reporter]SCO6102 (19B18)_PCR/4780 [Gene]SCO6102/4359 NC_003888 SCBAC1A6.26c, probable nitrite/sulphite reductase, len: 565 aa; similar to SW:NIR_SYNP7 (EMBL:D12723) Synechococcus sp. ferredoxin--nitrite reductase (EC 1.7.7.1) NirA, 512 aa; fasta scores: opt: 935 z-score: 1113.1 E(): 0; 35.6% identity in 525 aa overlap. Contains Pfam match to entry PF01077 NIR_SIR, Nitrite and sulphite reductase 4Fe-4S domain 3 4 5 21 [Reporter]SCO5372 (2O1)_PCR/7163 [Gene]SCO5372/6531 NC_003888 2SC6G5.16, atpG, ATP synthase gamma chain (EC 3.6.1.34), len: 305aa; strongly similar to many eg. SW:P00837 (ATPG_ECOLI) ATP synthase gamma chain from Escherichia coli (287 aa) fasta scores; opt: 591, z-score: 655.5, E(): 4.9e-29, 37.4% identity in 289 aa overlap. Almost identical to SW:P50007 (ATPG_STRLI) ATP synthase gamma chain from Streptomyces lividans. Contains Pfam match to entry PF00231 ATP-synt, ATP synthase and Prosite match to PS00153 ATP synthase gamma subunit signature. 2 3 12 2 [Reporter]SCO7502 (20J14)_PCR/4779 [Gene]SCO7502/4358 NC_003888 SCBAC17A6.35c, possible LacI-family transcriptional regulator, len: 346 aa; similar to many e.g. SW:P46828 (CCPA_BACME) glucose-resistance amylase regulator CcpA from Bacillus megaterium (332 aa) fasta scores: opt: 528, Z-score: 565.6, 30.769% identity (31.902% ungapped) in 338 aa overlap and TR:Q9K433 (EMBL:AL359779) putative LacI-family transcriptional regulator from Streptomyces coelicolor (340 aa) fasta scores: opt: 993, Z-score: 1055.5, 49.422% identity (51.506% ungapped) in 346 aa overlap. Contains Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family; Pfam match to entry PF00356 lacI, Bacterial regulatory proteins, lacI family and Prosite match to PS00356 Bacterial regulatory proteins, lacI family signature. 3 4 5 20 [Reporter]SCO5880 (3K1)_PCR/7162 [Gene]SCO5880/6530 NC_003888 SC2E9.21, redY, unknown function, len: 106 2 3 11 22 [Reporter]SCO1253 (1J10)_PCR/4777 [Gene]SCO1253/4357 NC_003888 2SCG1.28, conserved hypothetical protein, len: 260 aa; similar to TR:Q9X7W9 (EMBL:AL049485) Streptomyces coelicolor hypothetical 28.1 kD protein SC6A5.23, 261 aa; fasta scores: opt: 646 z-score: 748.3 E(): 0; 44.0% identity in 250 aa overlap 2 3 11 21 [Reporter]SCO0613 (2F10)_PCR/4776 [Gene]SCO0613/4356 NC_003888 SCF55.37, arcA, arginine deiminase, len: 420 aa; similar to SW:ARCA_PSEAE (EMBL:X14694) Pseudomonas aeruginosa arginine deiminase (EC 3.5.3.6) ArcA, 417 aa; fasta scores: opt: 1807 z-score: 2137.4 E(): 0; 61.7% identity in 412 aa overlap 2 3 11 20 [Reporter]SCO4814 (3B10)_PCR/4775 [Gene]SCO4814/4355 NC_003888 SCD63A.25, purH, bifunctional purine biosynthesis protein, len: 523 aa; highly similar to TR:AAF05727 (EMBL:AF191543) Mycobacterium paratuberculosis phosphoribosylaminoimidazolecarboxamide/formyltransferase, PurH, 527 aa; fasta scores: opt: 2301 z-score: 2632.5 E(): 0; 69.1% identity in 518 aa overlap. Contains Pfam match to entry PF01808 AICARFT_IMPCHas, AICARFT/IMPCHase bienzyme 2 3 11 18 [Reporter]SCO2194 (5J6)_PCR/4773 [Gene]SCO2194/4354 NC_003888 SC5F7.07, lipA, probable lipoic acid synthetase, len: 317aa; similar to many egs. SW:LIPA_MYCTU probable lipoic acid synthetase from Mycobacterium tuberculosis (311 aa) fasta scores; opt: 1564, z-score: 1838.3, E(): 0, (72.9% identity in 314 aa overlap) and SW:LIPA_ECOLI lipoic acid synthetase from Escherichia coli (321 aa) fasta scores; opt: 814, z-score: 959.8, E(): 0, (41.9% identity in 313 aa overlap). 2 3 11 17 [Reporter]SCO2045 (6F6)_PCR/4772 [Gene]SCO2045/4353 NC_003888 SC4G6.14, unknown, len: 211aa; similar to TR:P95285 (EMBL:Z84498) hypothetical protein from Mycobacterium tuberculosis (214 aa) fasta scores; opt: 416, z-score: 500.9, E(): 1.4e-20, (39.8% identity in 206 aa overlap). 1 2 18 7 [Reporter]SCO2552 (16I3)_PCR/2396 [Gene]SCO2552/2179 NC_003888 SCC77.19c, sdrD, hypothetical protein, len: 249 aa. Previously sequenced from Streptomyces coelicolor A3(2) TR:Q02798(EMBL:X68792) hypothetical protein in pth270 promoter (fragment) (> 111 aa). Two possible translated products are encoded by this CDS, the alternative translational start site being at residue 5 (EMBL:AF170561). This protein is possibly involved in differentiation. Similar to proteins of undefined function from Mycobacterium tuberculosis TR:O05826(EMBL:Z95208) (262 aa), fasta scores opt: 624 z-score: 716.8 E(): 1.5e-32 45.1% identity in 253 aa overlap and Bacillus subtilis SW:YQEU_BACSU(EMBL:D84432) (256 aa), fasta scores opt: 329 z-score: 382.4 E(): 6.6e-14 27.2% identity in 250 aa overlap 2 3 11 16 [Reporter]SCO7276 (7B6)_PCR/4771 [Gene]SCO7276/4352 NC_003888 SC5H1.16, hypothetical protein, len: 194 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Start codon position suggested by GC frame plot and possible RBS. Contains region similar to adjacent CDS, SC5H1.17, hypothetical protein (138 aa) (34.6% identity in 107 aa overlap) 1 2 18 6 [Reporter]SCO0880 (17E3)_PCR/2395 [Gene]SCO0880/2178 NC_003888 SCM1.13, possible membrane protein, len: 138 aa. Contains a possible membrane spanning hydrophobic domain and an N-terminal signal sequence. 2 3 11 15 [Reporter]SCO5571 (8N2)_PCR/4770 [Gene]SCO5571/4351 NC_003888 SC7A1.15, rpmF, 50S ribosomal protein L32, len: 57 aa; similar to many eg. SW:RL32_BACST 50S PrmF, ribosomal protein L32 from Bacillus stearothermophilus (56 aa) fasta scores; opt: 180, z-score: 341.1, E(): 1.1e-11, (48.1% identity in 54 aa overlap). 2 1 1 4 [Reporter]SCO0559 (18J8)_PCR/507 [Gene]SCO0559/459 NC_003888 SCF73.06c, unknown, len: 333 aa 1 2 18 4 [Reporter]SCP1.138 (18M23)_PCR/2393 [Gene]SCP1.138/2177 NC_003888 SCP1.138, parA1, possible plasmid partitioning protein, len: 420aa; similar to many eg. TR:Q9RFM1 (EMBL:AF187159) ParA partitioning protein from Streptomyces coelicolor (357 aa) fasta scores; opt: 185, z-score: 214.7, E(): 0.00017, 29.0% identity in 314 aa overlap. Contains Pfam match to entry PF00991 ParA, ParA family ATPase. This CDS is directly duplicated at 221485..222648 (ParA2, SCP1.221) fasta scores; opt: 980, z-score: 1089.9, E(): 0, 55.2% identity in 424 aa overlap. 2 3 11 14 [Reporter]SCO2649 (9J2)_PCR/4769 [Gene]SCO2649/4350 NC_003888 SC8E4A.19c, probable 4-alpha-glucanotransferase, len: 711 aa; similar to SW:MALQ_MYCTU (EMBL:AL022021) Mycobacterium tuberculosis 4-alpha-glucanotransferase (EC 2.4.1.25) MalQ, 724 aa; fasta scores: opt: 2038 z-score: 2288.0 E(): 0; 47.9% identity in 727 aa overlap and to SW:MALQ_ECOLI (EMBL:M32793) Escherichia coli 4-alpha-glucanotransferase (EC 2.4.1.25) MalQ, 694 aa; fasta scores: opt: 979 z-score: 1098.2 E(): 0; 33.3% identity in 654 aa overlap 2 1 1 3 [Reporter]SCP1.320c (19F8)_PCR/506 [Gene]SCP1.320c/458 NC_003888 SCP1.320c, possible conserved integral membrane protein, len: 312aa; similar to many from Streptomyces coelicolor eg. TR:O69922 (EMBL:AL023861) putative integral membrane protein (367 aa) fasta scores; opt: 613, z-score: 713.6, E(): 2.8e-32, 44.6% identity in 222 aa overlap. Contains multiple possible membrane-spanning hydrophobic regions. 1 2 18 3 [Reporter]SCO1858 (19I23)_PCR/2392 [Gene]SCO1858/2176 NC_003888 SCI39.05, conserved hypothetical protein, len: 305 aa; similar to TR:O87690 (EMBL:AJ000758) Bacillus megaterium Cbi protein CbiX, 306 aa; fasta scores: opt: 791 Z-score: 870.2 bits: 169.1 E(): 7e-41; 39.000% identity in 300 aa overlap. Contains Pfam match to entry PF01903 CbiX, CbiX 1 1 23 22 [Reporter]SCO4923 (1I24)_PCR/504 [Gene]SCO4923/457 NC_003888 SCK13.15c, conserved hypothetical protein, len: 206 aa; similar to TR:P74331 (EMBL:D90914) Synechocystis sp. hypothetical protein Maf, 195 aa; fasta scores: opt: 565 z-score: 677.1 E(): 3.5e-30; 49.2% identity in 193 aa overlap 1 2 18 2 [Reporter]SCO7474 (20A23)_PCR/2391 [Gene]SCO7474/2175 NC_003888 SCBAC17A6.07, possible phenylacetic acid degradation protein PaaD, len: 170 aa: similar to many e.g. SW:P76080 (PAAD_ECOLI) phenylacetic acid degradation protein PaaD from Escherichia coli (167 aa) fasta scores; opt: 474, Z-score: 518.6, 45.395% identity (47.586% ungapped) in 152 aa overlap. Contains Pfam match to entry PF01883 DUF59, Domain of unknown function DUF59. 1 1 23 21 [Reporter]SCO3073 (2E24)_PCR/503 [Gene]SCO3073/456 NC_003888 SCE25.14c, hutU, urocanate hydratase, len: 572 aa; highly similar to SW:HUTU_BACSU (EMBL:D31856) Bacillus subtilis urocanate hydratase (EC 4.2.1.49) (urocanase) (imidazolonepropionate hydrolase) HutU, 552 aa; fasta scores: opt: 2440 z-score: 2699.9 E():0; 66.1% identity in 548 aa overlap. Contains Pfam match to entry PF01175 Urocanase, Urocanase and match to Prosite entry PS01233 Urocanase active site 1 2 17 22 [Reporter]SCO1119 (1A19)_PCR/2389 [Gene]SCO1119/2174 NC_003888 2SCG38.12, probable asnC-family transcriptional regulatory protein, len: 158 aa; similar to SW:PUTR_RHOCA (EMBL:X78346) Rhodobacter capsulatus proline dehydrogenase transcriptional activator PutR, 154 aa; fasta scores: opt: 245 z-score: 306.6 E(): 1.2e-09; 34.3% identity in 143 aa overlap. Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family and possible helix-turn-helix motif at residues 20..41 (+2.62 SD) 1 1 23 20 [Reporter]SCO6327 (3A24)_PCR/502 [Gene]SCO6327/455 NC_003888 SC10H5.03c, probable integral membrane protein, len: 154 aa. Contains possible hydrophobic membrane spanning regions 1 2 17 21 [Reporter]SCO5803 (2M15)_PCR/2388 [Gene]SCO5803/2173 NC_003888 SC4H2.24c, lexA, probable SOS regulatory protein LexA, len: 234 aa; similar to many LEXA_BACSU P31080 SOS regulatory protein LexA/DinR (205 aa), fasta scores; opt: 613 z-score: 633.7 E(): 4.5e-28, 46.4% identity in 209 aa overlap (active site residues are conserved) 1 1 23 19 [Reporter]SCO6220 (4M20)_PCR/501 [Gene]SCO6220/454 NC_003888 SC2H4.02, unknown, len: 2183; very limited similarity to proteins of the RHS family e.g. WAPA_BACSU wall-associated protein precursor (2334 aa), fasta scores; opt: 141 z-score: 296.8 E(): 2.7e-09, 23.4% identity in 1237 aa overlap, and RHSA_ECOLI RHSA protein precursor (1377 aa), fasta scores; opt: 135 z-score: 222.4 E(): 3.8e-05, 23.0% identity in 1016 aa overlap. Contains possible membrane anchor around aa 1900. Alternative start at aa 27 would give N-terminal signal sequence 1 2 17 20 [Reporter]SCO6812 (3I15)_PCR/2387 [Gene]SCO6812/2172 NC_003888 SC1A2.21, possible ArsR-family transcriptional regulator, len: 119 aa. Highly similar to several including: Streptomyces coelicolor TR:Q9X8X8 (EMBL:AL078610) putative transcriptional regulator, SCH35.28C (122 aa), fasta scores opt: 485 z-score: 630.1 E(): 1.1e-27 69.7% identity in 99 aa overlap and Mycobacterium tuberculosis TR:P71941 (EMBL:Z80225) putative transcriptional regulator (126 aa), fasta scores opt:378 z-score: 494.4 E(): 3.9e-20 59.8% identity in 97 aa overlap. Also similar to the adjoining CDS SC1A2.22 (113 aa), fasta scores opt: 292 z-score: 353.0 E(): 3.1e-14 51.1% identity in 94 aa overlap. Contains a Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family. 1 1 23 18 [Reporter]SCO7039 (5I20)_PCR/500 [Gene]SCO7039/453 NC_003888 SC4G1.05c, possible ABC transport system ATP-binding protein, len: 130 aa; similar to SW:DPPF_ECOLI (EMBL:L08399) Escherichia coli dipeptide transport ATP-binding protein DppF, 334 aa; fasta scores: opt: 177 z-score: 195.7 E(): 0.002; 42.9% identity in 70 aa overlap 1 2 17 18 [Reporter]SCO2383 (5A15)_PCR/2385 [Gene]SCO2383/2171 NC_003888 SC4A7.11, possible secreted protein, len: 1545 aa; similar in its central part to SW:STFR_ECOLI (EMBL:AE000234) Escherichia coli side tail fiber protein homolog from labmboid prophage RAC StfR or B1372, 1120 aa; fasta scores: opt: 494 Z-score: 414.4 E(): 1.9e-15; 31.461% identity in 445 aa overlap. Contains high percentage of aa residues A and G. Contains Pfam match to entry PF00652 Ricin_B_lectin, Similarity to lectin domain of ricin beta-chain and possible N-terminal region signal peptide sequence. Also contains possible colied-coil regions at approx. residues 655..761, 789..846 and 1098..1163 1 1 23 17 [Reporter]SCO7571 (6E20)_PCR/499 [Gene]SCO7571/452 NC_003888 SC5F1.25, conserved hypothetical protein, len: 137 aa; similar to SW:Y854_PYRHO (EMBL:AP000003) Pyrococcus horikoshii hypothetical protein PH0854, 137 aa; fasta scores: opt: 326 z-score: 392.0 E(): 2.7e-14; 44.3% identity in 122 aa overlap. Contains Pfam match to entry PF01042 UPF0076, Domain of unknown function UPF0076 1 2 17 17 [Reporter]SCO6530 (6M11)_PCR/2384 [Gene]SCO6530/2170 NC_003888 SC5C7.15, unknown, len: 610 aa; contains Gln- and Lys-rich N-terminus 1 1 23 16 [Reporter]SCO7408 (7A20)_PCR/498 [Gene]SCO7408/451 NC_003888 SC6D11.04c, probable solute-binding lipoprotein, len: 451 aa. Similar to several other transport proteins including: Agrobacterium radiobacter SW:LACE_AGRRD(EMBL:X66596) lactose-binding protein precursor, LacE (422 aa), fasta scores opt: 347 z-score: 393.9 E(): 1.6e-14 24.0% identity in 425 aa overlap and Streptomyces coelicolor TR:CAB61177(EMBL:AL132973) probable solute-binding lipoprotein, SCF91.20 (443 aa), fasta scores opt: 550 z-score: 621.9 E(): 3.2e-27 26.2% identity in 455 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains a Prosite hit to PS01037 Bacterial extracellular solute-binding proteins, family 1 signature and a Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein. 1 1 23 15 [Reporter]SCO2010 (8M16)_PCR/497 [Gene]SCO2010/450 NC_003888 SC7H2.24, probable branched chain amino acid transport permease, len: 310aa; region from 200-608aa similar to many eg. SW:LIVM_SALTY high affinity branched chain amino acid transport permease from Salmonella typhimurium (425 aa) fasta scores; opt: 633, z-score: 702.7, E(): 8.1e-32, (36.8% identity in 400 aa overlap). 3 4 5 19 [Reporter]SCO6160 (4G1)_PCR/7161 [Gene]SCO6160/6529 NC_003888 SC1A9.24c, SecDF, protein-export membrane protein, len: 795aa; similar to many eg. TR:G3220156 (EMBL:AF024506) SecDF protein from Bacillus subtilis (737 aa) fasta scores; opt:802, z-score:1099.4, E():0, (30.7% identity in 740 aa overlap). 3 4 5 18 [Reporter]SCO7095 (5C1)_PCR/7160 [Gene]SCO7095/6528 NC_003888 SC3A4.21c, possible hydrolase. len: 318 aa. Similar in parts to several e.g. Moraxella sp. SW:DEH1_MORSP(EMBL:D90422) haloacetate dehalogenase H-1 (EC 3.8.1.3) (294 aa), fasta scores opt: 245 z-score: 286.4 E(): 1.7e-08 37.4% identity in 131 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 3 4 5 17 [Reporter]SCO7054 (5O21)_PCR/7159 [Gene]SCO7054/6527 NC_003888 SC4G1.20, conserved hypothetical protein, len: 195 aa; similar to TR:Q9X399 (EMBL:AF06544) Vibrio cholerae hypothetical 20.4 kD protein AphA, 179 aa; fasta scores: opt: 313 z-score: 386.3 E(): 4.8e-14; 32.4% identity in 185 aa overlap 3 4 5 16 [Reporter]SCO7313 (6K21)_PCR/7158 [Gene]SCO7313/6526 NC_003888 SC5F8.23, unknown, len: 148 aa. Weakly similar to several putative regulatory proteins e.g. Streptomyces coelicolor A3(2) TR:Q9RIS7(EMBL:AJ249581) putative regulator of sig15 (170 aa), fasta scores opt: 144 z-score: 184.4 E(): 0.0084 36.1% identity in 133 aa overlap and Streptomyces coelicolor TR:Q9RD41(EMBL:AL133422) SCM1.01C (147 aa), fasta scores opt: 225 z-score: 281.4 E(): 3.3e-08 36.6% identity in 131 aa overlap. 3 4 5 15 [Reporter]SCO7423 (7G21)_PCR/7157 [Gene]SCO7423/6525 NC_003888 SC6D11.19c, unknown, len: 236 aa. Weakly similar to a protein of undefined function from Rhodococcus rhodochrous plasmid pKA22 TR:Q9XBN0(EMBL:AF165152) hypothetical 25.4 KD protein (242 aa), fasta scores opt: 336 z-score: 419.4 E(): 6.1e-16 33.0% identity in 209 aa overlap. 3 4 5 14 [Reporter]SCO6887 (8C21)_PCR/7156 [Gene]SCO6887/6524 NC_003888 SC7F9.39, hypothetical protein, len: 161 aa. Highly similar to Streptomyces coelicolor TR:Q9X8A8 (EMBL:AL049645) hypothetical 17.2 KD protein, SCE2.13 (158 aa), fasta scores opt: 252 z-score: 254.1 E(): 9.7e-07 40.0% identity in 145 aa overlap. 3 4 5 13 [Reporter]SCO6136 (9O17)_PCR/7155 [Gene]SCO6136/6523 NC_003888 SC9B2.23c, possible transmembrane protein, unknown function, len: 339aa; similar to TR:O53316 (EMBL:AL021646) putative transmembrane protein from Mycobacterium tuberculosis (319 aa) fasta scores; opt: 247, z-score: 240.4, E(): 4.2e-06, (28.2% identity in 326 aa overlap). Contains 3 possible membrane spanning regions. 3 4 5 12 [Reporter]SCO1951 (10K17)_PCR/7154 [Gene]SCO1951/6522 NC_003888 SCC54.11c, unknown, len: 363aa; similar to many hypothetical proteins eg. SW:YR40_MYCTU from Mycobacterium tuberculosis (342 aa) fasta scores; opt: 1047, z-score: 1131.4, E(): 0, (54.5% identity in 308 aa overlap) 2 3 11 13 [Reporter]SCO6487 (10F2)_PCR/4768 [Gene]SCO6487/4349 NC_003888 SC9C7.23, possible aminoacylase, len: 443aa; similar to many aminoacylases and hypothetical proteins egs. TR:O06234 (EMBL:Z95388) hypothetical protein from Mycobacterium tuberculosis (448 aa) fasta scores; opt: 1278, z-score: 1467.2, E(): 0, (47.2% identity in 449 aa overlap) and SW:ACY1_PIG aminoacylase I from Sus scrofa (pig) (406 aa) fasta scores; opt: 294, z-score: 340.6, E(): 1.1e-11, (27.3% identity in 421 aa overlap). Contains PS00758 ArgE / dapE / ACY1 / CPG2 / yscS family signature 1. 3 4 5 11 [Reporter]SCO4360 (11G17)_PCR/7153 [Gene]SCO4360/6521 NC_003888 SCD19.15, probable ABC transport system integral membrane protein, len: 280 aa; similar to SW:DRRB_STRPE (EMBL:M73758) Streptomyces peucetius daunorubicin resistance transmembrane protein DrrB, 283 aa; fasta scores: opt: 413 z-score: 474.0 E(): 6.8e-19; 31.6% identity in 244 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 11 12 [Reporter]SCO2711 (11B2)_PCR/4767 [Gene]SCO2711/4348 NC_003888 SCC61A.32, possible glycosyl transferase, len: 400 aa; weakly similar to others proposed to be involved in LPS biosynthesis e.g. TR:AAF23993 (EMBL:AF035937) from Pseudomonas aeruginosa (383 aa) fasta scores; opt: 381, z-score: 364.0, E(): 8.2e-13, 28.6% identity in 392 aa overlap. Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1. 3 4 5 10 [Reporter]SCO4276 (12C17)_PCR/7152 [Gene]SCO4276/6520 NC_003888 SCD95A.09, probable respose regulatory protein, len: 223 aa; highly similar to TR:CAA74720 (EMBL:Y14336) Streptomyces reticuli respose regulatory protein SenR, 217 aa; fasta scores: opt: 1066 z-score: 1252.4 E(): 0; 80.4% identity in 219 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Contains also possible helix-turn-helix motif at residues 177..198 (+4.22 SD) 2 3 11 11 [Reporter]SCO4424 (11N22)_PCR/4766 [Gene]SCO4424/4347 NC_003888 SCD6.02, possible secreted protein, len: 176 aa; almost identical to TR:Q53910 (EMBL:D45382) Streptomyces coelicolor ORFB, 175 aa. Contains possible N-terminal region signal peptide sequence 2 3 11 10 [Reporter]SCO3535 (12J22)_PCR/4765 [Gene]SCO3535/4346 NC_003888 SCE2.16, unknown, len: 706aa; 2 3 11 9 [Reporter]SCO5625 (13F22)_PCR/4764 [Gene]SCO5625/4345 NC_003888 SC2E1.42, tsf, elongation factor Ts, len: 278. Almost identical to EFTS_STRCO elongation factor TS (EF-TS) (276 aa), fasta scores; opt: 1695 z-score: 2425.0 E(): 0, 97.8% identity in 278 aa overlap and highly similar to many others e.g. EFTS_ECOLI (282 aa), fasta scores; opt: 321 z-score: 681.2 E(): 1e-30, 38.4% identity in 276 aa overlap. Contains PS01126 Elongation factor Ts signature 1 2 3 11 8 [Reporter]SCO0499 (14B22)_PCR/4763 [Gene]SCO0499/4344 NC_003888 SCF34.18, possible formyltransferase, len: 315 aa; similar to TR:Q50378 (EMBL:U10425), FxbA, Mycobacterium smegmatis putative formyltransferase required for exochelin biosynthesis (360 aa), fasta scores; opt: 942 z-score: 1102.4 E(): 0, 49.5% identity in 309 aa overlap. Also similar to many methionyl-tRNA formyltransferases e.g. SW:FMT_PSEAE (EMBL:AF073952), Fmt, Pseudomonas aeruginosa methionyl-tRNA formyltransferase (314 aa) (30.3% identity in 307 aa overlap). Contains Pfam match to entry PF00551 formyl_transf, Formyl transferase 2 3 11 7 [Reporter]SCO3588 (15N18)_PCR/4762 [Gene]SCO3588/4343 NC_003888 SCH66.09, unknown, len: 387 aa; similar to TR:O32505 (EMBL:AB003475) hypothetical protein from Deinococcus radiodurans (383 aa) fasta scores; opt: 157, z-score: 184.7, E(): 0.0058, (27.1% identity in 225 aa overlap). 1 2 17 16 [Reporter]SCO5666 (7I11)_PCR/2383 [Gene]SCO5666/2169 NC_003888 SC8B7.12c, probable aldehyde dehydrogenase, partial CDS, len: >315 aa; similar to many e.g. DHAB_ECOLI betaine aldehyde dehydrogenase (489 aa), fasta scores; opt: 861 z-s core: 1174.4 E(): 0, 44.2% identity in 301 aa overlap. Also similar to upstream ORF SC6A9.10c (E(): 0, 45.6% identity in 281 aa overlap). Contains PS00687 Aldehyde dehydrogenases glutamic acid active site, PS00070 Aldehyde dehydrogenases cysteine active site and Pfam match to entry PF00171 alde dh, Aldehyde dehydrogenases, score 153.00, E-value 5.3e-42. Overlaps and extends SC6A9.01c,SC6A9.01c, probable aldehyde dehydrogenase, partial CDS len: 280 aa; highly similar to many e.g. FEAB_ECOLI phenylacetaldehyde dehydrogenase (499 aa), fasta scores; opt: 718 z-score: 924.3 E(): 0, 43.6% identity in 264 aa overlap. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site 2 3 11 6 [Reporter]SCO5046 (16J18)_PCR/4761 [Gene]SCO5046/4342 NC_003888 SCK7.19, wblI, len: 125 aa; identical to previously sequenced TR:Q9X953 (EMBL:AJ239088) Streptomyces coelicolor hypothetical 13.9 kDa protein WblI, 125 aa and to TR:O88103 (EMBL:AJ010601) Streptomyces coelicolor WhiD protein, 112 aa; fasta scores: opt: 297 z-score: 395.0 E(): 1.6e-14; 46.7% identity in 105 aa overlap 1 2 17 15 [Reporter]SCO5301 (8E11)_PCR/2382 [Gene]SCO5301/2168 NC_003888 SC6G9.32, possible secreted penicillin-binding protein, len: 485 aa; similar to many e.g. to the C-terminal end of SW:PBPC_BACSU (EMBL:D38161), PbpC, Bacillus subtilis penicillin-binding protein 3 (668 aa), fasta scores; opt: 422 z-score: 451.6 E(): 8e-18, 27.6% identity in 384 aa overlap. Similar to putative penicillin-binding proteins from mycobacteria e.g. TR:P71586 (EMBL:Z80233) Mycobacterium tuberculosis PpbA homologue (491 aa) (42.8% identity in 495 aa overlap). Contains a hydrophobic, possible membrane anchor at the N-terminus. Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and PS00017 ATP/GTP-binding site motif A (P-loop) 2 3 11 5 [Reporter]SCO0126 (17F18)_PCR/4760 [Gene]SCO0126/4341 NC_003888 SCJ21.07, probable multi-domain beta keto-acyl synthase, len: 2082 aa; similar to proteins involved in fatty acid or polyketide biosynthesis e.g. TR:O30776 (EMBL:) heterocyst glycolipid synthase HglE (1053 aa), fasta scores; opt: 2382 z-score: 1897.1 E(): 0, 41.6% identity in 1013 aa overlap, and TR:O54142 (EMBL:AL021530) S. coelicolor polyketide synthase RedX (982 aa), fasta scores; opt: 910 z-score: 728.5 E(): 0, 37.4% identity in 494 aa overlap. Contains 2x Pfam matches to entry PF00109 ketoacyl-synt, Beta-ketoacyl synthase, and Pfam match to entry PF00550 pp-binding, Phosphopantetheine attachment site 1 1 23 14 [Reporter]SCO6120 (9I16)_PCR/496 [Gene]SCO6120/449 NC_003888 SC9B2.07, unknown, len: 162 aa; similar to TR:E1319700 (EMBL:AL031541) unknown ORF in IS117 from Streptomyces coelicolor (175 aa) fasta scores; opt: 267, z-score: 323.9, E(): 9.4e-11, (32.7% identity in 162 aa overlap). Also similar to SW:YM2_STRCO hypothetical protein in the mini-circle, a transposable element of Streptomyces coelicolor A3(2) (122 aa) fasta scores; opt: 217, z-score: 268.1, E(): 1.2e-07, (34.2% identity in 120 aa overlap). 1 2 17 14 [Reporter]SCO5510 (9A11)_PCR/2381 [Gene]SCO5510/2167 NC_003888 SC8D9.22, probable dehydrogenase, len: 313aa; similar to many both eukaryotic and prokaryotic egs. SW:FDH_SOLTU NAD-dependent formate dehydrogenase from the mitochondria of Solanum tuberosum (potato) (379 aa) fasta scores; opt: 372, z-score: 413.2, E(): 1e-15, (29.9% identity in 278 aa overlap) and SW:SERA_BACSU D-3-phosphoglycerate dehydrogenase from Bacillus subtilis (525 aa) fasta scores; opt: 348, z-score: 384.9, E(): 3.9e-14, (32.4% identity in 256 aa overlap). Contains PS00671 D-isomer specific 2-hydroxyacid dehydrogenases signature 3 and Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases, score 147.40, E-value 5.1e-60. 2 3 11 4 [Reporter]SCO1453 (18B18)_PCR/4759 [Gene]SCO1453/4340 NC_003888 SCL6.10, conserved hypothetical protein, len: 310 aa; similar to various hypothetical proteins, e.g. SW:Y953_MYCTU (EMBL:Z79700) Mycobacterium tuberculosis hypothetical 30.9 kD protein RV0953c, 282 aa; fasta scores: opt: 405 z-score: 467.1 E(): 1.4e-18; 33.6% identity in 265 aa overlap 1 1 23 13 [Reporter]SCO2442 (10E16)_PCR/495 [Gene]SCO2442/448 NC_003888 SCC24.13c, probable gntR-family transcriptional regulator, len: 235 aa; similar to SW:UXUR_ECOLI (EMBL:U14003) Escherichia coli, Uxu operon transcriptional regulator UxuR, 257 aa; fasta scores: opt: 338 z-score: 385.5 E(): 4.9e-14; 32.6% identity in 236 aa overlap and to TR:CAB6127 (EMBL:AL132991) Streptomyces coelicolor putative transcriptional regulator SCF55.06, 253 aa; fasta scores: opt: 380 z-score: 432.1 E(): 1.2e-16; 33.1% identity in 236 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and match ot Prosite entry S00043 Bacterial regulatory proteins, gntR family signature and possible helix-turn-helix motif at residues 37..58 (+3.83 SD) 1 2 17 12 [Reporter]SCO4385 (11I7)_PCR/2379 [Gene]SCO4385/2166 NC_003888 SCD10.17, probable tetR-family transcriptional regulator, len: 202 aa; similar to TR:O33453 (EMBL:U24215) Pseudomonas putida regulatory protein for cym and cmt operons; cym repressor CymR, 203 aa; fasta scores: opt: 245 z-score: 309.4 E(): 9.3e-10; 31.4% identity in 191 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Contains also a possible helix-turn-helix motif at residues 39..60 (+3.02 SD) 1 1 23 12 [Reporter]SCO4114 (11A16)_PCR/494 [Gene]SCO4114/447 NC_003888 SCD17A.06c, sporulation associated protein, len: 473 aa; identical to previously sequenced TR:Q53958 (EMBL:M80614) Streptomyces coelicolor sporulation associated protein, 473 aa and similar to TR:Q9S2P4 (EMBL:AL096872) Streptomyces coelicolor putative regulator SC5F7.09c, 487 aa; fasta scores: opt: 728 z-score: 658.8 E(): 4.7e-31; 39.7% identity in 489 aa overlap. Contains a possible cleavable N-terminal region signal peptide sequence and a TTA leucine codon, possible target for bldA regulation 1 2 17 11 [Reporter]SCO4459 (12E7)_PCR/2378 [Gene]SCO4459/2165 NC_003888 SCD65.02c, possible membrane protein, len: 134 aa. Contains possible hydrophobic membrane spanning region. High alanine and glycine amino acid residues content 1 1 23 11 [Reporter]SCO4268 (12M12)_PCR/493 [Gene]SCO4268/446 NC_003888 SCD95A.01, unknown (fragment), len: >25 aa,SCD86A.05, hypothetical protein , len: 262 aa; similar to SW:Y516_SYNY3 (EMBL:D64004) Synechocystis sp. hypothetical 17.9 kDa protein SLR0516, 166 aa; fasta scores: opt: 177 z-score: 209.9 E(): 0.00032; 34.5% identity in 142 aa overlap. Contains 3x Pfam matches to entry PF00805 Pentapeptide, Pentapeptide repeats (8 copies) 1 2 17 10 [Reporter]SCO3078 (13A7)_PCR/2377 [Gene]SCO3078/2164 NC_003888 SCE25.19, possible secreted protein, len: 340 aa; similar to TR:O53423 (EMBL:AL021897) Mycobacterium tuberculosis hypothetical 32.9 kD protein MTV017.28c, 314 aa; fasta scotes: opt: 519 z-score: 544.7 E(): 7e-23; 38.3% identity in 332 aa overlap. Contains possible N-terminal region signal peptide sequence 1 1 23 10 [Reporter]SCO3451 (13I12)_PCR/492 [Gene]SCO3451/445 NC_003888 SCE46.08, unknown, len: 103 aa 1 2 17 9 [Reporter]SCO3930 (14M3)_PCR/2376 [Gene]SCO3930/2163 NC_003888 SCQ11.13, hypothetical protein, len: 188 aa; weakly similar to TR:Q53897 (EMBL:X60316) AbaA ORFA, in S. coelicolor pleiotropic regulatory locus for antibiotic production of actinorhodin (192 aa), fasta scores; opt: 146 z-score: 190.3 E(): 0.0029, 37.0% identity in 154 aa overlap. It is not known whether ORFA is involved in antibiotic production. Also weakly similar to SCE9.30 (EMBL:AL049841) S.coelicolor possible regulator containing TTA (leucine) codon near the N-terminus (154 aa) (31.3% identity in 131 aa overlap). Contains TTA (leucine) codon, possible target for bldA regulation 1 1 23 8 [Reporter]SCO0416 (15A12)_PCR/490 [Gene]SCO0416/444 NC_003888 SCF51.15c, probable integral membrane protein, len: 201 aa; similarity to SW:CMLR_STRLI (EMBL:X59968) Streptomyces lividans chloramphenicol resistance protein, 392aa; fasta scores: opt: 123 z-score: 149.4 E(): 0.6; 30.5% identity in 177 aa overlap 1 2 17 8 [Reporter]SCO0716 (15I3)_PCR/2375 [Gene]SCO0716/2162 NC_003888 SCF42.26, possible glycosyl hydrolase (putative secreted protein), len: 400 aa. Similar to several including: Arthrobacter sp. (strain YCWD3) SW:E13B_ARTSP(EMBL:D23668) glucan endo-1,3-beta-glucosidase precursor (EC 3.2.1.39) (548 aa), fasta scores opt: 425 z-score: 452.8 E(): 7.8e-18 34.5% identity in 414 aa overlap and Streptomyces matensis TR:Q9Z4I2(EMBL:AB019428) laminaripentaose-producing beta-1,3-guluase (lphase) precursor (401 aa), fasta scores opt: 477 z-score: 509.6 E(): 5.4e-21 38.7% identity in 413 aa overlap. Contains a possible N-terminal signal sequence 1 1 23 7 [Reporter]SCO0162 (16M8)_PCR/489 [Gene]SCO0162/443 NC_003888 SCJ1.11, unknown, similar to several including: Mycobacterium tuberculosis TR:O53476(EMBL; AL021899) hypothetical 36.6 KD protein (331 aa), fasta scores opt: 394 z-score: 468.4 E(): 1e-18 32.2% identity in 332 aa overlap and Mycobacterium tuberculosis TR:O05800 (EMBL; Z95150) hypothetical 38.5 KD protein (344 aa), fasta scores opt: 345 z-score: 410.5 E(): 1.7e-15 30.2% identity in 324 aa overlap. 1 2 17 7 [Reporter]SCO0374 (16E3)_PCR/2374 [Gene]SCO0374/2161 NC_003888 SCF41.33, putative ABC transport protein, len: 589 aa. Similar to many e.g. Streptomyces coelicolor TR:Q9ZNB0 (EMBL; AB019513) ABC transporter (584 aa), fasta scores opt: 597 z-score: 649.7 E(): 7.7e-29 30.9% identity in 572 aa overlap. Contains a Pfam match to entry PF00005 ABC_tran, ABC transporter and a PS00017 ATP/GTP-binding site motif A (P-loop). Contains membrane-spanning hydrophobic domains. 1 1 23 6 [Reporter]SCO0837 (17I8)_PCR/488 [Gene]SCO0837/442 NC_003888 SCF43A.27c, unknown, len: 88 aa; unknown function, improbable Pro/Gln-rich CDS suggested by weak GC frameplot, positional base preference and amino acid composition scores 1 2 17 6 [Reporter]SCO0642 (17A3)_PCR/2373 [Gene]SCO0642/2160 NC_003888 SCF91.02c, unknown, len: 581 aa; similar to TR:Q983G4 (EMBL:AP003013) from Mesorhizobium loti (559 aa) fasta scores: opt: 1692, Z-score: 1945.6, 46.277% identity (47.283% ungapped) in 564 aa overlap. 1 1 23 4 [Reporter]SCP1.93 (19A8)_PCR/486 [Gene]SCP1.93/441 NC_003888 SCP1.93, doubtful CDS, unknown, len: 142aa; 1 1 23 3 [Reporter]SCO1667 (20M4)_PCR/485 [Gene]SCO1667/440 NC_003888 SCI52.09, possible ion transport protein, len: 334 aa; similar to SW:CORA_ECOLI (EMBL:L11042) Escherichia coli magnesium and cobalt transport protein CorA, 316 aa; fasta scores: opt: 281 Z-score: 338.8 bits: 71.0 E(): 2.7e-11; 25.418% identity in 299 aa overlap and to TR:Q9FBK1 (EMBL:AL390975) Streptomyces coelicolor putative metal-transport protein SCP8.20c, 371 aa; fasta scores: opt: 1166 Z-score: 1386.9 bits: 265.1 E(): 1.1e-69; 53.801% identity in 342 aa overlap. Contains Pfam match to entry PF01544 CorA, CorA-like Mg2+ transporter protein and possible hydrophobic membranes spanning regions in the C-terminal domain 3 4 5 9 [Reporter]SCO3646 (13O13)_PCR/7151 [Gene]SCO3646/6519 NC_003888 SCH10.24c, probable oxidoreductase, len: 271 aa; weakly similar to tryptophan oxygenases e.g. TR:O77457 (EMBL:L76433) Anopheles gambiae tryptophan oxygenase (392 aa), fasta scores; opt: 184 z-score: 222.0 E(): 4.7e-05, 24.9% identity in 341 aa overlap 3 4 5 8 [Reporter]SCO1761 (14K13)_PCR/7150 [Gene]SCO1761/6518 NC_003888 SCI51.01c, possible cyclohexadienyl dehydrogenase, partial CDS, len: >212 aa; similar to several cyclohexadienyl dehydrogenases e.g. SW:TYRC_ZYMMO (EMBL:M75891), TyrC, Zymomonas mobilis cyclohexadienyl dehydrogenase (293 aa), fasta scores; opt: 299 z-score: 344.0 E(): 7.8e-12, 31.4% identity in 220 aa overlap. Also similar to other hypothetical oxidoreductases e.g. CZA382.29 (EMBL:AL078635) Streptomyces coelicolor putative oxidoreductase (367 aa) (55.8% identity in 208 aa overlap),2SCI34.14c, possible oxidoreductase (fragment) (putative secreted protein), len: >182 aa; similar to TR:Q9Z4W3 (EMBL:AL035654) Streptomyces coelicolor putative oxidoreductase SCE8.14c, 284 aa; fasta scores: opt: 301 z-score: 343.0 E(): 1.2e-11; 50.5% identity in 107 aa overlap. Contains possible N-terminal region signal peptide sequence 3 4 5 7 [Reporter]SCO0250 (15G13)_PCR/7149 [Gene]SCO0250/6517 NC_003888 SCJ9A.29, possible transcriptional regulator, len: 221aa; similar to many tetR-family transcriptional regulators eg. SW:TER1_ECOLI TetR from Tn1721 of Escherichia coli (216 aa) fasta scores; opt: 429, z-score: 484.8, E(): 1.2e-19, (40.7% identity in 209 aa overlap). Contains possible helix-turn-helix motif (+4.13 SD) 37-58aa. 3 4 5 6 [Reporter]SCO0701 (16C13)_PCR/7148 [Gene]SCO0701/6516 NC_003888 SCF42.11c, unknown, len: 147 aa. Identical to the previously published Streptomyces coelicolor TR:Q53898(EMBL:X60316) pleiotropic regulatory locus AbaA, OrfB (147 aa), fasta scores opt: 958 z-score: 1195.2 E(): 0 99.3% identity in 147 aa overlap. 3 4 5 5 [Reporter]SCO1703 (17O9)_PCR/7147 [Gene]SCO1703/6515 NC_003888 SCI30A.24, possible transcriptional regulator, len: 237aa; similar to many eg. SW:MERR_STRLI probable mercury resistance operon repressor from Streptomyces lividans (125 aa) fasta scores; opt: 144, z-score: 183.3, E(): 0.007, (38.2% identity in 89 aa overlap). Contains Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family. Contains possible helix-turn-helix motif (+3.92 SD) 37-58aa. 3 4 5 4 [Reporter]SCO1456 (18K9)_PCR/7146 [Gene]SCO1456/6514 NC_003888 SCL6.13c, unknown, len: 119 aa 3 4 5 3 [Reporter]SCO4991 (19G9)_PCR/7145 [Gene]SCO4991/6513 NC_003888 2SCK36.14, probable sugar transport integral membrane protein, len: 492 aa; similar to SW:GNTP_BACSU (EMBL:J02584) Bacillus subtilis gluconate permease GntP, 448 aa; fasta scores: opt: 988 Z-score: 1028.7 bits: 199.7 E(): 1.1e-49; 39.186% identity in 467 aa overlap. Contains Pfam match to entry PF02447 GntP_permease, GntP family permease and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Also contains possible hydrophobic membrane spanning regions 3 4 4 22 [Reporter]SCO4538 (1O1)_PCR/7142 [Gene]SCO4538/6512 NC_003888 2SCD4.09c, unknown, len: 111 aa. High content in aspartic acid, leucine and lysine amino acid residues. Highly hydrophilic 2 3 11 3 [Reporter]SCO1970 (19N14)_PCR/4758 [Gene]SCO1970/4339 NC_003888 SC3C9.05c, possible dioxygenase, len: 147 aa; similar to TR:Q9I0T8 (EMBL:AE004682) Pseudomonas aeruginosa probable ring-cleaving dioxygenase PA2546, 143 aa; fasta scores: opt: 178 Z-score: 231.1 bits: 48.7 E(): 2.9e-05; 33.333% identity in 135 aa overlap. Contains Pfam match to entry PF00903 Glyoxalase, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily 3 4 4 21 [Reporter]SCO5374 (2K1)_PCR/7141 [Gene]SCO5374/6511 NC_003888 2SC6G5.18, atpC, ATP synthase epsilon chain (EC 3.6.1.34), len: 124aa; similar to many eg. SW:P37812 (ATPE_BACSU) ATP synthase epsilon chain from Bacillus subtilis (132 aa) fasta scores; opt: 244, z-score: 304.5, E(): 1.7e-09, 34.7% identity in 124 aa overlap. Identical to SW:P50011 (ATPE_STRLI) ATP synthase epsilon chain from Streptomyces lividans. Contains Pfam match to entry PF00401 ATP-synt_DE, ATP synthase, Delta/Epsilon chain. 2 3 11 2 [Reporter]SCO5062 (20F14)_PCR/4757 [Gene]SCO5062/4338 NC_003888 SCBAC20F6.05c, hypothetical protein, len: 130 aa; similar to TR:Q9X8E7 (EMBL:AL049573) Streptomyces coelicolor hypothetical 16.4 kDa protein SCE39.24, 148 aa; fasta scores: opt: 257 Z-score: 327.8 bits: 66.5 E(): 1.1e-10; 39.394% identity in 132 aa overlap 3 4 4 20 [Reporter]SCO4607 (3G1)_PCR/7140 [Gene]SCO4607/6510 NC_003888 SCD39.07, nuoM2, NADH dehydrogenase subunit, len: 534 aa; similar to SW:NUOM_ECOLI (EMBL:X68301) Escherichia coli NADH dehydrogenase I chain M (EC 1.6.5.3) NuoM, 509 aa; fasta scores: opt: 721 z-score: 798.7 E(): 0; 33.9% identity in 528 aa overlap and to TR:Q9XAR6 (EMBL:AL078618) Streptomyces coelicolor SCD16A.09c, NuoM, NADH dehydrogenase subunit, 523 aa; fasta scores: opt: 462 z-score: 441.4 E(): 6e-19; 34.1% identity in 537 aa overlap. Contains Pfam match to entry PF00361 oxidored_q1, NADH-Ubiquinone/plastoquinone (complex I), various chains and possible hydrophobic membrane spanning regions 2 3 10 22 [Reporter]SCO5276 (1F10)_PCR/4755 [Gene]SCO5276/4337 NC_003888 2SC7G11.38, conserved hypothetical protein, len: 141 aa; similar to TR:CAB86112 (EMBL:AL163003) Streptomyces coelicolor hypothetical 16.0 kDa protein SCC24.18, 153 aa; fasta scores: opt: 178 z-score: 212.6 E(): 0.00023; 32.4% identity in 145 aa overlap 2 3 10 21 [Reporter]SCO7019 (2B10)_PCR/4754 [Gene]SCO7019/4336 NC_003888 SC1H10.08c, aml, secreted alpha-amylase (EC 3.2.1.1), len: 993. Identical to Streptomyces lividans TR:P96992(EMBL:Z86113) alpha-amylase, AmlC (993 aa), fasta scores opt: 6792 z-score: 6947.5 E(): 0 99.1% identity in 993 aa overlap. Also highly similar to the alpha-amylase domain of Paenibacillus polymyxa (Bacillus polymyxa) SW:AMYB_PAEPO(EMBL:M15817) beta/alpha-amylase precursor (1196 aa), fasta scores opt: 423 z-score: 430.0 E(): 1.6e-16 28.9% identity in 956 aa overlap. Contains a Pfam matches to entries PF00128 alpha-amylase, Alpha amylase and PF00041 fn3, Fibronectin type III domain. Also contains a possible N-terminal signal sequence. 2 3 10 20 [Reporter]SCO4719 (3N6)_PCR/4753 [Gene]SCO4719/4335 NC_003888 SCD31.44, rpsE, 30S ribosomal protein S5, len: 201 aa; identical to previously sequenced SW:RS5_STRCO (EMBL:X83011) Streptomyces coelicolor 30S ribosomal protein S5 RpsE, 201 aa. Contains Pfam match to entry PF00333 Ribosomal_S5, Ribosomal protein S5 and match to Prosite entry PS00585 Ribosomal protein S5 signature 2 3 10 19 [Reporter]SCO6623 (4J6)_PCR/4752 [Gene]SCO6623/4334 NC_003888 SC1F2.20, probable ATP/GTP binding protein, len: 731 aa; very weak similarity in C-terminus to a yeast ATP /GTP binding protein TR:Q12179 (EMBL:Z73601) Saccharomyces cerevisiae chromosome XVI ORF YPL245W (454 aa), Smith-Waterman scores;E: 3.1e-05, 20.4% identity in 499 aa overlap. Contains two TTA Leu codons: possible targets for action of bldA, PS00017 ATP /GTP-binding site motif A (P-loop) and a possible N-terminal signal sequence. Also similar to upstream gene SC1F2.19, E(): 0, 37.1% identity in 657 aa overlap 2 3 10 18 [Reporter]SCO5725 (5F6)_PCR/4751 [Gene]SCO5725/4333 NC_003888 SC3C3.11, unknown, len: 103 aa 1 2 17 5 [Reporter]SCO1545 (17M23)_PCR/2372 [Gene]SCO1545/2159 NC_003888 SCL11.01c, possible acetyltransferase (fragment), len: >81 aa; C-terminal part of TR:CAB70947 (EMBL:AL137778) S. coelicolor cosmid SCL2.35c putative acetyltransferase (fragment),SCL2.35c, possible acetyltransferase (partial), len: >225 aa; similar to TR:CAB50578 (EMBL:AJ248288) Pyrococcus abyssi N-terminal acetyltransferase, 172 aa; fasta scores: opt: 164 z-score: 202.4 E(): 0.0007; 36.0% identity in 89 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 2 3 10 17 [Reporter]SCO2041 (6B6)_PCR/4750 [Gene]SCO2041/4332 NC_003888 SC4G6.10c, possible membrane protein, len: 83aa; Contains possible membrane spanning hydrophobic regions. 1 2 17 4 [Reporter]SCP1.247c (18I23)_PCR/2371 [Gene]SCP1.247c/2158 NC_003888 SCP1.247c, unknown, len: 144aa; similar to TR:Q9RJS4 (EMBL:AL132707) hypothetical protein from Streptomyces coelicolor (127 aa) fasta scores; opt: 229, z-score: 300.2, E(): 3e-09, 37.3% identity in 110 aa overlap. 2 3 10 16 [Reporter]SCO3607 (7N2)_PCR/4749 [Gene]SCO3607/4331 NC_003888 SC66T3.18c, possible secreted protein, len: 489 aa; unknown function, similar to SW:YUAG_BACSU (EMBL:Z99119) Bacillus subtilis hypothetical protein (509 aa), fasta scores; opt: 618 z-score: 592.7 E(): 1.1e-25, 36.8% identity in 503 aa overlap. Also similar to many eukaryotic flotillins (epidermal surface antigens) e.g. TR:O75955 (EMBL:AF089750) Homo sapiens flotillin-1 (427 aa) (34.8% identity in 442 aa overlap). N-terminus is hydrophobic and may contitute a membrane anchor. Contains probable coiled-coil from 205 to 258 (54 residues) (Max score: 1.522, probability 0.97) and possible N-terminal region signal sequence peptide 1 1 22 22 [Reporter]SCO1100 (1E24)_PCR/483 [Gene]SCO1100/439 NC_003888 2SCG4.16, possible integral membrane protein, len: 192 aa. Contains possible hydrophobic membrane spanning regions 1 2 17 2 [Reporter]SCO7488 (20M19)_PCR/2369 [Gene]SCO7488/2157 NC_003888 SCBAC17A6.21c, possible integral membrane binding protein dependent transport protein, len: 343aa; similar to many eg. SW:Q04698 (MALC_STRPN) maltodextrin transport permease from Streptococcus pneumoniae (435 aa) fasta scores: opt: 376, Z-score: 423.7, 26.959% identity (29.153% ungapped) in 319 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, Prosite match to S00402 Binding-protein-dependent transport systems inner membrane comp sign. and multiple hydrophobic possible membrane spanning regions. 2 3 10 15 [Reporter]SCO5567 (8J2)_PCR/4748 [Gene]SCO5567/4330 NC_003888 SC7A1.11, possible DNA methylase, len: 195aa; similar to many hypotheticals some of which contain PS00092 N-6 Adenine-specific DNA methylases signature eg. SW:YHHF_ECOLI hypothetical protein from Escherichia coli (198 aa) fasta scores; opt: 340, z-score: 430.7, E(): 1.1e-16, (35.8% identity in 193 aa overlap). Contains PS00092 N-6 Adenine-specific DNA methylases signature although not in the expected N-terminal region. 1 1 22 21 [Reporter]SCO4092 (2A24)_PCR/482 [Gene]SCO4092/438 NC_003888 SCD25.28c, ATP-dependent helicase, len: 1327 aa. Highly similar to Escherichia coli SW:HRPA_ECOLI (EMBL; D42105) ATP-dependent helicase (1281 aa), fasta scores opt: 1818 z-score: 1967.4 E(): 0 61.9% identity in 1306 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 1 2 16 22 [Reporter]SCO1220 (1M15)_PCR/2367 [Gene]SCO1220/2156 NC_003888 2SCG58.20c, probable regulatory protein, len: 253 aa; similar to SW:ALGR_PSEAE (EMBL:M23230) Pseudomonas aeruginosa positive alginate biosynthesis regulatory protein AlgR, 248 aa; fasta scores: opt: 384 z-score: 457.3 E(): 5.3e-18; 36.5% identity in 255 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain 1 1 22 20 [Reporter]SCO1386 (3M20)_PCR/481 [Gene]SCO1386/437 NC_003888 SC1A8A.06c, possible integral membrane protein, len: 110 aa; similar to SW:Q50609 (Y0DJ_MYCTU) hypothetical protein from Mycobacterium tuberculosis (121 aa) fasta scores; opt: 478, z-score: 600.3, E(): 5.2e-26, 67.3% identity in 110 aa overlap. Contains possible hydrophobic membrane spannig regions 1 2 16 21 [Reporter]SCO5522 (2I15)_PCR/2366 [Gene]SCO5522/2155 NC_003888 SC1C2.03, leuB, probable 3-isopropylmalate dehydrogenase, len: 347 aa; similar to e.g. LEU3_CORGL 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (340 aa), fasta scores; opt: 1450 z-score: 1318.1 E(): 0, 68.0% identity in 338 aa overlap. Contains PS00470 Isocitrate and isopropylmalate dehydrogenases signature and Pfam match to entry PF00180 isodh, Isocitrate and isopropylmalate dehydrogenases, score 356.60, E-value 1.3e-105 1 1 22 19 [Reporter]SCO7625 (4I20)_PCR/480 [Gene]SCO7625/436 NC_003888 SC2H2.23, probable monooxygenase, len: 373 aa; similar to SW:TETX_BACFR (EMBL:M37699) Bacteroides fragilis tetracycline resistance protein TetX, 388 aa; fasta scores: opt: 598 z-score: 663.7 E(): 2e-29; 31.3% identity in 367 aa overlap. Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase 1 2 16 20 [Reporter]SCO7792 (3E15)_PCR/2365 [Gene]SCO7792/2154 NC_003888 SC10B8A.02c, unknown, doubtful CDS, len: 106 aa 1 1 22 18 [Reporter]SCO7111 (5E20)_PCR/479 [Gene]SCO7111/435 NC_003888 SC4B10.12c, probable hydroperoxide resistance protein, len: 137 aa; similar to SW:OHR_XANCH (EMBL:AF036166) Xanthomonas campestris organic hydroperoxide resistance protein Ohr, 142 aa; fasta scores: opt: 341 z-score: 437.5 E(): 6.6e-17; 47.1% identity in 138 aa overlap 1 2 16 19 [Reporter]SCO4817 (4A15)_PCR/2364 [Gene]SCO4817/2153 NC_003888 SC2A6.02c, possible serine/threonine protein kinase, len: 452 aa; similar to TR:CAB82017 (EMBL:AL161755) Streptomyces coelicolor serine/threonine protein kinase SCD63.10, 380 aa; fasta scores: opt: 297 z-score: 312.5 E(): 6.3e-10; 28.8% identity in 379 aa overlap and to Streptomyces coelicolor SC2A6.05c, 712 aa; fasta scores: opt: 1410 z-score: 1253.9 E(): 0; 68.7% identity in 339 aa overlap. Contains Pfam matches to entries PF00069 pkinase, Eukaryotic protein kinase domain and PF01471 PG_binding_1, Putative peptidoglycan binding domain. Contains also possible hydrophobic membrane spanning regions 1 1 22 17 [Reporter]SCO7567 (6A20)_PCR/478 [Gene]SCO7567/434 NC_003888 SC5F1.21, probable oligopeptide ABC transporter ATP-binding protein, len: 316 aa; similar to SW:OPPF_BACSU (EMBL:X56347) Bacillus subtilis oligopeptide transport ATP-binding protein OppF, 308 aa; fasta scores: opt: 811 z-score: 836.7 E(): 0; 46.9% identity in 273 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 2 16 18 [Reporter]SCO2102 (5M11)_PCR/2363 [Gene]SCO2102/2152 NC_003888 SC4A10.35c, possible membrane protein, len: 330 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. The C-terminal half is Gln-rich. Contains hydrophobic, possible membrane-spanning regions in the N-terminus 1 1 22 16 [Reporter]SCO2114 (7M16)_PCR/477 [Gene]SCO2114/433 NC_003888 SC6E10.08c, hypothetical protein, len: 83 aa; unknown function, similar to part of many nitrate or nitrite reductases e.g. SW:NIR_EMENI (EMBL:M58289), NiiA, Emericella nidulans nitrite reductase (1104 aa), fasta scores; opt: 137 z-score: 178.6 E(): 0.013, 38.0% identity in 50 aa overlap 1 2 16 17 [Reporter]SCO6526 (6I11)_PCR/2362 [Gene]SCO6526/2151 NC_003888 SC5C7.11, unknown, len: 133 aa 1 1 22 15 [Reporter]SCO2006 (8I16)_PCR/476 [Gene]SCO2006/432 NC_003888 SC7H2.20, unknown, len: 181aa; 1 2 16 16 [Reporter]SCO5698 (7E11)_PCR/2361 [Gene]SCO5698/2150 NC_003888 SC5H4.22, hypothetical protein, len: 180 aa; similar to TR:O69563 (EMBL:AL022602) Mycobacterium leprae hypothetical 23.9 kD protein MLCB268.13, 210 aa; fasta scores: opt: 243 z-score: 313.1 E(): 5.6e-10; 29.1% identity in 203 aa overlap 1 1 22 14 [Reporter]SCO7543 (9E16)_PCR/475 [Gene]SCO7543/431 NC_003888 SC8G12.19, possible Crp-family transcriptional regulator, len: 424 aa. Similar to several including: Bacillus subtilis SW:XYLR_BACSU (EMBL:M27248) xylose repressor (384 aa), fasta scores opt: 339 z-score: 347.1 E(): 7.1e-12 23.3% identity in 390 aa overlap and Streptomyces coelicolor TR:O50502 (EMBL:AL009199) probable transcriptional repressor protein, SC7B7.05 (403 aa), fasta scores opt: 691 z-score: 697.8 E(): 2.1e-31 33.9% identity in 387 aa overlap. Contains a Prosite hit to PS01125 ROK family signature and Pfam hits to PF00480 ROK, ROK family and PF00325 crp, Bacterial regulatory proteins, crp family with the putative helix-turn-helix motif situated between residues 32..53 (+4.61 SD). 1 1 22 13 [Reporter]SCO2438 (10A16)_PCR/474 [Gene]SCO2438/430 NC_003888 SCC24.09, possible secreted protein, len: 468 aa; similar to various hypothetical proteins, e.g. TR:O86550 (EMBL:AL031350) Streptomyces coelicolor hypothetical 50.7 kD protein SC1F2.13c, 476 aa; fasta scores: opt: 931 z-score: 995.7 E(): 0; 43.2% identity in 458 aa overlap. Contains possible N-terminal region signal peptide sequence 3 4 4 19 [Reporter]SCO6156 (4C1)_PCR/7139 [Gene]SCO6156/6509 NC_003888 SC1A9.20, possible regulatory protein, len: 135aa; similar to TR:O69204 (EMBL:U33059) hypothetical protein from Amycolatopsis mediterranei S699 (144 aa) fasta scores; opt:225, z-score:335.1. E(): 2.2e-11, (41.7% identity in 115 aa overlap). Also similar to TR:Q53897 (EMBL:X60316) AbaA regulatory locus for antibiotic production in Streptomyces coelicolor (192 aa), fasta scores; opt: 155, z-score: 284.6, E(): 1.4e-08, (33.3% identity in 120 aa overlap) 3 4 4 18 [Reporter]SCO6230 (4O21)_PCR/7138 [Gene]SCO6230/6508 NC_003888 SC2H4.12c, probable sugar transport system permease protein, len: 324 aa; similar to many e.g. AMYD_THETU potential starch degradation products transport system permease (292 aa), fasta scores; opt: 479 z-score: 571.8 E(): 1.3e-24, 28.8% identity in 281 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 58.80, E-value 1.2e-13 3 4 4 17 [Reporter]SCO7050 (5K21)_PCR/7137 [Gene]SCO7050/6507 NC_003888 SC4G1.16c, probable D-alanyl-D-alanine carboxypeptidase, len: 413 aa; similar to TR:CAB89066 (EMBL:AL353872) Streptomyces coelicolor putative D-alanyl-D-alanine carboxypeptidase SC5G8.15c, 424 aa; fasta scores: opt: 704 z-score: 719.2 E(): 1.4e-32; 47.7% identity in 390 aa overlap and to SW:DACF_BACSU (EMBL:M85047) Bacillus subtilis penicillin-binding protein DacF precursor, 389 aa; fasta scores: opt: 330 z-score: 341.5 E(): 1.5e-11; 27.1% identity in 262 aa overlap. Contains Pfam match to entry PF00768 Peptidase_S11, D-alanyl-D-alanine carboxypeptidase 3 4 4 16 [Reporter]SCO7582 (6G21)_PCR/7136 [Gene]SCO7582/6506 NC_003888 SC5F1.36c, hypothetical protein, len: 405 aa; low similarity to SW:Y812_METJA (EMBL:U67525) Methanococcus jannaschii hypothetical protein MJ0812, 373 aa; fasta scores: opt: 567 z-score: 644.6 E(): 2.2e-28; 33.1% identity in 311 aa overlap 3 4 4 15 [Reporter]SCO7418 (7C21)_PCR/7135 [Gene]SCO7418/6505 NC_003888 SC6D11.14c, possible cytochrome P450-family protein, len: 413 aa. Similar to many members of the cytochrome P450 family including: Saccharopolyspora erythraea (Streptomyces erythraeus) SW:CPXK_SACER(EMBL:M83110) cytochrome P450 107B1 (405 aa), fasta scores opt: 491 z-score: 571.3 E(): 2.1e-24 28.9% identity in 395 aa overlap and Streptomyces coelicolor TR:CAB61278(EMBL:AL132991) putative cytochrome P450, SCF55.08c (420 aa), fasta scores opt: 1375 z-score: 1593.6 E():0 51.7% identity in 404 aa overlap. 3 4 4 14 [Reporter]SCO2020 (8O17)_PCR/7134 [Gene]SCO2020/6504 NC_003888 SC7H2.34, unknown, len: 299aa; 3 4 4 13 [Reporter]SCO6131 (9K17)_PCR/7133 [Gene]SCO6131/6503 NC_003888 SC9B2.18c, probable carboxypeptidase, len: 451aa; similar to SW:DAC_ACTSP D-alanyl-D-alanine carboxypeptidase precursor from Actinomadura sp. (strain R39) (538 aa) fasta scores; opt: 399, z-score: 430.8, E(): 1e-16, (30.9% identity in 512 aa overlap) and PBP4_ECOLI penicillin-binding protein 4 precursor (PBP-4) (D-alanyl-D-alanine carboxypeptidase) from Escherichia coli (477 aa) fasta scores; opt: 383, z-score: 414.4, E(): 8.6e-16, (29.4% identity in 445 aa overlap). Contains probable hydrophobic membrane spanning region 3 4 4 11 [Reporter]SCO4356 (11C17)_PCR/7131 [Gene]SCO4356/6502 NC_003888 SCD19.11c, unknown, len: 229 aa 2 3 10 14 [Reporter]SCO5677 (9F2)_PCR/4747 [Gene]SCO5677/4329 NC_003888 SC5H4.01, possible ATP/GTP binding protein (putative membrane protein) (fragment), len: >350 aa,SC8B7.01c, probable ATP/GTP binding protein (putative membrane protein), partial CDS, len: >604 aa. Contains PS00017 ATP/GTP-binding site m otif A (P-loop), and two possible transmembrane domains at C-terminus 3 4 4 10 [Reporter]SCO3518 (12O13)_PCR/7130 [Gene]SCO3518/6501 NC_003888 SCE134.19, unknown, len: aa; 2 3 10 13 [Reporter]SCO6483 (10B2)_PCR/4746 [Gene]SCO6483/4328 NC_003888 SC9C7.19, probable efflux protein, len: 815aa; similar to many, several of which are involved in toxin /antibiotic efflux eg. TR:O69070 (EMBL:AF061335) oxytetracycline exporter from Streptomyces rimosus (536 aa) fasta scores; opt: 1020, z-score: 879.9, E(): 0, (36.9% identity in 542 aa overlap). 3 4 4 9 [Reporter]SCO1889 (13K13)_PCR/7129 [Gene]SCO1889/6500 NC_003888 SCI7.07c, probable dihydropicolinate synthase, len: 302 aa; similar to many e.g. SW:DAPA_BACSU dihydropicolinate synthase from Bacillus subtilis (290 aa) fasta scores; opt: 280, z-score: 323.5, E(): 1.1e-10, (28.2% identity in 284 aa overlap). Contains Pfam match to entry PF00701 DHDPS, Dihydrodipicolinate synthetase family. 2 3 10 12 [Reporter]SCO2765 (10N22)_PCR/4745 [Gene]SCO2765/4327 NC_003888 SCC57A.36, unknown, len: 123 aa. 2 3 10 11 [Reporter]SCO4260 (11J22)_PCR/4744 [Gene]SCO4260/4326 NC_003888 SCD49.01c, hypothetical protein (fragment), len: >115 aa; N-terminal part of TR:CAB77354 (EMBL:AL160331) Streptomyces coelicolor hypothetical 24.5 kD protein (fragment) SCD8A.33c, 224 aa and similar to TR:P96262 (EMBL:Z84724) Mycobacterium tuberculosis hypothetical 7.4 kD protein MTCY22G10.13, 68 aa; fasta scores: opt: 71 z-score: 131.0 E(): 8; 41.0% identity in 39 aa overlap,SCD8A.33c, unknown (fragment), len: >224 aa 2 3 10 10 [Reporter]SCO3387 (12F22)_PCR/4743 [Gene]SCO3387/4325 NC_003888 SCE126.05, possible transcriptional regulator, len: 368aa; similar to many in a limited region which corresponds to the probable DNA-binding site eg. SW:SMTB_SYNP7 SmtB transcriptional repressor from Synechococcus sp. (Strain PCC7942) (122 aa) fasta scores; opt: 169, z-score: 204.8, E(): 0.00043, (37.0% identity in 92 aa overlap). Contains Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family. 2 3 10 9 [Reporter]SCO1902 (13B22)_PCR/4742 [Gene]SCO1902/4324 NC_003888 SCI7.20, unknown, hydrophilic, len: 129aa; 2 3 10 8 [Reporter]SCO1526 (14N18)_PCR/4741 [Gene]SCO1526/4323 NC_003888 SCL2.16c, possible acyltransferase, len: 311 aa; similar to TR:Q9Z983 (EMBL:AE001596) Chlamydia pneumoniae acyltransferase HtrB, 467 aa; fasta scores: opt: 243 z-score: 289.8 E(): 9.4e-09; 29.0% identity in 238 aa overlap and to SW:HTRB_ECOLI (EMBL:X61000) Escherichia coli lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) HtrB, 306 aa; fasta scores: opt: 130 z-score: 159.2 E(): 0.18; 23.4% identity in 286 aa overlap 1 2 16 14 [Reporter]SCO6977 (9M7)_PCR/2359 [Gene]SCO6977/2149 NC_003888 SC8F11.03c, unknown, len: 298 aa. weakly similar to Streptomyces coelicolor TR:CAB59608 (EMBL:AL132662) putative histidine kinase protein, SCF11.31C (442 aa), fasta scores opt: 131 z-score: 160.7 E(): 0.16 30.2% identity in 265 aa overlap. 2 3 10 7 [Reporter]SCO3424 (15J18)_PCR/4740 [Gene]SCO3424/4322 NC_003888 SCE9.31c, possible regulator, len: 83 aa; similar to TR:Q53896 (EMBL:X60316), abaA, Streptomyces coelicolor ORFD from AbaA regulatory locus for antibiotic production (75 aa), fasta scores; opt: 307 z-score: 422.3 E(): 3.4e-16, 64.9% identity in 74 aa overlap and to TR:Q53842 (EMBL:U28930), BldB, S.coelicolor developmental protein (98 aa) (41.8% identity in 67 aa overlap) 1 2 16 13 [Reporter]SCO2505 (10I7)_PCR/2358 [Gene]SCO2505/2148 NC_003888 SCC121.08, probable ABC-transporter metal-binding lipoprotein, len: 328 aa; similar to TR:O05703 (EMBL:Z71552) Streptococcus pneumoniae AdcA protein (fragment), 423 aa; fasta scores: opt: 551 z-score: 603.5 E(): 3.2e-26; 39.7% identity in 305 aa overlap and to SW:ZNUA_ECOLI (EMBL:U38702) Escherichia coli, high-affinity zinc uptake system protein ZnuA precursor ZnuA, 328 aa; fasta scores: opt: 294 z-score: 327.0 E(): 8e-11; 26.5% identity in 283 aa overlap. Contains Pfam match to entry PF01297 Lipoprotein_4 and match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide sequence 2 3 10 6 [Reporter]SCO3883 (16F18)_PCR/4739 [Gene]SCO3883/4321 NC_003888 StH24.05, probable membrane protein, len: 431 aa; previously sequenced as TR:O54569 (EMBL:Y16311) Streptomyces coelicolor inner membrane protein (431 aa). Similar to many e.g. SW:SP3J_BACSU (EMBL:Z14225), SpoIIIJ, Bacillus subtilis stage III sporulation protein J precursor (259 aa), fasta scores; opt: 299 z-score: 305.0 E(): 1.1e-09, 30.9% identity in 236 aa overlap. Also similar to TR:O04665 (EMBL:U89272), albino3, Arabidopsis thaliana chloroplast membrane protein (462 aa) (27.7% identity in 364 aa overlap) and many bacterial hypothetical 60 kD membrane proteins e.g. SW:60IM_HAEIN (EMBL:U32781) Haemophilus influenzae hypothetical protein (541 aa) (33.6% identity in 253 aa overlap). Contains two hydrophobic, probable membrane-spanning regions 1 1 22 12 [Reporter]SCO4560 (11M12)_PCR/473 [Gene]SCO4560/429 NC_003888 SCD16A.23, possible polypetide deformylase, len: 208aa; similar to many eg. TR:P96275 (EMBL:Z84724) hypothetical protein from Mycobacterium tuberculosis (197 aa) fasta scores; opt: 425, z-score: 513.1, E(): 3e-21, (48.0% identity in 171 aa overlap) and SW:DEF_ECOLI polypetide deformylase from Escherichia coli (168 aa) fasta scores; opt: 373, z-score: 452.6, E(): 7e-18, (35.6% identity in 174 aa overlap). Contains Pfam match to entry PF01327 Pep_deformylase, Polypeptide deformylase. 1 2 16 12 [Reporter]SCO2539 (11E7)_PCR/2357 [Gene]SCO2539/2147 NC_003888 SCC77.06, Era-like GTP-binding protein, len: 320 aa. Highly similar to many Era GTP-binding proteins essential for cellular proliferation: Escherichia coli SW:ERA_ECOLI(EMBL:M14658) GTP-binding protein Era (301 aa), fasta scores opt: 434 z-score: 499.6 E(): 1.9e-20 36.4% identity in 302 aa overlap and Mycobacterium tuberculosis SW:ERA_MYCTU(EMBL:Z95208) GTP-binding protein Era homologue (300 aa), fasta scores opt: 1264 z-score: 1440.4 E(): 0 64.1% identity in 306 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 2 3 10 5 [Reporter]SCO0398 (17B18)_PCR/4738 [Gene]SCO0398/4320 NC_003888 SCF62.24, possible glycosyl transferase, len: 315 aa. Similar to several e.g. Rickettsia prowazekii TR:Q9ZD96 (EMBL:AJ235271) glycosyl transferase (280 aa), fasta scores opt: 272 z-score: 326.2 E(): 8.1e-11 29.9% identity in 214 aa overlap and Neisseria gonorrhoeae TR:Q50949 (EMBL:U14554) glycosyl transferase (337 aa), fasta scores opt: 234 z-score: 280.4 E(): 2.9e-08 27.8% identity in 248 aa overlap. Contains a Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases 1 1 22 11 [Reporter]SCO4264 (12I12)_PCR/472 [Gene]SCO4264/428 NC_003888 SCD86A.01c, aminoglycoside phosphotransferase (fragment), len: >101 aa; highly similar to SW:HUR_STRAU (EMBL:M81739) Streptomyces aureofaciens hydroxyurea phosphotransferase (EC 2.7.-.-) Hur, 340 aa; fasta scores: opt: 413 z-score: 532.0 E(): 3.7e-22; 63.0% identity in 100 aa overlap,SCD49.05c, probable aminoglycoside phosphotransferase (fragment), len: >223 aa; highly similar to SW:HUR_STRAU (EMBL:M81739) Streptomyces aureofaciens hydroxyurea phosphotransferase (EC 2.7.-.-) Hur, 340 aa; fasta scores: opt: 900 z-score: 1065.5 E(): 0; 61.1% identity in 221 aa overlap 1 2 16 10 [Reporter]SCO3043 (13M3)_PCR/2355 [Gene]SCO3043/2146 NC_003888 SCE34.24, conserved hypothetical protein, len: 472 aa; similar to TR:O68907 (EMBL:AF05830) Streptomyces roseofulvus FrnA, 770 aa; fasta scores: opt: 1249 z-score: 1391.4 E(): 0; 52.0% identity in 429 aa overlap, to Streptomyces coelicolor SCE34.26, 507 aa; fasta scores: opt: 2161 z-score: 2203.4 E(): 0; 70.9% identity in 471 aa overlap, to SCE34.23, 404 aa; fasta scores: opt: 666 z-score: 682.6 E(): 1.6e-32; 38.0% identity in 324 aa overlap, to SCE34.27 (fragment), >356 aa; fasta scores: opt: 659 z-score: 676.4 E(): 3.7e-32; 39.9% identity in 348 aa overlap and to SCE35.25, 507 aa; fasta scores: opt: 644 z-score: 658.7 E(): 3.5e-31; 34.0% identity in 509 aa overlap 1 1 22 10 [Reporter]SCO3014 (13E12)_PCR/471 [Gene]SCO3014/427 NC_003888 SCE33.16c, possible translation initiation factor, len: 380 aa; similar to TR:Q9ZUG4 (EMBL:AC005970) Arabidopsis thaliana putative translation initiation factor EIF-2B alpha subunit T6P5.3, 365 aa; fasta scores: opt: 631 z-score: 658.4 E(): 3.2e-29; 37.6% identity in 338 aa overlap. Contains Pfam match to entry PF01008 IF-2B, Initiation factor 2 subunit family 1 2 16 9 [Reporter]SCO0174 (14I3)_PCR/2354 [Gene]SCO0174/2145 NC_003888 SCJ1.23c, possible DNA-binding protein, len: 323 aa. Similar in parts to many, e.g. Mycobacterium tuberculosis TR:O53625 (EMBL; AL021428) hypothetical 16.6 KD protein MTV030.24 (152 aa), fasta scores opt: 224 z-score: 258.5 E(): 4.9e-07 34.3% identity in 134 aa overlap and Mycobacterium tuberculosis SW:YU07_MYCTU (EMBL; Z77163) hypothetical 8.6 KD protein MTCY339.07C (80 aa), fasta scores opt: 182 z-score: 216.4 E(): 0.00011 42.3% identity in 71 aa overlap. Also similar to TR:Q9RJ05 (EMBL:AL109962) Streptomyces coelicolor possible DNA-binding protein SCJ1.09c, 233 aa; fasta scores: opt: 516 Z-score: 566.6 E(): 6.3e-24; 37.615% identity in 218 aa overlap. Contains a Pfam match to entry PF01381 HTH_3, Helix-turn-helix and a possible helix-turn-helix motif at residues 127..148 (+2.82 SD) 1 1 22 9 [Reporter]SCO4871 (14A12)_PCR/470 [Gene]SCO4871/426 NC_003888 SCK20.12, probable tetR-family transcriptional regulator, len: 216 aa; similar to SW:TER1_ECOLI (EMBL:X61367) Escherichia coli tetracycline repressor protein class A TetR, 216 aa; fasta scores: opt: 437 z-score: 544.2 E(): 8.9e-23; 43.2% identity in 199 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 30..51 (+5.12 SD) 1 2 16 8 [Reporter]SCO0582 (15E3)_PCR/2353 [Gene]SCO0582/2144 NC_003888 SCF55.06, possible transcriptional regulator, len: 253 aa; similar to SW:PDHR_ECOLI (EMBL:V01498) Escherichia coli, pyruvate dehydrogenase complex repressor PdhR, opt: 292 z-score: 349.5 E(): 4.3e-12; 28.6% identity in 220 aa overlap. Contains match to Pfam entry PF00392 gntR, Bacterial regulatory proteins, gntR family 1 1 22 8 [Reporter]SCO0246 (15M8)_PCR/469 [Gene]SCO0246/425 NC_003888 SCJ9A.25c, possible integral membrane protein, len: 445aa; similar to many hypothetical proteins eg. SW:Y0D2_MYCTU from Mycobacterium tuberculosis (455 aa) fasta scores; opt: 925, z-score: 997.3, E(): 0, (39.4% identity in 411 aa overlap). Contains two Pfam matches to entry PF00571 CBS, CBS domain and a Pfam match to entry PF01595 DUF21. Also contains possible membrane spanning hydrophobic regions in the N-terminal region. 1 2 16 7 [Reporter]SCO0808 (16A3)_PCR/2352 [Gene]SCO0808/2143 NC_003888 SCF43.19c, possible secreted solute-binding lipoprotein, len: 360 aa. Similar to many bacterial extracellular solute-binding proteins including: Bacillus subtilis SW:RBSB_BACSU(EMBL:Z92953) D-ribose-binding protein precursor, RbsB (305 aa), fasta scores opt: 234 z-score: 252.1 E(): 1.2e-06 24.9% identity in 277 aa overlap and Streptomyces coelicolor TR:CAB56679(EMBL:AL121596) possible secreted solute-binding lipoprotein SCF51A.27 (359 aa), fasta scores opt: 1487 z-score: 1547.8 E(): 0 63.1% identity in 360 aa overlap. Contains an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. 1 1 22 7 [Reporter]SCO0272 (16I8)_PCR/468 [Gene]SCO0272/424 NC_003888 SCF1.14, possible binding-protein-dependent transport protein, len: 296aa; similar to many predicted membrane transporters eg. TR:O53483 (EMBL:AL021899) sugar transport protein from Mycobacterium tuberculosis (280 aa) fasta scores; opt: 594, z-score: 708.0, E(): 4.5e-32, (34.6% identity in 283 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and possible membrane spanning hydrophobic regions. 1 2 16 6 [Reporter]SCO1721 (16M23)_PCR/2351 [Gene]SCO1721/2142 NC_003888 SCI11.10c, hypothetical protein, len: 89 aa; unknown function, possible CDS suggested by GC frameplot 1 1 22 6 [Reporter]SCO3560 (17E8)_PCR/467 [Gene]SCO3560/423 NC_003888 SCH5.23, possible ATP-binding protein, len: 329aa; Contains PS00017 ATP/GTP-binding site motif A (P-loop). Similar to other proposed ATP-binding proteins of undefined function eg. SW:Y823_METJA hypothetical ATP-binding protein from Methanococcus jannashii (257 aa) fasta scores; opt: 237, z-score: 279.6, E(): 2.9e-08, (30.5% identity in 256 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). 1 2 16 5 [Reporter]SCO3765 (17I23)_PCR/2350 [Gene]SCO3765/2141 NC_003888 SCH63.12c, possible integral membrane protein, len: 459 aa; similar to TR:CAC01475 (EMBL:AL391014) Streptomyces coelicolor putative integral membrane protein 2SCG1.26, 468 aa; fasta scores: opt: 1879 z-score: 2014.4 E(): 0; 67.8% identity in 441 aa overlap. Contains Pfam match to entry PF01595 DUF21, Domain of unknown function DUF21 and 2x matches to PF00571 CBS, CBS domain. Also contains possible hydrophobic membrane spanning regions 1 1 22 5 [Reporter]SCO0223 (18A8)_PCR/466 [Gene]SCO0223/422 NC_003888 SCJ9A.02, improbable CDS, function unknown, len: 38aa; predicted by Hidden Markov Model and amino acid usage. 1 2 16 4 [Reporter]SCO0248 (18E23)_PCR/2349 [Gene]SCO0248/2140 NC_003888 SCJ9A.27c, unknown, len: 195aa; prediction based on Hidden Markov Model and amino acid usage. 1 1 22 4 [Reporter]SCO4054 (19M4)_PCR/465 [Gene]SCO4054/421 NC_003888 2SCD60.20, possible integral membrane protein, len: 228 aa. Contains possible hydrophobic membrane spanning regions 1 1 22 3 [Reporter]SCP1.238 (20I4)_PCR/464 [Gene]SCP1.238/420 NC_003888 SCP1.238, mmyJ, hypothetical protein, len: 111aa; previously sequenced and annotated as TR:Q52517 (EMBL:AJ276673). Similar to many others eg. TR:O31480 (EMBL:Z99106) hypothetical protein from Bacillus subtilis (104 aa) fasta scores; opt: 279, z-score: 375.5, E(): 1.9e-13, 45.4% identity in 97 aa overlap. 2 3 10 4 [Reporter]SCP1.12c (18N14)_PCR/4737 [Gene]SCP1.12c/4319 NC_003888 SCP1.12c, doubtful CDS, unknown, len: 151aa; 2 3 10 3 [Reporter]SCO1369 (19J14)_PCR/4736 [Gene]SCO1369/4318 NC_003888 SC10A9.11, probable two component system histidine kinase, len: 404 aa; highly similar to TR:Q9RIM1 (EMBL:Y14336) Streptomyces reticuli histidine autokinase SenS, 398 aa; fasta scores: opt: 911 z-score: 966.5 E(): 0; 46.0% identity in 404 aa overlap. Contains Pfam match to entry PF00512 signal, Histidine kinase and N-terminal region possible hydrophobic membrane spanning regions 2 3 10 2 [Reporter]SCO7501 (20B14)_PCR/4735 [Gene]SCO7501/4317 NC_003888 SCBAC17A6.34, possible hydrolase, len: 491 aa; similar to many e.g. TR:P82594 (EMBL:AB023626) alpha-L-arabinofuranosidase from Streptomyces chartreusis GS901 (328 aa) fasta scores: opt: 386, Z-score: 429.4, 31.333% identity (33.571% ungapped) in 300 aa overlap. Contains a possible hydrophobic membrane spanning region 2 3 9 22 [Reporter]SCO5271 (1B10)_PCR/4733 [Gene]SCO5271/4316 NC_003888 2SC7G11.33, hypothetical protein, len: 1096 aa; similar to TR:CAB86115 (EMBL:AL163003) Streptomyces coelicolor hypothetical 145.7 kDa protein SCC24.21, 1349 aa; fasta scores: opt: 758 z-score: 652.1 E(): 7.4e-29; 29.8% identity in 1157 aa overlap. Contains a possible coiled-coil region at approx. residues 1011..1029 2 3 9 21 [Reporter]SCO1852 (2N6)_PCR/4732 [Gene]SCO1852/4315 NC_003888 SCI8.37, cobB, cobyrinic acid A,C-diamide synthase, len: 486 aa. Highly similar to many including: Pseudomonas denitrificans SW:COBB_PSEDE (EMBL: M59236) cobyrinic acid A,C-diamide synthase (433 aa), fasta scores opt: 635 z-score: 610.8 E(): 1.2e-26 39.1% identity in 465 aa overlap and Mycobacterium tuberculosis TR: O05811 (EMBL: Z95207) CobB (457 aa), fasta scores opt: 752 z-score: 721.4 E(): 8.2e-33 53.9% identity in 486 aa overlap. Contains a Pfam match to entry PF01656 CBIA, Cobyrinic acid A,C-diamide synthase and a Prosite hit to PS00237 G-protein coupled receptors signature. 2 3 9 20 [Reporter]SCO4654 (3J6)_PCR/4731 [Gene]SCO4654/4314 NC_003888 SCD82.26, rpoB, DNA-directed RNA polymerase beta chain, len: 1161 aa; highly similar to SW:RPOB_MYCTU (EMBL:L27989) Mycobacterium tuberculosis DNA-directed RNA polymerase beta chain (EC 2.7.7.6) RpoB, 1178aa; fasta scores: opt: 5849 z-score: 6361.5 E(): 0; 75.7% identity in 1169 aa overlap. Contains Pfam match to entry PF00562 RNA_pol_B, RNA polymerase beta subunit and match ot Prosite entry PS01166 RNA polymerases beta chain signature 2 3 9 19 [Reporter]SCO6044 (4F6)_PCR/4730 [Gene]SCO6044/4313 NC_003888 SC1B5.04c, possible integral membrane protein, len: 265 aa 1 2 16 3 [Reporter]SCP1.295 (19A23)_PCR/2348 [Gene]SCP1.295/2139 NC_003888 SCP1.295, possible regulatory protein, len: 147aa; similar to others from Streptomyces coelicolor eg. TR:Q53897 (EMBL:X60316) AbaA pleiotropic regulatory protein from Streptomyces coelicolor (192 aa) fasta scores; opt: 112, z-score: 149.1, E(): 0.77, 31.7% identity in 120 aa overlap. Contains TTA encoded leucine at residue 11, possible target for bldA regulation. 2 3 9 18 [Reporter]SCO5720 (5B6)_PCR/4729 [Gene]SCO5720/4312 NC_003888 SC3C3.06c, possible membrane protein, len: 422 aa; contains two possible transmembrane domains at N-terminus 1 2 16 2 [Reporter]SCO3053 (20I19)_PCR/2347 [Gene]SCO3053/2138 NC_003888 SCBAC19G2.08c, possible secreted esterase, len: 706 aa: similar to many from streptomycetes e.g. TR:Q54345 (EMBL:Z50108) FusH fusidic acid inactivating esterase from Streptomyces lividans 66 (509 aa) fasta scores; opt: 821, Z-score: 876.2, 36.782% identity (40.592% ungapped) in 522 aa overlap and TR:Q9ADF5 (EMBL:AL589708) secreted esterase FusH from Streptomyces coelicolor (520 aa) fasta scores: opt: 816 Z-score: 872.0 E(): 6.4e-41; 36.154% identity in 520 aa overlap. Contains a possible non-cleavable N-terminal signal sequence; Pfam match to entry PF01453 Agglutinin, Lectin (probable mannose binding); Pfam match to entry PF00089 trypsin, Trypsin and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. C-terminal region contains a degenerate repeat sequence. Contains possible N-terminal region signal peptide sequence 2 3 9 17 [Reporter]SCO6656 (6N2)_PCR/4728 [Gene]SCO6656/4311 NC_003888 SC5A7.06c, unknown, len: 409 aa; similar to several hypothetical protein e.g. Y07V_MYCTU MTCY02B10.31C (377 aa), fasta sores; opt: 675 z-score: 725.7 E(): 3.5e-33, 40.9% identity in 340 aa overlap, and more distantly to several penicillin binding proteins e.g. PBPE_BACSU penicillin-binding protein 4* (451 aa), fasta sores; opt: 148 z-score: 268.0 E(): 1.1e-07, 24.9% identity in 350 aa overlap 1 1 21 22 [Reporter]SCO1096 (1A24)_PCR/461 [Gene]SCO1096/419 NC_003888 2SCG4.12, unknown, len: 64 aa 1 2 15 22 [Reporter]SCO1215 (1I15)_PCR/2345 [Gene]SCO1215/2137 NC_003888 2SCG58.15, possible integral membrane protein, len: 316 aa; similar to SW:CTAG_BACSU (EMBL:Z98682) Bacillus subtilis CtaG protein, 297 aa; fasta scores: opt: 139 z-score: 169.9 E(): 0.053; 24.7% identity in 292 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 9 16 [Reporter]SCO3603 (7J2)_PCR/4727 [Gene]SCO3603/4310 NC_003888 SC66T3.14c, probable Na(+)/H(+) antiporter, len: 514 aa; weakly similar to SW:NAPA_ENTHR (EMBL:M81961), napA, Enterococcus hirae probable Na(+)/H(+) antiporter (383 aa), fasta scores; opt: 270 z-score: 297.1 E(): 3.2e-09, 24.9% identity in 305 aa overlap and to many putative Na(+)/H(+) antiporters. Contains hydrophobic, possible membrane-spanning regions. Weakly similar to TR:O69958 (EMBL:AL022268) S.coelicolor possible transmembrane transport protein (411 aa) (25.4% identity in 413 aa overlap). Contains Pfam match to entry PF00999 Na_H_Exchanger, Sodium/hydrogen exchanger family 1 1 21 21 [Reporter]SCO4643 (2M20)_PCR/460 [Gene]SCO4643/418 NC_003888 SCD82.14, murB, UDP-N-acetylenoylpyruvoylglucosamine reductase, len: 383 aa; similar to SW:MURB_ECOLI (EMBL:M10123) Escherichia coli UDP-N-acetylenoylpyruvoylglucosamine reductase (EC 1.1.1.158) MurB, 342 aa; fasta scores: opt: 326 z-score: 374.6 E(): 1.9e-13; 31.1% identity in 347 aa overlap 1 2 15 21 [Reporter]SCO5472 (2E15)_PCR/2344 [Gene]SCO5472/2136 NC_003888 SC2A11.06c, gcvT, probable aminomethyltransferase (glycine cleavage system T protein), len: 372 aa; similar to e.g. GCST_BACSU probable aminomethyltransferase (EC 2.1.2.10) (glycine cleavage system T protein) (362 aa), fasta scores; opt: 1074 z-score: 1108.6 E(): 0, 45.4% identity in 366 aa overlap 1 1 21 20 [Reporter]SCO1382 (3I20)_PCR/459 [Gene]SCO1382/417 NC_003888 SC1A8A.02c, conserved hypothetical protein, len: 157aa; similar to many eg. SW:Q50604 (Y0DE_MYCTU) hypothetical protein from Mycobacterium tuberculosis (164 aa) fasta scores; opt: 592, z-score: 709.0, E(): 4.6e-32, 60.2% identity in 161 aa overlap 1 2 15 20 [Reporter]SCO2225 (3A15)_PCR/2343 [Gene]SCO2225/2135 NC_003888 SC10B7.20c, possible isomerase, len: 119 aa; low similarities to SW:HPCD_ECOLI (EMBL:X53666) Escherichia coli 5-carboxymethyl-2-hydroxymuconate delta-isomerase HpcD, 125 aa; fasta scores: opt: 104 z-score: 146.1 E(): 0.93; 21.4% identity in 112 aa overlap 1 1 21 19 [Reporter]SCO5861 (4E20)_PCR/458 [Gene]SCO5861/416 NC_003888 SC2E9.02, unknown, len: 188 aa; not predicted by FramePlot 1 2 15 19 [Reporter]SCO7023 (4M11)_PCR/2342 [Gene]SCO7023/2134 NC_003888 SC1H10.12, unknown, len: 411 aa 1 1 21 18 [Reporter]SCO7106 (5A20)_PCR/457 [Gene]SCO7106/415 NC_003888 SC4B10.07, conserved hypothetical protein, len: 88 aa; similar to TR:Q9X952 (EMBL:AJ239087) Streptomyces coelicolor hypothetical 9.7 kDa protein WblE, 85 aa; fasta scores: opt: 382 z-score: 525.6 E(): 8.3e-22; 59.1% identity in 88 aa overlap and to TR:CAB93744 (EMBL:AL357613) Streptomyces coelicolor regulatory protein SC5F8.16c, 83 aa; fasta scores: opt: 368 z-score: 507.1 E(): 8.8e-21; 61.9% identity in 84 aa overlap 1 2 15 18 [Reporter]SCO2098 (5I11)_PCR/2341 [Gene]SCO2098/2133 NC_003888 SC4A10.31c, possible methyltransferase, len: 271 aa; shows weak similarity to SW:SMTA_ECOLI (EMBL:D26440), SmtA, Escherichia coli S-adenosylmethionine-dependent methyltransferase (261 aa), fasta scores; opt: 311 z-score: 330.3 E(): 4.5e-11, 32.0% identity in 250 aa overlap and to SC5F7.31 (EMBL:AL096872) S.coelicolor possible methyltransferase (233 aa) (34.3% identity in 236 aa overlap) 1 1 21 17 [Reporter]SCO2179 (6M16)_PCR/456 [Gene]SCO2179/414 NC_003888 SC5F7.22, probable aminopeptidase, len: 517aa; similar to SW:AMPA_ECOLI aminopeptidase from Escherichia coli (503 aa) fasta scores; opt: 844, z-score: 868.2, E(): 0, (34.7% identity in 522 aa overlap). Contains Pfam match to entry PF00883 Peptidase_M17, Cytosol aminopeptidase family and Prosite match to PS00631 Cytosol aminopeptidase signature. 1 2 15 16 [Reporter]SCO5692 (7A11)_PCR/2339 [Gene]SCO5692/2132 NC_003888 SC5H4.16c, probable transcriptional regulator, len: 337 aa; similar to SW:CELR_THEFU (EMBL:AF086819) Thermomonospora fusca transcription regulator CelR, 340 aa; fasta scores: opt: 440 z-score: 531.7 E(): 3.7e-22; 31.6% identity in 339 aa overlap. Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and match to Prosite entry PS00356 Bacterial regulatory proteins, lacI family signature. Contains also possible helix-turn-helix motif at residues 2..23 (+6.39 SD) 1 1 21 16 [Reporter]SCO2110 (7I16)_PCR/455 [Gene]SCO2110/413 NC_003888 SC6E10.04, pkaF, probable eukaryotic-type serine/threonine protein kinase, len: 667 aa; previously sequenced as TR:Q9ZNB3 (EMBL:AB019394) S.coelicolor eukaryotic-type protein kinase (629 aa) and identical to that sequence, but with a different putative start codon. Similar to many e.g. SW:PKN2_MYXXA (EMBL:M94857), Pkn2, Myxococcus xanthus serine/threonine protein kinase involved in regulation of beta-lactamase secretion (830 aa), fasta scores; opt: 564 z-score: 531.4 E(): 2.8e-22, 35.5% identity in 366 aa overlap. Similar to many hypothetical protein serine/threonine protein kinases from mycobacteria e.g. TR:O53510 (EMBL:AL021957) Mycobacterium tuberculosis (399 aa) (43.4% identity in 394 aa overlap). Similar to others from S.coelicolor e.g. SCH69.18 (EMBL:AL079308) S.coelicolor possible serine/threonine protein kinase (673 aa) (34.7% identity in 622 aa overlap). Alternative start codons are present at codons 3 and 11. Contains probable coiled-coil from 543 to 576 (34 residues) (Max score: 1.485, probability 0.93). Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and PS00108 Serine/Threonine protein kinases active-site signature and possible hydrophobic membrane spanning region 1 2 15 15 [Reporter]SCO2476 (8M7)_PCR/2338 [Gene]SCO2476/2131 NC_003888 SC7A8.15c, probable dehydrogenase/reductase (putative secreted protein), len: 247 aa; similar to TR:CAB52925 (EMBL:AL109949) Streptomyces coelicolor putative oxidoreductase SCJ11.40, 250 aa; fasta scores: opt: 591 z-score: 665.9 E(): 1.1e-29; 43.1% identity in 246 aa overlap and to SW:2BHD_STREX AC:P19992 Streptomyces exfoliatus (Streptomyces hydrogenans) 20-beta-hydroxysteroid dehydrogenase (EC 1.1.1.53), 25 aa; fasta scores: opt: 414 z-score: 469.7 E(): 9e-19; 36.1% identity in 249 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature. Contains also possible N-terminal region signal peptide sequence 1 1 21 15 [Reporter]SCO5239 (8E16)_PCR/454 [Gene]SCO5239/412 NC_003888 2SC7G11.01, possible histidine kinase, len: 491 aa; similar to TR:O05846 (EMBL:Z95120) Mycobacterium tuberculosis hypothetical 54.0 kDa protein MTCY07D11.06, 501 aa; fasta scores: opt: 1038 z-score: 1176.8 E(): 0; 48.9% identity in 380 aa overlap and to TR:O26226 (EMBL:AE000801) Methanobacterium thermoautotrophicum sensory transduction histidine kinase MTH123, 356 aa; fasta scores: opt: 372 z-score: 426.7 E(): 2.7e-16; 29.9% identity in 321 aa overlap. Contains Pfam match to entry PF00512 signal, Histidine kinase 1 2 15 14 [Reporter]SCO0004 (9I7)_PCR/2337 [Gene]SCO0004/2130 NC_003888 SC8E7.40c, unknown, len: 315 aa.,SC1C9.01, hypothetical protein (fragment), len: >64 aa; identical to C-terminal part of TR:Q9FBV5 (EMBL:AL391338) Streptomyces coelicolor hypothetical 33.7 kDa protein SC8E7.40c, 315 aa, situated on the opposite end of the chromosome 1 1 21 14 [Reporter]SCO7539 (9A16)_PCR/453 [Gene]SCO7539/411 NC_003888 SC8G12.15c, possible TetR-family transcriptional regulator, len: 244 aa. Similar to many e.g. Deinococcus radiodurans TR:AAF10298 (EMBL:AE001928) transcriptional regulator, TetR family (215 aa), fasta scores opt: 187 z-score: 223.2 E(): 5.6e-05 36.5% identity in 104 aa overlap. Also similar to many proteins of undefined function e.g. Mycobacterium tuberculosis SW:Y0DR_MYCTU (EMBL:Z78020) hypothetical 25.4 kd protein (234 aa), fasta scores opt: 372 z-score: 432.3 E(): 1.3e-16 34.1% identity in 229 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 42..63 (+5.38 SD). 1 1 21 13 [Reporter]SCO2590 (10M12)_PCR/452 [Gene]SCO2590/410 NC_003888 SCC88.01 possible glycosyltransferase, len: >1135 aa; N-terminal region similar to TR:Q9XC92 (EMBL:AF146532) Klebsiella pneumoniae glycosyltransferase, 329 aa; fasta scores: opt: 214 z-score: 245.4 E(): 3e-06; 21.3% identity in 263 aa overlap and C-terminal region similar to SW:TAGF_BACSU (EMBL:X15200) Bacillus subtilis teichoic acid biosynthesis protein F TagF, 746 aa; fasta scores: opt: 1056 z-score: 1203.0 E(): 0; 41.5% identity in 381 aa overlap,SCC123.28, possible glycosyl transferase, partial CDS, len: > 97 aa. Similar to several proteins associated with polysaccharide biosynthesis e.g. Lactococcus lactis TR:O06035(EMBL:AF036485) galactosyltransferase protein, EpsG (316 aa), fasta scores opt: 309 z-score: 414.0 E(): 1.1e-15 47.4% identity in 95 aa overlap and Streptococcus suis TR:Q9X4V4(EMBL:AF118389) putative glycosyltransferase, Cps2J (332 aa), fasta scores opt: 309 z-score: 413.6 E(): 1.2e-15 46.8% identity in 94 aa overlap. Also similar to the adjoining CDS, SCC123.27 (743 aa), fasta scores opt: 298 z-score: 353.4 E(): 2.9e-14 47.9% identity in 96 aa overlap. 2 3 9 15 [Reporter]SCO2153 (8F2)_PCR/4726 [Gene]SCO2153/4309 NC_003888 SC6G10.26c, possible secreted protein, len: 424aa; similar to many of undefined function eg. TR:O53223 (EMBL:AL021185) hypothetical protein from Mycobacterium tuberculosis (408 aa) fasta scores; opt: 867, z-score: 941.5, E(): 0, (35.2% identity in 403 aa overlap). Contains Prosite matches to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and PS00213 Lipocalin signature. Also contains possible N-terminal signal sequence. 2 3 9 14 [Reporter]SCO6604 (9B2)_PCR/4725 [Gene]SCO6604/4308 NC_003888 SC1F2.01, probable beta-glucosidase, partial CDS, len >311 aa; similar to mnay e.g. BGLS_AGRSP beta-glucosidase (EC 3.2.1.21) (459 aa), fasta scores; opt: 784 z-score: 986.7 E(): 0, 42.5% identity in 315 aa overlap. Overlaps and extends SC8A6.25. Contains Pfam match to entry PF00232 glycosyl_hydro1, Glycosyl hydrolases family 1, score 350.20, E-value 2.2e-101,SC8A6.25, probable beta-glucosidase, partial CDS, le n >190 aa; highly similar to many e.g. BGLA_BACCI beta-glucosidase (EC 3.2.1.21) (449 aa), fasta scores; opt: 752 z-sc ore: 1084.7 E(): 0, 59.9% identity in 167 aa overlap. Conta ins PS00653 Glycosyl hydrolases family 1 N-terminal signatu re and Pfam match to entry PF00232 glycosyl_hydro1, Glycosy l hydrolases family 1, score 348.50, E-value 7.3e-101 2 3 9 13 [Reporter]SCO1499 (9N22)_PCR/4724 [Gene]SCO1499/4307 NC_003888 SC9C5.23c, possible integral membrane protein, len: 582 aa. Contains possible hydrophobic membrane spanning region and degenerate repeat: 2x QDYQQQGY 2 3 9 12 [Reporter]SCO2761 (10J22)_PCR/4723 [Gene]SCO2761/4306 NC_003888 SCC57A.32c, possible secreted tripeptidyl aminopeptidase, len: 474 aa. Identical to Streptomyces lividans TR:Q54408(EMBL:L46588) tripeptidyl aminopeptidase precursor (474 aa), fasta scores opt: 3236 z-score: 3793.9 E(): 0; 99.6% identity in 474 aa overlap. Contains a possible N-terminal signal sequence. 2 3 9 11 [Reporter]SCO4090 (11F22)_PCR/4722 [Gene]SCO4090/4305 NC_003888 SCD25.26, unknown, len:283 aa. Previously sequenced Streptomyces coelicolor SW:YVDH_STRCO (EMBL; L13455) hypothetical 33.8 KD protein in VDH 3'region Orf1 (303 aa). 2 3 9 9 [Reporter]SCO0539 (13N18)_PCR/4720 [Gene]SCO0539/4304 NC_003888 SCF11.19, probable sugar transporter membrane protein, len: 316 aa; similar to TR:CAB52981 (EMBL:AL109950) Streptomyces coelicolor putative integral membrane permease, 319 aa; fasta scores: opt: 606 z-score: 689.2 E(): 5.1e-31; 34.9% identity in 312 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, binding-protein-dependent transport systems inner membrane component and a prosite match to entry PS00402 binding-protein-dependent transport systems inner membrane component signature. Contains probable N-terminal signal sequence 2 3 9 7 [Reporter]SCO1082 (15F18)_PCR/4718 [Gene]SCO1082/4303 NC_003888 SCG22.28c, probable electron transfer flavoprotein, beta subunit, len: 262 aa; similar to SW:ETFB_MYCTU (EMBL:AL021287) Mycobacterium tuberculosis putative electron transfer flavoprotein, beta subunit FixA or MTV012.44c, 266 aa; fasta scores: opt: 860 z-score: 939.3 E(): 0; 52.5% identity in 263 aa overlap. Contains Pfam match to entry PF01012 ETF_beta, Electron transfer flavoprotein beta subunit 1 2 15 13 [Reporter]SCO6217 (10E7)_PCR/2336 [Gene]SCO6217/2129 NC_003888 SC9G1.07c, unknown, len: 216 aa; similar to TR:CAB42748 (EMBL:AL049841) Streptomyces coelicolor hypothetical 40.6 KD protein SCE9.02c, 391 aa; fasta scores: opt: 158 z-score: 169.6 E(): 0.045; 28.8% identity in 198 aa overlap 2 3 9 6 [Reporter]SCO3426 (16B18)_PCR/4717 [Gene]SCO3426/4302 NC_003888 SCE9.33c, conserved hypothetical protein, len: 370 aa; similar to hypothetical proteins in Burkholderia, Mycobacterium tuberculosis and Bacillus subtilis. Similar to SW:P47K_PSECL (EMBL:D90216) Pseudomonas chlororaphis 47 kD protein critical for the expression of nitrile hydratase genes (419 aa), fasta scores; opt: 374 z-score: 409.4 E(): 1.8e-15, 28.1% identity in 392 aa overlap 1 2 15 12 [Reporter]SCO2535 (11A7)_PCR/2335 [Gene]SCO2535/2128 NC_003888 SCC77.02, unknown, len: 124 aa. Similar to several proteins of unknown function including: Streptomyces lavendulae TR:Q9X5T5(EMBL:AF127374) MmcQ, located within the antibiotic mitomycin C biosynthetic gene cluster (123 aa), fasta scores opt: 377 z-score: 487.1 E(): 9.6e-20 52.0% identity in 127 aa overlap and Deinococcus radiodurans TR:AAF11947(EMBL:AE002071) conserved hypothetical protein (132 aa), fasta scores opt: 299 z-score: 389.0 E(): 2.8e-14 43.0% identity in 128 aa overlap. 2 3 9 5 [Reporter]SCO0793 (17N14)_PCR/4716 [Gene]SCO0793/4301 NC_003888 SCF43.04c, unknown, len: 102 aa. 1 1 21 12 [Reporter]SCO4579 (11I12)_PCR/451 [Gene]SCO4579/409 NC_003888 SCD16A.04, possible integral membrane protein, len: 312aa; similar to others e.g. TR:O32910 (EMBL:AL008609) hypothetical protein from Mycobacterium leprae (316 aa) fasta scores; opt: 405, z-score: 470.7, E(): 6.9e-19, (32.7% identity in 324 aa overlap) and TR:O86628 (EMBL:AL031155) putative integral membrane protein from Streptomyces coelicolor (309 aa) fasta scores; opt: 222, z-score: 261.4, E(): 3.1e-07, (31.0% identity in 310 aa overlap). Contains possible membrane spanning hydrophobic regions 1 2 15 11 [Reporter]SCO4128 (12M3)_PCR/2334 [Gene]SCO4128/2127 NC_003888 SCD72A.14c, possible membrane protein, len: 513aa; similar to TR:Q9X871 (EMBL:AL049661) putative integral membrane protein from Streptomyces coelicolor (488 aa) fasta scores; opt: 220, z-score: 257.3, E(): 7.2e-07, 25.7% identity in 483 aa overlap. Contains possible membrane-spanning hydrophobic region. 2 3 9 4 [Reporter]SCO2235 (18J14)_PCR/4715 [Gene]SCO2235/4300 NC_003888 SCBAC17D6.02, conserved hypothetical protein, len: 87 aa; identical to previously sequenced SW:YU1E_STRCO (EMBL:Y17736) Streptomyces coelicolor hypothetical 9.7 kDa protein (ORFU1E), 87 aa and similar to SW:YEFM_ECOLI (EMBL:AE000293) Escherichia coli hypothetical 9.3 kDa protein in sbcB-hisL intergenic region, 83 aa; fasta scores: opt: 250 Z-score: 348.4 bits: 68.9 E(): 8e-12; 51.852% identity in 81 aa overlap. Contains Pfam match to entry PF02604 DUF172, Uncharacterized ACR, COG2161 1 1 21 11 [Reporter]SCO4482 (12E12)_PCR/450 [Gene]SCO4482/408 NC_003888 SCD69.02c, probable lysR-family transcriptional regulator, len: 286 aa; similar to TR:CAB55655 (EMBL:AL117385) Streptomyces coelicolor putative lysR-family transcriptional regulator SC5G9.07c, 287 aa; fasta scores: opt: 495 z-score: 551.1 E(): 3.1e-23; 37.0% identity in 297 aa overlap and to SW:ALSR_BACSU (EMBL:L04470) Bacillus subtilis als operon regulatory protein AlsR, 302 aa; fasta scores: opt: 390 z-score: 435.8 E(): 8.2e-17; 32.1% identity in 293 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Contains also possible helix-turn-helix motif at residues 19..42 (+4.15 SD) 1 2 15 10 [Reporter]SCO3039 (13I3)_PCR/2333 [Gene]SCO3039/2126 NC_003888 SCE34.20c, probable nucleotide phosphorylase, len: 360 aa; similar to SW:MPG1_YEAST (EMBL:U19608) Saccharomyces cerevisiae mannose-1-phosphate guanyltransferase (EC 2.7.7.13) Mpg1, 361 aa; fasta scores: opt: 570 z-score: 625.6 E(): 2.2e-27; 30.8% identity in 347 aa overlap. Contains Pfam matches to entries PF00483 NTP_transferase, Nucleotidyl transferase and 3x PF00132 hexapep, Bacterial transferase hexapeptide (four repeats) 1 1 21 9 [Reporter]SCO0914 (14M8)_PCR/448 [Gene]SCO0914/407 NC_003888 SCM10.02c, possible lipoprotein, len: 422 aa; similar to TR:CAB59588 (EMBL:AL132662) Streptomyces coelicolor putative sugar transporter sugar binding protein SCF11.11, 422 aa; fasta scores: opt: 1543 z-score: 1682.7 E(): 0; 57.6% identity in 427 aa overlap and to SW: MALE_ENTAE Enterobacter aerogenes maltose-binding periplasmic protein precursor MalE, 396 aa; fasta scores: opt: 224 z-score: 248.5 E(): 1.9e-06; 23.9% identity in 427 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and match correctly situated to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide sequence 1 2 15 9 [Reporter]SCO1582 (14E3)_PCR/2332 [Gene]SCO1582/2125 NC_003888 SCI35.04c, ATP/GTP binding protein, len: 174 aa; similar to TMRB_BACSU tunicamycin resistance protein (197 aa), fasta scores; opt: 282 z-score: 488.2 E(): 6.1e-20, 39.5% identity in 119 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 1 21 8 [Reporter]SCO1556 (15I8)_PCR/447 [Gene]SCO1556/406 NC_003888 SCL11.12c, possible acetyltransferase, len: 232 aa; similar to TR:CAB59477 (EMBL:AL132648) Streptomyces coelicolor putative acetyltransferase SCI41.07c, 196 aa; fasta scores: opt: 245 z-score: 310.5 E(): 7.1e-10; 33.7% identity in 175 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 2 15 8 [Reporter]SCO3778 (15A3)_PCR/2331 [Gene]SCO3778/2124 NC_003888 SCH63.25, possible threonyl tRNA synthetase, len: 430 aa; highly similar to C-terminal region of many threonyl tRNA synthetases, e.g. SW:SYT1_BACSU (EMBL:M36594) Bacillus subtilis threonyl tRNA synthetase 1 (EC 6.1.1.3) ThrS, 643 aa; fasta scores: opt: 1274 z-score: 1489.5 E(): 0; 50.9% identity in 379 aa overlap. Contains Pfam match to entry PF00587 tRNA-synt_2b, tRNA synthetase class II (G, H, P, S and T) 1 1 21 7 [Reporter]SCO3326 (16E8)_PCR/446 [Gene]SCO3326/405 NC_003888 SCE68.24c, possible epimerase, len: 353 aa; similar to many e.g. SW:GALE_SALT (EMBL:M33681), galE, Salmonella typhimurium UDP-glucose 4-epimerase (337 aa), fasta scores; opt: 209 z-score: 250.6 E(): 1.2e-06, 32.1% identity in 193 aa overlap. Similar to hypothetical proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. SW:YV28_MYCTU (EMBL:Z77162) Mycobacterium tuberculosis putative UDP-glucose 4-epimerase (376 aa) (45.0% identity in 353 aa overlap). N-terminal half weakly similar to the corresponding region of TR:O54156 (EMBL:AL021409), SC3F7.13, S.coelicolor probable oxidoreductase (347 aa) (29.8% identity in 181 aa overlap) 1 2 15 6 [Reporter]SCO3554 (16I23)_PCR/2329 [Gene]SCO3554/2123 NC_003888 SCH5.17c, possible integral membrane protein, len: 267aa; similar to TR:O69625 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (191 aa) fasta scores; opt: 324, z-score: 329.4, E(): 4.9e-11, (40.9% identity in 154 aa overlap). Contains possible membrane spanning regions. 1 1 21 6 [Reporter]SCO2871 (17A8)_PCR/445 [Gene]SCO2871/404 NC_003888 SCE6.08, unknown, len: 326 aa. Similar to Streptomyces coelicolor TR:CAB69667(EMBL:AL137165) hypothetical 34.9 kd protein, SCF42.05 (328 aa), fasta scores opt: 371 z-score: 450.8 E(): 1.1e-17 35.6% identity in 334 aa overlap. 1 2 15 4 [Reporter]SCO0066 (18A23)_PCR/2327 [Gene]SCO0066/2122 NC_003888 SCJ4.47, possible integral membrane permease, part of a possible binding protein dependent transport system, len: 319 aa; similar to many eg. SW:LACF_AGRRD lactose transport system permease from Agrobacterium radiobacter (298 aa) fasta scores; opt: 460, z-score: 522.1, E(): 1e-21, (30.5% identity in 272 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 1 1 21 5 [Reporter]SCO0125 (18M4)_PCR/444 [Gene]SCO0125/403 NC_003888 SCJ21.06, possible oxidoreductase, len: 527 aa; similar to hypothetical proteins associated with fatty acid or polyketide biosynthesis e.g. TR:O33907 (EMBL:U73935) hypothetical protein in eicosapentaenoic acid synthesis gene cluster from Shewanella sp. (543 aa), fasta scores; opt: 1474 z-score: 1595.4 E(): 0, 48.1% identity in 472 aa overlap; and TR:Q44210 (EMBL:U04436) ORF in region required for nitrogen fixation in Anabaena 7120 (442 aa), fasta scores; opt: 1395 z-score: 1511.4 E(): 0, 47.7% identity in 440 aa overlap. Also shows some similarity to predicted oxidoreductase from M. tuberculosis TR:O33323 oxidoreductase RV2781c (344 aa), fasta scores; opt: 161 z-score: 179.6 E(): 0.012, 26.7% identity in 277 aa overlap 1 2 15 3 [Reporter]SCO6304 (19M19)_PCR/2326 [Gene]SCO6304/2121 NC_003888 SCIF3.06c, probable oxidoreductase, len: 310 aa; similar to SW:MPC2_ALCEU (EMBL:X52415) Alcaligenes eutrophus metapyrocatechase 2 (EC 1.13.11.2) McpII, 320 aa; fasta scores: opt: 301 z-score: 359.5 E(): 1.7e-12; 28.1% identity in 299 aa overlap. Contains Pfam match to entry PF01013 Extradiol_dioxy, Extradiol ring-cleavage dioxygenase 1 1 21 4 [Reporter]SCP1.17 (19I4)_PCR/443 [Gene]SCP1.17/402 NC_003888 SCP1.17, unknown, len: 272aa; 1 2 15 2 [Reporter]SCO5998 (20E19)_PCR/2325 [Gene]SCO5998/2120 NC_003888 SCBAC1C11.01c, murA2, possible bifunctional protein (fragment), len: >138 aa; N-terminal domain similar to TR:Q9X153 (EMBL:AE001787) Thermotoga maritima lacI-family transcriptional regulator, putative TM1330, 111 aa; fasta scores: opt: 139 z-score: 193.0 E(): 0.0033; 39.4% identity in 66 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and possible helix-turn-helix motif at residues 22..43 (+7.45 SD) and C-terminal domain similar to TR:Q9PJT7 (EMBL:AE002342) Chlamydia muridarum UDP-N-acetylglucosamine 1-carboxyvinyltransferase TC0740, 442 aa; fasta scores: opt: 148 z-score: 195.2 E(): 0.0025; 38.1% identity in 63 aa overlap,SCBAC16H6.33c, murA2, possible UDP-N-acetylglucosamine transferase, len: 437 aa: similar to many eg. SW:P33986 (MURA_ACIGB) UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter genomosp. 11 (419 aa) fasta scores; opt: 438, Z-score: 509.1, 30.233% identity (31.863% ungapped) in 430 aa overlap and TR:Q9L1U5 (EMBL:AL138851) UDP-N-acetylglucosamine transferase from Streptomyces coelicolor SCE59.08 (448 aa) fasta scores; opt: 484, Z-score: 561.7, 29.890% identity (33.252% ungapped) in 455 aa overlap. Contains Pfam match to entry PF00275 EPSP_syntase, EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase). 1 1 21 3 [Reporter]SCO7524 (20E4)_PCR/442 [Gene]SCO7524/401 NC_003888 SCBAC25F8.16, conserved hypothetical protein, len: 172 aa; similar to SW:YMEL_STRLN (EMBL:X95703) Streptomyces lincolnensis hypothetical 17.2 kDa protein in melC2-rnhH intergenic region, 150 aa; fasta scores: opt: 420 Z-score: 503.1 bits: 99.3 E(): 2.1e-20; 40.268% identity in 149 aa overlap 1 1 21 2 [Reporter]SCO3974 (20M24)_PCR/441 [Gene]SCO3974/400 NC_003888 SCBAC25E3.11c, unknown, len: 321aa: contains Pfam match to entry PF02810 SEC-C, SEC-C motif. 1 2 14 22 [Reporter]SCO1001 (1E15)_PCR/2323 [Gene]SCO1001/2119 NC_003888 2SCG2.14, unknown, len: 120 aa 1 2 14 21 [Reporter]SCO3138 (2A15)_PCR/2322 [Gene]SCO3138/2118 NC_003888 SCE66.17c, galT, galactose-1-phosphate uridylyltransferase (EC 2.7.7.10), len: 353aa; similar to many especially SW:P13212 (GAL7_STRLI) galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa) fasta scores; opt: 1998, z-score: 2363.0, E(): 0, 87.2% identity in 352 aa overlap. Contains Pfam match to entry PF01087 GalP_UDP_transf, Galactose-1-phosphate uridyl transferase and Prosite match to PS00117 Galactose-1-phosphate uridyl transferase family 1 active site signature. 1 2 14 20 [Reporter]SCO5916 (3M11)_PCR/2321 [Gene]SCO5916/2117 NC_003888 SC10A5.21, unknown, len: 170 aa; contains probable helix-turn-helix motif at aa 90 to 111 (Score 1413, +4.00 SD) 1 2 14 19 [Reporter]SCO7017 (4I11)_PCR/2320 [Gene]SCO7017/2116 NC_003888 SC1H10.06c, possible membrane protein, len: 297 aa. Similar to several including: Streptomyces coelicolor TR:Q9XAL0 (EMBL:AL079348) putative membrane protein, SC66T3.03 (330 aa), fasta scores opt: 561 z-score: 634.9 E(): 6.4e-28 50.8% identity in 315 aa overlap. Contains possible membrane spanning hydrophobic domains 1 2 14 18 [Reporter]SCO5453 (5E11)_PCR/2319 [Gene]SCO5453/2115 NC_003888 SC3D11.10, possible integral membrane protein, len: 249aa; similar to many proposed membrane proteins eg. TR:Q9XAC7 (EMBL:AL096839) putative integral membrane transport protein from Streptomyces coelicolor (269 aa) fasta scores; opt: 292, z-score: 325.6, E(): 1e-10, 29.8% identity in 245 aa overlap. Contains possible membrane-spanning hydrophobic regions. 1 2 14 17 [Reporter]SCO5795 (6A11)_PCR/2318 [Gene]SCO5795/2114 NC_003888 SC4H2.16, probable zinc metalloprotease (putative membrane protein), len: 512 aa; weakly similar to the N-terminus of many, longer, aminopeptidases, e.g. AMPN_LACLA Q48656 aminopeptidase n (ec 3.4.11.2) (848 aa), fasta scores; opt: 304 z-score: 287.5 E(): 8.6e-09, 25.2% identity in 440 aa overlap. Contains match to Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature and Pfam match to entry zn-protease PF00099, Zinc-binding metalloprotease domain, score 28.22. Contains possible hydrophobic membrane spanning region 1 2 14 16 [Reporter]SCO6762 (7M7)_PCR/2317 [Gene]SCO6762/2113 NC_003888 SC6A5.11, possible phytoene dehydrogenase, len: 478aa; similar to many from eukaryotes eg. SW:CRTI_CAPAN phytoene dehydrogenase precursor from Capsicum annuum (bell pepper) (582 aa) fasta scores; opt: 276, z-score: 302.1, E(): 1.6e-09, (24.0% identity in 459 aa overlap). Also similar to prokaryotes eg. TR:P72449 (EMBL:X95596) proposed dehydrogenase from a cryptic carotenoid biosynthesis cluster in Streptomyces griseus (517 aa) fasta scores; opt: 206, z-score: 226.8, E(): 2.5e-05, (29.7% identity in 512 aa overlap) 1 2 14 15 [Reporter]SCO2472 (8I7)_PCR/2316 [Gene]SCO2472/2112 NC_003888 SC7A8.11, possible integral membrane protein, len: 228 aa; similar to SW:YGJQ_ECOLI (EMBL:U18997) Escherichia coli hypothetical 25,5 kD protein, 230 aa; fasta scores: opt: 405 z-score: 493.9 E(): 4e-20; 37.7% identity in 207 aa overlap and to SW:SANA_ECOLI (EMBL:Z47804) Escherichia coli SanA protein, 239 aa; fasta scores: opt: 328 z-score: 401.5 E(): 5.7e-15; 34.7% identity in 170 aa overlap. Contains possible N-terminal region signal peptide sequence 1 2 14 14 [Reporter]SCO7741 (9E7)_PCR/2315 [Gene]SCO7741/2111 NC_003888 SC8D11.32c, possible bifunctional protein, len: 354 aa; N-terminal region similar to TR:Q9RKU1 (EMBL:AL132824) Streptomyces coelicolor putative reductase SCAH10.16, 185 aa; fasta scores: opt: 387 z-score: 473.3 E(): 8e-19; 39.0% identity in 187 aa overlap and C-terminal region similar to SW:YIEF_ECOLI (EMBL:L10328) Escherichia coli hypothetical 20.4 kDa protein YieF, 188 aa; blastp scores: Score= 148 (52.1 bits), Expect= 7.0e-10, P= 7.0e-10; Identities= 49/139 (35%), Positives= 78/139 (56%). Contains Pfam match in C-terminal region to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 2 14 13 [Reporter]SCO6213 (10A7)_PCR/2314 [Gene]SCO6213/2110 NC_003888 SC9G1.03, probable hydrolase, len: 465 aa; similar to SW:ATZB_PSESD (EMBL:U66917) Pseudomonas sp. (strain ADP) hydroxyatrazine hydrolase (EC 3.-.-.-) AtzB, 481 aa; fasta scores: opt: 794 z-score: 921.6 E(): 0; 36.8% identity in 457 aa overlap. Contains match to Pfam entry PF01685 ATZ_TRZ 1 2 14 12 [Reporter]SCO2365 (11M3)_PCR/2313 [Gene]SCO2365/2109 NC_003888 SCC8A.23c, unknown, len: 245 aa; similar to proteins associated with resistance to tellurium salts eg. TR:Q44313 (EMBL:M20238) tellurium salts resistance protein from plasmid pMER610 of Alcaligenes sp. (339 aa) fasta scores; opt: 408, z-score: 510.3, E(): 5.8e-21, 38.4% identity in 219 aa overlap 1 2 14 11 [Reporter]SCO4124 (12I3)_PCR/2312 [Gene]SCO4124/2108 NC_003888 SCD72A.10c, possible two-component sensor kinase, len: 456aa; similar to many eg. TR:O86631 (EMBL:AL031155) putative two-component sensor kinase from Streptomyces coelicolor (429 aa) fasta scores; opt: 929, z-score: 995.1, E(): 0, 42.7% identity in 422 aa overlap. Contains Pfam match to entry PF00512 signal, Histidine kinase and possible membrane-spanning hydrophobic regions. Contains possible hydrophobic membrane spanning regions 1 2 14 10 [Reporter]SCO3192 (13E3)_PCR/2311 [Gene]SCO3192/2107 NC_003888 SCE22.09, possible integral membrane protein, len: 440 aa; similar to TR:AAF10839 (EMBL:AE001974) Deinococcus radiodurans conserved hypothetical protein DR1273, 429 aa; fasta scores: opt: 365 z-score: 385.0 E(): 5.5e-14; 28.6% identity in 413 aa overlap. High content in alanine amino acid residues. Contains possible hydrophobic membrane spanning regions 1 2 14 7 [Reporter]SCO0645 (15I23)_PCR/2308 [Gene]SCO0645/2106 NC_003888 SCF91.05c, possible Zinc-containing dehydrogenase, len: 337 aa. Similar to many dehydrogenases including: Alcaligenes eutrophus SW:ADH_ALCEU(EMBL:J03362) alcohol dehydrogenase (EC 1.1.1.1) (366 aa), fasta scores opt: 272 z-score: 296.1 E(): 4e-09 27.0% identity in 371 aa overlap and Streptomyces coelicolor TR:Q53927(EMBL:X62373) hydroxyacyl-CoA dehydrogenase (329 aa), fasta scores opt: 308 z-score: 335.0 E(): 2.8e-11 31.8% identity in 333 aa overlap. Contains a Prosite hit to PS00059 Zinc-containing alcohol dehydrogenases signature and a Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases. 1 2 14 6 [Reporter]SCO3922 (16E23)_PCR/2307 [Gene]SCO3922/2105 NC_003888 SCQ11.05c, possible membrane protein, len: 125 aa; previously sequenced as SW:YPT3_STRCO (EMBL:U37580) hypothetical 15.9 kD protein in ptpA 5' region (148 aa), with different putative start codon. Start codon position is suggested here by GC frameplot analysis. Similar to others from mycobacteria and Bacillus subtilis e.g. TR:O07729 (EMBL:Z97193) Mycobacterium tuberculosis possible membrane protein (134 aa) (42.9% identity in 126 aa overlap) 1 2 14 5 [Reporter]SCO1171 (17A23)_PCR/2306 [Gene]SCO1171/2104 NC_003888 SCG11A.02, possible xylose repressor, len: 402 aa; similar to SW:XYLR_BACSU (EMBL:M27248) Bacillus subtilis xylose repressor xylR, 384 aa; fasta scores: opt: 612 z-score: 642.4 E(): 2.1e-28; 29.4% identity in 381 aa overlap. Contains match to Pfam entry PF00480 ROK, ROK family and a possible helix-turn-helix motif at residues 43..64 (+3.15 SD) 1 2 14 4 [Reporter]SCO4899 (18M19)_PCR/2305 [Gene]SCO4899/2103 NC_003888 2SCK8.25, hypothetical protein, len: 292 aa; similar to TR:Q9X7Q1 (EMBL:AL049587) Streptomyces coelicolor hypothetical alanine-rich protein SC5F2A.28c, 292 aa; fasta scores: opt: 716 z-score: 814.7 E(): 0; 44.9% identity in 274 aa overlap 1 2 14 3 [Reporter]SCO4996 (19I19)_PCR/2304 [Gene]SCO4996/2102 NC_003888 2SCK36.19c, probable RNA polymerase ECF sigma factor, len: 169 aa; similar to SW:RPOE_STRCO (EMBL:L29636) Streptomyces coelicolor RNA polymerase sigma-E factor SigE or SCE94.07, 176 aa; fasta scores: opt: 278 Z-score: 347.2 bits: 70.7 E(): 9.9e-12; 36.527% identity in 167 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 1 2 14 2 [Reporter]SCO3981 (20A19)_PCR/2303 [Gene]SCO3981/2101 NC_003888 SCBAC25E3.18c, possible GntR-family transcriptional regulator, len: 251aa: similar to many eg. TR:Q07191 (EMBL:Z19589) KorSA regulatory protein from Streptomyces ambofaciens plasmid pSAM2 (259 aa) fasta scores; opt: 323, Z-score: 366.6, 30.952% identity (32.911% ungapped) in 252 aa overlap. Also strongly similar to TR:Q9X8E2 (EMBL:AL049573) putative GntR-family regulator SCE39.19 from Streptomyces coelicolor (251 aa) fasta scores; opt: 1603, Z-score: 1776.4, 96.386% identity (96.386% ungapped) in 249 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. 1 2 13 22 [Reporter]SCO0993 (1A15)_PCR/2301 [Gene]SCO0993/2100 NC_003888 2SCG2.06, conserved hypothetical protein, len: 412 aa; similar to SW:Y4RH_RHISN (EMBL:AE000094) Rhizobium sp. hypothetical 52.5 kDa protein Y4rH, 478 aa; fasta scores: opt: 348 z-score: 384.2 E(): 7.1e-14; 26.3% identity in 400 aa overlap 1 3 12 11 [Reporter]SCO4453 (12A2)_PCR/4284 [Gene]SCO4453/3899 NC_003888 SCD6.31c, hypothetical protein, len: 135 aa; similar to TR:O86797 (EMBL:AL031317) Streptomyces coelicolor hypothetical 13.2 kD protein, 118 aa; fasta scores: opt: 158 z-score: 203.7 E(): 0.00069; 34.0% identity in 103 aa overlap 1 3 12 10 [Reporter]SCO2828 (12M22)_PCR/4283 [Gene]SCO2828/3898 NC_003888 SCE20.02, probable amino acid ABC transporter protein, solute-binding component, len: 309 aa. Similar to several including: Salmonella typhimurium SW:ARGT_SALTY(EMBL:V01368) lysine-arginine-ornithine-binding periplasmic protein precursor (260 aa), fasta scores opt: 251 z-score: 294.5 E(): 5.1e-09 25.8% identity in 279 aa overlap and Rhizobium sp. (strain NGR234) SW:Y4TE_RHISN(EMBL:AE000098) probable amino-acid ABC transporter periplasmic binding protein Y4TE (300 aa), fasta scores opt: 599 z-score: 691.7 E(): 3.8e-31 35.4% identity in 297 aa overlap. Contains a Pfam match to entry PF00497 SBP_bac_3, Bacterial extracellular solute-binding proteins, family 3. Also contains an N-terminal possible membrane spanning hydrophobic domain. 