Array Design Name UMIST_S COELICOLOR_SC8_7337 Provider University of Manchester Institue of Science and Technology Comment[ArrayExpressAccession] A-MAXD-14 Comment[Description] GAPS II slide
Origin: Non-commercial Comment[SubmittedName] UMIST_S COELICOLOR_SC8_7337 Comment[Organism] Streptomyces coelicolor Technology Type spotted_ds_DNA_features Technology Type Term Accession Number #spotted_ds_DNA_features Technology Type Term Source REF The MGED Ontology Surface Type aminosilane Surface Type Term Accession Number #aminosilane Surface Type Term Source REF mo Term Source Name The MGED Ontology mo entrez Term Source File http://mged.sourceforge.net/ontologies/MGEDontology.php http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ncbi.nih.gov/Entrez/ Term Source Version Comment[AdditionalFile:txt] A-MAXD-14_comments.txt [main] Block Column Block Row Column Row Reporter Name Control Type Composite Element Name Composite Element Database Entry[entrez] Composite Element Comment 2 4 5 21 [Reporter]SCO5369 (2N1)_PCR/6659 [Gene]SCO5369/12132 NC_003888 2SC6G5.13, atpF, ATP synthase B chain (EC 3.6.1.34), len: 184aa; similar to many eg. SW:P00859 (ATPF_ECOLI) ATP synthase B chain from Escherichia coli (156 aa) fasta scores; opt: 246, z-score: 256.4, E(): 8.3e-07, 31.5% identity in 146 aa overlap. Almost identical to SW:P50013 (ATPF_STRLI) ATP synthase B chain from Streptomyces lividans (181 aa) fasta scores; opt: 1092, z-score: 1068.3, E(): 0, 97.3% identity in 184 aa overlap. Contains Pfam match to entry PF00430 ATP-synt_B, ATP synthase B/B', possible membrane-spanning hydrophobic regions and coiled-coil region 55-95aa. Contains possible hydrophobic membrane spanning region 2 4 5 20 [Reporter]SCO5878 (3J1)_PCR/6658 [Gene]SCO5878/12131 NC_003888 SC2E9.19, redX, polyketide synthase with two beta-ketoacyl synthase domains, len: 982 aa; similar to many eg. ERY3_SACER Q03133 erythronolide synthase, modules 5 and 6 (3170 aa), fasta scores; opt: 649 z-score: 586.3 E(): 1.9e-25, 29.2% identity in 1029 aa overlap. Contains 2 x Pfam match to entry ketoacyl-synt PF00109, Beta-ketoacyl synthases, scores 52.40 and 310.80 4 1 22 3 [Reporter]SCO1856 (20L4)_PCR/1976 [Gene]SCO1856/3607 NC_003888 SCI39.03, probable precorrin-6Y C5,15-methyltransferase, len: 410 aa; similar to SW:COBL_PSEDE (EMBL:M59301) Pseudomonas denitrificans precorrin-6Y C5,15-methyltransferase [decarboxylating] (EC 2.1.1.132) CobL, 413 aa; fasta scores: opt: 614 Z-score: 634.0 bits: 126.3 E(): 1e-27; 37.500% identity in 408 aa overlap. Contains Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases 4 1 21 21 [Reporter]SCO0506 (2P20)_PCR/1972 [Gene]SCO0506/3605 NC_003888 SCF6.02, nadE1, probable NH(3)-dependent NAD(+)synthetase, len: 276 aa. Highly similar to many e.g. Bacillus subtilis SW: NADE_BACSU (EMBL; M15811) NH(3)-dependent NAD(+) synthetase (EC 6.3.5.1) (spore outgrowth factor B) (sporulation protein OutB) (general stress protein 38) (Gsp38) (272 aa), fasta scores opt: 901 z-score: 1027.2 E():0 53.9% identity in 267 aa overlap. 2 4 5 19 [Reporter]SCO6159 (4F1)_PCR/6657 [Gene]SCO6159/12129 NC_003888 SC1A9.23, possible gntR family transcriptional regulator, len: 273 aa; similar to TR:O69996 (EMBL:AL022374) WhiH, sporulation transcription factor from Streptomyces coelicolor (295 aa) fasta scores; opt: 139, z-score: 267.9, E(): 1.2e-07, (29.8% identity in 292 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family, score 30.50, E-value 7.1e-08. 2 4 5 18 [Reporter]SCO7093 (5B1)_PCR/6656 [Gene]SCO7093/12127 NC_003888 SC3A4.19, possible transcriptional regulator, len: 932 aa. Highly similar to several including Streptomyces albus TR:Q53582(EMBL:U03114) transcriptional activator of the LipA promoter, LipR (890 aa), fasta scores opt: 714 z-score: 762.7 E(): 0 30.3% identity in 922 aa overlap and Streptomyces coelicolor TR:CAB52359(EMBL:AL109747) probable transcriptional regulator, SCJ21.13 (919 aa), fasta scores opt: 2398 z-score: 2564.6 E(): 0 48.0% identity in 911 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop) and a Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family with the putative helix-turn-helix motif situated between residues 883..904 (+3.20 SD). 2 4 5 17 [Reporter]SCO7053 (5N21)_PCR/6655 [Gene]SCO7053/12125 NC_003888 SC4G1.19c, hypothetical protein, len: 139 aa; similar to TR:O33283 (EMBL:AL008967) Mycobacterium tuberculosis hypothetical 16.6 kD protein, 149 aa; fasta scores: opt: 118 z-score: 161.0 E(): 0.17; 34.2% identity in 79 aa overlap 2 4 5 16 [Reporter]SCO7312 (6J21)_PCR/6654 [Gene]SCO7312/12123 NC_003888 SC5F8.22c, unknown, len: 77 aa. 2 4 5 15 [Reporter]SCO7422 (7F21)_PCR/6653 [Gene]SCO7422/12121 NC_003888 SC6D11.18c, cvnA10, possible sensor histidine-kinase, len: 543 aa. Similar to several from Streptomyces coelicolor including: TR:CAB61282 (EMBL:AL132991) putative sensor kinase, SCF55.12c (575 aa), fasta scores opt: 969 z-score: 843.9 E(): 0 42.9% identity in 501 aa overlap and TR:CAB59483 (EMBL:AL132648) putative integral membrane protein, SCI41.13c (541 aa), fasta scores opt: 630 z-score: 552.6 E(): 2.3e-23 32.9% identity in 559 aa overlap. Contains a Pfam match to entry PF00512 signal, Histidine kinase and a possible hydrophobic membrane spanning regions 2 4 5 14 [Reporter]SCO6886 (8B21)_PCR/6652 [Gene]SCO6886/12119 NC_003888 SC7F9.38, unknown, len: 211 aa. Highly similar to Streptomyces coelicolor TR:Q9X8A6 (EMBL:AL049645) hypothetical 21.3 KD protein, SCE2.11 (199 aa), fasta scores opt: 562 z-score: 585.7 E(): 3.3e-25 44.4% identity in 189 aa overlap 2 4 5 13 [Reporter]SCO6135 (9N17)_PCR/6651 [Gene]SCO6135/12117 NC_003888 SC9B2.22c, putative membrane protein, len: 224 aa; similar to SW:Y00U_MYCTU hypothetical protein from Mycobacterium tuberculosis (250 aa) fasta scores; opt: 125, z-score: 145.6, E(): 0.8, (26.4% identity in 208 aa overlap). Contains 4x5 residue perfect direct repeat, QTSVA, in the N-terminal region. Contains possible hydrophobic membrane spanning regions 2 4 5 11 [Reporter]SCO4359 (11F17)_PCR/6649 [Gene]SCO4359/12115 NC_003888 SCD19.14, probable ABC transport system ATP-binding protein, len: 336 aa; similar to SW:DRRA_STRPE (EMBL:M73758) Streptomyces peucetius daunorubicin resistance ATP-binding protein DrrA, 330 aa; fasta scores: opt: 912 z-score: 964.7 E(): 0; 49.5% identity in 327 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 2 4 5 10 [Reporter]SCO4275 (12B17)_PCR/6648 [Gene]SCO4275/12113 NC_003888 SCD95A.08, probable histidine autokinase, len: 428 aa; highly similar to TR:CAA74719 (EMBL:Y14336) Streptomyces reticuli histidine autokinase SenS, 398 aa; fasta scores: opt: 1703 z-score: 1743.6 E(): 0; 68.7% identity in 396 aa overlap. Contains possible hydrophobic membrane spanning regions in N-terminal domain 2 4 5 9 [Reporter]SCO0468 (13N13)_PCR/6647 [Gene]SCO0468/12111 NC_003888 SCF76.08c, unknown, len: 325 aa. The N-terminus is highly similar to several PIRIN proteins e.g. Alcaligenes eutrophus TR: AAD21775 (EMBL; J05278) PIRIN (187 aa), fasta scores opt: 446 z-score: 495.3 E(): 3.1e-20 49.7% identity in 149 aa overlap. Also similar to several hypothetical proteins e.g. Mycobacterium tuberculosis TR:O07425 (EMBL; Z97050) hypothetical 26.3 KD protein (244 aa), fasta scores opt: 269 z-score: 301.6 E(): 1.9e-09 32.7% identity in 217 aa overlap. 2 4 5 8 [Reporter]SCO0928 (14J13)_PCR/6646 [Gene]SCO0928/12109 NC_003888 SCM10.16c, hypothetical protein, len: 714 aa; similar to C-terminal region of TR:Q55576 (EMBL:D63999) Synechocystis sp. hypothetical 141.7 kD protein, 1244 aa; fasta scores: opt: 1179 z-score: 1354.7 E(): 0; 37.6% identity in 574 aa overlap and to S. coelicolor SC8D9.07 putative phosphodiesterase, 746 aa; fasta scores: opt: 817 z-score: 782.4 E(): 0; 41.8% identity in 452 aa overlap. Contains Pfam matches to entries PF00989 PAS, PAS domain, PF00785 PAC, PAC motif, PF00990 DUF9, Domain of unknown function and PF00563 DUF2, Domain of unknown function 2 2 4 5 7 [Reporter]SCO0494 (15F13)_PCR/6645 [Gene]SCO0494/12107 NC_003888 SCF34.13c, probable iron-siderophore binding lipoprotein, len: 350 aa; similar to TR:Q46023 (EMBL:U02617) Corynebacterium diphtheriae DtxR/iron-regulated lipoprotein precursor (355 aa), fasta scores; opt: 479 z-score: 518.8 E(): 1.5e-21, 34.4% identity in 358 aa overlap and SW:FATB_VIBAN (EMBL:J03529), FatB, Vibrio anguillarum plasmid pJM1 ferric anguibactin-binding protein precursor (322 aa) (26.2% identity in 305 aa overlap). Also similar to other iron-siderophore binding proteins. Contains an appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. Contains Pfam match to entry PF01497 Peripla_BP_2. Contains a probable coiled-coil from 189 to 219 (31 residues) (Max score: 1.558, probability 0.98) 2 4 5 6 [Reporter]SCO3135 (16B13)_PCR/6644 [Gene]SCO3135/12105 NC_003888 SCE66.14, probable acetyltransferase, len: 151 aa; similar to many especially SW:P19997 (BLS_STRSJ) blasticidin S-acetyltransferase (confers resistance to blasticidin S antibiotic) from Streptoverticillium sp. (strain JCM4673) (136 aa) fasta scores; opt: 465, z-score: 599.3, E(): 6.6e-26, 57.3% identity in 131 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 2 4 5 5 [Reporter]SCO3304 (17N9)_PCR/6643 [Gene]SCO3304/12103 NC_003888 SCE68.02, argS, probable arginyl-tRNA synthetase, len: 586 aa; similar to many e.g. SW:SYR_ECOLI (EMBL:X15320), argS, Escherichia coli arginyl-tRNA synthetase (577 aa), fasta scores; opt: 1415 z-score: 1562.9 E(): 0, 43.8% identity in 587 aa overlap. Contains Pfam match to entry PF00750 tRNA-synt_1d, tRNA synthetases class I (R) and PS00178 Aminoacyl-transfer RNA synthetases class-I signature 2 4 5 4 [Reporter]SCO0545 (18J9)_PCR/6642 [Gene]SCO0545/12101 NC_003888 SCF11.25, possible secreted protein, len: 843 aa; similar to TR:O87849 (EMBL:AL031013) Streptomyces coelicolor putative secreted protein, 602 aa; fasta scores: opt: 2859 z-score: 3026.4 E(): 0; 72.1% identity in 555 aa overlap. Contains possible N-terminal signal sequence 2 3 6 14 [Reporter]SCO5935 (8F22)_PCR/4659 [Gene]SCO5935/8499 NC_003888 SC7H1.05, putative membrane protein, len: 297 aa. Contains possible hydrophobic membrane spanning region 2 3 6 13 [Reporter]SCO6056 (9B22)_PCR/4658 [Gene]SCO6056/8497 NC_003888 SC9B1.03c, possible secreted protein, len: 133aa; contains possible N-terminal signal sequence. 2 3 6 12 [Reporter]SCO1962 (10N18)_PCR/4657 [Gene]SCO1962/8495 NC_003888 SCC54.22c, probable ionic transporter, len: 366 aa; similar to many egs. TR:O53910 (EMBL:AL022001) putative ionic transporter from Mycobacterium tuberculosis (360 aa) fasta scores; opt: 1466, z-score: 1650.6, E(): 0, (69.4% identity in 356 aa overlap) and SW:CHAA_ECOLI ChaA, calcium/proton antiporter from Escherichia coli (366 aa) fasta scores; opt: 668, z-score: 756.2, E(): 0, (34.7% identity in 352 aa overlap). Contains several probable membrane spanning hydrophobic regions. 2 3 6 11 [Reporter]SCO4530 (11J18)_PCR/4656 [Gene]SCO4530/8493 NC_003888 2SCD4.01, possible membrane protein (fragment), len: >147 aa. Contains possible hydrophobic membrane spanning region,SCD35.37, unknown, len: >77 aa 2 3 6 10 [Reporter]SCO4288 (12F18)_PCR/4655 [Gene]SCO4288/8491 NC_003888 SCD95A.21, possible phosphatase, len: 290 aa; similar to TR:O27788 (EMBL:AE000931) Methanobacterium thermoautotrophicum trehalose-6-phosphate phophatase related protein MTH1760, 264 aa; fasta scores: opt: 197 z-score: 223.2 E(): 5.7e-05; 28.3% identity in 258 aa overlap 2 3 6 9 [Reporter]SCO3879 (13B18)_PCR/4654 [Gene]SCO3879/8489 NC_003888 SCH18.16c, dnaA, chromosomal replication initiator protein (fragment), len: >606 aa; identical to C-terminal region previously sequenced SW:DNAA_STRCO (EMBL:M82836) Streptomyces coelicolor chromosomal replication initiator protein DnaA, 656 aa. Contains Pfam match to entry PF00308 bac_dnaA, Bacterial dnaA protein and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS01008 DnaA protein signature. Contains also a 5x degenerate repeat: (D/E)Y(D/E)GR,StH24.01c, dnaA, chromosomal replication initiator protein, partial CDS, len >81 aa; previously sequenced as SW:DNAA_STRCO (EMBL:M82836), DnaA 2 3 6 8 [Reporter]SCO3717 (14N14)_PCR/4653 [Gene]SCO3717/8487 NC_003888 SCH35.07, probable cation transport system component, len: 707 aa; similar to many e.g. SW:ATKB_ECOLI AtkB/KdpB potassium transporting ATPase A chain from Escherichia coli (682 aa) fasta scores; opt: 2748, z-score: 2854.0, E(): 0, (65.1% identity in 676 aa overlap). Contains Pfam match to entry PF00122 E1-E2_ATPase, E1-E2 ATPases and Prosite matches to PS00154 E1-E2 ATPases phosphorylation site and PS00018 EF-hand calcium-binding domain. Contains possible membrane spanning hydrophobic regions. 2 3 6 7 [Reporter]SCO1629 (15J14)_PCR/4652 [Gene]SCO1629/8485 NC_003888 SCI41.12c, cvnB9, unknown, len: 135 aa. Similar to TR:CAB41561 (EMBL:AL049727) Streptomyces coelicolor SC9B1.15C hypothetical 14.3 kD protein, 137 aa; fasta scores: opt: 266 z-score: 339.1 E(): 1.6e-11; 37.0% identity in 127 aa overlap and to TR:Q9AJP3 (EMBL:AB044803) Streptomyces girseus ORF2, 135 aa; fasta scores: opt: 559 Z-score: 671.5 E(): 9e-30; 62.687% identity in 134 aa overlap 2 3 6 6 [Reporter]SCO1715 (16F14)_PCR/4651 [Gene]SCO1715/8483 NC_003888 SCI11.04, hgd, probable homogentisate 1,2-dioxygenase, len: 441 aa; highly similar to eukaryotic homogentisate 1,2-dioxygenases e.g. SW:HGD_HUMAN (EMBL:U63008), hgd, Homo sapiens homogentisate 1,2-dioxygenase (445 aa), fasta scores; opt: 1551 z-score: 1809.0 E(): 0, 52.1% identity in 436 aa overlap 2 3 6 5 [Reporter]SCO3212 (17B14)_PCR/4650 [Gene]SCO3212/8481 NC_003888 SCE8.05c, trpD2, anthranilate phosphoribotransferase, len: 335 aa; similar to many e.g. SW:TRPD_AZOBR TrpD, anthranilate phosphoribotransferase from Azospirillum brasilense (355 aa) fasta scores; opt: 924, z-score: 1056.6, E(): 0, (49.5% identity in 315 aa overlap). Contains Pfam match to entry PF00591 Glycos_transf_3, glycosyl transferase family, score 223.70, E-value 2.8e-63. 2 3 6 4 [Reporter]SCO0898 (18N10)_PCR/4649 [Gene]SCO0898/8479 NC_003888 SCM1.31c, possible regulator, len: 660 aa. Similar to many other regulators including several from Streptomyces coelicolor e.g. SW:AFSR_STRCO (EMBL:D90155) regulatory protein AfsR (993 aa), fasta scores opt: 531 z-score: 560.0 E(): 7.5e-24 31.9% identity in 568 aa overlap and TR:Q9Z389(EMBL:AL035654) putative transcriptional regulator SCE8.10 (638 aa), fasta scores opt: 430 z-score: 456.1 E(): 4.6e-18 31.2% identity in 385 aa overlap. 1 4 17 21 [Reporter]SCO1000 (2M13)_PCR/6419 [Gene]SCO1000/11697 NC_003888 2SCG2.13c, integrase, len: 370 aa; N-terminal previously sequenced and annotated as TR:Q9X468 (EMBL:AF099015) Streptomyces coelicolor integrase Int, 222 aa. Contains Pfam match to entry PF00589 Phage_integrase, Phage integrase family 2 3 6 3 [Reporter]SCP1.10 (19J10)_PCR/4648 [Gene]SCP1.10/8477 NC_003888 SCP1.10, unknown, contains TTA encoded leucine at residue 33, possible target for bldA regulation. len: 164aa; 1 4 17 20 [Reporter]SCO6794 (3I13)_PCR/6418 [Gene]SCO6794/11695 NC_003888 SC1A2.03, cvnA7, possible membrane protein, len: 650 aa. Weakly similar to several including Streptomyces coelicolor TR:Q9X836(EMBL:AL049727) putative large secreted protein, SC9B1.16C (877 aa), fasta scores opt: 781 z-score: 616.3 E(): 6.4e-27 35.1% identity in 582 aa overlap and Streptomyces coelicolor TR:CAB61282(EMBL:AL132991) putative sensor kinase, SCF55.12C (575 aa), fasta scores opt: 426 z-score: 343.4 E(): 1e-11 28.9% identity in 508 aa overlap. Contains two possible membrane spanning hydrophobic domains towards the N-terminal and 4x VPAPV repeat between residues 485..504. 2 3 6 2 [Reporter]SCO4990 (20B10)_PCR/4647 [Gene]SCO4990/8475 NC_003888 2SCK36.13, conserved hypothetical protein, len: 136 aa; similar to SW:YABJ_BACSU (EMBL:D26185) Bacillus subtilis putative regulator of purine biosynthesis YabJ, 124 aa; fasta scores: opt: 239 Z-score: 301.4 bits: 61.4 E(): 3.5e-09; 36.641% identity in 131 aa overlap. Contains Pfam match to entry PF01042 UPF0076, YjgF family 1 4 17 19 [Reporter]SCO5476 (4E13)_PCR/6417 [Gene]SCO5476/11693 NC_003888 SC2A11.10, probable oligopeptide transport integral membrane protein, len: 335 aa; similar to mnay e.g. APPC_BACSU oligopeptide transport permease protein appC (303 aa), fasta scores; opt: 465 z-score: 1038.5 E(): 0, 41.4% identity in 309 aa overlap. Also similar to S. coelicolor TR:P72406 (EMBL:U68036) bldKA (335 aa), fasta scores; opt: 912 z-score: 1192.3 E(): 0, 42.1% identity in 330 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature and Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 47.00, E-value 4.1e-10 2 3 5 22 [Reporter]SCO5386 (1B6)_PCR/4645 [Gene]SCO5386/8473 NC_003888 2SC6G5.30, possible anti-sigma factor antagonist, len: 107aa; similar to many eg. SW:P95842 (RSBV_STAAU) anti-sigma B factor antagonist from Staphylococcus aureus (108 aa) fasta scores; opt: 164, z-score: 226.7, E(): 3.7e-05, 30.9% identity in 94 aa overlap. Contains Pfam match to entry PF01740 SpoIIAA, SpoIIAA family. 1 4 17 18 [Reporter]SCO6374 (5A13)_PCR/6416 [Gene]SCO6374/11691 NC_003888 SC4A2.10c, probable sugar transferase, len: 478; similar to many bacterial hypothetical proteins and to TR:O21943 (EMBL:AF021347) bactoprenol glucosyl transferase from bacteriophage SFII (309 aa), fasta scores; opt: 809 z-score: 925.5 E(): 0, 40.9% identity in 303 aa overlap. Contains a C-terminal extension with four additional transmembrane domains not present in similar proteins and Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases, score 46.50, E-value 6.1e-10 2 3 5 21 [Reporter]SCO3203 (2N2)_PCR/4644 [Gene]SCO3203/8471 NC_003888 SCE22.20, bar, phosphinothricin acetyltransferase, len: 171 aa; identical to previously sequenced SW:BAR_STRCO (EMBL:M62753) Streptomyces coelicolor phosphinothricin acetyltransferase (EC 2.3.1.-) Bar, 171 aa. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 2 3 5 20 [Reporter]SCO1440 (3J2)_PCR/4643 [Gene]SCO1440/8469 NC_003888 SC6D7A.03c, ribH, 6,7-dimethyl-8-ribityllumazine synthase, len: 161 aa; highly similar to SW:RISB_CORAM (EMBL:AB003693) Corynebacterium ammoniagenes 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.9) RibH, 163 aa; fasta scores: opt: 456 z-score: 563.8 E(): 7.2e-24; 46.8% identity in 158 aa overlap. Contains Pfam match to entry PF00885 DMRL_synthase, 6,7-dimethyl-8-ribityllumazine synthase 1 4 17 15 [Reporter]SCO5318 (8E9)_PCR/6413 [Gene]SCO5318/11687 NC_003888 SC6G9.15, polyketide beta-ketoacyl synthase alpha, len: 423 aa; whiE locus polyketide beta-ketoacyl synthase alpha previously sequenced from S. coelicolor as SW:KAS1_STRCO (EMBL:X55942). May form heterodimer with SW:KAS2_STRCO, SC6G9.16 beta-ketoacyl synthase beta. Similar to others from S.coelicolor e.g. TR:O54149 (EMBL:AL021409) probable 3-oxoacyl-[acyl-carrier-protein] synthase (407 aa) (39.1% identity in 419 aa overlap). Contains Pfam match to entry PF00109 ketoacyl-synt, Beta-ketoacyl synthase and PS00606 Beta-ketoacyl synthases active site 2 3 5 19 [Reporter]SCO6897 (4F2)_PCR/4642 [Gene]SCO6897/8467 NC_003888 SC1B2.03, unknown, len: 103 aa. 1 4 17 14 [Reporter]SCO5493 (9A9)_PCR/6412 [Gene]SCO5493/11685 NC_003888 SC8D9.05, unknown, len: 331aa; similar to others of undefined function eg. TR:O53262 (EMBL:AL021287) hypothetical protein from Mycobacterium tuberculosis (337 aa) opt: 774, z-score: 895.1, E(): 0, (43.4% identity in 334 aa overlap). 2 3 5 18 [Reporter]SCO6335 (5B2)_PCR/4641 [Gene]SCO6335/8465 NC_003888 SC3A7.03c, questionable ORF, len: 304 aa; this ORF is not predicted as coding by positional base preference 1 4 17 13 [Reporter]SCO2791 (10M5)_PCR/6411 [Gene]SCO2791/11683 NC_003888 SCC105.22c, unknown, len: 103 aa 2 3 5 17 [Reporter]SCO7061 (5N22)_PCR/4640 [Gene]SCO7061/8463 NC_003888 SC4G1.27c, probable marR-family transcriptional regulator, len: 178 aa; similar to SW:YXAD_BACSU (EMBL:AB005554) Bacillus subtilis hypothetical transcriptional regulator in AsnH-GntR intergenic region YxaD, 143 aa; fasta scores: opt: 242 z-score: 315.6 E(): 4.1e-10; 36.4% identity in 132 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family and possible helix-turn-helix motif at residues 65..86 (+3.24 SD) 1 4 17 12 [Reporter]SCO5284 (11I5)_PCR/6410 [Gene]SCO5284/11681 NC_003888 SCCB12.08c, hypothetical protein, len: 235 aa; similar to TR:Q9RVH7 (EMBL:AE001956) Deinococcus radiodurans hypothetical 24.5 kDa protein DR1052, 230 aa; fasta scores: opt: 403 z-score: 466.7 E(): 1.6e-18; 38.5% identity in 218 aa overlap and to SW:SPEE_ECOLI (EMBL:J02804) Escherichia coli spermidine synthase (EC 2.5.1.16) SpeE, 287 aa; fasta scores: opt: 173 z-score: 204.7 E(): 0.00063; 31.9% identity in 119 aa overlap 2 3 5 16 [Reporter]SCO7321 (6J22)_PCR/4639 [Gene]SCO7321/8461 NC_003888 SC5F8.31c, unknown, len: 354 aa. Similar to Deinococcus radiodurans TR:Q9RZT6(EMBL:AE001826) hypothetical TorS-related protein (351 aa), fasta scores opt: 393 z-score: 443.7 E(): 3e-17 34.7% identity in 354 aa overlap. 1 1 5 11 [Reporter]SCO4598 (11E20)_PCR/98 [Gene]SCO4598/181 NC_003888 SCD20.16c, possible two-component system sensor kinase, len: 407 aa; similar to TR:CAB81858 (EMBL:AL161691) Streptomyces coelicolor putative two-component sensor kinase SCD40A.13, 431 aa; fasta scores: opt: 659 z-score: 729.4 E(): 0; 37.0% identity in 427 aa overlap and to Streptomyces coelicolor putative two-component system sensor kinase SCD20.15c, 455 aa; fasta scores: opt: 1179 z-score: 1124.4 E(): 0; 57.2% identity in 442 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 5 9 [Reporter]SCO1823 (13N14)_PCR/4632 [Gene]SCO1823/8451 NC_003888 SCI8.08c, possible integral membrane protein, len: 124 aa. Similar to the C-terminus of Streptomyces coelicolor TR:O70002 (EMBL: AL022374) hypothetical 20.6 KD protein SC5B8.16 (192 aa), fasta scores opt: 268 z-score: 343.6 E(): 9.1e-12 36.9% identity in 111 aa overlap. Contains possible membrane spanning hydrophobic domains. 1 2 11 11 [Reporter]SCO4433 (11M23)_PCR/2246 [Gene]SCO4433/4099 NC_003888 SCD6.11c, possible integral membrane protein, len: 136 aa; similar to TR:O32937 (EMBL:Z98604) Mycobacterium leprae hypothetical 12.4 kD protein, 117 aa; fasta scores: opt: 308 z-score: 355.9 E(): 2.3e-12; 52.4% identity in 105 aa overlap. Contains possible hydrophobic membrane spanning regions 1 1 5 10 [Reporter]SCO4302 (12A20)_PCR/97 [Gene]SCO4302/179 NC_003888 SCD95A.35, possible secreted protein, len: 129 aa; N-terminal domain similar to TR:CAB69733 (EMBL:AL137166) Streptomyces coelicolor hypothetical 7.1 kD protein SCC121.15c, 68 aa; fasta scores: opt: 202 z-score: 262.4 E(): 3.7e-07; 52.5% identity in 59 aa overlap and C-terminal domain similar to TR:CAB69732 (EMBL:AL137166) Streptomyces coelicolor hypothetical 7.8 kD protein SCC121.14c, 73 aa; fasta scores: opt: 198 z-score: 257.2 E(): 7.3e-07; 51.6% identity in 64 aa overlap. Contains possible N-terminal region signal peptide sequence 1 2 11 7 [Reporter]SCO0599 (15M19)_PCR/2242 [Gene]SCO0599/4091 NC_003888 SCF55.23, probable regulator of sig8, 309 aa; identical to previously sequenced TR:CAB55832 (EMBL:AJ249580) Streptomyces coelicolor putative regulator of sig8, 309 aa and to TR:Q9ZBT2 (EMBL:AL034446) Streptomyces coelicolor putative regulatory protein SC1A9.20, 153 aa; fasta scores: opt: 167 z-score: 197.6 E(): 0.0012; 40.4% identity in 114 aa overlap 1 1 5 9 [Reporter]SCO3779 (13M16)_PCR/96 [Gene]SCO3779/177 NC_003888 SCH63.26, probable nucleoside hydrolase, len: 326 aa; similar to SW:IUNH_CRIFA (EMBL:U43371) Crithidia fasciculata inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1) IunH, 314 aa; fasta scores: opt: 634 z-score: 671.5 E(): 6.9e-30; 35.0% identity in 320 aa overlap. Contains Pfam match to entry PF01156 IU_nuc_hydro, Inosine-uridine preferring nucleoside hydrolase 1 1 5 8 [Reporter]SCO1785 (14I16)_PCR/95 [Gene]SCO1785/175 NC_003888 SCI51.25c, probable iron-siderophore uptake system ATP-binding component, len: 301 aa; similar to many ATP-binding components from binding-protein-dependent iron uptake systems using a range of siderophores e.g. SW:FEPC_ECOLI (EMBL:X57471), fepC, Escherichia coli ferric enterobactin transport ATP-binding protein (271 aa), fasta scores; opt: 1101 z-score: 1184.4 E(): 0, 61.5% identity in 260 aa overlap. Similar to other binding-protein-dependent permease subunits e.g. SC9B1.09 (EMBL:AL049727) S.coelicolor putative ABC transporter ATP-binding subunit (386 aa) (37.7% identity in 228 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 1 1 5 7 [Reporter]SCO3395 (15E16)_PCR/94 [Gene]SCO3395/173 NC_003888 SCE9.02c, putative secreted protein, len: 391 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Hydrophobic region at N-terminus and degenerate E(T/A)(P/R)EQ repeat in centre. Contains probable coiled-coil from 45 to 114 (70 residues) (Max score: 1.834, probability 1.00). Contains possible N-terminal region signal peptide sequence 1 1 5 6 [Reporter]SCO0031 (16A16)_PCR/93 [Gene]SCO0031/171 NC_003888 SCJ4.12c, unknown, len: 180 aa 2 3 5 8 [Reporter]SCO1687 (14J14)_PCR/4631 [Gene]SCO1687/8449 NC_003888 SCI30A.08, putative membrane protein, len: 130 aa; similar to SW:YIDH_ECOLI hypothetical protein from Escherichia coli (115 aa) fasta scores; opt: 291, z-score: 382.3, E(): 5.8e-14, (47.3% identity in 112 aa overlap). Contains possible hydrophobic membrane spanning regions 1 4 17 5 [Reporter]SCO0132 (17M21)_PCR/6403 [Gene]SCO0132/11667 NC_003888 SCJ21.13, probable transcriptional regulator, len: 919 aa; similar to many e.g. TR:AAD28307 (EMBL:U95170) Rhodococcus erythropolis ThcG (927 aa), fasta scores; opt: 1176 z-score: 1258.4 E(): 0, 34.2% identity in 933 aa overlap, and TR:AAA53487 (EMBL:U03114) Streptomyces albus LipR (890 aa), fasta scores; opt: 740 z-score: 791.4 E(): 0, 31.1% identity in 952 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop), Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, and probable helix-turn-helix motif at aa 871-892 (Score 1389, +3.92 SD) 2 3 5 7 [Reporter]SCO0451 (15F14)_PCR/4630 [Gene]SCO0451/8447 NC_003888 SCF51A.29c, unknown, len: 284 aa. Similar to the C-terminus of many hypothetical proteins e.g. Mycobacterium tuberculosis SW:Y07S_MYCTU (EMBL; Z75555) hypothetical 69.5 KD protein CY02B10.28C (653 aa), fasta scores opt: 390 z-score: 451.1 E(): 9.1e-18 33.5% identity in 263 aa overlap. In addition to Bacillus subtilis SW:RSBU_BACSU (EMBL; L35574) sigma factor SibG regulation protein RsbU (335 aa), fasta scores opt: 206 z-score: 243.6 E(): 3.3e-06 24.3% identity in 185 aa overlap. 1 4 17 4 [Reporter]SCO4059 (18I21)_PCR/6402 [Gene]SCO4059/11665 NC_003888 2SCD60.25, possible transcriptional regulator, len: 111 aa; similar to TR:O86818 (EMBL:AL031031) Streptomyces coelicolor putative transcriptional regulator SC7C7.13, 124 aa; fasta scores: opt: 200 z-score: 263.0 E(): 4.4e-07; 43.3% identity in 97 aa overlap. Contains Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family and possible helix-turn-helix motif at residues 30..51 (+4.25 SD) 2 3 5 6 [Reporter]SCO3888 (16B14)_PCR/4629 [Gene]SCO3888/8445 NC_003888 StH24.10, conserved hypothetical protein, len: 205 aa; previously sequenced as TR:O07326 (EMBL:AF031590), unknown function, similar to TR:O53594 (EMBL:AL021426) Mycobacterium tuberculosis hypothetical protein (244 aa), fasta scores; opt: 392 z-score: 473.7 E(): 4.5e-19, 40.2% identity in 234 aa overlap and TR:Q50200 (EMBL:L39923) hypothetical protein (259 aa) (39.6% identity in 240 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family, score 17.80, E-value 0.018 1 4 17 3 [Reporter]SCP1.13c (19E21)_PCR/6401 [Gene]SCP1.13c/11663 NC_003888 SCP1.13c, conserved hypothetical protein, len: 538aa; C-terminal similar to TR:Q9KXL1 (EMBL:AL357524) hypothetical protein from Streptomyces coelicolor (282 aa) fasta scores; opt: 682, z-score: 810.6, E(): 0, 51.1% identity in 235 aa overlap. Contains TTA encoded leucine at residue 154, possible target for bldA regulation. 2 3 5 5 [Reporter]SCO3862 (17N10)_PCR/4628 [Gene]SCO3862/8443 NC_003888 SCH69.32c, putative membrane protein, len: 93 aa. Contains possible hydrophobic membrane spanning region 1 4 17 2 [Reporter]SCO5080 (20M17)_PCR/6400 [Gene]SCO5080/11661 NC_003888 SCBAC28G1.06, actVA5, possible hydrolase, len: 381 aa; identical to previously sequenced TR:Q53907 (EMBL:X58833) Streptomyces coelicolor 6 ActVA region genes of the actinorhodin biosynthetic gene cluster, ActVA5, 381 aa and similar to TR:Q9F0J3 (EMBL:AF218066) Streptomyces arenae hydrolase NcnH, 405 aa; fasta scores: opt: 1391 Z-score: 1521.5 bits: 290.3 E(): 3.9e-77; 56.522% identity in 368 aa overlap 2 3 5 4 [Reporter]SCO1707 (18J10)_PCR/4627 [Gene]SCO1707/8441 NC_003888 SCI30A.28c, probable ABC sugar transporter, ATP-binding subunit, len: 445aa; similar to many eg. TR:AAD24578 (EMBL:AF126010) trehalose/maltose transport ATP-hydrolysing subunit from hyperthermophilic archaeon Thermococcus litoralis (372 aa) fasta scores; opt: 975, z-score: 1068.2, E(): 0, (50.1% identity in 335 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite matches to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 1 2 11 6 [Reporter]SCO1797 (16I19)_PCR/2241 [Gene]SCO1797/4089 NC_003888 SCI5.05c, possible integral membrane protein, len: 143aa; similar to TR:P71767 (EMBL:Z79701) hypothetical protein from Mycobacterium tuberculosis (144 aa) fasta scores; opt: 344, z-score: 409.3, E(): 1.8e-15, (44.8% identity in 134 aa overlap). Contains possible membrane spanning hydrophobic regions. 1 1 5 5 [Reporter]SCO4964 (17M12)_PCR/92 [Gene]SCO4964/169 NC_003888 2SCK31.24, probable integral membrane transport protein, len: 285 aa; similar to TR:AAG32069 (EMBL:AF317791) Streptomyces viridochromogenes hydrophobic transmembrane protein AviABCII, 265 aa; fasta scores: opt: 677 z-score: 786.2 E(): 0; 39.7% identity in 262 aa overlap. Contains possible hydrophobic membrane spanning regions 1 2 11 2 [Reporter]SCO5096 (20E15)_PCR/2237 [Gene]SCO5096/4081 NC_003888 SCBAC28G1.22c, LysR-family transcriptional regulatory protein, len: 309 aa; identical to previously sequenced TR:Q9X9U9 (EMBL:Y18817) Streptomyces coelicolor A3(2) transcriptional regulator of LysR family, 307 aa. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Also contains a possible helix-turn-helix motif at residues 22..43 (+5.22 SD) 1 1 5 4 [Reporter]SCO3808 (18I12)_PCR/91 [Gene]SCO3808/167 NC_003888 SCGD3.09, hypothetical protein, len: 274 aa; unknown function, similar to TR:O05777 (EMBL:Z95150) Mycobacterium tuberculosis hypothetical protein (283 aa), fasta scores; opt: 384 z-score: 466.0 E(): 1.3e-18, 32.7% identity in 269 aa overlap 1 1 5 3 [Reporter]SCP1.283 (19E12)_PCR/90 [Gene]SCP1.283/165 NC_003888 SCP1.283, doubtful CDS, unknown, len: 54aa; 1 1 4 22 [Reporter]SCO4199 (1M4)_PCR/87 [Gene]SCO4199/163 NC_003888 2SCD46.13, unknown, len: 213aa; contains mostly hydrophilic residues. 1 1 4 21 [Reporter]SCO5960 (2I4)_PCR/86 [Gene]SCO5960/161 NC_003888 SC7H1.30c, cbiN, probable cobalt transport protein, len: 118 aa; similar to many eg. CBIN_SALTY Q05595 cobalt transport protein cbin (93 aa), fasta scores; opt: 283 z-score: 433.7 E(): 5.8e-17, 47.3% identity in 93 aa overlap. Contains possible N-terminal signal sequence 2 3 4 22 [Reporter]SCO4546 (1N2)_PCR/4623 [Gene]SCO4546/8435 NC_003888 2SCD4.17, possible lipoprotein, len: 216 aa; similar to TR:CAB92614 (EMBL:AL356813) Streptomyces coelicolor putative lipoprotein SCK15.23, 221 aa; fasta scores: opt: 525 z-score: 595.2 E(): 1.1e-25; 39.4% identity in 213 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal signal peptide sequence 1 4 16 19 [Reporter]SCO5468 (4A13)_PCR/6395 [Gene]SCO5468/11653 NC_003888 SC2A11.02c, probable transmembrane transport protein, len: 472 aa; similar to many e.g. YHJE_ECOLI hypothetical metabolite transport protein (440 aa), fasta scores; opt: 772 z-score: 1638.5 E(): 0, 56.7% identity in 441 aa overlap and SHIA_ECOLI shikimate transporter (438 aa), fasta scores; opt: 553 z-score: 980.0 E(): 0, 34.6% identity in 437 aa overlap. Contains PS00216 Sugar transport proteins signature 1 and Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporters, score 183.10, E-value 4.4e-51 2 3 4 21 [Reporter]SCO5260 (2J2)_PCR/4622 [Gene]SCO5260/8433 NC_003888 2SC7G11.22c, atrA, secreted protein, len: 321 aa; identical to previously sequenced TR:Q9RGW8 (EMBL:AF104994) Streptomyces coelicolor A3(2) periplasmic binding protein AtrA, 321 aa. Contains Pfam match to entry PF00497 SBP_bac_3, Bacterial extracellular solute-binding proteins, family 3 and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 1 4 16 18 [Reporter]SCO2085 (5M9)_PCR/6394 [Gene]SCO2085/11651 NC_003888 SC4A10.18c, ftsW, probable cell division protein, len; 456 aa; previously sequenced as TR:Q9ZBA6 (EMBL:U10879), ftsW, Streptomyces coelicolor ftsW protein (456 aa) and identical to that sequence. Similar to many members of the ftsW/rodA/spoVE family e.g. SW:SP5E_BACSU (EMBL:X51419), spoVE, Bacillus subtilis stage V sporulation protein (366 aa), fasta scores; opt: 745 z-score: 788.4 E(): 0, 36.9% identity in 350 aa overlap. Shows similarity to SC6G9.31 (EMBL:AL079356) S.coelicolor probable integral membrane cell-cycle protein (446 aa) (30.0% identity in 416 aa overlap) and SCH69.16 (EMBL:AL079308) S.coelicolor probable FtsW/RodA/SpoVE family cell cycle protein (479 aa) (28.6% identity in 385 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF01098 FTSW_RODA_SPOVE, Cell cycle protein and PS00428 Cell cycle proteins ftsW/rodA/spoVE signature 2 3 4 20 [Reporter]SCO2606 (3F2)_PCR/4621 [Gene]SCO2606/8431 NC_003888 SCC88.17c, orfsyn, hypothetical protein, len: 661 aa; N-terminal region identical to previously sequenced TR:O33624 (EMBL:Y14206) Streptomyces coelicolor ORFSYN (fragment) and similar to TR:P73260 (EMBL:D90905) Synechocystis sp. hypothetical 97.4 kD protein 865 aa; fasta scores: opt: 1055 z-score: 1198.6 E(): 0; 41.2% identity in 624 aa overlap. Contains match to Prosite entry PS00133 Zinc carboxypeptidases, zinc-binding region 2 signature 1 2 10 22 [Reporter]SCO2799 (1E11)_PCR/2235 [Gene]SCO2799/4079 NC_003888 2SCC13.07c, possible secreted sugar hydrolase, len: 489 aa; similar to the C-terminal region of TR:Q9UWR7 (EMBL:AB024740) Pyrococcus kodakaraensis chitinase PK-ChiA, 1215 aa; fasta scores: opt: 671 z-score: 561.2 E(): 9.4e-24; 31.6% identity in 471 aa overlap. Contains Pfam match to entry PF00553 CBD_2, Cellulose binding domain and 3x degenerate repeat: PAPT(P/G)TP. Also possible N-terminal region signal peptide sequence 1 1 4 20 [Reporter]SCO2643 (3E4)_PCR/85 [Gene]SCO2643/159 NC_003888 SC8E4A.13, pepN, aminopeptidase N, len: 857 aa; identical to SW:AMPN_STRLI (EMBL:L23172) Streptomyces lividans aminopeptidase N (EC 3.4.11.2) PepN, 857 aa and similar to SW:AMPN_LACLA (EMBL:D38040) Lactococcus lactis aminopeptidase N (EC 3.4.11.2) PepN, 848 aa; fasta scores: opt: 738 z-score: 846.7 E(): 0; 33.4% identity in 440 aa overlap and to S. coelicolor SC8E4A.05, 833 aa; fasta scores: opt: 2998 z-score: 2943.4 E(): 0; 55.8% identity in 842 aa overlap. Contains Pfam match to entry PF01433 Peptidase_M1, Peptidase family M1 and match ot Prosite entry PS00142 Neutral zinc metallopeptidases, zinc-binding region signature 1 2 10 18 [Reporter]SCO5732 (5E7)_PCR/2231 [Gene]SCO5732/4071 NC_003888 SC3C3.18c, unknown, len: 517 aa; C-terminus is Gly-Pro-rich, and is predicted to be non-coding by FramePlot 1 1 4 19 [Reporter]SCO6908 (4A4)_PCR/84 [Gene]SCO6908/157 NC_003888 SC1B2.14c, unknown, len: 434 aa. 1 1 4 18 [Reporter]SCO7088 (4M24)_PCR/83 [Gene]SCO7088/155 NC_003888 SC3A4.14c, two-component system response regulator, len: 225 aa. Similar to Escherichia coli SW:NARL_ECOLI(EMBL:X13360) nitrate/nitrite response regulator protein, NarL (216 aa), fasta scores opt: 375 z-score:0.0 E(): 0.0 33.79% identity in 219 aa overlap. Also highly similar to multiple Streptomyces coelicolor putative two-component response regulators e.g. TR:CAB59718(EMBL:AL132707) SCF51.20C (219 aa), fasta scores opt: 933 z-score: 1109.1 E():0 73.3% identity in 217 aa overlap. Contains a Prosite hits to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00622 Bacterial regulatory proteins, luxR family signature. Also contains Pfam matches to entries PF00196 GerE, Bacterial regulatory proteins, luxR family and PF00072 response_reg, Response regulator receiver domain with the putative helix-turn-helix motif situated between residues 174..195 (+3.75 SD). 1 1 4 17 [Reporter]SCO7073 (5I24)_PCR/82 [Gene]SCO7073/153 NC_003888 SC4G1.39c, hypothetical protein, len: 330 aa; similar to SW:YCGT_ECOLI (EMBL:AE000218) Escherichia coli hypothetical 39.5 kDa protein in TreA-Pth intergenic region YcgT, 366 aa; fasta scores: opt: 876 z-score: 955.5 E(): 0; 46.2% identity in 355 aa overlap, to N-terminal region of SW:DAK1_YEAST (EMBL:Z38114) Saccharomyces cerevisiae dihydroxyacetone kinase 1 (EC 2.7.1.29) Dak1, 584 aa; fasta scores: opt: 516 z-score: 562.8 E(): 7e-24; 34.0% identity in 312 aa overlap and to TR:AAK84068 (EMBL:AF297121) Selenomonas ruminantium subsp. ruminantium dihydroxyacetone kinase DhaK1, 329 aa; fasta scores: opt: 1017 Z-score: 1086.8 E(): 6.7e-53; 49.848% identity in 329 aa overlap 1 1 4 16 [Reporter]SCO6734 (6E24)_PCR/81 [Gene]SCO6734/151 NC_003888 SC5F2A.17, probable amino acid permease, len: 489aa; similar to many especially SW:ANSP_SALTY AnsP, L-asparagine permease from Salmonella enterica (497 aa) fasta scores; opt: 1886, z-score: 2042.9, E(): 0, (58.6% identity in 476 aa overlap). Contains Pfam match to entry PF00324 aa_permeases, Amino acid permease and Prosite match to PS00218 Amino acid permeases signature. Also contains several probable membrane spanning hydrophobic regions. 1 1 4 15 [Reporter]SCO1436 (7A24)_PCR/80 [Gene]SCO1436/149 NC_003888 SC6D7.03, possible secreted protein, len: 443 aa. Highly similar to many proteins of unknown function including: Mycobacterium tuberculosis SW:Y0D3_MYCTU(EMBL:Z78020) hypothetical 36.7 KD protein (345 aa), fasta scores opt: 1088 z-score: 1211.2 E():0 44.1% identity in 447 aa overlap and Streptomyces coelicolor TR:CAB53286(EMBL:AL109972) putative integral membrane protein SCJ9A.25C (445 aa), fast scores opt: 1023 z-score: 1139.2 E():0 42.4% identity in 429 aa overlap. Also similar to the adjoining CDS SC6D7.04 (347 aa), fasta scores opt: 651 z-score: 585.4 E(): 3e-27 35.9% identity in 343 aa overlap. Contains a Pfam matches to entries PF01595 DUF21, Domain of unknown function and 2x Pfam match to entry PF00571 CBS, CBS domain. Also contains possible hydrophobic membrane spanning regions 1 2 10 13 [Reporter]SCO6490 (10A3)_PCR/2226 [Gene]SCO6490/4061 NC_003888 SC9C7.26, possible acetyltransferase, len: 199 aa; similar to SW:RIMJ_ECOLI ribosomal-protein-alanine acetyltransferase from Escherichia coli (194 aa) fasta scores; opt: 248, z-score: 351.1, E(): 2.9e-12, (38.7% identity in 111 aa overlap). 1 1 4 14 [Reporter]SCO7180 (8M20)_PCR/79 [Gene]SCO7180/147 NC_003888 SC8A11.08c, hypothetical protein, len: 117 aa; similar to TR:AAF70105 (EMBL:AF257324) Streptomyces galilaeus polyketide oxygenase AknX, 122 aa; fasta scores: opt: 151 z-score: 213.3 E(): 0.00021; 34.3% identity in 105 aa overlap 1 1 4 13 [Reporter]SCO6452 (9I20)_PCR/78 [Gene]SCO6452/145 NC_003888 SC9B5.19, probable transport permease protein, len: 334aa; second of a five gene operon probably involved in peptide uptake, similar to many transport permeases eg. SW:DPPB_ECOLI DppB, dipeptide transport system permease protein from Escherichia coli (339 aa) fasta scores: opt: 362, z-score: 742.8, E(): 0, (32.9% identity in 340 aa overlap). 1 1 4 12 [Reporter]SCO2305 (10E20)_PCR/77 [Gene]SCO2305/143 NC_003888 SCC30.13, probable ABC transporter ATP-binding subunit, len: 308 aa; similar to TR:AAF12291 (EMBL:AE001862) Deinococcus radiodurans ABC transporter, ATP-binding protein, putative, DRA0007, 301 aa; fasta scores: opt: 753 z-score: 842.3 E(): 0; 47.9% identity in 288 aa overlap and to SW:NODI_RHILO (EMBL:X55705) Rhizobium loti nodulation ATP-binding protein I, NodI, 339 aa; fasta scores: opt: 576 z-score: 645.7 E(): 1.6e-28; 37.3% identity in 303 aa overlap. Also similar to various Streptomyces coelicolor ABC transporter ATP-binding proteins, e.g. TR:CAB56355 (EMBL:AL118514) S. coelicolor ABC transport protein, ATP-binding subunit SCD25.11c, 326 aa; fasta scores: opt: 606 z-score: 418.1 E(): 8.6e-18; 41.6% identity in 296 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 1 4 11 [Reporter]SCO4592 (11A20)_PCR/76 [Gene]SCO4592/141 NC_003888 SCD20.10c, possible integral membrane protein, len: 362 aa; similar to SW:RARD_ECOLI (EMBL:L02122) Escherichia coli RarD protein, 296 aa; fasta scores: opt: 792 z-score: 820.5 E(): 0; 42.3% identity in 291 aa overlap. Contains Pfam match to entry PF00892 DUF6, Integral membrane protein DUF6 and possible hydrophobic membrane spanning regions 2 3 4 11 [Reporter]SCO4363 (11B18)_PCR/4612 [Gene]SCO4363/8413 NC_003888 SCD19.18, probable two component system response regulator, len: 226 aa; similar to TR:O70001 (EMBL:AL022374) Streptomyces coelicolor putative two-component regulator SC5B8.15, 229 aa; fasta scores: opt: 691 z-score: 751.7 E(): 0; 54.4% identity in 215 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Also contains possible helix-turn-helix motif at residues 181..202 (+3.68 SD) 1 4 16 8 [Reporter]SCO0173 (15E1)_PCR/6384 [Gene]SCO0173/11631 NC_003888 SCJ1.22c, unkown, len: 260 aa. 2 3 4 10 [Reporter]SCO3290 (12N14)_PCR/4611 [Gene]SCO3290/8411 NC_003888 SCE15.07, unknown, len: 249aa; 1 2 10 12 [Reporter]SCO2684 (10M23)_PCR/2225 [Gene]SCO2684/4059 NC_003888 SCC61A.05c, possible ATP-binding membrane protein, len: 826aa; no significant database similarities, N-terminal region rich in glycine/arginine/asparagine, C-terminal contains possible membrane-spanning hydrophobic region. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). Note possible alternative downstream translational start sites. 1 1 4 10 [Reporter]SCO2903 (12M16)_PCR/75 [Gene]SCO2903/139 NC_003888 SCE19A.03c, possible secreted protein, len: 134 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains probable N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site, so may be a lipoprotein 1 2 10 8 [Reporter]SCO0845 (14M19)_PCR/2221 [Gene]SCO0845/4051 NC_003888 SCF43A.35c, unknown, len: 88 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. The N-terminus is rich in basic amino acids, while the remainder is rich in Ser/Thr 1 1 4 9 [Reporter]SCO1616 (13I16)_PCR/74 [Gene]SCO1616/137 NC_003888 SCI35.38c, probable transcriptional regulator, len: 325 aa; similar to many members of the lysR family CBBR_RHORU RUBISCO operon transcriptional regulator (298 aa), fasta scores; opt: 394 z-score: 410.2 E(): 1.3e-15, 30.2% identity in 295 aa overlap. Contains PS00044 Bacterial regulatory proteins, lysR family signature, possible helix-turn-helix motif at aa 53-74 (Score 1391, +3.92 SD), and Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix proteins, lysR family, score 129.30, E-value 7e-35 1 1 4 8 [Reporter]SCO3850 (14E16)_PCR/73 [Gene]SCO3850/135 NC_003888 SCH69.20c, possible integral membrane protein, len: 352 aa; contains possible membrane spanning hydrophobic region. Similar to neighbouring gene SCH69.19c fasta scores; opt: 651, z-score: 547.1, E(): 2.3e-25, (45.9% identity in 218 aa overlap) 1 1 4 7 [Reporter]SCO3626 (15A16)_PCR/72 [Gene]SCO3626/133 NC_003888 SCH10.04c, probable RNA polymerase sigma factor, len: 294 aa; similar to many e.g. TR:O05735 (EMBL:U87308) SigE, Mycobacterium avium extracytoplasmic function alternative sigma factor (251 aa), fasta scores; opt: 226 z-score: 266.2 E(): 1.6e-07, 29.8% identity in 168 aa overlap 1 1 4 6 [Reporter]SCO1696 (16M12)_PCR/71 [Gene]SCO1696/131 NC_003888 SCI30A.17, unknown, len: 244 aa; similar to TR:P71976 (EMBL:Z81360) hypothetical protein from Mycobacterium tuberculosis (272 aa) fasta scores; opt: 192, z-score: 223.1, E(): 4.3e-05, (26.7% identity in 236 aa overlap) and to TR:Q98JP6 (EMBL:AP002998) Rhizobium loti (Mesorhizobium loti) MLL1845 241 aa; fasta scores: opt: 534 Z-score: 595.0 E(): 1.7e-25; 41.564% identity in 243 aa overlap 1 1 4 5 [Reporter]SCO3562 (17I12)_PCR/70 [Gene]SCO3562/129 NC_003888 SCH5.25, possible integral membrane transport, len: 830aa; contains possible membrane spanning hydrophobic regions. C-terminal region (600-800aa) similar to carbonic anhydrases eg. SW:CYNT_ECOLI carbonic anhydrase from Escherichia coli fasta scores; opt: 342, z-score: 333.5, E(): 2.9e-11, (35.7% identity in 210 aa overlap). Also N-terminal half has weak similarity to several proposed sulphate transporters e.g TR:Q55814 (EMBL:D64004) low affinity sulphate transporter from Synechocystis sp. PCC6803 fasta scores; opt: 419, z-score: 400.7, E(): 5.2e-15, (30.0% identity in 420 aa overlap). Contains Pfam matches to entry PF00484 Pro_CA, Prokaryotic-type carbonic anhydrases, score 131.20, E-value 5.4e-37 and entry PF00916 Sulfate_transp, Sulfate transporter family, score 119.90, E-value 4.9e-32. 1 2 10 2 [Reporter]SCO5115 (20A15)_PCR/2215 [Gene]SCO5115/4041 NC_003888 SCBAC31E11.11, bldKD, putative ABC transporter intracellular ATPase subunit, len: 353 aa; almost identical to previously sequenced TR:P72409 (EMBL:U680360) Streptomyces coelicolor BldKD putative ABC transporter intracellular ATPase subunit, 353 aa and similar to TR:O86574 (EMBL:AL031184) Streptomyces coelicolor SC2A11.13, 366 aa; fasta scores: opt: 1427 Z-score: 1344.1 bits: 257.3 E(): 3.8e-69; 60.340% identity in 353 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 1 4 4 [Reporter]SCO0292 (18E12)_PCR/69 [Gene]SCO0292/127 NC_003888 SC5G9.01, probable sugar ABC-transporter integral membrane protein, partial CDS, len: >148 aa; similar to e.g. TR:O51925 (EMBL:AF012836), malG, Thermococcus litoralis inner membrane protein from trehalose/maltose ABC-transporter system (278 aa), fasta scores; opt: 296 z-score: 384.7 E(): 4.6e-14, 32.2% identity in 146 aa overlap. Also similar to many putative ABC-transporter components e.g. SW:YOR2_CALSR (EMBL:L18965) Caldicellulosiruptor sp. (strain Rt8B.4) putative membrane bound sugar transport protein ORF2 (275 aa) (43.0% identity in 142 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component,SCF85.20, possible transmembrane transport protein, partial CDS, len: >185 aa. Similar to Streptomyces lividans TR:Q9ZI16 (EMBL; AF043654) BxlG (272 aa), fasta scores opt: 377 z-score: 450.9 E(): 9.2e-18 39.9% identity in 158 aa overlap and Streptomyces coelicolor TR:O50501 (EMBL; AL009199) probable transmembrane transport protein SCSt7b7.04 (302 aa), fasta scores opt: 288 z-score: 346.2 E(): 6.2e-12 29.3% identity in 181 aa overlap. Contains multiple potential membrane spanning hydrophobic domains. 1 1 4 3 [Reporter]SCP1.146 (19A12)_PCR/68 [Gene]SCP1.146/125 NC_003888 SCP1.146, possible ATP/GTP-binding protein, len: 848aa; weak but full length similarity with TR:Q9L4H0 (EMBL:AJ243106) conjugative protein from integrative element ICESt1 of Streptococcus thermophilus (834 aa) fasta scores; opt: 190, z-score: 212.3, E(): 0.00023, 18.6% identity in 870 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 1 4 2 [Reporter]SCO4055 (20I8)_PCR/67 [Gene]SCO4055/123 NC_003888 2SCD60.21c, probable alcohol dehydrogenase, len: 377 aa; similar to SW:FADH_PSEPU (EMBL:D21201) Pseudomonas putida glutathione-independent formaldehyde dehydrogenase (EC 1.2.1.46) FdhA, 398 aa; fasta scores: opt: 1048 z-score: 1080.0 E(): 0; 47.9% identity in 390 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and match to Prosite entry PS00059 Zinc-containing alcohol dehydrogenases signature 1 1 3 22 [Reporter]SCO4195 (1I4)_PCR/65 [Gene]SCO4195/121 NC_003888 2SCD46.09, possible ATP/GTP binding protein, len: 684aa; similar to TR:CAB93076 (EMBL:AL357432) putative ATP/GTP binding protein from Streptomyces coelicolor (681 aa) fasta scores; opt: 1940, z-score: 2059.2, E(): 0, 49.6% identity in 682 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 4 15 18 [Reporter]SCO2081 (5I9)_PCR/6372 [Gene]SCO2081/11611 NC_003888 SC4A10.14c, conserved hypothetical protein, len: 242 aa; unknown function, similar to many hypothetical proteins e.g. SW:YFIH_STRGR (EMBL:U07344) Streptomyces griseus hypothetical protein (246 aa), fasta scores; opt: 1246 z-score: 1349.0 E(): 0 76.3% identity in 245 aa overlap and SW:YFIH_ECOLI (EMBL:U50134), YfiH, Escherichia coli hypothetical protein (243 aa) (38.5% identity in 247 aa overlap) 1 2 9 20 [Reporter]SCO4701 (3I7)_PCR/2211 [Gene]SCO4701/4035 NC_003888 SCD31.26, rpsJ, 30S ribosomal protein S10, len: 102 aa; highly similar to SW:RS10_ECOLI (EMBL:V00344) Escherichia coli 30S ribosomal protein S10 RpsJ, 103 aa; fasta scores: opt: 428 z-score: 571.1 E(): 2.2e-24; 60.8% identity in 102 aa overlap. Contains Pfam match to entry PF00338 Ribosomal_S10, Ribosomal protein S10p/S20e and match to Prosite entry PS00361 Ribosomal protein S10 signature 1 2 9 18 [Reporter]SCO5728 (5A7)_PCR/2209 [Gene]SCO5728/4033 NC_003888 SC3C3.14c, probable secreted protein, len: 212 aa; contains N-terminal signal sequence 1 1 3 21 [Reporter]SCO0774 (2E4)_PCR/64 [Gene]SCO0774/119 NC_003888 3SCF60.06c, probable cytochrome P450, len: 412 aa; almost identical to TR:O85697 (EMBL:AF072709) Streptomyces lividans putative cytochrome P450 oxidoreductase, 406 aa and highly similar to SW:CPXH_STRGR (EMBL:X63601) Streptomyces griseus cytochrome P450 SoyC, 412 aa; fasta scores: opt: 1894 z-score: 2107.2 E(): 0; 67.5% identity in 412 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450 and match to Prosite entry PS00086 Cytochrome P450 cysteine heme-iron ligand signature 1 2 9 17 [Reporter]SCO6667 (6M3)_PCR/2208 [Gene]SCO6667/4031 NC_003888 SC5A7.17c, probable two-component regulator, len: 222 aa; highly similar to many e.g. DEGU_BACSU transcriptional regulatory protein DegU (229 aa), fasta scores; opt: 561 z-score: 630.7 E(): 7e-28, 44.0% identity in 225 aa overlap. Contains PS00622 Bacterial regulatory proteins, luxR family signature, probable helix-turn-helix motif from aa 173 to 194 (Score 1001, +2.60 SD) and Pfam matches to entry PF00072 response_reg, Response regulator receiver domain, score 124.10, E-value 2.7e-33 and entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 86.30, E-value 6.1e-22 1 1 3 20 [Reporter]SCO5441 (3A4)_PCR/63 [Gene]SCO5441/117 NC_003888 SC6A11.17c, pep2A, hypothetical protein, len: 464 aa; previously sequenced as TR:O54204 (EMBL:AJ001205) 1 1 3 19 [Reporter]SCO6150 (3M24)_PCR/62 [Gene]SCO6150/115 NC_003888 SC1A9.14, possible ADA-like regulatory protein, len: 477 aa; similar to ADA_MYCTU (EMBL:Z73902) putative ADA regulatory protein from Mycobacterium tuberculosis (496 aa) fasta scores; opt: 1135, z-score: 1376.3, E(): 0, (51.6% identity in 486 aa overlap). Also note N-terminal region similar to N-terminal region of ADA_ECOLI (EMBL:M10211) ADA regulatory protein from Escherichia coli (354 aa) fasta scores; opt: 302, z-score: 255.8, E(): 5.7e-07, (34.5% identity in 171 aa overlap) and C-terminal region similar to 3MG2_ECOLI AlkA, 3-methyladenine DNA glycosylase II involved in DNA repair (282 aa) fasta scores; opt: 171, z-score: 232.9, E(): 1.1e-05, (30.0% identity in 290 aa overlap). Contains Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family, score 72.90, E-value 6.7e-18 and PS00041 Bacterial regulatory proteins, araC family signature. 1 1 3 18 [Reporter]SCO7082 (4I24)_PCR/61 [Gene]SCO7082/113 NC_003888 SC3A4.08c, doubtful CDS, len: 154 aa. 1 1 3 17 [Reporter]SCO6560 (5E24)_PCR/60 [Gene]SCO6560/111 NC_003888 SC4B5.10c, respiratory chain oxidoreductase, len: 646 aa; C-terminal region is similar to the N-terminal region of many proteins involved in electron transfer e.g. TR:O27592 (EMBL:AE000915) NADP-reducing hydrogenase, subunit C from Methanobacterium thermoautotrophicum (630 aa), fasta scores; opt: 121,4 z-score: 1170.7, E(): 0, (47.1% identity in 397 aa overlap). Contains PS00645 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. 1 1 3 16 [Reporter]SCO6730 (6A24)_PCR/59 [Gene]SCO6730/109 NC_003888 SC5F2A.13, possible racemase, len: 398aa; similar to many egs. TR:O06543 (EMBL:Z95584) hypothetical protein from Mycobacterium tuberculosis (360 aa) fasta scores; opt: 1338, z-score: 1408.4, E(): 0, (57.1% identity in 361 aa overlap) and TR:O09176 (EMBL:U89906) alpha-methylacyl-CoA racemase from Mus musculus (moues) (361 aa) fasta scores; opt: 953, z-score: 1005.2, E(): 0, (44.2% identity in 344 aa overlap). Also similar to SC6A5.34 (EMBL:AL049485) probable fatty acid co-A racemase from Streptomyces coelicolor (387 aa) fasta scores; opt: 1787, z-score: 1558.9, E(): 0, (69.9% identity in 382 aa overlap). 1 2 9 12 [Reporter]SCO2768 (10I23)_PCR/2203 [Gene]SCO2768/4021 NC_003888 SCC57A.39c, unknown, len: 139 aa. 1 1 3 15 [Reporter]SCO4422 (7M20)_PCR/58 [Gene]SCO4422/107 NC_003888 SC6F11.20, possible hydrolase, len: 252 aa; similar to TR:Q9X7R7 (EMBL:AL049863) Streptomyces coelicolor putative hydrolase SC5H1.09c, 265 aa; fasta scores: opt: 465 z-score: 555.8 E(): 1.9e-23; 40.1% identity in 252 aa overlap 1 1 3 14 [Reporter]SCO7176 (8I20)_PCR/57 [Gene]SCO7176/105 NC_003888 SC8A11.04c, probable secreted peptidase, len: 1253 aa; similar to TR:Q9RL54 (EMBL:AL121596) Streptomyces coelicolor probable secreted peptidase SCF51A.10, 1245 aa; fasta scores: opt: 1827 z-score: 1848.3 E(): 0; 37.2% identity in 1300 aa overlap, to TR:P95684 (EMBL:D83672) Streptomyces albogriseolus subtilisin-like protease, 1102 aa; fasta scores: opt: 1365 z-score: 1380.6 E(): 0; 33.4% identity in 1268 aa overlap and to Streptomyces coelicolor probable secreted peptidase SC8A11.16c, 1239 aa; fasta scores: opt: 1835 z-score: 1467.4 E(): 0; 46.9% identity in 1279 aa overlap. Contains Pfam matches to entries PF00082 Peptidase_S8, Subtilase family and PF02225 PA, PA domain and matches to Prosite entries PS00136 Serine proteases, subtilase family, aspartic acid active site, PS00137 Serine proteases, subtilase family, histidine active site and PS00138 Serine proteases, subtilase family, serine active site. Also contains possible N-terminal region signal peptide sequence 1 1 3 13 [Reporter]SCO7163 (9E20)_PCR/56 [Gene]SCO7163/103 NC_003888 SC9A4.25c, possible transferase, len: 270 aa; similar to TR:CAB87566 (EMBL:AJ277295) Sphingomonas sp. putative acyl transferase FldZ protein, 224 aa; fasta scores: opt: 286 z-score: 338.7 E(): 2.1e-11; 27.4% identity in 223 aa overlap 1 1 3 12 [Reporter]SCO2301 (10A20)_PCR/55 [Gene]SCO2301/101 NC_003888 SCC30.09c, hypothetical protein, len: 285 aa; similar to TR:O69481 (EMBL:AL023635) Mycobacterium leprae hypothetical 40.3 kD protein MLCB1243.36, 385 aa; fasta scores: opt: 414 z-score: 484.0 E(): 1.7e-19; 34.9% identity in 370 aa overlap. Contains Pfam match to entry PF01784 DUF34, Domain of unknown function 1 3 16 22 [Reporter]SCO1210 (1M14)_PCR/4383 [Gene]SCO1210/7985 NC_003888 2SCG58.10, probable transcriptional regulatory protein, len: 218 aa; similar to TR:O53757 (EMBL:AL021933) Mycobacterium tuberculosis putative regulatory protein MTV038.16c, 234 aa; fasta scores: opt: 214 z-score: 258.1 E(): 6.5e-07; 30.7% identity in 199 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 35..56 (+3.27 SD) 1 3 16 21 [Reporter]SCO6800 (2I14)_PCR/4382 [Gene]SCO6800/7983 NC_003888 SC1A2.09, kbl, 2-amino-3-ketobutyrate coenzyme A ligase, len: 397 aa. Highly similar to many ligases involved in L-threonine catabolism e.g. Escherichia coli SW:KBL_ECOLI(EMBL:X06690) 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (398 aa), fasta scores opt: 1612 z-score: 1822.6 E():0 64.0% identity in 389 aa overlap. Contains a Prosite hit to PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site and a Pfam match to entry PF00222 aminotran_2, Aminotransferases class-II. 1 3 16 20 [Reporter]SCO2221 (3E14)_PCR/4381 [Gene]SCO2221/7981 NC_003888 SC10B7.16, hypothetical protein, len: 296 aa; similar to TR:O33319 (EMBL:AL008967) Mycobacterium tuberculosis hypothetical 40.2 kD protein MTV002.42c, 356 aa; fasta scores: opt: 980 z-score: 1190.8 E(): 0; 51.2% identity in 281 aa overlap 1 3 16 19 [Reporter]SCO5480 (4A14)_PCR/4380 [Gene]SCO5480/7979 NC_003888 SC2A11.14, probable oligopeptide ABC transporter ATP-binding protein, len: 496 aa; similar to many e.g. APPF_BACSU oligopeptide transport ATP-binding protein (329 aa), fasta scores; opt: 1284 z-score: 1056.3 E(): 0, 56.4% identity in 326 aa overlap Also similar to S. coelicolor TR:P72410 (EMBL:U68036) bldKE (284 aa), fasta scores; opt: 1114 z-score: 1091.6 E(): 0, 59.3% identity in 280 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature and Pfam match to entry PF00005 ABC_tran, ABC transporters, score 202.90, E-value 4.8e-57. C-terminus contains 13 degenerate repeats of AS(D /S)DA 1 3 16 18 [Reporter]SCO2094 (5M10)_PCR/4379 [Gene]SCO2094/7977 NC_003888 SC4A10.27, possible regulatory protein, len: 337 aa; similar to many e.g. SW:MOXR_PARDE (EMBL:M57684), MoxR, Paracoccus denitrificans protein probably involved in the regulation of methanol dehydrogenase synthesis (339 aa), fasta scores; opt: 189 z-score:216.0 E(): 7.6e-05, 31.55% identity in 336 aa overlap. Similar to many hypothetical proteins e.g. TM1132 (EMBL:AE001771) Thermotoga maritima putative MoxR protein (305 aa) (52.1% identity in 303 aa overlap). Similar to TR:Q9Z538 (EMBL:AL035212) S.coelicolor possible regulatory protein (332 aa) (45.8% identity in 312 aa overlap) 1 3 16 17 [Reporter]SCO6517 (6I10)_PCR/4378 [Gene]SCO6517/7975 NC_003888 SC5C7.02, uvrA-like protein, len: 796 aa; similar to Streptomyces peucetius TR:Q54827 (EMBL:L76359) daunorubicin resistance protein drrC (764 aa), fasta scores; opt: 2051 z-score: 2702.2 E(): 0, 51.1% identity in 775 aa overlap, and to many uvrA proteins e.g. UVRA_NEIGO excinuclease ABC subunit A (950 aa), fasta scores; opt: 412 z-score: 1697.0 E(): 0, 34.1% identity in 953 aa overlap. Contains 2x PS00017 ATP /GTP-binding= match to entry PF00005 ABC_tran, ABC transporters, scores 42.40, E-value 1.7e-10 and 71.20, E-value 2.2e-17 1 3 16 16 [Reporter]SCO5688 (7E10)_PCR/4377 [Gene]SCO5688/7973 NC_003888 SC5H4.12, probable transport system integral membrane protein, len: 323 aa; similar to SW:LPLC_BACSU (EMBL:L19164) Bacillus subtilis LplC, 295 aa; fasta scores: opt: 669 z-score: 758.9 E(): 0; 38.0% identity in 276 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component. Contains also possible hydrophobic membrane spanning regions 1 3 16 15 [Reporter]SCO5314 (8A10)_PCR/4376 [Gene]SCO5314/7971 NC_003888 SC6G9.19, whiE ORF VII, len: 111 aa; unknown function, whiE locus protein previously sequenced from S. coelicolor as SW:YW12_STRCO (EMBL:X55942) 1 3 16 14 [Reporter]SCO7837 (9M6)_PCR/4375 [Gene]SCO7837/7969 NC_003888 SC8E7.34c, possible membrane protein, len: 363 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1Y4(EMBL:AL109973) hypothetical protein, SCJ30.05 (363 aa). Also similar to Streptomyces coelicolor TR:Q9X886(EMBL:AL049707) putative transferase, SCE15.11C (448 aa), fasta scores opt: 411 z-score: 409.1 E(): 2.6e-15 32.8% identity in 274 aa overlap. Note the large overlap with the adjoining CDS. Contains possible membrane spanning hydrophobic domains and a TTA /leucine codon, possible target for bldA regulation. 1 3 16 13 [Reporter]SCO2532 (10I6)_PCR/4374 [Gene]SCO2532/7967 NC_003888 SCC117.05, PhoH-like protein, len: 359 aa. Highly similar to many including: Escherichia coli SW:PHOH_ECOLI (EMBL:D10391) phosphate starvation-inducible protein PhoH protein (354 aa), fasta scores opt: 583 z-score: 650.4 E(): 7.8e-29 37.7% identity in 321 aa overlap and Mycobacterium tuberculosis SW:PHOL_MYCTU(EMBL:Z95208) PhoH-like protein (352 aa), fasta scores opt: 1407 z-score: 1560.0 E():0 63.6% identity in 349 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 16 12 [Reporter]SCO2287 (11E6)_PCR/4373 [Gene]SCO2287/7965 NC_003888 SCC75A.33, possible oxidoreductase, len: 263 aa. Similar to several including: Bacillus megaterium SW:DHG2_BACME (EMBL:D90044) glucose 1-dehydrogenase II (EC 1.1.1.47) (261 aa), fasta scores opt: 400 z-score: 442.6 E(): 2.8e-17 38.9% identity in 193 aa overlap SW:YOHF_ECOLI (EMBL:U00007) hypothetical oxidoreductase (253 aa), fasta scores opt: 422 z-score: 466.5 E(): 1.3e-18 38.3% identity in 206 aa overlap. Contains a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature and a Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 1 3 16 11 [Reporter]SCO4801 (12A6)_PCR/4372 [Gene]SCO4801/7963 NC_003888 SCD63A.12c, unknown, len: 606 aa. High content in alanine amino acid residues 1 3 16 10 [Reporter]SCO3035 (13M2)_PCR/4371 [Gene]SCO3035/7961 NC_003888 SCE34.16, hypothetical protein, len: 164 aa; similar to TR:O53413 (EMBL:AL021897) Mycobacterium tuberculosis hypothetical 20.6 kD protein MTV017.18, 188 aa; fasta scores: opt: 251 z-score: 321.5 E(): 1.9e-10; 39.6% identity in 154 aa overlap 1 3 16 9 [Reporter]SCO3807 (14I2)_PCR/4370 [Gene]SCO3807/7959 NC_003888 SCGD3.08c, possible hydrolase, len: 264 aa; weakly similar to hydrolases e.g. to TR:Q44185 (EMBL:X91070) Agrobacterium radiobacter D-N-alpha-carbamilase (304 aa) (28.17% identity in 252 aa overlap). Similar to many hypothetical proteins e.g. TR:O27839 (EMBL:AE000934) Methanobacterium thermoautotrophicum putative N-carbamoyl-D-amino acid amidohydrolase (272 aa) (36.2% identity in 265 aa overlap). Similar to others from S.coelicolor e.g. TR:O69808 (EMBL:AL023496) S.coelicolor possible hydrolase (280 aa) (31.7% identity in 281 aa overlap). An alternative start codon is present at codon 10. Contains PS01227 Uncharacterized protein family UPF0012 signature which is also present in many of the hypothetical proteins similar to this one 1 3 16 8 [Reporter]SCO3628 (15E2)_PCR/4369 [Gene]SCO3628/7957 NC_003888 SCH10.06c, putative secreted protein, len: 163 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains possible N-terminal region signal peptide sequence 1 3 16 7 [Reporter]SCO1566 (16A2)_PCR/4368 [Gene]SCO1566/7955 NC_003888 SCL24.02, possible acyltransferase, len: 223 aa; similar to TR:Q9ZBS1 (EMBL:AL034447) Streptomyces coelicolor putative acyltransferase SC7A1.02, 264 aa; fasta scores: opt: 277 z-score: 327.1 E(): 8.5e-11; 30.7% identity in 202 aa overlap and to TR:CAB51970 (EMBL:AL109661) Streptomyces coelicolor hypothetical 28.2 kD protein SC6E10.16c, 262 aa; fasta scores: opt: 858 z-score: 879.9 E(): 0; 59.0% identity in 217 aa overlap. Contains Pfam match to entry PF01553 Acyltransferase, Acyltransferase 1 3 16 6 [Reporter]SCO0708 (16M22)_PCR/4367 [Gene]SCO0708/7953 NC_003888 SCF42.18c, possible branched-chain amino acid ABC transport protein, len: 299 aa. Similar to many other transport proteins including: Pseudomonas aeruginosa SW:BRAD_PSEAE(EMBL:D90223) high-affinity branched-chain amino acid transport protein, BraD (307 aa), fasta scores opt: 318 z-score: 364.2 E(): 6.8e-13 28.5% identity in 312 aa overlap and Deinococcus radiodurans TR:AAF09863(EMBL:AE001889) branched-chain amino acid ABC transporter, permease protein (285 aa), fasta scores opt: 432 z-score: 492.1 E(): 5.1e-20 34.6% identity in 295 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 1 3 16 5 [Reporter]SCO3639 (17I22)_PCR/4366 [Gene]SCO3639/7951 NC_003888 SCH10.17c, probable two-component system sensor kinase, len: 472 aa; similar to many e.g. TR:Q53893 (EMBL:U51332), AbsA1, Streptomyces coelicolor histidine kinase homolog involved in negative regulation of antibiotic synthesis (571 aa), fasta scores; opt: 370 z-score: 410.4 E(): 1.5e-15, 29.6% identity in 375 aa overlap. Also similar to SW:NARX_ECOLI (EMBL:X13360), NarX, NarR, Escherichia coli nitrate/nitrite sensor protein (598 aa) (28.5% identity in 228 aa overlap). Contains hydrophobic, probable membrane-spanning regions. Similar to many other putative two-component system sensor kinases from Streptomyces coelicolor e.g. SCH10.32c, probable two-component system sensor (441 aa) (38.0% identity in 389 aa overlap). Contains probable helix-turn-helix motif at aa 235-256 (Score 1122, +3.01 SD). The start codon may be further downstream, which would agree with some other bacterial two-component system sensors; however, NarX has a length comparable to this prediction 4 1 21 18 [Reporter]SCO7109 (5D20)_PCR/1969 [Gene]SCO7109/3599 NC_003888 SC4B10.10c, possible oxidoreductase, len: 576 aa; similar to TR:O27546 (EMBL:AE000910) Methanobacterium thermoautotrophicum succinate dehydrogenase, flavoprotein subunit MTH1502, 558 aa; fasta scores: opt: 1262 z-score: 1392.4 E(): 0; 41.0% identity in 542 aa overlap and to TR:O53141 (EMBL:AJ000941) Methanobacterium thermoautotrophicum strain marburg, thiol:fumarate reductase subunit A Tfr, 545 aa; fasta scores: opt: 1184 z-score: 1306.6 E(): 0; 40.5% identity in 536 aa overlap. Contains Pfam match to entry PF00890 FAD_binding_2, FAD binding domain 1 3 16 4 [Reporter]SCO4862 (18E22)_PCR/4365 [Gene]SCO4862/7949 NC_003888 SCK20.03, unknown, len: 189 aa 1 3 16 3 [Reporter]SCO1882 (19A22)_PCR/4364 [Gene]SCO1882/7947 NC_003888 SCI39.29c, probable secreted sugar-binding protein, len: 452 aa; similar to TR:P72397 (EMBL:Y07706) Streptomyces coelicolor putative maltose-binding protein MalE, 423 aa; fasta scores: opt: 433 Z-score: 459.6 bits: 94.2 E(): 5.2e-18; 30.716% identity in 433 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and possible N-terminal region signal peptide sequence 1 3 16 2 [Reporter]SCO5970 (20I18)_PCR/4363 [Gene]SCO5970/7945 NC_003888 SCBAC16H6.05, unknown, len: 156 aa: no significant database matches. Contains TTA-encoded leucine close to C-terminus. 1 3 15 22 [Reporter]SCO1206 (1I14)_PCR/4361 [Gene]SCO1206/7943 NC_003888 2SCG58.06, possible polyketide synthase, len: 374 aa; highly similar to TR:Q54240 (EMBL:AB018074) Streptomyces griseus ORF-1 protein similar to chalcone synthase, 372 aa; fasta scores: opt: 1769 z-score: 1998.4 E(): 0; 70.4% identity in 361 aa overlap and to SW:CHSJ_PETHY (EMBL:X14597) Petunia hybrida chalcone synthase J (EC 2.3.1.74) ChsJ, 389 aa; fasta scores: opt: 412 z-score: 469.3 E(): 1.1e-18; 29.5% identity in 312 aa overlap. Contains Pfam match to entry PF00195 Chal_stil_synt, Chalcone and stilbene synthases 1 3 15 21 [Reporter]SCO5676 (2E14)_PCR/4360 [Gene]SCO5676/7941 NC_003888 SC8B7.02, gabT, probable 4-aminobutyrate aminotransferase, len: 444 aa; highly similar to e.g. GABT_ECOLI 4-aminobutyrate aminotransferase (EC 2.6.1.19) (426 aa), fasta s cores; opt: 1194 z-score: 1404.8 E(): 0, 45.5% identity in 424 aa overlap. Contains PS00600 Aminotransferases class-II I pyridoxal-phosphate attachment site and Pfam match to ent ry PF00202 aminotran_3, Aminotransferases class-III pyridox al-phosphate, score 516.20, E-value 5e-179 4 1 21 13 [Reporter]SCO1921 (10P12)_PCR/1964 [Gene]SCO1921/3589 NC_003888 SCC22.03c, possible aminotransferase, len: 418 aa; similar to many proposed aminotransferase/nifS-like proteins e.g. TR:Q49690 (EMBL:U00013) NifS from Mycobacterium leprae (418 aa) fasta scores; opt: 1438, z-score: 1673.0, E(): 0, (53.4% identity in 414 aa overlap). Contains Pfam match to entry PF00266 aminotran_5, Aminotransferases class-V. 1 3 15 20 [Reporter]SCO2217 (3A14)_PCR/4359 [Gene]SCO2217/7939 NC_003888 SC10B7.12, probable secreted protein, len: 501 aa; similar to TR:CAB61161 (EMBL:AL132973) Streptomyces coelicolor putative membrane protein, SCF91.04c, 718 aa; fasta scores: opt: 1496 z-score: 1486.8 E(): 0; 55.1% identity in 523 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 15 19 [Reporter]SCO6948 (4M10)_PCR/4358 [Gene]SCO6948/7937 NC_003888 SC6F7.01, unknown, partial CDS, len:> 157 aa. Weakly similar to several including: Aeropyrum pernix TR:Q9YFC8 (EMBL:AP000059) hypothetical protein (168 aa), fasta scores opt: 231 z-score: 293.6 E(): 6.5e-09 35.8% identity in 137 aa overlap.,SC1G8.20, unknown, len: 183 aa. Weakly similar to several including: Aeropyrum pernix TR:Q9YFC8 (EMBL:AP000059) hypothetical protein (168 aa), fasta scores opt: 231 z-score: 290.8 E(): 9.8e-09 35.8% identity in 137 aa overlap. Overlaps and extends into CDS SC6F7.01 on the adjoining cosmid. 1 3 15 18 [Reporter]SCO2090 (5I10)_PCR/4357 [Gene]SCO2090/7935 NC_003888 SC4A10.23c, ftsI, cell division protein, len: 654 aa; previously sequenced as TR:Q9Z5V7 (EMBL:AF123319), FtsI, Streptomyces coelicolor cell division protein (651 aa) and identical to that sequence, but with a different putative start codon. C-terminal end shares weak similarity with others from S.coelicolor e.g. SC6G9.32 (EMBL:AL079356) S.coelicolor possible penicillin-binding protein (485 aa) (29.3% identity in 447 aa overlap). Contains a hydrophobic, possible membrane-spanning region. Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and two PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 15 17 [Reporter]SCO5789 (6E10)_PCR/4356 [Gene]SCO5789/7933 NC_003888 SC4H2.10c, unknown, len: 97 aa 1 3 15 16 [Reporter]SCO5684 (7A10)_PCR/4355 [Gene]SCO5684/7931 NC_003888 SC5H4.08, probable two-component system response regulator, len: 237 aa; similar to TR:Q9X8Q7 (EMBL:AL049754) Streptomyces coelicolor putative two-component system regulator SCH10.18c, 228 aa; fasta scores: opt: 780 z-score: 922.5 E(): 0; 58.7% identity in 225 aa overlap and to TR:BAA88407 (EMBL:AB016841) Streptomyces thermoviolaceus ChiR protein, 213 aa; fasta scores: opt: 567 z-score: 674.0 E(): 4.4e-30; 46.9% identity in 213 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature and also a possible helix-turn-helix motif at residues 180..201 (+2.70 SD) 4 1 21 8 [Reporter]SCO0531 (15L8)_PCR/1959 [Gene]SCO0531/3579 NC_003888 SCF11.11, probable sugar transporter sugar binding protein, len: 422 aa; similar to TR:P72397 (EMBL:Y07706) Streptomyces coelicolor MalE, putative maltose-binding protein, 423 aa; fasta scores: opt: 367 z-score: 390.0 E(): 2.4e-14; 27.2% identity in 419 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, bacterial extracellular solute-binding protein and a prosite match correctly situated to entry PS00013 prokaryotic membrane lipoprotein lipid attachment site. Contains probable N-terminal signal sequence 2 4 4 21 [Reporter]SCO5367 (2J1)_PCR/6637 [Gene]SCO5367/12093 NC_003888 2SC6G5.11, atpB, ATP synthase A chain (EC 3.6.1.34), len: 273aa; similar to many eg. SW:P15012 (ATP6_RHORU) ATP synthase A chain from Rhodospirillum rubrum (241 aa) fasta scores; opt: 356, z-score: 432.3, E(): 1.3e-16, 35.7% identity in 252 aa overlap. Identical to SW:P50012 (ATP6_STRLI) ATP synthase A chain from Streptomyces lividans. Contains Pfam match to entry PF00119 ATP-synt_A, ATP synthase A chain and Prosite match to PS00449 ATP synthase a subunit signature. Contains possible membrane-spanning hydrophobic regions. 2 4 4 20 [Reporter]SCO4574 (3F1)_PCR/6636 [Gene]SCO4574/12091 NC_003888 SCD16A.09c, nuoM, NADH dehydrogenase subunit, len: 523 aa; similar to many e.g. TR:O53307 (EMBL:AL021646) NuoM, NADH dehydrogenase subunit from Mycobacterium tuberculosis (553 aa) fasta scores; opt: 1621, z-score: 1842.0, E(): 0, (56.6% identity in 541 aa overlap) and SW:NUOM_ECOLI, NADH dehydrogenase subunit NuoM from Escherichia coli (509 aa) fasta scores; opt: 970, z-score: 1103.8, E(): 0, (35.1% identity in 513 aa overlap). Contains Pfam match to entry PF00361 oxidored_q1, NADH-Ubiquinone/plastoquinone (complex I), various chains. Contains possible membrane spanning hydrophobic regions. 1 3 15 15 [Reporter]SCO2467 (8M6)_PCR/4354 [Gene]SCO2467/7929 NC_003888 SC7A8.06, hypothetical protein, len: 333 aa; identical to previously sequenced TR:CAB51552 (EMBL:AJ244019) Streptomyces coelicolor A3(2) hypothetical 37.1 kD protein, 333 aa 1 3 15 14 [Reporter]SCO7831 (9I6)_PCR/4353 [Gene]SCO7831/7927 NC_003888 SC8E7.28c, unknown, len: 67 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1X7(EMBL:AL109973) hypothetical 7.2 kd protein, SCJ30.12 (67 aa). 1 3 15 13 [Reporter]SCO5709 (10E6)_PCR/4352 [Gene]SCO5709/7925 NC_003888 SC9F2.07c, probable tRNA pseudouridine synthase, len: 301 aa; similar to many e.g. SW:TRUB_ECOLI tRNA pseudouridine synthase B from Escherichia coli (314 aa) fasta scores; opt: 357, z-score: 415.7, E(): 7.6e-16, (37.6% identity in 234 aa overlap). 1 3 15 12 [Reporter]SCO2283 (11A6)_PCR/4351 [Gene]SCO2283/7923 NC_003888 SCC75A.29c, possible secreted esterase, len: 351 aa. Similar, in parts, to several esterases e.g. Pseudomonas sp. TR:Q52467(EMBL:M68491) esterase (389 aa), fasta scores opt: 256 z-score: 289.8 E(): 9.2e-09 30.3% identity in 323 aa overlap. Also similar, in parts, to several penicillin-binding proteins e.g. Nocardia lactamdurans SW:PBP4_NOCLA (EMBL:Z13972) penicillin-binding protein 4 (PBP-4) (381 aa), fasta scores opt: 249 z-score: 282.1 E(): 2.4e-08 29.2% identity in 308 aa overlap. Contains a possible N-terminal signal sequence. 1 3 15 11 [Reporter]SCO4120 (12M2)_PCR/4350 [Gene]SCO4120/7921 NC_003888 SCD72A.06, conserved hypothetical protein, len: 552 aa; similar in regions to many from Streptomyces coelicolor eg. TR:Q9ZBJ0 (EMBL:AL035161) conserved hypothetical protein (860 aa) fasta scores; opt: 906, z-score: 1019.8, E(): 0, 43.5% identity in 370 aa overlap 4 1 21 3 [Reporter]SCP1.24 (20H4)_PCR/1954 [Gene]SCP1.24/3569 NC_003888 SCP1.24, unknown, doubtful CDS, len: 81aa; 2 4 4 18 [Reporter]SCO6229 (4N21)_PCR/6634 [Gene]SCO6229/12087 NC_003888 SC2H4.11c, probable sugar transport system permease protein, len: 293 aa; similar to many e.g. MALD_STRPN maltodextrin transport system permease protein (277 aa), fasta scores; opt: 394 z-score: 585.4 E(): 2.3e-25, 29.1% identity in 268 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 58.30, E-value 1.6e-13 2 4 4 17 [Reporter]SCO7049 (5J21)_PCR/6633 [Gene]SCO7049/12085 NC_003888 SC4G1.15, probable glutaminase, len: 307 aa; similar to TR:O87405 (EMBL:AF057158) Rhizobium etli glutaminase A, 309 aa; fasta scores: opt: 990 z-score: 1158.2 E(): 0; 52.3% identity in 304 aa overlap and to middle part of SW:GLSK_RAT (EMBL:M65150) Rattus norvegicus glutaminase, kidney isoform precursor (EC 3.5.1.2) GlsK, 647 aa; fasta scores: opt: 577 z-score: 672.7 E(): 5.3e-30; 35.2% identity in 293 aa overlap 2 4 4 16 [Reporter]SCO7581 (6F21)_PCR/6632 [Gene]SCO7581/12083 NC_003888 SC5F1.35c, hypothetical protein, len: 556 aa; low similarity to SW:Y811_METJA (EMBL:U67525) Methanococcus jannaschii hypothetical protein MJ0811, 439 aa; fasta scores: opt: 321 z-score: 347.7 E(): 7.7e-12 26.8% identity in 287 aa overlap 2 4 4 15 [Reporter]SCO7417 (7B21)_PCR/6631 [Gene]SCO7417/12081 NC_003888 SC6D11.13c, possible cytochrome P450-family protein, len: 407 aa. Similar to many members of the cytochrome P450 family including: Streptomyces griseus SW:CPXH_STRGR (EMBL:X63601) cytochrome P450-soy (412 aa), fasta scores opt: 673 z-score: 761.0 E():0 35.1% identity in 382 aa overlap and Saccharopolyspora erythraea (Streptomyces erythraeus) SW:CPXK_SACER (EMBL:M83110) cytochrome P450 107B1 (405 aa), fasta scores opt: 872 z-score: 984.9 E():0 38.4% identity in 409 aa overlap. Contains a Prosite hit to PS00086 Cytochrome P450 cysteine heme-iron ligand signature and a Pfam match to entry PF00067 p450, Cytochrome P450. 1 3 15 10 [Reporter]SCO3030 (13I2)_PCR/4349 [Gene]SCO3030/7919 NC_003888 SCE34.11c, hypothetical protein, len: 140 aa; similar to TR:O53351 (EMBL:AL021840) Mycobacterium tuberculosis hypothetical 16.8 kD protein MTV015.30c, 163 aa; fasta scores: opt: 353 z-score: 430.4 E(): 1.6e-16; 48.3% identity in 147 aa overlap 1 3 15 9 [Reporter]SCO0256 (14E2)_PCR/4348 [Gene]SCO0256/7917 NC_003888 SCF20.02, possible short chain oxidoreductase, len: 265aa; similar to many oxidoreductases e.g. TR:Q9ZNN8 (EMBL:AB009078) L-2,3-butanediol dehydrogenase from Corynebacterium glutamicum (258 aa) fasta scores; opt: 784, z-score: 848.0, E(): 0, (49.4% identity in 255 aa overlap). Contains Pfam matches to entry PF00106 adh_short, short chain dehydrogenase and to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature 1 3 15 8 [Reporter]SCO0150 (15A2)_PCR/4347 [Gene]SCO0150/7915 NC_003888 SCJ33.14, unknown, len: 164 aa 1 3 15 7 [Reporter]SCO1027 (15M22)_PCR/4346 [Gene]SCO1027/7913 NC_003888 SCG20A.07, hypothetical protein, len: 157 aa; similar to SW:Y0BR_MYCTU (EMBL:Z77724) Mycobacterium tuberculosis hypothetical 19.0 kD protein CY227.27c, 167 aa; fasta scores: opt: 218 z-score: 283.7 E(): 2.5e-08; 30.7% identity in 150 aa overlap 1 3 15 6 [Reporter]SCO0917 (16I22)_PCR/4345 [Gene]SCO0917/7911 NC_003888 SCM10.05, possible oxygenase, len: 339 aa; similar to TR:Q9X2P4 (EMBL:AF041061) Mycobacterium smegmatis alkanal monooxygenase-like hypothetical protein AmlP, 289 aa; fasta scores: opt: 269 z-score: 305.4 E(): 1.3e-09; 29.2% identity in 291 aa overlap and to SW:LUXA_VIBHA (EMBL:M10961) Vibrio harveyi alkanal monooxygenase alpha chain (EC 1.14.14.3) LuxA, 355 aa; fasta scores: opt: 208 z-score: 236.6 E(): 8.6e-06; 27.8% identity in 205 aa overlap. Contains Pfam match to entry PF00296 bac_luciferase, Bacterial luciferase 4 1 20 19 [Reporter]SCO5623 (4D20)_PCR/1948 [Gene]SCO5623/3559 NC_003888 SC2E1.40c, probable secreted protein, len: 226 aa; some similarity to two putative secreted M. tuberculosis proteins YX22_MYCTU MTCY274.22 (249 aa), fasta scores; opt: 315 z-score: 268.8 E(): 9.7e-08, 40.7% identity in 145 aa overlap (also upstream of rpsB) and Y0GK_MYCTU MTCY13D12.20 (407 aa), fasta scores; opt: 218 z-score: 176.1 E(): 0.014, 34.1% identity in 164 aa overlap. Contains probable N-terminal signal sequence. 1 3 15 5 [Reporter]SCO5176 (17E22)_PCR/4344 [Gene]SCO5176/7909 NC_003888 SCP8.39, probable reductase, len: 370 aa; similar to TR:O54256 (EMBL:AJ224512) Streptomyces nogalater putative dTDP-4-keto-6-deoxyhexose reductase SnoG, 328 aa; fasta scores: opt: 316 z-score: 337.4 E(): 2.5e-11; 30.5% identity in 364 aa overlap 1 3 15 4 [Reporter]SCO2884 (18A22)_PCR/4343 [Gene]SCO2884/7907 NC_003888 SCE6.21, possible cytochrome P450, len: 408 aa. Highly similar to many other cytochrome P450 proteins including: Saccharopolyspora erythraea (Streptomyces erythraeus) SW:CPXJ_SACER(EMBL:X60379) 6-deoxyerythronolide B hydroxylase (404 aa), fasta scores opt: 777 z-score: 888.8 E():0 37.8% identity in 410 aa overlap and Streptomyces fradiae TR:Q9XCC6(EMBL:AF145049) (433 aa), fasta scores opt: 1253 z-score: 1430.3 E():0 50.9% identity in 409 aa overlap. Also similar to the adjoining CDS SCE6.20 (410 aa), fasta scores opt: 257 z-score: 252.4 E(): 1.5e-08 26.5% identity in 407 aa overlap. Contains a PS00086 Cytochrome P450 cysteine heme-iron ligand signature and a Pfam match to entry PF00067 p450, Cytochrome P450. 1 3 15 3 [Reporter]SCP1.269 (19M18)_PCR/4342 [Gene]SCP1.269/7905 NC_003888 SCP1.269, possible secreted protein, len: 123aa; contains a possible cleavable N-terminal signal sequence. 1 3 15 2 [Reporter]SCO2810 (20E18)_PCR/4341 [Gene]SCO2810/7903 NC_003888 SCBAC17F8.01, unknown, len: 220aa: no significant database matches.,2SCC13.18, unknown (fragment), len: >268 aa 1 3 14 22 [Reporter]SCO0989 (1E14)_PCR/4339 [Gene]SCO0989/7901 NC_003888 2SCG2.02c, unknown, len: 134 aa 4 1 20 14 [Reporter]SCO5409 (9P12)_PCR/1943 [Gene]SCO5409/3549 NC_003888 SC8F4.13, putative membrane protein, len: 114 aa; similar to TR:O31485 (EMBL:Z99106) hypothetical protein from Bacillus subtilis (96 aa) fasta scores; opt: 179, z-score: 259.7, E(): 4.7e-07, 37.4% identity in 91 aa overlap. Contains possible hydrophobic membrane spanning regions 2 4 4 7 [Reporter]SCO3223 (15B13)_PCR/6623 [Gene]SCO3223/12065 NC_003888 SCE8.16c, probable ABC transporter integral membrane protein, len: 266aa; located directly downstream of a probable ABC transporter ATP-binding protein (6bp gap). Similar to many particularly TR:O86630 (EMBL:AL031155) an integral membrane protein located directly downstream of a probable ABC transporter ATP-binding protein (2bp gap) from Streptomyces coelicolor (279 aa) fasta scores; opt: 460, z-score: 539.7, E(): 9.4e-23, (37.0% identity in 273 aa overlap). Contains several probable membrane spanning hydrophobic regions. 