1 3 12 9 [Reporter]SCO1775 (13I22)_PCR/4282 [Gene]SCO1775/3897 NC_003888 SCI51.15c, conserved hypothetical protein, len: 211 aa; unknown function, similar to many bacterial hypothetical proteins e.g. TR:O33199 (EMBL:Z98268) Mycobacterium tuberculosis hypothetical protein (207 aa), fasta scores; opt: 508 z-score: 616.4 E(): 5.5e-27, 43.1% identity in 197 aa overlap. Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein 1 3 12 8 [Reporter]SCO5035 (14E22)_PCR/4281 [Gene]SCO5035/3896 NC_003888 SCK7.08c, possible ABC transporter ATP-binding protein, len: 229 aa; similar to TR:CAB81857 (EMBL:AL161691) Streptomyces coelicolor putative ABC transporter ATP-binding protein SCD40A.12c, 246 aa; fasta scores: opt: 597 z-score: 668.0 E(): 9.7e-30; 47.6% identity in 206 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 12 7 [Reporter]SCO3861 (15A22)_PCR/4280 [Gene]SCO3861/3895 NC_003888 SCH69.31c, putative membrane protein, len: 441 aa. Contains possible hydrophobic membrane spanning regions 1 3 12 6 [Reporter]SCO3754 (16M18)_PCR/4279 [Gene]SCO3754/3894 NC_003888 SCH63.01c, possible ABC transport system integral membrane protein (fragment), len: >319 aa; similar to TR:CAB77000 (EMBL:AL159178) Streptomyces coelicolor putative integral membrane protein SCH22A.31, 850 aa; fasta scores: opt: 510 z-score: 493.4 E(): 5.8e-20; 38.1% identity in 315 aa overlap. Contains possible hydrophobic membrane spanning regions,SCH22A.32c, possible transmembrane transport protein (fragment), len: >569 aa; similar to TR:Q9XAA6 (EMBL:AL096837) Streptomyces coelicolor putative transmembrane transport protein SCF43A.09 463 a; fasta scores: opt: 255 z-score: 268.0 E(): 1.6e-07; 30.4% identity in 415 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 12 5 [Reporter]SCO0278 (17I18)_PCR/4278 [Gene]SCO0278/3893 NC_003888 SCF85.06, MutT domain containing protein, len: 204. Similar to the MutT domains of other proteins e.g. Bacillus subtilis SW:YZGD_BACSU (EMBL:U17168) hypothetical 45.4 KD protein in thiaminase (413 aa), fasta scores opt: 138 z-score: 176.6 E(): 0.017 36.1% identity in 97 aa overlap. Contains a Pfam match to entry PF00293 mutT, Bacterial MutT protein. 1 3 12 4 [Reporter]SCO0471 (18E18)_PCR/4277 [Gene]SCO0471/3892 NC_003888 SCF76.11, possible araC family transcriptional regulator, len: 326 aa. Weakly similar to the DNA-binding domains of other transcriptional regulators e.g. Escherichia coli SW:ARAC_ECOLI (EMBL; V00256) arabinose operon regulatory protein (292 aa), fasta scores opt: 130 z-score: 151.4 E(): 0.45 28.2% identity in 163 aa overlap. Contains a Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. The putative helix-turn-helix motif is located between residues 197..218 (+2.74 SD). Also contains a possible membrane spanning hydrophobic domain. 1 3 12 3 [Reporter]SCO4877 (19A18)_PCR/4276 [Gene]SCO4877/3891 NC_003888 2SCK8.03c, unknown, len: 444 aa 1 3 12 2 [Reporter]SCO5973 (20I14)_PCR/4275 [Gene]SCO5973/3890 NC_003888 SCBAC16H6.08, possible phosphatase, len: 842 aa: similar to TR:O69213 (EMBL:AJ224354) protein serine-threonine phosphatase from Anabaena sp. (strain PCC 7120) fasta scores; opt: 2173, Z-score: 2321.6, 52.962% identity (54.808% ungapped) in 861 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 11 22 [Reporter]SCO1252 (1I10)_PCR/4273 [Gene]SCO1252/3889 NC_003888 2SCG1.27, possible integral membrane protein, len: 337 aa; similar to TR:Q9RKZ3 (EMBL:AL133213) Streptomyces coelicolor putative integral membrane protein SC6D7.04, 347 aa; fasta scores: opt: 1101 z-score: 1230.5 E(): 0; 50.7% identity in 337 aa overlap. Contains Pfam matches to entries PF01595 DUF21, Domain of unknown function DUF21 and 2x PF00571 CBS, CBS domain. Also contains possible N-terminal region signal peptide sequence (seems to be cleavable) and possible hydrophobic membrane spanning region 1 3 11 21 [Reporter]SCO1648 (2E10)_PCR/4272 [Gene]SCO1648/3888 NC_003888 SCI41.31c, arc AAA ATPase, len: 588 aa. Identical to TR:O87594 (EMBL:AF086832) Streptomyces coelicolor ARC, AAA ATPase forming ring-shaped complexes and similar to TR:O50202 (EMBL:AF088800) Rhodococcus erythropolis ARC protein, 591 aa; fasta scores: opt: 2036 z-score: 2155.8 E():0; 67.1% identity in 587 aa overlap. Pfam match to entry PF00004 AAA, ATPases associated with various cellular activities (AAA). Contains probable coiled-coil from 47 to 93 (47 residues, max score: 1.592, probability 0.99) 1 3 11 20 [Reporter]SCO4086 (3A10)_PCR/4271 [Gene]SCO4086/3887 NC_003888 SCD25.22, purF, amidophosphoribosyltransferase, len: 530 aa. Identical to Streptomyces lividans TR:Q9ZB04 (EMBL; U64826) glutamine phosphoribosylpyrophosphate amidotransferase (EC 2.4.2.14) (530 aa), fasta scores opt: 3480 z-score: 3887.1 E(): 0 99.8% identity in 530 aa overlap and highly similar to Synechococcus sp. (strain PCC 7942) SW:PUR1_SYNP7 (EMBL; U33211) amidophosphoribosyltransferase precursor (EC 2.4.2.14) (glutamine phosphoribosylpyrophosphate amidotransferase) (ATase) (GPATase) (493 aa), fasta scores opt: 1630 z-score: 1822.2 E(): 0 52.5% identity in 495 aa overlap. Contains 2x Pfam match to entry PF00310 GATase_2, Glutamine amidotransferases class-II and a Prosite hit to PS00103 Purine/pyrimidine phosphoribosyl transferases signature. 1 3 11 19 [Reporter]SCO6626 (4M6)_PCR/4270 [Gene]SCO6626/3886 NC_003888 SC1F2.23, unknown, len: 1557 aa; some simlarity to several protein kinases e.g. ABL_MLVAB tyrosine-protein kinase transforming protein from Abelson murine leukemia virus (746 aa), fasta scores; opt: 84 z-score: 234.0 E(): 8.6e-06, 24.7% identity in 364 aa overlap. Contains PS00107 Protein kinases ATP-binding region signature, Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain, score 18.10, E-value 0.00062 and possible helix-turn-helix motif at aa 1027-1048 (Score 1753, +5.16 SD) 1 3 11 18 [Reporter]SCO6580 (5I6)_PCR/4269 [Gene]SCO6580/3885 NC_003888 SC8A6.01, unknown, partial CDS, len: >215 aa; overlaps and extends SC3F9.15,SC3F9.15, unknown, partial CDS len: >224 aa 1 3 11 17 [Reporter]SCO2044 (6E6)_PCR/4268 [Gene]SCO2044/3884 NC_003888 SC4G6.13c, possible phosphoribosyl-AMP cyclohydrolase, len: 128aa; similar to but shorter than many eg. SW:HIS2_ECOLI phosphoribosyl-AMP cyclohydrolase from Escherichia coli (203 aa) fasta scores; opt: 343, z-score: 429.1, E(): 1.4e-16, (52.7% identity in 93 aa overlap). Also similar to SW:HIS3_MYCTU phosphoribosyl-AMP cyclohydrolase from Mycobacterium tuberculosis (115 aa) fasta scores; opt: 471, z-score: 588.3, E(): 1.9e-25, (64.2% identity in 109 aa overlap). 1 3 11 16 [Reporter]SCO7277 (7A6)_PCR/4267 [Gene]SCO7277/3883 NC_003888 SC5H1.15c, possible regulator protein, len: 151 aa; similar to Streptomyces fradiae beta-lactamase regulator protein (EMBL:AB024056) (139 aa), fasta scores; opt: 177 z-score: 241.8 E(): 3.8e-06, 35.2% identity in 122 aa overlap and TR:O54111 (EMBL:AL021529), SC10A5.20, S.coelicolor hypothetical protein (171 aa) (35.4% identity in 99 aa overlap) 1 3 11 15 [Reporter]SCO5570 (8M2)_PCR/4266 [Gene]SCO5570/3882 NC_003888 SC7A1.14, unknown, len: 217 aa; similar to Y06F_MYCTU hypothetical protein from Mycobacterium tuberculosis (207 aa) fasta scores; opt: 244, z-score: 699.9, E(): 1.1e-31, (45.5% identity in 189 aa overlap). 1 3 11 14 [Reporter]SCO2648 (9I2)_PCR/4265 [Gene]SCO2648/3881 NC_003888 SC8E4A.18c, possible membrane protein, len: 95 aa. Contains possible hydrophobic membrane spanning regions 1 3 11 13 [Reporter]SCO6486 (10E2)_PCR/4264 [Gene]SCO6486/3880 NC_003888 SC9C7.22, possible transport associated protein, len: 277aa; similar to SW:DPPA_BACSU dppA, dipeptide transport protein (274 aa) fasta scores; opt: 448, z-score: 604.3, E(): 2.3e-26, (32.3% identity in 269 aa overlap). 1 3 11 12 [Reporter]SCO2710 (11A2)_PCR/4263 [Gene]SCO2710/3879 NC_003888 SCC61A.31, possible polysaccharide deacetylase, len: 229 aa; weakly similar to many e.g. TR:Q9XDJ5 (EMBL:AF048749) putative deacetylase from Bacteroides fragilis (259 aa) fasta scores; opt: 135, z-score: 169.7, E(): 0.054, 22.6% identity in 137 aa overlap. Contains Pfam match to entry PF01522 Polysac_deacet, Polysaccharide deacetylase. 1 3 11 11 [Reporter]SCO4263 (11M22)_PCR/4262 [Gene]SCO4263/3878 NC_003888 SCD49.04, possible transcriptional regulatory protein, len: 1251 aa; N-terminal domain similar to TR:Q9RD3 (EMBL:AL133422) Streptomyces coelicolor putative transcriptional regulatory protein SCM1.10, 888 aa; blastp scores: Score = 154 (54.2 bits), Expect = 5.7e-15, Sum P(2) = 5.7e-15 Identities = 120/425 (28%), Positives = 176/425 (41%) and C-terminal domain similar to TR:Q9ZGI0 (EMBL:AF079139) Streptomyces venezuelae putative transcriptional activator PkiD, 928 aa; fasta scores: opt: 602 z-score: 498.1 E(): 2.9e-20; 29.0% identity in 815 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Contains a helix-turn-helix motif at residues 1196..1217 (+3.89 SD), also a TTA leucine codon, possible target for bldA regulation and 4x degenerate repeat unit: (A/S)(A/S)AP(G/D)AAYXQAP(P/S)SDG(A/V)Y 1 3 11 10 [Reporter]SCO3534 (12I22)_PCR/4261 [Gene]SCO3534/3877 NC_003888 SCE2.15, possible large ATP-binding protein, len: 840aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) and a probable coiled-coil region around 600-650aa. 1 3 11 9 [Reporter]SCO1636 (13E22)_PCR/4260 [Gene]SCO1636/3876 NC_003888 SCI41.19c, unknown, len: 360 aa. Similar to various hypothetical proteins, e.g. TR:P72265 (EMBL:Z82004) Rhodococcus erythropolis ORF11, 326 aa; fasta scores: opt: 694 z-score: 768.9 E(): 0; 39.8% identity in 322 aa overlap 1 3 11 8 [Reporter]SCO0552 (14A22)_PCR/4259 [Gene]SCO0552/3875 NC_003888 SCF11.32c, possible response regulator, len: 238 aa; similar to TR:CAB54568 (EMBL:AJ006392) Streptococcus pneumoniae response regulator Rro2, 234 aa; fasta scores: opt: 583 z-score: 693.9 E(): 2.8e-31; 43.2% identity in 227 aa overlap. Contains Pfam matches to entries PF00486 trans_reg_C, transcriptional regulatory protein and PF00072 response_reg, response regulator receiver domain 1 3 11 7 [Reporter]SCO0222 (15M18)_PCR/4258 [Gene]SCO0222/3874 NC_003888 SCJ9A.01, possible acetyltransferase, len: 177 aa; similar to many e.g. TR:Q53796 (EMBL:L26955) bleomycin acetyltransferase from Streptomyces verticillus (301 aa) fasta scores; opt: 150, z-score: 190.2, E(): 0.0031, (30.8% identity in 169 aa overlap). Also similar to TR:CAB44535 (EMBL:AL078618) putative acetyltransferase from Streptomyces coelicolor (187 aa) fasta scores; opt: 215, z-score: 271.9, E(): 8.8e-08, (34.2% identity in 146 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 3 11 6 [Reporter]SCO2899 (16I18)_PCR/4257 [Gene]SCO2899/3873 NC_003888 SCE6.36c, possible GroES-family molecular chaperone, len: 110 aa. Similar to many members of the GroES chaperonin family including: Rhizobium meliloti SW:CH13_RHIME(EMBL:M94191) 10 KD chaperonin C (GroES-C) (101 aa), fasta scores opt: 172 z-score: 231.3 E(): 1.9e-05 33.7% identity in 101 aa overlap. Contains 2xPfam matches to entry PF00166 cpn10, Chaperonins 10 Kd subunit. 4 1 16 18 [Reporter]SCO6373 (5P12)_PCR/1859 [Gene]SCO6373/1699 NC_003888 SC4A2.09, probable integral membrane protein, len: 465 aa. Contains possible hydrophobic membrane spanning regions 1 3 11 5 [Reporter]SCO3750 (17E18)_PCR/4256 [Gene]SCO3750/3872 NC_003888 SCH22A.28c, possible two-component sensor histidine kinase, len: 416 aa; similar to SW:UHPB_SALTY (EMBL:M89480) Salmonella typhimurium sensor protein UhpB (EC 2.7.3.-), 500 aa; fasta scores: opt: 274 z-score: 299.1 E(): 3e-09; 26.4% identity in 337 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 16 16 [Reporter]SCO3593 (7H12)_PCR/1857 [Gene]SCO3593/1698 NC_003888 SC66T3.04, hypothetical protein, len: 397 aa; unknown function, weakly similar to many femA proteins e.g. SW:FEMA_STAAU (EMBL:M23918), FemA, Staphylococcus aureus factor essential for expression of methicillin resistance (433 aa), fasta scores; opt: 184 z-score: 218.4 E(): 7.7e-05, 23.3% identity in 434 aa overlap. Similar to SCH24.26c (EMBL:AL049826) S.coelicolor hypothetical protein (373 aa) (50.4% identity in 387 aa overlap) 1 3 11 4 [Reporter]SCO1512 (18A18)_PCR/4255 [Gene]SCO1512/3871 NC_003888 SCL2.02, unknown, len: 46 aa 4 1 16 15 [Reporter]SCO5292 (8D12)_PCR/1856 [Gene]SCO5292/1697 NC_003888 SC6G9.41c, cvnD5, possible ATP/GTP-binding protein, len: 191 aa; highly similar to others from S.coelicolor e.g. TR:O86519 (EMBL:AL031124) S.coelicolor possible ATP/GTP-binding protein (174 aa), fasta scores; opt: 766 z-score: 918.0 E(): 0, 68.0% identity in 169 aa overlap. Although many of the presumed homologues start at the equivalent of codon 18 in this CDS, there is no possible initiation codon in this case. Also similar to hypothetical proteins from other organisms e.g. TR:O5039 (EMBL:AL009198) Mycobacterium tuberculosis hypothetical protein (193 aa) (63.6% identity in 173 aa overlap). Similar to the C-terminal half of TR:O26694 (EMBL:AE00084) Methanobacterium thermoautotrophicum conserved protein (393 aa) (29.5% identity in 173 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 11 3 [Reporter]SCO4880 (19M14)_PCR/4254 [Gene]SCO4880/3870 NC_003888 2SCK8.06, possible transferase, len: 424 aa; similar to TR:O88719 (EMBL:AJ006215) Mus musculus CMP-N-acetylneuraminic acid synthetase (EC 2.7.7.43) CmaS, 432 aa; fasta scores: opt: 361 z-score: 400.1 E(): 1e-14; 30.3% identity in 389 aa overlap. Contains Pfam matches to entries PF02348 Cytidylyl_trans, Cytidylyl transferase and PF00702 Hydrolase, haloacid dehalogenase-like hydrolase 4 1 16 14 [Reporter]SCO6988 (9P8)_PCR/1855 [Gene]SCO6988/1696 NC_003888 SC8F11.14c, possible oxidoreductase, len: 387 aa. Similar to several including: Sinorhizobium meliloti TR:O68965(EMBL:) myo-inositol dehydrogenase (EC 1.1.1.18) IdhA (330 aa), fasta scores opt: 287 z-score: 332.8 E(): 4.2e-11 30.1% identity in 356 aa overlap and Streptomyces coelicolor TR:O69945(EMBL:AL023862) putative oxidoreductase, SC3F9.05 (430 aa), fasta scores opt: 721 z-score: 825.3 E():0 34.6% identity in 390 aa overlap. Contains a Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family. 4 1 16 13 [Reporter]SCO2517 (10L8)_PCR/1854 [Gene]SCO2517/1695 NC_003888 SCC121.20c, possible two-component system response regulator, len: 227 aa; similar to various S. coelicolor two-component response regulators, e.g. TR:CAB51249 (EMBL:AL096872) Streptomyces coelicolor putative two-component response regulator SC5F7.36c, 227 aa; fasta scores: opt: 752 z-score: 883.6 E(): 0; 57.7% identity in 220 aa overlap. Contains two Pfam matches to entries PF00072 response_reg and PF00196 GerE. Contains also a possible helix-turn-helix motif at residues 168..189 (+3.69 SD) 4 1 16 12 [Reporter]SCO2550 (11H8)_PCR/1853 [Gene]SCO2550/1694 NC_003888 SCC77.17c, possible lipoprotein, len: 171 aa. Contains an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. 4 1 16 11 [Reporter]SCO4467 (12D8)_PCR/1852 [Gene]SCO4467/1693 NC_003888 SCD65.10c, unknown, len: 145 aa 4 1 16 10 [Reporter]SCO3443 (13P4)_PCR/1851 [Gene]SCO3443/1692 NC_003888 SCE36.10c, probable pyridine nucleotide-disulphide oxidoreductase, len: 454 aa; similar to many eg. SW:MERA_STRLI mercuric reductase from Streptomyces lividans (474 aa) fasta scores; opt: 752, z-score: 772.0, E(): 0, (34.5% identity in 464 aa overlap). Contains Pfam match to entry PF00070 pyr_redox, Pyridine nucleotide-disulphide oxidoreductase class-I. 4 1 16 9 [Reporter]SCO1728 (14L4)_PCR/1850 [Gene]SCO1728/1691 NC_003888 SCI11.17c, possible gntR-family transcriptional regulator, len: 134 aa; weakly similar to the N-terminal half of SW:HUTC_PSEPU (EMBL:M33922), HutC, Pseudomonas putida histidine utilization repressor (248 aa), fasta scores; opt: 164 z-score: 202.7 E(): 0.00059, 33.9% identity in 109 aa overlap. Similar to many putative transcriptional regulators e.g. SCGD3.13 (EMBL:AL096822) S.coelicolor probable gntR-family transcriptional regulator (115 aa) (32.4% identity in 111 aa overlap) and to TR:O06550 (EMBL:Z95584) Mycobacterium tuberculosis hypothetical protein (121 aa) (50.0% identity in 106 aa overlap). Contains probable helix-turn-helix motif at aa 106-127 (Score 1082, +2.87 SD). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 4 1 16 8 [Reporter]SCO0282 (15H4)_PCR/1849 [Gene]SCO0282/1690 NC_003888 SCF85.10, probable deoR family transcriptional regulator, len: 254 aa; similar to many transcriptional regulators of the deoR family e.g. Bacillus subtilis TR:P94591 (EMBL:Z83337) similar to phosphotransferase system regulator (258 aa), fasta scores opt: 433 z-score: 500.9 E(): 1.5e-20 33.5% identity in 254 aa overlap. Contains a PS00894 Bacterial regulatory proteins, deoR family signature and a Pfam match to entry PF00455 deoR, Bacterial regulatory proteins, deoR family. Has a possible helix turn helix motif between aa 23..44. 1 3 11 2 [Reporter]SCO3988 (20E14)_PCR/4253 [Gene]SCO3988/3869 NC_003888 SCBAC25E3.25, unknown, len: 115 aa: no significant database matches. 1 3 10 22 [Reporter]SCO5275 (1E10)_PCR/4251 [Gene]SCO5275/3868 NC_003888 2SC7G11.37, possible ATP/GTP binding protein, len: 1007 aa; similar to (EMBL:AL391588) Streptomyces coelicolor SCCB12.04, 1148 aa; fasta scores: opt: 4019 z-score: 3915.8 E(): 0; 62.6% identity in 994 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 10 21 [Reporter]SCO4184 (2A10)_PCR/4250 [Gene]SCO4184/3867 NC_003888 SCD66.21, amfC, aerial mycelium formation, len: 222 aa; identical to previously sequenced TR:Q54191 (EMBL:D63677) Streptomyces coelicolor aerial micelium formation AmfC, 222 aa 1 3 10 20 [Reporter]SCO4708 (3M6)_PCR/4249 [Gene]SCO4708/3866 NC_003888 SCD31.33, rpsC, 30S ribosomal protein S3, len: 277 aa; hihgly similar to SW:RS3_BACSU (EMBL:D50302) Bacillus subtilis 30S ribosomal protein S3 RpsC, 217 aa; fasta scores: opt: 801 z-score: 820.5 E(): 0; 55.1% identity in 205 aa overlap. Contains Pfam matches to entries PF00417 Ribosomal_S3_N, Ribosomal protein S3, N-terminal domain, PF00013 KH-domain, KH domain and PF00189 Ribosomal_S3_C, Ribosomal protein S3, C-terminal domain and matches to Prosite entries PS00050 Ribosomal protein L23 signature and PS00548 Ribosomal protein S3 signature 1 3 10 19 [Reporter]SCO6622 (4I6)_PCR/4248 [Gene]SCO6622/3865 NC_003888 SC1F2.19, probable ATP/GTP binding protein, len: 628 aa; very weak similarity in C-terminus to a yeast ATP /GTP binding protein TR:Q12179 (EMBL:Z73601) Saccharomyces cerevisiae chromosome XVI ORF YPL245W (454 aa), fasta scores; opt: 201 z-score: 198.6 E(): 0.00081, 25.2% identity in 469 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop) and PS00152 ATP synthase alpha and beta subunits signature. Also similar to downstream gene SC1F2.20, E(): 0, 37.1% identity in 657 aa overlap 1 3 10 18 [Reporter]SCO5724 (5E6)_PCR/4247 [Gene]SCO5724/3864 NC_003888 SC3C3.10, unknown, len: 124 aa 1 3 10 17 [Reporter]SCO2040 (6A6)_PCR/4246 [Gene]SCO2040/3863 NC_003888 SC4G6.09c, possible membrane protein, len: aa; similar to TR:O06133 (EMBL:Z95554) hypothetical protein from Mycobacterium tuberculosis (132 aa) fasta scores; opt: 245, z-score: 301.1, E(): 1.9e-09, (36.7% identity in 128 aa overlap). Contains possible membrane spanning hydrophobic regions 4 1 16 6 [Reporter]SCO0265 (16P24)_PCR/1847 [Gene]SCO0265/1689 NC_003888 SCF1.07, possible hydroxylase, len: 263 aa; similar to many from Streptomyces sp. eg. TR:Q55078 (EMBL:U50973) daunomycin C-14 hydroxylase from Streptomyces sp. strain C5 (275 aa) fasta scores; opt: 302, z-score: 356.8, E(): 1.7e-12, (39.3% identity in 270 aa overlap). Also similar to TR:CAB45588 (EMBL:AL079355) putative hydroxylase from Streptomyces coelicolor (263 aa) fasta scores; opt: 746, z-score: 870.2, E(): 0, (44.3% identity in 262 aa overlap). 1 3 10 16 [Reporter]SCO3606 (7M2)_PCR/4245 [Gene]SCO3606/3862 NC_003888 SC66T3.17, possible regulator, len: 360 aa; similar to the C-terminal region of TR:Q00509 (EMBL:X63451), SrmR, Streptomyces ambofaciens polyketide synthase regulatory gene (604 aa), fasta scores; opt: 248 z-score: 291.7 E(): 6.4e-09, 25.3% identity in 372 aa overlap. Also similar to the C-terminal region of Mycobacteria e.g. TR:O69477 (EMBL:AL023635) Mycobacterium leprae hypothetical protein (414 aa) (26.0% identity in 277 aa overlap). Weakly similar to the C-terminal region of SC8D9.30c (EMBL:AL035569) S.coelicolor possible transcriptional regulator (415 aa) (26.7% identity in 303 aa overlap). Contains probable helix-turn-helix motif at aa 237-258 (score 1012, +2.63 SD) 4 1 16 5 [Reporter]SCO3833 (17L24)_PCR/1846 [Gene]SCO3833/1688 NC_003888 SCH69.03c, possible transcriptional regulator, len: 209 aa; similar to many e.g. TR:AAD13556 (EMBL:AF080235) LanK regulator from the landomycin biosynthesis cluster of Streptomyces cyanogenus (192 aa) fasta scores; opt: 201, z-score: 245.8, E(): 2.3e-06, (33.3% identity in 159 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and probable helix-turn-helix 31-52 (+5.06 SD). 1 3 10 15 [Reporter]SCO5566 (8I2)_PCR/4244 [Gene]SCO5566/3861 NC_003888 SC7A1.10, recG, ATP-dependent DNA helicase, len: 742aa; similar to many eg. SW:RECG_ECOLI ATP-dependent DNA helicase from Escherichia coli (693 aa) fasta scores; opt: 438, z-score: 1301.5, E(): 0, (35.7% identity in 723 aa overlap). Contains PS00017 ATP /GTP-binding site motif A (P-loop) and Pfam match to entry PF00271 helicase_C, Helicases conserved C-terminal domain, score 67.50, E-value 2.8e-16. 4 1 16 3 [Reporter]SCO6305 (19D24)_PCR/1844 [Gene]SCO6305/1687 NC_003888 SCIF3.07c, conserved hypothetical protein, len: 347 aa; similar to TR:Q55861 (EMBL:D64004) Synechocystis sp. hypothetical 38.3 kDa protein SLR0619, 348 aa; fasta scores: opt: 416 z-score: 477.8 E(): 4.4e-19; 30.8% identity in 312 aa overlap 1 3 10 14 [Reporter]SCO7139 (9E2)_PCR/4243 [Gene]SCO7139/3860 NC_003888 SC8B1.03, probable aldehyde dehydrogenase (fragment), len: >469 aa; similar to SW:DHAB_RHIME (EMBL:U39940) Rhizobium meliloti betaine aldehyde dehydrogenase (EC 1.2.1.8) BetB, 487 aa; fasta scores: opt: 1021 z-score: 1101.5 E(): 0; 40.5% identity in 462 aa overlap and to TR:CAB46804 (EMBL:AL096811) Streptomyces coelicolor putative aldehyde dehydrogenase SCI30A.27c, 462 aa; fasta scores: opt: 1074 z-score: 994.9 E(): 0; 52.9% identity in 448 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family, and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00687 Aldehyde dehydrogenases glutamic acid active site,SC9A4.01c, possible dehydrogenase (fragment), len: >51 aa; highly similar to C-terminal region of SW:DHAB_BACSU (EMBL:U47861) Bacillus subtilis betaine aldehyde dehydrogenase (EC 1.2.1.8) gbsA, 490 aa; fasta scores: opt: 179 z-score: 258.5 E(): 6.3e-07; 56.0% identity in 50 aa overlap 4 1 16 2 [Reporter]SCO3056 (20L20)_PCR/1843 [Gene]SCO3056/1686 NC_003888 SCBAC19G2.11, conserved hypothetical protein, len: aa: similar to many in Streptomyces coelicolor eg. TR:Q9F3E9 (EMBL:AL450289) SC2H2.06 hypothetical protein (150 aa) fasta scores; opt: 340, Z-score: 415.4, 46.032% identity in 126 aa overlap and upstream neighbouring CDS SCBAC19G2.10 4 1 15 22 [Reporter]SCO1323 (1L16)_PCR/1841 [Gene]SCO1323/1685 NC_003888 2SCG61.05c, possible integral membrane protein, len: 258aa; similar to many eg. TR:Q9RKH4 (EMBL:AL133252) putative integral membrane protein from Streptomyces coelicolor (249 aa) fasta scores; opt: 353, z-score: 396.9, E(): 1.2e-14, 33.2% identity in 244 aa overlap. Contains possible membrane-spanning hydrophobic regions. 4 1 15 21 [Reporter]SCO2198 (2H16)_PCR/1840 [Gene]SCO2198/1684 NC_003888 SC3H12.06, glnA, glutamine synthetase I, len: 469 aa; identical to previously sequenced SW:GLNA_STRCO (EMBL:M23172) Streptomyces coelicolor glutamine synthetase (EC 6.3.1.2) (glutamate-ammonia ligase) GlnA, 469 aa and to previously sequenced TR:CAB51282 (EMBL:AL096872) Streptomyces coelicolor glutamine synthetase I SC5F7.03, 469 aa and highly similar to SW:GLN1_STRVR (EMBL:X70924) Streptomyces viridochromogenes glutamine synthetase I (EC 6.3.1.2) (glutamate-ammonia ligase I) GlnI or GlnA, 469 aa; fasta scores: opt: 2905 z-score: 3417.5 E(): 0; 90.6% identity in 469 aa overlap. Contains Pfam match to entry PF00120 gln-synt, Glutamine synthetase and three Prosite matches to entries PS00180 Glutamine synthetase signature 1, PS00181 Glutamine synthetase putative ATP-binding region signature and PS00182 Glutamine synthetase class-I adenylation site 4 1 15 20 [Reporter]SCO7630 (3D16)_PCR/1839 [Gene]SCO7630/1683 NC_003888 SC10F4.03, probable isomerase, len: 224 aa; similar to SW:PMG2_ECOLI (EMBL:M97495) Escherichia coli probable phosphoglycerate mutase 2 (EC 5.4.2.1) GpmB, 215 aa; fasta scores: opt: 190 z-score: 225.8 E(): 4.8e-05; 28.5% identity in 214 aa overlap and to TR:Q9ZAX0 (EMBL:U73808) Amycolatopsis methanolica 2,3-PDG dependent phosphoglycerate mutase PGM, 205 aa; fasta scores: opt: 518 z-score: 596.0 E(): 1.2e-25; 45.5% identity in 202 aa overlap. Contains Pfam match to entry PF00300 PGAM, Phosphoglycerate mutase family and match to Prosite entry PS00175 Phosphoglycerate mutase family phosphohistidine signature 4 1 15 19 [Reporter]SCO7034 (4P12)_PCR/1838 [Gene]SCO7034/1682 NC_003888 SC2C3.01, probable aminotransferase (fragment), len: >275 aa; similar to SW:GABT_ECOL (EMBL:M88334) Escherichia coli 4-aminobutyrate aminotransferase (EC 2.6.1.19) GabT, 426 aa; fasta scores: opt: 634 z-score: 729.2 E(): 0; 40.8% identity in 233 aa overlap. Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate and match to Prosite entry PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site,SC1H10.23, possible aminotransferase, partial CDS len: > 187 aa. Similar to Escherichia coli SW:ARGD_ECOLI (EMBL:M32796) acetylornithine aminotranferase (EC 2.6.1.11), ArgD (405 aa), fasta scores opt: 288 z-score:0.0 E(): 0.0 34.44% identity in 151 aa overlap. Also similar to many other putative aminotransferases e.g. Bacillus subtilis SW:GABT_BACSU(EMBL:D50453) probable 4-aminobutyrate aminotransferase (EC 2.6.1.19), fasta scores opt:399 z-score: 483.6 E(): 1.7e-19 36.2% identity in 177 aa overlap. Contains a Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate. 4 1 15 18 [Reporter]SCO6369 (5L12)_PCR/1837 [Gene]SCO6369/1681 NC_003888 SC4A2.05, probable two-component sensor, len: 436 aa; similar to many e.g. CUTS_STRLI CtS protein (414 aa), fasta scores; opt: 226 z-score: 309.1 E(): 5.8e-10, 32.9% identity in 316 aa overlap. Contains two probable transmembrane domains in the N-terminal half and Pfam matches to entry PF00672 DUF5, Domain found in bacterial signal proteins, score 42.70, E-value 8.2e-09 and to entry PF00512 signal, Signal carboxyl-terminal domain, score 38.00, E-value 1.7e-07 4 1 15 17 [Reporter]SCO5763 (6H12)_PCR/1836 [Gene]SCO5763/1680 NC_003888 SC4H8.02, possible membrane protein, len: 256 aa. Contains possible hydrophobic membrane spanning region 1 3 10 13 [Reporter]SCO6482 (10A2)_PCR/4242 [Gene]SCO6482/3859 NC_003888 SC9C7.18, conserved hypothetical protein, len: 348 aa; similar to two hypothetical proteins e.g. TR:O69685 (EMBL:AL022121) Mycobacterium tuberculosis RV03718c or MTV025.066c, 147 aa; fasta scores; opt: 475, z-score: 768.3, E(): 0, (52.1% identity in 146 aa overlap) 1 3 10 12 [Reporter]SCO2764 (10M22)_PCR/4241 [Gene]SCO2764/3858 NC_003888 SCC57A.35c, possible lipoprotein, len: 336 aa. Contains an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. 1 3 10 11 [Reporter]SCO4675 (11I22)_PCR/4240 [Gene]SCO4675/3857 NC_003888 SCD40A.21c, hypothetical protein, len: 302 aa; similar to TR:Q9XD94 (EMBL:AF106004) Streptomyces coelicolor hypothetical 34.1 kD protein, 304 aa; fasta scores: opt: 850 z-score: 992.1 E(): 0; 45.0% identity in 300 aa overlap. Contains match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 1 3 10 10 [Reporter]SCO3386 (12E22)_PCR/4239 [Gene]SCO3386/3856 NC_003888 SCE126.04, unknown, len: 90aa; 1 3 10 9 [Reporter]SCO0333 (13A22)_PCR/4238 [Gene]SCO0333/3855 NC_003888 SCF12.12c, possible dioxygenase (putative secreted protein), len: 503 aa; similar to many of undefined function and to TR:Q53353 (EMBL:S65040) lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (485 aa) fasta scores; opt: 529, z-score: 596.3, E(): 7.3e-26, (31.6% identity in 493 aa overlap) 1 3 10 8 [Reporter]SCO0254 (14M18)_PCR/4237 [Gene]SCO0254/3854 NC_003888 SCJ9A.33c, hypothetical protein, len: 246 aa; weakly similar to TR:Q18926 (EMBL:U23517) hypothetical protein from the eukaryote Caenorhabditis elegans (243 aa) fasta scores; opt: 354, z-score: 396.7, E(): 9.9e-15, (28.3% identity in 240 aa overlap). Also weakly similar to TR:O87611 (EMBL:AF087482) dienelactone hydrolase from Pseudomonas aeruginosa (236 aa) fasta scores; opt: 212, z-score: 241.9, E(): 4.1e-06, (27.4% identity in 230 aa overlap). Similar also to TR:Q9I4H1 (EMBL:AE004547) Pseudomonas aeruginosa hypothetical protein PA1166, 262 aa; fasta scores: opt: 474 Z-score: 515.3 E(): 4.5e-21; 35.000% identity in 240 aa overlap 1 3 10 7 [Reporter]SCO0536 (15I18)_PCR/4236 [Gene]SCO0536/3853 NC_003888 SCF11.16c, unknown, len: 73 aa 4 1 15 16 [Reporter]SCO5706 (7D12)_PCR/1835 [Gene]SCO5706/1679 NC_003888 SC9F2.10c, probable translational initiation factor, partial CDS, len: >249aa; similar to the C-terminal region of many eg. SW:IF2_BACST translational initiation factor IF2 from Bacillus stearothermophilus (741 aa) fasta scores; opt: 788, z-score: 893.1, E(): 0, (51.2% identity in 248 aa overlap).,SC5H4.30, probable translation initiation factor IF-2 (fragment), len: >835 aa; highly similar to SW:IF2_MYCLE (EMBL:AL035472) Mycobacterium leprae translation initiation factor IF-2 InfB, 924 aa; fasta scores: opt: 2559 z-score: 1220.5 E(): 0; 59.1% identity in 838 aa overlap and to SW:IF2_STIAU (EMBL:X87940) Stigmatella aurantiaca translation initiation factor IF-2 InfB, 1054 aa; fasta scores: opt: 1337 z-score: 648.2 E(): 1.2e-28; 38.8% identity in 891 aa overlap. Contains Pfam match to entry PF00009 GTP_EFTU, Elongation factor Tu family and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 10 6 [Reporter]SCO0814 (16E18)_PCR/4235 [Gene]SCO0814/3852 NC_003888 SCF43A.04, probable rhamnose kinase, len: 484 aa; similar to rhamnose kinases e.g. SW:RHAB_ECOLI (EMBL:L19201), rhaB, Escherichia coli rhamnulokinase (489 aa), fasta scores; opt: 1136 z-score: 1280.7 E(): 0, 41.5% identity in 468 aa overlap. Also similar to many other carbohydrate kinases e.g. SW:FUCK_ECOLI (EMBL:X15025), fucK, Escherichia coli L-fuculokinase (482 aa) (27.7% identity in 411 aa overlap). Contains Pfam match to entry PF00370 FGGY, FGGY family of carbohydrate kinases 4 1 15 15 [Reporter]SCO2487 (8P8)_PCR/1834 [Gene]SCO2487/1678 NC_003888 SC7A8.26, nirB, probable nitrite reductase large subunit, len: 871 aa; similar to TR:Q00943 (EMBL:Z68122) Pichia angusta nitrite reductase Yni1, 1044 aa; fasta scores: opt: 1960 z-score: 2117.6 E(): 0; 43.2% identity in 868 aa overlap and SW:NIRB_ECOLI (EMBL:X14202) Escherichia coli nitrite reductase [NAD(P)H] large subunit (EC 1.6.6.4) NirB 847 aa; fasta scores:opt: 1609 z-score: 1739.1 E(): 0; 52.1% identity in 845 aa overlap. Contains Pfam match to entry PF01077 NIR_SIR, Nitrite and sulphite reductase and two matches to Prosite entries PS00136 Serine proteases, subtilase family, aspartic acid active site and PS00365 Nitrite and sulfite reductases iron-sulfur/siroheme-binding site 1 3 10 5 [Reporter]SCO3907 (17A18)_PCR/4234 [Gene]SCO3907/3851 NC_003888 SCH24.29, ssb, probable single-strand DNA-binding protein, len: 199 aa; similar to SW:SSB_SERMA (EMBL:X65080), Ssb, Serratia marcescens single-strand binding protein (175 aa), fasta scores; opt: 278 z-score: 245.3 E(): 2.4e-06, 33.3% identity in 168 aa overlap. C-terminus contains glycine-rich repeats similar to those found in eukaryotic RNA-binding proteins e.g. SW:GRP2_SINAL (EMBL:L31377), Grp2A, Sinapis alba (white mustard) glycine-rich RNA-binding protein (169 aa). Contains Pfam match to entry PF00436 SSB, Single-strand binding protein family, score 143.30, E-value 2.5e-39. 4 1 15 14 [Reporter]SCO6984 (9L8)_PCR/1833 [Gene]SCO6984/1677 NC_003888 SC8F11.10c, possible oxidoreductase, len: 358 aa. Similar to many including: Sinorhizobium meliloti TR:O68965(EMBL:) myo-inositol dehydrogenase (EC 1.1.1.18) IdhA (330 aa), fasta scores opt: 715 z-score: 789.8 E(): 0 39.6% identity in 338 aa overlap and Streptomyces coelicolor TR:CAB60174(EMBL:AL132824) putative dehydrogenase, SCAH10.20C (337 aa), fasta scores opt: 1068 z-score: 1176.0 E(): 0 50.8% identity in 333 aa overlap. Note codon 25 offers an alternative translational start site. Contains a Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family. 1 3 10 3 [Reporter]SCO4021 (19I14)_PCR/4232 [Gene]SCO4021/3850 NC_003888 2SC10A7.25, probable two component system histidine kinase, len: 524 aa; similar to SW:AFQ2_STRCO (EMBL:D10654) Streptomyces coelicolor sensor protein AfsQ2 (EC 2.7.3.-), 535 aa; fasta scores: opt: 481 z-score: 498.8 E(): 2.8e-20; 29.0% identity in 517 aa overlap. Contains Pfam match to entry PF00672 DUF5, HAMP domain and PF00512 signal, Histidine kinase. Also contains possible N-terminal region signal peptide sequence 4 1 15 13 [Reporter]SCO7358 (10H8)_PCR/1832 [Gene]SCO7358/1676 NC_003888 SC9H11.12c, unknown, len: 500 aa. 4 1 15 12 [Reporter]SCO2546 (11D8)_PCR/1831 [Gene]SCO2546/1675 NC_003888 SCC77.13c, probable adenosine deaminase, len: 340 aa. Highly similar to many prokaryotic and Eukaryotic deaminases including: Escherichia coli SW:ADD_ECOLI (EMBL:M59033) adenosine deaminase (EC 3.5.4.4) (333 aa), fasta scores opt: 448 z-score: 533.2 E(): 2.6e-22 28.8% identity in 333 aa overlap and Saccharomyces cerevisiae (Baker's yeast) SW:ADA_YEAST (EMBL:Z46843) probable adenosine deaminase (EC 3.5.4.4) (347 aa), fasta scores opt: 860 z-score: 1017.9 E():0 44.4% identity in 322 aa overlap. Also similar to Streptomyces coelicolor TR:O86737 (EMBL:AL031035) putative adenosine deaminase SC6A9.05 (387 aa), fasta scores opt: 617 z-score: 731.1 E():0 35.9% identity in 343 aa overlap. Contains a Pfam match to entry PF00962 A_deaminase. 4 1 15 10 [Reporter]SCO3439 (13L4)_PCR/1829 [Gene]SCO3439/1674 NC_003888 SCE36.06, unknown, len: 290aa; 4 1 15 9 [Reporter]SCO3919 (14H4)_PCR/1828 [Gene]SCO3919/1673 NC_003888 SCQ11.02c, abaB, probable lysR-family transcriptional regulator, len: 301 aa; previously sequenced as TR:Q53869 (EMBL:U37580) S.coelicolor lysR-like protein (301 aa). Highly similar to SW:ARAB_STRAT (EMBL:X91393), AraB, Streptomyces antibioticus transcriptional regulatory protein (301 aa), fasta scores; opt: 1581 z-score: 1813.2 E(): 0, 80.3% identity in 300 aa overlap. Similar to many other transcriptional regulators. Contains probable helix-turn-helix motif at aa 17-38 (Score 1659, +4.84 SD). Contains Pfam match to PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and PS00044 Bacterial regulatory proteins, lysR family signature 4 1 15 8 [Reporter]SCO5216 (15D4)_PCR/1827 [Gene]SCO5216/1672 NC_003888 SC7E4.13, sigR, RNA polymerase sigma factor, len: 227 aa; identical to previously sequenced TR:O87834 (EMBL:AJ010320) Streptomyces coelicolor RNA polymerase sigma factor SigR, 227 aa. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) and match to Prosite entry PS01063 Sigma-70 factors ECF subfamily signature. Contains also possible helix-turn-helix motif at residues 171..192 (+2.53 SD) 4 1 15 7 [Reporter]SCO3742 (15P24)_PCR/1826 [Gene]SCO3742/1671 NC_003888 SCH22A.20, possible glycosyltransferase, len: 242 aa; similar to various putative glycosyltransferases, e.g. TR:CAB50363 (EMBL:AJ248287) Pyrococcus abyssi dolichol-P-glucose synthetase, 378 aa; fasta scores: opt: 330 z-score: 398.4 E(): 9e-15; 31.4% identity in 220 aa overlap 4 1 15 6 [Reporter]SCO0538 (16L24)_PCR/1825 [Gene]SCO0538/1670 NC_003888 SCF11.18, probable sugar transporter sugar binding lipoprotein, len: 449 aa; similar to TR:AAD35680 (EMBL:AE001734) Thermotoga maritima putative sugar ABC transporter periplasmic sugar-binding protein TM0595, 419 aa; fasta scores: opt: 333 z-score: 361.2 E(): 9.5e-13; 24.1% identity in 406 aa overlap. Contains a Pfam match to entry PF01547 SBP_bacterial_1, bacterial extracellular solute-binding protein and probable N-terminal signal sequence 1 3 10 2 [Reporter]SCO3051 (20A14)_PCR/4231 [Gene]SCO3051/3849 NC_003888 SCBAC19G2.06c, fadE, acyl-CoA dehydrogenase, len: 385 aa: similar to many e.g. TR:Q9RIQ5 (EMBL:AJ250495) fatty acid acyl CoA dehydrogenase FadE from Streptomyces lividans (385 aa) fasta scores; opt: 2480, Z-score: 2753.5, 99.740% identity (99.740% ungapped) in 385 aa overlap and SW:P45867 (ACDA_BACSU) acyl CoA dehydrogenase from Bacillus subtilis (379 aa) fasta scores; opt: 1214, Z-score: 1351.1, 53.600% identity (54.472% ungapped) in 375 aa overlap. Also similar to TR:Q9RKY7 (EMBL:AL133213) SC6D7.11 Acd acyl CoA dehydrogenase from Streptomyces coelicolor (391 aa) fasta scores; opt: 1079, Z-score: 1201.3, 47.244% identity (48.387% ungapped) in 381 aa overlap. Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase, C-terminal domain; Pfam match to entry PF02770 Acyl-CoA_dh_M, Acyl-CoA dehydrogenase, middle domain; Pfam match to entry PF02771 Acyl-CoA_dh_N, Acyl-CoA dehydrogenase, N-terminal domain; Prosite match to PS00072 Acyl-CoA dehydrogenases signature 1 and Prosite match to PS00073 Acyl-CoA dehydrogenases signature 2. 1 3 9 22 [Reporter]SCO5270 (1A10)_PCR/4229 [Gene]SCO5270/3848 NC_003888 2SC7G11.32, conserved hypothetical protein, len: 340 aa; similar to TR:CAB86114 (EMBL:AL163003) Streptomyces coelicolor hypothetical 37.1 kDa protein SCC24.20, 340 aa; fasta scores: opt: 1113 z-score: 1224.6 E(): 0; 54.5% identity in 343 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 9 20 [Reporter]SCO4729 (3I6)_PCR/4227 [Gene]SCO4729/3847 NC_003888 SC6G4.07, rpoA, DNA-directed RNA polymerase alpha chain, len: 340 aa; almost identical to RPOA_STRCO DNA-directed RNA polymerase alpha chain (EC 2.7.7.6) (339 aa)99.1% id entity in 340 aa overlap, and highly similar to many e.g. R POA_BACSU (314 aa), fasta scores; opt: 917 z-score: 1326.7 E(): 0, 48.9% identity in 311 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 9 19 [Reporter]SCO6043 (4E6)_PCR/4226 [Gene]SCO6043/3846 NC_003888 SC1B5.03c, probable secreted protease, len: 543 aa;similar to e.g. Streptomyces lividans TR:Q54410 (EMBL:L27466) tripeptidylaminopeptidase (537 aa), fasta scores; opt: 1839 z-score: 1633.9 E(): 0, 51.9% identity in 538 aa overlap. Contains possible N-terminal signal sequence 1 3 9 18 [Reporter]SCO5719 (5A6)_PCR/4225 [Gene]SCO5719/3845 NC_003888 SC3C3.05c, unknown, len: 266 aa 1 3 9 17 [Reporter]SCO6655 (6M2)_PCR/4224 [Gene]SCO6655/3844 NC_003888 SC5A7.05, ribA2, GTP cyclohydrolase II, len: 221 aa; highly similar to the C-terminus of many RibA genes where the GTP cyclohydrolase activity is, e.g. GCH2_BACSU GTP cyclohydrolase II (EC 3.5.4.25) (398 aa), fasta sores; opt: 656 z-score: 960.9 E(): 0, 53.1% identity in 196 aa overlap and to SW:GCH2_ARATH (EMBL:D45165) Arabidopsis thaliana GTP cyclohydrolase II (EC 3.5.4.25) 245 aa; fasta scores: opt: 656 Z-score: 760.1 E(): 1.1e-34; 51.832% identity in 191 aa overlap 1 3 9 16 [Reporter]SCO3602 (7I2)_PCR/4223 [Gene]SCO3602/3843 NC_003888 SC66T3.13c, probable transmembrane transport protein, len: 403 aa; similar to many putative ion transport proteins and to part of SW:KEFC_ECOLI (EMBL:X56742), kefC, Escherichia coli glutathione-regulated potassium-efflux system protein (620 aa), fasta scores; opt: 328 z-score: 319.2 E(): 1.9e-10, 27.1% identity in 410 aa overlap. Similar to TR:O69958 (EMBL:AL022268) S.coelicolor putative transmembrane transport protein (411 aa) (74.9% identity in 399 aa overlap). Contains hydrophobic, possible membrane-spanning regions 4 1 15 5 [Reporter]SCO1564 (17H24)_PCR/1824 [Gene]SCO1564/1669 NC_003888 SCL11.20c, possible RNA polymerase sigma factor ECF subfamily, len: 166 aa; similar to SW:RFAY_XANCP (EMBL:U19896) Xanthomonas campestris probable RNA polymerase sigma factor RfaY, 217 aa; fasta scores: opt: 135 z-score: 168.4 E(): 0.058; 30.9% identity in 136 aa overlap 1 3 9 15 [Reporter]SCO2152 (8E2)_PCR/4222 [Gene]SCO2152/3842 NC_003888 SC6G10.25, possible response regulator, len: 133aa; similar to TR:P95183 (EMBL:Z83867) hypothetical protein from Mycobacterium tuberculosis (133 aa) fasta scores; opt: 474, z-score: 602.8, E(): 2.8e-26, (54.2% identity in 120 aa overlap) and TR:E1358527 (EMBL:AJ131213) response regulator from Streptomyces coelicolor (234 aa) fasta scores; opt: 122, z-score: 162.8, E(): 0.091, (28.5% identity in 130 aa overlap) 4 1 15 4 [Reporter]SCO3789 (18D24)_PCR/1823 [Gene]SCO3789/1668 NC_003888 SCH63.36, possible integral membrane protein, len: 338 aa; similar to TR:Q9RS34 (EMBL:AE002061) Deinococcus radiodurans conserved hypothetical protein DR2293, 322 aa; fasta scores: opt: 233 z-score: 262.7 E(): 4.1e-07; 34.9% identity in 304 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 9 14 [Reporter]SCO6603 (9A2)_PCR/4221 [Gene]SCO6603/3841 NC_003888 SC8A6.24, probable transmembrane sugar transport pro tein, len: 279 aa; similar to e.g. S. coelicolor TR:P72399 (EMBL:Y07706) putative maltose permease malG (302 aa), fast a scores; opt: 542 z-score: 755.1 E(): 0, 36.7% identity in 270 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature and Pfam m atch to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 75.20, E -value 1.3e-18 4 1 15 3 [Reporter]SCP1.100 (19P20)_PCR/1822 [Gene]SCP1.100/1667 NC_003888 SCP1.100, possible ATP/GTP-binding Gly/Ala-rich protein, len: 811aa; contains possible coiled-coil region at 428-444 residues. Also contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 9 12 [Reporter]SCO2760 (10I22)_PCR/4219 [Gene]SCO2760/3840 NC_003888 SCC57A.31, possible membrane protein, len: 216 aa. Contains a possible membrane spanning hydrophobic domain. 4 1 15 2 [Reporter]SCO7507 (20H20)_PCR/1821 [Gene]SCO7507/1666 NC_003888 SCBAC17A6.40c, possible dioxygenase, len: 315 aa; similar to many eg. SW:P37610 (TAUD_ECOLI) alpha-ketoglutarate-dependent taurine dioxygenase from Escherichia coli (282 aa) fasta scores: opt: 786, Z-score: 906.3, 44.245% identity (45.556% ungapped) in 278 aa overlap. Contains Pfam match to entry PF02668 TauD, Taurine catabolism dioxygenase TauD, TfdA family. 4 1 14 21 [Reporter]SCO7338 (2D16)_PCR/1818 [Gene]SCO7338/1665 NC_003888 SC4G10.17, glgX, possible glycogen debranching enzyme, len: 715 aa. Previously sequenced region: Streptomyces coelicolor TR:Q9X947(EMBL:AJ001206) (715 aa). Highly similar to Escherichia coli SW:GLGX_ECOLI(EMBL:J01616) glycogen operon protein, GlgX (EC 3.2.1.-) (657 aa), fasta scores opt: 1948 z-score: 2333.6 E():0 47.5% identity in 673 aa overlap and Streptomyces coelicolor TR:CAB76010(EMBL:AL157916) putative glycosyl hydrolase, GlgX2 or SC3D11.13c (782 aa), fasta scores opt: 2622 z-score: 3141.4 E(): 0 58.5% identity in 725 aa overlap. Contains a Pfam match to entry PF00128 alpha-amylase, Alpha amylase. 4 1 14 20 [Reporter]SCO5930 (3P12)_PCR/1817 [Gene]SCO5930/1664 NC_003888 SC10A5.35, possible NTP pyrophosphohydrolase, len: 3 59 aa; similar to TR:Q53738 (EMBL:X92429) NTP pyrophosphohydrolase PUR7 from S. alboniger (152 aa), fasta scores; opt: 467 z-score: 614.5 E(): 5e-27, 54.8% identity in 135 aa overlap. Contains PS00893 mutT domain signature 4 1 14 19 [Reporter]SCO7030 (4L12)_PCR/1816 [Gene]SCO7030/1663 NC_003888 SC1H10.19, possible binding-protein-dependent transport protein, len: 310 aa. Almost identical to Streptomyces lividans TR:Q9ZI16 (EMBL:AF043654) putative permease, BxlG (272 aa), fasta scores opt: 1714 z-score: 2067.9 E():0 98.5% identity in 272 aa overlap. Also similar to Streptococcus mutans SW:MSMG_STRMU(EMBL:M77351) multiple sugar-binding transport system permease protein, MsmG (277 aa), fasta scores opt: 441 z-score: 537.9 E(): 1.5e-22 28.0% identity in 254 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and multiple possible membrane spanning hydrophobic domains. Also contains a possible N-terminal signal sequence. 4 1 14 18 [Reporter]SCO5465 (5H12)_PCR/1815 [Gene]SCO5465/1662 NC_003888 SC3D11.22, unknown, len: 220aa; similar to TRNEW:CAB61931 (EMBL:AL133278) hypothetical protein from Streptomyces coelicolor (219 aa) fasta scores; opt: 519, z-score: 572.9, E(): 1.7e-24, 45.5% identity in 200 aa overlap and TR:O29370 (EMBL:AE001042) hypothetical protein from Archaeoglobus fulgidus fasta scores; opt: 181, z-score: 208.1, E(): 0.00035, 26.2% identity in 206 aa overlap. also to TR:Q98N90 (EMBL:AP002994) Rhizobium loti (Mesorhizobium loti) MLL0243 protein, 231 aa; fasta scores: opt: 789 Z-score: 849.5 E(): 1.1e-39; 56.164% identity in 219 aa overlap 4 1 14 17 [Reporter]SCO5808 (6D12)_PCR/1814 [Gene]SCO5808/1661 NC_003888 SC4H2.29, unknown, len: 219 aa; highly similar to several eukaryotic hypothetical proteins e.g. Arabidopsis thaliana TR:O04035 (EMBL:AC000106) F7G19.18 (281 aa), fasta scores; opt: 482 z-score: 1008.5 E(): 0, 47.0% identity in 217 aa overlap. Contains PS00175 Phosphoglycerate mutase family phosphohistidine signature and Pfam match to entry PGAM PF00300, Phosphoglycerate mutase family, score 20.30 4 1 14 16 [Reporter]SCO6773 (7P8)_PCR/1813 [Gene]SCO6773/1660 NC_003888 SC6A5.22, possible secreted peptidase, len: 312aa; TR:E1355668 (EMBL:AL034355) putative secreted peptidase from Streptomyces coelicolor (349 aa) fasta scores; opt: 452, z-score: 416.6, E(): 6.6e-16, (34.5% identity in 319 aa overlap) and SW:ALE1_STACP glycylglycine endopeptidase from Staphylococcus capitis (362 aa) fasta scores; opt: 332, z-score: 309.4, E(): 6.2e-10, (35.2% identity in 196 aa overlap). Contains probable N-terminal signal sequence. 1 3 9 11 [Reporter]SCO4088 (11E22)_PCR/4218 [Gene]SCO4088/3839 NC_003888 SCD25.24c, unknown, len: 84. Highly similar in N-terminus to Mycobacterium tuberculosis TR: O53824 (EMBL; AL022004) hypothetical 6.9 KD protein (60 aa), fasta scores opt: 180 z-score: 231.6 E(): 1.5e-05 51.8% identity in 56 aa overlap. 1 3 9 10 [Reporter]SCO2118 (12A22)_PCR/4217 [Gene]SCO2118/3838 NC_003888 SCE10.12c, dldh2, probable D-lactate dehydrogenase, len: 331 aa; highly similar to many members of the D-isomer specific 2-hydroxyacid dehydrogenases family, especially lactate dehydrogenases e.g. SW:LDHD_ECOLI (EMBL:U36928), LdhA, Escherichia coli D-lactate dehydrogenase (329 aa), fasta scores; opt: 843 z-score: 1010.7 E(): 0, 44.4% identity in 320 aa overlap. Similar to other dehydrogenases from S.coelicolor e.g. SC66T3.05 (EMBL:AL079348), dldh, S.coelicolor probable D-lactate dehydrogenase 337 aa) (36.3% identity in 325 aa overlap). Contains Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases 1 3 9 9 [Reporter]SCO0134 (13M18)_PCR/4216 [Gene]SCO0134/3837 NC_003888 SCJ21.15c, unknown, len: 101 aa 1 3 9 8 [Reporter]SCO1522 (14I18)_PCR/4215 [Gene]SCO1522/3836 NC_003888 SCL2.12c, hypothetical protein, len: 202 aa; similar to TR:Q49637 (EMBL:U00011) Mycobacterium leprae HisH protein, 219 aa; fasta scores: opt: 715 z-score: 849.9 E(): 0; 58.9% identity in 192 aa overlap and to TR:AAF10937 (EMBL:AE001982) Deinococcus radiodurans amidotransferase HisH, putative, 196 aa; fasta scores: opt: 508 z-score: 607.8 E(): 1.8e-26; 47.3% identity in 186 aa overlap. Contains Pfam match to entry PF01174 UPF0030, Uncharacterized protein family UPF0030 1 3 9 7 [Reporter]SCO5488 (15E18)_PCR/4214 [Gene]SCO5488/3835 NC_003888 SC2A11.22, trmU, probable tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase, len: 376 aa; similar to many e.g. TRMU_BACSU probable tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase (370 aa), fasta scores; opt: 708 z-score: 748.3 E(): 0, 38.1% identity in 365 aa overlap 1 3 9 6 [Reporter]SCO3885 (16A18)_PCR/4213 [Gene]SCO3885/3834 NC_003888 StH24.07, conserved hypothetical GidB-family protein, len: 239 aa; previously sequenced as TR:O54571 (EMBL:Y16311) Streptomyces coelicolor gidB-like protein (239 aa). Similar to many members of the gidB family of hypothetical proteins e.g. TR:O53597 (EMBL:AL021426) Mycobacterium tuberculosis hypothetical protein (224 aa), fasta scores; opt: 654 z-score: 756.9 E(): 0, 48.0% identity in 221 aa overlap 1 3 9 5 [Reporter]SCO3231 (17M14)_PCR/4212 [Gene]SCO3231/3833 NC_003888 SCE63.02c, cdaPS2, CDA peptide synthetase II, len: 3670 aa; part of the calcium-dependent antibiotic (CDA) biosynthetic cluster from Streptomyces coelicolor. CDA is a peptide antibiotic which is synthesised non-ribosomally by a putative multifunctional peptide synthetase enzyme. This CDS encodes a subunit of this enzyme and is suspected to be responsible for the addition of 3 amino acids to the peptide antibiotic. This ORF overlaps the upstream (cdaPSI) and downstream (cdaPSIII) ORFs by one base indicating possible translational coupling of all three. Contains four separate Pfam matches to entry PF00668 DUF4, Domain of unknown function (U), three separate Pfam matches to entry PF00501 AMP-binding, AMP-binding enzyme (A) and three separate Pfam matches to entry PF00550 pp-binding, Phosphopantetheine attachment site (P). These Pfam matches cover the full length of the protein in the following order from N to C-terminal U-A-P-U-A-P-U-A-P-U. The first and third Pfam matches to entry PF00501 also contain Prosite matches to PS00455 Putative AMP-binding domain signature. All three Pfam matches to entry PF00550 also contain Prosite matches to PS00012 Phosphopantetheine attachment site. 4 1 14 15 [Reporter]SCO2483 (8L8)_PCR/1812 [Gene]SCO2483/1659 NC_003888 SC7A8.22, possible secreted protein, len: 236 aa. Contains possible N-terminal region signal peptide sequence 1 3 9 4 [Reporter]SCP1.300 (18I14)_PCR/4211 [Gene]SCP1.300/3832 NC_003888 SCP1.300, possible acetaldehyde dehydrogenase (acylating), len: 293aa; similar to many eg. TR:Q51962 (EMBL:U13232) acetaldehyde dehydrogenase (acylating) from Pseudomonas putida (307 aa) fasta scores; opt: 550, z-score: 658.2, E(): 3.4e-29, 35.0% identity in 294 aa overlap. 4 1 14 14 [Reporter]SCO5492 (9H8)_PCR/1811 [Gene]SCO5492/1658 NC_003888 SC8D9.04, probable oxidoreductase, len: 232aa; similar to many eg. TR:Q53882 (EMBL:U43704) aklaviketone reductase from Streptomyces sp. strain C5 (251 aa) fasta scores; opt: 319, z-score: 361.3, E(): 8.1e-13, (33.3% identity in 237 aa overlap). Contains PS00061 Short-chain dehydrogenases/reductases family signature. Also contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase, score 129.20, E-value 7.5e-35. 1 3 9 3 [Reporter]SCO4036 (19E14)_PCR/4210 [Gene]SCO4036/3831 NC_003888 2SCD60.02c, hypothetical protein, len: 97 aa; identical to previously sequenced SW:YRPX_STRCO (EMBL:L11648) Streptomyces coelicolor hypothetical 11.1 kDa protein in RpoX 5' region, 97 aa 4 1 14 13 [Reporter]SCO7354 (10D8)_PCR/1810 [Gene]SCO7354/1657 NC_003888 SC9H11.08, unknown, len: 802 aa. Similar to many proteins of undefined function e.g. Streptomyces coelicolor TR:CAB61537(EMBL:AL133171) hypothetical 89.7 kd protein, SCF81.26 (835 aa), fasta scores opt: 2446 z-score: 2641.1 E(): 0 53.0% identity in 800 aa overlap. Contains Pfam matches to 2xentry PF00989 PAS, PAS domain. 1 3 9 2 [Reporter]SCO3362 (20M10)_PCR/4209 [Gene]SCO3362/3830 NC_003888 SCE94.13, putative membrane protein, len: 211 aa; weakly similar to many eg. SW:YRHP_BACSU hypothetical protein located close to sigma factor genes in Bacillus subtilis (210 aa) fasta scores; opt: 192, z-score: 222.0, E(): 4.7e-05, (25.0% identity in 164 aa overlap). Contains a possible membrane spanning hydrophobic regions 4 1 14 12 [Reporter]SCO5283 (11P4)_PCR/1809 [Gene]SCO5283/1656 NC_003888 SCCB12.07c, possible two-component system response regulator, len: 244 aa; similar to TR:Q9ZEP4 (EMBL:AJ131213) Streptomyces coelicolor response regulator CseB or SCE94.09, 234 aa; fasta scores: opt: 632 z-score: 771.1 E(): 0; 44.3% identity in 230 aa overlap and to SW:RESD_BACSU (EMBL:L09228), 240 aa; fasta scores: opt: 573 z-score: 699.9 E(): 1.6e-31; 39.7% identity in 234 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 4 1 14 11 [Reporter]SCO3937 (12L4)_PCR/1808 [Gene]SCO3937/1655 NC_003888 SCD78.04c, possible integrase/ recombinase within putative integrated plasmid, len: 495 aa; similar to TR:Q37839 (EMBL:D38173) ORF469 protein (possible site-specific recombinase) from Actinophage R4 (469 aa), fasta scores; opt: 261 z-score: 320.1 E(): 1.4e-10, 29.1% identity in 306 aa overlap and TR:O06604 (EMBL:Z95586) MTCY336.18 (Rv1586c) possible integrase from M. tuberculosis integrated phage phiRv1 (469 aa), fasta scores; opt: 371 z-score: 270.8 E(): 7.8e-08, (26.6% identity in 489 aa overlap). Probable coiled-coil from 414 to 451 (38 residues) Max score: 1.608 (probability 0.99) 4 1 14 10 [Reporter]SCO3205 (13H4)_PCR/1807 [Gene]SCO3205/1654 NC_003888 SCE22.22, probable MarR-family transcriptional repressor, len: 163 aa; similar to TR:Q9X5U1 (EMBL:AF127374) Streptomyces lavendula repressor MmcW, 163 aa; fasta scores: opt: 724 z-score: 892.8 E(): 0; 65.4% identity in 162 aa overlap and to TR:CAB70635 (EMBL:AL137242) Streptomyces coelicolor putative transcriptional regulator SC8F4.09c, 158 aa; fasta scores: opt: 409 z-score: 459.2 E(): 4.8e-20; 43.2% identity in 146 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 4 1 14 9 [Reporter]SCO1076 (14D4)_PCR/1806 [Gene]SCO1076/1653 NC_003888 SCG22.22c, possible secreted protein, len: 64 aa. Contains possible N-terminal region signal peptide sequence 4 1 14 8 [Reporter]SCO3419 (14P24)_PCR/1805 [Gene]SCO3419/1652 NC_003888 SCE9.26c, hypothetical protein, len: 213 aa; unknown function, weakly similar to TR:P7170 (EMBL:Z80775) Mycobacterium tuberculosis hypothetical protein (180 aa), fasta scores; opt: 154 z-score: 195.5 E(): 0.0014, 27.0% identity in 178 aa overlap. The start codon is uncertain as there are 2 potential 4 1 14 7 [Reporter]SCO1023 (15L24)_PCR/1804 [Gene]SCO1023/1651 NC_003888 SCG20A.03, possible membrane protein, len: 202 aa; similar to TR:Q9RIY8 (EMBL:AL109962) Streptomyces coelicolor hypothetical 20.8 kD protein, SCJ1.26, 193 aa; fasta scores: opt: 201 z-score: 245.7 E(): 3.3e-06; 35.4% identity in 195 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 14 6 [Reporter]SCO0754 (16H24)_PCR/1803 [Gene]SCO0754/1650 NC_003888 SCF81.13, putative secreted protein, len: 268 aa. Contains possible N-teminal signal sequence peptide 1 3 8 22 [Reporter]SCO4217 (1M6)_PCR/4207 [Gene]SCO4217/3829 NC_003888 2SCD46.31c, conserved hypothetical protein, len: 294 aa; similar to TR:P75863 (EMBL:AE000196) hypothetical protein from Escherichia coli (369 aa) fasta scores; opt: 244, z-score: 297.9, E(): 4e-09, 33.3% identity in 288 aa overlap. 1 3 8 21 [Reporter]SCO2618 (2I6)_PCR/4206 [Gene]SCO2618/3828 NC_003888 SCC80.03c, clpP2, ATP dependent Clp protease proteolytic subunit 2, len: 236 aa; identical to previously sequenced TR:Q9ZH58 (EMBL:AF071885) Streptomyces coelicolor ATP dependent Clp protease proteolytic subunit 2 (EC 3.4.21.92) ClpP2, 236 aa. Contains Pfam match to entry PF00574 CLP_protease, Clp protease and match to Prosite entry PS00381 Endopeptidase Clp serine active site 1 3 8 20 [Reporter]SCO4777 (3E6)_PCR/4205 [Gene]SCO4777/3827 NC_003888 SCD63.09, pkaD, protein serine/threonine kinase, len: 599 aa; identical to previously sequenced TR:O83032 (EMBL:AB016932) Streptomyces coelicolor protein serine/threonine kinase PkaD. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and matches to Prosite entries PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature and possible hydrophobic membrane spanning region 1 3 8 19 [Reporter]SCO6927 (4A6)_PCR/4204 [Gene]SCO6927/3826 NC_003888 SC1B2.33c, unknown, len: 374 aa. 1 3 8 18 [Reporter]SCO6847 (5M2)_PCR/4203 [Gene]SCO6847/3825 NC_003888 SC3D9.15c, unknown, len: 206 aa. Contains a 24 amino acid direct repeat (ATYRTVSRRLSRRLASFSARAEDR) between residues 129..152 and 154..177. 1 3 8 17 [Reporter]SCO5778 (6I2)_PCR/4202 [Gene]SCO5778/3824 NC_003888 SC4H8.17, two-component system transcriptional regulator, len: 228 aa; similar to many eg. VANR_ENTFA Q47744 regulatory protein VanR from enterococcuus faecalis (220 aa), fasta scores; opt: 469 z-score: 742.2 E(): 0, 37.1% identity in 224 aa overlap 1 3 8 16 [Reporter]SCO0305 (7E2)_PCR/4201 [Gene]SCO0305/3823 NC_003888 SC5G9.14, hypothetical protein, len: 959 aa; unknown function, similar to TR:O54182 (EMBL:AL021411) Streptomyces coelicolor hypothetical protein (913 aa), fasta scores; opt: 698 z-score: 776.6 E(): 0, 27.0% identity in 972 aa overlap. The C-terminus shows very weak similarity to eukaryotic beta-transducins e.g. SW:GBB4_MOUSE (EMBL:S86124), gnb4, Mus musculus guanine nucleotide-binding protein beta subunit 4 (340 aa) (23.0% identity in 291 aa overlap). Contains possible hydrophobic membrane spanning region 4 1 14 5 [Reporter]SCO3338 (17D24)_PCR/1802 [Gene]SCO3338/1649 NC_003888 SCE7.05c, probable integral membrane protein, len: 266aa; similar to many hypothetical proteins eg. TR:O33188 (EMBL:Z98268) from Mycobacterium tuberculosis (226 aa) fasta scores; opt: 661, z-score: 725.6, E(): 4.3e-33, (45.1% identity in 226 aa overlap). Contains possible membrane spanning hydrophobic regions. 1 3 8 15 [Reporter]SCO2148 (8A2)_PCR/4200 [Gene]SCO2148/3822 NC_003888 SC6G10.21c, qcrB, cytochrome B subunit, len: 545 aa; almost identical to one previously sequenced in Streptomyces lividans TR:G4154324 (EMBL:AF107888) (549 aa) fasta scores: opt: 3501, z-score: 3791.6, E(): 0, (95.5% identity in 550 aa overlap). Also similar to SW:QCRB_BACST cytochrome B subunit from Bacillus stearothermophilus (224 aa) fasta scores; opt: 409, z-score: 451.8, E(): 7.2e-18, (30.0% identity in 240 aa overlap). Contains Pfam match to entry PF00033 cytochrome_b_N, Cytochrome b(N-terminal)/b6/petB. Also contains several probable membrane spanning hydrophobic regions. 4 1 14 4 [Reporter]SCP1.266c (18P20)_PCR/1801 [Gene]SCP1.266c/1648 NC_003888 SCP1.266c, unknown, len: 150aa; 1 3 8 14 [Reporter]SCO7196 (8M22)_PCR/4199 [Gene]SCO7196/3821 NC_003888 SC8A11.24c, possible ion transport integral membrane protein, len: 288 aa; similar to SW:KCSA_STRLI (EMBL:Z37969) Streptomyces lividans voltage-gated potassium channel KcsA, 160 aa; fasta scores: opt: 302 z-score: 373.1 E(): 2.6e-13; 41.0% identity in 156 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 14 3 [Reporter]SCP1.142 (19L20)_PCR/1800 [Gene]SCP1.142/1647 NC_003888 SCP1.142, unknown, len: 87aa; 1 3 8 13 [Reporter]SCO1485 (9I22)_PCR/4198 [Gene]SCO1485/3820 NC_003888 SC9C5.09c, possible integral membrane protein, len: 191 aa; similar to TR:P71810 (EMBL:Z81011) Mycobacterium tuberculosis hypothetical 18.2 kD protein, 165 aa; fasta scores: opt: 301 z-score: 362.9 E(): 9.7e-13; 33.1% identity in 148 aa overlap. Contains possible hydrophobic membrane spanning region 4 1 14 2 [Reporter]SCO7705 (20D20)_PCR/1799 [Gene]SCO7705/1646 NC_003888 SCBAC12C8.06, possible oxidoreductase, len: 396aa: similar to many eg. SW:Q01911 (TETX_BACFR) tetracycline resistance oxidoreductase from Bacteroides fragilis (388 aa) fasta scores; opt: 612, Z-score: 673.8, 31.818% identity (33.427% ungapped) in 374 aa overlap and TR:Q9F3M8 (EMBL:AL450350) putative oxidoreductase SC10F4.32c from Streptomyces coelicolor (373 aa) fasta scores; opt: 1391, Z-score: 1524.2, 59.116% identity (60.795% ungapped) in 362 aa overlap. Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase. 4 1 13 22 [Reporter]SCO1011 (1D16)_PCR/1797 [Gene]SCO1011/1645 NC_003888 2SCG2.24c, hypothetical protein, len: 634 aa; similar to TR:Q00509 (EMBL:X6451) Streptomyces ambofaciens hypothetical SrmR protein, 604 aa; fasta scores: opt: 952 z-score: 1028.3 E(): 0; 36.5% identity in 600 aa overlap. Contains Pfam match to entry PF01590 GAF, GAF domain 4 1 13 21 [Reporter]SCO5141 (2P12)_PCR/1796 [Gene]SCO5141/1644 NC_003888 SCP8.04c, folP, dihydropteroate synthase, len: 294 aa; similar to SW:DHPS_MYCLE (EMBL:U15180) Mycobacterium leprae dihydropteroate synthase (EC 2.5.1.15) (DHPS) FolP, 291 aa; fasta scores: opt: 1095 z-score: 1239.8 E(): 0; 60.6% identity in 292 aa overlap. Contains Pfam match to entry PF00809 DHPS, Dihydropteroate synthase and matches to Prosite entries PS00792 Dihydropteroate synthase signature 1 and PS00793 Dihydropteroate synthase signature 2 4 1 13 20 [Reporter]SCO5926 (3L12)_PCR/1795 [Gene]SCO5926/1643 NC_003888 SC10A5.31c, unknown, len: 173 aa; highly similar to the C-terminus of many prokaryotic and eukaryotic aconitase s eg. ACO1_ECOLI P25516 aconitate hydratase 1 (ec 4.2.1.3) (890 aa), fasta scores; opt: 422 z-score: 567.1 E(): 2.2e-2 4, 49.3% identity in 138 aa overlap 4 1 13 19 [Reporter]SCO6030 (4H12)_PCR/1794 [Gene]SCO6030/1642 NC_003888 SC1C3.18c, unknown, len: 238 aa; similar to M. tuberculosis hypothetical protein TR:E1237486 (EMBL:AL021186) MTV010.04 (244 aa), fasta scores; opt: 516 z-score: 539.2 E(): 8.4e-23, 41.7% identity in 223 aa overlap 4 1 13 18 [Reporter]SCO5461 (5D12)_PCR/1793 [Gene]SCO5461/1641 NC_003888 SC3D11.18, possible secreted protein, len: 204aa; contains a possible non-cleavable N-terminal signal sequence. 4 1 13 17 [Reporter]SCO7453 (6P8)_PCR/1792 [Gene]SCO7453/1640 NC_003888 SC5C11.10c, putative secreted protein, len: 356 aa. Contains possible N-terminal region signal peptide sequence 1 3 8 12 [Reporter]SCO2430 (10E22)_PCR/4197 [Gene]SCO2430/3819 NC_003888 SCC24.01, possible sugar hydrolase (fragment) (putative secreted protein), len: >407 aa; similar to C-terminal region of SW:BGAL_ASPNG (EMBL:L06037) Aspergillus niger beta galactosidase precursor (EC 3.2.1.23) LacA, 1006 aa; fasta scores: opt: 214 z-score: 223.7 E(): 5e-05; 27.1% identity in 321 aa overlap,SCC42.11, probable secreted beta-galactosidase (fragment), len: >555 aa; similar to SW:BGAL_ASPNG (EMBL:L06037) Aspergillus niger beta-galactosidase precursor (EC 3.2.1.23) LacA, 1006 aa; fasta scores: opt: 413 z-score: 403.4 E(): 5.4e-15; 24.3% identity in 608 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 8 11 [Reporter]SCO4082 (11A22)_PCR/4196 [Gene]SCO4082/3818 NC_003888 SCD25.18, possible secreted peptidase, len: 279 aa. Shares a very low level of similarity, in parts, with two other putative Streptomyces coelicolor peptidases: TR:Q9ZBY3 (EMBL; AL034355) SCD78.16 (349 aa), fasta scores opt: 199 z-score: 225.8 E(): 3.2e-05 44.3% identity in 79 aa overlap and TR:CAB40868 (EMBL; AL049628) SCE94.19C (228 aa), fasta scores opt: 191 z-score: 219.8 E(): 6.8e-05 41.7% identity in 72 aa overlap. Contains a Pfam match to entry PF01551 Peptidase_M37 and a possible N-terminal signal sequence. 1 3 8 10 [Reporter]SCO2920 (12M18)_PCR/4195 [Gene]SCO2920/3817 NC_003888 SCE19A.20c, probable secreted protease, len: 781 aa; similar to SW:INA_BACTL (EMBL:X55436), Ina, Bacillus thuringiensis inhibitor A (a protease) (687 aa), fasta scores; opt: 924 z-score: 991.3 E(): 0, 32.9% identity in 747 aa overlap and TR:O07351 (EMBL:Y00557), PrtV, Vibrio cholerae protease precursor (919 aa) (33.2% identity in 794 aa overlap). Contains probable N-terminal signal sequence and PS00142 Neutral zinc metallopeptidases, zinc-binding region signature 1 3 8 9 [Reporter]SCO0620 (13I18)_PCR/4194 [Gene]SCO0620/3816 NC_003888 SCF56.04c, hypothetical protein, len: 92 aa; similar to various hypothetical proteins, e.g. TR:BAA86632 (EMBL:AB033763) Staphylococcus aureus hypothetical 9.9 kD protein, 88 aa; fasta scores: opt: 203 z-score: 280.2 E(): 3.2e-08; 43.7% identity in 71 aa overlap 1 3 8 8 [Reporter]SCO3826 (14E18)_PCR/4193 [Gene]SCO3826/3815 NC_003888 SCGD3.27c, possible membrane protein, len: 365 aa; unknown function, weakly similar to C-terminal half of SW:KEFC_ECOLI (EMBL:X56742), KefC, Escherichia coli glutathione-regulated potassium-efflux system protein (620 aa), fasta scores; opt: 164 z-score: 190.7 E(): 0.0027, 21.4% identity in 308 aa overlap. Highest similarity to TR:O53346 (EMBL:AL021646) Mycobacterium tuberculosis hypothetical protein (355 aa) (49.8% identity in 325 aa overlap). Also weakly similar to C-terminal half of SC66T3.20, S.coelicolor possible membrane protein (623 aa) (24.6% identity in 329 aa overlap) and shares regions of local similarity with other putative S.coelicolor membrane proteins. Contains hydrophobic, possible membrane-spanning regions 1 3 8 7 [Reporter]SCO1078 (15A18)_PCR/4192 [Gene]SCO1078/3814 NC_003888 SCG22.24, probable lacI-family transcriptional regulator, len: 349 aa; similar to TR:Q9S1T0 (EMBL:AL109950) Streptomyces coelicolor putative lacI-family transcriptional regulator SCJ4.43c, 367 aa; fasta scores: opt: 786 z-score: 894.0 E(): 0; 38.5% identity in 353 aa overlap and to SW:GALR_ECOLI (EMBL:V00280) Escherichia coli galactose operon repressor GalR, 343 aa; fasta scores: opt: 504 z-score: 575.9 E(): 1.3e-24; 31.3% identity in 336 aa overlap. Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and match to Prosite entry PS00356 Bacterial regulatory proteins, lacI family signature. Contains also a possible helix-turn-helix motif at residues 29..50 (+4.16 SD) 1 3 8 6 [Reporter]SCO3152 (16M14)_PCR/4191 [Gene]SCO3152/3813 NC_003888 SCE87.03c, unknown, len: 145 aa. 4 1 13 16 [Reporter]SCO6769 (7L8)_PCR/1791 [Gene]SCO6769/1639 NC_003888 SC6A5.18, probable aminotransferase, len: 461 aa; similar to many e.g. SW:ARGD_BACSU acetylornithine aminotransferase from Bacillus subtilis (385 aa) fasta scores; opt: 663, z-score: 757.6, E(): 0, (37.8% identity in 341 aa overlap). Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate and Prosite match to PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. 1 3 8 5 [Reporter]SCO3541 (17I14)_PCR/4190 [Gene]SCO3541/3812 NC_003888 SCH5.03c, possible DNA polymerase, len: 401 aa; limited region of similarity to many DNA polymerase III subunits e.g. SW:DP3X_SALTY DNA polymerase III subunits gamma and tau from Salmonella typhimurium (642 aa) fasta scores; opt: 327, z-score: 338.5, E(): 1.5e-11, (32.1% identity in 246 aa overlap). Contains probable coiled-coil region from 245 to 285 (41 residues), max score: 1.601, (probability 0.99) 4 1 13 15 [Reporter]SCO5323 (8H8)_PCR/1790 [Gene]SCO5323/1638 NC_003888 SC6G9.10, probable Asn-C family transcriptional regulator, len: 164 aa; similar to many e.g. TR:P94329 (EMBL:U85623), Lrp, Bradyrhizobium japonicum leucine-responsive regulatory protein (153 aa), fasta scores; opt: 211 z-score: 261.3 E(): 3.2e-07, 30.8% identity in 143 aa overlap. Similar to others from S.coelicolor e.g. (EMBL:AL049628), SCE94.12c, LrpA, probable AsnC-family transcriptional regulator (150 aa) (28.0% identity in 150 aa overlap). Contains probable helix-turn-helix motif at aa 24-45 (Score 1012, +2.63 SD). Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family 1 3 8 4 [Reporter]SCO1589 (18E14)_PCR/4189 [Gene]SCO1589/3811 NC_003888 SCI35.11, hypothetical protein, len: 64 aa; similar to two small hypothetical proteins from M. tuberculosis e.g. YU07_MYCTU MTCY339.07C (80 aa), fasta scores; opt: 169 z-score: 320.8 E(): 1.3e-10, 46.6% identity in 58 aa overlap 4 1 13 14 [Reporter]SCO7751 (9D8)_PCR/1789 [Gene]SCO7751/1637 NC_003888 SC8D11.42, possible regulatory protein, len: 168 aa; similar to TR:P71672 (EMBL:Z80108) Mycobacterium tuberculosis hypothetical 17.5 kDa protein CY21B4.21 precursor, 160 aa; fasta scores: opt: 206 z-score: 269.5 E(): 1.8e-07; 27.8% identity in 144 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 1 3 8 3 [Reporter]SCP1.223c (19A14)_PCR/4188 [Gene]SCP1.223c/3810 NC_003888 SCP1.223c, unknown, len: 117aa; 4 1 13 13 [Reporter]SCO2784 (10P4)_PCR/1788 [Gene]SCO2784/1636 NC_003888 SCC105.15, probable aceytltranferase, len: 184 aa; similar to SW:RHSD_RHIME (EMBL:AF110737) Rhizobium meliloti rhizobactin siderophore biosynthesis protein RhsD, 196 aa; fasta scores: opt: 257 z-score: 326.7 E(): 9.2e-11; 33.7% identity in 172 aa overlap and to TR:AAD46626 (EMBL:AF162771) Pseudomonas aeruginosa aminoglycoside 6'-N-acetyltransferase Aac, 193 aa; fasta scores: opt: 175 z-score: 226.3 E(): 3.6e-05; 28.2% identity in 174 aa overlap 4 1 13 12 [Reporter]SCO5279 (11L4)_PCR/1787 [Gene]SCO5279/1635 NC_003888 SCCB12.03, unknown, len: 412 aa 4 1 13 11 [Reporter]SCO4791 (12H4)_PCR/1786 [Gene]SCO4791/1634 NC_003888 SCD63A.02, possible two-component system sensor kinase, len: 430 aa; C-terminal domain similar to TR:CAB51969 (EMBL:AL109661) Streptomyces coelicolor putative two-component system sensor kinase SC6E10.15c, 413 aa; fasta scores: opt: 168 z-score: 198.3 E(): 0.0014; 32.7% identity in 248 aa overlap. Contains possible hydrophobic membrane regions in N-terminal domain 4 1 13 10 [Reporter]SCO3199 (13D4)_PCR/1785 [Gene]SCO3199/1633 NC_003888 SCE22.16c, probable transmembrane efflux protein, len: 505 aa; similar to SW:AC22_STRCO (EMBL:M64683) Streptomyces coelicolor probable actinorhodin transporter ActII-2, 578 aa; fasta scores: opt: 1347 z-score: 1470.8 E(): 0; 45.9% identity in 442 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and also possible hydrophobic membrane spanning regions 4 1 13 8 [Reporter]SCO0264 (14L24)_PCR/1783 [Gene]SCO0264/1632 NC_003888 SCF1.06, possible transcriptional regulator, len: 150 aa; weakly similar to many e.g. TR:AAD32748 (EMBL:AF127374) transcriptional repressor from Streptomyces lavendulae (163 aa) fasta scores; opt: 142, z-score: 184.9, E(): 0.0062, (27.3% identity in 128 aa overlap). Contains Pfam match to entry PF01047 MarR, MarR family and probable helix-turn-helix motif (+3.47 SD) 68-89aa. 4 1 13 6 [Reporter]SCO3352 (16D24)_PCR/1781 [Gene]SCO3352/1631 NC_003888 SCE94.03, possible DNA-binding protein, len: 374 aa; similar to hypothetical proteins eg. TR:O53571 (EMBL:AL022075) from Mycobacterium tuberculosis (358 aa) fasta scores; opt: 1338, z-score: 1480.9, E(): 0, (59.9% identity in 347 aa overlap). Contains Pfam match to entry PF00633 HHH, Helix-hairpin-helix motif. 4 1 13 5 [Reporter]SCO0834 (17P20)_PCR/1780 [Gene]SCO0834/1630 NC_003888 SCF43A.24c, unknown, len: 62 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. An alternative start codon is present at codon 7, but without an apparent RBS 1 3 8 2 [Reporter]SCO3572 (20I10)_PCR/4187 [Gene]SCO3572/3809 NC_003888 SCH17.06c, possible hydrolase, len: 256 aa; weakly similar to eukaryotic glyoxalase e.g. SW:GL2M_ARATH (EMBL:U90928), glx2-1, Arabidopsis thaliana hydroxyacylglutathione hydrolase (331 aa), fasta scores; opt: 186 z-score: 218.1 E(): 8e-05, 29.2% identity in 161 aa overlap. Similar to many hypothetical proteins e.g. TR:O69645 (EMBL:AL022121) Mycobacterium tuberculosis putative hydrolase (264 aa) (54.7% identity in 247 aa overlap) 1 3 7 22 [Reporter]SCO4213 (1I6)_PCR/4185 [Gene]SCO4213/3808 NC_003888 2SCD46.27, unknown, len: 544 aa; C-terminal region similar to that of SW:P24202 (MRR_ECOLI) Mrr restriction system protein from Escherichia coli (304 aa) fasta scores; opt: 199, z-score: 216.8, E(): 0.00013, 28.4% identity in 162 aa overlap. N-terminal region contains possible coiled-coil sequences residues (33-63 and 156-200) and two TTA encoded leucine residues which may be a target for bldA regulation. 1 3 7 21 [Reporter]SCO2431 (2E6)_PCR/4184 [Gene]SCO2431/3807 NC_003888 SCC24.02c, abfA, alpha-L-arabinofuranosidase, len: 501 aa; almost identical to SW:ABFA_STRLI (EMBL:U04630) Streptomyces lividans alpha-L-arabinofuranosidase (EC 3.2.1.55) AbfA, 662 aa 1 3 7 20 [Reporter]SCO4138 (3A6)_PCR/4183 [Gene]SCO4138/3806 NC_003888 SCD84.05, pitH, phosphate transport protein, len: 332 aa; similar to N-terminal region of SW:PITH_STRHA (EMBL:L05390) Streptomyces halstedii putative low-affinity inorganic phosphate transporter (fragment), 213 aa; fasta scores: opt: 768 z-score: 850.1 E(): 0; 59.8% identity in 189 aa overlap, to TR:O30499 (EMBL:AF008187) Sinorhizobium meliloti phosphate transport protein, Pit, 334 aa; fasta scores: opt: 988 z-score: 1087.8 E(): 0; 48.3% identity in 327 aa overlap and to TR:CAB59459 (EMBL:AL132644) Streptomyces coelicolor putative secreted protein SCI8.28, 423 aa; fasta scores: opt: 991 z-score: 986.9 E(): 0; 45.9% identity in 331 aa overlap. Contains Pfam match to entry PF01384 PHO4, Phosphate transporter family. Contains also possible hydrophobic membrane spanning regions 1 3 7 19 [Reporter]SCO7202 (4M2)_PCR/4182 [Gene]SCO7202/3805 NC_003888 SC2H12.01, possible membrane protein, partial CDS, len: >87 aa. Contains a possible membrane spanning hydrophobic domain.,SC1D2.05, possible membrane protein, partial CDS, len >191 aa. Contains possible membrane spanning hydrophobic domains. Overlaps and extends into CDS SC2H12.01 on the adjoining cosmid. 1 3 7 18 [Reporter]SCO6843 (5I2)_PCR/4181 [Gene]SCO6843/3804 NC_003888 SC3D9.11c, unknown, len: 372 aa. Weakly similar to Streptomyces coelicolor TR:Q9X8A4 (EMBL:AL049645) hypothetical protein, SCE2.09 (386 aa), fasta scores opt: 559 z-score: 616.2 E(): 6.5e-27; 37.5% identity in 363 aa overlap 1 3 7 17 [Reporter]SCO6628 (6E2)_PCR/4180 [Gene]SCO6628/3803 NC_003888 SC4G2.02c, unknown, len: 196 aa; similar to S. coelicolor TR:Q53897 (EMBL:X60316) abaA gene ORFA (192 aa), fasta scores; opt: 245 z-score: 250.4 E(): 1.1e-06, 32.9% identity in 164 aa overlap. Also similar to SC10A5.20 (32.7% identity in 147 aa overlap) and SC7C7.02c (38.7% identity in 150 aa overlap) and to TR:Q9RKV5 (EMBL:AL132824) Streptomyces coelicolor putative regulatory protein SCAH10.02, 160 aa; fasta scores: opt: 436 Z-score: 526.8 E(): 1e-21; 51.449% identity in 138 aa overlap 4 1 13 4 [Reporter]SCP1.225 (18L20)_PCR/1779 [Gene]SCP1.225/1629 NC_003888 SCP1.225, unknown, len: 117aa; previously partially sequenced and annotated as TR:Q9JN71 (EMBL:AJ276673). Note possible alternative downstream translational start sites. 1 3 7 16 [Reporter]SCO0301 (7A2)_PCR/4179 [Gene]SCO0301/3802 NC_003888 SC5G9.10, possible lipoprotein, len: 342 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains a probable N-terminal signal sequence and an appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 4 1 13 3 [Reporter]SCO6276 (19H20)_PCR/1778 [Gene]SCO6276/1628 NC_003888 SC1G7.02, possible secreted protein, len: 440 aa; similar to TR:Q9L0A8 (EMBL:AL163003) Streptomyces coelicolor putative secreted protein SCC24.09, 468 aa; fasta scores: opt: 777 Z-score: 867.4 bits: 169.7 E(): 1e-40; 36.303% identity in 449 aa overlap. Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase 1 3 7 15 [Reporter]SCO6964 (7M22)_PCR/4178 [Gene]SCO6964/3801 NC_003888 SC6F7.17c, unknown, len: 216 aa. Similar to Mycobacterium tuberculosis TR:P95145(EMBL:Z83859) hypothetical 23.1 kd protein (211 aa), fasta scores opt: 519 z-score: 613.8 E(): 9.4e-27 46.6% identity in 193 aa overlap 4 1 13 2 [Reporter]SCO5073 (20P16)_PCR/1777 [Gene]SCO5073/1627 NC_003888 SCBAC20F6.16, ORF2, possible oxidoreductase, len: 329 aa; identical to previously sequenced TR:Q53927 (EMBL:X62373) Streptomyces coelicolor genes for hydroxylacyl-CoA dehydrogenase ORF2, 329 aa. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and match to Prosite entry PS01162 Quinone oxidoreductase / zeta-crystallin signature 1 3 7 13 [Reporter]SCO6059 (9E22)_PCR/4176 [Gene]SCO6059/3800 NC_003888 SC9B1.06, unknown, len: 181 aa 4 1 12 22 [Reporter]SCO1277 (1P12)_PCR/1775 [Gene]SCO1277/1626 NC_003888 2SCG18.24, unknown, len: 247 aa; identical to N-terminal region of TR:AAF35372 (EMBL:AF225460) Streptomyces coelicolor hypothetical 7.4 kD protein (fragment), 72 aa; fasta scores: opt: 452 z-score: 395.3 E(): 1.6e-14; 94.4% identity in 72 aa overlap. Contains 4 sets of different repeats: 2x conserved repeat: SGSSR, 6x degenerate repeat: NPLA(A/G), 7x degenerate repeat: G(A/G)A(G/Q/N) and 7x degenerate repeat: Q(G/V/L)(L/T) 4 1 12 21 [Reporter]SCO5586 (2L12)_PCR/1774 [Gene]SCO5586/1625 NC_003888 SC2E1.03, ffh, signal recognition particle protein, len: 550 aa; highly similar to mnay e.g. SR54_ECOLI (453 aa), fatsa scores; opt: 1465 z-score: 1282.8 E(): 0, 50.0% identity in 458 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00300 SRP54-type proteins GTP-binding domain signature and Pfam match to entry SRP54 PF00448, SRP54-type proteins, score 66.57. Similarities suggest possible start site at aa 35 4 1 12 20 [Reporter]SCO5882 (3H12)_PCR/1773 [Gene]SCO5882/1624 NC_003888 SC3F7.02c, redV, function unknown, len: 395 aa 4 1 12 19 [Reporter]SCO6026 (4D12)_PCR/1772 [Gene]SCO6026/1623 NC_003888 SC1C3.14c, probable fatty acid oxidation complex alpha-subunit, similar to many e.g. FADB_ECOLI (729 aa), fasta scores; opt: 782 z-score: 863.1 E(): 0, 29.8% identity in671 aa overlap. Contains Pfam match to entry ECH PF00378, Enoyl-CoA hydratase/isomerase, score 49.78 4 1 12 18 [Reporter]SCO2080 (5P8)_PCR/1771 [Gene]SCO2080/1622 NC_003888 SC4A10.13c, conserved hypothetical protein, len: 239 aa; unknown function, similar to many hypothetical proteins e.g. SW:YPT5_PSEAE (EMBL:M55524) Pseudomonas aeruginosa hypothetical protein (230 aa), fasta scores; opt: 464 z-score: 546.4 E(): 4.1e-23, 39.7% identity in 229 aa overlap. Contains Pfam match to entry PF01168 UPF0001, Uncharacterized protein family UPF0001 and PS01211 Uncharacterized protein family UPF0001 signature 4 1 12 17 [Reporter]SCO7449 (6L8)_PCR/1770 [Gene]SCO7449/1621 NC_003888 SC5C11.06, possible secreted/membrane protein, len: 225 aa. Contains repeat sequences 3xGDW(N/D)HDD(S/T/A)S(Y/S)(S/G)KEHDNGSKHDEPRGG, 3xMHTGGG(A/G)LAV(T/N)E and 1xMHTGGGGLA. Also contains possible N-terminal region signal sequence peptide and possible membrane spanning hydrophobic domains. 4 1 12 16 [Reporter]SCO7253 (7H8)_PCR/1769 [Gene]SCO7253/1620 NC_003888 SC5H1.39, hypothetical protein, len: 438 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 4 1 12 15 [Reporter]SCO5329 (8D8)_PCR/1768 [Gene]SCO5329/1619 NC_003888 SC6G9.04c, hypothetical protein, len: 565 aa; similar to TR:O34810 (EMBL:AE000641) Helicobacter pylori hypothetical protein (578 aa), fasta scores; opt: 391 z-score: 464.1 E(): 1.6e-18, 23.6% identity in 525 aa overlap and to several other hypothetical proteins from the same organism, with the highest similarity toward the N-terminus. The coding sequence has unusually low %G+C content 4 1 12 14 [Reporter]SCO7821 (9P4)_PCR/1767 [Gene]SCO7821/1618 NC_003888 SC8E7.18, possible integral membrane protein, len: 137 aa. Similar in parts to other Streptomyces coelicolor membrane proteins e.g. TR:O86735(EMBL:AL031035) SC3A9.03C (149 aa), fasta scores opt: 148 z-score: 187.6 E(): 0.0056 35.9% identity in 103 aa overlap. Contains possible membrane spanning hydrophobic domains. 4 1 12 13 [Reporter]SCO2780 (10L4)_PCR/1766 [Gene]SCO2780/1617 NC_003888 SCC105.11, possible secreted protein, len: 349 aa; similar to TR:CAB53324 (EMBL:AL109974) Streptomyces coelicolor putative iron-siderophore binding lipoprotein SCF34.13c, 350 aa; fasta scores: opt: 288 z-score: 297.2 E(): 4.1e-09; 28.3% identity in 332 aa overlap. Contains possible N-terminal region signal peptide sequence 4 1 12 12 [Reporter]SCO2274 (11H4)_PCR/1765 [Gene]SCO2274/1616 NC_003888 SCC75A.20, ABC transport protein, ATP-binding component, len: 280 aa. Highly similar to many other membrane transport proteins involved in iron acquisition including: Yersinia pestis SW:HMUV_YERPE(EMBL:U60647) hemin transport system ATP-binding protein HmuV (266 aa), fasta scores opt: 609 z-score: 603.6 E(): 3e-26 43.0% identity in 251 aa overlap and Corynebacterium diphtheriae TR:AAD37495(EMBL:AF109162) ATP-binding protein HmuV (277 aa), fasta scores opt: 695 z-score: 686.7 E(): 7e-31 49.0% identity in 241 aa overlap. Contains a Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite hits to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. 4 1 12 11 [Reporter]SCO4787 (12D4)_PCR/1764 [Gene]SCO4787/1615 NC_003888 SCD63.19, possible aldolase, len: 267 aa; similar to SW:ADDA_HUMAN (EMBL:X58141) Homo sapiens erythrocyte adducin alpha subunit AddA, 737 aa; fasta scores: opt: 422 z-score: 487.2 E(): 1e-19; 35.4% identity in 240 aa overlap . Contains Pfam match to entry PF00596 Aldolase_II, Class II Aldolase and Adducin N-terminal domain 4 1 12 10 [Reporter]SCO2849 (12P24)_PCR/1763 [Gene]SCO2849/1614 NC_003888 SCE20.23, unknown, len: 290 aa. Similar to a protein of undefined function from Mycobacterium tuberculosis TR:O06328(EMBL:Z95390) hypothetical 30.7 KD protein (285 aa), fasta scores opt: 770 z-score: 928.4 E(): 0 48.6% identity in 247 aa overlap. 4 1 12 9 [Reporter]SCO1828 (13L24)_PCR/1762 [Gene]SCO1828/1613 NC_003888 SCI8.13, unknown, len: 77 aa. 4 1 12 8 [Reporter]SCO3809 (14H24)_PCR/1761 [Gene]SCO3809/1612 NC_003888 SCGD3.10c, probable transmembrane transport protein, len: 436 aa; similar to several hypothetical transporters and weakly similar to TR:O54041 (EMBL:Y15252), narK1, Pseudomonas aeruginosa nitrate extrusion protein (341 aa), fasta scores; opt: 243 z-score: 275.0 E(): 5.5e-08, 24.9% identity in 422 aa overlap. Weakly similar to many from S.coelicolor e.g. (EMBL:AL034443), SC4B5.13, probable integral membrane transporter (446 aa) (24.6% identity in 345 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter 4 1 12 7 [Reporter]SCO1535 (15D24)_PCR/1760 [Gene]SCO1535/1611 NC_003888 SCL2.25c, unknown, len: 152 aa 4 1 12 6 [Reporter]SCO0674 (16P20)_PCR/1759 [Gene]SCO0674/1610 NC_003888 SCF91.34c, xysA, secreted endo-1,4-beta-xylanase (EC 3.2.1.8), len: 450 aa. Highly similar to many including: Streptomyces lividans SW:XYNA_STRLI(EMBL:M64551) endo-1,4-beta-xylanase A precursor (EC 3.2.1.8) (477 aa),fasta scores opt: 1313 z-score: 1280.4 E(): 0 49.0% identity in 459 aa overlap and Streptomyces halstedii TR:Q59922(EMBL:U41627) endo-1,4-beta-xylanase (EC 3.2.1.8) (461 aa), fasta scores opt: 2301 z-score: 2238.0 E():0 76.9% identity in 438 aa overlap. Contains Pfam matches to entries PF00553 CBD_2, Cellulose binding domain and Pfam match to entry PF00331 Glyco_hydro_10, Glycosyl hydrolase family 10. Also contains a Prosite hit to PS00591 Glycosyl hydrolases family 10 active site and a possible N-terminal signal sequence. 4 1 12 5 [Reporter]SCO3705 (17L20)_PCR/1758 [Gene]SCO3705/1609 NC_003888 SCH35.19c, ABC transporter membrane subunit, len: 283aa; similar to many egs. TR:O31230 (EMBL:Y10817) from Arthrobacter nicotinovorans (239 aa) fasta scores; opt: 745, z-score: 854.5, E(): 0, (52.8% identity in 229 aa overlap) and SW:MODB_ECOLI molybdate transport permease from Escherichia coli (229 aa) fasta scores; opt: 639, z-score: 734.6, E(): 0, (44.7% identity in 219 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains possible membrane spanning hydrophobic regions. 4 1 12 4 [Reporter]SCO1043 (18H20)_PCR/1757 [Gene]SCO1043/1608 NC_003888 SCG20A.23, probable transcriptional regulatory protein, len: 259 aa; similar to SW:SRLR_ECOLI (EMBL:J02708) Escherichia coli glucitol operon repressor SrlR, 257 aa; fasta scores: opt: 429 z-score: 487.9 E(): 1.1e-19; 30.6% identity in 258 aa overlap. Contains Pfam match to entry PF00455 deoR, Bacterial regulatory proteins, deoR family and match to Prosite entry PS00894 Bacterial regulatory proteins, deoR family signature. Contains also a possible helix-turn-helix motif at residues 20..41 (+2.55 SD) 4 1 12 3 [Reporter]SCO1978 (19D20)_PCR/1756 [Gene]SCO1978/1607 NC_003888 SC3C9.13c, possible AbaA-like protein, len: 91 aa; similar to TR:Q9F3E1 (EMBL:AL450289) Streptomyces coelicolor putative AbaA-like protein SC2H2.14, 89 aa; fasta scores: opt: 371 Z-score: 479.1 bits: 93.2 E(): 4.5e-19; 69.863% identity in 73 aa overlap and to TR:Q53896 (EMBL:X60316) Streptomyces coelicolor AbaA gene (putative regulator) SCF42.13, 75 aa; fasta scores: opt: 220 Z-score: 293.4 bits: 58.6 E(): 9.9e-09; 50.000% identity in 74 aa overlap 4 1 12 2 [Reporter]SCO3967 (20L16)_PCR/1755 [Gene]SCO3967/1606 NC_003888 SCBAC25E3.04c, conserved hypothetical membrane protein, len: 253aa: similar to many eg. TR:Q9RPV8 (EMBL:AF142677) hypothetical protein from Bacillus megaterium plasmid pBM500 (211 aa) fasta scores; opt: 205, Z-score: 224.5, 28.745% identity (34.135% ungapped) in 247 aa overlap. Contains possible membrane-spanning hydrophobic regions. 4 1 11 22 [Reporter]SCO1272 (1L12)_PCR/1753 [Gene]SCO1272/1605 NC_003888 2SCG18.19c, probable acyl carrier protein, len: 90 aa; similar to SW:ACPA_STRCO (EMBL:X63449) Streptomyces coelicolor actinorhodin polyketide synthase acyl carrier protein (ActI-ORF3), 86 aa; fasta scores: opt: 189 z-score: 253.9 E(): 1.2e-06; 40.2% identity in 82 aa overlap. Contains Pfam match to entry PF00550 pp-binding, Phosphopantetheine attachment site and match to Prosite entry PS00012 Phosphopantetheine attachment site 4 1 11 21 [Reporter]SCO3795 (2H12)_PCR/1752 [Gene]SCO3795/1604 NC_003888 SCAC2.03c, aspS, aspartyl-tRNA synthetase, len: 587 aa; similar to SW:SYD_ECOLI (EMBL:X53863) Escherichia coli aspartyl-tRNA synthetase (EC 6.1.1.12) AspS, 590 aa; fasta scores: opt: 1514 z-score: 1745.0 E(): 0; 42.0% identity in 590 aa overlap. Contains Pfam match to entry PF01336 tRNA_anti, tRNA synthetase anti-codon binding domain and PF00152 tRNA-synt_2, tRNA synthetases class II (D, K and N) 4 1 11 19 [Reporter]SCO6935 (4P8)_PCR/1750 [Gene]SCO6935/1603 NC_003888 SC1G8.07c, unknown, len: 191 aa. 4 1 11 18 [Reporter]SCO2076 (5L8)_PCR/1749 [Gene]SCO2076/1602 NC_003888 SC4A10.09, probable isoleucyl-tRNA synthetase, len: 1047 aa; similar to many e.g. SW:SYIP_STAAU (EMBL:X75439), mupR, Staphylococcus aureus isoleucyl-tRNA synthetase conferring mupirocin resistance (1024 aa), fasta scores; opt: 2453 z-score: 2768.9 E(): 0, 38.2% identity in 985 aa overlap and SW:SYI_THETH, ileS, Thermus aquaticus isoleucyl-tRNA synthetase (821 aa) (49.1% identity in 831 aa overlap). Contains Pfam match to entry PF00133 tRNA-synt_1, tRNA synthetases class I (I, L, M and V) 4 1 11 17 [Reporter]SCO2064 (6H8)_PCR/1748 [Gene]SCO2064/1601 NC_003888 SC4G6.33c, dnaE, probable DNA polymerase III alpha chain, len: 1179 aa; similar to many e.g. SW:DP3A_ECOLI DnaE, DNA polymerase III alpha chain (1160 aa) fasta scores; opt: 2252, z-score: 2561.2, E(): 0, (36.1% identity in 1193 aa overlap). 4 1 11 14 [Reporter]SCO7817 (9L4)_PCR/1745 [Gene]SCO7817/1600 NC_003888 SC8E7.14, possible DNA-binding protein, len: 280 aa. Highly similar to many other putative DNA-binding proteins e.g. Streptomyces coelicolor TR:Q9S1Q8 (EMBL:AL109972) SCJ9A.12C (279 aa), fasta scores opt: 946 z-score: 1117.2 E(): 0 54.1% identity in 268 aa overlap. Contains a putative helix-turn-helix motif situated between residues 131..153 (+3.68 SD) 1 1 5 21 [Reporter]SCO1187 (2M4)_PCR/108 [Gene]SCO1187/99 NC_003888 SCG11A.18, celB, secreted cellulase B precursor, len: 381 aa; identical to TR:Q54331 (EMBL:U04629) Streptomyces lividans cellulase precursor, celB, 381 aa and similar to SW:GUX_CELFI (EMBL:M15824) Cellulomonas fimi exoglucanase/xylanase precursor [includes: exoglucanase (EC 3.2.1.91) (exocellobiohydrolase) (1,4-beta-cellobiohydrolase) (beta-1,4- glycanase Cex); endo-1,4-beta-xylanase B (EC 3.2.1.8) (xylanase B) Cex or XynB, 484 aa; fasta scores: opt: 422 z-score: 450.5 E(): 1e-17; 48.8% identity in 123 aa overlap. Contains two matches to Pfam entries PF01670 Glyco_hydro_12, Glycosyl hydrolase family 12 and PF00553 CBD_2, Cellulose binding domain and match to Prosite entry PS00561 Cellulose-binding domain, bacterial type. Contains also possible signal peptide sequence 1 1 5 20 [Reporter]SCO4721 (3I4)_PCR/107 [Gene]SCO4721/98 NC_003888 SCD31.46, rplO, 50S ribosomal protein L15, len: 151 aa; identical to previously sequenced SW:RL15_STRCO (EMBL:X83011) Streptomyces coelicolor 50S ribosomal protein L15 RplO, 151 aa. Contains Pfam matches to entries PF01305 Ribosomal_L15, Ribosomal protein L15 amino terminal region and fam match to entry PF00256 L15, Ribosomal protein L15 and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00475 Ribosomal protein L15 signature 1 1 5 19 [Reporter]SCO6915 (4E4)_PCR/106 [Gene]SCO6915/97 NC_003888 SC1B2.21, unknown, len: 273 aa. 1 1 5 18 [Reporter]SCO6353 (5A4)_PCR/105 [Gene]SCO6353/96 NC_003888 SC3A7.21c, probable two-component sensor, len: 459 aa; similar to many e.g. COPS_PSESM sensor protein copS (EC 2.7.3.-) (487 aa), fasta scores; opt: 310 z-score: 293.0 E(): 4.6e-09, 24.2% identity in 467 aa overlap. Contains a possible transmembrane domain near the N-terminus and Pfam match to entry PF00512 signal, Signal carboxyl-terminal domain, score 113.70, E-value 3.5e-30 1 1 5 17 [Reporter]SCO7324 (5M24)_PCR/104 [Gene]SCO7324/95 NC_003888 SC4G10.03c, possible regulatory protein, len: 299 aa. Similar to Bacillus subtilis SW:RSBR_BACSU(EMBL:L35574) positive regulator of sigma-B activity, RsbR (274 aa), fasta scores opt: 401 z-score: 450.7 E(): 1.2e-17 27.6% identity in 279 aa overlap. 1 1 5 16 [Reporter]SCO4850 (6I24)_PCR/103 [Gene]SCO4850/94 NC_003888 SC5G8.18c, possible tetR-family transcriptional regulator, len: 251 aa; N-terminal region similar to many e.g. TR:CAB53315 (EMBL:AL109974) putative tetR-family transcriptional regulator from Streptomyces coelicolor (203 aa) fasta scores; opt: 190, z-score: 242.1, E(): 5e-06, 35.0% identity in 103 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and helix-turn-helix motif 40..61 aa 1 1 5 15 [Reporter]SCO1432 (7E24)_PCR/102 [Gene]SCO1432/93 NC_003888 SC6D7.07, possible membrane protein, len: 479 aa. Contains a possible membrane spanning hydrophobic domain. 1 1 5 14 [Reporter]SCO5947 (8A24)_PCR/101 [Gene]SCO5947/92 NC_003888 SC7H1.17, probable integral membrane protein, len: 465 aa; weakly similar to many eg. Mycobacterium tuberculosis TR:O07753 (EMBL:Z97193) MTCY180.41C (687 aa), fasta scores; opt: 440 z-score: 332.4 E(): 2.5e-11, 26.6% identity in 413 aa overlap. Contains possible hydrophobc membrane spanning regions 1 1 5 13 [Reporter]SCO6456 (9M20)_PCR/100 [Gene]SCO6456/91 NC_003888 SC9B5.23c, probable hydrolytic protein, len: 464aa; similar to many eg. SW:ABFB_STRLI alpha-l-arabinofuranosidase precursor from Streptomyces lividans (478 aa) fasta scores: opt: 323, z-score: 291.1, E(): 6.4e-09, (37.9% identity in 132 aa overlap). Contains Pfam match to entry PF00652 Ricin_B_lectin, Similarity to lectin domain of ricin beta-chain. Contains possible hydrophobic membrane spanning region 1 1 5 11 [Reporter]SCO4598 (11E20)_PCR/98 [Gene]SCO4598/90 NC_003888 SCD20.16c, possible two-component system sensor kinase, len: 407 aa; similar to TR:CAB81858 (EMBL:AL161691) Streptomyces coelicolor putative two-component sensor kinase SCD40A.13, 431 aa; fasta scores: opt: 659 z-score: 729.4 E(): 0; 37.0% identity in 427 aa overlap and to Streptomyces coelicolor putative two-component system sensor kinase SCD20.15c, 455 aa; fasta scores: opt: 1179 z-score: 1124.4 E(): 0; 57.2% identity in 442 aa overlap. Contains possible hydrophobic membrane spanning regions 1 1 5 10 [Reporter]SCO4302 (12A20)_PCR/97 [Gene]SCO4302/89 NC_003888 SCD95A.35, possible secreted protein, len: 129 aa; N-terminal domain similar to TR:CAB69733 (EMBL:AL137166) Streptomyces coelicolor hypothetical 7.1 kD protein SCC121.15c, 68 aa; fasta scores: opt: 202 z-score: 262.4 E(): 3.7e-07; 52.5% identity in 59 aa overlap and C-terminal domain similar to TR:CAB69732 (EMBL:AL137166) Streptomyces coelicolor hypothetical 7.8 kD protein SCC121.14c, 73 aa; fasta scores: opt: 198 z-score: 257.2 E(): 7.3e-07; 51.6% identity in 64 aa overlap. Contains possible N-terminal region signal peptide sequence 1 1 5 9 [Reporter]SCO3779 (13M16)_PCR/96 [Gene]SCO3779/88 NC_003888 SCH63.26, probable nucleoside hydrolase, len: 326 aa; similar to SW:IUNH_CRIFA (EMBL:U43371) Crithidia fasciculata inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1) IunH, 314 aa; fasta scores: opt: 634 z-score: 671.5 E(): 6.9e-30; 35.0% identity in 320 aa overlap. Contains Pfam match to entry PF01156 IU_nuc_hydro, Inosine-uridine preferring nucleoside hydrolase 1 1 5 8 [Reporter]SCO1785 (14I16)_PCR/95 [Gene]SCO1785/87 NC_003888 SCI51.25c, probable iron-siderophore uptake system ATP-binding component, len: 301 aa; similar to many ATP-binding components from binding-protein-dependent iron uptake systems using a range of siderophores e.g. SW:FEPC_ECOLI (EMBL:X57471), fepC, Escherichia coli ferric enterobactin transport ATP-binding protein (271 aa), fasta scores; opt: 1101 z-score: 1184.4 E(): 0, 61.5% identity in 260 aa overlap. Similar to other binding-protein-dependent permease subunits e.g. SC9B1.09 (EMBL:AL049727) S.coelicolor putative ABC transporter ATP-binding subunit (386 aa) (37.7% identity in 228 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 1 1 5 7 [Reporter]SCO3395 (15E16)_PCR/94 [Gene]SCO3395/86 NC_003888 SCE9.02c, putative secreted protein, len: 391 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Hydrophobic region at N-terminus and degenerate E(T/A)(P/R)EQ repeat in centre. Contains probable coiled-coil from 45 to 114 (70 residues) (Max score: 1.834, probability 1.00). Contains possible N-terminal region signal peptide sequence 1 1 5 6 [Reporter]SCO0031 (16A16)_PCR/93 [Gene]SCO0031/85 NC_003888 SCJ4.12c, unknown, len: 180 aa 1 1 5 5 [Reporter]SCO4964 (17M12)_PCR/92 [Gene]SCO4964/84 NC_003888 2SCK31.24, probable integral membrane transport protein, len: 285 aa; similar to TR:AAG32069 (EMBL:AF317791) Streptomyces viridochromogenes hydrophobic transmembrane protein AviABCII, 265 aa; fasta scores: opt: 677 z-score: 786.2 E(): 0; 39.7% identity in 262 aa overlap. Contains possible hydrophobic membrane spanning regions 1 1 5 4 [Reporter]SCO3808 (18I12)_PCR/91 [Gene]SCO3808/83 NC_003888 SCGD3.09, hypothetical protein, len: 274 aa; unknown function, similar to TR:O05777 (EMBL:Z95150) Mycobacterium tuberculosis hypothetical protein (283 aa), fasta scores; opt: 384 z-score: 466.0 E(): 1.3e-18, 32.7% identity in 269 aa overlap 1 1 5 3 [Reporter]SCP1.283 (19E12)_PCR/90 [Gene]SCP1.283/82 NC_003888 SCP1.283, doubtful CDS, unknown, len: 54aa; 1 1 4 22 [Reporter]SCO4199 (1M4)_PCR/87 [Gene]SCO4199/81 NC_003888 2SCD46.13, unknown, len: 213aa; contains mostly hydrophilic residues. 1 1 4 21 [Reporter]SCO5960 (2I4)_PCR/86 [Gene]SCO5960/80 NC_003888 SC7H1.30c, cbiN, probable cobalt transport protein, len: 118 aa; similar to many eg. CBIN_SALTY Q05595 cobalt transport protein cbin (93 aa), fasta scores; opt: 283 z-score: 433.7 E(): 5.8e-17, 47.3% identity in 93 aa overlap. Contains possible N-terminal signal sequence 3 4 4 8 [Reporter]SCO0160 (14G13)_PCR/7128 [Gene]SCO0160/6499 NC_003888 SCJ1.09c, possible DNA-binding protein, len: 195 aa, similarity to Mycobacterium tuberculosis TR:O53625 (EMBL; AL021428) hypothetical 16.6 KD protein (152 aa), fasta scores opt: 182 z-score: 220.8 E(): 6.1e-05 34.2% identity in 155 aa overlap and Mycobacterium tuberculosis TR:O05803 (EMBL; Z95150) hypothetical 12.3 KD protein (110 aa), fasta scores opt: 142 z-score: 177.2 E(): 0.016 34.8% identity in 89 aa overlap. Contains a Pfam match to entry PF01381 HTH_3, Helix-turn-helix and a possible helix-turn-helix motif at residues 34..55 (+3.91 SD) 3 4 4 7 [Reporter]SCO0959 (15C13)_PCR/7127 [Gene]SCO0959/6498 NC_003888 SCM11.14c, possible secreted protein, len: 772 aa; similar to N-terminus of TR:O69822 (EMBL:AL023496) Streptomyces coelicolor putative secreted protein SC1A6.17c, 2314 aa; fasta scores: opt: 444 z-score: 500.2 E(): 1.8e-20; 30.0% identity in 818 aa overlap and low similarities to TR: (EMBL:) Pseudomonas sp. acetaldehyde dehydrigenase (acylating) PhnI, 312 aa; fasta scores: opt: 125 z-score: 146.1 E(): 0.92; 23.7% identity in 177 aa overlap . Contains possible signal peptide sequence 3 4 4 6 [Reporter]SCO1529 (16O9)_PCR/7126 [Gene]SCO1529/6497 NC_003888 SCL2.19, possible membrane protein, len: 551 aa. Contains possible hydrophobic membrane spanning regions 3 4 4 5 [Reporter]SCO1819 (17K9)_PCR/7125 [Gene]SCO1819/6496 NC_003888 SCI8.04c, possible integral membrane protein, len: 93 aa. Similar to Streptomyces coelicolor TR:CAB42957 (EMBL: AL049863) possible membrane protein SC5H1.32 (89 aa), fasta scores opt: 180 z-score: 240.4 E(): 5.1e-06 34.2% identity in 76 aa overlap and Escherichia coli SW:TAG1_ECOLI (EMBL: U85771) transglycosylase associated protein (84 aa), fasta scores opt: 166 z-score: 223.5 E(): 4.4e-05 36.4% identity in 77 aa overlap. Contains a possible membrane spanning hydrophobic domains. 1 1 4 20 [Reporter]SCO2643 (3E4)_PCR/85 [Gene]SCO2643/79 NC_003888 SC8E4A.13, pepN, aminopeptidase N, len: 857 aa; identical to SW:AMPN_STRLI (EMBL:L23172) Streptomyces lividans aminopeptidase N (EC 3.4.11.2) PepN, 857 aa and similar to SW:AMPN_LACLA (EMBL:D38040) Lactococcus lactis aminopeptidase N (EC 3.4.11.2) PepN, 848 aa; fasta scores: opt: 738 z-score: 846.7 E(): 0; 33.4% identity in 440 aa overlap and to S. coelicolor SC8E4A.05, 833 aa; fasta scores: opt: 2998 z-score: 2943.4 E(): 0; 55.8% identity in 842 aa overlap. Contains Pfam match to entry PF01433 Peptidase_M1, Peptidase family M1 and match ot Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature 3 4 4 4 [Reporter]SCO1558 (18G9)_PCR/7124 [Gene]SCO1558/6495 NC_003888 SCL11.14c, probable ABC transporter permease protein, len: 240 aa; similar to TR:AAF10929 (EMBL:AE001982) Deinococcus radiodurans ABC transporter, permease protein DR1357, 218 aa; fasta scores: opt: 721 z-score: 842.8 E(): 0; 57.7% identity in 213 aa overlap and to SW:PROW_BACSU (EMBL:U38418) Bacillus subtilis glycine betaine/L-proline transport system permease protein ProW, 217 aa; fasta scores: opt: 212 z-score: 255.8 E(): 7.9e-07; 28.4% identity in 194 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component 1 1 4 19 [Reporter]SCO6908 (4A4)_PCR/84 [Gene]SCO6908/78 NC_003888 SC1B2.14c, unknown, len: 434 aa. 3 4 4 3 [Reporter]SCO4028 (19C9)_PCR/7123 [Gene]SCO4028/6494 NC_003888 2SC10A7.32, hypothetical protein, len: 273 aa; similar to TR:CAB76342 (EMBL:AL158061) Streptomyces coelicolor hypothetical 26.1 kDa protein SC6D11.19c, 236 aa; fasta scores: opt: 291 z-score: 349.8 E(): 5.6e-12; 33.0% identity in 221 aa overlap. Contains Pfam match to entry PF01590 GAF, GAF domain 1 1 4 18 [Reporter]SCO7088 (4M24)_PCR/83 [Gene]SCO7088/77 NC_003888 SC3A4.14c, two-component system response regulator, len: 225 aa. Similar to Escherichia coli SW:NARL_ECOLI(EMBL:X13360) nitrate/nitrite response regulator protein, NarL (216 aa), fasta scores opt: 375 z-score:0.0 E(): 0.0 33.79% identity in 219 aa overlap. Also highly similar to multiple Streptomyces coelicolor putative two-component response regulators e.g. TR:CAB59718(EMBL:AL132707) SCF51.20C (219 aa), fasta scores opt: 933 z-score: 1109.1 E():0 73.3% identity in 217 aa overlap. Contains a Prosite hits to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00622 Bacterial regulatory proteins, luxR family signature. Also contains Pfam matches to entries PF00196 GerE, Bacterial regulatory proteins, luxR family and PF00072 response_reg, Response regulator receiver domain with the putative helix-turn-helix motif situated between residues 174..195 (+3.75 SD). 3 4 4 2 [Reporter]SCO5348 (20K5)_PCR/7122 [Gene]SCO5348/6493 NC_003888 SCBAC5H2.17, possible excisionase, len: 61 aa; similar to TR:Q53965 (EMBL:X71358) Streptomyces coelicolor plasmid SLP1 DNA for xis and int genes, Xis, 61 aa; fasta scores: opt: 148 z-score: 233.1 E(): 2.1e-05; 38.3% identity in 60 aa overlap 1 1 4 17 [Reporter]SCO7073 (5I24)_PCR/82 [Gene]SCO7073/76 NC_003888 SC4G1.39c, hypothetical protein, len: 330 aa; similar to SW:YCGT_ECOLI (EMBL:AE000218) Escherichia coli hypothetical 39.5 kDa protein in TreA-Pth intergenic region YcgT, 366 aa; fasta scores: opt: 876 z-score: 955.5 E(): 0; 46.2% identity in 355 aa overlap, to N-terminal region of SW:DAK1_YEAST (EMBL:Z38114) Saccharomyces cerevisiae dihydroxyacetone kinase 1 (EC 2.7.1.29) Dak1, 584 aa; fasta scores: opt: 516 z-score: 562.8 E(): 7e-24; 34.0% identity in 312 aa overlap and to TR:AAK84068 (EMBL:AF297121) Selenomonas ruminantium subsp. ruminantium dihydroxyacetone kinase DhaK1, 329 aa; fasta scores: opt: 1017 Z-score: 1086.8 E(): 6.7e-53; 49.848% identity in 329 aa overlap 3 4 3 22 [Reporter]SCO4534 (1K1)_PCR/7120 [Gene]SCO4534/6492 NC_003888 2SCD4.05, possible membrane protein, len: 474 aa; similar to TR:CAB77306 (EMBL:AL160312) Streptomyces coelicolor hypothetical 42.7 kDa protein SCD35.22c, 401 aa; fasta scores: opt: 583 z-score: 575.3 E(): 1.4e-24; 36.4% identity in 313 aa overlap. Contains possible hydrophobic membrane spanning regions 1 1 4 16 [Reporter]SCO6734 (6E24)_PCR/81 [Gene]SCO6734/75 NC_003888 SC5F2A.17, probable amino acid permease, len: 489aa; similar to many especially SW:ANSP_SALTY AnsP, L-asparagine permease from Salmonella enterica (497 aa) fasta scores; opt: 1886, z-score: 2042.9, E(): 0, (58.6% identity in 476 aa overlap). Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease and Prosite match to PS00218 Amino acid permeases signature. Also contains several probable membrane spanning hydrophobic regions. 3 4 3 21 [Reporter]SCO0726 (2G1)_PCR/7119 [Gene]SCO0726/6491 NC_003888 3SC5B7.04c, possible oxidoreductase, len: 162 aa; similar to TR:Q9K416 (EMBL:AL359779) Streptomyces coelicolor putative flavin-dependent reductase SCG22.29c, 169 aa; fasta scores: opt: 184 z-score: 248.1 E(): 2.7e-06; 34.1% identity in 132 aa overlap. Contains Pfam match to entry PF01613 Flavin_Reduct, Flavin reductase like domain 1 1 4 15 [Reporter]SCO1436 (7A24)_PCR/80 [Gene]SCO1436/74 NC_003888 SC6D7.03, possible secreted protein, len: 443 aa. Highly similar to many proteins of unknown function including: Mycobacterium tuberculosis SW:Y0D3_MYCTU(EMBL:Z78020) hypothetical 36.7 KD protein (345 aa), fasta scores opt: 1088 z-score: 1211.2 E():0 44.1% identity in 447 aa overlap and Streptomyces coelicolor TR:CAB53286(EMBL:AL109972) putative integral membrane protein SCJ9A.25C (445 aa), fast scores opt: 1023 z-score: 1139.2 E():0 42.4% identity in 429 aa overlap. Also similar to the adjoining CDS SC6D7.04 (347 aa), fasta scores opt: 651 z-score: 585.4 E(): 3e-27 35.9% identity in 343 aa overlap. Contains a Pfam matches to entries PF01595 DUF21, Domain of unknown function and 2x Pfam match to entry PF00571 CBS, CBS domain. Also contains possible hydrophobic membrane spanning regions 3 4 3 20 [Reporter]SCO4605 (3C1)_PCR/7118 [Gene]SCO4605/6490 NC_003888 SCD39.05, nuoK2, NADH dehydrogenase subunit, len: 130 aa; similar to SW:NULC_SYNY3 (EMBL:X62517) Synechocystis sp. NADH-plastoquinone oxidoreductase chain 4L (EC 1.6.5.3) NdhE, 103 aa; fasta scores: opt: 315 z-score: 414.1 E(): 1.3e-15; 50.0% identity in 100 aa overlap and to TR:Q9XAR4 (EMBL:AL078618) Streptomyces coelicolor SCD16A.11c, NuoK, NADH dehydrogenase subunit, 99 aa; fasta scores: opt: 251 z-score: 292.3 E(): 1.2e-10; 48.9% identity in 90 aa overlap. Contains Pfam match to entry PF00420 oxidored_q2, NADH-ubiquinone/plastoquinone oxidoreductase chain 4L and possible hydrophobic membrane spanning regions 1 1 4 14 [Reporter]SCO7180 (8M20)_PCR/79 [Gene]SCO7180/73 NC_003888 SC8A11.08c, hypothetical protein, len: 117 aa; similar to TR:AAF70105 (EMBL:AF257324) Streptomyces galilaeus polyketide oxygenase AknX, 122 aa; fasta scores: opt: 151 z-score: 213.3 E(): 0.00021; 34.3% identity in 105 aa overlap 1 1 4 13 [Reporter]SCO6452 (9I20)_PCR/78 [Gene]SCO6452/72 NC_003888 SC9B5.19, probable transport permease protein, len: 334aa; second of a five gene operon probably involved in peptide uptake, similar to many transport permeases eg. SW:DPPB_ECOLI DppB, dipeptide transport system permease protein from Escherichia coli (339 aa) fasta scores: opt: 362, z-score: 742.8, E(): 0, (32.9% identity in 340 aa overlap). 1 1 4 12 [Reporter]SCO2305 (10E20)_PCR/77 [Gene]SCO2305/71 NC_003888 SCC30.13, probable ABC transporter ATP-binding subunit, len: 308 aa; similar to TR:AAF12291 (EMBL:AE001862) Deinococcus radiodurans ABC transporter, ATP-binding protein, putative, DRA0007, 301 aa; fasta scores: opt: 753 z-score: 842.3 E(): 0; 47.9% identity in 288 aa overlap and to SW:NODI_RHILO (EMBL:X55705) Rhizobium loti nodulation ATP-binding protein I, NodI, 339 aa; fasta scores: opt: 576 z-score: 645.7 E(): 1.6e-28; 37.3% identity in 303 aa overlap. Also similar to various Streptomyces coelicolor ABC transporter ATP-binding proteins, e.g. TR:CAB56355 (EMBL:AL118514) S. coelicolor ABC transport protein, ATP-binding subunit SCD25.11c, 326 aa; fasta scores: opt: 606 z-score: 418.1 E(): 8.6e-18; 41.6% identity in 296 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 1 4 11 [Reporter]SCO4592 (11A20)_PCR/76 [Gene]SCO4592/70 NC_003888 SCD20.10c, possible integral membrane protein, len: 362 aa; similar to SW:RARD_ECOLI (EMBL:L02122) Escherichia coli RarD protein, 296 aa; fasta scores: opt: 792 z-score: 820.5 E(): 0; 42.3% identity in 291 aa overlap. Contains Pfam match to entry PF00892 DUF6, Integral membrane protein DUF6 and possible hydrophobic membrane spanning regions 3 4 3 19 [Reporter]SCO1397 (3O21)_PCR/7117 [Gene]SCO1397/6489 NC_003888 SC1A8A.17, unknown, len: 339aa; 3 4 3 18 [Reporter]SCO6226 (4K21)_PCR/7116 [Gene]SCO6226/6488 NC_003888 SC2H4.08c, unknown, len: 508 aa; some similarity to Rhizobium sp. NGR234 plasmid pNGR234a hypothetical protein Y4XN_RHISN (628 aa), fasta scores; opt: 116 z-score: 207.6 E(): 0.00026, 26.4% identity in 641 aa overlap. Also some similarity to divergent CDS SC2H4.09 (E(): 0.00044, 29.1% identity in 553 aa overlap) 3 4 3 17 [Reporter]SCO7122 (5G21)_PCR/7115 [Gene]SCO7122/6487 NC_003888 SC4B10.23, possible acetyltransferase, len: 163 aa; similar to TR:Q9RCV6 (EMBL:AL136149) Streptomyces coelicolor putative acetyltransferase SCM2.08c, 168 aa; fasta scores: opt: 111 z-score: 148.3 E(): 0.86; 30.5% identity in 118 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 3 4 3 16 [Reporter]SCO7578 (6C21)_PCR/7114 [Gene]SCO7578/6486 NC_003888 SC5F1.32c, unknown, len: 205 aa 1 1 4 10 [Reporter]SCO2903 (12M16)_PCR/75 [Gene]SCO2903/69 NC_003888 SCE19A.03c, possible secreted protein, len: 134 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains probable N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site, so may be a lipoprotein 3 4 3 15 [Reporter]SCO2125 (7O17)_PCR/7113 [Gene]SCO2125/6485 NC_003888 SC6E10.19c, hypothetical protein, len: 362 aa; unknown function, weak similarity to Y63D3A.4 (EMBL:AL032652) Caenorhabditis elegans hypothetical protein (362 aa), fasta scores; opt: 172 z-score: 209.3 E(): 0.00025, 28.7% identity in 181 aa overlap 1 1 4 9 [Reporter]SCO1616 (13I16)_PCR/74 [Gene]SCO1616/68 NC_003888 SCI35.38c, probable transcriptional regulator, len: 325 aa; similar to many members of the lysR family CBBR_RHORU RUBISCO operon transcriptional regulator (298 aa), fasta scores; opt: 394 z-score: 410.2 E(): 1.3e-15, 30.2% identity in 295 aa overlap. Contains PS00044 Bacterial regulatory proteins, lysR family signature, possible helix-turn-helix motif at aa 53-74 (Score 1391, +3.92 SD), and Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix proteins, lysR family, score 129.30, E-value 7e-35 3 4 3 14 [Reporter]SCO2016 (8K17)_PCR/7112 [Gene]SCO2016/6484 NC_003888 SC7H2.30c, possible monooxygenase, len: 470 aa; similar to many e.g. SW:IUCD_ECOLI L-lysine 6-monooxygenase from Escherichia coli (425 aa) fasta scores; opt: 393, z-score: 466.0, E(): 1.2e-18, (34.4% identity in 425 aa overlap). 1 1 4 8 [Reporter]SCO3850 (14E16)_PCR/73 [Gene]SCO3850/67 NC_003888 SCH69.20c, possible integral membrane protein, len: 352 aa; contains possible membrane spanning hydrophobic region. Similar to neighbouring gene SCH69.19c fasta scores; opt: 651, z-score: 547.1, E(): 2.3e-25, (45.9% identity in 218 aa overlap) 3 4 3 13 [Reporter]SCO5745 (9G17)_PCR/7111 [Gene]SCO5745/6483 NC_003888 SC9A10.09, unknown, len: 561 aa; similar to many members of the UPF0036 family e.g. YOR4_CORGL (645 aa), fasta scores; opt: 1873 z-score: 1500.1 E(): 0, 55.6% identity in 493 aa overlap. Contains Pfam match to entry PF00753 lactamase_B, Metallo-beta-lactamase superfamily, score 132.40, E-value 8.3e-36 1 1 4 7 [Reporter]SCO3626 (15A16)_PCR/72 [Gene]SCO3626/66 NC_003888 SCH10.04c, probable RNA polymerase sigma factor, len: 294 aa; similar to many e.g. TR:O05735 (EMBL:U87308) SigE, Mycobacterium avium extracytoplasmic function alternative sigma factor (251 aa), fasta scores; opt: 226 z-score: 266.2 E(): 1.6e-07, 29.8% identity in 168 aa overlap 3 4 3 12 [Reporter]SCO2448 (10C17)_PCR/7110 [Gene]SCO2448/6482 NC_003888 SCC24.19, unknown, len: 554 aa 1 1 4 6 [Reporter]SCO1696 (16M12)_PCR/71 [Gene]SCO1696/65 NC_003888 SCI30A.17, unknown, len: 244 aa; similar to TR:P71976 (EMBL:Z81360) hypothetical protein from Mycobacterium tuberculosis (272 aa) fasta scores; opt: 192, z-score: 223.1, E(): 4.3e-05, (26.7% identity in 236 aa overlap) and to TR:Q98JP6 (EMBL:AP002998) Rhizobium loti (Mesorhizobium loti) MLL1845 241 aa; fasta scores: opt: 534 Z-score: 595.0 E(): 1.7e-25; 41.564% identity in 243 aa overlap 3 4 3 11 [Reporter]SCO4700 (11O13)_PCR/7109 [Gene]SCO4700/6481 NC_003888 SCD31.25, unknown, len: 149 aa 1 1 4 5 [Reporter]SCO3562 (17I12)_PCR/70 [Gene]SCO3562/64 NC_003888 SCH5.25, possible integral membrane transport, len: 830aa; contains possible membrane spanning hydrophobic regions. C-terminal region (600-800aa) similar to carbonic anhydrases eg. SW:CYNT_ECOLI carbonic anhydrase from Escherichia coli fasta scores; opt: 342, z-score: 333.5, E(): 2.9e-11, (35.7% identity in 210 aa overlap). Also N-terminal half has weak similarity to several proposed sulphate transporters e.g TR:Q55814 (EMBL:D64004) low affinity sulphate transporter from Synechocystis sp. PCC6803 fasta scores; opt: 419, z-score: 400.7, E(): 5.2e-15, (30.0% identity in 420 aa overlap). Contains Pfam matches to entry PF00484 Pro_CA, Prokaryotic-type carbonic anhydrases, score 131.20, E-value 5.4e-37 and entry PF00916 Sulfate_transp, Sulfate transporter family, score 119.90, E-value 4.9e-32. 3 4 3 10 [Reporter]SCO3514 (12K13)_PCR/7108 [Gene]SCO3514/6480 NC_003888 SCE134.15, possible integral membrane ATP-binding protein, len: 469aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). Also contains possible membrane spanning hydrophobic regions. 1 1 4 4 [Reporter]SCO0292 (18E12)_PCR/69 [Gene]SCO0292/63 NC_003888 SC5G9.01, probable sugar ABC-transporter integral membrane protein, partial CDS, len: >148 aa; similar to e.g. TR:O51925 (EMBL:AF012836), malG, Thermococcus litoralis inner membrane protein from trehalose/maltose ABC-transporter system (278 aa), fasta scores; opt: 296 z-score: 384.7 E(): 4.6e-14, 32.2% identity in 146 aa overlap. Also similar to many putative ABC-transporter components e.g. SW:YOR2_CALSR (EMBL:L18965) Caldicellulosiruptor sp. (strain Rt8B.4) putative membrane bound sugar transport protein ORF2 (275 aa) (43.0% identity in 142 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component,SCF85.20, possible transmembrane transport protein, partial CDS, len: >185 aa. Similar to Streptomyces lividans TR:Q9ZI16 (EMBL; AF043654) BxlG (272 aa), fasta scores opt: 377 z-score: 450.9 E(): 9.2e-18 39.9% identity in 158 aa overlap and Streptomyces coelicolor TR:O50501 (EMBL; AL009199) probable transmembrane transport protein SCSt7b7.04 (302 aa), fasta scores opt: 288 z-score: 346.2 E(): 6.2e-12 29.3% identity in 181 aa overlap. Contains multiple potential membrane spanning hydrophobic domains. 1 1 4 3 [Reporter]SCP1.146 (19A12)_PCR/68 [Gene]SCP1.146/62 NC_003888 SCP1.146, possible ATP/GTP-binding protein, len: 848aa; weak but full length similarity with TR:Q9L4H0 (EMBL:AJ243106) conjugative protein from integrative element ICESt1 of Streptococcus thermophilus (834 aa) fasta scores; opt: 190, z-score: 212.3, E(): 0.00023, 18.6% identity in 870 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 1 4 2 [Reporter]SCO4055 (20I8)_PCR/67 [Gene]SCO4055/61 NC_003888 2SCD60.21c, probable alcohol dehydrogenase, len: 377 aa; similar to SW:FADH_PSEPU (EMBL:D21201) Pseudomonas putida glutathione-independent formaldehyde dehydrogenase (EC 1.2.1.46) FdhA, 398 aa; fasta scores: opt: 1048 z-score: 1080.0 E(): 0; 47.9% identity in 390 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and match to Prosite entry PS00059 Zinc-containing alcohol dehydrogenases signature 1 1 3 22 [Reporter]SCO4195 (1I4)_PCR/65 [Gene]SCO4195/60 NC_003888 2SCD46.09, possible ATP/GTP binding protein, len: 684aa; similar to TR:CAB93076 (EMBL:AL357432) putative ATP/GTP binding protein from Streptomyces coelicolor (681 aa) fasta scores; opt: 1940, z-score: 2059.2, E(): 0, 49.6% identity in 682 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 4 3 9 [Reporter]SCO3315 (13G13)_PCR/7107 [Gene]SCO3315/6479 NC_003888 SCE68.13, probable transcriptional regulator, len: 230 aa; similar to TR:O53612 (EMBL:AL021428) Mycobacterium tuberculosis putative transcriptional regulator (189 aa), fasta scores; opt: 315 z-score: 346.2 E(): 5.8e-12, 35.5% identity in 172 aa overlap. Weak similarity to TR:O54180 (EMBL:AL021411), SC7H1.22, S.coelicolor probable transcriptional regulator (203 aa) (34.4% identity in 154 aa overlap). Contains probable helix-turn-helix motif at aa 50-71 (Score 1594, +4.62 SD). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family (weak match) 3 4 3 8 [Reporter]SCO3160 (14C13)_PCR/7106 [Gene]SCO3160/6478 NC_003888 SCE87.11, possible integral membrane transport protein, len: 253 aa. Similar to many proteins thought to be involved in the transport of cobalt e.g. Streptomyces coelicolor TR:O54188(EMBL:AL021411) cobalt transport integral membrane protein SC7H1.29c (CbiQ) (249 aa), fasta scores opt: 196 z-score: 249.6 E(): 1.6e-06 25.5% identity in 251 aa overlap and Methanobacterium thermoautotrophicum TR:O27740(EMBL:AE000928) cobalt transport membrane protein (261 aa), fasta scores opt: 332 z-score: 417.0 E(): 7.5e-16 30.2% identity in 212 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 3 4 3 7 [Reporter]SCO0615 (15O9)_PCR/7105 [Gene]SCO0615/6477 NC_003888 SCF55.39, probable secreted protein, len: 152 aa. Contains N-terminal region signal peptide sequence 3 4 3 6 [Reporter]SCO2965 (16K9)_PCR/7104 [Gene]SCO2965/6476 NC_003888 SCE59.24, possible transport protein, len: 366 aa; similar to TR:CAB59494 (EMBL:AL132648) Streptomyces coelicolor putative transport protein SCI41.24c, 405 aa; fasta scores: opt: 513 z-score: 569.7 E(): 2.6e-24; 31.1% identity in 366 aa overlap. Contains possible hydrophobic membrane spanning regions 1 1 3 21 [Reporter]SCO0774 (2E4)_PCR/64 [Gene]SCO0774/59 NC_003888 3SCF60.06c, probable cytochrome P450, len: 412 aa; almost identical to TR:O85697 (EMBL:AF072709) Streptomyces lividans putative cytochrome P450 oxidoreductase, 406 aa and highly similar to SW:CPXH_STRGR (EMBL:X63601) Streptomyces griseus cytochrome P450 SoyC, 412 aa; fasta scores: opt: 1894 z-score: 2107.2 E(): 0; 67.5% identity in 412 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450 and match to Prosite entry PS00086 Cytochrome P450 cysteine heme-iron ligand signature 3 4 3 5 [Reporter]SCO3726 (17G9)_PCR/7103 [Gene]SCO3726/6475 NC_003888 SCH22A.04c, unknown, len: 206 aa 1 1 3 20 [Reporter]SCO5441 (3A4)_PCR/63 [Gene]SCO5441/58 NC_003888 SC6A11.17c, pep2A, hypothetical protein, len: 464 aa; previously sequenced as TR:O54204 (EMBL:AJ001205) 3 4 3 4 [Reporter]SCO3399 (18C9)_PCR/7102 [Gene]SCO3399/6474 NC_003888 SCE9.06, hypothetical protein, len: 167 aa; probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 1 1 3 19 [Reporter]SCO6150 (3M24)_PCR/62 [Gene]SCO6150/57 NC_003888 SC1A9.14, possible ADA-like regulatory protein, len: 477 aa; similar to ADA_MYCTU (EMBL:Z73902) putative ADA regulatory protein from Mycobacterium tuberculosis (496 aa) fasta scores; opt: 1135, z-score: 1376.3, E(): 0, (51.6% identity in 486 aa overlap). Also note N-terminal region similar to N-terminal region of ADA_ECOLI (EMBL:M10211) ADA regulatory protein from Escherichia coli (354 aa) fasta scores; opt: 302, z-score: 255.8, E(): 5.7e-07, (34.5% identity in 171 aa overlap) and C-terminal region similar to 3MG2_ECOLI AlkA, 3-methyladenine DNA glycosylase II involved in DNA repair (282 aa) fasta scores; opt: 171, z-score: 232.9, E(): 1.1e-05, (30.0% identity in 290 aa overlap). Contains Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family, score 72.90, E-value 6.7e-18 and PS00041 Bacterial regulatory proteins, araC family signature. 3 4 3 2 [Reporter]SCO4029 (20G5)_PCR/7100 [Gene]SCO4029/6473 NC_003888 2SC10A7.33, hypothetical protein, len: 249 aa; similar to TR:CAB76342 (EMBL:AL158061) Streptomyces coelicolor hypothetical 26.1 kDa protein SC6D11.19c, 236 aa; fasta scores: opt: 330 z-score: 392.0 E(): 2.5e-14; 32.7% identity in 226 aa overlap 1 1 3 18 [Reporter]SCO7082 (4I24)_PCR/61 [Gene]SCO7082/56 NC_003888 SC3A4.08c, doubtful CDS, len: 154 aa. 3 4 2 22 [Reporter]SCO5183 (1G1)_PCR/7098 [Gene]SCO5183/6472 NC_003888 2SC3B6.07, possible ATP-dependent DNA helicase, len: 1159 aa; similar to TR:O53347 (EMBL:AL021646) Mycobacterium tuberculosis putative ATP-dependent DNA helicase, MTV014.45c, 1101 aa; fasta scores: opt: 349 z-score: 368.0 E(): 5e-13; 29.2% identity in 1144 aa overlap to SW:UVRD_ECOLI (EMBL:M87049) Escherichia coli DNA helicase II (EC 3.6.1.-) UvrD, 720 aa; fasta scores: opt: 241 z-score: 255.5 E(): 9.2e-07; 26.3% identity in 800 aa overlap and to Streptomyces coelicolor 2SC3B6.08, 1222 aa; fasta scores: opt: 406 z-score: 336.0 E(): 4.2e-13;30.4% identity in 977 aa overlap. Contains Pfam match to entry PF00580 UvrD-helicase, UvrD/REP helicase and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Also contains 5x conserved repeat unit: ADAD 1 1 3 17 [Reporter]SCO6560 (5E24)_PCR/60 [Gene]SCO6560/55 NC_003888 SC4B5.10c, respiratory chain oxidoreductase, len: 646 aa; C-terminal region is similar to the N-terminal region of many proteins involved in electron transfer e.g. TR:O27592 (EMBL:AE000915) NADP-reducing hydrogenase, subunit C from Methanobacterium thermoautotrophicum (630 aa), fasta scores; opt: 121,4 z-score: 1170.7, E(): 0, (47.1% identity in 397 aa overlap). Contains PS00645 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. 2 3 9 3 [Reporter]SCP1.107 (19F14)_PCR/4714 [Gene]SCP1.107/4299 NC_003888 SCP1.107, unknown, len: 127aa; 3 4 2 21 [Reporter]SCO4913 (2C1)_PCR/7097 [Gene]SCO4913/6471 NC_003888 SCK13.05c, probable aldehyde dehydrogenase, len: aa; similar to SW:DHA5_YEAST (EMBL:X85987) Saccharomyces cerevisiae aldehyde dehydrogenase 2 (EC 1.2.1.5) Ald5, 506 aa; fasta scores: opt: 1336 z-score: 1519.8 E(): 0; 46.1% identity in 462 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family and match to Prosite entry PS00070 Aldehyde dehydrogenases cysteine active site 1 1 3 16 [Reporter]SCO6730 (6A24)_PCR/59 [Gene]SCO6730/54 NC_003888 SC5F2A.13, possible racemase, len: 398aa; similar to many egs. TR:O06543 (EMBL:Z95584) hypothetical protein from Mycobacterium tuberculosis (360 aa) fasta scores; opt: 1338, z-score: 1408.4, E(): 0, (57.1% identity in 361 aa overlap) and TR:O09176 (EMBL:U89906) alpha-methylacyl-CoA racemase from Mus musculus (moues) (361 aa) fasta scores; opt: 953, z-score: 1005.2, E(): 0, (44.2% identity in 344 aa overlap). Also similar to SC6A5.34 (EMBL:AL049485) probable fatty acid co-A racemase from Streptomyces coelicolor (387 aa) fasta scores; opt: 1787, z-score: 1558.9, E(): 0, (69.9% identity in 382 aa overlap). 2 3 9 2 [Reporter]SCO3434 (20N10)_PCR/4713 [Gene]SCO3434/4298 NC_003888 SCE36.01, partial CDS, possible DNA polymerase I, len: >328 aa; similar to the C-terminal region many e.g. SW:DPO1_CHLAU DNA polymerase I from Chloroflexus aurantiacus (942 aa) fasta scores; opt: 287, z-score: 322.2, E(): 1.3e-10, (28.9% identity in 305 aa overlap). Contains two Pfam matches to entry PF00476 DNA_pol_A, DNA polymerase family A.,SCE9.41, hypothetical protein, partial CDS, len: >268 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 3 4 2 20 [Reporter]SCO4948 (2O21)_PCR/7096 [Gene]SCO4948/6470 NC_003888 2SCK31.08, narH3, nitrate reductase beta chain, len: 548 aa; highly similar to SW:NARH_BACSU (EMBL:Z49884) Bacillus subtilis nitrate reductase beta chain NarH, 487 aa; fasta scores: opt: 2088 z-score: 2047.9 E(): 0; 58.2% identity in 483 aa overlap and to TR:O86716 (EMBL:AL031515) Streptomyces coelicolor NarH, putative nitrate reductase beta chain SC5C7.19, 531 aa; fasta scores: opt: 2542 z-score: 2343.2 E(): 0; 71.6% identity in 503 aa overlap 1 1 3 15 [Reporter]SCO4422 (7M20)_PCR/58 [Gene]SCO4422/53 NC_003888 SC6F11.20, possible hydrolase, len: 252 aa; similar to TR:Q9X7R7 (EMBL:AL049863) Streptomyces coelicolor putative hydrolase SC5H1.09c, 265 aa; fasta scores: opt: 465 z-score: 555.8 E(): 1.9e-23; 40.1% identity in 252 aa overlap 2 3 8 22 [Reporter]SCO4218 (1N6)_PCR/4711 [Gene]SCO4218/4297 NC_003888 2SCD46.32, possible small hydrophilic protein, len: 63 aa 1 1 3 14 [Reporter]SCO7176 (8I20)_PCR/57 [Gene]SCO7176/52 NC_003888 SC8A11.04c, probable secreted peptidase, len: 1253 aa; similar to TR:Q9RL54 (EMBL:AL121596) Streptomyces coelicolor probable secreted peptidase SCF51A.10, 1245 aa; fasta scores: opt: 1827 z-score: 1848.3 E(): 0; 37.2% identity in 1300 aa overlap, to TR:P95684 (EMBL:D83672) Streptomyces albogriseolus subtilisin-like protease, 1102 aa; fasta scores: opt: 1365 z-score: 1380.6 E(): 0; 33.4% identity in 1268 aa overlap and to Streptomyces coelicolor probable secreted peptidase SC8A11.16c, 1239 aa; fasta scores: opt: 1835 z-score: 1467.4 E(): 0; 46.9% identity in 1279 aa overlap. Contains Pfam matches to entries PF00082 Peptidase_S8, Subtilase family and PF02225 PA, PA domain and matches to Prosite entries PS00136 Serine proteases, subtilase family, aspartic acid active site, PS00137 Serine proteases, subtilase family, histidine active site and PS00138 Serine proteases, subtilase family, serine active site. Also contains possible N-terminal region signal peptide sequence 2 3 8 21 [Reporter]SCO2617 (2J6)_PCR/4710 [Gene]SCO2617/4296 NC_003888 SCC80.02c, clpX, ATP dependent Clp Protease ATP binding subunit, len: 428 aa; highly similar to SW:CLPX_BACSU (EMBL:X95306) Bacillus subtilis ATP-dependent Clp protease ATP-binding subunit ClpX, 420 aa; fasta scores: opt: 1786 z-score: 1870.3 E(): 0; 65.8% identity in 418 aa overlap. Contains Pfam match to entry PF00004 AAA, ATPases associated with various cellular activities (AAA) and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 1 3 13 [Reporter]SCO7163 (9E20)_PCR/56 [Gene]SCO7163/51 NC_003888 SC9A4.25c, possible transferase, len: 270 aa; similar to TR:CAB87566 (EMBL:AJ277295) Sphingomonas sp. putative acyl transferase FldZ protein, 224 aa; fasta scores: opt: 286 z-score: 338.7 E(): 2.1e-11; 27.4% identity in 223 aa overlap 2 3 8 19 [Reporter]SCO6928 (4B6)_PCR/4708 [Gene]SCO6928/4295 NC_003888 SC1B2.34c, possible O-methyltransferase, len: 696 aa. Contains a central region which is similar to many protein-L-isoaspartate O-methyltransferases e.g. Escherichia coli SW:PIMT_ECOLI (EMBL:M63493) (207 aa), fasta scores opt: 301 z-score: 340.3 E(): 1.7e-11 31.5% identity in 213 aa overlap and Streptomyces coelicolor TR:CAB71208 (EMBL:AL138538) SC6D10.13C (324 aa), fasta scores opt: 442 z-score: 493.8 E(): 4.8e-20 36.9% identity in 306 aa overlap. Contains a Pfam match to entry PF01135 PCMT, Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT). 1 1 3 12 [Reporter]SCO2301 (10A20)_PCR/55 [Gene]SCO2301/50 NC_003888 SCC30.09c, hypothetical protein, len: 285 aa; similar to TR:O69481 (EMBL:AL023635) Mycobacterium leprae hypothetical 40.3 kD protein MLCB1243.36, 385 aa; fasta scores: opt: 414 z-score: 484.0 E(): 1.7e-19; 34.9% identity in 370 aa overlap. Contains Pfam match to entry PF01784 DUF34, Domain of unknown function 2 3 8 18 [Reporter]SCO6848 (5N2)_PCR/4707 [Gene]SCO6848/4294 NC_003888 SC3D9.16c, unknown, len: 152 aa; similar to TR:Q9PCL6 (EMBL:AE003998) Xylella fastidiosa hypothetical protein XF1762, 172 aa; fasta scores: opt: 501 Z-score: 598.8 E(): 1e-25; 55.484% identity in 155 aa overlap 2 3 8 17 [Reporter]SCO6652 (6J2)_PCR/4706 [Gene]SCO6652/4293 NC_003888 SC5A7.02, unknown, len: 461 aa; contains 6 degenerate repeats of P(AT)ESAVPA 2 3 8 16 [Reporter]SCO0307 (7F2)_PCR/4705 [Gene]SCO0307/4292 NC_003888 SC5G9.16, possible DNA-binding protein, len: 295 aa; probable CDS suggested by GC frameplot and positional base preference. Similar to possible DNA-binding proteins from S. coelicolor e.g. SCJ9A.15c (cosmid SCJ9A), S. coelicolor possible DNA-binding protein (290 aa) (39.3% identity in 298 aa overlap). Contains probable helix-turn-helix motif at residues 35-56 (Score 1197, +3.26 SD) 2 3 8 15 [Reporter]SCO2149 (8B2)_PCR/4704 [Gene]SCO2149/4291 NC_003888 SC6G10.22c, qcrA, Rieske iron-sulfur protein, len: 353 aa; identical over the partial sequence of QcrA, Rieske iron-sulfur protein from Streptomyces lividans (170 aa) fasta scores; opt: 1174, z-score: 1332.,1 E(): 0, (100.0% identity in 169 aa overlap). SW:UCRI_BRAJA ubiquinol cytochrome C reductase iron-sulphur subunit (176 aa) fasta scores; opt: 156, z-score: 186.0, E(): 0.0047, (33.3% identity in 84 aa overlap). Contains Prosite match to PS00200 Rieske iron-sulfur protein signature 2 and possible hydrophobic membrane spanning regions 1 1 20 22 [Reporter]SCO1358 (1M20)_PCR/439 [Gene]SCO1358/399 NC_003888 2SCG61.40c, possible LysR-family transcriptional regulator, len: 303 aa; similar to many e.g. SW:P11721 (OXYR_ECOLI) hydrogen peroxide-inducible genes activator from Escherichia coli (305 aa) fasta scores; opt: 359, z-score: 409.3, E(): 2.5e-15, 27.6% identity in 297 aa overlap and TR:Q9RDH0 (EMBL:AL136519) putative LysR-family transcriptional regulator from Streptomyces coelicolor (313 aa) fasta scores; opt: 897, z-score: 1010.0, E(): 0, 49.0% identity in 294 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and Prosite match to PS00044 Bacterial regulatory proteins, lysR family signature. 2 3 8 14 [Reporter]SCO7197 (8N22)_PCR/4703 [Gene]SCO7197/4290 NC_003888 SC8A11.25c, probable amino acid transport integral membrane protein, len: 512 aa; similar to SW:ALST_BACSU (EMBL:Z73234) Bacillus subtilis amino acid carrier protein AlsT, 465 aa; fasta scores: opt: 560 z-score: 628.4 E(): 1.6e-27; 35.2% identity in 454 aa overlap. Contains Pfam match to entry PF01235 Na_Ala_symp, Sodium:alanine symporter family and possible hydrophobic membrane spanning regions 1 1 20 21 [Reporter]SCO2726 (2I20)_PCR/438 [Gene]SCO2726/398 NC_003888 SCC46.11c, msdA, methylmalonic acid semialdehyde dehydrogenase, 500 aa; identical to previously sequenced TR:Q53935 (EMBL:L48550) Streptomyces coelicolor methylmalonic acid semialdehyde dehydrogenase MsdA and highly similar to SW:MMSA_PSEAE (EMBL:M84911) Pseudomonas aeruginosa methylmalonate-semialdehyde dehydrogenase [acylating] (EC 1.2.1.27) MmsA, 496 aa; fasta scores: opt: 1652 z-score: 1799.5 E(): 0; 48.0% identity in 490 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family and match to Prosite entry PS00070 Aldehyde dehydrogenases cysteine active site 1 1 20 20 [Reporter]SCO7375 (3E20)_PCR/437 [Gene]SCO7375/397 NC_003888 SC10G8.02, possible acetyltransferase, len: 264 aa. Similar to Deinococcus radiodurans TR:AAF12253(EMBL:AE001862) putative acetyltransferase (210 aa), fasta scores opt: 272 z-score: 314.3 E(): 4.3e-10 30.9% identity in 207 aa overlap. Contains a Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. Contains a 7xDT repeat region in the C-terminus. 1 1 20 19 [Reporter]SCO5619 (4A20)_PCR/436 [Gene]SCO5619/396 NC_003888 SC2E1.36, unknown prophage gene, len: 168 aa 1 1 20 18 [Reporter]SCO7692 (5M16)_PCR/435 [Gene]SCO7692/395 NC_003888 SC4C2.27, unknown, len: 89 aa 1 1 20 17 [Reporter]SCO2183 (6I16)_PCR/434 [Gene]SCO2183/394 NC_003888 SC5F7.18, aceE1, probable pyruvate dehydrogenase E1 component, len: 899 aa; similar to many eg. SW:ODP1_ALCEU pyruvate dehydrogenase E1 component from Alcaligenes eutrophus fasta scores; opt: 2508, z-score: 2834.4, E(): 0, (51.3% identity in 887 aa overlap). 1 1 20 16 [Reporter]SCO6179 (7E16)_PCR/433 [Gene]SCO6179/393 NC_003888 SC6C5.15, probable nucleotide-sugar dehydratase, len: 330 aa; similar to many e.g. TR:Q54144 (EMBL:U08223) dTDP-glucose dehydratase from Streptomyces fradiae (333 aa) fasta scores; opt: 581, z-score: 563.5, E(): 4.3e-24, (38.4% identity in 318 aa overlap). 1 1 20 15 [Reporter]SCO5235 (8A16)_PCR/432 [Gene]SCO5235/392 NC_003888 SC7E4.32, possible sugar hydrolase, len: 496 aa; similar to SW:HEXA_ALTSO (EMBL:D17399) Alteromonas sp. beta-hexosaminidase A precursor (EC 3.2.1.52) Cht60, 598 aa; fasta scores: opt: 486 z-score: 525.0 E(): 9e-22; 34.4% identity in 363 aa overlap and to TR O69862 (EMBL:AL023702;) Streptomyces coelicolor putative hydrolase SC1C3.20c, 506 aa; fasta scores: opt: 1849 z-score: 1988.9 E(): 0; 60.6% identity in 498 aa overlap. Contains Pfam match to entry PF00933 Glyco_hydro_3, Glycosyl hydrolase family 3 N terminal domain 1 1 20 14 [Reporter]SCO5406 (9M12)_PCR/431 [Gene]SCO5406/391 NC_003888 SC8F4.10c, hypothetical protein, len: 258 aa; similar to TR:AAF10203 (EMBL:AE001920) hypothetical protein from Deinococcus radiodurans (254 aa) fasta scores; opt: 157, z-score: 174.0, E(): 0.028, 27.8% identity in 245 aa overlap. Also weakly similar to the neighbouring hypothetical CDSs SC8F4.11c and SC8F4.12c. 1 1 20 13 [Reporter]SCO2584 (10I12)_PCR/430 [Gene]SCO2584/390 NC_003888 SCC123.22c, possible membrane protein, len: 228 aa. Contains a possible membrane spanning hydrophobic domain. 3 4 2 19 [Reporter]SCO1392 (3K21)_PCR/7095 [Gene]SCO1392/6469 NC_003888 SC1A8A.12, unknown, len: 300aa; 3 4 2 18 [Reporter]SCO6181 (4G21)_PCR/7094 [Gene]SCO6181/6468 NC_003888 SC2G5.02, possible transferase, len: 344aa; similar to many eg. SW:EXOO_RHIME succinoglycan biosynthesis protein, ExoO, from Rhizobium meliloti (334 aa) fasta scores; opt:177, z-score: 202.6, E(): 0.00053, (29.5% identity in 278aa overlap). Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases, score 32.70,E-value 8.3e-06. 3 4 2 17 [Reporter]SCO7117 (5C21)_PCR/7093 [Gene]SCO7117/6467 NC_003888 SC4B10.18c, probable ferredoxin reductase, len: 420 aa; similar to TR:BAA94714 (EMBL:AB031319) Nocardioides sp. ferredoxin reductase PhdD, 414 aa; fasta scores: opt: 928 z-score: 1051.4 E(): 0; 40.0% identity in 420 aa overlap, to SW:THCD_RHOER (EMBL:U17130) Rhodococcus erythropolis rhodocoxin reductase (EC 1.18.1.-) ThcD, 426aa; fasta scores: opt: 853 z-score: 966.6 E(): 0; 39.5% identity in 370 aa overlap and to TR:CAB69757 (EMBL:AL137187) Streptomyces coelicolor putative reductase SC7A8.08c, 421 aa; fasta scores: opt: 830 z-score: 732.2 E(): 0; 50.8% identity in 415 aa overlap. Contains Pfam match to entry PF00070 pyr_redox, Pyridine nucleotide-disulphide oxidoreductase 3 4 2 16 [Reporter]SCO2169 (6O17)_PCR/7092 [Gene]SCO2169/6466 NC_003888 SC5F7.32, possible integral membrane protein, len: 251aa; contains possible membrane spanning hydrophobic regions. some similarity to TR:O32952 (EMBL:Z98741) hypothetical protein from Mycobacterium leprae (247 aa) fasta scores; opt: 335, z-score: 361.2, E(): 8.8e-13, (34.4% identity in 221 aa overlap). 1 1 3 11 [Reporter]SCO4517 (11M16)_PCR/54 [Gene]SCO4517/49 NC_003888 SCD35.24c, hypothetical protein, len: 241 aa; C-terminal region similar to TR:Q9KYA0 (EMBL:AL356813) Streptomyces coelicolor conserved hypothetical protein SCK15.04c, 216 aa; fasta scores: opt: 613 Z-score: 714.3 E(): 3.8e-32; 52.792% identity in 197 aa overlap 3 4 2 15 [Reporter]SCO2121 (7K17)_PCR/7091 [Gene]SCO2121/6465 NC_003888 SC6E10.15c, possible two-component system sensor kinase, len: 413 aa; similar to many e.g. SW:DEGS_BACBR (EMBL:L15444), DegS, Bacillus brevis sensor protein (386 aa), fasta scores; opt: 174 z-score: 193.4 E(): 0.0019, 23.4% identity in 278 aa overlap. The N-terminal approx. 100 aa are different from many of the putative homologues. Similar to others from S.coelicolor e.g. SCE126.08C (EMBL:AL049630) S.coelicolor possible two component sensor kinase (401 aa) (26.6% identity in 323 aa overlap). Contains hydrophobic, possible membrane-spanning regions in the N-terminal half 1 1 3 10 [Reporter]SCO3301 (12I16)_PCR/53 [Gene]SCO3301/48 NC_003888 SCE15.18, possible secreted protein, len: 283aa; contains possible N-terminal signal sequence. 3 4 2 14 [Reporter]SCO6858 (8G17)_PCR/7090 [Gene]SCO6858/6464 NC_003888 SC7F9.10c, unknown, len: 215 aa. Weakly similar to Streptomyces coelicolor TR:Q9X899 (EMBL:AL049645) hypothetical 25.5 KD protein, SCE2.04 (241 aa), fasta scores opt: 160 z-score: 176.7 E(): 0.02 28.3% identity in 205 aa overlap. 1 1 3 9 [Reporter]SCO1918 (13E16)_PCR/52 [Gene]SCO1918/47 NC_003888 SCI7.36c, possible integral membrane protein, len: 106aa; similar to many proposed to be involved in resistance mechanisms eg. SW:EMRE_ECOLI viologen resistance protein from Escherichia coli (110 aa) fasta scores; opt: 313, z-score: 392.7, E(): 1.5e-14, (51.0% identity in 100 aa overlap). Contains Pfam match to entry PF00893 DUF7, Integral membrane protein. 3 4 2 13 [Reporter]SCO5740 (9C17)_PCR/7089 [Gene]SCO5740/6463 NC_003888 SC9A10.04, possible membrane protein, len: 151 aa; similar to hypothetical proteins from Mycobacterium bovis and tuberculosis that follow dapB, e.g. YDAB_MYCBO hypothetical 19.3 kd protein in dapb 3'region (177 aa), fasta scores; opt: 377 z-score: 696.1 E(): 1.7e-31, 47.1% identity in 136 aa overlap 1 1 3 8 [Reporter]SCO3776 (14A16)_PCR/51 [Gene]SCO3776/46 NC_003888 SCH63.23, possible membrane protein, len: 180 aa. Contains possible hydrophobic membrane spanning region and 3x degenerate repeat: DTG(T/A). Also contains a TTA leucine codon, possible target for bldA regulation 3 4 2 12 [Reporter]SCO2323 (10O13)_PCR/7088 [Gene]SCO2323/6462 NC_003888 SCC53.14, possible integral membrane protein, len: 379aa; contains possible membrane-spanning hydrophobic regions. 1 1 3 6 [Reporter]SCO1914 (16I12)_PCR/49 [Gene]SCO1914/45 NC_003888 SCI7.32, unknown, len: 56aa; 2 3 8 13 [Reporter]SCO1490 (9J22)_PCR/4702 [Gene]SCO1490/4289 NC_003888 SC9C5.14c, probable NusB-family protein, len: 142 aa; similar to SW:NUSB_ECOLI (EMBL:X00681) Escherichia coli N utilization substance protein B NusB, 139 aa; fasta scores: opt: 183 z-score: 251.7 E(): 1.5e-06; 30.0% identity in 130 aa overlap. Contains Pfam match to entry PF01029 NusB, NusB family 3 4 2 11 [Reporter]SCO4695 (11K13)_PCR/7087 [Gene]SCO4695/6461 NC_003888 SCD31.20, hypothetical protein, len: 181 aa; similar to C-terminal part of many hypothetical proteins, e.g. TR:O54162 (EMBL:AL021411) Streptomyces coelicolor hypothetical 40.9 kD protein SC7H1.03c, 391 aa; fasta scores: opt: 594 z-score: 691.0 E(): 4.7e-31; 53.5% identity in 185 aa overlap 1 1 3 5 [Reporter]SCO1170 (17E12)_PCR/48 [Gene]SCO1170/44 NC_003888 2SCG11.04c, xylB, xylulose kinase (fragment), len: >81 aa; identical to N-terminal region of SW:XYLB_STRRU (EMBL:M73789) Streptomyces rubiginosus xylulose kinase (EC 2.7.1.17) XylB, 481 aa,SCG11A.01, xylB, xylulose kinase, len: >432 aa; highly similar to SW:XYLB_STRRU (EMBL:M73789) Streptomyces rubiginosus xylulose kinase (EC 2.7.1.17) (xylulokinase) XylB, 481 aa; fasta scores: opt: 2553 z-score: 2795.9 E(): 0; 89.2% identity in 427 aa overlap. Contains match to Pfam entry PF00370 FGGY, FGGY family of carbohydrate kinases and two Prosite matches to entries PS00933 FGGY family of carbohydrate kinases signature and PS00445 FGGY family of carbohydrate kinases signature 2 2 3 8 12 [Reporter]SCO2716 (10F22)_PCR/4701 [Gene]SCO2716/4288 NC_003888 SCC46.01c, possible secreted protein, len: > 171 aa; similar to TR:Q9X7U2 (EMBL:AL049863) Streptomyces coelicolor hypothetical 22.7 kD protein SC5H1.35c, 237 aa; fasta scores: opt: 456 z-score: 458.0 E(): 4.3e-18; 42.6% identity in 176 aa overlap. Contains possible N-terminal region signal peptide sequence,SCC61A.37c, possible secreted protein (fragment), len: >114aa; forms to C-terminus of TR:CAB75305 (EMBL:AL139164) putative secreted protein on an overlapping cosmid. Contains possible hydrophobic membrane spanning region 3 4 2 9 [Reporter]SCO1444 (13C13)_PCR/7085 [Gene]SCO1444/6460 NC_003888 SCL6.01, probable chitinase precursor, len: 791 aa; similar to TR:CAB62382 (EMBL:AJ250585) Arthrobacter sp chitinase precursor (EC 3.2.1.14) ChitIA, 880 aa; fasta scores: opt: 2788 z-score: 2817.6 E(): 0; 56.3% identity in 764 aa overlap and to SW:CHID_VIBFU (EMBL:U41418) Vibrio furnissii chitodextrinase precursor (EC 3.2.1.14) EndoI, 1046 aa; fasta scores: opt: 2055 z-score: 2075.7 E(): 0; 53.8% identity in 746 aa overlap. Contains three Pfam matches to entry PF00704 Glyco_hydro_18, Glycosyl hydrolases family 18 and match to Prosite entry PS01095 Chitinases family 18 active site. Contains also possible N-terminal region signal peptide sequence 1 1 3 4 [Reporter]SCO3382 (18A12)_PCR/47 [Gene]SCO3382/43 NC_003888 SCE94.33c, nadB, L-aspartate oxidase, len: 580 aa; similar to many eg. SW:NADB_ECOLI NadB, L-aspartate oxidase from Escherichia coli (540 aa) fasta scores; opt: 1207, z-score: 1289.2, E(): 0, (42.9% identity in 531 aa overlap). Contains Pfam match to entry PF00890 FAD_binding_2, FAD binding domain and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 2 3 8 11 [Reporter]SCO4083 (11B22)_PCR/4700 [Gene]SCO4083/4287 NC_003888 SCD25.19, unknown, len: 437aa. 1 1 3 3 [Reporter]SCO1883 (19M8)_PCR/46 [Gene]SCO1883/42 NC_003888 SCI7.01c, partial CDS, possible xylanase, len: >95aa; similar to the N-terminal region of many eg. TR:O30426 (EMBL:AF005383) xylanase from the thermophile Caldocellum saccharolyticum (1347 aa) fasta scores; opt: 263, z-score: 357.5, E(): 1.4e-12, (35.2% identity in 91 aa overlap).,SCI39.30c, probable xylanase (fragment), len: >458 aa; similar to TR:Q9WXE8 (EMBL:AB022865) Prevotella ruminicola xylosidase, 452 aa; fasta scores: opt: 553 Z-score: 572.6 bits: 115.2 E(): 2.6e-24; 37.954% identity in 303 aa overlap 2 3 8 10 [Reporter]SCO2921 (12N18)_PCR/4699 [Gene]SCO2921/4286 NC_003888 SCE19A.21, putative membrane protein, len: 99 aa; unknown function, probable CDS suggested by probable CDS suggested by GC frameplot and positional base preference. Contains possible hydrophobic membrane spanning regions 1 1 3 2 [Reporter]SCP1.249c (20E8)_PCR/45 [Gene]SCP1.249c/41 NC_003888 SCP1.249c, possible membrane protein, len: 109aa; contains possible membrane-spanning hydrophobic region. 2 3 8 9 [Reporter]SCO5168 (13J18)_PCR/4698 [Gene]SCO5168/4285 NC_003888 SCP8.31c, possible integral membrane protein, len: 89 aa. Contains possible hydrophobic membrane spanning regions. Also high content in alanine and leucine amino acid residues 1 1 2 22 [Reporter]SCO5358 (1E4)_PCR/43 [Gene]SCO5358/40 NC_003888 2SC6G5.02, possible membrane protein, len: 382 aa; regions similar to many of undefined function e.g. TR:O68907 (EMBL:AF058302) FrnA from Streptomyces roseofulvus (770 aa) fasta scores; opt: 747, z-score: 793.3, E(): 0, 40.8% identity in 304 aa overlap. Also similar to others from Streptomyces coelicolor e.g. TR:O69850 (EMBL:AL023702) putative transcriptional regulator (366 aa) fasta scores; opt: 1200, z-score: 1277.4, E(): 0, 52.2% identity in 356 aa overlap. Contains possible membrane-spanning hydrophobic regions 2 3 8 8 [Reporter]SCO1459 (14F18)_PCR/4697 [Gene]SCO1459/4284 NC_003888 SCL6.16c, possible amino acid transporter, len: 496 aa; similar to TR:CAB63187 (EMBL:AL133469) Streptomyces coelicolor putative amino acid transporter protein SCM10.26, 468 aa; fasta scores: opt: 1245 z-score: 1366.2 E(): 0; 44.7% identity in 463 aa overlap. Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease and possible hydrophobic membrane spanning regions 2 3 8 7 [Reporter]SCO0478 (15B18)_PCR/4696 [Gene]SCO0478/4283 NC_003888 SCF76.18c, possible membrane protein, len: 374 aa. Contains a possible membrane spanning hydrophobic domain. 2 3 8 6 [Reporter]SCO2975 (16N14)_PCR/4695 [Gene]SCO2975/4282 NC_003888 SCE50.03, unknown, len: 1345 aa. High G+C content (80.71%) and high also in alanine, glycine and proline amino acid residues 2 3 8 5 [Reporter]SCO0953 (17J14)_PCR/4694 [Gene]SCO0953/4281 NC_003888 SCM11.08, probable lacI-family transcriptional regulator, len: 349 aa; similar to TR:O86598 (EMBL:AL031514) Streptomyces coelicolor putative transcriptional regulator, SC2H4.15, 342 aa; fasta scores opt: 949 z-score: 1067.0 E(): 0; 44.6% identity in 341 aa overlap and to SW:ASCG_ECOLI (EMBL:M73326) Escherichia coli cryptic asc operon repressor, 336 aa; fasta scores: opt: 409 z-score: 463.9 E(): 1.8e-18; 28.4% identity in 334 aa overlap. Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family, score 37.90, E-value 3.1e-09 and PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family and Prosite match to entry PS00356 Bacterial regulatory proteins, lacI family signature. Contains also a possible helix-turn-helix motif at residues 11..32 (+5.76 SD) 1 1 20 12 [Reporter]SCO2613 (11E12)_PCR/429 [Gene]SCO2613/389 NC_003888 SCC88.24c, possible membrane protein, len: 117 aa; identical to previously sequenced TR:O06852 (EMBL:Y13070) Streptomyces coelicolor hypothetical 12.3 kD protein, 117 aa. Contains possible hydrophobic membrane spanning regions 2 3 8 4 [Reporter]SCO0867 (18F14)_PCR/4693 [Gene]SCO0867/4280 NC_003888 SCM2.20c, possible integral-membrane protein, len: 234 aa. Similar to Streptomyces coelicolor TR:CAB51458(EMBL:AL096884) putative membrane protein (236 aa), fasta scores opt: 211 z-score: 247.5 E(): 2.1e-06 28.8% identity in 243 aa overlap. Note this CDS appears to be a duplicated on this cosmid and shares 40.6 % identity, in 256 aa overlap, with the upstream gene, SCM2.18c. Contains a possible N-terminal signal sequence and possible membrane spanning hydrophobic domains. 1 1 20 11 [Reporter]SCO4181 (12A12)_PCR/428 [Gene]SCO4181/388 NC_003888 SCD66.18, conserved hypothetical protein, len: 321 aa; similar to TR:Q9RHX9 (EMBL:AB003158) Corynebacterium ammoniagenes ORF4, 359 aa; fasta scores: opt: 368 z-score: 441.2 E(): 4.2e-17; 38.9% identity in 319 aa overlap. Contains Pfam match to entry PF01571 GCV_T, Glycine cleavage T-protein (aminomethyl transferase) 1 1 20 10 [Reporter]SCO3098 (13M8)_PCR/427 [Gene]SCO3098/387 NC_003888 SCE41.07c, possible secreted protein, len: 341 aa; similar to TR:O53879 (EMBL:AL022004) Mycobacterium tuberculosis hypothetical 40.0 kDa protein MTV043.60c, 407 aa; fasta scores: opt: 443 z-score: 346.0 E(): 9.1e-12; 33.3% identity in 234 aa overlap. Contains Pfam match to entry PF01476 PG_binding_2, Putative peptidoglycan binding domain and high content in serine and glycine amino acid residues. Also contains a possible N-terminal region signal peptide sequence 1 1 20 9 [Reporter]SCO0346 (14I8)_PCR/426 [Gene]SCO0346/386 NC_003888 SCF41.05, possible 2-hydroxyhepta-2,4-diene-1,7- dioate isomerase, len: 292 aa. Similar to many e.g. Mycobacterium leprae TR:O33119 (EMBL; Z99263) possible 2-hydroxyhepta-2, 4-diene-1,7- dioate isomerase (242 aa), fasta scores opt: 491 z-score: 566.1 E(): 3.5e-24 40.6% identity in 212 aa overlap, Streptomyces coelicolor TR:O86527 (EMBL; AL031124) hypothetical 28.2 KD protein SC1C2.27 (261 aa), fasta scores opt: 465 z-score: 479.6 E(): 1.9e-21 40.0% identity in 220 aa overlap and to the C-terminus of Escherichia coli SW:HPCE_ECOLI (EMBL; X75028) homoprotocatechuate catabolism bifunctional isomerase/decarboxylase (405 aa), fasta scores opt: 479 z-score: 549.1 E(): 3.1e-23 42.8% identity in 187 aa overlap. Contains a Pfam match to entry PF01557 FAA_hydrolase. 1 1 20 8 [Reporter]SCO0239 (15E8)_PCR/425 [Gene]SCO0239/385 NC_003888 SCJ9A.18c, unknown, len: 357aa; 1 1 20 7 [Reporter]SCO1591 (16A8)_PCR/424 [Gene]SCO1591/384 NC_003888 SCI35.13, probable 3-hydroxyacyl-CoA dehydrogenase, len: 303 aa; similar to many e.g. SW:MMGB_BACSU probable 3-hydroxybutyryl-CoA dehydrogenase (287 aa), fasta scores; opt: 764 z-score: 1162.2 E(): 0, 45.6% identity in 283 aa overlap and SW:HBD_CLOAB 3-hydroxybutyryl-CoA dehydrogenase (282 aa), fasta scores; opt: 733 z-score: 1075.7 E(): 0, 43.6% identity in 282 aa overlap. Contains Pfam match to entry PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase, score 361.30, E-value 1.1e-104 1 1 20 6 [Reporter]SCO3124 (17M4)_PCR/423 [Gene]SCO3124/383 NC_003888 SCE66.03, ribosomal L25p family protein, len: 198 aa; similar to many members of the ribosomal L25p family (includes ribosomal protein, general stress proteins and glutaminyl-tRNA synthetases) eg. SW:P14194 (CTC_BACSU) general stress protein from Bacillus subtilis (203 aa) fasta scores; opt: 327, z-score: 393.9, E(): 1.8e-14, 31.6% identity in 196 aa overlap. Contains Pfam match to entry PF01386 Ribosomal_L25p, Ribosomal L25p family and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 1 20 5 [Reporter]SCO0847 (18I4)_PCR/422 [Gene]SCO0847/382 NC_003888 SCF43A.37c, possible lyase, len: 152 aa; weakly similar to many lactoylglutathione lyases e.g. SW:LGUL_SALTY (EMBL:U57364), GloA, Salmonella typhimurium lactoylglutathione lyase (135 aa), fasta scores; opt: 133 z-score: 175.2 E(): 0.02, 28.7% identity in 136 aa overlap. Weakly similar to SCI7.11c (EMBL:AL096743) S.coelicolor possible lyase (130 aa) (28.3% identity in 127 aa overlap). An alternative start codon is present at codon 3 1 1 20 4 [Reporter]SCP1.277 (19E4)_PCR/421 [Gene]SCP1.277/381 NC_003888 SCP1.277, unknown, doubtful CDS, len: 71aa; 1 1 20 3 [Reporter]SCO1666 (20A4)_PCR/420 [Gene]SCO1666/380 NC_003888 SCI52.08c, possible phosphatase, len: 228 aa; similar to SW:COBC_ECOLI (EMBL:U23163) Escherichia coli alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.*) CobC or PhpB or B0638, aa; fasta scores: opt: 212 Z-score: 252.7 bits: 53.8 E(): 1.7e-06; 29.412% identity in 187 aa overlap and to TR:O06240 (EMBL:Z95388) Mycobacterium tuberculosis hypothetical 21.5 kDa protein MTCY270.33, 250 aa; fasta scores: opt: 725 Z-score: 836.0 bits: 162.0 E(): 5.5e-39; 51.923% identity in 208 aa overlap. Contains Pfam match to entry PF00300 PGAM, Phosphoglycerate mutase family 3 4 2 8 [Reporter]SCO1200 (14O9)_PCR/7084 [Gene]SCO1200/6459 NC_003888 SCG11A.31c, probable regulatory protein, len 191 aa; similar to TR:Q54855 (EMBL:X61931) Streptomyces purpurascens ORF1, 90 aa; fasta scores: opt: 560 z-score: 686.7 E(): 7.2e-31; 85.6% identity in 90 aa overlap and to TR:CAB39703 (EMBL:AL049485) Streptomyces coelicolor putative DNA-binding protein SC6A5.19, 204 aa; fasta scores: opt: 382 z-score: 468.0 E(): 1.1e-18; 39.2% identity in 176 aa overlap and to Streptomyces coelicolor SC6G4.21, fasta scores: opt: 717 z-score: 721.2 E(): 8e-35; 60.0% identity in 190 aa overlap. Contains match to Pfam entry PF01381 HTH_3, Helix-turn-helix and a predicted possible motif at residues 25..46 (+6.09 SD) 3 4 2 7 [Reporter]SCO3911 (15K9)_PCR/7083 [Gene]SCO3911/6458 NC_003888 SCH24.33, dnaB, probable replicative DNA helicase, len: 491 aa; similar to many e.g. SW:DNAB_ECOLI (EMBL:K01174), DnaB, Escherichia coli replicative DNA helicase (471 aa), fasta scores; opt: 1144 z-score: 1233.0 E(): 0, 38.6% identity in 461 aa overlap. The position of the putative start codon is suggested with reference to GC frame plot and a potential RBS. Contains Pfam match to entry PF00772 DnaB, DnaB-like helicase, score 558.50, E-value 4.4e-164 and PS00017 ATP/GTP-binding site motif A (P-loop). Contains probable helix-turn-helix motif at aa 392-413 (Score 982, +2.53 SD) 3 4 2 6 [Reporter]SCO0177 (16G9)_PCR/7082 [Gene]SCO0177/6457 NC_003888 SCJ1.26, possible membrane protein, len: 193 aa. Similar to Mycobacterium tuberculosis TR:P71991(EMBL; Z81360) hypothetical 22.5 KD protein (210 aa), fasta scores opt: 287 z-score: 333.1 E(): 3.4e-11 35.2% identity in 182 aa overlap and S. coelicolor TR:Q9ZBP2 (EMBL;AL034492) 50KD proline rich protein (456 aa), fasta scores opt: 338 z-score: 385.2 E(): 4.3e-14 35.6% identity in 188 aa overlap. Contains possible membrane-spanning hydrophobic region. 3 4 2 5 [Reporter]SCO1054 (17C9)_PCR/7081 [Gene]SCO1054/6456 NC_003888 SCG20A.34, possible aminotransferase, len: 400 aa; similar to TR:Q9XAD5 (EMBL:AL096839) Streptomyces coelicolor putative aminotransferase SCC22.03c, 418 aa; fasta scores: opt: 397 z-score: 446.1 E(): 2.3e-17; 27.7% identity in 423 aa overlap and to TR:Q9RY03 (EMBL:AE001877) Deinococcus radiodurans NifS-related protein DR0151, 401 aa; fasta scores: opt: 791 z-score: 884.2 E(): 0; 41.2% identity in 396 aa overlap. Contains Pfam match to entry PF00266 aminotran_5, Aminotransferases class-V 1 1 2 21 [Reporter]SCO0770 (2A4)_PCR/42 [Gene]SCO0770/39 NC_003888 3SCF60.02c, possible membrane protein, len: 185 aa. Contains possible hydrophobic membrane spanning region 3 4 2 4 [Reporter]SCO0112 (18O5)_PCR/7080 [Gene]SCO0112/6455 NC_003888 SCJ11.41c, improbable CDS, function unknown, len: 51aa; predicted by GC Frameplot. 1 1 2 20 [Reporter]SCO4602 (2M24)_PCR/41 [Gene]SCO4602/38 NC_003888 SCD39.02, nuoH2, NADH dehydrogenase subunit, len: 322 aa; similar to SW:NUOH_ECOLI (EMBL:X68301) Escherichia coli NADH dehydrogenase I chain H (EC 1.6.5.3) NuoH, 325 aa; fasta scores: opt: 715 z-score: 812.7 E(): 0; 37.5% identity in 317 aa overlap and to TR:Q9XAR1 (EMBL:AL078618) Streptomyces coelicolor SCD16A.14c NuoH, NADH dehydrogenase subunit, 467 aa; fasta scores: opt: 444 z-score: 437.6 E(): 9.7e-19; 37.9% identity in 335 aa overlap. Contains Pfam match to entry PF00146 NADHdh, NADH dehydrogenases and possible hydrophobic membrane spanning regions 3 4 2 3 [Reporter]SCP1.198 (19K5)_PCR/7079 [Gene]SCP1.198/6454 NC_003888 SCP1.198, unknown, len: 302aa; Note possible alternative downstream translational start sites. 1 1 2 19 [Reporter]SCO6146 (3I24)_PCR/40 [Gene]SCO6146/37 NC_003888 SC1A9.10, unknown, len: 174 aa 3 4 2 2 [Reporter]SCO1881 (20C5)_PCR/7078 [Gene]SCO1881/6453 NC_003888 SCI39.28c, putative secreted protein, len: 267 aa; similar to TR:O31528 (EMBL:Z99107) Bacillus subtilis YesY protein, 217 aa; fasta scores: opt: 548 Z-score: 648.8 bits: 127.5 E(): 1.5e-28; 43.939% identity in 198 aa overlap and to TR:Q9L936 (EMBL:AF184175) Bacillus sp. KCCM10143 cephalosporin C deacetylase 217 aa; fasta scores: opt: 544 Z-score: 644.2 bits: 126.6 E(): 2.7e-28; 44.500% identity in 200 aa overlap. Contains possible N-terminal region signal peptide sequence 1 1 2 18 [Reporter]SCO6203 (4E24)_PCR/39 [Gene]SCO6203/36 NC_003888 SC2G5.24c, unknown, len: 112aa 3 4 1 22 [Reporter]SCO5179 (1C1)_PCR/7076 [Gene]SCO5179/6452 NC_003888 2SC3B6.03c, peptidase, len: 513 aa; identical to TR:Q54399 (EMBL:L42759) Streptomyces lividans proteinase SlpE, 513 aa and highly similar to Streptomyces coelicolor 2SC3B6.04c, 516 aa; fasta scores: opt: 1544 z-score: 1384.8 E(): 0; 50.2% identity in 526 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 1 1 2 17 [Reporter]SCO6556 (5A24)_PCR/38 [Gene]SCO6556/35 NC_003888 SC4B5.06, possible transmembrane protein, similar to SW:YFEH_ECOLI Hypothetical protein from Escherichia coli, (332 aa), fasta scores; opt: 985, z-score: 1173.2, E(): 0, (48.9% identity in 323 aa overlap). Hydrophobic with several possible membrane spanning regions. 2 3 8 3 [Reporter]SCO6320 (19B14)_PCR/4692 [Gene]SCO6320/4279 NC_003888 SCIF3.22c, possible transport integral membrane protein, len: 589 aa; similar to TR:O28857 (EMBL:AE001006) Archaeoglobus fulgidus chloride channel, putative AF1415, 589 aa; fasta scores: opt: 941 z-score: 956.9 E(): 0; 34.4% identity in 576 aa overlap. Contains Pfam match to entry PF00654 voltage_CLC, Voltage gated chloride channels and 2x PF00571 CBS, CBS domain. Also contains possible hydrophobic membrane spanning regions 3 4 1 21 [Reporter]SCO1757 (1O21)_PCR/7075 [Gene]SCO1757/6451 NC_003888 2SCI34.10, unknown, len: 97 aa 1 1 2 16 [Reporter]SCO7307 (6M20)_PCR/37 [Gene]SCO7307/34 NC_003888 SC5F8.17c, possible LysR-family transcriptional regulator, len: 300 aa. Similar to many other LysR-family proteins including: Corynebacterium glutamicum SW:LYSG_CORGL (EMBL:X96471) lysine export transcriptional regulatory protein, LysG (290 aa), fasta scores opt: 780 z-score: 918.3 E():0 44.5% identity in 290 aa overlap and Escherichia coli SW:ICIA_ECOLI (EMBL:M62865) chromosome initiation inhibitor, IciA (297 aa), fasta scores opt: 538 z-score: 635.6 E(): 6.2e-28 37.1% identity in 291 aa overlap. Contains a Prosite hit to PS00044 Bacterial regulatory proteins, lysR family signature and a Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family with the putative helix-turn-helix motif situated between residues 23..44 (+4.82 SD). 2 3 8 2 [Reporter]SCO0063 (20J10)_PCR/4691 [Gene]SCO0063/4278 NC_003888 SCJ4.44c, possible glucokinase, len: 311 aa; similar to many eg. SW:GLK_STRCO glucokinase from Streptomyces coelicolor (317 aa) fasta scores; opt: 175, z-score: 195.7, E(): 0.0015, (28.2% identity in 326 aa overlap). Contains Pfam match to entry PF00480 ROK, ROK family 3 4 1 20 [Reporter]SCO0216 (2K21)_PCR/7074 [Gene]SCO0216/6450 NC_003888 SCJ12.28, narG2, probable nitrate reductase alpha chain, len: 1233 aa. Highly similar to Streptomyces coelicolor TR:O86717 (EMBL; AL031515) putative nitrate reductase alpha chain NarG SC5C7.20c (1231 aa), fasta scores: opt: 5792 z-score: 6544.7 E(): 0 67.6% identity in 1230 aa overlap and Bacillus subtilis SW:NARG_BACSU (EMBL; Z49884) nitrate reductase alpha chain (EC 1.7.99.4) NarG (1228 aa), fasta scores: opt: 4331 z-score: 4891.8 E(): 0 52.9% identity in 1201 aa overlap. Contains a PS00551 Prokaryotic molybdopterin oxidoreductases signature 1, a Pfam match to entry PF00384 molybdopterin, Prokaryotic molybdopterin oxidoreductases and a Pfam match to entry PF01568 Molydop_binding, Prokaryotic molybdopterin oxidoreductases. 1 1 2 15 [Reporter]SCO4418 (7I20)_PCR/36 [Gene]SCO4418/33 NC_003888 SC6F11.16c, probable oxidase, len: 222 aa; similar to TR:O88794 (EMBL:U91561) Rattus norvegicus pyridoxine 5'-phosphate oxidase PnpO, 261 aa; fasta scores: opt: 413 z-score: 479.7 E(): 3.2e-19; 38.0% identity in 184 aa overlap. Contains Pfam match to entry PF01243 Pyridox_oxidase, Pyridoxamine 5'-phosphate oxidase 2 3 7 22 [Reporter]SCO4214 (1J6)_PCR/4689 [Gene]SCO4214/4277 NC_003888 2SCD46.28c, possible AbaA-like regulatory protein, len: 146aa; similar to many from Streptomyces coelicolor eg. TR:Q53897 (EMBL:X60316) AbaA pleiotropic regulatory protein (192 aa) fasta scores; opt: 150, z-score: 206.3, E(): 0.0005, 30.7% identity in 140 aa overlap and TR:CAB76014 (EMBL:AL157916) putative AbaA-like regulatory protein (220 aa) fasta scores; opt: 159, z-score: 217.2, E(): 0.00012, 37.4% identity in 131 aa overlap. 1 1 2 14 [Reporter]SCO6881 (8E20)_PCR/35 [Gene]SCO6881/32 NC_003888 SC7F9.33c, possible ATP-binding protein, len: 462 aa. Highly similar to Streptomyces coelicolor TR:Q9X870(EMBL:AL049661) putative ATP-binding protein, SCE134.15 (469 aa), fasta scores opt: 1469 z-score: 1094.9 E():0 52.2% identity in 490 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). Contains possible membrane spanning hydrophobic domains. 2 3 7 21 [Reporter]SCO5932 (2F6)_PCR/4688 [Gene]SCO5932/4276 NC_003888 SC7H1.02, abfB, arabinofuranosidase precursor, len: 475 aa; almost identical to S. lividans TR:P96463 (EMBL:M64551) arabinofuranosidase precursor (EC 3.2.1.55)(alpha-l-arabinofuranosidase) (arabinosidase) (478 aa), fasta scores; opt: 3129 z-score: 1807.5 E(): 0, 95.8% identity in 477 aa overlap. Contains N-terminal signal sequence 1 1 2 13 [Reporter]SCO7159 (9A20)_PCR/34 [Gene]SCO7159/31 NC_003888 SC9A4.21, possible integral membrane protein, len: 645 aa; similar to TR:O32225 (EMBL:Z99121) Bacillus subtilis YvaC protein, 631 aa; fasta scores: opt: 402 z-score: 435.7 E(): 8.4e-17; 23.8% identity in 627 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 7 19 [Reporter]SCO6493 (4N2)_PCR/4686 [Gene]SCO6493/4275 NC_003888 SC1E6.02c, unknown, len: 115 aa 1 1 2 12 [Reporter]SCO1944 (10M16)_PCR/33 [Gene]SCO1944/30 NC_003888 SCC54.04c, secG, integral membrane protein, len: 102 aa; varying levels of similarity to many with similarity to SecG proteins e.g. TR:E1363411 (EMBL:AJ007732) SecG protein (undefined) from Corynebacterium glutamicum (77 aa) fasta scores; opt: 287, z-score: 358.8, E(): 1.1e-12, 53.4% identity in 73 aa overlap) and SW:SECG_ECOLI SecG, membrane protein involved in protein translocation across the cytoplasmic membrane, (110 aa) fasta scores; opt: 118, z-score: 157.8, E(): 0.17, (26.2% identity in 65 aa overlap). Contains possible membrane spanning hydrophobic regions 2 3 7 18 [Reporter]SCO6844 (5J2)_PCR/4685 [Gene]SCO6844/4274 NC_003888 SC3D9.12c, possible DNA methylase, len: 423 aa. Weakly similar to Salmonella infantis SW:MTSI_SALIN(EMBL:J03391) modification methylase, SinI (EC 2.1.1.73) (461 aa), fasta scores opt: 244 z-score: 269.9 E(): 1.3e-07 24.5% identity in 355 aa overlap. Contains 2xPfam matches to entries PF00145 DNA_methylase, C-5 cytosine-specific DNA methylase. 2 3 7 17 [Reporter]SCO6629 (6F2)_PCR/4684 [Gene]SCO6629/4273 NC_003888 SC4G2.03, hypothetical protein, len: 277 aa; similar to TR:Q9F345 (EMBL:AL391751) Streptomyces coelicolor hypothetical protein SC9E12.10c, 287 aa; fasta scores: opt: 769 Z-score: 890.8 E(): 5.5e-42; 48.736% identity in 277 aa overlap 1 2 13 21 [Reporter]SCO2390 (2M11)_PCR/2300 [Gene]SCO2390/2099 NC_003888 SC4A7.18, fabF, 3-oxoacyl-[acyl-carrier-protein] synthase II, len: 429 aa; highly similar to the previously sequenced TR:Q54208 (EMBL:L43074) Streptomyces glaucecens FabB; fasta scores: opt: 2527 z-score: 2640.7 E(): 0; 89.7% identity in 429 aa overlap and similar to S. coelicolor SCF11.27c, 428 aa; fasta scores: opt: 1391 z-score: 1224.9 E(): 0; 53.1% identity in 422 aa overlap and to S. coelicolor SC3F7.06c, 407 aa; fasta scores: opt: 884 z-score: 780.9 E(): 0; 41.9% identity in 427 aa overlap. Contains Pfam match to entry PF00109 ketoacyl-synt, Beta-ketoacyl synthase 2 3 7 16 [Reporter]SCO0302 (7B2)_PCR/4683 [Gene]SCO0302/4272 NC_003888 SC5G9.11c, possible tetR-family transcriptional regulator, len: 197 aa; shows weak similarity to A-factor receptors and their homologues e.g. TR:O66121 (EMBL:AB000384), CprA, Streptomyces coelicolor A-factor receptor homologue involved in the regulation of secondary metabolism and morphogenesis (215 aa), fasta scores; opt: 168 z-score: 202.3 E(): 0.00066, 30.1% identity in 193 aa overlap. Contains probable helix-turn-helix motif at aa 43-64 (Score 1686, +4.93 SD). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 1 2 13 19 [Reporter]SCO6019 (4E11)_PCR/2298 [Gene]SCO6019/2098 NC_003888 SC1C3.07, unknown, len: 373 aa; similar to upstream gene SC1C3.06 (E(): 2.1e-21, 36.2% identity in 348 aa overlap) and to C-terminus of SC1C3.13 (E(): 1.8e-18, 31.8% identity in 337 aa overlap) 2 3 7 15 [Reporter]SCO6965 (7N22)_PCR/4682 [Gene]SCO6965/4271 NC_003888 SC6F7.18c, unknown, len: 110 aa. Similar to Streptomyces coelicolor TR:Q9XA81(EMBL:AL096837) possible DNA-binding protein, SCF43A.34C (113 aa), fasta scores opt: 235 z-score: 310.8 E(): 7.5e-10 40.4% identity in 109 aa overlap. 1 1 19 22 [Reporter]SCO1354 (1I20)_PCR/417 [Gene]SCO1354/379 NC_003888 2SCG61.36c, possible secreted protein, len: 187aa; contains a possible N-terminal signal sequence and Prosite match to PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. 1 2 13 18 [Reporter]SCO5449 (5A11)_PCR/2297 [Gene]SCO5449/2097 NC_003888 SC3D11.06c, probable ABC transporter, len: 605aa; similar to many eg. TR:Q54203 (EMBL:X89010) from the 5'-hydroxystreptomycin production/transport operon of Streptomyces glaucescens (584 aa) fasta scores; opt: 885, z-score: 878.4, E(): 0, 40.3% identity in 601 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, Pfam match to entry PF00664 ABC_membrane, ABC transporter transmembrane region and Prosite match to PS00211 ABC transporters family signature. 2 3 7 14 [Reporter]SCO7193 (8J22)_PCR/4681 [Gene]SCO7193/4270 NC_003888 SC8A11.21, conserved hypothetical protein, len: 316 aa; similar to TR:O07180 (EMBL:Z96797) Mycobacterium tuberculosis hypothetical 36.0 kDa protein MTCY07H7A.10, 355 aa; fasta scores: opt: 1256 z-score: 1437.6 E(): 0l; 60.9% identity in 307 aa overlap 1 1 19 21 [Reporter]SCO0655 (2E20)_PCR/416 [Gene]SCO0655/378 NC_003888 SCF91.15, gvpJ2, possible gas vesicle synthesis protein, len: 114 aa. Highly similar to many other gas vesicle synthesis proteins including: Bacillus megaterium TR:O6869(EMBL:AF053765) gas vesicle protein GvpJ (100 aa), fasta scores opt: 337 z-score: 453.5 E(): 7e-18 58.8% identity in 85 aa overlap and Streptomyces coelicolor TR:Q9ZC08 (EMBL:AL033505) putative gas vesicle synthesis protein GvpJ (109 aa), fasta scores opt: 423 z-score: 564.4 E(): 4.6e-24 69.8% identity in 96 aa overlap. Contains a Pfam match to entry PF00741 Gas_vesicle, Gas vesicle protein and a Prosite hit to PS00234 Gas vesicles protein GVPa signature 1. Also contains a probable coiled-coil from 78 to 114 (37 residues). 1 2 13 17 [Reporter]SCO5832 (6M7)_PCR/2296 [Gene]SCO5832/2096 NC_003888 SC5B8.22, putative citrate synthase, len: 390 aa ; similar to citrate synthase from many organisms e.g. CISZ _BACSU P39120 bacillus subtilis. citrate synthase i (372 aa ), fasta scores; opt: 729 z-score: 877.4 E(): 0, 35.5% iden tity in 372 aa overlap. Contains PS00480 Citrate synthase s ignature and Pfam match to entry citrate_synt PF00285, Citr ate synthase, score 314.55. Also similar to downstream gene SC5B8.21c (421 aa) E(): 2.6e-14, 32.4% identity in 352 aa overlap 1 1 19 20 [Reporter]SCO7664 (3A20)_PCR/415 [Gene]SCO7664/377 NC_003888 SC10F4.37c, conserved hypothetical protein, len: 178 aa; similar to TR:Q9Y9P2 (EMBL:AP000064) Aeropyrum pernix hypothetical 20.8 kDa protein APE2246, 188 aa; fasta scores: opt: 318 z-score: 401.6 E(): 7.8e-15; 35.7% identity in 157 aa overlap 1 2 13 16 [Reporter]SCO6758 (7I7)_PCR/2295 [Gene]SCO6758/2095 NC_003888 SC6A5.07, probable ABC transporter ATP-binding component, len: 253aa; similar to many eg. SW:TAGH_BACSU TagH, ABC transporter ATP-binding component from Bacillus subtilis (527 aa) fasta scores; opt: 621, z-score: 686.7, E(): 6e-31, (48.5% identity in 202 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, score 123.70, E-value 3.4e-33 and Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. 1 1 19 19 [Reporter]SCO7226 (4M16)_PCR/414 [Gene]SCO7226/376 NC_003888 SC2H12.25, possible integral membrane protein, len: 146 aa. Similar to several proteins of unproven function e.g. Streptomyces coelicolor TR:Q9X9Z5(EMBL:AL079332) putative integral membrane protein, SCI5.05C (143 aa), fasta scores opt: 321 z-score: 398.2 E(): 1.1e-1437.4% identity in 139 aa overlap. Contains possible membrane spanning hydrophobic domains. 1 2 13 15 [Reporter]SCO4764 (8E7)_PCR/2294 [Gene]SCO4764/2094 NC_003888 SC6G4.42c, probable dehydrogenase, len: 257 aa; simi lar to many members of the short-chain dehydrogenases/reduc tases family e.g. TR:Q54190 (EMBL:D14040) S. galilaeus keto acyl reductase aknIII (261 aa), fasta scores; opt: 350 z-sc ore: 438.4 E(): 3.5e-17, 33.5% identity in 233 aa overlap. Contains Pfam match to entry PF00106 adh_short, Alcohol/oth er dehydrogenases, short chain type, score 158.00, E-value 1.6e-43 1 1 19 18 [Reporter]SCO7688 (5I16)_PCR/413 [Gene]SCO7688/375 NC_003888 SC4C2.23, hypothetical protein, len: 242 aa; similar to TR:CAC04224 (EMBL:AL391515) Streptomyces coelicolor hypothetical 27.9 kDa protein SC4B10.19c, 254 aa; fasta scores: opt: 222 z-score: 264.0 E(): 3.6e-07; 31.8% identity in 214 aa overlap and to Streptomyces coelicolor hypothetical protein SC4C2.20, 677 aa; fasta scores: opt: 705 z-score: 683.1 E(): 2.2e-32; 49.8% identity in 243 aa overlap 1 2 13 14 [Reporter]SCO7736 (9A7)_PCR/2293 [Gene]SCO7736/2093 NC_003888 SC8D11.27c, possible integral membrane protein, len: 753 aa; similar to TR:Q9K4J6 (EMBL:AL357613) Streptomyces coelicolor hypothetical 79.6 kDa protein SC5F8.28c, 752 aa; fasta scores: opt: 773 z-score: 865.8 E(): 0; 37.3% identity in 743 aa overlap. Contains 10 possible hydrophobic membrane spanning regions 1 1 19 17 [Reporter]SCO7780 (6E16)_PCR/412 [Gene]SCO7780/374 NC_003888 SC5E9.28, possible transcriptional regulator, len: 313aa; strongly similar to TR:Q54308 (EMBL:X86780) helix-turn-helix DNA binding protein from Streptomyces hygroscopicus (330 aa) fasta scores; opt: 921, z-score: 1007.9, E(): 0, 50.5% identity in 309 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family and Prosite match to PS00041 Bacterial regulatory proteins, araC family signature. 1 2 13 13 [Reporter]SCO6269 (10M3)_PCR/2292 [Gene]SCO6269/2092 NC_003888 SCAH10.34c, possible oxidoreductase beta-subunit, 350 aa; highly similar to TR:O53181 (EMBL:AL021246) Mycobacterium tuberculosis oxidoreductase beta-subunit, 373 aa; fasta scores: opt: 1415 z-score: 1622.6 E(): 0; 64.0% identity in 342 aa overlap. Contains possible N-terminal region signal sequence 1 1 19 16 [Reporter]SCO6175 (7A16)_PCR/411 [Gene]SCO6175/373 NC_003888 SC6C5.11, hypothetical protein, len: 327 aa; similar to TR:Q9LAB8 (EMBL:AF144381) Burkholderia gladioli esterase EstC, 298 aa; fasta scores: opt: 532 Z-score: 590.8 E(): 2.8e-25; 38.014% identity in 292 aa overlap 1 2 13 12 [Reporter]SCO2361 (11I3)_PCR/2291 [Gene]SCO2361/2091 NC_003888 SCC8A.19c, unknown, len: 270aa; 1 1 19 15 [Reporter]SCO5758 (8M12)_PCR/410 [Gene]SCO5758/372 NC_003888 SC7C7.13, possible transcriptional regulator, len: 124 aa; similar to several putative transcriptional regulators fomr M. tuberculosis e.g. TR:O53478 (EMBL:AL021899) putative regulator MTV018.21 (Rv2034) (107 aa), fasta scores; opt: 178 z-score: 327.3 E(): 5.4e-11, 40.7% identity in 81 aa overlap. Contains probable helix-turn-helix motif at aa 34-55 (Score 999, +2.59 SD) 1 2 13 11 [Reporter]SCO4778 (12E3)_PCR/2290 [Gene]SCO4778/2090 NC_003888 SCD63.10, pkaI, serine/threonine protein kinase, len: 380 aa; similar to TR:CAB50939 (EMBL:AL096849) Streptomyces coelicolor putative protein serine/threonine kinase SCI11.13, 550 aa; fasta scores: opt: 932 z-score: 751.0 E(): 0; 45.0% identity in 416 aa overlap and to SW:PKAA_STRCO (EMBL:D86821) Streptomyces coelicolor serine/threonine protein kinase PkaA (EC 2.7.1.-), 543 aa; fasta scores: opt: 557 z-score: 454.6 E(): 6.8e-18; 33.9% identity in 386 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and matches to Prosite entries PS00107 Protein kinases ATP-binding region signature and possible hydrophobic membrane spanning region 1 1 19 14 [Reporter]SCO5402 (9I12)_PCR/409 [Gene]SCO5402/371 NC_003888 SC8F4.06c, Asp-rich hydrophilic protein (putative membrane protein), len: 257 aa. Contains possible hydrophobic membrane spanning region 1 1 19 13 [Reporter]SCO6242 (10E12)_PCR/408 [Gene]SCO6242/370 NC_003888 SCAH10.07, hypothetical protein, len: 304 aa; similar to TR:Q9RCK7 (EMBL:AJ242827) Streptomyces tendae hypothetical protein (fragment) 266 aa; fasta scores: opt: 739 Z-score: 852.1 E(): 7.9e-40; 54.435% identity in 248 aa overlap 3 4 1 19 [Reporter]SCO7385 (3G21)_PCR/7073 [Gene]SCO7385/6449 NC_003888 SC10G8.12, possible integral membrane protein, len: 147 aa. Contains possible membrane spanning hydrophobic domains. 3 4 1 18 [Reporter]SCO5870 (4C21)_PCR/7072 [Gene]SCO5870/6448 NC_003888 SC2E9.11, probable ABC-transporter ATP binding protein, len: 230 aa; highly similar to many eg. Y796_METJA Q58206 hypothetical abc transporter atp-binding protein (235 aa), fasta scores; opt: 666 z-score: 870.6 E(): 0, 43.8% identity in 226 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature and Pfam match to entry ABC_tran PF00005, ABC transporters, score 54.33 3 4 1 17 [Reporter]SCO6701 (5O17)_PCR/7071 [Gene]SCO6701/6447 NC_003888 SC4C6.11c, pcaF, beta-ketoadipyl-CoA thiolase, len: 400 aa; highly similar to pcaF (EMBL:AF117893) Streptomyces sp. 2065 beta-ketoadipyl-CoA thiolase (400 aa), fasta scores; opt: 2314 z-score: 2497.6 E(): 0, 87.4% identity in 397 aa overlap. Highly similar others from S.coelicolor e.g. (EMBL:AL049587), SC5F2A.14, S.coelicolor probable thiolase (404 aa) (40.2% identity in 423 aa overlap). Contains Pfam match to entry PF00108 thiolase, Thiolase, PS00099 Thiolases active site, PS00098 Thiolases acyl-enzyme intermediate signature and PS00737 Thiolases signature 2 3 4 1 16 [Reporter]SCO2173 (6K17)_PCR/7070 [Gene]SCO2173/6446 NC_003888 SC5F7.28, putative adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase, len: 397 aa; regions similar to different proteins. 1-220aa similar to TR:O05479 (EMBL:M86351) triacylglycerol acylhydrolase from Streptomyces sp. (481 aa) fasta scores; opt: 146, z-score: 158.6, E(): 0.17, (30.4% identity in 207 aa overlap). 220-397aa similar to many bifunctional adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase proteins in the biosynthesis of cobalamin (vitamin B12) eg. SW:COBU_SALTY adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium (181 aa) fasta scores; opt: 368, z-score: 398.3, E(): 7.5e-15, (41.7% identity in 175 aa overlap). 1 1 2 11 [Reporter]SCO4513 (11I16)_PCR/32 [Gene]SCO4513/29 NC_003888 SCD35.20c, unknown, len: 222 aa 3 4 1 15 [Reporter]SCO2666 (7G17)_PCR/7069 [Gene]SCO2666/6445 NC_003888 SC6D10.09, possible serine/threonine protein kinase, len: 903 aa; similar to TR:Q9ZBL8 (EMBL:AL035159) Mycobacterium leprae putative serine/threonine kinase MLCB1450.19c, 763 aa; fasta scores: opt: 1045 z-score: 821.3 E(): 0; 42.0% identity in 793 aa overlap and to SW:PKN2_MYXXA (EMBL:M94857) Myxococcus xanthus serine/threonine-protein kinase Pkn2 (EC 2.7.1.-), 830 aa; fasta scores: opt: 301 z-score: 243.3 E(): 3.8e-06; 28.9% identity in 433 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 1 1 2 10 [Reporter]SCO4270 (12E16)_PCR/31 [Gene]SCO4270/28 NC_003888 SCD95A.03c, possible transcriptional regulator, len: 199 aa; similar to TR:CAB53122 (EMBL:AL109962) Streptomyces coelicolor putative transcriptional regulatory protein SCJ1.04, 207 aa; fasta scores: opt: 154 z-score: 202.7 E(): 0.00079; 36.4% identity in 77 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 3 4 1 14 [Reporter]SCO6854 (8C17)_PCR/7068 [Gene]SCO6854/6444 NC_003888 SC7F9.06c, unknown, len: 267 aa. Similar to other hypothetical proteins predicted to be encoded by CDS located on this cosmid: SC7F9.02c (259 aa), fasta scores opt: 385 z-score: 396.2 E(): 1.3e-16 34.6% identity in 257 aa overlap and SC7F9.04c (273 aa), fasta scores opt: 376 z-score: 386.8 E(): 4.3e-16 30.5% identity in 272 aa overlap. 1 1 2 9 [Reporter]SCO3884 (13A16)_PCR/30 [Gene]SCO3884/27 NC_003888 SCH24.06, conserved hypothetical protein, len: 170 aa; previously sequenced as TR:O54595 (EMBL:Y16311) Streptomyces coelicolor jag-like protein (170 aa). Similar to SW:JAG_BACSU (EMBL:Z14225), jag, Bacillus subtilis SpoIIIJ associated protein (208 aa), fasta scores; opt: 231 z-score: 268.4 E(): 1.1e-07, 31.7% identity in 139 aa overlap, and other jag homologs e.g. TR:O53598 (EMBL:AL021426) Mycobacterium tuberculosis hypothetical protein (187 aa) (62.7% identity in 169 aa overlap) 3 4 1 13 [Reporter]SCO5844 (9O13)_PCR/7067 [Gene]SCO5844/6443 NC_003888 SC9B10.11, unknown, len: 397 aa 1 1 2 8 [Reporter]SCO1642 (14M12)_PCR/29 [Gene]SCO1642/26 NC_003888 SCI41.25, putative LacI family regulator, len 336 aa. Identical to TR:O87600 (EMBL:AF086832) Streptomyces coelicolor ORF4 and similar to TR:AAD36030 (EMBL:AE001758) Thermotoga maritima TM0949 transcriptional regulator, LacI family, 332 aa; fasta scores: opt: 476 z-score: 517.4 E(): 1.9e-21; 29.5% identity in 339 aa overlap. Contains possible helix-turn-helix motif at aa 23-44.(Score 1430, +4.06 SD) 3 4 1 12 [Reporter]SCO2319 (10K13)_PCR/7066 [Gene]SCO2319/6442 NC_003888 SCC53.10, possible tetR-family regulatory protein, len: 250 aa; regions similar to many proposed regulatory proteins e.g. TR:P96856 (EMBL:Z92774) hypothetical protein from Mycobacterium tuberculosis (199 aa) fasta scores; opt: 492, z-score: 572.6, E(): 2e-24, 44.8% identity in 183 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. 1 1 2 7 [Reporter]SCO1449 (15I12)_PCR/28 [Gene]SCO1449/25 NC_003888 SCL6.06c, hypothetical protein, len: 152 aa; similar to SW:YBGC_ECOLI (EMBL:M16489) Escherichia coli hypothetical 15.6 kD protein in CydB-TolQ intergenic region YbgC, 134 aa; fasta scores: opt: 140 z-score: 193.8 E(): 0.0023; 26.7% identity in 116 aa overlap 2 3 7 13 [Reporter]SCO6060 (9F22)_PCR/4680 [Gene]SCO6060/4269 NC_003888 SC9B1.07, murC, possible UDP-N-acetylmuramoyl-L-alanine ligase, len: 485 aa; similar to many e.g. SW:MURC_ECOLI UDP-N-acetylmuramoyl-L-alanine ligase from Escherichia coli (491 aa) fasta scores; opt: 750, z-score: 785.9, E(): 0, (40.4% identity in 463 aa overlap). 3 4 1 11 [Reporter]SCO4550 (11G13)_PCR/7065 [Gene]SCO4550/6441 NC_003888 SCD16A.33c, conserved hypothetical protein, len: 399 aa; similar to many e.g. TR:O25370 (EMBL:AE000579) conserved hypothetical protein from Helicobacter pylori (383 aa) fasta scores; opt: 1039, z-score: 1256.5, E(): 0, (44.5% identity in 357 aa overlap). 1 1 2 6 [Reporter]SCO4650 (16E12)_PCR/27 [Gene]SCO4650/24 NC_003888 SCD82.21, yrj1, possible lipoprotein, len: aa; identical to C-terminal of previously sequenced SW:YRJ1_STRCO (EMBL:L24552) Streptomyces coelicolor hypothetical protein in rplJ 5' region (ORF1) (fragment), 256 aa. Contains correctly situated match ot Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal signal peptide sequence 2 3 7 12 [Reporter]SCO2427 (10B22)_PCR/4679 [Gene]SCO2427/4268 NC_003888 SCC42.08c, probable secreted arabinase, len: 322 aa; similar to SW:ABNA_ASPNG (EMBL:L23430) Aspergillus niger arabinan endo-1,5-alpha-L-arabinosidase A precursor (EC 3.2.1.99) AbnA, 321 aa; fasta scores: opt: 764 z-score: 832.4 E(): 0; 42.0% identity in 326 aa overlap. Contains possible N-terminal region signal peptide sequence 3 4 1 10 [Reporter]SCO4233 (12C13)_PCR/7064 [Gene]SCO4233/6440 NC_003888 SCD8A.06, ispD, 4-diphodphocytidyl-2C-methyl-D-erythriol synthase, len: 270 aa; similar to many isoprenoid biosynthesis related proteins, e.g. SW:ISPD_ECOLI (EMBL:AF230736) Escherichia coli 4-diphodphocytidyl-2C-methyl-D-erythriol synthase (EC 2.7.7.*) IspD or B2747, 235 aa; fasta scores: opt: 304 Z-score: 307.9 E(): 1.6e-09; 34.632% identity in 231 aa overlap. Contains Pfam match to entry PF01128 UPF0007, Uncharacterized protein family UPF0007 and match to Prosite entry PS01295 Uncharacterized protein family UPF0007 signature 1 1 2 5 [Reporter]SCO3147 (17A12)_PCR/26 [Gene]SCO3147/23 NC_003888 SCE66.26c, possible ABC transporter ATP-binding subunit, len: 604aa; similar to many others eg. TR:O06476 (EMBL:D86418) from Bacillus subtilis (629 aa) fasta scores; opt: 1223, z-score: 1292.7, E(): 0, 37.0% identity in 638 aa overlap. Contains two Pfam matches to entry PF00005 ABC_tran, ABC transporter, three Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop) and two Prosite matches to PS00211 ABC transporters family signature. Also contains possible coiled-coil region 544-604aa. 2 3 7 11 [Reporter]SCO4612 (11N18)_PCR/4678 [Gene]SCO4612/4267 NC_003888 SCD39.12c, possible amino acid transporter, len: 414 aa; similar to SW:YJ99_MYCTU (EMBL:Z74025) Mycobacterium tuberculosis hypothetical 45.3 kDa transport protein MTVY39.19, 440 aa; fasta scores: opt: 1985 z-score: 2014.9 E(): 0; 79.0% identity in 404 aa overlap. Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease and possible hydrophobic membrane spanning regions 1 1 2 4 [Reporter]SCO5876 (18M8)_PCR/25 [Gene]SCO5876/22 NC_003888 SC2E9.17c, trkA, potassium uptake protein, len: 223 aa; similar to first domain of TRKA_ECOLI P23868 Trk system potassium uptake protein (458 aa), fasta scores; opt: 249 z-score: 389.9 E(): 1.6e-14, 28.6% identity in 227 aa overlap. Also similar to downstream ORF SC2E9.16c (E(): 0.00062, 22.6% identity in 212 aa overlap) 2 3 7 10 [Reporter]SCO2917 (12J18)_PCR/4677 [Gene]SCO2917/4266 NC_003888 SCE19A.17, conserved hypothetical protein, len: 448 aa; similar to hypothetical proteins from bacteria e.g. SW:Y03F_MYCTU (EMBL:Z73902) Mycobacterium tuberculosis hypothetical protein (509 aa), fasta scores; opt: 1488 z-score: 1661.8 E(): 0, 57.1% identity in 443 aa overlap 1 1 2 3 [Reporter]SCO6306 (19I8)_PCR/24 [Gene]SCO6306/21 NC_003888 SCIF3.08c, possible oxidoreductase, len: 376 aa; similar to TR:AAG05975 (EMBL:AE004687) Pseudomonas aeruginosa probable FAD-dependent monooxygenase PA2587, 382 aa; fasta scores: opt: 443 z-score: 500.7 E(): 2.4e-20; 31.4% identity in 354 aa overlap. Contains Pfam match to entry PF01494 FAD_binding_3, FAD binding domain and PF01360 Monooxygenase, Monooxygenase 2 3 7 9 [Reporter]SCO0021 (13F18)_PCR/4676 [Gene]SCO0021/4265 NC_003888 SCJ4.02, unknown, len: 197 aa 1 1 2 2 [Reporter]SCO1971 (20A8)_PCR/23 [Gene]SCO1971/20 NC_003888 SC3C9.06, conserved hypothetical protein, len: 301 aa; similar to TR:BAB36480 (EMBL:AP002560) Escherichia coli O157:H7 hypothetical 32.9 kDa protein ECS3057, 312 aa; fasta scores: opt: 837 Z-score: 923.0 bits: 178.9 E(): 8.4e-44; 48.495% identity in 299 aa overlap 2 3 7 8 [Reporter]SCO1474 (14B18)_PCR/4675 [Gene]SCO1474/4264 NC_003888 SCL6.31, unknown, len: 183 aa 2 3 7 7 [Reporter]SCO0811 (15N14)_PCR/4674 [Gene]SCO0811/4263 NC_003888 SCF43A.01c, probable ABC transporter ATP-binding protein, partial CDS, len: >104 aa; similar to many e.g. SW:ARAG_ECOLI (EMBL:X06091), araG, Escherichia coli L-arabinose transport ATP-binding protein (504 aa), fasta scores; opt: 305 z-score: 368.0 E(): 3.6e-13, 45.8% identity in 96 aa overlap. Similar to many others from S.coelicolor e.g. TR:O50504 (EMBL:AL009199) S.coelicolor probable ABC-transport system ATP binding protein (260 aa) (55.2% identity in 96 aa overlap). Contains Pfam match to PF00005 ABC_tran, ABC transporter and PS00017 ATP/GTP-binding site motif A (P-loop),SCF43.22c, possible ABC transporter, ATP-binding component, partial CDS, len: > 434 aa. Highly similar to many including: Escherichia coli SW:RBSA_ECOLI(EMBL:M13169) ribose transport ATP-binding protein RbsA (501 aa), fasta scores opt: 1301 z-score: 1408.5 E(): 0 45.7% identity in 431 aa overlap and Streptomyces coelicolor TR:O69942(EMBL:AL023862) ABC transporter ATP-binding protein SC3F9.02 (505 aa), fasta scores opt: 1179 z-score: 978.7 E():0 46.0% identity in 428 aa overlap. Contains 2x Pfam matches to entry PF00005 ABC_tran, ABC transporter and a Prosite hit to PS00211 ABC transporters family signature. 1 2 13 10 [Reporter]SCO3187 (13A3)_PCR/2289 [Gene]SCO3187/2089 NC_003888 SCE22.04, hypothetical protein, len: 110 aa; similar to TR:O05574 (EMBL:Z94752) Mycobacterium tuberculosis hypothetical 11.4 kD protein MTCI237.05c, 110 aa; fasta scores: opt: 379 z-score: 372.3 E(): 2.8e-13; 52.3% identity in 107 aa overlap. High content in serine amino acid residue in C-terminal domain 2 3 7 6 [Reporter]SCO3739 (16J14)_PCR/4673 [Gene]SCO3739/4262 NC_003888 SCH22A.17, possible epimerase, len: 334 aa; similar to TR:O69123 (EMBL:AF064070) Burkholderia pseudomallai putative epimerase/dehydratase WbiB, 363 aa; fasta scores: opt: 547 z-score: 590.5 E(): 1.8e-25; 35.9% identity in 351 aa overlap and to SW:GALE_ERWAM (EMBL:X76172) Erwinia amylovora UDP-glucose 4-epimerase (EC 5.1.3.2) GalE, 337 aa; fasta scores: opt: 241 z-score: 265.2 E(): 2.4e-07; 28.1% identity in 360 aa overlap. Contains Pfam match to entry PF01370 Epimerase, NAD dependent epimerase/dehydratase family 1 2 13 9 [Reporter]SCO3896 (13M23)_PCR/2288 [Gene]SCO3896/2088 NC_003888 SCH24.18, probable RNA nucleotidyltransferase, len: 483 aa; similar to tRNA nucleotidyltransferases e.g. SW:CCA_ECOLI (EMBL:M12788), cca, Escherichia coli tRNA nucleotidyltransferase (412 aa), fasta scores; opt: 413 z-score: 480.7 E(): 1.8e-19, 33.8% identity in 334 aa overlap. Also similar to mRNA poly(A) polymerases e.g. SW:PCNB_ECOLI (EMBL:S48039), pcnB, Escherichia coli poly(A) polymerase (472 aa) (32.0% identity in 256 aa overlap). Contains probable coiled-coil from 392 to 425 (34 residues) (Max score: 1.525, probability 0.97) 2 3 7 5 [Reporter]SCO3761 (17F14)_PCR/4672 [Gene]SCO3761/4261 NC_003888 SCH63.08c, unknown, len: 170 aa 1 1 19 12 [Reporter]SCO2602 (11A12)_PCR/407 [Gene]SCO2602/369 NC_003888 SCC88.13c, unknown, len: 707 aa 1 2 13 8 [Reporter]SCO0354 (14I23)_PCR/2287 [Gene]SCO0354/2087 NC_003888 SCF41.13, possible binding-protein-dependent transport protein, len: 280 aa, Similar to many e.g. Streptomyces coelicolor TR:O50501 (EMBL; AL009199) probable transmembrane transport protein SC7b7.04 (302 aa), fasta scores opt: 584 z-score: 670.3 E(): 5.5e-30 36.1% identity in 274 aa overlap. Contains multiple membrane spanning hydrophobic domains, a PS00402 Binding-protein-dependent transport systems inner membrane comp sign and a Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. 2 3 7 4 [Reporter]SCO1744 (18B14)_PCR/4671 [Gene]SCO1744/4260 NC_003888 SCI11.33, possible two-component system sensor kinase, len: 388 aa; similar to may putative sensor kinases e.g. to the N-terminus of TR:Q53893 (EMBL:AL035654), AbsA1, S.coelicolor putative two-component system sensor kinase involved in global regulation of antibiotic synthesis (571 aa), fasta scores; opt: 361 z-score: 391.6 E(): 1.8e-14, 31.6% identity in 364 aa overlap. The C-terminus is similar to part of SW:NARX_ECOLI (EMBL:X13360), NarX, NarR, Escherichia coli nitrate/nitrite sensor protein (598 aa) (30.7% identity in 218 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains probable coiled-coil from 157 to 189 (33 residues) (Max score: 1.493, probability 0.94) 1 1 19 11 [Reporter]SCO4638 (12M8)_PCR/406 [Gene]SCO4638/368 NC_003888 SCD82.09c, hypothetical protein, len: 274 aa; similar to TR:Q9XBC9 (EMBL:AL078635) Streptomyces coelicolor putative rRNA methylase CZA382.22c, 259 aa; fasta scores: opt: 259 z-score: 291.0 E(): 8.7e-09; 30.8% identity in 221 aa overlap 1 2 13 6 [Reporter]SCO1465 (16A23)_PCR/2285 [Gene]SCO1465/2086 NC_003888 SCL6.22c, possible secreted protein, len: 401 aa; similar to TR:Q55471 (EMBL:D64006) Synechocystis sp. hypothetical 46.5 kD protein, 421 aa; fasta scores: opt: 240 z-score: 260.1 E(): 4.6e-07; 28.8% identity in 385 aa overlap. Contains possible N-terminal region signal peptide sequence 1 1 19 10 [Reporter]SCO3093 (13I8)_PCR/405 [Gene]SCO3093/367 NC_003888 SCE41.02c, possible hydrolase, len: 313 aa; similar to N-terminal region of SW:GPPA_ECOLI (EMBL:M87049) Escherichia coli guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (EC 3.6.1.40) GppA, 494 aa; fasta scores: opt: 311 z-score: 357.2 E(): 2.2e-12; 28.4% identity in 310 aa overlap 1 2 13 5 [Reporter]SCO3813 (17M19)_PCR/2284 [Gene]SCO3813/2085 NC_003888 SCGD3.14, possible membrane protein, len: 328 aa; unknown function, probable CDS suggested by probable CDS suggested by positional base preference and GC frame analysis. Contains hydrophobic, possible membrane-spanning regions 1 1 19 9 [Reporter]SCO5161 (14E8)_PCR/404 [Gene]SCO5161/366 NC_003888 SCP8.24, hypothetical protein, len: 285 aa; similar to SW:TRPH_ECOLI (EMBL:AE000224) Escherichia coli TrpH protein, 293 aa; fasta scores: opt: 376 z-score: 429.3 E(): 1.9e-16; 30.6% identity in 248 aa overlap. Contains Pfam match to entry PF02231 PHP_N, PHP domain N-terminal region 1 2 13 4 [Reporter]SCP1.197 (18I19)_PCR/2283 [Gene]SCP1.197/2084 NC_003888 SCP1.197, unknown, len: 133aa; previously sequenced as TR:Q9RMI9 (EMBL:AF195093). 1 1 19 8 [Reporter]SCO0812 (15A8)_PCR/403 [Gene]SCO0812/365 NC_003888 SCF43A.02, possible sugar isomerase, len: 386 aa; weakly similar to many xylose isomerases (divergent at N- and C-termini) e.g. SW:XYLA_AMPSP (EMBL:M15050), XylA, Ampullariella sp. xylose isomerase (394 aa), fasta scores; opt: 189 z-score: 229.6 E(): 1.8e-05, 26.9% identity in 219 aa overlap 1 2 13 3 [Reporter]SCO4008 (19E19)_PCR/2282 [Gene]SCO4008/2083 NC_003888 2SC10A7.12, probable tetR family regulatory protein, len: 192 aa; similar to TR:CAB93030 (EMBL:AL357432) Streptomyces coelicolor putative transcriptional regulator SCD95A.03c, 199 aa; fasta scores: opt: 345 z-score: 436.9 E(): 7.9e-17; 35.8% identity in 187 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 1 1 19 7 [Reporter]SCO3648 (16M4)_PCR/402 [Gene]SCO3648/364 NC_003888 SCH10.26c, probable transmembrane efflux protein, len: 484 aa; similar to many members of the major facilitator family, e.g. SW:PUR8_STRLP (EMBL:X76855), pur8, Streptomyces lipmanii puromycin resistance protein (503 aa), fasta scores; opt: 871 z-score: 937.9 E(): 0, 37.3% identity in 475 aa overlap. Contains several hydrophobic, probable membrane-spanning regions. Similar to many putative efflux pumps from Streptomyces coelicolor e.g. TR:O69986 (EMBL:AL022268) SC4H2.31c, probable transmembrane efflux protein (515 aa) (38.8% identity in 412 aa overlap). Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter, score -78.80, E-value 0.003 1 2 13 2 [Reporter]SCO3984 (20M15)_PCR/2281 [Gene]SCO3984/2082 NC_003888 SCBAC25E3.21, conserved hypothetical protein, len: 67 aa: similar to TR:Q9X8D9 (EMBL:AL049573) hypothetical protein SCE39.16c from Streptomyces coelicolor (67 aa) fasta scores; opt: 257, Z-score: 349.2, 58.182% identity (58.182% ungapped) in 55 aa overlap. 1 1 19 6 [Reporter]SCO3832 (17I4)_PCR/401 [Gene]SCO3832/363 NC_003888 SCH69.02, possible transcriptional regulator, len: 135 aa; similar to many e.g. SW:LRP_ECOLI leucine-responsive regulatory protein from Escherichia coli (163 aa) fasta scores; opt: 181, z-score: 249.4, E(): 1.5e-06, (29.5% identity in 129 aa overlap). Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family and a probable helix-turn-helix 9-30 aa (+3.69 SD) 1 2 12 22 [Reporter]SCO1265 (1M11)_PCR/2279 [Gene]SCO1265/2081 NC_003888 2SCG18.12c, probable lipase, len: 289 aa; similar to TR:Q59644 (EMBL:X99255) Propionibacterium acnes triacylglycerol lipase precursor (EC 3.1.1.3) GehA, 339 aa; fasta scores: opt: 423 z-score: 498.8 E(): 2.7e-20; 36.9% identity in 233 aa overlap and to TR:Q9S295 (EMBL:AL096849) Streptomyces coelicolor putative secreted lipase SCI11.24c, 290 aa; fasta scores: opt: 682 z-score: 722.5 E(): 1.2e-34; 47.0% identity in 264 aa overlap. Contains Pfam match to entry PF01674 Lipase_2, Lipase 1 1 19 4 [Reporter]SCO4982 (19A4)_PCR/399 [Gene]SCO4982/362 NC_003888 2SCK36.05, putative membrane protein, len: 60 aa. Contains possible hydrophobic membrane spanning region 1 2 12 21 [Reporter]SCO5694 (2I11)_PCR/2278 [Gene]SCO5694/2080 NC_003888 SC5H4.18, dxr, 1-deoxy-D-xylulose 5-phosphate reductoisomerase, len: 418 aa; highly similar to SW:DXR_MYCTU (EMBL:Z74024) Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase (EC 1.1.1.-) (DXP reductoisomerase) Dxr or MTCY274.01c, 436 aa; fasta scores: opt: 1079 z-score: 1175.5 E(): 0; 52.3% identity in 396 aa overlap and to TR:CAB60758 (EMBL:AJ250714) Zymomonas mobilis 1-deoxy-D-xylulose 5-phosphate reductoisomerase Dxr, 388 aa; fasta scores: opt: 966 z-score: 1053.7 E(): 0; 44.0% identity in 389 aa overlap 1 1 19 3 [Reporter]SCO1864 (19M24)_PCR/398 [Gene]SCO1864/361 NC_003888 SCI39.11, probable acetyltransferase, len: 170 aa; similar to SW:ECTA_MARHA (EMBL:U66614) Marinococcus halophilus L-2,4-diaminobutyric acid acetyltransferase (EC 2.3.1.*) EctA, 172 aa; fasta scores: opt: 443 Z-score: 540.9 bits: 106.5 E(): 1.5e-22; 42.675% identity in 157 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 1 19 2 [Reporter]SCO3994 (20E24)_PCR/397 [Gene]SCO3994/360 NC_003888 SCBAC25E3.31, unknown, doubtful CDS, len: 70aa: no significant database matches. 3 4 1 9 [Reporter]SCO3109 (13O9)_PCR/7063 [Gene]SCO3109/6439 NC_003888 SCE41.18c, probable transcriptional-repair coupling factor, len: 1184 aa; similar to SW:MFD_ECOLI (EMBL:AE000211) Escherichia coli transcriptional-repair coupling factor Mfd, 1148 aa; fasta scores: opt: 2043 z-score: 2247.7 E(): 0; 37.5% identity in 1181 aa overlap . Contains Pfam match to entry PF00270 DEAD, DEAD/DEAH box helicase and PF00271 helicase_C, Helicases conserved C-terminal domain and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 3 4 1 8 [Reporter]SCO1798 (14K9)_PCR/7062 [Gene]SCO1798/6438 NC_003888 SCI5.06c, possible ABC transporter ATP-binding subunit, len: 265aa; similar to many egs. TR:O53288 (EMBL:AL021287) predicted ABC transporter ATP-binding subunit (287 aa) fasta scores; opt: 893, z-score: 998.3, E(): 0, (53.3% identity in 257 aa overlap) and SW:LIVG_SALTY high-affinity branched-chain amino acid transporter from Salmonella typhimurium (255 aa) fasta scores; opt: 343, z-score: 390.2, E(): 2.1e-14, (31.0% identity in 248 aa overlap). Contains Pfam match to PF00005 ABC transporter transmembrane region and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 3 4 1 7 [Reporter]SCO4952 (15G9)_PCR/7061 [Gene]SCO4952/6437 NC_003888 2SCK31.12, probable tetR-family transcriptional regulator, len: 198 aa; similar to TR:Q9RJL5 (EMBL:AL121849) Streptomyces coelicolor putative tetR-family transcriptional regulator SCF6.16, 194 aa; fasta scores: opt: 355 z-score: 451.5 E(): 1.3e-17; 35.1% identity in 185 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 42..63 (+4.18 SD) 3 4 1 6 [Reporter]SCO0148 (16C9)_PCR/7060 [Gene]SCO0148/6436 NC_003888 SCJ33.12, possible transcriptional regulatory protein, len: 81 aa. Highly similar to Bacillus subtilis TR:O31834 (EMBL:Z99114) YozG protein (84 aa), fasta scores opt: 307 z-score: 443.1 E(): 2.6e-17 61.8% identity in 68 aa overlap. Contains a Pfam match to entry PF01381 HTH_3, Helix-turn-helix which lies between residues 17..38 (+3.45 SD). 1 1 1 22 [Reporter]SCO5201 (1A4)_PCR/21 [Gene]SCO5201/19 NC_003888 2SC3B6.25, moaE-like protein, len: 152 aa; similar to SW:MOAE_ECOLI (EMBL:X70420) Escherichia coli molybdopterin [mpt] converting factor, subunit 2 MoaE, 149 aa; fasta scores: opt: 255 z-score: 317.2 E(): 3.3e-10; 33.3% identity in 129 aa overlap 3 4 1 5 [Reporter]SCO0664 (17O5)_PCR/7059 [Gene]SCO0664/6435 NC_003888 SCF91.24, hypothetical protein, len: 631 aa; similar to TR:CAC48915 (EMBL:AL603643) Rhizobium meliloti (Sinorhizobium meliloti) hypothetical protein SMB20536, 617 aa; fasta scores: opt: 524 Z-score: 553.7 E(): 3.3e-23; 30.182% identity in 550 aa overlap 1 1 1 21 [Reporter]SCO4919 (1M24)_PCR/20 [Gene]SCO4919/18 NC_003888 SCK13.11, probable oxidoreductase, len: 475 aa; similar to SW:DLD2_BACSU (EMBL:D84432) Bacillus subtilis dihydrolipoamide dehydrogenase (EC 1.8.1.4) BfmBC, 474 aa; fasta scores: opt: 753 z-score: 813.4 E(): 0; 29.9% identity in 472 aa overlap. Contains Pfam match to entry PF00070 pyr_redox, Pyridine nucleotide-disulphide oxidoreductase 3 4 1 4 [Reporter]SCO0589 (18K5)_PCR/7058 [Gene]SCO0589/6434 NC_003888 SCF55.13c, probable lysR-family transcriptional regulator, len: 295 aa; similar to SW:GLTR_BACSU (EMBL:U79494) Bacillus subtilis transcriptional regulatory protein GltR, 296 aa; fasta scores: opt: 421 z-score: 502.5 E(): 1.3e-20; 33.6% identity in 244 aa overlap. Contains match to Pfam entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Contains possible helix-turn-helix motif at residues 16..37 (+4.59 SD) 1 1 1 20 [Reporter]SCO4566 (2I24)_PCR/19 [Gene]SCO4566/17 NC_003888 SCD16A.17c, nuoE, NADH dehydrogenase subunit, len: 290 aa; similar to many e.g. TR:P95177 (EMBL:Z83867) NuoE, NADH dehydrogenase subunit from Mycobacterium tuberculosis (252 aa) fasta scores; opt: 1002, z-score: 1072.0, E(): 0, (69.5% identity in 213 aa overlap) and SW:NUHM_RAT NADH-ubiquinone dehydrogenase subunit from Rattus norvegicus (Rat) (241 aa) fasta scores; opt: 419, z-score: 454.9, E(): 5.2e-18, (38.1% identity in 181 aa overlap). Contains Pfam match to entry PF01257 complex1_24kD, Respiratory-chain NADH dehydrogenase 24 Kd subunit. 3 4 1 3 [Reporter]SCO4898 (19G5)_PCR/7057 [Gene]SCO4898/6433 NC_003888 2SCK8.24c, probable tetR-family transcriptional regulator, len: 193 aa; similar to TR:Q9Z551 (EMBL:AL035212) Streptomyces coelicolor putative transcriptional regulator SC9B2.08c, 192 aa; fasta scores: opt: 235 z-score: 290.5 E(): 1.3e-08; 35.3% identity in 187 aa overlap and to SW:BETI_ECOLI (EMBL:X52905) Escherichia coli regulatory protein BetI, 195 aa; fasta scores: opt: 161 z-score: 202.7 E(): 0.001; 28.5% identity in 186 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 32..53 (+4.35 SD) 1 1 1 19 [Reporter]SCO6332 (3E24)_PCR/18 [Gene]SCO6332/16 NC_003888 SC10H5.08c, unknown, len: 65 aa 2 4 23 22 [Reporter]SCO1752 (1J21)_PCR/7055 [Gene]SCO1752/6432 NC_003888 2SCI34.05, possible integral membrane protein, len: 396 aa; similar to TR:Q9L0K6 (EMBL:AL161691) Streptomyces coelicolor putative integral membrane protein SCD40A.03c, 434 aa; fasta scores: opt: 678 Z-score: 715.1 E(): 3.4e-32; 37.530% identity in 413 aa overlap. Contains possible hydrophobic membrane spanning regions 1 1 1 18 [Reporter]SCO6199 (4A24)_PCR/17 [Gene]SCO6199/15 NC_003888 SC2G5.20, possible secreted esterase, len: 743aa; similar to TR:Q54345 (EMBL:Z50108) esterase, FusH (509 aa) from Streptomyces lividans fasta scores; opt: 619, z-score: 601.5, E(): 3.2e-26,(40.9% identity in 389 aa overlap). Note that the CDS contains Pfam match to entry PF00089 trypsin,Trypsin, score 1.40, E-value 5.6e-05. Also contains possible N-terminal signal sequence. 2 3 7 3 [Reporter]SCO4040 (19N10)_PCR/4670 [Gene]SCO4040/4259 NC_003888 2SCD60.06, unknown, len: 154 aa 2 4 23 21 [Reporter]SCO3873 (2F21)_PCR/7054 [Gene]SCO3873/6431 NC_003888 SCH18.10c, gyrA, DNA gyrase subunit A, len: 864 aa; identical to previously sequenced SW:GYRA_STRCO (EMBL:L27063) Streptomyces coelicolor DNA gyrase subunit A (EC 5.99.1.3) GryA, 864 aa and similar to TR:O50510 (EMBL:AL009204) S. coelicolor SC9B10.03c, 818 aa; fasta scores: opt: 1898 z-score: 2031.4 E(): 0; 43.1% identity in 788 aa overlap. Contains Pfam match to entry PF00521 DNA_topoisoIV, DNA gyrase/topoisomerase IV, subunit A 1 1 1 17 [Reporter]SCO7044 (5M20)_PCR/16 [Gene]SCO7044/14 NC_003888 SC4G1.10, possible integral membrane protein, len: 154 aa; similar to TR:P95089 (EMBL:Z83866) Mycobacterium tuberculosis hypothetical 14.3 kDa protein MTCY22D7.12c, 132 aa; fasta scores: opt: 258 z-score: 319.8 E(): 2.4e-10; 42.1% identity in 121 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 7 2 [Reporter]SCO1662 (20F10)_PCR/4669 [Gene]SCO1662/4258 NC_003888 SCI52.04, conserved hypothetical protein, len: 333 aa; similar to TR:O33260 (EMBL:Z97559) Mycobacterium tuberculosis hypothetical 31.8 kDa protein MTCY261.21, 292 aa; fasta scores: opt: 1072 Z-score: 902.2 bits: 175.1 E(): 1.1e-42; 57.554% identity in 278 aa overlap. Contains 3x degenerate repeat unit: PGG 2 4 23 20 [Reporter]SCO7380 (3B21)_PCR/7053 [Gene]SCO7380/6430 NC_003888 SC10G8.07c, unknown, len: 437 aa. Similar in parts to several proteins of undefined function e.g. Streptomyces coelicolor TR:Q9ZBJ0 (EMBL:AL035161) conserved hypothetical protein, SC9C7.20 (860 aa), fasta scores opt: 271 z-score: 309.7 E(): 7.7e-10 30.3% identity in 274 aa overlap 1 1 1 16 [Reporter]SCO7303 (6I20)_PCR/15 [Gene]SCO7303/13 NC_003888 SC5F8.13, possible TetR-family regulator, len: 193 aa. Weakly similar to Pseudomonas putida TR:O33453(EMBL:U24215) regulator of the p-cymene and p-Cumate catabolic operons, CymR (203 aa), fasta scores opt: 174 z-score: 221.1 E(): 7.6e-05 28.2% identity in 174 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. 2 3 6 22 [Reporter]SCO5390 (1F6)_PCR/4667 [Gene]SCO5390/4257 NC_003888 2SC6G5.34c, possible alkanal monooxygenase (luciferase), len: 342aa; similar to luciferase alpha chain from many eg. SW:P19839 (LXA1_PHOLU) alkanal monooxygenase alpha chain (EC 1.14.14.3) (bacterial luciferase alpha chain) from Photorhabdus luminescens (Xenorhabdus luminescens) (360 aa) fasta scores; opt: 442, z-score: 520.4, E(): 1.6e-21, 30.1% identity in 359 aa overlap. Contains Pfam match to entry PF00296 bac_luciferase, Bacterial luciferase. 1 1 1 15 [Reporter]SCO7412 (7E20)_PCR/14 [Gene]SCO7412/12 NC_003888 SC6D11.08, possible pyruvate dehydrogenase (pyruvate oxidase), len: 600 aa. Highly similar to many including: Escherichia coli SW:POXB_ECOLI(EMBL:X04105) pyruvate dehydrogenase (572 aa), fasta scores opt: 770 z-score: 855.7 E(): 0 34.6% identity in 566 aa overlap and Streptomyces coelicolor TR:Q9ZBT3(EMBL:AL034446) pyruvate dehydrogenase (pyruvate oxidase), SC1A9.19 (580 aa), fasta scores opt: 866 z-score: 962.2 E():0 33.1% identity in 556 aa overlap. Contains a Pfam match to entry PF00205 TPP_enzymes, Thiamine pyrophosphate enzymes. Also contains a Prosite hit to PS00187 Thiamine pyrophosphate enzymes signature. 2 3 6 21 [Reporter]SCO0789 (2B6)_PCR/4666 [Gene]SCO0789/4256 NC_003888 3SCF60.21, conserved hypothetical protein, len: 372 aa; C-terminal region identical to TR:P72464 (EMBL:Z70724) Streptomyces lividans hypothetical protein ORF1, 343 aa and similar to TR:P96221 (EMBL:Z83864) Mycobacterium tuberculosis hypothetical 36.7 kDa protein MTCY01A6.12, 335 aa; fasta scores: opt: 1286 z-score: 1468.5 E(): 0; 61.0% identity in 336 aa overlap. Contains Pfam match to entry PF02231 PHP_N, PHP domain N-terminal region 1 1 1 14 [Reporter]SCO6877 (8A20)_PCR/13 [Gene]SCO6877/11 NC_003888 SC7F9.29c, unknown, len: 260 aa. 2 3 6 20 [Reporter]SCO1464 (3N2)_PCR/4665 [Gene]SCO1464/4255 NC_003888 SCL6.21c, rpe, ribulose-phosphate 3-epimerase, len: 228 aa; highly similar to SW:RPE_BACSU (EMBL:Y13937) Bacillus subtilis ribulose-phosphate 3-epimerase (EC 5.1.3.1) Rpe, 217 aa; fasta scores: opt: 659 z-score: 776.1 E(): 0; 48.1% identity in 212 aa overlap. Contains Pfam match to entry PF00834 Ribul_P_3_epim, Ribulose-phosphate 3 epimerase family and matches to Prosite entries PS01085 Ribulose-phosphate 3-epimerase family signature 1 and PS01086 Ribulose-phosphate 3-epimerase family signature 2 1 1 1 13 [Reporter]SCO6125 (9M16)_PCR/12 [Gene]SCO6125/10 NC_003888 SC9B2.12, possible membrane lipoprotein, len: 219aa; Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 2 3 6 19 [Reporter]SCO6041 (4J2)_PCR/4664 [Gene]SCO6041/4254 NC_003888 SC1B5.01, possible hemY, protoporphyrinogen oxidase, partial CDS, len: > 468 aa; similar to many e.g. HEMG_BACSU P32397 bacillus subtilis. protoporphyrinogen oxidase (470 aa), fasta scores; opt: 741 z-score: 711.8 E(): 2e-32, 32.9% identity in 453 aa overlap,SC1C3.29, possible hemY, protoporphyrinogen oxidase, partial CDS, len: > 61 aa; overlaps and ecxtends CDS from overlapping cosmid, SC1B5.01 2 3 6 18 [Reporter]SCO6341 (5F2)_PCR/4663 [Gene]SCO6341/4253 NC_003888 SC3A7.09, probable exonuclease, len: 259 aa; similar to exonuclease III from many organisms e.g. EX3_ECOLI exodeoxyribonuclease III (EC 3.1.11.2) (268 aa), fasta scores; opt: 302 z-score: 443.2 E(): 2e-17, 33.5% identity in 266 aa overlap 1 2 12 20 [Reporter]SCO1481 (3E11)_PCR/2277 [Gene]SCO1481/2079 NC_003888 SC9C5.05c, pyrF, orotidine 5'-phosphate decarboxylase, len: 278 aa; similar to SW:DCOP_MYCTU (EMBL:Z80108) Mycobacterium tuberculosis orotidine 5'-phosphate decarboxylase (EC 4.1.1.23) PyrF or UraA, 274 aa; fasta scores: opt: 800 z-score: 874.6 E(): 0; 51.1% identity in 282 aa overlap. Contains Pfam match to entry PF00215 OMPdecase, Orotidine 5'-phosphate decarboxylases and match to Prosite entry PS00156 Orotidine 5'-phosphate decarboxylase active site 2 3 6 17 [Reporter]SCO7345 (6B2)_PCR/4662 [Gene]SCO7345/4252 NC_003888 SC4G10.24c, probable ATP-dependent DNA ligase, len: 326 aa. Similar in parts to many DNA ligases e.g. Pyrococcus horikoshii SW:DNLI_PYRHO(EMBL:AP000006) probable thermostable DNA ligase (EC 6.5.1.1) (559 aa), fasta scores opt: 454 z-score: 506.0 E(): 1e-20 32.0% identity in 344 aa overlap. Contains a Prosite hit to PS00697 ATP-dependent DNA ligase AMP-binding site and a Pfam match to entry PF01068 DNA_ligase, DNA ligase. 1 2 12 19 [Reporter]SCO5557 (4A11)_PCR/2276 [Gene]SCO5557/2078 NC_003888 SC1C2.38c, unknown, len: 77 aa 2 3 6 16 [Reporter]SCO4838 (6N22)_PCR/4661 [Gene]SCO4838/4251 NC_003888 SC5G8.06c, unknown, len: 218aa; 1 1 18 22 [Reporter]SCO1131 (1E20)_PCR/395 [Gene]SCO1131/359 NC_003888 2SCG38.24c, conserved hypothetical protein, len: 385 aa; similar to TR:O53711 (EMBL:AL021931) Mycobacterium tuberculosis hypothetical 39.9 kDa protein MTV036.11c, 380 aa; fasta scores: opt: 1176 z-score: 1285.6 E(): 0; 56.7% identity in 351 aa overlap and to TR:Q9ZBN5 (EMBL:AL034492) Streptomyces coelicolor hypothetical 40.7 kDa protein SC6C5.10, 395 aa; fasta scores: opt: 1764 z-score: 1925.1 E(): 0; 76.9% identity in 351 aa overlap 1 2 12 18 [Reporter]SCO2069 (5M7)_PCR/2275 [Gene]SCO2069/2077 NC_003888 SC4A10.02c, hypothetical protein, len: 196 aa; similar to the plasmid borne TR:CAC48425 (EMBL:AL603642) Rhizobium meliloti (Sinorhizobium meliloti) conserved hypothetical protein SMB20022, 188 aa; fasta scores: opt: 485 Z-score: 576.0 E(): 1.9e-24; 45.198% identity in 177 aa overlap. Probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Annotated in cosmid 4G6 as SC4G6.38c 2 3 6 15 [Reporter]SCO6961 (7J22)_PCR/4660 [Gene]SCO6961/4250 NC_003888 SC6F7.14c, unknown, len: 382 aa. Similar to several proteins of undefined function e.g. Streptomyces coelicolor TR:Q9XAC4 (EMBL:AL096839) SCC22.14C (368 aa), fasta scores opt: 945 z-score: 1101.8 E():0 47.7% identity in 369 aa overlap. 1 1 18 21 [Reporter]SCO0651 (2A20)_PCR/394 [Gene]SCO0651/358 NC_003888 SCF91.11, gvpF2, possible gas vesicle synthesis protein, len: 261 aa. Highly similar to many other gas vesicle synthesis proteins including: Bacillus megaterium TR:O68674(EMBL:AF053765) gas vesicle protein GvpF (255 aa), fasta scores opt: 181 z-score: 209.5 E(): 0.00027 24.2% identity in 211 aa overlap and Streptomyces coelicolor TR:Q9ZC12 (EMBL:AL033505) putative gas vesicle synthesis protein GvpF (257 aa), fasta scores opt: 459 z-score: 515.7 E(): 2.4e-21 38.9% identity in 244 aa overlap. 1 2 12 17 [Reporter]SCO5828 (6I7)_PCR/2274 [Gene]SCO5828/2076 NC_003888 SC5B8.18c, probable two-component transcriptional regulator, len: 226 aa; similar to many e.g. CITB_KLEPN P5268 8 transcriptional regulator from klebsiella pneumoniae (23 4 aa), fasta scores; opt: 319 z-score: 394.9 E(): 8.3e-15, 30.8% identity in 224 aa overlap. Contains Pfam match to entry response_reg PF00072, Response regulator receiver domain, score 117.43 and probable helix-turn-helix from aa 176- 197 (Score 2073, +6.25 SD) 1 1 18 20 [Reporter]SCO7694 (3M16)_PCR/393 [Gene]SCO7694/357 NC_003888 SC1A4.02, probable tetR-family transcriptional regulator, len: 190 aa; similar to TR:Q9PBQ5 (EMBL:AE004024) Xylella fastidiosa transcriptional regulator (AcrR family) XF2085, 205 aa; fasta scores: opt: 170 z-score: 215.3 E(): 0.00019; 30.8% identity in 117 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 38..59 (+4.46 SD) 1 2 12 16 [Reporter]SCO7265 (7E7)_PCR/2273 [Gene]SCO7265/2075 NC_003888 SC5H1.27, hypothetical protein, len: 199 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 1 1 18 19 [Reporter]SCO7222 (4I16)_PCR/392 [Gene]SCO7222/356 NC_003888 SC2H12.21c, possible TetR-family transcriptional regulator, len: 236 aa. Similar to several including: Escherichia coli SW:TER1_ECOLI (EMBL:X61367) tetracycline repressor protein class A (216 aa), fasta scores opt: 183 z-score: 217.2 E(): 0.00013 28.2% identity in 209 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 36..57 (+4.19 SD). 1 2 12 15 [Reporter]SCO4757 (8A7)_PCR/2272 [Gene]SCO4757/2074 NC_003888 SC6G4.35, unknown, len: 419 aa; similar to hypotheti cal protein from Rhizobium meliloti TR:Q52997 (EMBL:L39265) (66 aa, partial) opt: 154 z-score: 162.6 E(): 0.086, 40.0% identity in 50 aa overlap. N-terminus is weakly similar to several sigma factors e.g. CARQ_MYXXA RNA polymerase sigma factor carQ (174 aa), fasta scores; opt: 153 z-score: 172. 4 E(): 0.024, 29.8% identity in 161 aa overlap 1 1 18 18 [Reporter]SCO2399 (5E16)_PCR/391 [Gene]SCO2399/355 NC_003888 SC4A7.27, hypothetical protein, len: 293 aa; low similarity to TR:Q9XAH5 (EMBL:AL096825) Streptomyces coelicolor hypothetical 60.3 kD protein SC6G3.05, 588 aa; fasta scores: opt: 128 z-score: 149.7 E(): 0.59; 28.8% identity in 313 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 2 12 14 [Reporter]SCO7808 (9M3)_PCR/2271 [Gene]SCO7808/2073 NC_003888 SC8E7.05c, unknown, len: 97 aa. Highly similar to the N-terminal of Streptomyces coelicolor mini-circle putative transposase for IS11 SW:YM3_STRCO (EMBL:X15942) SC3C8.19 (414 aa), fasta scores opt: 239 z-score: 303.4 E(): 2e-09 44.6% identity in 92 aa overlap. 1 1 18 17 [Reporter]SCO7776 (6A16)_PCR/390 [Gene]SCO7776/354 NC_003888 SC5E9.24, unknown, len: 106 aa; some similarity to TR:Q9Z3X0 (EMBL:AF073359) 4-oxalocrotonate isomerase from Sphingomonas sp. DJ77 (77 aa) fasta scores; opt: 91, z-score: 135.8, E(): 4.3, 32.8% identity in 61 aa overlap. 