2 4 4 6 [Reporter]SCO1789 (16N9)_PCR/6622 [Gene]SCO1789/12063 NC_003888 SCI51.29, hypothetical protein, len: 430 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 2 4 4 5 [Reporter]SCO3918 (17J9)_PCR/6621 [Gene]SCO3918/12061 NC_003888 SCQ11.01c, hypothetical protein, partial CDS, len: >95 aa; unknown function, previously sequenced as TR:Q53870 (EMBL:U37580), S.coelicolor hypothetical protein ORF5 downstream of phosphotyrosine protein phosphatase (159 aa). Weak similarity to the N-terminus of TR:O69888 (EMBL:AL023797) Streptomyces coelicolor hypothetical protein (172 aa), fasta scores; opt: 158 z-score: 206.4 E(): 0.00036, 46.2% identity in 65 aa overlap. Continues as SCH24.40c in cosmid H24 (EMBL:AL049826),SCH24.40c, hypothetical protein, partial CDS, len: >96 aa; unknown function, highly similar to TR:Q53870 (EMBL:U37580) S.coelicolor hypothetical protein ORF5 downstream of phosphotyrosine protein phosphatase (159 aa), fasta scores; opt: 615 z-score: 859.5 E(): 0, 94.8% identity in 96 aa overlap 4 1 20 8 [Reporter]SCO0558 (15H8)_PCR/1937 [Gene]SCO0558/3537 NC_003888 SCF73.05c, unknown, len: 88 aa 4 1 20 7 [Reporter]SCO1553 (16D8)_PCR/1936 [Gene]SCO1553/3535 NC_003888 SCL11.09c, probable uroporphyrin-III methyltransferase, len: 410 aa; similar to TR:O05812 (EMBL:Z95207) Mycobacterium tuberculosis CysG, 405 aa; fasta scores: opt: 1487 z-score: 1630.7 E(): 0; 58.7% identity in 409 aa overlap and to SW:SUMT_BACME (EMBL:M62881) Bacillus megaterium uroporphyrin-III C-methyltransferase (EC 2.1.1.107) CobA, 238 aa; fasta scores: opt: 719 z-score: 794.9 E(): 0; 48.1% identity in 233 aa overlap. Contains Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases and match to Prosite entry PS00840 Uroporphyrin-III C-methyltransferase signature 2 2 4 4 4 [Reporter]SCO1590 (18F9)_PCR/6620 [Gene]SCO1590/12059 NC_003888 SCI35.12c, putative secreted protein, len: 414 aa; similar to hypothetical proteins from several organisms e.g. E. coli TR:P76130 (EMBL:AE000246) (439 aa), fasta scores; opt: 879 z-score: 1103.6 E(): 0, 39.6% identity in 407 aa overlap. Contains possible N-terminal region signal peptide sequence 2 4 4 3 [Reporter]SCP1.89c (19B9)_PCR/6619 [Gene]SCP1.89c/12057 NC_003888 SCP1.89c, unknown, len: 389aa; 2 4 4 2 [Reporter]SCO1362 (20J5)_PCR/6618 [Gene]SCO1362/12055 NC_003888 SC10A9.04c, possible integral membrane protein, len: 324 aa; similar to SW:YWFM_BACSU (EMBL:X73124) Bacillus subtilis hypothetical 31.3 kDa protein in Pta 3' region Ywfm, 296 aa; fasta scores: opt: 267 z-score: 282.4 E(): 2.7e-08; 26.9% identity in 305 aa overlap. Contains 2x Pfam matches to entry PF00892 DUF6, Integral membrane protein DUF6. Also contains possible hydrophobic membrane spanning regions and a 4x degenerate repeat: LRP(R/A) 2 4 3 22 [Reporter]SCO4533 (1J1)_PCR/6616 [Gene]SCO4533/12053 NC_003888 2SCD4.04, hypothetical protein, len: 108 aa; similar to TR:CAB77307 (EMBL:AL160312) Streptomyces coelicolor hypothetical 13.8 kDa protein SCD35.23c, 119 aa; fasta scores: opt: 290 z-score: 364.1 E(): 8.2e-13; 47.6% identity in 105 aa overlap 2 4 3 21 [Reporter]SCO0725 (2F1)_PCR/6615 [Gene]SCO0725/12051 NC_003888 3SC5B7.03, unknown, len: 125 aa 4 1 20 3 [Reporter]SCO4905 (20D4)_PCR/1932 [Gene]SCO4905/3527 NC_003888 2SCK8.31c, possible lipoprotein, len: 203 aa; similar to TR:Q9K453 (EMBL:AL359215) Streptomyces coelicolor putative secreted protein SC2H12.31, 211 aa; fasta scores: opt: 142 z-score: 175.4 E(): 0.033; 29.3% identity in 205 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 1 20 2 [Reporter]SCO2816 (20L24)_PCR/1931 [Gene]SCO2816/3525 NC_003888 SCBAC17F8.07, conserved hypothetical protein, len: 366 aa; similar to many e.g. TR:Q9I0Z9 (EMBL:AE004676) hypothetical protein from Pseudomonas aeruginosa (333 aa) fasta scores; opt: 588, Z-score: 637.0, 47.826% identity (50.305% ungapped)in 345 aa overlap 2 4 3 20 [Reporter]SCO4572 (3B1)_PCR/6614 [Gene]SCO4572/12049 NC_003888 SCD16A.11c, nuoK, NADH dehydrogenase subunit, len: 99 aa; similar to many e.g. SW:NUOK_MYCTU NuoK, NADH dehydrogenase subunit from Mycobacterium tuberculosis (99 aa) fasta scores; opt: 503, z-score: 724.2, E(): 5.2e-33, (77.6% identity in 98 aa overlap) and SW:NUOK_ECOLI, NADH dehydrogenase subunit NuoK from Escherichia coli (100 aa) fasta scores; opt: 253, z-score: 373.2, E(): 1.8e-13, (40.2% identity in 97 aa overlap). Contains Pfam match to entry PF00420 oxidored_q2, NADH-ubiquinone/plastoquinone oxidoreductase chain 4L. Contains possible membrane spanning hydrophobic regions. 2 4 3 19 [Reporter]SCO1396 (3N21)_PCR/6613 [Gene]SCO1396/12047 NC_003888 SC1A8A.16c, probable D-alanyl-D-alanine dipeptidase (putative secreted protein), len: 274aa; similar to many eg. TR:Q9X523 (EMBL:AF120672) pcgL, D-alanyl-D-alanine dipeptidase from Salmonella typhimurium (256 aa) fasta scores; opt: 851, z-score: 983.2, E(): 0, 49.6% identity in 252 aa overlap. Contains two Pfam matches to entry PF01427 Peptidase_M45, D-ala-D-ala dipeptidase and possible N-terminal region signal peptide sequence 2 4 3 18 [Reporter]SCO6225 (4J21)_PCR/6612 [Gene]SCO6225/12045 NC_003888 SC2H4.07c, probable secreted protein, len: 421 aa; similar in C-terminus to B. subtilis TR:P70960 (EMBL:Z81356;) hypothetical protein ywmC precursor (227 aa), fasta scores; opt: 319 z-score: 219.2 E(): 5.8e-05, 29.6% identity in 216 aa overlap 2 4 3 17 [Reporter]SCO7121 (5F21)_PCR/6611 [Gene]SCO7121/12043 NC_003888 SC4B10.22, possible secreted protein, len: 281 aa; similar to TR:Q9S1Z1 (EMBL:AL109747) Streptomyces coelicolor hypothetical 24.6 kDa protein SCJ21.16, 239 aa; fasta scores: opt: 989 z-score: 1075.6 E(): 0; 64.4% identity in 233 aa overlap and to Streptomyces coelicolor SC4B10.33, 239 aa; fasta scores: opt: 1034 z-score: 972.0 E(): 0; 67.0% identity in 233 aa overlap. Contains possible N-terminal region signal peptide sequence 2 4 3 16 [Reporter]SCO7577 (6B21)_PCR/6610 [Gene]SCO7577/12041 NC_003888 SC5F1.31, possible secreted hydrolase, len: 369 aa; similar to TR:P82594 (EMBL:AB023626) Streptomyces chartreusis alpha-L-arabinofuranosidase II precursor (EC 3.2.1.55), 328 aa; fasta scores: opt: 336 z-score: 382.3 E(): 9.2e-14; 27.8% identity in 302 aa overlap. Contains possible N-terminal region signal peptide sequence 4 1 19 17 [Reporter]SCO7786 (6H16)_PCR/1924 [Gene]SCO7786/3517 NC_003888 SC5E9.34c, unknown, len: 195 aa; regions of weak similarity to transposases eg. TR:Q9RBZ3 (EMBL:AF169828) from Pseudomonas syringae (pv. glycinea) (357 aa) fasta scores; opt: 158, z-score: 201.5, E(): 0.00093, 26.7% identity in 135 aa overlap. 4 1 19 16 [Reporter]SCO6178 (7D16)_PCR/1923 [Gene]SCO6178/3515 NC_003888 SC6C5.14c, possible deacetylase (putative secreted protein), len: 260 aa; similar to many e.g. SW:NODB_RHILT NodB, chitooligosaccharide deacetylase, nodulation protein B, from Rhizobium leguminosarum (215 aa) fasta scores; opt: 321, z-score: 420.9, E(): 3.7e-16, (35.0% identity in 197 aa overlap). Contains possible N-terminal region signal peptide sequence 2 4 3 15 [Reporter]SCO2124 (7N17)_PCR/6609 [Gene]SCO2124/12039 NC_003888 SC6E10.18, putative membrane protein, len: 205 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. The amino acid composition of this CDS is unusual, being Pro-rich and having a very acidic N-terminus. Contains probable coiled-coil from 1 to 29 (29 residues) (Max score: 1.455, probability 0.88). Contains possible hydrophobic membrane spanning regions 2 4 3 14 [Reporter]SCO2015 (8J17)_PCR/6608 [Gene]SCO2015/12037 NC_003888 SC7H2.29, possible secreted nucleotidase, len: 602 aa; similar to many e.g. SW:CN16_ECOLI periplasmic 2',3'-cyclic phosphodiesterase from Escherichia coli (647 aa) fasta scores; opt: 577, z-score: 618.9, E(): 3.8e-27, (32.3% identity in 595 aa overlap). Contains Pfam match to entry PF01009 5_nucleotidase, 5'-nucleotidase and Prosite match to PS00786 5'-nucleotidase signature 2. Also has possible N-terminal signal sequence. 2 4 3 13 [Reporter]SCO5743 (9F17)_PCR/6607 [Gene]SCO5743/12035 NC_003888 SC9A10.07, unknown, len: 246 aa; similar to e.g. Dictyostelium discoideum THY1_DICDI THY1 protein (260 aa), fasta scores; opt: 308 z-score: 443.0 E(): 2.1e-17, 35.4% identity in 198 aa overlap 2 4 3 12 [Reporter]SCO2447 (10B17)_PCR/6606 [Gene]SCO2447/12033 NC_003888 SCC24.18, unknown, len: 153 aa 2 4 3 11 [Reporter]SCO4699 (11N13)_PCR/6605 [Gene]SCO4699/12031 NC_003888 SCD31.24, possible Rhs protein, len: 927 aa; similar to C-terminal domain of SW:RHSB_ECOLI (EMBL:L02370) Escherichia coli RhsB protein precursor, 1411 aa; fasta scores: opt: 510 z-score: 568.8 E(): 3e-24; 26.4% identity in 954 aa overlap 4 1 19 10 [Reporter]SCO3097 (13L8)_PCR/1917 [Gene]SCO3097/3505 NC_003888 SCE41.06c, possible secreted protein, len: 244 aa; similar to TR:O86308 (EMBL:Z96935) Micrococcus luteus Rpf protein precursor, 220 aa; fasta scores: opt: 366 z-score: 357.9 E(): 2e-12; 39.7% identity in 234 aa overlap. Contains Pfam match to entry PF01476 PG_binding_2, Putative peptidoglycan binding domain and possible N-terminal region signal peptide sequence 2 4 3 10 [Reporter]SCO3513 (12J13)_PCR/6604 [Gene]SCO3513/12029 NC_003888 SCE134.14, possible secreted protein, len: 226 aa. Contains possible N-terminal region signal peptide sequence 2 4 3 9 [Reporter]SCO3920 (13F13)_PCR/6603 [Gene]SCO3920/12027 NC_003888 SCQ11.03c, cysA, probable cystathionine/methionine gamma-synthase/lyase, len: 392 aa; previously sequenced as SW:CYSA_STRCO (EMBL:U37580), cysA, S.coelicolor putative cystathionine gamma-lyase (392 aa). Similar to many e.g. SW:MEGL_PSEPU (EMBL:D88554), mdeA, Pseudomonas putida methionine gamma-lyase (398 aa), fasta scores; opt: 608 z-score: 653.6 E(): 4.5e-29, 36.9% identity in 404 aa overlap. Highly similar to TR:Q53668 (EMBL:X91393) Streptomyces antibioticus hypothetical protein found upstream of the abaB gene (232 aa) (87.1% identity in 232 aa overlap). Contains Pfam match to PF01053 Cys_Met_Meta_PP, Cys/Met metabolism PLP-dependent enzyme and PS00868 Cys/Met metabolism enzymes pyridoxal-phosphate attachment site 2 4 3 8 [Reporter]SCO1825 (14B13)_PCR/6602 [Gene]SCO1825/12025 NC_003888 SCI8.10, possible membrane protein, len: 212 aa. Contains a possible membrane spanning hydrophobic domain. 2 4 3 7 [Reporter]SCO3927 (15N9)_PCR/6601 [Gene]SCO3927/12023 NC_003888 SCQ11.10c, possible membrane protein, len: 513 aa; unknown function, probable CDS with Pro/Arg-rich N-terminus and 4x degenerate GGGHGA repeats from aa 58-81. Contains hydrophobic, possible membrane-spanning regions 2 4 3 6 [Reporter]SCO0890 (16J9)_PCR/6600 [Gene]SCO0890/12021 NC_003888 SCM1.23c, possible short chain oxidoreductase, len: 235 aa. Similar to several including: Bradyrhizobium japonicum SW:FIXR_BRAJA(EMBL:X06167) FixR protein involved in symbiotic nitrogen fixation (278 aa), fasta scores opt: 262 z-score: 283.4 E(): 1.9e-08 30.8% identity in 224 aa overlap and Streptomyces coelicolor TR:CAB56136(EMBL:AL117669) putative short chain oxidoreductase (299 aa), fasta scores opt: 290 z-score: 311.9 E(): 5e-10 38.6% identity in 233 aa overlap. Contains a Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 2 4 3 5 [Reporter]SCO0369 (17F9)_PCR/6599 [Gene]SCO0369/12019 NC_003888 SCF41.28c, putative secreted protein, len: 1085 aa. Contains a possible N-terminal signal peptide sequence 2 4 3 3 [Reporter]SCO4891 (19N5)_PCR/6597 [Gene]SCO4891/12017 NC_003888 2SCK8.17c, putative secreted protein, len: 128 aa. Contains possible N-terminal region signal peptide sequence 2 4 2 22 [Reporter]SCO5182 (1F1)_PCR/6594 [Gene]SCO5182/12015 NC_003888 2SC3B6.06, conserved hypothetical protein, len: 183 aa; similar to TR:Q9RX87 (EMBL:AE001902) Deinococcus radiodurans conserved hypothetical protein DR0428, 139 aa; fasta scores: opt: 187 z-score: 230.6 E(): 2.2e-05; 35.2% identity in 122 aa overlap 2 4 2 21 [Reporter]SCO4914 (2B1)_PCR/6593 [Gene]SCO4914/12013 NC_003888 SCK13.06c, probable deoxyribose-phosphate aldolase, len: 331 aa; similar to SW:DEOC_ECOLI (EMBL:X03224) Escherichia coli deoxyribose-phosphate aldolase (EC 4.1.2.4) DeoC, 259 aa; fasta scores: opt: 663 z-score: 743.7 E(): 0; 47.3% identity in 256 aa overlap. Contains Pfam match to entry PF01791 DeoC, Deoxyribose-phosphate aldolase 2 4 2 20 [Reporter]SCO0217 (2N21)_PCR/6592 [Gene]SCO0217/12011 NC_003888 SCJ12.29, narH2, probable nitrate reductase beta chain, len: 522 aa. Highly similar to many e.g. Streptomyces coelicolor TR:O86716 (EMBL; AL031515) putative nitrate reductase beta chain NarH SC5C7.19c (531 aa), fasta scores: opt: 2348 z-score: 2413.0 E(): opt: 2348 z-score: 2413.0 E(): 0 65.3% identity in 496 aa overlap and Bacillus subtilis SW:NARH_BACSU (EMBL; Z49884) nitrate reductase beta chain (EC 1.7.99.4) NarH (487 aa), fasta scores: opt: 2081 z-score: 2139.7 E(): 0 57.1% identity in 485 aa overlap. Contains a PS00190 Cytochrome c family heme-binding site signature, a Pfam match to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains and a Pfam match to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains. 2 4 2 19 [Reporter]SCO1391 (3J21)_PCR/6591 [Gene]SCO1391/12009 NC_003888 SC1A8A.11, phosphoenolpyruvate-protein phosphotransferase, len: 556 aa; similar to many e.g. SW:P08839 (PT1_ECOLI) PtsI, phosphoenolpyruvate-protein phosphotransferase from Escherichia coli (575 aa) fasta scores; opt: 473, z-score: 494.3, E(): 4.2e-20, 34.6% identity in 570 aa overlap. Contains Pfam match to entry PF00391 PEP-utilizers, PEP-utilizing enzymes, Prosite match to PS00370 PEP-utilizing enzymes phosphorylation site signature, Prosite match to PS00742 PEP-utilizing enzymes signature 2 and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 2 4 2 18 [Reporter]SCO5875 (4F21)_PCR/6590 [Gene]SCO5875/12007 NC_003888 SC2E9.16c, possible potassium uptake protein; len: 226 aa; similar to many homologs of trkA eg. Mycobacterium tuberculosis TR:O07194 (EMBL:Z96072) MTCY05A6.13 (220 aa), fasta scores; opt: 870 z-score: 1486.1 E(): 0, 58.8% identity in 216 aa overlap. Also similar to upstream ORF SC2E9.17c, trkA, (E(): 1.8e-05, 22.6% identity in 212 aa overlap) 2 4 2 17 [Reporter]SCO7116 (5B21)_PCR/6589 [Gene]SCO7116/12005 NC_003888 SC4B10.17c, conserved hypothetical protein, len: 247 aa; highly similar to TR:Q9RD73 (EMBL:AL136502) Streptomyces coelicolor hypothetical 27.0 kD protein SCF43.15c, 247 aa; fasta scores: opt: 1219 z-score: 1467.5 E(): 0; 71.6% identity in 250 aa overlap 2 4 2 16 [Reporter]SCO2170 (6N17)_PCR/6588 [Gene]SCO2170/12003 NC_003888 SC5F7.31, possible methyltransferase, len: 233 aa; similar to SW:SMTA_ECOLI methyltransferase from Escherichia coli (261 aa) fasta scores; opt: 301, z-score: 359.0, E(): 1.2e-12, (31.1% identity in 212 aa overlap). 2 4 2 15 [Reporter]SCO2120 (7J17)_PCR/6587 [Gene]SCO2120/12001 NC_003888 SC6E10.14c, probable two-component system regulator, len: 215 aa; similar to many e.g. SW:DEGU_BACBR (EMBL:M21658), DegU, Bacillus brevis transcriptional regulatory protein for the production of extracellular proteases (236 aa), fasta scores; opt: 443 z-score: 510.2 E(): 4.3e-21, 36.5% identity in 219 aa overlap. Similar to others from S.coelicolor e.g. SCH10.18C (EMBL:AL049754) S.coelicolor probable two-component system regulator (228 aa) (37.9% identity in 214 aa overlap). Contains probable helix-turn-helix motif at aa 169-190 (Score 1764, +5.19 SD). Contains Pfam matches to entry PF00072 response_reg, Response regulator receiver domain and to entry PF00196 GerE, Bacterial regulatory proteins, luxR family. Contains PS00622 Bacterial regulatory proteins, luxR family signature 2 3 4 4 [Reporter]SCO0636 (18F10)_PCR/4605 [Gene]SCO0636/8399 NC_003888 SCF56.20, probable ABC transporter ATP-binding protein, len: 544 aa; similar to TR:Q9X7M6 (EMBL:AL049587) Streptomyces coelicolor putative ABC transporter SC5F2A.03c, 544 aa; fasta scores: opt: 1673 z-score: 1706.2 E(): 0; 52.2% identity in 542 aa overlap and to SW:MSRA_STAEP (EMBL:X52085) Staphylococcus epidermidis erythromycin resistance ATP-binding protein MsrA, 488 aa; fasta scores: opt: 351 z-score: 362.8 E(): 7.9e-13; 28.6% identity in 514 aa overlap. Contains two Pfam matches to entry PF00005 ABC_tran, ABC transporter and two matches to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 3 4 3 [Reporter]SCP1.186 (19B10)_PCR/4604 [Gene]SCP1.186/8397 NC_003888 SCP1.186, conserved hypothetical protein, len: 282aa; similar to other hypothetical proteins from streptomycetes eg. SCE6.06 (TR:Q9KZT2 EMBL:AL353832) from Streptomyces coelicolor (281 aa) fasta scores; opt: 882, z-score: 1083.5, E(): 0, 47.9% identity in 280 aa overlap. Note possible alternative downstream translational start site. 2 3 4 2 [Reporter]SCP1.248c (20J6)_PCR/4603 [Gene]SCP1.248c/8395 NC_003888 SCP1.248c, unknown, len: 79aa; 2 3 3 22 [Reporter]SCO4541 (1J2)_PCR/4601 [Gene]SCO4541/8393 NC_003888 2SCD4.12c, possible membrane protein, len: 526 aa; N-terminal region identical to previously sequenced TR:Q9XD98 (EMBL:AF106004) Streptomyces coelicolor hypothetical 27.5 kDa protein, 249 aa and to TR:CAB82065 (EMBL:AL161803) Streptomyces coelicolor putative integral membrane protein SCD31.22, 604 aa; fasta scores: opt: 1299 z-score: 1315.4 E(): 0; 57.8% identity in 339 aa overlap. Contains possible coiled-coil region at approx. residues 108..198 and possible hydrophobic membrane spanning region 2 3 3 21 [Reporter]SCO0733 (2F2)_PCR/4600 [Gene]SCO0733/8391 NC_003888 3SC5B7.11c, hypothetical protein, len: 61 aa; similar to TR:Q9K3Z9 (EMBL:AL359949) Streptomyces coelicolor hypothetical 6.5 kDa protein 2SCG61.16, 59 aa; fasta scores: opt: 196 z-score: 304.8 E(): 1.9e-09; 59.3% identity in 54 aa overlap 2 3 3 20 [Reporter]SCO4647 (3B2)_PCR/4599 [Gene]SCO4647/8389 NC_003888 SCD82.18, nusG, transcription antitermination protein, len: 300aa; identical to previously sequenced SW:NUSG_STRCO (EMBL:D17465) Streptomyces coelicolor transcription antitermination protein NusG. Contains match to Prosite entry PS01014 Transcription termination factor nusG signature. Contains also possible coiled-coil region at aprox. residues 56..75 2 3 3 19 [Reporter]SCO1405 (3N22)_PCR/4598 [Gene]SCO1405/8387 NC_003888 SC1A8A.25c, possible heat shock protein (hsp90-family), len: 615aa; similar to many eg. TR:O43642 (EMBL:AF043254) heat shock protein 75 from Homo sapiens (Human) (649 aa) fasta scores; opt: 364, z-score: 416.0, E(): 9.7e-16, 25.5% identity in 639 aa overlap. Contains two Pfam matches to entry PF00183 HSP90, Hsp90 protein. 2 3 3 18 [Reporter]SCO6233 (4J22)_PCR/4597 [Gene]SCO6233/8385 NC_003888 SC2H4.15, probable transcriptional regulator, len: 342 aa; similar to many members of the lacI family e.g. CCPA_BACME glucose-resistance amylase regulator (Bacillus megaterium) (332 aa), fasta scores; opt: 448 z-score: 548.6 E(): 2.6e-23, 29.8% identity in 336 aa overlap. Contains helix-turn-helix motif from aa 9-30 (Score 1778, +5.24 SD) and Pfam matches to entry PF00356 lacI, Bacterial regulatory proteins, lacI family, score 30.40, E-value 4.7e-07 and to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family, score 44.00, E-value 3.4e-09 2 3 3 17 [Reporter]SCO7130 (5F22)_PCR/4596 [Gene]SCO7130/8383 NC_003888 SC4B10.31, hypothetical protein, len: 262 aa; similar to TR:O53404 (EMBL:AL021897) Mycobacterium tuberculosis hypothetical 28.9 kDa protein MTV017.09, 254 aa; fasta scores: opt: 820 z-score: 1018.9 E(): 0; 49.0% identity in 249 aa overlap 2 3 3 16 [Reporter]SCO7585 (6B22)_PCR/4595 [Gene]SCO7585/8381 NC_003888 SC5F1.39c, probable merR-family transcriptional regulator, len: 132 aa; similar to SW:SOXR_PSEAE (EMBL:X95517) Pseudomonas aeruginosa SoxR protein, 156 aa; fasta scores: opt: 205 z-score: 264.7 E(): 3.3e-07; 36.8% identity in 117 aa overlap. Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and match to Prosite entry PS00552 Bacterial regulatory proteins, merR family signature and possible helix-turn-helix at residues 12..33 (+4.37 SD) 2 3 3 15 [Reporter]SCO4407 (7N18)_PCR/4594 [Gene]SCO4407/8379 NC_003888 SC6F11.05, hypothetical protein, len: 257 aa; partially similar to TR:Q9X8P9 (EMBL:AL049754) Streptomyces coelicolor hypothetical 17.1 kDa protein SCH10.10c, 152 aa; fasta scores: opt: 314 z-score: 367.2 E(): 6e-13 40.4% identity in 141 aa overlap 1 4 15 10 [Reporter]SCO3018 (13I1)_PCR/6364 [Gene]SCO3018/11597 NC_003888 SCE33.20, possible regulatory protein, len: 329 aa; similar to TR:O69660 (EMBL:AL022121) Mycobacterium tuberculosis putative regulatory protein MTV025.040, 358 aa; fasta scores: opt: 1523 z-score: 1708.1 E(): 0; 70.9% identity in 330 aa overlap and to TR:CAA22801 (EMBL:AL035212) Streptomyces coelicolor putative regulatory protein, SC9B2.21c, 332 aa; fasta scores: opt: 956 z-score: 937.8 E(): 0; 50.7% identity in 306 aa overlap. Contains Pfam match to entry PF01078 Mg_chelatase, Magnesium chelatase, subunit ChlI 2 3 3 14 [Reporter]SCO7589 (8J18)_PCR/4593 [Gene]SCO7589/8377 NC_003888 SC7H9.01c, unknown (fragment), len: >158,SC5F1.43c, unknown (fragment), len: >161 aa 1 4 15 9 [Reporter]SCO5022 (14E1)_PCR/6363 [Gene]SCO5022/11595 NC_003888 SCK15.24, possible lipoprotein, len: 220 aa; similar to Streptomyces coelicolor SCK15.23, 221 aa; fasta scores: opt: 736 z-score: 703.5 E(): 1.3e-33; 54.3% identity in 221 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 2 3 3 13 [Reporter]SCO7146 (9F18)_PCR/4592 [Gene]SCO7146/8375 NC_003888 SC9A4.08, possible regulatory protein, len: 116 aa; similar to TR:Q9X8Y1 (EMBL:AL078610) Streptomyces coelicolor putative transcriptional regulator SCH35.25, 102 aa; fasta scores: opt: 144 z-score: 203.2 E(): 0.00075; 37.9% identity in 87 aa overlap. Contains Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family 1 4 15 8 [Reporter]SCO1716 (15A1)_PCR/6362 [Gene]SCO1716/11593 NC_003888 SCI11.05, possible gntR-family transcriptional regulator, len: 249 aa; weakly similar to many trancriptional regulators e.g. SW:FARR_ECOLI (EMBL:X15790), FarR, Escherichia coli fatty acyl responsive regulator (240 aa), fasta scores; opt: 213 z-score: 265.9 E(): 1.8e-07, 25.0% identity in 232 aa overlap. Similar to others from S.coelicolor e.g. SCE39.14c (EMBL:AL049573) S.coelicolor possible gntR-family regulator (243 aa) (29.5% identity in 224 aa overlap). Contains probable helix-turn-helix motif at aa 42-63 (Score 1296, +3.60 SD). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 2 3 3 12 [Reporter]SCO2455 (10B18)_PCR/4591 [Gene]SCO2455/8373 NC_003888 SCC24.26c, possible spermidine synthase, len: 531 aa; similar to TR:Q9X8S2 (EMBL:AL049754) Streptomyces coelicolor putative spermidine synthase SCH10.33c, 554 aa; fasta scores: opt: 1413 z-score: 1545.7 E(): 0; 47.9% identity in 528 aa overlap and C-terminal region similar to SW:SPEE_ECOLI (EMBL:J02804) Escherichia coli spermidine synthase (EC 2.5.1.16) SpeE, 287 aa; fasta scores: opt: 420 z-score: 465.4 E(): 1.7e-18; 31.4% identity in 271 aa overlap. Contains Pfam match in C-terminal region to entry PF01564 Spermine_synth, Spermine/spermidine synthase and N-terminal region contains also possible hydrophobic membrane spanning regions 1 4 15 7 [Reporter]SCO0815 (15M21)_PCR/6361 [Gene]SCO0815/11591 NC_003888 SCF43A.05, conserved hypothetical protein, len: 250 aa; unknown function, similar to other hypothetical proteins e.g. TR:O07020 (EMBL:Z94043) Bacillus subtilis hypothetical protein (238 aa), fasta scores; opt: 720 z-score: 887.0 E(): 0, 46.1% identity in 245 aa overlap. Also similar to the C-terminal half of many Fe-S proteins e.g. SW:GLPC_ECOLI (EMBL:M20938), glpC, Escherichia coli anaerobic glycerol-3-phosphate dehydrogenase subunit C (396 aa) (26.7% identity in 255 aa overlap) 2 3 3 11 [Reporter]SCO4501 (11N14)_PCR/4590 [Gene]SCO4501/8371 NC_003888 SCD35.08c, probable bifunctional protein (ketoreductase/3-oxoacyl-[acyl-carrier protein] reductase), len: 430 aa; highly similar to TR:O53665 (EMBL:AL021929) Mycobacterium tuberculosis 3-oxoacyl-[acyl-carrier protein] reductase MTV034.08c, 454 aa; fasta scores: opt: 1227 z-score: 1186.5 E(): 0; 50.8% identity in 455 aa overlap, N-terminal region similar to TR:O86480 (EMBL:AJ007932) Streptomyces argillaceus ketoreductase MtmTII, 253 aa; blastp results: Identities = 54/204 (26%), Positives = 87/204 (42%) and C-terminal region to SW:FABG_ECOLI (EMBL:M84991) Escherichia coli 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) (3-ketoacyl-acyl carrier protein reductase) FabG, 244 aa; fasta scores: opt: 538 z-score: 529.0 E(): 4.9e-22; 41.0% identity in 249 aa overlap. Contains Pfam matches to entries PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus,PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 2 3 3 10 [Reporter]SCO3286 (12J14)_PCR/4589 [Gene]SCO3286/8369 NC_003888 SCE15.03, putative secreted protein, len: 677 aa; weak similarity to SW:YQIK_ECOLI hypothetical protein from Escherichia coli (553 aa) fasta scores; opt: 542, z-score: 501.2, E(): 1.3e-20, (27.0% identity in 504 aa overlap). Contains possible N-terminal region signal peptide sequence also contains a highly hidrophilic central part of the protein 1 4 15 5 [Reporter]SCO3642 (17E21)_PCR/6359 [Gene]SCO3642/11587 NC_003888 SCH10.20c, hypothetical membrane protein, len: 420 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains several hydrophobic, probable membrane-spanning regions. Contains PS00133 Zinc carboxypeptidases, zinc-binding region 2 signature 2 3 3 9 [Reporter]SCO3416 (13F14)_PCR/4588 [Gene]SCO3416/8367 NC_003888 SCE9.23, gad, probable glutamate decarboxylase, len: 475 aa; similar to many e.g. SW:DCEA_ECOLI (EMBL:M84024), gadA, Escherichia coli glutamate decarboxylase alpha (466 aa), fasta scores; opt: 1433 z-score: 1665.2 E(): 0, 50.0% identity in 426 aa overlap. Contains Pfam match to entry PF00282 pyridoxal_deC, Pyridoxal-dependent decarboxylase conserved domain, score 276.70, E-value 1.1e-79 1 4 15 4 [Reporter]SCO3666 (18A21)_PCR/6358 [Gene]SCO3666/11585 NC_003888 SCH44.06c, possible regulatory protein, len: 394 aa; similar to TR:Q56084 (EMBL:D63904) Streptomyces thermoviolaceus ESA36 (erythropoiesis-stimulating activity), 344 aa; fasta scores: opt: 380 z-score: 432.8 E(): 1.2e-16; 31.1% identity in 286 aa overlapand to Streptomyces colicolor SCH44.04, 336 aa; fasta scores: opt: 952 z-score: 855.5 E(): 0; 48.1% identity in 320 aa overlap and to SCH44.05, 327 aa; fasta scores: opt: 849 z-score: 764.1 E(): 0; 45.2% identity in 323 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and possible helix-turn-helix motif at residues 328..349 (+3.19 SD) 2 3 3 8 [Reporter]SCO4872 (14B14)_PCR/4587 [Gene]SCO4872/8365 NC_003888 SCK20.13c, unknown, len: 338 aa 1 4 15 3 [Reporter]SCP1.155 (19M17)_PCR/6357 [Gene]SCP1.155/11583 NC_003888 SCP1.155, unknown, len: 318aa; 2 3 3 7 [Reporter]SCO0193 (15N10)_PCR/4586 [Gene]SCO0193/8363 NC_003888 SCJ12.05c, possible DNA-binding regulator, len:334 aa. Some similarity to Myxococcus xanthus TR:Q50900 (EMBL:Z21955) regulatory protein (299 aa), fasta scores opt: 121 z-score: 141.6 E(): 1.6 32.4% identity in 281 aa overlap and Myxococcus xanthus TR:Q50899 (EMBL:Z21955) regulatory protein (288 aa), fasta scores opt: 118 z-score: 138.5 E() 2.4 26.9% identity in 279 aa overlap. Contains a possible helix-turn-helix motif between aa's 23..44. 1 4 15 2 [Reporter]SCO7505 (20E17)_PCR/6356 [Gene]SCO7505/11581 NC_003888 SCBAC17A6.38, possible integral membrane binding-protein-dependent transport protein, len: 299aa; similar to many eg. TR:Q9L0B4 (EMBL:AL163003) putative sugar transporter integral membrane protein from Streptomyces coelicolor (313 aa) fasta scores: opt: 905, Z-score: 1049.4, 50.000% identity (51.799% ungapped) in 288 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component; Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and multiple hydrophobic possible membrane spanning regions. 2 3 3 6 [Reporter]SCO0745 (16J10)_PCR/4585 [Gene]SCO0745/8361 NC_003888 SCF81.04c, possible tetR-family transcriptional regulator, len: 219 aa; similar to various Streptomyces transcriptional regulators, e.g. TR:Q9ZBK0 (EMBL:AL035161) Streptomyces coelicolor putative transcriptional regulator SC9C7.10c, 272 aa; fasta scores: opt: 189 z-score: 243.3 E(): 3.5e-06; 29.8% identity in 218 aa overlap. Contains match to Pfam entry PF00440 tetR, Bacterial regulatory proteins, tetR family 2 3 3 5 [Reporter]SCO3667 (17F10)_PCR/4584 [Gene]SCO3667/8359 NC_003888 SCH44.07c, probable secreted solute-binding protein, len: 372 aa; similar to TR:O50503 (EMBL:AL009199) Streptomyces coelicolor possible solute-binding protein SC7B7.06, 371 aa; fasta scores: opt: 1441 z-score: 1539.7 E(): 0; 60.6% identity in 376 aa overlap and to SW:XYLF_ECOLI (EMBL:U00039) Escherichia coli D-xylose-binding periplasmic protein precursor XylF, 330 aa; fasta scores: opt: 559 z-score: 602.9 E(): 4.1e-26; 34.3% identity in 318 aa overlap. Contains Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide sequence 1 4 14 21 [Reporter]SCO2614 (2A13)_PCR/6353 [Gene]SCO2614/11577 NC_003888 SCC88.25c, fpgS, folylpolyglutamate synthase, len: 444 aa; identical to previously sequenced TR:O08416 (EMBL:Y13070) Streptomyces coelicolor folylpolyglutamate synthase (EC 6.3.2.17), 444 aa and similar to SW:FOLC_BACSU (EMBL:L04520) Bacillus subtilis folylpolyglutamate synthase (EC 6.3.2.17), FolC, 430 aa; fasta scoreS: opt: 622 z-score: 688.7 E(): 6.1e-31; 32.0% identity in 438 aa overlap. Contains Pfam match to entry PF01225 Mur_ligase, Mur ligase family and matches to Prosite entries PS00398 Site-specific recombinases signature 2 and PS01012 Folylpolyglutamate synthase signature 2 2 3 3 4 [Reporter]SCO0870 (18B10)_PCR/4583 [Gene]SCO0870/8357 NC_003888 SCM1.03c, probable two-component system response regulator, len: 149 aa. Highly similar to the response regulator receiver domains of putative two-component regulatory proteins including: Synechocystis sp. (strain PCC 6803) TR:P72781(EMBL:D90900) regulatory components of sensory transduction system (282 aa), fasta scores opt: 229 z-score: 290.6 E(): 7.7e-09 31.8% identity in 129 aa overlap and Methanobacterium thermoautotrophicum TR:O26545(EMBL:AE000828) sensory transduction regulatory protein (145 aa), fasta scores opt: 422 z-score: 531.6 E(): 2.9e-22 47.2% identity in 142 aa overlap. Contains a Pfam match to entry PF00072 response_reg, Response regulator receiver domain 1 4 14 20 [Reporter]SCO5898 (3M9)_PCR/6352 [Gene]SCO5898/11575 NC_003888 SC10A5.03, redF, probable membrane protein, len: 226 aa. Contains possibe hydrophobic membrane spanning regions 2 3 3 3 [Reporter]SCP1.128 (19N6)_PCR/4582 [Gene]SCP1.128/8355 NC_003888 SCP1.128, unknown, len: 265aa; Contains a TTA encoded leucine at residue 43, possible target for bldA regulation. 1 4 14 19 [Reporter]SCO6936 (4I9)_PCR/6351 [Gene]SCO6936/11573 NC_003888 SC1G8.08, unknown, len: 217 aa. Weakly similar to Streptomyces coelicolor TR:CAB56672 (EMBL:AL121596) hypothetical protein, SCF51A.20(252 aa), fasta scores opt: 111 z-score: 140.7 E(): 2.2 27.9% identity in 190 aa overlap. 2 3 3 2 [Reporter]SCP1.09c (20F6)_PCR/4581 [Gene]SCP1.09c/8353 NC_003888 SCP1.09c, unknown, len: 221aa; 1 4 14 18 [Reporter]SCO6403 (5E9)_PCR/6350 [Gene]SCO6403/11571 NC_003888 SC3C8.22c, unknown, len: 126 aa 2 3 2 22 [Reporter]SCO5190 (1F2)_PCR/4579 [Gene]SCO5190/8351 NC_003888 2SC3B6.14, possible DNA-binding protein, len: 122 aa; similar to TR:Q9X952 (EMBL:AJ239087) Streptomyces coelicolor hypothetical 9.7 kD protein WblE, 85 aa; fasta scores: opt: 183 z-score: 243.0 E(): 4.6e-06; 40.5% identity in 74 aa overlap and to TR:Q9S426 (EMBL:AF164439) Mycobacterium smegmatis WhmD, 129 aa; fasta scores: opt: 161 z-score: 213.2 E(): 0.00021; 29.0% identity in 93 aa overlap. Contains Pfam match to entry PF02178 AT_hook, DNA binding domain with preference for A/T rich regions and match to Prosite entry PS00354 HMG-I and HMG-Y DNA-binding domain (A+T-hook) 2 3 2 21 [Reporter]SCO0729 (2B2)_PCR/4578 [Gene]SCO0729/8349 NC_003888 3SC5B7.07, conserved hypothetical protein, len: 140 aa; similar to TR:O27449 (EMBL:AE000902) Methanobacterium thermoautotrophicum conserved protein MTH1398, 133 aa; fasta scores: opt: 122 z-score: 167.4 E(): 0.085; 26.7% identity in 116 aa overlap 1 4 14 16 [Reporter]SCO5433 (7M5)_PCR/6348 [Gene]SCO5433/11567 NC_003888 SC6A11.09, possible AfsR-like regulator, len: 816 aa; similar to others from Streptomyces spp. e.g. SW:P25941 (AFSR_STRCO) regulatory protein AfsR from Streptomyces coelicolor (993 aa) fasta scores: opt: 490, z-score: 458.0, E(): 4.2e-18, 33.7% identity in 656 aa overlap. Note that this protein contains a Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) but no helix-turn-helix motifs 2 3 2 20 [Reporter]SCO2612 (2N22)_PCR/4577 [Gene]SCO2612/8347 NC_003888 SCC88.23c, ndk, nucleoside diphosphate kinase, 137 aa; identical to previously sequenced SW:NDK_STRCO (EMBL:X95643) Streptomyces coelicolor nucleoside diphosphate kinase (EC 2.7.4.6) Ndk, 137 aa. Contains Pfam match to entry PF00334 NDK, Nucleoside diphosphate kinases and match to Prosite entry PS00469 Nucleoside diphosphate kinases active site 1 4 14 15 [Reporter]SCO7241 (8I5)_PCR/6347 [Gene]SCO7241/11565 NC_003888 SC7A12.08, possible oxidoreductase, len: 540 aa. Similar to many including: Streptomyces griseus SW:CRTI_STRGR(EMBL:L37405) phytoene dehydrogenase (EC 1.3.-.-) (507 aa), fasta scores opt: 175 z-score: 191.7 E(): 0.0033 27.0% identity in 529 aa overlap and Mycobacterium tuberculosis SW:Y897_MYCTU(EMBL:Z73101) hypothetical 56.2 kda protein (535 aa), fasta scores opt: 1633 z-score: 1765.0 E():0 52.0% identity in 527 aa overlap. Contains a Pfam match to entry PF02032 Phytoene_dh, Phytoene dehydrogenase related enzyme. 2 3 2 19 [Reporter]SCO1401 (3J22)_PCR/4576 [Gene]SCO1401/8345 NC_003888 SC1A8A.21c, cvnB4, unknown, len: 144 aa; similar to others from Streptomyces coelicolor and Mycobacterium tuberculosis e.g. TR:Q9X835 (EMBL:AL049727) hypothetical protein from Streptomyces coelicolor (139 aa) fasta scores; opt: 408, z-score: 500.5, E(): 1.9e-20, 47.6% identity in 143 aa overlap. Local genetic arrangement also seems to be conserved for the Streptomyces coelicolor paralogues 1 4 14 14 [Reporter]SCO7724 (9E5)_PCR/6346 [Gene]SCO7724/11563 NC_003888 SC8D11.15c, hypothetical protein, len: 471 aa; similar to TR:Q9WX25 (EMBL:AL079345) Streptomyces coelicolor putative membrane protein SCE68.06c, 464 aa; fasta scores: opt: 428 z-score: 361.4 E(): 1.4e-12; 40.4% identity in 478 aa overlap 2 3 2 18 [Reporter]SCO6188 (4F22)_PCR/4575 [Gene]SCO6188/8343 NC_003888 SC2G5.09, possible transferase, len: 566aa; C-terminal region similar to SW:RFAC_SALTY lipopolysaccharide heptosyltransferase-1 (317aa) blast scores; Sum P(2)= 1.3e-09, Identities= 46 /150 (30%), Positives = 70 /150 (46%). 1 4 14 13 [Reporter]SCO5130 (10A5)_PCR/6345 [Gene]SCO5130/11561 NC_003888 SC9E12.15, possible ABC transporter integral membrane protein, len: 246 aa; similar to TR:CAB88184 (EMBL:AL352972) Streptomyces coelicolor putative ABC transporter integral membrane protein SCC30.14, 238 aa; fasta scores: opt: 516 z-score: 611.2 E(): 1.4e-26; 35.9% identity in 237 aa overlap. Contains possible hydrophobic membrane spanning regions 2 3 2 17 [Reporter]SCO7125 (5B22)_PCR/4574 [Gene]SCO7125/8341 NC_003888 SC4B10.26c, conserved hypothetical protein, len: 246 aa; highly similar to TR:Q9S1U9 (EMBL:AL109950) Streptomyces coelicolor hypothetical 25.2 kDa protein SCJ4.24c, 242 aa; fasta scores: opt: 643 z-score: 729.3 E(): 0; 45.1% identity in 244 aa overlap and to Streptomyces coelicolor SC4B10.09c, 248 aa; fasta scores: opt: 779 z-score: 733.5 E(): 0; 51.6% identity in 244 aa overlap 2 3 2 16 [Reporter]SCO7292 (6N18)_PCR/4573 [Gene]SCO7292/8339 NC_003888 SC5F8.02c, possible threonine dehydratase, len: 319 aa. Similar to many including: Escherichia coli SW:THD2_ECOLI(EMBL:M21312) threonine dehydratase catabolic (EC 4.2.1.16) TdcB (329 aa), fasta scores opt: 609 z-score: 676.6 E(): 3.2e-30 39.4% identity in 312 aa overlap. Contains a Prosite hit to Serine/threonine dehydratases pyridoxal-phosphate attachment site and a Pfam match to entry PF00291 S_T_dehydratase, Pyridoxal-phosphate dependent enzyme. 1 4 14 11 [Reporter]SCO4486 (12I1)_PCR/6343 [Gene]SCO4486/11557 NC_003888 SCD69.06, hypothetical protein, len: 135 aa; similar to TR:Q9Z549 (EMBL:AL035212) Streptomyces coelicolor putative quinone protein SC9B2.10c, 130 aa; fasta scores: opt: 191 z-score: 257.8 E(): 6.8e-07; 34.6% identity in 127 aa overlap and to TR:Q98IY9 (EMBL:AP002999) Rhizobium loti (Mesorhizobium loti) MLR2185 protein, 133 aa; fasta scores: opt: 522 Z-score: 639.6 E(): 5.4e-28; 64.122% identity in 131 aa overlap 2 3 2 15 [Reporter]SCO2128 (7J18)_PCR/4572 [Gene]SCO2128/8337 NC_003888 SC6G10.01c, partial CDS, unknown, len: >40aa;,SC6E10.22c, hypothetical protein, len: 390 aa; unknown function, similar to the N-terminal half of anion-transporter ATPases e.g. TR:O50593 (EMBL:AB004659), arsA, Acidiphilium multivorum plasmid pKW301 arsenite activated ATPase of translocating ATPase (583 aa), fasta scores; opt: 190 z-score: 208.0 E(): 0.00029, 27.3% identity in 267 aa overlap. Previously partially sequenced as SW:YGL1_STRCO (EMBL:X65932) Streptomyces coelicolor hypothetical protein (341 aa), identical to this sequence 1 4 14 10 [Reporter]SCO2854 (13E1)_PCR/6342 [Gene]SCO2854/11555 NC_003888 SCE20.28, possible transmembrane efflux protein, len: 477 aa. Similar to many including: Mycobacterium smegmatis TR:Q50392(EMBL:U40487) proton antiporter efflux pump (504 aa), fasta scores opt: 626 z-score: 586.9 E(): 2.6e-25 32.1% identity in 471 aa overlap and Streptomyces coelicolor TR:Q9ZBW5(EMBL:AL034443) putative integral membrane efflux protein SC4B5.03C (504 aa), fasta scores opt: 729 z-score: 681.9 E(): 1.3e-30 36.6% identity in 435 aa overlap. Contains a Prosite hit to PS00216 Sugar transport proteins signature 1 and multiple possible membrane spanning hydrophobic domains. 2 3 2 14 [Reporter]SCO6866 (8F18)_PCR/4571 [Gene]SCO6866/8335 NC_003888 SC7F9.18c, unknown, len: 396 aa. Weakly similar to Deinococcus radiodurans TR:AAF11162 (EMBL:AE002004) conserved hypothetical protein (470 aa), fasta scores opt: 145 z-score: 156.5 E():0.26 25.1% identity in 407 aa overlap. 1 4 14 9 [Reporter]SCO0846 (14A1)_PCR/6341 [Gene]SCO0846/11553 NC_003888 SCF43A.36, hypothetical protein, len: 266 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 2 3 2 13 [Reporter]SCO7142 (9B18)_PCR/4570 [Gene]SCO7142/8333 NC_003888 SC9A4.04, hypothetical protein, len: 133 aa; similar to SW:YODB_BACSU (EMBL:AF006665) Bacillus subtilis hypothetical 12.8 kDa protein in OdhA-CtpA intergenic region YodB, 112 aa; fasta scores: opt: 258 z-score: 328.5 E(): 7.9e-11; 44.0% identity in 91 aa overlap. Contains Pfam match to entry PF01638 DUF24, Protein of unknown function DUF24 1 4 14 8 [Reporter]SCO1899 (14M21)_PCR/6340 [Gene]SCO1899/11551 NC_003888 SCI7.17, possible integral membrane sugar transport protein, len: 314aa; similar to many eg. TR:O30832 (EMBL:AF018073) sorbitol/mannitol transport inner membrane protein from Rhodobacter sphaeroides (290 aa) fasta scores; opt: 722, z-score: 829.0, E(): 0, (40.6% identity in 276 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible membrane spanning hydrophobic regions. 2 3 2 12 [Reporter]SCO2330 (10N14)_PCR/4569 [Gene]SCO2330/8331 NC_003888 SCC53.21, possible isochorismatase, len: 196 aa; region similar to many eg. SW:P15048 (ENTB_ECOLI) isochorismatase (EC 3.3.2.1) from Escherichia coli (285 aa) fasta scores; opt: 123, z-score: 154.2, E(): 0.41, 28.9% identity in 114 aa overlap. Also similar to TR:P96654 (EMBL:AB001488) hypothetical protein from Bacillus subtilis (180 aa) fasta scores; opt: 358, z-score: 433.4, E(): 1.1e-16, 36.7% identity in 177 aa overlap. Contains Pfam match to entry PF00857 Isochorismatase, Isochorismatase family. 2 3 2 11 [Reporter]SCO4497 (11J14)_PCR/4568 [Gene]SCO4497/8329 NC_003888 SCD35.04, possible membrane protein, len: 297 aa. Contains possible hydrophobic membrane spanning regions 1 4 14 6 [Reporter]SCO0694 (16E21)_PCR/6338 [Gene]SCO0694/11547 NC_003888 SCF42.04, unknown, len: 62 aa. 2 3 2 10 [Reporter]SCO4245 (12F14)_PCR/4567 [Gene]SCO4245/8327 NC_003888 SCD8A.18c, hypothetical protein, len: 150 aa; similar to TR:Q988W8 (EMBL:AP003009) MLR6561 protein from Rhizobium loti (Mesorhizobium loti) (136 aa) fasta scores; opt: 360, Z-score: 445.9, E(): 3.3e-17, 44.882% identity (47.107% ungapped) in 127 aa overlap. Also similar to TR:Q9ZXK9 (EMBL:AB008550) Pseudomonas aeruginosa phage phi CTX ORF17, 114 aa; fasta scores: opt: 172 z-score: 230.4 E(): 2.1e-05; 30.2% identity in 106 aa overlap 1 4 14 5 [Reporter]SCO2942 (17A21)_PCR/6337 [Gene]SCO2942/11545 NC_003888 SCE59.01c, possible oxidoreductase (partial), len: >116 aa; similar to N-terminal region of TR:Q9ZBN9 (EMBL:AL034492) Streptomyces coelicolor putative oxidoreductase subunit SC6C5.06, 296 aa; fasta scores: opt: 223 z-score: 268.2 E(): 1.6e-07; 37.6% identity in 93 aa overlap and to TR:P95637 (EMBL:U65440) Rhodopseudomonas palustris 4-hydroxybenzoyl-CoA reductase HbaD subunit, 327 aa; fasta scores: opt: 193 z-score: 233.2 E(): 1.4e-05; 42.6% identity in 94 aa overlap,SCE56.06, possible oxidoreductase (partial), len: >221 aa; similar of TR:Q9ZBN9 (EMBL:AL034492) Streptomyces coelicolor putative oxidoreductase subunit SC6C5.06, 296 aa; fasta scores: opt: 393 z-score: 443.2 E(): 2.8e-17; 34.7% identity in 222 aa overlap and to TR:P95637 (EMBL:U65440) Rhodopseudomonas palustris 4-hydroxybenzoyl-CoA reductase HbaD subunit, 327 aa; fasta scores: opt: 159 z-score: 184.7 E(): 0.0071; 32.1% identity in 184 aa overlap 2 3 2 9 [Reporter]SCO3225 (13B14)_PCR/4566 [Gene]SCO3225/8325 NC_003888 SCE8.18, absA1, two component sensor kinase, len: 571aa; sequenced previously therefore identical to TR:Q53893 (EMBL:U51332) AbsA1, predicted sensor histidine kinase which acts as part of a two component signal transduction system in the global regulation of antibiotic synthesis in Streptomyces coelicolor (571 aa) fasta scores; opt: 3782, z-score: 3585.7, E(): 0, (100.0% identity in 571 aa overlap). 