1 2 12 13 [Reporter]SCO6263 (10I3)_PCR/2270 [Gene]SCO6263/2072 NC_003888 SCAH10.28, conserved hypothetical protein, len: 797 aa; C-terminus identical to previously sequenced TR: O86854 (EMBL:AJ007731) Streptomyces coelicolor hypothetical 59.8 KD protein orfZ, 566 aa; fasta scores: opt: 3713 z-score: 2921.3 E(): 0; 96.6% identity in 559 aa overlap. Regions similar to others eg. TR:AAK46444 (EMBL:AE007065). 1 1 18 16 [Reporter]SCO5652 (7M12)_PCR/389 [Gene]SCO5652/353 NC_003888 SC6A9.15, conserved hypothetical protein, len: 135 aa; similar to TR:Q98D71 (EMBL:AP003005) hypothetical protein from Rhizobium loti (134 aa) fasta scores; opt: 401, Z-score: 482.3, E(): 3.1e-19, 46.212% identity (46.565% ungapped) in 132 aa overlap. 1 2 12 12 [Reporter]SCO2262 (11E3)_PCR/2269 [Gene]SCO2262/2071 NC_003888 SCC75A.08c, possible oxidoreductase, len: 239 aa. Similar to several including: Methanobacterium thermoautotrophicum TR:O59661(EMBL:Y17210) F420-dependent NADP reductase (224 aa), fasta scores opt: 257 z-score: 283.4 E(): 2.1e-08 38.3% identity in 222 aa overlap and Archaeoglobus fulgidus TR:O29059(EMBL:AE001021) conserved hypothetical protein (213 aa), fasta scores opt: 397 z-score: 431.0 E(): 1.2e-16 37.3% identity in 204 aa overlap. 1 1 18 15 [Reporter]SCO5754 (8I12)_PCR/388 [Gene]SCO5754/352 NC_003888 SC7C7.09, unknown, len: 181 aa; similar to competence damage induced proteins of B. subtilis and Streptococcus pneumoniae e.g. CINA_STRPN putative competence-damage protein (418 aa), fasta scores; opt: 304 z-score: 431.6 E(): 8.4e-17, 35.8% identity in 165 aa overlap. Contains probable coiled-coil from 133 to 175 1 2 12 11 [Reporter]SCO4773 (12A3)_PCR/2268 [Gene]SCO4773/2070 NC_003888 SCD63.05c, probable nucleotide-sugar dehydrogenase, len: 405 aa; similar to SW:WECC_ECOLI (EMBL:M87049) Escherichia coli UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (EC 1.1.1.-) WecC, 420 aa; fasta scores: opt: 565 z-score: 606.3 E(): 2.4e-26; 31.7% identity in 420 aa overlap. Contains Pfam match to entry PF00984 UDPG_MGDP_dh, UDP-glucose/GDP-mannose dehydrogenase family 1 1 18 14 [Reporter]SCO2634 (9E12)_PCR/387 [Gene]SCO2634/351 NC_003888 SC8E4A.04c, hypothetical protein, len: 216 aa; similar to various hypothetical proteins, e.g. TR:O53193 (EMBL:AL021246) Mycobacterium tuberculosis hypothetical 23.0 kD protein MTV008.22c, 207 aa; fasta scores: opt: 789 z-score: 898.4 E(): 0; 54.9% identity in 213 aa overlap 1 1 18 13 [Reporter]SCO6238 (10A12)_PCR/386 [Gene]SCO6238/350 NC_003888 SCAH10.03, hypothetical protein, len: 221 aa; similar to various hypothetical proteins, e.g. TR:O53480 (EMBL:AL021899) Mycobacterium tuberculosis hypothetical 23.2 kD protein, 213 aa; fasta scores: opt: 381 z-score: 468.2 E(): 1e-18; 38.3% identity in 201 aa overlap 2 4 23 19 [Reporter]SCO7604 (4N17)_PCR/7052 [Gene]SCO7604/6429 NC_003888 SC2H2.02, hypothetical protein, len: 294 aa; similar to TR:Q9S1Z5 (EMBL:AL109747) Streptomyces coelicolor putative secreted protein SCJ21.12c, 279 aa; fasta scores: opt: 321 z-score: 379.2 E(): 1.4e-13; 33.1% identity in 278 aa overlap 2 4 23 18 [Reporter]SCO6696 (5J17)_PCR/7051 [Gene]SCO6696/6428 NC_003888 SC4C6.06, possible regulatory protein, len: 893 aa; similar to many putative regulators e.g. TR:Q55103 (EMBL:D13457) Streptomyces sp. ORF upstream of the cholesterol oxidase-cytochrome P450 operon (642 aa) fasta scores; opt: 347 z-score: 383.0 E(): 5.2e-14, 31.7% identity in 599 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Similar to (EMBL:AL035569) SC8D9.18 possible regulatory protein (1091 aa) (28.1% identity in 945 aa overlap) 2 4 23 17 [Reporter]SCO7548 (6F17)_PCR/7050 [Gene]SCO7548/6427 NC_003888 SC5F1.02, conserved hypothetical protein, len: 314 aa; similar to TR:P95060 (EMBL:Z84395) Mycobacterium tuberculosis hypothetical 32.7 kDa protein MTCY210.31, 299 aa; fasta scores: opt: 704 z-score: 772.7 E(): 0; 47.2% identity in 301 aa overlap 2 4 23 16 [Reporter]SCO2661 (7B17)_PCR/7049 [Gene]SCO2661/6426 NC_003888 SC6D10.04, possible sugar hydrolase, len: 421 aa; similar to SW:CELF_ECOLI (EMBL:X52890) Escherichia coli 6-phospho-beta-glucosidase (EC 3.2.1.86) CelF, 450 aa; fasta scores: opt: 556 z-score: 624.0 E(): 2.4e-27; 36.0% identity in 431 aa overlap and to SW:AGAL_ECOLI (EMBL:X04894) Escherichia coli alpha-galactosidase (EC 3.2.1.22) (melibiase) MelA, 451 aa; fasta scores: opt: 300 z-score: 338.4 E(): 1.9e-11; 25.5% identity in 459 aa overlap 2 4 23 15 [Reporter]SCO5965 (8N13)_PCR/7048 [Gene]SCO5965/6425 NC_003888 SC7H1.35, possible integral membrane protein, len: 155 aa; weakly similar to hypothetival proteins from Archaeoglobus fulgidus and Mycobacterium leprae e.g. TR:G2650576 (EMBL:AE001101) AF0081 hypothetical 17.4 kd protein from Archaeoglobus fulgidus (156 aa), fasta scores; opt: 129 z-score: 186.3 E(): 0.0035, 28.7% identity in 101 aa overlap and similar to TR:O86494 (EMBL:AL031232) Streptomyces coelicolor putative integral membrane protein SC10H5.02c, 151 aa; fasta scores: opt: 431 Z-score: 491.5 E(): 9.6e-20; 53.509% identity in 114 aa overlap. Contains possible hydrophobic membrane spannig regions 2 4 23 14 [Reporter]SCO5838 (9J13)_PCR/7047 [Gene]SCO5838/6424 NC_003888 SC9B10.05, possible protease, len: 462; weakly similar to several hypothetical proteases eg. TR:P73669 (EMBL:D90908) processing protease from Synechocystis sp. (435 aa), fasta scores; opt: 363 z-score: 293.0 E(): 3.7e-09, 26.0% identity in 439 aa overlap 2 4 23 13 [Reporter]SCO1927 (10F13)_PCR/7046 [Gene]SCO1927/6423 NC_003888 SCC22.09, possible aminoglycoside acetyltransferase, len: 262 aa; similar to many e.g. SW:AAC8_STRFR aminoglycoside acetyltransferase from Streptomyces fradiae (286 aa) fasta scores; opt: 502, z-score: 576.5, E(): 8.9e-25, (40.4% identity in 270 aa overlap) 2 4 23 12 [Reporter]SCO4555 (11B13)_PCR/7045 [Gene]SCO4555/6422 NC_003888 SCD16A.28c, possible integral membrane transferase, len: 363aa; similar to TR:Q56803 (EMBL:X78451) O-acetyl transferase from Xanthomonas campestris (364 aa) fasta scores; opt: 123, z-score: 149.6, E(): 0.53, (26.2% identity in 378 aa overlap). Contains possible membrane spanning hydrophobic regions. 2 3 6 14 [Reporter]SCO5935 (8F22)_PCR/4659 [Gene]SCO5935/4249 NC_003888 SC7H1.05, putative membrane protein, len: 297 aa. Contains possible hydrophobic membrane spanning region 2 4 23 11 [Reporter]SCO4143 (12N9)_PCR/7044 [Gene]SCO4143/6421 NC_003888 SCD84.10c, possible mutT-like protein, len: 142 aa; similar to TR:O93721 (EMBL:U82368) Pyrobaculum aerophilum diadenosine 5'5'''-P1,P4-tetraphosphate pyrophosphohydrolase, 143 aa; fasta scores: opt: 169 z-score: 214.0 E(): 0.00018; 37.5% identity in 120 aa overlap, to TR:P96590 (EMBL:AB001488) Bacillus subtilis MutT protein, 149 aa; fasta scores: opt: 148 z-score: 189.0 E(): 0.0046; 28.2% identity in 110 aa overlap and to SW:MUTX_STRPN (EMBL:Z21702) Streptococcus pneumoniae mutator MutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (8-oxo-DGTPAse) (EC 3.6.1.-) MutX, 154 aa; blastp scores: Score = 87 (30.6 bits), Expect = 0.0081, P = 0.0080 Identities = 35/117 (29%), Positives = 54/117 (46%). Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein and match to Prosite entry PS00893 mutT domain signature 2 3 6 13 [Reporter]SCO6056 (9B22)_PCR/4658 [Gene]SCO6056/4248 NC_003888 SC9B1.03c, possible secreted protein, len: 133aa; contains possible N-terminal signal sequence. 2 4 23 10 [Reporter]SCO3104 (13J9)_PCR/7043 [Gene]SCO3104/6420 NC_003888 SCE41.13c, unknown, len: 679 aa 2 3 6 12 [Reporter]SCO1962 (10N18)_PCR/4657 [Gene]SCO1962/4247 NC_003888 SCC54.22c, probable ionic transporter, len: 366 aa; similar to many egs. TR:O53910 (EMBL:AL022001) putative ionic transporter from Mycobacterium tuberculosis (360 aa) fasta scores; opt: 1466, z-score: 1650.6, E(): 0, (69.4% identity in 356 aa overlap) and SW:CHAA_ECOLI ChaA, calcium/proton antiporter from Escherichia coli (366 aa) fasta scores; opt: 668, z-score: 756.2, E(): 0, (34.7% identity in 352 aa overlap). Contains several probable membrane spanning hydrophobic regions. 2 3 6 11 [Reporter]SCO4530 (11J18)_PCR/4656 [Gene]SCO4530/4246 NC_003888 2SCD4.01, possible membrane protein (fragment), len: >147 aa. Contains possible hydrophobic membrane spanning region,SCD35.37, unknown, len: >77 aa 2 3 6 10 [Reporter]SCO4288 (12F18)_PCR/4655 [Gene]SCO4288/4245 NC_003888 SCD95A.21, possible phosphatase, len: 290 aa; similar to TR:O27788 (EMBL:AE000931) Methanobacterium thermoautotrophicum trehalose-6-phosphate phophatase related protein MTH1760, 264 aa; fasta scores: opt: 197 z-score: 223.2 E(): 5.7e-05; 28.3% identity in 258 aa overlap 2 3 6 9 [Reporter]SCO3879 (13B18)_PCR/4654 [Gene]SCO3879/4244 NC_003888 SCH18.16c, dnaA, chromosomal replication initiator protein (fragment), len: >606 aa; identical to C-terminal region previously sequenced SW:DNAA_STRCO (EMBL:M82836) Streptomyces coelicolor chromosomal replication initiator protein DnaA, 656 aa. Contains Pfam match to entry PF00308 bac_dnaA, Bacterial dnaA protein and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS01008 DnaA protein signature. Contains also a 5x degenerate repeat: (D/E)Y(D/E)GR,StH24.01c, dnaA, chromosomal replication initiator protein, partial CDS, len >81 aa; previously sequenced as SW:DNAA_STRCO (EMBL:M82836), DnaA 2 3 6 8 [Reporter]SCO3717 (14N14)_PCR/4653 [Gene]SCO3717/4243 NC_003888 SCH35.07, probable cation transport system component, len: 707 aa; similar to many e.g. SW:ATKB_ECOLI AtkB/KdpB potassium transporting ATPase A chain from Escherichia coli (682 aa) fasta scores; opt: 2748, z-score: 2854.0, E(): 0, (65.1% identity in 676 aa overlap). Contains Pfam match to entry PF00122 E1-E2_ATPase, E1-E2 ATPases and Prosite matches to PS00154 E1-E2 ATPases phosphorylation site and PS00018 EF-hand calcium-binding domain. Contains possible membrane spanning hydrophobic regions. 1 2 12 10 [Reporter]SCO2837 (12M23)_PCR/2267 [Gene]SCO2837/2069 NC_003888 SCE20.11, possible secreted protein, len: 645 aa. Weakly similar to a Eukaryotic galactose oxidase precursor (EC 1.1.3.9) from Dactylium dendroides (Cladobotryum dendroides) SW:GAOA_DACDE(EMBL:M86819) (680 aa), fasta scores opt: 350 z-score: 374.4 E(): 1.8e-13 26.8% identity in 400 aa overlap. Contains a possible N-terminal signal sequence. 2 3 6 7 [Reporter]SCO1629 (15J14)_PCR/4652 [Gene]SCO1629/4242 NC_003888 SCI41.12c, cvnB9, unknown, len: 135 aa. Similar to TR:CAB41561 (EMBL:AL049727) Streptomyces coelicolor SC9B1.15C hypothetical 14.3 kD protein, 137 aa; fasta scores: opt: 266 z-score: 339.1 E(): 1.6e-11; 37.0% identity in 127 aa overlap and to TR:Q9AJP3 (EMBL:AB044803) Streptomyces girseus ORF2, 135 aa; fasta scores: opt: 559 Z-score: 671.5 E(): 9e-30; 62.687% identity in 134 aa overlap 1 2 12 9 [Reporter]SCO0804 (13I23)_PCR/2266 [Gene]SCO0804/2068 NC_003888 SCF43.15c, unknown, len: 247 aa. Weakly similar to several proteins of undefined function e.g. Pyrococcus horikoshii TR:O58235(EMBL:AP000002) (272 aa) fasta scores opt:323 z-score: 406.6 E(): 2.9e-15 28.2% identity in 227 aa overlap. 2 3 6 6 [Reporter]SCO1715 (16F14)_PCR/4651 [Gene]SCO1715/4241 NC_003888 SCI11.04, hgd, probable homogentisate 1,2-dioxygenase, len: 441 aa; highly similar to eukaryotic homogentisate 1,2-dioxygenases e.g. SW:HGD_HUMAN (EMBL:U63008), hgd, Homo sapiens homogentisate 1,2-dioxygenase (445 aa), fasta scores; opt: 1551 z-score: 1809.0 E(): 0, 52.1% identity in 436 aa overlap 1 1 18 12 [Reporter]SCO4398 (11M8)_PCR/385 [Gene]SCO4398/349 NC_003888 SCD10.30c, conserved hypothetical protein, len: 217 aa; similar to TR:Q9ZBP8 (EMBL:AL034447) Streptomyces coelicolor hypothetical 23.3 kD protein SC7A1.25c, 223 aa; fasta scores: opt: 514 z-score: 621.6 E(): 3.8e-27; 45.0% identity in 189 aa overlap 1 2 12 8 [Reporter]SCO3746 (14E23)_PCR/2265 [Gene]SCO3746/2067 NC_003888 SCH22A.24c, putative membrane protein, len: 412 aa; similar to TR:P96400 (EMBL:Z92669) Mycobacterium tuberculosis hypothetical 47.4 kD protein, 442 aa; fasta scores: opt: 728 z-score: 812.0 E(): 0; 52.1% identity in 411 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 6 5 [Reporter]SCO3212 (17B14)_PCR/4650 [Gene]SCO3212/4240 NC_003888 SCE8.05c, trpD2, anthranilate phosphoribotransferase, len: 335 aa; similar to many e.g. SW:TRPD_AZOBR TrpD, anthranilate phosphoribotransferase from Azospirillum brasilense (355 aa) fasta scores; opt: 924, z-score: 1056.6, E(): 0, (49.5% identity in 315 aa overlap). Contains Pfam match to entry PF00591 Glycos_transf_3, glycosyl transferase family, score 223.70, E-value 2.8e-63. 1 1 18 11 [Reporter]SCO4633 (12I8)_PCR/384 [Gene]SCO4633/348 NC_003888 SCD82.04c, hypothetical protein, len: 218 aa; similar to various hypothetical proteins, e.g. TR:O07609 (EMBL:Y14083) Bacillus subtilis hypothetical 22.8 kD protein YhfK, 214 aa; fsta scores: opt: 425 z-score: 473.2 E(): 6.1e-19; 36.6% identity in 216 aa overlap 1 2 12 7 [Reporter]SCO3868 (15A23)_PCR/2264 [Gene]SCO3868/2066 NC_003888 SCH18.05, unknown, len: 208 aa 1 1 18 10 [Reporter]SCO3234 (13E8)_PCR/383 [Gene]SCO3234/347 NC_003888 SCE29.03, possible phosphotransferase, len: 300 aa; weak similarity to TR:P72441 (EMBL:U70376) spectinomycin phosphotransferase from spectinomycin producer Streptomyces flavopersicus (330 aa) fasta scores; opt: 145, z-score: 177.0, E(): 0.015, (25.3% identity in 261 aa overlap). 1 2 12 6 [Reporter]SCO0337 (16M19)_PCR/2263 [Gene]SCO0337/2065 NC_003888 SCF12.16, probable transcriptional regulator, len: 188 aa; similar to many transcriptional regulators from Streptomyces spp. e.g. SW:TCMR_STRGA tetracenomycin C transcriptional repressor from Streptomyces glaucescens (226 aa) fasta scores; opt: 152, z-score: 176.0, E(): 0.019, 28.1% identity in 203 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a possible helix-turn-helix motif (+3.98 SD) 29-50aa. 1 1 18 9 [Reporter]SCO3358 (14A8)_PCR/382 [Gene]SCO3358/346 NC_003888 SCE94.09, cseB, two-component system response regulator, len: 234 aa; previously sequenced therefore identical to TR:E1358527 (EMBL:AJ131213) CseB, response regulator, positive regulator of sigE transcription from Streptomyces coelicolor (234 aa) fasta scores; opt: 1529, z-score: 1731.2, E(): 0, (100.0% identity in 234 aa overlap). Also similar to many others eg. SW:PHOP_BACSU PhoP, response regulator of the phosphate regulon of Bacillus subtilis (240 aa) fasta scores; opt: 582, z-score: 665.4, E(): 9.4e-30, (41.2% identity in 233 aa overlap). Contains Pfam matches to entry PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal and entry PF00072 response_reg, Response regulator receiver domain. 1 2 12 5 [Reporter]SCO0079 (17I19)_PCR/2262 [Gene]SCO0079/2064 NC_003888 SCJ11.08c, possible integral membrane transport protein, len: 407 aa; similar to many e.g. TR:P72472 (EMBL:U63848) sensor protein homolog from Streptomyces lividans (410 aa) fasta scores; opt: 2533, z-score: 2742.9, E(): 0, (98.5% identity in 407 aa overlap) and TR:Q56147 (EMBL:U09991) chloramphenicol resistance protein from Streptomyces venezuelae (436 aa) fasta scores; opt: 559, z-score: 610.6, E(): 1.2e-26, (28.2% identity in 411 aa overlap). Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter. 1 1 18 8 [Reporter]SCO0466 (15M4)_PCR/381 [Gene]SCO0466/345 NC_003888 SCF76.06, probable araC family transcriptional regulator, len: 327 aa. Highly similar to many including the Streptomyces coelicolor hypothetical regulators: TR:CAB40696 (EMBL; AL049587) SC5F2A.29 (325 aa), fasta scores opt: 857 z-score: 962.2 E():0 45.8% identity in 323 aa overlap and TR:CAB46927 (EMBL; AL096822) SCGD3.05 (334 aa), fasta scores opt: 617 z-score: 694.7 E(): 2.4e-31 38.6% identity in 332 aa overlap. In addition to Streptomyces lividans SW:YCP1_STRLI (EMBL; U02635) hypothetical protein in CPOL 5'region (ORF1) (fragment) (141 aa), fasta scores opt: 908 z-score: 1024.0 E():0 99.3% identity in 141 aa overlap. Contains a Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. There are two possible helix-turn-helix motifs located between residues 237..258 (+3.99 SD) and 301..322 (+2.58 SD). Note that the translational start site may be 'M' residue 6. 1 2 12 4 [Reporter]SCO3681 (18E19)_PCR/2261 [Gene]SCO3681/2063 NC_003888 SCH35.43c, unknown, len: 166aa; similar to TR:Q9X8W6 (EMBL:AL078610) SCH35.40 hypothetical protein from Streptomyces coelicolor. 1 1 18 7 [Reporter]SCO0445 (16I4)_PCR/380 [Gene]SCO0445/344 NC_003888 SCF51A.23c, possible secreted protein, len: 405 aa. Contains a possible N-terminal signal sequence. 1 2 12 3 [Reporter]SCO6296 (19A19)_PCR/2260 [Gene]SCO6296/2062 NC_003888 SCBAC8D1.09c, conserved hypothetical protein, len: 159 aa; similar to TR:Q9HTH9 (EMBL:AE004951) Pseudomonas aeruginosa hypothetical protein PA5385, 159 aa; fasta scores: opt: 363 Z-score: 448.1 bits: 89.1 E(): 2.3e-17; 40.764% identity in 157 aa overlap 1 1 18 6 [Reporter]SCO0180 (17E4)_PCR/379 [Gene]SCO0180/343 NC_003888 SCJ1.29c, unknown, len: 283 aa. Similar to many including: Mycobacterium tuberculosis TR:O53472 (EMBL; AL021899) hypothetical 31.5 KD protein (294 aa), fasta scores opt: 329 z-score: 389.0 E(): 2.6e-14 32.1% identity in 293 aa overlap and Mycobacterium tuberculosis TR:O06189 (EMBL; Z95387) hypothetical 31.7 KD protein (297 aa), fasta scores opt: 330 z-score: 390.1 E(): 2.3e-14 31.8% identity in 296 aa overlap. Also similar to S. coelicolor SCJ1.16c (294 aa), fasta scores opt: 806 z-score: 748.1 E():0 46.1% identity in 295 aa overlap. Contains a Pfam match to entry PF00582 Usp, Universal stress protein family 1 2 12 2 [Reporter]SCO5067 (20I15)_PCR/2259 [Gene]SCO5067/2061 NC_003888 SCBAC20F6.10, probable carboxylesterase, len: 497 aa; similar to SW:PNBA_BACSU (EMBL:U06089) Bacillus subtilis para-nitrobenzyl esterase (EC 3.1.1.*) PnbA, 489 aa; fasta scores: opt: 784 Z-score: 827.6 bits: 162.6 E(): 1.6e-38; 37.205% identity in 508 aa overlap. Contains 2x Pfam matches to entry PF00135 COesterase, Carboxylesterase and match to Prosite entry PS00122 Carboxylesterases type-B serine active site 1 1 18 5 [Reporter]SCO3373 (18A4)_PCR/378 [Gene]SCO3373/342 NC_003888 SCE94.24c, probable Clp-family ATP-binding protease, len: 841aa; highly similar to many egs. SW:MECB_BACSU MecB/ClpC pleiotropic regulator controlling competence gene expression and growth at high temperature in Bacillus subtilis (810 aa) fasta scores; opt: 3266, z-score: 3278.7, E(): 0, (61.6% identity in 805 aa overlap) and TR:Q48760 (EMBL:U40604) ClpC ATPase required for stress tolerance and in vivo survival of Listeria monocytogenes (825 aa) fasta scores; opt: 3204, z-score: 3216.3, E(): 0, (61.2% identity in 804 aa overlap). Also similar to TR:O69936 (EMBL:AL023861) clp protease ATP binding subunit from Streptomyces coelicolor (842 aa) fasta scores; opt: 2357, z-score: 2283.1, E(): 0, (48.9% identity in 829 aa overlap). Contains Pfam match to entry PF00495 clpA_B, Chaperonin clpA/B and two Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 2 11 22 [Reporter]SCO1261 (1I11)_PCR/2257 [Gene]SCO1261/2060 NC_003888 2SCG18.08, probable transcriptional regulatory protein, len: 384 aa; similar to TR:Q9S2D8 (EMBL:AL110470) Streptomyces coelicolor putative transcriptional repressor protein SCF85.03, 385 aa; fasta scores: opt: 980 z-score: 1062.5 E(): 0; 44.2% identity in 380 aa overlap and to SW:XYLR_ANATH (EMBL:Z69782) Anaerocellum thermophilum xylose repressor XylR, 399 aa; fasta scores: opt: 376 z-score: 411.5 E(): 1.9e-15; 25.4% identity in 394 aa overlap. Contains Pfam match to entry PF00480 ROK, ROK family and possible helix-turn-helix motif at residues 30..51 (+2.57 SD) 1 1 18 4 [Reporter]SCO1968 (18M24)_PCR/377 [Gene]SCO1968/341 NC_003888 SC3C9.03, probable secreted hydrolase, len: 289 aa; similar to SW:GLPQ_BACSU (EMBL:Z26522) Bacillus subtilis glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46) GlpQ, 293 aa; fasta scores: opt: 495 Z-score: 576.9 bits: 114.7 E(): 1.6e-24; 34.114% identity in 299 aa overlap. Contains possible cleavable N-terminal region signal peptide sequence 1 1 18 3 [Reporter]SCO1376 (19I24)_PCR/376 [Gene]SCO1376/340 NC_003888 SC10A9.18, probable lacI-family transcriptional regulatory protein, len: 356 aa; similar to SW:CELR_THEFU (EMBL:AF086819) Thermomonospora fusca transcription regulator CelR, 340 aa; fasta scores: opt: 681 z-score: 700.7 E(): 1.4e-31; 40.7% identity in 339 aa overlap and to TR:O86795 (EMBL:AL031317) Streptomyces coelicolor probable transcriptional regulator SC6G4.32, 355 aa; fasta scores: opt: 1196 z-score: 1059.6 E(): 0; 56.7% identity in 344 aa overlap. Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and match to Prosite entry PS00356 Bacterial regulatory proteins, lacI family signature. Also contains a possible helix-turn-helix motif at residues 12..39 (+7.22 SD) 2 4 23 9 [Reporter]SCO5594 (14F9)_PCR/7042 [Gene]SCO5594/6419 NC_003888 SC2E1.11, trmD, probable tRNA (guanine-N1)-methyltransferase, len: 277 aa; similar to many e.g. TRMD_ECOLI (EC2.1.1.31) (255 aa), fasta scores; opt: 437 z-score: 660.6E(): 1.5e-29, 41.2% identity in 238 aa overlap 2 4 23 8 [Reporter]SCO3566 (15B9)_PCR/7041 [Gene]SCO3566/6418 NC_003888 SCH5.29, possible hydrolase, len: 324aa; similar to many egs. TR:O69638 (EMBL:AL022121) putative epoxide hydrolase from Mycobacterium tuberculosis (327 aa) fasta scores; opt: 687, z-score: 786.1, E(): 0, (40.7% identity in 327 aa overlap) and TR:Q39856 (EMBL:D63781) epoxide hydrolase from Glycine max (soybean) (341 aa) fasta scores; opt: 396, z-score: 455.4, E(): 4.6e-18, (29.1% identity in 316 aa overlap). Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold, score 68.70, E-value 1.3e-16. 2 4 23 7 [Reporter]SCO3849 (16N5)_PCR/7040 [Gene]SCO3849/6417 NC_003888 SCH69.19c, possible integral membrane protein, len: 253 aa; contains possible membrane spanning hydrophobic region. Similar to neighbouring gene SCH69.20c fasta scores; opt: 651, z-score: 689.2, E(): 2.8e-33, (45.9% identity in 218 aa overlap) 2 4 23 6 [Reporter]SCO0029 (17J5)_PCR/7039 [Gene]SCO0029/6416 NC_003888 SCJ4.10, unknown, len: 99 aa 2 4 23 5 [Reporter]SCO1701 (18F5)_PCR/7038 [Gene]SCO1701/6415 NC_003888 SCI30A.22c, probable acyl-CoA dehydrogenase, len: 383 aa; similar to many both prokaryote and eukaryote eg. SW:ACDB_BACSU acyl-CoA dehydrogenase from Bacillus subtilis (379 aa) fasta scores; opt: 109,1 z-score: 1197.8, E(): 0, (43.8% identity in 377 aa overlap) and SW:ACDS_RAT acyl-CoA dehydrogenase from Rattus norvegicus (Rat) (412 aa) fasta scores; opt: 1079, z-score: 1184.2, E(): 0, (44.6% identity in 372 aa overlap). Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase. 2 4 23 4 [Reporter]SCO4892 (19B5)_PCR/7037 [Gene]SCO4892/6414 NC_003888 2SCK8.18, possible regulatory protein, len: 340 aa; similar to TR:Q9XBC8 (EMBL:AL078635) Amycolatopsis orientalis putative transcriptional regulator CZA382.23c, 335 aa; fasta scores: opt: 222 z-score: 228.5 E(): 3.6e-05; 33.0% identity in 345 aa overlap 2 4 23 3 [Reporter]SCO4013 (20N1)_PCR/7036 [Gene]SCO4013/6413 NC_003888 2SC10A7.17, probable secreted penicillin-binding protein, len: 495 aa; similar to TR:Q9XA17 (EMBL:AL079308) Streptomyces coelicolor putative secreted penicillin-binding protein SCH69.17, 490 aa; fasta scores: opt: 1733 z-score: 1829.7 E(): 0; 55.0% identity in 498 aa overlap and to TR:AAF61246 (EMBL:AF241575) Streptomyces griseus PbpA, 485 aa; fasta scores: opt: 1186 z-score: 1253.7 E(): 0; 55.8% identity in 495 aa overlap. Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Also contains possible N-terminal region signal peptide sequence 2 4 22 22 [Reporter]SCO1140 (1F21)_PCR/7034 [Gene]SCO1140/6412 NC_003888 2SCG38.33, possible integral membrane protein, len: 499 aa; similar to SW:YZ2R_AGRVI (EMBL:U32375) Agrobacterium vitis hypothetical 52.8 kDa protein, 502 aa; fasta scores: opt: 1419 z-score: 1523.5 E(): 0; 44.1% identity in 479 aa overlap. Contains Pfam match to entry PF01970 DUF112, Integral membrane protein DUF112. Also contains possible hydrophobic membrane spanning regions 2 3 6 4 [Reporter]SCO0898 (18N10)_PCR/4649 [Gene]SCO0898/4239 NC_003888 SCM1.31c, possible regulator, len: 660 aa. Similar to many other regulators including several from Streptomyces coelicolor e.g. SW:AFSR_STRCO (EMBL:D90155) regulatory protein AfsR (993 aa), fasta scores opt: 531 z-score: 560.0 E(): 7.5e-24 31.9% identity in 568 aa overlap and TR:Q9Z389(EMBL:AL035654) putative transcriptional regulator SCE8.10 (638 aa), fasta scores opt: 430 z-score: 456.1 E(): 4.6e-18 31.2% identity in 385 aa overlap. 2 4 22 21 [Reporter]SCO6499 (2B21)_PCR/7033 [Gene]SCO6499/6411 NC_003888 SC1E6.08, gvpO, probable gas vesicle synthesis protein, len: 130 aa; similar to several from Halobacteria e.g. GVO1_HALSA GVPO protein, plasmid (120 aa), fasta scores; opt: 167 z-score: 285.9 E(): 1.2e-08, 30.2% identity in 106 aa overlap. Contains 9x degenerate QXS repeat near N-terminus and apparent helix-turn-helix motif at aa 53-74 (Score 1244, +3.42 SD) 2 3 6 3 [Reporter]SCP1.10 (19J10)_PCR/4648 [Gene]SCP1.10/4238 NC_003888 SCP1.10, unknown, contains TTA encoded leucine at residue 33, possible target for bldA regulation. len: 164aa; 2 4 22 20 [Reporter]SCO6416 (3N17)_PCR/7032 [Gene]SCO6416/6410 NC_003888 SC1A6.05, unknown, len: 340 aa; some similarity to e.g. TR:Q49598 (EMBL:U31567) Methanopyrus kandleri N5,N10-methylenetetrahydromethanopterin reductase (349 aa), fasta scores; opt: 207 z-score: 425.6 E(): 1.4e-16, 25.7% identityin 358 aa overlap 2 3 6 2 [Reporter]SCO4990 (20B10)_PCR/4647 [Gene]SCO4990/4237 NC_003888 2SCK36.13, conserved hypothetical protein, len: 136 aa; similar to SW:YABJ_BACSU (EMBL:D26185) Bacillus subtilis putative regulator of purine biosynthesis YabJ, 124 aa; fasta scores: opt: 239 Z-score: 301.4 bits: 61.4 E(): 3.5e-09; 36.641% identity in 131 aa overlap. Contains Pfam match to entry PF01042 UPF0076, YjgF family 2 3 5 22 [Reporter]SCO5386 (1B6)_PCR/4645 [Gene]SCO5386/4236 NC_003888 2SC6G5.30, possible anti-sigma factor antagonist, len: 107aa; similar to many eg. SW:P95842 (RSBV_STAAU) anti-sigma B factor antagonist from Staphylococcus aureus (108 aa) fasta scores; opt: 164, z-score: 226.7, E(): 3.7e-05, 30.9% identity in 94 aa overlap. Contains Pfam match to entry PF01740 SpoIIAA, SpoIIAA family. 2 3 5 21 [Reporter]SCO3203 (2N2)_PCR/4644 [Gene]SCO3203/4235 NC_003888 SCE22.20, bar, phosphinothricin acetyltransferase, len: 171 aa; identical to previously sequenced SW:BAR_STRCO (EMBL:M62753) Streptomyces coelicolor phosphinothricin acetyltransferase (EC 2.3.1.-) Bar, 171 aa. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 2 3 5 20 [Reporter]SCO1440 (3J2)_PCR/4643 [Gene]SCO1440/4234 NC_003888 SC6D7A.03c, ribH, 6,7-dimethyl-8-ribityllumazine synthase, len: 161 aa; highly similar to SW:RISB_CORAM (EMBL:AB003693) Corynebacterium ammoniagenes 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.9) RibH, 163 aa; fasta scores: opt: 456 z-score: 563.8 E(): 7.2e-24; 46.8% identity in 158 aa overlap. Contains Pfam match to entry PF00885 DMRL_synthase, 6,7-dimethyl-8-ribityllumazine synthase 2 3 5 19 [Reporter]SCO6897 (4F2)_PCR/4642 [Gene]SCO6897/4233 NC_003888 SC1B2.03, unknown, len: 103 aa. 1 2 11 21 [Reporter]SCO1576 (2E11)_PCR/2256 [Gene]SCO1576/2059 NC_003888 SCL24.12c, argR, arginine repressor, len: 179 aa; identical to previously sequenced SW:ARGR_STRCL (EMBL:Y11134) Streptomyces clavuligerus arginine repressor ArgR, 160 aa. Contains Pfam match to entry PF01316 Arg_repressor, Arginine repressor 2 3 5 18 [Reporter]SCO6335 (5B2)_PCR/4641 [Gene]SCO6335/4232 NC_003888 SC3A7.03c, questionable ORF, len: 304 aa; this ORF is not predicted as coding by positional base preference 1 2 11 20 [Reporter]SCO1484 (3A11)_PCR/2255 [Gene]SCO1484/2058 NC_003888 SC9C5.08c, pyrAA, carbamoyl-phosphate synthase, pyrimidine-specific, small chain, len: 380 aa; similar to SW:CARA_BACCL (EMBL:X73308) Bacillus caldolyticus carbamoyl-phosphate synthase, pyrimidine-specific, small chain (EC 6.3.5.5) PyrAA, 364 aa; fasta scores: opt: 826 z-score: 913.3 E(): 0; 45.3% identity in 362 aa overlap. Contains Pfam matches to entries PF00988 CPSase_sm_chain, Carbamoyl-phosphate synthase small chain, CPSase domain and PF00117 GATase, Glutamine amidotransferase class-I and match to Prosite entry PS00442 Glutamine amidotransferases class-I active site 2 3 5 17 [Reporter]SCO7061 (5N22)_PCR/4640 [Gene]SCO7061/4231 NC_003888 SC4G1.27c, probable marR-family transcriptional regulator, len: 178 aa; similar to SW:YXAD_BACSU (EMBL:AB005554) Bacillus subtilis hypothetical transcriptional regulator in AsnH-GntR intergenic region YxaD, 143 aa; fasta scores: opt: 242 z-score: 315.6 E(): 4.1e-10; 36.4% identity in 132 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family and possible helix-turn-helix motif at residues 65..86 (+3.24 SD) 1 1 18 2 [Reporter]SCO5114 (20A24)_PCR/375 [Gene]SCO5114/339 NC_003888 SCBAC31E11.10, bldKC, possible ABC transport system integral membrane protein, len: 325 aa; almost identical to previously sequenced TR:P72408 (EMBL:U68036) Streptomyces coelicolor BldKC, 323 aa. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Also contains possible hydrophobic membrane spanning regions 1 2 11 19 [Reporter]SCO2248 (4M7)_PCR/2254 [Gene]SCO2248/2057 NC_003888 SC1G2.10, hypothetical protein, len: 165 aa; similar to TR:Q9RD82 (EMBL:AL136502) Streptomyces coelicolor hypothetical protein SCF43.06, 164 aa; fasta scores: opt: 669 Z-score: 833.5 E(): 8.6e-39; 60.736% identity in 163 aa overlap 2 3 5 16 [Reporter]SCO7321 (6J22)_PCR/4639 [Gene]SCO7321/4230 NC_003888 SC5F8.31c, unknown, len: 354 aa. Similar to Deinococcus radiodurans TR:Q9RZT6(EMBL:AE001826) hypothetical TorS-related protein (351 aa), fasta scores opt: 393 z-score: 443.7 E(): 3e-17 34.7% identity in 354 aa overlap. 1 1 17 22 [Reporter]SCO1127 (1A20)_PCR/373 [Gene]SCO1127/338 NC_003888 2SCG38.20, probable oxidoreductase, len: 312 aa; similar to SW:BDHA_RHIME (EMBL:AF080548) Rhizobium meliloti D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) BdhA, 258 aa; fasta scores: opt: 332 z-score: 358.5 E(): 1.6e-12; 32.7% identity in 223 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 1 2 11 18 [Reporter]SCO2202 (5I7)_PCR/2253 [Gene]SCO2202/2056 NC_003888 SC3H12.10 htpX2, protease HtpX homologue (heat shock protein) (putative membrane protein), len: 304 aa; identical to previously sequenced TR:CAB58284 (EMBL:AL121850) Streptomyces coelicolor protease HtpX homolog (EC 3.4.24.-) HtpX, 304 aa and similar to SW:HTPX_METJA (EMBL:U67608) Methanococcus jannaschii probable protease HtpX homolog (EC 3.4.24.-) MJ1682, 284 aa; fasta scores: opt: 568 z-score: 668.3 E(): 7.7e-30; 36.8% identity in 288 aa overlap. Contains Pfam match to entry PF01435 Peptidase_M48, Peptidase family M48 and possible hydrophobic membrane spanning regions 1 1 17 21 [Reporter]SCO0434 (2M16)_PCR/372 [Gene]SCO0434/337 NC_003888 SCF51A.12, malX, sugar phosphotransferase, len: 549 aa. Highly similar to Escherichia coli SW:PTOA_ECOLI (EMBL; M60722) PTS system, maltose and glucose-specific II ABC component (maltose and glucose-permease II ABC component) (phosphotransferase enzyme II, ABC component) (EC 2.7.1.69) (523 aa), fasta scores opt: 1852 z-score: 2064.1 E():0 59.5% identity in 543 aa overlap. Contains a Pfam match to entry PF00367 PTS_EIIB, phosphotransferase system, EIIB and a Prosite hit to PS01035 PTS EIIB domains cysteine phosphorylation site signature. Contains multiple possible membrane spanning hydrophobic domains 1 2 11 17 [Reporter]SCO2052 (6E7)_PCR/2252 [Gene]SCO2052/2055 NC_003888 SC4G6.21c, hisB, imidazoleglycerol-phosphate dehydratase, len: 197aa; previously sequenced therefore identical to SW:HIS7_STRCO. Contains Pfam match to entry PF00475 IGPD, Imidazoleglycerol-phosphate dehydratase and Prosite matches to PS00955 Imidazoleglycerol-phosphate dehydratase signature 2 and PS00954 Imidazoleglycerol-phosphate dehydratase signature 1. 1 1 17 20 [Reporter]SCO6821 (3I16)_PCR/371 [Gene]SCO6821/336 NC_003888 SC1A2.30c, possible transferase, len: 240 aa. Weakly similar to the C-terminal glutamine amide transferase domain of several CTP synthases e.g. Escherichia coli SW:PYRG_ECOLI(EMBL:M12843) CTP synthase (EC 6.3.4.2) (544 aa), fasta scores opt: 184 z-score: 227.2 E(): 3e-05 24.4% identity in 225 aa overlap. 1 2 11 16 [Reporter]SCO7269 (7A7)_PCR/2251 [Gene]SCO7269/2054 NC_003888 SC5H1.23, hypothetical protein, len: 171 aa; unknown function, similar to TR:O53728 (EMBL:AL021932) Mycobacterium tuberculosis hypothetical protein (171 aa), fasta scores; opt: 561 z-score: 716.6 E(): 1.4e-32, 49.7% identity in 165 aa overlap 1 1 17 19 [Reporter]SCO2107 (4E16)_PCR/370 [Gene]SCO2107/335 NC_003888 SC6E10.01, possible oxidoreductase, partial CDS, len: >375 aa; similar to TR:O85228 (EMBL:AF053760), HcnC, Pseudomonas fluorescens hydrogen cyanide synthase (417 aa), fasta scores; opt: 511 z-score: 564.8 E(): 3.9e-24, 30.7% identity in 348 aa overlap, although they are more divergent at the N-terminus. Also similar to many other oxidoreductases and hypothetical proteins,SC2C1A.03, possible oxidoreductase, partial CDS, len: > 40 aa. Similar to Bacillus subtilis SW:DLD2_BACSU(EMBL:D84432) dihydrolipoamide dehydrogenase (EC 1.8.1.4) (474 aa), fasta scores opt: 111 z-score: 145.4 E(): 0.94 54.3% identity in 35 aa overlap. Overlaps and extends into CDS SC6E10.01 on the adjoining cosmid 1 2 11 15 [Reporter]SCO5578 (8M3)_PCR/2250 [Gene]SCO5578/2053 NC_003888 SC7A1.22, sugar transporter, len: aa; similar to many eg. SW:GLCP_SYNY3 glucose transport protein from Synechocystis sp. (468 aa) fasta scores; opt: 1477, z-score: 1527.5, E(): 0, (50.6% identity in 468 aa overlap). Contains PS00217 Sugar transport proteins signature 2, PS00216 Sugar transport proteins signature 1 and Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter, score 404.10, E-value 1.3e-117. Also contains several possible transmembrane regions. 1 1 17 18 [Reporter]SCO2395 (5A16)_PCR/369 [Gene]SCO2395/334 NC_003888 SC4A7.23c, possible integral membrane protein, len: 274 aa; low similarities to SW:CYSZ_ECOLI (EMBL:AE000329) Escheichia coli CysZ protein, 253 aa; fasta scores: opt: 258 z-score: 295.2 E(): 4.6e-09; 25.8% identity in 244 aa overlap 1 2 11 14 [Reporter]SCO2656 (9I3)_PCR/2249 [Gene]SCO2656/2052 NC_003888 SC8E4A.26, possible membrane protein, len: 408 aa; similar to various hypothetical proteins, e.g. TR:Q9XA89 (EMBL:AL096837) Streptomyces coelicolor hypothetical 36.1 kD protein SCF43A.26c, 333 aa; fasta scores: opt: 661 z-score: 668.4 E(): 8.1e-30; 42.3% identity in 317 aa overlap. Contains Pfam match to entry PF00924 UPF0003, Uncharacterized protein family UPF0003 and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Contains also possible hydrophobic membrane spanning regions 1 1 17 17 [Reporter]SCO6542 (6M12)_PCR/368 [Gene]SCO6542/333 NC_003888 SC5C7.27, possible secreted glycosyl hydrolase len: 631 aa; similar to two hypothetical proteins from B. subtilis TR:O31526 (EMBL:Z99107) yesW (620 aa), fasta scores; opt: 2404 z-score: 2009.8 E(): 0, 57.7% identity in 620 aa overlap and TR:O31527 (EMBL:Z99107) yesX (612 aa), fasta scores; opt: 1856 z-score: 1984.4 E(): 0, 54.4% identity in 599 aa overlap. Contains possible N-terminal signal sequence. Similar to TR:Q9AF09 (EMBL:AY026755) rhamnogalacturonan lyase from Pseudomonas cellulosa (881 aa) fasta scores; opt: 2392, Z-score: 2482.1, 59.091% identity (61.043% ungapped) in 594 aa overlap. 1 2 11 13 [Reporter]SCO5118 (10E3)_PCR/2248 [Gene]SCO5118/2051 NC_003888 SC9E12.03, probable peptide transport system integral membrane protein, len: 307 aa; similar to TR: SW:OPPB_BACSU (EMBL:X56347) Bacillus subtilis oligopeptide transport system permease protein OppB, 311 aa; fasta scores: opt: 688 z-score: 788.7 E(): 0; 36.0% identity in 308 aa overlap and to TR:P72408 (EMBL:U68036) Streptomyces coelicolor SpoOKC homologue BldKC, 323 aa; blastp scores: Score = 354 (124.6 bits), Expect = 1.9e-32, P = 1.9e-32; Identities = 82/235 (34%), Positives = 138/235 (58%). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Also contains possible hydrophobic membrane spanning regions 1 1 17 16 [Reporter]SCO5656 (7I12)_PCR/367 [Gene]SCO5656/332 NC_003888 SC6A9.11, probable transcriptional regulatory protein, len: 166 aa; similar to many members of the asnC family e.g. LRP_ECOLI leucine-responsive regulatory protein (164 aa), fasta scores; opt: 190 z-score: 294.3 E(): 3.7e-09, 27.6% identity in 156 aa overlap. Contains PS00519 Bacterial regulatory proteins, asnC family signature and probable helix-turn-helix motif at aa 39-60 (Score 1545, +4.45 SD) 1 2 11 12 [Reporter]SCO2258 (11A3)_PCR/2247 [Gene]SCO2258/2050 NC_003888 SCC75A.04, probable ABC-transporter, transmembrane component, len: 274 aa. Similar to many transport proteins including: Streptomyces peucetius SW:DRRB_STRPE(EMBL:M73758) daunorubicin resistance transmembrane protein (283 aa), fasta scores opt: 523 z-score: 593.2 E(): 1.2e-25 35.1% identity in 245 aa overlap and Streptomyces antibioticus TR:Q53717(EMBL:L06249) membrane protein OLEC-ORF5 involved in oleandomycin-resistance (273 aa), fasta scores opt: 1502 z-score: 1685.2 E():0 83.2% identity in 274 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 1 1 17 15 [Reporter]SCO5291 (8E12)_PCR/366 [Gene]SCO5291/331 NC_003888 SC6G9.42c, cvnC5, conserved hypothetical protein, len: 150 aa; similar to hypothetical proteins of varying length (divergent at the N-terminus) from S. coelicolor and Mycobacterium tuberculosis e.g. TR:O86523 (EMBL:AL031124) S.coelicolor hypothetical protein (132 aa), fasta scores; opt: 250 z-score: 312.1 E(): 4.7e-10, 43.7% identity in 135 aa overlap and TR:O50392 (EMBL:AL009198) Mycobacterium tuberculosis hypothetical protein (122 aa) (37.4% identity in 131 aa overlap) 1 1 17 14 [Reporter]SCO2627 (9A12)_PCR/365 [Gene]SCO2627/330 NC_003888 SC8E4.02c, probable sugar-phosphate isomerase, len: 159 aa; similar to TR:O53192 (EMBL:AL021246) Mycobacterium tuberculosis putative isomerase MTV008.21c, 162 aa; fasta scores: opt: 665 z-score: 811.6 E(): 0; 62.1% identity in 153 aa overlap and to SW:RPIB_ECOLI (EMBL:X82203) Escherichia coli ribose 5-phosphate isomerase B (EC 5.3.1.6) (phosphoriboisomerase B) RpiB, 149 aa; fasta scores: opt: 294 z-score: 366.6 E(): 5.2e-13; 37.5% identity in 144 aa overlap 2 4 22 19 [Reporter]SCO7231 (4J17)_PCR/7031 [Gene]SCO7231/6409 NC_003888 SC2H12.30, possible two-component system sensor kinase, len: 503 aa. Highly similar to many other sensor kinases e.g. Streptomyces coelicolor SW:AFQ2_STRCO (EMBL:D10654) sensor protein, AfsQ2, a putative regulator of secondary metabolism (EC 2.7.3.-) (535 aa), fasta scores opt: 1112 z-score: 1254.8 E():0 42.0% identity in 519 aa overlap. Contains Pfam matches to entries PF00672 DUF5, Domain found in bacterial signal proteins and PF00512 signal, Histidine kinase. Contains possible membrane spanning hydrophobic domains. 2 4 22 18 [Reporter]SCO2408 (5F17)_PCR/7030 [Gene]SCO2408/6408 NC_003888 SC4A7.36, possible aminotransferase, len: 541 aa; similar to SW:HIS8_METJA (EMBL:U67539) Methanococcus jannaschii probable histidinol-phosphate aminotransferase (EC 2.6.1.9) HisC, 373 aa; fasta scores: opt: 390 z-score: 299.5 E(): 2.6e-09; 25.1% identity in 334 aa overlap. Contains Pfam match to entry PF00222 aminotran_2, Aminotransferases class-II 2 4 22 17 [Reporter]SCO7788 (6B17)_PCR/7029 [Gene]SCO7788/6407 NC_003888 SC5E9.36c, putative membrane protein, len: 65 aa. Contains possible hydrophobic membrane spanning regions 2 4 22 16 [Reporter]SCO1421 (7N13)_PCR/7028 [Gene]SCO1421/6406 NC_003888 SC6D7.18c, unknown, len: 124 aa. Highly similar to several including: Streptomyces coelicolor TR:Q9Z522(EMBL:AL035591) putative electron transport protein SCC54.03C (111 aa), fasta scores opt: 223 z-score: 290.6 E(): 8.2e-09 33.6% identity in 107 aa overlap and Mycobacterium tuberculosis TR:O53492(EMBL:AL021899) hypothetical 13.0 KD protein (111 aa), fasta scores opt: 426 z-score: 539.6 E(): 1.1e-22 57.4% identity in 108 aa overlap. 2 4 22 15 [Reporter]SCO5956 (8J13)_PCR/7027 [Gene]SCO5956/6405 NC_003888 SC7H1.26, possible regulatory protein, len: 270 aa; contains probable Helix-turn-helix motif at aa 91-112, Score 1562 (+4.51 SD) and Pfam match to entry tetR PF00440, Bacterial regulatory proteins, tetR family 2 4 22 14 [Reporter]SCO5416 (9F13)_PCR/7026 [Gene]SCO5416/6404 NC_003888 SC8F4.20c, probable transcriptional regulator, len: 337 aa; previously partially sequenced therefore identical to TR:Q9X950 (EMBL:AJ237976). Similar to many proposed transcriptional regulators eg. TR:CAB61299 (EMBL:AL132991) putative AraC-family transcriptional regulator from Streptomyces coelicolor (311 aa) fasta scores; opt: 859, z-score: 986.2, E(): 0, 47.8% identity in 312 aa overlap. Contains Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family 2 4 22 13 [Reporter]SCO1923 (10B13)_PCR/7025 [Gene]SCO1923/6403 NC_003888 SCC22.05c, possible dioxygenase ferredoxin subunit, len: 105 aa; similar to many e.g. SW:BEDB_PSEPU benzene dioxygenase from Pseudomonas putida ML2 (107 aa) fasta scores; opt: 286, z-score: 390.9, E(): 1.9e-14, (40.6% identity in 101 aa overlap) 2 4 22 12 [Reporter]SCO4324 (11N9)_PCR/7024 [Gene]SCO4324/6402 NC_003888 SCD12A.07c, possible integral membrane protein, len: 92 aa. Contains possible hydrophobic membrane spanning regions. High content in alanine amino acid residues 2 3 5 14 [Reporter]SCO6894 (8B22)_PCR/4637 [Gene]SCO6894/4229 NC_003888 SC7F9.46, unknown, len: 280 aa. Contains a Pfam match to entry PF01638 DUF24, Protein of unknown function. 2 4 22 11 [Reporter]SCO4134 (12J9)_PCR/7023 [Gene]SCO4134/6401 NC_003888 SCD84.01c, possible lipoprotein, len: >160 aa; similar to N-terminal region of TR:Q9X5Q4 (EMBL:AF127374) Streptomyces lavendulae MitR, 514 aa; fasta scores: opt: 274 z-score: 307.9 E(): 1.1e-09; 34.6% identity in 133 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence,SCD72A.20, possible lipoprotein, partial CDS, len: >403aa; forms C-terminus of TR:CAB88467 (EMBL:AL353816) on the overlapping Streptomyces coelicolor cosmid. Similar to many of undefined function eg. TR:Q9X5Q4 (EMBL:AF127374) from the mitomycin C biosynthetic cluster of Streptomyces lavendulae (514 aa) fasta scores; opt: 932, z-score: 1056.7, E(): 0, 37.1% identity in 412 aa overlap. Contains Pfam match to entry PF01565 FAD_binding_4, FAD binding domain. 2 3 5 12 [Reporter]SCO1958 (10J18)_PCR/4635 [Gene]SCO1958/4228 NC_003888 SCC54.18c, uvrA, ABC excision nuclease subunit A, len: 1014aa; high level of similarity to many egs. SW:UVRA_BACSU uvrA, ABC excision nuclease subunit A from Bacillus subtilis (957 aa) fasta scores; opt: 3887, z-score: 4136.7, E(): 0, (60.8% identity in 947 aa overlap). Contains three PS00017 ATP/GTP-binding site motif A (P-loop) matches and two PS00211 ABC transporters family signatures. Also contains two Pfam match to entry PF00005 ABC_tran, ABC transporter, score 119.60, E-value 5.8e-32. 2 4 22 10 [Reporter]SCO3243 (13F9)_PCR/7022 [Gene]SCO3243/6400 NC_003888 SCE29.12c, possible myo-inositol phosphate synthase, len: 388 aa; similar to many e.g. TR:O69948 (EMBL:AL023862) from Streptomyces coelicolor (417 aa) fasta scores; opt: 955, z-score: 1034.1, E(): 0, (60.3% identity in 380 aa overlap) and SW:INO1_CITPA myo-inositol phosphate synthase from Citrus paradisi (grapefruit) fasta scores; opt: 323, z-score: 352.1, E(): 2.6e-12, (26.9% identity in 364 aa overlap). 2 3 5 11 [Reporter]SCO4367 (11F18)_PCR/4634 [Gene]SCO4367/4227 NC_003888 SCD19.22, possible oxidoreductase, len: 951 aa; N-terminal domain similar to SW:GLCD_ECOLI (EMBL:L43490) Escherichia coli glycolate oxidase subunit GlcD, 499 aa; fasta scores: opt: 351 z-score: 387.8 E(): 4.3e-14; 31.2% identity in 497 aa overlap and C-terminal domain similar to SW:GLCF_ECOLI (EMBL:L43490) Escherichia coli glycolate oxidase iron-sulfur subunit GlcF, 407 aa; blastp scores: Score= 113 (39.8 bits), Expect= 8.0e-10, Sum P(2)= 8.0e-10; Identities= 26/78 (33%), Positives= 39/78 (50%) and Identities= 61/237 (25%), Positives= 91/237 (38%). Contains Pfam match to entry PF01565 FAD_binding_4, FAD binding domain and match to Prosite entry PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature 2 3 5 10 [Reporter]SCO4284 (12B18)_PCR/4633 [Gene]SCO4284/4226 NC_003888 SCD95A.17c, probable N-acetylglucosamine-6-phosphate deacetylase, len: 381 aa; similar to SW:NAGA_BACSU (EMBL:AF017113) Bacillus subtilis N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) NagA, 396 aa; fasta scores: opt: 656 z-score: 741.7 E(): 0; 34.5% identity in 386 aa overlap 2 3 5 9 [Reporter]SCO1823 (13N14)_PCR/4632 [Gene]SCO1823/4225 NC_003888 SCI8.08c, possible integral membrane protein, len: 124 aa. Similar to the C-terminus of Streptomyces coelicolor TR:O70002 (EMBL: AL022374) hypothetical 20.6 KD protein SC5B8.16 (192 aa), fasta scores opt: 268 z-score: 343.6 E(): 9.1e-12 36.9% identity in 111 aa overlap. Contains possible membrane spanning hydrophobic domains. 2 3 5 8 [Reporter]SCO1687 (14J14)_PCR/4631 [Gene]SCO1687/4224 NC_003888 SCI30A.08, putative membrane protein, len: 130 aa; similar to SW:YIDH_ECOLI hypothetical protein from Escherichia coli (115 aa) fasta scores; opt: 291, z-score: 382.3, E(): 5.8e-14, (47.3% identity in 112 aa overlap). Contains possible hydrophobic membrane spanning regions 2 3 5 7 [Reporter]SCO0451 (15F14)_PCR/4630 [Gene]SCO0451/4223 NC_003888 SCF51A.29c, unknown, len: 284 aa. Similar to the C-terminus of many hypothetical proteins e.g. Mycobacterium tuberculosis SW:Y07S_MYCTU (EMBL; Z75555) hypothetical 69.5 KD protein CY02B10.28C (653 aa), fasta scores opt: 390 z-score: 451.1 E(): 9.1e-18 33.5% identity in 263 aa overlap. In addition to Bacillus subtilis SW:RSBU_BACSU (EMBL; L35574) sigma factor SibG regulation protein RsbU (335 aa), fasta scores opt: 206 z-score: 243.6 E(): 3.3e-06 24.3% identity in 185 aa overlap. 1 2 11 11 [Reporter]SCO4433 (11M23)_PCR/2246 [Gene]SCO4433/2049 NC_003888 SCD6.11c, possible integral membrane protein, len: 136 aa; similar to TR:O32937 (EMBL:Z98604) Mycobacterium leprae hypothetical 12.4 kD protein, 117 aa; fasta scores: opt: 308 z-score: 355.9 E(): 2.3e-12; 52.4% identity in 105 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 5 6 [Reporter]SCO3888 (16B14)_PCR/4629 [Gene]SCO3888/4222 NC_003888 StH24.10, conserved hypothetical protein, len: 205 aa; previously sequenced as TR:O07326 (EMBL:AF031590), unknown function, similar to TR:O53594 (EMBL:AL021426) Mycobacterium tuberculosis hypothetical protein (244 aa), fasta scores; opt: 392 z-score: 473.7 E(): 4.5e-19, 40.2% identity in 234 aa overlap and TR:Q50200 (EMBL:L39923) hypothetical protein (259 aa) (39.6% identity in 240 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family, score 17.80, E-value 0.018 1 2 11 10 [Reporter]SCO2832 (12I23)_PCR/2245 [Gene]SCO2832/2048 NC_003888 SCE20.06, possible IclR-family transcriptional regulator, len: 285 aa, Almost identical to a gene fragment which, like SCE20.06, is located upstream of the Streptomyces lividans lysT tRNA gene: TR:Q54411(EMBL:X52073) (200 aa), fasta scores opt: 1250 z-score: 1464.9 E():0 99.5% identity in 200 aa overlap. Also similar to Streptomyces coelicolor TR:Q9X9U3(EMBL:AL096823) putative transcriptional regulator (241 aa), fasta scores opt: 597 z-score: 704.2 E(): 7.7e-32 43.2% identity in 236 aa overlap. Contains a Pfam match to entry PF01614 IclR, Bacterial transcriptional regulator with a putative helix-turn-helix motif situated between residues 41..62 (+3.07 SD). 2 3 5 5 [Reporter]SCO3862 (17N10)_PCR/4628 [Gene]SCO3862/4221 NC_003888 SCH69.32c, putative membrane protein, len: 93 aa. Contains possible hydrophobic membrane spanning region 1 1 17 13 [Reporter]SCO2518 (10M8)_PCR/364 [Gene]SCO2518/329 NC_003888 SCC121.21c, possible two-component sensor kinase, len: 447 aa; similar to various S. coelicolor two-component sensor kinases, e.g. TR:CAB51250 (EMBL:AL096872) Streptomyces coelicolor putative two-component sensor kinase SC5F7.35c, 416 aa; fasta scores: opt: 752 z-score: 802.9 E(): 0; 40.7% identity in 410 aa overlap 1 2 11 9 [Reporter]SCO0387 (13E23)_PCR/2244 [Gene]SCO0387/2047 NC_003888 SCF62.13. possible bi-domain oxidoreductase, len: 726 aa. The N-terminus is similar to many Zinc-binding dehydrogenases e.g. Sinorhizobium meliloti TR:O31186 (EMBL; AF031940) alcohol dehydrogenase (340 aa), fasta scores pt: 224 z-score: 241.8 E(): 4.1e-06 25.5% identity in 333 aa overlap. The C-terminus is similar to other oxidoreductases e.g. Zymomonas mobilis SW:GFO_ZYMMO (EMBL; M97379) glucose-fructose oxidoreductase precursor (EC 1.1.99.28) (439 aa), fasta scores opt: 243 z-score: 260.3 E(): 3.8e-07 26.1% identity in 272 aa overlap. Note this CDS is also weakly similar to the C-terminus of the Eukaryotic: Cochliobolus heterostrophus TR:Q92217 (EMBL; U68040) polyketide synthase (2530 aa), fasta scores opt: 164 z-score:0.0 E(): 0.0 25.52% identity in 576 aa overlap. Contains a Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and a Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family. Also contains possible membrane spanning hydrophobic domains 2 3 5 4 [Reporter]SCO1707 (18J10)_PCR/4627 [Gene]SCO1707/4220 NC_003888 SCI30A.28c, probable ABC sugar transporter, ATP-binding subunit, len: 445aa; similar to many eg. TR:AAD24578 (EMBL:AF126010) trehalose/maltose transport ATP-hydrolysing subunit from hyperthermophilic archaeon Thermococcus litoralis (372 aa) fasta scores; opt: 975, z-score: 1068.2, E(): 0, (50.1% identity in 335 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite matches to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 1 1 17 12 [Reporter]SCO4393 (11I8)_PCR/363 [Gene]SCO4393/328 NC_003888 SCD10.25c, hypothetical protein, len: 251 aa; similar to SW:YFEU_ECOLI (EMBL:AE000330) Escherichia coli hypothetical 31.2 kD protein in CysP-AmiA intergenic region YfeU, 298 aa; fasta scores: opt: 147 z-score: 178.1 E(): 0.019; 27.0% identity in 185 aa overlap 1 2 11 8 [Reporter]SCO0170 (14A23)_PCR/2243 [Gene]SCO0170/2046 NC_003888 SCJ1.19c, conserved hypothetical protein, len: 223 aa. Duplication of DNA region 16907..17518. Similar to many hypothetical proteins including: Thermotoga maritima TR:AAD36216 (EMBL; AE001771) conserved hypothetical protein (215 aa), fasta scores opt: 204 z-score: 245.5 E(): 2.6e-06 29.3% identity in 133 aa overlap and S. coelicolor SCJ1.18 (217 aa), fasta scores opt: 711 z-score: 712.7 E(): 1.8e-34 56.7% identity in 215 aa overlap. Contains 2x Pfam match to entry PF00571 CBS, CBS domains 1 1 17 11 [Reporter]SCO4468 (12E8)_PCR/362 [Gene]SCO4468/327 NC_003888 SCD65.11c, unknown, len: 68 aa 1 2 11 7 [Reporter]SCO0599 (15M19)_PCR/2242 [Gene]SCO0599/2045 NC_003888 SCF55.23, probable regulator of sig8, 309 aa; identical to previously sequenced TR:CAB55832 (EMBL:AJ249580) Streptomyces coelicolor putative regulator of sig8, 309 aa and to TR:Q9ZBT2 (EMBL:AL034446) Streptomyces coelicolor putative regulatory protein SC1A9.20, 153 aa; fasta scores: opt: 167 z-score: 197.6 E(): 0.0012; 40.4% identity in 114 aa overlap 1 1 17 10 [Reporter]SCO3088 (13A8)_PCR/361 [Gene]SCO3088/326 NC_003888 SCE25.29c, doubtful CDS, function unknown, len: 81 aa. Hig content in alanine and glycine amino acid residues 1 2 11 6 [Reporter]SCO1797 (16I19)_PCR/2241 [Gene]SCO1797/2044 NC_003888 SCI5.05c, possible integral membrane protein, len: 143aa; similar to TR:P71767 (EMBL:Z79701) hypothetical protein from Mycobacterium tuberculosis (144 aa) fasta scores; opt: 344, z-score: 409.3, E(): 1.8e-15, (44.8% identity in 134 aa overlap). Contains possible membrane spanning hydrophobic regions. 1 1 17 9 [Reporter]SCO3897 (14M4)_PCR/360 [Gene]SCO3897/325 NC_003888 SCH24.19c, hypothetical protein, len: 197 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains proline/glutamine-rich region from residues 24-87 approx. and TTA (leucine) codon, possible target for bldA regulation, near N-terminus 1 2 11 5 [Reporter]SCO1084 (17E19)_PCR/2240 [Gene]SCO1084/2043 NC_003888 SCG22.30c, possible thioredoxin, len: 126 aa; similar to TR:Q50039 (EMBL:U15182) Mycobacterium leprae ThiX, 172 aa; fasta scores: opt: 253 z-score: 318.8 E(): 2.8e-10; 40.6% identity in 101 aa overlap, 172 and to SW:THIO_MYCSM (EMBL:AF023161) Mycobacterium smegmatis thioredoxin TrxA, 112 aa; fasta scores: opt: 147 z-score: 193.6 E(): 0.0026; 28.4% identity in 109 aa overlap. Contains match to Prosite entry PS00194 Thioredoxin family active site 1 1 17 8 [Reporter]SCO0821 (15I4)_PCR/359 [Gene]SCO0821/324 NC_003888 SCF43A.11c, probable threonine dehydratase, len: 325 aa; similar to many threonine dehydratases e.g. SW:THD2_ECOLI (EMBL:M21312), tdcB, Escherichia coli threonine dehydratase catabolic (329 aa), fasta scores; opt: 815 z-score: 885.6 E(): 0, 42.1% identity in 318 aa overlap. Similarity to SW:YKV8_YEAST (EMBL:X75951) Saccharomyces cerevisiae threonine dehydratase homologue (326 aa) is slightly higher (52.9% identity in 312 aa overlap). Contains Pfam match to entry PF00291 S_T_dehydratase, Pyridoxal-phosphate dependent enzymes and PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site 1 2 11 4 [Reporter]SCO1523 (18A19)_PCR/2239 [Gene]SCO1523/2042 NC_003888 SCL2.13c, conserved hypothetical protein, len: 303 aa; highly similar to TR:O59905 (EMBL:AF035619) Cercospora nicotianae singlet oxygen resistance protein, Sor1, 343 aa; fasta scores: opt: 1357 z-score: 1486.7 E(): 0; 67.9% identity in 302 aa overlap and to SW:ER1_HEVBR (EMBL:M88254) Hevea brasiliensis ethylene-inducible protein, HeveR, 309 aa; fasta scores: opt: 1281 z-score: 1404.6 E(): 0; 68.3% identity in 284 aa overlap. Contains Pfam match to entry PF01680 UPF0019, Uncharacterized protein family SNZ 1 1 17 7 [Reporter]SCO2954 (16E4)_PCR/358 [Gene]SCO2954/323 NC_003888 SCE59.13c, probable RNA polymerase sigma factor, len: 194 aa; similar to TR:CAB63191 (EMBL:AL133469) Streptomyces coelicolor putative RNA polymerase sigma factor SCM10.30, 184 aa; fasta scores: opt: 522 z-score: 641.7 E(): 2.5e-28; 54.7% identity in 161 aa overlap and to SW:SIGY_BACSU (EMBL:D83026) Bacillus subtilis RNA polymerase sigma factor SigY, 178 aa; fasta scores: opt: 211 z-score: 266.1 E(): 2.1e-07; 27.2% identity in 169 aa overlap Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) PS01063 Sigma-70 factors ECF subfamily signature 1 2 11 3 [Reporter]SCO1364 (19M15)_PCR/2238 [Gene]SCO1364/2041 NC_003888 SC10A9.06c, unknown, len: 179 aa 1 1 17 6 [Reporter]SCO1724 (17A4)_PCR/357 [Gene]SCO1724/322 NC_003888 SCI11.13, probable serine/threonine protein kinase, len: 550 aa; similar to eukaryotic-type protein kinases e.g. SW:PKAA_STRCO (EMBL:D86821), PkaA, Streptomyces coelicolor serine/threonine protein kinase (543 aa), fasta scores; opt: 628 z-score: 420.3 E(): 4.4e-16, 39.0% identity in 364 aa overlap, but divergent at C-terminus. Similar to many putative serine/threonine protein kinases e.g. TR:O83032 (EMBL:AB016932), PkaD, S.coelicolor serine/threonine protein kinase (598 aa) (49.5% identity in 444 aa overlap). Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain, PS00108 Serine/Threonine protein kinases active-site signature and PS00107 Protein kinases ATP-binding region signature 1 2 11 2 [Reporter]SCO5096 (20E15)_PCR/2237 [Gene]SCO5096/2040 NC_003888 SCBAC28G1.22c, LysR-family transcriptional regulatory protein, len: 309 aa; identical to previously sequenced TR:Q9X9U9 (EMBL:Y18817) Streptomyces coelicolor A3(2) transcriptional regulator of LysR family, 307 aa. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Also contains a possible helix-turn-helix motif at residues 22..43 (+5.22 SD) 1 1 17 4 [Reporter]SCO5332 (18I24)_PCR/355 [Gene]SCO5332/321 NC_003888 SCBAC5H2.01, unknown (fragment), len: >33 aa,SC6G9.01c, hypothetical protein, partial CDS, len: >409 aa; unknown function, possible CDS suggested by positional base preference and amino acid composition. The coding sequence has unusually low %G+C content. Alternative start codons are present at codons 9 and 27 1 1 17 3 [Reporter]SCO4025 (19E24)_PCR/354 [Gene]SCO4025/320 NC_003888 2SC10A7.29, probable tetR family transcriptional regulatory protein, len: 252 aa; similar to SW:TER4_ECOLI (EMBL:X01083) Escherichia coli tetracycline repressor protein class D TetR, 217 aa; fasta scores: opt: 193 z-score: 236.9 E(): 1.1e-05; 27.4% identity in 226 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 53..74 (+3.83 SD) 2 3 5 3 [Reporter]SCP1.28c (19F10)_PCR/4626 [Gene]SCP1.28c/4219 NC_003888 SCP1.28c, unknown, len: 117aa; 2 3 5 2 [Reporter]SCO4894 (20N6)_PCR/4625 [Gene]SCO4894/4218 NC_003888 2SCK8.20c, unknown, len: 83 aa 2 3 4 22 [Reporter]SCO4546 (1N2)_PCR/4623 [Gene]SCO4546/4217 NC_003888 2SCD4.17, possible lipoprotein, len: 216 aa; similar to TR:CAB92614 (EMBL:AL356813) Streptomyces coelicolor putative lipoprotein SCK15.23, 221 aa; fasta scores: opt: 525 z-score: 595.2 E(): 1.1e-25; 39.4% identity in 213 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal signal peptide sequence 2 3 4 21 [Reporter]SCO5260 (2J2)_PCR/4622 [Gene]SCO5260/4216 NC_003888 2SC7G11.22c, atrA, secreted protein, len: 321 aa; identical to previously sequenced TR:Q9RGW8 (EMBL:AF104994) Streptomyces coelicolor A3(2) periplasmic binding protein AtrA, 321 aa. Contains Pfam match to entry PF00497 SBP_bac_3, Bacterial extracellular solute-binding proteins, family 3 and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 2 3 4 20 [Reporter]SCO2606 (3F2)_PCR/4621 [Gene]SCO2606/4215 NC_003888 SCC88.17c, orfsyn, hypothetical protein, len: 661 aa; N-terminal region identical to previously sequenced TR:O33624 (EMBL:Y14206) Streptomyces coelicolor ORFSYN (fragment) and similar to TR:P73260 (EMBL:D90905) Synechocystis sp. hypothetical 97.4 kD protein 865 aa; fasta scores: opt: 1055 z-score: 1198.6 E(): 0; 41.2% identity in 624 aa overlap. Contains match to Prosite entry PS00133 Zinc carboxypeptidases, zinc-binding region 2 signature 2 3 4 19 [Reporter]SCO6163 (4B2)_PCR/4620 [Gene]SCO6163/4214 NC_003888 SC1A9.27c, probable sensor kinase, len: 303aa; similar to a family of sensor kinases eg. TR:O69960 (EMBL:AL022268) probable two-component sensor protein from Streptomyces coelicolor (358 aa) fasta scores; opt:1037, z-score: 1477.8, E():0, (59.2% identity in 299 aa overlap) and TR:Q52558 (EMBL:U02041) two-component sensor regulating virulence genes of Pseudomonas solanacearum (502 aa) fasta scores; opt: 388, z-score: 475.5, E():3.3e-19, (31.5% identity in 270 aa overlap). Contains possible hydrophobic membrane spanning regions 2 3 4 18 [Reporter]SCO2026 (4N22)_PCR/4619 [Gene]SCO2026/4213 NC_003888 SC3A3.04c, gltB, probable glutamate synthase large subunit, len: 1514 aa; similar to bacterial glutamate synthases e.g. TR:Q51583 (EMBL:D85230), gltB, Plectonema boryanum large subunit of NADH-dependent glutamate synthase (1530 aa), fasta scores; opt: 5662 z-score: 6172.3 E(): 0, 56.4% identity in 1518 aa overlap. Also similar to part of eukaryotic glutamate synthases e.g. SW:GLSN_MEDSA (EMBL:L01660) Medicago sativa (Alfalfa) Glutamate synthase [NADH] precursor (2194 aa) (49.9% identity in 1577 aa overlap) 1 2 10 22 [Reporter]SCO2799 (1E11)_PCR/2235 [Gene]SCO2799/2039 NC_003888 2SCC13.07c, possible secreted sugar hydrolase, len: 489 aa; similar to the C-terminal region of TR:Q9UWR7 (EMBL:AB024740) Pyrococcus kodakaraensis chitinase PK-ChiA, 1215 aa; fasta scores: opt: 671 z-score: 561.2 E(): 9.4e-24; 31.6% identity in 471 aa overlap. Contains Pfam match to entry PF00553 CBD_2, Cellulose binding domain and 3x degenerate repeat: PAPT(P/G)TP. Also possible N-terminal region signal peptide sequence 2 3 4 17 [Reporter]SCO7057 (5J22)_PCR/4618 [Gene]SCO7057/4212 NC_003888 SC4G1.23c, possible esterase, len: 322 aa; similar to TR:O33842 (EMBL:U58632) Thermotoga neapolitana acetyl xylan esterase AxeA, 325 aa; fasta scores: opt: 1173 z-score: 1264.6 E(): 0; 54.2% identity in 321 aa overlap 1 2 10 21 [Reporter]SCO1577 (2A11)_PCR/2234 [Gene]SCO1577/2038 NC_003888 SCL24.13c, argD, acetonitrile aminotransferase, len: 402 aa; highly similar to SW:ARGD_CORGL (EMBL:X86157) Corynebacterium glutamicum acetylornithine aminotransferase (EC 2.6.1.11) ArgD, 389 aa; fasta scores: opt: 1247 z-score: 1362.1 E(): 0; 52.0% identity in 379 aa overlap. Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site 2 3 4 16 [Reporter]SCO6718 (6F22)_PCR/4617 [Gene]SCO6718/4211 NC_003888 SC5F2A.01c, ddah, dimethylarginine dimethylaminohydrolase, len: 258 aa; hydrolyses asymmetrically methylated arginine with preference for dimethylated arginine over monomethylated arginine (experimental). Similar to TR:D1038106 (EMBL:AB001915) NG,NG-dimethylarginine dimethylaminohydrolase from Homo sapiens (285 aa) fasta scores; opt: 323, z-score: 384.7, E(): 4.1e-14, (35.7% identity in 272 aa overlap). 1 1 17 2 [Reporter]SCO0985 (20M20)_PCR/353 [Gene]SCO0985/319 NC_003888 SCBAC19F3.12, probable methionine synthase, len: 772aa: similar to many eg. SW:O06584 (METE_MYCTU) methionine synthase MetE from Mycobacterium tuberculosis (759 aa) fasta scores; opt: 2641, Z-score: 2854.8, 59.868% identity (60.590% ungapped) in 755 aa overlap and TR:Q9KHC5 (EMBL:AF263012) 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase-like protein from Streptomyces griseus subsp. griseus (774 aa) fasta scores; opt: 4388, Z-score: 4743.6, 85.826% identity (85.826% ungapped) in 769 aa overlap. Contains Pfam match to entry PF01717 Methionine_synt, Methionine synthase, vitamin-B12 independent. 1 2 10 20 [Reporter]SCO5736 (3M7)_PCR/2233 [Gene]SCO5736/2037 NC_003888 SC3C3.22, rpsO, 30S ribosomal protein S15, len: 95 aa; highly smiilar to many e.g. RS15_ECOLI 30S ribosomal protein S15 (88 aa), fasta scores; opt: 329 z-score: 639.0 E(): 2.5e-28, 55.7% identity in 88 aa overlap. Contains PS00362 Ribosomal protein S15 signature and Pfam match to entry PF00312 S15, Ribosomal protein S15, score 100.10, E-value 6.1e-27 2 3 4 15 [Reporter]SCO7426 (7B22)_PCR/4616 [Gene]SCO7426/4210 NC_003888 SC6D11.22c, unknown, len: 145 aa. 1 1 16 22 [Reporter]SCO1324 (1M16)_PCR/351 [Gene]SCO1324/318 NC_003888 2SCG61.06c, possible 3-ketoacyl-CoA thiolase/acetyl-CoA acetyltransferase, len: 389 aa; similar to many e.g. SW:P21151 (THIK_ECOLI) 3-ketoacyl-CoA thiolase (EC 2.3.1.16)from Escherichia coli (387 aa) fasta scores; opt: 892, z-score: 980.9, E(): 0, 42.7% identity in 403 aa overlap and SW:P54810 (THIL_PARDE) acetyl-CoA acetyltransferase (EC 2.3.1.9) from Paracoccus denitrificans (391 aa) fasta scores; opt: 892, z-score: 980.9, E(): 0, 41.7% identity in 403 aa overlap. Contains Pfam match to entry PF00108 thiolase, Thiolase and Prosite match to PS00737 Thiolases signature 2. 1 2 10 19 [Reporter]SCO2241 (4I7)_PCR/2232 [Gene]SCO2241/2036 NC_003888 SC1G2.03, probable glutamine synthetase (EC 6.3.1.2), len: 453 aa. Highly similar to many other glutamine synthetases e.g. from Clostridium acetobutylicum SW:GLNA_CLOAB(EMBL:M18966) (443 aa), fasta scores opt: 1017 z-score: 1188.9 E():0 44.4% identity in 446 aa overlap and Mycobacterium tuberculosis SW:GLN2_MYCTU(EMBL:Z70692) (446 aa), fasta scores opt: 2114 z-score: 2469.2 E():0 70.2% identity in 453 aa overlap. Contains a Pfam match to entry PF00120 gln-synt, Glutamine synthetase and a Prosite hit to PS00181 Glutamine synthetase putative ATP-binding region signature. 1 1 16 21 [Reporter]SCO2231 (2I16)_PCR/350 [Gene]SCO2231/317 NC_003888 SC10B7.26c, malE, putative maltose-binding protein, len: 423 aa; identical to previously sequenced TR:P72397 (EMBL:Y07706) Streptomyces coelicolor putative maltose-binding protein MalE, 423 aa and similar to SW:MALX_STRPN (EMBL:L08611) Streptococcus pneumoniae maltose/maltodextrin-binding protein precursor MalX, 423 aa; fasta scores: opt: 431 z-score: 470.1 E(): 8.4e-19; 28.1% identity in 434 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and two matches to Prosite entries PS00013 Prokaryotic membrane lipoprotein lipid attachment site (correctly situated) and PS01037 Bacterial extracellular solute-binding proteins, family 1 signature. Contains also possible N-terminal region peptide signal sequence 1 2 10 18 [Reporter]SCO5732 (5E7)_PCR/2231 [Gene]SCO5732/2035 NC_003888 SC3C3.18c, unknown, len: 517 aa; C-terminus is Gly-Pro-rich, and is predicted to be non-coding by FramePlot 1 1 16 20 [Reporter]SCO7631 (3E16)_PCR/349 [Gene]SCO7631/316 NC_003888 SC10F4.04c, possible secreted protein, len: 629 aa. Contains possible N-terminal region signal peptide sequence 1 2 10 17 [Reporter]SCO2048 (6A7)_PCR/2230 [Gene]SCO2048/2034 NC_003888 SC4G6.17c, hisF, cyclase, len: 251aa; highly similar to many eg. SW:HIS6_AZOBR HisF protein (cyclase) from Azospirillum brasilense (261 aa) fasta scores; opt: 1067, z-score: 1189.3, E(): 0, (64.8% identity in 250 aa overlap). Contains Pfam match to entry PF00977 His_biosynth, Histidine biosynthesis protein. 1 1 16 19 [Reporter]SCO4831 (4A16)_PCR/348 [Gene]SCO4831/315 NC_003888 SC2A6.16, probable glycine betaine ABC transport system integral membrane protein, len: 648 aa; N-terminal domain similar to SW:OPAB_BACSU (EMBL:U17292) Bacillus subtilis glycine betaine transport system permease protein OpuAb aa; fasta scores: opt: 662 z-score: 724.1 E(): 7.5e-33; 38.7% identity in 269 aa overlap and C-terminal domain similar to TR:Q9RR45 (EMBL:AF039835) Listeria monocytogenes putative glycine betaine membrane transport protein GbuB, 282 aa; fasta scores: opt: 594 z-score: 650.5 E(): 9.5e-29; 36.5% identity in 263 aa overlap. Contains 2x Pfam matches to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 1 2 10 16 [Reporter]SCO3614 (7M3)_PCR/2229 [Gene]SCO3614/2033 NC_003888 SC66T3.25c, asd2, probable aspartate-semialdehyde dehydrogenase, len: 362 aa; highly similar to many e.g. SW:DHAS_CORFL (EMBL:L16848), Asd, Corynebacterium flavum aspartate-semialdehyde dehydrogenase (344 aa), fasta scores; opt: 1018 z-score: 1179.9 E(): 0, 50.9% identity in 348 aa overlap. The start site is uncertain as the GC frame plot suggests that the start may be at codon 22. Contains Pfam match to entry PF01118 Semialdhyde_dh, Semialdehyde dehydrogenase 1 1 16 18 [Reporter]SCO6370 (5M12)_PCR/347 [Gene]SCO6370/314 NC_003888 SC4A2.06c, probable integral membrane protein, len: 344 aa; slight similarity to e.g. TR:O34524 (EMBL:AF027868) B. subtilis putative transporter yocS (321 aa), fasta scores; opt: 141 z-score: 232.8 E(): 1e-05, 23.1% identity in 324 aa overlap 1 2 10 15 [Reporter]SCO5574 (8I3)_PCR/2228 [Gene]SCO5574/2032 NC_003888 SC7A1.18c, conserved hypothetical protein, len: 141aa; similar to many eg. SW:YTFH_ECOLI from Escherichia coli (156 aa) fasta scores; opt: 396, z-score: 679.8, E(): 1.4e-30, (48.9% identity in 131 aa overlap) Also similar to SC1F2.10 (EMBL:AL031350) unknown protein from Streptomyces coelicolor (135 aa) fasta scores; opt: 305, z-score: 398.0, E(): 2.2e-17 (42.4% identity in 132 aa overlap). 1 1 16 17 [Reporter]SCO6538 (6I12)_PCR/346 [Gene]SCO6538/313 NC_003888 SC5C7.23, probable integral membrane protein, len: 141 aa. Contains possible hydrophobic membrane spanning regions 1 2 10 14 [Reporter]SCO5669 (9E3)_PCR/2227 [Gene]SCO5669/2031 NC_003888 SC8B7.09c, probable polyamine ABC-transporter integr al membrane protein, len: 309 aa; similar to many e.g. POTB _ECOLI spermidine/putrescine transport system permease (275 aa), fasta scores; opt: 548 z-score: 685.1 E(): 6.6e-31, 3 6.9% identity in 274 aa overlap. Contains Pfam match to ent ry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 38.20, E-value 1.8e -07 1 1 16 16 [Reporter]SCO3590 (7E12)_PCR/345 [Gene]SCO3590/312 NC_003888 SC66T3.01c, probable two-component system response regulator, partial CDS, len: >62 aa; similar to many e.g. SW:CUTR_STRCO (EMBL:X58793), CutR, S.coelicolor two component response regulator involved in regulation of copper metabolism (217 aa), fasta scores; opt: 231 z-score: 316.5 E(): 2.7e-10, 56.5% identity in 62 aa overlap. The CDS continues as SCH66.11c in cosmid H66 (EMBL:AL049731),SCH66.11c, probable two component response regulator, len: 213 aa; similar to many e.g. SW:CUTR_STRCO two component response regulator involved in regulation of copper uptake in Streptomyces coelicolor (217 aa) fasta scores; opt: 751, z-score: 874.8, E(): 0, (57.8% identity in 199 aa overlap). Contains Pfam matches to entry PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal and to entry PF00072 response_reg, Response regulator receiver domain. 1 2 10 13 [Reporter]SCO6490 (10A3)_PCR/2226 [Gene]SCO6490/2030 NC_003888 SC9C7.26, possible acetyltransferase, len: 199 aa; similar to SW:RIMJ_ECOLI ribosomal-protein-alanine acetyltransferase from Escherichia coli (194 aa) fasta scores; opt: 248, z-score: 351.1, E(): 2.9e-12, (38.7% identity in 111 aa overlap). 1 1 16 15 [Reporter]SCO5295 (8A12)_PCR/344 [Gene]SCO5295/311 NC_003888 SC6G9.38, hypothetical protein, len: 107 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 1 1 16 14 [Reporter]SCO6985 (9M8)_PCR/343 [Gene]SCO6985/310 NC_003888 SC8F11.11c, unknown, len: 387 aa. 2 3 4 14 [Reporter]SCO7594 (8N18)_PCR/4615 [Gene]SCO7594/4209 NC_003888 SC7H9.06, possible oxidoreductase, len: 348 aa; highly similar to TR:Q46851 (EMBL:U28377) Escherichia coli putative reductase ORF_0346, 346 aa; fasta scores: opt: 1411 z-score: 1623.6 E(): 0; 61.9% identity in 344 aa overlap, to TR:Q39151 (EMBL:L40948) Arabidopsis thaliana K+ channel protein beta subunit, 328 aa; fasta scores: opt: 639 z-score: 738.9 E(): 0; 38.9% identity in 306 aa overlap and to TR:CAB42946 (EMBL:AL049863) Streptomyces coelicolor putative ion channel subunit SC5H1.21, 319 aa; fasta scores: opt: 1347 z-score: 1354.1 E(): 0; 64.9% identity in 322 aa overlap. Contains 2x Pfam matches to entry PF00248 aldo_ket_red, Aldo/keto reductase family 2 3 4 13 [Reporter]SCO6437 (9J18)_PCR/4614 [Gene]SCO6437/4208 NC_003888 SC9B5.04, unknown, len: 424 aa; similar to two hypothetical proteins SW:Y4RH_RHISN from Rhizobium sp. fasta scores; opt: 213, z-score: 366.1, E(): 4.2e-13, (26.3% identity in 335 aa overlap) and SW:YWFE_BACSU from Bacillus subtilis (472 aa) fasta scores; opt: 217, z-score: 289.2, E(): 8.1e-09, (29.4% identity in 364 aa overlap). Also weakly similar to carbomylphosphate synthetases eg. TR:O50302 (EMBL:AJ001805) carbomylphosphate synthetase large subunit from Bacillus subtilis (1064 aa) fasta scores; opt: 150, z-score: 196.8, E(): 0.0011, (25.8% identity in 295 aa overlap). 2 3 4 12 [Reporter]SCO2459 (10F18)_PCR/4613 [Gene]SCO2459/4207 NC_003888 SCC24.30, unknown, len: 177 aa 2 3 4 11 [Reporter]SCO4363 (11B18)_PCR/4612 [Gene]SCO4363/4206 NC_003888 SCD19.18, probable two component system response regulator, len: 226 aa; similar to TR:O70001 (EMBL:AL022374) Streptomyces coelicolor putative two-component regulator SC5B8.15, 229 aa; fasta scores: opt: 691 z-score: 751.7 E(): 0; 54.4% identity in 215 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Also contains possible helix-turn-helix motif at residues 181..202 (+3.68 SD) 2 3 4 10 [Reporter]SCO3290 (12N14)_PCR/4611 [Gene]SCO3290/4205 NC_003888 SCE15.07, unknown, len: 249aa; 2 3 4 9 [Reporter]SCO3483 (13J14)_PCR/4610 [Gene]SCO3483/4204 NC_003888 SCE65.19c, probable integral membrane transport protein, len: 315 aa; similar to TR:O50452 (EMBL:AL021006) Mycobacterium tuberculosis probable sugar transport protein MTV006.08, 307 aa; fasta scores: opt: 542 z-score: 668.9 E(): 7e-30; 32.6% identity in 288 aa overlap. Contains match to Pfam entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and possible N-terminal region signal peptide sequence 2 3 4 8 [Reporter]SCO1321 (14F14)_PCR/4609 [Gene]SCO1321/4203 NC_003888 2SCG61.03c, tuf3, elongation factor TU-3, len: 392aa; previously sequenced as SW:P40175 (EFT3_STRCO). Strongly similar to many others eg. SW:O50306 (EFTU_BACST) elongation factor TU-3 from Bacillus stearothermophilus (395 aa) fasta scores; opt: 1601, z-score: 1609.5, E(): 0, 61.6% identity in 396 aa overlap and SW:P40174 (EFT1_STRCO) tuf1, elongation factor TU-1 from Streptomyces coelicolor (397 aa) fasta scores; opt: 1586, z-score: 1594.5, E(): 0, 62.7% identity in 397 aa overlap. Contains Pfam match to entry PF00009 GTP_EFTU, Elongation factor Tu family, Prosite match to PS00301 GTP-binding elongation factors signature and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 2 10 12 [Reporter]SCO2684 (10M23)_PCR/2225 [Gene]SCO2684/2029 NC_003888 SCC61A.05c, possible ATP-binding membrane protein, len: 826aa; no significant database similarities, N-terminal region rich in glycine/arginine/asparagine, C-terminal contains possible membrane-spanning hydrophobic region. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). Note possible alternative downstream translational start sites. 2 3 4 7 [Reporter]SCO3929 (15B14)_PCR/4608 [Gene]SCO3929/4202 NC_003888 SCQ11.12c, hypothetical protein, len: 450 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains TTA (leucine) codon, possible target for bldA regulation 1 2 10 11 [Reporter]SCO4429 (11I23)_PCR/2224 [Gene]SCO4429/2028 NC_003888 SCD6.07, conserved hypothetical protein, len: 867 aa; similar to TR:O50429 (EMBL:AL010186) Mycobacterium tuberculosis, 856 aa; fasta scores: opt: 3662 z-score: 4165.6 E(): 0; 62.8% identity in 845 aa overlap 2 3 4 6 [Reporter]SCO0255 (16N10)_PCR/4607 [Gene]SCO0255/4201 NC_003888 SCF20.01c, possible transcriptional regulator, len: >476aa; weakly similar to the N-terminal region of many transcriptional regulator eg. SW:ACOR_ALCEU acetoin catabolism regulatory protein from Alcaligenes eutrophus (668 aa) fasta scores; opt: 288, z-score: 292.1, E(): 6.6e-09, (26.8% identity in 496 aa overlap). Contains Pfam match to entry PF00158 sigma54, Sigma-54 transcription factors and Prosite match to PS00675 Sigma-54 interaction domain ATP-binding region A signature.,SCJ9A.34c, partial CDS, possible transcriptional regulator, len: >185aa; similar to C-terminal region of many eg. SW:HYDG_SALTY hydrogenase regulatory gene from Salmonella typhimurium (441 aa) fasta scores; opt: 207, z-score: 258.1, E(): 5.2e-07, (31.3% identity in 134 aa overlap). Contains Pfam match to entry PF00158 sigma54, Sigma-54 transcription factors. Contains possible helix-turn-helix motif (+4.35 SD) 155-176aa. 1 1 16 13 [Reporter]SCO2514 (10I8)_PCR/342 [Gene]SCO2514/309 NC_003888 SCC121.17, hypothetical protein, len: 96 aa; similar to various hypothetical proteins, e.g. TR:Q9Z544 (EMBL:AL035212) Streptomyces coelicolor hypothetical 8.2 kD protein SC9B2.15c, 77 aa; fasta scores: opt: 230 z-score: 304.4 E(): 1.5e-09; 48.1% identity in 79 aa overlap 1 2 10 10 [Reporter]SCO3394 (12E23)_PCR/2223 [Gene]SCO3394/2027 NC_003888 SCE9.01, hypothetical protein, partial CDS, len: >436 aa; unknown function, this is the remainder of CDS SCE126.12 (EMBL:AL049630). Weakly similar to TR:O33222 (EMBL:Z98260) Mycobacterium tuberculosis hypothetical protein (487 aa), fasta scores; opt: 203 z-score: 237.4 E(): 6.7e-06, 30.3% identity in 347 aa overlap. Contains hydrophobic membrane spanning region and contains probable coiled-coil from 394 to 429 (36 residues) (Max score: 1.471, probability 0.91),SCE126.12, partial CDS, unknown, proline-rich, len: >174aa; 2 3 4 5 [Reporter]SCO5155 (17J10)_PCR/4606 [Gene]SCO5155/4200 NC_003888 SCP8.18, possible lipoprotein, len: 243 aa; similar to TR:CAB76294 (EMBL:AL158057) Streptomyces coelicolor putative lipoprotein SC1G8.21c, 249 aa; fasta scores: opt: 238 z-score: 275.6 E(): 7e-08; 29.3% identity in 249 aa overlap. Contains possible N-terminal region signal peptide sequence and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site 1 1 16 12 [Reporter]SCO2547 (11E8)_PCR/341 [Gene]SCO2547/308 NC_003888 SCC77.14c, possible hydrolase, len: 301 aa. Similar to many including: Alteromonas carrageenovora SW:ARS_ALTCA (EMBL:X65709) aryl sulfatase precursor (EC 3.1.6.1) (328 aa), fasta scores opt: 196 z-score: 237.5 E(): 7.8e-06 24.9% identity in 317 aa overlap and Chlamydia pneumoniae TR:Q9Z9F6(EMBL:AE001588) sulphohydrolase/glycosulfatase (307 aa), fasta scores opt: 901 z-score: 1069.6 E():0 45.2% identity in 303 aa overlap. 1 2 10 9 [Reporter]SCO0290 (13A23)_PCR/2222 [Gene]SCO0290/2026 NC_003888 SCF85.18, probable solute-binding protein, len: 436 aa. Similar to many including: Streptomyces lividans TR:Q9Z492 (EMBL; AF043654) BxlE precursor (434 aa), fasta scores opt: 946 z-score: 1058.6 E(): 0 33.7% identity in 427 aa overlap and Streptococcus mutans SW:MSME_STRMU (EMBL; M77351) multiple sugar-binding protein precursor MsmE (420 aa), fasta scores opt: 258 z-score: 291.6 E(): 6.9e-09 24.2% identity in 351 aa overlap. Contains a Pfam match to entry PF01547 SBP_bacterial_1, PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a PS00017 ATP/GTP-binding site motif A (P-loop). Also contains a possible N-terminal signal sequence. 1 1 16 11 [Reporter]SCO4464 (12A8)_PCR/340 [Gene]SCO4464/307 NC_003888 SCD65.07c, probable hydrolase, len: 224 aa; similar to SW:ENTB_ECOLI (EMBL:M24148) Escherichia coli isochorismatase (EC 3.3.2.1) EntB, 285 aa; fasta scores: opt: 245 z-score: 306.5 E(): 1.3e-09; 29.2% identity in 202 aa overlap. Contains Pfam match to entry PF00857 Isochorismatase, Isochorismatase family and a TTA leucine codon, possible target for bldA regulation 1 2 10 8 [Reporter]SCO0845 (14M19)_PCR/2221 [Gene]SCO0845/2025 NC_003888 SCF43A.35c, unknown, len: 88 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. The N-terminus is rich in basic amino acids, while the remainder is rich in Ser/Thr 1 1 16 10 [Reporter]SCO3440 (13M4)_PCR/339 [Gene]SCO3440/306 NC_003888 SCE36.07, unknown, len: 239aa; 1 2 10 7 [Reporter]SCO0966 (15I19)_PCR/2220 [Gene]SCO0966/2024 NC_003888 SCM11.21c, probable dehydratase, len: 319 aa; similar to TR:Q9Y7K4 (EMBL:AL049495) Schizosaccharomyces pombe hypothetical 31.5 kD protein, 295 aa; fasta scores opt: 478 z-score: 525.2 E(): 7.1e-22; 40.1% identity in 312 aa overlap and to TR:O69128 (EMBL:AF064070) Burkholderia pseudomallai putative epimerase/dehydratase WbiG, 321 aa; fasta scores: opt: 231 z-score: 257.9 E(): 5.5e-07; 26.8% identity in 314 aa overlap. Contains match to Prosite entry PS00059 Zinc-containing alcohol dehydrogenases signature 1 1 16 9 [Reporter]SCO0449 (14I4)_PCR/338 [Gene]SCO0449/305 NC_003888 SCF51A.27, possible secreted solute-binding lipoprotein , len: 359 aa. Weakly similar to many solute-binding proteins e.g. Streptomyces coelicolor TR:O69944 (EMBL; AL023862) putative secreted solute binding protein SC3F9.04 (348 aa), fasta scores opt: 231 z-score: 259.4 E(): 4.3e-07 23.2% identity in 366 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to, PS00013, a prokaryotic membrane lipoprotein lipid attachment site. 1 2 10 6 [Reporter]SCO3736 (16E19)_PCR/2219 [Gene]SCO3736/2023 NC_003888 SCH22A.14c, probable RNA polymerase ECF sigma factor, len: 163 aa; similar to SW:RPOE_STRCO (EMBL:L29636) Streptomyces coelicolor RNA polymerase sigma-E factor SigE, 176 aa; fasta scores: opt: 214 z-score: 282.2 E(): 2.7e-08; 32.7% identity in 159 aa overlap 1 1 16 8 [Reporter]SCO2943 (15E4)_PCR/337 [Gene]SCO2943/304 NC_003888 SCE59.02c, probable sugar hydrolase, len: 545 aa; similar to TR:Q9ZH39 (EBML:AF072374) Pseudoalteromonas sp. S9 beta-N-acetylglucosaminidase ChiQ, 783 aa; fasta scores: opt: 1378 z-score: 1537.3 E(): 0; 41.6% identity in 512 aa overlap and to SW:HEX1_VIBFU (EMBL:U41417) Vibrio furnissii beta-hexosaminidase (EC 3.2.1.52) ExoI, 611 aa; fasta scores: opt: 1023 z-score: 1142.5 E(): 0; 40.0% identity in 487 aa overlap. Contains Pfam match to entry PF00728 Glyco_hydro_20, Glycosyl hydrolase family 20 1 2 10 5 [Reporter]SCO0644 (17A19)_PCR/2218 [Gene]SCO0644/2022 NC_003888 SCF91.04c, possible membrane protein, len: 729 aa. Contains possible membrane spanning hydrophobic domain, a possible N-terminal signal sequence and a PS00216 Sugar transport proteins signature 1. Also contains a probable coiled-coil from 76 to 107 (32 residues). 1 1 16 7 [Reporter]SCO3822 (16A4)_PCR/336 [Gene]SCO3822/303 NC_003888 SCGD3.23c, conserved hypothetical protein, len: 179 aa; unknown function, highly similar to SW:Y0GE_MYCTU (EMBL:Z80343) Mycobacterium tuberculosis hypothetical protein (178 aa), fasta scores; opt: 692 z-score: 731.2 E(): 0, 65.9% identity in 170 aa overlap 1 2 10 4 [Reporter]SCO6083 (18M15)_PCR/2217 [Gene]SCO6083/2021 NC_003888 SCBAC1A6.07, unknown, len: 415 aa 1 1 16 6 [Reporter]SCO1835 (16M24)_PCR/335 [Gene]SCO1835/302 NC_003888 SCI8.20c, possible membrane protein, len: 61 aa. Contains possible membrane spanning hydrophobic domains. 1 2 10 2 [Reporter]SCO5115 (20A15)_PCR/2215 [Gene]SCO5115/2020 NC_003888 SCBAC31E11.11, bldKD, putative ABC transporter intracellular ATPase subunit, len: 353 aa; almost identical to previously sequenced TR:P72409 (EMBL:U680360) Streptomyces coelicolor BldKD putative ABC transporter intracellular ATPase subunit, 353 aa and similar to TR:O86574 (EMBL:AL031184) Streptomyces coelicolor SC2A11.13, 366 aa; fasta scores: opt: 1427 Z-score: 1344.1 bits: 257.3 E(): 3.8e-69; 60.340% identity in 353 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 1 16 5 [Reporter]SCO3855 (17I24)_PCR/334 [Gene]SCO3855/301 NC_003888 SCH69.25c, possible membrane protein, len: 297aa; similar to TR:O53632 (EMBL:AL021926) putative transmembrane protein from Mycobacterium tuberculosis (249 aa) fasta scores; opt: 552, z-score: 598.7, E(): 5.1e-26, (45.4% identity in 251 aa overlap). Contains possible membrane spanning hydrophobic regions 1 1 16 4 [Reporter]SCO1457 (18E24)_PCR/333 [Gene]SCO1457/300 NC_003888 SCL6.14c, probable transport protein, len: 413 aa; similar to SW:MALA_BACST (EMBL:L13418) Bacillus stearothermophilus maltose permease MalA, 394 aa; fasta scores: opt: 221 z-score: 243.2 E(): 4e-06; 28.4% identity in 391 aa overlap. Contains possible hydrophobic membrane spanning regions 1 2 9 22 [Reporter]SCO2795 (1A11)_PCR/2213 [Gene]SCO2795/2019 NC_003888 2SCC13.03, probable sugar binding secreted protein, len: 441 aa; highly similar to TR:Q9X9R7 (EMBL:AJ009797) Streptomyces reticuli Cbp protein precursor (fragment) CebE, 444 aa; fasta scores: opt: 1490 z-score: 1672.5 E(): 0; 51.2% identity in 445 aa overlap and similar to SW:LACE_AGRRD (EMBL:X66596) Agrobacterium radiobacter Lactose-binding protein precursor LacE, 422 aa; fasta scores: opt: 263 z-score: 298.4 E(): 4.1e-09; 23.1% identity in 398 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 1 2 9 21 [Reporter]SCO0185 (2M7)_PCR/2212 [Gene]SCO0185/2018 NC_003888 SCJ1.34, crtB, possible geranylgeranyl pyrophosphate synthase, len: 392 aa. Shares a high level of similarity with Streptomyces griseus TR:Q54193 (EMBL:L37405) geranylgeranyl pyrophosphate synthase (425 aa), fasta scores: opt: 964 z-score: 1076.9 E(): 0 48.0% identity in 381 aa overlap and Mycobacterium tuberculosis TR:O53507 (EMBL:AL021957) geranylgeranyl pyrophosphate synthase (352 aa), fasta scores opt: 742 z-score: 831.2 E(): 0 43.0% identity in 328 aa overlap. Contains a PS00723 Polyprenyl synthetases signature 1 1 2 9 20 [Reporter]SCO4701 (3I7)_PCR/2211 [Gene]SCO4701/2017 NC_003888 SCD31.26, rpsJ, 30S ribosomal protein S10, len: 102 aa; highly similar to SW:RS10_ECOLI (EMBL:V00344) Escherichia coli 30S ribosomal protein S10 RpsJ, 103 aa; fasta scores: opt: 428 z-score: 571.1 E(): 2.2e-24; 60.8% identity in 102 aa overlap. Contains Pfam match to entry PF00338 Ribosomal_S10, Ribosomal protein S10p/S20e and match to Prosite entry PS00361 Ribosomal protein S10 signature 1 2 9 18 [Reporter]SCO5728 (5A7)_PCR/2209 [Gene]SCO5728/2016 NC_003888 SC3C3.14c, probable secreted protein, len: 212 aa; contains N-terminal signal sequence 1 2 9 17 [Reporter]SCO6667 (6M3)_PCR/2208 [Gene]SCO6667/2015 NC_003888 SC5A7.17c, probable two-component regulator, len: 222 aa; highly similar to many e.g. DEGU_BACSU transcriptional regulatory protein DegU (229 aa), fasta scores; opt: 561 z-score: 630.7 E(): 7e-28, 44.0% identity in 225 aa overlap. Contains PS00622 Bacterial regulatory proteins, luxR family signature, probable helix-turn-helix motif from aa 173 to 194 (Score 1001, +2.60 SD) and Pfam matches to entry PF00072 response_reg, Response regulator receiver domain, score 124.10, E-value 2.7e-33 and entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 86.30, E-value 6.1e-22 1 2 9 16 [Reporter]SCO3610 (7I3)_PCR/2207 [Gene]SCO3610/2014 NC_003888 SC66T3.21c, probable peptide hydrolase, len: 606 aa; similar to many peptidases e.g. (EMBL:AB01029) Sphingomonas capsulata prolyl oligopeptidase (723 aa), fasta scores; opt: 333 z-score: 359.1 E(): 1.1e-12, 25.8% identity in 620 aa overlap (dissimilar at N-terminus). Also weakly similar to eukaryotic peptidases e.g. TR:P70479 (EMBL:X14915) Rattus norvegicus acyl-peptide hydrolase (729 aa) (26.6% identity in 395 aa overlap). Contains Pfam match to entry PF00326 Prolyl_oligopep, Prolyl oligopeptidase family 1 2 9 15 [Reporter]SCO2160 (8E3)_PCR/2206 [Gene]SCO2160/2013 NC_003888 SC6G10.33, possible large membrane protein, len: 516 aa; contains possible hydrophobic membrane spanning region near the N-terminal domain 1 2 9 14 [Reporter]SCO5673 (9A3)_PCR/2205 [Gene]SCO5673/2012 NC_003888 SC8B7.05c, chiB, secreted chitinase, len: 610 aa; almost identical to S. lividans TR:D1026067 (EMBL:D84193) chitinase ChiB (EC 3.2.1.14) (610 aa), 99.3% identity in 610 aa overlap, and highly similar to many e.g. CHIX_STROI Streptomyces olivaceoviridis exochitinase 1 precursor (EC 3.2.1.14) ( 597 aa), fasta scores; opt: 3484 z-score: 2485.6 E(): 0, 86.1% identity in 596 aa overlap. Contains PS01095 Chitinases family 18 active site. Contains possible N-terminal region signal peptide sequence 1 2 9 13 [Reporter]SCO1509 (9M23)_PCR/2204 [Gene]SCO1509/2011 NC_003888 SC9C5.33c, possible hydrolase, len: 235 aa; similar to SW:YCBL_ECOLI (EMBL:AE000195) Escherichia coli hypothetical 23.8 kD protein in MukB-AspC intergenic region YcbL, 215 aa; fasta scores: opt: 284 z-score: 328.0 E(): 8.4e-11; 32.6% identity in 215 aa overlap and to TR:CAB83688 (EMBL:AL162753) Neisseria meningitidis putative hydrolase NMA0387, 210 aa; fasta scores: opt: 239 z-score: 277.9 E(): 5.2e-08; 32.5% identity in 206 aa overlap. Contains Pfam match to entry PF00753 lactamase_B, Metallo-beta-lactamase superfamily 1 2 9 12 [Reporter]SCO2768 (10I23)_PCR/2203 [Gene]SCO2768/2010 NC_003888 SCC57A.39c, unknown, len: 139 aa. 2 4 2 14 [Reporter]SCO6857 (8F17)_PCR/6586 [Gene]SCO6857/5999 NC_003888 SC7F9.09c, unknown, len: 252 aa. 2 4 2 13 [Reporter]SCO5738 (9B17)_PCR/6585 [Gene]SCO5738/5998 NC_003888 SC9A10.02, probable protease, len: 459 aa; similar to members of the insulinase family e.g. YMXG_BACSU hypothetical processing protease (409 aa), fasta scores; opt: 648 z-score: 1449.2 E(): 0, 39.7% identity in 413 aa overlap and MPP2_YEAST mitochondrial processing peptidase beta subunit (462 aa), fasta scores; opt: 583 z-score: 864.6 E(): 0, 29.7% identity in 448 aa overlap. Alternative start possible at aa 36. Contains PS00143 Insulinase family, zinc-binding region signature and Pfam match to entry PF00675 Insulinase, Insulinase (proteinase M16), score 171.30, E-value 1.6e-47 2 4 2 11 [Reporter]SCO4693 (11J13)_PCR/6583 [Gene]SCO4693/5997 NC_003888 SCD31.18, putative membrane protein, len: 86 aa. Contains possible hydrophobic membrane spanning region 2 4 2 10 [Reporter]SCO4236 (12F13)_PCR/6582 [Gene]SCO4236/5996 NC_003888 SCD8A.09, possible tRNA/rRNA methyltransferase, len: 314 aa; similar to TR:P96861 (EMBL:Z92774) Mycobacterium tuberculosis hypothetical 34.0 kD protein MTCY06G11.26c, 322 aa; fasta scores: opt: 937 z-score: 1001.6 E(): 0; 56.7% identity in 319 aa overlap and to SW:YJFH_ECOLI (EMBL:U14003) Escherichia coli hypothetical tRNA/rRNA methyltransferase YjfH (EC 2.1.1.-), 243 aa; fasta scores: opt: 519 z-score: 560.7 E(): 8.5e-24; 38.6% identity in 249 aa overlap. Contains Pfam match to entry PF00588 SpoU_methylase, SpoU rRNA Methylase family 2 4 2 9 [Reporter]SCO0578 (13B13)_PCR/6581 [Gene]SCO0578/5995 NC_003888 SCF55.02c, possible triosephosphate isomerase, len: 259 aa; similar to SW:TPIS_MYCTU (EMBL:Z95844) Mycobacterium tuberculosis triosephosphate isomerase (EC 5.3.1.1) TpiA, 261 aa; fasta scores: opt: 437 z-score: 490.8 E(): 5.8e-20; 38.9% identity in 221 aa overlap. Contains match to Pfam entry PF00121 TIM, Triosephosphate isomerase 2 4 2 8 [Reporter]SCO3689 (14N9)_PCR/6580 [Gene]SCO3689/5994 NC_003888 SCH35.35, unknown, len: 136aa; predicted based upon amino acid and codon usage. 1 2 9 11 [Reporter]SCO4676 (11E23)_PCR/2202 [Gene]SCO4676/2009 NC_003888 SCD31.01c, unknown (fragment), len: >147 aa,SCD40A.22c, unknown (fragment), len: > 176 aa 2 4 2 7 [Reporter]SCO1684 (15J9)_PCR/6579 [Gene]SCO1684/5993 NC_003888 SCI30A.05c, unknown, len: 338aa; 1 2 9 10 [Reporter]SCO3390 (12A23)_PCR/2201 [Gene]SCO3390/2008 NC_003888 SCE126.08c, possible two component sensor kinase, len: 401 aa; similar to many, often from Streptomyces coelicolor e.g. TR:O88024 (EMBL:AL031107) putative two component sensor (405 aa) fasta scores; opt: 718, z-score: 796.4, E(): 0, (42.2% identity in 313 aa overlap). Also similar to SW:DEGS_BACBR sensor protein DegS from Bacillus brevis (386 aa) fasta scores; opt: 320, z-score: 358.3, E(): 1.2e-12, (30.1% identity in 236 aa overlap). N-terminal half contains possible membrane spanning hydrophobic regions followed by a possible coiled-coil at approx. aa 180 2 4 2 6 [Reporter]SCO1907 (16F9)_PCR/6578 [Gene]SCO1907/5992 NC_003888 SCI7.25c, unknown, len: 475aa; contains Prosite match to PS00213 Lipocalin signature. Similar to TR:Q9RJ26 (EMBL:AL132644) SCI8.27C hypothetical protein from Streptomyces coelicolor (447 aa) fasta scores; opt: 380, Z-score: 401.6, E(): 9.7e-15, 32.114% identity (38.257% ungapped) in 492 aa overlap. 1 2 9 9 [Reporter]SCO1050 (13M19)_PCR/2200 [Gene]SCO1050/2007 NC_003888 SCG20A.30, possible DNA protection protein, len: 200 aa; similar to SW:DPS_ECOLI (EMBL:X69337) Escherichia coli DNA protection during starvation protein Dps, 166 aa; fasta scores: opt: 200 z-score: 257.4 E(): 7.4e-07; 27.5% identity in 138 aa overlap. Contains Pfam match to entry PF02047 DPS, Dps protein family and matches to Prosite entries PS00818 Dps protein family signature 1 and PS00819 Dps protein family signature 2 2 4 2 5 [Reporter]SCO3494 (17B9)_PCR/6577 [Gene]SCO3494/5991 NC_003888 SCE65.30c, probable sugar kinase, len: 326 aa; similar to TR:Q9ZFM1 (EMBL:AF098273) Bacillus stearothermophilus 2-keto-3-deoxy-gluconate kinase KdgK, 314 aa; fasta scores: opt: 598 z-score: 671.6 E(): 4.9e-30; 37.0% identity in 308 aa overlap and to SW:KDGK_BACSU (EMBL:L47838) Bacillus subtilis 2-dehydro-3-deoxygluconokinase (EC 2.7.1.4) KdgK, 324 aa; fasta scores: opt: 560 z-score: 629.2 E(): 1.1e-27; 35.4% identity in 311 aa overlap. Contains match to Pfam entry PF00294 pfkB, pfkB family carbohydrate kinase and match to Prosite entry PS00584 pfkB family of carbohydrate kinases signature 2 1 2 9 8 [Reporter]SCO1804 (14I19)_PCR/2199 [Gene]SCO1804/2006 NC_003888 SCI33.03, possible S-adenosylmethionine:tRNA ribosyltransferase-isomerase, len: 358 aa; similar to SW:QUEA_ECOLI (EMBL:M37702) Escherichia coli S-adenosylmethionine:tRNA ribosyltransferase-isomerase QueA, 356 aa; fasta scores: opt: 232 z-score: 260.0 E(): 6.3e-07; 27.6% identity in 373 aa overlap 2 4 2 4 [Reporter]SCO1800 (18N5)_PCR/6576 [Gene]SCO1800/5990 NC_003888 SCI5.08c, possible small secreted protein, len: 82aa; contains possible N-terminal signal sequence. 1 2 9 7 [Reporter]SCO0243 (15E19)_PCR/2198 [Gene]SCO0243/2005 NC_003888 SCJ9A.22, unknown, len: 59aa; 1 2 9 6 [Reporter]SCO0923 (16A19)_PCR/2197 [Gene]SCO0923/2004 NC_003888 SCM10.11c, probable reductase flavoprotein subunit, len: 649 aa; similar to SW:DHSA_BACSU (EMBL:M13470) Bacillus subtilis succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) SdhA, 585 aa;fasta scores: opt: 580 z-score: 658.7 E(): 2.7e-29; 36.8% identity in 609 aa overlap and to SW:FRDA_MYCTU (EMBL:Z74020) Mycobacterium tuberculosis fumarate reductase flavoprotein subunit FrdA, 583 aa; fasta scores: opt: 443 z-score: 503.1 E(): 1.2e-20; 32.2% identity in 574 aa overlap. Contains three Pfam matches to entry Pfam match to entry PF00890 FAD_binding_2, FAD binding domain 1 2 9 5 [Reporter]SCO5030 (17M15)_PCR/2196 [Gene]SCO5030/2003 NC_003888 SCK7.03, possible integral membrane protein, len: 465 aa; similar to SW:Y205_MYCTU (EMBL:AL021928) Mycobacterium tuberculosis hypothetical 38.0 kDa protein MTV033.13, 367 aa; fasta scores: opt: 892 z-score: 833.4 E(): 0; 41.1% identity in 343 aa overlap. Contains Pfam match to entry PF01594 UPF0118, Domain of unknown function DUF20 and possible hydrophobic membrane spanning regions 1 2 9 4 [Reporter]SCO4031 (18I15)_PCR/2195 [Gene]SCO4031/2002 NC_003888 2SC10A7.35c, probable integral membrane transport protein, len: 859 aa; similar to TR:Q9ZBJ1 (EMBL:AL035161) Streptomyces coelicolor putative efflux protein SC9C7.19, 815 aa; fasta scores: opt: 1355 z-score: 1335.4 E(): 0; 40.2% identity in 844 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and possible hydrophobic membrane spanning regions in N-terminal domain 1 2 9 3 [Reporter]SCO4980 (19E15)_PCR/2194 [Gene]SCO4980/2001 NC_003888 2SCK36.03c, unknown, len: 316 aa 1 2 9 2 [Reporter]SCO7484 (20M11)_PCR/2193 [Gene]SCO7484/2000 NC_003888 SCBAC17A6.17c, unknown, len: 78 aa: no significant database matches. 2 4 2 3 [Reporter]SCP1.112 (19J5)_PCR/6575 [Gene]SCP1.112/5989 NC_003888 SCP1.112, unknown, len: 256aa; 2 4 2 2 [Reporter]SCP1.133 (20B5)_PCR/6574 [Gene]SCP1.133/5988 NC_003888 SCP1.133, doubtful CDS, unknown, len: 83aa; 2 4 1 22 [Reporter]SCO5178 (1B1)_PCR/6572 [Gene]SCO5178/5987 NC_003888 2SC3B6.02, possible sulfurylase, len: 392 aa; similar to TR:AAF33130 (EMBL:AF196567) Pseudomonas stutzeri putative sulfurylase, 391 aa; fasta scores: opt: 1433 z-score: 1624.0 E(): 0; 56.2% identity in 395 aa overlap and to SW:MOEB_ECOLI (EMBL:M21151) Escherichia coli molybdopterin biosynthesis MoeB protein, 249 aa; blastp socres: Score = 526 (185.2 bits), Expect = 1.1e-50, P = 1.1e-5, Identities = 106/249 (42%), Positives = 156/249 (62%). Contains Pfam match to entry PF00899 ThiF_family, ThiF family and PF00581 Rhodanese, Rhodanese-like domain 2 4 1 21 [Reporter]SCO1756 (1N21)_PCR/6571 [Gene]SCO1756/5986 NC_003888 2SCI34.09, unknown, len: 265 aa 2 4 1 20 [Reporter]SCO6535 (2J21)_PCR/6570 [Gene]SCO6535/5985 NC_003888 SC5C7.20c, narG, probable nitrate reductase alpha chain, len: 1231 aa; highly similar to many e.g. NARG_BACSU nitrate reductase alpha chain (EC 1.7.99.4) (1228 aa), fasta scores; opt: 4306 z-score: 3216.7 E(): 0, 51.5% identity in 1235 aa overlap. Contains PS00551 and PS00490 Prokaryotic molybdopterin oxidoreductases signatures 1 and 2 and Pfam match to entry PF00384 molybdopterin, Prokaryotic molybdopterin oxidoreductases, score 79.90, E-value 1e-24 2 4 1 19 [Reporter]SCO7384 (3F21)_PCR/6569 [Gene]SCO7384/5984 NC_003888 SC10G8.11, possible transmembrane transport protein, len: 446 aa. Similar to many other putative transporters including: Streptomyces coelicolor TR:O69958(EMBL:AL022268) putative transmembrane transport protein, SC4H2.03C (411 aa), fasta scores opt: 808 z-score: 828.6 E(): 0 37.9% identity in 414 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence. 2 4 1 18 [Reporter]SCO5869 (4B21)_PCR/6568 [Gene]SCO5869/5983 NC_003888 SC2E9.10, unknown, len: 250 aa; similar to hypothetical proteins from Mycobacterium tuberculosis and M. leprae eg. TR:O07198 (EMBL:Z96072) MTCY05A6.17C (259 aa), fasta scores; opt: 213 z-score: 435.8 E(): 4.5e-17, 28.2% identity in 255 aa overlap. Contains possible coiled-coil domain from aa 1-40 2 4 1 17 [Reporter]SCO6700 (5N17)_PCR/6567 [Gene]SCO6700/5982 NC_003888 SC4C6.10c, pcaH, protocatechuate 3,4-dioxygenase beta subunit, len: 257 aa; highly similar to pcaH (EMBL:AF109386) Streptomyces sp. 2065 protocatechuate 3,4-dioxygenase beta subunit (257 aa), fasta scores; opt: 1686 z-score: 1951.2 E(): 0 91.1% identity in 257 aa overlap. Contains Pfam match to entry PF00775 Dioxygenase, Dioxygenase and PS00083 Intradiol ring-cleavage dioxygenases signature 2 4 1 16 [Reporter]SCO2174 (6J17)_PCR/6566 [Gene]SCO2174/5981 NC_003888 SC5F7.27c, possible transferase, len: 256 aa; region (120-210aa) similar to SW:UBIG_SALTY 3-demethylubiquinone-9 3-methyltransferase from Salmonella typhimurium (209 aa) fasta scores; opt: 142, z-score: 165.2, E(): 0.073, (31.6% identity in 98 aa overlap) 2 4 1 15 [Reporter]SCO2665 (7F17)_PCR/6565 [Gene]SCO2665/5980 NC_003888 SC6D10.08, unknown, len: 444 aa. Contains possible coiled-coil region 2 4 1 14 [Reporter]SCO6853 (8B17)_PCR/6564 [Gene]SCO6853/5979 NC_003888 SC7F9.05, unknown, len: 94 aa. 2 4 1 13 [Reporter]SCO5843 (9N13)_PCR/6563 [Gene]SCO5843/5978 NC_003888 SC9B10.10, unknown, len: 195 aa 2 4 1 12 [Reporter]SCO2318 (10J13)_PCR/6562 [Gene]SCO2318/5977 NC_003888 SCC53.09c, possible glycosyl transferase, len: 498 aa; similar to many e.g. TR:Q00481 (EMBL:X61917) second mannosyl transferase from Salmonella enterica (336 aa) fasta scores; opt: 325, z-score: 324.2, E(): 1.4e-10, 30.9% identity in 259 aa overlap and TR:Q9Z5B7 (EMBL:AL035478) putative transferase from Streptomyces coelicolor (406 aa) fasta scores; opt: 398, z-score: 393.8 ,E(): 1.8e-14, 32.4% identity in 454 aa overlap. Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1. 2 4 1 11 [Reporter]SCO4551 (11F13)_PCR/6561 [Gene]SCO4551/5976 NC_003888 SCD16A.32c, unknown, len: 199 aa; similar to SW:YXP2_XANCP hypothetical protein from Xanthonomas campestris (257 aa) fasta scores; opt: 119, z-score: 138.0, E(): 2.3, (31.1% identity in 167 aa overlap). 2 4 1 10 [Reporter]SCO4232 (12B13)_PCR/6560 [Gene]SCO4232/5975 NC_003888 SCD8A.05, probable transcriptional factor regulator, len: 160 aa; similar to TR:O53568 (EMBL:AL022075) Mycobacterium tuberculosis putative transcriptional regulator RV3583c, 162 aa; fasta scores: opt: 878 z-score: 1046.7 E(): 0; 85.0% identity in 160 aa overlap 2 4 1 9 [Reporter]SCO3108 (13N9)_PCR/6559 [Gene]SCO3108/5974 NC_003888 SCE41.17, conserved hypothetical protein, len: 65 aa; similar to TR:O05901 (EMBL:Z95210) Mycobacterium tuberculosis hypothetical 6.4 kDa protein MTCY21C12.03, 59 aa; fasta scores: opt: 102 z-score: 149.9 E(): 0.76; 29.3% identity in 58 aa overlap 2 4 1 8 [Reporter]SCO2980 (14J9)_PCR/6558 [Gene]SCO2980/5973 NC_003888 SCE50.08, possible integral membrane transport protein, len: 305 aa; similar to TR:Q55473 (EMBL:D64006) Synechocystis sp. membrane bound sugar transport protein, 298 aa; fasta scores: opt: 883 z-score: 1001.8 E(): 0; 44.5% identity in 272 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 2 4 1 7 [Reporter]SCO1652 (15F9)_PCR/6557 [Gene]SCO1652/5972 NC_003888 SCI41.35c, hypothetical protein, len: 374 aa; similar to various hypothetical proteins, e.g. SW:Y528_SYNY3 (EMBL:D64006) Synechocystis sp. (strain PCC 6803) hypothetical 40.5 KD protein SLL0528, 379 aa; fasta scores: opt: 547 z-score: 618.5 E(): 4.5e-27; 32.6% identity in 374 aa overlap. Contains two Pfam matches to entry PF00571 CBS, CBS domain, and a prosite match to entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature 1 3 7 12 [Reporter]SCO2426 (10A22)_PCR/4175 [Gene]SCO2426/3799 NC_003888 SCC42.07, possible regulatory protein, len: 403 aa; similar to TR:CAB88166 (EMBL:AL352956) Streptomyces coelicolor putative regulatory protein SC1H10.22c, 555 aa; fasta scores: opt: 157 z-score: 183.8 E(): 0.0091; 26.8% identity in 447 aa overlap. Contains a possible helix-turn-helix motif at residues 347..863 (+3.80 SD) and two TTA leucine codons (codon 170 and codon 174), possible targets for bldA regulation 2 4 1 6 [Reporter]SCO3760 (16B9)_PCR/6556 [Gene]SCO3760/5971 NC_003888 SCH63.07c, possible secreted protein, len: 325 aa; similar to TR:Q9X8R0 (EMBL:AL049754) Streptomyces coelicolor hypothetical 33.9 kDa protein SCH10.21c, 329 aa; fasta scores: opt: 589 z-score: 637.7 E(): 5.3e-28; 37.5% identity in 328 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 7 11 [Reporter]SCO4611 (11M18)_PCR/4174 [Gene]SCO4611/3798 NC_003888 SCD39.11, unknown, len: 197 aa 2 4 1 5 [Reporter]SCO0158 (17N5)_PCR/6555 [Gene]SCO0158/5970 NC_003888 SCJ1.07, probable oxidoreductase, len: 409 aa; similar to e.g. Synechocystis sp. (strain PCC 6803) TR:P73735 (EMBL:D90909) NADH dehydrogenase (404 aa), fasta scores opt: 358 z-score: 399.1 E(): 7.1e-15 28.9% identity in 353 aa overlap and Rhodococcus globerulus TR:Q57031(EMBL:X80041) ferredoxin reductase BPH (412 aa), fasta scores opt:294 z-score: 328.8 E(): 5.9e-11 31.3% identity in 358 aa overlap 1 3 7 10 [Reporter]SCO2916 (12I18)_PCR/4173 [Gene]SCO2916/3797 NC_003888 SCE19A.16c, conserved hypothetical protein, len: 105 aa; similar to SW:Y03G_MYCTU (EMBL:Z73902) Mycobacterium tuberculosis hypothetical protein (101 aa), fasta scores; opt: 377 z-score: 514.8 E(): 2.4e-21, 60.0% identity in 105 aa overlap and SW:Y03G_MYCLE (EMBL:U00014) Mycobacterium leprae hypothetical protein (94 aa) (70.3% identity in 74 aa overlap) 1 3 7 9 [Reporter]SCO2968 (13E18)_PCR/4172 [Gene]SCO2968/3796 NC_003888 SCE59.27c, possible cell division protein, len: 305 aa; similar to TR:P96293 (EMBL:X70031) Mycobacterium tuberculosis FtsX, 297 aa; fasta scores: opt: 591 z-score: 681.4 E(): 1.5e-30; 34.9% identity in 304 aa overlap and to SW:FTSX_ECOLI (EMBL:X04398) Escherichia coli cell division protein FtsX, 352 aa; fasta scores: opt: 194 z-score: 228.3 E(): 2.7e-05; 23.4% identity in 295 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 7 8 [Reporter]SCO0322 (14A18)_PCR/4171 [Gene]SCO0322/3795 NC_003888 SCF12.01c, possible ABC transport ATP-binding subunit, len: >230aa; similar to many eg. SW:TLRC_STRFR tylosin resistance ATP-binding protein from Streptomyces fradiae (548 aa) fasta scores; opt: 1017, z-score: 1131.8, E(): 0, (69.0% identity in 232 aa overlap). Contains Prosite match to PS00211 ABC transporters family signature and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop).,SC5G9.31c, probable ABC-transporter ATP-binding protein, partial CDS, len: >371 aa; similar ATP-binding components from antibiotic resistance export systems e.g. SW:TLRC_STRFR (EMBL:M57437), tlrC, Streptomyces fradiae tylosin resistance ATP-binding protein (548 aa), fasta scores; opt: 1517 z-score: 1597.3 E(): 0, 67.4% identity in 356 aa overlap. Also similar to many other putative ABC-transporter ATP-binding proteins. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 7 7 [Reporter]SCO3305 (15M14)_PCR/4170 [Gene]SCO3305/3794 NC_003888 SCE68.03, possible membrane protein, len: 441 aa; unknown function, weakly similar to TR:O66257 (EMBL:AB010415) Actinobacillus actinomycetemcomitans integral membrane acetyltransferase (344 aa), fasta scores; opt: 259 z-score: 247.1 E(): 1.9e-06, 24.2% identity in 360 aa overlap. Contains hydrophobic, possible membrane-spanning regions 1 3 7 6 [Reporter]SCO2615 (16I14)_PCR/4169 [Gene]SCO2615/3793 NC_003888 SCC88.26c, valS, valyl tRNA synthetase, len: 874 aa; C-terminal region identical to the previously sequenced SW:SYV_STRCO (EMBL:Y13070) Streptomyces coelicolor valyl-tRNA synhtetase (EC 6.1.1.9) ValS (fragment), 258 aa and similar to TR:Q9X2D7 (EMBL:AE001819) Thermotoga maritima valyl tRNA synthetase, 865 aa; fasta scores: opt: 2710 z-score: 3059.0 E(): 0; 47.7% identity in 874 aa overlap and to SW:SYV_BACST (EMBL:M16318) Bacillus stearothermophilus valyl-tRNA synthetase (EC 6.1.1.9) (valine-tRNA ligase) ValS, 880 aa; fasta scores: opt: 2596 z-score: 2930.0 E(): 0; 48.5% identity in 877 aa overlap. Contains Pfam match to entry PF00133 tRNA-synt_1, tRNA synthetases class I (I, L, M and V) and match to Prosite entry PS00178 Aminoacyl-transfer RNA synthetases class-I signature 1 3 7 5 [Reporter]SCO1052 (17E14)_PCR/4168 [Gene]SCO1052/3792 NC_003888 SCG20A.32c, possible regulatory protein, len: 222 aa; similar to C-terminal part of TR:CAB91114 (EMBL:AL355913) Streptomyces coelicolor putative regulatory protein SC5H4.02, 552 aa; fasta scores: opt: 195 z-score: 192.1 E(): 0.0032; 30.5% identity in 210 aa overlap. Contains possible helix-turn-helix motif at residues 172..193 (+3.29 SD) 1 3 7 4 [Reporter]SCO3163 (18A14)_PCR/4167 [Gene]SCO3163/3791 NC_003888 SCE87.14c, unknown, len: 91 aa. Similar to a hypothetical protein from Mycobacterium tuberculosis: SW:YQ32_MYCTU (EMBL:Z80225) hypothetical 10.1 KD protein (93 aa), fasta scores opt: 112 z-score: 161.8 E(): 0.12 31.3% identity in 83 aa overlap. 1 3 7 3 [Reporter]SCP1.182 (19M10)_PCR/4166 [Gene]SCP1.182/3790 NC_003888 SCP1.182, unknown, len: 251aa; 2 4 1 4 [Reporter]SCO1747 (18J5)_PCR/6554 [Gene]SCO1747/5969 NC_003888 SCI11.36c, hypothetical protein, len: 283 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Similar to TR:Q9PFB7 (EMBL:AE003917) hypothetical protein from Xylella fastidiosa (305 aa) fasta scores; opt: 394, Z-score: 443.5, E(): 4.5e-17, 35.094% identity (37.805% ungapped) in 265 aa overlap. 2 4 1 3 [Reporter]SCP1.244 (19F5)_PCR/6553 [Gene]SCP1.244/5968 NC_003888 SCP1.244, mmfH, possible oxidoreductase, len: 400aa; previously sequenced and annotated as hypothetical protein TR:Q9JN91 (EMBL:AJ276673). similar to many possible oxidoreductases eg. TR:O68921 (EMBL:AF058302) putative enoyl reductase, FrnT from polyketide antibiotic frenolicin biosynthetic cluster of Streptomyces roseofulvus (396 aa) fasta scores; opt: 867, z-score: 921.4, E(): 0, 48.0% identity in 329 aa overlap. 1 4 23 22 [Reporter]SCO1751 (1I21)_PCR/6551 [Gene]SCO1751/5967 NC_003888 2SCI34.04c, probable transmembrane transport protein, len: 534 aa; similar to TR:O33968 (EMBL:U80063) Streptomyces cinnamoneus putative transmembrane, proton-dependent transport protein, 519 aa; fasta scores: opt: 1250 z-score: 1258.8 E(): 0; 43.9% identity in 513 aa overlap and to SW:SMVA_SALTY (EMBL:D26057) Salmonella typhimurium methyl viologen resistance protein SmvA, 496 aa; fasta scores: opt: 1018 z-score: 1026.6 E(): 0; 37.4% identity in 497 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 23 21 [Reporter]SCO1658 (2E21)_PCR/6550 [Gene]SCO1658/5966 NC_003888 SCI46.03, gylR, glycerol operon regulatory protein, len: 254 aa; identical to previously sequenced SW:GYLR_STRCO (EMBL:X14188) Streptomyces coelicolor glycerol operon regulatory protein GylR, 254 aa. Contains Pfam match to entry PF01614 IclR, Bacterial transcriptional regulator and match to Prosite entry PS01051 Bacterial regulatory proteins, iclR family signature 1 4 23 20 [Reporter]SCO7379 (3A21)_PCR/6549 [Gene]SCO7379/5965 NC_003888 SC10G8.06, unknown, len: 103 aa. 1 4 23 19 [Reporter]SCO7603 (4M17)_PCR/6548 [Gene]SCO7603/5964 NC_003888 SC2H2.01c, probable lysR-family transcriptional regulator (fragment), len: >162 aa; similar to N-terminal domain of SW:TFDR_ALCEU (EMBL:M98445) Alcaligenes eutrophus transcriptional regulatory protein TdfR/TdfS, 295 aa; fasta scores: opt: 240 z-score: 294.4 E(): 7.3e-09; 37.2% identity in 145 aa overlap and of TR:CAB55524 (EMBL:AL117322) Streptomyces coelicolor putative LysR-family transcriptional regulator SCF1.04, 311 aa; fasta scores: opt: 461 z-score: 473.3 E(): 1.1e-20; 51.0% identity in 153 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Also contains possible helix-turn-helix motif at residues 24..45 (+4.95 SD),SC7H9.15c, possible regulatory protein (fragment), len: >187 aa; similar to TR:O88132 (EMBL:AJ006517) Streptomyces lipmanii lysR-type transcriptional regulator StgR, 327 aa; fasta scores: opt: 253 z-score: 307.5 E(): 1.4e-09; 30.5% identity in 187 aa overlap 1 4 23 18 [Reporter]SCO6695 (5I17)_PCR/6547 [Gene]SCO6695/5963 NC_003888 SC4C6.05c, possible membrane protein, len: 347 aa; similar to TR:O88067 (EMBL:AL031541) S.coelicolor possible membrane protein (319 aa), fasta scores; opt: 474 z-score: 403.9 E(): 3.6e-15, 37.9% identity in 354 aa overlap. Contains 4x PPXQ repeats starting at aa 46. Contains a hydrophobic, possible membrane-spanning region. Similar to TR:O88067 (EMBL:AL031541) S.coelicolor possible membrane protein (319 aa) (37.9% identity in 354 aa overlap). Contains possible hydrophobic membrane spanning region 1 4 23 17 [Reporter]SCO7790 (6E17)_PCR/6546 [Gene]SCO7790/5962 NC_003888 SC5E9.39c, possible killer toxin-like protein, len: 78 aa; similar to SKLP (Streptomyces killer toxin-like protein) from Streptomyces sp. F-287 (Ohki et al. (2001) J. Mol. Biol. 305, 109-120). No TREMBL submission. Protein isolated from culture supernatent and shown to exhibit cytocidal effects on both budding and fission yeasts. 1 3 7 2 [Reporter]SCP1.258c (20E10)_PCR/4165 [Gene]SCP1.258c/3789 NC_003888 SCP1.258c, unknown, alanine-rich, len: 166aa; 1 4 23 16 [Reporter]SCO2660 (7A17)_PCR/6545 [Gene]SCO2660/5961 NC_003888 SC6D10.03, possible sugar transport membrane protein, len: 303 aa; similar to TR:CAB59597 (EMBL:AL132662) Streptomyces coelicolor probable sugar transport inner membrane protein SCF11.20, 299 aa; fasta scores: opt: 662 z-score: 806.9 E(): 0; 38.4% identity in 297 aa overlap and to SW:YESQ_BACSU (EMBL:Z99107) Bacillus subtilis probable ABC transporter permease protein YesQ, 296 aa; fasta scores: opt: 588 z-score: 717.5 E(): 1.5e-32; 33.2% identity in 304 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Contains possible hydrophobic membrane spanning regions 1 3 6 22 [Reporter]SCO5389 (1E6)_PCR/4163 [Gene]SCO5389/3788 NC_003888 2SC6G5.33, unknown, len: 130 aa 1 4 23 15 [Reporter]SCO5964 (8M13)_PCR/6544 [Gene]SCO5964/5960 NC_003888 SC7H1.34c, probable membrane protein, len: 452 aa; contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Contains possible hydrophobic membrane spanning regions 1 3 6 21 [Reporter]SCO0788 (2A6)_PCR/4162 [Gene]SCO0788/3787 NC_003888 3SCF60.20c, unknown, len: 78 aa 1 3 6 20 [Reporter]SCO1223 (3M2)_PCR/4161 [Gene]SCO1223/3786 NC_003888 2SCG58.23, rocD, ornithine aminotransferase, len: 407 aa; highly similar to SW:OAT_BACSU (EMBL:X81802) Bacillus subtilis ornithine aminotransferase (EC 2.6.1.13) RocD, 401 aa; fasta scores: opt: 1356 z-score: 1462.1 E(): 0; 50.4% identity in 395 aa overlap. Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate and match to Prosite entry PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site 1 3 6 19 [Reporter]SCO6040 (4I2)_PCR/4160 [Gene]SCO6040/3785 NC_003888 SC1C3.28c, probable lipoprotein, len: 327 aa; contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains possible hydrophobic membrane spanning region at C-terminal domain 1 3 6 18 [Reporter]SCO6340 (5E2)_PCR/4159 [Gene]SCO6340/3784 NC_003888 SC3A7.08, unknown, len: 211 aa; similar to hypothetical proteins e.g. S. coelicolor TR:O54167 (EMBL:AL021411) SC7H1.08C (202 aa), fasta scores; opt: 243 z-score: 810.6 E(): 0, 43.8% identity in 210 aa overlap 1 3 6 17 [Reporter]SCO7344 (6A2)_PCR/4158 [Gene]SCO7344/3783 NC_003888 SC4G10.23c, possible secreted protein, len: 258 aa. Weakly similar to the Eukaryotic protein elicitor from Pythium aphanidermatum TR:Q9SPD4(EMBL:AF179598) 25 kda protein elicitor (234 aa), fasta scores opt: 279 z-score: 316.6 E(): 3.6e-10 31.0% identity in 242 aa overlap. Contains a possible N-terminal signal sequence 1 3 6 16 [Reporter]SCO4837 (6M22)_PCR/4157 [Gene]SCO4837/3782 NC_003888 SC5G8.05, glyA1, serine hydroxymethyltransferase, len: 481 aa; strongly similar to many e.g. SW:P34895 (GLYA_HYPME) serine hydroxymethyltransferase from Hyphomicrobium methylovorum (434 aa) fasta scores; opt: 912, z-score: 1044.8, E(): 0, 44.2% identity in 459 aa overlap. Contains two Pfam matches to entry PF00464 SHMT, Serine hydroxymethyltransferase. 1 3 6 15 [Reporter]SCO6960 (7I22)_PCR/4156 [Gene]SCO6960/3781 NC_003888 SC6F7.13c, unknown, len: 178 aa. 1 3 6 14 [Reporter]SCO5934 (8E22)_PCR/4155 [Gene]SCO5934/3780 NC_003888 SC7H1.04, possible sigma factor, len: 200 aa; some similarity to sigma factors (ECF subfamily) from diverse organisms e.g. Alcaligenes eutrophus CNRH_ALCEU P37978 rna polymerase sigma factor cnrh (191 aa), fasta scores; opt: 230 z-score: 337.3 E(): 1.4e-11, 29.5% identity in 183 aa overlap 1 4 23 14 [Reporter]SCO5837 (9I13)_PCR/6543 [Gene]SCO5837/5959 NC_003888 SC9B10.04, probable zinc protease, len: 450 aa; weakly similar to many of prokaryotic and eukaryotic origin eg. Y4WA_RHISN P55679 hypothetical zinc protease y4wa from Rhizobium sp. (512 aa), fasta scores; opt: 617 z-score: 325.0 E(): 6.1e-11, 30.8% identity in 426 aa overlap; and MPP2_YEAST P10507 mitochondrial processing peptidase beta subunit (462 aa), fasta scores; opt: 355 z-score: 321.6 E(): 9.4e-11, 25.3% identity in 415 aa overlap. The zinc-binding and active site residues are conserved 1 4 23 13 [Reporter]SCO1926 (10E13)_PCR/6542 [Gene]SCO1926/5958 NC_003888 SCC22.08c, possible DNA-binding protein, len: 252aa; similar to TR:O53151 (EMBL:AL021184) hypothetical protein from Mycobacterium tuberculosis (278 aa) fasta scores; opt: 629, z-score: 705.6, E(): 5.8e-32, (46.8% identity in 233 aa overlap). Contains a helix-turn-helix motif (+4.75 SD) at residues 34-55 1 4 23 12 [Reporter]SCO4556 (11A13)_PCR/6541 [Gene]SCO4556/5957 NC_003888 SCD16A.27c, possible ubiquinone/menaquinone methyltransferase, len: 231aa; similar to many egs. TR:O06424 (EMBL:Z95558) hypothetical protein from Mycobacterium tuberculosis (234 aa) fasta scores; opt: 1009, z-score: 1223.5, E(): 0, (67.7% identity in 229 aa overlap) and SW:UBIE_ECOLI ubiquinone/menaquinone methyltransferase from Escherichia coli (251 aa) fasta scores; opt: 456, z-score: 557.5, E(): 1e-23, (42.7% identity in 227 aa overlap). Contains Pfam match to entry PF01209 Ubie_methyltran, ubiE/COQ5 methyltransferase family. 1 4 23 11 [Reporter]SCO4137 (12M9)_PCR/6540 [Gene]SCO4137/5956 NC_003888 SCD84.04, hypothetical protein, len: 206 aa; similar to TR:AAF10501 (EMBL:AE001945) Deinococcus radiodurans conserved hypothetical protein DR0924, 212 aa; fasta scores: opt: 347 z-score: 407.5 E(): 3.1e-15; 32.4% identity in 210 aa overlap. Contains Pfam match to entry PF01865 DUF47, Protein of unknown function 1 4 23 10 [Reporter]SCO3103 (13I9)_PCR/6539 [Gene]SCO3103/5955 NC_003888 SCE41.12c, possible integral membrane protein, len: 201 aa. Contains possible hydrophobic membrane spanning regions 1 4 23 9 [Reporter]SCO3926 (14E9)_PCR/6538 [Gene]SCO3926/5954 NC_003888 SCQ11.09, ssgA, probable regulator, len: 136 aa; highly similar to TR:P95753 (EMBL:D50051), SsgA, Streptomyces griseus protein associated with sporulation and cell division (136 aa), fasta scores; opt: 715 z-score: 896.6 E(): 0, 77.9% identity in 136 aa overlap and similar to others from S.coelicolor e.g. SC5F2A.05c (EMBL:AL049587) possible regulator (138 aa) (35.7% identity in 115 aa overlap) and SC5H1.03 (EMBL:AL049863) possible regulator (142 aa) (2.5% identity in 114 aa overlap), both annotated by similarity to Streptomyces griseus SsgA. The start codon was selected by similarity to ssgA 1 4 23 8 [Reporter]SCO3587 (15A9)_PCR/6537 [Gene]SCO3587/5953 NC_003888 SCH66.08c, possible regulatory protein, len: 197 aa; similar to many e.g. TR:CAB37593 (EMBL:AL035569) putative transcriptional regulator from Streptomyces coelicolor (196 aa) fasta scores; opt: 386, z-score: 483.1, E(): 1.4e-19, (35.5% identity in 169 aa overlap) and SW:MTRR_NEIGO MtrR regulatory protein from Neisseria gonorrhoeae (210 aa) fasta scores; opt: 109, z-score: 143.8, E(): 1.1, (23.3% identity in 176 aa overlap). Contains possible helix-turn-helix (+2.97 SD) 26-47 aa. 1 4 23 7 [Reporter]SCO1904 (16M5)_PCR/6536 [Gene]SCO1904/5952 NC_003888 SCI7.22c, probable transcriptional regulator, len: 202 aa; similar to many e.g. TR:CAA22690 (EMBL:AL035159) putative transcriptional regulator from Mycobacterium leprae (189 aa) fasta scores; opt: 353, z-score: 418.1, E(): 5.9e-16, (38.6% identity in 189 aa overlap) and TR:O24739 (EMBL:AB001609) butyrolactone synthesis transcriptional regulator in Streptomyces virginiae (216 aa) fasta scores; opt: 191, z-score: 231.1, E(): 1.5e-05, (35.0% identity in 163 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Also contains a helix-turn-helix motif (+5.38 SD) 46-67aa. 1 3 6 13 [Reporter]SCO6055 (9A22)_PCR/4154 [Gene]SCO6055/3779 NC_003888 SC9B1.02c, probable carbonic anhydrase, len: 193aa; similar to many eg. SW:CYNT_ECOLI carbonic anhydrase from Escherichia coli (219 aa) fasta scores; opt: 512, z-score: 618.8, E(): 3.8e-27, (43.6% identity in 188 aa overlap). Contains two Pfam matches to entry PF00484 Pro_CA, Prokaryotic-type carbonic anhydrases and Prosite matches to PS00705 Prokaryotic-type carbonic anhydrases signature 2 and PS00704 Prokaryotic-type carbonic anhydrases signature 1. 1 4 23 6 [Reporter]SCO2960 (17I5)_PCR/6535 [Gene]SCO2960/5951 NC_003888 SCE59.19c, possible integral membrane protein, len: 315 aa. Contains possible hydrophobic membrane spanning regions and proline rich C-terminal region 1 3 6 12 [Reporter]SCO1961 (10M18)_PCR/4153 [Gene]SCO1961/3778 NC_003888 SCC54.21c, aroQ, dehydroquinate dehydratase, len: 157aa; identical to (except for disagreement on start codon, M or V) TR:O33608 (EMBL:AJ001493) aroQ, dehydroquinate dehydratase from Streptomyces coelicolor (157 aa) fasta scores; opt: 1049, z-score: 1325.4, E(): 0, (99.4% identity in 157 aa overlap). Contains PS01029 Dehydroquinase class II signature and Pfam match to entry PF01220 DHquinase_II, Dehydroquinase class II , score 234.80, E-value 1.2e-66. 1 4 23 5 [Reporter]SCO3684 (18E5)_PCR/6534 [Gene]SCO3684/5950 NC_003888 SCH35.40, unknown, len: 196aa; similar to neighbouring gene SCH35.39c. 1 3 6 11 [Reporter]SCO4529 (11I18)_PCR/4152 [Gene]SCO4529/3777 NC_003888 SCD35.36, possible integral membrane protein, len: 117 aa. Contains possible hydrophobic membrane spanning regions 1 3 6 10 [Reporter]SCO4287 (12E18)_PCR/4151 [Gene]SCO4287/3776 NC_003888 SCD95A.20, hypothetical protein, len: 84 aa; similar to TR:P96276 (EMBL:Z84724) Mycobacterium tuberculosis hypothetical 11.7 kD protein MTCY22G10.27, 102 aa; fasta scores: opt: 244 z-score: 340.2 E(): 1.7e-11; 54.2% identity in 72 aa overlap 1 3 6 9 [Reporter]SCO0153 (13A18)_PCR/4150 [Gene]SCO0153/3775 NC_003888 SCJ1.02c, probable secreted protein, len: 195 aa, similar to Actinosynnema pretiosum auranticum hypothetical 20.0 KD protein TR:O69206 (EMBL:U33059) (190 aa), fasta scores, opt: 524 z-score: 529.6 E(): 3.9e-22; 43.2% identity in 192 aa overlap. Contains a possible N-terminal signal peptide sequence 1 3 6 8 [Reporter]SCO0859 (14M14)_PCR/4149 [Gene]SCO0859/3774 NC_003888 SCM2.12c, possible membrane protein, len: 169 aa. . Contains possible hydrophobic membrane spanning region 1 3 6 7 [Reporter]SCO3718 (15I14)_PCR/4148 [Gene]SCO3718/3773 NC_003888 SCH35.06, probable cation transport system component, len: 554 aa; similar to many e.g. SW:ATKA_ECOLI AtkA/KdpA potassium transporting ATPase A chain from Escherichia coli (557 aa) fasta scores; opt: 1619, z-score: 1774.2, E(): 0, (48.3% identity in 555 aa overlap). Contains possible membrane spanning hydrophobic regions 4 1 11 13 [Reporter]SCO5129 (10H4)_PCR/1744 [Gene]SCO5129/1599 NC_003888 SC9E12.14, possible ABC transporter ATP-binding protein, len: 318 aa; similar to TR:CAB88183 (EMBL:AL352972) Streptomyces coelicolor putative ABC transporter ATP-binding protein SCC30.13, 308 aa; fasta scores: opt: 861 z-score: 926.3 E(): 0; 54.4% identity in 309 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 3 6 6 [Reporter]SCO0201 (16E14)_PCR/4147 [Gene]SCO0201/3772 NC_003888 SCJ12.13c, possible integral membrane protein, len: 223 aa. Contains potential membrane spanning hydrophobic domains. 4 1 11 12 [Reporter]SCO2270 (11D4)_PCR/1743 [Gene]SCO2270/1598 NC_003888 SCC75A.16c, possible membrane protein, len: 539 aa. Contains a possible N-terminal signal sequence, a possible membrane spanning hydrophobic domain and is rich in amino acids Ala, Gly and Thr. 1 3 6 5 [Reporter]SCO3863 (17A14)_PCR/4146 [Gene]SCO3863/3771 NC_003888 SCH69.33, putative secreted protein, len: 129 aa; similar to TR:CAA09663 (EMBL:AJ011500) hypothetical protein from the granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22 (124 aa) fasta scores; opt: 259, z-score: 293.5, E(): 5.1e-09, (41.5% identity in 118 aa overlap). Contains possible N-terminal region signal peptide sequence 4 1 11 11 [Reporter]SCO4444 (11P24)_PCR/1742 [Gene]SCO4444/1597 NC_003888 SCD6.22c, probable glutathione peroxidase, len: 193 aa; similar to SW:GPO_LACLC (EMBL:AJ000109) Lactococcus lactis glutathione peroxidase (EC 1.11.1.9) Gpo, 157 aa; fasta scores: opt: 530 z-score: 660.6 E(): 2.4e-29; 54.9% identity in 142 aa overlap. Contains Pfam match to entry PF00255 GSHPx, Glutathione peroxidases and match to Prosite entry PS00460 Glutathione peroxidases selenocysteine active site 1 3 6 4 [Reporter]SCO1737 (18M10)_PCR/4145 [Gene]SCO1737/3770 NC_003888 SCI11.26, hypothetical protein, len: 124 aa; unknown function, similar to TR:O86372 (EMBL:AL022003) Mycobacterium tuberculosis hypothetical protein (116 aa), fasta scores; opt: 129 z-score: 177.0 E(): 0.016, 34.7% identity in 98 aa overlap 4 1 11 9 [Reporter]SCO5039 (13H24)_PCR/1740 [Gene]SCO5039/1596 NC_003888 SCK7.12, possible penicillin-binding protein, len: 773 aa; similar to TR:CAB88838 (EMBL:AL353832) Streptomyces coelicolor probable penicillin-binding protein SCE6.34, 756 aa; fasta scores: opt: 1080 z-score: 911.6 E(): 0; 46.0% identity in 733 aa overlap and to SW:PBPD_BACSU (EMBL:U11882) Bacillus subtilis penicillin-binding protein 4 precursor PbpD, 624 aa; fasta scores: opt: 513 z-score: 438.2 E(): 6.1e-17; 28.7% identity in 582 aa overlap. Contains Pfam matches to entries PF00912 Transglycosyl, Transglycosylase and PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and 2x matches to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 1 11 8 [Reporter]SCO5049 (14D24)_PCR/1739 [Gene]SCO5049/1595 NC_003888 SCK7.22, hypothetical protein, len: 196 aa; similar to TR:P75894 (EMBL:AE000202) Escherichia coli hypothetical protein, 196 aa; fasta scores: opt: 466 z-score: 572.4 E(): 2e-24; 40.6% identity in 192 aa overlap and to SW:YDFN_BACSU (EMBL:AB001488) Bacillus subtilis putative NAD(P)H nitroreductase YdfN, 206 aa; fasta scores: opt: 133 z-score: 170.9 E(): 0.047; 23.1% identity in 199 aa overlap. Contains Pfam match to entry PF00881 Nitroreductase, Nitroreductase family. Similar also to TR:Q9FBV0 (EMBL:AL391072) Streptomyces coelicolor putative nitroreductase SC9A4.03c, 202 aa; fasta scores: opt: 599 Z-score: 706.0 E(): 1.1e-31; 49.474% identity in 190 aa overlap 4 1 11 7 [Reporter]SCO3915 (15P20)_PCR/1738 [Gene]SCO3915/1594 NC_003888 SCH24.37, probable transmembrane efflux protein, len: 404 aa; similar to many e.g. TR:Q56147 (EMBL:U09991), cmlV, Streptomyces violaceus chloramphenicol efflux pump (436 aa), fasta scores; opt: 1012 z-score: 1048.2 E(): 0, 40.5% identity in 395 aa overlap. Similar to other putative efflux proteins from S.coelicolor e.g. SCE94.17c (EMBL:AL049628) possible exporter (531 aa) (26.8% identity in 395 aa overlap). Contains several hydrophobic, probable membrane-spanning regions. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter, score -61.60, E-value 0.00088 4 1 11 6 [Reporter]SCO3827 (16L20)_PCR/1737 [Gene]SCO3827/1593 NC_003888 SCGD3.28c, probable molybdenum cofactor biosynthesis protein, len: 465 aa; similar to many e.g. SW:MOEA_ECOLI (EMBL:M21151), moeA, Escherichia coli molybdenum cofactor biosynthesis protein (411 aa), fasta scores; opt: 481 z-score: 484.8 E(): 1.1e-19, 32.9% identity in 331 aa overlap. This sequence is some 50 aa longer than many other predicted moeA homologues. The position of the start codon is suggested by the GC frameplot in this region. Contains Pfam match to entry PF00994 MoCF_biosynth, Molybdenum cofactor biosynthesis protein 4 1 11 5 [Reporter]SCO3168 (17H20)_PCR/1736 [Gene]SCO3168/1592 NC_003888 SCE87.19, possible protease, len: 1171 aa. Similar to Thermoplasma acidophilum TR:P96086(EMBL:U72850) tricorn protease (1071 aa) opt: 829 z-score: 857.6 E(): 0 28.1% identity in 1158 aa overlap and a protein of unknown function from Sulfolobus solfataricus TR:P95871(EMBL: Y08256) ORF C06024 (1068 aa), fasta scores opt: 846 z-score: 875.3 E(): 0 30.3% identity in 1150 aa overlap. 4 1 11 4 [Reporter]SCO3492 (18D20)_PCR/1735 [Gene]SCO3492/1591 NC_003888 SCE65.28c, unknown, len: 42 aa 4 1 11 3 [Reporter]SCP1.157 (19P16)_PCR/1734 [Gene]SCP1.157/1590 NC_003888 SCP1.157, unknown, len: 128aa; 1 4 23 4 [Reporter]SCP1.310c (19A5)_PCR/6533 [Gene]SCP1.310c/5949 NC_003888 SCP1.310c, possible oxidoreductase, len: 338aa; similar to many eg. TR:Q9RJW9 (EMBL:AL133210) putative reductase (fragment) from Streptomyces coelicolor (311 aa) fasta scores; opt: 795, z-score: 852.7, E(): 0, 44.0% identity in 293 aa overlap and SW:O82864 (AHPF_PSEPU) alkyl hydroperoxide reductase subunit from Pseudomonas putida (520 aa) fasta scores; opt: 301, z-score: 325.3, E(): 1.2e-10, 25.6% identity in 301 aa overlap. 1 4 23 3 [Reporter]SCO5356 (20M1)_PCR/6532 [Gene]SCO5356/5948 NC_003888 SCBAC5H2.25, thrB, homoserine kinase, len: 309 aa; similar to SW:KHSE_CORGL (EMBL:Y00546) Corynebacterium glutamicum homoserine kinase (EC 2.7.1.39) ThrB, 308 aa; fasta scores: opt: 728 z-score: 820.7 E(): 0; 43.3% identity in 275 aa overlap. Contains Pfam match to entry PF00288 GHMP_kinases, GHMP kinases putative ATP-binding proteins and match to Prosite entry PS00627 GHMP kinases putative ATP-binding domain 1 4 22 22 [Reporter]SCO1139 (1E21)_PCR/6530 [Gene]SCO1139/5947 NC_003888 2SCG38.32, possible integral membrane protein, len: 181 aa. Contains possible hydrophobic membrane spanning regions 1 4 22 21 [Reporter]SCO0656 (2A21)_PCR/6529 [Gene]SCO0656/5946 NC_003888 SCF91.16, gvpL2, possible gas vesicle synthesis protein, len: 280 aa. Weakly similar to Bacillus megaterium TR:O6872 (EMBL:AF053765) gas vesicle protein GvpL (269 aa), fasta scores opt: 172 z-score: 189.1 E(): 0.0037 19.8% identity in 222 aa overlap. Also similar to Streptomyces coelicolor TR:Q9ZC07 (EMBL:AL033505) putative gas vesicle synthesis protein GvpL (259 aa), fasta scores opt: 436 z-score: 462.0 E(): 2.3e-18 37.0% identity in 254 aa overlap. Contains possible N-terminal region signal peptide sequence 1 4 22 20 [Reporter]SCO6415 (3M17)_PCR/6528 [Gene]SCO6415/5945 NC_003888 SC1A6.04, probable dihydropyrimidinase, len: 467 aa; similar to many e.g.TR:Q45515 (EMBL:S73773) hydantoinase from Bacillus stearothermophilus (471 aa), fasta scores; opt: 1585 z-score: 1511.4 E(): 0, 50.9% identity in 458 aa overlap and to SW:HYDA_PSEPU (EMBL:U84197) Pseudomonas putida D-hydantoinase (EC 3.5.2.2) Dht, 495 aa; fasta scores: opt: 1213 Z-score: 1348.8; 41.722% identity in 453 aa overlap 1 4 22 19 [Reporter]SCO7230 (4I17)_PCR/6527 [Gene]SCO7230/5944 NC_003888 SC2H12.29, possible two-component system response regulator, len: 229 aa. Highly similar to many other putative two-component system regulators e.g. Mycobacterium bovis SW:RGX3_MYCBO (EMBL:Y13627) sensory transduction protein, RegX3 (227 aa), fasta scores opt: 609 z-score: 727.0 E(): 0 44.7% identity in 226 aa overlap and Streptomyces coelicolor TR:CAB90891 (EMBL:AL355753) two-component system response regulator protein, SC8G12.09 (233 aa), fasta scores opt: 953 z-score: 1131.9 E(): 0 63.4% identity in 235 aa overlap. Contains a Pfam match to entry PF00072 response_reg, Response regulator receiver domain and a Pfam match to entry PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal. 1 4 22 18 [Reporter]SCO2407 (5E17)_PCR/6526 [Gene]SCO2407/5943 NC_003888 SC4A7.35, probable aldose 1-epimerase, len: 366 aa; similar to SW:GALM_ACICA (EMBL:X03893) Acinetobacter calcoaceticus aldose 1-epimerase precursor (EC 5.1.3.3) Mro, 381 aa; fasta scores: opt: 1022 z-score: 1184.4 E(): 0; 44.3% identity in 350 aa overlap and to S. coelicolor SC4A7.21, 323 aa; fasta scores: opt: 838 z-score: 928.5 E(): 0; 54.2% identity in 347 aa overlap. Contains Pfam match to entry PF01263 Aldose_epim, Aldose 1-epimerase 1 4 22 17 [Reporter]SCO7787 (6A17)_PCR/6525 [Gene]SCO7787/5942 NC_003888 SC5E9.35c, unknown, len: 122 aa 1 3 6 3 [Reporter]SCP1.105 (19I10)_PCR/4144 [Gene]SCP1.105/3769 NC_003888 SCP1.105, possible integral membrane plasmid transfer protein, len: 153aa; similar to TR:Q57241 (EMBL:U23762) SpbB3 protein (involved in intramycelial plasmid spread) from plasmid pJV1 of Streptomyces phaeochromogenes (147 aa) fasta scores; opt: 317, z-score: 394.0, E(): 1.8e-14, 46.5% identity in 142 aa overlap. 1 4 22 16 [Reporter]SCO1422 (7M13)_PCR/6524 [Gene]SCO1422/5941 NC_003888 SC6D7.17, possible membrane protein, len: 194 aa. Contains possible membrane spanning hydrophobic domains. 1 3 6 2 [Reporter]SCP1.278 (20A10)_PCR/4143 [Gene]SCP1.278/3768 NC_003888 SCP1.278, unknown, len: 361aa; 1 4 22 15 [Reporter]SCO5955 (8I13)_PCR/6523 [Gene]SCO5955/5940 NC_003888 SC7H1.25c, putative membrane protein, len: 144 aa; similar to Mycobacterium tuberculosis hypothetical protein O06193 (EMBL:Z95387) MTCY1A10.13 (141 aa), fasta scores; opt: 459 z-score: 576.2 E(): 6.7e-25, 56.4% identity in 140 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 5 22 [Reporter]SCO5385 (1A6)_PCR/4141 [Gene]SCO5385/3767 NC_003888 2SC6G5.29, possible 3-hydroxybutyryl-coA dehydrogenase, len: 282 aa; similar to many eg. SW:P52041 (HBD_CLOAB) 3-hydroxybutyryl-coA dehydrogenase from Clostridium acetobutylicum (282 aa) fasta scores; opt: 806, z-score: 947.0, E(): 0, 44.6% identity in 280 aa overlap. Contains Pfam match to entry PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase and Prosite match to PS00067 3-hydroxyacyl-CoA dehydrogenase signature. 1 3 5 21 [Reporter]SCO2291 (2M2)_PCR/4140 [Gene]SCO2291/3766 NC_003888 SCC75A.37, axeA, secreted acetylxylan esterase, len: 335 aa. Almost identical in sequence with Streptomyces lividans TR:Q54413 (EMBL:M64552) acetylxylan esterase precursor (EC 3.1.1.72) (335 aa), fasta scores opt: 2197 z-score: 2058.4 E(): 0 98.5% identity in 335 aa overlap. Also highly similar to the C-terminus of Cellulomonas fimi SW:XYND_CELFI (EMBL:X76729) endo-1,4-beta-xylanase D precursor (EC 3.2.1.8) (644 aa), fasta scores opt: 1108 z-score: 1040.5 E(): 0 50.0% identity in 346 aa overlap. Also similar to the adjoining CDS on this cosmid, xlnB, (335 aa), fasta scores opt: 652 z-score: 554.2 E(): 1.7e-25 56.2% identity in 201 aa overlap. Contains a possible N-terminal signal sequence and a Pfam match to entry PF01522 Polysac_deacet, Polysaccharide deacetylase 1 3 5 20 [Reporter]SCO2688 (3I2)_PCR/4139 [Gene]SCO2688/3765 NC_003888 SCC61A.09, ribD, riboflavin-specific deaminase, len: 381aa; similar to many eg. SW:P25539 (RIBD_ECOLI) ribD, riboflavin-specific deaminase from Escherichia coli (367 aa) fasta scores; opt: 581, z-score: 642.9, E(): 2.4e-28, 36.3% identity in 386 aa overlap. Contains Pfam match to entry PF01872 RibD_C, RibD C-terminal domain, Pfam match to entry PF00383 dCMP_cyt_deam, Cytidine and deoxycytidylate deaminase zinc-binding region and PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. 1 3 5 19 [Reporter]SCO6896 (4E2)_PCR/4138 [Gene]SCO6896/3764 NC_003888 SC1B2.02, possible integral membrane protein, len: 411 aa. Weakly similar to several e.g. Escherichia coli TR:Q47390(EMBL:D16251) erythromycin resistance determinant, MrX (388 aa), fasta scores opt: 263 z-score: 283.2 E(): 2.6e-08 26.5% identity in 389 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 1 3 5 18 [Reporter]SCO6334 (5A2)_PCR/4137 [Gene]SCO6334/3763 NC_003888 SC3A7.02c, probable transcritional regulator, len: 892 aa; similar to e.g. MALT_ECOLI MalT regulatory protein (901 aa), fasta scores; opt: 540 z-score: 495.9 E(): 2.3e-20, 26.2% identity in 936 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop), helix-turn-helix motif from aa 848 to 869 (Score 1229, +3.37 SD) and Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 50.40, E-value 3.8e-11 4 1 11 2 [Reporter]SCO3993 (20H16)_PCR/1733 [Gene]SCO3993/1589 NC_003888 SCBAC25E3.30c, unknown, doubtful CDS, len: 89 aa: no significant database matches. 1 3 5 17 [Reporter]SCO7060 (5M22)_PCR/4136 [Gene]SCO7060/3762 NC_003888 SC4G1.26c, conserved hypothetical protein, len: 201 aa; similar to TR:Q9S2N1 (EMBL:AL109661) Streptomyces coelicolor hypothetical 24.3 kDa protein SC6E10.06c, 215 aa; fasta scores: opt: 540 z-score: 638.3 E(): 4.4e-28; 42.9% identity in 198 aa overlap 4 1 10 22 [Reporter]SCO4531 (1H12)_PCR/1731 [Gene]SCO4531/1588 NC_003888 2SCD4.02, possible septum determining protein, len: 145 aa; similar to TR:CAB92601 (EMBL:AL356813) Streptomyces coelicolor putative septum determining protein MinD3, 131 aa; fasta scores: opt: 115 z-score: 155.7 E(): 0.33; 30.7% identity in 137 aa overlap. Contains possible hydrophobic membrane spanning region 1 3 5 16 [Reporter]SCO7320 (6I22)_PCR/4135 [Gene]SCO7320/3761 NC_003888 SC5F8.30c, unknown, len: 580 aa. Similar in parts to many Streptomyces coelicolor proteins of undefined function including: TR:Q9RJE1(EMBL:AL133171) putative regulatory protein, SCF81.10C (828 aa), fasta scores opt: 676 z-score: 710.0 E(): 4.5e-32 34.7% identity in 426 aa overlap and TR:Q9X8W1(EMBL:AL078610;) hypothetical 66.2 kd protein, SCH35.45C (634 aa), fasta scores opt: 633 z-score: 666.6 E(): 1.2e-29 33.4% identity in 539 aa overlap. 4 1 10 21 [Reporter]SCO2860 (2D12)_PCR/1730 [Gene]SCO2860/1587 NC_003888 SCE20.34c, arr, rifampin ADP-ribosyl transferase, len 136 aa. Highly similar to Mycobacterium smegmatis TR:O67972 (EMBL:AF001493) rifampin ADP-ribosyl transferase (143 aa), fasta scores opt: 487 z-score: 611.8 E(): 1.1e-26 53.0% identity in 134 aa overlap and Pseudomonas aeruginosa TR:O87490 (EMBL:AF078527) ribosylating transferase ARR-2 (150 aa), fasta scores opt: 429 z-score: 540.3 E(): 1e-22 50.0% identity in 128 aa overlap 1 3 5 15 [Reporter]SCO7430 (7E22)_PCR/4134 [Gene]SCO7430/3760 NC_003888 SC6D11.26, unknown, len: 228 aa. Contains a Pfam match to entry PF01955 DUF105, Domain of unknown function. 4 1 10 20 [Reporter]SCO1520 (3P8)_PCR/1729 [Gene]SCO1520/1586 NC_003888 SCL2.10c, ruvC, crossover junction endodeoxyribonuclease, len:188 aa; highly similar to SW:RUVC_MYCTU (EMBL:Z77724) Mycobacterium tuberculosis crossover junction endodeoxyribonuclease (EC 3.1.22.4) RuvC, 188 aa; fasta scores: opt: 704 z-score: 820.0 E(): 0; 60.7% identity in 178 aa overlap and to SW:RUVC_ECOLI (EMBL:X59551) Escherichia coli crossover junction endodeoxyribonuclease (EC 3.1.22.4) RuvC, 172 aa; fasta scores: opt: 381 z-score: 450.1 E(): 1.1e-17; 40.1% identity in 177 aa overlap. Contains match to Prosite entry PS01321 Crossover junction endodeoxyribonuclease ruvC signature 4 1 10 19 [Reporter]SCO6930 (4L8)_PCR/1728 [Gene]SCO6930/1585 NC_003888 SC1G8.02c, unknown, len: 1032 aa. Similar to a number of proteins involved in the posttranslational modification of the lantibiotics e.g. Bacillus subtilis SW:SPAB_BACSU (EMBL:L24075) subtilin biosynthesis protein, SpaB (1030 aa), fasta scores opt: 516 z-score: 571.0 E(): 2.4e-24 22.1% identity in 988 aa overlap. Also similar to Streptomyces coelicolor TR:CAB55531 (EMBL:AL117322) hypothetical 114.5 kd protein, SCF1.11 (1053 aa), fasta scores opt: 884 z-score: 982.0 E(): 0 30.6% identity in 1081 aa overlap. Contains a TTA leucine codon, possible target for bldA regulation 4 1 10 18 [Reporter]SCO6389 (5H8)_PCR/1727 [Gene]SCO6389/1584 NC_003888 SC3C8.08c, unknown, len: 130 aa 4 1 10 17 [Reporter]SCO2059 (6D8)_PCR/1726 [Gene]SCO2059/1583 NC_003888 SC4G6.28c, possible membrane protein, len: 238aa; Contains possible membrane spanning hydrophobic regions. 4 1 10 16 [Reporter]SCO5428 (7P4)_PCR/1725 [Gene]SCO5428/1582 NC_003888 SC6A11.04c, possible integral membrane transport protein, len: 296aa; similar to many proposed transport proteins e.g. TR:Q9X9Y6(EMBL:AL096743) putative integral membrane transport protein from Streptomyces coelicolor (298 aa) fasta scores: opt: 515, z-score: 609.9, E(): 1.5e-26, 33.9% identity in 295 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, Prosite matches to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and possible membrane-spanning hydrophobic regions 4 1 10 15 [Reporter]SCO7235 (8L4)_PCR/1724 [Gene]SCO7235/1581 NC_003888 SC2H12.34, possible integral membrane protein, partial CDS, len: >86 aa. Highly similar to proteins of unknown function from: Mycobacterium tuberculosis SW:YL99_MYCTU(EMBL:Z70283) (139 aa), fasta scores opt: 200 z-score: 283.7 E(): 2.5e-08 45.6% identity in 90 aa overlap and Streptomyces coelicolor TR:Q9X812(EMBL:AL049497) putative integral membrane protein, SC6G10.27C (132 aa), fasta scores opt: 245 z-score: 344.5 E(): 1e-11 43.0% identity in 86 aa overlap. Contains possible membrane spanning hydrophobic domains. Overlaps and extends into CDS SC7A12.02 on the adjoining cosmid.,SC7A12.02, possible integral membrane protein, len: 136 aa. Highly similar to Streptomyces coelicolor TR:Q9X812(EMBL:AL049497) putative integral membrane protein, SC6G10.27C (132 aa), fasta scores opt: 333 z-score: 421.2 E(): 5.4e-16 39.4% identity in 132 aa overlap and Mycobacterium tuberculosis TR:Y00A_MYCTU(EMBL:Z70283) hypothetical 14.9 kd protein (139 aa), fasta scores opt: 284 z-score: 361.2 E(): 1.2e-12 43.4% identity in 129 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 1 10 14 [Reporter]SCO7719 (9H4)_PCR/1723 [Gene]SCO7719/1580 NC_003888 SC8D11.10c, probable tetR-family transcriptional regulator, len: 219 aa; similar to SW:TCMR_STRGA (EMBL:M80674) Streptomyces glaucescens tetracenomycin C transcriptional repressor TcmR, 226 aa; fasta scores: opt: 249 z-score: 288.5 E(): 1.6e-08; 34.4% identity in 195 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a possible helix-turn-helix motif at residues 37..58 (+5.10 SD) 1 4 22 14 [Reporter]SCO5415 (9E13)_PCR/6522 [Gene]SCO5415/5939 NC_003888 SC8F4.19, icmA, isobutyryl-CoA mutase A, len: 566 aa; previously sequenced and characterised as TR:Q9X949 (EMBL:AJ237976). Contains Pfam match to entry PF01642 MM_CoA_mutase, Methylmalonyl-CoA mutase. 1 4 22 13 [Reporter]SCO1922 (10A13)_PCR/6521 [Gene]SCO1922/5938 NC_003888 SCC22.04c, possible ABC transporter ATP-binding subunit, len: 254aa; similar to many proposed ABC transporter subunit eg. SW:ABCX_CYAPA probable ATP-dependent transporter from Cyanophora paradoxa (259 aa) fasta scores; opt: 856, z-score: 945.3, E(): 0, (55.3% identity in 246 aa overlap). Also highly similar to TR:O53154 (EMBL:AL021184) hypothetical protein from Mycobacterium tuberculosis (266 aa) fasta scores; opt: 1258, z-score: 1383.7, E(): 0, (76.9% identity in 251 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 4 22 12 [Reporter]SCO4323 (11M9)_PCR/6520 [Gene]SCO4323/5937 NC_003888 SCD12A.06, possible hydrolase, len: 220 aa; similar to TR:O86530 (EMBL:AL031124) Streptomyces coelicolor putative hydrolase SC1C2.30, 244 aa; fasta scores: opt: 128 z-score: 155.4 E(): 0.34; 32.9% identity in 167 aa overlap. Contains Pfam match to entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase 1 4 22 11 [Reporter]SCO4642 (12I9)_PCR/6519 [Gene]SCO4642/5936 NC_003888 SCD82.13, unknown, len: 126 aa 1 4 22 9 [Reporter]SCO0586 (14A9)_PCR/6517 [Gene]SCO0586/5935 NC_003888 SCF55.10c, cvnC11, hypothetical protein, len: 113 aa; similar to various hypothetical proteins, e.g: TR:CAB41560 (EMBL:AL049727) Streptomyces coelicolor hypothetical 12.8 KD protein SC9B1.14c, 119 aa; fasta scores:opt: 264 z-score: 363.7 E(): 7e-13; 51.8% identity in 112 aa overlap 1 4 22 8 [Reporter]SCO2946 (15M5)_PCR/6516 [Gene]SCO2946/5934 NC_003888 SCE59.05c, possible sugar transporter sugar-binding protein, len: 432 aa; similar to TR:CAB59588 (EMBL:AL132662) Streptomyces coelicolor putative sugar transporter sugar-binding protein SCF11.11, 422 aa; fasta scores: opt: 538 z-score: 581.0 E(): 6e-25; 27.0% identity in 429 aa overlap and to SW:MALX_STRPN (EMBL:L08611) Streptococcus pneumoniae maltose/maltodextrin-binding protein precursor MalX, 423 aa; fasta scores: opt: 299 z-score: 325.5 E(): 1e-10; 26.0% identity in 431 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide sequence 1 4 22 7 [Reporter]SCO0375 (16I5)_PCR/6515 [Gene]SCO0375/5933 NC_003888 SCF62.01c, possible integral membrane transport protein, partial CDS, len: > 406 aa. Similar to many members of the drug resistance translocase family e.g. Staphylococcus aureus SW:QACA_STAAU (EMBL; X56628) antiseptic resistance protein (514 aa), fasta scores opt: 750 z-score: 750.4 E():0 30.0% identity in 493 aa overlap, as well as many other Streptomyces coelicolor membrane transport proteins e.g. TR:O54186 (EMBL; AL021411) SC7H1.27 (533 aa), fasta scores opt: 972 z-score: 970.2 E(): 0 35.0% identity in 543 aa overlap. Overlaps with and extends into SCF41.34c. Contains multiple possible membrane spanning hydrophobic domains and a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter.,SCF41.34c, possible transport protein, partial CDS, len: >157 aa. Similar to Streptomyces coelicolor putative transport protein TR:O54186 (EMBL; AL021411) putative transport protein SC7H1.27 (533 aa), fasta scores opt: 263 z-score: 281.1 E(): 2.6e-08 36.1% identity in 166 aa overlap. 1 4 22 6 [Reporter]SCO3652 (17E5)_PCR/6514 [Gene]SCO3652/5932 NC_003888 SCH10.30c, putative membrane protein, len: 310 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains possible hydrophobic membrane spanning region at the C-terminal domain 1 3 5 14 [Reporter]SCO6893 (8A22)_PCR/4133 [Gene]SCO6893/3759 NC_003888 SC7F9.45, unknown, len: 103 aa. 1 4 22 5 [Reporter]SCO1834 (18A5)_PCR/6513 [Gene]SCO1834/5931 NC_003888 SCI8.19c, unknown, len: ,124 aa. 1 3 5 13 [Reporter]SCO6440 (9M18)_PCR/4132 [Gene]SCO6440/3758 NC_003888 SC9B5.07, conserved hypothetical protein, len: 250aa; similar to 3 eg. SW:YA99_METJA hypothetical protein MJ1099 from Methanococcus jannaschii (235 aa) fasta scores;opt: 848, z-score: 1229.1, E(): 0, (57.9% identity in 233 aa overlap). 1 4 22 4 [Reporter]SCP1.148 (19M1)_PCR/6512 [Gene]SCP1.148/5930 NC_003888 SCP1.148, possible secreted protein, len: 384aa; similar to many especially TR:Q9L1N1 (EMBL:AL138977) putative secreted protein from Streptomyces coelicolor (377 aa) fasta scores; opt: 1234, z-score: 1291.1, E(): 0, 52.2% identity in 385 aa overlap. Contains a possible cleavable N-terminal signal sequence. Also contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family. 1 3 5 12 [Reporter]SCO1957 (10I18)_PCR/4131 [Gene]SCO1957/3757 NC_003888 SCC54.17, probable fructokinase, len: 302 aa; similar to many e.g. SW:SCRK_RHILT Frk, fructokinase from Rhizobium leguminosarum biovar trifolii (326 aa) fasta scores; opt: 617, z-score: 682.4, E(): 1.1e-30, (41.0% identity in 288 aa overlap). Contains PS00584 pfkB family of carbohydrate kinases signature 2 and Pfam match to entry PF00294 pfkB, pfkB family carbohydrate kinase, score 132.90, E-value 9.6e-41 1 3 5 11 [Reporter]SCO4366 (11E18)_PCR/4130 [Gene]SCO4366/3756 NC_003888 SCD19.21, possible phosphoserine aminotransferase, len: 363 aa; similar to SW:SERC_MYCTU (EMBL:Z73101) Mycobacterium tuberculosis putative phosphoserine aminotransferase (EC 2.6.1.52) Serc or MTCY31.12c, 376 aa; fasta scores: opt: 1693 z-score: 1994.6 E(): 0; 66.9% identity in 363 aa overlap 1 3 5 9 [Reporter]SCO3166 (13M14)_PCR/4128 [Gene]SCO3166/3755 NC_003888 SCE87.17c, possible membrane transport protein, len: 745 aa. Highly similar to several other Streptomyces coelicolor putative transport proteins: TR:CAB56653 (EMBL:AL121596) putative membrane transport protein SCF51A.01C (765 aa), fasta scores opt: 1664 z-score: 1670.0 E(): 0 57.8% identity in 760 aa overlap and TR:Q9Z577(EMBL:AL035569) putative integral membrane export protein SC8D9.13 (748 aa), fasta scores opt: 1382 z-score: 1387.5 E():0 40.7% identity in 734 aa overlap. Also similar to a number of other putative MMPL family membrane proteins such as: Mycobacterium leprae SW:MMLB_MYCLE(EMBL:Z95398) putative membrane protein MMPL11 (1014 aa), fasta scores opt: 999 z-score: 1001.8 E(): 0 29.6% identity in 743 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence. 1 3 5 8 [Reporter]SCO3901 (14I14)_PCR/4127 [Gene]SCO3901/3754 NC_003888 SCH24.23, probable secreted penicillin-binding protein, len: 795 aa; similar to many e.g. SW:PBPF_BACSU (EMBL:Y14083), PbpF, Bacillus subtilis penicillin-binding protein 1A (714 aa), fasta scores; opt: 439 z-score: 369.7 E(): 2.8e-13, 31.2% identity in 683 aa overlap. Contains hydrophobic, possible membrane-spanning region at N-terminus. Contains Pfam matches to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain, score 49.60, E-value 2.8e-11 and entry PF00912 Transglycosyl, Transglycosylase, score 172.40, E-value 7.3e-48. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 5 7 [Reporter]SCO3864 (15E14)_PCR/4126 [Gene]SCO3864/3753 NC_003888 SCH18.01, probable transcriptional regulator (fragment), len: >70 aa; similar to TR:Q9X8D7 (EMBL:AL049573) Streptomyces coelicolor putative GntR-family transcriptional regulator SCE39.14c, 243 aa; fasta scores: opt: 142 z-score: 189.8 E(): 0.0042; 36.8% identity in 57 aa overlap. Overlaps with Streptomyces coelicolor putative transcriptional regulator (fragment) SCH69.34, 225 aa,SCH69.34, probable transcriptional regulator, len: >225aa; similar to many egs. TR:Q07191 (EMBL:Z19589) KorSA regulatory protein from the pSAM2 plasmid of Streptomyces ambofaciens (259 aa) fasta scores; opt: 224, z-score: 275.8, E(): 4.9e-08, (34.3% identity in 233 aa overlap) and SW:HUTC_KLEAE repressor for the histidine utilization genes of Klebsiella aerogenes (241 aa) fasta scores; opt: 180, z-score: 223.7, E(): 3.9e-05, (30.4% identity in 214 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and probable helix-turn-helix 29-50aa (+3.13 SD). 4 1 10 13 [Reporter]SCO5125 (10D4)_PCR/1722 [Gene]SCO5125/1579 NC_003888 SC9E12.10c, hypothetical protein, len: 287 aa; similar to TR:Q9RDR4 (EMBL:AL136500) Streptomyces coelicolor hypothetical 31.7 kDa protein SC1G2.15c, 284 aa; fasta scores: opt: 1240 z-score: 1466.1 E(): 0; 67.2% identity in 287 aa overlap 1 3 5 6 [Reporter]SCO0639 (16A14)_PCR/4125 [Gene]SCO0639/3752 NC_003888 SCF56.23c, probable integral membrane protein, len: 310 aa; similar to TR:O86628 (EMBL:AL031155) Streptomyces coelicolor putative integral membrane protein SC3A7.27, 309 aa; fasta scores: opt: 577 z-score: 631.4 E(): 8.7e-28; 41.9% identity in 298 aa overlap 4 1 10 12 [Reporter]SCO2697 (10P24)_PCR/1721 [Gene]SCO2697/1578 NC_003888 SCC61A.18c, possible secreted protein, len: 192aa; contains a possible cleavable N-terminal signal sequence. 1 3 5 5 [Reporter]SCO3840 (17M10)_PCR/4124 [Gene]SCO3840/3751 NC_003888 SCH69.10c, unknown, len: 271aa; similar to TR:P74322 (EMBL:D90914) from Synechocystis sp. PCC6803 (228 aa) fasta scores; opt: 238, z-score: 277.0, E(): 4.2e-08, (26.6% identity in 259 aa overlap). Contains possible coiled-coil at 30-40aa. 4 1 10 11 [Reporter]SCO4440 (11L24)_PCR/1720 [Gene]SCO4440/1577 NC_003888 SCD6.18, hypothetical protein, len: 252 aa; similar to TR:Q9X7M7 (EMBL:AL049587) Streptomyces coelicolor putative integral membrane protein SC5F2A.04, 302 aa; fasta scores: opt: 228 z-score: 237.1 E(): 9.5e-06; 32.6% identity in 224 aa overlap 1 3 5 4 [Reporter]SCO0283 (18I10)_PCR/4123 [Gene]SCO0283/3750 NC_003888 SCF85.11c, unknown, len: 164 aa. 4 1 10 10 [Reporter]SCO3510 (12H24)_PCR/1719 [Gene]SCO3510/1576 NC_003888 SCE134.11c, possible DNA methylase, len: 248 aa; similar to many e.g. TR:O68789 (EMBL:AF053947) adenine methyltransferase homolog from Yersinia pestis (213 aa) fasta scores; opt: 246, z-score: 300.2, E(): 2.1e-09, (30.8% identity in 224 aa overlap). Contains Prosite match to PS00092 N-6 Adenine-specific DNA methylases signature. 4 1 10 9 [Reporter]SCO1766 (13D24)_PCR/1718 [Gene]SCO1766/1575 NC_003888 SCI51.06c, possible glycohydrolase, len: 339 aa; shows weak similarity to ADP-ribosyl glycohydrolases from nitrogen-fixing bacteria e.g. SW:DRAG_RHORU (EMBL:X16187), DraG, Rhodospirillum rubrum ADP-ribosylglycohydrolase (294 aa), fasta scores; opt: 211 z-score: 253.7 E(): 8.9e-07, 25.6% identity in 332 aa overlap 4 1 10 8 [Reporter]SCO1795 (14P20)_PCR/1717 [Gene]SCO1795/1574 NC_003888 SCI5.03, unknown, len: 168 aa; similar to TR:P72833 (EMBL:D90901) hypothetical protein from Synechocystis sp. PCC6803 (165 aa) fasta scores; opt: 417, z-score: 517.5, E(): 1.7e-21, (46.9% identity in 143 aa overlap) 4 1 10 7 [Reporter]SCO0680 (15L20)_PCR/1716 [Gene]SCO0680/1573 NC_003888 SCF15.01c, possible transmembrane efflux protein, partial CDS, len: > 443 aa. Highly similar to many members of the drug resistance translocase family including: Streptomyces lipmanii (Streptomyces alboniger) SW:PUR8_STRLP(EMBL:X76855) puromycin resistance protein Pur8 (503 aa), fasta scores opt: 902 z-score: 986.1 E():0 42.6% identity in 420 aa overlap and Streptomyces coelicolor SW:AC22_STRCO(EMBL:M64683) probable actinorhodin transporter ActII-2 (578 aa), fasta scores opt: 867 z-score: 947.3 E():0 39.6% identity in 417 aa overlap. Contains a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and multiple possible membrane spanning hydrophobic domains.,SCF91.40c, possible transmembrane efflux protein, partial CDS, len: > 105 aa. Similar to Streptomyces coelicolor TR:CAB42035(EMBL:AL049754) putative transmembrane efflux protein SCH10.26C (484 aa), fasta scores pt: 111 z-score: 141.1 E():1.7 31.4% identity in 105 aa overlap. Contains a possible membrane spanning hydrophobic domain. Overlaps and extends into CDS SCF15.01c on the adjoining cosmid. 4 1 10 6 [Reporter]SCO1044 (16H20)_PCR/1715 [Gene]SCO1044/1572 NC_003888 SCG20A.24c, possible secreted protein, len: 313 aa. Contains possible N-terminal region signal peptide sequence 4 1 10 5 [Reporter]SCO0391 (17D20)_PCR/1714 [Gene]SCO0391/1571 NC_003888 SCF62.17, possible transferase, len: 413 aa. Similar to many including Streptomyces coelicolor TR:Q9Z5B7 (EMBL:AL035478) putative transferase SC2G5.06 (406 aa), fasta scores opt: 263 z-score: 301.1 E(): 2.1e-09 31.9% identity in 385 aa overlap and Erwinia amylovora SW:AMSK_ERWAM (EMBL:X77921) amylovoran biosynthesis protein AmsK (407 aa), fasta scores opt: 224 z-score: 257.0 E(): 5.8e-07 28.4% identity in 232 aa overlap. Contains a Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1 4 1 10 4 [Reporter]SCP1.183c (18P16)_PCR/1713 [Gene]SCP1.183c/1570 NC_003888 SCP1.183c, unknown, len: 234aa; weakly similar to TR:O07246 (EMBL:Z96800) hypothetical protein from Mycobacterium tuberculosis (220 aa) fasta scores; opt: 129, z-score: 156.4, E(): 0.3, 29.2% identity in 161 aa overlap. 1 4 22 3 [Reporter]SCO0006 (20I1)_PCR/6511 [Gene]SCO0006/5929 NC_003888 SC1C9.03, possible ATP/GTP-binding protein (fragment), len: >223 aa; identical to N-terminal part of TR:Q9FBV7 (EMBL:AL391338) Streptomyces coelicolor putative ATP/GTP-binding protein, 368 aa; situated on the opposite end of the chromosome. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop),SCJ30.01, partial CDS, unknown, len: >183 aa; weakly similar to the C-terminal half of suspected transposition related nucleotide binding proteins eg. TR:Q57461 (EMBL:U49101) TniB delta1 from plasmid pVS1 (286 aa) fasta scores; opt: 133, z-score: 170.1, E(): 0.041, (29.7% identity in 148 aa overlap). 1 4 21 22 [Reporter]SCO1135 (1A21)_PCR/6508 [Gene]SCO1135/5928 NC_003888 2SCG38.28, probable tetR-family transcriptional regulator, len: 196 aa; highly similar to TR:Q9RJL5 (EMBL:AL121849) Streptomyces coelicolor putative tetR-family transcriptional regulator SCF6.16, 194 aa; fasta scores: opt: 461 z-score: 557.4 E(): 1.3e-23; 40.7% identity in 189 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 38..59 (+4.25 SD) 1 4 21 21 [Reporter]SCO2266 (2M17)_PCR/6507 [Gene]SCO2266/5927 NC_003888 SCC75A.12, map3, methionine aminopeptidase (EC 3.4.11.18), len: 285 aa. Highly similar to many methionine aminopeptidases including: Escherichia coli SW:AMPM_ECOLI (EMBL:M15106) (264 aa), fasta scores opt: 806 z-score: 892.1 E(): 0 49.6% identity in 246 aa overlap and Mycobacterium tuberculosis TR:O33343 (EMBL:AL008883) (285 aa), fasta scores opt: 1385 z-score: 1525.3 E():0 70.7% identity in 283 aa overlap. Also highly similar to many Eukaryotic methionine aminopeptidases e.g. Homo sapiens (Human) SW:AMP1_HUMAN (EMBL:D42084) (349 aa), fasta scores opt: 857 z-score: 945.3 E():0 47.5% identity in 280 aa overlap. Map3 is also similar to two other putative Map proteins from Streptomyces coelicolor: TR:O54208 (EMBL:X83011) Map, SC6G4.02 (278 aa), fasta scores opt: 455 z-score: 459.7 E(): 3.1e-20 38.6% identity in 259 aa overlap and TR:O69937 (EMBL:AL023861) Map2, SC3C8.28c (258 aa), fasta scores opt:565 z-score: 569.1 E(): 2.5e-26 37.8% identity in 251 aa overlap. Contains a Pfam match to entry PF00557 Peptidase_M24, metallopeptidase family M24. 1 4 21 20 [Reporter]SCO7698 (3I17)_PCR/6506 [Gene]SCO7698/5926 NC_003888 SC1A4.06c, probable merR-family transcriptional regulator, len: 380 aa; N-terminal region similar to SW:TIPA_STRLI (EMBL:S64314) Streptomyces lividans transcriptional activator TipA, 253 aa; fasta scores: opt: 404 z-score: 467.3 E(): 1.7e-18; 40.0% identity in 185 aa overlap and C-terminal region similar to TR:O87011 (EMBL:U93274) Pseudomonas aeruginosa hypothetical protein YafE, 187 aa; blastp scores: Score= 121 (42.6 bits), Expect= 1.6e-05, P= 1.6e-05; Identities= 51/144 (35%), Positives= 72/144 (50%). Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and possible helix-turn-helix motif at residues 14..35 (+3.15 SD) 1 4 21 19 [Reporter]SCO5593 (4E17)_PCR/6505 [Gene]SCO5593/5925 NC_003888 SC2E1.10, probable 16S rRNA processing protein, len: 188 aa; similar to hypothetical proteins from many organisms e.g. YX38_MYCTU MTCY274.38c (176 aa), fasta scores; opt: 479 z-score: 693.1 E(): 2.3e-31, 48.3% identity in 176 aa overlap. Also some similarity to RIMM_HAEIN 16S rRNA processing protein rimM (178 aa), fasta scores; opt: 210 z-score: 174.2 E(): 0.018, 27.6% identity in 170 aa overlap and to SW:RIMM_ECOLI (EMBL:X01818) Escherichia coli 16S rRNA processing protein RimM or B2608, 182 aa; fasta scores: opt: 216 Z-score: 258.0 E(): 9.8e-07; 24.561% identity in 171 aa overlap 1 4 21 18 [Reporter]SCO2403 (5A17)_PCR/6504 [Gene]SCO2403/5924 NC_003888 SC4A7.31, hypothetical protein, len: 323 aa; similar to TR:O27045 (EMBL:AE000870) Methanobacterium thermoautotrophicum hypothetical 32.8 kD protein MTH964, 299 aa; fasta scores: opt: 685 z-score: 763.8 E(): 0; 40.3% identity in 300 aa overlap 1 4 21 17 [Reporter]SCO6742 (6M13)_PCR/6503 [Gene]SCO6742/5923 NC_003888 SC5F2A.25, probable ABC transporter, len: 547aa; similar to many eg. TR:Q53912 (EMBL:X84374) Ard1, ABC transporter which confers resistance to the aminonucleoside antibiotic A201A in Streptomyces capreolus (558 aa) fasta scores; opt: 1561, z-score: 1661.3, E(): 0, (46.7% identity in 542 aa overlap). Contains two Pfam matches to entry PF00005 ABC_tran, ABC transporter, two Prosite matches to PS00017 ATP/GTP-binding site motif A (P-loop) and Prosite match to PS00211 ABC transporters family signature. 1 4 21 16 [Reporter]SCO1426 (7I13)_PCR/6502 [Gene]SCO1426/5922 NC_003888 SC6D7.13c, unknown, len: 145 aa. 1 3 5 3 [Reporter]SCO7511 (19E10)_PCR/4122 [Gene]SCO7511/3749 NC_003888 SCBAC25F8.03, gap2, glyceraldehyde 3-phosphate dehydrogenase, len: 332 aa; highly similar to SW:G3P_STRAU (EMBL:U21191) Streptomyces aureofaciens glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) Gap, 332 aa; fasta scores: opt: 1898 Z-score: 2070.6 bits: 391.5 E(): 1e-107; 88.855% identity in 332 aa overlap and to TR:CAB38137 (EMBL:AL035591) Streptomyces coelicolor glyceraldehyde 3-phosphate dehydrogenase Gap or SCC54.07c, 337 aa; fasta scores: opt: 1272 Z-score: 1174.4 bits: 225.7 E(): 1.1e-59; 57.831% identity in 332 aa overlap. Contains Pfam matches to entries PF00044 gpdh, Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain and PF02800 gpdh_C, Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain and match to Prosite entry PS00071 Glyceraldehyde 3-phosphate dehydrogenase active site 1 4 21 15 [Reporter]SCO5209 (8E13)_PCR/6501 [Gene]SCO5209/5921 NC_003888 SC7E4.06, possible tetR-family transcriptional regulator, len: 203 aa; similar to TR:Q9Z551 (EMBL:AL035212) Streptomyces coelicolor putative transcriptional regulator SC9B2.08c, 192 aa; fasta scores: opt: 169 z-score: 206.9 E(): 0.00047; 31.9% identity in 182 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 24..45 (+3.85 SD) 1 3 5 2 [Reporter]SCO1877 (20M6)_PCR/4121 [Gene]SCO1877/3748 NC_003888 SCI39.24, unknown, len: 224 aa 1 4 21 14 [Reporter]SCO5410 (9A13)_PCR/6500 [Gene]SCO5410/5920 NC_003888 SC8F4.14c, hypothetical protein, improbable, len: 41 aa 1 3 4 22 [Reporter]SCO4545 (1M2)_PCR/4119 [Gene]SCO4545/3747 NC_003888 2SCD4.16, hypothetical protein, len: 304 aa; identical to previously sequenced TR:Q9XD94 (EMBL:AF106004) Streptomyces coelicolor hypothetical 34.1 kDa protein, 304 aa and highly similar to TR:CAB81866 (EMBL:AL161691) Streptomyces coelicolor hypothetical protein SCD40A.21c, 302 aa; fasta scores: opt: 850 z-score: 894.4 E(): 0; 45.0% identity in 300 aa overlap 1 3 4 21 [Reporter]SCO5366 (2I2)_PCR/4118 [Gene]SCO5366/3746 NC_003888 2SC6G5.10, atpI, ATP synthase protein I, len: 144 aa; identical to SW:P50015 (ATPZ_STRLI) ATP synthase protein I from Streptomyces lividans (144 aa). Contains possible membrane-spanning hydrophobic regions 1 3 4 20 [Reporter]SCO1620 (3E2)_PCR/4117 [Gene]SCO1620/3745 NC_003888 SCI41.03c, opuABC glycine betaine transport system permease protein, len: 871 aa. N-terminal region highly similar to SW:OPUB_BACSU (P46921) Bacillus subtilis glycine betaine transport system permease protein ; fasta scores: opt: 847 z-score: 884.1 E(): 0; 47.2% identity in 269 aa overlap, and C-terminal region similar to SW:OPUC_BACSU glyine betaine-binding protein precusor; fasta scores; opt: 322 z-score: 339.7 E(): 1.5e-11; 32.8% identity in 271 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, binding-protein-dependent transport systems inner membrane component and prosite match to entry PS00402 Binding-protein-dependent transport systems inner membrane component sign 1 3 4 19 [Reporter]SCO6162 (4A2)_PCR/4116 [Gene]SCO6162/3744 NC_003888 SC1A9.26c, probable luxR family two-component system response regulator, len: 260aa; similar to a family of response regulators eg. TR:O69961 (EMBL:AL022268) probable two-component transcriptional regulator from Streptomyces coelicolor (219 aa) fasta scores; opt:1079, z-score: 1434.4, E():0, (77.5% identity in 218 aa overlap) and DEGU_BACBR (EMBL:L15444) transcriptional activator protein DegU from Bacillus brevis (236 aa) fasta scores; opt:378, z-score: 682.5, E(): 9.7e-31, (36.6% identity in 227 aa overlap). Contains PS00622 Bacterial regulatory proteins, luxR family signature, Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 99.70, E-value 5.6e-26, Pfam match to entry PF00072 response_reg, Response regulator receiver domain, score 132.90, E-value 5.6e-36 and an helix-turn-helix motif from: 1 to: 260, Score 1221 (+3.35 SD). 1 3 4 18 [Reporter]SCO2025 (4M22)_PCR/4115 [Gene]SCO2025/3743 NC_003888 SC3A3.03c, gltD, probable glutamate synthase small subunit, len: 487 aa; similar to bacterial glutamate synthases e.g. TR:Q51584 (EMBL:D85230), gltD, Plectonema boryanum small subunit of NADH-dependent glutamate synthase (492 aa), fasta scores; opt: 1295 z-score: 1405.0 E(): 0, 51.2% identity in 500 aa overlap. Also similar to part of eukaryotic glutamate synthases e.g. SW:GLSN_MEDSA (EMBL:L01660) Medicago sativa (Alfalfa) Glutamate synthase [NADH] precursor (2194 aa) (49.1% identity in 489 aa overlap). Contains Pfam match to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains 4 1 10 3 [Reporter]SCO7517 (19L16)_PCR/1712 [Gene]SCO7517/1569 NC_003888 SCBAC25F8.09, possible integral membrane protein, len: 432 aa; similar to TR:Q9HTD6 (EMBL:AE004955) Pseudomonas aeruginosa hypothetical protein PA5430, 404 aa; fasta scores: opt: 1026 Z-score: 917.9 bits: 178.8 E(): 1.6e-43; 43.557% identity in 388 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 4 17 [Reporter]SCO7056 (5I22)_PCR/4114 [Gene]SCO7056/3742 NC_003888 SC4G1.22c, possible gntR-family transcriptional regulator, len: 253 aa; similar to TR:Q9RYU6 (EMBL:AE001863) Deinococcus radiodurans transcriptional regulator, gntR-family DRA0211, 279 aa; fasta scores: opt: 217 z-score: 236.0 E(): 1.1e-05; 37.6% identity in 141 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and match to Prosite entry PS00043 Bacterial regulatory proteins, gntR family signature. Also contains a possible helix-turn-helix at residues 47..68 (+4.70 SD) 4 1 10 2 [Reporter]SCO3972 (20D16)_PCR/1711 [Gene]SCO3972/1568 NC_003888 SCBAC25E3.09, conserved hypothetical protein, len: 221aa: similar to TR:Q9K3U7 (EMBL:AL359988) conserved hypothetical protein SCD10.30c from Streptomyces coelicolor fasta scores; opt: 367, Z-score: 407.8, 35.354% identity (37.037% ungapped) in 198 aa overlap. 1 3 4 16 [Reporter]SCO7588 (6E22)_PCR/4113 [Gene]SCO7588/3741 NC_003888 SC5F1.42c, possible oxidoreductase, len: 306 aa; similar to various Streptomyces coelicolor putative oxidoreductases/dehydrogenases, e.g. TR:Q9RIU1 (EMBL:AL133278) putative dehydratase SCM11.21c, 319 aa; fasta scores: opt: 912 z-score: 984.0 E(): 0; 47.4% identity in 312 aa overlap 4 1 9 22 [Reporter]SCO2806 (1D12)_PCR/1709 [Gene]SCO2806/1567 NC_003888 2SCC13.14c, possible mutase, len: 198 aa; similar to TR:O05852 (EMBL:Z95120) Mycobacterium tuberculosis hypothetical 21.9 kDa protein MTCY07D11.12c, 203 aa; fasta scores: opt: 487 z-score: 602.4 E(): 4.7e-26; 42.3% identity in 194 aa overlap. Contains Pfam match to entry PF00300 PGAM, Phosphoglycerate mutase family 1 3 4 15 [Reporter]SCO7425 (7A22)_PCR/4112 [Gene]SCO7425/3740 NC_003888 SC6D11.21, possible multidrug-efflux transporter protein, len: 577 aa. Highly similar to several including: Streptomyces virginiae TR:Q9XE01(EMBL:AB019519) virginiamycin S resistance protein, VarS (518 aa), fasta scores opt: 322 z-score: 328.6 E(): 7e-11 25.7% identity in 536 aa overlap and Deinococcus radiodurans TR:AAF11851(EMBL:AE002062) multidrug-efflux transporter, putative (410 aa), fasta scores opt: 937 z-score: 945.6 E(): 0 41.3% identity in 395 aa overlap. Contains a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and multiple possible membrane spanning hydrophobic domains. 4 1 9 21 [Reporter]SCO5863 (2P8)_PCR/1708 [Gene]SCO5863/1566 NC_003888 SC2E9.04, cutS, two-component sensor (kinase), len: 414 aa; identical to SW:CUTS_STRLI (EMBL:Q03757) CutS protein from Streptomyces lividans (414 aa). Contains Pfam match to entry signal PF00512, Signal C terminal domain, score 191.45. Contains possible hydrophobic membrane spanning regions 4 1 9 20 [Reporter]SCO2563 (3L8)_PCR/1707 [Gene]SCO2563/1565 NC_003888 SCC123.01, rspT, 30s ribosomal protein S20, len: 88 aa. Highly similar to Bacillus subtilis SW:RS20_BACSU(EMBL:D84432) 30S ribosomal protein S20 (BS20) (87 aa), fasta scores opt: 226 z-score: 298.8 E(): 3e-09 44.0% identity in 84 aa overlap and Mycobacterium tuberculosis SW:RS20_MYCTU(EMBL:Z81368) 30S ribosomal protein S20 (86 aa), fasta scores opt: 363 z-score: 465.7 E(): 1.5e-18 70.9% identity in 86 aa overlap. Contains a Pfam match to entry PF01649 Ribosomal_S20p, Ribosomal protein S20. 4 1 9 19 [Reporter]SCO5533 (4H8)_PCR/1706 [Gene]SCO5533/1564 NC_003888 SC1C2.14c, unknown, len: 185 aa; similar to hypothetical proteins from E. coli YCEI_ECOLI (191 aa), fasta scores; opt: 373 z-score: 567.7 E(): 2.3e-24, 34.1% identity in 179 aa overlap and H. pylori TR:O25873 (EMBL:AE000633) HP1286 (182 aa), fasta scores; opt: 279 z-score: 242.2 E(): 3.1e-06, 27.9% identity in 172 aa overlap 4 1 9 18 [Reporter]SCO6385 (5D8)_PCR/1705 [Gene]SCO6385/1563 NC_003888 SC3C8.04c, probable integral membrane protein, len: 644 aa; similar to hypothetical proteins e.g. M. tuberculosis TR:O07192 (EMBL:Z96072) MTCY05A6.11C (647 aa), fassta scores; opt: 916 z-score: 1168.8 E(): 0, 35.3% identity in 666 aa overlap. Also similar to S.coelicolor SC10A5.05 (E():0, 34.8% identity in 669 aa overlap) 4 1 9 17 [Reporter]SCO6678 (6P4)_PCR/1704 [Gene]SCO6678/1562 NC_003888 SC5A7.28c, probable iron-sulfur oxidoreductase beta subunit, len: 313 aa; similar to many e.g. POBB_PSEPS phenoxybenzoate dioxygenase beta subunit (Pseudomonas pseudoalcaligenes) (319 aa), fasta scores; opt: 824 z-score: 993.1 E(): 0, 45.9% identity in 303 aa overlap. C-terminus is a 2Fe-2S ferredoxin domain similar to e.g. FERV_ANAVA ferredoxin, vegetative (Anabaena variabilis) (98 aa), fasta scores; opt: 187 z-score: 222.4 E(): 3.8e-05, 38.2% identity in 68 aa overlap. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature and Pfam match to entry PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains, score 35.50, E-value 1.2e-06 4 1 9 16 [Reporter]SCO3621 (7L4)_PCR/1703 [Gene]SCO3621/1561 NC_003888 SC66T3.32c, probable serine-threonine protein kinase, len: 783 aa; N-terminal half similar to the corresponding region of several serine-threonine protein kinases e.g. SW:AFSK_STRCO (EMBL:D15062), afsK, S.coelicolor serine-threonine protein kinase (799 aa), fasta scores; opt: 944 z-score: 519.3 E(): 1.3e-21, 45.6% identity in 379 aa overlap. The central region contains 26x slightly degenerate GG(A/V)G(P/S) repeats. Contains two Pfam matches to entry PF00069 pkinase, Eukaryotic protein kinase domain, PS00108 Serine/Threonine protein kinases active-site signature and PS00107 Protein kinases ATP-binding region signature and possible hydrophobic membrane spanning region at the C-terminal domain 4 1 9 15 [Reporter]SCO4733 (8H4)_PCR/1702 [Gene]SCO4733/1560 NC_003888 SC6G4.11, probable integral membrane protein, len: 4 11 aa; some similarity to hypothetical proteins from M. tuberculosis and M. leprae e.g. TR:P71970 (EMBL:) MTCY441.42 ( Rv2673) (433 aa), fasta scores; opt: 383 z-score: 365.0 E() : 4.6e-13, 29.6% identity in 422 aa overlap 1 4 21 13 [Reporter]SCO2526 (10M9)_PCR/6499 [Gene]SCO2526/5919 NC_003888 SCC121.29c, possible acetyltransferase, len: 184 aa; similar to TR:O27080 (EMBLAE000872:) Methanobacterium thermoautotrophicum N-terminal acetyltransferase complex, subunit Ard1 MTH999, 156 aa; fasta scores: opt: 232 z-score: 289.3 E(): 1e-08; 29.6% identity in 152 aa overlap and to TR:CAB50578 (EMBL:AJ248288) Pyrococcus abyssi N-terminal acetyltransferase, 172 aa; fasta scores: opt: 213 z-score: 266.0 E(): 2e-07; 30.3% identity in 142 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf 1 4 21 12 [Reporter]SCO4402 (11I9)_PCR/6498 [Gene]SCO4402/5918 NC_003888 SCD10.34, unknown, len: 166 aa 1 4 21 11 [Reporter]SCO4477 (12E9)_PCR/6497 [Gene]SCO4477/5917 NC_003888 SCD65.20c, probable merR-family transcriptional regulatory protein, len: 238 aa; presents two hits to TR:Q9RKM5 (EMBL:AL138978) Streptomyces coelicolor putative merR family transcriptional regulator SCD17.06c, 319 aa; blastp scores for N-terminal domain: Score= 143 (50.3 bits), Expect= 7.3e-08, P= 7.3e-08; Identities= 42/108 (38%), Positives= 57/108 (52%) and blastp scores for C-terminal domain: Score= 102 (35.9 bits), Expect= 0.0052, P= 0.0052; Identities= 33/96 (34%), Positives= 45/96 (46%). Contains 2x Pfam matches to entry PF00376 merR, Bacterial regulatory proteins, merR family and also two possible helix-turn-helix motives at residues 8..29 (+4.71 SD) and 130..151 (+4.79 SD) 1 4 21 9 [Reporter]SCO3696 (14M5)_PCR/6495 [Gene]SCO3696/5916 NC_003888 SCH35.28c, probable transcriptional regulator, len: 122aa; similar to many egs. TR:P71941 (EMBL:Z80225) putative transcriptional regulator from Mycobacterium tuberculosis (126 aa) fasta scores; opt: 390, z-score: 503.7, E(): 1e-20, (56.6% identity in 122 aa overlap) and SW:ARSR_ECOLI arsenical resistance operon repressor from Escherichia coli (117 aa) fasta scores; opt: 198, z-score: 263.7, E(): 2.3e-07, (38.8% identity in 80 aa overlap). Contains Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family. 1 4 21 8 [Reporter]SCO2881 (15I5)_PCR/6494 [Gene]SCO2881/5915 NC_003888 SCE6.18, cvnC12, unknown, len: 117 aa. Similar to many proteins of undefined function from Streptomyces coelicolor e.g. TR:CAB71243 (EMBL:AL138598) SC1A2.05 (126 aa), fasta scores opt: 194 z-score: 251.3 E(): 1.4e-06 38.7% identity in 119 aa overlap and TR:Q9X834 (EMBL:AL049727) SC9B1.14C (119 aa), fasta scores opt: 192 z-score: 249.3 E(): 1.9e-06 37.9% identity in 124 aa overlap. 1 4 21 7 [Reporter]SCO0838 (16E5)_PCR/6493 [Gene]SCO0838/5914 NC_003888 SCF43A.28, conserved hypothetical protein, len: 141 aa; unknown function, similar to SW:YY13_MYCTU (EMBL:Z73966) Mycobacterium tuberculosis hypothetical protein (137 aa), fasta scores; opt: 393 z-score: 495.0 E(): 3.1e-20, 52.9% identity in 121 aa overlap Weakly similar to SC6G9.21 (EMBL:AL079356) S.coelicolor conserved hypothetical protein (144 aa) (35.7% identity in 126 aa overlap) 1 4 21 6 [Reporter]SCO0209 (17A5)_PCR/6492 [Gene]SCO0209/5913 NC_003888 SCJ12.21, unknown, len: 134 aa. 1 4 21 5 [Reporter]SCO1186 (18M1)_PCR/6491 [Gene]SCO1186/5912 NC_003888 SCG11A.17, probable lacI-family transcriptional regulator, len: 341 aa; similar to TR:CAB46346 (EMBL:AJ009798) Streptomyces reticuli regulatory protein CebR, 350 aa; fasta scores: opt: 1241 z-score: 1440.4 E(): 0; 57.9% identity in 347 aa overlap and to SW:PURR_ECOLI (EMBL:J04212) Escherichia coli purine nucleotide synthesis repressor PurR, 340 aa; fasta scores: opt: 587 z-score: 684.5 E(): 9.5e-31; 35.7% identity in 336 aa overlap and to Streptomyces coelicolor SC4G6.23; fasta scores: opt: 989 z-score: 952.6 E(): 0; 53.8% identity in 316 aa overlap. Contains matches to Pfam entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family and to Prosite entry PS00356 Bacterial regulatory proteins, lacI family signature. Contains also a possible helix-turn-helix motif at residues 10..31 (+6.13 SD) 1 3 4 14 [Reporter]SCO7593 (8M18)_PCR/4111 [Gene]SCO7593/3739 NC_003888 SC7H9.05, unknown, len: 121 aa 1 4 21 4 [Reporter]SCP1.271 (19I1)_PCR/6490 [Gene]SCP1.271/5911 NC_003888 SCP1.271, unknown, doubtful CDS, len: 53aa; 1 3 4 13 [Reporter]SCO6436 (9I18)_PCR/4110 [Gene]SCO6436/3738 NC_003888 SC9B5.03, probable tRNA synthetase, len: 506 aa; similar to many eg. SW:SYM_METTH methionyl-tRNA synthetase from Methanobacterium thermoautotrophicum (651 aa) fasta scores; opt: 610, z-score: 560.9, E(): 5.9e-24, (28.9% identity in 402 aa overlap). Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. 1 4 21 3 [Reporter]SCP1.85c (20E1)_PCR/6489 [Gene]SCP1.85c/5910 NC_003888 SCP1.85c, possible secreted protein, len: 439aa; contains possible cleavable N-terminal signal sequence. 1 3 4 12 [Reporter]SCO2458 (10E18)_PCR/4109 [Gene]SCO2458/3737 NC_003888 SCC24.29c, hypothetical protein, len: 172 aa; similar to TR:Q9X8S3 (EMBL:AL049754) Streptomyces coelicolor hypothetical 20.1 kD protein SCH10.34c, 185 aa; fasta scores: opt: 412 z-score: 505.6 E(): 1e-20; 40.7% identity in 172 aa overlap 1 3 4 11 [Reporter]SCO4362 (11A18)_PCR/4108 [Gene]SCO4362/3736 NC_003888 SCD19.17, possible two component system sensor kinase, len: 403 aa; similar to TR:O70000 (EMBL:AL022374) Streptomyces coelicolor putative two-component sensor SC5B8.14, 384 aa; fasta scores: opt: 580 z-score: 600.9 E(): 5.7e-26; 36.8% identity in 353 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 4 10 [Reporter]SCO3289 (12M14)_PCR/4107 [Gene]SCO3289/3735 NC_003888 SCE15.06, possible large membrane protein, len: 536 aa; contains a possible membrane spanning hydrophobic region 1 3 4 9 [Reporter]SCO3691 (13I14)_PCR/4106 [Gene]SCO3691/3734 NC_003888 SCH35.33c, possible regulatory protein, len: 422 aa; regions of similarity to many egs. TR:O86807 (EMBL:AL031031) putative regulatory protein from Streptomyces coelicolor (916 aa) fasta scores; opt: 541, z-score: 629.7, E(): 9.6e-28, (35.5% identity in 349 aa overlap) SW:RSBU_BACSU sigma factor associated regulatory protein from Bacillus subtilis (335 aa) fasta scores; opt: 214, z-score: 253.9, E(): 8.2e-07, (26.8% identity in 228 aa overlap). Contains probable coiled-coil region at approx. 150-165aa. 1 3 4 8 [Reporter]SCO1538 (14E14)_PCR/4105 [Gene]SCO1538/3733 NC_003888 SCL2.28c, probable transport system membrane protein, len: 328 aa; similar to SW:YESP_BACSU (EMBL:Z99107) Bacillus subtilis probable ABC transporter permease protein YesP, 309 aa; fasta scores: opt: 1013 z-score: 1146.0 E(): 0; 48.8% identity in 301 aa overlap and to TR:CAB59596 (EMBL:AL132662) Streptomyces coelicolor probable sugar transporter inner membrane protein SCF11.19, 316 aa; fasta scores: opt: 608 z-score: 690.8 E(): 4.4e-31; 32.8% identity in 320 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 4 1 9 14 [Reporter]SCO7715 (9D4)_PCR/1701 [Gene]SCO7715/1559 NC_003888 SC8D11.06, hypothetical protein, len: 267 aa; similar to TR:Q9RK84 (EMBL:AL132662) Streptomyces coelicolor hypothetical 29.4 kDa protein SCF11.05, 271 aa; fasta scores: opt: 918 z-score: 1092.7 E(): 0; 55.9% identity in 254 aa overlap 1 3 4 7 [Reporter]SCO1447 (15A14)_PCR/4104 [Gene]SCO1447/3732 NC_003888 SCL6.04c, possible ROK-family transcriptional regulatory protein, len: 399 aa; similar to TR:CAB71832 (EMBL:AL138662) Streptomyces coelicolor putative ROK-family regulatory protein SC8E4A.27, 409 aa; fasta scores: opt: 938 z-score: 965.1 E(): 0; 42.5% identity in 398 aa overlap and to SW:XYLR_ANATH (EMBL:Z69782) Anaerocellum thermophilum xylose repressor XylR, 399 aa; fasta scores: opt: 327 z-score: 341.8 E(): 1.3e-1; 22.2% identity in 397 aa overlap. Contains Pfam match to entry PF00480 ROK, ROK family and possible helix-turn-helix motif at residues 26..47 (+2.84 SD) 4 1 9 13 [Reporter]SCO6473 (9P24)_PCR/1700 [Gene]SCO6473/1558 NC_003888 SC9C7.09c, ccr, crotonyl CoA reductase, len: 447aa; similar to many, very similar to EMBL:U37135 ccr, crotonyl CoA reductase from Streptomyces collinus (447 aa) fasta scores; opt: 2819, z-score: 3635.0, E(): 0, (92.6% identity in 447 aa overlap). Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases. 1 3 4 6 [Reporter]SCO3340 (16M10)_PCR/4103 [Gene]SCO3340/3731 NC_003888 SCE7.07c, unknown, len: 400 aa; similar to TR:O53392 (EMBL:AL021841) hypothetical protein from Mycobacterium tuberculosis (243 aa) fasta scores; opt: 424, z-score: 497.4, E(): 2.2e-20, (35.8% identity in 226 aa overlap). 4 1 9 12 [Reporter]SCO2693 (10L24)_PCR/1699 [Gene]SCO2693/1557 NC_003888 SCC61A.14, possible oxygenase (putative secreted protein), len: 333 aa; similar to others from Streptomyces spp. e.g. TR:Q05582 (EMBL:L06214) clavaminate synthase 2 from Streptomyces clavuligerus (325 aa) fasta scores; opt: 582, z-score: 633.2, E(): 8.2e-28, 37.5% identity in 317 aa overlap and TR:Q9Z4Z5 (EMBL:AL035707) putative oxygenase from Streptomyces coelicolor (333 aa) opt: 1205, z-score: 1302.5, E(): 0, 59.4% identity in 320 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 4 5 [Reporter]SCO1773 (17I10)_PCR/4102 [Gene]SCO1773/3730 NC_003888 SCI51.13c, probable L-alanine dehydrogenase, len: 371 aa; similar to many alanine dehydrogenases e.g. SW:DHA_MYCTU (EMBL:X63069), ald, Mycobacterium tuberculosis L-alanine dehydrogenase (371 aa), fasta scores; opt: 1569 z-score: 1722.1 E(): 0, 65.8% identity in 371 aa overlap. Contains Pfam match to entry PF01262 AlaDh_PNT, Alanine dehydrogenase/pyridine nucleotide transhydrogenase and PS00836 Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 1 4 1 9 11 [Reporter]SCO4691 (11H24)_PCR/1698 [Gene]SCO4691/1556 NC_003888 SCD31.16, possible membrane protein, len: 197 aa; similar to C-terminal region of TR:Q9WX28 (EMBL:AL079345) Streptomyces coelicolor putative membrane protein SCE68.03, 441 aa; fasta scores: opt: 495 z-score: 618.7 E(): 5e-27; 41.5% identity in 205 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 9 10 [Reporter]SCO3506 (12D24)_PCR/1697 [Gene]SCO3506/1555 NC_003888 SCE134.07, possible lacI-family transcriptional regulator, len: 353 aa; similar to many e.g. SW:MALR_STRCO maltose operon transcriptional repressor MalR from Streptomyces coelicolor (344 aa) fasta scores; opt: 440, z-score: 508.9, E(): 4.9e-21, (32.4% identity in 352 aa overlap). Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family. 4 1 9 9 [Reporter]SCO0400 (13P20)_PCR/1696 [Gene]SCO0400/1554 NC_003888 SCF62.26, possible epimerase, len: 178 aa. Similar to many including: Streptomyces griseus SW:STRM_STRGR (EMBL; X62567) dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13) (DTDP-4-keto-6-deoxyglucose 3,5-epimerase) (DTDP-l-rhamnose synthetase) (200 aa), fasta scores opt: 340 z-score:0.0 E(): 0.0 37.78% identity in 180 aa overlap and Leptospira borgpetersenii TR:Q9ZGK0 (EMBL; AF078135) RmlC (186 aa), fasta scores pt: 432 z-score: 524.9 E(): 7.2e-22 42.3% identity in 175 aa overlap. Contains a Pfam match to entry PF00908 dTDP_sugar_isom, dTDP-4-dehydrorhamnose 3,5-epimerase. 4 1 9 8 [Reporter]SCO0807 (14L20)_PCR/1695 [Gene]SCO0807/1553 NC_003888 SCF43.18c, unknown, len: 107 aa. Highly similar to several proteins of unknown function e.g. Escherichia coli SW:YIIL_ECOLI(EMBL:L19201) hypothetical 12.3 KD protein YiiL (104 aa), fasta scores opt: 174 z-score: 248.9 E(): 1.8e-06 31.1% identity in 106 aa overlap and Bacillus subtilis TR:O05263(EMBL:Z93938) hypothetical 12.6 KD protein YulD (104 aa), fasta scores opt: 254 z-score: 355.8 E(): 1.9e-12 43.4% identity in 106 aa overlap. 4 1 9 7 [Reporter]SCO5149 (15H20)_PCR/1694 [Gene]SCO5149/1552 NC_003888 SCP8.12, possible protease, len: 542 aa; C-terminal region similar to TR:O53896 (EMBL:AL021999) Mycobacterium tuberculosis putative serine protease MTV044.11, 464 aa; fasta scores: opt: 803 z-score: 614.5 E(): 9.2e-27; 39.4% identity in 469 aa overlap and to TR:AAF61294 (EMBL:AF155705) Lactococcus lactis serine protease HtrA, 408 aa; fasta scores: opt: 536 z-score: 415.8 E(): 1.1e-15; 34.8% identity in 310 aa overlap. Contains Pfam matches to entries PF00089 trypsin, Trypsin and PF00595 PDZ, PDZ domain (Also known as DHR or GLGF) 4 1 9 6 [Reporter]SCO3636 (16D20)_PCR/1693 [Gene]SCO3636/1551 NC_003888 SCH10.14c, probable cytochrome P-450 hydroxylase, len: 411 aa; similar to many e.g. SW:CPXJ_SACER (EMBL:X60379), EryF, Saccharopolyspora erythraea 6-deoxyerythronolide B hydroxylase (404 aa), fasta scores; opt: 920 z-score: 1001.6 E(): 0, 39.2% identity in 406 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450, score 174.40, E-value 1.9e-48 and PS00086 Cytochrome P450 cysteine heme-iron ligand signature 4 1 9 5 [Reporter]SCO1197 (17P16)_PCR/1692 [Gene]SCO1197/1550 NC_003888 SCG11A.28c, possible acyl-CoA dehydrogenase, len:362 aa; low similarity to TR:AAD44195 (EMBL:AF142581) Streptomyces coelicolor acyl-CoA dehydrogenase AcdH, 386 aa; fasta scores: opt: 266 z-score: 294.1 E(): 5.2e-09; 28.3% identity in 392 aa overlap and to SW:ACDS_CLOAB (EMBL:U17110) Clostridium acetobutylicum acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2) (ScaD) (butyryl-CoA dehydrogenase) Bcd, 379 aa; fasta scores: opt: 231 z-score: 256.5 E(): 6.5e-07; 26.5% identity in 378 aa overlap. Contains two matches to Pfam entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase 1 4 21 2 [Reporter]SCO5071 (20M21)_PCR/6488 [Gene]SCO5071/5909 NC_003888 SCBAC20F6.14c, ORFA hydroxylacyl-CoA dehydrogenase, len: 146 aa; identical to previously sequenced TR:Q53925 (EMBL:X62373) Streptomyces coelicolor hydroxylacyl-CoA dehydrogenase ORFA, 146 aa 1 4 20 22 [Reporter]SCO1332 (1M17)_PCR/6486 [Gene]SCO1332/5908 NC_003888 2SCG61.14c, unknown, len: 290 aa; similar to TR:Q9Z9I2 (EMBL:AB024056) unknown protein from Streptomyces fradiae (306 aa) fasta scores; opt: 705, z-score: 786.1, E(): 0, 45.9% identity in 259 aa overlap and TR:CAB92673 (EMBL:AL356832) hypothetical protein from Streptomyces coelicolor (342 aa) fasta scores; opt: 577, z-score: 644.3, E(): 2.1e-28, 43.6% identity in 280 aa overlap 1 4 20 21 [Reporter]SCO3025 (2I17)_PCR/6485 [Gene]SCO3025/5907 NC_003888 SCE34.06c, manA, mannose-6-phosphate isomerase, len: 383 aa; similar to SW:MANA_ECOLI (EMBL:M15380) Escherichia coli mannose-6-phosphate isomerase (EC 5.3.1.8) ManA, 391 aa; fasta scores: opt: 912 z-score: 1055.7 E(): 0; 40.9% identity in 391 aa overlap. Contains Pfam match to entry PF01238 PMI_typeI, Phosphomannose isomerase type I and match to Prosite entry S00965 Phosphomannose isomerase type I signature 1 1 4 20 20 [Reporter]SCO7641 (3E17)_PCR/6484 [Gene]SCO7641/5906 NC_003888 SC10F4.14, possible dehydrogenase, len: 398 aa; similar to many other Streptomyces coelicolor putative dehydrogenases, e.g. TR:Q9RD04 (EMBL:AL133422) putative dehydrogenase SCM1.40c, 296 aa; fasta scores: opt: 352 z-score: 383.2 E(): 8.2e-14; 33.5% identity in 260 aa overlap 1 4 20 18 [Reporter]SCO7668 (5M13)_PCR/6482 [Gene]SCO7668/5905 NC_003888 SC4C2.03, conserved hypothetical protein, len: 159 aa; similar to TR:O06195 (EMBL:Z95387) Mycobacterium tuberculosis hypothetical 24.2 kDa protein MTCYA10.15c, 225 aa; fasta scores: opt: 235 z-score: 263.9 E(): 3.6e-07; 43.7% identity in 103 aa overlap 1 4 20 17 [Reporter]SCO6738 (6I13)_PCR/6481 [Gene]SCO6738/5904 NC_003888 SC5F2A.21, possible carboxypeptidase (putative secreted protein), len: 432 aa; weak similarity to many e.g. SW:CBPS_STRCP zinc carboxypeptidase from Streptomyces capreolus (434 aa) fasta scores; opt: 153, z-score: 178.1, E(): 0.013, (23.5% identity in 136 aa overlap). Contains possible N-terminal region signal peptide sequence 1 4 20 16 [Reporter]SCO5644 (7E13)_PCR/6480 [Gene]SCO5644/5903 NC_003888 SC6A9.23c, unknown, len: 208 aa; this ORF has atypically low GC bias in FramePlot 1 4 20 15 [Reporter]SCO5762 (8A13)_PCR/6479 [Gene]SCO5762/5902 NC_003888 SC4H8.01, unknown, partial CDS, len >29 aa,SC7C7.17, possible araC family transcriptional regulatory protein, len: 288 aa; some similarity to members of the araC family e.g. ADA_SALTY ADA regulatory protein (352 aa), fasta scores; opt: 161 z-score: 197.6 E(): 0.0009, 31.0% identity in 129 aa overlap. Overlaps and extends CDS from neighboring cosmid SC4H8.01. Contains probable helix-turn-helix motif at aa 189-210 (Score 1161, +3.14 SD) and Pfam match to entry HTH_2 PF00165, Bacterial regulatory helix-turn-helix proteins, araC family, score 74.61 1 3 4 4 [Reporter]SCO3551 (18E10)_PCR/4101 [Gene]SCO3551/3729 NC_003888 SCH5.14c, possible membrane spanning protein, len: 230aa; This CDS has been designated based on Hidden-Markoff Model and Frameplot predictions, lies in a region of high %GC DNA and contains a potential membrane spanning hydrophobic region. Glycine rich N-terminal. 1 4 20 14 [Reporter]SCO6995 (9M9)_PCR/6478 [Gene]SCO6995/5901 NC_003888 SC8F11.21, possible protease, len: 477 aa. Weakly similar in parts to a number or serine proteases e.g. Thermoactinomyces sp. TR:Q56365(EMBL:U31759) thermostable alkaline protease (384 aa), fast scores opt: 220 z-score: 245.7 E(): 3e-06 26.2% identity in 263 aa overlap. Contains Prosite hits to PS00136 Serine proteases, subtilase family, aspartic acid active site,PS00137 Serine proteases, subtilase family, histidine active site and PS00138 Serine proteases, subtilase family, serine active site. 1 3 4 3 [Reporter]SCO4058 (19A10)_PCR/4100 [Gene]SCO4058/3728 NC_003888 2SCD60.24, unknown, len: 167 aa 1 4 20 13 [Reporter]SCO2522 (10I9)_PCR/6477 [Gene]SCO2522/5900 NC_003888 SCC121.25c, unknown, len: 317 aa 1 3 4 2 [Reporter]SCO1872 (20I6)_PCR/4099 [Gene]SCO1872/3727 NC_003888 SCI39.19, probable IclR-family transcriptional regulator, len: 257 aa; similar to SW:ICLR_ECOLI (EMBL:M31761) Escherichia coli acetate operon repressor IclR, 274 aa; fasta scores: opt: 413 Z-score: 491.6 bits: 98.6 E(): 8.7e-20;31.855% identity in 248 aa overlap and to TR:Q9RDE7 (EMBL:AL136503) Streptomyces coelicolor putative IclR-family transcriptional regulator SCC77.11, 265aa; fasta scores: opt: 766 Z-score: 904.5 bits: 175.0 E(): 8.6e-43; 52.846% identity in 246 aa overlap. Contains Pfam match to entry PF01614 IclR, Bacterial transcriptional regulator and possible helix-turn-helix motif at residues 32..53 (+3.00 SD) 1 3 3 22 [Reporter]SCO4540 (1I2)_PCR/4097 [Gene]SCO4540/3726 NC_003888 2SCD4.11c, unknown, len: 80 aa. Low G+C content (56.25%) 1 3 3 21 [Reporter]SCO0732 (2E2)_PCR/4096 [Gene]SCO0732/3725 NC_003888 3SC5B7.10, probable secreted protease, len: 454 aa; similar to SW:PRTD_STRGR (EMBL:L29019) Streptomyces griseus serine protease D precursor (EC 3.4.21.-) SprD, 392 aa; fasta scores: opt: 964 z-score: 909.8 E(): 0; 45.4% identity in 366 aa overlap. Contains Pfam match to entry PF00089 trypsin, Trypsin and match to Prosite entry PS00134 Serine proteases, trypsin family, histidine active site and PS00135 Serine proteases, trypsin family, serine active site. Also contains possible N-terminal region signal peptide sequence 1 3 3 20 [Reporter]SCO4608 (3A2)_PCR/4095 [Gene]SCO4608/3724 NC_003888 SCD39.08, nuoN2, NADH dehydrogenase subunit, len: 515 aa; similar to SW:NUON_ECOLI (EMBL:X68301) Escherichia coli NADH dehydrogenase I chain N (EC 1.6.5.3) NuoN, 425 aa; fasta scores: opt: 601 z-score: 679.6 E(): 2.2e-30; 31.6% identity in 418 aa overlap and to TR:Q9XAR7 (EMBL:AL078618) Streptomyces coelicolor SCD16A.08c, NuoN, NADH dehydrogenase subunit, 552 aa; fasta scores: opt: 346 z-score: 334.0 E(): 5.7e-13; 32.0% identity in 563 aa overlap. Contains Pfam match to entry PF00361 oxidored_q1, NADH-Ubiquinone/plastoquinone (complex I), various chains and match to Prosite entry PS01023 PTR2 family proton/oligopeptide symporters signature 2. Also contains possible hydrophobic membrane spanning regions 1 3 3 19 [Reporter]SCO1404 (3M22)_PCR/4094 [Gene]SCO1404/3723 NC_003888 SC1A8A.24c, unknown, len: 1001aa; weakly similar to many other large proteins of undefined function eg. TR:Q9Z5A4 (EMBL:AL035478) putative secreted protein from Streptomyces coelicolor (1156 aa) fasta scores; opt: 210, z-score: 222.7, E(): 5.7e-05, 24.7% identity in 636 aa overlap. 4 1 9 4 [Reporter]SCO6081 (18L16)_PCR/1691 [Gene]SCO6081/1549 NC_003888 SCBAC1A6.05c, probable alpha amylase, len: 805 aa; similar to TR:Q53237 (EMBL:D78001) Rhizobium sp. maltooligosyl trehalose synthase TreY, 772 aa; fasta scores: opt: 1007 z-score: 1085.4 E(): 0; 42.8% identity in 797 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase 1 3 3 18 [Reporter]SCO6232 (4I22)_PCR/4093 [Gene]SCO6232/3722 NC_003888 SC2H4.14, probable beta-mannosidase, len: 820 aa; similar to several eukaryotic beta-mannosidases, e.g. MANB_HUMAN beta-mannosidase precursor (EC 3.2.1.25) (879 aa), fasta scores; opt: 679 z-score: 909.3 E(): 0, 31.4% identity in 684 aa overlap 4 1 9 3 [Reporter]SCO2238 (19H16)_PCR/1690 [Gene]SCO2238/1548 NC_003888 SCBAC17D6.05c, nadE, NAD(+) synthase (glutamine-hydrolysing), len: 613 aa; identical to TR:Q9Z4V9 (EMBL:Y17736) Streptomyces coelicolor NAD(+) synthase (glutamine-hydrolysing) (EC 6.3.5.1) (fragment) NadE, 538 aa and highly similar to SW:NADE_RHOCA (EMBL:X59399) Rhodobacter capsulatus glutamine-dependent NAD(+) synthetase (EC 6.3.5.1) (NAD(+) synthase [glutamine-hydrolysing]) NadE or AdgA, 552 aa; fasta scores: opt: 631 Z-score: 697.6 bits: 139.0 E(): 2.8e-31; 43.645% identity in 598 aa overlap. Contains Pfam matches to entries PF00795 CN_hydrolase, Carbon-nitrogen hydrolase and PF02540 NAD_synthase, NAD synthase and matches to Prosite entries PS00920 Nitrilases / cyanide hydratase signature 1 and PS00136 Serine proteases, subtilase family, aspartic acid active site 1 3 3 17 [Reporter]SCO7128 (5E22)_PCR/4092 [Gene]SCO7128/3721 NC_003888 SC4B10.29c, possible transmembrane efflux protein, len: 511 aa; similar to SW:AC22_STRCO (EMBL:M64683) Streptomyces coelicolor probable actinorhodin transporter actII-2, 578 aa; fasta scores: opt: 730 z-score: 787.0 E(): 0; 34.9% identity in 484 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and possible hydrophobic membrane spanning regions 4 1 9 2 [Reporter]SCO5975 (20P12)_PCR/1689 [Gene]SCO5975/1547 NC_003888 SCBAC16H6.10, arcA2, arginine deiminase, len: 418 aa: similar to many e.g. SW:Q9RJN1 (ARCA_STRCO) arginine deaminase ArcA SCF55.37 from Streptomyces coelicolor (420 aa) fasta scores; opt: 1124, Z-score: 1310.8, 43.350% identity (43.781% ungapped) in 406 aa overlap and SW:O31017 (ARCA_RHIET) arginine deaminase from Rhizobium etli (409 aa) fasta scores; opt: 1153, Z-score: 1344.7, 45.854% identity (47.000% ungapped) in 410 aa overlap. Contains Pfam match to entry PF02726 Arg_deiminase, Arginine deiminase. 1 3 3 16 [Reporter]SCO7584 (6A22)_PCR/4091 [Gene]SCO7584/3720 NC_003888 SC5F1.38c, probable transcriptional regulator, len: 190 aa; low similarity to SW:TER4_ECOLI (EMBL:X01083) Escherichia coli tetracycline repressor protein class D TetR, 217 aa; fasta scores: opt: 131 z-score: 169.5 E(): 0.066; 33.0% identity in 100 aa overlap. Contains possible helix-turn-helix motif at residues 27..48 (+4.31 SD) 4 1 8 22 [Reporter]SCO1239 (1P8)_PCR/1687 [Gene]SCO1239/1546 NC_003888 2SCG1.14, unknown, len: 102 aa 4 1 8 21 [Reporter]SCO0677 (2L8)_PCR/1686 [Gene]SCO0677/1545 NC_003888 SCF91.37, csn, secreted chitosanase (EC 3.2.1.132), len: 280 aa. Highly similar to several including: Streptomyces sp. (strain N174) SW:CHIS_STRSQ (EMBL:L07779) chitosanase precursor (EC 3.2.1.132) (278 aa), fasta scores opt: 1122 z-score: 1263.9 E():0 64.3% identity in 272 aa overlap and Streptomyces coelicolor TR:CAB52859 (EMBL:AL109849) putative chitosanase (305 aa), fasta scores opt: 954 z-score: 1075.3 E():0 63.7% identity in 234 aa overlap. Contains a Pfam match to entry PF01374 Glyco_hydro_46, Glycosyl hydrolase family 46 and a possible N-terminal signal sequence. 4 1 8 20 [Reporter]SCO4139 (3H8)_PCR/1685 [Gene]SCO4139/1544 NC_003888 SCD84.06c, pstB, phosphate ABC transport system ATP-binding protein, len: 258 aa; highly similar to TR:Q51546 (EMBL:D45195) Pseudomonas aeruginosa Phosphate transport ATP-binding protein PstB, 277 aa; fasta scores: opt: 1012 z-score: 1136.2 E(): 0; 60.6% identity in 254 aa overlap and to TR:Q50046 (EMBL:U15182) Mycobacterium leprae PhoT, 258 aa; fasta scores: opt: 1340 z-score: 1501.8 E(): 0; 74.8% identity in 258 aa overlap and to SW:PSTB_ECOLI (EMBL:X02723) Escherichia coli phosphate ABC transport ATP-binding protein PstB or PhoT, 257 aa; fasta scores: opt: 941 z-score: 1057.7 E(): 0; 55.7% identity in 255 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 4 1 8 19 [Reporter]SCO5529 (4D8)_PCR/1684 [Gene]SCO5529/1543 NC_003888 SC1C2.10, putative 2-isopropylmalate synthase, len: 534 aa; similar to e.g. LEU1_BACSU 2-isopropylmalate synthase (leuA) (EC 4.1.3.12) (A (518 aa), fasta scores; opt: 563 z-score: 585.3 E(): 2.4e-25, 28.1% identity in 523 aa overlap, and to e.g. NIFV_FRASP homocitrate synthase (EC 4.1.3.21) (401 aa), fasta scores; opt: 316 z-score: 554.5 E(): 1.2e-23, 33.0% identity in 358 aa overlap. Contains PS00815 Alpha-isopropylmalate and homocitrate synthases signature 1 and Pfam match to entry PF00682 HMGL-like, HMGL-like, score 126.90, E-value 3.7e-34 4 1 8 18 [Reporter]SCO6571 (5P4)_PCR/1683 [Gene]SCO6571/1542 NC_003888 SC3F9.06, possible DNA-binding protein, len: 332 aa; similar to TR:Q9A7T9 (EMBL:AE005838) Caulobacter crescentus hypothetical protein CC1631, 351 aa; fasta scores: opt: 552 Z-score: 641.5 E(): 4.2e-28; 34.940% identity in 332 aa overlap. Contains possible helix-turn-helix motif at residues 55 to 76 (Score 1148, +3.10 SD) 4 1 8 17 [Reporter]SCO6674 (6L4)_PCR/1682 [Gene]SCO6674/1541 NC_003888 SC5A7.24c, unknown, len: 78 aa 4 1 8 16 [Reporter]SCO7287 (7H4)_PCR/1681 [Gene]SCO7287/1540 NC_003888 SC5H1.05, hypothetical protein, len: 117 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 1 3 3 15 [Reporter]SCO4406 (7M18)_PCR/4090 [Gene]SCO4406/3719 NC_003888 SC6F11.04c, unknown, len: 21 aa 1 3 3 14 [Reporter]SCO2022 (8I18)_PCR/4089 [Gene]SCO2022/3718 NC_003888 SC7H2.36c, unknown, len: 252 aa; similar to SW:YCBR_BACSU hypothetical protein from Bacillus subtilis (243 aa) fasta scores; opt: 421, z-score: 523.5, E(): 7.8e-22, (32.8% identity in 229 aa overlap) 1 3 3 13 [Reporter]SCO7145 (9E18)_PCR/4088 [Gene]SCO7145/3717 NC_003888 SC9A4.07, possible transcriptional regulator, len: 160 aa; similar to TR:Q9X8V0 (EMBL:AL049826) Streptomyces coelicolor putative transcriptional regulator SCH24.36c, 154 aa; fasta scores: opt: 218 z-score: 278.5 E(): 4.8e-08; 36.5% identity in 115 aa overlap and to SW:MPRA_ECOLI (EMBL:X54151) Escherichia coli transcriptional repressor MprA, 176 aa; fasta scores: opt: 139 z-score: 182.0 E(): 0.011; 25.6% identity in 125 aa overlap. Contains Pfam match to entry PF00325 crp, Bacterial regulatory proteins, crp family and possible helix-turn-helix motif at residues 57..78 (+3.58 SD) 1 3 3 12 [Reporter]SCO2454 (10A18)_PCR/4087 [Gene]SCO2454/3716 NC_003888 SCC24.25, possible integral membrane protein, len: 743 aa; similar to SW:MMLC_STRCO (EMBL:AL031107) Streptomyces coelicolor putative membrane protein SC5A7.16c, 705 aa; fasta scores: opt: 765 z-score: 808.9 E(): 0; 37.9% identity in 737 aa overlap. Contains possible N-terminal region signal peptide sequence and possible hydrophobic membane spanning regions 1 3 3 11 [Reporter]SCO4500 (11M14)_PCR/4086 [Gene]SCO4500/3715 NC_003888 SCD35.07c, possible dehydratase, len: 291 aa; similar to TR:Q04846 (EMBL:X64795) Corynebacterium ammoniagenes fatty acid synthase (EC 2.3.1.85), Fas, 3104 aa, 3-hydroxydecanoyl beta, gamma-dehydratase domain (315 aa); fasta scores: opt: 155 z-score: 164.5 E(): 0.098; 26.9% identity in 264 aa overlap. Contains Pfam match to entry PF01575 MaoC_like, MaoC like domain 1 3 3 10 [Reporter]SCO3285 (12I14)_PCR/4085 [Gene]SCO3285/3714 NC_003888 SCE15.02c, large glycine/alanine rich protein, len: 1772aa; 1 3 3 9 [Reporter]SCO5018 (13E14)_PCR/4084 [Gene]SCO5018/3713 NC_003888 SCK15.20, possible integral membrane protein, len: 69 aa. Contains possible hydrophobic membrane spanning regions 4 1 8 15 [Reporter]SCO6689 (8D4)_PCR/1680 [Gene]SCO6689/1539 NC_003888 SC6G3.05, hypothetical protein, len: 588 aa; similar to TR:Q9RJY5 (EMBL:AL133210) Streptomyces coelicolor hypothetical protein SCF37.16, 667 aa; fasta scores: opt: 633 Z-score: 604.5 E(): 4.9e-26; 40.441% identity in 680 aa overlap. Probable CDS suggested by GC frameplot, positional base preference and amino acid composition 1 3 3 8 [Reporter]SCO0035 (14A14)_PCR/4083 [Gene]SCO0035/3712 NC_003888 SCJ4.16c, unknown, len: 149 aa 4 1 8 14 [Reporter]SCO6594 (8P24)_PCR/1679 [Gene]SCO6594/1538 NC_003888 SC8A6.15c, probable secreted protein, len: 602 aa; c ontains probable N-terminal signal sequence 1 3 3 7 [Reporter]SCO0758 (15M10)_PCR/4082 [Gene]SCO0758/3711 NC_003888 SCF81.17, unknown, len: 59 aa 4 1 8 13 [Reporter]SCO6469 (9L24)_PCR/1678 [Gene]SCO6469/1537 NC_003888 SC9C7.05c, possible acyl-CoA dehydrogenase, len: 401aa; similar to many from prokaryotes and eukaryotes egs. SW:ACDB_BACSU acyl-CoA dehydrogenase from Bacillus subtilis (379 aa) fasta scores; opt: 520, z-score: 1209.6, E(): 0, (38.9% identity in 386 aa overlap) and SW:ACDM_MOUSE acyl-CoA dehydrogenase from Mus musculus (mouse) (421 aa) fasta scores; opt: 484, z-score: 1178.1, E(): 0, (40.3% identity in 390 aa overlap). Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase. 1 3 3 6 [Reporter]SCO1637 (16I10)_PCR/4081 [Gene]SCO1637/3710 NC_003888 SCI41.20c, unknown, len: 317 aa. Similar to various hypothetical proteins, e.g. TR:P72264 (EMBL:Z82004) Rhodococcus erythropolis ORF10, 330 aa; fasta scores: opt: 578 z-score: 682.7 E(): 1.2e-30; 39.5% identity in 332 aa overlap 4 1 8 12 [Reporter]SCO2316 (10H24)_PCR/1677 [Gene]SCO2316/1536 NC_003888 SCC53.07c, unknown, len: 365aa; 4 1 8 11 [Reporter]SCO4686 (11D24)_PCR/1676 [Gene]SCO4686/1535 NC_003888 SCD31.11, hypothetical protein, len: 103 aa; similar to TR:O54115 (EMBL:AL021529) Streptomyces coelicolor hypothetical 14.3 kD protein SC10A5.24c, 134 aa; fasta scores: opt: 353 z-score: 464.3 E(): 2e-18; 75.3% identity in 73 aa overlap 4 1 8 10 [Reporter]SCO3525 (12P20)_PCR/1675 [Gene]SCO3525/1534 NC_003888 SCE2.06, putative membrane protein, len: 146 aa. Contains possible hydrophobic membrane spanning regions 4 1 8 9 [Reporter]SCO3886 (13L20)_PCR/1674 [Gene]SCO3886/1533 NC_003888 StH24.08, possible partitioning or sporulation protein, len: 275 aa; previously sequenced as TR:O07328 (EMBL:Y16311) Streptomyces coelicolor hypothetical protein (255 aa), but with a different stop codon position. Similar to many e.g. SW:SOJ_BACSU (EMBL:D26185), SOJ, Bacillus subtilis sporulation protein (253 aa), fasta scores; opt: 899 z-score: 1077.0 E(): 0, 56.6% identity in 251 aa overlap, TR:O05189 (EMBL:U87804), parA, Caulobacter crescentus chromosome partitioning protein (266 aa) (49.2% identity in 248 aa overlap) and TR:O53596 (EMBL:AL021426), parB, Mycobacterium tuberculosis hypothetical protein (347 aa) (64.3% identity in 249 aa overlap). Contains Pfam match to entry PF00991 ParA, ParA family ATPase, score 123.30, E-value 4.4e-33. Contains probable coiled-coil from 86 to 114 (29 residues) (Max score: 1.424, probability 0.80) 4 1 8 8 [Reporter]SCO3376 (14H20)_PCR/1673 [Gene]SCO3376/1532 NC_003888 SCE94.27c, possible acetyltransferase, len: 169aa; similar to hypothetical proteins eg. TR:O33289 (EMBL:AL008967) from Mycobacterium tuberculosis (174 aa) fasta scores; opt: 656, z-score: 842.4, E(): 0, (60.4% identity in 164 aa overlap). Also some similarity to SW:ARGA_ECOLI ArgA, amino-acid acetyltransferase from Escherichia coli (443 aa) fasta scores; opt: 192, z-score: 248.7, E(): 1.5e-06, (29.0% identity in 138 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 4 1 8 7 [Reporter]SCO0326 (15D20)_PCR/1672 [Gene]SCO0326/1531 NC_003888 SCF12.05, hypothetical protein, len: 322 aa; similar to TR:O54173 (EMBL:AL021411) hypothetical protein from Streptomyces coelicolor (382 aa) fasta scores; E(): 6.7e-12, 34.2% identity in 371 aa overlap. 4 1 8 6 [Reporter]SCO0663 (16P16)_PCR/1671 [Gene]SCO0663/1530 NC_003888 SCF91.23, possible 2-hydroxyacid-family dehydrogenase, len: 343 aa. Similar in parts to several D-3-phosphoglycerate dehydrogenases e.g. Mycobacterium tuberculosis SW:SERA_MYCTU (EMBL:AL021287) (528 aa), fasta scores opt: 450 z-score: 536.4 E(): 1.7e-22 34.1% identity in 261 aa overlap. Also similar to Streptomyces coelicolor TR:CAB55710(EMBL:AL117387) possible dehydrogenase SCF41.07 (339 aa), fasta scores opt: 927 z-score: 1106.0 E(): 0 48.6% identity in 325 aa overlap. Contains a Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases and a Prosite hit to PS00671 D-isomer specific 2-hydroxyacid dehydrogenases signature 3. 1 3 3 5 [Reporter]SCO3484 (17E10)_PCR/4080 [Gene]SCO3484/3709 NC_003888 SCE65.20c, possible secreted sugar-binding protein, len: 436 aa; similar to TR:P72397 (EMBL:Y07706) Streptomyces coelicolor putative maltose-binding protein MalE, 423 aa; fasta scores: opt: 361 z-score: 398.4 E(): 8.1e-15; 26.2% identity in 409 aa overlapand to TR:O87856 (EMBL:AL031013) Streptomyces coelicolor putative secreted sugar binding protein SC8A6.22, 438 aa; fasta scores: opt: 344 z-score: 379.6 E(): 9e-14; 23.6% identity in 365 aa overlap. Contains match to Pfam entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and match to Prosite entry correctly situated PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains possible N-terminal signal peptide sequence 1 3 3 4 [Reporter]SCO0176 (18A10)_PCR/4079 [Gene]SCO0176/3708 NC_003888 SCJ1.25, unknown, len: 131 aa. 1 3 3 3 [Reporter]SCO4006 (19M6)_PCR/4078 [Gene]SCO4006/3707 NC_003888 2SC10A7.10, probable fatty acid CoA ligase, len: 558 aa; similar to SW:ALKK_PSEOL (EMBL:X65936) Pseudomonas oleovorans medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-) AlkK, 546 aa; fasta scores: opt: 1453 z-score: 1654.3 E(): 0; 41.7% identity in 549 aa overlap. Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and match to Prosite entry PS00455 Putative AMP-binding domain signature 1 3 2 22 [Reporter]SCO5189 (1E2)_PCR/4075 [Gene]SCO5189/3706 NC_003888 2SC3B6.13, unknown, len: 133 aa 1 3 2 21 [Reporter]SCO0728 (2A2)_PCR/4074 [Gene]SCO0728/3705 NC_003888 3SC5B7.06, probable regulatory protein, len: 244 aa; similar to TR:Q9XDF0 (EMBL:AF074603) Streptomyces griseus putative transcriptional repressor NonG, 242 aa; fasta scores: opt: 885 z-score: 1073.4 E(): 0; 64.1% identity in 237 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and match to Prosite entry PS01081 Bacterial regulatory proteins, tetR family signature 1 3 2 19 [Reporter]SCO1400 (3I22)_PCR/4072 [Gene]SCO1400/3704 NC_003888 SC1A8A.20c, cvnC4, unknown, len: 131 aa; similar to others from Streptomyces coelicolor and Mycobacterium tuberculosis eg. TR:O86523 (EMBL:AL031124) hypothetical protein from Streptomyces coelicolor (132 aa) fasta scores; opt: 262, z-score: 339.3, E(): 1.8e-11, 41.9% identity in 124 aa overlap and to TR:Q9KZI7 (EMBL:AL353863) Streptomyces coelicolor hypothetical protein SCG8A.12c, 137 aa; fasta scores: opt: 480 Z-score: 582.4 E(): 8.3e-25; 59.690% identity in 129 aa overlap. Local genetic arrangement also seems to be conserved for the Streptomyces coelicolor paralogues 1 3 2 18 [Reporter]SCO6187 (4E22)_PCR/4071 [Gene]SCO6187/3703 NC_003888 SC2G5.08, possible bifunctional synthase/transferase, len: 495aa; N-terminal region similar to many eg. TR:Q48046 (EMBL:U17642) ADP-heptose synthase from Haemophilus influenzae (342 aa) fasta scores; opt: 301, z-score: 304.7,E() : 1.1e-09, (29.3% identity in 311 aa overlap). C-terminal region simlar to TR:O28854 (EMBL:AE001006) glycerol-3-phosphate cytidyl transferase from Archaeoglobus fulgidus (137 aa) fasta scores; opt: 220, z-score: 230.3,E(): 1.5e-05, (42.2 % identity in 90 aa overlap). 4 1 8 5 [Reporter]SCO0883 (17L16)_PCR/1670 [Gene]SCO0883/1529 NC_003888 SCM1.16, probable polypeptide deformylase (EC 3.5.1.31), len: 218 aa. Similar to many members of the polypeptide deformylase family e.g. Thermus aquaticus SW:DEF_THETH (EMBL:X79087) polypeptide deformylase (EC 3.5.1.31) (192 aa), fasta scores opt: 321 z-score: 384.1 E(): 4.8e-14 39.0% identity in 164 aa overlap. Contains a Pfam match to entry PF01327 Pep_deformylase, Polypeptide deformylase. 1 3 2 17 [Reporter]SCO7124 (5A22)_PCR/4070 [Gene]SCO7124/3702 NC_003888 SC4B10.25c, aceE3, pyruvate dehydrogenase E1 component, len: 895 aa; highly similar to SW:ODP1_ALCEU (EMBL:U09865) Alcaligenes eutrophus pyruvate dehydrogenase E1 component (EC 1.2.4.1) PdhA, 895 aa; fasta scores: opt: 2970 z-score: 3290.1 E(): 0; 50.2% identity in 878 aa overlap and to TR:CAB51267 (EMBL:AL096872) Streptomyces coelicolor putative pyruvate dehydrogenase E1 component AceE1 SC5F7.18, 899 aa; fasta scores: opt: 3647 z-score: 3704.8 E(): 0; 72.3% identity in 891 aa overlap 4 1 8 4 [Reporter]SCO3579 (18H16)_PCR/1669 [Gene]SCO3579/1528 NC_003888 SCH17.13c, probable transcriptional regulator, len: 112 aa; similar to many transcriptional regulators from Streptomyces e.g. TR:Q53963 (EMBL:X62287), WhiB, S.coelicolor transcriptional regulator essential for sporulation (87 aa), fasta scores; opt: 230 z-score: 307.8 E(): 8.1e-10, 50.0% identity in 64 aa overlap. Highest similarity is to TR:O69649 (EMBL:AL022121) Mycobacterium tuberculosis putative regulatory protein (100 aa) (67.9% identity in 78 aa overlap) 1 3 2 16 [Reporter]SCO7291 (6M18)_PCR/4069 [Gene]SCO7291/3701 NC_003888 SC5H1.01, probable serine/threonine protein kinase, partial CDS, len: >459 aa; similar to many eukaryotic-type e.g. SW:AFSK_STRCO (EMBL:D15062), afsK, Streptomyces coelicolor eukaryotic-type protein kinase involved in secondary metabolism (799 aa), fasta scores; opt: 261 z-score: 221.3 E(): 5.3e-05, 30.3% identity in 498 aa overlap and SW:KRCB_RAT (EMBL:D30041) Rattus norvegicus RAC-beta serine/threonine kinase (481 aa) (31.4% identity in 169 aa overlap). A hydrophobic region is present, so it is possible that the protein spans a membrane. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain, score 30.80, E-value 1.9e-07,SC5F8.01c, possible serine/threonine protein kinase, partials CDS, len:> 114 aa. Similar to many proposed Streptomyces coelicolor serine/threonine protein kinases e.g. TR:CAB82014(EMBL:AL161755) SCD63.07 (717 aa), fasta scores opt: 172 z-score: 222.0 E(): 6.8e-05 33.0% identity in 103 aa overlap. Overlaps and extends into CDS SC5H1.01 on the adjoining cosmid. 4 1 8 3 [Reporter]SCP1.171 (19D16)_PCR/1668 [Gene]SCP1.171/1527 NC_003888 SCP1.171, possible integral membrane protein, len: 297aa; similar to TR:Q9L0R6 (EMBL:AL160312) putative integral membrane protein from Streptomyces coelicolor (302 aa) fasta scores; opt: 379, z-score: 425.8, E(): 3e-16, 30.3% identity in 297 aa overlap. Contains possible membrane-spanning hydrophobic regions. 1 3 2 15 [Reporter]SCO2127 (7I18)_PCR/4068 [Gene]SCO2127/3700 NC_003888 SC6E10.21c, hypothetical protein, len: 191 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Previously sequenced as SW:YGL2_STRCO (EMBL:X65932) Streptomyces coelicolor hypothetical protein (191 aa), identical to this sequence 4 1 8 2 [Reporter]SCO5996 (20L12)_PCR/1667 [Gene]SCO5996/1526 NC_003888 SCBAC16H6.31, possible membrane protein, len: 937 aa: similar to TR:O54182 (EMBL:AL021411) hypothetical protein SC7H1.23 from Streptomyces coelicolor (913 aa) fasta scores; opt: 1128, Z-score: 1138.9, 29.418% identity (31.524% ungapped) in 928 aa overlap. C-terminal region contains 13 Pfam matches to entry PF00400 WD40, WD domain, G-beta repeat; 5 Prosite matches to PS00678 Trp-Asp (WD) repeats signature and a Prosite match to PS00626 Regulator of chromosome condensation (RCC1) signature 2 4 1 7 22 [Reporter]SCO1229 (1L8)_PCR/1665 [Gene]SCO1229/1525 NC_003888 2SCG1.04, possible reductase, len: 353 aa; similar to TR:CAB90862 (EMBL:AL355752) Streptomyces coelicolor putative reductase SC10B7.09, 366 aa; fasta scores: opt: 182 z-score: 207.8 E(): 0.00042; 31.9% identity in 295 aa overlap 4 1 7 21 [Reporter]SCO5849 (2H8)_PCR/1664 [Gene]SCO5849/1524 NC_003888 SC9B10.16, agaS, len: 385 aa; similar to AGAS_ECOLI P42907 agas protein (384 aa) (possible tagatose-6-phosphate ketose/aldose isomerase), fasta scores; opt: 960 z-score: 1350.4 E(): 0, 45.9% identity in 366 aa overlap 4 1 7 20 [Reporter]SCO5244 (3D8)_PCR/1663 [Gene]SCO5244/1523 NC_003888 2SC7G11.06c, prs1, anti-sigma factor, len: 137 aa; identical to previously sequenced TR:Q9R3X8 (EMBL:AJ249450) Streptomyces coelicolor A3(2) putative regulator of Sig1, Prs1 protein or UshX, 137 aa 4 1 7 19 [Reporter]SCO6613 (4P4)_PCR/1662 [Gene]SCO6613/1522 NC_003888 SC1F2.10, unknown, len: 135 aa; similar to hypothetical proteins from several organisms e.g. YTFH_ECOLI (156 aa), fasta scores; opt: 315 z-score: 460.2 E(): 2.2e-18, 40.5% identity in 116 aa overlap 4 1 7 18 [Reporter]SCO6567 (5L4)_PCR/1661 [Gene]SCO6567/1521 NC_003888 SC3F9.02, ABC transporter ATP binding protein, len:505 aa; similar to many sugar transport proteins e.g. ARAG_ECOLI l-arabinose transport atp-binding protein (504 aa), fasta scores; opt: 1312 z-score: 1412.8 E(): 0, 42.1% identity in 501 aa overlap andRBSA_HAEIN ribose transport atp-binding protein rbsA (493 aa), fasta scores; opt: 1160 z-score: 1216.7 E(): 0, 40.7% identity in 489 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature and Pfam match to entry ABC_tran PF00005, ABC transporters, score 197.70 4 1 7 17 [Reporter]SCO6649 (6H4)_PCR/1660 [Gene]SCO6649/1520 NC_003888 SC4G2.23, unknown, len: 224 aa 4 1 7 16 [Reporter]SCO0321 (7D4)_PCR/1659 [Gene]SCO0321/1519 NC_003888 SC5G9.30, probable carboxylesterase, len: 518 aa; similar to many eukaryotic and bacterial e.g. SW:PNBA_BACSU (EMBL:U06089), PrnbA, Bacillus subtilis 4-nitrobenzyl esterase (489 aa), fasta scores; opt: 718 z-score: 774.9 E(): 0, 36.3% identity in 479 aa overlap. Similar to TR:Q9Z545 (EMBL:AL035212) Streptomyces coelicolor probable carboxylesterase (502 aa) (41.3% identity in 521 aa overlap). Contains 2 Pfam matches to entry PF00135 COesterase, Carboxylesterases and PS00122 Carboxylesterases type-B serine active site 4 1 7 15 [Reporter]SCO2139 (7P24)_PCR/1658 [Gene]SCO2139/1518 NC_003888 SC6G10.12, possible integral membrane protein, len: 256aa; similar to TR:O69868 (EMBL:AL023702) putative integral membrane protein from Streptomyces coelicolor (383 aa) fasta scores; opt: 569, z-score: 706.4, E(): 4.8e-32, (44.6% identity in 242 aa overlap). Contains possible hydrophobic membrane sapnning regions 4 1 7 14 [Reporter]SCO6590 (8L24)_PCR/1657 [Gene]SCO6590/1517 NC_003888 SC8A6.11c, probable secreted esterase, len: 735 aa; similar to TR:Q54345 (EMBL:Z50108) esterase precursor from Streptomyces lividans (509 aa), fasta scores; opt: 553 z-sc ore: 526.3 E(): 4.5e-22, 38.2% identity in 403 aa overlap a nd to other membres of the trypsin family. Contains probabl e N-terminal signal sequence and Pfam match to entry PF0008 9 trypsin, Trypsin, score 31.60, E-value 4.4e-07 4 1 7 13 [Reporter]SCO5836 (9H24)_PCR/1656 [Gene]SCO5836/1516 NC_003888 SC9B10.03c, DNA gyrase-like protein, subunit A, len: 818 aa; similarity (strongest in N-terminus, around active site) to DNA gyrase subunit A from many organisms eg. GYRA_MYCTU Q07702 dna gyrase subunit a (ec 5.99.1.3) from Mycobacterium tuberculosis (838 aa), fasta scores; opt: 1835 z-score: 1379.9 E(): 0, 42.4% identity in 768 aa overlap; and to S. coelicolor GYRA_STRCO P35885 dna gyrase subunit a (ec 5.99.1.3) (864 aa), fasta scores; opt: 1886 z-score: 1425.4 E(): 0, 42.9% identity in 788 aa overlap. Contains probable coiled-coil region from aa 441 to 486 4 1 7 12 [Reporter]SCO2312 (10D24)_PCR/1655 [Gene]SCO2312/1515 NC_003888 SCC53.03, putative secreted protein, len: 238 aa. Contains possible N-terminal region signal peptide sequence 4 1 7 11 [Reporter]SCO4665 (11P20)_PCR/1654 [Gene]SCO4665/1514 NC_003888 SCD40A.11c, possible membrane protein, len: 209 aa. Contains possible hydrophobic membrane spanning regions 4 1 7 9 [Reporter]SCO0229 (13H20)_PCR/1652 [Gene]SCO0229/1513 NC_003888 SCJ9A.08, probable oxidoreductase (putative secreted protein), len: 282 aa; similar to many e.g. TR:CAB51136 (EMBL:Y18817) oxidoreductase of short chain from Streptomyces coelicolor (276 aa) fasta scores; opt: 615, z-score: 697.7, E(): 1.7e-31, (40.9% identity in 274 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature 4 1 7 8 [Reporter]SCO0061 (14D20)_PCR/1651 [Gene]SCO0061/1512 NC_003888 SCJ4.42c, unknown, len: 434 aa; similar to SW:YDEG_SCHPO hypothetical protein from Schizosaccharomyces pombe (fission yeast) (509 aa) fasta scores; opt: 665, z-score: 791.0, E(): 0, (37.6% identity in 423 aa overlap) and to TR:Q9KEQ9 (EMBL:AP001509) Bacillus halodurans BH0790 protein, 454 aa; fasta scores: opt: 1262 Z-score: 1468.0 bits: 280.8 E(): 3.9e-74; 45.433% identity in 416 aa overlap 4 1 7 7 [Reporter]SCO0973 (15P16)_PCR/1650 [Gene]SCO0973/1511 NC_003888 SCM11.28, probable integral membrane protein, len: 419 aa; similar to various putative integral membran proteins, e.g. TR:Q9XAN8 (EMBL:AL079355) Streptomyces coelicolor putative integral membrane protein SC4C6.02c, 317 aa; fasta scores: opt: 674 z-score: 607.8 E(): 1.8e-26; 43.6% identity in 326 aa overlap 4 1 7 6 [Reporter]SCO3780 (16L16)_PCR/1649 [Gene]SCO3780/1510 NC_003888 SCH63.27, probable sugar hydrolase, len: 556 aa; similar to SW:TREC_ECOLI (EMBL:U06195) Escherichia coli trehalose-6-phosphate hydrolase (EC 3.2.1.93) TreC, 551 aa; fasta scores: opt: 833 z-score: 927.7 E(): 0; 35.4% identity in 557 aa overlap and to TR:CAB90876 (EMBL:AL355753) Streptomyces coelicolor aglA, alpha-glucosidase SC10B7.23c, 577 aa; fasta scores: opt: 1146 z-score: 1084.7 E(): 0; 56.8% identity in 572 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase 4 1 7 5 [Reporter]SCO3724 (17H16)_PCR/1648 [Gene]SCO3724/1509 NC_003888 SCH22A.02c, hypothetical protein, len: 160 aa; similar to TR:Q9X7Q5 (EMBL:AL049587) Streptomyces coelicolor hypothetical 17.1 kD protein SC5F2A.32, 161 aa; fasta scores: opt: 256 z-score: 310.3 E(): 7.3e-10; 39.4% identity in 160 aa overlap 4 1 7 4 [Reporter]SCO0878 (18D16)_PCR/1647 [Gene]SCO0878/1508 NC_003888 SCM1.11, possible hydrolase, len: 292 aa. Highly similar to several including: Streptomyces peucetius TR:Q54809(EMBL:L40425) 10-carbomethoxy-13-deoxycarminomycin esterase (298 aa), fasta scores opt: 670 z-score: 757.9 E():0 40.3% identity in 288 aa overlap and Streptomyces purpurascens TR:Q54528(EMBL:U10405:) possible C-C hydrolase, RdmC (298 aa) fasta scores opt: 709 z-score: 801.6 E():0 41.2% identity in 289 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 4 1 7 3 [Reporter]SCP1.275 (19P12)_PCR/1646 [Gene]SCP1.275/1507 NC_003888 SCP1.275, unknown, doubtful CDS, len: 121aa; 4 1 6 22 [Reporter]SCO5261 (1H8)_PCR/1643 [Gene]SCO5261/1506 NC_003888 2SC7G11.23, probable malate oxidoreductase, len: 409 aa; similar to SW:MAOX_BACST (EMBL:M19485) Bacillus stearothermophilus NAD-dependent malic enzyme (EC 1.1.1.38), 478 aa; fasta scores: opt: 1385 z-score: 1470.9 E(): 0; 55.0% identity in 391 aa overlap. Contains 2x Pfam matches to entry PF00390 malic, Malic enzyme 4 1 6 21 [Reporter]SCO2792 (2D8)_PCR/1642 [Gene]SCO2792/1505 NC_003888 SCC105.23, adpA, araC-family transcriptional regulator, len: 398 aa; highly similar to TR:BAA86265 (EMBL:AB023785) Streptomyces griseus AdpA, 405 aa; fasta scores: opt: 2219 z-score: 2270.5 E(): 0; 84.4% identity in 410 aa overlap and to TR:Q9XA73 (EMBL:AL096822) Streptomyces coelicolor putative araC-family transcriptional regulator SCGD3.05, 334 aa; fasta scores: opt: 878 z-score: 904.2 E(): 0; 45.7% identity in 313 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family and match to Prosite entry PS00041 Bacterial regulatory proteins, araC family signature. Contains possible helix-turn-helix motif 4 1 6 20 [Reporter]SCO4707 (3P4)_PCR/1641 [Gene]SCO4707/1504 NC_003888 SCD31.32, rplV, 50S ribosomal protein L22, len: 125 aa; highly similar to SW:RL22_BACST (EMBL:X54994) Bacillus stearothermophilus 50S ribosomal protein L22, RplV, 113 aa; fasta scores: opt: 441 z-score: 566.9 E(): 3.8e-24; 64.0% identity in 114 aa overlap. Contains Pfam match to entry PF00237 Ribosomal_L22, Ribosomal protein L22 and match to Prosite entry PS00464 Ribosomal protein L22 signature 4 1 6 18 [Reporter]SCO6361 (5H4)_PCR/1639 [Gene]SCO6361/1503 NC_003888 SC3A7.29, probable integral membrane protein, len: 279 aa. Contains possible hydrophobic membrane spanning regions 4 1 6 17 [Reporter]SCO6645 (6D4)_PCR/1638 [Gene]SCO6645/1502 NC_003888 SC4G2.19, probable transport system permease protein, len: 601 aa; similar to many members of the oppBC family e.g. NIKB_ECOLI nickel transport system permease protein (314 aa), fasta scores; opt: 405 z-score: 322.1 E(): 1.1e-10, 33.4% identity in 290 aa overlap 4 1 6 16 [Reporter]SCO4859 (6P24)_PCR/1637 [Gene]SCO4859/1501 NC_003888 SC5G8.27c, unknown, len: 183 aa; similar to TR:Q9Z593 (EMBL:AL035478) hypothetical protein from Streptomyces coelicolor (191 aa) fasta scores; opt: 164, z-score: 195.7, E(): 0.0019, 32.6% identity in 187 aa overlap. 4 1 6 15 [Reporter]SCO2135 (7L24)_PCR/1636 [Gene]SCO2135/1500 NC_003888 SC6G10.08c, possible secreted protein, len: 338 aa; similar to TR:O69580 (EMBL:AL022602) putative secreted protein from Mycobacterium leprae (374 aa) fasta scores; opt: 382, z-score: 384.3, E(): 4.2e-14, (32.4% identity in 358 aa overlap). Also similar to several examples of Listeria P60 a major extracellular protein involved in the invasion of non-professional phagocytic cells eg. SW:P60_LISIV from Listeria ivanovii (524 aa) fasta scores; opt: 337, z-score: 338.3, E(): 1.5e-11, (29.1% identity in 309 aa overlap). Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family and the C-terminus, a probable membrane spanning hydrophobic region towards the N-terminus and two possible coiled coil regions. NOTE: highly similar to upstream gene, 6G10.09c, indicating a duplication event; fasta scores: opt: 1042, z-score: 843.1, E(): 0, (49.9% identity in 339 aa overlap). 2 4 22 9 [Reporter]SCO0062 (14B9)_PCR/7021 [Gene]SCO0062/6399 NC_003888 SCJ4.43c, probable LacI family transcriptional regulator, len: 367 aa; similar to many transcriptional regulators e.g. SW:GALR_ECOLI galactose operon repressor (343 aa) fasta scores; opt: 549, z-score: 636.2, E(): 4.5e-28, (32.5% identity in 332 aa overlap). Contains Pfam matches to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family and entry PF00356 lacI, Bacterial regulatory proteins, lacI family. Contains possible helix-turn-helix motif (+3.86 SD) 26-47. 2 4 22 8 [Reporter]SCO3889 (15N5)_PCR/7020 [Gene]SCO3889/6398 NC_003888 SCH24.11c, trxA, thioredoxin, len: 110 aa; previously sequenced (EMBL:AJ007313), TrxA. Contains Pfam match to entry PF00085 thiored, Thioredoxin, score 140.90, E-value 3.6e-41 and PS00194 Thioredoxin family active site 2 4 22 7 [Reporter]SCO1057 (16J5)_PCR/7019 [Gene]SCO1057/6397 NC_003888 SCG22.03, possible sugar transport integral membrane protein, len: 321 aa; similar to TR:Q9RK91 (EMBL:AL117322) Streptomyces coelicolor putative binding-protein-dependent transport protein SCF1.13, 317 aa; fasta scores: opt: 681 z-score: 798.6 E(): 0; 35.0% identity in 317 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 2 4 22 6 [Reporter]SCO3758 (17F5)_PCR/7018 [Gene]SCO3758/6396 NC_003888 SCH63.05c, possible fatty acid desaturase, len: 350 aa; similar to TR:Q9X8W4 (EMBL:AL078610) Streptomyces coelicolor putative delta fatty acid desaturase SCH35.42c, 345 aa; fasta scores: opt: 1041 z-score: 1260.0 E(): 0; 50.3% identity in 332 aa overlap and to TR:Q9SWQ9 (EMBL:AF139720) Euglena gracilis delta 8 fatty acid desaturase Efd1, 419 aa; fasta scores: opt: 421 z-score: 510.9 E(): 6.1e-21; 27.3% identity in 333 aa overlap. Contains possible hydrophobic membrane spanning regions 2 4 22 5 [Reporter]SCO3738 (18B5)_PCR/7017 [Gene]SCO3738/6395 NC_003888 SCH22A.16c, possible integral membrane protein, len: 189 aa. Contains possible hydrophobic membrane spanning regions 2 4 22 4 [Reporter]SCP1.256c (19N1)_PCR/7016 [Gene]SCP1.256c/6394 NC_003888 SCP1.256c, doubtful CDS, unknown, len: 54aa; 2 4 22 3 [Reporter]SCO4010 (20J1)_PCR/7015 [Gene]SCO4010/6393 NC_003888 2SC10A7.14c, possible secreted protein, len: 155 aa; similar to C-terminal region of SW:SSI1_STRCI (PIR:PC1265) Streptomyces cacaoi subtilisin inhibitor-like protein-1 (Sil-1), 110 aa; fasta scores: opt: 179 z-score: 206.6 E(): 0.00053; 36.1% identity in 97 aa overlap. Contains possible N-terminal region signal peptide sequence 2 4 21 22 [Reporter]SCO1136 (1B21)_PCR/7012 [Gene]SCO1136/6392 NC_003888 2SCG38.29c, probable two component system response regulator, len: 263 aa; similar to SW:DCUR_ECOLI (EMBL:U14003) Escherichia coli transcriptional regulatory protein DcuR, 239 aa; fasta scores: opt: 421 z-score: 469.7 E(): 1e-18; 35.0% identity in 226 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain and possible helix-turn-helix motif at residues 201.222 (+3.87 SD) 2 4 21 21 [Reporter]SCO4827 (2N17)_PCR/7011 [Gene]SCO4827/6391 NC_003888 SC2A6.12, mdh, malate dehydrogenase, len: 329 aa; similar to SW:MDH_THEFL (EMBL:J02598) Thermus aquaticus malate dehydrogenase (EC 1.1.1.37) Mdh, 327 aa; fasta scores: opt: 1398 z-score: 1643.5 E(): 0; 64.5% identity in 327 aa overlap. Contains Pfam match to entry PF00056 ldh, lactate/malate dehydrogenase and match to Prosite entry PS00068 Malate dehydrogenase active site signature 2 4 21 20 [Reporter]SCO7699 (3J17)_PCR/7010 [Gene]SCO7699/6390 NC_003888 SC1A4.07, possible nucleotide-binding protein, len: 471 aa; highly similar to TR:Q54255 (EMBL:L76204) Streptomyces griseus sporulation-specific protein p3, 470 aa; fasta scores: opt: 2382 z-score: 2757.2 E(): 0; 75.8% identity in 472 aa overlap and to TR:CAC05970 (EMBL:AL391763) Streptomyces coelicolor putative nucleotide binding protein 2SC7G11.11, 468 aa; fasta scores: opt: 1972 z-score: 2283.0 E(): 0; 63.5% identity in 466 aa overlap. Contains Pfam match to entry PF00027 cNMP_binding, Cyclic nucleotide-binding domain 2 4 21 19 [Reporter]SCO5600 (4F17)_PCR/7009 [Gene]SCO5600/6389 NC_003888 SC2E1.17, unknown, len: 172 aa; similar to C-terminus of M. tuberculosis hypothetical protein TR:O06205 (EMBL:Z95387) MTCY1A10.24 (351 aa), fasta scores; opt: 345 z-score: 375.2 E(): 1.2e-13, 44.7% identity in 161 aa overlap. Contains PS00893 mutT domain signature and Pfam match to entry mutT PF00293, Bacterial mutT protein, score 37.78 2 4 21 18 [Reporter]SCO2404 (5B17)_PCR/7008 [Gene]SCO2404/6388 NC_003888 SC4A7.32, probable sugar-binding receptor, len: 369 aa; similar to SW:CVE2_AGRTU (EMBL:X97456) Agrobacterium tumefaciens multiple sugar-binding periplasmic receptor chve precursor, ChvE, 354 aa; fasta scores: opt: 1391 z-score: 1497.2 E(): 0; 60.3% identity in 350 aa overlap. Contains possible signal peptide sequence, Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family and Prosite match correctly situated PS00013 Prokaryotic membrane lipoprotein lipid attachment site 2 4 21 17 [Reporter]SCO6743 (6N13)_PCR/7007 [Gene]SCO6743/6387 NC_003888 SC5F2A.26c, possible transcriptional accessory protein, len: 780aa; similar to SW:TEX_BORPE Tex, transcriptional accessory protein (exact function not known) from Bordetella pertussis (791 aa) fasta scores; opt: 2905, z-score: 2938.8, E(): 0, (59.5% identity in 781 aa overlap). Also similar to hypothetical proteins eg. SW:YHGF_ECOLI from Escherichia coli fasta scores; opt: 2888, z-score: 2922.0, E(): 0, (61.2% identity in 744 aa overlap). Contains Pfam match to entry PF00575 S1, S1 RNA binding domain. 2 4 21 16 [Reporter]SCO1425 (7J13)_PCR/7006 [Gene]SCO1425/6386 NC_003888 SC6D7.14, possible AsnC-family transcriptional regulatory protein, len: 165 aa. Highly similar to several proteins of unknown function and to the Lrp (leucine-responsive regulatory protein) global regulatory proteins e.g. Escherichia coli SW:LRP_ECOLI(EMBL:M35869) leucine-responsive regulatory protein (163 aa), fasta scores opt: 283 z-score: 348.1 E(): 5.2e-12 37.1% identity in 140 aa overlap and Mycobacterium tuberculosis TR:P96896(EMBL:Z92771) hypothetical 16.5 KD protein (150 aa), fasta scores opt: 503 z-score: 608.4 E(): 1.6e-26 50.3% identity in 143 aa overlap. Contains a Pfam match to entry PF01037 ASNC_trans_reg, AsnC family and a Prosite hit to PS00519 Bacterial regulatory proteins, asnC family signature. Contains a putative helix-turn-helix motif between residues 38..59 (+4.53 SD). 2 4 21 15 [Reporter]SCO5210 (8F13)_PCR/7005 [Gene]SCO5210/6385 NC_003888 SC7E4.07c, possible molecular chaperone, len: 107 aa; similar to SW:SUGE_ECOLI (EMBL:X69949) Escherichia coli SugE protein, 105 aa; fasta scores: opt: 341 z-score: 449.1 E(): 1.5e-17; 44.7% identity in 103 aa overlap and to TR:CAB88839 (EMBL:AL353832) Streptomyces coelicolor sugE, SCE6.35c, 106 aa; fasta scores: opt: 323 z-score: 386.5 E(): 6e-16; 43.0% identity in 107 aa overlap. Contains Pfam match to entry PF00893 DUF7, Integral membrane protein and possible hydrophobic membrane spanning regions 2 4 21 14 [Reporter]SCO5411 (9B13)_PCR/7004 [Gene]SCO5411/6384 NC_003888 SC8F4.15c, possible integrase/recombinase, len: 468aa; similar to many eg. TR:Q38184 (EMBL:X85213) integrase from Bacteriophage TP901-1 (485 aa) fasta scores; opt: 281, z-score: 318.4, E(): 2.5e-10, 26.9% identity in 428 aa overlap. Contains Pfam match to entry PF00239 recombinase, Site-specific recombinases and 2x tta Leu codon, possible target for bldA regulation. 2 4 21 13 [Reporter]SCO2527 (10N9)_PCR/7003 [Gene]SCO2527/6383 NC_003888 SCC121.30c, unknown, len: 231 aa 2 4 21 12 [Reporter]SCO4403 (11J9)_PCR/7002 [Gene]SCO4403/6382 NC_003888 SC6F11.01, probable formyltetrahydrofolate deformylase (fragment), len: >136 aa; highly similar to SW:PURU_ECOLI (EMBL:L20251) Escherichia coli formyltetrahydrofolate deformylase (EC 3.5.1.10) PurU, 280 aa; fasta scores: opt: 421 z-score: 514.5 E(): 3.7e-21; 47.0% identity in 134 aa overlap. Contains Pfam match to entry PF00551 formyl_transf, Formyl transferase,SCD10.35, probable formyltetrahydrofolate deformylase (fragment), len: >201 aa; similar to SW:PURU_CORSP (EMBL:U23955) Corynebacterium sp. formyltetrahydrofolate deformylase (EC 3.5.1.10) PurU, 286 aa; fasta scores: opt: 601 z-score: 759.7 E(): 0; 49.7% identity in 185 aa overlap. Contains Pfam match to entry PF00551 formyl_transf, Formyl transferase 2 4 21 11 [Reporter]SCO4478 (12F9)_PCR/7001 [Gene]SCO4478/6381 NC_003888 SCD65.21, possible transferase, len: 203 aa; similar to TR:Q9RWR3 (EMBL:AE001918) Deinococcus radiodurans cytidine/deoxycytidylate deaminase/nudix/methyltransferase domains protein DR603, 548 aa; fasta scores: opt: 447 z-score: 514.2 E(): 3.6e-21; 44.1% identity in 186 aa overlap. Contains match to Prosite entry PS00847 MCM family signature 2 4 21 10 [Reporter]SCO3239 (13B9)_PCR/7000 [Gene]SCO3239/6380 NC_003888 SCE29.08c, unknown, len: 289 aa; similar to TR:O69952 (EMBL:AL023862) hypothetical protein from Streptomyces coelicolor (282 aa) fasta scores; opt: 1391, z-score: 1679.9, E(): 0, (72.3% identity in 285 aa overlap). 2 4 21 9 [Reporter]SCO1583 (14N5)_PCR/6999 [Gene]SCO1583/6379 NC_003888 SCI35.05c, probable secreted arabinosidase, len: 824 aa; similar to e.g. TR:Q59218 (EMBL:U15178) arabinosidase (EC 3.2.1.55) (Bacteroides ovatus) (660 aa), fasta scores; opt: 1204 z-score: 1030.7 E(): 0, 41.4% identity in 601 aa overlap 2 4 21 8 [Reporter]SCO1525 (15J5)_PCR/6998 [Gene]SCO1525/6378 NC_003888 SCL2.15c, possible sugar transferase, len: 387 aa; similar to TR:O28546 (EMBL:AE000983) Archaeoglobus fulgidus galactosyltransferase, 356 aa; fasta scores: opt: 422 z-score: 488.7 E(): 7.9e-20; 26.9% identity in 375 aa overlap and to TR:O06204 (EMBL:Z95387) Mycobacterium tuberculosis hypothetical 40.4 Kd protein MTCY1A10.23, 378 aa; fasta scores: opt: 1216 z-score: 1400.3 E(): 0; 52.7% identity in 376 aa overlap. Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1 2 4 21 7 [Reporter]SCO0070 (16F5)_PCR/6997 [Gene]SCO0070/6377 NC_003888 SCJ4.51, unknown, possibly involved in sugar metabolism, len: 22 aa; similar over region to many hypothetical sugar metabolism proteins e.g. SW:VIPB_SALTI polysaccharide biosynthesis protein from Salmonella enterica (348 aa) fasta scores; opt: 220, z-score: 251.9, E(): 1.1e-06, (31.8% identity in 170 aa overlap). Note overlap with downstream gene. 2 4 21 6 [Reporter]SCO3345 (17B5)_PCR/6996 [Gene]SCO3345/6376 NC_003888 SCE7.12c, ilvD, dihydroxy acid dehydratase, len: 617aa; previously sequenced therefore almost (conflict of 3 amino acids) identical to TR:O69198 (EMBL:AF068843) ilvD, dihydroxy acid dehydratase from Streptomyces coelicolor (617 aa) fasta scores; opt: 4019, z-score: 4363.1, E(): 0, (99.2% identity in 617 aa overlap). Also similar to SW:ILVD_ECOLI ilvD, dihydroxy acid dehydratase from Escherichia coli (605 aa) fasta scores; opt: 2644, z-score: 2871.0, E(): 0, (66.5% identity in 606 aa overlap). Contains Pfam match to entry PF00920 ILVD_EDD, Dehydratase family and Prosite matches to PS00887 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 2 and PS00886 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1. 2 4 21 5 [Reporter]SCO0204 (18N1)_PCR/6995 [Gene]SCO0204/6375 NC_003888 SCJ12.16c, probable luxR family two-component response regulator, len: 233 aa. Highly similar to many including: several Streptomyces coelicolor two-component response regulators such as SW:CAB46941 (EMBL; AL096822) putative response regulator SCGD3.19 (218 aa), fasta scores opt: 865 z-score: 987.7 E(): 0 64.7% identity in 215 aa overlap. In addition to Pseudomonas aeruginosa TR:O54039 (EMBL; Y15252) nitrate/nitrite regulatory protein NarL (216 aa), fasta scores opt: 458 z-score: 528.5 E(): 4.4e-22 38.6% identity in 210 aa overlap. Contains a Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, Pfam match to entry PF00072 response_reg, Response regulator receiver domain and a PS00622 Bacterial regulatory proteins, luxR family signature. Contains a possible helix-turn-helix motif between aa's 182..203. 2 4 21 4 [Reporter]SCO6318 (19J1)_PCR/6994 [Gene]SCO6318/6374 NC_003888 SCIF3.20c, conserved hypothetical protein, len: 190 aa; similar to SW:TERD_ALCSP (EMBL:M20238) Alcaligenes sp. tellurium resistance protein TerD, 192 aa; fasta scores: opt: 204 z-score: 247.1 E(): 3.2e-06; 28.3% identity in 173 aa overlap and to TR:CAA18342 (EMBL:AL022268) Streptomyces coelicolor hypothetical protein SC4H2.27c, 202 aa; fasta scores: opt: 685 z-score: 748.3 E(): 0; 56.1% identity in 180 aa overlap. Contains Pfam match to entry PF02342 TerD 2 4 21 3 [Reporter]SCO7467 (20F1)_PCR/6993 [Gene]SCO7467/6373 NC_003888 SCBAC14E8.07, unknown, len: 375 aa 2 4 21 2 [Reporter]SCO7506 (20N21)_PCR/6992 [Gene]SCO7506/6372 NC_003888 SCBAC17A6.39, possible hydrolase, len: 606 aa; similar to many e.g. TR:Q9AHJ8 (EMBL:AF305888) beta-glucuronidase from Lactobacillus gasseri (598 aa) fasta scores: opt: 437, Z-score: 491.4, 25.054% identity (27.913% ungapped) in 459 aa overlap. Contains Pfam match to entry PF02837 Glyco_hydro_2_N, Glycosyl hydrolases family 2, sugar binding domain; Pfam match to entry PF00703 Glyco_hydro_2, Glycosyl hydrolases family 2, immunoglobulin-like beta-sandwich domain and Pfam match to entry PF02836 Glyco_hydro_2_C, Glycosyl hydrolases family 2, TIM barrel domain. 2 3 4 4 [Reporter]SCO0636 (18F10)_PCR/4605 [Gene]SCO0636/4199 NC_003888 SCF56.20, probable ABC transporter ATP-binding protein, len: 544 aa; similar to TR:Q9X7M6 (EMBL:AL049587) Streptomyces coelicolor putative ABC transporter SC5F2A.03c, 544 aa; fasta scores: opt: 1673 z-score: 1706.2 E(): 0; 52.2% identity in 542 aa overlap and to SW:MSRA_STAEP (EMBL:X52085) Staphylococcus epidermidis erythromycin resistance ATP-binding protein MsrA, 488 aa; fasta scores: opt: 351 z-score: 362.8 E(): 7.9e-13; 28.6% identity in 514 aa overlap. Contains two Pfam matches to entry PF00005 ABC_tran, ABC transporter and two matches to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 4 20 22 [Reporter]SCO1333 (1N17)_PCR/6990 [Gene]SCO1333/6371 NC_003888 2SCG61.15, possible integral membrane protein, len: 322 aa; similar to TR:CAC44603 (EMBL:AL596162) Streptomyces coelicolor putative integral membrane protein SCBAC16H6.24c, 294 aa; fasta scores: opt: 900 Z-score: 871.5 E(): 6.5e-41; 52.597% identity in 308 aa overlap. Contains possible membrane-spanning hydrophobic regions 2 3 4 3 [Reporter]SCP1.186 (19B10)_PCR/4604 [Gene]SCP1.186/4198 NC_003888 SCP1.186, conserved hypothetical protein, len: 282aa; similar to other hypothetical proteins from streptomycetes eg. SCE6.06 (TR:Q9KZT2 EMBL:AL353832) from Streptomyces coelicolor (281 aa) fasta scores; opt: 882, z-score: 1083.5, E(): 0, 47.9% identity in 280 aa overlap. Note possible alternative downstream translational start site. 2 4 20 21 [Reporter]SCO3028 (2J17)_PCR/6989 [Gene]SCO3028/6370 NC_003888 SCE34.09c, manB, phosphomannomutase, len: 454 aa; similar to SW:XANA_XANCP (EMBL:M83231) Xanthomonas campestris phosphoglucomutase/phosphomannomutase [includes: phosphoglucomutase(EC 5.4.2.2) (glucose phosphomutase) (PGM); phosphomannomutase (EC 5.4.2.8) (PMM)] XanA, 448 aa; fasta scores: opt: 1096 z-score: 1203.0 E(): 0; 42.0% identity in 443 aa overlap. Contains Pfam match to entry PF00408 PGM_PMM, Phosphoglucomutase/phosphomannomutase 2 3 4 2 [Reporter]SCP1.248c (20J6)_PCR/4603 [Gene]SCP1.248c/4197 NC_003888 SCP1.248c, unknown, len: 79aa; 2 3 3 22 [Reporter]SCO4541 (1J2)_PCR/4601 [Gene]SCO4541/4196 NC_003888 2SCD4.12c, possible membrane protein, len: 526 aa; N-terminal region identical to previously sequenced TR:Q9XD98 (EMBL:AF106004) Streptomyces coelicolor hypothetical 27.5 kDa protein, 249 aa and to TR:CAB82065 (EMBL:AL161803) Streptomyces coelicolor putative integral membrane protein SCD31.22, 604 aa; fasta scores: opt: 1299 z-score: 1315.4 E(): 0; 57.8% identity in 339 aa overlap. Contains possible coiled-coil region at approx. residues 108..198 and possible hydrophobic membrane spanning region 2 3 3 21 [Reporter]SCO0733 (2F2)_PCR/4600 [Gene]SCO0733/4195 NC_003888 3SC5B7.11c, hypothetical protein, len: 61 aa; similar to TR:Q9K3Z9 (EMBL:AL359949) Streptomyces coelicolor hypothetical 6.5 kDa protein 2SCG61.16, 59 aa; fasta scores: opt: 196 z-score: 304.8 E(): 1.9e-09; 59.3% identity in 54 aa overlap 2 3 3 20 [Reporter]SCO4647 (3B2)_PCR/4599 [Gene]SCO4647/4194 NC_003888 SCD82.18, nusG, transcription antitermination protein, len: 300aa; identical to previously sequenced SW:NUSG_STRCO (EMBL:D17465) Streptomyces coelicolor transcription antitermination protein NusG. Contains match to Prosite entry PS01014 Transcription termination factor nusG signature. Contains also possible coiled-coil region at aprox. residues 56..75 2 3 3 19 [Reporter]SCO1405 (3N22)_PCR/4598 [Gene]SCO1405/4193 NC_003888 SC1A8A.25c, possible heat shock protein (hsp90-family), len: 615aa; similar to many eg. TR:O43642 (EMBL:AF043254) heat shock protein 75 from Homo sapiens (Human) (649 aa) fasta scores; opt: 364, z-score: 416.0, E(): 9.7e-16, 25.5% identity in 639 aa overlap. Contains two Pfam matches to entry PF00183 HSP90, Hsp90 protein. 2 3 3 18 [Reporter]SCO6233 (4J22)_PCR/4597 [Gene]SCO6233/4192 NC_003888 SC2H4.15, probable transcriptional regulator, len: 342 aa; similar to many members of the lacI family e.g. CCPA_BACME glucose-resistance amylase regulator (Bacillus megaterium) (332 aa), fasta scores; opt: 448 z-score: 548.6 E(): 2.6e-23, 29.8% identity in 336 aa overlap. Contains helix-turn-helix motif from aa 9-30 (Score 1778, +5.24 SD) and Pfam matches to entry PF00356 lacI, Bacterial regulatory proteins, lacI family, score 30.40, E-value 4.7e-07 and to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family, score 44.00, E-value 3.4e-09 2 3 3 17 [Reporter]SCO7130 (5F22)_PCR/4596 [Gene]SCO7130/4191 NC_003888 SC4B10.31, hypothetical protein, len: 262 aa; similar to TR:O53404 (EMBL:AL021897) Mycobacterium tuberculosis hypothetical 28.9 kDa protein MTV017.09, 254 aa; fasta scores: opt: 820 z-score: 1018.9 E(): 0; 49.0% identity in 249 aa overlap 1 1 16 3 [Reporter]SCO4979 (19A24)_PCR/332 [Gene]SCO4979/299 NC_003888 2SCK36.02, probable phosphoenolpyruvate carboxykinase, len: 609 aa; similar to SW:PPCK_CHLLI (EMBL:S56812) Chlorobium limicola phosphoenolpyruvate carboxykinase [GTP] (EC 4.1.1.32) PckA, 646 aa; fasta scores: opt: 2400 Z-score: 2689.0 bits: 507.7 E(): 3.6e-142; 58.361% identity in 598 aa overlap. Contains Pfam match to entry PF00821 PEPCK, Phosphoenolpyruvate carboxykinase and match to Prosite entry PS00505 Phosphoenolpyruvate carboxykinase (GTP) signature 2 3 3 16 [Reporter]SCO7585 (6B22)_PCR/4595 [Gene]SCO7585/4190 NC_003888 SC5F1.39c, probable merR-family transcriptional regulator, len: 132 aa; similar to SW:SOXR_PSEAE (EMBL:X95517) Pseudomonas aeruginosa SoxR protein, 156 aa; fasta scores: opt: 205 z-score: 264.7 E(): 3.3e-07; 36.8% identity in 117 aa overlap. Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and match to Prosite entry PS00552 Bacterial regulatory proteins, merR family signature and possible helix-turn-helix at residues 12..33 (+4.37 SD) 1 1 16 2 [Reporter]SCO2825 (20I20)_PCR/331 [Gene]SCO2825/298 NC_003888 SCBAC17F8.16, possible amidase, len: 467 aa: similar to many e.g. TR:Q9ZHK8 (EMBL:AF058285) pyrazinamidase from Mycobacterium smegmatis (468 aa) fasta scores; opt: 767, Z-score: 783.4, 34.573% identity (35.666% ungapped) in 457 aa overlap and SW:Q9Z580 (GATA_STRCO) glutamyl-tRNA amidotransferase subunit A SC8D9.11 from Streptomyces coelicolor (497 aa) fasta scores; opt: 628, Z-score: 642.1, 33.128% identity (35.698% ungapped) in 486 aa overlap. Contains Pfam match to entry PF01425 Amidase, Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00571 Amidases signature 1 1 15 22 [Reporter]SCO1225 (1I16)_PCR/329 [Gene]SCO1225/297 NC_003888 2SCG58.25, probable osmoprotectant transporter, len: 504 aa; similar to SW:PROP_ECOLI (EMBL:M83089) Escherichia coli proline/betaine transporter ProP, 500 aa; fasta scores: opt: 1534 z-score: 1728.8 E(): 0; 44.7% identity in 465 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and matches to Prosite entries PS00217 Sugar transport proteins signature 2 and PS00216 Sugar transport proteins signature 1. Also contains possible hydrophobic membrane spanning regions 1 1 15 21 [Reporter]SCO5456 (2E16)_PCR/328 [Gene]SCO5456/296 NC_003888 SC3D11.13c, glgX2, possible glycosyl hydrolase (putative secreted protein), len: 782 aa; similar to many eg. TR:Q9X947 (EMBL:AJ001206) putative glycogen debranching enzyme from Streptomyces coelicolor (715 aa) fasta scores; opt: 2630, z-score: 2693.4, E(): 0, 58.5% identity in 725 aa overlap and SW:P15067 (GLGX_ECOLI) glycogen operon protein from Escherichia coli (657 aa) fasta scores; opt: 1608, z-score: 1647.7, E(): 0, 47.2% identity in 669 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase. Contains possible N-terminal region signal peptide sequence 1 1 15 20 [Reporter]SCO7800 (3A16)_PCR/327 [Gene]SCO7800/295 NC_003888 SC10B8A.10, unknown, doubtful CDS, len: 71 aa 1 1 15 19 [Reporter]SCO7031 (4M12)_PCR/326 [Gene]SCO7031/294 NC_003888 SC1H10.20, beta-D-xylosidase, len: 797 aa. Highly similar to Streptomyces lividans TR:Q9ZI15 (EMBL:AF043654) beta-D-xylosidase, BxlA (861 aa), fasta scores opt: 4473 z-score: 4774.4 E(): 0 89.2% identity in 789 aa overlap. Also highly similar to Streptomyces coelicolor TR:CAB55650(EMBL:AL117385) putative beta-xylosidase, SC5G9.02 (796 aa), fasta scores opt: 4215 z-score: 4499.4 E(): 0 79.1% identity in 788 aa overlap. Contains a Pfam matches to entries PF00933 Glyco_hydro_3, Glycosyl hydrolase family 3 N terminal domain and Pfam match to entry PF01915 Glyco_hydro_3_C, Glycosyl hydrolase family 3 C terminal domain. 1 1 15 17 [Reporter]SCO5809 (6E12)_PCR/324 [Gene]SCO5809/293 NC_003888 SC4H2.30, possible hydrolase, len: 301 aa; some similarity to several e.g. DRAG_RHORU P14300 adp-ribosylglycohydrolase (294 aa), fasta scores; opt: 92 z-score: 265.8 E(): 1.4e-07, 25.3% identity in 296 aa overlap 1 1 15 16 [Reporter]SCO5703 (7A12)_PCR/323 [Gene]SCO5703/292 NC_003888 SC5H4.27, hypothetical protein, len: 177 aa; similar to TR:O05817 (EMBL:Z95207) Mycobacterium tuberculosis hypothetical 19.5 kD protein MTCY24A1.15, 183 aa; fasta scores: opt: 288 z-score: 348.5 E(): 6e-12; 37.2% identity in 148 aa overlap 1 1 15 15 [Reporter]SCO2484 (8M8)_PCR/322 [Gene]SCO2484/291 NC_003888 SC7A8.23, possible secreted protein, len: 428 aa; similar to TR:O86542 (EMBL:AL031350) Streptomyces coelicolor putative secreted protein SC1F2.05, 552 aa; fasta scores: opt: 440 z-score: 473.6 E(): 5.5e-19; 32.3% identity in 406 aa overlap. Contains possible N-terminal region signal peptide sequence 1 1 15 14 [Reporter]SCO6981 (9I8)_PCR/321 [Gene]SCO6981/290 NC_003888 SC8F11.07, probable ABC transport protein, ATP-binding component, len: 330 aa. Highly similar to many including: Bacillus subtilis SW:RBSA_BACSU(EMBL:Z25798) ribose transport ATP-binding protein, RbsA (453 aa), fasta scores opt: 489 z-score: 537.5 E(): 1.6e-22 31.0% identity in 239 aa overlap and Streptomyces coelicolor TR:CAB60178(EMBL:AL132824) probable ABC sugar transport ATP binding protein, SCAH10.24(263 aa), fasta scores opt: 1171 z-score: 1281.9 E():0 68.0% identity in 256 aa overlap. Contains a Prosite hits to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also contains Pfam match to entry PF00005 ABC_tran, ABC transporter. 2 4 20 20 [Reporter]SCO7642 (3F17)_PCR/6988 [Gene]SCO7642/6369 NC_003888 SC10F4.15, conserved hypothetical protein, len: 121 aa; similar to TR:O31535 (EMBL:Z99107) Bacillus subtilis hypothetical protein YetH, 120 aa; fasta scores: opt: 176 z-score: 223.7 E(): 6.3e-05; 29.8% identity in 121 aa overlap 2 4 20 19 [Reporter]SCO5589 (4B17)_PCR/6987 [Gene]SCO5589/6368 NC_003888 SC2E1.06c, unknown, len: 295 aa 2 4 20 18 [Reporter]SCO7669 (5N13)_PCR/6986 [Gene]SCO7669/6367 NC_003888 SC4C2.04c, possible oxidoreductase, len: 343 aa; similar to TR:O06538 (EMBL:Z95584) Mycobacterium tuberculosis hypothetical 36.3 kDa protein MTCI65.05c, 338 aa; fasta scores: opt: 1020 z-score: 1087.7 E(): 0; 50.7% identity in 339 aa overlap. Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase 2 4 20 17 [Reporter]SCO6739 (6J13)_PCR/6985 [Gene]SCO6739/6366 NC_003888 SC5F2A.22, probable BCCT family transporter, len: 578aa; similar to many eg. SW:OPUD_BACSU glycine betaine transporter, OpuD from Bacillus subtilis (512 aa) fasta scores; opt: 1414, z-score: 1650.4, E(): 0, (43.9% identity in 492 aa overlap). Contains Prosite match to PS01303 BCCT family of transporters signature. Also contains several probable membrane spanning hydrophobic regions. 2 4 20 16 [Reporter]SCO5643 (7F13)_PCR/6984 [Gene]SCO5643/6365 NC_003888 SC6A9.24, questionable ORF, len: 175 aa; this ORF has atypically low GC bias in FramePlot 2 4 20 15 [Reporter]SCO5205 (8B13)_PCR/6983 [Gene]SCO5205/6364 NC_003888 SC7E4.02, hypothetical protein, len: 607 aa; similar to TR:CAB83499 (EMBL:AL162752) Neisseria meningitidis hypothetical 51.6 kD protein NMA0184, 468 aa; fasta scores: opt: 268 z-score: 307.6 E(): 1.2e-09; 29.5% identity in 528 aa overlap 2 4 20 14 [Reporter]SCO6996 (9N9)_PCR/6982 [Gene]SCO6996/6363 NC_003888 SC8F11.22, possible RNA polymerase sigma factor, len: 191 aa. Weakly similar to Streptomyces coelicolor sigma factor TR:CAB76082 (EMBL:AL157953) putative RNA polymerase sigma factor, SCL11.20C (166 aa), fasta scores opt: 206 z-score: 259.9 E(): 4.8e-07 30.4% identity in 161 aa overlap. 2 4 20 13 [Reporter]SCO2523 (10J9)_PCR/6981 [Gene]SCO2523/6362 NC_003888 SCC121.26c, unknown, len: 305 aa 2 3 3 15 [Reporter]SCO4407 (7N18)_PCR/4594 [Gene]SCO4407/4189 NC_003888 SC6F11.05, hypothetical protein, len: 257 aa; partially similar to TR:Q9X8P9 (EMBL:AL049754) Streptomyces coelicolor hypothetical 17.1 kDa protein SCH10.10c, 152 aa; fasta scores: opt: 314 z-score: 367.2 E(): 6e-13 40.4% identity in 141 aa overlap 2 4 20 12 [Reporter]SCO2559 (11F9)_PCR/6980 [Gene]SCO2559/6361 NC_003888 SCC77.26c, possible oxygen-independent coproporphyrinogen III oxidase (EC 1.-.-.-), len: 435 aa. Highly similar to many putative coproporphyrinogen III oxidases including: Bacillus subtilis SW:HEMN_BACSU (EMBL:X91655) probable oxygen-independent coproporphyrinogen III oxidase (366 aa), fasta scores opt: 490 z-score: 555.3 E(): 1.5e-23 30.8% identity in 328 aa overlap and Mycobacterium tuberculosis SW:HEMN_MYCTU (EMBL:Z81368) probable oxygen-independent coproporphyrinogen III oxidase (375 aa), fasta scores opt: 1358 z-score: 1530.5 E():0 56.5% identity in 382 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 2 3 3 14 [Reporter]SCO7589 (8J18)_PCR/4593 [Gene]SCO7589/4188 NC_003888 SC7H9.01c, unknown (fragment), len: >158,SC5F1.43c, unknown (fragment), len: >161 aa 2 4 20 11 [Reporter]SCO4474 (12B9)_PCR/6979 [Gene]SCO4474/6360 NC_003888 SCD65.17, possible integral membrane protein, len: 574 aa; similar to TR:O06394 (EMBL:Z95558) Mycobacterium tuberculosis hypothetical 57.1 kDa protein MTCY25D10.07, 529 aa; fasta scores: opt: 1079 z-score: 1222.2 E(): 0; 37.3% identity in 539 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 3 13 [Reporter]SCO7146 (9F18)_PCR/4592 [Gene]SCO7146/4187 NC_003888 SC9A4.08, possible regulatory protein, len: 116 aa; similar to TR:Q9X8Y1 (EMBL:AL078610) Streptomyces coelicolor putative transcriptional regulator SCH35.25, 102 aa; fasta scores: opt: 144 z-score: 203.2 E(): 0.00075; 37.9% identity in 87 aa overlap. Contains Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family 2 3 3 12 [Reporter]SCO2455 (10B18)_PCR/4591 [Gene]SCO2455/4186 NC_003888 SCC24.26c, possible spermidine synthase, len: 531 aa; similar to TR:Q9X8S2 (EMBL:AL049754) Streptomyces coelicolor putative spermidine synthase SCH10.33c, 554 aa; fasta scores: opt: 1413 z-score: 1545.7 E(): 0; 47.9% identity in 528 aa overlap and C-terminal region similar to SW:SPEE_ECOLI (EMBL:J02804) Escherichia coli spermidine synthase (EC 2.5.1.16) SpeE, 287 aa; fasta scores: opt: 420 z-score: 465.4 E(): 1.7e-18; 31.4% identity in 271 aa overlap. Contains Pfam match in C-terminal region to entry PF01564 Spermine_synth, Spermine/spermidine synthase and N-terminal region contains also possible hydrophobic membrane spanning regions 2 3 3 11 [Reporter]SCO4501 (11N14)_PCR/4590 [Gene]SCO4501/4185 NC_003888 SCD35.08c, probable bifunctional protein (ketoreductase/3-oxoacyl-[acyl-carrier protein] reductase), len: 430 aa; highly similar to TR:O53665 (EMBL:AL021929) Mycobacterium tuberculosis 3-oxoacyl-[acyl-carrier protein] reductase MTV034.08c, 454 aa; fasta scores: opt: 1227 z-score: 1186.5 E(): 0; 50.8% identity in 455 aa overlap, N-terminal region similar to TR:O86480 (EMBL:AJ007932) Streptomyces argillaceus ketoreductase MtmTII, 253 aa; blastp results: Identities = 54/204 (26%), Positives = 87/204 (42%) and C-terminal region to SW:FABG_ECOLI (EMBL:M84991) Escherichia coli 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) (3-ketoacyl-acyl carrier protein reductase) FabG, 244 aa; fasta scores: opt: 538 z-score: 529.0 E(): 4.9e-22; 41.0% identity in 249 aa overlap. Contains Pfam matches to entries PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus,PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 2 3 3 10 [Reporter]SCO3286 (12J14)_PCR/4589 [Gene]SCO3286/4184 NC_003888 SCE15.03, putative secreted protein, len: 677 aa; weak similarity to SW:YQIK_ECOLI hypothetical protein from Escherichia coli (553 aa) fasta scores; opt: 542, z-score: 501.2, E(): 1.3e-20, (27.0% identity in 504 aa overlap). Contains possible N-terminal region signal peptide sequence also contains a highly hidrophilic central part of the protein 2 3 3 9 [Reporter]SCO3416 (13F14)_PCR/4588 [Gene]SCO3416/4183 NC_003888 SCE9.23, gad, probable glutamate decarboxylase, len: 475 aa; similar to many e.g. SW:DCEA_ECOLI (EMBL:M84024), gadA, Escherichia coli glutamate decarboxylase alpha (466 aa), fasta scores; opt: 1433 z-score: 1665.2 E(): 0, 50.0% identity in 426 aa overlap. Contains Pfam match to entry PF00282 pyridoxal_deC, Pyridoxal-dependent decarboxylase conserved domain, score 276.70, E-value 1.1e-79 2 3 3 8 [Reporter]SCO4872 (14B14)_PCR/4587 [Gene]SCO4872/4182 NC_003888 SCK20.13c, unknown, len: 338 aa 2 3 3 7 [Reporter]SCO0193 (15N10)_PCR/4586 [Gene]SCO0193/4181 NC_003888 SCJ12.05c, possible DNA-binding regulator, len:334 aa. Some similarity to Myxococcus xanthus TR:Q50900 (EMBL:Z21955) regulatory protein (299 aa), fasta scores opt: 121 z-score: 141.6 E(): 1.6 32.4% identity in 281 aa overlap and Myxococcus xanthus TR:Q50899 (EMBL:Z21955) regulatory protein (288 aa), fasta scores opt: 118 z-score: 138.5 E() 2.4 26.9% identity in 279 aa overlap. Contains a possible helix-turn-helix motif between aa's 23..44. 1 1 15 13 [Reporter]SCO7355 (10E8)_PCR/320 [Gene]SCO7355/289 NC_003888 SC9H11.09c, unknown, len: 213 aa; similar to the N-terminal region of many ATP-dependent DNA ligases, e.g. TR:Q98J69 (EMBL:AP002998) Rhizobium loti (Mesorhizobium loti) probable ATP-dependent DNA ligase MLL2077, 833 aa; fasta scores:opt: 539 Z-score: 543.3 E(): 1.3e-22; 53.216% identity in 171 aa overlap 2 3 3 6 [Reporter]SCO0745 (16J10)_PCR/4585 [Gene]SCO0745/4180 NC_003888 SCF81.04c, possible tetR-family transcriptional regulator, len: 219 aa; similar to various Streptomyces transcriptional regulators, e.g. TR:Q9ZBK0 (EMBL:AL035161) Streptomyces coelicolor putative transcriptional regulator SC9C7.10c, 272 aa; fasta scores: opt: 189 z-score: 243.3 E(): 3.5e-06; 29.8% identity in 218 aa overlap. Contains match to Pfam entry PF00440 tetR, Bacterial regulatory proteins, tetR family 1 1 15 12 [Reporter]SCO2543 (11A8)_PCR/319 [Gene]SCO2543/288 NC_003888 SCC77.10c, possible dihydrodipicolinate synthase. len: 322 aa. Similar to many dihydrodipicolinate synthases including: Bacillus subtilis SW:DAPA_BACSU (EMBL:L08471) dihydrodipicolinate synthase (EC 4.2.1.52) (290 aa), fasta scores opt: 341 z-score: 396.9 E(): 1e-14 26.8% identity in 284 aa overlap and Streptomyces coelicolor TR:Q9X9X7 (EMBL:AL096743) putative dihydropicolinate synthase SCI7.13C (316 aa), fasta scores opt: 927 z-score: 1065.7 E():0 53.1% identity in 294 aa overlap. Contains a Pfam match to entry PF00701 DHDPS. 1 1 15 11 [Reporter]SCO3939 (12M4)_PCR/318 [Gene]SCO3939/287 NC_003888 SCD78.06, unknown, len: 482 aa; similar to hypothetical proteins in tellurite resistance operon from Serratia marcescens and Alcaligenes sp. e.g. TR:Q52359 EMBL:L38824 terF gene from Serratia marcesens Plasmid R478 (413 aa), fasta scores; opt: 377 z-score: 637.8 E(): 2.8e-28, 33.4% identity in 479 aa overlap. Also simlar in C-terminus to YCBR_BACSU hypothetical 28.0 kd protein in pcp 5'region (243 aa), fasta scores; opt: 301 z-score: 372.2 E(): 1.8e-13, 36.6% identity in 238 aa overlap. Contains Pro-rich region around aa 200 1 1 15 10 [Reporter]SCO3436 (13I4)_PCR/317 [Gene]SCO3436/286 NC_003888 SCE36.03, possible fatty acid CoA ligase, len: 517 aa; similar to many eg. TR:O86483 (EMBL:AJ007932) acyl CoA ligase from Streptomyces argillaceus mithramycin biosynthesis cluster (514 aa) fasta scores; opt: 625, z-score: 678.0, E(): 1.9e-30, (32.7% identity in 513 aa overlap). Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and Prosite match to PS00455 Putative AMP-binding domain signature. 1 1 15 9 [Reporter]SCO0184 (14E4)_PCR/316 [Gene]SCO0184/285 NC_003888 SCJ1.33 unknown, len: 604 aa. Highly similar to Synechocystis sp. (strain PCC6803) TR:P74080 (EMBL;D90912) hypothetical 58.1 KD protein (504 aa), fasta scores opt: 931 z-score: 996.4 E(): 0 38.8% identity in 479 aa overlap and Methanobacterium thermoautotrophicum TR:O27804 (EMBL;AE000932) conserved protein MTH1776 (491 aa), fasta scores opt: 572 z-score: 613.5 E(): 8.1e-27 31.7% identity in 483 aa overlap. Also shares a low level of similarity with Streptomyces coelicolor TR:CAB48919 (EMBL;AL096837) putative deoxyribodipyrimidine photolyase SCF43A.32 (458 aa), fasta scores opt: 176 z-score: 191.4 E(): 0.0026 31.1% identity in 219 aa overlap. 1 1 15 8 [Reporter]SCO1548 (15A4)_PCR/315 [Gene]SCO1548/284 NC_003888 SCL11.04c, unknown, len: 86 aa 1 1 15 7 [Reporter]SCO0501 (15M24)_PCR/314 [Gene]SCO0501/283 NC_003888 SCF34.20c, putative secreted protein, len: 650 aa; unknown function, shows very weak similarity to SW:Y0D8_MYCTU (EMBL:Z78020) Mycobacterium tuberculosis hypothetical protein (628 aa), fasta scores; opt: 233 z-score: 232.7 E(): 1.3e-05, 23.6% identity in 512 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 1 15 6 [Reporter]SCO1445 (16I24)_PCR/313 [Gene]SCO1445/282 NC_003888 SCL6.02, hypothetical protein, len: aa; similar to various hypothetical proteins, e.g. TR:CAB52958 (EMBL:AL109950) Streptomyces coelicolor hypothetical 25.2 kD protein, SCJ4.24c, 242 aa; fasta scores: opt: 424 z-score: 482.7 E(): 1.8e-19; 39.9% identity in 218 aa overlap and to TR:Q9EWF2 (EMBL:AL451182) Streptomyces coelicolor hypothetical protein SCK13.32c, 263 aa; fasta scores: opt: 711 Z-score: 779.3 E(): 8.9e-36; 49.407% identity in 253 aa overlap 1 1 15 5 [Reporter]SCO1685 (17E24)_PCR/312 [Gene]SCO1685/281 NC_003888 SCI30A.06, possible integral membrane protein, len: 297aa; similar to many egs. TR:O33234 (EMBL:Z98209) hypothetical protein from Mycobacterium tuberculosis (301 aa) fasta scores; opt: 356, z-score: 407.0, E(): 2.4e-15, (31.5% identity in 289 aa overlap) and TR:O86668 (EMBL:AL031182) putative integral membrane protein from Streptomyces coelicolor (299 aa) fasta scores; opt: 272, z-score: 312.9, E(): 4.2e-10, (31.5% identity in 279 aa overlap). 1 1 15 4 [Reporter]SCO5050 (18A24)_PCR/311 [Gene]SCO5050/280 NC_003888 SCK7.23, possible nucleotide-sugar dehydrogenase, len: 443 aa; similar to TR:CAB82012 (EMBL:AL161755) Streptomyces coelicolor putative nucleotide-sugar dehydrogenase SCD63.05c, 405 aa; fasta scores: opt: 736 z-score: 812.9 E(): 0; 36.2% identity in 423 aa overlap. Contains Pfam match to entry PF00984 UDPG_MGDP_dh, UDP-glucose/GDP-mannose dehydrogenase family 2 4 20 10 [Reporter]SCO3266 (13N5)_PCR/6978 [Gene]SCO3266/6359 NC_003888 SCE39.16c, unknown, len: 67aa; 2 4 20 9 [Reporter]SCO3311 (14J5)_PCR/6977 [Gene]SCO3311/6358 NC_003888 SCE68.09c, hemB, delta-aminolevulinic acid dehydratase, len: 330 aa; previously sequenced as SW:HEM2_STRCO (EMBL:U19249) (330 aa). Contains probable coiled-coil from 218 to 252 (35 residues) (Max score: 1.530, probability 0.97). Contains Pfam match to entry PF00490 ALAD, Delta-aminolevulinic acid dehydratase and PS00169 Delta-aminolevulinic acid dehydratase active site 2 4 20 8 [Reporter]SCO0418 (15F5)_PCR/6976 [Gene]SCO0418/6357 NC_003888 SCF51.17, DNA-binding protein, len: 215 aa; similar to TR:CAB44405 (EMBL:AL078610) Streptomyces coelicolor putative DNA-binding protein, SCH35.22c 215 aa; fasta scores: opt: 206 z-score: 233.8 E(): 1.2e-05; 30.9% identity in 220 aa overlap and to TR:CAB45035 (EMBL:AL078635) Amycolatopsis orientalis hypothetical protein CZA382.14c, 202 aa; fasta scores: opt: 305 z-score: 340.6 E(): 1.3e-11; 34.0% identity in 212 aa overlap 2 4 20 7 [Reporter]SCO3790 (16B5)_PCR/6975 [Gene]SCO3790/6356 NC_003888 SCH63.37c, conserved hypothetical protein, len: 693 aa; similar to TR:AAF95947 (EMBL:AE004347) Vibrio cholerae hypothetical 68.9 kDa protein VCA0033, 624 aa; fasta scores: opt: 936 z-score: 990.2 E(): 0; 39.4% identity in 670 aa overlap 2 4 20 6 [Reporter]SCO3409 (17N1)_PCR/6974 [Gene]SCO3409/6355 NC_003888 SCE9.16, ppa, probable inorganic pyrophosphatase, len: 163 aa; similar to many e.g. SW:IPYR_SULAC (EMBL:X81842), ppa, Sulfolobus acidocaldarius inorganic pyrophosphatase (173 aa), fasta scores; opt: 483 z-score: 617.4 E(): 4.6e-27, 46.4% identity in 166 aa overlap. Contains Pfam match to entry PF00719 Pyrophosphatase, Inorganic pyrophosphatase, score 163.70, E-value 3e-45 and PS00387 Inorganic pyrophosphatase signature 2 4 20 5 [Reporter]SCO1722 (18J1)_PCR/6973 [Gene]SCO1722/6354 NC_003888 SCI11.11c, possible membrane protein, len: 250 aa; unknown function, similar, except at the N-terminus, to SW:Y24K_STRGR (EMBL:X15060) Streptomyces griseus hypothetical protein (238 aa), fasta scores; opt: 274 z-score: 308.4 E(): 7.6e-10, 33.0% identity in 176 aa overlap. Also similar to TR:O53729 (EMBL:AL021932) mycobacterium tuberculosis hypothetical protein (232 aa) (31.1% identity in 241 aa overlap). Contains a hydrophobic, possible membrane-spanning region 2 4 20 4 [Reporter]SCO6309 (19F1)_PCR/6972 [Gene]SCO6309/6353 NC_003888 SCIF3.11, probable lysR-family transcriptional regulator, len: 323 aa; similar to SW:NOD2_RHITR (EMBL:L01272) Rhizobium tropici nodulation protein D2 NodD2, 314 aa; fasta scores: opt: 534 z-score: 620.6 E(): 5e-27; 34.7% identity in 314 aa overlap and to TR:CAC05670 (EMBL:Y19177) Streptomyces antibioticus transcriptional regulator, 308 aa; fasta scores: opt: 1769 z-score: 2038.8 E(): 0; 87.3% identity in 308 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and PF01046 NodD_C_term, NodD transcription activator carboxyl terminal region and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Also contains possible helix-turn-helix motif at residues 21..42 (+3.27 SD) 2 4 20 3 [Reporter]SCO4895 (20B1)_PCR/6971 [Gene]SCO4895/6352 NC_003888 2SCK8.21c, possible ECF sigma factor, len: 334 aa; similar to TR:O07426 (EMBL:Z97050) Mycobacterium tuberculosis RNA polymerase sigma subunit MTCI28.22c, 370 aa; fasta scores: opt: 818 z-score: 941.2 E(): 0; 44.7% identity in 318 aa overlap and to TR:Q9K3H7 (EMBL:AL390188) Streptomyces coelicolor putative ECF-sigma factor 2SCG18.10c, 295 aa; fasta scores: opt: 219 z-score: 257.6 E(): 8.8e-07; 31.8% identity in 314 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) and possible helix-turn-helix motif at residues 159..180 (+4.07 SD) 2 3 3 5 [Reporter]SCO3667 (17F10)_PCR/4584 [Gene]SCO3667/4179 NC_003888 SCH44.07c, probable secreted solute-binding protein, len: 372 aa; similar to TR:O50503 (EMBL:AL009199) Streptomyces coelicolor possible solute-binding protein SC7B7.06, 371 aa; fasta scores: opt: 1441 z-score: 1539.7 E(): 0; 60.6% identity in 376 aa overlap and to SW:XYLF_ECOLI (EMBL:U00039) Escherichia coli D-xylose-binding periplasmic protein precursor XylF, 330 aa; fasta scores: opt: 559 z-score: 602.9 E(): 4.1e-26; 34.3% identity in 318 aa overlap. Contains Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide sequence 2 4 20 2 [Reporter]SCO7489 (20J21)_PCR/6970 [Gene]SCO7489/6351 NC_003888 SCBAC17A6.22c, possible binding protein dependent transport lipoprotein, len: 467aa; weakly similar to many eg. SW:P76042 (YCJN_ECOLI) putative ABC transporter from Escherichia coli (430 aa) fasta scores: opt: 346, Z-score: 385.6, 26.009% identity (29.668% ungapped) in 446 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site 2 3 3 4 [Reporter]SCO0870 (18B10)_PCR/4583 [Gene]SCO0870/4178 NC_003888 SCM1.03c, probable two-component system response regulator, len: 149 aa. Highly similar to the response regulator receiver domains of putative two-component regulatory proteins including: Synechocystis sp. (strain PCC 6803) TR:P72781(EMBL:D90900) regulatory components of sensory transduction system (282 aa), fasta scores opt: 229 z-score: 290.6 E(): 7.7e-09 31.8% identity in 129 aa overlap and Methanobacterium thermoautotrophicum TR:O26545(EMBL:AE000828) sensory transduction regulatory protein (145 aa), fasta scores opt: 422 z-score: 531.6 E(): 2.9e-22 47.2% identity in 142 aa overlap. Contains a Pfam match to entry PF00072 response_reg, Response regulator receiver domain 2 4 19 22 [Reporter]SCO1329 (1J17)_PCR/6968 [Gene]SCO1329/6350 NC_003888 2SCG61.11c, unknown, len: 77aa; 2 3 3 3 [Reporter]SCP1.128 (19N6)_PCR/4582 [Gene]SCP1.128/4177 NC_003888 SCP1.128, unknown, len: 265aa; Contains a TTA encoded leucine at residue 43, possible target for bldA regulation. 2 3 3 2 [Reporter]SCP1.09c (20F6)_PCR/4581 [Gene]SCP1.09c/4176 NC_003888 SCP1.09c, unknown, len: 221aa; 2 3 2 22 [Reporter]SCO5190 (1F2)_PCR/4579 [Gene]SCO5190/4175 NC_003888 2SC3B6.14, possible DNA-binding protein, len: 122 aa; similar to TR:Q9X952 (EMBL:AJ239087) Streptomyces coelicolor hypothetical 9.7 kD protein WblE, 85 aa; fasta scores: opt: 183 z-score: 243.0 E(): 4.6e-06; 40.5% identity in 74 aa overlap and to TR:Q9S426 (EMBL:AF164439) Mycobacterium smegmatis WhmD, 129 aa; fasta scores: opt: 161 z-score: 213.2 E(): 0.00021; 29.0% identity in 93 aa overlap. Contains Pfam match to entry PF02178 AT_hook, DNA binding domain with preference for A/T rich regions and match to Prosite entry PS00354 HMG-I and HMG-Y DNA-binding domain (A+T-hook) 2 3 2 21 [Reporter]SCO0729 (2B2)_PCR/4578 [Gene]SCO0729/4174 NC_003888 3SC5B7.07, conserved hypothetical protein, len: 140 aa; similar to TR:O27449 (EMBL:AE000902) Methanobacterium thermoautotrophicum conserved protein MTH1398, 133 aa; fasta scores: opt: 122 z-score: 167.4 E(): 0.085; 26.7% identity in 116 aa overlap 2 3 2 20 [Reporter]SCO2612 (2N22)_PCR/4577 [Gene]SCO2612/4173 NC_003888 SCC88.23c, ndk, nucleoside diphosphate kinase, 137 aa; identical to previously sequenced SW:NDK_STRCO (EMBL:X95643) Streptomyces coelicolor nucleoside diphosphate kinase (EC 2.7.4.6) Ndk, 137 aa. Contains Pfam match to entry PF00334 NDK, Nucleoside diphosphate kinases and match to Prosite entry PS00469 Nucleoside diphosphate kinases active site 2 3 2 19 [Reporter]SCO1401 (3J22)_PCR/4576 [Gene]SCO1401/4172 NC_003888 SC1A8A.21c, cvnB4, unknown, len: 144 aa; similar to others from Streptomyces coelicolor and Mycobacterium tuberculosis e.g. TR:Q9X835 (EMBL:AL049727) hypothetical protein from Streptomyces coelicolor (139 aa) fasta scores; opt: 408, z-score: 500.5, E(): 1.9e-20, 47.6% identity in 143 aa overlap. Local genetic arrangement also seems to be conserved for the Streptomyces coelicolor paralogues 2 3 2 18 [Reporter]SCO6188 (4F22)_PCR/4575 [Gene]SCO6188/4171 NC_003888 SC2G5.09, possible transferase, len: 566aa; C-terminal region similar to SW:RFAC_SALTY lipopolysaccharide heptosyltransferase-1 (317aa) blast scores; Sum P(2)= 1.3e-09, Identities= 46 /150 (30%), Positives = 70 /150 (46%). 1 1 15 3 [Reporter]SCP1.02c (19M20)_PCR/310 [Gene]SCP1.02c/279 NC_003888 SCP1.02c, unknown, len: 401aa; 2 3 2 17 [Reporter]SCO7125 (5B22)_PCR/4574 [Gene]SCO7125/4170 NC_003888 SC4B10.26c, conserved hypothetical protein, len: 246 aa; highly similar to TR:Q9S1U9 (EMBL:AL109950) Streptomyces coelicolor hypothetical 25.2 kDa protein SCJ4.24c, 242 aa; fasta scores: opt: 643 z-score: 729.3 E(): 0; 45.1% identity in 244 aa overlap and to Streptomyces coelicolor SC4B10.09c, 248 aa; fasta scores: opt: 779 z-score: 733.5 E(): 0; 51.6% identity in 244 aa overlap 1 1 15 2 [Reporter]SCO5084 (20E20)_PCR/309 [Gene]SCO5084/278 NC_003888 SCBAC28G1.10, actII-3, putative membrane protein, len: 711 aa; identical to previously sequenced SW:MMLA_STRCO (EMBL:M64683) Streptomyces coelicolor putative membrane protein ActII-3, 711 aa. Contains possible hydrophobic membrane spanning regions 1 1 14 22 [Reporter]SCO1012 (1E16)_PCR/307 [Gene]SCO1012/277 NC_003888 2SCG2.25c, probable oxidoreductase, len: 266 aa; similar to SW:BDHA_ALCEU (EMBL:AF145230) Alcaligenes eutrophus D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) HbdH1, 258 aa; fasta scores: opt: 586 z-score: 655.1 E(): 5.8e-29; 39.3% identity in 257 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 1 1 14 21 [Reporter]SCO7332 (2A16)_PCR/306 [Gene]SCO7332/276 NC_003888 SC4G10.11c, glgBII, 1,4-alpha-glucan branching enzyme (EC 2.4.1.18), len: 741 aa. Previously sequenced and characterised: Streptomyces coelicolor TR:Q59832(EMBL:X73903). Highly similar to Streptomyces coelicolor TR:CAB72416(EMBL:AL138978) 1,4-alpha-glucan branching enzyme, GlgBI or SC6A11.16c (774 aa), fasta scores opt: 3752 z-score: 4230.6 E():0 73.5% identity in 727 aa overlap. Contains a Pfam match to entry PF00128 alpha-amylase, Alpha amylase. 1 1 14 20 [Reporter]SCO5927 (3M12)_PCR/305 [Gene]SCO5927/275 NC_003888 SC10A5.32c, unknown, len: 73 1 1 14 19 [Reporter]SCO7027 (4I12)_PCR/304 [Gene]SCO7027/274 NC_003888 SC1H10.16c, probable LacI-family transcriptional regulator, len: 339 aa. Highly similar to Streptomyces lividans TR:Q9ZI18 (EMBL:AF043654) LacI-GalR family regulatory protein, BxlR (338 aa), fasta scores opt: 2136 z-score: 2395.5 E(): 0 96.5% identity in 340 aa overlap and Escherichia coli SW:CYTR_ECOLI (EMBL:X03683) transcriptional repressor, CytR (341 aa), fasta scores opt: 553 z-score: 625.0 E(): 2.2e-27 32.7% identity in 330 aa overlap. Also highly similar to Streptomyces coelicolor TR:O86795(EMBL:AL031317) putative transcriptional regulator, SC6G4.32 (355 aa), fasta scores opt: 1318 z-score: 1239.5 E():0 60.2% identity in 329 aa overlap. Contains a Prosite hit to PS00356 Bacterial regulatory proteins, lacI family signature and Pfam matches to entries PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and entry PF00356 lacI, Bacterial regulatory proteins, lacI family. Also contains a putative helix-turn-helix motif situated between residues 1..22 (+6.62 SD) 1 1 14 17 [Reporter]SCO5804 (6A12)_PCR/302 [Gene]SCO5804/273 NC_003888 SC4H2.25, unknown, len: 182 aa; similar to hypothetical genes from a wide range of organisms e.g. YBAD_ECOLI P25538 hypothetical 17.2 kd protein in tsx-ribG (149 aa), fasta scores; opt: 426 z-score: 367.0 E(): 3.2e-13, 43.0% identity in 149 aa overlap 1 1 14 16 [Reporter]SCO6770 (7M8)_PCR/301 [Gene]SCO6770/272 NC_003888 SC6A5.19, probable DNA-binding protein, len: 204 aa; similar to several of undefined function eg. TR:O86784 (EMBL:AL031317) hypothetical protein from Streptomyces coelicolor (201 aa) fasta scores; opt: 297, z-score: 356.2, E(): 1.5e-12, (34.9% identity in 212 aa overlap). Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix. 1 1 14 15 [Reporter]SCO2480 (8I8)_PCR/300 [Gene]SCO2480/271 NC_003888 SC7A8.19, possible secreted protein, len: 210 aa. Similar to Streptomyces coelicolor SCM10.24, 221 aa; fasta scores: opt: 672 z-score: 568.7 E(): 2.9e-26; 50.2% identity in 211 aa overlap. Contains possible N-terminal region signal peptide sequence 1 1 14 14 [Reporter]SCO7752 (9E8)_PCR/299 [Gene]SCO7752/270 NC_003888 SC8D11.43c, possible integral membrane protein, len: 184 aa; similar to TR:Q9X7R2 (EMBL:AL049863) Streptomyces coelicolor possible membrane protein SC5H1.04c, 167 aa; fasta scores: opt: 505 z-score: 631.2 E(): 1.3e-27; 48.7% identity in 150 aa overlap. Contains possible hydrophobic membrane spanning regions 2 4 19 21 [Reporter]SCO5547 (2F17)_PCR/6967 [Gene]SCO5547/6349 NC_003888 SC1C2.28, gltX, glutamyl-tRNA synthetase, len: 494 aa; highly similar to many e.g. SYE_ECOLI glutamyl-tRNA synthetase (EC 6.1.1.17) (471 aa), fasta scores; opt: 453 z-score: 894.0 E(): 0, 37.1% identity in 493 aa overlap. Contains Pfam match to entry PF00749 tRNA-synt_1c, tRNA synthetases class I (E and Q), score 470.50, E-value 1.3e-137 2 4 19 20 [Reporter]SCO7637 (3B17)_PCR/6966 [Gene]SCO7637/6348 NC_003888 SC10F4.10c, secreted endoglucanase, len: 486 aa; highly similar to SW:GUNA_STRLI (EMBL:M82807) Streptomyces lividans endoglucanase CelA precursor (EC 3.2.1.4) CelA, 459 aa; fasta scores: opt: 2977 z-score: 3136.1 E(): 0; 97.4% identity in 461 aa overlap. Contains Pfam matches to entries PF00553 CBD_2, Cellulose binding domain and PF00150 cellulase, Cellulase (glycosyl hydrolase family 5) and matches to Prosite entries PS00561 Cellulose-binding domain, bacterial type and PS00659 Glycosyl hydrolases family 5 signature. Also contains possible N-terminal region signal peptide sequence 2 4 19 19 [Reporter]SCO7203 (4N13)_PCR/6965 [Gene]SCO7203/6347 NC_003888 SC2H12.02, possible integral membrane protein, len: 258 aa. Contains multiple possible membrane spanning hydrophobic domains. 2 4 19 18 [Reporter]SCO6565 (5J13)_PCR/6964 [Gene]SCO6565/6346 NC_003888 SC4B5.15, probable transcriptional regulator, len: 225aa; similar to many hypothetical /putative transcriptional regulators e.g. TR:O50514 (EMBL:AL009204) probable transcriptional regulator from Streptomyces coelicolor (231 aa), fasta scores; opt: 322, z-score: 457.0, E(): 3.7e-18, (35.2% identity in 199 aa overlap) and TR:P77347 (EMBL:D90785) NTA operon transcriptional regulator from Escherichia coli (221 aa), fasta scores; opt: 233, z-score: 348.7, E(): 4e-12, (27.1% identity in 192 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family, score 56.90, E-value 5e-16. 2 4 19 17 [Reporter]SCO7753 (6F13)_PCR/6963 [Gene]SCO7753/6345 NC_003888 SC5E9.01c, unknown, len: 93 aa,SC8D11.44c, unknown (fragment), len: >76 aa 2 4 19 16 [Reporter]SCO5647 (7B13)_PCR/6962 [Gene]SCO5647/6344 NC_003888 SC6A9.20c, probable ABC transporter integral membrane protein, len: 553 aa; similar to many e.g. Y4FN_RHISN probable ABC transporter permease protein (569 aa), fasta scores; opt: 296 z-score: 555.5 E(): 1e-23, 25.4% identity in 563 aa overlap. Contains 2x Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, scores 40.70, E-value 3.2e-08 and 46.50, E-value 6.1e-10 2 4 19 15 [Reporter]SCO2493 (8N9)_PCR/6961 [Gene]SCO2493/6343 NC_003888 SC7A8.32c, probable membrane protein, len: 402 aa; similar to TR:Q9WX28 (EMBL:AL079345) Streptomyces coelicolor putative membrane protein SCE68.03, 441 aa; fasta scores: opt: 1057 z-score: 1265.9 E(): 0; 44.8% identity in 384 aa overlap and SW:MDMB_STRMY (EMBL:M93958) Streptomyces mycarofaciens acyltransferase (EC 2.3.1.-) MdmB, 387 aa; fasta scores: opt: 211 z-score: 255.4 E(): 7.8e-07; 25.6% identity in 406 aa overlap 2 4 19 14 [Reporter]SCO6990 (9J9)_PCR/6960 [Gene]SCO6990/6342 NC_003888 SC8F11.16c, possible membrane transport protein, len: 325 aa. Similar to several Eukaryotic and Prokaryotic membrane transporters including: Homo sapiens SW:NTCI_HUMAN(EMBL:U10417) ileal sodium/bile acid co-transporter (348 aa), fasta scores opt: 413 z-score: 449.9 E(): 1.3e-17 33.7% identity in 249 aa overlap and Aquifex aeolicus TR:O67889(EMBL:AE000774) Na(+) dependent transporter (297 aa), fasta scores opt: 763 z-score: 824.8 E():0 42.9% identity in 289 aa overlap. Contains a Pfam match to entry PF01758 SBF, Sodium Bile acid symporter family. Contains multiple possible membrane spanning hydrophobic domains. 2 3 2 16 [Reporter]SCO7292 (6N18)_PCR/4573 [Gene]SCO7292/4169 NC_003888 SC5F8.02c, possible threonine dehydratase, len: 319 aa. Similar to many including: Escherichia coli SW:THD2_ECOLI(EMBL:M21312) threonine dehydratase catabolic (EC 4.2.1.16) TdcB (329 aa), fasta scores opt: 609 z-score: 676.6 E(): 3.2e-30 39.4% identity in 312 aa overlap. Contains a Prosite hit to Serine/threonine dehydratases pyridoxal-phosphate attachment site and a Pfam match to entry PF00291 S_T_dehydratase, Pyridoxal-phosphate dependent enzyme. 2 4 19 13 [Reporter]SCO7364 (10F9)_PCR/6959 [Gene]SCO7364/6341 NC_003888 SC9H11.18c, possible TetR-family transcriptional regulator, len: 221 aa. Previously sequenced: Streptomyces coelicolor TR:BAA82698 (EMBL:AB017438) Orf1 (187 aa). Differs with the previously published sequence in the C-terminus, possibly due to a frameshift in the original sequence. Similar to several e.g. Streptomyces glaucescens SW:TCMR_STRGA (EMBL:M80674) tetracenomycin C transcriptional repressor, TcmR (226 aa), fasta scores opt: 338 z-score: 403.7 E(): 5e-15 37.3% identity in 217 aa overlap. Contains a Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family with the putative helix-turn-helix motif situated between residues 54..75 (+4.96 SD). 2 3 2 15 [Reporter]SCO2128 (7J18)_PCR/4572 [Gene]SCO2128/4168 NC_003888 SC6G10.01c, partial CDS, unknown, len: >40aa;,SC6E10.22c, hypothetical protein, len: 390 aa; unknown function, similar to the N-terminal half of anion-transporter ATPases e.g. TR:O50593 (EMBL:AB004659), arsA, Acidiphilium multivorum plasmid pKW301 arsenite activated ATPase of translocating ATPase (583 aa), fasta scores; opt: 190 z-score: 208.0 E(): 0.00029, 27.3% identity in 267 aa overlap. Previously partially sequenced as SW:YGL1_STRCO (EMBL:X65932) Streptomyces coelicolor hypothetical protein (341 aa), identical to this sequence 2 4 19 12 [Reporter]SCO2553 (11B9)_PCR/6958 [Gene]SCO2553/6340 NC_003888 SCC77.20c, possible oxidoreductase, len: 364 aa. This CDS has been partially sequenced and the C-terminal 142 aa, of its predicted protein product, are identical to Streptomyces coelicolor TR:Q02797(EMBL:X68792) hypothetical protein in pth270 promoter (fragment). Also highly similar to Mycobacterium tuberculosis TR:O33323(EMBL:AL008967) oxidoreductase (344 aa), fasta scores opt: 912 z-score: 971.6 E(): 0 45.5% identity in 336 aa overlap and Bacillus subtilis TR:O05413(EMBL:U93875) 2-nitropropane dioxygenase (347 aa), fasta scores opt: 702 z-score:749.8 E(): 0 37.8% identity in 339 aa overlap. 2 3 2 14 [Reporter]SCO6866 (8F18)_PCR/4571 [Gene]SCO6866/4167 NC_003888 SC7F9.18c, unknown, len: 396 aa. Weakly similar to Deinococcus radiodurans TR:AAF11162 (EMBL:AE002004) conserved hypothetical protein (470 aa), fasta scores opt: 145 z-score: 156.5 E():0.26 25.1% identity in 407 aa overlap. 2 3 2 13 [Reporter]SCO7142 (9B18)_PCR/4570 [Gene]SCO7142/4166 NC_003888 SC9A4.04, hypothetical protein, len: 133 aa; similar to SW:YODB_BACSU (EMBL:AF006665) Bacillus subtilis hypothetical 12.8 kDa protein in OdhA-CtpA intergenic region YodB, 112 aa; fasta scores: opt: 258 z-score: 328.5 E(): 7.9e-11; 44.0% identity in 91 aa overlap. Contains Pfam match to entry PF01638 DUF24, Protein of unknown function DUF24 2 3 2 12 [Reporter]SCO2330 (10N14)_PCR/4569 [Gene]SCO2330/4165 NC_003888 SCC53.21, possible isochorismatase, len: 196 aa; region similar to many eg. SW:P15048 (ENTB_ECOLI) isochorismatase (EC 3.3.2.1) from Escherichia coli (285 aa) fasta scores; opt: 123, z-score: 154.2, E(): 0.41, 28.9% identity in 114 aa overlap. Also similar to TR:P96654 (EMBL:AB001488) hypothetical protein from Bacillus subtilis (180 aa) fasta scores; opt: 358, z-score: 433.4, E(): 1.1e-16, 36.7% identity in 177 aa overlap. Contains Pfam match to entry PF00857 Isochorismatase, Isochorismatase family. 2 3 2 11 [Reporter]SCO4497 (11J14)_PCR/4568 [Gene]SCO4497/4164 NC_003888 SCD35.04, possible membrane protein, len: 297 aa. Contains possible hydrophobic membrane spanning regions 2 3 2 10 [Reporter]SCO4245 (12F14)_PCR/4567 [Gene]SCO4245/4163 NC_003888 SCD8A.18c, hypothetical protein, len: 150 aa; similar to TR:Q988W8 (EMBL:AP003009) MLR6561 protein from Rhizobium loti (Mesorhizobium loti) (136 aa) fasta scores; opt: 360, Z-score: 445.9, E(): 3.3e-17, 44.882% identity (47.107% ungapped) in 127 aa overlap. Also similar to TR:Q9ZXK9 (EMBL:AB008550) Pseudomonas aeruginosa phage phi CTX ORF17, 114 aa; fasta scores: opt: 172 z-score: 230.4 E(): 2.1e-05; 30.2% identity in 106 aa overlap 2 3 2 9 [Reporter]SCO3225 (13B14)_PCR/4566 [Gene]SCO3225/4162 NC_003888 SCE8.18, absA1, two component sensor kinase, len: 571aa; sequenced previously therefore identical to TR:Q53893 (EMBL:U51332) AbsA1, predicted sensor histidine kinase which acts as part of a two component signal transduction system in the global regulation of antibiotic synthesis in Streptomyces coelicolor (571 aa) fasta scores; opt: 3782, z-score: 3585.7, E(): 0, (100.0% identity in 571 aa overlap). 2 3 2 8 [Reporter]SCO0750 (14N10)_PCR/4565 [Gene]SCO0750/4161 NC_003888 SCF81.09c, unknown, len: 59 aa 1 1 14 13 [Reporter]SCO7351 (10A8)_PCR/298 [Gene]SCO7351/269 NC_003888 SC9H11.05, possible AraC-family transcriptional regulator, len: 289 aa. Similar to several including: Streptomyces coelicolor TR:O69819(EMBL:AL023496) transcriptional regulator, SC1A6.14 (256 aa), fasta scores opt: 397 z-score: 464.2 E(): 2.2e-18 33.5% identity in 254 aa overlap and Escherichia coli SW:YEAM_ECOLI(EMBL:AE000274) hypothetical transcriptional regulator (273 aa), fasta scores opt: 438 z-score: 510.8 E(): 5.4e-21 33.2% identity in 232 aa overlap. Contains a Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family with the putative helix-turn-helix motif situated between residues 194..215 (+4.88 SD). Contains a TTA /leucine codon which is a possible target for bldA regulation. 2 3 2 7 [Reporter]SCO5015 (15J10)_PCR/4564 [Gene]SCO5015/4160 NC_003888 SCK15.17, possible secreted protein, len: 203 aa; similar to many other membrane proteins, e.g. TR:Q9S6B8 (EMBL:AF115336) Pseudomonas fluorescens major outer membrane protein OprF, 343 aa; fasta scores: opt: 247 z-score: 285.4 E(): 2e-08; 34.3% identity in 181 aa overlap. Contains Pfam match to entry PF00691 OmpA, OmpA family and possible N-terminal region signal peptide sequence 1 1 14 12 [Reporter]SCO5280 (11M4)_PCR/297 [Gene]SCO5280/268 NC_003888 SCCB12.04, possible ATP-binding protein, len: 1148 aa; similar to TR:CAB77406 (EMBL:AL160431) Streptomyces coelicolor putative ATP-binding protein SCD82.03, 966 aa; fasta scores: opt: 798 z-score: 834.6 E(): 0; 31.7% identity in 977 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 1 14 11 [Reporter]SCO4132 (12I4)_PCR/296 [Gene]SCO4132/267 NC_003888 SCD72A.18, possible secreted transglycosylase, len: 340aa; similar to TR:CAB72389 (EMBL:AL138977) putative secreted protein from Streptomyces coelicolor (377 aa) fasta scores; opt: 310, z-score: 341.7, E(): 1.4e-11, 33.1% identity in 254 aa overlap. Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family and Pfam match to entry PF01464 SLT, Transglycosylase SLT domain. Also contains a possible non-cleavable N-terminal signal sequence. 1 1 14 10 [Reporter]SCO3200 (13E4)_PCR/295 [Gene]SCO3200/266 NC_003888 SCE22.17, probable deoR-family transcriptional regulator, len: 330 aa; similar to TR:CAB81864 (EMBL:AL161691) Streptomyces coelicolor putative deoR-family transcriptional regulator SCD40A.19c, 339 aa; fasta scores: opt: 682 z-score: 765.6 E(): 0; 43.3% identity in 298 aa overlap and to Streptomyces coelicolor SC8G12.03c, 343 aa; fasta scores: opt: 761 z-score: 720.8 E(): 1.3e-34; 45.1% identity in 326 aa overlap. Contains match to Prosite entry PS00894 Bacterial regulatory proteins, deoR family signature and possible helix-turn-helix motif at residues 22..43 (+5.48 SD) 1 1 14 9 [Reporter]SCO1033 (14A4)_PCR/294 [Gene]SCO1033/265 NC_003888 SCG20A.13c, possible membrane protein, len: 94 aa; low similarity to TR:Q9WYF1 (EMBL:AE001713) Thermotoga maritima hypothetical 10.3 kD protein TM0315, 83 aa; fasta scores: opt: 95 z-score: 135.8 E(): 4.4; 29.7% identity in 74 aa overlap. Contains possible hydrophobic membrane spanning region 1 1 14 8 [Reporter]SCO0850 (14M24)_PCR/293 [Gene]SCO0850/264 NC_003888 SCM2.03, possible membrane protein, len: 141 aa. Contains possible membrane spanning hydrophobic domains. 1 1 14 7 [Reporter]SCO5148 (15I24)_PCR/292 [Gene]SCO5148/263 NC_003888 SCP8.11, possible membrane protein, len: 329 aa. Contains possible hydrophobic membrane spanning region 1 1 14 6 [Reporter]SCO3499 (16E24)_PCR/291 [Gene]SCO3499/262 NC_003888 SCE65.35c, possible integral membrane protein, len: 585 aa. Contains possible hydrophobic membrane spanning regions 1 1 14 5 [Reporter]SCO0956 (17A24)_PCR/290 [Gene]SCO0956/261 NC_003888 SCM11.11, unknown, len: 151 aa 1 1 14 4 [Reporter]SCP1.166 (18M20)_PCR/289 [Gene]SCP1.166/260 NC_003888 SCP1.166, possible ATP/GTP-binding protein, len: 300aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 2 4 19 11 [Reporter]SCO3948 (12N5)_PCR/6957 [Gene]SCO3948/6339 NC_003888 SCD78.15, possible two component sensor, len: 560 aa; similar to hypothetical proteins from Mycobacterium tuberculosis (TR:O53473 (EMBL:AL021899) putative membrane protein fasta scores; opt: 1257, z-score:1182.6, E():0, (41.7% identity in 554 aa overlap) and TR:P95194 (EMBL:Z83867) hypothetical 62.2kD protein, fasta scores; opt: 1194, z-score: 1014.6, E(): 0, (41.0% identity in 558 aa overlap)) and two component sensor protein from Rhodococcus sp.(TR:G3319300 (EMBL:AF064700) fasta scores; opt: 968, z-score: 881.2, E(): 0, (36.7% identity in 559 aa overlap) 2 4 19 10 [Reporter]SCO3262 (13J5)_PCR/6956 [Gene]SCO3262/6338 NC_003888 SCE39.12, unknown, len: 249aa; limited similarity to others of unknown function some of which are associated with bacteriophage eg. SW:VE31_LAMBD protein EA31 from bacteriophage lambda (296 aa) fasta scores; opt: 133, z-score: 164.9, E(): 0.071, (35.3% identity in 85 aa overlap). Contains TTA codon, possible target for bldA regulation. 2 4 19 9 [Reporter]SCO5172 (14F5)_PCR/6955 [Gene]SCO5172/6337 NC_003888 SCP8.35, possible hydrolase, len: 358 aa; similar to SW:ACOC_ALCEU (EMBL:M66060) Alcaligenes eutrophus dihydrolipoamide acetyltransferase component of acetoin cleaving system (EC 2.3.1.12) AcoC, 373 aa; fasta scores: opt: 224 z-score: 254.0 E(): 1.1e-06; 29.7% identity in 273 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 2 4 19 8 [Reporter]SCO1167 (15B5)_PCR/6954 [Gene]SCO1167/6336 NC_003888 2SCG11.01, probable helicase (fragment), len: >697 aa; similar to C-terminal region of TR:CAB60181 (EMBL:AL132824) Streptomyces coelicolor putative helicase SCAH10.27, 977 aa; fasta scores: opt: 2570 z-score: 2915.8 E(): 0; 65.9% identity in 698 aa overlap. Contains Pfam match to entry PF00176 SNF2_N, SNF2 and others N-terminal domain and to PF00271 helicase_C, Helicases conserved C-terminal domain,SCG8A.21, partial CDS, possible helicase, len: >223aa; similar to TR:CAB60181 (EMBL:AL132824) putative helicase from Streptomyces coelicolor (977 aa) fasta scores; opt: 601, z-score: 651.6, E(): 7.5e-29, 51.1% identity in 231 aa overlap. Contains possible membrane-spanning hydrophobic regions. 2 4 19 7 [Reporter]SCO0675 (16N1)_PCR/6953 [Gene]SCO0675/6335 NC_003888 SCF91.35, unknown, len: 884 aa. Similar to several Streptomyces coelicolor proteins e.g. TR:O86807 (EMBL:AL031031) putative regulatory protein SC7C7.02C (916 aa), fasta scores opt: 351 z-score: 351.3 E(): 3.4e-12 26.9% identity in 815 aa overlap and TR:Q9ZBJ0 (EMBL:AL035161) conserved hypothetical protein SC9C7.20 (860 aa), fasta scores opt: 579 z-score: 580.5 E(): 5.8e-25 30.5% identity in 731 aa overlap. Also similar in the C-terminus to several other regulatory proteins e.g. Synechocystis sp. (strain PCC 6803) TR:P73256 (EMBL:D90905) sigma factor SibG regulation protein RsbU (452 aa), fasta scores opt: 240 z-score: 244.4 E(): 3.1e-06 26.7% identity in 363 aa overlap. Contains a Pfam match to entry PF01590 GAF, GAF domain. 2 4 19 6 [Reporter]SCO3786 (17J1)_PCR/6952 [Gene]SCO3786/6334 NC_003888 SCH63.33, possible integral membrane protein, len: 172 aa; low similarity to TR:O86915 (EMBL:Y15759) Streptomyces rochei ORFA protein (fragment), 128 aa; fasta scores: opt: 221 z-score: 269.8 E(): 1.6e-07; 33.0% identity in 109 aa overlap. Contains possible hydrophobic membrane spanning regions 2 4 19 5 [Reporter]SCO0325 (18F1)_PCR/6951 [Gene]SCO0325/6333 NC_003888 SCF12.04, unknown, len: 121aa; 2 4 19 4 [Reporter]SCO6278 (19B1)_PCR/6950 [Gene]SCO6278/6332 NC_003888 SC1G7.04, possible integral membrane transport protein, len: 543 aa; similar to TR:Q9RMF9 (EMBL:AF201913) Streptomyces globisporus transmembrane membrane efflux protein SgcB, 521 aa; fasta scores: opt: 1451 Z-score: 1413.5 bits: 271.2 E(): 3.8e-71; 46.243% identity in 519 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 2 6 [Reporter]SCO0415 (16F10)_PCR/4563 [Gene]SCO0415/4159 NC_003888 SCF51.14, possible integral membrane protein, len: 120 aa. Contains possible hydrophobic membrane spanning regions 2 4 19 3 [Reporter]SCO1984 (19N21)_PCR/6949 [Gene]SCO1984/6331 NC_003888 SC3C9.19c, probable hydrolase, len: 465 aa; similar to SW:ATZA_PSESD (EMBL:U55933) Pseudomonas sp. atrazine chlorohydrolase (EC 3.8.1.8) AtzA, 474 aa; fasta scores: opt: 448 Z-score: 490.2 bits: 100.0 E(): 1.1e-19; 33.258% identity in 445 overlap. Contains Pfam match to entry PF01685 ATZ_TRZ, Chlorohydrolase 2 3 2 5 [Reporter]SCO1592 (17B10)_PCR/4562 [Gene]SCO1592/4158 NC_003888 SCI35.14c, hypothetical protein, len: 180 aa; similar to other hypothetical proteins containing a mutT domain e.g. YQKG_BACSU (185 aa), fasta scores; opt: 192 z-score: 280.5 E(): 2.2e-08, 29.2% identity in 168 aa overlap. Contains PS00893 mutT domain signature, Pfam match to entry PF00293 mutT, Bacterial mutT protein, score 28.70, E-value 1e-06, and TTA Leu codon: possible target for action of bldA 2 4 19 2 [Reporter]SCO2819 (20F21)_PCR/6948 [Gene]SCO2819/6330 NC_003888 SCBAC17F8.10c, hypothetical protein, len: 204 aa: similar to many eg. TR:Q9X8N0 (EMBL:AL049628) hypothetical protein SCE94.25 from Streptomyces coelicolor (233 aa) fasta scores; opt: 250, Z-score: 267.4, 36.709% identity (39.726% ungapped) in 158 aa overlap. 2 3 2 4 [Reporter]SCO0356 (18N6)_PCR/4561 [Gene]SCO0356/4157 NC_003888 SCF41.15, probable oxidoreductase, len: 263 aa. Shares a high level of sequence similarity with Comamonas testosteroni TR:P94681 (EMBL:U32622) toluenesulfonate zinc-independent alcohol dehydrogenase (252 aa), fasta scores opt: 506 z-score: 549.4 E(): 3e-23 39.8% identity in 244 aa overlap and Streptomyces coelicolor TR:O88068 (EMBL:AL031541) putative dehydrogenase SCI35.33C (260 aa), fasta scores opt: 500 z-score: 542.8 E(): 7e-23 36.4% identity in 253 aa overlap. Contains a Pfam match to entry PF00106 adh_short, short chain dehydrogenase, Pfam match to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and a PS00061 Short-chain dehydrogenases/reductases family signature 2 3 2 3 [Reporter]SCP1.114 (19J6)_PCR/4560 [Gene]SCP1.114/4156 NC_003888 SCP1.114, unknown, len: 152aa; 2 3 2 2 [Reporter]SCP1.219 (20B6)_PCR/4559 [Gene]SCP1.219/4155 NC_003888 SCP1.219, unknown, len: 173aa; similar to others from Streptomyces coelicolor eg. TR:Q9Z552 (EMBL:AL035212) hypothetical protein (162 aa) blastp scores; Identities = 60/162 (37%), Positives = 80/162 (49%) and SW:P14706 (YM2_STRCO) mini-circle hypothetical 13.3 kDa protein (122 aa) blastp scores; Identities = 38/118 (32%), Positives = 60/118 (50%). 2 3 1 22 [Reporter]SCO5186 (1B2)_PCR/4557 [Gene]SCO5186/4154 NC_003888 2SC3B6.10, conserved hypothetical protein, len: 318 aa; similar to TR:O53345 (EMBL:AL021646) Mycobacterium tuberculosis hypothetical 33.8 kD protein MTV014.43c, 313 aa; fasta scores: opt: 334 z-score: 389.2 E(): 3.3e-14; 33.3% identity in 318 aa overlap 2 3 1 21 [Reporter]SCO4937 (1N22)_PCR/4556 [Gene]SCO4937/4153 NC_003888 SCK13.29c, hypothetical protein, len: 172 aa; similar to TR:CAC04235 (EMBL:AL391515) Streptomyces coelicolor conserved hypothetical protein SC4B10.30c, 156 aa; fasta scores: opt: 215 z-score: 274.7 E(): 9.2e-08; 30.9% identity in 139 aa overlap 2 3 1 20 [Reporter]SCO4950 (2J22)_PCR/4555 [Gene]SCO4950/4152 NC_003888 2SCK31.10, narI3, nitrate reductase gamma chain, len: 237 aa; highly similar to SW:NARI_BACSU (EMBL:Z49884) Bacillus subtilis nitrate reductase gamma chain (EC 1.7.99.4) NarI, 223 aa; fasta scores: opt: 792 z-score: 991.9 E(): 0; 49.6% identity in 234 aa overlap and to TR:Q9RI29 (EMBL:109989) Streptomyces coelicolor putative nitrate reductase delta chain NarI2 SCJ12.31, 256 aa; fasta scores: opt: 813 z-score: 891.7 E(): 0; 54.3% identity in 230 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 1 19 [Reporter]SCO7394 (3F22)_PCR/4554 [Gene]SCO7394/4151 NC_003888 SC10G8.22, possible integral membrane protein, len: 312 aa. Similar to several proteins of undefined function e.g. Deinococcus radiodurans TR:AAF11939(EMBL:AE002070) Conserved hypothetical protein (304 aa), fasta scores opt: 227 z-score: 259.6 E(): 4.8e-07 29.4% identity in 310 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence. 1 1 14 3 [Reporter]SCP1.207c (19I20)_PCR/288 [Gene]SCP1.207c/259 NC_003888 SCP1.207c, unknown, len: 900aa; 2 3 1 18 [Reporter]SCO6184 (4B22)_PCR/4553 [Gene]SCO6184/4150 NC_003888 SC2G5.05, possible transferase, len: 312aa, C-terminal region similar to C-terminal from many eg. TR:Q47594 (EMBL:D43637) mannosyl transferase B from Escherichia coli fasta scores; opt: 149, z-score: 178.9, E(): 0.011, (32.0% identity in 150 aa overlap). 1 1 14 2 [Reporter]SCO7700 (20A20)_PCR/287 [Gene]SCO7700/258 NC_003888 SCBAC12C8.01, possible cyclase, len: >137aa: strongly similar to C-terminus of TR:Q9F1V8 (EMBL:AB040071) cyclase from Streptomyces griseus (437 aa) fasta scores; opt: 788, Z-score: 962.7, 82.482% identity (82.482% ungapped) in 137 aa overlap. Previously sequenced as TR:Q9F1Y6 (EMBL:AB035202) ORF2.,SC1A4.08, unknown (fragment), len: >367 aa. High content in alanine and proline amino acid residues 1 1 13 22 [Reporter]SCO1007 (1A16)_PCR/285 [Gene]SCO1007/257 NC_003888 2SCG2.20c, possible oxidoreductase, len: 380 aa; similar to TR:O54177 (EMBL:AL021411) Streptomyces coelicolor putative oxidoreductase SC7H1.18, 397 aa; fasta scores: opt: 842 z-score: 944.5 E(): 0; 41.6% identity in 368 aa overlap. Contains Pfam matches to entries PF01494 FAD_binding_3, FAD binding domain and PF01360 Monooxygenase, Monooxygenase 1 1 13 21 [Reporter]SCO1638 (2M12)_PCR/284 [Gene]SCO1638/256 NC_003888 SCI41.21c, fkbP, peptidyl-prolyl cis-trans isomerase, len: 123 aa. Highly similar to SW:FKBP_STRCH (EMBL:M98428) Streptomyces chrysomallus FK506-binding protein (peptidyl-prolyl cis-trans isomerase - PPIase, 124 aa); fasta scores: opt: 678 z-score: 808.5 E(): 0; 78.2% identity in 124 aa overlap. Contains Pfam match to entry PF00254 FKBP, FKBP-type peptidyl-prolyl cis-trans isomerases also PS00453 FKBP-type peptidyl-prolyl cis-trans isomerase signature 1 and PS00454 FKBP-type peptidyl-prolyl cis-trans isomerase signature 2. Similarities with C-terminal domain of Streptomyces coelicolor StI41.22c (335 aa); fasta scores: opt: 263 z-score: 285.2 E(): 1.4e-10; 40.2% identity in 117 aa overlap. C-terminal contains signal peptide 1 1 13 20 [Reporter]SCO5923 (3I12)_PCR/283 [Gene]SCO5923/255 NC_003888 SC10A5.28c, ABC-transporter ATP-binding protein, len : 361 aa; similar to many eg. Streptomyces reticuli TR:P964 83 (EMBL:Y08921) MSIK PROTEIN involved in in cellobiose and maltose transport(377 aa), opt: 809 z-score: 1069.1 E(): 0 , 40.4% identity in 381 aa overlap. Contains PS00017 ATP/GT P-binding site motif A (P-loop). PS00211 ABC transporters f amily signature and Pfam match to entry ABC_tran PF00005, A BC transporters, score 35.90 1 1 13 19 [Reporter]SCO6027 (4E12)_PCR/282 [Gene]SCO6027/254 NC_003888 SC1C3.15c, probable acetyl-coA acetyltransferase (thiolase), len: 405 aa; similar to many e.g. E. coli TR:E242886 (EMBL:X97452 acetyl-coA acetyltransferase paaJ (401 aa), fasta scores; opt: 718 z-score: 1313.7 E(): 0, 42.6% identity in 408 aa overlap. Contains Pfam match to entry thiolase PF00108, Thiolases, score 410.31 1 1 13 18 [Reporter]SCO5458 (5A12)_PCR/281 [Gene]SCO5458/253 NC_003888 SC3D11.15, possible lipoprotein, len: 400aa; similar to many eg. TR:Q9X811 (EMBL:AL049497) putative secreted protein from Streptomyces coelicolor (424 aa) fasta scores; opt: 755, z-score: 799.1, E(): 0, 33.0% identity in 409 aa overlap. Also similar to neighbouring CDS SC3D11.14 fasta scores; opt: 1348, z-score: 1211.8, E(): 0, 53.3% identity in 394 aa overlap. Contains possible N-terminal signal sequence and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site 1 1 13 17 [Reporter]SCO7450 (6M8)_PCR/280 [Gene]SCO7450/252 NC_003888 SC5C11.07, possible secreted protein, len: 201 aa. Similar to Streptomyces coelicolor TR:CAB63184 (EMBL:AL133469) putative secreted protein, SCM10.23 (221 aa), fasta scores opt: 512 z-score: 527.4 E(): 5.9e-22 41.4% identity in 198 aa overlap. Contains a possible N-terminal signal sequence. 1 1 13 16 [Reporter]SCO6766 (7I8)_PCR/279 [Gene]SCO6766/251 NC_003888 SC6A5.15, unknown, len: 340 aa; similar to many of undefined function e.g. TR:P95416 (EMBL:D84475) NirJ, from a locus for heme D1 biosynthesis in Pseudomonas aeruginosa (387 aa) fasta scores; opt: 222, z-score: 264.2, E(): 2.1e-07, (24.2% identity in 207 aa overlap). 1 1 13 15 [Reporter]SCO5326 (8E8)_PCR/278 [Gene]SCO5326/250 NC_003888 SC6G9.07, hypothetical protein, len: 54 aa; unknown function, possible CDS suggested by GC frameplot, positional base preference and amino acid composition. Similar to part of (EMBL:AL049573), SCE39.23, S.coelicolor hypothetical protein (88 aa), fasta scores; opt: 165 z-score: 222.7 E(): 4.4e-05, 46.7% identity in 60 aa overlap. C-terminus overlaps with hypothetical CDS on opposite strand 2 4 18 22 [Reporter]SCO1021 (1F17)_PCR/6946 [Gene]SCO1021/6329 NC_003888 SCG20A.01, unknown (fragment), len: >110 aa,2SCG2.35, unknown (fragment), len: >72 aa 2 4 18 21 [Reporter]SCO2210 (2B17)_PCR/6945 [Gene]SCO2210/6328 NC_003888 SC10B7.05, glnII, glutamine synthetase, len: 343 aa; identical to previously sequenced TR:Q9X958 (EMBL:Y13833) Streptomyces coelicolor glutamine synthetase (EC 6.3.1.2) (glutamate-ammonia ligase) GlnII, 343 aa and highly similar to SW:GLN2_STRVR (EMBL:X52842) Streptomyces viridochromogenes glutamine synthetase II (EC 6.3.1.2) (glutamate-ammonia ligase II) GlnII, 343 aa; fasta scores: opt: 2196 z-score: 2572.0 E(): 0; 91.0% identity in 343 aa overlap. Contains Pfam match to entry PF00120 gln-synt, Glutamine synthetase and two matches to Prosite entries PS00180 Glutamine synthetase signature 1 and PS00181 Glutamine synthetase putative ATP-binding region signature 2 4 18 20 [Reporter]SCO6801 (3N13)_PCR/6944 [Gene]SCO6801/6327 NC_003888 SC1A2.10, possible LysR-family transcriptional regulator, len: 300 aa. Similar to many other regulators e.g. Streptomyces coelicolor TR:CAB62680 (EMBL:AL133422) putative LysR-family transcriptional regulatory protein, SCM1.22C (301 aa), fasta scores opt: 673 z-score: 751.5 E():0 40.3% identity in 300 aa overlap. Contains 2x Prosite hits to PS00044 Bacterial regulatory proteins, lysR family signature and a Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family with the putative helix-turn-helix motif situated between residues 17..38 (+4.44 SD). 2 4 18 19 [Reporter]SCO6208 (4J13)_PCR/6943 [Gene]SCO6208/6326 NC_003888 SC2G5.29, possible DNA-binding protein, len: 122 aa, contains probable helix-turn-helix at 97-118aa Score 1428(+4.05 SD). 2 4 18 18 [Reporter]SCO6380 (5F13)_PCR/6942 [Gene]SCO6380/6325 NC_003888 SC4A2.16c, unknown, len: 118 aa 2 4 18 17 [Reporter]SCO6547 (6B13)_PCR/6941 [Gene]SCO6547/6324 NC_003888 SC5C7.32, questionable ORF, len: 54 aa 2 4 18 16 [Reporter]SCO6779 (7N9)_PCR/6940 [Gene]SCO6779/6323 NC_003888 SC6A5.28c, unknown, len: 66 aa; designated based upon %GC and frameplot. 2 4 18 15 [Reporter]SCO2489 (8J9)_PCR/6939 [Gene]SCO2489/6322 NC_003888 SC7A8.28, probable tetR-family transcriptional regulator, len: 194 aa; similar to TR:CAB53122 (EMBL:AL109962) Streptomyces coelicolor putative transcriptional regulatory protein SCJ1.04, aa; fasta scores: opt: 184 z-score: 231.2 E(): 1.7e-05; 30.9% identity in 162 aa overlap and to SW:YCFQ_ECOLI (EMBL:AE000211) Escherichia coli hypothetical transcriptional regulator in NDH-MFD intergenic region YcfQ, 210 aa; fasta scores: opt: 282 z-score: 348.3 E(): 5.2e-12; 32.3% identity in 192 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 28..49 (+3.04 SD) 2 3 1 17 [Reporter]SCO6708 (5N18)_PCR/4552 [Gene]SCO6708/4149 NC_003888 SC4C6.18, possible membrane protein, len: 112 aa; unknown function, probable CDS suggested by positional base preference, GC frame plot and amino acid composition. Contains hydrophobic, possible membrane-spanning regions 2 4 18 14 [Reporter]SCO5498 (9F9)_PCR/6938 [Gene]SCO5498/6321 NC_003888 SC8D9.10, probable Glu-tRNA-Gln amidotransferase subunit C, gatC, len: 98aa; similar to many eg. TR:O06492 (EMBL:AF008553) Glu-tRNAGln amidotransferase subunit C, gatC, from Bacillus subtilis (101 aa) fasta scores; opt: 232. z-score: 294.8, E(): 4.1e-09, (42.1% identity in 95 aa overlap). 2 3 1 16 [Reporter]SCO2166 (6J18)_PCR/4551 [Gene]SCO2166/4148 NC_003888 SC5F7.35c, probable two component sensor kinase, len: 416 aa; similar to many others from Streptomyces coelicolor egs. TR:CAB40930 (EMBL:AL049630) putative two component sensor kinase (401 aa) fasta scores; opt: 731, z-score: 799.1, E(): 0, (36.7% identity in 398 aa overlap) and TR:Q53893 (EMBL:U51332) two component sensor kinase for the regulation of antibiotic production (571 aa) fasta scores; opt: 429, z-score: 469.2, E(): 8.4e-19, (29.5% identity in 407 aa overlap). Contains possible membrane spanning hydrophobic regions. 2 4 18 13 [Reporter]SCO7360 (10B9)_PCR/6937 [Gene]SCO7360/6320 NC_003888 SC9H11.14c, unknown, len: 200 aa. Previously sequenced: Streptomyces coelicolor TR:BAA82702(EMBL:AB017438) Orf5 (200 aa). Also similar to many other proteins of undefined function e.g. Neisseria meningitidis TR:AAF40874(EMBL:AE002400) conserved hypothetical protein (213 aa), fasta scores opt: 821 z-score: 1024.7 E(): 0 56.8% identity in 199 aa overlap. Contains a Prosite hit to PS01147 SUA5/yciO/yrdC family signature and a Pfam match to entry PF01300 Sua5_yciO_yrdC, SUA5/yciO/yrdC family. Contains a putative helix-turn-helix motif situated between residues. 2 3 1 15 [Reporter]SCO2673 (7F18)_PCR/4550 [Gene]SCO2673/4147 NC_003888 SC6D10.16, possible membrane protein, len: 907 aa; similar to TR:O86634 (EMBL:AL031155) Streptomyces coelicolor hypothetical 26.5 kD protein (fragment) SC3A7.33c, 259 aa; fasta scores: opt: 166 z-score: 178.0 E(): 0.017; 33.7% identity in 249 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence. Contains also possible hydrophobic membrane spanning regions 2 3 1 14 [Reporter]SCO6862 (8B18)_PCR/4549 [Gene]SCO6862/4146 NC_003888 SC7F9.14c, unknown, len: 114 aa. 2 3 1 13 [Reporter]SCO5854 (9N14)_PCR/4548 [Gene]SCO5854/4145 NC_003888 SC9B10.21, possible thiosulfate sulfurtransferase, len: 283 aa; similar to many eukaryotic and prokaryotic rhodaneses eg. THTR_HUMAN P25325 thiosulfate sulfurtransferase (EC 2.8.1.1) (296 aa), fasta scores; opt: 403 z-score: 389.9 E(): 1.5e-14, 35.0% identity in 286 aa overlap 2 3 1 12 [Reporter]SCO2326 (10J14)_PCR/4547 [Gene]SCO2326/4144 NC_003888 SCC53.17, hypothetical protein, len: 154 aa; similar to the C-terminal region of TR:Q9FBP7 (EMBL:AL391754) Streptomyces coelicolor putative secreted protein SCK7.02, 238 aa; fasta scores: opt: 477 Z-score: 516.1 bits: 102.2 E(): 4.1e-21; 64.356% identity (65.000% ungapped) in 101 aa overlap 2 3 1 11 [Reporter]SCO4103 (11F14)_PCR/4546 [Gene]SCO4103/4143 NC_003888 SCD17.07c. probable peptidase, len : 423 aa. Similar to many e.g. Sulfolobus solfataricus SW:CBPX_SULSO (EMBL; Z48497) thermostable carboxypeptidase (EC 3.4.17.-) (393 aa), fasta scores opt: 623 z-score: 708.6 E(): 4.1e-32 34.0% identity in 400 aa overlap. Contains a Pfam match to entry PF01546 Peptidase_M20. 2 3 1 10 [Reporter]SCO4241 (12B14)_PCR/4545 [Gene]SCO4241/4142 NC_003888 SCD8A.14c, possible proteinase precursor, len: 545 aa; similar to TR:Q54398 (EMBL:L42758) Streptomyces lividans proteinase SlpD, 539 aa; fasta scores: opt: 1116 z-score: 1193.2 E(): 0; 40.1% identity in 541 aa overlap and to TR:Q54399 (EMBL:L42759) Streptomyces lividans proteinase SlpE, 513 aa; fasta scores: opt: 1004 z-score: 1074.0 E(): 0; 47.7% identity in 539 aa overlap, and to TR:Q54398 (EMBL:AL035636) Streptomyces coelicolor SCH5.02c, proteinase SlpD, 539 aa; fasta scores: opt: 1116 z-score: 1004.4 E(): 0; 40.1% identity in 541 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold and correcty situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 2 3 1 9 [Reporter]SCO3119 (13N10)_PCR/4544 [Gene]SCO3119/4141 NC_003888 SCE41.28c, possible sensor kinase, len: 439 aa; similar to TR:O86631 (EMBL:AL031155) Streptomyces coelicolor two-component sensor SC3A7.30, 429 aa; fasta scores: opt: 296 z-score: 316.7 E(): 3.9e-10; 32.9% identity in 395 aa overlap. Contains possible hydrophobic membrane spanning regions at N-terminal domain 1 1 13 14 [Reporter]SCO7748 (9A8)_PCR/277 [Gene]SCO7748/249 NC_003888 SC8D11.39, hypothetical protein, len: 567 aa; similar to TR:CAC14487 (EMBL:AL446003) Streptomyces coelicolor putative DNA-binding protein SC5E9.07, 531 aa; fasta scores: opt: 473 z-score: 523.3 E(): 1.3e-21; 30.3% identity in 433 aa overlap. Contains match to Prosite entry PS00237 G-protein coupled receptors signature and 4x degenerate repeat: HAVVP 2 3 1 8 [Reporter]SCO1905 (14J10)_PCR/4543 [Gene]SCO1905/4140 NC_003888 SCI7.23c, unknown, len: 533aa; 1 1 13 13 [Reporter]SCO2781 (10M4)_PCR/276 [Gene]SCO2781/248 NC_003888 SCC105.12, hypothetical protein, len: 281 aa; similar to TR:CAB56689 (EMBL:AL121596) Streptomyces coelicolor hypothetical 28.7 kD protein, 259 aa; fasta scores: opt: 261 z-score: 302.6 E(): 2e-09; 34.9% identity in 255 aa overlap 1 1 13 12 [Reporter]SCO2371 (11I4)_PCR/275 [Gene]SCO2371/247 NC_003888 SCC8A.29, aceE2, pyruvate dehydrogenase E1 component (EC 1.2.4.1), len: 918 aa; strongly similar to many eg. SW:P06958 (ODP1_ECOLI) AceE, pyruvate dehydrogenase E1 component from Escherichia coli (886 aa) fasta scores; opt: 2723, z-score: 3143.0, E(): 0, 49.2% identity in 893 aa overlap and SW:Q10504 (ODP1_MYCTU) pyruvate dehydrogenase E1 component from Mycobacterium tuberculosis (901 aa) fasta scores; opt: 4067, z-score: 4697.1, E(): 0, 65.8% identity in 903 aa overlap. 1 1 13 11 [Reporter]SCO4788 (12E4)_PCR/274 [Gene]SCO4788/246 NC_003888 SCD63.20, possible integral membrane protein, len: 431 aa. Contains possible hydrophobic membrane spanning regions 1 1 13 10 [Reporter]SCO3196 (13A4)_PCR/273 [Gene]SCO3196/245 NC_003888 SCE22.13c, probable fructose-specific permease, len: 699 aa; similar to SW:PTFB_ECOLI (EMBL:M23196) Escherichia coli PTS system, fructose-specific IIBC component (EIIBC-fru) FruA 563 aa; fasta scores: opt: 1006 z-score: 1038.9 E(): 0; 43.7% identity in 583 aa overlap. Contains match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and possible hydrophobic membrane spanning regions in C-terminal domain 1 1 13 9 [Reporter]SCO1740 (13M24)_PCR/272 [Gene]SCO1740/244 NC_003888 SCI11.29, possible membrane protein, len: 354 aa; unknown function, weakly similar to TR:O07424 (EMBL:Z97050) Mycobacterium tuberculosis possible membrane protein (452 aa), fasta scores; opt: 190 z-score: 202.3 E(): 0.00062, 25.9% identity in 409 aa overlap. Contains hydrophobic, possible membrane-spanning regions 1 1 13 8 [Reporter]SCO0710 (14I24)_PCR/271 [Gene]SCO0710/243 NC_003888 SCF42.20c, possible branched-chain amino acid transport ATP-binding protein, len: 263 aa. Similar to many including: Pseudomonas aeruginosa SW:BRAG_PSEAE(EMBL:D90223) high-affinity branched-chain amino acid transport ATP-binding protein, BraG (233 aa), fasta scores opt: 642 z-score: 685.0 E(): 9.1e-31 46.7% identity in 240 aa overlap and Streptomyces coelicolor TR:CAB52068(EMBL:AL109732) putative branched chain amino acid transport ATP-binding protein, SC7H2.26 (238 aa), fasta scores opt: 724 z-score: 770.7 E():0 48.5% identity in 241 aa overlap. Also similar to the adjoining CDS on this cosmid, SCF42.19c (263 aa), fasta scores opt: 364 z-score: 308.0 E(): 9.7e-12 35.8% identity in 271 aa overlap. Contains Prosite hits to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Also contains a Pfam match to entry PF00005 ABC_tran, ABC transporter. 1 1 13 7 [Reporter]SCO3851 (15E24)_PCR/270 [Gene]SCO3851/242 NC_003888 SCH69.21c, probable glutamine amidotransferase, len: 212 aa; highly similar to many e.g. SW:TRPG_AZOBR TrpG, glutamine amidotransferase (anthranilate synthase II) from Azospirillum brasilense (196 aa) fasta scores; opt: 685, z-score: 811.9, E(): 0, (53.5% identity in 185 aa overlap) and TR:CAB38584 (EMBL:AL035654) probable anthranilate synthase II from Streptomyces coelicolor (200 aa) fasta scores; opt: 750, z-score: 887.7, E(): 0, (61.6% identity in 190 aa overlap). Contains Pfam match to entry PF00117 GATase, Glutamine amidotransferase class-I. 1 1 13 6 [Reporter]SCO1550 (16A24)_PCR/269 [Gene]SCO1550/241 NC_003888 SCL11.06c, possible small membrane protein, len: 60 aa. Contains possible hydrophobic membrane spanning regions 1 1 13 5 [Reporter]SCO3406 (17M20)_PCR/268 [Gene]SCO3406/240 NC_003888 SCE9.13c, conserved hypothetical protein, len: 352 aa; similar to many e.g. SW:MESJ_ECOLI (EMBL:D49445), mesJ, Escherichia coli cell cycle protein (432 aa), fasta scores; opt: 327 z-score: 356.8 E(): 1.5e-12, 34.4% identity in 282 aa overlap. Highly similar to SW:YZC5_MYCTU (EMBL:Z95436) Mycobacterium tuberculosis hypothetical protein (323 aa) (47.9% identity in 305 aa overlap) 2 4 18 12 [Reporter]SCO4373 (11N5)_PCR/6936 [Gene]SCO4373/6319 NC_003888 SCD10.05c, possible mut-like protein, len: 184 aa; similar to SW:YZGD_BACSU (EMBL:U17168) Bacillus subtilis hypothetical 45.4 kD protein in thiaminase I 5' region YgzD, 413 aa; fasta scores: opt: 225 z-score: 279.9 E(): 4.1e-08; 35.1% identity in 148 aa overlap. Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein and match to Prosite entry PS00893 mutT domain signature 2 4 18 11 [Reporter]SCO3944 (12J5)_PCR/6935 [Gene]SCO3944/6318 NC_003888 SCD78.11, hisC2, probable histidinol-phophate aminotransferase len: 369 aa, similar to eg, HIS8_BACSU (EMBL:U83322) histidinol-phophate aminotransferase from Bacillus subtilis (360 aa), fasta scores opt: 633, z-score: 785.8, E(): 0, (34.5% identity in 357 aa overlap). Also similar to S. coelicolor hisC; HIS8_STRCO (369 aa), fasta scores; opt: 567 z-score: 584.5 E(): 2.8e-25, 36.5% identity in 367 aa overlap. Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Contains Pfam match to entry PF00222 aminotran_2, Aminotransferases class-II, score 125.50, E-value 9.5e-34 2 4 18 10 [Reporter]SCO3065 (13F5)_PCR/6934 [Gene]SCO3065/6317 NC_003888 SCE25.06, hypothetical protein, len: 145 aa; similar to TR:CAB86125 (EMBL:AL163003) Streptomyces coelicolor hypothetical 16.0 kD protein, SCC24.31c, 142 aa; fasta scores: opt: 338 z-score: 419.4 E(): 6.6e-16; 42.6% identity in 141 aa overlap 2 4 18 9 [Reporter]SCO0907 (14B5)_PCR/6933 [Gene]SCO0907/6316 NC_003888 SCM1.40c, possible dehydrogenase, len: 296 aa. Similar to many including: Pseudomonas cruciviae TR:Q9ZNH7 (EMBL:AB015439) D-threonine dehydrogenase (fragment) (292 aa), fasta scores opt: 458 z-score: 510.7 E(): 4.5e-21 33.0% identity in 297 aa overlap. 2 4 18 8 [Reporter]SCO1561 (15N1)_PCR/6932 [Gene]SCO1561/6315 NC_003888 SCL11.17, possible integral membrane protein, len: 119 aa. Contains possible hydrophobic membrane spanning regions 2 4 18 7 [Reporter]SCO1178 (16J1)_PCR/6931 [Gene]SCO1178/6314 NC_003888 SCG11A.09c, unknown, len: 149 aa 2 4 18 6 [Reporter]SCO1624 (17F1)_PCR/6930 [Gene]SCO1624/6313 NC_003888 SCI41.07c, probable acetyltransferase, len: 165 aa. Similar to various putative acetyltransferases, e.g. SW:RIML_ECOLI (EMBL:X15860) Escherichia coli ribosomal-protein-serine acetyltransferase, 179 aa; fasta scores: opt: 181 z-score: 247.2 E(): 2.1e-06; 28.0% identity in 132 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 2 4 18 5 [Reporter]SCO0323 (18B1)_PCR/6929 [Gene]SCO0323/6312 NC_003888 SCF12.02c, unknown, len: 200aa; 2 3 1 7 [Reporter]SCO0563 (15F10)_PCR/4542 [Gene]SCO0563/4139 NC_003888 SCF73.10c, unknown, len: 477 aa; similar to TR:Q9RSL6 (EMBL:AE002046) from Deinococcus radiodurans fasta scores; opt: 1724, Z-score: 1902.8, 53.669% identity (54.468% ungapped) in 477 aa overlap. 2 4 18 4 [Reporter]SCO1880 (18N21)_PCR/6928 [Gene]SCO1880/6311 NC_003888 SCI39.27c, probable secreted pectate lyase, len: 444 aa; similar to SW:PEL_BACS (EMBL:X74880) Bacillus subtilis pectate lyase precursor (EC 4.2.2.2) Pel, 420 aa; fasta scores: opt: 1127 Z-score: 1249.8 bits: 240.3 E(): 5e-62; 42.431% identity in 436 aa overlap. Contains 2x Pfam matches to entry PF00544 pec_lyase, Pectate lyase. Contains also possible N-terminal region signal peptide sequence 2 3 1 6 [Reporter]SCO3743 (16B10)_PCR/4541 [Gene]SCO3743/4138 NC_003888 SCH22A.21, hypothetical protein, len: 213 aa; similar to various hypothetical proteins, e.g. TR:P74231 (EMBL:D90913) Synechocystis sp. hypothetical 23.5 kD protein, 211 aa; fasta scores: opt: 217 z-score: 247.2 E(): 2.4e-06; 30.7% identity in 192 aa overlap 2 4 18 3 [Reporter]SCP1.301 (19J21)_PCR/6927 [Gene]SCP1.301/6310 NC_003888 SCP1.301, possible 4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.-), len: 338aa; part of the 3-hydroxyphenylpropionate degradation pathway, strongly similar to many eg. SW:P51020 (MHPE_ECOLI) 4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.-) from Escherichia coli (337 aa) fasta scores; opt: 865, z-score: 959.9, E(): 0, 41.8% identity in 328 aa overlap and TR:Q9X9Q0 (EMBL:Y18574) Nikb protein from Streptomyces tendae (357 aa) fasta scores; opt: 1441, z-score: 1593.4, E(): 0, 67.4% identity in 319 aa overlap. Contains Pfam match to entry PF00682 HMGL-like, HMGL-like. 2 3 1 5 [Reporter]SCO5142 (17N6)_PCR/4540 [Gene]SCO5142/4137 NC_003888 SCP8.05, possible secreted protein, len: 119 aa. Contains possible coiled-coil region at approx residues 77..95 and N-terminal region signal peptide sequence 2 3 1 4 [Reporter]SCO3647 (18J6)_PCR/4539 [Gene]SCO3647/4136 NC_003888 SCH10.25c, conserved hypothetical protein, len: 143 aa; unknown function, similar to TR:O06310 (EMBL:Z95324) Mycobacterium tuberculosis hypothetical protein (145 aa), fasta scores; opt: 497 z-score: 431.5 E(): 1e-16, 55.8% identity in 138 aa overlap and TR:O69598 (EMBL:AL023514) Mycobacterium leprae hypothetical protein (137 aa) (56.2% identity in 130 aa overlap) 2 3 1 3 [Reporter]SCP1.284 (19F6)_PCR/4538 [Gene]SCP1.284/4135 NC_003888 SCP1.284, unknown, len: 115aa; 1 3 23 22 [Reporter]SCO1759 (1I22)_PCR/4536 [Gene]SCO1759/4134 NC_003888 2SCI34.12c, possible transferase, len: 220 aa; similar to TR:O67841 (EMBL:AE000770) Aquifex aeolicus 2-acylglycerophosphoethanolamine acyltransferase Aas, 211 aa; fasta scores: opt: 325 z-score: 388.0 E(): 3.6e-14; 33.3% identity in 189 aa overlap. Contains Pfam match to entry PF01553 Acyltransferase, Acyltransferase 1 3 23 21 [Reporter]SCO2786 (2E22)_PCR/4535 [Gene]SCO2786/4133 NC_003888 SCC105.17c, hexA, beta-N-acetylhexosaminidase precursor, len: 535 aa; highly similar to TR:O85361 (EMBL:AF063001) Streptomyces plicatus B-N-acetylhexosaminidase, Hex, 561 aa; fasta scores: opt: 2991 z-score: 3318.1 E(): 0; 93.2% identity in 469 aa overlap and to TR:BAA8876 (EMBL:AB015350) Streptomyces thermoviolaceus beta-N-acetylglucosaminidase precursor NagB, 541 aa; fasta scores: opt: 2856 z-score: 3168.6 E(): 0; 76.7% identity in 537 aa overlap. Contains Pfam match to entry PF00728 Glyco_hydro_20, Glycosyl hydrolase family 20 and possible N-terminal region signal peptide sequence 1 3 23 20 [Reporter]SCO7387 (3A22)_PCR/4534 [Gene]SCO7387/4132 NC_003888 SC10G8.14, unknown, len: 168 aa; similar to another from Streptomyces coelicolor SC8D11.07 TR:Q9AK12 (EMBL:AL512944) hypothetical protein fasta scores; opt: 426, Z-score: 537.3, E(): 2.7e-22, 46.763% identity (46.763% ungapped) in 139 aa overlap. 