1 4 14 4 [Reporter]SCO4035 (18M17)_PCR/6336 [Gene]SCO4035/11543 NC_003888 2SC10A7.39c, sigF, RNA polymerase sigma factor (fragment), len: >31 aa; identical to previously sequenced SW:RPOF_STRCO (EMBL:L11648) Streptomyces coelicolor RNA polymerase sigma factor SigF or RpoX, 278 aa,2SCD60.01c, sigF, RNA polymerase sigma factor, len: 287 aa; identical to previously sequenced SW:RPOF_STRCO (EMBL:L11648) Streptomyces coelicolor RNA polymerase sigma factor sigma-F SigF or RpoX, 287 aa. Contains Pfam match to entry PF00140 sigma70, Sigma-70 factor and match to Prosite entry PS00715 Sigma-70 factors family signature 1. Also contains possible helix-turn-helix motif at residues 252..273 (+6.22 SD) 2 3 2 8 [Reporter]SCO0750 (14N10)_PCR/4565 [Gene]SCO0750/8323 NC_003888 SCF81.09c, unknown, len: 59 aa 1 4 14 3 [Reporter]SCO4986 (19I17)_PCR/6335 [Gene]SCO4986/11541 NC_003888 2SCK36.09c, probable D-aminoacylase, len: 536 aa; similar to SW:NDAD_ALCXX (EMBL:S80683) Alcaligenes xylosoxydans D-aminoacylase (EC 3.5.1.81) Dan, 483 aa; fasta scores: opt: 370 Z-score: 413.6 bits: 86.1 E(): 2e-15; 33.647% identity in 532 aa overlap 2 3 2 7 [Reporter]SCO5015 (15J10)_PCR/4564 [Gene]SCO5015/8321 NC_003888 SCK15.17, possible secreted protein, len: 203 aa; similar to many other membrane proteins, e.g. TR:Q9S6B8 (EMBL:AF115336) Pseudomonas fluorescens major outer membrane protein OprF, 343 aa; fasta scores: opt: 247 z-score: 285.4 E(): 2e-08; 34.3% identity in 181 aa overlap. Contains Pfam match to entry PF00691 OmpA, OmpA family and possible N-terminal region signal peptide sequence 2 3 2 6 [Reporter]SCO0415 (16F10)_PCR/4563 [Gene]SCO0415/8319 NC_003888 SCF51.14, possible integral membrane protein, len: 120 aa. Contains possible hydrophobic membrane spanning regions 1 4 13 22 [Reporter]SCO1278 (1A13)_PCR/6332 [Gene]SCO1278/11537 NC_003888 2SCG18.25, possible ATP/GTP-binding protein, len: 525 aa; similar to TR:O27682 (EMBL:AE000923) Methanobacterium thermoautotrophicum ABC transporter MTH1645, 561 aa; fasta scores: opt: 540 z-score: 600.5 E(): 5.8e-26; 30.1% identity in 372 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 2 3 2 5 [Reporter]SCO1592 (17B10)_PCR/4562 [Gene]SCO1592/8317 NC_003888 SCI35.14c, hypothetical protein, len: 180 aa; similar to other hypothetical proteins containing a mutT domain e.g. YQKG_BACSU (185 aa), fasta scores; opt: 192 z-score: 280.5 E(): 2.2e-08, 29.2% identity in 168 aa overlap. Contains PS00893 mutT domain signature, Pfam match to entry PF00293 mutT, Bacterial mutT protein, score 28.70, E-value 1e-06, and TTA Leu codon: possible target for action of bldA 1 4 13 21 [Reporter]SCO5739 (2M9)_PCR/6331 [Gene]SCO5739/11535 NC_003888 SC9A10.03, dapB, dihydrodipicolinate reductase, len: 250 aa; highly similar to many e.g. DAPB_CORGL dihydrodipicolinate reductase (EC 1.3.1.26) (248 aa), fasta scores; opt: 946 z-score: 1333.7 E(): 0, 56.1% identity in 246 aa overlap. Contains PS01298 Dihydrodipicolinate reductase signature 2 3 2 4 [Reporter]SCO0356 (18N6)_PCR/4561 [Gene]SCO0356/8315 NC_003888 SCF41.15, probable oxidoreductase, len: 263 aa. Shares a high level of sequence similarity with Comamonas testosteroni TR:P94681 (EMBL:U32622) toluenesulfonate zinc-independent alcohol dehydrogenase (252 aa), fasta scores opt: 506 z-score: 549.4 E(): 3e-23 39.8% identity in 244 aa overlap and Streptomyces coelicolor TR:O88068 (EMBL:AL031541) putative dehydrogenase SCI35.33C (260 aa), fasta scores opt: 500 z-score: 542.8 E(): 7e-23 36.4% identity in 253 aa overlap. Contains a Pfam match to entry PF00106 adh_short, short chain dehydrogenase, Pfam match to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and a PS00061 Short-chain dehydrogenases/reductases family signature 1 4 13 20 [Reporter]SCO3023 (3I9)_PCR/6330 [Gene]SCO3023/11533 NC_003888 SCE34.04c, sahH, adenosylhomocysteinase, len: 485 aa; highly similar to SW:SAHH_TRIVA (EMBL:U40872) Trichomonas vaginalis adenosylhomocysteinase (EC 3.3.1.1) SahH, 486 aa; fasta scores: opt: 2114 z-score: 2420.8 E(): 0; 65.2% identity in 477 aa overlap. Contains Pfam match to entry PF00670 AdoHcyase, S-adenosyl-L-homocysteine hydrolase and matches to Prosite entries PS00738 S-adenosyl-L-homocysteine hydrolase signature 1 and PS00739 S-adenosyl-L-homocysteine hydrolase signature 2 2 3 2 3 [Reporter]SCP1.114 (19J6)_PCR/4560 [Gene]SCP1.114/8313 NC_003888 SCP1.114, unknown, len: 152aa; 1 4 13 19 [Reporter]SCO5540 (4E9)_PCR/6329 [Gene]SCO5540/11531 NC_003888 SC1C2.21c, cvnA2, unknown, len: 1111 aa; contains two possible transmembrane domains at around 100 and 400 aa, a Gln-rich region at around 850 aa and a probable coiled-coil domain from 297 to 327. Overall 35.6% identical to upstream gene SC1C2.25c, but with much higher similarity between aa 477 and 719 2 3 2 2 [Reporter]SCP1.219 (20B6)_PCR/4559 [Gene]SCP1.219/8311 NC_003888 SCP1.219, unknown, len: 173aa; similar to others from Streptomyces coelicolor eg. TR:Q9Z552 (EMBL:AL035212) hypothetical protein (162 aa) blastp scores; Identities = 60/162 (37%), Positives = 80/162 (49%) and SW:P14706 (YM2_STRCO) mini-circle hypothetical 13.3 kDa protein (122 aa) blastp scores; Identities = 38/118 (32%), Positives = 60/118 (50%). 2 3 1 22 [Reporter]SCO5186 (1B2)_PCR/4557 [Gene]SCO5186/8309 NC_003888 2SC3B6.10, conserved hypothetical protein, len: 318 aa; similar to TR:O53345 (EMBL:AL021646) Mycobacterium tuberculosis hypothetical 33.8 kD protein MTV014.43c, 313 aa; fasta scores: opt: 334 z-score: 389.2 E(): 3.3e-14; 33.3% identity in 318 aa overlap 1 4 13 17 [Reporter]SCO5813 (6M5)_PCR/6327 [Gene]SCO5813/11527 NC_003888 SC5B8.03, unknown, len: 212 aa; highly Proline rich in N-terminus 2 3 1 21 [Reporter]SCO4937 (1N22)_PCR/4556 [Gene]SCO4937/8307 NC_003888 SCK13.29c, hypothetical protein, len: 172 aa; similar to TR:CAC04235 (EMBL:AL391515) Streptomyces coelicolor conserved hypothetical protein SC4B10.30c, 156 aa; fasta scores: opt: 215 z-score: 274.7 E(): 9.2e-08; 30.9% identity in 139 aa overlap 1 4 13 16 [Reporter]SCO5429 (7I5)_PCR/6326 [Gene]SCO5429/11525 NC_003888 SC6A11.05c, possible integral membrane transport protein, len: 313 aa; similar to many proposed transport proteins e.g. TRNEW:CAB55533(EMBL:AL117322) putative integral membrane transport protein from Streptomyces coelicolor (317 aa) fasta scores; opt: 561, z-score: 649.2, E(): 9.4e-29, 34.4% identity in 314 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. and possible membrane-spanning hydrophobic regions 2 3 1 20 [Reporter]SCO4950 (2J22)_PCR/4555 [Gene]SCO4950/8305 NC_003888 2SCK31.10, narI3, nitrate reductase gamma chain, len: 237 aa; highly similar to SW:NARI_BACSU (EMBL:Z49884) Bacillus subtilis nitrate reductase gamma chain (EC 1.7.99.4) NarI, 223 aa; fasta scores: opt: 792 z-score: 991.9 E(): 0; 49.6% identity in 234 aa overlap and to TR:Q9RI29 (EMBL:109989) Streptomyces coelicolor putative nitrate reductase delta chain NarI2 SCJ12.31, 256 aa; fasta scores: opt: 813 z-score: 891.7 E(): 0; 54.3% identity in 230 aa overlap. Contains possible hydrophobic membrane spanning regions 1 4 13 14 [Reporter]SCO7720 (9A5)_PCR/6324 [Gene]SCO7720/11523 NC_003888 SC8D11.11, unknown, len: 368 aa 2 3 1 19 [Reporter]SCO7394 (3F22)_PCR/4554 [Gene]SCO7394/8303 NC_003888 SC10G8.22, possible integral membrane protein, len: 312 aa. Similar to several proteins of undefined function e.g. Deinococcus radiodurans TR:AAF11939(EMBL:AE002070) Conserved hypothetical protein (304 aa), fasta scores opt: 227 z-score: 259.6 E(): 4.8e-07 29.4% identity in 310 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence. 1 4 13 13 [Reporter]SCO6251 (10M1)_PCR/6323 [Gene]SCO6251/11521 NC_003888 SCAH10.16, possible reductase, len: 185 aa; similar to TR:Q9ZGD0 (EMBL:AF080235) Streptomyces cyanogenus reductase homologue LanO, len: 193 aa; fasta scores: opt: 398 z-score: 505.3 E(): 9e-21; 41.0% identity in 188 aa overlap 2 3 1 18 [Reporter]SCO6184 (4B22)_PCR/4553 [Gene]SCO6184/8301 NC_003888 SC2G5.05, possible transferase, len: 312aa, C-terminal region similar to C-terminal from many eg. TR:Q47594 (EMBL:D43637) mannosyl transferase B from Escherichia coli fasta scores; opt: 149, z-score: 178.9, E(): 0.011, (32.0% identity in 150 aa overlap). 1 4 13 11 [Reporter]SCO4449 (12E1)_PCR/6321 [Gene]SCO4449/11517 NC_003888 SCD6.27c, probable hydrolase, len: 299 aa; similar to TR:O87275 (EMBL:AF015628) Staphylococcus cohnii streptogramin B lactonase VgbB, 295 aa; fasta scores: opt: 855 z-score: 887.0 E(): 0; 44.5% identity in 283 aa overlap. Contains 5x DNA degenerate repeats:G/R)(T/A)(G/R)RR(P/H)VVH 1 4 13 10 [Reporter]SCO2850 (13A1)_PCR/6320 [Gene]SCO2850/11515 NC_003888 SCE20.24c, possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, len: 285 aa. Similar to many including: Mycobacterium tuberculosis TR:O53242 (EMBL:AL021287) possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (239 aa), fasta scores opt: 544 z-score: 651.1 E(): 7e-29 40.3% identity in 221 aa overlap and similar in parts to the C-terminus of Escherichia coli SW:HPCE_ECOLI (EMBL:X75028) 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-) (405 aa), fasta scores opt: 467 z-score: 556.6 E(): 1.3e-23 40.2% identity in 214 aa overlap. Also similar to Streptomyces coelicolor TR:CAB55708 (EMBL:AL117387) putative 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase SCF41.05 (292 aa), fasta scores opt: 1142 z-score: 1213.4 E():0 61.5% identity in 286 aa overlap. Contains a Pfam match to entry PF01557 FAA_hydrolase, Fumarylacetoacetate (FAA) hydrolase family. 1 4 13 9 [Reporter]SCO0631 (13M21)_PCR/6319 [Gene]SCO0631/11513 NC_003888 SCF56.15c, unknown, len: 60 aa 1 4 13 8 [Reporter]SCO3430 (14I21)_PCR/6318 [Gene]SCO3430/11511 NC_003888 SCE9.37, rpsN, probable 30S ribosomal protein S14, len: 101 aa; similar to many e.g. SW:RS14_ECOLI (EMBL:X01563), rpsN, Escherichia coli 30S ribosomal protein S14 (100 aa), fasta scores; opt: 299 z-score: 377.0 E(): 1.1e-13, 44.0% identity in 100 aa overlap. Contains Pfam match to entry PF00253 Ribosomal_S14, Ribosomal protein S14, score 103.10, E-value 1.5e-30 1 4 13 7 [Reporter]SCO1711 (15E21)_PCR/6317 [Gene]SCO1711/11509 NC_003888 SCI30A.32c, possible dehydrogenase, len: 747aa; similar to many e.g. TR:O53648 (EMBL:AL021928) hypothetical protein from Mycobacterium tuberculosis (762 aa) fasta scores; opt: 617, z-score: 625.8, E(): 1.6e-27, (29.9% identity in 762 aa overlap) and SW:FDHF_ECOLI formate dehydrogenase from Escherichia coli (715 aa) fasta scores; opt: 466, z-score: 473.3, E(): 4.9e-19, (24.0% identity in 716 aa overlap). 1 4 13 5 [Reporter]SCO0744 (17M17)_PCR/6315 [Gene]SCO0744/11507 NC_003888 SCF81.03, unknown, len: 121 aa; similar to TR:Q98BX4 (EMBL:AP003006) MLL5389 protein from Rhizobium loti (Mesorhizobium loti) (124 aa) fasta scores; opt: 363, Z-score: 452.3, E(): 1.5e-17, 43.750% identity (43.750% ungapped) in 112 aa overlap. 1 4 13 4 [Reporter]SCO6094 (18I17)_PCR/6314 [Gene]SCO6094/11505 NC_003888 SCBAC1A6.18c, probable transport system integral membrane protein, len: 258 aa; similar to SW:SSUC_BACSU (EMBL:Z93102) Bacillus subtilis putative aliphatic sulfonates transport permease protein SsuC, 276 aa; fasta scores: opt: 378 z-score: 422.1 E(): 6.2e-16; 28.5% identity in 263 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible hydrophobic membrane spanning regions 1 4 13 3 [Reporter]SCO4064 (19E17)_PCR/6313 [Gene]SCO4064/11503 NC_003888 2SCD60.30c, unknown, len: 87 aa 1 4 13 2 [Reporter]SCO5072 (20M13)_PCR/6312 [Gene]SCO5072/11501 NC_003888 SCBAC20F6.15, ORF1 hydroxylacyl-CoA dehydrogenase, len: 307 aa; identical to previously sequenced TR:Q53926 (EMBL:X62373) Streptomyces coelicolor hydroxylacyl-CoA dehydrogenase ORF1, 307 aa. Contains Pfam matches to entries PF02737 3HCDH_N, 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain and PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain. Contains possible hydrophobic membrane spanning region 1 3 14 21 [Reporter]SCO0561 (2A14)_PCR/4338 [Gene]SCO0561/7899 NC_003888 SCF73.08c, furS, Fe regulatory protein, len: 151 aa; identical to TR:CAB38251 (EMBL:AJ132989) S. coelicolor Fe regulatory protein (151 aa), highly similar to e.g. FUR_MYCTU ferric uptake regulation protein (147 aa), fasta scores; opt: 613 z-score: 742.2 E(): 0, 64.8% identity in 142 aa overlap. Contains Pfam match to entry PF01475 FUR, Ferric uptake regulator family 1 3 14 20 [Reporter]SCO5908 (3M10)_PCR/4337 [Gene]SCO5908/7897 NC_003888 SC10A5.13, unknown, len: 64 aa 1 3 14 19 [Reporter]SCO6944 (4I10)_PCR/4336 [Gene]SCO6944/7895 NC_003888 SC1G8.16c, conserved hypothetical protein, len: 633 aa. Highly similar to several proteins of undefined function including: Streptomyces coelicolor TR:CAB52841 (EMBL:AL109848) SCI51.17 (612 aa), fasta scores opt: 2658 z-score: 3108.3 E():0 64.4% identity in 590 aa overlap and Schizosaccharomyces pombe (Fission yeast) SW:YAY3_SCHPO (EMBL:Z69380) SPAC4H3.03C (649 aa), fasta scores opt: 943 z-score: 1101.2 E():0 32.8% identity in 640 aa overlap. 1 3 14 18 [Reporter]SCO5445 (5E10)_PCR/4335 [Gene]SCO5445/7893 NC_003888 SC3D11.02c, hypothetical protein, len: 188aa; previously sequenced as TR:Q9X945 (EMBL:AJ001205) and predicted as a 134 aa protein. Also similar to TR:O06198 (EMBL:Z95387) hypothetical protein from Mycobacterium tuberculosis (166 aa) fasta scores; opt: 407, z-score: 496.4, E(): 3.1e-20, 44.0% identity in 159 aa overlap 1 3 14 17 [Reporter]SCO5784 (6A10)_PCR/4334 [Gene]SCO5784/7891 NC_003888 SC4H2.05, probable two-component sensor protein, len: 358 aa; similar to many e.g. DEGS_BACSU P13799 sensor protein DegS (ec 2.7.3.-), Bacillus subtilis (385 aa), fasta scores; opt: 317 z-score: 334.6 E(): 2e-11, 31.3% identity in 227 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 14 16 [Reporter]SCO6754 (7M6)_PCR/4333 [Gene]SCO6754/7889 NC_003888 SC6A5.03, possible glycerol dehydrogenase, len: 340aa; similar to many eg. SW:GLDA_PSEPU glycerol dehydrogenase, GldA, from Pseudomonas putida (365 aa) fasta scores; opt: 261, z-score: 303.7, E(): 1.3e-09, (27.9% identity in 308 aa overlap). 1 3 14 15 [Reporter]SCO7250 (8I6)_PCR/4332 [Gene]SCO7250/7887 NC_003888 SC7A12.17c, unknown, len: 639 aa. Highly similar to another hypothetical protein: Streptomyces coelicolor TR:Q9S2M7(EMBL:AL109661) SC6E10.10 (943 aa), fasta scores opt: 1245 z-score: 1258.0 E(): 0 42.5% identity in 664 aa overlap. Contains a Pfam match to entry PF01510 Amidase_2, N-acetylmuramoyl-L-alanine amidase. 1 3 14 14 [Reporter]SCO7732 (9E6)_PCR/4331 [Gene]SCO7732/7885 NC_003888 SC8D11.23, unknown, len: 128 aa 1 3 14 13 [Reporter]SCO5713 (10A6)_PCR/4330 [Gene]SCO5713/7883 NC_003888 SC9F2.03, possible peptide transport ATP-binding protein, len: 393aa; similar to many (confirmed and proposed) eg. SW:OPPD_ECOLI oligopeptide transport ATP-binding protein from Escherichia coli (337 aa) fasta scores; opt: 870, z-score: 693.2, E(): 2.7e-31, (44.1% identity in 311 aa overlap).Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also similar to the gene immediately downstream (SC9F2.04) suggesting a local duplication event fasta scores; opt: 722, z-score: 477.4, E(): 9.4e-22, (42.8% identity in 327 aa overlap). Also contains Pfam match to entry PF00005 ABC_tran, ABC transporter, score 197.30, E-value 2.3e-55. 1 3 14 11 [Reporter]SCO4116 (12I2)_PCR/4328 [Gene]SCO4116/7881 NC_003888 SCD72A.02, possible AfsR-like regulatory protein, len: 1114 aa; similar to many from actinomycetes e.g. TR:CAB61705 (EMBL:AL133220) putative multi-domain regulatory protein from Streptomyces coelicolor (1334 aa) fasta scores; opt: 1324, z-score: 1364.5, E(): 0, 41.1% identity in 1357 aa overlap and SW:P25941 (AFSR_STRCO) regulatory protein AfsR from Streptomyces coelicolor (993 aa) fasta scores; opt: 533, z-score: 548.6, E(): 4.3e-23, 29.3% identity in 1014 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 14 10 [Reporter]SCO2863 (13E2)_PCR/4327 [Gene]SCO2863/7879 NC_003888 SCE20.37, possible helicase, len: 945 aa. Weakly similar to Saccharomyces cerevisiae (Baker's yeast) TR:Q06683(EMBL:U32517) DEAD box family helicase family protein (689 aa), fasta scores opt: 455 z-score: 511.5 E(): 4.1e-21 27.2% identity in 357 aa overlap. Also weakly similar to several bacteriophage proteins including: Lactococcus bacteriophage (isolate 7-9) SW:V51K_BPL79 (EMBL:M36388) 51.5 KD protein which may play a role in either regulating bacteriophage replication or bacteriophage specific genes (452 aa), fasta scores opt: 407 z-score: 460.0 E(): 3.1e-18 27.4% identity in 328 aa overlap. Contains Prosite hit to 2xPS00017 ATP/GTP-binding site motif A (P-loop) and a Pfam match to entry PF00271 helicase_C, Helicases conserved C-terminal domain. 1 3 14 9 [Reporter]SCO0399 (14A2)_PCR/4326 [Gene]SCO0399/7877 NC_003888 SCF62.25, possible membrane protein, len: 407 aa. Weakly similar to a several e.g. Erwinia amylovora SW: AMSJ_ERWAM (EMBL; X77921) amylovoran biosynthesis protein AmsJ (415 aa), fasta scores opt: 107 z-score: 129.1 E(): 7.8 21.3% identity in 423 aa overlap and Escherichia coli SW:WCAK_ECOLI (EMBL; U38473) colanic acid biosynthesis protein WcaK (426 aa), fasta scores opt: 111 z-score: 133.6 E():4.3 20.1% identity in 398 aa overlap. Contains a possible N-terminal signal sequence and a membrane spanning hydrophobic domain. Also contains a TTA leucine codon, possible target for bldA regulation and a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 14 8 [Reporter]SCO1845 (14M22)_PCR/4325 [Gene]SCO1845/7875 NC_003888 SCI8.30, possible low-affinity phosphate transport protein, len: 423 aa. Highly similar to many possible transporters, including: Streptomyces halstedii SW: PITH_STRHA (EMBL: L05390) putative low-affinity inorganic phosphate transporter (fragment) (>213 aa), fasta scores opt: 505 z-score: 517.8 E(): 1.8e-21 45.9% identity in 194 aa overlap and Mycobacterium tuberculosis TR:O06411 (EMBL: Z95558) hypothetical 42.7 KD protein (417 aa), fasta scores opt: 1286 z-score: 1295.4 E():0 50.8% identity in 421 aa overlap. Contains a Pfam match to entry PF01384 PHO4, Phosphate transporter family. Also contains a possible N-terminal signal sequence and multiple possible membrane spanning hydrophobic domains. 1 3 14 7 [Reporter]SCO0544 (15I22)_PCR/4324 [Gene]SCO0544/7873 NC_003888 SCF11.24, hypothetical secreted protein, len: 510 aa; similar to various hypothetical proteins, e.g. SW:YM26_MYCTU (EMBL:Z70692) Mycobacterium tuberculosis hypothetical 56.3 KD protein RV2226, 513 aa; fasta scores: opt: 867 z-score: 952.2 E(): 0; 39.0% identity in 515 aa overlap. Contains probable N-terminal signal sequence 1 3 14 6 [Reporter]SCO1886 (16E22)_PCR/4323 [Gene]SCO1886/7871 NC_003888 SCI7.04c, possible integral membrane transport protein, len: 298aa; similar to many eg. SW:YURM_BACSU hypothetical ABC transporter from Bacillus subtilis (300 aa) fasta scores; opt: 582, z-score: 650.2, E(): 6.9e-29, (31.7% identity in 293 aa overlap) and TR:O87858 (EMBL:AL031013) putative transmembrane sugar transport protein from Streptomyces coelicolor (279 aa) fasta scores; opt: 540, z-score: 604.3, E(): 2.5e-26, (34.7% identity in 277 aa overlap). Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and Prosite match to PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Also contains possible membrane spanning hydrophobic regions. 1 3 14 5 [Reporter]SCO3559 (17A22)_PCR/4322 [Gene]SCO3559/7869 NC_003888 SCH5.22c, possible oxidoreductase, len: 274aa; similar to TR:O69630 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (256 aa) fasta scores; opt: 289, z-score: 335.7, E(): 2.1e-11, (39.3% identity in 270 aa overlap). Also contains a region of similarity to many fatty acid desaturases from plants eg. SW:STAD_RICCO Stearoyl-acyl-carrier-protein desaturase from Ricinus communis (castor bean) (396 aa) fasta scores; opt: 112, z-score: 133.1, E(): 4.1, (25.3% identity in 146 aa overlap). 1 3 14 4 [Reporter]SCP1.126 (18M18)_PCR/4321 [Gene]SCP1.126/7867 NC_003888 SCP1.126, unknown, len: 78aa; 1 3 14 3 [Reporter]SCP1.264 (19I18)_PCR/4320 [Gene]SCP1.264/7865 NC_003888 SCP1.264, unknown, len: 460aa; 1 3 14 2 [Reporter]SCO3992 (20A18)_PCR/4319 [Gene]SCO3992/7863 NC_003888 SCBAC25E3.29c, unknown, doubtful CDS, len: 96 aa: no significant database matches. 1 3 13 22 [Reporter]SCO1286 (1A14)_PCR/4317 [Gene]SCO1286/7861 NC_003888 2SCG18.33, probable oxidoreductase, len: 374 aa; similar to TR:P96072 (EMBL:U76606) Streptomyces viridifaciens isobutylamine N-hydroxylase (EC 1.14.-.-) (IBAH) VlmH, 378 aa; fasta scores: opt: 1020 z-score: 1177.8 E(): 0; 45.9% identity in 379 aa overlap 1 3 13 21 [Reporter]SCO2554 (2M10)_PCR/4316 [Gene]SCO2554/7859 NC_003888 SCC77.21c, dnaJ2, DnaJ protein, len: 378 aa. Highly similar to many including: Escherichia coli SW:DNAJ_ECOLI(EMBL:M12565) DnaJ protein (375 aa), fasta scores opt: 1057 z-score: 1055.3 E():0 45.0% identity in 378 aa overlap and Streptomyces albus g TR:O52164(EMBL:AF025656) DnaJ protein (379 aa), fasta scores opt: 2401 z-score: 2385.8 E():0 92.6% identity in 378 aa overlap. Contains a Prosite hit to PS00636 Nt-dnaJ domain signature and Pfam matches to entries PF00226 DnaJ, PF00684 DnaJ_CXXCXGXG and PF01556 DnaJ_C. 1 3 13 20 [Reporter]SCO5903 (3I10)_PCR/4315 [Gene]SCO5903/7857 NC_003888 SC10A5.08, questionable ORF, possible pseudogene fragment, len: 125; contains segment of strong similarity to part of S. coelicolor regulator TR:Q53894 (EMBL:U51332) AbsA2 (222 aa), fasta scores; opt: 110 z-score: 210.7 E(): 0.00 016, 47.1% identity in 70 aa overlap 1 3 13 19 [Reporter]SCO5551 (4E10)_PCR/4314 [Gene]SCO5551/7855 NC_003888 SC1C2.32, unknown, len: 190 aa 1 3 13 18 [Reporter]SCO6407 (5A10)_PCR/4313 [Gene]SCO6407/7853 NC_003888 SC3C8.26, possible gamma-glutamyltranspeptidase (putative secreted protein), len: 603 aa; similar to many e.g. GGT_BACSU gamma-glutamyltranspeptidase precursor (EC 2.3.2.2) (587 aa), fasta scores; opt: 705 z-score: 863.9 E(): 0, 36.9% identity in 591 aa overlap, and to PAC1_PSESV cephalosporin acylase I (EC 3.5.1.-) (557 aa), fasta scores; opt: 377 z-score: 819.8 E(): 0, 34.1% identity in 569 aa overlap. Contains probable N-terminal signal sequence 1 3 13 17 [Reporter]SCO5824 (6M6)_PCR/4312 [Gene]SCO5824/7851 NC_003888 SC5B8.14, probable two-component sensor, len: 384 aa; N-termius has six possible transmembrane domains, C-terminus is similar to histidine kinase domain of many two-component sensors e.g. NARQ_ECOLI P27896 nitrate/nitrite sensor (566 aa) fasta scores; opt: 220 z-score: 227.4 E(): 1.8e-0 5, 32.3% identity in 198 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 19 7 [Reporter]SCO3322 (16P4)_PCR/1914 [Gene]SCO3322/3499 NC_003888 SCE68.20, putative membrane protein, len: 298 aa; unknown function, similar to TR:O33611 (EMBL:AB004855) Streptomyces cyaneus hypothetical protein (277 aa), fasta scores; opt: 463 z-score: 536.3 E(): 1.5e-22, 34.2% identity in 269 aa overlap. C-terminus similar to other hypothetical proteins e.g. SW:YV32_MYCTU (EMBL:Z77162) from Mycobacterium tuberculosis (373 aa) (57.8% identity in 256 aa overlap) and SCH5.21 (EMBL:AL035636) S.coelicolor hypothetical protein (268 aa) (36.3% identity in 267 aa overlap). Contains possible hydrophobic membrane spanning region at C-terminal domain 1 3 13 16 [Reporter]SCO5437 (7I6)_PCR/4311 [Gene]SCO5437/7849 NC_003888 SC6A11.13, possible MerR-family transcriptional regulator, len: 132 aa; similar to many e.g. TR:Q9X5V4 (EMBL:AF127795) heavy metal regulator HmrR from Rhizobium leguminosarum (biovar viciae) (129 aa) fasta scores; opt: 228, z-score: 295.1, E(): 5e-09, 35.7% identity in 129 aa overlap. Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and possible helix-turn-helix motif score 1747 (+5.14 SD) in ID at residue 1 1 3 13 15 [Reporter]SCO4753 (8E6)_PCR/4310 [Gene]SCO4753/7847 NC_003888 SC6G4.31, unknown, len: 90 aa 1 3 13 14 [Reporter]SCO7728 (9A6)_PCR/4309 [Gene]SCO7728/7845 NC_003888 SC8D11.19, conserved hypothetical protein, len: 102 aa; similar to TR:Q9RTC1 (EMBL:AE002024) Deinococcus radiodurans conserved hypothetical protein DR1844, 93 aa; fasta scores: opt: 232 z-score: 318.9 E(): 3.2e-10; 45.8% identity in 83 aa overlap 1 3 13 13 [Reporter]SCO6259 (10M2)_PCR/4308 [Gene]SCO6259/7843 NC_003888 SCAH10.24, probable ABC sugar transport ATP binding protein, len: 263 aa; similar to TR:O68121 (EMBL:AF010496) Rhodobacter capsulatus ribose transport ATP-binding protein, 305 aa; fasta scores: opt: 612 z-score: 706.1 E(): 5.9e-32; 45.6% identity in 217 aa overlap, to SW:RBSA_ECOLI (EMBL:M13169) Escherichia coli, ribose transport ATP-binding protein RbsA, 501 aa; fasta scores: opt: 509 z-score: 585.6 E(): 3e-25; 31.5% identity in 254 aa overlap and to TR:CAA15787 (EMBL:SC7B7) Streptomyces coelicolor St7B7.07, 260 aa; fasta scores: opt: 592 z-score: 521.2 E(): 1e-23; 43.1% identity in 246 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 3 13 12 [Reporter]SCO2353 (11I2)_PCR/4307 [Gene]SCO2353/7841 NC_003888 SCC8A.11c, unknown, len: 213aa; weakly similar to others eg. TR:Q9XAQ0 (EMBL:AL078618) putative acetyltransferase from Streptomyces coelicolor (187 aa) fasta scores; opt: 133, z-score: 173.2, E(): 0.035, 27.2% identity in 162 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 4 1 19 2 [Reporter]SCO5101 (20H24)_PCR/1909 [Gene]SCO5101/3489 NC_003888 SCBAC28G1.27, conserved hypothetical protein, len: 63 aa; similar to TR:Q9HTF6 (EMBL:AE004953) Pseudomonas aeruginosa hypothetical protein PA5408, 58 aa; fasta scores: opt: 146 Z-score: 225.2 bits: 45.1 E(): 6.2e-05; 45.652% identity in 46 aa overlap 1 3 13 11 [Reporter]SCO4457 (12E2)_PCR/4306 [Gene]SCO4457/7839 NC_003888 SCD6.35c, possible integral membrane protein, len: 333 aa. Contains possible hydrophobic membrane spanning regions 1 3 13 10 [Reporter]SCO2858 (13A2)_PCR/4305 [Gene]SCO2858/7837 NC_003888 SCE20.32c, unknown, len: 85 aa. 1 3 13 9 [Reporter]SCO3546 (13M22)_PCR/4304 [Gene]SCO3546/7835 NC_003888 SCH5.09c, possible small lipoprotein, len: 196aa; Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site and probable N-terminal signal sequence. Contains probable coiled-coil from 104 to 141 (38 residues), max score: 1.630, (probability 1.00). 1 3 13 8 [Reporter]SCO0465 (14I22)_PCR/4303 [Gene]SCO0465/7833 NC_003888 SCF76.05c, non-heme chloroperoxidase (chloride peroxidase), len: 276 aa. Identical to Streptomyces lividans SW:PRXC_STRLI (EMBL; U02635) non-heme chloroperoxidase (EC 1.11.1.10) (chloride peroxidase)(CPO-L) (chloroperoxidase L) (275 aa), fasta scores opt: 1866 z-score: 2162.6 E():0 99.3% identity in 275 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 1 3 13 7 [Reporter]SCO0429 (15E22)_PCR/4302 [Gene]SCO0429/7831 NC_003888 SCF51A.07c, possible oxidoreductase, len: 315 aa. Weakly similar to many eukaryotic oxidoreductases e.g. Hordeum vulgare (Barley) TR:Q42837 (EMBL; Z48360) aldose reductase (EC 1.1.1.21) (aldehyde reductase) (320 aa), fasta scores opt: 236 z-score: 263.3 E(): 2.6e-07 27.0% identity in 296 aa overlap and many other putative oxidoreductases e.g. Streptomyces coelicolor TR:CAB42953 (EMBL:AL049863) putative oxidoreductase SC5H1.28C (305 aa), fasta scores opt: 563 z-score: 617.0 E(): 5.2e-27 39.7% identity in 305 aa overlap 4 1 18 17 [Reporter]SCO7779 (6D16)_PCR/1902 [Gene]SCO7779/3479 NC_003888 SC5E9.27c, possible oxidoreductase, len: 244aa; similar to many eg. SW:P16544 (ACT3_STRCO) putative ketoacyl reductase from Streptomyces coelicolor (261 aa) fasta scores; opt: 236, z-score: 270.7, E(): 1.3e-07, 31.3% identity in 233 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 1 3 13 6 [Reporter]SCO0462 (16A22)_PCR/4301 [Gene]SCO0462/7829 NC_003888 SCF76.02c, possible oxidoreductase, len: 305 aa. Similar to many hypothetical proteins e.g. Pyrococcus horikoshii SW:APBA_PYRHO (EMBL; AP000006) probable 2-dehydropantoate 2-reductase (EC 1.1.1.169) (ketopantoate reductase) (KPA reductase) (301 aa), fasta scores opt: 359 z-score: 419.9 E(): 4.9e-16 26.5% identity in 291 aa overlap and Streptomyces coelicolor TR:Q9ZBV6 (EMBL:AL034443) putative oxidoreductase SC4B5.12C (323 aa), fasta scores opt: 233 z-score: 274.7 E(): 6.1e-08 32.0% identity in 322 aa overlap. 1 3 13 5 [Reporter]SCO0897 (17M18)_PCR/4300 [Gene]SCO0897/7827 NC_003888 SCM1.30c, unknown, len: 197 aa; similar to the plasmid borne TR:CAC48467 (EMBL:AL603642) Rhizobium meliloti (Sinorhizobium meliloti) hypothetical protein SMB20067, 173 aa; fasta scores: opt: 483 Z-score: 502.3 E(): 2.4e-20; 51.370% identity) in 146 aa overlap 1 3 13 4 [Reporter]SCP1.141 (18I18)_PCR/4299 [Gene]SCP1.141/7825 NC_003888 SCP1.141, unknown, len: 297aa; 1 3 13 3 [Reporter]SCO4906 (19E18)_PCR/4298 [Gene]SCO4906/7823 NC_003888 2SCK8.32c, afsQ2, sensor kinase protein, len: 535 aa; identical to previously sequenced SW:AFQ2_STRCO (EMBL:D10654) Streptomyces coelicolor sensor protein AfsQ2, 535 aa. Contains 3x Pfam matches to entries PF00672 DUF5, HAMP domain, PF00512 signal, Histidine kinase and PF02518 HATPase_c, Histidine kinase. Contains possible hydrophobic membrane spanning region 1 3 13 2 [Reporter]SCO5087 (20M14)_PCR/4297 [Gene]SCO5087/7821 NC_003888 SCBAC28G1.13, actIORF1, actinorhodin polyketide beta-ketoacyl synthase alpha subunit, len: 467 aa; identical to C-terminal region of SW:KASA_STRCO (EMBL:X63449) Streptomyces coelicolor actinorhodin polyketide putative beta-ketoacyl synthase 1 ActI ORF1, 424 aa. Previously sequenced start codon at residue 44 of this sequence. Also similar to TR:CAB45606 (EMBL:AL079356) Streptomyces coelicolor polyketide beta-ketoacyl synthase alpha (WhiE locus) SC6G9.15, 424 aa; fasta scores: opt: 1706 Z-score: 1660.2 bits: 316.4 E(): 9.5e-87; 61.871% identity in 417 aa overlap. Contains Pfam matches to entries PF00109 ketoacyl-synt, Beta-ketoacyl synthase, N-terminal domain and PF02801 ketoacyl-synt_C, Beta-ketoacyl synthase, C-terminal domain and match to Prosite entry PS00606 Beta-ketoacyl synthases active site 4 1 18 12 [Reporter]SCO4401 (11P8)_PCR/1897 [Gene]SCO4401/3469 NC_003888 SCD10.33c, possible lipoprotein, len: 438 aa; N-terminal domain similar to N-terminal domain of TR:O68027 (EMBL:AF010496) Rhodobacter capsulatus potential Leu/Ile/Val-binding protein precursor, 400 aa; fasta scores: opt: 151 z-score: 166.0 E(): 0.09; 29.8% identity in 131 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 1 3 12 22 [Reporter]SCO1257 (1M10)_PCR/4295 [Gene]SCO1257/7819 NC_003888 2SCG18.04c, possible ABC transport system integral membrane protein, len: 241 aa; similar to TR:CAB94565 (EMBL:AL359152) Streptomyces coelicolor putative ABC transport system integral membrane protein 2SC6G5.38c, 258 aa; fasta scores: opt: 513 z-score: 578.5 E(): 9.7e-25; 39.8% identity in 256 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 12 21 [Reporter]SCO4856 (2I10)_PCR/4294 [Gene]SCO4856/7817 NC_003888 SC5G8.24c, dhsA, probable succinate dehydrogenase flavoprotein subunit, len: 584aa; strongly similar to many eg. SW:P10444 (DHSA_ECOLI) succinate dehydrogenase flavoprotein subunit from Escherichia coli (588 aa) fasta scores; opt: 1842, z-score: 2076.6, E(): 0, 48.4% identity in 591 aa overlap. Contains Pfam match to entry PF00890 FAD_binding_2, FAD binding domain and Prosite match to PS00504 Fumarate reductase / succinate dehydrogenase FAD-binding site. 1 3 12 20 [Reporter]SCO4813 (3E10)_PCR/4293 [Gene]SCO4813/7815 NC_003888 SCD63A.24, purN, phosphoribosylglycinamide formyltransferase, len: 215 aa; highly similar to TR:AAF05726 (EMBL:AF191543) Mycobacterium paratuberculosis phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) PurN, 209 aa; fasta scores: opt: 708 z-score: 829.2 E(): 0; 57.6% identity in 203 aa overlap. Contains Pfam match to entry PF00551 formyl_transf, Formyl transferase and match to Prosite entry PS00373 Phosphoribosylglycinamide formyltransferase active site 1 3 12 19 [Reporter]SCO5546 (4A10)_PCR/4292 [Gene]SCO5546/7813 NC_003888 SC1C2.27, unknown, len: 261 aa; similar to hypothetical proteins e.g. E. coli TR:P76004 (EMBL:AE000216) O219 (219 aa), fasta scores; opt: 555 z-score: 764.8 E(): 0, 45.7% identity in 208 aa overlap and to e.g. HPCE_ECOLI 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (405 aa), fasta scores; opt: 389 z-score: 507.2 E(): 5.3e-21, 33.9% identity in 218 aa overlap 1 3 12 18 [Reporter]SCO2197 (5M6)_PCR/4291 [Gene]SCO2197/7811 NC_003888 SC3H12.05c, possible integral membrane protein, len: 155 aa; identical to previously sequenced TR:CAB51281 (EMBL:AL096872) Streptomyces coelicolor hypothetical 16.6 kD protein SC5F7.04c, 155 aa and similar to SW:YCKC_BACSU (EMBL:D50453) Bacillus subtilis hypothetical 17.0 kD protein in ComJ 5'region (ORF3) YckC, 151 aa; fasta scores: opt: 108 z-score: 138.4 E(): 2.5; 33.1% identity in 118 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 18 7 [Reporter]SCO1563 (16L4)_PCR/1892 [Gene]SCO1563/3459 NC_003888 SCL11.19c, possible acetyltransferase, len: 161 aa; similar to TR:O26873 (EMBL:AE000856) Methanobacterium thermoautotrophicum conserved protein Mth779, 170 aa; fasta scores: opt: 174 z-score: 231.8 E(): 1.7e-05; 28.8% identity in 125 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 3 12 17 [Reporter]SCO5817 (6I6)_PCR/4290 [Gene]SCO5817/7809 NC_003888 SC5B8.07, possible DNA hydrolase with mutT domain, l en: 294 aa; similar in part to many hypothetical proteins e .g Synechocystis sp. TR:P74291 (EMBL:D90913) (261 aa), fasta scores; opt: 241 z-score: 358.0 E(): 9.6e-13, 34.7% ident ity in 251 aa overlap. Contains PS00893 mutT domain signatu re and Pfam match to entry mutT PF00293, Bacterial mutT pro tein, score 39.86 1 3 12 15 [Reporter]SCO4748 (8A6)_PCR/4288 [Gene]SCO4748/7807 NC_003888 SC6G4.26, unknown, len: 78 aa; contains Gln-rich C-t erminus 1 3 12 14 [Reporter]SCO2652 (9M2)_PCR/4287 [Gene]SCO2652/7805 NC_003888 SC8E4A.22, hypothetical protein, len: 144 aa; similar to TR:P71036 (EMBL:Y08559) Bacillus subtilis hypothetical 14.7 kD protein YwnA, 133 aa; fasta scores: opt: 301 z-score: 389.2 E(): 2.9e-14; 38.4% identity in 138 aa overlap 1 3 12 13 [Reporter]SCO6255 (10I2)_PCR/4286 [Gene]SCO6255/7803 NC_003888 SCAH10.20, possible dehydrogenase, len: 337 aa; similar to SW:STRI_STRGR (EMBL:Y00459) Stretomyces griseus streptomycin resistance protein StrI, 348 aa; fasta scores: opt: 582 z-score: 670.0 E(): 6.1e-30; 39.5% identity in 344 aa overlap and to SW:MI2D_BACSU (EMBL;M76431) Bacillus subtilis myo-inositol dehydrogenase (EC 1.1.1.18) Idh or IolG OR e83G, 330 aa; fasta scores: opt: 534 z-score: 615.6 E(): 6.5e-27; 28.8% identity in 351 aa overlap. Contains match to Pfam entry PF01408 GFO_IDH_MocA, oxidoreductase family 1 3 12 12 [Reporter]SCO2714 (11E2)_PCR/4285 [Gene]SCO2714/7801 NC_003888 SCC61A.35, possible secreted protein, len: 395aa; contains a possible non-cleavable N-terminal signal sequence. 4 1 18 2 [Reporter]SCO7476 (20D24)_PCR/1887 [Gene]SCO7476/3449 NC_003888 SCBAC17A6.09c, possible secreted protein, len: 405aa: no significant database matches. Contains a possible N-terminal signal sequence. 1 3 23 19 [Reporter]SCO7611 (4M18)_PCR/4533 [Gene]SCO7611/8263 NC_003888 SC2H2.09, conserved hypothetical protein, len: 237 aa; similar to TR:O06738 (EMBL:Y09476) Bacillus subtilis YitC protein, 228 aa; fasta scores: opt: 320 z-score: 384.6 E(): 6.9e-14; 32.2% identity in 199 aa overlap 1 4 12 12 [Reporter]SCO2706 (11E1)_PCR/6300 [Gene]SCO2706/11481 NC_003888 SCC61A.27, possible transferase, len: 436 aa; similar to many proposed to be involved in LPS biosynthesis e.g. SW:LPSB_RHIME (EMBL:AF193023) LpsB from lipopolysaccharide biosynthesis cluster of Rhizobium meliloti. Contains Pfam match to entry PF00534 Glycos_transf_1, Glycosyl transferases group 1. Also contains a TTA encoded leucine close to the N-terminus, possible target for bldA regulation 1 3 23 18 [Reporter]SCO6703 (5I18)_PCR/4532 [Gene]SCO6703/8261 NC_003888 SC4C6.13c, pcaI, probable 3-oxoadipate CoA-transferase subunit A, len: 260 aa; highly similar to many CoA transferases e.g. SW:PCAI_PSEPU (EMBL:M88763), pcaI, Pseudomonas putida 3-oxoadipate CoA-transferase subunit A (231 aa), fasta scores; opt: 337 z-score: 379.8 E(): 8e-14, 42.6% identity in 242 aa overlap. Highly similar to SW:SCOA_MYCTU (EMBL:Z95556), scoA, Mycobacterium tuberculosis probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (248 aa) (69.9% identity in 249 aa overlap). Contains Pfam match to entry PF01144 CoA_trans, Coenzyme A transferase and PS01273 CoA transferases signature 1 1 3 23 17 [Reporter]SCO7555 (6E18)_PCR/4531 [Gene]SCO7555/8259 NC_003888 SC5F1.09, possible solute binding lipoprotein, len: 436 aa; similar to TR:Q9L151 (EMBL:AL158061) Streptomyces coelicolor probable solute binding lipoprotein SC6D11.04c, 451 aa; fasta scores: opt: 386 z-score: 436.1 E(): 9.3e-17; 27.5% identity in 459 aa overlap. Contains Pfam match to entry PF01547 SBP_bacterial_1, Bacterial extracellular solute-binding protein and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal signal peptide sequence 1 4 12 10 [Reporter]SCO3530 (12M21)_PCR/6298 [Gene]SCO3530/11477 NC_003888 SCE2.11, unknown, len: 199 aa; similar to TR:Q9L1M9 (EMBL:AL138977) Streptomyces coelicolor hypothetical protein SC7F9.38, 211 aa; fasta scores: opt: 562 Z-score: 634.5 E(): 1e-27; 44.444% identity in 189 aa overlap 1 3 23 16 [Reporter]SCO2668 (7A18)_PCR/4530 [Gene]SCO2668/8257 NC_003888 SC6D10.11, hypothetical protein, len: 452 aa; similar to TR:Y103_SYNY3 (EMBL:D64004) Synechocystis sp. hypothetical 45.8 kD protein SLL0103, 420 aa; fasta scores: opt: 265 z-score: 295.0 E(): 5.1e-09; 26.6% identity in 354 aa overlap 1 4 12 9 [Reporter]SCO0532 (13I21)_PCR/6297 [Gene]SCO0532/11475 NC_003888 SCF11.12, probable sugar transporter membrane protein, len: 302 aa; similar to TR:CAB55533 (EMBL:Y07706) Streptomyces coelicolor inner membrane protein MalF, 300 aa; fasta scores: opt: 493 z-score: 600.8 E(): 4.3e-26; 32.6% identity in 291 aa overlap. Contains a Pfam match to entry PF00528 BPD_transp, binding-protein-dependent transport systems inner membrane component and a prosite match to entry PS00402 binding-protein-dependent transport systems inner membrane component signature 1 3 23 15 [Reporter]SCO1994 (8M14)_PCR/4529 [Gene]SCO1994/8255 NC_003888 SC7H2.08, possible integral membrane protein, len: 118aa; contains possible membrane spanning hydrophobic region. 1 4 12 8 [Reporter]SCO0086 (14E21)_PCR/6296 [Gene]SCO0086/11473 NC_003888 SCJ11.15c, possible ribosylglycohydrolase (putative secreted protein), len: 312 aa; similar to SW:DRAG_RHORU (EMBL:X16187) ADP-ribosylglycohydrolase DraG from Rhodospirillum rubrum (294 aa) fasta scores; opt: 267, z-score: 299.5, E(): 2.5e-09, (31.1% identity in 283 aa overlap) 1 3 23 14 [Reporter]SCO5846 (9I14)_PCR/4528 [Gene]SCO5846/8253 NC_003888 SC9B10.13c, putative secreted protein, len: 689 aa. Contains possible N-terminal region signal peptide sequence 1 4 12 7 [Reporter]SCO0275 (15A21)_PCR/6295 [Gene]SCO0275/11471 NC_003888 SCF85.03, possible transcriptional repressor protein, len: 385 aa. Low similarity to many repressor proteins e.g. Anaerocellum thermophilum SW:XYLR_ANATH (EMBL; Z69782)xylose repressor (399 aa), fasta scores opt: 325 z-score: 375.7 E(): 1.4e-13 23.3% identity in 395 aa overlap and Streptomyces coelicolor TR:O50502 (EMBL; AL009199) probable transcriptional repressor protein SC7B7.05 (403 aa), fasta scores opt: 402 z-score: 463.7 E(): 1.8e-18 29.7% identity in 404 aa overlap. Contains a Pfam match to entry PF00480 ROK, ROK family and has a possible helix turn helix motif between aa 30..51. 1 3 23 13 [Reporter]SCO1934 (10E14)_PCR/4527 [Gene]SCO1934/8251 NC_003888 SCC22.16c, possible cytochrome oxidase assembly factor, len: 340 aa; similar to many e.g. TR:O33143 (EMBL:Z99125) cytochrome C oxidase assembly factor from Mycobacterium leprae (289 aa) fasta scores; opt: 925, z-score: 1077.3, E(): 0, (49.3% identity in 286 aa overlap) and SW:CYOE_ECOLI cytochrome O ubiquinol oxidase operon protein, CyoE from Escherichia coli (296 aa) fasta scores; opt: 520, z-score: 609.0, E(): 1.4e-26, (32.7% identity in 284 aa overlap). Contains Pfam match to entry PF01040 COX10_ctaB_cyoE, Cytochrome c oxidase assembly factor, Prosite match to PS00943 Cytochrome c oxidase assembly factor COX10/ctaB/cyoE signature and possible membrane spanning hydrophobic regions. 1 3 23 12 [Reporter]SCO4098 (11A14)_PCR/4526 [Gene]SCO4098/8249 NC_003888 SCD17.02c, probable acetyltransferase, len: 216 aa. Highly similar to many acetyltransferases involved in antibiotic resistance e.g. Enterococcus faecium SW:SATA_ENTFC (EMBL; L12033) streptogramin A acetyltransferase (EC 2.3.1.-) (209 aa), fasta scores opt: 610 z-score: 726.5 E(): 0 46.0% identity in 189 aa overlap and Enterococcus faecium TR:AAD44719 (EMBL; AF139725) SatG protein (214 aa), fasta scores opt: 665 z-score: 790.9 E(): 0 53.7% identity in 188 aa overlap. It is also highly similar to Streptomyces coelicolor SC5G9.03c (217 aa), fasta scores opt: 868 z-score: 924.4 E():0 62.0% identity in 205 aa overlap. Contains a Pfam match to entry PF00132 hexapep, Bacterial transferase hexapeptide (four repeats). 1 4 12 5 [Reporter]SCO0026 (17I17)_PCR/6293 [Gene]SCO0026/11467 NC_003888 SCJ4.07, possible secreted protein, len: 171 aa; Contains possible N-terminal signal sequence. Also contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 1 3 23 11 [Reporter]SCO4151 (12M10)_PCR/4525 [Gene]SCO4151/8247 NC_003888 SCD84.18c, possible acetyltransferase, len: 309 aa; similar to TR:O53831 (EMBL:AL022004) Mycobacterium tuberculosis hypothetical 33.6 kD protein MTV043.11, 315 aa; fasta scores: opt: 662 z-score: 773.0 E(): 0; 45.1% identity in 304 aa overlap and C-terminal region to SW:RIMI_ECOLI (EMBL:X06117) Escherichia coli ribosomal-protein-alanine acetyltransferase (EC 2.3.1.128) RimI, 148 aa; fasta scores: opt: 159 z-score: 195.2 E(): 0.0021; 31.3% identity in 112 aa overlap. Contains 2x Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 4 12 3 [Reporter]SCP1.116 (19A17)_PCR/6291 [Gene]SCP1.116/11465 NC_003888 SCP1.116, possible ECF-family sigma factor, len: 380aa; region similar to many eg. TR:Q9RIT1 (EMBL:AJ010584) ECF sigma factor from Streptomyces coelicolor (264 aa) fasta scores; opt: 485, z-score: 519.3, E(): 1.9e-21, 39.0% identity in 223 aa overlap and SW:P35165 (SIGX_BACSU) RNA polymerase sigma factor SigX from Bacillus subtilis (194 aa) fasta scores; opt: 215, z-score: 237.7, E(): 9e-06, 29.0% identity in 162 aa overlap. Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily). Contains a TTA encoded leucine at residue 31, possible target for bldA regulation. 1 3 23 10 [Reporter]SCO3114 (13I10)_PCR/4524 [Gene]SCO3114/8245 NC_003888 SCE41.23c, conserved hypothetical protein, len: 298 aa; similar to TR:O07481 (EMBL:Z97071) Streptomyces reticuli AbpS protein, 311 aa; fasta scores: opt: 482 z-score: 473.6 E(): 7.1e-19; 35.0% identity in 294 aa overlap. Contains various coiled-coil regions at approx. residues 42..60 and 207..251 1 4 12 2 [Reporter]SCO7478 (20I13)_PCR/6290 [Gene]SCO7478/11463 NC_003888 SCBAC17A6.11c, possible phosphotransferase, len: 300aa: similar to many some of which are antibiotic resistance determinants eg. TR:O05841 (EMBL:Z95120) hypothetical protein from Mycobacterium tuberculosis (474 aa) fasta scores; opt: 971, Z-score: 1047.4, 49.315% identity (50.000% ungapped) in 292 aa overlap and TR:Q53826 (EMBL:U13078) capreomycin phosphotransferase from Streptomyces capreolus (281 aa) fasta scores; opt: 162, Z-score: 183.1, 28.632% identity (30.734% ungapped) in 234 aa overlap. Contains Pfam match to entry PF01636 APH, Aminoglycoside phosphotransferase. 1 3 23 9 [Reporter]SCO0607 (14E10)_PCR/4523 [Gene]SCO0607/8243 NC_003888 SCF55.31 possible lipoprotein, len: 112 aa. Contains match to Prosite to entry correctly situated PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains also possible N-terminal region signal peptide 1 4 11 22 [Reporter]SCO1240 (1I9)_PCR/6288 [Gene]SCO1240/11461 NC_003888 2SCG1.15, possible NLP/P60 family protein (putative secreted protein), len: 157 aa; similar to C-terminal region of TR:CAB92659 (EMBL:AL356832) Streptomyces coelicolor putative NLP/P60 family secreted protein SCD63A.07c, 398 aa; fasta scores: opt: 351 z-score: 415.4 E(): 1.1e-15; 42.5% identity in 134 aa overlap. Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family. Contains possible N-terminal region signal peptide sequence 1 3 23 8 [Reporter]SCO1792 (15A10)_PCR/4522 [Gene]SCO1792/8241 NC_003888 SCI51.32c, possible 3-methyladenine DNA glycosylase, len: 213 aa; similar to several eukaryotic 3-methyladenine DNA glycosylases, but shorter at the N-terminus e.g. SW:3MG_HUMAN (EMBL:M74905) Homo sapiens (Human) DNA-3-methyladenine glycosylase (298 aa), fasta scores; opt: 410 z-score: 485.1 E(): 1.1e-19, 38.6% identity in 207 aa overlap. Also similar putative 3-methyladenine DNA glycosylases from mycobacteria e.g. SW:3MGH_MYCTU (EMBL:Z98268) Mycobacterium tuberculosis possible 3-methyladenine DNA glycosylase (203 aa) (43.0% identity in 193 aa overlap) 1 3 23 7 [Reporter]SCO0659 (16M6)_PCR/4521 [Gene]SCO0659/8239 NC_003888 SCF91.19, possible DeoR-family transcriptional regulator, len: 368 aa. Similar to many DNA-binding regulatory proteins including: Streptomyces reticuli TR:CAB46346(EMBL:AJ009798) regulator CebR (350 aa), fasta scores opt: 287 z-score: 337.9 E(): 1.9e-11 30.6% identity in 359 aa overlap and Streptomyces lividans SW:REGL_STRLI (EMBL:X98242) transcription regulatory protein RegL (345 aa), fasta scores opt: 276 z-score: 325.3 E(): 9.6e-11 28.9% identity in 353 aa overlap. Contains Pfam matches to entries PF00455 deoR, Bacterial regulatory proteins, deoR family and Pfam match to entry PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI family. In addition to a Prosite hit to PS00894 Bacterial regulatory proteins, deoR family signature with the putative helix-turn-helix motif situated between residues 23..44 (+3.43 SD). Also contains a probable coiled-coil from 21 to 48 (28 residues). 1 4 11 20 [Reporter]SCO4141 (3A9)_PCR/6286 [Gene]SCO4141/11457 NC_003888 SCD84.08c, pstC, phosphate ABC transport system permease protein, len: 336 aa; similar to TR:O86344 (EMBL:Z47983) Mycobacterium tuberculosis phosphate transport system permease protein PstC2, 324 aa; fasta scores: opt: 900 z-score: 993.3 E(): 0; 49.4% identity in 308 aa overlap and to SW:PSTC_ECOLI (EMBL:X02723) Escherichia coli phosphate transport system permease protein PstC or PhoW, 319 aa; fasta scores: opt: 667 z-score: 738.3 E(): 0; 40.5% identity in 311 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 1 3 23 4 [Reporter]SCO4875 (19A6)_PCR/4518 [Gene]SCO4875/8237 NC_003888 2SCK8.01, probable sugar transferase (fragment), len: >256 aa; similar to SW:GTRB_BPSFX (EMBL:AF056939) Bacteriophage SfX (Shigella flexneri bacteriophage X) bactoprenol glucosyl transferase (EC 2.4.1.*) GtrB, 305 aa; fasta scores: opt: 659 z-score: 787.3 E(): 0; 42.3% identity in 246 aa overlap and to middle part of TR:O86666 (EMBL:AL031182) Streptomyces coelicolor putative sugar transferase SC4A2.10c, 478 aa; fasta scores: opt: 1022 z-score: 1213.9 E(): 0; 61.5% identity in 247 aa overlap. Contains possible hydrophobic membrane spanning regions at C-terminal domain,SCK20.16, probable transferase (fragment), len: >92 aa; similar to N-terminal region of SW:GTRB_BPSF5 (EMBL:U82619) Shigella flexneri bacteriophage V bactoprenol glucosyl transferase (EC 2.4.1.-) GtrB, 307 aa; fasta scores: opt: 264 z-score: 364.3 E(): 9.3e-13; 47.3% identity in 91 aa overlap and to N-terminal region of TR:O86666 (EMBL:AL031182) Streptomyces coelicolor putative sugar transferase SC4A2.10c, 478 aa; fasta scores: opt: 341 z-score: 431.2 E(): 2.4e-18; 61.1% identity in 90 aa overlap 1 4 11 19 [Reporter]SCO6618 (4M5)_PCR/6285 [Gene]SCO6618/11455 NC_003888 SC1F2.15c, unknown, len: 226 aa 1 3 23 3 [Reporter]SCO7515 (20M2)_PCR/4517 [Gene]SCO7515/8235 NC_003888 SCBAC25F8.07, unknown, len: 189 aa 1 4 11 18 [Reporter]SCO6572 (5I5)_PCR/6284 [Gene]SCO6572/11453 NC_003888 SC3F9.07, possible glycosyl hydrolase (putative secreted protein), len: 1238 aa; similar to TR:Q9K473 (EMBL:AL359215) putative glycosyl hydrolase from Streptomyces coelicolor (962 aa) fasta scores; opt: 3614, Z-score: 3774.0, 63.899% identity (65.074% ungapped) in 831 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 22 22 [Reporter]SCO1147 (1E22)_PCR/4515 [Gene]SCO1147/8233 NC_003888 2SCG38.40, probable ABC transporter transmembrane protein (fragment), len: >35 aa,SCG8A.01, partial CDS, probable ABC transporter transmembrane subunit, len: >623aa; similar to many eg. TR:O87312 (EMBL:AF027770) FxtA iron sequestration protein from Mycobacterium smegmatis (574 aa) fasta scores; opt: 794, z-score: 866.3, E(): 0, 35.2% identity in 514 aa overlap. Contains Pfam match to entry PF00664 ABC_membrane, ABC transporter transmembrane region, Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite match to PS00211 ABC transporters family signature. Also contains possible membrane-spanning hydrophobic regions. 1 4 11 17 [Reporter]SCO2035 (6E5)_PCR/6283 [Gene]SCO2035/11451 NC_003888 SC4G6.04c, possible membrane protein, len: 275 aa; similar to TR:Q9S2S3 (EMBL:AL096884) Streptomyces coelicolor hypothetical protein SC4G6.36, 266 aa; fasta scores: opt: 519 Z-score: 588.1 E(): 4e-25; 34.470% identity in 264 aa overlap 1 3 22 21 [Reporter]SCO7343 (2A22)_PCR/4514 [Gene]SCO7343/8231 NC_003888 SC4G10.22c, hemC, porphobilinogen deaminase (EC 4.3.1.8). len: 313 aa. Highly similar to many e.g. Escherichia coli SW:HEM3_ECOLI(EMBL:X04242) porphobilinogen deaminase (EC 4.3.1.8) (313 aa), fasta scores opt: 710 z-score: 839.8 E(): 0 42.2% identity in 303 aa overlap. Contains a Pfam match to entry PF01379 Porphobil_deam, Porphobilinogen deaminase. 1 3 22 20 [Reporter]SCO6423 (3M18)_PCR/4513 [Gene]SCO6423/8229 NC_003888 SC1A6.12c, putative lipoate-protein ligase, len: 388 aa; some similarity inC-terM to LPLA_ECOLI P32099 Escherichia coli putative lipoate-protein ligase A (337 aa), fasta scores; opt: 162 z-score: 223.4E(): 2.5e-05, 27.8% identity in 252 aa overlap 1 4 11 15 [Reporter]SCO5562 (8M1)_PCR/6281 [Gene]SCO5562/11447 NC_003888 SC7A1.06, thiL, thiamine monphosphate kinase, len: 322aa; similar to many eg. TR:P95118 (EMBL:Z83018) hypothetical protein from Mycobacterium tuberculosis (333 aa) fasta scores; opt: 959, z-score: 1227.0, E(): 0, (51.1% identity in 319 aa overlap) and THIL_SALTY thiamine monphosphate kinase from Salmonella typhimurium (325 aa) fasta scores; opt: 511, z-score: 350.3, E(): 3.2e-12, (35.9% identity in 329 aa overlap). Note: thiAB (arbitrarily named) previously mapped to this region of the chromosome. 1 3 22 19 [Reporter]SCO7607 (4I18)_PCR/4512 [Gene]SCO7607/8227 NC_003888 SC2H2.05c, possible hydrolase, len: 393 aa; similar to SW:DAC_STRSQ (EMBL:M26842) Streptomyces sp. D-alanyl-D-alanine carboxypeptidase precursor (EC 3.4.16.4), 372 aa; fasta scores: opt: 313 z-score: 344.0 E(): 1.3e-11; 33.8% identity in 364 aa overlap 1 4 11 14 [Reporter]SCO2638 (9I1)_PCR/6280 [Gene]SCO2638/11445 NC_003888 SC8E4A.08c, putative membrane protein, len: 249 aa. Contains possible hydrophobic membrane spanning regions 1 3 22 18 [Reporter]SCO6826 (5E18)_PCR/4511 [Gene]SCO6826/8225 NC_003888 SC4A9.03c, unknown, len: 346 aa. Similar to a protein of undefined function encoded within the Streptomyces peucetius daunorubicin-doxorubicin polyketide synthase gene cluster: TR:Q54816 (EMBL:L35560) DpsC (353 aa), opt: 662 z-score: 779.9 E(): 0 36.0% identity in 344 aa overlap. Also similar to Streptomyces sp. TR:Q55225 (EMBL:L34880) OrfC, a beta-keto acyl synthase III homologue (352 aa), fasta scores opt: 599 z-score: 706.2 E(): 6.3e-32 33.4% identity in 341 aa overlap 1 4 11 13 [Reporter]SCO6478 (10E1)_PCR/6279 [Gene]SCO6478/11443 NC_003888 SC9C7.14c, conserved hypothetical protein, len: 132aa; similar to many hypotheticals eg. TR:P75896 (EMBL:AE000202) from Escherichia coli (128 aa) fasta scores; opt: 249, z-score: 350.3, E(): 3.2e-12, (39.2% identity in 125 aa overlap). Contains Pfam match to entry PF01042 DUF10, Domain of unknown function. 1 3 22 17 [Reporter]SCO7551 (6A18)_PCR/4510 [Gene]SCO7551/8223 NC_003888 SC5F1.05c, putative secreted protein, len: 67 aa. Contains possible N-terminal region signal peptide sequence 1 4 11 12 [Reporter]SCO2702 (11A1)_PCR/6278 [Gene]SCO2702/11441 NC_003888 SCC61A.23c, possible secreted protein, len: 171aa; contains a possible cleavable N-terminal signal sequence. 1 3 22 16 [Reporter]SCO1414 (7M14)_PCR/4509 [Gene]SCO1414/8221 NC_003888 SC6D7.25. possible ankyrin-like protein, len: 134 aa. Similar to many Prokaryotic and Eukaryotic proteins containing ankyrin repeat domains e.g. Pseudomonas aeruginosa TR:Q51402(EMBL:U59457) AnkB (ankyrin) (180 aa), fasta scores opt: 263 z-score: 329.0 E(): 6e-11 40.7% identity in 118 aa overlap and Xiphophorus helleri TR:AAD21313(EMBL:AF132500) P13CDKN2X (124 aa), fasta scores opt: 192 z-score: 246.7 E(): 2.3e-06 34.7% identity in 121 aa overlap. Also similar to Streptomyces coelicolor (EMBL:AL121855) hypothetical protein SCF62.06 (131 aa), fasta scores opt:387 z-score: 407.2 E(): 2.5e-17 53.8% identity in 119 aa overlap. Contains 2xPfam match to entry PF00023 ank, Ank repeat. 1 3 22 15 [Reporter]SCO1990 (8I14)_PCR/4508 [Gene]SCO1990/8219 NC_003888 SC7H2.04c, unknown, len: 237aa; similar to SW:Y08N_MYCTU hypothetical protein from Mycobacterium tuberculosis (350 aa) fasta scores; opt: 870, z-score: 990.4, E(): 0, (57.1% identity in 231 aa overlap). 1 4 11 10 [Reporter]SCO3526 (12I21)_PCR/6276 [Gene]SCO3526/11437 NC_003888 SCE2.07, hypothetical protein, len: 427 aa; C-terminal region similar to TR:Q9L1W2 (EMBL:AL138667) Streptomyces coelicolor hypothetical protein (fragment) SC3D9.17c, 235 aa; fasta scores: opt: 1120 Z-score: 1250.9 E(): 4.8e-62; 71.795% identity in 234 aa overlap 1 3 22 14 [Reporter]SCO7527 (9E14)_PCR/4507 [Gene]SCO7527/8217 NC_003888 SC8G12.03c, possible DeoR-family transcriptional regulator, len: 343 aa. Similar to several e.g. Streptomyces coelicolor TR:CAB81864 (EMBL:AL161691) putative DeoR-family transcriptional regulator, SCD40A.19C (339 aa), fasta scores opt: 600 z-score: 674.6 E(): 4.1e-30 41.1% identity in 316 aa overlap. Contains a Prosite hit to PS00894 Bacterial regulatory proteins, deoR family signature with a putative helix-turn-helix motif situated between residues 31..52 (+2.89 SD). 1 4 11 9 [Reporter]SCO5023 (13E21)_PCR/6275 [Gene]SCO5023/11435 NC_003888 SCK15.25, possible secreted protein, len: 336 aa; similar to TR:CAB77312 (EMBL:AL160312) Streptomyces coelicolor putative secreted protein SCD35.28c, 361 aa; fasta scores: opt: 890 z-score: 972.2 E(): 0; 47.3% identity in 357 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 22 13 [Reporter]SCO1930 (10A14)_PCR/4506 [Gene]SCO1930/8215 NC_003888 SCC22.12, possible integral membrane transport protein, len: 356aa; function predicted as it contains possible membrane spanning hydrophobic regions and is located alongside a possible ABC transport ATP-binding protein (SCC22.10). Similar to TR:O53148 (EMBL:AL021184) hypothetical protein from Mycobacterium tuberculosis (310 aa) fasta scores; opt: 527, z-score: 648.1, E(): 9.2e-29, (35.0% identity in 300 aa overlap). 1 4 11 8 [Reporter]SCO0169 (14A21)_PCR/6274 [Gene]SCO0169/11433 NC_003888 SCJ1.18, conserved hypothetical protein, len: 217 aa. Duplication of DNA region complement(17965..18576). Similar to many hypothetical proteins including: Thermotoga maritima TR:AAD36216 (EMBL; AE001771) conserved hypothetical protein (215 aa), fasta scores opt: 239 z-score: 287.3 E(): 1.2e-08 29.1% identity in 175 aa overlap and S. coelicolor SCJ1.19c (223 aa), fasta scores opt: 711 z-score: 702.0 E(): 7.1e-34 56.7% identity in 215 aa overlap.Contains 2xPfam match to entry PF00571 CBS, CBS domains. 1 3 22 12 [Reporter]SCO4332 (11M10)_PCR/4505 [Gene]SCO4332/8213 NC_003888 SCD12A.15c, possible integral membrane ATPase, len: 802 aa; similar to TR:Q9Z4W5 (EMBL:AL035654) Streptomyces coelicolor putative integral membrane ATPase SCE8.09, 796 aa; fasta scores: opt: 3320 z-score: 3591.6 E(): 0; 67.0% identity in 796 aa overlap and to TR:O93862 (EMBL:AF043332) Aspergillus nidulans plasma membrane H(+)ATPase PmaA, 990 aa; fasta scores: opt: 504 z-score: 543.3 E(): 8.4e-23; 25.4% identity in 808 aa overlap. Contains 2x Pfam match to entry PF00122 E1-E2_ATPase, E1-E2 ATPase and match to Prosite entry PS00154 E1-E2 ATPases phosphorylation site. Contains also possible hydrophobic membrane spanning regions 1 4 11 7 [Reporter]SCO0183 (15M17)_PCR/6273 [Gene]SCO0183/11431 NC_003888 SCJ1.32, unknown,len: 415 aa. Likely to have arisen as a result of a duplication of SCF43A.31/32, followed by a deletion event generating a possible CDS with the 5' coding sequence of SCF43A.31 and the 3' coding sequence of SCF43A.32. Similarity scores are as follows: to Streptomyces coelicolor TR:CAB48918 (EMBL; AL096837) putative oxidoreductase SCF43A.31 (520 aa), fasta scores opt: 1191 z-score: 1365.3 E(): 0 64.2% identity in 307 aa overlap and to Streptomyces coelicolor TR:CAB48919(EMBL; AL096837) putative deoxyribodipyrimidine photolyase SCF43A.32 (458 aa), opt: 909 z-score: 1043.2 E(): 0 45.7% identity in 418 aa overlap. Contains a PS00394 DNA photolyases class 1 signature 1 and a PS00691 DNA photolyases class 1 signature 2. 1 3 22 11 [Reporter]SCO4147 (12I10)_PCR/4504 [Gene]SCO4147/8211 NC_003888 SCD84.14, possible integral membrane protein, len: 287 aa. Contains possible hydrophobic membrane spanning regions 1 3 22 10 [Reporter]SCO3250 (13E10)_PCR/4503 [Gene]SCO3250/8209 NC_003888 SCE29.19c, probable integrase, len: 384aa; similar to many eg. SW:INTR_STRAM integrase from the integrated conjugative pSAM plasmid of Streptomyces ambofaciens (388 aa) fasta scores; opt: 445, z-score: 549.1, E(): 2.8e-23, (26.8% identity in 392 aa overlap). Contains Pfam match to entry PF00589 Phage_integrase, 'Phage' integrase family, score 88.00, E-value 1.9e-22. 1 4 11 5 [Reporter]SCO0152 (17E17)_PCR/6271 [Gene]SCO0152/11427 NC_003888 SCJ1.01, possible transmembrane transport protein, partial CDS, len: >284 aa; similar to S. coelicolor putative transmembrane transport protein SC5B8.17 TR:O70003 (EMBL: AL022374) (530 aa), fasta scores; opt: 513 z-score: 549.9 E(): 2.9e-23 38.1% identity in 270 aa overlap, Rhodobacter capsulatus transmembrane protein TR:O68042(EMBL: AF010496)(578 aa), fasta scores opt: 243 z-score: 263.6 E(): 2.5e-07 26.3% identity in 281 aa overlap and Pseudomonas putida TR:O50471 (EMBL; Z71176) phenylacetic acid permease (520 aa), fasta scores opt: 233 z-score: 253.6 E(): 9.1e-07 31.1% identity in 293 aa overlap. Contains probable membrane spanning hydrophobic domains.,SCJ33.16, possible transmembrane transport protein, partial CDS, len: >288 aa. Similar to several including: Pseudomonas putida TR:O50471 (EMBL; Z71176) phenylacetic acid permease (520 aa), fasta scores opt: 337 z-score: 380.9 E(): 7.5e-14 29.4% identity in 269 aa overlap and Streptomyces coelicolor TR:O70003 (EMBL; AL022374) putative transmembrane transport SC5B8.17 (530 aa), fasta scores opt: 462 z-score: 520.1 E(): 1.3e-21 31.9% identity in 279 aa overlap. Overlaps with and extends into CDS SCJ1.01. Contains possible membrane spanning hydrophobic domains. 1 3 22 9 [Reporter]SCO0747 (14A10)_PCR/4502 [Gene]SCO0747/8207 NC_003888 SCF81.06c, hypothetical protein, len: 166 aa; similar to various hypothetical proteins, e.g. TR:AAD36153 (EMBL:AE001768) Thermotoga maritima conserved hypothetical protein TM1076, 157 aa; fasta scores: opt: 293 z-score: 385.9 E(): 4e-14; 39.1% identity in 133 aa overlap and to TR:Q9CCZ0 (EMBL:AL583926) Mycobacterium leprae conserved hypothetical protein ML 2654, 165 aa; fasta scores: opt: 642 Z-score: 785.7 E(): 4e-36; 60.248% identity in 161 aa overlap 1 4 11 3 [Reporter]SCP1.121 (19M13)_PCR/6269 [Gene]SCP1.121/11425 NC_003888 SCP1.121, unknown, len: 178aa; Contains a TTA encoded leucine at residue 76, possible target for bldA regulation. 1 3 22 8 [Reporter]SCO3917 (15M6)_PCR/4501 [Gene]SCO3917/8205 NC_003888 SCH24.39c, hypothetical protein, len: 288 aa; unknown function, similar to TR:O06743 (EMBL:Y09476), yitH, Bacillus subtilis hypothetical protein (282 aa), fasta scores; opt: 288 z-score: 349.1 E(): 3.9e-12, 26.8% identity in 231 aa overlap and TR:P73419 (EMBL:D90906) Synechocystis sp. hypothetical protein (285 aa) (21.9% identity in 265 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family, score 17.40, E-value 0.02 1 4 11 2 [Reporter]SCO7701 (20E13)_PCR/6268 [Gene]SCO7701/11423 NC_003888 SCBAC12C8.02, possible methyltransferase, len: 396aa: similar to many eg. TR:Q9F1V7 (EMBL:AB040071) methyltransferase from Streptomyces griseus (316 aa) fasta scores; opt: 1550, Z-score: 1809.5, 82.246% identity (82.545% ungapped) in 276 aa overlap. Previously sequenced as TR:Q9F1Y5 (EMBL:AB035202) ORF4. 1 3 22 7 [Reporter]SCO3208 (16I6)_PCR/4500 [Gene]SCO3208/8203 NC_003888 SCE8.01, possible secreted protein, len: 432 aa; similar to TR:CAC44281 (EMBL:AL596030) Streptomyces coelicolor hypothetical protein SCBAC17F8.09, 436 aa; fasta scores: opt: 555 Z-score: 578.7 E(): 1.3e-24; 33.991% identity in 456 aa overlap. Contains a possible N-terminal signal sequence 1 4 10 22 [Reporter]SCO5266 (1E9)_PCR/6266 [Gene]SCO5266/11421 NC_003888 2SC7G11.28, putative membrane protei, len: 277 aa; similar to N-terminal region of TR:CAB76994 (EMBL:AL159178) Streptomyces coelicolor putative integral membrane protein SCH22.25, 456 aa; fasta scores: opt: 141 z-score: 157.1 E(): 0.28; 29.3% identity in 263 aa overlap. Contains matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Contains possible hydrophobic membrane spanning region 1 3 22 6 [Reporter]SCO3353 (17E6)_PCR/4499 [Gene]SCO3353/8201 NC_003888 SCE94.04c, unknown, len: 126aa; 1 4 10 15 [Reporter]SCO5558 (8I1)_PCR/6259 [Gene]SCO5558/11407 NC_003888 SC7A1.02, possible acyltransferase, len: 264aa; similar to several proposed acyltransferases eg. TR:O07809 (EMBL:Z97188) acyltransferase from Mycobacterium tuberculosis (251 aa) fasta scores; opt: 266, z-score: 384.5, E(): 4e-14, (29.7% identity in 229 aa overlap). 1 4 10 14 [Reporter]SCO6599 (9E1)_PCR/6258 [Gene]SCO6599/11405 NC_003888 SC8A6.20c, probable transcriptional regulator, len: 217 aa; some similarity to members of the tetR family e.g. TER4_ECOLI tetracycline repressor protein class D (217 aa), fasta scores; opt: 158 z-score: 233.4 E(): 9.2e-06, 35.2% identity in 88 aa overlap. Contains probable helix-turn-helix motif at aa 29-50 (Score 1279, +3.54 SD) and Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family, score 31.90, E-value 4.2e-07 1 4 10 13 [Reporter]SCO6474 (10A1)_PCR/6257 [Gene]SCO6474/11403 NC_003888 SC9C7.10c, possible transcriptional regulator, len: 272 aa; similar to many hypotheticals and SW:TCMR_STRGA TcmR, tetracenomycin C transcriptional repressor from Streptomyces glaucescens (226 aa) fasta scores; opt: 221, z-score: 260.3, E(): 3.3e-07, (31.7% identity in 221 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family. Also contains a probable helix-turn-helix from residue 53 to 74 (+3.47 SD). 1 4 10 12 [Reporter]SCO2754 (10M21)_PCR/6256 [Gene]SCO2754/11401 NC_003888 SCC57A.25, unknown, len: 268 aa. Weakly similar to Streptomyces coelicolor TR:Q9Z596(EMBL:AL035478) putative oxidoreductase (279 aa), fasta scores opt: 185 z-score: 212.6 E(): 0.00019 29.6% identity in 247 aa overlap. 1 3 12 11 [Reporter]SCO4453 (12A2)_PCR/4284 [Gene]SCO4453/7799 NC_003888 SCD6.31c, hypothetical protein, len: 135 aa; similar to TR:O86797 (EMBL:AL031317) Streptomyces coelicolor hypothetical 13.2 kD protein, 118 aa; fasta scores: opt: 158 z-score: 203.7 E(): 0.00069; 34.0% identity in 103 aa overlap 1 3 12 10 [Reporter]SCO2828 (12M22)_PCR/4283 [Gene]SCO2828/7797 NC_003888 SCE20.02, probable amino acid ABC transporter protein, solute-binding component, len: 309 aa. Similar to several including: Salmonella typhimurium SW:ARGT_SALTY(EMBL:V01368) lysine-arginine-ornithine-binding periplasmic protein precursor (260 aa), fasta scores opt: 251 z-score: 294.5 E(): 5.1e-09 25.8% identity in 279 aa overlap and Rhizobium sp. (strain NGR234) SW:Y4TE_RHISN(EMBL:AE000098) probable amino-acid ABC transporter periplasmic binding protein Y4TE (300 aa), fasta scores opt: 599 z-score: 691.7 E(): 3.8e-31 35.4% identity in 297 aa overlap. Contains a Pfam match to entry PF00497 SBP_bac_3, Bacterial extracellular solute-binding proteins, family 3. Also contains an N-terminal possible membrane spanning hydrophobic domain. 1 3 12 9 [Reporter]SCO1775 (13I22)_PCR/4282 [Gene]SCO1775/7795 NC_003888 SCI51.15c, conserved hypothetical protein, len: 211 aa; unknown function, similar to many bacterial hypothetical proteins e.g. TR:O33199 (EMBL:Z98268) Mycobacterium tuberculosis hypothetical protein (207 aa), fasta scores; opt: 508 z-score: 616.4 E(): 5.5e-27, 43.1% identity in 197 aa overlap. Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein 1 3 12 8 [Reporter]SCO5035 (14E22)_PCR/4281 [Gene]SCO5035/7793 NC_003888 SCK7.08c, possible ABC transporter ATP-binding protein, len: 229 aa; similar to TR:CAB81857 (EMBL:AL161691) Streptomyces coelicolor putative ABC transporter ATP-binding protein SCD40A.12c, 246 aa; fasta scores: opt: 597 z-score: 668.0 E(): 9.7e-30; 47.6% identity in 206 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 12 7 [Reporter]SCO3861 (15A22)_PCR/4280 [Gene]SCO3861/7791 NC_003888 SCH69.31c, putative membrane protein, len: 441 aa. Contains possible hydrophobic membrane spanning regions 1 3 12 6 [Reporter]SCO3754 (16M18)_PCR/4279 [Gene]SCO3754/7789 NC_003888 SCH63.01c, possible ABC transport system integral membrane protein (fragment), len: >319 aa; similar to TR:CAB77000 (EMBL:AL159178) Streptomyces coelicolor putative integral membrane protein SCH22A.31, 850 aa; fasta scores: opt: 510 z-score: 493.4 E(): 5.8e-20; 38.1% identity in 315 aa overlap. Contains possible hydrophobic membrane spanning regions,SCH22A.32c, possible transmembrane transport protein (fragment), len: >569 aa; similar to TR:Q9XAA6 (EMBL:AL096837) Streptomyces coelicolor putative transmembrane transport protein SCF43A.09 463 a; fasta scores: opt: 255 z-score: 268.0 E(): 1.6e-07; 30.4% identity in 415 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 12 5 [Reporter]SCO0278 (17I18)_PCR/4278 [Gene]SCO0278/7787 NC_003888 SCF85.06, MutT domain containing protein, len: 204. Similar to the MutT domains of other proteins e.g. Bacillus subtilis SW:YZGD_BACSU (EMBL:U17168) hypothetical 45.4 KD protein in thiaminase (413 aa), fasta scores opt: 138 z-score: 176.6 E(): 0.017 36.1% identity in 97 aa overlap. Contains a Pfam match to entry PF00293 mutT, Bacterial MutT protein. 1 3 12 4 [Reporter]SCO0471 (18E18)_PCR/4277 [Gene]SCO0471/7785 NC_003888 SCF76.11, possible araC family transcriptional regulator, len: 326 aa. Weakly similar to the DNA-binding domains of other transcriptional regulators e.g. Escherichia coli SW:ARAC_ECOLI (EMBL; V00256) arabinose operon regulatory protein (292 aa), fasta scores opt: 130 z-score: 151.4 E(): 0.45 28.2% identity in 163 aa overlap. Contains a Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. The putative helix-turn-helix motif is located between residues 197..218 (+2.74 SD). Also contains a possible membrane spanning hydrophobic domain. 1 3 12 3 [Reporter]SCO4877 (19A18)_PCR/4276 [Gene]SCO4877/7783 NC_003888 2SCK8.03c, unknown, len: 444 aa 1 3 12 2 [Reporter]SCO5973 (20I14)_PCR/4275 [Gene]SCO5973/7781 NC_003888 SCBAC16H6.08, possible phosphatase, len: 842 aa: similar to TR:O69213 (EMBL:AJ224354) protein serine-threonine phosphatase from Anabaena sp. (strain PCC 7120) fasta scores; opt: 2173, Z-score: 2321.6, 52.962% identity (54.808% ungapped) in 861 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 11 22 [Reporter]SCO1252 (1I10)_PCR/4273 [Gene]SCO1252/7779 NC_003888 2SCG1.27, possible integral membrane protein, len: 337 aa; similar to TR:Q9RKZ3 (EMBL:AL133213) Streptomyces coelicolor putative integral membrane protein SC6D7.04, 347 aa; fasta scores: opt: 1101 z-score: 1230.5 E(): 0; 50.7% identity in 337 aa overlap. Contains Pfam matches to entries PF01595 DUF21, Domain of unknown function DUF21 and 2x PF00571 CBS, CBS domain. Also contains possible N-terminal region signal peptide sequence (seems to be cleavable) and possible hydrophobic membrane spanning region 4 3 23 15 [Reporter]SCO1997 (8P14)_PCR/6040 [Gene]SCO1997/10997 NC_003888 SC7H2.11c, unknown, len: 312 aa; similar to TR:O07213 (EMBL:Z96072) hypothetical protein from Mycobacterium tuberculosis (324 aa) fasta scores; opt: 770, z-score: 866.1, E(): 0, (40.9% identity in 286 aa overlap) 1 3 11 21 [Reporter]SCO1648 (2E10)_PCR/4272 [Gene]SCO1648/7777 NC_003888 SCI41.31c, arc AAA ATPase, len: 588 aa. Identical to TR:O87594 (EMBL:AF086832) Streptomyces coelicolor ARC, AAA ATPase forming ring-shaped complexes and similar to TR:O50202 (EMBL:AF088800) Rhodococcus erythropolis ARC protein, 591 aa; fasta scores: opt: 2036 z-score: 2155.8 E():0; 67.1% identity in 587 aa overlap. Pfam match to entry PF00004 AAA, ATPases associated with various cellular activities (AAA). Contains probable coiled-coil from 47 to 93 (47 residues, max score: 1.592, probability 0.99) 4 3 23 14 [Reporter]SCO5851 (9L14)_PCR/6039 [Gene]SCO5851/10995 NC_003888 SC9B10.18, possible integral membrane protein, len: 312 aa. Contains possible hydrophobic membrane spanning regions 1 3 11 20 [Reporter]SCO4086 (3A10)_PCR/4271 [Gene]SCO4086/7775 NC_003888 SCD25.22, purF, amidophosphoribosyltransferase, len: 530 aa. Identical to Streptomyces lividans TR:Q9ZB04 (EMBL; U64826) glutamine phosphoribosylpyrophosphate amidotransferase (EC 2.4.2.14) (530 aa), fasta scores opt: 3480 z-score: 3887.1 E(): 0 99.8% identity in 530 aa overlap and highly similar to Synechococcus sp. (strain PCC 7942) SW:PUR1_SYNP7 (EMBL; U33211) amidophosphoribosyltransferase precursor (EC 2.4.2.14) (glutamine phosphoribosylpyrophosphate amidotransferase) (ATase) (GPATase) (493 aa), fasta scores opt: 1630 z-score: 1822.2 E(): 0 52.5% identity in 495 aa overlap. Contains 2x Pfam match to entry PF00310 GATase_2, Glutamine amidotransferases class-II and a Prosite hit to PS00103 Purine/pyrimidine phosphoribosyl transferases signature. 4 3 23 13 [Reporter]SCO1938 (10H14)_PCR/6038 [Gene]SCO1938/10993 NC_003888 SCC22.20, hypothetical protein, len: 351 aa; similar to TR:O88016 (EMBL:AL031107) hypothetical protein from Streptomyces coelicolor (311 aa) fasta scores; opt: 1363, z-score: 1605.9, E(): 0, (71.3% identity in 300 aa overlap) and TR:O06813 (EMBL:Z95844) hypothetical protein from Mycobacterium tuberculosis (303 aa) fasta scores; opt: 849, z-score: 1002.6, E(): 0, (46.3% identity in 309 aa overlap). 1 3 11 19 [Reporter]SCO6626 (4M6)_PCR/4270 [Gene]SCO6626/7773 NC_003888 SC1F2.23, unknown, len: 1557 aa; some simlarity to several protein kinases e.g. ABL_MLVAB tyrosine-protein kinase transforming protein from Abelson murine leukemia virus (746 aa), fasta scores; opt: 84 z-score: 234.0 E(): 8.6e-06, 24.7% identity in 364 aa overlap. Contains PS00107 Protein kinases ATP-binding region signature, Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain, score 18.10, E-value 0.00062 and possible helix-turn-helix motif at aa 1027-1048 (Score 1753, +5.16 SD) 4 3 23 12 [Reporter]SCO4101 (11D14)_PCR/6037 [Gene]SCO4101/10991 NC_003888 SCD17.05, possible secreted protein, len: 130 aa. Contains a possible N-terminal signal sequence. 1 3 11 18 [Reporter]SCO6580 (5I6)_PCR/4269 [Gene]SCO6580/7771 NC_003888 SC8A6.01, unknown, partial CDS, len: >215 aa; overlaps and extends SC3F9.15,SC3F9.15, unknown, partial CDS len: >224 aa 1 3 11 17 [Reporter]SCO2044 (6E6)_PCR/4268 [Gene]SCO2044/7769 NC_003888 SC4G6.13c, possible phosphoribosyl-AMP cyclohydrolase, len: 128aa; similar to but shorter than many eg. SW:HIS2_ECOLI phosphoribosyl-AMP cyclohydrolase from Escherichia coli (203 aa) fasta scores; opt: 343, z-score: 429.1, E(): 1.4e-16, (52.7% identity in 93 aa overlap). Also similar to SW:HIS3_MYCTU phosphoribosyl-AMP cyclohydrolase from Mycobacterium tuberculosis (115 aa) fasta scores; opt: 471, z-score: 588.3, E(): 1.9e-25, (64.2% identity in 109 aa overlap). 4 3 23 10 [Reporter]SCO3117 (13L10)_PCR/6035 [Gene]SCO3117/10987 NC_003888 SCE41.26, unknown, len: 176 aa 1 3 11 16 [Reporter]SCO7277 (7A6)_PCR/4267 [Gene]SCO7277/7767 NC_003888 SC5H1.15c, possible regulator protein, len: 151 aa; similar to Streptomyces fradiae beta-lactamase regulator protein (EMBL:AB024056) (139 aa), fasta scores; opt: 177 z-score: 241.8 E(): 3.8e-06, 35.2% identity in 122 aa overlap and TR:O54111 (EMBL:AL021529), SC10A5.20, S.coelicolor hypothetical protein (171 aa) (35.4% identity in 99 aa overlap) 4 3 23 9 [Reporter]SCO1633 (14H10)_PCR/6034 [Gene]SCO1633/10985 NC_003888 SCI41.16c, probable secreted protein, len: 95 aa. Similar to various hypothetical proteins, e.g. SW:YY34_MYCLE (EMBL:U00017) Mycobacterium leprae hypothetical 9.8 kd protein U2126B, 88 aa; fasta scores: opt: 221 z-score: 258.0 E(): 5.3e-07; 45.3% identity in 86 aa overlap 1 3 11 15 [Reporter]SCO5570 (8M2)_PCR/4266 [Gene]SCO5570/7765 NC_003888 SC7A1.14, unknown, len: 217 aa; similar to Y06F_MYCTU hypothetical protein from Mycobacterium tuberculosis (207 aa) fasta scores; opt: 244, z-score: 699.9, E(): 1.1e-31, (45.5% identity in 189 aa overlap). 4 3 23 7 [Reporter]SCO1201 (16P6)_PCR/6032 [Gene]SCO1201/10983 NC_003888 2SCG58.01, possible reductase (fragment), len: >43 aa; C-terminal part of TR:Q9RJW9 (EMBL:AL133210) Streptomyces coelicolor putative reductase (fragment) SCF37.32, 311 aa,SCG11A.32, possible reductase, len: >310 aa; similar to TR:Q9Z9W3 (EMBL:AB011836) Bacillus sp. (strain C-125) alkyl hydroperoxide reductase large subunit (EC 1.6.99.3) AhpF, 305 aa; fasta scores: opt: 668 z-score: 747.2 E(): 0; 37.3% identity in 295 aa overlap and to SW:AHPF_PSEPU (EMBL:AB010689) Pseudomonas putida alkyl hydroperoxide reductase subunit F (EC 1.6.4.-) AhpF, 520 aa; fasta scores: opt: 338 z-score: 378.9 E(): 9.9e-14; 28.6% identity in 308 aa overlap 1 3 11 14 [Reporter]SCO2648 (9I2)_PCR/4265 [Gene]SCO2648/7763 NC_003888 SC8E4A.18c, possible membrane protein, len: 95 aa. Contains possible hydrophobic membrane spanning regions 4 3 23 6 [Reporter]SCO0801 (17L6)_PCR/6031 [Gene]SCO0801/10981 NC_003888 SCF43.12, possible cytochrome P450, len: 527 aa. Highly similar to the N-terminal P450 domain of Bacillus megaterium SW:CPXB_BACME (EMBL:J04832) cytochrome P450(BM-3) (1048 aa), fasta scores opt: 789 z-score: 839.8 E():0 41.9% identity in 497 aa overlap. Also weakly similar to Eukaryotic cytochrome P450 proteins e.g. Homo sapiens (Human) SW:CPF3_HUMAN(EMBL:D12620) cytochrome P450 4F3 (leukotriene-B4 20-monooxygenase) (EC 1.14.13.30) (520 aa), fasta scores opt: 553 z-score: 592.7 E(): 1.2e-25 29.2% identity in 390 aa overlap. Contains Pfam match to entry PF00067 p450, Cytochrome P450 and a Prosite hit to PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 1 3 11 13 [Reporter]SCO6486 (10E2)_PCR/4264 [Gene]SCO6486/7761 NC_003888 SC9C7.22, possible transport associated protein, len: 277aa; similar to SW:DPPA_BACSU dppA, dipeptide transport protein (274 aa) fasta scores; opt: 448, z-score: 604.3, E(): 2.3e-26, (32.3% identity in 269 aa overlap). 1 3 11 12 [Reporter]SCO2710 (11A2)_PCR/4263 [Gene]SCO2710/7759 NC_003888 SCC61A.31, possible polysaccharide deacetylase, len: 229 aa; weakly similar to many e.g. TR:Q9XDJ5 (EMBL:AF048749) putative deacetylase from Bacteroides fragilis (259 aa) fasta scores; opt: 135, z-score: 169.7, E(): 0.054, 22.6% identity in 137 aa overlap. Contains Pfam match to entry PF01522 Polysac_deacet, Polysaccharide deacetylase. 4 3 23 4 [Reporter]SCP1.239c (19D6)_PCR/6029 [Gene]SCP1.239c/10977 NC_003888 SCP1.239c, mmyG, possible oxidoreductase, len: 393aa; previously sequenced and annotated as TR:Q9JN86 (EMBL:AJ276673). Similar to many eg. TR:Q9RK99 (EMBL:AL117322) putative oxidoreductase from Streptomyces coelicolor (371 aa) fasta scores; opt: 675, z-score: 754.9, E(): 0, 36.4% identity in 357 aa overlap. Contains Pfam match to entry PF01408 GFO_IDH_MocA, Oxidoreductase family. 1 3 11 11 [Reporter]SCO4263 (11M22)_PCR/4262 [Gene]SCO4263/7757 NC_003888 SCD49.04, possible transcriptional regulatory protein, len: 1251 aa; N-terminal domain similar to TR:Q9RD3 (EMBL:AL133422) Streptomyces coelicolor putative transcriptional regulatory protein SCM1.10, 888 aa; blastp scores: Score = 154 (54.2 bits), Expect = 5.7e-15, Sum P(2) = 5.7e-15 Identities = 120/425 (28%), Positives = 176/425 (41%) and C-terminal domain similar to TR:Q9ZGI0 (EMBL:AF079139) Streptomyces venezuelae putative transcriptional activator PkiD, 928 aa; fasta scores: opt: 602 z-score: 498.1 E(): 2.9e-20; 29.0% identity in 815 aa overlap. Contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Contains a helix-turn-helix motif at residues 1196..1217 (+3.89 SD), also a TTA leucine codon, possible target for bldA regulation and 4x degenerate repeat unit: (A/S)(A/S)AP(G/D)AAYXQAP(P/S)SDG(A/V)Y 4 3 23 3 [Reporter]SCO4889 (20P2)_PCR/6028 [Gene]SCO4889/10975 NC_003888 2SCK8.15, probable cytidine deaminase, len: 130 aa; similar to SW:CDD_HUMAN (EMBL:L27943) Homo sapiens cytidine deaminase (EC 3.5.4.5) Cda or Cdd, 146 aa; fasta scores: opt: 297 z-score: 380.0 E(): 1.3e-13; 36.2% identity in 130 aa overlap and to TR:53367 (EMBL:AL021841) Mycobacterium tuberculosis cytidine deaminase Cdd or MTV016.15c, 133 aa; fasta scores: opt: 518 z-score: 652.9 E(): 8.4e-29; 61.1% identity in 126 aa overlap. Contains Pfam match to entry PF00383 dCMP_cyt_deam, Cytidine and deoxycytidylate deaminase zinc-binding region and match to Prosite entry PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature 1 3 11 10 [Reporter]SCO3534 (12I22)_PCR/4261 [Gene]SCO3534/7755 NC_003888 SCE2.15, possible large ATP-binding protein, len: 840aa; contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) and a probable coiled-coil region around 600-650aa. 4 3 22 22 [Reporter]SCO1087 (1H22)_PCR/6026 [Gene]SCO1087/10973 NC_003888 2SCG4.03c, probable aldolase, len: 356 aa; similar to TR:O50584 (EMBL:AB001577) Pseudomonas sp. low specificity L-threonine aldolase, 346 aa; fasta scores: opt: 832 z-score: 977.3 E(): 0; 41.3% identity in 339 aa overlap and to SW:LTAA_AERJA (EMBL:D87890) Aeromonas jandaei L-allo-threonine aldolase (EC 4.1.2.-) LtaA, 338 aa; fasta scores: opt: 270 z-score: 321.1 E(): 2.1e-10; 26.9% identity in 324 aa overlap 1 3 11 9 [Reporter]SCO1636 (13E22)_PCR/4260 [Gene]SCO1636/7753 NC_003888 SCI41.19c, unknown, len: 360 aa. Similar to various hypothetical proteins, e.g. TR:P72265 (EMBL:Z82004) Rhodococcus erythropolis ORF11, 326 aa; fasta scores: opt: 694 z-score: 768.9 E(): 0; 39.8% identity in 322 aa overlap 4 3 22 21 [Reporter]SCO4469 (2D22)_PCR/6025 [Gene]SCO4469/10971 NC_003888 SCD65.12, hemL, glutamate-1-semialdehyde 2,1-aminomutase, len: 438 aa; highly similar to SW:GSA_ECOLI (EMBL:X53696) Escherichia coli glutamate-1-semialdehyde 2,1-aminomutase (EC 5.4.3.8) HemL, 426 aa; fasta scores: opt: 1425 z-score: 1497.5 E(): 0; 52.0% identity in 425 aa overlap. Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate and match to Prosite entry PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site 1 3 11 8 [Reporter]SCO0552 (14A22)_PCR/4259 [Gene]SCO0552/7751 NC_003888 SCF11.32c, possible response regulator, len: 238 aa; similar to TR:CAB54568 (EMBL:AJ006392) Streptococcus pneumoniae response regulator Rro2, 234 aa; fasta scores: opt: 583 z-score: 693.9 E(): 2.8e-31; 43.2% identity in 227 aa overlap. Contains Pfam matches to entries PF00486 trans_reg_C, transcriptional regulatory protein and PF00072 response_reg, response regulator receiver domain 4 1 16 18 [Reporter]SCO6373 (5P12)_PCR/1859 [Gene]SCO6373/3399 NC_003888 SC4A2.09, probable integral membrane protein, len: 465 aa. Contains possible hydrophobic membrane spanning regions 1 3 11 7 [Reporter]SCO0222 (15M18)_PCR/4258 [Gene]SCO0222/7749 NC_003888 SCJ9A.01, possible acetyltransferase, len: 177 aa; similar to many e.g. TR:Q53796 (EMBL:L26955) bleomycin acetyltransferase from Streptomyces verticillus (301 aa) fasta scores; opt: 150, z-score: 190.2, E(): 0.0031, (30.8% identity in 169 aa overlap). Also similar to TR:CAB44535 (EMBL:AL078618) putative acetyltransferase from Streptomyces coelicolor (187 aa) fasta scores; opt: 215, z-score: 271.9, E(): 8.8e-08, (34.2% identity in 146 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 4 3 22 19 [Reporter]SCO7610 (4L18)_PCR/6023 [Gene]SCO7610/10967 NC_003888 SC2H2.08, possible transcriptional regulator, len: 371 aa; similar to many other Streptomyces coelicolor putative transcriptional regulators, e.g. TR:Q9KYU5 (EMBL:AL355832) putative DeoR-family transcriptional regulator SCE22.17, 323 aa; fasta scores: opt: 458 z-score: 518.3 E(): 2.4e-21; 32.3% identity in 319 aa overlap. Contains possible helix-turn-helix motif at residues 24..45 (+3.61 SD) 1 3 11 6 [Reporter]SCO2899 (16I18)_PCR/4257 [Gene]SCO2899/7747 NC_003888 SCE6.36c, possible GroES-family molecular chaperone, len: 110 aa. Similar to many members of the GroES chaperonin family including: Rhizobium meliloti SW:CH13_RHIME(EMBL:M94191) 10 KD chaperonin C (GroES-C) (101 aa), fasta scores opt: 172 z-score: 231.3 E(): 1.9e-05 33.7% identity in 101 aa overlap. Contains 2xPfam matches to entry PF00166 cpn10, Chaperonins 10 Kd subunit. 4 3 22 18 [Reporter]SCO6829 (5H18)_PCR/6022 [Gene]SCO6829/10965 NC_003888 SC4A9.06c, possible oxidoreductase, len: 259 aa. Highly similar to Streptomyces coelicolor TR:Q9X890(EMBL:AL049707) putative oxidoreductase, SCE15.15 (260 aa), fasta scores opt: 1088 z-score: 1221.9 E(): 0 66.9% identity in 254 aa overlap. Also similar to Streptomyces griseus subsp. griseus TR:Q9XDF5(EMBL:AF074603) putative ketoacyl reductase (271 aa), fasta scores opt: 421 z-score: 479.1 E(): 2.8e-19 36.7% identity in 256 aa overlap. Contains a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature and a Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 1 3 11 5 [Reporter]SCO3750 (17E18)_PCR/4256 [Gene]SCO3750/7745 NC_003888 SCH22A.28c, possible two-component sensor histidine kinase, len: 416 aa; similar to SW:UHPB_SALTY (EMBL:M89480) Salmonella typhimurium sensor protein UhpB (EC 2.7.3.-), 500 aa; fasta scores: opt: 274 z-score: 299.1 E(): 3e-09; 26.4% identity in 337 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 22 17 [Reporter]SCO7554 (6D18)_PCR/6021 [Gene]SCO7554/10963 NC_003888 SC5F1.08c, probable lacI-family transcriptional regulator, len: 337 aa; similar to TR:Q9X9R3 (EMBL:AJ009798) Streptomyces reticuli CebR protein, 350 aa; fasta scores: opt: 1152 z-score: 1278.6 E(): 0; 54.6% identity in 339 aa overlap and to SW:CELR_THEFU (EMBL:AF086819) Thermomonospora fusca transcription regulator CelR, 340 aa; fasta scores: opt: 1031 z-score: 1145.4 E(): 0; 52.1% identity in 340 aa overlap. Also similar to TR:CAC10103 (EMBL:AL442165) Streptomyces coelicolor lacI-family transcriptional regulatory protein CebR 2SCC13.02c, 351 aa; fasta scores: opt: 1082 z-score: 996.3 E(): 0; 52.8% identity in 339 aa overlap. Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family. Also contains possible helix-turn-helix motif at residues 7..28 (+4.69 SD) 1 3 11 4 [Reporter]SCO1512 (18A18)_PCR/4255 [Gene]SCO1512/7743 NC_003888 SCL2.02, unknown, len: 46 aa 4 3 22 16 [Reporter]SCO1411 (7P14)_PCR/6020 [Gene]SCO1411/10961 NC_003888 SC6D7.28c, possible transmembrane transport protein, len: 440 aa. Highly similar to many other membrane transport proteins e.g. Escherichia coli SW:CIT1_ECOLI(EMBL:M11559) citrate-proton symporter (citrate transporter) (431 aa), fasta scores opt: 349 z-score: 368.9 E(): 3.6e-13 25.1% identity in 438 aa overlap and Yersinia pestis TR:Q9ZC42(EMBL:AL031866) putative transport protein (409 aa), fasta scores opt: 1363 z-score: 1421.9 E():0 49.5% identity in 410 aa overlap. Contains a Prosite hit to PS00217 Sugar transport proteins signature 2 and a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter. Also contains multiple possible membrane spanning hydrophobic domains. 1 3 11 3 [Reporter]SCO4880 (19M14)_PCR/4254 [Gene]SCO4880/7741 NC_003888 2SCK8.06, possible transferase, len: 424 aa; similar to TR:O88719 (EMBL:AJ006215) Mus musculus CMP-N-acetylneuraminic acid synthetase (EC 2.7.7.43) CmaS, 432 aa; fasta scores: opt: 361 z-score: 400.1 E(): 1e-14; 30.3% identity in 389 aa overlap. Contains Pfam matches to entries PF02348 Cytidylyl_trans, Cytidylyl transferase and PF00702 Hydrolase, haloacid dehalogenase-like hydrolase 4 1 16 12 [Reporter]SCO2550 (11H8)_PCR/1853 [Gene]SCO2550/3389 NC_003888 SCC77.17c, possible lipoprotein, len: 171 aa. Contains an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. 1 3 11 2 [Reporter]SCO3988 (20E14)_PCR/4253 [Gene]SCO3988/7739 NC_003888 SCBAC25E3.25, unknown, len: 115 aa: no significant database matches. 4 3 22 14 [Reporter]SCO7530 (9H14)_PCR/6018 [Gene]SCO7530/10957 NC_003888 SC8G12.06c, possible regulatory protein, len: 360 aa. The N-terminus of this protein is similar to many regulatory proteins e.g. Deinococcus radiodurans TR:AAF11989(EMBL:AE002074) transcriptional regulator, MerR family (280 aa), fasta scores opt: 324 z-score: 367.6 E(): 5.1e-13 37.3% identity in 244 aa overlap. The C-terminus of this protein is similar to many hypothetical proteins and putative phosphatases e.g. Mycobacterium leprae TR:Q50188(EMBL:Z70722) putative phosphoprotein phosphatase (509 aa), fasta scores opt: 709 z-score: 790.9 E(): 0 54.6% identity in 238 aa overlap. Contains a Prosite hit to PS00552 Bacterial regulatory proteins, merR family signature and a Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family. 1 3 10 22 [Reporter]SCO5275 (1E10)_PCR/4251 [Gene]SCO5275/7737 NC_003888 2SC7G11.37, possible ATP/GTP binding protein, len: 1007 aa; similar to (EMBL:AL391588) Streptomyces coelicolor SCCB12.04, 1148 aa; fasta scores: opt: 4019 z-score: 3915.8 E(): 0; 62.6% identity in 994 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 3 22 13 [Reporter]SCO1933 (10D14)_PCR/6017 [Gene]SCO1933/10955 NC_003888 SCC22.15c, unknown, len: 144aa; 1 3 10 21 [Reporter]SCO4184 (2A10)_PCR/4250 [Gene]SCO4184/7735 NC_003888 SCD66.21, amfC, aerial mycelium formation, len: 222 aa; identical to previously sequenced TR:Q54191 (EMBL:D63677) Streptomyces coelicolor aerial micelium formation AmfC, 222 aa 4 3 22 12 [Reporter]SCO4335 (11P10)_PCR/6016 [Gene]SCO4335/10953 NC_003888 SCD12A.18c, unknown, len: 62 aa 1 3 10 20 [Reporter]SCO4708 (3M6)_PCR/4249 [Gene]SCO4708/7733 NC_003888 SCD31.33, rpsC, 30S ribosomal protein S3, len: 277 aa; hihgly similar to SW:RS3_BACSU (EMBL:D50302) Bacillus subtilis 30S ribosomal protein S3 RpsC, 217 aa; fasta scores: opt: 801 z-score: 820.5 E(): 0; 55.1% identity in 205 aa overlap. Contains Pfam matches to entries PF00417 Ribosomal_S3_N, Ribosomal protein S3, N-terminal domain, PF00013 KH-domain, KH domain and PF00189 Ribosomal_S3_C, Ribosomal protein S3, C-terminal domain and matches to Prosite entries PS00050 Ribosomal protein L23 signature and PS00548 Ribosomal protein S3 signature 4 3 22 11 [Reporter]SCO4150 (12L10)_PCR/6015 [Gene]SCO4150/10951 NC_003888 SCD84.17, possible ABC transport system transmembrane protein, len: 206 aa; similar to TR:O69958 (EMBL:AL022268) Streptomyces coelicolor putative transmembrane transport protein SC4H2.03c, 411 aa; fasta scores: opt: 136 z-score: 161.5 E(): 0.16; 30.8% identity in 185 aa overlap. Contains possible hydrophobic membrane sapnning regions 1 3 10 19 [Reporter]SCO6622 (4I6)_PCR/4248 [Gene]SCO6622/7731 NC_003888 SC1F2.19, probable ATP/GTP binding protein, len: 628 aa; very weak similarity in C-terminus to a yeast ATP /GTP binding protein TR:Q12179 (EMBL:Z73601) Saccharomyces cerevisiae chromosome XVI ORF YPL245W (454 aa), fasta scores; opt: 201 z-score: 198.6 E(): 0.00081, 25.2% identity in 469 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop) and PS00152 ATP synthase alpha and beta subunits signature. Also similar to downstream gene SC1F2.20, E(): 0, 37.1% identity in 657 aa overlap 4 1 16 6 [Reporter]SCO0265 (16P24)_PCR/1847 [Gene]SCO0265/3379 NC_003888 SCF1.07, possible hydroxylase, len: 263 aa; similar to many from Streptomyces sp. eg. TR:Q55078 (EMBL:U50973) daunomycin C-14 hydroxylase from Streptomyces sp. strain C5 (275 aa) fasta scores; opt: 302, z-score: 356.8, E(): 1.7e-12, (39.3% identity in 270 aa overlap). Also similar to TR:CAB45588 (EMBL:AL079355) putative hydroxylase from Streptomyces coelicolor (263 aa) fasta scores; opt: 746, z-score: 870.2, E(): 0, (44.3% identity in 262 aa overlap). 1 3 10 18 [Reporter]SCO5724 (5E6)_PCR/4247 [Gene]SCO5724/7729 NC_003888 SC3C3.10, unknown, len: 124 aa 4 3 22 9 [Reporter]SCO0608 (14D10)_PCR/6013 [Gene]SCO0608/10947 NC_003888 StF55.32, possible regulatory protein, len: 292 aa; similar to SW:ALGP_PSEAE (EMBL:M30145) Pseudomonas aeruginosa transcriptional regulatory protein (alginate regulatory protein) AlgP, 340 aa; fasta scores: opt: 239 z-score: 217.9 E(): 9.1e-05; 35.8% identity in 193 aa overlap. Contains Lysine/Alanine rich region 1 3 10 17 [Reporter]SCO2040 (6A6)_PCR/4246 [Gene]SCO2040/7727 NC_003888 SC4G6.09c, possible membrane protein, len: aa; similar to TR:O06133 (EMBL:Z95554) hypothetical protein from Mycobacterium tuberculosis (132 aa) fasta scores; opt: 245, z-score: 301.1, E(): 1.9e-09, (36.7% identity in 128 aa overlap). Contains possible membrane spanning hydrophobic regions 4 3 22 8 [Reporter]SCO2966 (15P6)_PCR/6012 [Gene]SCO2966/10945 NC_003888 SCE59.25c, smpB, small protein B homologue, len: 159 aa; highly similar to SW:SMPB_ENTFA (EMBL:M90060) Enterococcus faecalis small protein B homologue SmpB, 154 aa; fasta scores: opt: 559 z-score: 693.2 E(): 3.3e-31; 56.1% identity in 155 aa overlap. Contains Pfam match to entry PF01668 SmpB, SmpB protein and match to Prosite entry PS01317 Protein smpB signature 1 3 10 16 [Reporter]SCO3606 (7M2)_PCR/4245 [Gene]SCO3606/7725 NC_003888 SC66T3.17, possible regulator, len: 360 aa; similar to the C-terminal region of TR:Q00509 (EMBL:X63451), SrmR, Streptomyces ambofaciens polyketide synthase regulatory gene (604 aa), fasta scores; opt: 248 z-score: 291.7 E(): 6.4e-09, 25.3% identity in 372 aa overlap. Also similar to the C-terminal region of Mycobacteria e.g. TR:O69477 (EMBL:AL023635) Mycobacterium leprae hypothetical protein (414 aa) (26.0% identity in 277 aa overlap). Weakly similar to the C-terminal region of SC8D9.30c (EMBL:AL035569) S.coelicolor possible transcriptional regulator (415 aa) (26.7% identity in 303 aa overlap). Contains probable helix-turn-helix motif at aa 237-258 (score 1012, +2.63 SD) 4 3 22 7 [Reporter]SCO0461 (16L6)_PCR/6011 [Gene]SCO0461/10943 NC_003888 SCF76.01, possible hydrolase, partial CDS, len: >228 aa. Similar to several hypothetical proteins e.g. from Saccharomyces cerevisiae SW:YN93_YEAST (EMBLL:Z71679) (290 aa), fasta scores opt: 609 z-score: 749.0 E():0 42.4% identity in 229 aa overlap. Also weakly similar to Xanthobacter autotrophicus SW:HALO_XANAU (EMBL:M26950) haloalkane dehalogenase (EC 3.8.1.5) (310 aa), fasta scores opt: 151 z-score: 190.4 E():0.003 22.9% identity in 231 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold,SCF51A.39, possible hydrolase, partial CDS, len: >113 aa. Highly similar to Saccharomyces cerevisiae (Bakers yeast) SW:YN93_YEAST (EMBL; Z71679) hypothetical 32.8 KD protein in BIO3-HXT17 intergenic region. In addition to being similar to many dehalogenases e.g. Mycobacterium tuberculosis SW:YU14_MYCTU (EMBL; Z77163) putative haloalkane dehalogenase (EC 3.8.1.5) (300 aa), fasta scores opt: 222 z-score: 292.5 E(): 6.2e-09 37.0% identity in 100 aa overlap 1 3 10 15 [Reporter]SCO5566 (8I2)_PCR/4244 [Gene]SCO5566/7723 NC_003888 SC7A1.10, recG, ATP-dependent DNA helicase, len: 742aa; similar to many eg. SW:RECG_ECOLI ATP-dependent DNA helicase from Escherichia coli (693 aa) fasta scores; opt: 438, z-score: 1301.5, E(): 0, (35.7% identity in 723 aa overlap). Contains PS00017 ATP /GTP-binding site motif A (P-loop) and Pfam match to entry PF00271 helicase_C, Helicases conserved C-terminal domain, score 67.50, E-value 2.8e-16. 4 3 22 6 [Reporter]SCO3567 (17H6)_PCR/6010 [Gene]SCO3567/10941 NC_003888 SCH17.01c, probable serine protease, partial CDS, len: >54 aa; continues as SCH5.30c in cosmid H5 (EMBL:AL035636). Alternative start codons are present at codons 3 and 8,SCH5.30c, partial CDS, probable serine protease, len: >385aa; similar to many eg. TR:O69639 (EMBL:AL022121) proposed serine protease from Mycobacterium tuberculosis (397 aa) fasta scores; opt: 835, z-score: 938.4, E(): 0, (39.9% identity in 386 aa overlap) and TR:Q44597 (EMBL:U07352) htrA, serine protease from Brucella abortus (513 aa) fasta scores; opt: 222, z-score: 251.4, E(): 1.1e-06, (30.6% identity in 180 aa overlap). 1 3 10 14 [Reporter]SCO7139 (9E2)_PCR/4243 [Gene]SCO7139/7721 NC_003888 SC8B1.03, probable aldehyde dehydrogenase (fragment), len: >469 aa; similar to SW:DHAB_RHIME (EMBL:U39940) Rhizobium meliloti betaine aldehyde dehydrogenase (EC 1.2.1.8) BetB, 487 aa; fasta scores: opt: 1021 z-score: 1101.5 E(): 0; 40.5% identity in 462 aa overlap and to TR:CAB46804 (EMBL:AL096811) Streptomyces coelicolor putative aldehyde dehydrogenase SCI30A.27c, 462 aa; fasta scores: opt: 1074 z-score: 994.9 E(): 0; 52.9% identity in 448 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family, and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00687 Aldehyde dehydrogenases glutamic acid active site,SC9A4.01c, possible dehydrogenase (fragment), len: >51 aa; highly similar to C-terminal region of SW:DHAB_BACSU (EMBL:U47861) Bacillus subtilis betaine aldehyde dehydrogenase (EC 1.2.1.8) gbsA, 490 aa; fasta scores: opt: 179 z-score: 258.5 E(): 6.3e-07; 56.0% identity in 50 aa overlap 4 1 15 21 [Reporter]SCO2198 (2H16)_PCR/1840 [Gene]SCO2198/3369 NC_003888 SC3H12.06, glnA, glutamine synthetase I, len: 469 aa; identical to previously sequenced SW:GLNA_STRCO (EMBL:M23172) Streptomyces coelicolor glutamine synthetase (EC 6.3.1.2) (glutamate-ammonia ligase) GlnA, 469 aa and to previously sequenced TR:CAB51282 (EMBL:AL096872) Streptomyces coelicolor glutamine synthetase I SC5F7.03, 469 aa and highly similar to SW:GLN1_STRVR (EMBL:X70924) Streptomyces viridochromogenes glutamine synthetase I (EC 6.3.1.2) (glutamate-ammonia ligase I) GlnI or GlnA, 469 aa; fasta scores: opt: 2905 z-score: 3417.5 E(): 0; 90.6% identity in 469 aa overlap. Contains Pfam match to entry PF00120 gln-synt, Glutamine synthetase and three Prosite matches to entries PS00180 Glutamine synthetase signature 1, PS00181 Glutamine synthetase putative ATP-binding region signature and PS00182 Glutamine synthetase class-I adenylation site 1 3 10 13 [Reporter]SCO6482 (10A2)_PCR/4242 [Gene]SCO6482/7719 NC_003888 SC9C7.18, conserved hypothetical protein, len: 348 aa; similar to two hypothetical proteins e.g. TR:O69685 (EMBL:AL022121) Mycobacterium tuberculosis RV03718c or MTV025.066c, 147 aa; fasta scores; opt: 475, z-score: 768.3, E(): 0, (52.1% identity in 146 aa overlap) 4 3 22 4 [Reporter]SCP1.281 (19P2)_PCR/6008 [Gene]SCP1.281/10937 NC_003888 SCP1.281, unknown, len: 115aa; 1 3 10 12 [Reporter]SCO2764 (10M22)_PCR/4241 [Gene]SCO2764/7717 NC_003888 SCC57A.35c, possible lipoprotein, len: 336 aa. Contains an appropriately positioned Prosite hit to PS00013 Prokaryotic membrane lipoprotein lipid attachment site and a possible N-terminal signal sequence. 4 3 22 3 [Reporter]SCP1.143 (20L2)_PCR/6007 [Gene]SCP1.143/10935 NC_003888 SCP1.143, possible membrane protein, len: 369aa; contains one possible membrane-spanning hydrophobic region. 1 3 10 11 [Reporter]SCO4675 (11I22)_PCR/4240 [Gene]SCO4675/7715 NC_003888 SCD40A.21c, hypothetical protein, len: 302 aa; similar to TR:Q9XD94 (EMBL:AF106004) Streptomyces coelicolor hypothetical 34.1 kD protein, 304 aa; fasta scores: opt: 850 z-score: 992.1 E(): 0; 45.0% identity in 300 aa overlap. Contains match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. 4 3 21 22 [Reporter]SCO1146 (1D22)_PCR/6004 [Gene]SCO1146/10933 NC_003888 2SCG38.39c, possible lipoprotein, len: 252 aa; similar to TR:Q9RK82 (EMBL:AL132662) Streptomyces coelicolor hypothetical 26.4 kDa protein SCF11.08, 250 aa; fasta scores: opt: 645 z-score: 660.7 E(): 2.4e-29; 42.1% identity in 247 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 1 3 10 10 [Reporter]SCO3386 (12E22)_PCR/4239 [Gene]SCO3386/7713 NC_003888 SCE126.04, unknown, len: 90aa; 4 3 21 21 [Reporter]SCO1821 (2P18)_PCR/6003 [Gene]SCO1821/10931 NC_003888 SCI8.06c, moaA, molybdenum cofactor biosynthesis protein A, len: 341 aa. Highly similar to many molybdenum cofactor biosynthesis protein A's e.g. Mycobacterium tuberculosis TR:O53881 (EMBL:AL022004) MoaA-2 (360 aa), fasta scores opt: 1336 z-score: 1559.3 E(): 0 61.7% identity in 332 aa overlap and Staphylococcus carnosus TR:Q9ZIM6 (EMBL: AF022796) MoaA (340 aa), fasta scores opt: 627 z-score: 735.1 E():0 33.3% identity in 321 aa overlap. Contains Pfam match to entry PF01444 MoaA_NifB_PqqE, moaA / nifB / pqqE family and a Prosite hit to PS01305 moaA / nifB / pqqE family signature. 1 3 10 9 [Reporter]SCO0333 (13A22)_PCR/4238 [Gene]SCO0333/7711 NC_003888 SCF12.12c, possible dioxygenase (putative secreted protein), len: 503 aa; similar to many of undefined function and to TR:Q53353 (EMBL:S65040) lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (485 aa) fasta scores; opt: 529, z-score: 596.3, E(): 7.3e-26, (31.6% identity in 493 aa overlap) 4 1 15 16 [Reporter]SCO5706 (7D12)_PCR/1835 [Gene]SCO5706/3359 NC_003888 SC9F2.10c, probable translational initiation factor, partial CDS, len: >249aa; similar to the C-terminal region of many eg. SW:IF2_BACST translational initiation factor IF2 from Bacillus stearothermophilus (741 aa) fasta scores; opt: 788, z-score: 893.1, E(): 0, (51.2% identity in 248 aa overlap).,SC5H4.30, probable translation initiation factor IF-2 (fragment), len: >835 aa; highly similar to SW:IF2_MYCLE (EMBL:AL035472) Mycobacterium leprae translation initiation factor IF-2 InfB, 924 aa; fasta scores: opt: 2559 z-score: 1220.5 E(): 0; 59.1% identity in 838 aa overlap and to SW:IF2_STIAU (EMBL:X87940) Stigmatella aurantiaca translation initiation factor IF-2 InfB, 1054 aa; fasta scores: opt: 1337 z-score: 648.2 E(): 1.2e-28; 38.8% identity in 891 aa overlap. Contains Pfam match to entry PF00009 GTP_EFTU, Elongation factor Tu family and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 10 8 [Reporter]SCO0254 (14M18)_PCR/4237 [Gene]SCO0254/7709 NC_003888 SCJ9A.33c, hypothetical protein, len: 246 aa; weakly similar to TR:Q18926 (EMBL:U23517) hypothetical protein from the eukaryote Caenorhabditis elegans (243 aa) fasta scores; opt: 354, z-score: 396.7, E(): 9.9e-15, (28.3% identity in 240 aa overlap). Also weakly similar to TR:O87611 (EMBL:AF087482) dienelactone hydrolase from Pseudomonas aeruginosa (236 aa) fasta scores; opt: 212, z-score: 241.9, E(): 4.1e-06, (27.4% identity in 230 aa overlap). Similar also to TR:Q9I4H1 (EMBL:AE004547) Pseudomonas aeruginosa hypothetical protein PA1166, 262 aa; fasta scores: opt: 474 Z-score: 515.3 E(): 4.5e-21; 35.000% identity in 240 aa overlap 4 3 21 19 [Reporter]SCO5610 (4H18)_PCR/6001 [Gene]SCO5610/10927 NC_003888 SC2E1.27c, unknown prophage gene, len: 120 aa; contains hydrophobic region near centre. Contains possible hydrophobic membrane spanning region 1 3 10 7 [Reporter]SCO0536 (15I18)_PCR/4236 [Gene]SCO0536/7707 NC_003888 SCF11.16c, unknown, len: 73 aa 4 3 21 18 [Reporter]SCO6825 (5D18)_PCR/6000 [Gene]SCO6825/10925 NC_003888 SC4A9.02c, possible integral membrane protein, len: 231 aa. Highly to several proteins of undefined function including Escherichia coli SW:DEDA_ECOLI (EMBL:M68934) DedA hypothetical protein found in the hisT-purF region (219 aa), fasta scores opt: 550 z-score: 642.2 E(): 2.3e-28 43.8% identity in 185 aa overlap and Streptomyces coelicolor TR:Q9X8J1 (EMBL:AL049841) possible membrane protein, SCE9.18c (303 aa), fasta scores opt: 812 z-score: 940.8 E():0 53.7% identity in 227 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a Pfam match to entry PF00597 DedA, DedA family. 1 3 10 6 [Reporter]SCO0814 (16E18)_PCR/4235 [Gene]SCO0814/7705 NC_003888 SCF43A.04, probable rhamnose kinase, len: 484 aa; similar to rhamnose kinases e.g. SW:RHAB_ECOLI (EMBL:L19201), rhaB, Escherichia coli rhamnulokinase (489 aa), fasta scores; opt: 1136 z-score: 1280.7 E(): 0, 41.5% identity in 468 aa overlap. Also similar to many other carbohydrate kinases e.g. SW:FUCK_ECOLI (EMBL:X15025), fucK, Escherichia coli L-fuculokinase (482 aa) (27.7% identity in 411 aa overlap). Contains Pfam match to entry PF00370 FGGY, FGGY family of carbohydrate kinases 4 3 21 17 [Reporter]SCO2193 (6P14)_PCR/5999 [Gene]SCO2193/10923 NC_003888 SC5F7.08, lipB, probable lipoate-protein ligase, len: 265aa; similar to many eg. SW:LIPB_MYCTU probable lipoate-protein ligase from Mycobacterium tuberculosis (230 aa) fasta scores; opt: 559, z-score: 652.0, E(): 5.5e-29, (52.0% identity in 223 aa overlap) and SW:LIPB_ECOLI lipoate-protein ligase B from Escherichia coli (191 aa) fasta scores; opt: 145, z-score: 177.0, E(): 0.016, (28.9% identity in 225 aa overlap). Contains PS01313 Lipoate-protein ligase B signature. 1 3 10 5 [Reporter]SCO3907 (17A18)_PCR/4234 [Gene]SCO3907/7703 NC_003888 SCH24.29, ssb, probable single-strand DNA-binding protein, len: 199 aa; similar to SW:SSB_SERMA (EMBL:X65080), Ssb, Serratia marcescens single-strand binding protein (175 aa), fasta scores; opt: 278 z-score: 245.3 E(): 2.4e-06, 33.3% identity in 168 aa overlap. C-terminus contains glycine-rich repeats similar to those found in eukaryotic RNA-binding proteins e.g. SW:GRP2_SINAL (EMBL:L31377), Grp2A, Sinapis alba (white mustard) glycine-rich RNA-binding protein (169 aa). Contains Pfam match to entry PF00436 SSB, Single-strand binding protein family, score 143.30, E-value 2.5e-39. 4 3 21 16 [Reporter]SCO1415 (7L14)_PCR/5998 [Gene]SCO1415/10921 NC_003888 SC6D7.24c, possible membrane protein, len: 582 aa. Highly similar to the Streptomyces coelicolor hypothetical protein SCF37.26c (64 aa), fasta scores opt: 207 z-score: 284.7 E(): 1.7e-10 52.4% identity in 63 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 10 3 [Reporter]SCO4021 (19I14)_PCR/4232 [Gene]SCO4021/7701 NC_003888 2SC10A7.25, probable two component system histidine kinase, len: 524 aa; similar to SW:AFQ2_STRCO (EMBL:D10654) Streptomyces coelicolor sensor protein AfsQ2 (EC 2.7.3.-), 535 aa; fasta scores: opt: 481 z-score: 498.8 E(): 2.8e-20; 29.0% identity in 517 aa overlap. Contains Pfam match to entry PF00672 DUF5, HAMP domain and PF00512 signal, Histidine kinase. Also contains possible N-terminal region signal peptide sequence 4 1 15 10 [Reporter]SCO3439 (13L4)_PCR/1829 [Gene]SCO3439/3349 NC_003888 SCE36.06, unknown, len: 290aa; 4 3 21 8 [Reporter]SCO3486 (15L6)_PCR/5990 [Gene]SCO3486/10905 NC_003888 SCE65.22, probable aldehyde dehydrogenase, len: 492 aa; similar to SW:ALDA_ECOLI (EBML:M64541) Escherichia coli aldehyde dehydrogenase A (EC 1.2.1.22) aldA, 478 aa; fasta scores: opt: 1189 z-score: 1347.0 E(): 0; 39.1% identity in 473 aa overlap and to Streptomyces coelicolor SCI35.34c, 483 aa; fasta scores: opt: 1014 z-score: 940.7 E(): 0; 40.2% identity in 455 aa overlap. Contains match to Pfam entry PF00171 aldedh, Aldehyde dehydrogenase and two matches to Prosite entries PS00687 Aldehyde dehydrogenases glutamic acid active site PS00070 Aldehyde dehydrogenases cysteine active site 4 3 21 7 [Reporter]SCO3693 (16H6)_PCR/5989 [Gene]SCO3693/10903 NC_003888 SCH35.31c, unknown, len: 180aa; similar to TR:O33344 (EMBL:AL008883) hypothetical protein from Mycobacterium tuberculosis (194 aa) fasta scores; opt: 266, z-score: 329.4, E(): 5.1e-11, (34.6% identity in 179 aa overlap). Contains TTA codon, possible target for bldA regulation. 4 3 21 6 [Reporter]SCO1234 (17D6)_PCR/5988 [Gene]SCO1234/10901 NC_003888 2SCG1.09c, ureC, urease alpha subunit, len: 573 aa; similar to SW:URE1_MYCTU (EMBL:L41141) Mycobacterium tuberculosis urease alpha subunit (EC 3.5.1.5) UreC, 577 aa; fasta scores: opt: 2744 z-score: 2925.3 E(): 0; 67.8% identity in 577 aa overlap and to TR:CAA19974 (EMBL:AL031124) Streptomyces coelicolor putative urease alpha subunit SC1C2.07, 558 aa; fasta scores: opt: 1483 z-score: 1444.2 E(): 0; 46.1% identity in 570 aa overlap. Contains Pfam match to entry PF00449 urease, Urease and match to Prosite entry PS01120 Urease nickel ligands signature and PS00145 Urease active site 4 1 14 15 [Reporter]SCO2483 (8L8)_PCR/1812 [Gene]SCO2483/3319 NC_003888 SC7A8.22, possible secreted protein, len: 236 aa. Contains possible N-terminal region signal peptide sequence 4 1 14 14 [Reporter]SCO5492 (9H8)_PCR/1811 [Gene]SCO5492/3317 NC_003888 SC8D9.04, probable oxidoreductase, len: 232aa; similar to many eg. TR:Q53882 (EMBL:U43704) aklaviketone reductase from Streptomyces sp. strain C5 (251 aa) fasta scores; opt: 319, z-score: 361.3, E(): 8.1e-13, (33.3% identity in 237 aa overlap). Contains PS00061 Short-chain dehydrogenases/reductases family signature. Also contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase, score 129.20, E-value 7.5e-35. 4 1 14 13 [Reporter]SCO7354 (10D8)_PCR/1810 [Gene]SCO7354/3315 NC_003888 SC9H11.08, unknown, len: 802 aa. Similar to many proteins of undefined function e.g. Streptomyces coelicolor TR:CAB61537(EMBL:AL133171) hypothetical 89.7 kd protein, SCF81.26 (835 aa), fasta scores opt: 2446 z-score: 2641.1 E(): 0 53.0% identity in 800 aa overlap. Contains Pfam matches to 2xentry PF00989 PAS, PAS domain. 4 1 14 12 [Reporter]SCO5283 (11P4)_PCR/1809 [Gene]SCO5283/3313 NC_003888 SCCB12.07c, possible two-component system response regulator, len: 244 aa; similar to TR:Q9ZEP4 (EMBL:AJ131213) Streptomyces coelicolor response regulator CseB or SCE94.09, 234 aa; fasta scores: opt: 632 z-score: 771.1 E(): 0; 44.3% identity in 230 aa overlap and to SW:RESD_BACSU (EMBL:L09228), 240 aa; fasta scores: opt: 573 z-score: 699.9 E(): 1.6e-31; 39.7% identity in 234 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal 4 1 14 11 [Reporter]SCO3937 (12L4)_PCR/1808 [Gene]SCO3937/3311 NC_003888 SCD78.04c, possible integrase/ recombinase within putative integrated plasmid, len: 495 aa; similar to TR:Q37839 (EMBL:D38173) ORF469 protein (possible site-specific recombinase) from Actinophage R4 (469 aa), fasta scores; opt: 261 z-score: 320.1 E(): 1.4e-10, 29.1% identity in 306 aa overlap and TR:O06604 (EMBL:Z95586) MTCY336.18 (Rv1586c) possible integrase from M. tuberculosis integrated phage phiRv1 (469 aa), fasta scores; opt: 371 z-score: 270.8 E(): 7.8e-08, (26.6% identity in 489 aa overlap). Probable coiled-coil from 414 to 451 (38 residues) Max score: 1.608 (probability 0.99) 4 1 14 10 [Reporter]SCO3205 (13H4)_PCR/1807 [Gene]SCO3205/3309 NC_003888 SCE22.22, probable MarR-family transcriptional repressor, len: 163 aa; similar to TR:Q9X5U1 (EMBL:AF127374) Streptomyces lavendula repressor MmcW, 163 aa; fasta scores: opt: 724 z-score: 892.8 E(): 0; 65.4% identity in 162 aa overlap and to TR:CAB70635 (EMBL:AL137242) Streptomyces coelicolor putative transcriptional regulator SC8F4.09c, 158 aa; fasta scores: opt: 409 z-score: 459.2 E(): 4.8e-20; 43.2% identity in 146 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 4 1 14 9 [Reporter]SCO1076 (14D4)_PCR/1806 [Gene]SCO1076/3307 NC_003888 SCG22.22c, possible secreted protein, len: 64 aa. Contains possible N-terminal region signal peptide sequence 4 1 14 8 [Reporter]SCO3419 (14P24)_PCR/1805 [Gene]SCO3419/3305 NC_003888 SCE9.26c, hypothetical protein, len: 213 aa; unknown function, weakly similar to TR:P7170 (EMBL:Z80775) Mycobacterium tuberculosis hypothetical protein (180 aa), fasta scores; opt: 154 z-score: 195.5 E(): 0.0014, 27.0% identity in 178 aa overlap. The start codon is uncertain as there are 2 potential 4 1 14 7 [Reporter]SCO1023 (15L24)_PCR/1804 [Gene]SCO1023/3303 NC_003888 SCG20A.03, possible membrane protein, len: 202 aa; similar to TR:Q9RIY8 (EMBL:AL109962) Streptomyces coelicolor hypothetical 20.8 kD protein, SCJ1.26, 193 aa; fasta scores: opt: 201 z-score: 245.7 E(): 3.3e-06; 35.4% identity in 195 aa overlap. Contains possible hydrophobic membrane spanning regions 4 1 14 6 [Reporter]SCO0754 (16H24)_PCR/1803 [Gene]SCO0754/3301 NC_003888 SCF81.13, putative secreted protein, len: 268 aa. Contains possible N-teminal signal sequence peptide 4 1 4 5 [Reporter]SCO0388 (17L12)_PCR/1582 [Gene]SCO0388/2899 NC_003888 SCF62.14, unknown, len: 653 aa. Weakly similar to Cytophaga heparina TR: Q59289 (EMBL; U27586) heparinase III protein precursor (EC 4.2.2.8) (659 aa), fasta scores opt: 202 z-score: 229.2 E(): 2.1e-05 22.8% identity in 421 aa overlap. 4 1 4 4 [Reporter]SCO2948 (18H12)_PCR/1581 [Gene]SCO2948/2897 NC_003888 SCE59.07c, hypothetical protein, len: 193 aa; similar to various hypothetical proteins, e.g. TR:P71608 (EMBL:Z80233) Mycobacterium tuberculosis hypothetical 21.8 kD protein CY10H4.38, 202 aa; fasta scores: opt: 525 z-score: 594.5 E(): 1.1e-25; 46.7% identity in 182 aa overlap 4 1 4 3 [Reporter]SCP1.80 (19D12)_PCR/1580 [Gene]SCP1.80/2895 NC_003888 SCP1.80, unknown, len: 333aa; full length similarity with TR:Q54402 (EMBL:Z11519) hypothetical protein from transposable element, Tn4811 of Streptomyces lividans 66 (336 aa) fasta scores; opt: 1080, z-score: 1214.2, E(): 0, 49.0% identity in 335 aa overlap. C-terminal similar to SCP1.40c/SCP1.314 (previously sequenced as TR:Q9L8H3 (EMBL:AF204735)) (87 aa) fasta scores; opt: 311, z-score: 364.2, E(): 8e-13, 50.0% identity in 82 aa overlap. 4 1 3 22 [Reporter]SCO4198 (1L4)_PCR/1577 [Gene]SCO4198/2893 NC_003888 2SCD46.12, probable DNA-binding protein, len: 141 aa; N-terminal region similar to many eg. TR:O53759 (EMBL:AL021933) putative regulatory protein from Mycobacterium tuberculosis (140 aa) fasta scores; opt: 412, z-score: 503.0, E(): 1.6e-20, 56.6% identity in 113 aa overlap. Contains Pfam match to entry PF01381 HTH_3, Helix-turn-helix and two helix-turn-helix motifs; (Score 979 (+2.52 SD)) at residue 51-72 and (Score 1963 (+5.87 SD)) at residue 22-43. 1 3 22 5 [Reporter]SCO1541 (18A6)_PCR/4498 [Gene]SCO1541/8199 NC_003888 SCL2.31, probable regulator, len: 159 aa; similar to various Streptomyces regulatory proteins, e.g. TR:CAB51005 (EMBL:AL096852) Streptomyces coelicolor putative regulator SCE19A.24, 142 aa; fasta scores: opt: 503 z-score: 661.7 E(): 1.8e-29; 57.9% identity in 133 aa overlap and TR:P95753 (EMBL:D50051) Streptomyces griseus SsgA, 136 aa; fasta scores: opt: 326 z-score: 433.9 E(): 8.9e-17; 43.1% identity in 130 aa overlap 1 3 22 4 [Reporter]SCP1.76c (19M2)_PCR/4497 [Gene]SCP1.76c/8197 NC_003888 SCP1.76c, unknown, len: 181aa; 1 3 22 3 [Reporter]SCO1316 (20I2)_PCR/4496 [Gene]SCO1316/8195 NC_003888 SCBAC36F5.27c, possible integral membrane protein, len: 211 aa; similar to SW:YQ37_MYCTU (EMBL:Z80225) Mycobacterium tuberculosis hypothetical 23.0 kDa protein RV2637, 218 aa; fasta scores: opt: 833 Z-score: 928.0 bits: 178.8 E(): 4.4e-44; 58.706% identity in 201 aa overlap. Contains Pfam match to entry PF00597 DedA, DedA family and match to Prosite entry PS00606 Beta-ketoacyl synthases active site. Also contains possible hydrophobic membrane spanning regions 1 3 21 22 [Reporter]SCO1143 (1A22)_PCR/4493 [Gene]SCO1143/8193 NC_003888 2SCG38.36c, conserved hypothetical protein, len: 253 aa; similar to TR:O66972 (EMBL:AE000707) Aquifex aeolicus hypothetical 38.3 kDa protein AQ_775, 326 aa; fasta scores: opt: 376 z-score: 435.0 E(): 8.8e-17; 32.8% identity in 235 aa overlap 1 3 21 21 [Reporter]SCO5006 (2M18)_PCR/4492 [Gene]SCO5006/8191 NC_003888 SCK15.08, minD1, possible septum site-determining protein, len: 418 aa; N-terminal domain similar to TR:Q9RZD9 (EMBL:AE001862) Deinococcus radiodurans DNA-binding response regulator DRA0010, 211 aa; blastp scores: Score = 103 (36.3 bits), Expect = 0.0045, Identities = 50/194 (25%), Positives = 85/194 (43%) and C-terminal domain highly similar to TR:Q9ZF15 (EMBL:AJ005576) Streptomyces griseus septum site-determining protein MinD protein, 396 aa; fasta scores: opt: 1230 z-score: 1302.8 E(): 0; 70.5% identity in 281 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00991 ParA, ParA family ATPase 1 3 21 20 [Reporter]SCO6419 (3I18)_PCR/4491 [Gene]SCO6419/8189 NC_003888 SC1A6.08c, unknown, len: 218 aa 1 3 21 19 [Reporter]SCO5607 (4E18)_PCR/4490 [Gene]SCO5607/8187 NC_003888 SC2E1.24, possible prophage transcriptional regulator, len:64 aa; similar to the N-terminus of several regulatory proteins e.g. Streptomyces phaeochromogenes plasmid pJV1 TR:Q54677 (EMBL:U23762) traR (245 aa), fasta scores; opt:137 z-score: 268.5 E(): 1e-07, 39.3% identity in 61 aa overlap. Contains Pfam match to entry gntR PF00392, Bacterial regulatory proteins, gntR family, score 43.92 1 3 21 18 [Reporter]SCO2411 (5A18)_PCR/4489 [Gene]SCO2411/8185 NC_003888 SC4A7.39c, possible membrane protein, len: 184 aa; similar to various hypothetical proteins, e.g. SW:Y07R_MYCTU (EMBL:Z75555) Mycobacterium tuberculosis hypothetical 28.3 kD protein, 261 aa; fasta scores: opt: 188 z-score: 223.5 E(): 4.6e-05; 28.7% identity in 164 aa overlap and to S/ coelicolor SC4A7.41c, >165 aa; fasta scores: opt: 240 z-score: 255.3 E(): 7.9e-09; 36.0% identity in 161 aa overlap. Contains possible hydrophobic membrane spanning region 1 3 21 17 [Reporter]SCO6750 (6M14)_PCR/4488 [Gene]SCO6750/8183 NC_003888 SC5F2A.33c, possible IPP isomerase, len: 197 aa; similar to many e.g. SW:BCH4_RHOCA bacteriochlorophyll synthase (176 aa) fasta scores; opt: 354, z-score: 431.9, E(): 9.6e-17, (42.0% identity in 157 aa overlap) and SW:IPPI_SCHPO isopentenyl diphosphate isomerase from Schizosaccharomyces pombe (227 aa) fasta scores; opt: 219, z-score: 270.0, E(): 9.9e-08, (36.0% identity in 178 aa overlap). 1 3 21 16 [Reporter]SCO1418 (7I14)_PCR/4487 [Gene]SCO1418/8181 NC_003888 SC6D7.21c, possible integral membrane protein, len: 230 aa. Similar to several proteins of undefined function e.g. Haemophilus influenzae SW:Y522_HAEIN (EMBL:U32734) hypothetical protein HI0522 (218 aa), fasta scores opt: 492 z-score: 566.3 E(): 3.6e-24 39.1% identity in 207 aa overlap and Bacillus subtilis SW:YYAS_BACSU(EMBL:D26185) hypothetical 22.0 KD protein (201 aa), fasta scores opt: 232 z-score: 273.7 E(): 7.2e-08 26.5% identity in 181 aa overlap. Contains a 3xSRQ 1x SGQ repeat region in the N-terminus 1 3 21 15 [Reporter]SCO5220 (8E14)_PCR/4486 [Gene]SCO5220/8179 NC_003888 SC7E4.17, unknown, len: 326 aa 1 4 10 10 [Reporter]SCO4314 (12E21)_PCR/6254 [Gene]SCO4314/11397 NC_003888 SCD95A.47, hypothetical protein, len: 227 aa; similar to TR:O53480 (EMBL:AL021899) Mycobacterium tuberculosis hypothetical 23.2 kD protein MTV018.23, 213 aa; fasta scores: opt: 178 z-score: 217.9 E(): 0.00011; 29.0% identity in 200 aa overlap 1 3 21 14 [Reporter]SCO5419 (9A14)_PCR/4485 [Gene]SCO5419/8177 NC_003888 SC8F4.23, trxA4, possible thioredoxin, len: 318aa; similar to many e.g. SW:P00275 (THI1_CORNE) thioredoxin C-1 from Corynebacterium nephridii (105 aa) fasta scores; opt: 297, z-score: 317.6, E(): 2.8e-10, 44.3% identity in 97 aa overlap. Contains Pfam match to entry PF00085 thiored, Thioredoxin and Prosite match to PS00194 Thioredoxin family active site. 1 4 10 9 [Reporter]SCO3752 (13A21)_PCR/6253 [Gene]SCO3752/11395 NC_003888 SCH22A.30, possible ABC transporter ATP-binding protein, len: 252 aa; similar to TR:Q9XAA7 (EMBL:AL096837) Streptomyces coelicolor putative ABC transporter ATP-binding protein SCF43A.08, 264 aa; fasta scores: opt: 848 z-score: 944.8 E(): 0; 56.0% identity in 243 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and match to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 3 21 13 [Reporter]SCO2570 (10M10)_PCR/4484 [Gene]SCO2570/8175 NC_003888 SCC123.08c, possible secreted protein, len: 258 aa. Contains a possible N-terminal signal sequence. 1 4 10 8 [Reporter]SCO0915 (14M17)_PCR/6252 [Gene]SCO0915/11393 NC_003888 SCM10.03, unknown, len: 85 aa; similar to TR:O53714 (EMBL:AL021931) Mycobacterium tuberculosis MTV036.14 possible protein transport protein, 71 aa; fasta scores: opt: 96 z-score: 153.6 E(): 0.36; 24.2% identity in 62 aa overlap 1 3 21 12 [Reporter]SCO4328 (11I10)_PCR/4483 [Gene]SCO4328/8173 NC_003888 SCD12A.11c, possible lipoprotein, len: 154 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 1 4 10 7 [Reporter]SCO3852 (15I17)_PCR/6251 [Gene]SCO3852/11391 NC_003888 SCH69.22c, putative membrane protein, len: 69 aa. Contains possible hydrophobic membrane spanning region 1 3 21 11 [Reporter]SCO4168 (12E10)_PCR/4482 [Gene]SCO4168/8171 NC_003888 SCD66.05, probable oxidoreductase, len: 275 aa; similar to TR:BAA95121 (EMBL:AB042262) Corynebacterium aquaticum levodione reductase Lvr, 267 aa; fasta scores: opt: 477 z-score: 478.6 E(): 3.5e-19; 37.3% identity in 260 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain dehydrogenases/reductases family signature 1 3 21 10 [Reporter]SCO3246 (13A10)_PCR/4481 [Gene]SCO3246/8169 NC_003888 SCE29.15c, fabH4, probable 3-oxoacyl-[acyl carrier protein] synthase III, len: aa; similar to many eg. SW:FABH_ECOLI 3-oxoacyl-[acyl carrier protein] synthase III, FabH, from Escherichia coli (317 aa) fasta scores; opt: 765, z-score: 862.6, E(): 0, (40.8% identity in 314 aa overlap). Also similar to two from Streptomyces coelicolor - TR:O54151 (EMBL:AL021409) (335 aa) fasta scores; opt: 694, z-score: 782.9, E(): 0, (53.5% identity in 331 aa overlap) and TR:E1359140 (EMBL:AL034443) (316 aa) fasta scores; opt: 685, z-score: 773.2, E(): 0, (40.6% identity in 313 aa overlap). 1 4 10 5 [Reporter]SCO0723 (17A17)_PCR/6249 [Gene]SCO0723/11387 NC_003888 3SC5B7.01, conserved hypothetical protein (fragment), len: >151 aa; similar to TR:Q9SA67 (EMBL:AC006550) Arabidopsis thaliana F10O3 protein, 270 aa; fasta scores: opt: 219 z-score: 240.7 E(): 7.1e-06; 30.4% identity in 125 aa overlap,SCF42.33, unknown, partial CDS, len: > 117 aa. Similar to a protein of undefined function from Schizosaccharomyces pombe (Fission yeast) TR:CAB61463(EMBL:AL133156) (235 aa), fasta scores opt: 241 z-score: 282.8 E(): 2.3e-08 37.0% identity in 119 aa overlap and Escherichia coli SW:YGGC_ECOLI(EMBL:X14436) (237 aa), fasta scores opt: 184 z-score: 219.2 E(): 8.1e-05 36.4% identity in 99 aa overlap. Contains a Prosite Hit to PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 21 9 [Reporter]SCO2770 (14M6)_PCR/4480 [Gene]SCO2770/8167 NC_003888 SCC105.01c, speB, agmatinase (fragment), len: >302 aa; highly similar to N-terminal region of SW:SPEB_STRCL (EMBL:L15470) Streptomyces clavuligerus possible agmatinase (EC 3.5.3.11) Pah, 313 aa; fasta scores: opt: 843 z-score: 968.6 E(): 0; 43.3% identity in 293 aa overlap and of SW:SPEB_ECOLI (EMBL:M32363) Escherichia coli agmatinase (EC 3.5.3.11), SpeB, 306 aa; fasta scores: opt: 473 z-score: 546.7 E(): 5.1e-23; 41.8% identity in 282 aa overlap. Contains Pfam match to entry PF00491 arginase, Arginase family and matches to Prosite entries PS00147 Arginase family signature 1, PS00148 Arginase family signature 2, PS00678 Trp-Asp (WD) repeats signature and PS01053 Arginase family signature 3,SCC57A.41c, possible agmatinase, partial CDS, len: >53 aa. Highly similar to Escherichia coli SW:SPEB_ECOLI(EMBL:M32363) agmatinase (EC 3.5.3.11) (306 aa), fasta scores opt: 145 z-score: 242.9 E(): 3.8e-06 49.0% identity in 49 aa overlap and Schizosaccharomyces pombe SW:SPEB_SCHPO(EMBL:Z68166) putative agmatinase precursor (EC 3.5.3.11) (394 aa), fasta scores opt: 159 z-score: 263.7 E(): 2.7e-07 55.6% identity in 45 aa overlap. 1 4 10 4 [Reporter]SCP1.117 (18M13)_PCR/6248 [Gene]SCP1.117/11385 NC_003888 SCP1.117, unknown, len: 251aa; 1 3 21 8 [Reporter]SCO3902 (15I6)_PCR/4479 [Gene]SCO3902/8165 NC_003888 SH24.24, probable integral membrane protein, len: 502 aa; unknown function, similar to putative transmembrane proteins from Mycobacteria e.g. TR:O53127 (EMBL:AL022118) Mycobacterium leprae putative transmembrane protein (564 aa), fasta scores; opt: 520 z-score: 575.6 E(): 9.5e-25, 38.4% identity in 490 aa overlap. Contains several hydrophobic, probable membrane-spanning regions 1 4 10 3 [Reporter]SCO4881 (19I13)_PCR/6247 [Gene]SCO4881/11383 NC_003888 2SCK8.07, possible polysaccharide biosynthesis related protein, len: 312 aa; similar to SW:SPSE_BACSU (EMBL:X73124) Bacillus subtilis spore coat polysaccharide biosynthesis protein SpsE, 373 aa; fasta scores: opt: 473 z-score: 554.3 E(): 2.6e-23; 32.2% identity in 295 aa overlap and to TR:Q9NR45 (EMBL:AF257466) Homo sapiens N-acetylneuraminic acid phosphate synthase, 359 aa; fasta scores: opt: 660 z-score: 771.2 E(): 0; 38.4% identity in 294 aa overlap. Contains matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00639 Eukaryotic thiol (cysteine) proteases histidine active site 1 3 21 7 [Reporter]SCO1691 (16E6)_PCR/4478 [Gene]SCO1691/8163 NC_003888 SCI30A.12c, possible tetR transcriptional regulator, len: 251 aa; similar to many e.g. SW:BM3R_BACME transcriptional repressor from Bacillus megaterium (192 aa) fasta scores; opt: 190, z-score: 227.2, E(): 2.5e-05, (26.7% identity in 176 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and Prosite match to PS01081 Bacterial regulatory proteins, tetR family signature. Contains possible helix-turn-helix motif (+3.97 SD) 87-108aa. 1 4 10 2 [Reporter]SCO3987 (20A13)_PCR/6246 [Gene]SCO3987/11381 NC_003888 SCBAC25E3.22, conserved hypothetical protein, len: 101 aa: similar to TR:Q9X8D6 (EMBL:AL049573) hypothetical protein SCE39.13c from Streptomyces coelicolor (99 aa) fasta scores; opt: 441, Z-score: 546.5, 67.742% identity (67.742% ungapped) in 93 aa overlap 1 3 21 6 [Reporter]SCO3359 (17A6)_PCR/4477 [Gene]SCO3359/8161 NC_003888 SCE94.10, cseC, sensory histidine kinase, len: 507 aa; previously sequenced therefore identical to TR:E1358528 (EMBL:AJ131213) CseC, sensory histidine kinase from Streptomyces coelicolor (507 aa) fasta scores; opt: 3298, z-score: 3217.4, E(): 0, (100.0% identity in 507 aa overlap). Also similar to many others eg. SW:AFQ2_STRCO sensor protein, part of a putative two-component regulatory system involved in secondary metabolism in Streptomyces coelicolor (535 aa) fasta scores; opt: 382, z-score: 378.4, E(): 9.2e-14, (29.8% identity in 413 aa overlap). Contains Pfam matches to entry PF00672 DUF5, Domain found in bacterial signal proteins and entry PF00512 signal, Signal carboxyl-terminal domain. 1 3 21 5 [Reporter]SCO3716 (18M2)_PCR/4476 [Gene]SCO3716/8159 NC_003888 SCH35.08, probable cation transport system component, len: 225 aa; similar to many e.g. SW:ATKc_ECOLI AtkC/KdpC potassium transporting ATPase A chain from Escherichia coli (190 aa) fasta scores; opt: 230, z-score: 264.8, E(): 2e-07, (35.0% identity in 203 aa overlap). Contains possible membrane spanning hydrophobic region 1 4 9 21 [Reporter]SCO2736 (2M5)_PCR/6243 [Gene]SCO2736/11377 NC_003888 SCC57A.07c, citA, citrate synthase, len: 429 aa. Previously sequenced and characterised: Streptomyces coelicolor TR:AAF14286(EMBL:AF181118) citrate synthase (citA). Contains a Prosite hit to PS00480 Citrate synthase signature and a Pfam match to entry PF00285 citrate_synt, Citrate synthase. 1 3 21 4 [Reporter]SCO5336 (19I2)_PCR/4475 [Gene]SCO5336/8157 NC_003888 SCBAC5H2.05c, possible mutT-like protein, len: 156 aa; similar to plasmid borne SW:MUTT_STRAM (EMBL:Z19590) Streptomyces ambofaciens plasmid pSAM2 mutT-like protein ORF154, 154 aa; fasta scores: opt: 182 z-score: 232.1 E(): 2.4e-05; 32.7% identity in 153 aa overlap. Contains Pfam match to entry PF00293 mutT, MutT-like domain, and match to Prosite entry PS00893 mutT domain signature 1 4 9 20 [Reporter]SCO4704 (3I5)_PCR/6242 [Gene]SCO4704/11375 NC_003888 SCD31.29, rplW, 50S ribosomal protein L23, len: 139 aa; highly similar to SW:RL23_BACSU (EMBL:D50302) Bacillus subtilis 50S ribosomal protein L23 RplW, 95 aa; fasta scores: opt: 241 z-score: 308.8 E(): 9e-10; 41.8% identity in 91 aa overlap. Contains Pfam match to entry PF00276 Ribosomal_L23, Ribosomal protein L23 and match to Prosite entry PS00050 Ribosomal protein L23 signature 1 3 21 3 [Reporter]SCO6103 (20E2)_PCR/4474 [Gene]SCO6103/8155 NC_003888 SCBAC1A6.27c, possible acetyltransferase, len: 190 aa. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 4 9 19 [Reporter]SCO6923 (4E5)_PCR/6241 [Gene]SCO6923/11373 NC_003888 SC1B2.29c, unknown, len: 238 aa. 1 3 21 2 [Reporter]SCO0975 (20M22)_PCR/4473 [Gene]SCO0975/8153 NC_003888 SCBAC19F3.02, zwf3, 6-phosphogluconate 1-dehydrogenase, len: 479 aa: similar to many eg. SW:P00350 (6PGD_ECOLI) 6-phosphogluconate dehydrogenase from Escherichia coli (468 aa) fasta scores; opt: 1627, Z-score: 1751.0, 53.433% identity (54.013% ungapped) in 466 aa overlap. Contains Pfam match to entry PF00393 6PGD, 6-phosphogluconate dehydrogenase. 1 4 9 18 [Reporter]SCO6362 (5A5)_PCR/6240 [Gene]SCO6362/11371 NC_003888 SC3A7.30, probable two-component sensor, len: 429 aa; similar to many e.g. UHPB_SALTY sensor protein UhpB (500 aa), fasta scores; opt: 238 z-score: 263.3 E(): 2.1e-07, 30.7% identity in 358 aa overlap. Contains six probable hydrophobic transmembrane domains in N-terminus 1 3 20 22 [Reporter]SCO1340 (1M18)_PCR/4471 [Gene]SCO1340/8151 NC_003888 2SCG61.22, conserved hypothetical protein, len: 170 aa; similar to others of unknown function from Streptomyces coelicolor e.g. TR:CAA20815 (EMBL:AL031541) hypothetical protein (175 aa) fasta scores; opt: 568, z-score: 738.0, E(): 0, 52.1% identity in 169 aa overlap, to SW:YM2_STRCO (EMBL:X15942) mini-circle hypothetical 13.3 kD protein (122 aa) fasta scores; opt: 366, z-score: 482.1, E(): 2.2e-19, 47.2% identity in 123 aa overlap and to TR:Q9AJE6 (EMBL:AB048795) Kitasatospora griseola hypothetical protein, 170 aa; fasta scores: opt: 614 Z-score: 754.9 E(): 2.1e-34; 56.140% identity in 171 aa overlap 1 3 20 21 [Reporter]SCO4958 (2I18)_PCR/4470 [Gene]SCO4958/8149 NC_003888 2SCK31.18, metB, cystathionine gamma-synthase, len: 386 aa; highly similar to TR:Q9RQ97 (EMBL:AF126953) Corynebacterium glutamicum cystathionine gamma-synthase MetB, 396 aa; fasta scores: opt: 1497 z-score: 1715.4 E(): 0; 59.2% identity in 377 aa overlap. Contains Pfam match to entry PF01053 Cys_Met_Meta_PP, Cys/Met metabolism PLP-dependent enzyme and match to Prosite entry PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1 1 4 9 16 [Reporter]SCO3594 (7I1)_PCR/6238 [Gene]SCO3594/11367 NC_003888 SC66T3.05, dldh, probable D-lactate dehydrogenase, len: 337 aa; highly similar to many e.g. TR:O33805 (EMBL:U8296) Streptomyces toyocaensis D-lactate dehydrogenase (330 aa), fasta scores; opt: 1470 z-score: 1711.8 E(): 0,71.4% identity in 318 aa overlap. Also similar to D-specific alpha-keto acid dehydrogenases from vancomycin-resistant bacteria e.g. SW:VANH_ENTFC (EMBL:M64304), VanH, Enterococcus faecium alpha-keto acid dehydrogenase (322 aa) (61.4% identity in 316 aa overlap). Similar to other S.coelicolor putative dehydrogenases e.g. part of SerA (EMBL:AL035569) S.coelicolor probable D-3-phosphoglycerate dehydrogenase (529 aa) (32.2% identity in 255 aa overlap). Contains Pfam matches to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases, PS00670 D-isomer specific 2-hydroxyacid dehydrogenases signature 2 and PS00671 D-isomer specific 2-hydroxyacid dehydrogenases signature 3 1 3 20 20 [Reporter]SCO7649 (3E18)_PCR/4469 [Gene]SCO7649/8147 NC_003888 SC10F4.22c, probable two-component system sensor kinase, len: 398 aa; similar to many other Streptomyces coelicolor sensor kinases, e.g. TR:Q9RKT9 (EMBL:AL132824) putative two-component sensor kinase SCAH10.18, 417 aa; fasta scores: opt: 780 z-score: 815.5 E(): 0; 44.0% identity in 348 aa overlap. Contains possible hydrophobic membrane spanning regions in the N-terminal domain 1 4 9 15 [Reporter]SCO2144 (8E1)_PCR/6237 [Gene]SCO2144/11365 NC_003888 SC6G10.17, possible integral membrane transporter, len: 469aa; similar to many eg. SW:CITT_ECOLI CitR, citrate transporter from Escherichia coli (487 aa) fasta scores; opt: 257, z-score: 284.2, E(): 1.6e-08, (23.3% identity in 454 aa overlap). Contains Pfam match to entry PF00939 Na_sulph_symp, Sodium:sulfate symporter transmembrane region, score -165.10, E-value 3.2e-06 and several probable membrane spanning hydrophobic regions. 1 3 20 19 [Reporter]SCO5603 (4A18)_PCR/4468 [Gene]SCO5603/8145 NC_003888 SC2E1.20, unknown ATP/GTP binding protein, len: 541 aa; similar to hypothetical proteins from many organisms e.g. YIFB_ECOLI (516 aa), fasta scores; opt: 356 z-score: 935.9 E(): 0, 37.0% identity in 513 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 4 9 14 [Reporter]SCO6595 (9A1)_PCR/6236 [Gene]SCO6595/11363 NC_003888 SC8A6.16, probable secreted protein, len: 380 aa; co ntains N-terminal signal sequence and TTA Leu codon, possib le target for action of bldA 1 3 20 18 [Reporter]SCO7676 (5M14)_PCR/4467 [Gene]SCO7676/8143 NC_003888 SC4C2.11, probable ferredoxin, len: 73 aa; similar to SW:FER2_STRGO (EMBL:M32239) Streptomyces griseolus ferredoxin 2 SubB, 64 aa; fasta scores: opt: 236 z-score: 326.6 E(): 1.2e-10; 55.7% identity in 61 aa overlap 1 4 9 13 [Reporter]SCO1477 (9M21)_PCR/6235 [Gene]SCO1477/11361 NC_003888 SC9C5.01c, probable flavoprotein homologue (partial), len: >375 aa; similar to SW:DFP_ECOLI (EMBL:L10328) Escherichia coli DNA/pantothenate metabolism flavoprotein Dfp, 430 aa; fasta scores: opt: 846 z-score: 937.1 E(): 0; 43.8% identity in 347 aa overlap,SCL6.34c, probable flavoprotein homologue (partial), len: >89 aa; highly similar to TR:Q9X4Q1 (EMBL:AF117274) Streptomyces spectabilis flavoprotein homolog Dfp (fragment), 174 aa; fasta scores: opt: 417 z-score: 566.0 E(): 4.1e-24; 76.4% identity in 89 aa overlap and to C-teminal region of SW:DFP_SYNY3 (EMBL:D90910) Synechocystis sp. DNA/pantothenate metabolism flavoprotein homolog Dfp, 402 aa; fasta scores: opt: 199 z-score: 272.3 E(): 9.6e-08; 33.7% identity in 89 aa overlap 1 3 20 17 [Reporter]SCO6746 (6I14)_PCR/4466 [Gene]SCO6746/8141 NC_003888 SC5F2A.29, possible transcriptional regulator, len: 325aa; similar to many eg. TR:O30507 (EMBL:AF008308) arginine regulatory protein ArgR, from Pseudomonas aeruginosa (328 aa) fasta scores; opt: 288, z-score: 331.0, E(): 4e-11, (24.7% identity in 308 aa overlap). Also similar to TR:Q43970 (EMBL:Z48959) AraC-like protein from Azorhizobium caulinodans (227 aa) fasta scores; opt: 605, z-score: 689.7, E(): 4.2e-31, (47.4% identity in 192 aa overlap). Contains Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. 1 3 20 16 [Reporter]SCO5633 (7E14)_PCR/4465 [Gene]SCO5633/8139 NC_003888 SC6A9.34, probable fusion protein partially within putative integrated plasmid, len: 259 aa; N-terminus is almost identical to TR:E1286466 (EMBL:AJ005260) plasmid transfer protein traSA from Streptomyces ambofaciens plasmid pSAM2 (415 aa); 96.2% identity in 369 aa overlap, and similar to other members of the spoIIIE /ftsK family e.g. SPI_STRAZ sporulation-regulatory protein (303 aa), fasta scores; opt: 608 z-score: 904.0 E(): 0, 57.0% identity in 235 aa overlap. C-terminus is similar to many integrase /recombinase proteins e.g. TR:O55248 (EMBL:AF030986) Mycobacterium phage MS6 integrase (372 aa), fasta scores; opt: 184 z-score: 323.4 E(): 8.9e-11, 26.4% identity in 364 aa overlap. N-terminus contains TTA Leu codon; possible target for action of bldA and PS00017 ATP /GTP-binding= Pfam match to entry PF00589 Phage_integrase, Phage integrase family, score 40.60, E-value 3.5e-08 1 4 9 11 [Reporter]SCO4076 (11E21)_PCR/6233 [Gene]SCO4076/11357 NC_003888 SCD25.12c, putative LSR2-like protein, len: 105 aa. Highly similar to Streptomyces coelicolor TR:CAB40875 (EMBL; AL049628) putative LSR2-like protein SCE94.26C (111 aa), fasta scores opt: 335 z-score: 425.4 E(): 2.4e-16 50.5% identity in 109 aa overlap and Mycobacterium leprae SW: LSR2_MYCLE (EMBL; M67510) LSR2 protein precursor (15 KD antigen) (112 aa), fasta scores opt: 287 z-score: 367.2 E(): 4.2e-13 46.8% identity in 111 aa overlap. 1 3 20 15 [Reporter]SCO5214 (8A14)_PCR/4464 [Gene]SCO5214/8137 NC_003888 SC7E4.11, hypothetical protein, len: 271 aa; similar to TR:O05872 (EMBL:Z95121) Mycobacterium tuberculosis hypothetical 27.6 kD protein MTCY20B11.01c, 252 aa; fasta scores: opt: 383 z-score: 460.8 E(): 3.4e-18; 39.6% identity in 283 aa overlap 1 4 9 10 [Reporter]SCO4310 (12A21)_PCR/6232 [Gene]SCO4310/11355 NC_003888 SCD95A.43, hypothetical protein, len: 98 aa; highly similar to TR:P96070 (EMBL:U09991) Streptomyces violaceus ISP5230 chloramphenicol resistance protein (CMLV) and chloramphenicol phosphotransferase genes, complete CDS (fragment), 89 aa; fasta scores: opt: 450 z-score: 589.5 E(): 2.3e-25; 74.1% identity in 85 aa overlap 1 3 20 14 [Reporter]SCO7004 (9M10)_PCR/4463 [Gene]SCO7004/8135 NC_003888 SC8F11.30, probable carbohydrate kinase,len: 479 aa. Highly similar to many e.g. Bacillus subtilis SW:GLPK_BACSU(EMBL:M34393) glycerol kinase (EC 2.7.1.30), GlpK (496 aa), fasta scores opt: 688 z-score: 766.4 E():0 35.1% identity in 496 aa overlap. Contains a Pfam match to entry PF00370 FGGY, FGGY family of carbohydrate kinases. 1 4 9 9 [Reporter]SCO3173 (13M17)_PCR/6231 [Gene]SCO3173/11353 NC_003888 SCE87.24, possible merR-family transcriptional regulator, len: 215 aa. Similar to two other putative transcriptional regulators from Streptomyces coelicolor TR:CAB40683 (EMBL:AL049587) SC5F2A.16C (251 aa), fasta scores opt: 210 z-score: 257.9 E(): 5.4e-07 32.3% identity in 217 aa overlap and TR:O86531 (EMBL:AL031124) SC1C2.31C (214 aa), fasta scores opt: 176 z-score: 218.8 E(): 8.2e-05 30.4% identity in 191 aa overlap. Contains a Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family with the putative helix-turn-helix motif situated between residues 8..29 (+3.90 SD). 1 3 20 13 [Reporter]SCO2566 (10I10)_PCR/4462 [Gene]SCO2566/8133 NC_003888 SCC123.04c, hypothetical protein, len: 293 aa. Similar in parts to several proteins of unknown function e.g. Streptomyces coelicolor TR:O86515(EMBL:AL031124) hypothetical 20.0 KD protein (185 aa), fasta scores: opt: 384 z-score: 456.7 E(): 4.7e-18 32.8% identity in 180 aa overlap 1 4 9 8 [Reporter]SCO0273 (14I17)_PCR/6230 [Gene]SCO0273/11351 NC_003888 SCF85.01, unknown, partial CDS, len: >264 aa. Weakly similar to several hypotheticals e.g. Mycobacterium tuberculosis TR:O53485 (EMBL; AL021899) lipoprotein component of sugar transport system (439 aa), fasta scores opt: 258 z-score: 307.2 E(): 9.3e-10 27.9% identity in 222 aa overlap and Thermotoga maritima TR:AAD35892 (EMBL; AE001748) sugar ABC transporter, periplasmic sugar-binding protein TM0810 (420 aa), fasta scores opt: 169 z-score: 203.2 E(): 0.00058 25.1% identity in 263 aa overlap.,SCF1.15, partial CDS, possible substrate binding protein (extracellular) (binding-protein-dependent transport), len: >221aa; similar to many the N-terminal region of undefined function eg. SW:YURO_BACSU hypothetical ABC transporter extracellular binding protein from Bacillus subtilis (422 aa) fasta scores; opt: 224, z-score: 276.0, E(): 5.2e-08, (26.1% identity in 161 aa overlap). Contains possible N-terminal signal sequence. 1 3 20 12 [Reporter]SCO2624 (11E10)_PCR/4461 [Gene]SCO2624/8131 NC_003888 SCC80.09c, possible integral membrane protein, len: 395 aa; similar to TR:Q9RL36 (EMBL:AL121596) Streptomyces coelicolor hypothetical 29.6 kDa protein SCF51A.29c, 284 aa; fasta scores: opt: 269 z-score: 309.0 E(): 1.1e-09; 31.0% identity in 258 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 20 11 [Reporter]SCO4481 (12A10)_PCR/4460 [Gene]SCO4481/8129 NC_003888 SCD69.01, probable serine/threonine protein kinase (fragment), len: >607 aa; similar to SW:AFSK_STRCO (EMBL:D15062) Streptomyces coelicolor serine/threonine protein kinase AfsK, 799 aa; fasta scores: opt: 649 z-score: 427.0 E(): 2.5e-16; 35.8% identity in 408 aa overlap also similar to S. coelicolor SCD69.08 and TR:Q9ZN80 (EMBL:AB021679) Streptomyces coelicolor serine/threonine protein kinase PkaG, 592 aa; fasta scores: opt: 715 z-score: 417.5 E(): 1e-17; 32.7% identity in 538 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and matches to Prosite entries PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature. Contains also a TTA leucine codon, possible target for bldA regulation,SCD65.24, probable serine/threonine protein kinase (fragment), len: >56 aa; similar to SW:AFSK_STRCO (EMBL:D15062) Streptomyces coelicolor serine/threonine protein kinase AfsK (EC 2.7.1.-), 799 aa; fasta scores: opt: 137 z-score: 184.3 E(): 0.0092; 52.4% identity in 42 aa overlap and to TR:CAB92109 (EMBL:AL356334) Streptomyces coelicolor putative serine/threonine protein kinase SCD69.08, 626 aa; fasta scores: opt: 316 z-score: 370.1 E(): 5.6e-15; 86.8% identity in 53 aa overlap. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and match to Prosite entry PS00107 Protein kinases ATP-binding region signature 1 4 9 6 [Reporter]SCO0500 (16A17)_PCR/6228 [Gene]SCO0500/11347 NC_003888 SCF34.19c, unknown, len: 79 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference, amino acid composition and the presence of a potential RBS 1 3 20 10 [Reporter]SCO3273 (13M6)_PCR/4459 [Gene]SCO3273/8127 NC_003888 SCE39.23, unknown, len: 88aa; contains possible coiled-coil. 1 4 9 5 [Reporter]SCO4874 (17M13)_PCR/6227 [Gene]SCO4874/11345 NC_003888 SCK20.15, possible integral membrane protein, len: 961 aa. Contains match to Prosite entry PS01125 ROK family signature. Also contains 4x degenerate repeat: DRA(S/A)G and possible hydrophobic membrane spanning regions 1 3 20 9 [Reporter]SCO3909 (14I6)_PCR/4458 [Gene]SCO3909/8125 NC_003888 SCH24.31, rplI, 50S ribosomal protein L9, len: 148 aa; similar to many e.g. SW:RL9_BACST (EMBL:X62002), RplI, Bacillus stearothermophilus 50S ribosomal protein L9 (149 aa), fasta scores; opt: 346 z-score: 415.7 E(): 7.7e-16, 36.6% identity in 145 aa overlap. Contains Pfam match to entry PF01281 Ribosomal_L9, Ribosomal protein L9, score 203.80, E-value 2.6e-57 and PS00651 Ribosomal protein L9 signature 1 4 9 4 [Reporter]SCO6300 (18I13)_PCR/6226 [Gene]SCO6300/11343 NC_003888 SCIF3.02c, probable secreted hydrolase, len: 615 aa; similar to SW:HEXA_ALTSO (EMBL:D17399) Alteromonas sp. beta-hexosaminidase A precursor (EC 3.2.1.52) Cht60, 598 aa; fasta scores: opt: 565 z-score: 617.5 E(): 7.4e-27; 33.8% identity in 550 aa overlap and to TR:Q9RDG9 (EMBL:AL136519) Streptomyces coelicolor beta-N-acetylglucosaminidase NagA, 615 aa; fasta scores: opt: 2497 z-score: 2289.5 E(): 0; 65.6% identity in 605 aa overlap. Contains Pfam match to entry PF00933 Glyco_hydro_3, Glycosyl hydrolase family 3 N terminal domain and PF01915 Glyco_hydro_3_C, Glycosyl hydrolase family 3 C terminal domain and match to Prosite entry PS00775 Glycosyl hydrolases family 3 active site. Also contains possible N-terminal region signal peptide sequence 1 3 20 8 [Reporter]SCO1912 (15E6)_PCR/4457 [Gene]SCO1912/8123 NC_003888 SCI7.30, probable dihydrodipicolinate synthase, len: 317 aa; similar to many egs. TR:O86841 (EMBL:AL031260) putative dihydrodipicolinate synthase from Streptomyces coelicolor (299 aa) fasta scores; opt: 1018, z-score: 1116.9, E(): 0, (56.4% identity in 305 aa overlap) and SW:DAPA_BRELA dihydrodipicolinate synthase from Brevibacterium lactofermentum (301 aa) fasta scores; opt: 800, z-score: 879.6, E(): 0, (46.6% identity in 292 aa overlap). Contains Pfam match to entry PF00701 DHDPS, Dihydrodipicolinate synthetase family and Prosite matches to PS00665 Dihydrodipicolinate synthetase signature 1 and PS00666 Dihydrodipicolinate synthetase signature 2. 1 4 9 3 [Reporter]SCO4897 (19E13)_PCR/6225 [Gene]SCO4897/11341 NC_003888 2SCK8.23, probable transcriptional regulator, len: 317 aa; similar to TR:Q9KIF0 (EMBL:AF235504) Streptomyces hygroscopicus var. ascomyceticus transcription regulator FkbR1, 297 aa; fasta scores: opt: 726 z-score: 799.4 E(): 0; 45.8% identity in 286 aa overlap. Contains possible helix-turn-helix motif at residues 38..59 (+4.22 SD) 1 3 20 7 [Reporter]SCO3865 (16A6)_PCR/4456 [Gene]SCO3865/8121 NC_003888 SCH18.02, hypothetical protein, len: 260 aa; similar to TR:CAB72374 (EMBL:AL138977) Streptomyces coelicolor hypothetical 27.3 kD protein SC7F9.21c, 247 aa; fasta scores: opt: 296 z-score: 343.9 E(): 1.1e-11; 32.9% identity in 234 aa overlap. Contains and match to Prosite entry 1 3 20 6 [Reporter]SCO3791 (17M2)_PCR/4455 [Gene]SCO3791/8119 NC_003888 SCH63.38, hypothetical protein, len: 445 aa; C-terminal region similar to TR:Q9S2I1 (EMBL:AL109732) Streptomyces coelicolor hypothetical protein SC7H2.36c, 252 aa; fsta scores: opt: 620 Z-score: 451.7 E(): 1.6e-17; 45.175% identity in 228 aa overlap 1 4 8 21 [Reporter]SCO5376 (2I5)_PCR/6221 [Gene]SCO5376/11337 NC_003888 2SC6G5.20c, chiC, chitinase C (EC 3.2.1.14) (putative secreted protein), len: 609aa; previously sequenced as TR:Q9Z9M8 (EMBL:AB017010). Strongly similar to others from Streptomyces spp. e.g. SW:P11220 (CHIT_STRPL) chitinase 63 precursor from Streptomyces plicatus (610 aa) fasta scores; opt: 3997, z-score: 3833.9, E(): 0, 95.1% identity in 611 aa overlap. Contains Pfam match to entry PF00704 Glyco_hydro_18, Glycosyl hydrolases family 18, Pfam match to entry PF00041 fn3, Fibronectin type III domain, Pfam match to entry PF00553 CBD_2, Cellulose binding domain, Prosite match to PS00561 Cellulose-binding domain, bacterial type, Prosite match to PS01095 Chitinases family 18 active site and Prosite match to PS00018 EF-hand calcium-binding domain. Also contains a possible non-cleavable N-terminal signal sequence. 1 3 20 5 [Reporter]SCO5024 (18I2)_PCR/4454 [Gene]SCO5024/8117 NC_003888 SCK15.26, possible oxidoreductase, len: 290 aa; similar to TR:Q9UXP0 (EMBL:AJ011519) Methanolobus tindarius F420-dependent N5,N10-methylene-tetrahydromethanopterin reductase, putative FfdA, 326 aa; fasta scores: opt: 323 z-score: 369.8 E(): 4e-13; 28.3% identity in 240 aa overlap 1 4 8 19 [Reporter]SCO6919 (4A5)_PCR/6219 [Gene]SCO6919/11335 NC_003888 SC1B2.25c, unknown, len: 171 aa. The N-terminus of the predicted protein product of this CDS is hydrophilic. 1 3 20 4 [Reporter]SCP1.272c (19E2)_PCR/4453 [Gene]SCP1.272c/8115 NC_003888 SCP1.272c, unknown, doubtful CDS, len: 148aa; 1 4 8 18 [Reporter]SCO6839 (5M1)_PCR/6218 [Gene]SCO6839/11333 NC_003888 SC3D9.07c, unknown, len: 437 aa. 1 3 20 3 [Reporter]SCO1360 (20A2)_PCR/4452 [Gene]SCO1360/8113 NC_003888 SC10A9.02c, probable gntR-family transcriptional regulator, len: 445 aa; similar to SW:PTSJ_SALTY (EMBL:U11243) Salmonella typhimurium putative transcriptional regulatory protein PtsJ, 430 aa; fasta scores: opt: 968 z-score: 1010.8 E(): 0; 40.4% identity in 433 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and possible helix-turn-helix motif at residues 35..56 (+4.36 SD) 1 4 8 17 [Reporter]SCO5764 (6I1)_PCR/6217 [Gene]SCO5764/11331 NC_003888 SC4H8.03, putative membrane protein, len: 102 aa. Contains possible hydrophobic membrane spanning regions 1 3 20 2 [Reporter]SCO7490 (20I22)_PCR/4451 [Gene]SCO7490/8111 NC_003888 SCBAC17A6.23, possible oxidoreductase, len: 381aa; similar to many eg. TR:Q9F1Z9 (EMBL:AB033991) BtrE from Bacillus circulans (349 aa) fasta scores: opt: 710, Z-score: 676.3, 38.873% identity (41.071% ungapped) in 355 aa overlap. Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases. N-terminus rich in pro/val/ser. 1 3 19 22 [Reporter]SCO1336 (1I18)_PCR/4449 [Gene]SCO1336/8109 NC_003888 2SCG61.18, possible lipoprotein N-acyltransferase (integral membrane), len: 534a; similar to many e.g. SW:P23930 (LNT_ECOLI) apolipoprotein N-acyltransferase (EC 2.3.1.-) from Escherichia coli (512 aa) fasta scores; opt: 391, z-score: 419.0, E(): 7.3e-16, 27.0% identity in 515 aa overlap and TR:O53536 (EMBL:AL021925) hypothetical protein from Mycobacterium tuberculosis (360 aa) fasta scores; opt: 955, z-score: 1020.2, E(): 0, 49.2% identity in 317 aa overlap. C-terminal portion contains Pfam match to entry PF00795 CN_hydrolase, Carbon-nitrogen hydrolase. N-terminal portion contains possible membrane-spanning hydrophobic regions 1 4 8 15 [Reporter]SCO2140 (8A1)_PCR/6215 [Gene]SCO2140/11327 NC_003888 SC6G10.13, possible transcriptional regulator, len: 93 aa; similar to many e.g. TR:O28868 (EMBL:AE001007) transcriptional regulatory protein, AsnC family from Archaeoglobus fulgidus (77 aa) fasta scores; opt: 148, z-score: 216.7, E(): 9.1e-05, (40.8% identity in 71 aa overlap) and SW: LRP_ECOLI Lrp, leucine responsive regulatory protein from Escherichia coli (163 aa) fasta scores; opt: 107, z-score: 157.6, E(): 0.18, (24.7% identity in 85 aa overlap). 1 3 19 21 [Reporter]SCO2504 (2E18)_PCR/4448 [Gene]SCO2504/8107 NC_003888 SCC121.07c, glyS, glycyl-tRNA synthetase, len: 460 aa; similar to SW:SYG_MYCTU (EMBL:Z95208) Mycobacterium tuberculosis glycyl-tRNA synthetase (EC 6.1.1.14) (glycine--tRNA ligase) GlyS, 463 aa; fasta scores: opt: 2210 z-score: 2584.3 E(): 0; 68.3% identity in 457 aa overlap and to SW:SYG_BORBU (EMBL:AE001142) Borrelia burgdorferi glycyl-tRNA synthetase (EC 6.1.1.14) (glycine--tRNA ligase) GlyS, 445 aa; fasta scores: opt: 1090 z-score: 1275.8 E(): 0; 47.3% identity in 457 aa overlap. Contains Pfam match to entry PF00587 tRNA-synt_2b and match to Prosite entry PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1 1 4 8 14 [Reporter]SCO7188 (8M21)_PCR/6214 [Gene]SCO7188/11325 NC_003888 SC8A11.16c, possible secreted peptidase, len: 1210 aa; similar to TR:CAB86111 (EMBL:AL163003) Streptomyces coelicolor putative secreted peptidase SCC24.17c, 1220 aa; fasta scores: opt: 2418 z-score: 2421.7 E(): 0; 41.6% identity in 1257 aa overlap, to SW:SUBT_BACAM (EMBL:K02496) Bacillus amyloliquefaciens subtilisin BPM' precursor (EC 3.4.21.62) Apr, 382 aa; fasta scores: opt: 478 z-score: 483.8 E(): 1.8e-19; 41.4% identity in 290 aa overlap and to Streptomyces coelicolor possible secreted peptidase SC8A11.04c, 1253 aa; fasta scores: opt: 1835 z-score: 1440.0 E(): 0; 46.9% identity in 1279 aa overlap. Contains Pfam matches to entries PF00082 Peptidase_S8, Subtilase family and PF02225 PA, PA domain and matches to Prosite entries PS00136 Serine proteases, subtilase family, aspartic acid active site, PS00137 Serine proteases, subtilase family, histidine active site and PS00138 Serine proteases, subtilase family, serine active site. Also contains possible N-terminal region signal peptide sequence 1 3 19 20 [Reporter]SCO7645 (3A18)_PCR/4447 [Gene]SCO7645/8105 NC_003888 SC10F4.18, possible tetR-family transcriptional regulator, len: 237 aa; similar to TR:Q53901 (EMBL:M64683) Streptomyces coelicolor ORF1-4 (actII) ActII, 259 aa; fasta scores: opt: 213 z-score: 261.0 E(): 5.3e-07; 25.3% identity in 233 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 44..65 (+4.27 SD) 1 4 8 13 [Reporter]SCO6460 (9I21)_PCR/6213 [Gene]SCO6460/11323 NC_003888 SC9B5.27, possible cation-transporting ATPase, len: 638aa; similar to many cation-transporting ATPases eg. SW:COPA_ENTHR copper /potassium-transporting= from Enterococcus hirae (727 aa) fasta scores: opt: 980, z-score: 936.0, E(): 0, (31.9% identity in 630 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site and Pfam match to entry PF00122 E1-E2_ATPase, E1-E2 ATPases. 1 3 19 19 [Reporter]SCO7210 (4M14)_PCR/4446 [Gene]SCO7210/8103 NC_003888 SC2H12.09, hypothetical protein, len: 153 aa. Highly similar to Streptomyces lincolnensis SW:YMEL_STRLN(EMBL:X95703) hypothetical 17.2 kd protein (150 aa), fasta scores opt: 628 z-score: 750.6 E(): 0 56.4% identity in 149 aa overlap 1 4 8 12 [Reporter]SCO2422 (10E21)_PCR/6212 [Gene]SCO2422/11321 NC_003888 SCC42.03c, possible ABC-transporter ATP-binding protein, len: 343 aa; similar to SW:YRBF_HAEIN (EMBL:U32788) Haemophilus influenzae hypothetical ABC-transporter ATP-binding protein HI1087, 264 aa; fasta scores: opt: 680 z-score: 686.6 E(): 8.9e-31; 41.4% identity in 244 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 3 19 18 [Reporter]SCO7672 (5I14)_PCR/4445 [Gene]SCO7672/8101 NC_003888 SC4C2.07, possible integral membrane protein, len: 286 aa. Contains possible hydrophobic membrane spanning regions 1 4 8 5 [Reporter]SCO2967 (17I13)_PCR/6205 [Gene]SCO2967/11307 NC_003888 SCE59.26c, probable carboxy-terminal processing protease precursor, len: 374 aa; similar to SW:CTPA_BARBA (EMBL:L37094) Bartonella bacilliformis carboxy-terminal processing protease precursor (C-terminal processing protease) CtpA, 434 aa; fasta scores: opt: 505 z-score: 527.2 E(): 6e-22; 30.6% identity in 337 aa overlap. Contains Pfam match to entry PF00595 PDZ, PDZ domain (Also known as DHR or GLGF) and possible N-terminal region signal peptide sequence 1 4 8 4 [Reporter]SCO3417 (18E13)_PCR/6204 [Gene]SCO3417/11305 NC_003888 SCE9.24c, probable ABC transporter transmembrane component, len: 315 aa; similar to many e.g. SW:DRRB_STRPE (EMBL:M73758), drrB, Streptomyces peucetius daunorubicin resistance transmembrane protein (283 aa), fasta scores; opt: 507 z-score: 565.3 E(): 3.6e-24, 35.6% identity in 264 aa overlap. Contains hydrophobic, possible membrane-spanning regions 1 4 8 3 [Reporter]SCO4883 (19A13)_PCR/6203 [Gene]SCO4883/11303 NC_003888 2SCK8.09, probable peptidase, len: 410 aa; similar to SW:CBPX_SULSO (EMBL:Z48497) Sulfolobus solfataricus thermostable carboxypeptidase (EC 3.4.17.*) CpsA, 393 aa; fasta scores: opt: 687 z-score: 803.2 E(): 0; 32.0% identity in 372 aa overlap. Contains Pfam match to entry PF01546 Peptidase_M20, Peptidase family M20/M25/M40 1 4 8 2 [Reporter]SCO2964 (20I9)_PCR/6202 [Gene]SCO2964/11301 NC_003888 SCE59.23c, probable lysR-family transcriptional regulator, len: 323 aa; similar to TR:O88132 (EMBL:AJ006517) Streptomyces lipmanii lysR-type transcriptional regulator StgR, 327 aa; fasta scores: opt: 551 z-score: 619.3 E(): 4.4e-27; 36.6% identity in 320 aa overlap and to SW:XAPR_ECOLI (EMBL:X63976) Escherichia coli xanthosine operon regulatory protein XapR, 294 aa; fasta scores: opt: 364 z-score: 412.4 E(): 1.5e-15; 33.6% identity in 301 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Contains possible helix-turn-helix motif at residues 22..43 (+3.25 SD) 1 3 10 2 [Reporter]SCO3051 (20A14)_PCR/4231 [Gene]SCO3051/7699 NC_003888 SCBAC19G2.06c, fadE, acyl-CoA dehydrogenase, len: 385 aa: similar to many e.g. TR:Q9RIQ5 (EMBL:AJ250495) fatty acid acyl CoA dehydrogenase FadE from Streptomyces lividans (385 aa) fasta scores; opt: 2480, Z-score: 2753.5, 99.740% identity (99.740% ungapped) in 385 aa overlap and SW:P45867 (ACDA_BACSU) acyl CoA dehydrogenase from Bacillus subtilis (379 aa) fasta scores; opt: 1214, Z-score: 1351.1, 53.600% identity (54.472% ungapped) in 375 aa overlap. Also similar to TR:Q9RKY7 (EMBL:AL133213) SC6D7.11 Acd acyl CoA dehydrogenase from Streptomyces coelicolor (391 aa) fasta scores; opt: 1079, Z-score: 1201.3, 47.244% identity (48.387% ungapped) in 381 aa overlap. Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase, C-terminal domain; Pfam match to entry PF02770 Acyl-CoA_dh_M, Acyl-CoA dehydrogenase, middle domain; Pfam match to entry PF02771 Acyl-CoA_dh_N, Acyl-CoA dehydrogenase, N-terminal domain; Prosite match to PS00072 Acyl-CoA dehydrogenases signature 1 and Prosite match to PS00073 Acyl-CoA dehydrogenases signature 2. 1 3 9 22 [Reporter]SCO5270 (1A10)_PCR/4229 [Gene]SCO5270/7697 NC_003888 2SC7G11.32, conserved hypothetical protein, len: 340 aa; similar to TR:CAB86114 (EMBL:AL163003) Streptomyces coelicolor hypothetical 37.1 kDa protein SCC24.20, 340 aa; fasta scores: opt: 1113 z-score: 1224.6 E(): 0; 54.5% identity in 343 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 9 20 [Reporter]SCO4729 (3I6)_PCR/4227 [Gene]SCO4729/7695 NC_003888 SC6G4.07, rpoA, DNA-directed RNA polymerase alpha chain, len: 340 aa; almost identical to RPOA_STRCO DNA-directed RNA polymerase alpha chain (EC 2.7.7.6) (339 aa)99.1% id entity in 340 aa overlap, and highly similar to many e.g. R POA_BACSU (314 aa), fasta scores; opt: 917 z-score: 1326.7 E(): 0, 48.9% identity in 311 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 9 19 [Reporter]SCO6043 (4E6)_PCR/4226 [Gene]SCO6043/7693 NC_003888 SC1B5.03c, probable secreted protease, len: 543 aa;similar to e.g. Streptomyces lividans TR:Q54410 (EMBL:L27466) tripeptidylaminopeptidase (537 aa), fasta scores; opt: 1839 z-score: 1633.9 E(): 0, 51.9% identity in 538 aa overlap. Contains possible N-terminal signal sequence 1 3 9 18 [Reporter]SCO5719 (5A6)_PCR/4225 [Gene]SCO5719/7691 NC_003888 SC3C3.05c, unknown, len: 266 aa 1 3 9 17 [Reporter]SCO6655 (6M2)_PCR/4224 [Gene]SCO6655/7689 NC_003888 SC5A7.05, ribA2, GTP cyclohydrolase II, len: 221 aa; highly similar to the C-terminus of many RibA genes where the GTP cyclohydrolase activity is, e.g. GCH2_BACSU GTP cyclohydrolase II (EC 3.5.4.25) (398 aa), fasta sores; opt: 656 z-score: 960.9 E(): 0, 53.1% identity in 196 aa overlap and to SW:GCH2_ARATH (EMBL:D45165) Arabidopsis thaliana GTP cyclohydrolase II (EC 3.5.4.25) 245 aa; fasta scores: opt: 656 Z-score: 760.1 E(): 1.1e-34; 51.832% identity in 191 aa overlap 1 3 9 16 [Reporter]SCO3602 (7I2)_PCR/4223 [Gene]SCO3602/7687 NC_003888 SC66T3.13c, probable transmembrane transport protein, len: 403 aa; similar to many putative ion transport proteins and to part of SW:KEFC_ECOLI (EMBL:X56742), kefC, Escherichia coli glutathione-regulated potassium-efflux system protein (620 aa), fasta scores; opt: 328 z-score: 319.2 E(): 1.9e-10, 27.1% identity in 410 aa overlap. Similar to TR:O69958 (EMBL:AL022268) S.coelicolor putative transmembrane transport protein (411 aa) (74.9% identity in 399 aa overlap). Contains hydrophobic, possible membrane-spanning regions 1 3 9 15 [Reporter]SCO2152 (8E2)_PCR/4222 [Gene]SCO2152/7685 NC_003888 SC6G10.25, possible response regulator, len: 133aa; similar to TR:P95183 (EMBL:Z83867) hypothetical protein from Mycobacterium tuberculosis (133 aa) fasta scores; opt: 474, z-score: 602.8, E(): 2.8e-26, (54.2% identity in 120 aa overlap) and TR:E1358527 (EMBL:AJ131213) response regulator from Streptomyces coelicolor (234 aa) fasta scores; opt: 122, z-score: 162.8, E(): 0.091, (28.5% identity in 130 aa overlap) 1 3 9 14 [Reporter]SCO6603 (9A2)_PCR/4221 [Gene]SCO6603/7683 NC_003888 SC8A6.24, probable transmembrane sugar transport pro tein, len: 279 aa; similar to e.g. S. coelicolor TR:P72399 (EMBL:Y07706) putative maltose permease malG (302 aa), fast a scores; opt: 542 z-score: 755.1 E(): 0, 36.7% identity in 270 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature and Pfam m atch to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 75.20, E -value 1.3e-18 1 3 9 12 [Reporter]SCO2760 (10I22)_PCR/4219 [Gene]SCO2760/7681 NC_003888 SCC57A.31, possible membrane protein, len: 216 aa. Contains a possible membrane spanning hydrophobic domain. 1 3 9 11 [Reporter]SCO4088 (11E22)_PCR/4218 [Gene]SCO4088/7679 NC_003888 SCD25.24c, unknown, len: 84. Highly similar in N-terminus to Mycobacterium tuberculosis TR: O53824 (EMBL; AL022004) hypothetical 6.9 KD protein (60 aa), fasta scores opt: 180 z-score: 231.6 E(): 1.5e-05 51.8% identity in 56 aa overlap. 4 3 21 4 [Reporter]SCO6104 (19L2)_PCR/5986 [Gene]SCO6104/10897 NC_003888 SCBAC1A6.28c, possible secreted protein, len: 437 aa. Contains possible N-terminal region signal peptide sequence 1 3 9 10 [Reporter]SCO2118 (12A22)_PCR/4217 [Gene]SCO2118/7677 NC_003888 SCE10.12c, dldh2, probable D-lactate dehydrogenase, len: 331 aa; highly similar to many members of the D-isomer specific 2-hydroxyacid dehydrogenases family, especially lactate dehydrogenases e.g. SW:LDHD_ECOLI (EMBL:U36928), LdhA, Escherichia coli D-lactate dehydrogenase (329 aa), fasta scores; opt: 843 z-score: 1010.7 E(): 0, 44.4% identity in 320 aa overlap. Similar to other dehydrogenases from S.coelicolor e.g. SC66T3.05 (EMBL:AL079348), dldh, S.coelicolor probable D-lactate dehydrogenase 337 aa) (36.3% identity in 325 aa overlap). Contains Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases 4 3 21 2 [Reporter]SCO7708 (20P22)_PCR/5984 [Gene]SCO7708/10895 NC_003888 SCBAC12C8.09c, conserved hypothetical protein, len: 216aa: similar to many eg. TR:O86609 (EMBL:AL031155) hypothetical protein from Streptomyces coelicolor (211 aa) fasta scores; opt: 764, Z-score: 865.8, 54.673% identity (55.981% ungapped) in 214 aa overlap. 1 3 9 9 [Reporter]SCO0134 (13M18)_PCR/4216 [Gene]SCO0134/7675 NC_003888 SCJ21.15c, unknown, len: 101 aa 4 3 20 22 [Reporter]SCO1344 (1P18)_PCR/5982 [Gene]SCO1344/10893 NC_003888 2SCG61.26c, possible solute binding transport lipoprotein, len: 530aa; similar to many eg. TR:Q9RJ53 (EMBL:AL132648) putative lipoprotein oligopeptide binding protein (534 aa) fasta scores; opt: 1438, z-score: 1620.4, E(): 0, 43.4% identity in 539 aa overlap and SW:P76128 (YDDS_ECOLI) putative binding protein YddS precursor (516 aa) fasta scores; opt: 532, z-score: 600.2, E(): 5.9e-26, 25.7% identity in 517 aa overlap. Contains two Pfam match to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5 and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 1 3 9 8 [Reporter]SCO1522 (14I18)_PCR/4215 [Gene]SCO1522/7673 NC_003888 SCL2.12c, hypothetical protein, len: 202 aa; similar to TR:Q49637 (EMBL:U00011) Mycobacterium leprae HisH protein, 219 aa; fasta scores: opt: 715 z-score: 849.9 E(): 0; 58.9% identity in 192 aa overlap and to TR:AAF10937 (EMBL:AE001982) Deinococcus radiodurans amidotransferase HisH, putative, 196 aa; fasta scores: opt: 508 z-score: 607.8 E(): 1.8e-26; 47.3% identity in 186 aa overlap. Contains Pfam match to entry PF01174 UPF0030, Uncharacterized protein family UPF0030 4 3 20 21 [Reporter]SCO5791 (2L18)_PCR/5981 [Gene]SCO5791/10891 NC_003888 SC4H2.12, miaA, probable tRNA delta(2)-isopentenylpyrophosphate transferase, len: 312 aa; similar to many e.g. SW:MIAA_ECOLI (EMBL:P16384) Escherichia coli tRNA delta(2)-isopentenylpyrophosphate transferase (EC 2.5.1.8) (316 aa), fasta scores; opt: 627 z-score: 781.4 E(): 0, 39.9% identity in 286 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 9 7 [Reporter]SCO5488 (15E18)_PCR/4214 [Gene]SCO5488/7671 NC_003888 SC2A11.22, trmU, probable tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase, len: 376 aa; similar to many e.g. TRMU_BACSU probable tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase (370 aa), fasta scores; opt: 708 z-score: 748.3 E(): 0, 38.1% identity in 365 aa overlap 1 3 9 6 [Reporter]SCO3885 (16A18)_PCR/4213 [Gene]SCO3885/7669 NC_003888 StH24.07, conserved hypothetical GidB-family protein, len: 239 aa; previously sequenced as TR:O54571 (EMBL:Y16311) Streptomyces coelicolor gidB-like protein (239 aa). Similar to many members of the gidB family of hypothetical proteins e.g. TR:O53597 (EMBL:AL021426) Mycobacterium tuberculosis hypothetical protein (224 aa), fasta scores; opt: 654 z-score: 756.9 E(): 0, 48.0% identity in 221 aa overlap 4 3 20 19 [Reporter]SCO5606 (4D18)_PCR/5979 [Gene]SCO5606/10887 NC_003888 SC2E1.23c, unknown prophage gene, len: 96 aa 1 3 9 5 [Reporter]SCO3231 (17M14)_PCR/4212 [Gene]SCO3231/7667 NC_003888 SCE63.02c, cdaPS2, CDA peptide synthetase II, len: 3670 aa; part of the calcium-dependent antibiotic (CDA) biosynthetic cluster from Streptomyces coelicolor. CDA is a peptide antibiotic which is synthesised non-ribosomally by a putative multifunctional peptide synthetase enzyme. This CDS encodes a subunit of this enzyme and is suspected to be responsible for the addition of 3 amino acids to the peptide antibiotic. This ORF overlaps the upstream (cdaPSI) and downstream (cdaPSIII) ORFs by one base indicating possible translational coupling of all three. Contains four separate Pfam matches to entry PF00668 DUF4, Domain of unknown function (U), three separate Pfam matches to entry PF00501 AMP-binding, AMP-binding enzyme (A) and three separate Pfam matches to entry PF00550 pp-binding, Phosphopantetheine attachment site (P). These Pfam matches cover the full length of the protein in the following order from N to C-terminal U-A-P-U-A-P-U-A-P-U. The first and third Pfam matches to entry PF00501 also contain Prosite matches to PS00455 Putative AMP-binding domain signature. All three Pfam matches to entry PF00550 also contain Prosite matches to PS00012 Phosphopantetheine attachment site. 4 3 20 18 [Reporter]SCO7679 (5P14)_PCR/5978 [Gene]SCO7679/10885 NC_003888 SC4C2.14, possible transport system integral membrane protein, len: 284 aa; similar to SW:Y4TQ_RHISN (EMBL:Z68203) Rhizobium sp. probable peptide ABC transporter permease protein Y4tQ, 291 aa; fasta scores: opt: 433 z-score: 466.4 E(): 1.9e-18; 33.2% identity in 277 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 9 4 [Reporter]SCP1.300 (18I14)_PCR/4211 [Gene]SCP1.300/7665 NC_003888 SCP1.300, possible acetaldehyde dehydrogenase (acylating), len: 293aa; similar to many eg. TR:Q51962 (EMBL:U13232) acetaldehyde dehydrogenase (acylating) from Pseudomonas putida (307 aa) fasta scores; opt: 550, z-score: 658.2, E(): 3.4e-29, 35.0% identity in 294 aa overlap. 4 3 20 17 [Reporter]SCO6749 (6L14)_PCR/5977 [Gene]SCO6749/10883 NC_003888 SC5F2A.32, unknown, len: 161 aa; similar to other hypothetical proteins egs. TR:O54111 (EMBL:AL021529) from Streptomyces coelicolor (171 aa) fasta scores; opt: 221, z-score: 281.8, E(): 2.2e-08, (35.8% identity in 137 aa overlap) and TR:O69204 (EMBL:U33059) Actinosynnema pretiosum auranticum (144 aa) fasta scores; opt: 220, z-score: 281.8, E(): 2.2e-08, (38.7% identity in 155 aa overlap). 1 3 9 3 [Reporter]SCO4036 (19E14)_PCR/4210 [Gene]SCO4036/7663 NC_003888 2SCD60.02c, hypothetical protein, len: 97 aa; identical to previously sequenced SW:YRPX_STRCO (EMBL:L11648) Streptomyces coelicolor hypothetical 11.1 kDa protein in RpoX 5' region, 97 aa 4 3 20 15 [Reporter]SCO5219 (8D14)_PCR/5975 [Gene]SCO5219/10881 NC_003888 SC7E4.16, possible lipoprotein, len: 418 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site, possible N-terminal region signal peptide sequence and possible hydrophobic membrane spanning regions 1 3 9 2 [Reporter]SCO3362 (20M10)_PCR/4209 [Gene]SCO3362/7661 NC_003888 SCE94.13, putative membrane protein, len: 211 aa; weakly similar to many eg. SW:YRHP_BACSU hypothetical protein located close to sigma factor genes in Bacillus subtilis (210 aa) fasta scores; opt: 192, z-score: 222.0, E(): 4.7e-05, (25.0% identity in 164 aa overlap). Contains a possible membrane spanning hydrophobic regions 1 3 8 22 [Reporter]SCO4217 (1M6)_PCR/4207 [Gene]SCO4217/7659 NC_003888 2SCD46.31c, conserved hypothetical protein, len: 294 aa; similar to TR:P75863 (EMBL:AE000196) hypothetical protein from Escherichia coli (369 aa) fasta scores; opt: 244, z-score: 297.9, E(): 4e-09, 33.3% identity in 288 aa overlap. 4 3 20 12 [Reporter]SCO2497 (11H10)_PCR/5972 [Gene]SCO2497/10877 NC_003888 SCC82.03c, hypothetical protein, len: 406 aa; similar to varous hypothetical proteins, e.g. SW:Y823_MYCTU (EMBL:AL022004) Mycobacterium tuberculosis hypothetical 41.4 kD protein RV0823c, 389 aa; fasta scores: opt: 1153 z-score: 1349.3 E(): 0; 56.2% identity in 406 aa overlap. Contains Pfam match to entry PF01207 UPF0034, Uncharacterized protein family UPF0034 and match to Prosite entry PS01136 Uncharacterized protein family UPF0034 signature 1 3 8 21 [Reporter]SCO2618 (2I6)_PCR/4206 [Gene]SCO2618/7657 NC_003888 SCC80.03c, clpP2, ATP dependent Clp protease proteolytic subunit 2, len: 236 aa; identical to previously sequenced TR:Q9ZH58 (EMBL:AF071885) Streptomyces coelicolor ATP dependent Clp protease proteolytic subunit 2 (EC 3.4.21.92) ClpP2, 236 aa. Contains Pfam match to entry PF00574 CLP_protease, Clp protease and match to Prosite entry PS00381 Endopeptidase Clp serine active site 4 3 20 10 [Reporter]SCO3276 (13P6)_PCR/5970 [Gene]SCO3276/10875 NC_003888 SCE39.26, possible integral membrane transporter, len: 499aa; similar to many eg. TR:Q50443 (EMBL:U50335) ORF2 from Mycobacterium smegmatis (498 aa) fasta scores; opt: 2027, z-score: 2225.4, E(): 0, (64.0% identity in 478 aa overlap) and TR:O07488 (EMBL:Y13308) proposed sulphate permease from Yersinia enterocolitica (492 aa) fasta scores; opt: 1564, z-score: 1718.0, E(): 0, (51.0% identity in 482 aa overlap). Contains Pfam match to entry PF00916 Sulfate_transp, Sulfate transporter family. Also contains several possible membrane spanning hydrophobic regions. 1 3 8 20 [Reporter]SCO4777 (3E6)_PCR/4205 [Gene]SCO4777/7655 NC_003888 SCD63.09, pkaD, protein serine/threonine kinase, len: 599 aa; identical to previously sequenced TR:O83032 (EMBL:AB016932) Streptomyces coelicolor protein serine/threonine kinase PkaD. Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and matches to Prosite entries PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature and possible hydrophobic membrane spanning region 4 3 20 9 [Reporter]SCO0695 (14L6)_PCR/5969 [Gene]SCO0695/10873 NC_003888 SCF42.05, unknown, len: 328 aa; similar to TR:Q9KZT0 (EMBL:AL353832) hypothetical protein SCE6.08 from Streptomyces coelicolor (326 aa) fasta scores; opt: 371, Z-score: 407.1, E(): 4.8e-15, 35.629% identity (39.145% ungapped) in 334 aa overlap. 