1 3 23 19 [Reporter]SCO7611 (4M18)_PCR/4533 [Gene]SCO7611/4131 NC_003888 SC2H2.09, conserved hypothetical protein, len: 237 aa; similar to TR:O06738 (EMBL:Y09476) Bacillus subtilis YitC protein, 228 aa; fasta scores: opt: 320 z-score: 384.6 E(): 6.9e-14; 32.2% identity in 199 aa overlap 1 1 13 4 [Reporter]SCP1.21c (18I20)_PCR/267 [Gene]SCP1.21c/239 NC_003888 SCP1.21c, unknown, len: 285aa; 1 3 23 18 [Reporter]SCO6703 (5I18)_PCR/4532 [Gene]SCO6703/4130 NC_003888 SC4C6.13c, pcaI, probable 3-oxoadipate CoA-transferase subunit A, len: 260 aa; highly similar to many CoA transferases e.g. SW:PCAI_PSEPU (EMBL:M88763), pcaI, Pseudomonas putida 3-oxoadipate CoA-transferase subunit A (231 aa), fasta scores; opt: 337 z-score: 379.8 E(): 8e-14, 42.6% identity in 242 aa overlap. Highly similar to SW:SCOA_MYCTU (EMBL:Z95556), scoA, Mycobacterium tuberculosis probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (248 aa) (69.9% identity in 249 aa overlap). Contains Pfam match to entry PF01144 CoA_trans, Coenzyme A transferase and PS01273 CoA transferases signature 1 1 1 13 3 [Reporter]SCO4056 (19E20)_PCR/266 [Gene]SCO4056/238 NC_003888 2SCD60.22, putative secreted protein, len: 63 aa. Contains possible N-terminal region signal peptide sequence 1 1 13 2 [Reporter]SCO5093 (20M16)_PCR/265 [Gene]SCO5093/237 NC_003888 SCBAC28G1.19c, ORF7, putative mini-circle protein, len: 165 aa; identical to previously sequenced TR:Q9X9V2 (EMBL:Y18817) Streptomyces coelicolor A3(2) putative mini-circle protein ORF7, 165 aa 1 1 12 22 [Reporter]SCO1273 (1M12)_PCR/263 [Gene]SCO1273/236 NC_003888 2SCG18.20c, probable reductase, len: 372 aa; similar to C-terminal domain of SW:HETM_ANASP (EMBL:L22883) Anabaena sp. polyketide synthase HetM, 506 aa; fasta scores: opt: 333 z-score: 389.0 E(): 3.5e-14; 35.7% identity in 395 aa overlap and to Streptomyces coelicolor 2SCG18.32c, 373 aa; fasta scores: opt: 241 z-score: 242.2 E(): 6.9e-08; 27.0% identity in 330 aa overlap. Contains TTA leucine codon, possible target for bldA regulation 1 1 12 21 [Reporter]SCO7066 (2I12)_PCR/262 [Gene]SCO7066/235 NC_003888 SC4G1.32, fadH, 2,4-dienoyl-CoA reductase [NADPH], len: 671 aa; highly similar to SW:FADH_ECOLI (EMBL:U93405) Escherichia coli 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34) FadH, 671 aa; fasta scores: opt: 2871 z-score: 3039.3 E(): 0; 62.8% identity in 667 aa overlap. Contains Pfam match to entry PF00724 oxidored_FMN, NADH:flavin oxidoreductase / NADH oxidase family 1 1 12 20 [Reporter]SCO3876 (3E12)_PCR/261 [Gene]SCO3876/234 NC_003888 SCH18.13c, recF, DNA replication protein, len: 373 aa; identical to previously sequenced SW:RECF_STRC (EMBL:L27063) Streptomyces coelicolor DNA replication protein RecF, 373 aa. Contains 3x Pfam matches to entry PF00470 RecF, RecF protein and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop), PS00617 RecF protein signature 1 and PS00618 RecF protein signature 2 1 1 12 19 [Reporter]SCO6023 (4A12)_PCR/260 [Gene]SCO6023/233 NC_003888 SC1C3.11, unknown, len: 586 aa; some similarity to hypothetical proteins e.g. M. tuberculosis TR:O06628 (EMBL:Z95618) MTCY07H7A.03 (532 aa), fasta scores; opt: 885 z-score: 918.8 E(): 0, 36.0% identity in 500 aa overlap. Also similar to two genes linked to capsular polysaccharide biosynthesis genes in Neisseria meningitidis: TR:Q51151 (EMBL:L09188) (373 aa), fasta scores; opt: 561 z-score: 537.2 E(): 1.1e-22, 29.8% identity in 326 aa overlap and TR:G2979674 (EMBL:AF019760) SACB (545 aa), fasta scores; opt: 678 z-score: 482.7 E(): 1.2e-19, 32.5% identity in 323 aa overlap. Also similar to upstream genes SC1C3.09 (E(): 0, 47.9% identity in 511 aa overlap) and SC1C3.10 (E(): 1.3e-23, 29.3% identity in 584 aa overlap) 1 1 12 18 [Reporter]SCO2077 (5M8)_PCR/259 [Gene]SCO2077/232 NC_003888 SC4A10.10c, hypothetical protein, len: 398 aa; unknown function, probable Gln-, Met- and Pro-rich CDS suggested by GC frame plot, positional base preference and amino acid composition. Contains probable coiled-coils from 23 to 68 (46 residues) (Max score: 1.868, probability 1.00) and from 217 to 294 (78 residues) (Max score: 1.445, probability 0.86) 1 1 12 17 [Reporter]SCO7446 (6I8)_PCR/258 [Gene]SCO7446/231 NC_003888 SC5C11.03, possible regulator, len: 146 aa. Similar to Streptomyces fradiae TR:Q9Z9I5 (EMBL:AB024056) beta-lactamase regulator protein (139 aa), fasta scores opt: 215 z-score: 266.6 E(): 2e-07 36.6% identity in 131 aa overlap and Streptomyces coelicolor TR:Q9X7S3 (EMBL:AL049863) putative regulator protein, SC5H1.15C (151 aa), fasta scores opt: 347 z-score: 419.9 E(): 5.7e-16 44.4% identity in 144 aa overlap. 1 1 12 16 [Reporter]SCO7256 (7E8)_PCR/257 [Gene]SCO7256/230 NC_003888 SC5H1.36, probable protease (putative secreted protein), len: 543 aa; similar to many e.g. TR:Q54398 (EMBL:L42758), SlpD, Streptomyces lividans protease, (539 aa), fasta scores; opt: 697 z-score: 769.7 E(): 0,35.8% identity in 545 aa overlap. Similar to other S.coelicolor proteases e.g. SlpD, SCH5.02c (EMBL:AL03563) proteinase (539 aa) (35.8% identity in 545 aa overlap). Contains possible N-terminal signal sequence. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold, score 55.00, E-value 1.6e-12 2 4 18 2 [Reporter]SCO3055 (20B21)_PCR/6926 [Gene]SCO3055/6309 NC_003888 SCBAC19G2.10, conserved hypothetical protein, len: 119aa: similar to many in Streptomyces coelicolor e.g. TR:O85701 (EMBL:AF072709) 3SCF60.11c conserved hypothetical protein (131 aa) fasta scores; opt: 322, Z-score: 387.9, 42.017% identity in 119 aa overlap and downstream neighbouring CDS SCBAC19G2.11 2 4 17 22 [Reporter]SCO1017 (1B17)_PCR/6924 [Gene]SCO1017/6308 NC_003888 2SCG2.31c, possible lipoprotein, len: 180 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains at N-terminal region possible hydrophobic membrane spanning regions 2 4 17 21 [Reporter]SCO4615 (2N13)_PCR/6923 [Gene]SCO4615/6307 NC_003888 SCD39.15c, integrase, len: 455 aa; identical to previously sequenced TR:Q53966 (EMBL:X71358) Streptomyces coelicolor Int, plasmid SLP1 DNA for xis and int genes, 455 aa. Contains Pfam match to entry PF00589 Phage_integrase, Phage integrase family and TTA leucine codon, possible target for bldA regulation 2 4 17 20 [Reporter]SCO6795 (3J13)_PCR/6922 [Gene]SCO6795/6306 NC_003888 SC1A2.04, cvnB7, unknown, len: 124 aa. Highly similar to several proteins of unknown function from Streptomyces coelicolor including: TR:Q9X835(EMBL:AL049727) SC9B1.15C (139 aa), fasta scores opt: 345 z-score: 434.8 E(): 8.2e-17 45.0% identity in 140 aa overlap and TR:CAB61281(EMBL:AL132991) SCF55.11C (152 aa), fasta scores opt:309 z-score:390.4 E(): 2.4e-14 41.0% identity in 134 aa overlap. 2 4 17 19 [Reporter]SCO5477 (4F13)_PCR/6921 [Gene]SCO5477/6305 NC_003888 SC2A11.11, probable oligopeptide-binding lipoprotein, len: 600 aa; similar to e.g. OPPA_BACSU oligopeptide-binding protein oppA precursor (545 aa), fasta scores; opt: 401 z-score: 242.5 E(): 3e-06, 25.5% identity in 554 aa overlap Also similar to S. coelicolor TR:P72407 (EMBL:U68036) bldKB (602 aa), fasta scores; opt: 702 z-score: 583.5 E(): 3e-25, 29.3% identity in 614 aa overlap. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site, and Pfam match to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5, score 45.60, E-value 7.2e-11 2 4 17 18 [Reporter]SCO6375 (5B13)_PCR/6920 [Gene]SCO6375/6304 NC_003888 SC4A2.11c, probable secreted protein, len: 220 aa; similar to a set of Mycobacterial secreted antigens e.g. MP83_MYCTU cell surface lipoprotein Mpt83 precursor (239 aa), fasta scores; opt: 376 z-score: 505.8 E(): 6.4e-21, 35.6% identity in 222 aa overlap 2 4 17 17 [Reporter]SCO7459 (6N9)_PCR/6919 [Gene]SCO7459/6303 NC_003888 SC5C11.16c, possible ABC transporter protein, ATP-binding component, len: 279 aa. Highly similar to several transporters e.g. Escherichia coli SW:FEPC_ECOLI(EMBL:X57471) ferric enterobactin transport ATP-binding protein, FepC (271 aa), fasta scores opt: 408 z-score: 486.3 E(): 1.1e-19 35.2% identity in 244 aa overlap and Streptomyces coelicolor TR:Q9X9Z4(EMBL:AL079332) putative ABC transporter ATP-binding protein, SCI5.06c (265 aa), fasta scores opt: 666 z-score: 788.8 E():0 44.2% identity in 260 aa overlap. Contains Prosite hits to PS00017 ATP/GTP-binding site motif A (P-loop)and PS00211 ABC transporters family signature. Also contains a Pfam match to entry PF00005 ABC_tran, ABC transporter. 2 4 17 16 [Reporter]SCO6775 (7J9)_PCR/6918 [Gene]SCO6775/6302 NC_003888 SC6A5.24c, probable transcriptional regulator, len: 206 aa; similar to many e.g. TR:O86510 (EMBL:AL031124) putative transcriptional regulator from Streptomyces coelicolor (200 aa) fasta scores; opt: 328, z-score: 399.0, E(): 6.4e-15, (34.9% identity in 186 aa overlap) and SW:BETI_ECOLI regulatory protein BetI from the osmoregulatory choline-glycine betaine pathway of Escherichia coli (195 aa) fasta scores; opt: 158, z-score: 198.8, E(): 0.0009, (25.0% identity in 180 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and probable helix-turn-helix (+3.31 SD) 32-53 aa 1 3 23 17 [Reporter]SCO7555 (6E18)_PCR/4531 [Gene]SCO7555/4129 NC_003888 SC5F1.09, possible solute binding lipoprotein, len: 436 aa; similar to TR:Q9L151 (EMBL:AL158061) Streptomyces coelicolor probable solute binding lipoprotein SC6D11.04c, 451 aa; fasta scores: opt: 386 z-score: 436.1 E(): 9.3e-17; 27.5% identity in 459 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal signal peptide sequence 2 4 17 14 [Reporter]SCO5494 (9B9)_PCR/6916 [Gene]SCO5494/6301 NC_003888 SC8D9.06, ligA, probable DNA ligase, len: 735aa; similar to many eg. SW:DNLJ_ECOLI DNA ligase from Escherichia coli (671 aa) fasta scores; opt: 1318, z-score: 1448.0, E(): 0, (41.6% identity in 694 aa overlap). Contains PS01055 NAD-dependent DNA ligase signature 1. Also contains Pfam match to entry PF00533 BRCT, BRCA1 C Terminus (BRCT) domain, score 41.70, E-value 1.7e-08. 1 3 23 16 [Reporter]SCO2668 (7A18)_PCR/4530 [Gene]SCO2668/4128 NC_003888 SC6D10.11, hypothetical protein, len: 452 aa; similar to TR:Y103_SYNY3 (EMBL:D64004) Synechocystis sp. hypothetical 45.8 kD protein SLL0103, 420 aa; fasta scores: opt: 265 z-score: 295.0 E(): 5.1e-09; 26.6% identity in 354 aa overlap 2 4 17 13 [Reporter]SCO2529 (10N5)_PCR/6915 [Gene]SCO2529/6300 NC_003888 SCC117.02, possible metalloprotease, len: 356 aa. Highly similar to several including: Serratia marcescens SW:SMP_SERMA(EMBL:M59854) extracellular minor metalloprotease precursor (EC 3.4.24.-) (352 aa), fasta scores opt: 659 z-score: 721.0 E(): 9e-33 41.2% identity in 291 aa overlap and Erwinia carotovora subsp. carotovora SW:PRT1_ERWCA(EMBL:M36651) extracellular metalloprotease precursor (EC 3.4.24.-) (347 aa), fasta scores opt: 1151 z-score: 1254.0 E():0 49.6% identity in 349 aa overlap. Contains a Pfam match to entry PF01447 Peptidase_M4, Thermolysin family peptidase and a Prosite hit to S00142 Neutral zinc metallopeptidases, zinc-binding region signature. 1 3 23 15 [Reporter]SCO1994 (8M14)_PCR/4529 [Gene]SCO1994/4127 NC_003888 SC7H2.08, possible integral membrane protein, len: 118aa; contains possible membrane spanning hydrophobic region. 1 3 23 14 [Reporter]SCO5846 (9I14)_PCR/4528 [Gene]SCO5846/4126 NC_003888 SC9B10.13c, putative secreted protein, len: 689 aa. Contains possible N-terminal region signal peptide sequence 1 3 23 13 [Reporter]SCO1934 (10E14)_PCR/4527 [Gene]SCO1934/4125 NC_003888 SCC22.16c, possible cytochrome oxidase assembly factor, len: 340 aa; similar to many e.g. TR:O33143 (EMBL:Z99125) cytochrome C oxidase assembly factor from Mycobacterium leprae (289 aa) fasta scores; opt: 925, z-score: 1077.3, E(): 0, (49.3% identity in 286 aa overlap) and SW:CYOE_ECOLI cytochrome O ubiquinol oxidase operon protein, CyoE from Escherichia coli (296 aa) fasta scores; opt: 520, z-score: 609.0, E(): 1.4e-26, (32.7% identity in 284 aa overlap). Contains Pfam match to entry PF01040 COX10_ctaB_cyoE, Cytochrome c oxidase assembly factor, Prosite match to PS00943 Cytochrome c oxidase assembly factor COX10/ctaB/cyoE signature and possible membrane spanning hydrophobic regions. 1 3 23 12 [Reporter]SCO4098 (11A14)_PCR/4526 [Gene]SCO4098/4124 NC_003888 SCD17.02c, probable acetyltransferase, len: 216 aa. Highly similar to many acetyltransferases involved in antibiotic resistance e.g. Enterococcus faecium SW:SATA_ENTFC (EMBL; L12033) streptogramin A acetyltransferase (EC 2.3.1.-) (209 aa), fasta scores opt: 610 z-score: 726.5 E(): 0 46.0% identity in 189 aa overlap and Enterococcus faecium TR:AAD44719 (EMBL; AF139725) SatG protein (214 aa), fasta scores opt: 665 z-score: 790.9 E(): 0 53.7% identity in 188 aa overlap. It is also highly similar to Streptomyces coelicolor SC5G9.03c (217 aa), fasta scores opt: 868 z-score: 924.4 E():0 62.0% identity in 205 aa overlap. Contains a Pfam match to entry PF00132 hexapep, Bacterial transferase hexapeptide (four repeats). 1 3 23 11 [Reporter]SCO4151 (12M10)_PCR/4525 [Gene]SCO4151/4123 NC_003888 SCD84.18c, possible acetyltransferase, len: 309 aa; similar to TR:O53831 (EMBL:AL022004) Mycobacterium tuberculosis hypothetical 33.6 kD protein MTV043.11, 315 aa; fasta scores: opt: 662 z-score: 773.0 E(): 0; 45.1% identity in 304 aa overlap and C-terminal region to SW:RIMI_ECOLI (EMBL:X06117) Escherichia coli ribosomal-protein-alanine acetyltransferase (EC 2.3.1.128) RimI, 148 aa; fasta scores: opt: 159 z-score: 195.2 E(): 0.0021; 31.3% identity in 112 aa overlap. Contains 2x Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 3 23 10 [Reporter]SCO3114 (13I10)_PCR/4524 [Gene]SCO3114/4122 NC_003888 SCE41.23c, conserved hypothetical protein, len: 298 aa; similar to TR:O07481 (EMBL:Z97071) Streptomyces reticuli AbpS protein, 311 aa; fasta scores: opt: 482 z-score: 473.6 E(): 7.1e-19; 35.0% identity in 294 aa overlap. Contains various coiled-coil regions at approx. residues 42..60 and 207..251 1 3 23 9 [Reporter]SCO0607 (14E10)_PCR/4523 [Gene]SCO0607/4121 NC_003888 SCF55.31 possible lipoprotein, len: 112 aa. Contains match to Prosite to entry correctly situated PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide 1 1 12 15 [Reporter]SCO4768 (8A8)_PCR/256 [Gene]SCO4768/229 NC_003888 SC6G4.46, probable two-component regulator, len: 203 aa; similar to many members of the luxR family e.g. DEGU_BACSU transcriptional regulatory protein DegU (229 aa), fasta scores; opt: 229 z-score: 546.3 E(): 3.4e-23, 30.9% identi ty in 223 aa overlap. Contains PS00622 Bacterial regulatory proteins, luxR family signature and Pfam matchs to entry PF00072 response_reg, Response regulator receiver domain, score 108.40, E-value 1.4e-28 and to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 65.60, E-value 1e-15 1 3 23 8 [Reporter]SCO1792 (15A10)_PCR/4522 [Gene]SCO1792/4120 NC_003888 SCI51.32c, possible 3-methyladenine DNA glycosylase, len: 213 aa; similar to several eukaryotic 3-methyladenine DNA glycosylases, but shorter at the N-terminus e.g. SW:3MG_HUMAN (EMBL:M74905) Homo sapiens (Human) DNA-3-methyladenine glycosylase (298 aa), fasta scores; opt: 410 z-score: 485.1 E(): 1.1e-19, 38.6% identity in 207 aa overlap. Also similar putative 3-methyladenine DNA glycosylases from mycobacteria e.g. SW:3MGH_MYCTU (EMBL:Z98268) Mycobacterium tuberculosis possible 3-methyladenine DNA glycosylase (203 aa) (43.0% identity in 193 aa overlap) 1 1 12 14 [Reporter]SCO7818 (9M4)_PCR/255 [Gene]SCO7818/228 NC_003888 SC8E7.15, possible oxidoreductase, len: 254 aa. Similar to several e.g. Escherichia coli SW:ENTA_ECOLI(EMBL:M24148) 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EC 1.3.1.28) (248 aa), fasta scores opt: 253 z-score: 302.9 E(): 2.1e-09 32.0% identity in 197 aa overlap and Streptomyces coelicolor TR:Q9S1Q4(EMBL:AL109972) putative oxidoreductase, SCJ9A.16 (253 aa), fasta scores opt: 622 z-score: 730.3 E(): 0 45.5% identity in 244 aa overlap. Contains a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature and a Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 1 1 12 13 [Reporter]SCO2771 (10I4)_PCR/254 [Gene]SCO2771/227 NC_003888 SCC105.02, hypothetical protein, len: 262 aa; similar to TR:Q9WX05 (EMBL:AL079345) Streptomyces coelicolor hypothetical 28.5 kD protein SCE68.27c, 271 aa; fasta scores: opt: 315 z-score: 382.9 E(): 6.8e-14; 42.8% identity in 269 aa overlap 1 1 12 12 [Reporter]SCO2271 (11E4)_PCR/253 [Gene]SCO2271/226 NC_003888 SCC75A.17c, possible membrane protein, len: 539 aa. Contains a possible N-terminal signal sequence, a possible membrane spanning hydrophobic domain and is rich in the amino acid Ala. 1 1 12 11 [Reporter]SCO4783 (12A4)_PCR/252 [Gene]SCO4783/225 NC_003888 SCD63.15, unknown, len: 107 aa 1 1 12 10 [Reporter]SCO2846 (12M24)_PCR/251 [Gene]SCO2846/224 NC_003888 SCE20.20c, possible transcriptional regulatory protein, len: 406 aa. Similar in parts to several proteins of the ROK (NagC/XylR) family including: Bacillus subtilis SW:GLK_BACSU (EMBL:D84432) glucokinase (EC 2.7.1.2) (glucose kinase) (321 aa), fasta scores opt: 320 z-score: 310.6 E(): 6.4e-10 34.1% identity in 164 aa overlap and Thermotoga maritima TR:Q9X1I0 (EMBL:AE001798) glucokinase (317 aa), fasta scores opt: 385 z-score: 370.1 E(): 3.1e-13 33.6% identity in 360 aa overlap. Contains a Prosite hits to PS01125 ROK family signature and PS01228 Hypothetical cof family signature 1. Also contains a Pfam match to entry PF00480 ROK, ROK family and a putative helix-turn-helix motif situated between residues 45..66 (+5.25 SD). 1 1 12 9 [Reporter]SCO0698 (13I24)_PCR/250 [Gene]SCO0698/223 NC_003888 SCF42.08c, unknown, len: 121 aa. 1 1 12 8 [Reporter]SCO2870 (14E24)_PCR/249 [Gene]SCO2870/222 NC_003888 SCE6.07, unknown, len: 98 aa. 1 1 12 7 [Reporter]SCO3898 (15A24)_PCR/248 [Gene]SCO3898/221 NC_003888 SCH24.20c, probable membrane protein, len: 425 aa; unknown function, similar to many putative membrane proteins e.g. SW:YO37_MYCTU (EMBL:Z80233) Mycobacterium tuberculosis hypothetical protein (441 aa), fasta scores; opt: 725 z-score: 832.9 E(): 0, 36.5% identity in 408 aa overlap and to resistance determinants e.g. TR:O3113 (EMBL:AF030344), tetV, Mycobacterium smegmatis tetracycline-resistance determinant (419 aa) (24.5% identity in 416 aa overlap). Similar to TR:O69824 (EMBL:AL023496) S.coelicolor possible integral membrane protein (630 aa) (27.0% identity in 429 aa overlap) and TR:O69821 (EMBL:AL023496) S.coelicolor probable transmembrane protein (431 aa) (23.1% identity in 428 aa overlap). Contains several hydrophobic, probable membrane-spanning regions 1 1 12 6 [Reporter]SCO0470 (16M20)_PCR/247 [Gene]SCO0470/220 NC_003888 SCF76.10, possible hydrolase, len: 302 aa. Weakly similar to hydrolases e.g. the N-terminal, domain of Streptomyces coelicolor TR:O86608 (EMBL:AL031155) 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase (449 aa), fasta scores opt: 387 z-score: 428.9 E(): 1.6e-16 33.7% identity in 258 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 1 3 23 7 [Reporter]SCO0659 (16M6)_PCR/4521 [Gene]SCO0659/4119 NC_003888 SCF91.19, possible DeoR-family transcriptional regulator, len: 368 aa. Similar to many DNA-binding regulatory proteins including: Streptomyces reticuli TR:CAB46346(EMBL:AJ009798) regulator CebR (350 aa), fasta scores opt: 287 z-score: 337.9 E(): 1.9e-11 30.6% identity in 359 aa overlap and Streptomyces lividans SW:REGL_STRLI (EMBL:X98242) transcription regulatory protein RegL (345 aa), fasta scores opt: 276 z-score: 325.3 E(): 9.6e-11 28.9% identity in 353 aa overlap. Contains Pfam matches to entries PF00455 deoR, Bacterial regulatory proteins, deoR family and Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family. In addition to a Prosite hit to PS00894 Bacterial regulatory proteins, deoR family signature with the putative helix-turn-helix motif situated between residues 23..44 (+3.43 SD). Also contains a probable coiled-coil from 21 to 48 (28 residues). 1 3 23 4 [Reporter]SCO4875 (19A6)_PCR/4518 [Gene]SCO4875/4118 NC_003888 2SCK8.01, probable sugar transferase (fragment), len: >256 aa; similar to SW:GTRB_BPSFX (EMBL:AF056939) Bacteriophage SfX (Shigella flexneri bacteriophage X) bactoprenol glucosyl transferase (EC 2.4.1.*) GtrB, 305 aa; fasta scores: opt: 659 z-score: 787.3 E(): 0; 42.3% identity in 246 aa overlap and to middle part of TR:O86666 (EMBL:AL031182) Streptomyces coelicolor putative sugar transferase SC4A2.10c, 478 aa; fasta scores: opt: 1022 z-score: 1213.9 E(): 0; 61.5% identity in 247 aa overlap. Contains possible hydrophobic membrane spanning regions at C-terminal domain,SCK20.16, probable transferase (fragment), len: >92 aa; similar to N-terminal region of SW:GTRB_BPSF5 (EMBL:U82619) Shigella flexneri bacteriophage V bactoprenol glucosyl transferase (EC 2.4.1.-) GtrB, 307 aa; fasta scores: opt: 264 z-score: 364.3 E(): 9.3e-13; 47.3% identity in 91 aa overlap and to N-terminal region of TR:O86666 (EMBL:AL031182) Streptomyces coelicolor putative sugar transferase SC4A2.10c, 478 aa; fasta scores: opt: 341 z-score: 431.2 E(): 2.4e-18; 61.1% identity in 90 aa overlap 1 3 23 3 [Reporter]SCO7515 (20M2)_PCR/4517 [Gene]SCO7515/4117 NC_003888 SCBAC25F8.07, unknown, len: 189 aa 1 3 22 22 [Reporter]SCO1147 (1E22)_PCR/4515 [Gene]SCO1147/4116 NC_003888 2SCG38.40, probable ABC transporter transmembrane protein (fragment), len: >35 aa,SCG8A.01, partial CDS, probable ABC transporter transmembrane subunit, len: >623aa; similar to many eg. TR:O87312 (EMBL:AF027770) FxtA iron sequestration protein from Mycobacterium smegmatis (574 aa) fasta scores; opt: 794, z-score: 866.3, E(): 0, 35.2% identity in 514 aa overlap. Contains Pfam match to entry PF00664 ABC_membrane, ABC transporter transmembrane region, Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite match to PS00211 ABC transporters family signature. Also contains possible membrane-spanning hydrophobic regions. 1 3 22 21 [Reporter]SCO7343 (2A22)_PCR/4514 [Gene]SCO7343/4115 NC_003888 SC4G10.22c, hemC, porphobilinogen deaminase (EC 4.3.1.8). len: 313 aa. Highly similar to many e.g. Escherichia coli SW:HEM3_ECOLI(EMBL:X04242) porphobilinogen deaminase (EC 4.3.1.8) (313 aa), fasta scores opt: 710 z-score: 839.8 E(): 0 42.2% identity in 303 aa overlap. Contains a Pfam match to entry PF01379 Porphobil_deam, Porphobilinogen deaminase. 1 3 22 20 [Reporter]SCO6423 (3M18)_PCR/4513 [Gene]SCO6423/4114 NC_003888 SC1A6.12c, putative lipoate-protein ligase, len: 388 aa; some similarity inC-terM to LPLA_ECOLI P32099 Escherichia coli putative lipoate-protein ligase A (337 aa), fasta scores; opt: 162 z-score: 223.4E(): 2.5e-05, 27.8% identity in 252 aa overlap 1 3 22 19 [Reporter]SCO7607 (4I18)_PCR/4512 [Gene]SCO7607/4113 NC_003888 SC2H2.05c, possible hydrolase, len: 393 aa; similar to SW:DAC_STRSQ (EMBL:M26842) Streptomyces sp. D-alanyl-D-alanine carboxypeptidase precursor (EC 3.4.16.4), 372 aa; fasta scores: opt: 313 z-score: 344.0 E(): 1.3e-11; 33.8% identity in 364 aa overlap 1 3 22 18 [Reporter]SCO6826 (5E18)_PCR/4511 [Gene]SCO6826/4112 NC_003888 SC4A9.03c, unknown, len: 346 aa. Similar to a protein of undefined function encoded within the Streptomyces peucetius daunorubicin-doxorubicin polyketide synthase gene cluster: TR:Q54816 (EMBL:L35560) DpsC (353 aa), opt: 662 z-score: 779.9 E(): 0 36.0% identity in 344 aa overlap. Also similar to Streptomyces sp. TR:Q55225 (EMBL:L34880) OrfC, a beta-keto acyl synthase III homologue (352 aa), fasta scores opt: 599 z-score: 706.2 E(): 6.3e-32 33.4% identity in 341 aa overlap 1 3 22 17 [Reporter]SCO7551 (6A18)_PCR/4510 [Gene]SCO7551/4111 NC_003888 SC5F1.05c, putative secreted protein, len: 67 aa. Contains possible N-terminal region signal peptide sequence 1 1 12 5 [Reporter]SCO0514 (17I20)_PCR/246 [Gene]SCO0514/219 NC_003888 SCF6.10, unknown, len: 217 aa. 1 3 22 16 [Reporter]SCO1414 (7M14)_PCR/4509 [Gene]SCO1414/4110 NC_003888 SC6D7.25. possible ankyrin-like protein, len: 134 aa. Similar to many Prokaryotic and Eukaryotic proteins containing ankyrin repeat domains e.g. Pseudomonas aeruginosa TR:Q51402(EMBL:U59457) AnkB (ankyrin) (180 aa), fasta scores opt: 263 z-score: 329.0 E(): 6e-11 40.7% identity in 118 aa overlap and Xiphophorus helleri TR:AAD21313(EMBL:AF132500) P13CDKN2X (124 aa), fasta scores opt: 192 z-score: 246.7 E(): 2.3e-06 34.7% identity in 121 aa overlap. Also similar to Streptomyces coelicolor (EMBL:AL121855) hypothetical protein SCF62.06 (131 aa), fasta scores opt:387 z-score: 407.2 E(): 2.5e-17 53.8% identity in 119 aa overlap. Contains 2xPfam match to entry PF00023 ank, Ank repeat. 1 1 12 4 [Reporter]SCO1686 (18E20)_PCR/245 [Gene]SCO1686/218 NC_003888 SCI30A.07, possible NTP pyrophosphohydrolase, len: 181 aa; similar to many e.g. TR:Q53738 (EMBL:X92429) from the puromycin biosynthetic pathway of Streptomyces alboniger (152 aa) fasta scores; opt: 170, z-score: 226.2, E(): 2.9e-05, (35.7% identity in 129 aa overlap). Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein. 1 1 12 3 [Reporter]SCO7510 (19A20)_PCR/244 [Gene]SCO7510/217 NC_003888 SCBAC25F8.02c, cypH, peptidyl-prolyl cis-trans isomerase, len: 165 aa; highly similar to SW:CYPH_YEAST (EMBL:X17505) Saccharomyces cerevisiae peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) Cpr1 or Cyp1 or Cph1 or Scc1 or YDR155C, 161 aa; fasta scores: opt: 798 Z-score: 947.8 bits: 181.7 E(): 3.5e-45; 70.988% identity in 162 aa overlap. Contains Pfam match to entry PF00160 pro_isomerase, Cyclophilin type peptidyl-prolyl cis-trans isomerase and matches to Prosite entries PS00170 Cyclophilin-type peptidyl-prolyl cis-trans isomerase signature and 2x PS00017 ATP/GTP-binding site motif A (P-loop) 1 1 12 2 [Reporter]SCO5079 (20I16)_PCR/243 [Gene]SCO5079/216 NC_003888 SCBAC28G1.05, actVA4, conserved hypothetical protein, len: 294 aa; identical to previously sequenced TR:Q53906 (EMBL:X58833) Streptomyces coelicolor 6 ActVA region genes of the actinorhodin biosynthetic gene cluster, ActVA4, 294 aa 1 1 11 22 [Reporter]SCO1269 (1I12)_PCR/241 [Gene]SCO1269/215 NC_003888 2SCG18.16c, probable pyruvate dehydrogenase beta subunit, len: 337 aa; similar to TR:Q9ZQY1 (EMBL:AF069910) Zea mays pyruvate dehydrogenase E1 beta subunit isoform 3 (EC 1.2.4.1) Pdh2, 374 aa; fasta scores: opt: 494 z-score: 584.2 E(): 4.7e-25; 30.3% identity in 337 aa overlap 1 1 11 21 [Reporter]SCO2206 (2E12)_PCR/240 [Gene]SCO2206/214 NC_003888 SC3H12.14, arsC, arsenate reductase (partial CDS), len: >47 aa; identical to N-terminal region of previously sequenced TR:Q9X954 (EMBL:Y13833) Streptomyces coelicolor arsenate reductase (fragment) ArsC, 117 aa and to SW:YGL1_STRVR (EMBL:X52842) Streptomyces viridochromogenes hypothetical 13.4 kD protein in GlnII region (ORF1), 119 aa,SC10B7.01, arsC, arsenate reductase (fragment), len: >117 aa; identical to previously sequenced TR:Q9X954 (EMBL:Y13833) Streptomyces coelicolor aresenate reductase (fragment), 117 aa, highly similar to SW:YGL1_STRVR (EMBL:X52842) Streptomyces viridochromogenes hypothetical 13.4 kD protein in GlnII region (ORF1), 119 aa; fasta scores: opt: 695 z-score: 873.2 E(): 0; 86.3% identity in 117 aa overlap and similar to SW:ARSC_ECOLI (EMBL:U00039;) Escherichia coli arsenate reductase (arsenical pump modifier) ArsC, 141 aa; fasta scores: opt: 202 z-score: 264.7 E(): 2.3e-07; 32.2% identity in 115 aa overlap 1 1 11 20 [Reporter]SCO7120 (3A12)_PCR/239 [Gene]SCO7120/213 NC_003888 SC4B10.21, qcrB2, ubiquinol-cytochrome C reductase cytochrome B subunit, len: 539 aa; similar to SW:QCRB_STRCO (EMBL:AL049497) Streptomyces coelicolor ubiquinol-cytochrome C reductase cytochrome B subunit QcrB or SC6G10.21c, 545 aa; fasta scores: opt: 2311 z-score: 2220.7 E(): 0; 59.4% identity in 537 aa overlap and to SW:QCRB_STRLI (EMBL:AF107888) Streptomyces lividans ubiquinol-cytochrome C reductase cytochrome B subunit QcrB, 549 aa; fasta scores: opt: 2148 z-score: 2064.6 E(): 0; 56.3% identity in 542 aa overlap. Contains Pfam match to entry PF00033 cytochrome_b_N, Cytochrome b(N-terminal)/b6/petB. Also contains possible hydrophobic membrane spanning regions 1 1 11 18 [Reporter]SCO2073 (5I8)_PCR/237 [Gene]SCO2073/212 NC_003888 SC4A10.06c, possible ribosomal large subunit pseudouridine synthase, len: 314 aa; similar to many e.g. SW:RLUC_ECOLI (EMBL:AE000209), RluC, Escherichia coli ribosomal large subunit pseudouridine synthase C, fasta scores; opt: 415 z-score: 467.2 E(): 1.1e-18, 30.1% identity in 326 aa overlap and SW:RLUD_ECOLI (EMBL:U50134), RluD, Escherichia coli ribosomal large subunit pseudouridine synthase D (326 aa) (37.1% identity in 315 aa overlap). Highly similar to hypothetical proteins from mycobacteria e.g. MLCB458.15 (EMBL:AL049478), RluC, Mycobacterium leprae possible pseudouridine synthase (308 aa) (64.3% identity in 305 aa overlap). Contains Pfam matches to entry PF01479 S4, S4 domain and to entry PF00849 YABO, Hypothetical yabO/yceC/sfhB family 1 1 11 17 [Reporter]SCO2060 (6E8)_PCR/236 [Gene]SCO2060/211 NC_003888 SC4G6.29c, possible integral membrane transport protein, len: 306aa; weakly similar to many eg. TR:CAB42664 (EMBL:AL049819) putative integral membrane protein from Streptomyces coelicolor (266 aa) fasta scores; opt: 163, z-score: 184.2, E(): 0.0061, (25.3% identity in 273 aa overlap). Contains possible membrane spanning hydrophobic regions. Location directly downstream of a possible ABC transport ATP-binding protein sueggsts a transport function. 1 1 11 15 [Reporter]SCO7237 (8M4)_PCR/234 [Gene]SCO7237/210 NC_003888 SC7A12.04c, possible secreted protein, len: 119 aa. Contains a possible N-terminal signal sequence. 1 3 22 15 [Reporter]SCO1990 (8I14)_PCR/4508 [Gene]SCO1990/4109 NC_003888 SC7H2.04c, unknown, len: 237aa; similar to SW:Y08N_MYCTU hypothetical protein from Mycobacterium tuberculosis (350 aa) fasta scores; opt: 870, z-score: 990.4, E(): 0, (57.1% identity in 231 aa overlap). 1 3 22 14 [Reporter]SCO7527 (9E14)_PCR/4507 [Gene]SCO7527/4108 NC_003888 SC8G12.03c, possible DeoR-family transcriptional regulator, len: 343 aa. Similar to several e.g. Streptomyces coelicolor TR:CAB81864 (EMBL:AL161691) putative DeoR-family transcriptional regulator, SCD40A.19C (339 aa), fasta scores opt: 600 z-score: 674.6 E(): 4.1e-30 41.1% identity in 316 aa overlap. Contains a Prosite hit to PS00894 Bacterial regulatory proteins, deoR family signature with a putative helix-turn-helix motif situated between residues 31..52 (+2.89 SD). 1 3 22 13 [Reporter]SCO1930 (10A14)_PCR/4506 [Gene]SCO1930/4107 NC_003888 SCC22.12, possible integral membrane transport protein, len: 356aa; function predicted as it contains possible membrane spanning hydrophobic regions and is located alongside a possible ABC transport ATP-binding protein (SCC22.10). Similar to TR:O53148 (EMBL:AL021184) hypothetical protein from Mycobacterium tuberculosis (310 aa) fasta scores; opt: 527, z-score: 648.1, E(): 9.2e-29, (35.0% identity in 300 aa overlap). 1 3 22 12 [Reporter]SCO4332 (11M10)_PCR/4505 [Gene]SCO4332/4106 NC_003888 SCD12A.15c, possible integral membrane ATPase, len: 802 aa; similar to TR:Q9Z4W5 (EMBL:AL035654) Streptomyces coelicolor putative integral membrane ATPase SCE8.09, 796 aa; fasta scores: opt: 3320 z-score: 3591.6 E(): 0; 67.0% identity in 796 aa overlap and to TR:O93862 (EMBL:AF043332) Aspergillus nidulans plasma membrane H(+)ATPase PmaA, 990 aa; fasta scores: opt: 504 z-score: 543.3 E(): 8.4e-23; 25.4% identity in 808 aa overlap. Contains 2x Pfam match to entry PF00122 E1-E2_ATPase, E1-E2 ATPase and match to Prosite entry PS00154 E1-E2 ATPases phosphorylation site. Contains also possible hydrophobic membrane spanning regions 1 3 22 11 [Reporter]SCO4147 (12I10)_PCR/4504 [Gene]SCO4147/4105 NC_003888 SCD84.14, possible integral membrane protein, len: 287 aa. Contains possible hydrophobic membrane spanning regions 1 3 22 10 [Reporter]SCO3250 (13E10)_PCR/4503 [Gene]SCO3250/4104 NC_003888 SCE29.19c, probable integrase, len: 384aa; similar to many eg. SW:INTR_STRAM integrase from the integrated conjugative pSAM plasmid of Streptomyces ambofaciens (388 aa) fasta scores; opt: 445, z-score: 549.1, E(): 2.8e-23, (26.8% identity in 392 aa overlap). Contains Pfam match to entry PF00589 Phage_integrase, 'Phage' integrase family, score 88.00, E-value 1.9e-22. 1 3 22 9 [Reporter]SCO0747 (14A10)_PCR/4502 [Gene]SCO0747/4103 NC_003888 SCF81.06c, hypothetical protein, len: 166 aa; similar to various hypothetical proteins, e.g. TR:AAD36153 (EMBL:AE001768) Thermotoga maritima conserved hypothetical protein TM1076, 157 aa; fasta scores: opt: 293 z-score: 385.9 E(): 4e-14; 39.1% identity in 133 aa overlap and to TR:Q9CCZ0 (EMBL:AL583926) Mycobacterium leprae conserved hypothetical protein ML 2654, 165 aa; fasta scores: opt: 642 Z-score: 785.7 E(): 4e-36; 60.248% identity in 161 aa overlap 1 3 22 8 [Reporter]SCO3917 (15M6)_PCR/4501 [Gene]SCO3917/4102 NC_003888 SCH24.39c, hypothetical protein, len: 288 aa; unknown function, similar to TR:O06743 (EMBL:Y09476), yitH, Bacillus subtilis hypothetical protein (282 aa), fasta scores; opt: 288 z-score: 349.1 E(): 3.9e-12, 26.8% identity in 231 aa overlap and TR:P73419 (EMBL:D90906) Synechocystis sp. hypothetical protein (285 aa) (21.9% identity in 265 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family, score 17.40, E-value 0.02 1 3 22 7 [Reporter]SCO3208 (16I6)_PCR/4500 [Gene]SCO3208/4101 NC_003888 SCE8.01, possible secreted protein, len: 432 aa; similar to TR:CAC44281 (EMBL:AL596030) Streptomyces coelicolor hypothetical protein SCBAC17F8.09, 436 aa; fasta scores: opt: 555 Z-score: 578.7 E(): 1.3e-24; 33.991% identity in 456 aa overlap. Contains a possible N-terminal signal sequence 1 1 11 14 [Reporter]SCO7813 (9I4)_PCR/233 [Gene]SCO7813/209 NC_003888 SC8E7.10, unknown, len: 283 aa. Highly similar to multiple proteins of unknown function including: Mycobacterium tuberculosis TR:P96822 (EMBL:Z92770) hypothetical 34.3 kd protein (317 aa), fasta scores: opt: 607 z-score: 740.2 E():0 39.1% identity in 284 aa overlap and Streptomyces coelicolor TR:Q9ZBH0 (EMBL:AL035206) SC9B5.10 (303 aa), fasta scores: opt: 481 z-score: 587.9 E(): 2.9e-25 36.4% identity in 280 aa overlap 1 3 22 6 [Reporter]SCO3353 (17E6)_PCR/4499 [Gene]SCO3353/4100 NC_003888 SCE94.04c, unknown, len: 126aa; 1 1 11 13 [Reporter]SCO5126 (10E4)_PCR/232 [Gene]SCO5126/208 NC_003888 SC9E12.11, hypothetical protein, len: 295 aa; similar to TR:O50426 (EMBL:AL010186) Mycobacterium tuberculosis hypothetical 31.7 kDa protein MTV005.06, 303 aa; fasta scores: opt: 531 z-score: 607.8 E(): 2.2e-26; 44.4% identity in 306 aa overlap 1 1 11 12 [Reporter]SCO2267 (11A4)_PCR/231 [Gene]SCO2267/207 NC_003888 SCC75A.13, probable heme oxygenase, len: 215 aa. Highly similar to many Eukaryotic and several Prokaryotic heme oxygenases including: Gallus gallus (Chicken) SW:HO_CHICK (EMBL:X56201) (296 aa), fasta scores opt: 485 z-score: 601.9 E(): 3.8e-26 37.6% identity in 205 aa overlap and Corynebacterium diphtheriae SW:HMUO_CORDI (EMBL:U73860)(215 aa), fasta scores opt: 463 z-score: 577.0 E(): 9.2e-25 41.5% identity in 205 aa overlap. Contains a Pfam match to entry PF01126 Heme_oxygenase, Heme oxygenase. 1 1 11 11 [Reporter]SCO4441 (11M24)_PCR/230 [Gene]SCO4441/206 NC_003888 SCD6.19, possible DNA-binding protein, len: 295 aa; similar to many other S. coelicolor hypothetical proteins, e.g. TR:Q9X8K0 (EMBL:AL049841) Streptomyces coelicolor hypothetical 30.9 kD protein SCE9.28c, 274 aa; fasta scores: opt: 717 z-score: 834.5 E(): 0; 42.6% identity in 263 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and a possible helix-turn-helix motif at residues 37..58 (+5.84 SD) 1 1 11 10 [Reporter]SCO2841 (12I24)_PCR/229 [Gene]SCO2841/205 NC_003888 SCE20.15c, possible secreted protein, len: 246 aa; similar to TR:Q9L2D9 (EMBL:AL137187) Streptomyces coelicolor putative secreted protein SC7A8.16c, 210 aa; fasta scores: opt: 493 Z-score: 488.6 E(): 1.4e-19; 40.580% identity in 207 aa overlap. Contains a possible membrane spanning hydrophobic domain 1 1 11 9 [Reporter]SCO1632 (13E24)_PCR/228 [Gene]SCO1632/204 NC_003888 SCI41.15c, hypothetical integral membrane protein, len: 316 aa. Similarity to SW:Q10702 SW:YK93_MYCTU Mycobacterium tuberculosis unknown membrane protein RV2093c, 308 aa; fasta scores: opt: 638 z-score: 762.5 E():0; 40.1% identity in 289 aa overlap. Contains Pfam match to entry PF00902 UPF0032, MttB family. 1 1 11 8 [Reporter]SCO0806 (14A24)_PCR/227 [Gene]SCO0806/203 NC_003888 SCF43.17c, possible LacI-family transcriptional regulatory protein, len: 341 aa. Similar to many regulators including: Escherichia coli SW:RBSR_ECOLI (EMBL:M13169) ribose operon repressor, RbsR (329 aa), fasta scores opt: 646 z-score: 726.9 E(): 0 35.9% identity in 334 aa overlap and Streptomyces coelicolor TR:Q9Z509 (EMBL:AL035591) putative transcriptional regulator (LacI family) SCC54.16 (368 aa), fasta scores opt: 659 z-score: 740.7 E(): 0 39.2% identity in 337 aa overlap. Contains a Pfam matches to entries PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family and Pfam match to entry PF00356 lacI, Bacterial regulatory proteins, lacI family. Also contains a Prosite hit to PS00356 Bacterial regulatory proteins, lacI family signature. A putative helix-turn-helix motif situated between residues 5..26 (+6.47 SD) 1 1 11 7 [Reporter]SCO3253 (15M20)_PCR/226 [Gene]SCO3253/202 NC_003888 SCE39.03c, SpdD protein, len: 105aa; highly similar to TR:Q07196 (EMBL:AJ005260) SpdD protein from the integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens (104 aa) fasta scores; opt: 491, z-score: 476.3, E(): 3.2e-19, (72.1% identity in 104 aa overlap). Contains possible membrane spanning regions 1 1 11 6 [Reporter]SCO1896 (16I20)_PCR/225 [Gene]SCO1896/201 NC_003888 SCI7.14, unknown, len: 269aa; similar to many eg. SW:YTBQ_BACSU hypothetical protein from Bacillus subtilis (253 aa) fasta scores; opt: 262, z-score: 310.1, E(): 6.1e-10, (27.8% identity in 241 aa overlap). 1 1 11 5 [Reporter]SCO1746 (17E20)_PCR/224 [Gene]SCO1746/200 NC_003888 SCI11.35c, sal, secreted serine protease, len: 300 aa; highly similar to extracellular serine proteases from Streptomyces spp. Identical to TR:Q54392 (EMBL:L44109), Sal, Streptomyces lividans chymotrypsin-like serine protease (300 aa), fasta scores; opt: 1919 z-score: 1861.1 E(): 0, 99.7% identity in 300 aa overlap. Similar to SCI11.30c S.coelicolor probable secreted serine protease (360 aa) (52.2% identity in 278 aa overlap). Contains probable N-terminal signal sequence. Contains Pfam match to entry PF00089 trypsin, Trypsin, PS00134 Serine proteases, trypsin family, histidine active site and PS00135 Serine proteases, trypsin family, serine active site 1 4 20 12 [Reporter]SCO2558 (11E9)_PCR/6476 [Gene]SCO2558/5899 NC_003888 SCC77.25c, unknown, len: 224 aa. 1 4 20 11 [Reporter]SCO4473 (12A9)_PCR/6475 [Gene]SCO4473/5898 NC_003888 SCD65.16, possible cytochrome biogenesis related protein, len: 253 aa; similar to SW:CCDA_BACSU (EMBL:X87845) Bacillus subtilis cytochrome C-type biogenesis protein CcdA, 235 aa: fasta scores: opt: 454 z-score: 507.4 E(): 8.5e-21; 33.3% identity in 228 aa overlap. Contains match to Prosite entry PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Also contains possible hydrophobic membrane spanning regions 1 4 20 10 [Reporter]SCO3265 (13M5)_PCR/6474 [Gene]SCO3265/5897 NC_003888 SCE39.15c, unknown, len: 74aa; similar to TR:CAC44709 (EMBL:AL596251) conserved hypothetical protein SCBAC25E3.22 from Streptomyces coelicolor (74 aa) fasta scores; opt: 356, Z-score: 489.1, E(): 1.3e-19, 77.465% identity (77.465% ungapped) in 71 aa overlap. 1 4 20 9 [Reporter]SCO1829 (14I5)_PCR/6473 [Gene]SCO1829/5896 NC_003888 SCI8.14, possible membrane protein, len: 290 aa. Weakly similar to several proteins of undefined function e.g. Streptomyces coelicolor TR:CAB45478 (EMBL: AL079348) hypothetical 30.3 KD protein SC66T3.22 (278 aa), fasta scores opt: 394 z-score: 463.3 E(): 1.9e-18 33.8% identity in 272 aa overlap and Mycobacterium leprae TR:O69480 (EMBL: AL023635) hypothetical 30.0 KD protein (270 aa), fasta scores opt: 392 z-score: 461.2 E(): 2.5e-18 30.7% identity in 270 aa overlap. Note it is possible that codon 28 may be the true translational start site. Contains a possible membrane spanning hydrophobic domain 1 4 20 8 [Reporter]SCO0810 (15E5)_PCR/6472 [Gene]SCO0810/5895 NC_003888 SCF43.21c. possible ABC transporter permease, len: 346 aa. Similar to many permeases e.g. Bacillus subtilis SW:RBSC_BACSU(EMBL:Z25798) ribose transport system permease protein RbsC (323 aa), fasta scores opt: 591 z-score: 644.0 E(): 1.7e-28 33.4% identity in 302 aa overlap and Escherichia coli SW:RBSC_ECOLI(EMBL:L10328) ribose transport system permease protein RbsC (321 aa), fasta scores opt: 596 z-score: 649.4 E(): 8.6e-29 34.5% identity in 304 aa overlap. Also similar to the adjoining CDS, SCF43.20c, possible ABC transporter permease (346 aa), fasta scores opt: 545 z-score: 528.3 E(): 5e-24 33.4% identity in 335 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 1 4 20 7 [Reporter]SCO1736 (16A5)_PCR/6471 [Gene]SCO1736/5894 NC_003888 SCI11.25c, possible marR-family transcriptional regulator, len: 148 aa; weakly similar to hypothetical proteins e.g. SW:YW06_MYCTU (EMBL:Z74025) Mycobacterium tuberculosis hypothetical protein (143 aa), fasta scores; opt: 159 z-score: 204.1 E(): 0.00049, 31.9% identity in 135 aa overlap. An alternative start codon is present at codon 7. Contains probable helix-turn-helix motif at aa 53-74 (Score 1261, +3.48 SD). Contains Pfam match to entry PF01047 MarR, MarR family. Contains possible N-terminal region signal peptide sequence 1 4 20 6 [Reporter]SCO4942 (17M1)_PCR/6470 [Gene]SCO4942/5893 NC_003888 2SCK31.02c, possible regulatory protein, len: 226 aa; similar to TR:Q9KXX1 (EMBL:AL357432) Streptomyces coelicolor putative transcriptional regulator SCD95A.03c, 199 aa; fasta scores: opt: 414 z-score: 464.7 E(): 2.4e-18; 38.2% identity in 204 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 1 4 20 5 [Reporter]SCO3313 (18I1)_PCR/6469 [Gene]SCO3313/5892 NC_003888 SCE68.11c, conserved hypothetical protein, len: 305 aa; unknown function, N-terminal half similar to hypothetical proteins from Mycobacterium tuberculosis, Mycobacterium paratuberculosis and Streptomyces lividans, e.g. SW:Y08L_MYCTU (EMBL:Z77137) Mycobacterium tuberculosis hypothetical protein (149 aa), fasta scores; opt: 297 z-score: 354.1 E(): 2.1e-12, 42.7% identity in 124 aa overlap 1 4 20 4 [Reporter]SCP1.213c (19E1)_PCR/6468 [Gene]SCP1.213c/5891 NC_003888 SCP1.213c, possible secreted protein, len: 225aa; contains a possible cleavable N-terminal signal sequence. 1 4 20 3 [Reporter]SCO5349 (20A1)_PCR/6467 [Gene]SCO5349/5890 NC_003888 SCBAC5H2.18, probable integrase, len: 457 aa; similar to TR:CAC08268 (EMBL:AL392146) Streptomyces coelicolor integrase Int, 455 aa; fasta scores: opt: 1486 z-score: 1774.0 E(): 0; 50.3% identity in 455 aa overlap and to plasmid borne TR:Q53966 (EMBL:X71358) Streptomyces coelicolor plasmid SLP1 DNA for xis and int genes, Int, 455 aa; fasta scores: opt: 1486 z-score: 1774.0 E(): 0; 50.3% identity in 455 aa overlap 1 4 20 2 [Reporter]SCO3046 (20I21)_PCR/6466 [Gene]SCO3046/5889 NC_003888 SCBAC19G2.01, conserved hypothetical protein, len: >199aa: similar to neighbouring gene TR:Q9KZK1 (EMBL:AL353862) conserved hypothetical protein SCE34.25 fasta scores; opt: 558, Z-score: 599.1, 44.796% identity (50.254% ungapped) in 221 aa overlap. Contains possible hydrophobic membrane spanning regions,SCE34.27, conserved hypothetical protein (fragment), len: >357 aa; similar to TR:O68907 (EMBL:AF05830) Streptomyces roseofulvus FrnA, 770 aa; fasta scores: opt: 676 z-score: 755.5 E(): 0; 43.3% identity in 293 aa overlap, to Streptomyces coelicolor SCE34.25, 507 aa; fasta scores: opt: 1210 z-score: 1247.7 E(): 0; 51.5% identity in 359 aa overlap, to SCE34.24, 472 aa; fasta scores: opt: 659 z-score: 681.3 E(): 1.9e-32; 39.9% identity in 348 aa overlap, to SCE34.26, 507 aa; fasta scores: opt: 643 z-score: 664.4 E(): 1.7e-31; 39.5% identity in 354 aa overlap and to SCE34.23, 404 aa; fasta scores: opt: 554 z-score: 574.2 E(): 1.8e-26; 35.3% identity in 357 aa overlap 1 4 19 22 [Reporter]SCO1328 (1I17)_PCR/6464 [Gene]SCO1328/5888 NC_003888 2SCG61.10c, unknown, len: 285aa; 1 4 19 21 [Reporter]SCO0509 (2E17)_PCR/6463 [Gene]SCO0509/5887 NC_003888 SCF6.05, glpK2, glycerol kinase (ATP:glycerol 3-phosphotransferase)(EC 2.7.1.30), len: 507 aa. Highly similar to many glycerol kinases e.g. Mycobacterium tuberculosis TR: O69664 (EMBL; AL022121) (517 aa), fasta scores opt: 2606 z-score: 3028.8 E(): 0 75.3% identity in 503 aa overlap and Haemophilus influenzae SW:GLPK_HAEIN (EMBL; U32752) (502 aa), fasta scores opt: 1714 z-score: 1992.5 E():0 51.1% identity in 503 aa overlap. Contains Pfam match to entry PF00370 FGGY, FGGY family of carbohydrate kinases and Prosite hits to PS00933 FGGY family of carbohydrate kinases signature 1 and PS00445 FGGY family of carbohydrate kinases signature 2. Also contains a possible membrane spanning hydrophobic domain. 1 4 19 20 [Reporter]SCO7636 (3A17)_PCR/6462 [Gene]SCO7636/5886 NC_003888 SC10F4.09, possible membrane protein, len: 96 aa. Contains possible hydrophobic membrane spanning region 1 4 19 19 [Reporter]SCO6211 (4M13)_PCR/6461 [Gene]SCO6211/5885 NC_003888 SC9G1.01, possible uricase (partial CDS), len: >204 aa; similar to SW:URIC_ASPFL (EMBL:X61766) Aspergillus flavus uricase (EC 1.7.3.3) (urate oxidase), Uaz or Uox, 301 aa; fasta scores: opt: 49 z-score: 553.3 E(): 1.9e-23; 41.2% identity in 177 aa overlap. Contains Pfam match to entry PF01014 Uricase, Uricase,SC2G5.32, partial CDS, possible oxidoreductase, len: 141 aa; similar to the N-terminal region of many urate oxidases, a eukaryotic peroxisomal enzyme, e.g. SW:URIC_PAPHA urate oxidases from Papio hamadryas (Hamadryas baboon) (303 aa) fasta scores; opt: 282,z-score: 368.8, E(): 3e-13, (36.6% identity in 131 aaoverlap). 1 4 19 18 [Reporter]SCO6564 (5I13)_PCR/6460 [Gene]SCO6564/5884 NC_003888 SC4B5.14, fabH2, 3-oxoacyl-[acyl-carrier-protein] synthase II, len: 316 aa; similar to many e.g. TR:P72392 (EMBL:X86475) FabH, 3-oxoacyl-[acyl-carrier-protein] synthase III from Streptomyces coelicolor (343 aa), fasta scores; opt: 547, z-score: 1039.5, E(): 0, (41.9% identity in 334 aa overlap). 1 4 19 17 [Reporter]SCO6550 (6E13)_PCR/6459 [Gene]SCO6550/5883 NC_003888 SC5C7.35, probable oxidoreductase, len: 233 aa; similar to many e.g. TR:O54197 (EMBL:AJ000671) Streptomyces clavuligerus clavulanate-9-aldehyde reducatase (247 aa), fasta scores; opt: 340 z-score: 414.7 E(): 7.5e-16, 34.0% identity in 238 aa overlap. Contains Pfam match to entry PF00106 adh_short, Alcohol /other dehydrogenases, short chain type, score 129.10, E-value 8e-35 1 4 19 16 [Reporter]SCO5648 (7A13)_PCR/6458 [Gene]SCO5648/5882 NC_003888 SC6A9.19c, probable ABC transporter ATP binding protein, len: 348 aa; similar to many e.g. POTA_ECOLI spermidine /putrescine transport ATP-binding protein (378 aa), fasta scores; opt: 715 z-score: 832.8 E(): 0, 42.5% identity in 308 aa overlap. Contains PS00017 ATP /GTP-binding= transporters family signature and Pfam match to entry PF00005 ABC_tran, ABC transporters, score 226.00, E-value 5.5e-64 1 4 19 15 [Reporter]SCO2492 (8M9)_PCR/6457 [Gene]SCO2492/5881 NC_003888 SC7A8.31, possible membrane protein, len: 157 aa. Contains possible N-terminal region signal peptide sequence and C-terminal region possible hydrophobic membrane spanning region 1 4 19 14 [Reporter]SCO6989 (9I9)_PCR/6456 [Gene]SCO6989/5880 NC_003888 SC8F11.15c, unknown, len: 176 aa. 1 4 19 12 [Reporter]SCO2551 (11A9)_PCR/6454 [Gene]SCO2551/5879 NC_003888 SCC77.18, unknown, len: 114 aa. 1 4 19 11 [Reporter]SCO3947 (12M5)_PCR/6453 [Gene]SCO3947/5878 NC_003888 SCD78.14, cydCD, probable ABC transporter, len: 1172 aa; N-terminal similarity to CYDD_ECOLI (EMBL:L21749) ABC transporter ATP-binding protein from Escherichia coli (588 aa), fasta scores; opt: 736, z-score: 950.9, E(): 0, (39.2% identity in525 aa overlap) and CYDC_BACSU (EMBL :D83026) ABC transporter ATP-binding protein from Bacillus subtilis (567 aa), fasta scores; opt: 1174, z-score:949.4, E(): 0 (36.0% identity in 566 aa overlap). Also has full length similarity to MDR4_DROME (EMBL:M59076) multidrug resistance protein homolog from Drosophila melanogaster fasta scores; opt: 575,z-score: 856.3, E():0, (27.0% identity in 1162 aa overlap). Contains 2 x ATP /GTP-binding motifs (both PS00017), 2 x ABC transporter family signatures (both PS00211). Also contains Pfam match to entry PF00664 ABC transporter transmembrane region and 2 x Pfam matches to entry PF00005 (see misc_features). Contains possible hydrophobic membrane spanning regions 1 4 19 10 [Reporter]SCO3261 (13I5)_PCR/6452 [Gene]SCO3261/5877 NC_003888 SCE39.11, possible ATP-binding protein, len: 431 aa; limited similarity to others of unknown function eg. TR:Q47527 (EMBL:Z12832) hypothetical ATP-binding protein from Escherichia coli (492 aa) fasta scores; opt: 239, z-score: 271.8, E(): 7.9e-08, (32.0% identity in 153 aa overlap). Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 4 19 9 [Reporter]SCO1074 (14E5)_PCR/6451 [Gene]SCO1074/5876 NC_003888 SCG22.20, possible peptidase, len: 451 aa; similar to TR:Q9X7E4 (EMBL:AL049478) Mycobacterium leprae hypothetical 46.6 kD protein, 442 aa; fasta scores: opt: 1103 z-score: 1270.5 E(): 0; 43.8% identity in 443 aa overlap. Contains Pfam match to entry PF01546 Peptidase_M20, Peptidase family M20/M25/M40 1 4 19 8 [Reporter]SCO3744 (15A5)_PCR/6450 [Gene]SCO3744/5875 NC_003888 SCH22A.22, unknown, len: 261 aa 1 4 19 7 [Reporter]SCO0340 (16M1)_PCR/6449 [Gene]SCO0340/5874 NC_003888 SCF12.19c, unknown, len: 195aa; 1 4 19 6 [Reporter]SCO1596 (17I1)_PCR/6448 [Gene]SCO1596/5873 NC_003888 SCI35.18c, probable two-component sensor, len: 379 aa; similar to many e.g. HYDH_ECOLI sensor protein hydH (465 aa), fasta scores; opt: 296 z-score: 418.4 E(): 4.6e-16, 35.4% identity in 257 aa overlap. No apparent transmembrane domains. Contains Pfam match to entry PF00512 signal, Signal carboxyl-terminal domain, score 205.10, E-value 1e-57 1 4 19 5 [Reporter]SCO0640 (18E1)_PCR/6447 [Gene]SCO0640/5872 NC_003888 SCF56.24c, hypothetical protein, len: 142 aa; similar to various hypothetical proteins, e.g. TR:O86627 (EMBL:AL031155) Streptomyces coelicolor hypothetical 14.3 kD protein SC3A7.26, 129 aa; fasta scores: opt: 381 z-score: 485.9 E(): 1.1e-19; 55.4% identity in 121 aa overlap 1 3 2 14 [Reporter]SCO6865 (8E18)_PCR/4067 [Gene]SCO6865/3699 NC_003888 SC7F9.17c, possible regulatory protein, len: 402 aa. Weakly similar to a number of transcriptional regulatory proteins e.g. Pseudomonas stutzeri SW:NIRQ_PSEST (EMBL:Z17423) denitrification regulatory protein, NirQ (275 aa), fasta scores opt: 263 z-score: 268.0 E(): 1.6e-07 31.8% identity in 217 aa overlap. Contains a putative helix-turn-helix motif situated between residues 34..55 (+3.07 SD) and a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 1 4 19 4 [Reporter]SCO6114 (19A1)_PCR/6446 [Gene]SCO6114/5871 NC_003888 SC9B2.01c, partial CDS, possible secreted peptide binding /transport protein, len: 223aa; similar to many eg. SW:OPPA_BACSU oligopeptide-binding protein OppA from Bacillus subtilis (545 aa) fasta scores; opt: 117, z-score: 142.4, E(): 1.2, (25.9% identity in 139 aa overlap). Contains probable N-terminal signal sequence.,SCBAC33E8.02c, possible peptide-binding transport protein (fragment), len: >411 aa. Similar to C-terminal region of TR:Q9K6T2 (EMBL:AP001519) Bacillus halodurans dipeptide ABC transporter (dipeptide-binding protein) BH3644, 557 aa; fasta scores: opt: 199 Z-score: 227.0 bits: 51.4 E(): 4.8e-05; 23.179% identity in 302 aa overlap. Contains 2x Pfam matches to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5 1 3 2 13 [Reporter]SCO7141 (9A18)_PCR/4066 [Gene]SCO7141/3698 NC_003888 SC9A4.03, possible nitroreductase, len: 202 aa; similar to SW:NFNB_ECOLI (EMBL:D25414) Escherichia coli oxygen-insensitive NAD(P)H nitroreductase (dihydropteridine reductase) (EC 1.6.99.7) NfnB, 217 aa; fasta scores: opt: 136 z-score: 170.8 E(): 0.048; 28.4% identity in 169 aa overlap. Contains Pfam match to entry PF00881 Nitroreductase, Nitroreductase family 1 4 19 3 [Reporter]SCP1.315 (19M21)_PCR/6445 [Gene]SCP1.315/5870 NC_003888 SCP1.315, unknown, len: 139aa; previously sequenced as TR:Q9L8H2 (EMBL:AF204735). Strongly similar to TR:Q54403 (EMBL:Z11519) hypothetical protein from transposon Tn4811 of Streptomyces lividans (138 aa) fasta scores; opt: 858, z-score: 1093.1, E(): 0, 95.7% identity in 139 aa overlap. 1 3 2 12 [Reporter]SCO2329 (10M14)_PCR/4065 [Gene]SCO2329/3697 NC_003888 SCC53.20c, possible transcriptional regulator, len: 320aa; similar to many eg. TR:Q9S166 (EMBL:AB023785) AdpA transcriptional activator for StrR from Streptomyces griseus (405 aa) fasta scores; opt: 502, z-score: 592.7, E(): 1.5e-25, 32.6% identity in 319 aa overlap and TR:Q9X7Q2 (EMBL:AL049587) putative transcriptional regulator from Streptomyces coelicolor (325 aa) fasta scores; opt: 772, z-score: 909.9, E(): 0, 47.5% identity in 318 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family, Pfam match to entry PF01965 ThiJ, ThiJ/PfpI family and Prosite match to PS00041 Bacterial regulatory proteins, araC family signature. 1 3 2 11 [Reporter]SCO4496 (11I14)_PCR/4064 [Gene]SCO4496/3696 NC_003888 SCD35.03, possible acetyltransferase, len: 174 aa; similar to TR:CAB71250 (EMBL:AL138598) Streptomyces coelicolor putative acetyltransferase SC1A2.12, 181 aa; fasta scores: opt: 214 z-score: 278.1 E(): 4.6e-08; 32.9% identity in 149 aa overlap and to SW:TTR_PSESY (EMBL:X17150) Pseudomonas syringae (pv. syringae) acetyltransferase (EC 2.3.1.-) Ttr, 17 aa; fasta scores: opt: 137 z-score: 182.0 E(): 0.01; 28.3% identity in 138 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 3 2 10 [Reporter]SCO4244 (12E14)_PCR/4063 [Gene]SCO4244/3695 NC_003888 SCD8A.17c, unknown, len: 652 aa 1 3 2 9 [Reporter]SCO1528 (13A14)_PCR/4062 [Gene]SCO1528/3694 NC_003888 SCL2.18, possible elongation factor, len: 732 aa; similar to TR:O07170 (EMBL:Z96071) Mycobacterium tuberculosis FusA2, 714 aa; fasta scores: opt: 2328 z-score: 2349.0 E(): 0; 61.4% identity in 731 aa overlap, to SW:EFG (EMBL:X00415) Escherichia coli elongation factor G (EF-G) FusA, 703 aa; fasta scores: opt: 853 z-score: 862.2 E(): 0; 30.1% identity in 722 aa overlap and to SW:EFG2_STRCO (EMBL:AL031013) Streptomyces coelicolor elongation factor G 2 (EF-G 2), FusB, 686 aa; fasta scores: opt: 937 z-score: 947.0 E(): 0; 35.5% identity in 710 aa overlap. Contains Pfam matches to entries PF00009 GTP_EFTU, Elongation factor Tu family and PF00679 EFG_C, Elongation factor G C-terminus and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 2 8 [Reporter]SCO0331 (14M10)_PCR/4061 [Gene]SCO0331/3693 NC_003888 SCF12.10, possible short chain oxidoreductase (putative secreted protein), len: 299 aa; similar to many e.g. TR:CAB51136 (EMBL:Y18817) short chain oxidoreductase from actinorhodin biosynthesis cluster of Streptomyces coelicolor (276 aa) fasta scores; opt: 358, z-score: 403.3, E(): 4.1e-15, 34.6% identity in 280 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase 1 3 2 7 [Reporter]SCO3825 (15I10)_PCR/4060 [Gene]SCO3825/3692 NC_003888 SCGD3.26, probable ABC-transporter transmembrane component, len: 282 aa; may be involved in antibiotic resistance by acting as an efflux pump, highly similar to to many ABC-tranporter transmembrane components (especially those involved in antibiotic transport) e.g. TR:O86917 (EMBL:Y15759) Streptomyces rochei ABC-tranporter which induces multidrug resistance (284 aa), fasta scores; opt: 1491 z-score: 1785.3 E(): 0, 77.5% identity in 284 aa overlap. Similar to many from S.coelicolor e.g. (EMBL:AL049841), SCE9.24c, S.coelicolor probable ABC transporter transmembrane component (260 aa) (35.0% identity in 254 aa overlap). Contains hydrophobic, probable membrane-spanning regions. Contains PS00890 ABC-2 type transport system integral membrane proteins signature 1 3 2 6 [Reporter]SCO0373 (16E10)_PCR/4059 [Gene]SCO0373/3691 NC_003888 SCF41.32c, possible secreted protein, len: 199 aa. Contains a possible N-terminal signal sequence. 1 3 2 5 [Reporter]SCO1472 (17A10)_PCR/4058 [Gene]SCO1472/3690 NC_003888 SCL6.29c, conserved hypothetical Sun-family protein, len: 475 aa; similar to SW:SUN_BACSU (EMBL:Y13937) Bacillus subtilis sun protein, 447 aa; fasta scores: opt: 637 z-score: 711.7 E(): 3.2e-32; 32.0% identity in 472 aa overlap. Contains Pfam match to entry PF01029 NusB, NusB family and to entry PF01189 Nol1_Nop2_Sun, NOL1/NOP2/sun family and match to Prosite entry PS01153 NOL1/NOP2/sun family signature 1 4 19 2 [Reporter]SCO5095 (20E21)_PCR/6444 [Gene]SCO5095/5869 NC_003888 SCBAC28G1.21, ORF9, probable DNA-binding protein, len: 190 aa; identical to previously sequenced as TR:Q9X9V0 (EMBL:Y18817) Streptomyces coelicolor A3(2) hypothetical 20.6 kDa protein ORF9, 190 aa and similar to TR:O86784 (EMBL:AL031317) Streptomyces coelicolor SC6G4.21, 202 aa; fasta scores: opt: 642 Z-score: 656.2 bits: 128.2 E(): 7.9e-31; 53.968% identity in 189 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and possible helix-turn-helix motif at residues 25..46 (+6.01 SD) 1 4 18 22 [Reporter]SCO1020 (1E17)_PCR/6442 [Gene]SCO1020/5868 NC_003888 2SCG2.34c, conserved hypothetical protein, len: 440 aa; similar to the plasmid encoded SW:TERA_ALCSP (EMBL:M20238) Alcaligenes sp. tellurium resistance protein TerA, 339 aa; fasta scores: opt: 515 z-score: 412.2 E(): 1.9e-15; 35.1% identity in 396 aa overlap. Contains Pfam match to entry PF02342 TerD 1 4 18 21 [Reporter]SCO5585 (2A17)_PCR/6441 [Gene]SCO5585/5867 NC_003888 SC2E1.02, probable glnD, protein pII uridylyltransferase, len: 835 aa; similar to many e.g. GLND_ECOLI (890 aa), fasta scores; opt: 403 z-score: 540.4 E(): 7.3e-23, 26.2% identity in 851 aa overlap. Contains 7 degenerate repeats of the sequence PSS 1 4 18 20 [Reporter]SCO6798 (3M13)_PCR/6440 [Gene]SCO6798/5866 NC_003888 SC1A2.07, unknown, len: 191 aa. 1 4 18 19 [Reporter]SCO6207 (4I13)_PCR/6439 [Gene]SCO6207/5865 NC_003888 SC2G5.28, unknown, len: 84aa 1 4 18 18 [Reporter]SCO6378 (5E13)_PCR/6438 [Gene]SCO6378/5864 NC_003888 SC4A2.14c, small hydrophobic protein, len: 99 aa; contains segment of similarity to BCRC_BACLI bacitracin transport permease protein BcrC (203 aa), fasta scores; opt: 123 z-score: 260.1 E(): 3.1e-07, 60.6% identity in 33 aa overlap. Similar in parts to downstream ORF SC3A7.24c (57.0% identity in 114 aa overlap). Contains possible hydrophobic membrane spanning region 1 4 18 17 [Reporter]SCO6546 (6A13)_PCR/6437 [Gene]SCO6546/5863 NC_003888 SC5C7.31c, probable secreted cellulase, len: 973 aa; N-terminus is similar to the cellulose binding domain of e.g. GUN4_THEFU endoglucanase E-4 precursor (880 aa), fasta scores; opt: 341 z-score: 352.0 E(): 2.3e-12, 43.7% identity in 103 aa overlap. C-terminus is similar to many cellulases e.g. GUXB_CELFI exoglucanase B precursor (1090 aa), fasta scores; opt: 2997 z-score: 2688.8 E(): 0, 61.4% identity in 699 aa overlap. Contains probable N-terminal signal sequence and Pfam match to entry PF00553 CBD_1, Cellulose binding domain, score 122.30, E-value 2.4e-34 near N-terminus 1 4 18 16 [Reporter]SCO6778 (7M9)_PCR/6436 [Gene]SCO6778/5862 NC_003888 SC6A5.27c, probable transcriptional regulator, len: 228 aa; similar to many e.g. TR:O86514 (EMBL:AL031124) putative transcriptional regulator (194 aa) fasta scores; opt: 159, z-score: 206.2, E(): 0.00035, (28.5% identity in 151 aa overlap) and TR:Q54288 (EMBL:X86780) regulator of antibiotic transport complexes in Streptomyces hygroscopicus (204 aa) fasta scores; opt: 125, z-score: 163.8, E(): 0.08, (30.0% identity in 70 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and probable helix-turn-helix (+5.96 SD) 45-66aa. 1 3 2 4 [Reporter]SCO0688 (18M6)_PCR/4057 [Gene]SCO0688/3689 NC_003888 SCF15.09, unknown, len: 390 aa. Contains a probable coiled-coil between residues 285..329 (45 residues). 1 4 18 15 [Reporter]SCO2488 (8I9)_PCR/6435 [Gene]SCO2488/5861 NC_003888 SC7A8.27, nirC, probable nitrite reductase small subunit, len: 131 aa; similar to TR:O53675 (EMBL:AL021929) Mycobacterium tuberculosis nitrite reductase small subunit NirD, 118 aa; fasta scores: opt: 353 z-score: 455.1 E(): 5.9e-18; 56.5% identity in 92 aa overlap and to SW:NIRD_ECOLI (EMBL:X14202) Escherichia coli nitrite reductase [NAD(P)H] small subunit (EC 1.6.6.4) NirD, 108 aa; fasta scores: opt: 265 z-score: 346.0 E(): 7e-12; 46.9% identity in 96 aa overlap 1 3 2 3 [Reporter]SCO4061 (19I6)_PCR/4056 [Gene]SCO4061/3688 NC_003888 2SCD60.27c, unknown, len: 185 aa 1 4 18 14 [Reporter]SCO5497 (9E9)_PCR/6434 [Gene]SCO5497/5860 NC_003888 SC8D9.09, unknown, len: 164aa; similar to many of undefined function eg. TR:O06336 (EMBL:Z95390) hypothetical protein from Mycobacterium tuberculosis (177 aa) fasta scores; opt: 171, z-score: 213.4, E(): 0.00014, (34.9% identity in 109 aa overlap). 1 3 2 2 [Reporter]SCO5334 (20A6)_PCR/4055 [Gene]SCO5334/3687 NC_003888 SCBAC5H2.03c, unknown, len: 104 aa. Highly hydrophilic. High content in arginine and serine amino acid residues 1 3 1 22 [Reporter]SCO5185 (1A2)_PCR/4053 [Gene]SCO5185/3686 NC_003888 2SC3B6.09, possible peptidase, len: 470 aa; similar to TR:AAF73713 (EMBL:AE002246) Chlamydophila pneumoniae AR39 peptidase, M20/M25/M40 family CP0876, 439 aa; fasta scores: opt: 809 z-score: 938.0 E(): 0; 33.6% identity in 455 aa overlap. Contains Pfam match to entry PF01546 Peptidase_M20, Peptidase family M20/M25/M40 1 3 1 21 [Reporter]SCO4938 (1M22)_PCR/4052 [Gene]SCO4938/3685 NC_003888 SCK13.30, possible ECF-sigma factor, len: 304 aa; similar to TR:Q9RJS2 (EMBL:AL132707) Streptomyces coelicolor putative RNA polymerase sigma factor SCF51.13c, 298 aa; fasta scores: opt: 1110 z-score: 1299.2 E(): 0; 58.4% identity in 296 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) and possible helix-turn-helix motif at residues 144..165 (+2.59 SD) 1 3 1 20 [Reporter]SCO0219 (2I22)_PCR/4051 [Gene]SCO0219/3684 NC_003888 SCJ12.31, narI2, possible nitrate reductase gamma chain, len: 256 aa. Similar to many including: Streptomyces coelicolor TR:O86714 (EMBL:AL031515) putative nitrate reductase gamma chain NarI SC5C7.17c (240 aa), fasta scores: opt: 755 z-score: 891.5 E(): 0 46.8% identity in 235 aa overlap and Bacillus subtilis SW:NARI_BACSU (EMBL:Z49884) nitrate reductase gamma chain (EC 1.7.99.4) (223 aa), fasta scores: opt: 668 z-score: 790.4 E(): 0 43.6% identity in 225 aa overlap. Contains possible membrane spanning hydrophobic domains 1 3 1 19 [Reporter]SCO7393 (3E22)_PCR/4050 [Gene]SCO7393/3683 NC_003888 SC10G8.21c, possible lipoprotein, len: 249 aa. Weakly similar to Streptomyces coelicolor SW:YRJ1_STRCO(EMBL:L24552) hypothetical protein in RplJ 5'region (Orf 1) (fragment) (>256 aa), fasta scores opt: 120 z-score: 142.7 E(): 1.6 20.3% identity in 261 aa overlap. Contains a possible N-terminal signal sequence and an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 1 3 1 18 [Reporter]SCO6183 (4A22)_PCR/4049 [Gene]SCO6183/3682 NC_003888 SC2G5.04, possible transferase, len: 335aa; similar to many eg. TR:O05195 (EMBL:U70982) heptosyl transferase I from Pseudomonas aeruginosa (355 aa) a protein known to be involved in the synthesis of lipopolysaccharide, fasta scores; opt: 240, z-score: 250.8, E(): 1.1e-06, (29.3% identity in 324aa overlap). Contains Pfam match to entry PF01075 Heptosyltranf, Heptosyl transferase, score 35.10,E-value7.1e-09. 1 3 1 17 [Reporter]SCO6707 (5M18)_PCR/4048 [Gene]SCO6707/3681 NC_003888 SC4C6.17c, possible DNA ligase, len: 355 aa; similar to many archaeal and eukaryotic DNA ligases e.g. to the C-terminal half of SW:DNLI_ACIAM (EMBL:X63438), lig, Acidianus ambivalens DNA ligase (600 aa), fasta scores; opt: 240 z-score: 283.0 E(): 2e-08, 24.7% identity in 377 aa overlap. The majority of similar genes have an additional N-terminal region of 210-220 aa relative to this sequence. The putative DNA ligase TR:O28549 (EMBL:AE000984) from Archaeoglobus fulgidus is an exception (313 aa). Highest similarity is to TR:O69698 (EMBL:AL022121) Mycobacterium tuberculosis possible DNA ligase (358 aa) (60.4% identity in 361 aa overlap). Contains Pfam match to entry PF01068 DNA_ligase, DNA ligase and PS00697 ATP-dependent DNA ligase AMP-binding site 1 3 1 16 [Reporter]SCO2167 (6I18)_PCR/4047 [Gene]SCO2167/3680 NC_003888 SC5F7.34c, unknown, len: aa; 1 4 18 13 [Reporter]SCO7359 (10A9)_PCR/6433 [Gene]SCO7359/5859 NC_003888 SC9H11.13c, possible hydrolase, len: 251 aa. Weakly similar to several members of the Dienelactone hydrolase family e.g. Pseudomonas putida SW:CLCD_PSEPU(EMBL:M16964) carboxymethylenebutenolidase (EC 3.1.1.45) (246 aa), fasta scores opt: 265 z-score: 312.9 E(): 5.7e-10 27.1% identity in 255 aa overlap and Mycobacterium tuberculosis TR:O33307(EMBL:AL008967) hypothetical 26.2 kd protein (245 aa), fasta scores opt: 630 z-score: 731.0 E(): 0 43.1% identity in 246 aa overlap. Contains a Pfam match to entry PF01738 DLH, Dienelactone hydrolase family. 1 4 18 12 [Reporter]SCO4372 (11M5)_PCR/6432 [Gene]SCO4372/5858 NC_003888 SCD10.04c, unknown, len: 204 aa 1 4 18 11 [Reporter]SCO3943 (12I5)_PCR/6431 [Gene]SCO3943/5857 NC_003888 SCD78.10c, probable transcriptional regulator, len: 370 aa; identical to TR:G3644044 (EMBL:AF090833) putative transcriptional regulator (RstP) from Streptomyces coelicolor. Also similar to members of the lacI family of transcriptional regulators e.g. PURR_ECOLI ((EMBL:J04212)(fasta scores; opt: 345, z-score: 356.4, E(): 1.3e-12, 28.2% identity in 347 aa overlap) purine nucleotide synthesis repressor from Escherichia coli. Contains PS00356 Bacterial regulatory proteins, lacI family signature and Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family, score 13.70, E-value 2.3e-06 1 4 18 10 [Reporter]SCO3064 (13E5)_PCR/6430 [Gene]SCO3064/5856 NC_003888 SCE25.05, probable peptide transporter, len: 498 aa; similar to SW:DTPT_LACLA (EMBL:U05215) Lactococcus lactis di-/tripeptide transporter DtpT, 463 aa; fasta scores: opt: 1105 z-score: 1248.4 E(): 0; 38.2% identity in 468 aa overlap. Contains Pfam match to entry PF00854 PTR2, POT family and match to Prosite entry PS01023 PTR2 family proton/oligopeptide symporters signature 2 and possible hydrophobic membrane spanning regions 1 4 18 7 [Reporter]SCO0480 (16I1)_PCR/6427 [Gene]SCO0480/5855 NC_003888 SCF80.01, unknown, partial CDS, len: >193 aa. Similar in parts to Streptomyces coelicolor TR:CAB53333 (EMBL;AL109974) hypothetical 34.2 KD protein SCF34.22 (326 aa), fasta scores opt:opt: 296 z-score: 300.2 E(): 2.4e-09 40.5% identity in 148 aa overlap.,SCF76.20, unknown, partial CDS, len: >49 aa. 1 4 18 6 [Reporter]SCO3552 (17E1)_PCR/6426 [Gene]SCO3552/5854 NC_003888 SCH5.15c, putative membrane protein, len: 150 aa; similar to TR:O69623 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (125 aa) fasta scores; opt: 211, z-score: 258.8, E(): 4.1e-07, (39.6% identity in 111 aa overlap). Contains possible hydrophobic membrane spanning regions 1 4 18 5 [Reporter]SCO1468 (18A1)_PCR/6425 [Gene]SCO1468/5853 NC_003888 SCL6.25c, possible serine/threonine protein kinase, len: 774 aa; similar to TR:Q9ZBL8 (EMBL:AL035159) Mycobacterium leprae putative serine/threonine kinase MLCB1450.19c, 763 aa; fasta scores: opt: 682 z-score: 645.1 E(): 1.6e-28; 36.0% identity in 736 aa overlap and to SW:KMOS_MSVMO (EMBL:V01180) Moloney murine sarcoma virus MOS serine/threonine-protein kinase transforming protein (EC 2.7.1.-) V-MOS, 374 aa; fasta scores: opt: 230 z-score: 224.5 E(): 4.4e-05; 23.4% identity in 269 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain 1 4 18 4 [Reporter]SCP1.27c (18M21)_PCR/6424 [Gene]SCP1.27c/5852 NC_003888 SCP1.27c, unknown, len: 1123aa; 1 3 1 15 [Reporter]SCO2672 (7E18)_PCR/4046 [Gene]SCO2672/3679 NC_003888 SC6D10.15, possible membrane protein, len: 1113 aa; similar to TR:O86657 (EMBL:AL031182) Streptomyces coelicolor putative integral membrane protein (fragment) SC4A2.01c, 653 aa; fasta scores: opt: 220 z-score: 208.2 E(): 0.00035; 27.8% identity in 654 aa overlap. Contains possible N-terminal region signal peptide sequence and possible hydrophobic membrane spanning regions 1 4 18 3 [Reporter]SCO1660 (19I21)_PCR/6423 [Gene]SCO1660/5851 NC_003888 SCI52.02, glpK, glycerol kinase, len: 512 aa; similar to SW:GLPK_ECOLI (EMBL:X15054) Escherichia coli glycerol kinase (EC 2.7.1.30) GlpK or B3926, 501 aa; fasta scores: opt: 1756 Z-score: 2090.6 bits: 396.4 E(): 7.3e-109; 52.259% identity in 509 aa overlap and to TR:Q9RJM2 (EMBL:AL121849) Streptomyces coelicolor glycerol kinase GlpK2 or SCF6.05, 508 aa; fasta scores: opt: 1974 Z-score: 2055.1 bits: 389.8 E(): 9.3e-109; 58.020% identity in 505 aa overlap. Contains Pfam match to entry PF00370 FGGY, FGGY family of carbohydrate kinases and match to Prosite entry PS00445 FGGY family of carbohydrate kinases signature 2 1 3 1 14 [Reporter]SCO6860 (8A18)_PCR/4045 [Gene]SCO6860/3678 NC_003888 SC7F9.12c, unknown, len: 145 aa. 1 4 18 2 [Reporter]SCO7493 (20A21)_PCR/6422 [Gene]SCO7493/5850 NC_003888 SCBAC17A6.26, conserved hypothetical protein, len: 645 aa; similar to many e.g. TR:Q9K6N7 (EMBL:AP001519) BH3691 protein from Bacillus halodurans (702 aa) fasta scores: opt: 1188, Z-score: 1277.9, 34.802% identity (37.175% ungapped) in 658 aa overlap 1 3 1 13 [Reporter]SCO5853 (9M14)_PCR/4044 [Gene]SCO5853/3677 NC_003888 SC9B10.20c, unknown, len: 267 aa; similar to several hypothetical proteins eg.TR:Q55078 (EMBL:U50973) OrfA from Streptomyces sp. (275 aa), fasta scores; opt: 418 z-score: 665.7 E(): 6.4e-30, 37.1% identity in 267 aa overlap 1 3 1 12 [Reporter]SCO2325 (10I14)_PCR/4043 [Gene]SCO2325/3676 NC_003888 SCC53.16, possible integral membrane protein, len: 283aa; contains possible membrane-spanning hydrophobic regions. 1 3 1 11 [Reporter]SCO4102 (11E14)_PCR/4042 [Gene]SCO4102/3675 NC_003888 SCD17.06c, possible MerR family transcriptional regulator, len:319 aa. Similar to many other MerR family regulators e.g. Bradyrhizobium sp. (strain NC92) SW:NOLA_BRASN (EMBL; U33192) nodulation protein NolA (237 aa), fasta scores opt: 251 z-score: 286.3 E(): 1.4e-08 28.9% identity in 225 aa overlap. Contains a Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and a probable helix-turn-helix motif between residues 9..30 (+3.43 SD). 1 3 1 10 [Reporter]SCO4239 (12A14)_PCR/4041 [Gene]SCO4239/3674 NC_003888 SCD8A.12c, possible small membrane protein, len: 148 aa; similar to TR:P96484 (EMBL:Y08921) Streptomyces reticuli hypothetical 15.9 kD protein, MsiK, 152 aa; fasta scores: opt: 724 z-score: 885.9 E(): 0; 74.8% identity in 151 aa overlap. Contains possible hydrophobic membrane spanning region 1 3 1 9 [Reporter]SCO3118 (13M10)_PCR/4040 [Gene]SCO3118/3673 NC_003888 SCE41.27, unknown, len: 165 aa 4 1 6 14 [Reporter]SCO5954 (8H24)_PCR/1635 [Gene]SCO5954/1499 NC_003888 SC7H1.24, probable chitinase (putative secreted protein), len: 765 aa; similar to many eg. S. lividans CHIT_STRLI P36909 chitinase c precursor (ec 3.2.1.14) (619 aa), fasta scores; opt: 292 z-score: 779.5 E(): 0, 35.1% identity in 752 aa overlap. Contains PS01095 Chitinases family 18 active site signature, and possible N-terminal region signal peptide sequence 1 3 1 8 [Reporter]SCO3749 (14I10)_PCR/4039 [Gene]SCO3749/3672 NC_003888 SCH22A.27c, possible integral membrane protein, len: 216 aa. Contains possible hydrophobic membrane spanning regions 4 1 6 13 [Reporter]SCO6075 (9D24)_PCR/1634 [Gene]SCO6075/1498 NC_003888 SC9B1.22c, unknown, len: 273aa; similar to many hypothetical proteins eg. TR:O06629 (EMBL:Z95618) from Mycobacterium tuberculosis (318 aa) fasta scores; opt: 353, z-score: 408.1, E(): 2.1e-15, (32.5% identity in 252 aa overlap). 1 3 1 7 [Reporter]SCO0921 (15E10)_PCR/4038 [Gene]SCO0921/3671 NC_003888 SCM10.09c, hypothetical protein, len: 256 aa; similar to TR:O53274 (EMBL:AL021287) Mycobacterium tuberculosis hypothetical 27.0 kD protein, 246 aa; fasta scores: opt: 498 z-score: 593.9 E(): 1.1e-25; 47.7% identity in 237 aa overlap 4 1 6 12 [Reporter]SCO2749 (10P20)_PCR/1633 [Gene]SCO2749/1497 NC_003888 SCC57A.20, carbohydrate transport protein, len: 129 aa. Highly similar to Bacillus subtilis SW:RBSD_BACSU(EMBL:Z25798) high affinity ribose transport protein RbsD (131 aa), fasta scores opt: 379 z-score: 468.4 E(): 1.1e-18 44.3% identity in 131 aa overlap and Escherichia coli SW:RBSD_ECOLI(EMBL:L10328) high affinity ribose transport protein, RbsD (151 aa), fasta scores opt: 219 z-score: 276.4 E(): 5.2e-08 33.8% identity in 139 aa overlap. 1 3 1 6 [Reporter]SCO1612 (16A10)_PCR/4037 [Gene]SCO1612/3670 NC_003888 SCI35.34c, probable aldehyde dehydrogenase, len: 483 aa; similar to many e.g. DHAB_RHIME betaine aldehyde dehydrogenase (487 aa), fasta scores; opt: 1113 z-score: 1170.6 E(): 0, 42.4% identity in 462 aa overlap. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site, PS00070 Aldehyde dehydrogenases cysteine active site and Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenases, score 569.80, E-value 1.7e-167 4 1 6 11 [Reporter]SCO4657 (11L20)_PCR/1632 [Gene]SCO4657/1496 NC_003888 SCD40A.03c, possible integral membrane protein, len: 434 aa; similar to TR:Q9EWX4 (EMBL:AL445403) Streptomyces coelicolor putative integral membrane protein 2SCI34.05, 396 aa; fasta scores: opt: 678 Z-score: 732.5 E(): 3.6e-33; 37.923% identity in 414 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 6 10 [Reporter]SCO4309 (12H20)_PCR/1631 [Gene]SCO4309/1495 NC_003888 SCD95A.42, unknown, len: 126 aa 4 1 6 9 [Reporter]SCO1892 (13D20)_PCR/1630 [Gene]SCO1892/1494 NC_003888 SCI7.10, possible integral membrane efflux protein, len: 415aa; similar to many egs. TR:CAB41206 (EMBL:AL049661) putative integral membrane efflux protein from Streptomyces coelicolor (500 aa) fasta scores; opt: 347, z-score: 354.0, E(): 2.2e-12, (29.7% identity in 408 aa overlap) and SW:TCR3_ECOLI tetracycline resistance protein from Escherichia coli (396 aa) fasta scores; opt: 241, z-score: 249.6, E(): 1.4e-06, (25.9% identity in 398 aa overlap). Contains possible membrane spanning hydrophobic regions. 4 1 6 8 [Reporter]SCO0361 (14P16)_PCR/1629 [Gene]SCO0361/1493 NC_003888 SCF41.20c, unknown, len: 390 aa. Highly similar, over available sequence, to Caulobacter crescentus TR: Q46022 (EMBL; X98879) xylX gene (fragment) (>140 aa), fasta scores opt: 276 z-score: 326.5 E(): 7.8e-11 50.0% identity in 122 aa overlap. 4 1 6 7 [Reporter]SCO1837 (15L16)_PCR/1628 [Gene]SCO1837/1492 NC_003888 SCI8.22, unknown, len: 262 aa. 4 1 6 6 [Reporter]SCO0872 (16H16)_PCR/1627 [Gene]SCO0872/1491 NC_003888 SCM1.05c, unknown, len: 445 aa. Weakly similar to several Streptomyces coelicolor proteins including: TR:O86807(EMBL:AL031031) putative regulatory protein SC7C7.02C (916 aa), fasta scores opt: 306 z-score: 336.6 E(): 2.1e-11 30.7% identity in 264 aa overlap and TR:CAB59599(EMBL:AL132662) hypothetical regulatory protein SCF11.22 (877 aa), fasta scores opt: 284 z-score: 312.7 E(): 4.5e-10 34.6% identity in 243 aa overlap. Contains a Pfam match to entry PF00072 response_reg, Response regulator receiver domain. 4 1 6 5 [Reporter]SCO1683 (17D16)_PCR/1626 [Gene]SCO1683/1490 NC_003888 SCI30A.04c, possible amino acid permease, len: 512 aa; similar to many e.g. TR:Q53148 (EMBL:L24492) ethanolamine permease from Rhodococcus sp. strain NI86/21 (488 aa) fasta scores; opt: 266, z-score: 293.8, E(): 4.9e-09, (26.7% identity in 397 aa overlap). Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease. Contains possible membrane spanning hydrophobic regions. 1 4 17 22 [Reporter]SCO1016 (1A17)_PCR/6420 [Gene]SCO1016/5849 NC_003888 2SCG2.30c, possible secreted protein, len: 274 aa. Contains possible N-terminal region signal peptide sequence. Rich in alanine and proline amino acid residues. High content in G+C (79.8%) 1 4 17 21 [Reporter]SCO1000 (2M13)_PCR/6419 [Gene]SCO1000/5848 NC_003888 2SCG2.13c, integrase, len: 370 aa; N-terminal previously sequenced and annotated as TR:Q9X468 (EMBL:AF099015) Streptomyces coelicolor integrase Int, 222 aa. Contains Pfam match to entry PF00589 Phage_integrase, Phage integrase family 1 4 17 20 [Reporter]SCO6794 (3I13)_PCR/6418 [Gene]SCO6794/5847 NC_003888 SC1A2.03, cvnA7, possible membrane protein, len: 650 aa. Weakly similar to several including Streptomyces coelicolor TR:Q9X836(EMBL:AL049727) putative large secreted protein, SC9B1.16C (877 aa), fasta scores opt: 781 z-score: 616.3 E(): 6.4e-27 35.1% identity in 582 aa overlap and Streptomyces coelicolor TR:CAB61282(EMBL:AL132991) putative sensor kinase, SCF55.12C (575 aa), fasta scores opt: 426 z-score: 343.4 E(): 1e-11 28.9% identity in 508 aa overlap. Contains two possible membrane spanning hydrophobic domains towards the N-terminal and 4x VPAPV repeat between residues 485..504. 1 4 17 19 [Reporter]SCO5476 (4E13)_PCR/6417 [Gene]SCO5476/5846 NC_003888 SC2A11.10, probable oligopeptide transport integral membrane protein, len: 335 aa; similar to mnay e.g. APPC_BACSU oligopeptide transport permease protein appC (303 aa), fasta scores; opt: 465 z-score: 1038.5 E(): 0, 41.4% identity in 309 aa overlap. Also similar to S. coelicolor TR:P72406 (EMBL:U68036) bldKA (335 aa), fasta scores; opt: 912 z-score: 1192.3 E(): 0, 42.1% identity in 330 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature and Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 47.00, E-value 4.1e-10 1 4 17 18 [Reporter]SCO6374 (5A13)_PCR/6416 [Gene]SCO6374/5845 NC_003888 SC4A2.10c, probable sugar transferase, len: 478; similar to many bacterial hypothetical proteins and to TR:O21943 (EMBL:AF021347) bactoprenol glucosyl transferase from bacteriophage SFII (309 aa), fasta scores; opt: 809 z-score: 925.5 E(): 0, 40.9% identity in 303 aa overlap. Contains a C-terminal extension with four additional transmembrane domains not present in similar proteins and Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases, score 46.50, E-value 6.1e-10 1 4 17 17 [Reporter]SCO7458 (6M9)_PCR/6415 [Gene]SCO7458/5844 NC_003888 SC5C11.15, possible membrane protein, len: 121 aa. Similar in parts to several proteins of undefined function e.g. Mycobacterium tuberculosis TR:O53879(EMBL:AL022004) hypothetical 40.0 KD protein (407 aa), fasta scores opt: 331 z-score: 403.1 E(): 4.9e-15 50.0% identity in 108 aa overlap. Contains a possible membrane spanning hydrophobic domain. 1 4 17 15 [Reporter]SCO5318 (8E9)_PCR/6413 [Gene]SCO5318/5843 NC_003888 SC6G9.15, polyketide beta-ketoacyl synthase alpha, len: 423 aa; whiE locus polyketide beta-ketoacyl synthase alpha previously sequenced from S. coelicolor as SW:KAS1_STRCO (EMBL:X55942). May form heterodimer with SW:KAS2_STRCO, SC6G9.16 beta-ketoacyl synthase beta. Similar to others from S.coelicolor e.g. TR:O54149 (EMBL:AL021409) probable 3-oxoacyl-[acyl-carrier-protein] synthase (407 aa) (39.1% identity in 419 aa overlap). Contains Pfam match to entry PF00109 ketoacyl-synt, Beta-ketoacyl synthase and PS00606 Beta-ketoacyl synthases active site 1 4 17 14 [Reporter]SCO5493 (9A9)_PCR/6412 [Gene]SCO5493/5842 NC_003888 SC8D9.05, unknown, len: 331aa; similar to others of undefined function eg. TR:O53262 (EMBL:AL021287) hypothetical protein from Mycobacterium tuberculosis (337 aa) opt: 774, z-score: 895.1, E(): 0, (43.4% identity in 334 aa overlap). 1 3 1 5 [Reporter]SCO0107 (17M6)_PCR/4036 [Gene]SCO0107/3669 NC_003888 SCJ11.36c, possible noncomposite drug resistance transposon aminoglycoside nucleotidyltransferase, len: 185 aa; some similarity to many involved in antibiotic resistance e.g. TR:Q53702 (EMBL:J03947) lincosaminide nucleotidyltransferase from Staphylococcus aureus (161 aa) fasta scores; opt: 176, z-score: 229.0, E(): 2.1e-05, (28.4% identity in 148 aa overlap) 1 4 17 13 [Reporter]SCO2791 (10M5)_PCR/6411 [Gene]SCO2791/5841 NC_003888 SCC105.22c, unknown, len: 103 aa 1 3 1 4 [Reporter]SCO0437 (18I6)_PCR/4035 [Gene]SCO0437/3668 NC_003888 SCF51A.15c, hypothetical protein, len: 341 aa; similar to TR:Q9A8Z7 (EMBL:AE005797) Caulobacter crescentus hypothetical protein CC1198, 331 aa; fasta scores: opt: 855 Z-score: 977.0 E(): 8.7e-47; 45.181% identity in 332 aa overlap 1 4 17 12 [Reporter]SCO5284 (11I5)_PCR/6410 [Gene]SCO5284/5840 NC_003888 SCCB12.08c, hypothetical protein, len: 235 aa; similar to TR:Q9RVH7 (EMBL:AE001956) Deinococcus radiodurans hypothetical 24.5 kDa protein DR1052, 230 aa; fasta scores: opt: 403 z-score: 466.7 E(): 1.6e-18; 38.5% identity in 218 aa overlap and to SW:SPEE_ECOLI (EMBL:J02804) Escherichia coli spermidine synthase (EC 2.5.1.16) SpeE, 287 aa; fasta scores: opt: 173 z-score: 204.7 E(): 0.00063; 31.9% identity in 119 aa overlap 1 3 1 3 [Reporter]SCP1.296 (19E6)_PCR/4034 [Gene]SCP1.296/3667 NC_003888 SCP1.296, unknown, len: 179aa; 4 2 23 22 [Reporter]SCO4930 (1L23)_PCR/4032 [Gene]SCO4930/3666 NC_003888 SCK13.22, probable enoyl-CoA hydratase, len: 266 aa; similar to SW:PAAF_ECOLI (EMBL:AE000236) Escherichia coli probable enoyl-CoA hydratase PaaF (EC 4.2.1.17), 255 aa; fasta scores: opt: 562 z-score: 664.6 E(): 1.8e-29; 40.2% identity in 259 aa overlap. Contains Pfam match to entry PF00378 ECH, Enoyl-CoA hydratase/isomerase family and match to Prosite entry PS00166 Enoyl-CoA hydratase/isomerase signature 4 2 23 21 [Reporter]SCO3202 (2H23)_PCR/4031 [Gene]SCO3202/3665 NC_003888 SCE22.19c, hrdD, RNA polymerase principal sigma factor, len: 332 aa; identical to previously sequenced SW:HRDD_STRCO (EMBL:X52982) Streptomyces coelicolor RNA polymerase principal sigma factor HrdD, 332 aa. Contains Pfam match to entry PF00140 sigma70, Sigma-70 factor and matches to Prosite entries PS00715 Sigma-70 factors family signature 1 and PS00716 Sigma-70 factors family signature 2. Contains also possible helix-turn-helix motif at residues 292..313 (+5.89 SD) 4 2 23 20 [Reporter]SCO7400 (3D23)_PCR/4030 [Gene]SCO7400/3664 NC_003888 SC10G8.28c, possible ABC-transport protein, ATP-binding component, len: 292 aa. Highly similar to many e.g. Escherichia coli SW:FEPC_ECOLI(EMBL:X57471) ferric enterobactin transport ATP-binding protein, FepC (271 aa), fasta scores opt: 670 z-score: 720.4 E(): 1e-32 41.7% identity in 266 aa overlap and Escherichia coli SW:FHUC_ECOLI(EMBL:X05810) ferrichrome transport ATP-binding protein, FhuC (265 aa), fasta scores opt: 569 z-score: 613.5 E(): 9.2e-27 39.2% identity in 240 aa overlap. Also similar to Streptomyces coelicolor TR:CAB53325(EMBL:AL109974) putative iron-siderophore ABC-transporter, ATP-binding protein, SCF34.14c (287 aa), fasta scores opt: 728 z-score: 571.3 E(): 2.3e-26 46.7% identity in 274 aa overlap. Contains Prosite hits to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Also contains a Pfam match to entry PF00005 ABC_tran, ABC transporter. 4 2 23 19 [Reporter]SCO7624 (4P19)_PCR/4029 [Gene]SCO7624/3663 NC_003888 SC2H2.22c, possible tetR-family regulatory protein, len: 231 aa; similar to many other putative tetR-family transcriptional regulators, e.g. TR:CAC08314 (EMBL:AL392149) Streptomyces coelicolor putative tetR-family transcriptional regulatory protein SCD19.13c, 268 aa; fasta scores: opt: 456 z-score: 539.8 E(): 1.6e-22; 38.4% identity in 232 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 4 1 6 4 [Reporter]SCO0392 (18P12)_PCR/1625 [Gene]SCO0392/1489 NC_003888 SCF62.18, possible methyltransferase, len: 416 aa. Similar to several including: Streptomyces peucetius TR:O68501 (EMBL; AF048833) daunorubicin/doxorubicin biosynthesis enzyme DnrX (403 aa), fasta scores opt: 801 z-score: 923.6 E(): 0; 36.7% identity in 403 aa overlap and Streptomyces fradiae TR:AAD41823 (EMBL:AF147704) NDP-hexose 3-C-methyltransferase TYLCIII (418 aa), fasta scores opt: 688 z-score: 793.6 E(): 0; 34.1% identity in 425 aa overlap 4 2 23 18 [Reporter]SCO6714 (5L19)_PCR/4028 [Gene]SCO6714/3662 NC_003888 SC4C6.24c, possible hydroxylase, len: 263 aa; similar to TR:Q55078 (EMBL:U50973) Streptomyces sp. strain C5 daunomycin C-14 hydroxylase (275 aa), fasta scores; opt: 422 z-score: 482.3 E(): 1.5e-19, 38.5% identity in 273 aa overlap. Similar to TR:O50527 (EMBL:AL009204) S.coelicolor hypothetical protein (267 aa) (37.9% identity in 253 aa overlap) and to (EMBL:AL049863), SC5H1.09c, S.coelicolor possible hydroxylase (265 aa) (35.7% identity in 258 aa overlap). Also similar to hypothetical proteins from Mycobacterium tuberculosis and to Mycobacterium tuberculosis 27.3 kDa MAb HBT7 reactive antigen (EMBL:AJ007737) (260 aa) (36.7% identity in 264 aa overlap) 4 1 6 3 [Reporter]SCO1312 (19L12)_PCR/1624 [Gene]SCO1312/1488 NC_003888 SCBAC36F5.23c, probable transcriptional regulatory protein, len: 201 aa; similar to TR:Q9I1G3 (EMBL:AE004657) Pseudomonas aeruginosa probable transcriptional regulator PA2312, 193 aa; fasta scores: opt: 339 Z-score: 397.1 bits: 80.3 E(): 1.6e-14; 35.519% identity in 183 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and two possible helix-turn-helix domains at residues 35..56 (+2.92 SD) and 64..85 (+4.24 SD) 4 2 23 17 [Reporter]SCO7566 (6H19)_PCR/4027 [Gene]SCO7566/3661 NC_003888 SC5F1.20, probable oligopeptide ABC transporter ATP-binding protein, len: 288 aa; similar to SW:OPPD_BACSU (EMBL:X56347) Bacillus subtilis oligopeptide transport ATP-binding protein OppD, 358 aa; fasta scores: opt: 934 z-score: 1022.9 E(): 0; 54.2% identity in 262 aa overlap and to TR:CAC05750 (EMBL:AL391751) Streptomyces coelicolor putative peptide transport system ATP-binding protein SC9E12.05, 327 aa; fasta scores: opt: 905 z-score: 714.2 E(): 4e-34; 53.5% identity in 260 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 1 6 2 [Reporter]SCO6299 (20D12)_PCR/1623 [Gene]SCO6299/1487 NC_003888 SCIF3.01c, probable tetR-family transcriptional regulator, len: >248 aa; similar to TR:Q9XDV7 (EMBL:AB023642) Streptomyces griseus DNA-binding protein ORF2, 289 aa; fasta scores: opt: 847 z-score: 994.6 E(): 0; 65.3% identity in 245 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 28..49 (+3.30 SD),SCBAC8D1.12c, possible DNA-binding protein (fragment), len: >72 aa; similar to TR:Q9XDV7 (EMBL:AB023642) Streptomyces griseus DNA-binding protein ORF2, 289 aa; fasta scores: opt: 247 Z-score: 319.9 bits: 65.1 E(): 3.1e-10; 60.274% identity in 73 aa overlap 4 2 23 16 [Reporter]SCO2679 (7D19)_PCR/4026 [Gene]SCO2679/3660 NC_003888 SC6D10.22c, possible lipoprotein, len: 123 aa; similar to TR:O86703 (EMBL:AL031515) Streptomyces coelicolor putative secreted protein SC5C7.06, 318 aa; fasta scores: opt: 99 z-score: 130.8 E(): 7.1; 29.8% identity in 94 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 1 5 22 [Reporter]SCO5252 (1D8)_PCR/1621 [Gene]SCO5252/1486 NC_003888 2SC7G11.14, hypothetical protein, len: 194 aa; previously sequenced as TR:Q9RGW3 (EMBL:AF104994) Streptomyces coelicolor A3(2) hypothetical 18.8 kDa protein, 169 aa 4 1 5 21 [Reporter]SCO5003 (2P4)_PCR/1620 [Gene]SCO5003/1485 NC_003888 SCK15.05c, chiA, chinitase A precursor, len: 571 aa; identical to previously sequenced TR:Q9Z9M9 (EMBL:AB017008) Streptomyces coelicolor chinitase A precursor ChiA, 571 aa and to TR:Q59924 (EMBL:D13775) Streptomyces lividans chitinase A precursor (EC 3.2.1.14) ChiA, 571 aa. Contains Pfam match to entry PF02018 CBD_6, Cellulose binding domain, PF00041 fn3, Fibronectin type III domain, PF00704 Glyco_hydro_18, Glycosyl hydrolases family 18 and matches to Prosite entries PS00678 Trp-Asp (WD) repeats signature and PS01095 Chitinases family 18 active site. Contains also possible N-terminal region signal peptide sequence 4 1 5 20 [Reporter]SCO4718 (3L4)_PCR/1619 [Gene]SCO4718/1484 NC_003888 SCD31.43, rplR, 50S ribosomal protein L18, len: 127 aa; identical to previously sequenced SW:RL18_STRCO (EMBL:X83011) Streptomyces coelicolor 50S ribosomal protein L18 RplR, 127 aa. Contains Pfam match to entry PF00861 Ribosomal_L18p, Ribosomal L18p/L5e family and match to Prosite entry PS00358 Ribosomal protein L5 signature 4 1 5 19 [Reporter]SCO6918 (4H4)_PCR/1618 [Gene]SCO6918/1483 NC_003888 SC1B2.24c, unknown, len: 580 aa. 4 1 5 18 [Reporter]SCO6356 (5D4)_PCR/1617 [Gene]SCO6356/1482 NC_003888 SC3A7.24c, probable integral membrane protein, len: 201 aa; some similarity to e.g. BCRC_BACLI bacitracin transport permease protein bcrC (203 aa), fasta scores; opt: 203 z-score: 285.3 E(): 1.2e-08, 29.3% identity in 147 aa overlap 4 1 5 17 [Reporter]SCO7327 (5P24)_PCR/1616 [Gene]SCO7327/1481 NC_003888 SC4G10.06c, possible two-component system sensory histidine kinase, len: 1331 aa. Highly similar in parts to many including: Pseudomonas syringae (pv. syringae) SW:LEMA_PSESY(EMBL:M80477) sensor protein, LemA (EC 2.7.3.-), (907 aa), fasta scores opt: 641 z-score: 627.6 E(): 1.7e-27 31.2% identity in 872 aa overlap and Streptomyces coelicolor TR:O86808(EMBL:AL031031) putative sensory histidine kinase, SC7C7.03 (1829 aa), fasta scores opt: 2600 z-score: 2540.8 E():0 59.1% identity in 1346 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain, PF00512 signal, Histidine kinase and Pfam match to entry PF01590 GAF, GAF domain. Contains a 5x degenerate repeat region between residues 55..493 and a possible coiled-coil between residues 701..756. 4 1 5 16 [Reporter]SCO4853 (6L24)_PCR/1615 [Gene]SCO4853/1480 NC_003888 SC5G8.21c, unknown, len: 136 aa; weakly similar to other hypothetical proteins eg. TR:AAF12497 (EMBL:AE001863) from Deinococcus radiodurans (117 aa) fasta scores; opt: 109, z-score: 149.3, E(): 0.74, 26.1% identity in 111 aa overlap. 1 4 17 11 [Reporter]SCO4796 (12E5)_PCR/6409 [Gene]SCO4796/5839 NC_003888 SCD63A.07c, possible NLP/P60 family secreted protein, len: 398 aa; similar to TR:Q9XAQ3 (EMBL:AL078618) Streptomyces coelicolor putative NLP/P60 family secreted protein SCD16A.22, 277 aa; fasta scores: opt: 479 z-score: 430.6 E(): 1.6e-16; 39.1% identity in 233 aa overlap, to TR:Q9X7Z5 (EMBL:AL049497) Streptomyces coelicolor putative secreted protein SC6G10.09c, 347 aa; fasta scores: opt: 576 z-score: 513.3 E(): 4e-21; 41.5% identity in 395 aa overlap and to Streptomyces coelicolor SCD63A.04c, 347 aa. Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family. Contains also possible coiled-coil regions at aprox. residues 58..98 and 176..242 and possible N-terminal signal peptide sequence 1 4 17 10 [Reporter]SCO3206 (13A5)_PCR/6408 [Gene]SCO3206/5838 NC_003888 SCE22.23c, probable transmembrane efflux protein, len: 514 aa; similar to SW:PUR8_STRLP (EMBL:X76855) Streptomyces lipmanii puromycin resistance protein Pur8, 503 aa; fasta scores: opt: 1634 z-score: 1768.4 E(): 0; 53.1% identity in 493 aa overlap and to many other efflux proteins, e.g. TR:CAA18346 (EMBL:AL022268) Streptomyces coelicolor transmembrane efflux protein SC4H2.31c, 515 aa; fasta scores: opt: 1237 z-score: 1139.7 E(): 0; 41.0% identity in 493 aa overlap. Contains match to Prosite entry PS00216 Sugar transport proteins signature 1 and possible hydrophobic membrane spanning regions 1 4 17 9 [Reporter]SCO5045 (14M1)_PCR/6407 [Gene]SCO5045/5837 NC_003888 SCK7.18, conserved hypothetical protein, len: 227 aa; similar to TR:CAB88834 (EMBL:AL353832) Streptomyces coelicolor hypothetical 30.8 kDa protein SCE6.30c, 277 aa; fasta scores: opt: 403 z-score: 468.8 E(): 1.2e-18; 38.6% identity in 220 aa overlap 1 4 17 8 [Reporter]SCO0931 (15I1)_PCR/6406 [Gene]SCO0931/5836 NC_003888 SCM10.19, possible secreted proline-rich protein, len: 203 aa. Contains possible N-terminal signal peptide sequence and proline-rich region (giving similarities to proline-rich regions in many other proteins) and transmembrane domain at C-terminal region 1 4 17 7 [Reporter]SCO0240 (16E1)_PCR/6405 [Gene]SCO0240/5835 NC_003888 SCJ9A.19c, possible oxidoreductase, len: 311aa; similar to many proposed oxidoreductases eg. TR:O53613 (EMBL:AL021428) putative oxidoreductase from Mycobacterium tuberculosis (303 aa) fasta scores; opt: 746, z-score: 814.7, E(): 0, (43.0% identity in 305 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase 1 4 17 6 [Reporter]SCO0629 (17A1)_PCR/6404 [Gene]SCO0629/5834 NC_003888 SCF56.13c, possible transcriptional regulator, len: 369 aa; similar to TR:Q9Z509 (EMBL:AL035591) Streptomyces coelicolor putative transcriptional regulator (LacI family) SCC54.16, 368 aa; fasta scores: opt: 367 z-score: 401.8 E(): 5.4e-15; 33.2% identity in 352 aa overlap and to SW:CCPA_BACME (EMBL:L26052) Bacillus megaterium glucose-resistance amylase regulator CcpA, 332 aa; fasta scores: opt: 263 z-score: 290.5 E(): 8.4e-09; 26.3% identity in 339 aa overlap. Contains possible helix-turn-helix motif at residuees 25..46 (+5.42 SD) 1 4 17 5 [Reporter]SCO0132 (17M21)_PCR/6403 [Gene]SCO0132/5833 NC_003888 SCJ21.13, probable transcriptional regulator, len: 919 aa; similar to many e.g. TR:AAD28307 (EMBL:U95170) Rhodococcus erythropolis ThcG (927 aa), fasta scores; opt: 1176 z-score: 1258.4 E(): 0, 34.2% identity in 933 aa overlap, and TR:AAA53487 (EMBL:U03114) Streptomyces albus LipR (890 aa), fasta scores; opt: 740 z-score: 791.4 E(): 0, 31.1% identity in 952 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop), Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, and probable helix-turn-helix motif at aa 871-892 (Score 1389, +3.92 SD) 1 4 17 4 [Reporter]SCO4059 (18I21)_PCR/6402 [Gene]SCO4059/5832 NC_003888 2SCD60.25, possible transcriptional regulator, len: 111 aa; similar to TR:O86818 (EMBL:AL031031) Streptomyces coelicolor putative transcriptional regulator SC7C7.13, 124 aa; fasta scores: opt: 200 z-score: 263.0 E(): 4.4e-07; 43.3% identity in 97 aa overlap. Contains Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family and possible helix-turn-helix motif at residues 30..51 (+4.25 SD) 4 2 23 15 [Reporter]SCO2005 (8P15)_PCR/4025 [Gene]SCO2005/3659 NC_003888 SC7H2.19, unknown, len: 179aa; similar to many of undefined function egs. SW:YBDB_ECOLI hypothetical protein from Escherichia coli (137 aa) fasta scores; opt: 339, z-score: 407.0, E(): 2.5e-15, (50.0% identity in 116 aa overlap) and SW:CMA2_BACSU ComA protein, involved in competence (126 aa) fasta scores; opt: 312, z-score: 376.3, E(): 1.3e-13, (44.5% identity in 110 aa overlap). 1 4 17 3 [Reporter]SCP1.13c (19E21)_PCR/6401 [Gene]SCP1.13c/5831 NC_003888 SCP1.13c, conserved hypothetical protein, len: 538aa; C-terminal similar to TR:Q9KXL1 (EMBL:AL357524) hypothetical protein from Streptomyces coelicolor (282 aa) fasta scores; opt: 682, z-score: 810.6, E(): 0, 51.1% identity in 235 aa overlap. Contains TTA encoded leucine at residue 154, possible target for bldA regulation. 4 2 23 14 [Reporter]SCO6115 (9L15)_PCR/4024 [Gene]SCO6115/3658 NC_003888 SC9B2.02, possible ROK family transcriptional regulator, len: 407 aa; similar to many e.g. SW:XYLR_BACSU xylose repressor XylR from Bacillus subtilis (384 aa) fasta scores; opt: 470, z-score: 522.8, E(): 7.9e-22, (28.4% identity in 370 aa overlap). Contains Pfam match to entry PF00480 ROK, ROK family. Also contains a probable helix-turn-helix (+3.71 SD) at residues 32 to 53. 1 4 17 2 [Reporter]SCO5080 (20M17)_PCR/6400 [Gene]SCO5080/5830 NC_003888 SCBAC28G1.06, actVA5, possible hydrolase, len: 381 aa; identical to previously sequenced TR:Q53907 (EMBL:X58833) Streptomyces coelicolor 6 ActVA region genes of the actinorhodin biosynthetic gene cluster, ActVA5, 381 aa and similar to TR:Q9F0J3 (EMBL:AF218066) Streptomyces arenae hydrolase NcnH, 405 aa; fasta scores: opt: 1391 Z-score: 1521.5 bits: 290.3 E(): 3.9e-77; 56.522% identity in 368 aa overlap 4 2 23 13 [Reporter]SCO2437 (10H15)_PCR/4023 [Gene]SCO2437/3657 NC_003888 SCC24.08, possible integral membrane protein, len: 403 aa. Contains possible hydrophobic membrane spanning regions 4 2 23 12 [Reporter]SCO4109 (11D15)_PCR/4022 [Gene]SCO4109/3656 NC_003888 SCD17.13, possible oxidoreductase, partial CDS, len: >136 aa. Similar to several including: Streptomyces fradiae TR:AAD41821 (EMBL; AF147704) NDP-Hexose 2,3-Enoyl reductase TYLCII (329 aa), fasta scores opt: 251 z-score: 325.2 E(): 9.3e-11 41.9% identity in 124 aa overlap and Saccharopolyspora erythraea (Streptomyces erythraeus) TR:O33936 (EMBL; U77454)EryBII (333 aa), fasta scores opt: 245 z-score: 317.5 E(): 2.5e-10 39.3% identity in 135 aa overlap.,SCD17A.01, possible oxidoreductase (fragment), len: >218 aa; similar to TR:Q9RJC4 (EMBL:AL133171) Streptomyces coelicolor putative aldo/keto reductase (fragment) SCF81.28c, >307 aa; fasta scores: opt: 445 z-score: 528.2 E(): 6.5e-22; 39.7% identity in 219 aa overlap. Contains 2x Pfam matches to entry PF00248 aldo_ket_red, Aldo/keto reductase family 4 2 23 11 [Reporter]SCO4163 (12P11)_PCR/4021 [Gene]SCO4163/3655 NC_003888 SCD84.30, possible secreted protein, len: 245 aa. Contains possible N-terminal region signal peptide sequence 4 2 23 10 [Reporter]SCO3446 (13L11)_PCR/4020 [Gene]SCO3446/3654 NC_003888 SCE46.03c, possible dehalogenase, len: 225 aa; similar to SW:HAD_PSESP (EMBL:S74078) Pseudomonas sp. (strain YL) 2-haloalkanoic acid dehalogenase (EC 3.8.1.2), 232 aa; fasta scores: opt: 243 z-score: 288.1 E(): 1.1e-08; 23.5% identity in 217 aa overlap. Contins match to Pfam entry PF00702 Hydrolase, haloacid dehalogenase-like hydrolase 4 2 23 9 [Reporter]SCO1900 (14H11)_PCR/4019 [Gene]SCO1900/3653 NC_003888 SCI7.18, possible integral membrane sugar transport protein, len: 292aa; similar to many eg. TR:O30833 (EMBL:AF018073) sorbitol/mannitol transport inner membrane protein from Rhodobacter sphaeroides (276 aa) fasta scores; opt: 659, z-score: 770.4, E(): 0, (40.6% identity in 276 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Also contains possible membrane spanning hydrophobic regions. 4 1 5 15 [Reporter]SCO1427 (7H24)_PCR/1614 [Gene]SCO1427/1479 NC_003888 SC6D7.12c, unknown, len: 105 aa. 4 2 23 8 [Reporter]SCO0001 (15D11)_PCR/4018 [Gene]SCO0001/3652 NC_003888 SCEND.02c, unknown, doubtful CDS, len: 225aa; 4 1 5 14 [Reporter]SCO5950 (8D24)_PCR/1613 [Gene]SCO5950/1478 NC_003888 SC7H1.20c. probable export protein, len: 483; similar to many members of the drug resistance translocase family eg. Streptomyces lipmanii PUR8_STRLP P42670 puromycin resistance protein pur8 (503 aa), fasta scores; opt: 907 z-score: 636.6 E(): 2.9e-28, 38.6% identity in 477 aa overlap. Also weakly similar to upstream protein SC7H1.27 (533 aa) (E(): 7.9e-15, 28.6% identity in 461 aa overlap) 4 2 23 7 [Reporter]SCO1066 (16P7)_PCR/4017 [Gene]SCO1066/3651 NC_003888 SCG22.12, probable lacI-family transcriptional regulator, len: 340 aa; similar to SW:CYTR_ECOLI (EMBL:X03683) Escherichia coli transcriptional repressor CytR, 341 aa; fasta scores: opt: 608 z-score: 692.2 E(): 4.4e-31; 34.2% identity in 339 aa overlap. Contains Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and match to Prosite entry PS00356 Bacterial regulatory proteins, lacI family signature and possible helix-turn-helix motif at residues 4..25 (+6.04 SD) 4 1 5 13 [Reporter]SCO6459 (9P20)_PCR/1612 [Gene]SCO6459/1477 NC_003888 SC9B5.26c, probable regulatory protein, len: 120aa; similar to many eg. TR:O69711 (EMBL:AL022121) putative regulatory protein from Mycobacterium tuberculosis (120 aa) fasta scores: opt: 480, z-score: 880.6, E(): 0, (63.2% identity in 117 aa overlap) and TR:O54057 (EMBL:AJ001934) nolR, negative regulator of nodulation genes in Rhizobium leguminosarum bv. viciae strain TOM (105 aa) fasta scores: opt: 156, z-score: 262.6, E(): 2.4e-07, (34.4% identity in 93 aa overlap). Contains Pfam match to entry PF01022 HTH_ARSR_family, Bacterial regulatory protein, arsR family corresponding to a probable helix-turn-helix at residues 51 to 72 (+5.18 SD). 4 2 23 6 [Reporter]SCO0467 (17L7)_PCR/4016 [Gene]SCO0467/3650 NC_003888 SCF76.07, unknown, len: 247 aa; similar to TR:Q98LL0 (EMBL:AP002996) MLR0982 protein from Rhizobium loti (Mesorhizobium loti) (215 aa) fasta scores; opt: 410, Z-score: 484.9, E(): 2.2e-19, 33.178% identity (33.649% ungapped) in 214 aa overlap. 4 1 5 12 [Reporter]SCO2745 (10L20)_PCR/1611 [Gene]SCO2745/1476 NC_003888 SCC57A.16, probable LacI-family transcriptional regulator, len: 347 aa. Highly similar to many other regulators including: Escherichia coli SW:CYTR_ECOLI (EMBL:X03683) transcriptional repressor CytR (341 aa), fasta scores opt: 714 z-score: 751.4 E(): 0 34.9% identity in 332 aa overlap and Streptomyces coelicolor TR:Q9Z509 (EMBL:AL035591) putative transcriptional regulator SCC54.16 (368 aa), fasta scores opt: 843 z-score: 885.1 E():0 43.4% identity in 348 aa overlap. Contains a Prosite hit to PS00356 Bacterial regulatory proteins, lacI family signature and Pfam hits to PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family. The possible helix-turn-helix motif is situated between residues 2..23 (+5.89 SD). 4 1 5 11 [Reporter]SCO4070 (11H20)_PCR/1610 [Gene]SCO4070/1475 NC_003888 SCD25.06, unknown, len: 505 aa. 4 1 5 10 [Reporter]SCO4305 (12D20)_PCR/1609 [Gene]SCO4305/1474 NC_003888 SCD95A.38c, hypothetical protein, len: 197 aa; similar to TR:Q9X9V5 (EMBL:AL096743) Streptomyces coelicolor hypothetical 21.2 kD protein SCI7.35c, 197 aa; fasta scores: opt: 216 z-score: 272.7 E(): 9.9e-08; 35.2% identity in 199 aa overlap 4 1 5 9 [Reporter]SCO0253 (13P16)_PCR/1608 [Gene]SCO0253/1473 NC_003888 SCJ9A.32, probable tetR-family transcriptional regulator, len: 190 aa; similar to many e.g. SW:TER1_ECOLI TetR tetracycline resistance repressor from Escherichia coli (216 aa) fasta scores; opt: 501, z-score: 616.1, E(): 5.9e-27, (44.5% identity in 200 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and Prosite match to PS01081 Bacterial regulatory proteins, tetR family signature. Contains possible helix-turn-helix motif (+5.97 SD) 26-47 aa 4 1 5 8 [Reporter]SCO2773 (14L16)_PCR/1607 [Gene]SCO2773/1472 NC_003888 SCC105.04c, tesB2, acyl CoA thioesterase II, len: 290 aa; highly similar to SW:TESB_ECOLI (EMBL:M63308) Escherichia coli acyl CoA thioesterase II TesB, 285 aa; fasta scores: opt: 877 z-score: 1015.0 E(): 0; 48.6% identity in 286 aa overlap 4 1 5 7 [Reporter]SCO4975 (15H16)_PCR/1606 [Gene]SCO4975/1471 NC_003888 2SCK31.35, conserved hypothetical protein, len: 680 aa; similar to SW:YYAL_BACSU (EMBL:D26185) Bacillus subtilis hypothetical 78.8 kDa protein in TetB-ExoA intergenic region YyaL, 689 aa; fasta scores: opt: 1541 z-score: 1751.9 E(): 0; 39.2% identity in 683 aa overlap. Contains match to Prosite entry PS00133 Zinc carboxypeptidases, zinc-binding region 2 signature 4 1 5 6 [Reporter]SCO0071 (16D16)_PCR/1605 [Gene]SCO0071/1470 NC_003888 SCJ4.52c, unknown, len: 68 aa; predicted by GC Frameplot and amino acid usage. Note overlap with downstream gene. 1 4 16 22 [Reporter]SCO1202 (1M13)_PCR/6398 [Gene]SCO1202/5829 NC_003888 2SCG58.02, possible DNA ligase, len: 512 aa; similar to SW:DNLI_PYRFU Pyrococcus furiosus thermostable DNA ligase (EC 6.5.1.1) Lig, 561 aa; fasta scores: opt: 922 z-score: 971.8 E(): 0; 35.5% identity in 437 aa overlap. Contains Pfam match to entry PF01068 DNA_ligase, DNA ligase and match to Prosite entry PS00697 ATP-dependent DNA ligase AMP-binding site 1 4 16 21 [Reporter]SCO5523 (2I13)_PCR/6397 [Gene]SCO5523/5828 NC_003888 SC1C2.04, ilvE, probable branched-chain amino acid aminotransferase, len: 362 aa; similar to many, e.g. ILVE_BACSU putative branched-chain amino acid aminotransferase (362 aa), fasta scores; opt: 1047 z-score: 1401.5 E(): 0, 44.7% identity in 360 aa overlap. Contains PS00770 Aminotransferases class-IV signature 1 4 16 20 [Reporter]SCO2213 (3E13)_PCR/6396 [Gene]SCO2213/5827 NC_003888 SC10B7.08, probable regulatory protein, len: 193 aa; C-terminal region similar to C-terminal region of SW:GLNR_STRCO (EMBL:L03213) Streptomyces coelicolor transcriptional regulatory protein GlnR, 267 aa; fasta scores: opt: 330 z-score: 356.0 E(): 1.9e-12; 47.1% identity in 121 aa overlap. Contains Pfam match to entry PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 1 4 16 19 [Reporter]SCO5468 (4A13)_PCR/6395 [Gene]SCO5468/5826 NC_003888 SC2A11.02c, probable transmembrane transport protein, len: 472 aa; similar to many e.g. YHJE_ECOLI hypothetical metabolite transport protein (440 aa), fasta scores; opt: 772 z-score: 1638.5 E(): 0, 56.7% identity in 441 aa overlap and SHIA_ECOLI shikimate transporter (438 aa), fasta scores; opt: 553 z-score: 980.0 E(): 0, 34.6% identity in 437 aa overlap. Contains PS00216 Sugar transport proteins signature 1 and Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporters, score 183.10, E-value 4.4e-51 1 4 16 18 [Reporter]SCO2085 (5M9)_PCR/6394 [Gene]SCO2085/5825 NC_003888 SC4A10.18c, ftsW, probable cell division protein, len; 456 aa; previously sequenced as TR:Q9ZBA6 (EMBL:U10879), ftsW, Streptomyces coelicolor ftsW protein (456 aa) and identical to that sequence. Similar to many members of the ftsW/rodA/spoVE family e.g. SW:SP5E_BACSU (EMBL:X51419), spoVE, Bacillus subtilis stage V sporulation protein (366 aa), fasta scores; opt: 745 z-score: 788.4 E(): 0, 36.9% identity in 350 aa overlap. Shows similarity to SC6G9.31 (EMBL:AL079356) S.coelicolor probable integral membrane cell-cycle protein (446 aa) (30.0% identity in 416 aa overlap) and SCH69.16 (EMBL:AL079308) S.coelicolor probable FtsW/RodA/SpoVE family cell cycle protein (479 aa) (28.6% identity in 385 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF01098 FTSW_RODA_SPOVE, Cell cycle protein and PS00428 Cell cycle proteins ftsW/rodA/spoVE signature 1 4 16 17 [Reporter]SCO7454 (6I9)_PCR/6393 [Gene]SCO7454/5824 NC_003888 SC5C11.11, possible membrane protein, len: 264 aa. Contains possible membrane spanning hydrophobic domains. 1 4 16 16 [Reporter]SCO5680 (7E9)_PCR/6392 [Gene]SCO5680/5823 NC_003888 SC5H4.04c, probable cytidine deaminase, len: 137 aa; similar to TR:O53367 (EMBL:AL021841) Mycobacterium tuberculosis cytidine deaminase Cdd, 133 aa; fasta scores: opt 185 z-score: 244.6 E(): 3.6e-06; 37.4% identity in 115 aa overlap and to SW:CDD_MOUSE (EMBL:AA388666) Mus musculus cytidine deaminase (EC 3.5.4.5) Cdd, 146 aa; fasta scores: opt: 173 z-score: 229.1 E(): 2.6e-05; 29.2% identity in 113 aa overlap. Contains Pfam match to entry PF00383 dCMP_cyt_deam, Cytidine and deoxycytidylate deaminase zinc-binding region and match to Prosite entry PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature 1 4 16 15 [Reporter]SCO5322 (8A9)_PCR/6391 [Gene]SCO5322/5822 NC_003888 SC6G9.11c, conserved hypothetical protein, len: 289 aa; unknown function, similar to TR:O53410 (EMBL:AL021897) Mycobacterium tuberculosis hypothetical protein (285 aa), fasta scores; opt: 556 z-score: 639.1 E(): 2.9e-28, 38.8% identity in 286 aa overlap 4 2 23 5 [Reporter]SCO2992 (18H7)_PCR/4015 [Gene]SCO2992/3649 NC_003888 SCE50.20c, probable acetyltransferase, len: 164 aa; similar to TR:O66161 (EMBL:AB005901) Streptomyces kasugaensis kasugamycin acetyltransferase KAC338, 141 aa; fasta scores: opt: 756 z-score: 933.2 E(): 0; 76.8% identity in 142 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 4 16 14 [Reporter]SCO7826 (9M5)_PCR/6390 [Gene]SCO7826/5821 NC_003888 SC8E7.23c, unknown, len: 326 aa. 4 2 23 4 [Reporter]SCO6288 (19D7)_PCR/4014 [Gene]SCO6288/3648 NC_003888 SCBAC8D1.01, probable regulatory protein, len: 276 aa; similar to SW:AC24_STRCO (EMBL:M64683) Streptomyces coelicolor probable actinorhodin operon activatory protein actII-4, 255 aa; fasta scores: opt: 707 Z-score: 815.0 bits: 158.5 E(): 8.3e-38; 45.306% identity in 245 aa overlap,SC1G7.14, possible regulatory protein (fragment), len: >35 aa; similar to TR:Q54494 (EMBL:AJ224512) Streptomyces nogalater activator SnoA, 665 aa; fasta scores: opt: 86 Z-score: 144.4 bits: 32.8 E(): 1.9; 45.455% identity in 33 aa overlap 1 4 16 13 [Reporter]SCO2785 (10I5)_PCR/6389 [Gene]SCO2785/5820 NC_003888 SCC105.16, hypothetical protein, len: 595 aa; similar to SW:RHSF_RHIME (EMBL:AF110737) Rhizobium meliloti rhizobactin siderophore biosynthesis protein RhsF, 601 aa; fasta scores: opt: 1094 z-score: 1325.6 E(): 0; 32.4% identity in 592 aa overlap and to TR:P94255 (EMBL:U61153) Bordetella bronchiseptica AlcC, 618 aa; fasta scores: opt: 2037 z-score: 2470.2 E(): 0; 51.5% identity in 592 aa overlap 4 2 23 3 [Reporter]SCP1.174 (20P3)_PCR/4013 [Gene]SCP1.174/3647 NC_003888 SCP1.174, possible membrane protein, len: 147aa; similar to TR:Q9KY94 (EMBL:AL356813) putative septum site-determining protein from Streptomyces coelicolor (131 aa) fasta scores; opt: 129, z-score: 161.9, E(): 0.15, 33.3% identity in 141 aa overlap. Contains a possible membrane-spanning hydrophobic region. 4 2 22 22 [Reporter]SCO1095 (1H23)_PCR/4011 [Gene]SCO1095/3646 NC_003888 2SCG4.11, unknown, len: 338 aa. Contains highly hydrophilic C-terminal region 4 2 22 21 [Reporter]SCO2555 (2D23)_PCR/4010 [Gene]SCO2555/3645 NC_003888 SCC77.22c, hrcA, heat-inducible transcriptional repressor, len: 338 aa. Highly similar to many including: Bacillus stearothermophilus SW:HRCA_BACST (EMBL:Y09446) heat-inducible transcription repressor (344 aa), fasta scores opt: 610 z-score: 717.4 E(): 1.4e-32 29.5% identity in 332 aa overlap and Streptomyces albus TR:O52163(EMBL:AF025656) repressor of class I heat shock proteins, HrcA (338 aa), fasta scores opt: 1951 z-score: 2281.8 E():0 91.1% identity in 338 aa overlap. Contains a Pfam match to entry PF01628 HrcA. 4 2 22 20 [Reporter]SCO6434 (3P19)_PCR/4009 [Gene]SCO6434/3644 NC_003888 SC9B5.01, partial CDS, probable oxidoreductase, len: 353aa; overlaps with and forms the C-terminus of TR:O69828 on overlapping cosmid 1A6, similar to several eg. SW:PVDA_BURCE L-ornithine 5-monooxygenase from Burkholderia cepacia (444 aa) fasta scores; opt: 641, z-score: 580.6, E(): 4.7e-25,(40.5% identity in 316 aa overlap).,SC1A6.23, probable oxidoreductase, partial CDS, len: 133 aa; overlaps and forms the N-terminus of SC9B5.01 on overlapping cosmid 9B5, highly similar to an E. coli plasmid gene involved in siderophore biosynthesis IUCD_ECOLI P11295 Escherichia coli plasmid COLV-K30, lysine N-hydroxlyase (426 aa),fasta scores; opt: 298 z-score: 548.9 E(): 1.9e-23, 43.5% identity in 108 aa overlap 4 2 22 19 [Reporter]SCO7618 (4L19)_PCR/4008 [Gene]SCO7618/3643 NC_003888 SC2H2.16, probable transcriptional regulator, len: 264 aa; similar to SW:GYLR_STRGR (EMBL:M37327) Streptomyces griseus glycerol operon regulatory protein GylR, 254 aa; fasta scores: opt: 270 z-score: 322.5 E(): 2e-10; 34.0% identity in 253 aa overlap. Contains Pfam match to entry PF01614 IclR, Bacterial transcriptional regulator and possible helix-turn-helix motif at residues 42..63 (+3.28 SD) 4 1 5 5 [Reporter]SCO0865 (17P12)_PCR/1604 [Gene]SCO0865/1469 NC_003888 SCM2.18c, possible integral-membrane protein, len: 278 aa. Similar to Streptomyces coelicolor TR:CAB51458(EMBL:AL096884) putative membrane protein (236 aa), fasta scores opt: 184 z-score: 201.5 E(): 0.00077 31.3% identity in 265 aa overlap. Note this CDS appears to be a duplicated on this cosmid and shares 40.6 % identity, in 256 aa overlap, with the downstream gene, SCM2.20c. Contains a possible N-terminal signal sequence and possible membrane spanning hydrophobic domains. 4 2 22 18 [Reporter]SCO7105 (5H19)_PCR/4007 [Gene]SCO7105/3642 NC_003888 SC4B10.06c, possible RNA polymerase sigma factor, len: 301 aa; similar to TR:Q9S1V5 (EMBL:AL109950) Streptomyces coelicolor putative sigma factor SCJ24.18c, 250 aa; fasta scores: opt: 877 z-score: 1049.7 E(): 0; 54.7% identity in 236 aa overlap and to Streptomyces coelicolor St4B10.13c, 298; fasta scores: opt: 1112 z-score: 1131.2 E(): 0; 57.3% identity in 288 aa overlap. Also contains a possible helix-turn-helix motif at residues 133..154 (+3.38 SD) 4 1 5 4 [Reporter]SCO0692 (18L12)_PCR/1603 [Gene]SCO0692/1468 NC_003888 SCF42.02c, possible membrane protein, len: 485 aa. Rich in the amino acid Ala. Contains possible membrane spanning hydrophobic domains. 4 2 22 17 [Reporter]SCO7562 (6D19)_PCR/4006 [Gene]SCO7562/3641 NC_003888 SC5F1.16c, probable transcriptional regulator, len: 278 aa; similar to SW:ICLR_ECOLI (EMBL:M31761) Escherichia coli acetate operon repressor IclR, 274 aa; fasta scores: opt: 353 z-score: 395.0 E(): 1.8e-14; 30.0% identity in 243 aa overlap. Contains Pfam match to entry PF01614 IclR, Bacterial transcriptional regulator. Also contains possible helix-turn-helix motif at residues 36..57 (+6.31 SD) 4 1 5 3 [Reporter]SCP1.185 (19H12)_PCR/1602 [Gene]SCP1.185/1467 NC_003888 SCP1.185, conserved hypothetical protein, len: 184aa; similar to other hypothetical proteins from Streptomyces coelicolor eg. SCE6.05 (TR:Q9KZT3 EMBL:AL353832) (159 aa) fasta scores; opt: 518, z-score: 663.1, E(): 1.8e-29, 48.8% identity in 164 aa overlap and SCJ30.09C (TR:Q9S1Y0 EMBL:AL109973) (96 aa) fasta scores; opt: 438, z-score: 566.0, E(): 4.6e-24, 83.3% identity in 78 aa overlap. 4 2 22 16 [Reporter]SCO2109 (7P15)_PCR/4005 [Gene]SCO2109/3640 NC_003888 SC6E10.03, thiG, probable thiazole biosynthesis protein, len: 264 aa; similar to SW:THIG_ECOLI (EMBL:U00006), thiG, Escherichia coli thiamine biosynthetic enzyme (281 aa), fasta scores; opt: 750 z-score: 820.0 E(): 0, 49.2% identity in 244 aa overlap. Similar to many other thiG proteins. Highest similarity is to TR:P96263 (EMBL:Z84724) Mycobacterium tuberculosis hypothetical protein (252 aa), (71.7% identity in 251 aa overlap) 4 1 5 2 [Reporter]SCO1370 (20P8)_PCR/1601 [Gene]SCO1370/1466 NC_003888 SC10A9.12, probable two component system DNA binding response regulator, len: 211 aa; similar to SW:NARP_ECOLI (EMBL:L11273) Escherichia coli nitrate/nitrite response regulator protein NarP, 215 aa; fasta scores: opt: 467 z-score: 529.1 E(): 5e-22; 41.1% identity in 202 aa overlap and to TR:Q9RIM0 (EMBL:Y14336) Streptomyces reticuli response regulatory protein SenR, 217 aa; fasta scores: opt: 699 z-score: 785.2 E(): 0; 56.5% identity in 200 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Also contains possible helix-turn-helix motif at residues 164..185 (+4.15 SD) 4 1 4 22 [Reporter]SCO4202 (1P4)_PCR/1599 [Gene]SCO4202/1465 NC_003888 2SCD46.16c, possible NLP/P60-family secreted protein, len: 386aa; similar to many from Streptomyces coelicolor eg. TR:Q9XAE3 (EMBL:AL079356) putative secreted protein (391 aa) fasta scores; opt: 781, z-score: 663.2, E(): 1.9e-29, 38.9% identity in 378 aa overlap. Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family, a possible cleavable N-terminal signal sequence and coiled-coil regions at 75-118 and 196-233 residues. 4 1 4 21 [Reporter]SCO0765 (2L4)_PCR/1598 [Gene]SCO0765/1464 NC_003888 SCF81.24c, cel1, secreted endoglucanase, len: 747 aa; highly similar to SW:GUN1_STRRE (EMBL:X65616) Streptomyces reticulii cellulase 1 precursor (EC 3.2.1.4) (endoglucanase) (endo-1,4-beta-glucanase) (avicelase) Cel1, 746 aa; fasta scores: opt: 4213 z-score: 4621.9 E(): 0; 80.3% identity in 747 aa overlap. Contains two matches to Pfam entries PF00759 Glyco_hydro_9, Glycosyl hydrolase family 9 and to Prosite entry PS00698 Glycosyl hydrolases family 9 active sites signature 2. Also contains N-terminal region signal peptide sequence and a possible helix-turn-helix motif at residues 119..140 (+2.81 SD) 4 1 4 19 [Reporter]SCO6914 (4D4)_PCR/1596 [Gene]SCO6914/1463 NC_003888 SC1B2.20, unknown, len: 96 aa. 4 1 4 18 [Reporter]SCO7091 (4P24)_PCR/1595 [Gene]SCO7091/1462 NC_003888 SC3A4.17c, doubtful CDS, len: 48 aa. Contains a TTA/leucine codon, possible target for bldA regulation. 4 1 4 17 [Reporter]SCO7323 (5L24)_PCR/1594 [Gene]SCO7323/1461 NC_003888 SC4G10.02c, anti-sigma factor antagonist, len: 143 aa. Highly similar to many anti-anti sigma factors e.g. Bacillus subtilis SW:RSBS_BACSU (EMBL:L35574) anti-sigma factor antagonist, RsbS (121 aa), fasta scores opt: 341 z-score: 417.5 E(): 8.5e-16 43.4% identity in 113 aa overlap. Contains a Pfam match to entry PF01740 SpoIIAA, SpoIIAA family. 4 1 4 16 [Reporter]SCO6737 (6H24)_PCR/1593 [Gene]SCO6737/1460 NC_003888 SC5F2A.20, small proline-rich protein, len: 92 aa 1 4 16 12 [Reporter]SCO2279 (11E5)_PCR/6388 [Gene]SCO2279/5819 NC_003888 SCC75A.25c, unknown, len: 243 aa. 1 4 16 11 [Reporter]SCO4792 (12A5)_PCR/6387 [Gene]SCO4792/5818 NC_003888 SCD63A.03, probable two-component system DNA-binding response regulator, len: 251 aa; similar to SW:NARL_ECOLI (EMBL:X13360) Escherichia coli nitrate/nitrite response regulator protein NarL, 216 aa; fasta scores: opt: 428 z-score: 490.1 E(): 7.8e-20; 34.1% identity in 214 aa overlap and to TR:CAB51968 (EMBL:AL109661) Streptomyces coelicolor putative two-component system regulator SC6E10.14c, 215 aa; fasta scores: opt: 517 z-score: 472.3 E(): 9.4e-21; 44.3% identity in 219 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Contains also possible helix-turn-helix motif at residues 206..227 (+2.85 SD) 1 4 16 10 [Reporter]SCO3022 (13M1)_PCR/6386 [Gene]SCO3022/5817 NC_003888 SCE34.03c, unknown, len: 204 aa 1 4 16 9 [Reporter]SCO1630 (14I1)_PCR/6385 [Gene]SCO1630/5816 NC_003888 SCI41.13c, cvnA9, possible integral membrane protein, len: 541 aa. N-terminal domain similar to TR:CAB45635 (EMBL:AL079356) Streptomyces coelicolor SC6G9.44C possible histidine sensor kinase (806 aa); fasta scores: opt: 423 z-score: 422.2 E(): 3.8e-16; 26.4% identity in 481 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 16 8 [Reporter]SCO0173 (15E1)_PCR/6384 [Gene]SCO0173/5815 NC_003888 SCJ1.22c, unkown, len: 260 aa. 1 4 16 7 [Reporter]SCO0813 (16A1)_PCR/6383 [Gene]SCO0813/5814 NC_003888 SCF43A.03, probable oxidoreductase, len: 679 aa; similar to many members of the short-chain dehydrogenase/reductase family e.g. SW:YUXG_BACSU (EMBL:Z93938) Bacillus subtilis hypothetical oxidoreductase (689 aa), fasta scores; opt: 1727 z-score: 1840.8 E(): 0, 40.3% identity in 677 aa overlap. C-terminal half is similar to acetoin dehydrogenases e.g. SW:BUDC_KLEPN (EMBL:D86412), BudC, Klebsiella pneumoniae acetoin (diacetyl) reductase (256 aa) (35.7% identity in 258 aa overlap). C-terminal half is similar to putative dehydrogenases from S. coelicolor e.g. TR:O88068 (EMBL:AL031541) S.coelicolor probable dehydrogenase (260 aa) (34.3% identity in 268 aa overlap). Contains probable coiled-coil from 307 to 334 (28 residues) (Max score: 1.492, probability 0.94). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase 1 4 16 6 [Reporter]SCO1572 (16M21)_PCR/6382 [Gene]SCO1572/5813 NC_003888 SCL24.08, possible secreted protein, len: 238 aa; similar to TR:O07236 (EMBL:Z96800) Mycobacterium tuberculosis hypothetical 22.5 kD protein MTCY63.14, 218 aa; fasta scores: opt: 315 z-score: 359.1 E(): 1.4e-12; 31.2% identity in 218 aa overlap. Contains possible N-terminal region signal peptide sequence 1 4 16 5 [Reporter]SCO3818 (17I21)_PCR/6381 [Gene]SCO3818/5812 NC_003888 SCGD3.19, probable two-component system transcriptional regulator, len: 218 aa; similar to many two-component system regulators e.g. TR:O85372 (EMBL:AF064700), CprR, Rhodococcus sp. two-component system component controlling expression of an emulsion-stabilizing capsule (212 aa), fasta scores; opt: 807 z-score: 938.9 E(): 0, 60.7% identity in 211 aa overlap, but doesn't have a directly adjacent sensor kinase. Similar to many from S.coelicolor e.g. (EMBL:AL049754), SCH10.18c, probable two-component system regulator (228 aa) (42.2% identity in 211 aa overlap). Contains probable helix-turn-helix motif at aa 172-193 (Score 1200, +3.27 SD). Contains Pfam matches to entry PF00072 response_reg, Response regulator receiver domain and to entry PF00196 GerE, Bacterial regulatory proteins, luxR family. Contains PS00622 Bacterial regulatory proteins, luxR family signature 4 2 22 15 [Reporter]SCO2001 (8L15)_PCR/4004 [Gene]SCO2001/3639 NC_003888 SC7H2.15c, putative secreted protein, len: 597 aa; similar to TR:O86313 (EMBL:Z98260) hypothetical protein from Mycobacterium tuberculosis (411 aa) fasta scores; opt: 548, z-score: 447.1, E(): 1.4e-17, (33.7% identity in 380 aa overlap). Contains possible N-terminal region signal peptide sequence 1 4 16 4 [Reporter]SCO3169 (18E21)_PCR/6380 [Gene]SCO3169/5811 NC_003888 SCE87.20c, unknown, len: 75 aa. Note the predicted product of this CDS has an unusual amino acid composition with an excess of Arg, Asp and Gln residues. 4 2 22 14 [Reporter]SCO7538 (9H15)_PCR/4003 [Gene]SCO7538/3638 NC_003888 SC8G12.14, possible LysR-family transcriptional regulator, len: 322 aa. Similar to many regulators e.g. Escherichia coli SW:OXYR_ECOLI(EMBL:J04553) hydrogen peroxide-inducible genes activator, OxyR (305 aa), fasta scores opt: 323 z-score: 373.2 E(): 2.5e-13 29.4% identity in 296 aa overlap and Streptomyces coelicolor TR:CAB71819(EMBL:AL138662) putative LysR-family transcriptional regulator, SC8E4A.14C (300 aa), fasta scores opt: 488 z-score: 563.0 E(): 6.7e-24 34.0% identity in 288 aa overlap. Contains a Prosite hit to PS00044 Bacterial regulatory proteins, lysR family signature and a Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family with the putative helix-turn-helix motif situated between residues 24..45 (+4.98 SD). 1 4 16 3 [Reporter]SCO6303 (19A21)_PCR/6379 [Gene]SCO6303/5810 NC_003888 SCIF3.05c, conserved hypothetical protein, len: 323 aa; similar to TR:Q9X4X0 (EMBL:AF119621) Pseudomonas abietaniphila hypothetical protein which C-terminus is similar to isomerase/decarboxylase, DitH, 336 aa; fasta scores: opt: 481 z-score: 532.1 E(): 4.2e-22; 38.4% identity in 310 aa overlap. Contains Pfam match to entry PF01557 FAA_hydrolase, Fumarylacetoacetate (FAA) hydrolase family 4 2 22 13 [Reporter]SCO2433 (10D15)_PCR/4002 [Gene]SCO2433/3637 NC_003888 SCC24.04c, possible sugar transporter membrane protein, len: 313 aa; similar to TR: CAB59596(EMBL:AL132662) Streptomyces coelicolor probable sugar transporter inner membrane protein SCF11.19, 316 aa; fasta scores: opt: 533 z-score: 637.4 E(): 4.5e-28; 36.2% identity in 304 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 4 2 22 12 [Reporter]SCO4580 (11P11)_PCR/4001 [Gene]SCO4580/3636 NC_003888 SCD16A.03, probable fumarylacetoacetase, len: 418aa; similar to several from eukaryotes eg. SW:FAAA_HUMAN fumarylacetoacetase from human (419 aa) fasta scores; opt: 1192, z-score: 1370.0, E(): 0, (44.6% identity in 426 aa overlap). 4 2 22 11 [Reporter]SCO4158 (12L11)_PCR/4000 [Gene]SCO4158/3635 NC_003888 SCD84.25c, probable LacI-family regulatory protein, len: 340 aa; similar to TR:CAB66206 (EMBL:AL136502) Streptomyces coelicolor putative LacI-family transcriptional regulatory protein SCF43.17c, 341 aa; fasta scores: opt: 572 z-score: 669.2 E(): 8e-30; 37.3% identity in 338 aa overlap and to SW:CCPA_BACME (EMBL:L26052) Bacillus megaterium glucose-resistance amylase regulator (catabolite control protein) CcpA, 332 aa; fasta scores: opt: 564 z-score: 660.1 E(): 2.6e-29; 34.3% identity in 309 aa overlap. Contains Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and possible helix-turn-helix motif at residues 4..25 (+4.58 SD) 4 2 22 10 [Reporter]SCO3009 (13H11)_PCR/3999 [Gene]SCO3009/3634 NC_003888 SCE33.11c, hypothetical protein, len: 254 aa; similar to TR:O05886 (EMBL:Z95121) Mycobacterium tuberculosis hypothetical 24.5 kD protein MTCY20B11.16c, 214 aa; fasta scores: opt: 487 z-score: 551.0 E(): 3.1e-23; 47.6% identity in 210 aa overlap 4 2 22 9 [Reporter]SCO0686 (14D11)_PCR/3998 [Gene]SCO0686/3633 NC_003888 SCF15.07, possible membrane protein, 407 aa. Similar in parts to several hypothetical proteins e.g. Bacillus subtilis TR:O34437(EMBL:Z99108) hypothetical YfkH protein (275 aa), fasta scores: opt: 542 z-score: 589.5 E(): 1.8e-25 32.0% identity in 284 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 4 1 4 15 [Reporter]SCO1433 (7D24)_PCR/1592 [Gene]SCO1433/1459 NC_003888 SC6D7.06c, doubtful CDS, len: 154 aa. 4 2 22 8 [Reporter]SCO3556 (15P7)_PCR/3997 [Gene]SCO3556/3632 NC_003888 SCH5.19c, possible secretory protein, len: 523 aa; similar to many of undefined function eg. TR:O69627 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (352 aa) fasta scores; opt: 1287, z-score: 1191.3, E(): 0, (59.8% identity in 351 aa overlap) and SW:TRBB_AGRT6 conjugal transfer protein from Agrobacterium tumefaciens (323 aa) fasta scores; opt: 469, z-score: 441.4, E(): 2.8e-17, (31.4% identity in 287 aa overlap). Contains Pfam match to entry PF00437 GSPII_E, Bacterial type II secretion system protein, score 41.30, E-value 1.6e-11. Also contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00041 Bacterial regulatory proteins, araC family signature. 4 1 4 14 [Reporter]SCO7183 (8P20)_PCR/1591 [Gene]SCO7183/1458 NC_003888 SC8A11.11, probable branched amino acid transport system permease, len: 290 aa; similar to SW:LIVH_ECOLI (EMBL:J05516) Escherichia coli high-affinity branched-chain amino acid transport system permease LivH, 308 aa; fasta scores: opt: 427 z-score: 471.1 E(): 9e-19; 34.4% identity in 305 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 22 7 [Reporter]SCO1233 (16L7)_PCR/3996 [Gene]SCO1233/3631 NC_003888 2SCG1.08c, ureF, urease accessory protein, len: 224 aa; similar to SW:UREF_MYCTU (EMBL:U33011) Mycobacterium tuberculosis urease accessory protein UreF, 211 aa; fasta scores: opt: 401 z-score: 448.6 E(): 1.6e-17; 38.8% identity in 224 aa overlap. Contains Pfam match to entry PF01730 UreF, UreF and match to Prosite entry PS00099 Thiolases active site 4 1 4 13 [Reporter]SCO6455 (9L20)_PCR/1590 [Gene]SCO6455/1457 NC_003888 SC9B5.22, probable ABC transporter ATP-binding protein, len: 222aa; fifth of a five gene operon probably involved in peptide uptake, similar to many eg. SW:OPPF_SALTY oligopeptide transport ATP-binding protein, OppF, from Salmonella typhimurium (334 aa) fasta scores: opt: 412, z-score: 569.7, E(): 1.9e-24, (35.1% identity in 194 aa overlap). Contains PS00017 ATP /GTP-binding site motif A (P-loop). Also contains Pfam match to entry PF00005 ABC_tran, ABC transporters. 4 2 22 6 [Reporter]SCO1515 (17H7)_PCR/3995 [Gene]SCO1515/3630 NC_003888 SCL2.05c, secF, protein-export membrane protein, len: 373 aa; identical to previously sequenced SW:SECF_STRCO (EMBL:X85969) Streptomyces coelicolor protein-export membrane protein SecF, 373 aa and similar to SW:SECF_ECOLI (EMBL:X56175) Escherichia coli protein-export membrane protein SecF, 323 aa; fasta scores: opt: 488 z-score: 529.1 E(): 4.4e-22; 31.2% identity in 314 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 4 12 [Reporter]SCO2308 (10H20)_PCR/1589 [Gene]SCO2308/1456 NC_003888 SCC30.16, probable two-component system response regulator, len: 235 aa; similar to TR:Q9ZA47 (EMBL:AJ011500) Streptomyces violaceoruber putative two-component response regulator GRA-ORF10, 202 aa; fasta scores: opt: 711 z-score: 821.6 E(): 0; 55.0% identity in 200 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Contains also possible helix-turn-helix motif at residues 190..211 (+4.76 SD) 4 1 4 11 [Reporter]SCO4597 (11D20)_PCR/1588 [Gene]SCO4597/1455 NC_003888 SCD20.15c, possible two-component system sensor kinase, len: 455 aa; similar to TR:CAB81858 (EMBL:AL161691) Streptomyces coelicolor putative two-component sensor kinase SCD40A.13, 431 aa; fasta scores: opt: 754 z-score: 831.1 E(): 0; 40.2% identity in 433 aa overlap and to Streptomyces coelicolor putative two-component system sensor kinase SCD20.16c, 407 aa; fasta scores: opt: 1179 z-score: 1127.3 E(): 0; 57.5% identity in 442 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 4 10 [Reporter]SCO2906 (12P16)_PCR/1587 [Gene]SCO2906/1454 NC_003888 SCE19A.06, possible PTS transmembrane component, len: 431 aa; similar to many PTS (phosphoenolpyruvate-dependent sugar phosphotransferase system) transmembrane and sugar-binding components e.g. TR:Q57071 (EMBL:X93360), GlcA, PtsG, Staphylococcus carnosus PTS system, glucose-specific IIABC component (675 aa), fasta scores; opt: 1120 z-score: 1260.6 E(): 0, 43.4% identity in 431 aa overlap. Similar to the adjacent CDS SCE19A.07, possible PTS transmembrane component (416 aa) (4.1% identity in 429 aa overlap).The homologous PTS family proteins have a C-terminal extension not present in this CDS. Contains hydrophobic, possible membrane-spanning regions 4 1 4 9 [Reporter]SCO1177 (13L16)_PCR/1586 [Gene]SCO1177/1453 NC_003888 SCG11A.08, probable gntR-famly transcriptional regulator, len: 246 aa; similar to SW:DGOR_ECOLI (EMBL:L10328) Escherichia coli galactone operon transcriptional repressor GdoR, 229 aa; fasta scores: opt: 409 z-score: 460.9 E(): 2.7e-18; 36.5% identity in 219 aa overlap and to TR:O50536 (EMBL:AJ003022) Streptomyces coelicolor sporulation transcription factor WhiH, 295 aa; fasta scores: opt: 370 z-score: 416.5 E(): 8e-16; 35.2% identity in 230 aa overlap. Contians match to Pfam entry PF00392 gntR, Bacterial regulatory proteins, gntR family and match to Prosite entry PS00043 Bacterial regulatory proteins, gntR family signature 4 1 4 8 [Reporter]SCO3814 (14H16)_PCR/1585 [Gene]SCO3814/1452 NC_003888 SCGD3.15, possible DNA-binding protein, len: 325 aa; unknown function, probable CDS suggested by probable CDS suggested by positional base preference, GC frame analysis, amino acid composition and the presence of a possible RBS. Possible DNA-binding function as it contains a probable helix-turn-helix motif at aa 279-300 (Score 1121, +3.00 SD) 4 1 4 7 [Reporter]SCO3323 (15D16)_PCR/1584 [Gene]SCO3323/1451 NC_003888 SCE68.21, probable RNA polymerase sigma factor, len: 177 aa; similar to many members of the extracytoplasmic function sigma factor family e.g. SW:CARQ_MYXXA (EMBL:X71062), CarQ, Myxococcus xanthus RNA polymerase sigma factor (174 aa), fasta scores; opt: 237 z-score: 281.3 E(): 2.4e-08, 32.0% identity in 169 aa overlap. Similar to others from S. coelicolor e.g. TR:O54163 (EMBL:AL021411), SC7H1.04, possible sigma factor (200 aa) (30.8% identity in 172 aa overlap). Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 4 1 4 6 [Reporter]SCO1836 (16P12)_PCR/1583 [Gene]SCO1836/1450 NC_003888 SCI8.21c, possible stress-like protein, len: 160 aa. Similar to several stress proteins e.g. Staphylococcus aureus TR:Q53485 (EMBL: S76213) alkaline shock protein 23 (169 aa), fasta scores opt: 303 z-score: 340.6 E(): 1.3e-11 39.3% identity in 150 aa overlap and Enterococcus faecalis TR:O32593 (EMBL: AJ000042) Gls24 general stress protein (180 aa), fasta scores opt: 218 z-score: 249.4 E(): 1.6e-06 31.7% identity in 126 aa overlap. 1 4 16 2 [Reporter]SCO5986 (20I17)_PCR/6378 [Gene]SCO5986/5809 NC_003888 SCBAC16H6.21, possible oxidoreductase, len: 275aa: similar to many eg. TR:O53622 (EMBL:AL021428) putative oxidoreductase from Mycobacterium tuberculosis (276 aa) fasta scores; opt: 521, Z-score: 601.4, 36.364% identity (38.314% ungapped) in 275 aa overlap and TR:Q53042 (EMBL:AF060871) haloalkane dehalogenase from Rhodococcus rhodochrous (293 aa) fasta scores; opt: 249, Z-score: 291.2, 28.417% identity (31.102% ungapped) in 278 aa overlap. 1 4 15 22 [Reporter]SCO1104 (1I13)_PCR/6376 [Gene]SCO1104/5808 NC_003888 2SCG4.20, probable tetR-family transcriptional regulatory protein, len: 233 aa; similar to SW:BETI_ECOLI (EMBL:X52905) Escherichia coli regulatory protein BetI, 195 aa; fasta scores: opt: 177 z-score: 211.3 E(): 0.00027; 24.5% identity in 196 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Also contains possible helix-turn-helix at residues 36..57 (+3.64 SD) 1 4 15 21 [Reporter]SCO2969 (2E13)_PCR/6375 [Gene]SCO2969/5807 NC_003888 SCE59.28c, ftsE, cell division ATP-binding protein, len: 229 aa; similar to TR:O05779 (EMBL:Z95150) Mycobacterium tuberculosis FtsE, 229 aa; fasta scores: opt: 908 z-score: 1050.8 E(): 0; 62.4% identity in 226 aa overlap and to SW:FTSE_ECOLI (EMBL:X04398) Escherichia coli cell division ATP-binding protein FtsE, 222 aa; fasta scores: opt: 646 z-score: 751.1 E(): 0; 49.1% identity in 216 aa overlap . Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, two matches to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and match to Prosite entry PS00211 ABC transporters family signature 1 4 15 20 [Reporter]SCO2207 (3A13)_PCR/6374 [Gene]SCO2207/5806 NC_003888 SC10B7.02, hypothetical secreted protein, len: 231 aa; identical to previously sequenced TR:Q9X955 (EMBL:Y13833) Streptomyces coelicolor hypothetical 23.4 kD protein precursor. Contains a possible N-terminal region signal peptide sequence 1 4 15 18 [Reporter]SCO2081 (5I9)_PCR/6372 [Gene]SCO2081/5805 NC_003888 SC4A10.14c, conserved hypothetical protein, len: 242 aa; unknown function, similar to many hypothetical proteins e.g. SW:YFIH_STRGR (EMBL:U07344) Streptomyces griseus hypothetical protein (246 aa), fasta scores; opt: 1246 z-score: 1349.0 E(): 0 76.3% identity in 245 aa overlap and SW:YFIH_ECOLI (EMBL:U50134), YfiH, Escherichia coli hypothetical protein (243 aa) (38.5% identity in 247 aa overlap) 1 4 15 17 [Reporter]SCO5780 (6E9)_PCR/6371 [Gene]SCO5780/5804 NC_003888 SC4H2.01c, probable secreted protein, len: 331 aa; some similarity to a secreted protein from Brucella abortus BCSP_BRUAB P12920 31 kd immunogenic protein precursor (329 aa), fasta scores; opt: 399 z-score: 357.4 E(): 1.1e-12, 32.7% identity in 336 aa overlap. Contains probable N-terminal signal sequence 1 4 15 16 [Reporter]SCO7252 (7A9)_PCR/6370 [Gene]SCO7252/5803 NC_003888 SC5H1.40c, possible regulatory protein, len: 502 aa; similar to e.g. SW:SP15_STRGR (EMBL:M32687) Streptomyces griseus sporulation protein (529 aa), fasta scores; opt: 394 z-score: 452.2 E(): 7.2e-18, 27.9% identity in 530 aa. Similar to SC7A1.26 (EMBL:AL034447) S.coelicolor possible transcriptional regulator (500 aa) (43.0% identity in 491 aa overlap) 1 4 15 15 [Reporter]SCO7246 (8M5)_PCR/6369 [Gene]SCO7246/5802 NC_003888 SC7A12.13, unknown, len: 80 aa. Highly similar to Streptomyces coelicolor TR:Q53896 (EMBL:X60316) pleiotropic antibiotic regulatory locus AbaA, OrfD (75 aa), fasta scores opt: 195 z-score: 288.2 E(): 1.4e-08 45.8% identity in 72 aa overlap. 4 2 22 4 [Reporter]SCO6106 (19P3)_PCR/3993 [Gene]SCO6106/3629 NC_003888 SCBAC1A6.30c, possible acyl-CoA dehydrogenase, len: 557 aa; similar to SW:AIDB_ECOLI (EMBL:L20915) Escherichia coli AidB protein, 541 aa; fasta scores: opt: 916 z-score: 1055.7 E(): 0; 45.5% identity in 539 aa overlap and to TR:CAC13064 (EMBL:AL445503) Streptomyces coelicolor putative oxidoreductase 2SCG38.05, 567 aa; fasta scores: opt: 1480 z-score: 1414.3 E(): 0; 61.8% identity in 555 aa overlap. Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase and match to Prosite entry PS00072 Acyl-CoA dehydrogenases signature 1 and PS00073 Acyl-CoA dehydrogenases signature 2 1 4 15 14 [Reporter]SCO7822 (9I5)_PCR/6368 [Gene]SCO7822/5801 NC_003888 SC8E7.19c, probable prolipoprotein diacylglyceryl transferase, len: 26c. Highly similar to many including: Bacillus subtilis SW:LGT_BACSU(EMBL:AF017113) prolipoprotein diacylglyceryl transferase (EC 2.4.99.-) (269 aa), fasta scores opt: 469 z-score: 520.6 E(): 1.6e-21 32.7% identity in 251 aa overlap and Streptomyces coelicolor TR:Q9S2U8(EMBL:AL096884) putative prolipoprotein diacyleglyceryl transferase, SC4G6.03C (343 aa), fasta scores opt: 1330 z-score: 1457.8 E():0 65.1% identity in 292 aa overlap. Contains a Pfam match to entry PF01790 LGT, Prolipoprotein diacylglyceryl transferase and multiple possible membrane spanning hydrophobic domains. 4 2 22 3 [Reporter]SCO4901 (20L3)_PCR/3992 [Gene]SCO4901/3628 NC_003888 2SCK8.27, probable adenosine deaminase, len: 396 aa; similar to SW:ADD_ECOLI (EMBL:M59033) Escherichia coli adenosine deaminase (EC 3.5.4.4) Add, 333 aa; fasta scores: opt: 421 z-score: 505.2 E(): 1.4e-20; 32.2% identity in 338 aa overlap and to TR:Q9X7T2 (EMBL:AL049863) Streptomyces coelicolor putative adenosine deaminase SC5H1.24c, 359 aa; fasta scores: opt: 1373 z-score: 1637.4 E(): 0; 60.7% identity in 351 aa overlap. Contains Pfam match to entry PF00962 A_deaminase, Adenosine/AMP deaminase 1 4 15 13 [Reporter]SCO5134 (10E5)_PCR/6367 [Gene]SCO5134/5800 NC_003888 SC9E12.19c, hypothetical protein, len: 344 aa; highly similar to TR:O87087 (EMBL:AB006206) Streptomyces griseus AmfR, AmfA and AmfB genes and 4 ORFS, complete CDS, ORF5, 264 aa; fasta scores: opt: 1018 z-score: 1086.7 E(): 0; 63.3% identity in 264 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 4 2 21 22 [Reporter]SCO1091 (1D23)_PCR/3989 [Gene]SCO1091/3627 NC_003888 2SCG4.07c, possible acetyltransferase, len: 179 aa; similar to TR:Q9ZBJ7 (EMBL:AL035161) Streptomyces coelicolor hypothetical 19.8 kD protein SC9C7.13c, 179 aa; fasta scores: opt: 131 z-score: 171.3 E(): 0.047; 29.3% identity in 157 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 4 2 21 21 [Reporter]SCO2609 (2P19)_PCR/3988 [Gene]SCO2609/3626 NC_003888 SCC88.20c, mreD, rod shape-determining protein, len: 223 aa; identical to previously sequenced TR:O33621 (EMBL:Y14206) Streptomyces coelicolor MreD protein, 223 aa and similar to TR:O83512 (EMBL:AE001226) Treponema pallidum rod shape-determining protein (MreD), 168 aa; fasta scores: opt: 135 z-score: 162.4 E(): 0.13; 24.8% identity in 161 aa overlap. Contains possible N-terminal region signal peptide sequence 4 2 21 20 [Reporter]SCO6430 (3L19)_PCR/3987 [Gene]SCO6430/3625 NC_003888 SC1A6.19, possible integral membrane protein, len: 630 aa; N-terminus (start to aa ~400) is hydrophobic, C-terminus is proline-rich 4 2 21 19 [Reporter]SCO5618 (4H19)_PCR/3986 [Gene]SCO5618/3624 NC_003888 SC2E1.35, unknown prophage gene, len: 147 aa 4 2 21 18 [Reporter]SCO7101 (5D19)_PCR/3985 [Gene]SCO7101/3623 NC_003888 SC4B10.02, probable dehydrogenase, len: 451 aa; similar to SW:DHNA_ECOLI (EMBL:V00306) Escherichia coli NADH dehydrogenase (EC 1.6.99.3) Ndh, 433 aa; fasta scores: opt: 472 z-score: 552.4 E(): 2.7e-23; 31.6% identity in 377 aa overlap 4 1 4 5 [Reporter]SCO0388 (17L12)_PCR/1582 [Gene]SCO0388/1449 NC_003888 SCF62.14, unknown, len: 653 aa. Weakly similar to Cytophaga heparina TR: Q59289 (EMBL; U27586) heparinase III protein precursor (EC 4.2.2.8) (659 aa), fasta scores opt: 202 z-score: 229.2 E(): 2.1e-05 22.8% identity in 421 aa overlap. 4 2 21 17 [Reporter]SCO2184 (6P15)_PCR/3984 [Gene]SCO2184/3622 NC_003888 SC5F7.17, possible transcriptional regulator, len: 327aa; similar to TR:O34920 (EMBL:AF027868) putative transcriptional regulator from Bacillus subtilis (313 aa) fasta scores; opt: 319, z-score: 380.6, E(): 7.3e-14, (27.3% identity in 330 aa overlap). Contains helix-turn-helix motif (+4.15 SD) 20-41aa. 4 1 4 4 [Reporter]SCO2948 (18H12)_PCR/1581 [Gene]SCO2948/1448 NC_003888 SCE59.07c, hypothetical protein, len: 193 aa; similar to various hypothetical proteins, e.g. TR:P71608 (EMBL:Z80233) Mycobacterium tuberculosis hypothetical 21.8 kD protein CY10H4.38, 202 aa; fasta scores: opt: 525 z-score: 594.5 E(): 1.1e-25; 46.7% identity in 182 aa overlap 4 2 21 16 [Reporter]SCO1407 (7L15)_PCR/3983 [Gene]SCO1407/3621 NC_003888 SC6D7.32, unknown, len: > 1425 aa. Weakly similar to several including: Streptomyces coelicolor TR:Q9Z5A4(EMBL:AL035478) putative secreted protein SC2G5.19 (1156 aa), fasta scores opt: 327 z-score: 314.0 E(): 4.1e-10 28.0% identity in 938 aa overlap and Rhodobacter capsulatus TR:O68032(EMBL:AF010496) potential exonuclease (1238 aa), fasta scores opt: 439 z-score: 421.6 E(): 4.1e-16 28.8% identity in 1154 aa overlap. The predicted protein product of this CDS is alanine rich and contains multiple probable coiled-coils between residues: 369 to 401 (33 residues), 406 to 433 (28 residues), 483 to 519 (37 residues), 563 to 604 (42 residues),850 to 921 (72 residues),926 to 982 (57 residues), 1025 to 1054 (30 residues),1072 to 1106 (35 residues), 1124 to 1167 (44 residues), 1199 to 1232 (34 residues) and 1278 to 1309 (32 residues).,SC1A8A.26c, partial CDS, unknown, len: >162aa; forms the C-terminal region of SC6D7.32 (TR:CAB61684 EMBL:AL133213) which has weak similarity to proteins of unknown function. 4 1 4 3 [Reporter]SCP1.80 (19D12)_PCR/1580 [Gene]SCP1.80/1447 NC_003888 SCP1.80, unknown, len: 333aa; full length similarity with TR:Q54402 (EMBL:Z11519) hypothetical protein from transposable element, Tn4811 of Streptomyces lividans 66 (336 aa) fasta scores; opt: 1080, z-score: 1214.2, E(): 0, 49.0% identity in 335 aa overlap. C-terminal similar to SCP1.40c/SCP1.314 (previously sequenced as TR:Q9L8H3 (EMBL:AF204735)) (87 aa) fasta scores; opt: 311, z-score: 364.2, E(): 8e-13, 50.0% identity in 82 aa overlap. 4 2 21 15 [Reporter]SCO5234 (8H15)_PCR/3982 [Gene]SCO5234/3620 NC_003888 SC7E4.31, possible sugar transporter integral membrane protein, len: 276 aa; similar to TR:Q9RK77 (EMBL:AL132662;) Streptomyces coelicolor putative sugar transporter integral membrane protein SCF11.13, 285 aa; fasta scores: opt: 1027 z-score: 1227.9 E(): 0; 54.3% identity in 278 aa overlap and to TR:Q9R9Q5 (EMBL:AF175299) Rhizobium meliloti putative inner membrane permease ThuG, 276 aa; fasta scores: opt: 554 z-score: 666.6 E(): 1.2e-29; 33.8% identity in 266 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 4 1 3 22 [Reporter]SCO4198 (1L4)_PCR/1577 [Gene]SCO4198/1446 NC_003888 2SCD46.12, probable DNA-binding protein, len: 141 aa; N-terminal region similar to many eg. TR:O53759 (EMBL:AL021933) putative regulatory protein from Mycobacterium tuberculosis (140 aa) fasta scores; opt: 412, z-score: 503.0, E(): 1.6e-20, 56.6% identity in 113 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and two helix-turn-helix motifs; (Score 979 (+2.52 SD)) at residue 51-72 and (Score 1963 (+5.87 SD)) at residue 22-43. 4 1 3 21 [Reporter]SCO0777 (2H4)_PCR/1576 [Gene]SCO0777/1445 NC_003888 3SCF60.09, conserved hypothetical protein, len: 341 aa; identical to TR:O85700 (EMBL:AF072709) Streptomyces lividans hypothetical 38.1 kDa protein, 341 aa and similar to TR:AAG05998 (EMBL:AE004689) Pseudomonas aeruginosa conserved hypothetical protein PA2610, 324 aa; fasta scores: opt: 838 z-score: 962.6 E(): 0; 44.6% identity in 323 aa overlap. Contains Pfam match to entry PF00043 GST, Glutathione S-transferases 4 1 3 20 [Reporter]SCO7337 (3D4)_PCR/1575 [Gene]SCO7337/1444 NC_003888 SC4G10.16, pepA2, unknown, len: 171 aa. Previously sequenced region: Streptomyces coelicolor TR:O54205(EMBL:AJ001206) hypothetical protein, PepA2 (171 aa). 4 1 3 19 [Reporter]SCO6153 (3P24)_PCR/1574 [Gene]SCO6153/1443 NC_003888 SC1A9.17c, possible regulatory protein, len: 424 aa; similar to TR:Q56084 (EMBL:D63904) ESA36 protein of undefined function from Streptomyces thermoviolaceus (334 aa) fasta scores; opt: 313, z-score: 401.9, E(): 4.1e-15, (30.0% identity in 337 aa overlap). Also similar to SW:BRPA_STRHY bialaphos biosynthetic pathway regulatory protein from Streptomyces hygroscopicus (256 aa), fasta scores; opt: 234, z-score: 233.8, E(): 9.5e-06, (30.1% identity in 163 aa overlap). Contains helix-turn-helix motif from: 1 to: 424, Score 1064 (+2.81 SD). Possible coiled-coil from 171 to 200 (30 residues) Max score: 1.447 (probability 0.87) 4 1 3 18 [Reporter]SCO7087 (4L24)_PCR/1573 [Gene]SCO7087/1442 NC_003888 SC3A4.13c, possible oxidoreductase, len: 312 aa. Similar to many including: Streptomyces coelicolor TR:CAB61562(EMBL:AL133171) putative aldo/keto reductase (fragment), SCF81.28C (307 aa), fasta scores opt: 351 z-score: 407.7 E():3e-15 30.7% identity in 309 aa overlap. 4 1 3 17 [Reporter]SCO6563 (5H24)_PCR/1572 [Gene]SCO6563/1441 NC_003888 SC4B5.13, probable integral membrane transporter, len: 446aa; similar to several eg. TR:Q51330 (EMBL:U40075) OxlT, the oxalate:formate exchange protein from Oxalobacter formigenes (418 aa), fasta scores; opt: 243, z-score: 382.3, E(): 5.3e-14, (26.1% identity in 421 aa overlap). Contains several possible membrane spanning regions. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter, score -99.90, E-value 0.013. 4 1 3 16 [Reporter]SCO6733 (6D24)_PCR/1571 [Gene]SCO6733/1440 NC_003888 SC5F2A.16c, possible transcriptional regulator, len: 251aa; similar to many eg. SW:SOXR_PSEAE SoxR regulator of the superoxide response regulon (156 aa) fasta scores; opt: 181, z-score: 219.0, E(): 6.9e-05, (37.1% identity in 105 aa overlap). Contains probable helix-turn-helix motif (+3.41 SD) 28-49aa. 4 2 21 14 [Reporter]SCO7534 (9D15)_PCR/3981 [Gene]SCO7534/3619 NC_003888 SC8G12.10, possible two component system histidine kinase, len: 504 aa. Highly similar to several e.g. Streptomyces coelicolor SW:AFQ2_STRCO(EMBL:D10654) sensor protein AfsQ2 (EC 2.7.3.-) (535 aa), fasta scores opt: 1060 z-score: 1143.7 E(): 0 41.3% identity in 506 aa overlap. Contains Pfam matches to entries PF00672 DUF5, Domain found in bacterial signal proteins and PF00512 signal, Histidine kinase domain, as well as a possible N-terminal hydrophobic membrane spanning region 4 2 21 13 [Reporter]SCO2583 (10P11)_PCR/3980 [Gene]SCO2583/3618 NC_003888 SCC123.21c, possible membrane protein, len: 356 aa. similar to TR:Q9L0J6 (EMBL:AL161691) SCD40A.15 putative membrane protein from Streptomyces coelicolor (379 aa) fasta scores; opt: 350, Z-score: 385.8, E(): 7.4e-14, 30.056% identity (33.025% ungapped) in 356 aa overlap. Contains a possible membrane spanning hydrophobic domain. 4 2 21 12 [Reporter]SCO4340 (11L11)_PCR/3979 [Gene]SCO4340/3617 NC_003888 SCD12A.23, possible integrase, len: 549 aa; similar to TR:CAB75384 (EMBL:AL139298) Streptomyces coelicolor putative integrase SCC88.14, 518 aa; fasta scores: opt: 232 z-score: 262.7 E(): 3.6e-07; 26.5% identity in 548 aa overlap 4 2 21 11 [Reporter]SCO4180 (12H11)_PCR/3978 [Gene]SCO4180/3616 NC_003888 SCD66.17, probable iron uptake regulatory protein, len: 145 aa; similar to SW:FUR_CAMJE (EMBL:X78965) Campylobacter jejuni ferric uptake regulation protein Fur, 157 aa; fasta scores: opt: 274 z-score: 366.6 E(): 6e-13; 31.9% identity in 135 aa overlap. Contains Pfam match to entry PF01475 FUR, Ferric uptake regulator family 4 2 21 10 [Reporter]SCO3004 (13D11)_PCR/3977 [Gene]SCO3004/3615 NC_003888 SCE33.06, unknown, len: 89 aa. High content in arginine amino acid residues and also high % G+C (77.4%) 4 2 21 9 [Reporter]SCO0937 (14P7)_PCR/3976 [Gene]SCO0937/3614 NC_003888 SCM10.25, hypothetical protein, len: 153 aa; similar to various hypothetical proteins, e.g. TR:BAA86264 (EMBL:AB023785) Streptomyces griseus ORF2, 173 aa; fasta scores: opt: 218 z-score: 266.9 E(): 1.8e-07; 35.5% identity in 138 aa overlap 4 2 21 8 [Reporter]SCO5036 (15L7)_PCR/3975 [Gene]SCO5036/3613 NC_003888 SCK7.09c, hypothetical protein, len: 174 aa; similar to TR:O50432 (EMBL:AL010186) Mycobacterium tuberculosis hypothetical 21.1 kDa protein MTV005.12c, 189 aa; fasta scores: opt: 183 z-score: 238.8 E(): 7.8e-06; 30.1% identity in 186 aa overlap 4 1 3 15 [Reporter]SCO6951 (7P20)_PCR/1570 [Gene]SCO6951/1439 NC_003888 SC6F7.04, unknown, len: 293 aa. Similar to Streptomyces coelicolor TR:Q9ZEP2(EMBL:AJ131213) hypothetical 30.7 kd protein, SCE94.11C (277 aa), fasta scores: opt: 214 z-score: 251.4 E(): 1.5e-06 26.6% identity in 286 aa overlap 4 2 21 7 [Reporter]SCO0212 (16H7)_PCR/3974 [Gene]SCO0212/3612 NC_003888 SCJ12.24c, unknown, len: 157 aa. 4 1 3 14 [Reporter]SCO7179 (8L20)_PCR/1569 [Gene]SCO7179/1438 NC_003888 SC8A11.07c, possible secreted amidase, len: 642 aa; similar to TR:Q9S2M7 (EMBL:AL109661) Streptomyces coelicolor hypothetical 100.7 kDa protein SC6E10.10, 943 aa; fasta scores: opt: 1082 z-score: 1143.6 E(): 0; 40.2% identity in 684 aa overlap and to SW:CWHA_ACHLY Achromobacter lyticus N-acetylmuramoyl-L-alanine amidase A (EC 3.5.1.28) CwhA, 177 aa; fasta scores: opt: 214 z-score: 235.8 E(): 1.1e-05; 29.4% identity in 160 aa overlap. Contains Pfam match to entry PF01510 Amidase_2, N-acetylmuramoyl-L-alanine amidase. Also contains possible N-terminal region signal peptide sequence 4 2 21 6 [Reporter]SCO0249 (17D7)_PCR/3973 [Gene]SCO0249/3611 NC_003888 SCJ9A.28c, possible monooxygenase, len: 504 aa; similar to many e.g. TR:Q9ZGM8 (EMBL:AF077869) oxygenase involved in the last step of mithramycin biosynthesis in Streptomyces argillaceus (533 aa) fasta scores; opt: 1215, z-score: 1311.5, E(): 0, (45.0% identity in 513 aa overlap). Contains Pfam matches to entry PF01360 Monooxygenase, Monooxygenase and Pfam match to entry PF01494 FAD_binding_3, and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible uncleavable N-terminal signal sequence. 4 1 3 13 [Reporter]SCO7166 (9H20)_PCR/1568 [Gene]SCO7166/1437 NC_003888 SC9A4.28c, probable sugar-transporter integral membrane protein, len: 310 aa; similar to TR:Q9RJV7 (EMBL:AL117387) Streptomyces coelicolor putative binding protein dependent transport protein SCF41.12, 303 aa; fasta scores: opt: 913 z-score: 1073.6 E(): 0; 46.9% identity in 309 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 4 2 21 5 [Reporter]SCO3354 (18P3)_PCR/3972 [Gene]SCO3354/3610 NC_003888 SCE94.05, unknown, proline-rich, len: aa; 4 1 3 12 [Reporter]SCO2304 (10D20)_PCR/1567 [Gene]SCO2304/1436 NC_003888 SCC30.12c, possible dehydrogenase, len: 333 aa; similar to SW:DHB4_HUMAN (EMBL:X87176) Homo sapiens, estradiol 17 beta-dehydrogenase 4 (EC 1.1.1.62) Hsd17b4, 736 aa; fasta scores: opt: 611 z-score: 633.2 E(): 8e-28; 34.7% identity in 288 aa overlap. Contains Pfam match to entry PF01575 MaoC_like, MaoC like domain 4 1 3 11 [Reporter]SCO4520 (11P16)_PCR/1566 [Gene]SCO4520/1435 NC_003888 SCD35.27c, hypothetical protein, len: 101 aa; similar to TR:Q9KYA2 (EMBL:AL356813) Streptomyces coelicolor conserved hypothetical protein SCK15.02, 102 aa; fasta scores: opt: 471 Z-score: 568.2 E(): 5.2e-24; 73.684% identity in 95 aa overlap 4 1 3 10 [Reporter]SCO2902 (12L16)_PCR/1565 [Gene]SCO2902/1434 NC_003888 SCE19A.02c, conserved hypothetical protein, len: 200 aa; unknown function, similar to many e.g. SW:Y03Q_MYCTU (EMBL:Z73902) Mycobacterium tuberculosis hypothetical protein (204 aa), fasta scores; opt: 633 z-score: 743.6 E(): 0, 52.0% identity in 200 aa overlap. Similar to SW:YGGV_ECOLI (EMBL:U28377), yggV, Escherichia coli hypothetical protein (197 aa) (49.5% identity in 198 aa overlap) 4 1 3 9 [Reporter]SCO0289 (13H16)_PCR/1564 [Gene]SCO0289/1433 NC_003888 SCF85.17c, probable transcriptional regulator, len: 342 aa. Similar to many e.g. Streptomyces lividans TR:Q9ZI18 (EMBL; AF043654) BxlR (338 aa), fasta scores opt: 753 z-score: 849.8 E(): 0 42.9% identity in 333 aa overlap and Streptomyces coelicolor TR:O86795 (EMBL; AL031317) putative transcriptional regulator SC6G4.32 (355 aa), fasta scores opt: 880 z-score: 991.6 E(): 0 47.5% identity in 339 aa overlap. Contains Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family, Pfam match to entry PF00356 lacI, Bacterial regulatory proteins, lacI family and a PS00356 Bacterial regulatory proteins, lacI family signature. Has a helix turn helix motif between aa 9..30 4 1 3 8 [Reporter]SCO1069 (14D16)_PCR/1563 [Gene]SCO1069/1432 NC_003888 SCG22.15c, possible marR-family transcriptional regulator, len: 159 aa; similar to TR:Q9X8V0 (EMBL:AL049826) Streptomyces coelicolor putative transcriptional regulator SCH24.36c, 154 aa; fasta scores: opt: 302 z-score: 391.5 E(): 2.5e-14; 41.4% identity in 128 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family and match to Prosite entry PS01117 Bacterial regulatory proteins, marR family signature. Contains also a possible helix-turn-helix motif at residues 89..110 (+4.56 SD) 4 1 3 7 [Reporter]SCO3480 (15P12)_PCR/1562 [Gene]SCO3480/1431 NC_003888 SCE65.16c, possible racemase, len: 361 aa; similar to TR:CAB55709 (EMBL:AL117387) Streptomyces coelicolor possible racemase SCF41.06, 416 aa; fasta scores: opt: 471 z-score: 557.6 E(): 1.1e-23; 32.6% identity in 328 aa overlap and to SW:MANR_PSEPU (EMBL:M19043) Pseudomonas putida mandelate racemase (EC 5.1.2.2) MdlA, 359 aa; fasta scores: opt: 473 z-score: 561.0 E(): 7.2e-24; 27.8% identity in 363 aa overlap. Contains Pfam match to entry PF01188 MR_MLE, Mandelate racemase / muconate lactonizing enzyme family and match to Prosite entry PS00909 Mandelate racemase / muconate lactonizing enzyme family signature 2 4 1 3 6 [Reporter]SCO0768 (16L12)_PCR/1561 [Gene]SCO0768/1430 NC_003888 SCF81.27, possible lipoprotein, len: 221 aa. Contains N-terminal signal peptide sequence, and appropriately positioned Prosite entry PS00013 probable lipoprotein Prokaryotic membrane lipoprotein lipid attachment sit 1 1 1 12 [Reporter]SCO2342 (10I16)_PCR/11 [Gene]SCO2342/9 NC_003888 SCC53.33c, possible secreted protein, len: 223aa; contains a possible non-cleavable N-terminal signal sequence. 1 1 1 11 [Reporter]SCO4348 (11E16)_PCR/10 [Gene]SCO4348/8 NC_003888 SCD19.03c, hypothetical protein, len: 723 aa; similar to some plasmid derived hypothetical proteins, e.g. TR:O85873 (EMBL:AF079317) Sphingomonas aromaticivorans hypothetical 81.3 kDa protein (plasmid pNL1), 741 aa; fasta scores: opt: 421 z-score: 478.1 E(): 4e-19; 29.7% identity in 758 aa overlap 1 1 1 10 [Reporter]SCO4256 (12A16)_PCR/9 [Gene]SCO4256/7 NC_003888 SCD8A.29, possible hydrolytic protein, len: 479 aa; similar to TR:Q9ZBF7 (EMBL:AL035206) Streptomyces coelicolor putative hydrolytic protein SC9B5.23c, 464 aa; fasta scores: opt: 1820 z-score: 1645.7 E(): 0; 70.9% identity in 423 aa overlap. Contains possible hydrophobic membrane spanning region 1 1 1 9 [Reporter]SCO3455 (13M12)_PCR/8 [Gene]SCO3455/6 NC_003888 SCE46.12c, possible ABC-transporter transport protein, len: 285 aa; similar to TR:O57756 (EMBL:AP000001) Pyrococcus horikoshii 284 aa long hypothetical sugar transport membrane protein, 284 aa; fasta scores: opt: 281 z-score: 329.5 E(): 5.2e-11; 26.6% identity in 278 aa overlap and to SW:UGPA_ECOLI (EMBL:X13141) Escherichia coli SN-glycerol-3-phosphate transport system permease protein UgpA, 295 aa; fasta scores: opt: 233 z-score: 274.6 E(): 6e-08; 28.7% identity in 279 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Contains possible N-terminal region signal peptide sequence 1 1 1 8 [Reporter]SCO1681 (14I12)_PCR/7 [Gene]SCO1681/5 NC_003888 SCI30A.02, probable gluconate dehydrogenase, len: 253 aa; similar to many eg. SW:GNO_GLUOX gluconate 5-dehydrogenase from Gluconobacter oxydans (256 aa) fasta scores; opt: 985, z-score: 1113.1, E(): 0, (58.0% identity in 255 aa overlap). Contains Pfam matches to entry PF00106 adh_short, short chain dehydrogenase and to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus, and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature. 1 1 1 7 [Reporter]SCO5160 (15E12)_PCR/6 [Gene]SCO5160/4 NC_003888 SCP8.23, unknown, len: 216 aa 1 1 1 5 [Reporter]SCO3130 (17M8)_PCR/4 [Gene]SCO3130/3 NC_003888 SCE66.09, possible dehydratase, len: 391 aa; similar to many e.g. SW:P31572 (CAIB_ECOLI) L-carnitine dehydratase from Escherichia coli (405 aa) fasta scores; opt: 440, z-score: 478.4, E(): 3.6e-19, 28.7% identity in 401 aa overlap. 1 1 1 4 [Reporter]SCO5048 (18I8)_PCR/3 [Gene]SCO5048/2 NC_003888 SCK7.21, possible secreted protein, len: 174 aa. Contains possible N-terminal signal peptide sequence 1 1 1 3 [Reporter]SCP1.130 (19E8)_PCR/2 [Gene]SCP1.130/1 NC_003888 SCP1.130, unknown, len: 109aa; 4 3 19 16 [Reporter]SCO5634 (7D14)_PCR/0 [Gene]SCO5634/0 NC_003888 SC6A9.33, unknown (pseudogene), len: 45 aa; almost identical to 3' end of TR:E1288124 (EMBL:AJ005260) Pra from Streptomyces ambofaciens plasmid pSAM2 (116 aa); 97.8% identity in 45 aa overlap. Similarity continues from SC6A9.31 after frameshift. No error in the cosmid sequence can be found to account for this,SC6A9.32, unknown (pseudogene), len: 68 aa; identical to 5' end of TR:E1288124 (EMBL:AJ005260) Pra from Streptomyces ambofaciens plasmid pSAM2 (116 aa). Similarity continues in SC6A9.32 after frameshift. No error in the cosmid sequence can be found to account for this 4 2 21 4 [Reporter]SCO6275 (19L3)_PCR/3971 [Gene]SCO6275/3609 NC_003888 SC1G7.01c, probable type I polyketide synthase (fragment), len: >3488 aa; similar to the N-terminal region of TR:Q9L4W3 (EMBL:AF263912) Streptomyces noursei nystatin polyketide synthase NysC, 11096 aa; fasta scores: opt: 7454 Z-score: 6765.5 bits: 1268.6 E(): 0; 45.788% identity in 4285 aa overlap, to TR:O33956 (EMBL:U78289) Streptomyces fradiae tylactone synthase modules 4 & 5 TylG, 3729 aa; fasta scores: opt: 3409 Z-score: 3092.3 bits: 587.4 E(): 1.2e-164; 44.860% identity in 2568 aa overlap and to TR:CAC22145 (EMBL:AL512902) Streptomyces coelicolor SC2C4.04c, 3577 aa; fasta scores: opt: 4872 Z-score: 3412.6 bits: 646.6 E(): 2.3e-184; 58.281% identity in 2560 aa overlap. Contains Pfam matches to entries 3x PF00109 ketoacyl-synt, Beta-ketoacyl synthase, 3x PF02801 ketoacyl-synt_C, Beta-ketoacyl synthase, 3x PF00698 Acyl_transf, Acyl transferase domain, PF00550 pp-binding, Phosphopantetheine attachment site, PF00550 pp-binding, Phosphopantetheine attachment site and matches to Prosite entries PS00012 Phosphopantetheine attachment site and PS00606 Beta-ketoacyl synthases active site,SC2C4.05c, probable type I polyketide synthase (fragment), len: >1107 aa; similar to C-terminal region of TR:O33955 (EMBL:U78289) Streptomyces fradiae tylactone synthase module 3 TylG, 1864 aa; fasta scores: opt: 2105 z-score: 2202.2 E(): 0; 46.0% identity in 1161 aa overlap. Also highly similar, in this same cosmid, to SC2C4.03c and SC2C4.04c. Contains Pfam matches to entries PF00698 Acyl_transf, Acyl transferase domain and PF00550 pp-binding, Phosphopantetheine attachment site and matches to Prosite entries PS00216 Sugar transport proteins signature 1 and PS00012 Phosphopantetheine attachment site 4 2 21 3 [Reporter]SCO4896 (20H3)_PCR/3970 [Gene]SCO4896/3608 NC_003888 2SCK8.22c, probable transport integral membrane protein, len: 436 aa; similar to TR:AAG56583 (EMBL:AE005383) Escherichia coli O157:H7 putative transport protein YnfM, 417 aa; fasta scores: opt: 971 z-score: 995.9 E(): 0; 38.8% identity in 418 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and matches to Prosite entries PS00216 Sugar transport proteins signature 1 and PS00017 ATP/GTP-binding site motif A (P-loop. Also contains possible hydrophobic membrane spanning regions) 4 2 21 2 [Reporter]SCO7477 (20P23)_PCR/3969 [Gene]SCO7477/3607 NC_003888 SCBAC17A6.10, possible membrane protein, len: 497 aa: no significant database matches. Contains a possible hydrophobic membrane spanning region and Prosite match to PS00216 Sugar transport proteins signature 1. 4 2 20 22 [Reporter]SCO1353 (1P19)_PCR/3967 [Gene]SCO1353/3606 NC_003888 2SCG61.35, possible transcriptional regulator, len: 343 aa; similar to many from proposed regulators from streptomyces e.g. TR:Q9ZBT5 (EMBL:AL034446) putative regulatory protein from Streptomyces coelicolor (327 aa) fasta scores; opt: 445, z-score: 498.6, E(): 2.7e-20, 31.7% identity in 325 aa overlap. Contains helix-turn-helix motif (residue 298-319) 4 2 20 21 [Reporter]SCO7432 (2L19)_PCR/3966 [Gene]SCO7432/3605 NC_003888 SC6D11.28c, mprA2, secreted extracellular small neutral protease (EC 3.4.24.-), len: 227 aa. Identical to Streptomyces lividans SW:SNPA_STRLI(EMBL:M81703) extracellular small neutral protease precursor, SnpA (227 aa), fasta scores opt: 1483 z-score: 1546.5 E(): 0 100.0% identity in 227 aa overlap. Also highly similar to Streptomyces coelicolor SW:SNPA_STRCO(EMBL:Z11929) extracellular small neutral protease precursor, MprA (215 aa), fasta scores opt: 854 z-score: 896.8 E():0 65.6% identity in 218 aa overlap. Contains a possible N-terminal signal sequence. 4 2 20 20 [Reporter]SCO7663 (3H19)_PCR/3965 [Gene]SCO7663/3604 NC_003888 SC10F4.36c, conserved hypothetical protein, len: 94 aa; similar to TR:O05862 (EMBL:Z95120) Mycobacterium tuberculosis hypothetical 11.0 kDa protein MTCY07D11.22c, 101 aa; fasta scores: opt: 180 z-score: 238.5 E(): 9.5e-06; 34.8% identity in 92 aa overlap 4 2 20 19 [Reporter]SCO5614 (4D19)_PCR/3964 [Gene]SCO5614/3603 NC_003888 SC2E1.31, possible prophage transcriptional regulator, len: 63 aa; similar to the N-terminus of many e.g. TR:E264367 (EMBL:X87420) Salmonella typhimurium bacteriophage ES18 gene C2 (216 aa), fasta scores; opt: 133 z-score: 207.9E(): 0.00024, 35.8% identity in 53 aa overlap. Contains probable helix-turn-helix motif at aa 17 to 38 (Score 1786, +5.27 SD) 4 1 3 5 [Reporter]SCO0251 (17H12)_PCR/1560 [Gene]SCO0251/1429 NC_003888 SCJ9A.30c, unknown, len: 94aa; prediction based on Hidden Markov Model. 4 2 20 18 [Reporter]SCO7687 (5P15)_PCR/3963 [Gene]SCO7687/3602 NC_003888 SC4C2.22, probable thioesterase, len: 277 aa; similar to SW:PABT_STRGR (EMBL:M93058) Streptomyces griseus probable cadicidin biosynthesis thioesterase (EC 3.1.2.-), 361 aa; fasta scores: opt: 638 z-score: 760.5 E(): 0; 40.4% identity in 277 aa overlap. Contains Pfam match to entry PF00975 Thioesterase, Thioesterase domain 4 1 3 4 [Reporter]SCO0147 (18D12)_PCR/1559 [Gene]SCO0147/1428 NC_003888 SCJ33.11, possible transmembrane transport protein, len: 163 aa. Weakly similar to several including: Aquifex aeolicus TR:O66755 (EMBL; AE000690) MG(2+) transport ATPase (225 aa), fasta scores opt: 110 z-score: 142.5 E(): 1.4 26.7% identity in 146 aa overlap and Bacillus subtilis TR:O31833 (EMBL; Z99114) YoaS protein (160 aa), fasta scores opt:109 z-score: 143.2 E(): 1.3 25.0% identity in 148 aa overlap. Contains a Prosite hit to PS00211 ABC transporters family signature and Contains possible membrane spanning hydrophobic domains. 4 2 20 17 [Reporter]SCO2189 (6L15)_PCR/3962 [Gene]SCO2189/3601 NC_003888 SC5F7.12c, possible acetyltransferase, len: 169 aa; similar to SW:PTCR_ALCFA phosphinothricin acetyltransferase from Alcaligenes faecalis (197 aa) fasta scores; opt: 178, z-score: 226.5, E(): 2.8e-05, (36.0% identity in 136 aa overlap) and TR:O33583 (EMBL:S60706) nourseothricin acetyltransferase from Streptomyces noursei (190 aa) fasta scores; opt: 140, z-score: 180.8, E(): 0.0098, (29.3% identity in 150 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 4 1 3 2 [Reporter]SCO7521 (20H8)_PCR/1557 [Gene]SCO7521/1427 NC_003888 SCBAC25F8.13c, possible secreted peptidase, len: 371 aa; similar to TR:P94288 (EMBL:D86380) Bacillus cereus alkaline D-peptidase, Adp, 388 aa; fasta scores: opt: 430 Z-score: 448.6 bits: 91.7 E(): 2.2e-17; 34.663% identity in 326 aa overlap. Contains possible N-terminal region signal peptide sequence 4 2 20 16 [Reporter]SCO6173 (7H15)_PCR/3961 [Gene]SCO6173/3600 NC_003888 SC6C5.09, probable permease, len: 485aa; similar to many eg. SW:Y326_METJA hypothetical protein from Methanococcus jannaschii (436 aa) fasta scores; opt: 517, z-score: 1019.3, E(): 0, (37.2% identity in 454 aa overlap). Contains Pfam match to entry PF00860 xan_ur_permease, Xanthine /uracil permeases family, score 43.50, E-value 4.9e-09. 4 1 2 22 [Reporter]SCO5363 (1H4)_PCR/1555 [Gene]SCO5363/1426 NC_003888 2SC6G5.07, unknown, len: 166 aa 4 1 2 21 [Reporter]SCO0773 (2D4)_PCR/1554 [Gene]SCO0773/1425 NC_003888 3SCF60.05c, soyB2, probable ferredoxin, len: 66 aa; identical to TR:O85696 (EMBL:AF072709) Streptomyces lividans putative ferredoxin, 66 aa and similar to SW:FERS_STRGR (EMBL:X63601) Streptomyces griseus ferredoxin SoyB, 65 aa; fasta scores: opt: 269 z-score: 369.1 E(): 5e-13; 61.9% identity in 63 aa overlap 4 1 2 20 [Reporter]SCO4571 (2P24)_PCR/1553 [Gene]SCO4571/1424 NC_003888 SCD16A.12c, nuoJ, NADH dehydrogenase subunit, len: 285 aa; similar to many e.g. TR:P95172 (EMBL:Z83867) NuoJ, NADH dehydrogenase subunit from Mycobacterium tuberculosis (262 aa) fasta scores; opt: 991, z-score: 1118.0, E(): 0, (63.4% identity in 243 aa overlap) and SW:NUOJ_ECOLI, NADH dehydrogenase subunit NuoJ from Escherichia coli (184 aa) fasta scores; opt: 263, z-score: 306.0, E(): 1e-09, (32.0% identity in 169 aa overlap). Contains Pfam match to entry PF00499 oxidored_q3, NADH-ubiquinone/plastoquinone oxidoreductase chain 6. Contains possible membrane spanning hydrophobic regions 4 1 2 19 [Reporter]SCO6149 (3L24)_PCR/1552 [Gene]SCO6149/1423 NC_003888 SC1A9.13, possible nucleotide binding protein, len: 366 aa; similar to several hypotheticals eg. YJEQ_HAEIN (EMBL:U32844) hypothetical protein HI1714 from Haemophilus influenzae (346 aa) fasta scores; opt:472, z-score: 564.4, E(): 3.7e-24, (31.0% identity in 313 aa overlap). Contains PS00017 ATP /GTP-binding site motif A (P-loop). 4 1 2 18 [Reporter]SCO6206 (4H24)_PCR/1551 [Gene]SCO6206/1422 NC_003888 SC2G5.27c, possible oxidoreductase, len: 279 aa; similar to many, mostly hypotheticals including TR:O50580 (EMBL :AB000361) D-tagatose 3-epimerase from Pseudomonas cichorii (290 aa) fasta scores; opt: 164, z-score:193.0,E(): 0.0018 ,(26.8% identity in 142 aa overlap). Most similar to SW:GIP_ECOLI glyoxylate-induced protein from Escherichia coli (258 aa) fasta scores; opt: 411, z-score:473.6, E(): 4.3e-19, (35.0% identity in 266 aa overlap). As with the Escherichia coli homologue, this CDS is located close to the CDS for gcl (SC2G5.22). 4 1 2 17 [Reporter]SCO6559 (5D24)_PCR/1550 [Gene]SCO6559/1421 NC_003888 SC4B5.09c, respiratory chain oxidoreductase, len: 286 aa; similar to the N-terminal region of many proteins involved in electron transfer e.g. TR:P77908 (EMBL:U73807) FdhA formate dehydrogenase alpha subunit from Moorella thermoacetica (893 aa), fasta scores; opt: 685, z-score: 791.8, E(): 0, (38.4% identity in 284 aa overlap). Contains PS00551 Prokaryotic molybdopterin oxidoreductases signature 1, PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature, PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature, Pfam match to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains, score 40.00, E-value 2.4e-10 and Pfam match to entry PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains, score -5.70, E-value 0.11. Considering the corresponding homologies, this CDS may have previously been located directly upsteam of SC4B5.11c 4 1 2 16 [Reporter]SCO7310 (6P20)_PCR/1549 [Gene]SCO7310/1420 NC_003888 SC5F8.20c, possible regulatory protein, len: 151 aa. Similar in parts to Pseudomonas sp.TR:O52578 (EMBL:AF040987) putative FNR-like regulator, NnrR (223 aa), fasta scores opt: 161 z-score: 223.3 E(): 5.7e-05 25.2% identity in 131 aa overlap and Streptomyces coelicolor TR:Q9RIZ7 (EMBL:AL109962) possible regulator protein, SCJ1.17 (190 aa), fasta scores opt: 500 z-score: 675.2 E(): 3.8e-30 49.7% identity in 151 aa overlap. Contains a Pfam match to entry PF00027 cNMP_binding, Cyclic nucleotide-binding domain. 4 1 2 15 [Reporter]SCO4421 (7L20)_PCR/1548 [Gene]SCO4421/1419 NC_003888 SC6F11.19c, possible tetR-family transcriptional regulatory protein, len: 211 aa; similar to TR:Q9RD77 (EMBL:AL136502) Streptomyces coelicolor putative tetR-family regulatory protein SCF43.11c, 205 aa; fasta scores: opt: 156 z-score: 196.9 E(): 0.0018; 28.2% identity in 131 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and a possible helix-turn-helix motif at residues 36..57 (+4.34 SD) 4 1 2 14 [Reporter]SCO6884 (8H20)_PCR/1547 [Gene]SCO6884/1418 NC_003888 SC7F9.36c, possible secreted protein, len: 377 aa. Highly similar to Streptomyces coelicolor TR:Q9X867(EMBL:AL049661) putative lipoprotein, SCE134.12 (381 aa), fasta scores opt: 1374 z-score: 1462.4 E():0 56.0% identity in 384 aa overlap. Contains a Pfam match to entry PF00877 NLPC_P60, NLP/P60 family and a possible N-terminal signal sequence. 4 1 2 13 [Reporter]SCO7162 (9D20)_PCR/1546 [Gene]SCO7162/1417 NC_003888 SC9A4.24c, possible oxidoreductase, len: 307 aa; similar to TR:Q9RJW1 (EMBL:AL117387) Streptomyces coelicolor possible oxidoreductase SCF41.08, 334 aa; fasta scores: opt: 705 z-score: 808.0 E(): 0; 48.1% identity in 314 aa overlap 4 1 2 12 [Reporter]SCO1947 (10P16)_PCR/1545 [Gene]SCO1947/1416 NC_003888 SCC54.07c, gap1, glyceraldehyde-3-phosphate dehydrogenase, len: 336aa; member of a highly conserved family of proteins eg. SW:G3P_BACST gap, glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus (334 aa) fasta scores; opt: 1477, z-score: 1657.1, E(): 0, (67.9% identity in 333 aa overlap). Contains Pfam match to entry PF00044 gpdh, glyceraldehyde 3-phosphate dehydrogenases, score 725.60, E-value 2.2e-214. 4 1 2 11 [Reporter]SCO4516 (11L16)_PCR/1544 [Gene]SCO4516/1415 NC_003888 SCD35.23c, unknown, len: 119 aa 4 1 2 10 [Reporter]SCO4273 (12H16)_PCR/1543 [Gene]SCO4273/1414 NC_003888 SCD95A.06c, possible metallopeptidase, len: 473 aa; similar to TR:Q9X7P2 (EMBL:AL049587) Streptomyces coelicolor putative metallopeptidase SC5F2A.19c, 506 aa; fasta scores: opt: 1253 z-score: 1353.9 E(): 0; 42.6% identity in 479 aa overlap and to N-terminal domain of SW:AMPN_LACHE (EMBL:U08224) Lactobacillus helveticus aminopeptidase N (EC 3.4.11.2), PepN, 844 aa; fasta scores: opt: 310 z-score: 333.8 E(): 3.9e-11; 27.9% identity in 451 aa overlap. Contains Pfam match to entry PF01433 Peptidase_M1, Peptidase family M1 and matches to Prosite entries PS00013 Prokaryotic membrane lipoprotein lipid attachment site (correctly situated) and PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Contains also a possible N-terminal region signal peptide sequence 4 1 2 9 [Reporter]SCO2981 (13D16)_PCR/1542 [Gene]SCO2981/1413 NC_003888 SCE50.09, possible glycosyl transferase, len: 765 aa; similar to TR:CAB66267 (EMBL:AL136518) Streptomyces coelicolor putative glycosyl transferase (fragment) SCC123.27, 743 aa; fasta scores: opt: 2334 z-score: 2578.7 E():0; 50.4% identity in 748 aa overlap and to SW:TAGF_BACSU (EMBL:X15200) Bacillus subtilis teichoic acid biosynthesis protein F, TagF, 746 aa; fasta scores: opt: 319 z-score: 350.8 E(): 4.3e-12; 27.9% identity in 376 aa overlap. Also similar to Streptomyces coelicolor SCE50.10, 736 aa; fasta scores: opt: 1837 z-score: 1644.2 E(): 0; 45.2% identity in 756 aa overlap and SCE50.11, 745 aa ;fasta scores: opt: 2095 z-score: 1875.1 E(): 0; 49.0% identity in 764 aa overlap. Contains Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases 3 1 19 1 [Reporter]P7 (CtrlK20)_PCR/1404 1 4 12 4 [Reporter]no spot (18E17)_PCR/6292 1 4 12 1 [Reporter]23s rRNA 1:64 (CtrlA9)_PCR/6289 1 3 4 1 [Reporter]16s rRNA 1:4 (CtrlA6)_PCR/4098 1 4 11 4 [Reporter]no spot (18A17)_PCR/6270 1 1 10 1 [Reporter]23s rRNA 1:4 (CtrlI12)_PCR/198 1 1 9 21 [Reporter]no spot (2M8)_PCR/196 1 4 11 1 [Reporter]23s rRNA 1:16 (CtrlE9)_PCR/6267 1 1 9 8 [Reporter]no spot (14I20)_PCR/183 1 3 3 2 [Reporter]no spot (20E6)_PCR/4077 1 3 3 1 [Reporter]16s rRNA 1:1 (CtrlE6)_PCR/4076 1 3 2 20 [Reporter]no spot (2M22)_PCR/4073 1 1 9 1 [Reporter]23s rRNA 1:1 (CtrlM12)_PCR/176 1 4 10 1 [Reporter]23s rRNA 1:4 (CtrlI9)_PCR/6245 1 3 2 1 [Reporter]16s carry-over (CtrlI6)_PCR/4054 1 1 8 5 [Reporter]no spot (17I16)_PCR/158 1 1 8 1 [Reporter]23s carry-over (CtrlA16)_PCR/154 1 1 7 20 [Reporter]no spot (3A8)_PCR/151 1 1 7 19 [Reporter]no spot (4M4)_PCR/150 1 4 9 2 [Reporter]no spot (20M9)_PCR/6224 1 4 9 1 [Reporter]23s rRNA 1:1 (CtrlM9)_PCR/6223 1 4 8 20 [Reporter]no spot (3E5)_PCR/6220 1 1 7 11 [Reporter]no spot (11M20)_PCR/142 1 3 1 1 [Reporter]Cy3 lights (CtrlE22)_PCR/4033 1 1 7 1 [Reporter]16s rRNA 1:256 (CtrlE4)_PCR/132 1 4 8 1 [Reporter]23s carry-over (CtrlA13)_PCR/6201 1 1 6 14 [Reporter]no spot (8E24)_PCR/123 4 2 23 1 [Reporter]Cy5 lights (CtrlP23)_PCR/4012 1 1 6 1 [Reporter]16s rRNA 1:64 (CtrlI4)_PCR/110 4 4 21 1 [Reporter]P10 (CtrlD21)_PCR/7999 4 4 20 21 [Reporter]no spot (2L17)_PCR/7997 4 4 20 17 [Reporter]no spot (6L13)_PCR/7993 1 1 5 22 [Reporter]no spot (1A8)_PCR/109 4 4 20 1 [Reporter]P8 (CtrlP17)_PCR/7977 4 4 19 20 [Reporter]no spot (3D17)_PCR/7974 4 3 12 10 [Reporter]no spot (12P22)_PCR/5795 4 4 19 14 [Reporter]no spot (9L9)_PCR/7968 4 3 12 1 [Reporter]23s rRNA 1:64 (CtrlD10)_PCR/5786 4 3 11 17 [Reporter]no spot (6H6)_PCR/5780 4 4 19 1 [Reporter]P7 (CtrlL17)_PCR/7955 4 2 4 4 [Reporter]no spot (18H11)_PCR/3597 4 2 4 2 [Reporter]no spot (20L7)_PCR/3595 4 2 4 1 [Reporter]16s rRNA 1:4 (CtrlD7)_PCR/3594 4 2 3 20 [Reporter]no spot (3D3)_PCR/3591 4 4 18 15 [Reporter]no spot (8L9)_PCR/7947 4 3 11 1 [Reporter]23s rRNA 1:16 (CtrlH10)_PCR/5764 4 2 3 15 [Reporter]no spot (7P19)_PCR/3586 4 4 18 6 [Reporter]no spot (17H1)_PCR/7938 4 4 18 1 [Reporter]P6 (CtrlH17)_PCR/7933 4 2 3 5 [Reporter]no spot (17H11)_PCR/3576 4 2 3 4 [Reporter]no spot (18D11)_PCR/3575 4 2 3 1 [Reporter]16s rRNA 1:1 (CtrlH7)_PCR/3572 4 2 2 22 [Reporter]no spot (1H3)_PCR/3571 3 1 18 9 [Reporter]no spot (14C8)_PCR/1390 4 3 10 1 [Reporter]23s rRNA 1:4 (CtrlL10)_PCR/5742 4 3 9 21 [Reporter]no spot (2P6)_PCR/5740 3 1 18 5 [Reporter]no spot (18C4)_PCR/1386 3 1 18 1 [Reporter]P6 (CtrlG20)_PCR/1382 4 4 17 1 [Reporter]P5 (CtrlD17)_PCR/7911 4 3 9 14 [Reporter]no spot (9D2)_PCR/5733 4 3 9 12 [Reporter]no spot (10L22)_PCR/5731 4 2 2 1 [Reporter]16s carry-over (CtrlL7)_PCR/3550 4 4 16 15 [Reporter]no spot (8D9)_PCR/7903 4 3 9 1 [Reporter]23s rRNA 1:1 (CtrlP10)_PCR/5720 3 1 17 1 [Reporter]P5 (CtrlC20)_PCR/1360 4 3 8 21 [Reporter]no spot (2L6)_PCR/5718 4 3 8 14 [Reporter]no spot (8P22)_PCR/5711 4 2 1 10 [Reporter]no spot (12D15)_PCR/3537 4 2 1 1 [Reporter]Cy3 lights (CtrlH23)_PCR/3529 3 1 16 1 [Reporter]P4 (CtrlO16)_PCR/1338 3 2 23 5 [Reporter]no spot (18G7)_PCR/3511 3 2 23 1 [Reporter]Cy5 lights (CtrlO23)_PCR/3508 3 1 15 13 [Reporter]no spot (10G8)_PCR/1328 3 1 15 1 [Reporter]P3 (CtrlK16)_PCR/1316 3 1 14 13 [Reporter]no spot (10C8)_PCR/1306 1 4 7 1 [Reporter]16s rRNA 1:256 (CtrlE1)_PCR/6179 1 4 6 1 [Reporter]16s rRNA 1:64 (CtrlI1)_PCR/6157 1 4 5 2 [Reporter]no spot (20M5)_PCR/6136 1 4 5 1 [Reporter]16s rRNA 1:16 (CtrlM1)_PCR/6135 1 4 4 1 [Reporter]16s rRNA 1:4 (CtrlA5)_PCR/6113 4 4 16 5 [Reporter]no spot (17L21)_PCR/7893 4 4 16 1 [Reporter]P4 (CtrlP13)_PCR/7889 4 4 15 16 [Reporter]no spot (7D9)_PCR/7882 4 3 8 1 [Reporter]23s carry-over (CtrlD14)_PCR/5698 4 3 7 21 [Reporter]no spot (2H6)_PCR/5696 4 3 7 20 [Reporter]no spot (3D6)_PCR/5695 4 4 15 1 [Reporter]P3 (CtrlL13)_PCR/7867 4 4 14 15 [Reporter]no spot (8L5)_PCR/7859 4 3 7 1 [Reporter]16s rRNA 1:256 (CtrlH2)_PCR/5676 3 2 22 9 [Reporter]no spot (14C11)_PCR/3494 3 2 22 7 [Reporter]no spot (16K7)_PCR/3492 4 4 14 1 [Reporter]P1 (CtrlH13)_PCR/7845 4 3 6 12 [Reporter]no spot (10P18)_PCR/5665 4 3 6 10 [Reporter]no spot (12H18)_PCR/5663 3 2 22 2 [Reporter]50% DMSO (CtrlC3)_PCR/3487 3 2 22 1 [Reporter]Cy3 lights carry-over (CtrlK23)_PCR/3486 4 3 6 1 [Reporter]16s rRNA 1:64 (CtrlL2)_PCR/5654 3 2 21 10 [Reporter]no spot (13C11)_PCR/3473 3 1 14 1 [Reporter]P1 (CtrlG16)_PCR/1294 4 4 13 1 [Reporter]23s rRNA 1:256 (CtrlP5)_PCR/7823 4 3 5 17 [Reporter]no spot (5P22)_PCR/5648 3 2 21 1 [Reporter]P10 (CtrlC23)_PCR/3464 3 1 13 16 [Reporter]no spot (7K8)_PCR/1287 4 4 12 15 [Reporter]no spot (8D5)_PCR/7815 4 4 12 12 [Reporter]no spot (11H1)_PCR/7812 4 3 5 1 [Reporter]16s rRNA 1:16 (CtrlP2)_PCR/5632 3 1 13 1 [Reporter]23s rRNA 1:256 (CtrlO8)_PCR/1272 4 4 12 4 [Reporter]no spot (18H17)_PCR/7804 4 4 12 1 [Reporter]23s rRNA 1:64 (CtrlD9)_PCR/7801 4 3 4 11 [Reporter]no spot (11D18)_PCR/5620 3 2 20 1 [Reporter]P8 (CtrlO19)_PCR/3442 4 3 4 1 [Reporter]16s rRNA 1:4 (CtrlD6)_PCR/5610 3 2 19 14 [Reporter]no spot (9K11)_PCR/3433 3 1 12 9 [Reporter]no spot (13K24)_PCR/1258 3 1 12 1 [Reporter]23s rRNA 1:64 (CtrlC12)_PCR/1250 4 3 3 19 [Reporter]no spot (3P22)_PCR/5606 3 2 19 3 [Reporter]no spot (19O23)_PCR/3422 3 2 19 1 [Reporter]P7 (CtrlK19)_PCR/3420 3 1 11 19 [Reporter]no spot (4O8)_PCR/1246 3 2 18 5 [Reporter]no spot (18C3)_PCR/3402 3 1 11 1 [Reporter]23s rRNA 1:16 (CtrlG12)_PCR/1228 3 1 10 22 [Reporter]no spot (1G12)_PCR/1227 3 1 10 1 [Reporter]23s rRNA 1:4 (CtrlK12)_PCR/1206 2 2 22 17 [Reporter]no spot (6B19)_PCR/2998 2 2 22 14 [Reporter]no spot (9F15)_PCR/2995 2 2 22 5 [Reporter]no spot (18B7)_PCR/2986 2 2 22 2 [Reporter]50% DMSO (CtrlB3)_PCR/2983 2 2 22 1 [Reporter]Cy3 lights carry-over (CtrlJ23)_PCR/2982 1 4 3 9 [Reporter]no spot (13E13)_PCR/6099 1 4 3 1 [Reporter]16s rRNA 1:1 (CtrlE5)_PCR/6091 2 2 21 1 [Reporter]P10 (CtrlB23)_PCR/2960 1 4 2 1 [Reporter]16s carry-over (CtrlI5)_PCR/6069 2 2 20 1 [Reporter]P8 (CtrlN19)_PCR/2938 1 4 1 1 [Reporter]Cy3 lights (CtrlE21)_PCR/6048 2 2 19 1 [Reporter]P7 (CtrlJ19)_PCR/2916 4 3 23 8 [Reporter]no spot (15D10)_PCR/6033 2 2 18 9 [Reporter]no spot (14B7)_PCR/2902 4 3 23 1 [Reporter]Cy5 lights (CtrlP22)_PCR/6027 4 3 22 2 [Reporter]50% DMSO (CtrlD2)_PCR/6006 4 3 22 1 [Reporter]Cy3 lights carry-over (CtrlL22)_PCR/6005 4 4 11 15 [Reporter]no spot (8P1)_PCR/7793 4 4 11 3 [Reporter]no spot (19P13)_PCR/7781 4 4 11 1 [Reporter]23s rRNA 1:16 (CtrlH9)_PCR/7779 4 3 3 9 [Reporter]no spot (13H14)_PCR/5596 4 3 3 1 [Reporter]16s rRNA 1:1 (CtrlH6)_PCR/5588 4 4 10 1 [Reporter]23s rRNA 1:4 (CtrlL9)_PCR/7757 4 3 2 9 [Reporter]no spot (13D14)_PCR/5574 3 2 18 1 [Reporter]P6 (CtrlG19)_PCR/3398 3 2 17 21 [Reporter]no spot (2O15)_PCR/3396 4 3 2 1 [Reporter]16s carry-over (CtrlL6)_PCR/5566 4 3 1 20 [Reporter]no spot (2L22)_PCR/5563 4 4 9 1 [Reporter]23s rRNA 1:1 (CtrlP9)_PCR/7735 4 4 8 20 [Reporter]no spot (3H5)_PCR/7732 3 2 17 1 [Reporter]P5 (CtrlC19)_PCR/3376 4 3 1 1 [Reporter]Cy3 lights (CtrlH22)_PCR/5545 3 2 16 15 [Reporter]no spot (8C11)_PCR/3368 3 1 9 1 [Reporter]23s rRNA 1:1 (CtrlO12)_PCR/1184 4 4 8 1 [Reporter]23s carry-over (CtrlD13)_PCR/7713 4 4 7 20 [Reporter]no spot (3D5)_PCR/7710 3 3 23 8 [Reporter]no spot (15C10)_PCR/5530 3 2 16 1 [Reporter]P4 (CtrlO15)_PCR/3354 4 4 7 12 [Reporter]no spot (10D21)_PCR/7702 4 4 7 11 [Reporter]no spot (11P17)_PCR/7701 3 3 23 1 [Reporter]Cy5 lights (CtrlO22)_PCR/5524 3 1 8 1 [Reporter]23s carry-over (CtrlC16)_PCR/1162 3 3 22 17 [Reporter]no spot (6C18)_PCR/5518 3 3 22 15 [Reporter]no spot (8K14)_PCR/5516 3 3 22 9 [Reporter]no spot (14C10)_PCR/5510 3 2 15 1 [Reporter]P3 (CtrlK15)_PCR/3332 3 1 7 16 [Reporter]no spot (7C4)_PCR/1155 3 1 7 13 [Reporter]no spot (9G24)_PCR/1152 3 3 22 2 [Reporter]50% DMSO (CtrlC2)_PCR/5503 3 3 22 1 [Reporter]Cy3 lights carry-over (CtrlK22)_PCR/5502 3 2 14 19 [Reporter]no spot (4K11)_PCR/3328 3 1 7 1 [Reporter]16s rRNA 1:256 (CtrlG4)_PCR/1140 3 2 14 1 [Reporter]P1 (CtrlG15)_PCR/3310 3 1 6 13 [Reporter]no spot (9C24)_PCR/1130 3 1 6 12 [Reporter]no spot (10O20)_PCR/1129 3 2 13 15 [Reporter]no spot (8G7)_PCR/3302 3 1 6 1 [Reporter]16s rRNA 1:64 (CtrlK4)_PCR/1118 3 1 5 12 [Reporter]no spot (10K20)_PCR/1107 3 1 5 7 [Reporter]no spot (15G16)_PCR/1102 2 2 18 3 [Reporter]no spot (19J23)_PCR/2896 2 2 18 1 [Reporter]P6 (CtrlF19)_PCR/2894 2 2 17 19 [Reporter]no spot (4F15)_PCR/2890 2 2 17 1 [Reporter]P5 (CtrlB19)_PCR/2872 2 2 16 1 [Reporter]P4 (CtrlN15)_PCR/2850 2 2 15 19 [Reporter]no spot (4N11)_PCR/2846 2 2 15 15 [Reporter]no spot (8N7)_PCR/2842 2 2 15 1 [Reporter]P3 (CtrlJ15)_PCR/2828 2 2 14 10 [Reporter]no spot (13F3)_PCR/2815 2 2 14 1 [Reporter]P1 (CtrlF15)_PCR/2806 4 4 7 1 [Reporter]16s rRNA 1:256 (CtrlH1)_PCR/7691 4 4 6 10 [Reporter]no spot (12H17)_PCR/7678 4 4 6 1 [Reporter]16s rRNA 1:64 (CtrlL1)_PCR/7669 4 4 5 19 [Reporter]no spot (4H1)_PCR/7665 3 3 21 1 [Reporter]P10 (CtrlC22)_PCR/5480 4 4 5 2 [Reporter]no spot (20P5)_PCR/7648 4 4 5 1 [Reporter]16s rRNA 1:16 (CtrlP1)_PCR/7647 4 4 4 16 [Reporter]no spot (6H21)_PCR/7640 3 2 13 1 [Reporter]23s rRNA 1:256 (CtrlO7)_PCR/3288 4 4 4 14 [Reporter]no spot (8P17)_PCR/7638 3 3 20 1 [Reporter]P8 (CtrlO18)_PCR/5458 3 1 5 4 [Reporter]no spot (18K12)_PCR/1099 3 1 5 2 [Reporter]no spot (20O8)_PCR/1097 3 1 5 1 [Reporter]16s rRNA 1:16 (CtrlO4)_PCR/1096 3 1 4 20 [Reporter]no spot (3G4)_PCR/1093 4 4 4 1 [Reporter]16s rRNA 1:4 (CtrlD5)_PCR/7625 3 2 12 4 [Reporter]no spot (18G19)_PCR/3269 3 2 12 1 [Reporter]23s rRNA 1:64 (CtrlC11)_PCR/3266 4 4 3 17 [Reporter]no spot (5H21)_PCR/7619 4 4 3 12 [Reporter]no spot (10D17)_PCR/7614 3 3 19 1 [Reporter]P7 (CtrlK18)_PCR/5436 3 2 11 10 [Reporter]no spot (12K23)_PCR/3253 3 1 4 1 [Reporter]16s rRNA 1:4 (CtrlC8)_PCR/1074 4 4 3 1 [Reporter]16s rRNA 1:1 (CtrlH5)_PCR/7603 3 2 11 1 [Reporter]23s rRNA 1:16 (CtrlG11)_PCR/3244 3 2 10 19 [Reporter]no spot (4K7)_PCR/3240 3 3 18 5 [Reporter]no spot (18C2)_PCR/5418 3 3 18 1 [Reporter]P6 (CtrlG18)_PCR/5414 3 1 3 2 [Reporter]no spot (20G8)_PCR/1053 3 1 3 1 [Reporter]16s rRNA 1:1 (CtrlG8)_PCR/1052 3 1 2 21 [Reporter]no spot (2C4)_PCR/1050 3 3 17 11 [Reporter]no spot (12G6)_PCR/5402 3 2 10 1 [Reporter]23s rRNA 1:4 (CtrlK11)_PCR/3222 3 2 9 21 [Reporter]no spot (2O7)_PCR/3220 3 1 2 1 [Reporter]16s carry-over (CtrlK8)_PCR/1030 3 2 9 6 [Reporter]no spot (16C19)_PCR/3205 3 2 9 1 [Reporter]23s rRNA 1:1 (CtrlO11)_PCR/3200 3 1 1 16 [Reporter]no spot (6K20)_PCR/1023 3 1 1 4 [Reporter]no spot (18K8)_PCR/1011 2 3 22 2 [Reporter]50% DMSO (CtrlB2)_PCR/4999 2 3 22 1 [Reporter]Cy3 lights carry-over (CtrlJ22)_PCR/4998 3 1 1 1 [Reporter]Cy3 lights (CtrlG24)_PCR/1009 2 3 21 1 [Reporter]P10 (CtrlB22)_PCR/4976 2 3 20 9 [Reporter]no spot (14J6)_PCR/4962 2 2 13 1 [Reporter]23s rRNA 1:256 (CtrlN7)_PCR/2784 2 3 20 1 [Reporter]P8 (CtrlN18)_PCR/4954 2 2 12 12 [Reporter]no spot (11F3)_PCR/2773 2 3 19 18 [Reporter]no spot (5J14)_PCR/4949 2 3 19 17 [Reporter]no spot (6F14)_PCR/4948 1 1 1 2 [Reporter]no spot_PCR/8064 2 1 1 2 [Reporter]no spot_PCR/8064 3 1 1 2 [Reporter]no spot_PCR/8064 4 1 1 2 [Reporter]no spot_PCR/8064 1 2 1 2 [Reporter]no spot_PCR/8064 2 2 1 2 [Reporter]no spot_PCR/8064 3 2 1 2 [Reporter]no spot_PCR/8064 4 2 1 2 [Reporter]no spot_PCR/8064 1 3 1 2 [Reporter]no spot_PCR/8064 2 3 1 2 [Reporter]no spot_PCR/8064 3 3 1 2 [Reporter]no spot_PCR/8064 4 3 1 2 [Reporter]no spot_PCR/8064 1 4 1 2 [Reporter]no spot_PCR/8064 2 4 1 2 [Reporter]no spot_PCR/8064 3 4 1 2 [Reporter]no spot_PCR/8064 4 4 1 2 [Reporter]no spot_PCR/8064 1 1 23 2 [Reporter]no spot_PCR/8064 2 1 23 2 [Reporter]no spot_PCR/8064 3 1 23 2 [Reporter]no spot_PCR/8064 4 1 23 2 [Reporter]no spot_PCR/8064 1 2 23 2 [Reporter]no spot_PCR/8064 2 2 23 2 [Reporter]no spot_PCR/8064 3 2 23 2 [Reporter]no spot_PCR/8064 4 2 23 2 [Reporter]no spot_PCR/8064 1 3 23 2 [Reporter]no spot_PCR/8064 2 3 23 2 [Reporter]no spot_PCR/8064 3 3 23 2 [Reporter]no spot_PCR/8064 4 3 23 2 [Reporter]no spot_PCR/8064 1 4 23 2 [Reporter]no spot_PCR/8064 2 4 23 2 [Reporter]no spot_PCR/8064 3 4 23 2 [Reporter]no spot_PCR/8064 4 4 23 2 [Reporter]no spot_PCR/8064 2 2 12 1 [Reporter]23s rRNA 1:64 (CtrlB11)_PCR/2762 2 3 19 1 [Reporter]P7 (CtrlJ18)_PCR/4932 2 3 18 13 [Reporter]no spot (10B10)_PCR/4922 4 4 23 1 [Reporter]Cy5 lights (CtrlP21)_PCR/8043 2 2 11 1 [Reporter]23s rRNA 1:16 (CtrlF11)_PCR/2740 2 3 18 1 [Reporter]P6 (CtrlF18)_PCR/4910 2 2 10 15 [Reporter]no spot (8J3)_PCR/2732 4 4 22 5 [Reporter]no spot (18D5)_PCR/8025 4 4 22 2 [Reporter]50% DMSO (CtrlD1)_PCR/8022 4 4 22 1 [Reporter]Cy3 lights carry-over (CtrlL21)_PCR/8021 4 4 21 20 [Reporter]no spot (3L17)_PCR/8018 2 2 10 1 [Reporter]23s rRNA 1:4 (CtrlJ11)_PCR/2718 2 2 9 21 [Reporter]no spot (2N7)_PCR/2716 2 2 9 14 [Reporter]no spot (9B3)_PCR/2709 4 4 21 2 [Reporter]no spot (20P21)_PCR/8000 4 4 2 11 [Reporter]no spot (11L13)_PCR/7591 4 4 2 1 [Reporter]16s carry-over (CtrlL5)_PCR/7581 3 3 17 1 [Reporter]P5 (CtrlC18)_PCR/5392 4 4 1 1 [Reporter]Cy3 lights (CtrlH21)_PCR/7560 3 3 16 12 [Reporter]no spot (11G6)_PCR/5381 3 4 23 14 [Reporter]no spot (9K13)_PCR/7551 3 3 16 1 [Reporter]P4 (CtrlO14)_PCR/5370 3 2 8 20 [Reporter]no spot (3G7)_PCR/3197 3 4 23 11 [Reporter]no spot (12O9)_PCR/7548 3 4 23 1 [Reporter]Cy5 lights (CtrlO21)_PCR/7539 3 4 22 18 [Reporter]no spot (5G17)_PCR/7534 3 2 8 1 [Reporter]23s carry-over (CtrlC15)_PCR/3178 3 4 22 5 [Reporter]no spot (18C5)_PCR/7521 3 3 15 1 [Reporter]P3 (CtrlK14)_PCR/5348 3 4 22 2 [Reporter]50% DMSO (CtrlC1)_PCR/7518 3 4 22 1 [Reporter]Cy3 lights carry-over (CtrlK21)_PCR/7517 3 4 21 21 [Reporter]no spot (2O17)_PCR/7515 3 4 21 18 [Reporter]no spot (5C17)_PCR/7512 3 4 21 16 [Reporter]no spot (7K13)_PCR/7510 3 2 7 1 [Reporter]16s rRNA 1:256 (CtrlG3)_PCR/3156 3 3 14 1 [Reporter]P1 (CtrlG14)_PCR/5326 3 2 6 1 [Reporter]16s rRNA 1:64 (CtrlK3)_PCR/3134 3 3 13 1 [Reporter]23s rRNA 1:256 (CtrlO6)_PCR/5304 3 2 5 2 [Reporter]no spot (20O7)_PCR/3113 3 2 5 1 [Reporter]16s rRNA 1:16 (CtrlO3)_PCR/3112 3 2 4 20 [Reporter]no spot (3G3)_PCR/3109 2 3 17 2 [Reporter]no spot (20N18)_PCR/4889 2 3 17 1 [Reporter]P5 (CtrlB18)_PCR/4888 2 1 23 1 [Reporter]Cy5 lights (CtrlN24)_PCR/988 2 3 16 11 [Reporter]no spot (12B6)_PCR/4876 2 2 9 1 [Reporter]23s rRNA 1:1 (CtrlN11)_PCR/2696 2 1 22 5 [Reporter]no spot (18B8)_PCR/970 2 3 16 2 [Reporter]no spot (20J18)_PCR/4867 2 3 16 1 [Reporter]P4 (CtrlN14)_PCR/4866 2 1 22 2 [Reporter]50% DMSO (CtrlB4)_PCR/967 2 1 22 1 [Reporter]Cy3 lights carry-over (CtrlJ24)_PCR/966 2 2 8 1 [Reporter]23s carry-over (CtrlB15)_PCR/2674 2 2 7 20 [Reporter]no spot (3B7)_PCR/2671 2 3 15 1 [Reporter]P3 (CtrlJ14)_PCR/4844 2 1 21 1 [Reporter]P10 (CtrlB24)_PCR/944 2 3 14 11 [Reporter]no spot (12J2)_PCR/4832 2 2 7 1 [Reporter]16s rRNA 1:256 (CtrlF3)_PCR/2652 2 3 14 1 [Reporter]P1 (CtrlF14)_PCR/4822 2 2 6 16 [Reporter]no spot (6N23)_PCR/2645 2 1 20 1 [Reporter]P8 (CtrlN20)_PCR/922 2 2 6 1 [Reporter]16s rRNA 1:64 (CtrlJ3)_PCR/2630 2 3 13 1 [Reporter]23s rRNA 1:256 (CtrlN6)_PCR/4800 2 1 19 1 [Reporter]P7 (CtrlJ20)_PCR/900 2 2 5 1 [Reporter]16s rRNA 1:16 (CtrlN3)_PCR/2608 3 4 21 1 [Reporter]P10 (CtrlC21)_PCR/7495 3 4 20 1 [Reporter]P8 (CtrlO17)_PCR/7473 3 3 12 16 [Reporter]no spot (7G6)_PCR/5297 3 3 12 15 [Reporter]no spot (8C6)_PCR/5296 3 3 12 1 [Reporter]23s rRNA 1:64 (CtrlC10)_PCR/5282 3 4 19 1 [Reporter]P7 (CtrlK17)_PCR/7451 3 3 11 15 [Reporter]no spot (8O2)_PCR/5274 3 2 4 2 [Reporter]no spot (20K7)_PCR/3091 3 2 4 1 [Reporter]16s rRNA 1:4 (CtrlC7)_PCR/3090 3 3 11 1 [Reporter]23s rRNA 1:16 (CtrlG10)_PCR/5260 3 2 3 16 [Reporter]no spot (6C23)_PCR/3083 3 4 18 1 [Reporter]P6 (CtrlG17)_PCR/7429 3 4 17 20 [Reporter]no spot (3K13)_PCR/7426 3 2 3 1 [Reporter]16s rRNA 1:1 (CtrlG7)_PCR/3068 3 3 10 1 [Reporter]23s rRNA 1:4 (CtrlK10)_PCR/5238 3 2 2 11 [Reporter]no spot (11K15)_PCR/3056 3 4 17 1 [Reporter]P5 (CtrlC17)_PCR/7407 3 3 9 12 [Reporter]no spot (10K22)_PCR/5227 3 2 2 1 [Reporter]16s carry-over (CtrlK7)_PCR/3046 3 3 9 1 [Reporter]23s rRNA 1:1 (CtrlO10)_PCR/5216 3 3 8 20 [Reporter]no spot (3G6)_PCR/5213 3 3 8 18 [Reporter]no spot (5O2)_PCR/5211 3 2 1 10 [Reporter]no spot (12C15)_PCR/3033 3 2 1 4 [Reporter]no spot (18K7)_PCR/3027 3 2 1 1 [Reporter]Cy3 lights (CtrlG23)_PCR/3025 2 4 21 1 [Reporter]P10 (CtrlB21)_PCR/6991 2 2 23 1 [Reporter]Cy5 lights (CtrlN23)_PCR/3004 2 4 20 1 [Reporter]P8 (CtrlN17)_PCR/6969 2 3 12 1 [Reporter]23s rRNA 1:64 (CtrlB10)_PCR/4778 2 3 11 19 [Reporter]no spot (4N6)_PCR/4774 2 1 18 1 [Reporter]P6 (CtrlF20)_PCR/878 2 4 19 1 [Reporter]P7 (CtrlJ17)_PCR/6947 2 2 4 1 [Reporter]16s rRNA 1:4 (CtrlB7)_PCR/2586 2 2 3 20 [Reporter]no spot (3B3)_PCR/2583 2 3 11 1 [Reporter]23s rRNA 1:16 (CtrlF10)_PCR/4756 2 1 17 4 [Reporter]no spot (18J24)_PCR/859 2 1 17 1 [Reporter]P5 (CtrlB20)_PCR/856 2 4 18 1 [Reporter]P6 (CtrlF17)_PCR/6925 2 2 3 1 [Reporter]16s rRNA 1:1 (CtrlF7)_PCR/2564 2 4 17 15 [Reporter]no spot (8F9)_PCR/6917 2 4 17 9 [Reporter]no spot (14N1)_PCR/6911 2 3 10 1 [Reporter]23s rRNA 1:4 (CtrlJ10)_PCR/4734 2 1 16 1 [Reporter]P4 (CtrlN16)_PCR/834 2 4 17 1 [Reporter]P5 (CtrlB17)_PCR/6903 2 3 9 10 [Reporter]no spot (12B22)_PCR/4721 2 2 2 1 [Reporter]16s carry-over (CtrlJ7)_PCR/2542 2 3 9 8 [Reporter]no spot (14J18)_PCR/4719 2 3 9 1 [Reporter]23s rRNA 1:1 (CtrlN10)_PCR/4712 2 2 1 13 [Reporter]no spot (9N15)_PCR/2532 2 1 15 1 [Reporter]P3 (CtrlJ16)_PCR/812 2 3 8 20 [Reporter]no spot (3F6)_PCR/4709 2 2 1 10 [Reporter]no spot (12B15)_PCR/2529 2 2 1 1 [Reporter]Cy3 lights (CtrlF23)_PCR/2521 1 2 23 1 [Reporter]Cy5 lights (CtrlM23)_PCR/2500 3 4 16 15 [Reporter]no spot (8C9)_PCR/7399 3 4 16 1 [Reporter]P4 (CtrlO13)_PCR/7385 3 3 8 1 [Reporter]23s carry-over (CtrlC14)_PCR/5194 3 3 7 20 [Reporter]no spot (3C6)_PCR/5191 3 3 7 19 [Reporter]no spot (4O2)_PCR/5190 3 4 15 1 [Reporter]P3 (CtrlK13)_PCR/7363 3 3 7 1 [Reporter]16s rRNA 1:256 (CtrlG2)_PCR/5172 3 4 14 1 [Reporter]P1 (CtrlG13)_PCR/7341 3 4 13 21 [Reporter]no spot (2O9)_PCR/7339 3 3 6 10 [Reporter]no spot (12G18)_PCR/5159 3 3 6 1 [Reporter]16s rRNA 1:64 (CtrlK2)_PCR/5150 3 4 13 3 [Reporter]no spot (19G17)_PCR/7321 3 3 5 13 [Reporter]no spot (9O18)_PCR/5140 3 4 13 1 [Reporter]23s rRNA 1:256 (CtrlO5)_PCR/7319 3 3 5 12 [Reporter]no spot (10K18)_PCR/5139 3 3 5 1 [Reporter]16s rRNA 1:16 (CtrlO2)_PCR/5128 3 3 4 8 [Reporter]no spot (14G14)_PCR/5113 3 3 4 1 [Reporter]16s rRNA 1:4 (CtrlC6)_PCR/5106 2 4 16 1 [Reporter]P4 (CtrlN13)_PCR/6881 2 4 15 19 [Reporter]no spot (4N9)_PCR/6877 2 4 15 15 [Reporter]no spot (8N5)_PCR/6873 2 3 8 1 [Reporter]23s carry-over (CtrlB14)_PCR/4690 2 1 14 1 [Reporter]P1 (CtrlF16)_PCR/790 2 3 7 20 [Reporter]no spot (3B6)_PCR/4687 2 4 15 1 [Reporter]P3 (CtrlJ13)_PCR/6859 2 4 14 16 [Reporter]no spot (7N5)_PCR/6852 2 4 14 11 [Reporter]no spot (12J1)_PCR/6847 2 3 7 1 [Reporter]16s rRNA 1:256 (CtrlF2)_PCR/4668 2 1 13 1 [Reporter]23s rRNA 1:256 (CtrlN8)_PCR/768 1 2 22 5 [Reporter]no spot (18A7)_PCR/2482 2 4 14 1 [Reporter]P1 (CtrlF13)_PCR/6837 1 2 22 2 [Reporter]50% DMSO (CtrlA3)_PCR/2479 1 2 22 1 [Reporter]Cy3 lights carry-over (CtrlI23)_PCR/2478 2 1 12 13 [Reporter]no spot (10J4)_PCR/758 1 2 21 21 [Reporter]no spot (2M19)_PCR/2476 2 3 6 1 [Reporter]16s rRNA 1:64 (CtrlJ2)_PCR/4646 2 1 12 1 [Reporter]23s rRNA 1:64 (CtrlB12)_PCR/746 2 4 13 1 [Reporter]23s rRNA 1:256 (CtrlN5)_PCR/6815 2 3 5 15 [Reporter]no spot (7F22)_PCR/4638 2 3 5 13 [Reporter]no spot (9N18)_PCR/4636 1 2 21 1 [Reporter]P10 (CtrlA23)_PCR/2456 2 4 12 15 [Reporter]no spot (8B5)_PCR/6807 2 3 5 1 [Reporter]16s rRNA 1:16 (CtrlN2)_PCR/4624 2 1 11 1 [Reporter]23s rRNA 1:16 (CtrlF12)_PCR/724 1 2 20 1 [Reporter]P8 (CtrlM19)_PCR/2434 2 3 4 1 [Reporter]16s rRNA 1:4 (CtrlB6)_PCR/4602 1 2 19 11 [Reporter]no spot (12M7)_PCR/2422 2 1 10 1 [Reporter]23s rRNA 1:4 (CtrlJ12)_PCR/702 1 2 19 1 [Reporter]P7 (CtrlI19)_PCR/2412 3 4 12 2 [Reporter]no spot (20K13)_PCR/7298 4 1 23 5 [Reporter]no spot (18H8)_PCR/1999 3 4 12 1 [Reporter]23s rRNA 1:64 (CtrlC9)_PCR/7297 4 1 23 1 [Reporter]Cy5 lights (CtrlP24)_PCR/1996 4 1 22 14 [Reporter]no spot (9H16)_PCR/1987 4 1 22 9 [Reporter]no spot (14D12)_PCR/1982 3 4 11 1 [Reporter]23s rRNA 1:16 (CtrlG9)_PCR/7275 4 1 22 2 [Reporter]50% DMSO (CtrlD4)_PCR/1975 4 1 22 1 [Reporter]Cy3 lights carry-over (CtrlL24)_PCR/1974 4 1 21 22 [Reporter]no spot (1D24)_PCR/1973 3 4 10 18 [Reporter]no spot (5G5)_PCR/7270 3 4 10 11 [Reporter]no spot (11K21)_PCR/7263 3 4 10 9 [Reporter]no spot (13C21)_PCR/7261 3 3 3 1 [Reporter]16s rRNA 1:1 (CtrlG6)_PCR/5084 3 4 10 1 [Reporter]23s rRNA 1:4 (CtrlK9)_PCR/7253 4 1 21 1 [Reporter]P10 (CtrlD24)_PCR/1952 3 3 2 13 [Reporter]no spot (9C18)_PCR/5074 3 4 9 12 [Reporter]no spot (10K21)_PCR/7242 3 3 2 1 [Reporter]16s carry-over (CtrlK6)_PCR/5062 3 3 1 21 [Reporter]no spot (1O22)_PCR/5060 3 4 9 7 [Reporter]no spot (15G17)_PCR/7237 3 3 1 20 [Reporter]no spot (2K22)_PCR/5059 3 4 9 1 [Reporter]23s rRNA 1:1 (CtrlO9)_PCR/7231 4 1 20 1 [Reporter]P8 (CtrlP20)_PCR/1930 3 4 8 20 [Reporter]no spot (3G5)_PCR/7228 4 1 19 20 [Reporter]no spot (3D20)_PCR/1927 4 1 19 19 [Reporter]no spot (4P16)_PCR/1926 4 1 19 15 [Reporter]no spot (8P12)_PCR/1922 3 3 1 1 [Reporter]Cy3 lights (CtrlG22)_PCR/5041 3 4 8 2 [Reporter]no spot (20K9)_PCR/7210 3 4 8 1 [Reporter]23s carry-over (CtrlC13)_PCR/7209 4 1 19 1 [Reporter]P7 (CtrlL20)_PCR/1908 3 4 7 20 [Reporter]no spot (3C5)_PCR/7206 4 1 18 18 [Reporter]no spot (5H16)_PCR/1903 2 3 23 9 [Reporter]no spot (14F10)_PCR/5027 2 3 23 1 [Reporter]Cy5 lights (CtrlN22)_PCR/5020 2 4 12 5 [Reporter]no spot (17J17)_PCR/6797 2 4 12 1 [Reporter]23s rRNA 1:64 (CtrlB9)_PCR/6793 2 4 11 15 [Reporter]no spot (8N1)_PCR/6785 2 4 11 1 [Reporter]23s rRNA 1:16 (CtrlF9)_PCR/6771 2 3 3 1 [Reporter]16s rRNA 1:1 (CtrlF6)_PCR/4580 2 1 9 1 [Reporter]23s rRNA 1:1 (CtrlN12)_PCR/680 1 2 18 9 [Reporter]no spot (14A7)_PCR/2398 1 2 18 5 [Reporter]no spot (18A3)_PCR/2394 1 2 18 1 [Reporter]P6 (CtrlE19)_PCR/2390 2 1 8 14 [Reporter]no spot (8N24)_PCR/671 2 4 10 1 [Reporter]23s rRNA 1:4 (CtrlJ9)_PCR/6749 1 2 17 19 [Reporter]no spot (4E15)_PCR/2386 1 2 17 13 [Reporter]no spot (10M7)_PCR/2380 2 3 2 1 [Reporter]16s carry-over (CtrlJ6)_PCR/4558 2 1 8 1 [Reporter]23s carry-over (CtrlB16)_PCR/658 1 2 17 3 [Reporter]no spot (19E23)_PCR/2370 2 4 9 1 [Reporter]23s rRNA 1:1 (CtrlN9)_PCR/6727 2 4 8 20 [Reporter]no spot (3F5)_PCR/6724 1 2 17 1 [Reporter]P5 (CtrlA19)_PCR/2368 1 2 16 15 [Reporter]no spot (8A11)_PCR/2360 2 3 1 1 [Reporter]Cy3 lights (CtrlF22)_PCR/4537 2 1 7 4 [Reporter]no spot (18B16)_PCR/639 1 2 16 11 [Reporter]no spot (12A7)_PCR/2356 2 1 7 1 [Reporter]16s rRNA 1:256 (CtrlF4)_PCR/636 2 4 8 1 [Reporter]23s carry-over (CtrlB13)_PCR/6705 1 3 23 6 [Reporter]no spot (17I6)_PCR/4520 1 2 16 1 [Reporter]P4 (CtrlM15)_PCR/2346 1 2 15 17 [Reporter]no spot (6E11)_PCR/2340 1 3 23 5 [Reporter]no spot (18E6)_PCR/4519 1 3 23 1 [Reporter]Cy5 lights (CtrlM22)_PCR/4516 2 1 6 1 [Reporter]16s rRNA 1:64 (CtrlJ4)_PCR/614 1 2 15 7 [Reporter]no spot (15M23)_PCR/2330 1 2 15 5 [Reporter]no spot (17E23)_PCR/2328 1 2 15 1 [Reporter]P3 (CtrlI15)_PCR/2324 1 2 14 9 [Reporter]no spot (14A3)_PCR/2310 1 2 14 8 [Reporter]no spot (14M23)_PCR/2309 1 2 14 1 [Reporter]P1 (CtrlE15)_PCR/2302 3 4 7 8 [Reporter]no spot (14C17)_PCR/7194 3 4 7 1 [Reporter]16s rRNA 1:256 (CtrlG1)_PCR/7187 4 1 18 1 [Reporter]P6 (CtrlH20)_PCR/1886 3 4 6 19 [Reporter]no spot (4K1)_PCR/7183 4 1 17 16 [Reporter]no spot (7L12)_PCR/1879 3 4 6 1 [Reporter]16s rRNA 1:64 (CtrlK1)_PCR/7165 4 1 17 1 [Reporter]P5 (CtrlD20)_PCR/1864 4 1 16 17 [Reporter]no spot (6L12)_PCR/1858 4 1 16 7 [Reporter]no spot (16D4)_PCR/1848 3 4 5 2 [Reporter]no spot (20O5)_PCR/7144 4 1 16 4 [Reporter]no spot (18H24)_PCR/1845 3 4 5 1 [Reporter]16s rRNA 1:16 (CtrlO1)_PCR/7143 4 1 16 1 [Reporter]P4 (CtrlP16)_PCR/1842 3 4 4 12 [Reporter]no spot (10G17)_PCR/7132 4 1 15 11 [Reporter]no spot (12P4)_PCR/1830 3 4 4 1 [Reporter]16s rRNA 1:4 (CtrlC5)_PCR/7121 4 1 15 1 [Reporter]P3 (CtrlL16)_PCR/1820 4 1 14 22 [Reporter]no spot (1H16)_PCR/1819 3 4 3 3 [Reporter]no spot (19O5)_PCR/7101 2 4 7 1 [Reporter]16s rRNA 1:256 (CtrlF1)_PCR/6683 1 3 22 2 [Reporter]50% DMSO (CtrlA2)_PCR/4495 1 3 22 1 [Reporter]Cy3 lights carry-over (CtrlI22)_PCR/4494 2 1 5 1 [Reporter]16s rRNA 1:16 (CtrlN4)_PCR/592 2 4 6 7 [Reporter]no spot (15J13)_PCR/6667 2 4 6 1 [Reporter]16s rRNA 1:64 (CtrlJ1)_PCR/6661 2 1 4 20 [Reporter]no spot (3F4)_PCR/589 2 1 4 13 [Reporter]no spot (9J20)_PCR/582 2 4 5 12 [Reporter]no spot (10J17)_PCR/6650 1 2 13 20 [Reporter]no spot (3I11)_PCR/2299 1 3 21 1 [Reporter]P10 (CtrlA22)_PCR/4472 2 1 4 1 [Reporter]16s rRNA 1:4 (CtrlB8)_PCR/570 1 2 13 7 [Reporter]no spot (15E23)_PCR/2286 1 2 13 1 [Reporter]23s rRNA 1:256 (CtrlM7)_PCR/2280 2 4 5 1 [Reporter]16s rRNA 1:16 (CtrlN1)_PCR/6639 1 3 20 1 [Reporter]P8 (CtrlM18)_PCR/4450 2 1 3 6 [Reporter]no spot (16J12)_PCR/553 2 1 3 4 [Reporter]no spot (18B12)_PCR/551 2 1 3 1 [Reporter]16s rRNA 1:1 (CtrlF8)_PCR/548 2 4 4 1 [Reporter]16s rRNA 1:4 (CtrlB5)_PCR/6617 1 2 12 1 [Reporter]23s rRNA 1:64 (CtrlA11)_PCR/2258 1 3 19 1 [Reporter]P7 (CtrlI18)_PCR/4428 1 3 18 16 [Reporter]no spot (7M10)_PCR/4421 2 1 2 1 [Reporter]16s carry-over (CtrlJ8)_PCR/526 1 2 11 1 [Reporter]23s rRNA 1:16 (CtrlE11)_PCR/2236 1 3 18 1 [Reporter]P6 (CtrlE18)_PCR/4406 2 1 1 1 [Reporter]Cy3 lights (CtrlF24)_PCR/505 1 2 10 3 [Reporter]no spot (19I15)_PCR/2216 1 2 10 1 [Reporter]23s rRNA 1:4 (CtrlI11)_PCR/2214 1 2 9 19 [Reporter]no spot (4E7)_PCR/2210 4 2 22 5 [Reporter]no spot (18D7)_PCR/3994 4 2 22 2 [Reporter]50% DMSO (CtrlD3)_PCR/3991 4 2 22 1 [Reporter]Cy3 lights carry-over (CtrlL23)_PCR/3990 3 4 3 1 [Reporter]16s rRNA 1:1 (CtrlG5)_PCR/7099 4 1 14 1 [Reporter]P1 (CtrlH16)_PCR/1798 4 2 21 1 [Reporter]P10 (CtrlD23)_PCR/3968 3 4 2 10 [Reporter]no spot (12G13)_PCR/7086 4 1 13 9 [Reporter]no spot (13P24)_PCR/1784 4 1 13 7 [Reporter]no spot (15H24)_PCR/1782 4 2 20 14 [Reporter]no spot (9P11)_PCR/3959 3 4 2 1 [Reporter]16s carry-over (CtrlK5)_PCR/7077 4 1 13 1 [Reporter]23s rRNA 1:256 (CtrlP8)_PCR/1776 4 2 20 1 [Reporter]P8 (CtrlP19)_PCR/3946 4 2 19 11 [Reporter]no spot (12P7)_PCR/3934 3 4 1 1 [Reporter]Cy3 lights (CtrlG21)_PCR/7056 4 1 12 1 [Reporter]23s rRNA 1:64 (CtrlD12)_PCR/1754 4 1 11 20 [Reporter]no spot (3D12)_PCR/1751 4 2 19 1 [Reporter]P7 (CtrlL19)_PCR/3924 4 1 11 16 [Reporter]no spot (7D8)_PCR/1747 4 1 11 15 [Reporter]no spot (8P4)_PCR/1746 4 1 11 10 [Reporter]no spot (12L24)_PCR/1741 2 4 23 1 [Reporter]Cy5 lights (CtrlN21)_PCR/7035 4 1 11 1 [Reporter]23s rRNA 1:16 (CtrlH12)_PCR/1732 4 2 18 4 [Reporter]no spot (18P23)_PCR/3905 4 2 18 1 [Reporter]P6 (CtrlH19)_PCR/3902 2 4 22 2 [Reporter]50% DMSO (CtrlB1)_PCR/7014 2 4 22 1 [Reporter]Cy3 lights carry-over (CtrlJ21)_PCR/7013 4 1 10 1 [Reporter]23s rRNA 1:4 (CtrlL12)_PCR/1710 2 4 3 4 [Reporter]no spot (18B9)_PCR/6598 2 4 3 2 [Reporter]no spot (20F5)_PCR/6596 2 4 3 1 [Reporter]16s rRNA 1:1 (CtrlF5)_PCR/6595 2 4 2 12 [Reporter]no spot (10N13)_PCR/6584 2 4 2 1 [Reporter]16s carry-over (CtrlJ5)_PCR/6573 1 1 23 9 [Reporter]no spot (14E12)_PCR/491 1 3 17 1 [Reporter]P5 (CtrlA18)_PCR/4384 1 1 23 5 [Reporter]no spot (18E8)_PCR/487 1 1 23 1 [Reporter]Cy5 lights (CtrlM24)_PCR/484 2 4 1 1 [Reporter]Cy3 lights (CtrlF21)_PCR/6552 1 2 9 1 [Reporter]23s rRNA 1:1 (CtrlM11)_PCR/2192 1 3 16 1 [Reporter]P4 (CtrlM14)_PCR/4362 1 1 22 2 [Reporter]50% DMSO (CtrlA4)_PCR/463 1 1 22 1 [Reporter]Cy3 lights carry-over (CtrlI24)_PCR/462 1 4 23 1 [Reporter]Cy5 lights (CtrlM21)_PCR/6531 1 2 8 1 [Reporter]23s carry-over (CtrlA15)_PCR/2170 1 1 21 10 [Reporter]no spot (13A12)_PCR/449 1 3 15 1 [Reporter]P3 (CtrlI14)_PCR/4340 1 1 21 1 [Reporter]P10 (CtrlA24)_PCR/440 1 4 22 10 [Reporter]no spot (13E9)_PCR/6518 1 4 22 2 [Reporter]50% DMSO (CtrlA1)_PCR/6510 1 4 22 1 [Reporter]Cy3 lights carry-over (CtrlI21)_PCR/6509 1 3 14 12 [Reporter]no spot (11M2)_PCR/4329 1 2 7 1 [Reporter]16s rRNA 1:256 (CtrlE3)_PCR/2148 1 3 14 1 [Reporter]P1 (CtrlE14)_PCR/4318 1 1 20 2 [Reporter]no spot (20I24)_PCR/419 1 1 20 1 [Reporter]P8 (CtrlM20)_PCR/418 1 2 6 1 [Reporter]16s rRNA 1:64 (CtrlI3)_PCR/2126 1 2 5 22 [Reporter]no spot (1A7)_PCR/2125 1 1 19 5 [Reporter]no spot (18E4)_PCR/400 1 2 5 1 [Reporter]16s rRNA 1:16 (CtrlM3)_PCR/2104 4 2 17 10 [Reporter]no spot (13D7)_PCR/3889 4 2 17 1 [Reporter]P5 (CtrlD19)_PCR/3880 4 1 9 1 [Reporter]23s rRNA 1:1 (CtrlP12)_PCR/1688 4 2 16 1 [Reporter]P4 (CtrlP15)_PCR/3858 4 2 15 13 [Reporter]no spot (10H7)_PCR/3848 4 1 8 1 [Reporter]23s carry-over (CtrlD16)_PCR/1666 4 2 15 1 [Reporter]P3 (CtrlL15)_PCR/3836 4 1 7 10 [Reporter]no spot (12L20)_PCR/1653 4 2 14 8 [Reporter]no spot (14P23)_PCR/3821 4 1 7 2 [Reporter]no spot (20H12)_PCR/1645 4 1 7 1 [Reporter]16s rRNA 1:256 (CtrlH4)_PCR/1644 4 1 6 19 [Reporter]no spot (4L4)_PCR/1640 4 2 14 1 [Reporter]P1 (CtrlH15)_PCR/3814 4 1 6 1 [Reporter]16s rRNA 1:64 (CtrlL4)_PCR/1622 4 1 5 1 [Reporter]16s rRNA 1:16 (CtrlP4)_PCR/1600 1 1 5 12 [Reporter]no spot (10I20)_PCR/99 1 1 5 2 [Reporter]no spot (20M8)_PCR/89 1 1 5 1 [Reporter]16s rRNA 1:16 (CtrlM4)_PCR/88 1 4 21 10 [Reporter]no spot (13A9)_PCR/6496 1 4 21 1 [Reporter]P10 (CtrlA21)_PCR/6487 1 4 20 19 [Reporter]no spot (4A17)_PCR/6483 1 1 4 1 [Reporter]16s rRNA 1:4 (CtrlA8)_PCR/66 1 3 13 1 [Reporter]23s rRNA 1:256 (CtrlM6)_PCR/4296 1 1 3 7 [Reporter]no spot (15M12)_PCR/50 1 1 19 1 [Reporter]P7 (CtrlI20)_PCR/396 1 4 20 1 [Reporter]P8 (CtrlM17)_PCR/6465 1 3 12 16 [Reporter]no spot (7E6)_PCR/4289 1 1 3 1 [Reporter]16s rRNA 1:1 (CtrlE8)_PCR/44 1 4 19 13 [Reporter]no spot (10E9)_PCR/6455 1 3 12 1 [Reporter]23s rRNA 1:64 (CtrlA10)_PCR/4274 1 1 18 1 [Reporter]P6 (CtrlE20)_PCR/374 1 4 19 1 [Reporter]P7 (CtrlI17)_PCR/6443 1 1 2 1 [Reporter]16s carry-over (CtrlI8)_PCR/22 1 2 4 4 [Reporter]no spot (18E11)_PCR/2085 1 2 4 1 [Reporter]16s rRNA 1:4 (CtrlA7)_PCR/2082 1 3 11 1 [Reporter]23s rRNA 1:16 (CtrlE10)_PCR/4252 1 1 17 5 [Reporter]no spot (17M24)_PCR/356 1 2 3 14 [Reporter]no spot (8I19)_PCR/2073 1 1 17 1 [Reporter]P5 (CtrlA20)_PCR/352 1 4 18 8 [Reporter]no spot (15M1)_PCR/6428 1 4 18 1 [Reporter]P6 (CtrlE17)_PCR/6421 1 2 3 1 [Reporter]16s rRNA 1:1 (CtrlE7)_PCR/2060 1 4 17 16 [Reporter]no spot (7I9)_PCR/6414 1 3 10 4 [Reporter]no spot (18M14)_PCR/4233 1 3 10 1 [Reporter]23s rRNA 1:4 (CtrlI10)_PCR/4230 1 1 16 1 [Reporter]P4 (CtrlM16)_PCR/330 1 3 9 21 [Reporter]no spot (2M6)_PCR/4228 1 3 9 13 [Reporter]no spot (9M22)_PCR/4220 1 1 15 18 [Reporter]no spot (5I12)_PCR/325 1 2 2 1 [Reporter]16s carry-over (CtrlI7)_PCR/2038 1 2 1 22 [Reporter]no spot (1A3)_PCR/2037 1 3 9 1 [Reporter]23s rRNA 1:1 (CtrlM10)_PCR/4208 1 1 15 1 [Reporter]P3 (CtrlI16)_PCR/308 1 1 14 18 [Reporter]no spot (5E12)_PCR/303 1 2 1 3 [Reporter]no spot (19E7)_PCR/2018 1 2 1 1 [Reporter]Cy3 lights (CtrlE23)_PCR/2017 4 1 23 19 [Reporter]no spot (4P20)_PCR/2013 4 1 23 13 [Reporter]no spot (10H16)_PCR/2007 4 3 21 3 [Reporter]no spot (20H2)_PCR/5985 4 3 21 1 [Reporter]P10 (CtrlD22)_PCR/5983 4 3 20 16 [Reporter]no spot (7H14)_PCR/5976 4 3 20 14 [Reporter]no spot (9P10)_PCR/5974 4 3 20 11 [Reporter]no spot (12D10)_PCR/5971 4 2 13 5 [Reporter]no spot (17P19)_PCR/3796 4 2 13 1 [Reporter]23s rRNA 1:256 (CtrlP7)_PCR/3792 4 3 20 1 [Reporter]P8 (CtrlP18)_PCR/5961 4 2 12 18 [Reporter]no spot (5P7)_PCR/3787 4 2 12 11 [Reporter]no spot (12D3)_PCR/3780 4 3 19 18 [Reporter]no spot (5L14)_PCR/5956 4 1 4 20 [Reporter]no spot (3H4)_PCR/1597 4 2 12 1 [Reporter]23s rRNA 1:64 (CtrlD11)_PCR/3770 4 3 19 3 [Reporter]no spot (19P22)_PCR/5942 4 3 19 1 [Reporter]P7 (CtrlL18)_PCR/5940 4 2 11 15 [Reporter]no spot (8P3)_PCR/3762 4 1 4 2 [Reporter]no spot (20L8)_PCR/1579 4 1 4 1 [Reporter]16s rRNA 1:4 (CtrlD8)_PCR/1578 4 3 18 5 [Reporter]no spot (18D2)_PCR/5922 4 2 11 1 [Reporter]23s rRNA 1:16 (CtrlH11)_PCR/3748 4 3 18 1 [Reporter]P6 (CtrlH18)_PCR/5918 4 1 3 3 [Reporter]no spot (19P8)_PCR/1558 4 1 3 1 [Reporter]16s rRNA 1:1 (CtrlH8)_PCR/1556 4 2 10 1 [Reporter]23s rRNA 1:4 (CtrlL11)_PCR/3726 4 1 2 1 [Reporter]16s carry-over (CtrlL8)_PCR/1534 4 2 9 6 [Reporter]no spot (16D19)_PCR/3709 4 2 9 2 [Reporter]no spot (20P11)_PCR/3705 4 2 9 1 [Reporter]23s rRNA 1:1 (CtrlP11)_PCR/3704 4 2 8 20 [Reporter]no spot (3H7)_PCR/3701 4 1 1 1 [Reporter]Cy3 lights (CtrlH24)_PCR/1513 1 4 17 1 [Reporter]P5 (CtrlA17)_PCR/6399 1 4 16 1 [Reporter]P4 (CtrlM13)_PCR/6377 1 4 15 19 [Reporter]no spot (4M9)_PCR/6373 1 4 15 11 [Reporter]no spot (12M1)_PCR/6365 1 3 8 1 [Reporter]23s carry-over (CtrlA14)_PCR/4186 1 1 14 1 [Reporter]P1 (CtrlE16)_PCR/286 1 4 15 1 [Reporter]P3 (CtrlI13)_PCR/6355 1 3 7 14 [Reporter]no spot (8I22)_PCR/4177 1 3 7 1 [Reporter]16s rRNA 1:256 (CtrlE2)_PCR/4164 1 1 13 1 [Reporter]23s rRNA 1:256 (CtrlM8)_PCR/264 1 4 14 1 [Reporter]P1 (CtrlE13)_PCR/6333 1 4 13 15 [Reporter]no spot (8E5)_PCR/6325 1 3 6 1 [Reporter]16s rRNA 1:64 (CtrlI2)_PCR/4142 1 1 12 1 [Reporter]23s rRNA 1:64 (CtrlA12)_PCR/242 1 4 13 6 [Reporter]no spot (16A21)_PCR/6316 1 4 13 1 [Reporter]23s rRNA 1:256 (CtrlM5)_PCR/6311 1 1 11 19 [Reporter]no spot (4M8)_PCR/238 1 1 11 16 [Reporter]no spot (7A8)_PCR/235 1 4 12 20 [Reporter]no spot (3E9)_PCR/6308 1 3 5 10 [Reporter]no spot (12A18)_PCR/4129 1 3 5 1 [Reporter]16s rRNA 1:16 (CtrlM2)_PCR/4120 1 1 11 4 [Reporter]no spot (18A20)_PCR/223 1 1 11 1 [Reporter]23s rRNA 1:16 (CtrlE12)_PCR/220 4 3 17 4 [Reporter]no spot (18L22)_PCR/5899 4 3 17 1 [Reporter]P5 (CtrlD18)_PCR/5896 4 3 16 1 [Reporter]P4 (CtrlP14)_PCR/5874 4 2 8 7 [Reporter]no spot (15D19)_PCR/3688 4 2 8 1 [Reporter]23s carry-over (CtrlD15)_PCR/3682 4 3 15 1 [Reporter]P3 (CtrlL14)_PCR/5852 4 2 7 20 [Reporter]no spot (3D7)_PCR/3679 3 1 23 1 [Reporter]Cy5 lights (CtrlO24)_PCR/1492 4 3 14 19 [Reporter]no spot (4L10)_PCR/5848 4 3 14 13 [Reporter]no spot (10D6)_PCR/5842 4 2 7 1 [Reporter]16s rRNA 1:256 (CtrlH3)_PCR/3660 4 3 14 1 [Reporter]P1 (CtrlH14)_PCR/5830 4 2 6 14 [Reporter]no spot (8H23)_PCR/3651 3 1 22 2 [Reporter]50% DMSO (CtrlC4)_PCR/1471 3 1 22 1 [Reporter]Cy3 lights carry-over (CtrlK24)_PCR/1470 3 1 21 17 [Reporter]no spot (6O16)_PCR/1464 3 1 21 14 [Reporter]no spot (9C16)_PCR/1461 4 3 13 5 [Reporter]no spot (17P18)_PCR/5812 4 3 13 4 [Reporter]no spot (18L18)_PCR/5811 4 2 6 1 [Reporter]16s rRNA 1:64 (CtrlL3)_PCR/3638 4 3 13 1 [Reporter]23s rRNA 1:256 (CtrlP6)_PCR/5808 3 1 21 1 [Reporter]P10 (CtrlC24)_PCR/1448 4 2 5 1 [Reporter]16s rRNA 1:16 (CtrlP3)_PCR/3616 1 1 1 6 [Reporter]no spot (16A12)_PCR/5 1 1 1 1 [Reporter]Cy3 lights (CtrlE24)_PCR/1 3 1 20 1 [Reporter]P8 (CtrlO20)_PCR/1426