1 3 8 19 [Reporter]SCO6927 (4A6)_PCR/4204 [Gene]SCO6927/7653 NC_003888 SC1B2.33c, unknown, len: 374 aa. 4 3 20 8 [Reporter]SCO4839 (15H6)_PCR/5968 [Gene]SCO4839/10871 NC_003888 SC5G8.07, trpS2, tryptophanyl-tRNA synthetase, len: 339aa; strongly similar to many eg. SW:P00953 (SYW_BACST) tryptophanyl-tRNA synthetase from Bacillus stearothermophilus (328 aa) fasta scores; opt: 1195, z-score: 1347.2, E(): 0, 55.7% identity in 325 aa overlap. Also similar to TR:Q9X8F8 (EMBL:AL049819) trpS, tryptophanyl-tRNA synthetase (fragment) from Streptomyces coelicolor (208 aa) fasta scores; opt: 618, z-score: 703.4, E(): 1e-31, 50.5% identity in 204 aa overlap.Contains Pfam match to entry PF00579 tRNA-synt_1b, tRNA synthetases class I (W and Y) and Prosite match to PS00178 Aminoacyl-transfer RNA synthetases class-I signature. 1 3 8 18 [Reporter]SCO6847 (5M2)_PCR/4203 [Gene]SCO6847/7651 NC_003888 SC3D9.15c, unknown, len: 206 aa. Contains a 24 amino acid direct repeat (ATYRTVSRRLSRRLASFSARAEDR) between residues 129..152 and 154..177. 4 1 14 5 [Reporter]SCO3338 (17D24)_PCR/1802 [Gene]SCO3338/3299 NC_003888 SCE7.05c, probable integral membrane protein, len: 266aa; similar to many hypothetical proteins eg. TR:O33188 (EMBL:Z98268) from Mycobacterium tuberculosis (226 aa) fasta scores; opt: 661, z-score: 725.6, E(): 4.3e-33, (45.1% identity in 226 aa overlap). Contains possible membrane spanning hydrophobic regions. 1 3 8 17 [Reporter]SCO5778 (6I2)_PCR/4202 [Gene]SCO5778/7649 NC_003888 SC4H8.17, two-component system transcriptional regulator, len: 228 aa; similar to many eg. VANR_ENTFA Q47744 regulatory protein VanR from enterococcuus faecalis (220 aa), fasta scores; opt: 469 z-score: 742.2 E(): 0, 37.1% identity in 224 aa overlap 4 3 20 6 [Reporter]SCO1916 (17P2)_PCR/5966 [Gene]SCO1916/10867 NC_003888 SCI7.34c, possible transferase, len: 329 aa; similar to many e.g. TR:BAA75911 (EMBL:AB024601) tetrahydrodipicolinate N-succinyltransferase from Pseudomonas aeruginosa (344 aa) fasta scores; opt: 1004, z-score: 1062.7, E(): 0, (54.4% identity in 307 aa overlap). Contains possible N-terminal signal sequence. 1 3 8 16 [Reporter]SCO0305 (7E2)_PCR/4201 [Gene]SCO0305/7647 NC_003888 SC5G9.14, hypothetical protein, len: 959 aa; unknown function, similar to TR:O54182 (EMBL:AL021411) Streptomyces coelicolor hypothetical protein (913 aa), fasta scores; opt: 698 z-score: 776.6 E(): 0, 27.0% identity in 972 aa overlap. The C-terminus shows very weak similarity to eukaryotic beta-transducins e.g. SW:GBB4_MOUSE (EMBL:S86124), gnb4, Mus musculus guanine nucleotide-binding protein beta subunit 4 (340 aa) (23.0% identity in 291 aa overlap). Contains possible hydrophobic membrane spanning region 4 3 20 5 [Reporter]SCO0385 (18L2)_PCR/5965 [Gene]SCO0385/10865 NC_003888 SCF62.11, possible membrane protein, len: 521 aa. Contains possible membrane spanning hydrophobic domains and two possible coiled-coils from 144 to 182 (39 residues) and from 190 to 221 (32 residues). 1 3 8 15 [Reporter]SCO2148 (8A2)_PCR/4200 [Gene]SCO2148/7645 NC_003888 SC6G10.21c, qcrB, cytochrome B subunit, len: 545 aa; almost identical to one previously sequenced in Streptomyces lividans TR:G4154324 (EMBL:AF107888) (549 aa) fasta scores: opt: 3501, z-score: 3791.6, E(): 0, (95.5% identity in 550 aa overlap). Also similar to SW:QCRB_BACST cytochrome B subunit from Bacillus stearothermophilus (224 aa) fasta scores; opt: 409, z-score: 451.8, E(): 7.2e-18, (30.0% identity in 240 aa overlap). Contains Pfam match to entry PF00033 cytochrome_b_N, Cytochrome b(N-terminal)/b6/petB. Also contains several probable membrane spanning hydrophobic regions. 4 3 20 4 [Reporter]SCP1.236 (19H2)_PCR/5964 [Gene]SCP1.236/10863 NC_003888 SCP1.236, mmyK, possible ATP/GTP-binding protein, len: 206aa; previously sequenced and annotated as TR:Q9JN85 (EMBL:AJ276673). Also similar to neighbouring CDS SCP1.233A MmyX, putative ATP/GTP-binding protein (234 aa) (previously sequenced as TR:Q9JN81 (EMBL:AJ276673) hypothetical protein) fasta scores; opt: 538, z-score: 647.7, E(): 1.3e-28, 42.7% identity in 199 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 8 14 [Reporter]SCO7196 (8M22)_PCR/4199 [Gene]SCO7196/7643 NC_003888 SC8A11.24c, possible ion transport integral membrane protein, len: 288 aa; similar to SW:KCSA_STRLI (EMBL:Z37969) Streptomyces lividans voltage-gated potassium channel KcsA, 160 aa; fasta scores: opt: 302 z-score: 373.1 E(): 2.6e-13; 41.0% identity in 156 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 20 3 [Reporter]SCP1.149c (20D2)_PCR/5963 [Gene]SCP1.149c/10861 NC_003888 SCP1.149c, unknown, len: 369aa; similar to SCP1.181c (pseudogene) fasta scores; opt: 331, z-score: 405.0, E(): 4.9e-18, 44.2% identity in 120 aa overlap. 1 3 8 13 [Reporter]SCO1485 (9I22)_PCR/4198 [Gene]SCO1485/7641 NC_003888 SC9C5.09c, possible integral membrane protein, len: 191 aa; similar to TR:P71810 (EMBL:Z81011) Mycobacterium tuberculosis hypothetical 18.2 kD protein, 165 aa; fasta scores: opt: 301 z-score: 362.9 E(): 9.7e-13; 33.1% identity in 148 aa overlap. Contains possible hydrophobic membrane spanning region 4 1 13 21 [Reporter]SCO5141 (2P12)_PCR/1796 [Gene]SCO5141/3289 NC_003888 SCP8.04c, folP, dihydropteroate synthase, len: 294 aa; similar to SW:DHPS_MYCLE (EMBL:U15180) Mycobacterium leprae dihydropteroate synthase (EC 2.5.1.15) (DHPS) FolP, 291 aa; fasta scores: opt: 1095 z-score: 1239.8 E(): 0; 60.6% identity in 292 aa overlap. Contains Pfam match to entry PF00809 DHPS, Dihydropteroate synthase and matches to Prosite entries PS00792 Dihydropteroate synthase signature 1 and PS00793 Dihydropteroate synthase signature 2 1 3 8 12 [Reporter]SCO2430 (10E22)_PCR/4197 [Gene]SCO2430/7639 NC_003888 SCC24.01, possible sugar hydrolase (fragment) (putative secreted protein), len: >407 aa; similar to C-terminal region of SW:BGAL_ASPNG (EMBL:L06037) Aspergillus niger beta galactosidase precursor (EC 3.2.1.23) LacA, 1006 aa; fasta scores: opt: 214 z-score: 223.7 E(): 5e-05; 27.1% identity in 321 aa overlap,SCC42.11, probable secreted beta-galactosidase (fragment), len: >555 aa; similar to SW:BGAL_ASPNG (EMBL:L06037) Aspergillus niger beta-galactosidase precursor (EC 3.2.1.23) LacA, 1006 aa; fasta scores: opt: 413 z-score: 403.4 E(): 5.4e-15; 24.3% identity in 608 aa overlap. Contains possible N-terminal region signal peptide sequence 4 3 19 22 [Reporter]SCO1339 (1L18)_PCR/5960 [Gene]SCO1339/10857 NC_003888 2SCG61.21, hypothetical protein, len: 206 aa; similar to TR:Q9X9V5 (EMBL:AL096743) hypothetical protein from Streptomyces coelicolor (197 aa) fasta scores; opt: 212, z-score: 254.4, E(): 1.1e-06, 33.9% identity in 186 aa overlap. 1 3 8 11 [Reporter]SCO4082 (11A22)_PCR/4196 [Gene]SCO4082/7637 NC_003888 SCD25.18, possible secreted peptidase, len: 279 aa. Shares a very low level of similarity, in parts, with two other putative Streptomyces coelicolor peptidases: TR:Q9ZBY3 (EMBL; AL034355) SCD78.16 (349 aa), fasta scores opt: 199 z-score: 225.8 E(): 3.2e-05 44.3% identity in 79 aa overlap and TR:CAB40868 (EMBL; AL049628) SCE94.19C (228 aa), fasta scores opt: 191 z-score: 219.8 E(): 6.8e-05 41.7% identity in 72 aa overlap. Contains a Pfam match to entry PF01551 Peptidase_M37 and a possible N-terminal signal sequence. 4 3 19 21 [Reporter]SCO4296 (2H18)_PCR/5959 [Gene]SCO4296/10855 NC_003888 SCD95A.29, groEL2, 60 kD chaperonin 2, len: 541 aa; identical to SW:CH62_STRAL (EMBL:M76658) Streptomyces albus g. 60 kD chaperonin 2 GroEL2, 539 aa and highly similar to TR:CAA20418 (EMBL:AL031317) Streptomyces coelicolor 60 kD chaperonin cpn60, groEL1, 541 aa; fasta scores: opt: 2371 z-score: 2327.6 E(): 0; 69.6% identity in 40 aa overlap. Contains Pfam match to entry PF00118 cpn60_TCP1, TCP-1/cpn60 chaperonin family and match to Prosite entry PS00296 Chaperonins cpn60 signature 1 3 8 10 [Reporter]SCO2920 (12M18)_PCR/4195 [Gene]SCO2920/7635 NC_003888 SCE19A.20c, probable secreted protease, len: 781 aa; similar to SW:INA_BACTL (EMBL:X55436), Ina, Bacillus thuringiensis inhibitor A (a protease) (687 aa), fasta scores; opt: 924 z-score: 991.3 E(): 0, 32.9% identity in 747 aa overlap and TR:O07351 (EMBL:Y00557), PrtV, Vibrio cholerae protease precursor (919 aa) (33.2% identity in 794 aa overlap). Contains probable N-terminal signal sequence and PS00142 Neutral zinc metallopeptidases, zinc-binding region signature 4 3 19 20 [Reporter]SCO7648 (3D18)_PCR/5958 [Gene]SCO7648/10853 NC_003888 SC10F4.21c, probable two-component system response regulator, len: 221 aa; similar to many other Streptomyces coelicolor response regulators, e.g. TR:Q9RKT8 (EMBL:AL132824) putative response regulator SCAH10.19, 229 aa; fasta scores: opt: 713 z-score: 802.2 E(): 0; 57.2% identity in 208 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Also contains possible helix-turn-helix motif at residues 163..184 (+3.19 SD) 1 3 8 9 [Reporter]SCO0620 (13I18)_PCR/4194 [Gene]SCO0620/7633 NC_003888 SCF56.04c, hypothetical protein, len: 92 aa; similar to various hypothetical proteins, e.g. TR:BAA86632 (EMBL:AB033763) Staphylococcus aureus hypothetical 9.9 kD protein, 88 aa; fasta scores: opt: 203 z-score: 280.2 E(): 3.2e-08; 43.7% identity in 71 aa overlap 4 3 19 19 [Reporter]SCO7213 (4P14)_PCR/5957 [Gene]SCO7213/10851 NC_003888 SC2H12.12, unknown, len: 248 aa. Similar to Streptomyces coelicolor TR:CAB89463(EMBL:AL354048) putative fatty acid synthase, SCE25.32C (438 aa), fasta scores opt: 195 z-score: 237.4 E(): 9.5e-06 26.3% identity in 232 aa overlap. Also highly similar to Escherichia coli SW:YJHP_ECOLI(EMBL:U14003) hypothetical 27.4 kd protein (248 aa), fasta scores opt: 1174 z-score: 1413.2 E():0 66.5% identity in 248 aa overlap. 1 3 8 8 [Reporter]SCO3826 (14E18)_PCR/4193 [Gene]SCO3826/7631 NC_003888 SCGD3.27c, possible membrane protein, len: 365 aa; unknown function, weakly similar to C-terminal half of SW:KEFC_ECOLI (EMBL:X56742), KefC, Escherichia coli glutathione-regulated potassium-efflux system protein (620 aa), fasta scores; opt: 164 z-score: 190.7 E(): 0.0027, 21.4% identity in 308 aa overlap. Highest similarity to TR:O53346 (EMBL:AL021646) Mycobacterium tuberculosis hypothetical protein (355 aa) (49.8% identity in 325 aa overlap). Also weakly similar to C-terminal half of SC66T3.20, S.coelicolor possible membrane protein (623 aa) (24.6% identity in 329 aa overlap) and shares regions of local similarity with other putative S.coelicolor membrane proteins. Contains hydrophobic, possible membrane-spanning regions 4 1 13 16 [Reporter]SCO6769 (7L8)_PCR/1791 [Gene]SCO6769/3279 NC_003888 SC6A5.18, probable aminotransferase, len: 461 aa; similar to many e.g. SW:ARGD_BACSU acetylornithine aminotransferase from Bacillus subtilis (385 aa) fasta scores; opt: 663, z-score: 757.6, E(): 0, (37.8% identity in 341 aa overlap). Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate and Prosite match to PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. 1 3 8 7 [Reporter]SCO1078 (15A18)_PCR/4192 [Gene]SCO1078/7629 NC_003888 SCG22.24, probable lacI-family transcriptional regulator, len: 349 aa; similar to TR:Q9S1T0 (EMBL:AL109950) Streptomyces coelicolor putative lacI-family transcriptional regulator SCJ4.43c, 367 aa; fasta scores: opt: 786 z-score: 894.0 E(): 0; 38.5% identity in 353 aa overlap and to SW:GALR_ECOLI (EMBL:V00280) Escherichia coli galactose operon repressor GalR, 343 aa; fasta scores: opt: 504 z-score: 575.9 E(): 1.3e-24; 31.3% identity in 336 aa overlap. Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and match to Prosite entry PS00356 Bacterial regulatory proteins, lacI family signature. Contains also a possible helix-turn-helix motif at residues 29..50 (+4.16 SD) 4 3 19 15 [Reporter]SCO6012 (8P10)_PCR/5954 [Gene]SCO6012/10847 NC_003888 SC7B7.09c, probable hydrolase (putative secreted protein), len: 507 aa; similar to many chitinases eg. TR:Q59145 (EMBL:D31818) chitinase II precursor (EC 3.2.1.14) Aeromonas sp. (542 aa), fasta scores; opt: 410 z-score: 512.7 E(): 2.1e-21, 32.1% identity in 498 aa overlap. Contains N-terminal signal sequence and PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 8 6 [Reporter]SCO3152 (16M14)_PCR/4191 [Gene]SCO3152/7627 NC_003888 SCE87.03c, unknown, len: 145 aa. 4 3 19 14 [Reporter]SCO7003 (9L10)_PCR/5953 [Gene]SCO7003/10845 NC_003888 SC8F11.29c, unknown, len: 336 aa. Similar in parts to a protein of undefined function from Streptomyces coelicolor: TR:CAB46782(EMBL:AL096811) SCI30A.05C (338 aa), fasta scores opt: 249 z-score: 278.0 E(): 4.7e-08 34.8% identity in 319 aa overlap. 1 3 8 5 [Reporter]SCO3541 (17I14)_PCR/4190 [Gene]SCO3541/7625 NC_003888 SCH5.03c, possible DNA polymerase, len: 401 aa; limited region of similarity to many DNA polymerase III subunits e.g. SW:DP3X_SALTY DNA polymerase III subunits gamma and tau from Salmonella typhimurium (642 aa) fasta scores; opt: 327, z-score: 338.5, E(): 1.5e-11, (32.1% identity in 246 aa overlap). Contains probable coiled-coil region from 245 to 285 (41 residues), max score: 1.601, (probability 0.99) 4 3 19 13 [Reporter]SCO3793 (10H10)_PCR/5952 [Gene]SCO3793/10843 NC_003888 SCAC2.01, conserved hypothetical protein, len: 121 aa; similar to TR:O34593 (EMBL:Z99110) Bacillus subtilis YjqA protein, 125 aa; fasta scores: opt: 459 z-score: 636.3 E(): 6.2e-28; 60.5% identity in 114 aa overlap 1 3 8 4 [Reporter]SCO1589 (18E14)_PCR/4189 [Gene]SCO1589/7623 NC_003888 SCI35.11, hypothetical protein, len: 64 aa; similar to two small hypothetical proteins from M. tuberculosis e.g. YU07_MYCTU MTCY339.07C (80 aa), fasta scores; opt: 169 z-score: 320.8 E(): 1.3e-10, 46.6% identity in 58 aa overlap 4 3 19 12 [Reporter]SCO2623 (11D10)_PCR/5951 [Gene]SCO2623/10841 NC_003888 SCC80.08c, hypothetical protein, len: 164 aa; similar to N-terminal domain of SW:RELA_CORGL (EMBL:AF038651) Corynebacterium glutamicum GTP pyrophosphokinase (EC 2.7.6.5) RelA, 760 aa; fasta scores: opt: 162 z-score: 170.8 E(): 0.054; 33.6% identity in 149 aa overlap. Contains 3x conserved repeat: MPP 1 3 8 3 [Reporter]SCP1.223c (19A14)_PCR/4188 [Gene]SCP1.223c/7621 NC_003888 SCP1.223c, unknown, len: 117aa; 4 1 13 11 [Reporter]SCO4791 (12H4)_PCR/1786 [Gene]SCO4791/3269 NC_003888 SCD63A.02, possible two-component system sensor kinase, len: 430 aa; C-terminal domain similar to TR:CAB51969 (EMBL:AL109661) Streptomyces coelicolor putative two-component system sensor kinase SC6E10.15c, 413 aa; fasta scores: opt: 168 z-score: 198.3 E(): 0.0014; 32.7% identity in 248 aa overlap. Contains possible hydrophobic membrane regions in N-terminal domain 1 3 8 2 [Reporter]SCO3572 (20I10)_PCR/4187 [Gene]SCO3572/7619 NC_003888 SCH17.06c, possible hydrolase, len: 256 aa; weakly similar to eukaryotic glyoxalase e.g. SW:GL2M_ARATH (EMBL:U90928), glx2-1, Arabidopsis thaliana hydroxyacylglutathione hydrolase (331 aa), fasta scores; opt: 186 z-score: 218.1 E(): 8e-05, 29.2% identity in 161 aa overlap. Similar to many hypothetical proteins e.g. TR:O69645 (EMBL:AL022121) Mycobacterium tuberculosis putative hydrolase (264 aa) (54.7% identity in 247 aa overlap) 4 3 19 10 [Reporter]SCO3272 (13L6)_PCR/5949 [Gene]SCO3272/10837 NC_003888 SCE39.22c, possible DNA-binding protein, len: 122aa; some similarity to TR:O64356 (EMBL:AF064539) Gp48 protein from bacteriophage N15 (95 aa) fasta scores; opt: 114, z-score: 155.2, E(): 0.25, (36.6% identity in 71 aa overlap). Contains probable helix-turn-helix (+6.85 SD) 82-103aa. 1 3 7 22 [Reporter]SCO4213 (1I6)_PCR/4185 [Gene]SCO4213/7617 NC_003888 2SCD46.27, unknown, len: 544 aa; C-terminal region similar to that of SW:P24202 (MRR_ECOLI) Mrr restriction system protein from Escherichia coli (304 aa) fasta scores; opt: 199, z-score: 216.8, E(): 0.00013, 28.4% identity in 162 aa overlap. N-terminal region contains possible coiled-coil sequences residues (33-63 and 156-200) and two TTA encoded leucine residues which may be a target for bldA regulation. 4 3 19 9 [Reporter]SCO0954 (14H6)_PCR/5948 [Gene]SCO0954/10835 NC_003888 SCM11.09c, possible acetyltransferase, len: 160 aa; similar to various hypothetical proteins, e.g. TR:Q51670 (EMBL:U34346) Paracoccus denitrificans hypothetical 18.8 kD protein, 173 aa; fasta scores: opt: 427 z-score: 515.7 E(): 2.4e-21; 45.3% identity in 161 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 3 7 21 [Reporter]SCO2431 (2E6)_PCR/4184 [Gene]SCO2431/7615 NC_003888 SCC24.02c, abfA, alpha-L-arabinofuranosidase, len: 501 aa; almost identical to SW:ABFA_STRLI (EMBL:U04630) Streptomyces lividans alpha-L-arabinofuranosidase (EC 3.2.1.55) AbfA, 662 aa 4 3 19 8 [Reporter]SCO3910 (15D6)_PCR/5947 [Gene]SCO3910/10833 NC_003888 SCH24.32c, putative membrane protein, len: 448 aa; similar to many e.g. SW:DINF_ECOLI (EMBL:L02362), DinF, Escherichia coli DNA-damage-inducible protein (459 aa), fasta scores; opt: 571 z-score: 655.4 E(): 3.4e-29, 30.0% identity in 437 aa overlap. There is an alternative start codon at the third amino acid. Contains possible hydrophobic membrane spanning regions 1 3 7 20 [Reporter]SCO4138 (3A6)_PCR/4183 [Gene]SCO4138/7613 NC_003888 SCD84.05, pitH, phosphate transport protein, len: 332 aa; similar to N-terminal region of SW:PITH_STRHA (EMBL:L05390) Streptomyces halstedii putative low-affinity inorganic phosphate transporter (fragment), 213 aa; fasta scores: opt: 768 z-score: 850.1 E(): 0; 59.8% identity in 189 aa overlap, to TR:O30499 (EMBL:AF008187) Sinorhizobium meliloti phosphate transport protein, Pit, 334 aa; fasta scores: opt: 988 z-score: 1087.8 E(): 0; 48.3% identity in 327 aa overlap and to TR:CAB59459 (EMBL:AL132644) Streptomyces coelicolor putative secreted protein SCI8.28, 423 aa; fasta scores: opt: 991 z-score: 986.9 E(): 0; 45.9% identity in 331 aa overlap. Contains Pfam match to entry PF01384 PHO4, Phosphate transporter family. Contains also possible hydrophobic membrane spanning regions 4 3 19 7 [Reporter]SCO0693 (16P2)_PCR/5946 [Gene]SCO0693/10831 NC_003888 SCF42.03, possible membrane protein, len: 115 aa. Contains a possible N-terminal signal sequence and a possible membrane spanning hydrophobic domain. Rich in Ala and Leu. 1 3 7 19 [Reporter]SCO7202 (4M2)_PCR/4182 [Gene]SCO7202/7611 NC_003888 SC2H12.01, possible membrane protein, partial CDS, len: >87 aa. Contains a possible membrane spanning hydrophobic domain.,SC1D2.05, possible membrane protein, partial CDS, len >191 aa. Contains possible membrane spanning hydrophobic domains. Overlaps and extends into CDS SC2H12.01 on the adjoining cosmid. 4 1 13 4 [Reporter]SCP1.225 (18L20)_PCR/1779 [Gene]SCP1.225/3259 NC_003888 SCP1.225, unknown, len: 117aa; previously partially sequenced and annotated as TR:Q9JN71 (EMBL:AJ276673). Note possible alternative downstream translational start sites. 1 3 7 18 [Reporter]SCO6843 (5I2)_PCR/4181 [Gene]SCO6843/7609 NC_003888 SC3D9.11c, unknown, len: 372 aa. Weakly similar to Streptomyces coelicolor TR:Q9X8A4 (EMBL:AL049645) hypothetical protein, SCE2.09 (386 aa), fasta scores opt: 559 z-score: 616.2 E(): 6.5e-27; 37.5% identity in 363 aa overlap 4 3 19 5 [Reporter]SCO3224 (18H2)_PCR/5944 [Gene]SCO3224/10827 NC_003888 SCE8.17c, probable ABC transporter ATP-binding protein, len: 317aa; similar to many egs. TR:O86629 (EMBL:AL031155) ABC transporter ATP-binding protein from Streptomyces coelicolor (312 aa) fasta scores; opt: 1080, z-score: 1174.5, E(): 0, (57.4% identity in 303 aa overlap) and SW:BCRA_BACLI bacitracin-resistance ABC transporter from Bacillus licheniformis (306 aa) fasta scores; opt: 567, z-score: 621.0, E(): 2.8e-27, (42.4% identity in 217 aa overlap). N-terminal region previously sequenced therefore identical to TR:P72389 (EMBL:U51332) partial, designated D7 (103 aa) fasta scores; opt: 666, z-score: 734.8, E(): 0, (99.0% identity in 103 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, score 151.20, E-value 1.9e-41 and PS00017 ATP/GTP-binding site motif A (P-loop). 1 3 7 17 [Reporter]SCO6628 (6E2)_PCR/4180 [Gene]SCO6628/7607 NC_003888 SC4G2.02c, unknown, len: 196 aa; similar to S. coelicolor TR:Q53897 (EMBL:X60316) abaA gene ORFA (192 aa), fasta scores; opt: 245 z-score: 250.4 E(): 1.1e-06, 32.9% identity in 164 aa overlap. Also similar to SC10A5.20 (32.7% identity in 147 aa overlap) and SC7C7.02c (38.7% identity in 150 aa overlap) and to TR:Q9RKV5 (EMBL:AL132824) Streptomyces coelicolor putative regulatory protein SCAH10.02, 160 aa; fasta scores: opt: 436 Z-score: 526.8 E(): 1e-21; 51.449% identity in 138 aa overlap 4 3 19 4 [Reporter]SCO1367 (19D2)_PCR/5943 [Gene]SCO1367/10825 NC_003888 SC10A9.09c, probable ABC transport system ATP-binding protein, len: 251 aa; similar to SW:LOLD_ECOLI (EMBL:AE000212) Escherichia coli lipoprotein releasing system ATP-binding protein LolD, 233 aa; fasta scores: opt: 530 z-score: 576.0 E(): 1.2e-24; 40.8% identity in 218 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 3 7 16 [Reporter]SCO0301 (7A2)_PCR/4179 [Gene]SCO0301/7605 NC_003888 SC5G9.10, possible lipoprotein, len: 342 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition. Contains a probable N-terminal signal sequence and an appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 4 3 19 2 [Reporter]SCO5992 (20H22)_PCR/5941 [Gene]SCO5992/10823 NC_003888 SCBAC16H6.27, possible integral membrane protein, len: 164aa: no significant database matches. Contains possible membrane-spanning hydrophobic regions. 1 3 7 15 [Reporter]SCO6964 (7M22)_PCR/4178 [Gene]SCO6964/7603 NC_003888 SC6F7.17c, unknown, len: 216 aa. Similar to Mycobacterium tuberculosis TR:P95145(EMBL:Z83859) hypothetical 23.1 kd protein (211 aa), fasta scores opt: 519 z-score: 613.8 E(): 9.4e-27 46.6% identity in 193 aa overlap 4 3 18 22 [Reporter]SCO1118 (1H18)_PCR/5939 [Gene]SCO1118/10821 NC_003888 2SCG38.11c, possible integral membrane protein, len: 496 aa; low similarity to TR:AAG03843 (EMBL:AE004483) Pseudomonas aeruginosa conserved hypothetical protein PA0454, 733 aa; fasta scores: opt: 224 z-score: 245.0 E(): 3.3e-06; 28.0% identity in 300 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 7 13 [Reporter]SCO6059 (9E22)_PCR/4176 [Gene]SCO6059/7601 NC_003888 SC9B1.06, unknown, len: 181 aa 4 1 12 20 [Reporter]SCO5882 (3H12)_PCR/1773 [Gene]SCO5882/3249 NC_003888 SC3F7.02c, redV, function unknown, len: 395 aa 1 3 19 10 [Reporter]SCO3269 (13I6)_PCR/4437 [Gene]SCO3269/8085 NC_003888 SCE39.19, possible gntR-family regulator, len: 251 aa; some similarity to many egs. TR:Q07191 (EMBL:AJ005260) KorSA protein from the integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens (259 aa) fasta scores; opt: 327, z-score: 380.9, E(): 6.7e-14, (31.0% identity in 252 aa overlap) and TR:O86763 (EMBL:AL031035) KorSA transcriptional regulator from Streptomyces coelicolor cosmid 6A9 (259 aa) fasta scores; opt: 326, z-score: 379.7, E(): 7.7e-14, (28.9% identity in 249 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family. Also contains probable helix-turn-helix (+4.51 SD) 31-52aa. 1 3 19 9 [Reporter]SCO2977 (14E6)_PCR/4436 [Gene]SCO2977/8083 NC_003888 SCE50.05, unknown, len: 399 aa 1 3 19 8 [Reporter]SCO2607 (15A6)_PCR/4435 [Gene]SCO2607/8081 NC_003888 SCC88.18c, sfr, Sfr protein, len: 372 aa; identical to previously sequenced TR:O33623 (EMBL:Y14206) Streptomyces coelicolor Sfr protein and similar to SW:SP5E_BACSU (EMBL:X51419) Bacillus subtilis stage V sporulation protein E, SpoVE, 366 aa; fasta scores: opt: 795 z-score: 897.2 E(): 0; 39.4% identity in 363 aa overlap. Contains Pfam match to entry PF01098 FTSW_RODA_SPOVE, Cell cycle protein and match to Prosite entry PS00428 Cell cycle proteins ftsW / rodA / spoVE signature. Contains also possible hydrophobic membrane spanning regions 1 3 19 7 [Reporter]SCO3339 (16M2)_PCR/4434 [Gene]SCO3339/8079 NC_003888 SCE7.06c, probable ABC transporter ATP-binding subunit, len: 276aa; similar to many eg. TR:O33189 (EMBL:Z98268) ABC-type transport protein from Mycobacterium tuberculosis (255 aa) fasta scores; opt: 811, z-score: 850.0, E(): 0, (53.8% identity in 251 aa overlap) and TR:Q53626 (EMBL:U43537) ABC protein in mithramycin resistance (320 aa) fasta scores; opt: 491, z-score: 517.8, E(): 1.6e-21, (37.0% identity in 230 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and Prosite matches to PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). 1 4 7 21 [Reporter]SCO0784 (2E5)_PCR/6199 [Gene]SCO0784/11297 NC_003888 3SCF60.16, possible secreted protein, len: 227 aa. Contains possible N-terminal region signal peptide sequence 1 3 19 6 [Reporter]SCO0042 (17I2)_PCR/4433 [Gene]SCO0042/8077 NC_003888 SCJ4.23, unknown, len: 311 aa 1 4 7 20 [Reporter]SCO6515 (3A5)_PCR/6198 [Gene]SCO6515/11295 NC_003888 SC1E6.24c, pfpI, probable protease, len: 180 aa; similar to e.g. PFPI_PYRFU protease I (EC 3.2.-.-) (166 aa), fasta scores; opt: 413 z-score: 411.9 E(): 1.2e-15, 41.3% identity in 172 aa overlap 1 3 19 5 [Reporter]SCO1618 (18E2)_PCR/4432 [Gene]SCO1618/8075 NC_003888 SCI41.01c, hypothetical protein, len: >212 aa. Low similarity with C-terminus of TR:BAA80034 (EMBL:AP000060) Aeropyrum pernix 493 aa hypothetical protein APE1049 (493 aa); fasta scores: opt: 183 z-score: 217.5 E(): 9.6e-05; 29.2% identity in 202 aa overlap. Overlaps and extends StI35.40c,SCI35.40c, unknown. partial CDS, len: >68 aa 1 4 7 19 [Reporter]SCO6036 (4M1)_PCR/6197 [Gene]SCO6036/11293 NC_003888 SC1C3.24, unknown, len: 189 aa 1 3 19 4 [Reporter]SCO1664 (19A2)_PCR/4431 [Gene]SCO1664/8073 NC_003888 SCI52.06, conserved hypothetical protein, len: 265 aa; similar to TR:O06242 (EMBL:Z95388) Mycobacterium tuberculosis hypothetical 28.3 kDa protein MTCY270.35, 262 aa; fasta scores: opt: 495 Z-score: 595.6 bits: 117.9 E(): 1.4e-25; 50.951% identity in 263 aa overlap 1 4 7 18 [Reporter]SCO5466 (5I1)_PCR/6196 [Gene]SCO5466/11291 NC_003888 SC3D11.23c, possible hydrolase, len: 185aa; similar to many eg. SW:P34020 (LYC_CLOAB) autolytic lysozyme from Clostridium acetobutylicum (324 aa) fasta scores; opt: 231, z-score: 295.8, E(): 4.6e-09, 29.3% identity in 188 aa overlap. 1 3 19 3 [Reporter]SCP1.202 (19M22)_PCR/4430 [Gene]SCP1.202/8071 NC_003888 SCP1.202, unknown, len: 167aa; 1 3 19 2 [Reporter]SCO0978 (20E22)_PCR/4429 [Gene]SCO0978/8069 NC_003888 SCBAC19F3.05c, L-aspartate-alpha-decarboxylase, len: 139 aa: similar to many e.g. SW:P31664 (PAND_ECOLI) L-aspartate-alpha-decarboxylase PanD from Escherichia coli (126 aa) fasta scores; opt: 391, Z-score: 469.1, 50.877% identity (50.877% ungapped) in 114 aa overlap and SW:O06281 (PAND_MYCTU) aspartate 1-decarboxylase PanD from Mycobacterium tuberculosis (139 aa) fasta scores; opt: 572, Z-score: 677.0, 74.167% identity (74.167% ungapped) in 120 aa overlap. Contains Pfam match to entry PF02261 Asp_decarbox, Aspartate decarboxylase. 1 4 7 16 [Reporter]SCO4860 (7A1)_PCR/6194 [Gene]SCO4860/11287 NC_003888 SCK20.01, probable secreted hydrolase (fragment), len: >465 aa; similar to C-terminal region of SW:HEXA_DICDI (EMBL:J04065) Dictyostelium discoideum beta-hexosaminidase A precursor (EC 3.2.1.52) NagA, 532 aa; fasta scores: opt: 343 z-score: 387.7 E(): 4.6e-14; 25.4% identity in 393 aa overlap. Contains Pfam match to entry PF00728 Glyco_hydro_20, Glycosyl hydrolase family 20,SC5G8.28, partial CDS, possible secreted protein, len: >94aa; contains a possible cleavable N-terminal signal sequence. 1 3 18 22 [Reporter]SCO1115 (1E18)_PCR/4427 [Gene]SCO1115/8067 NC_003888 2SCG38.08, hypothetical protein, len: 332 aa; similar to TR:O50524 (EMBL:AL009204) Streptomyces coelicolor hypothetical 34.4 kDa protein SC9B10.17, 330 aa; fasta scores: opt: 263 z-score: 269.2 E(): 1.5e-07; 29.2% identity in 329 aa overlap and to TR:Q9EWI7 (EMBL:AL450223) Streptomyces coelicolor hypothetical protein SC7H9.09c, 332 aa; fasta scores: opt: 619 Z-score: 612.5 E(): 1.7e-26; 45.289% identity in 329 aa overlap 1 4 7 15 [Reporter]SCO6956 (7M21)_PCR/6193 [Gene]SCO6956/11285 NC_003888 SC6F7.09, possible oxidoreductase, len: 579 aa. Similar to many including: Rhodobacter capsulatus SW:NUON_RHOCA(EMBL:U25800) NADH dehydrogenase I chain N (EC 1.6.5.3) (478 aa), fasta scores opt: 538 z-score: 523.8 E(): 9.6e-22 29.1% identity in 385 aa overlap. Also similar to Bacillus firmus multiple resistance and pH regulation related protein D (493 aa), fasta scores opt: 1171 z-score: 1130.8 E(): 0 43.8% identity in 441 aa overlap. Also weakly similar to CDS SC6F7.07 also carried on this cosmid, fasta scores opt: 476 z-score: 387.6 E(): 4.5e-16 28.2% identity in 444 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a Pfam match to entry PF00361 oxidored_q1, NADH-Ubiquinone/plastoquinone (complex I), various chains. Note contains a 3xGSGEAS repeat towards the C-terminus. 1 3 18 21 [Reporter]SCO5775 (2A18)_PCR/4426 [Gene]SCO5775/8065 NC_003888 SC4H8.14c, gluC, probable glutamate permease, len: 222; similar to many members of the HisMQ subfamily of the binding-protein dependent transporters eg. GLUC_CORGL P48244 glutamate transport system permease protein from Corynebacterium glutamicum (225 aa), fasta scores; opt: 730 z-score: 1209.4 E(): 0, 58.8% identity in 199 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature 1 4 7 14 [Reporter]SCO7184 (8I21)_PCR/6192 [Gene]SCO7184/11283 NC_003888 SC8A11.12, probable branched amino acid transport system permease, len: 349 aa; similar to SW:LIVM_ECOLI (EMBL:J05516) Escherichia coli high-affinity branched-chain amino acid transport system permease, LivM, 425 aa; fasta scores: opt: 254 z-score: 285.7 E(): 1.9e-08; 28.1% identity in 360 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 18 20 [Reporter]SCO6808 (3M14)_PCR/4425 [Gene]SCO6808/8063 NC_003888 SC1A2.17c, possible ArsR-family transcriptional regulator, len: 120 aa. Similar to several regulators including: Streptomyces coelicolor TR:Q9X8X8 (EMBL:AL078610) putative transcriptional regulator, SCH35.28C (122 aa), fasta scores opt: 297 z-score: 392.7 E(): 1.8e-14 49.4% identity in 87 aa overlap and Mycobacterium tuberculosis TR:P71941 (EMBL:Z80225) putative transcriptional regulator (126 aa), fasta scores opt: 262 z-score: 348.0 E(): 5.6e-12 38.1% identity in 113 aa overlap. Contains a Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family with the putative helix-turn-helix motif situated between residues 56..77 (+4.54 SD). 1 4 7 13 [Reporter]SCO7171 (9E21)_PCR/6191 [Gene]SCO7171/11281 NC_003888 SC9A4.33c, possible lyase, len: 143 aa; similar to TR:O86701 (EMBL:AL031515) Streptomyces coelicolor putative lyase SC5C7.04, 138 aa; fasta scores: opt: 119 z-score: 158.2 E(): 0.24; 30.6% identity in 134 aa overlap 1 3 18 19 [Reporter]SCO7206 (4I14)_PCR/4424 [Gene]SCO7206/8061 NC_003888 SC2H12.05, possible membrane protein, len: 366 aa. Contains possible membrane spanning hydrophobic domains. 1 3 18 18 [Reporter]SCO2379 (5E14)_PCR/4423 [Gene]SCO2379/8059 NC_003888 SC4A7.07, possible acetyltransferase, len: 155 aa; similar to TR:Q55840 (EMBL:D64004) Synechocystis sp hypothetical 23.4 kD protein, 204 aa; fasta scores: opt: 134 z-score: 171.7 E(): 0.035; 26.6% identity in 124 aa overlap and to SW:RIMI_ECOLI (EMBL:X06117) Escherichia coli ribosomal-protein-alanine acetyltransferase (EC 2.3.1.128) RimI, 148 aa; fasta scores: opt: 115 z-score: 151.3 E(): 0.48; 34.5% identity in 55 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 4 7 11 [Reporter]SCO4525 (11M17)_PCR/6189 [Gene]SCO4525/11277 NC_003888 SCD35.32, unknown, len: 293 aa. Contains match to Prosite entry PS00038 Myc-type, 'helix-loop-helix' dimerization domain signature 1 3 18 17 [Reporter]SCO7756 (6A14)_PCR/4422 [Gene]SCO7756/8057 NC_003888 SC5E9.04, unknown, len: 74 aa 1 4 7 10 [Reporter]SCO2907 (12I17)_PCR/6188 [Gene]SCO2907/11275 NC_003888 SCE19A.07, possible PTS transmembrane component, len: 416 aa; similar to many PTS (phosphoenolpyruvate-dependent sugar phosphotransferase system) transmembrane and sugar-binding components e.g. SW:PTGB_ECOLI (EMBL:J02618), ptsG, glcA, Escherichia coli PTS system, glucose-specific IIBC component (477 aa), fasta scores; opt: 1112 z-score: 1244.4 E(): 0, 44.7% identity in 396 aa overlap. Similar to the adjacent CDS SCE19A.06, possible PTS transmembrane component (431 aa) (64.1% identity in 429 aa overlap). The homologous PTS family proteins have a C-terminal extension not present in this CDS. Contains hydrophobic, possible membrane-spanning regions 1 3 18 15 [Reporter]SCO6005 (8I10)_PCR/4420 [Gene]SCO6005/8055 NC_003888 SC7B7.02, possible lipoprotein, len: 469; has similarity to signal sequence of Mycobacterium leprae hypothetical protein TR:E334742 (EMBL:Z98604) MLCB2052.27 (445 aa), fasta scores; opt: 75 z-score: 199.2 E(): 0.00062, 23.3% identity in 335 aa overlap. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 1 4 7 9 [Reporter]SCO1767 (13E17)_PCR/6187 [Gene]SCO1767/11273 NC_003888 SCI51.07c, possible DNA hydrolase, len: 239 aa; shows weak similarity to nucleotide pyrophosphatases e.g. TR:Q58549 (EMBL:U67557) Methanococcus jannaschii ADP-ribose pyrophosphatase (169 aa), fasta scores; opt: 256 z-score: 312.0 E(): 5e-10, 43.8% identity in 112 aa overlap and SW:MUTX_STRMU (EMBL:D78182), MutX, Streptococcus mutans mutator MutT protein (dGTP pyrophosphohydrolase) (159 aa) (30.5% identity in 118 aa overlap). Also similar many hypothetical proteins e.g. TR:O69994 (EMBL:AL022374) S.coelicolor possible DNA hydrolase (294 aa) (56.2% identity in 249 aa overlap) and TR:O06597 (EMBL:Z95586) Mycobacterium tuberculosis hypothetical protein (236 aa) (34.8% identity in 221 aa overlap). Contains Pfam match to entry PF00293 mutT, Bacterial mutT protein and PS00893 mutT domain signature 1 3 18 14 [Reporter]SCO5506 (9E10)_PCR/4419 [Gene]SCO5506/8053 NC_003888 SC8D9.18, possible regulatory protein, len: 1091 aa; limited similarity to many proteins associated with regulatory functions e.g. C-terminal similarity with C-terminal of TR:O86603 (EMBL:AL031155) putative transcriptional regulator from Streptomyces coelicolor (892 aa) fasta scores; opt: 245, z-score: 254.6, E(): 7.1e-07, (30.6% identity in 451 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and a probable helix-turn-helix at 1036-1057aa (+3.98 SD). Also contains Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 82.70, E-value 7.8e-21. 1 4 7 8 [Reporter]SCO0795 (14A17)_PCR/6186 [Gene]SCO0795/11271 NC_003888 SCF43.06, unknown, len: 164 aa. Highly similar to a protein of unknown function from Streptomyces coelicolor SC1G2.10 (165 aa), fasta scores opt: 663 z-score: 732.7 E():0 60.1% identity in 163 aa overlap 1 3 18 13 [Reporter]SCO7367 (10A10)_PCR/4418 [Gene]SCO7367/8051 NC_003888 SC9H11.21c, probable membrane efflux protein, len: 446 aa. Similar to many proteins involved in arsenical resistance e.g. Staphylococcus xylosus SW:ARSB_STAXY(EMBL:M80565) arsenical pump membrane protein ArsB (429 aa), fasta scores opt: 255 z-score: 240.6 E(): 6.1e-06 24.1% identity in 440 aa overlap and Mycobacterium tuberculosis TR:P96860(EMBL:Z92774) probable arsenical pump membrane protein (413 aa), fasta scores opt: 730 z-score: 667.0 E(): 1.1e-29 53.7% identity in 445 aa overlap. Contains possible N-terminal region signal sequence peptide and multiple possible membrane spanning hydrophobic domains. 1 3 18 12 [Reporter]SCO4380 (11M6)_PCR/4417 [Gene]SCO4380/8049 NC_003888 SCD10.12, probable acetyl/propionyl CoA carboxylase, beta subunit, len: 532 aa; similar to TR:O86318 (EMBL:Z79700) Mycobacterium tuberculosis AccD2, 529 aa; fasta scores: opt: 2691 z-score: 3002.4 E(): 0; 75.5% identity in 530 aa overlap and to TR:AAF35258 (EMBL:AF059510) Arabidopsis thaliana 3-methylcrotonyl-CoA carbosylase non-biotinylated subunit (EC 6.4.1.4) MccB, 587 aa; fasta scores: opt: 1186 z-score: 1323.2 E(): 0; 48.0% identity in 546 aa overlap. Contains Pfam match to entry PF01039 Carboxyl_trans, Carboxyl transferase domain 1 4 7 6 [Reporter]SCO0266 (16I13)_PCR/6184 [Gene]SCO0266/11267 NC_003888 SCF1.08c, possible transcriptional regulator, len: 325aa; similar to many eg. TR:CAB43482 (EMBL:Y18527) PobR regulator from Pseudomonas sp. strain HR199 (293 aa) fasta scores; opt: 254, z-score: 302.3, E(): 1.8e-09, (30.3% identity in 254 aa overlap). Also similar to TR:O88020 (EMBL:AL031107) putative transcriptional regulator from Streptomyces coelicolor (301 aa) fasta scores; opt: 318, z-score: 376.7, E(): 1.3e-13, (37.2% identity in 253 aa overlap). Contains Pfam match to entry PF00165 HTH_2, Bacterial regulatory helix-turn-helix proteins, araC family. 1 3 18 11 [Reporter]SCO3951 (12I6)_PCR/4416 [Gene]SCO3951/8047 NC_003888 SCD78.18c, unknown, len: 320 aa; similarity to TR:Q54379 (EMBL:X79146) LmbY(295 aa), part of a gene cluster involved in lincomycin biosynthesis /resistance in Streptomyces lincolnensis, fasta scores; opt: 814, z-score: 584.1, E(): 2.8e-25, (43.8% identity in 290 aa overlap). Also similar to TR:Q49598 (EMBL:U31567) F420-dependent N5,N10-methylenetetrahydromethanopterin reductase from Methanopyruskandleri fasta scores; opt: 191, z-score: 333.9, E(): 2.5e-11, (29.1% identity in 247 aa overlap) 1 4 7 5 [Reporter]SCO3671 (17E13)_PCR/6183 [Gene]SCO3671/11265 NC_003888 SCH44.11c, dnaK, heat shock protein 70 (fragment), len: >515 aa; identical to C-terminal region of previously sequenced SW:DNAK_STRCO (EMBL:L46700) Streptomyces coelicolor DnaK protein (heat shock protein 70) 618 aa. Contains Pfam match to entry PF00012 HSP70, Hsp70 protein and matches to Prosite entries PS00329 Heat shock hsp70 proteins family signature 2 and PS01036 Heat shock hsp70 proteins family signature 3. Contains also possible colied-coli region at aprox. residues 388..402,SCH35.53, DnaK, heat shock protein 70, len: >135aa; previously sequenced therefore identical to SW:DNAK_STRCO. Contains Prosite match to PS00297 Heat shock hsp70 proteins family signature 1. 1 3 18 10 [Reporter]SCO3074 (13E6)_PCR/4415 [Gene]SCO3074/8045 NC_003888 SCE25.15c, possible integral membrane protein, len: 195 aa. Contains possible hydrophobic membrane spanning regions 1 4 7 4 [Reporter]SCO1688 (18A13)_PCR/6182 [Gene]SCO1688/11263 NC_003888 SCI30A.09, putative membrane protein, len: 113 aa; similar to SW:YU38_MYCTU hypothetical protein from Mycobacterium tuberculosis (122 aa) fasta scores; opt: 105, z-score: 141.8, E(): 1.4, (34.2% identity in 120 aa overlap). Contains possible hydrophobic membrane spanning regions 1 3 18 9 [Reporter]SCO2944 (14A6)_PCR/4414 [Gene]SCO2944/8043 NC_003888 SCE59.03c, possible sugar transporter membrane protein, len: 281 aa; similar to TR:CAB59590 (EMBL:AL132662) Streptomyces coelicolor putative sugar transporter inner membrane protein SCF11.13, 285 aa; fasta scores: opt: 730 z-score: 883.7 E(): 0; 42.1% identity in 278 aa overlap and to TR:AAF11008 (EMBL:AE001988) Deinococcus radiodurans ABC transporter, permease protein, MalFG family DR1436, 283 aa; fasta scores: opt: 645 z-score: 781.7 E(): 0; 38.2% identity in 272 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains possible hydrophobic membrane spanning regions 1 4 7 3 [Reporter]SCO6111 (19M9)_PCR/6181 [Gene]SCO6111/11261 NC_003888 SCBAC1A6.35c, probable oligopeptide ABC transporter ATP-binding protein, len: 585 aa; similar to SW:YLIA_ECOLI (EMBL:AE000185) Escherichia coli hypothetical ABC transporter ATP-binding protein YliA, 629 aa; fasta scores: opt: 1480 z-score: 1352.7 E(): 0; 44.5% identity in 580 aa overlap and N- and C-terminal regions both similar to SW:OPPD_BACSU (EMBL:X56347) Bacillus subtilis oligopeptide transport ATP-binding protein OppD, 358 aa; fasta scores: opt: 864 z-score: 796.4 E(): 0; 49.6% identity in 274 aa overlap. Contains 2x Pfam matches to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries 2x PS00017 ATP/GTP-binding site motif A (P-loop) and 2x PS00211 ABC transporters family signature 1 3 18 8 [Reporter]SCO1698 (15M2)_PCR/4413 [Gene]SCO1698/8041 NC_003888 SCI30A.19, unknown, len: 153 aa; similar to many of undefined function e.g. TR:P96807 (EMBL:Z92770) hypothetical protein from Mycobacterium tuberculosis (151 aa) fasta scores; opt: 639, z-score: 785.9, E(): 0, (60.8% identity in 148 aa overlap) and SW:NODN_RHILV nodulation protein from Rhizobium leguminosarum (161 aa) fasta scores; opt: 388, z-score: 482.2, E(): 1.6e-19, (44.8% identity in 145 aa overlap) 1 3 18 7 [Reporter]SCO3721 (16I2)_PCR/4412 [Gene]SCO3721/8039 NC_003888 SCH35.03, probable carbonic anhydrase, len: 207aa; similar to many (both prokayote and eukaryote) egs. TR:Q39589 (EMBL:U41189) an algal mitochondrial carbonic anhydrase from Chlamydomonas reinhardtii (267 aa) fasta scores; opt: 431, z-score: 502.8, E(): 1.1e-20, (37.9% identity in 198 aa overlap) and SW:CYNT_ECOLI carbonic anhydrase from Escherichia coli (219 aa) fasta scores; opt: 227, z-score: 271.0, E(): 9.1e-08, (30.9% identity in 178 aa overlap). Contains Prosite match to PS00704 Prokaryotic-type carbonic anhydrases signature 1 and Pfam match to entry PF00484 Pro_CA, Prokaryotic-type carbonic anhydrases. 1 4 6 22 [Reporter]SCO5381 (1E5)_PCR/6178 [Gene]SCO5381/11257 NC_003888 2SC6G5.25c, conserved hypothetical protein, len: 190aa; similar to many eg. SW:Q10622 (Y02Y_MYCTU) hypothetical protein from Mycobacterium tuberculosis (193 aa) fasta scores; opt: 800, z-score: 984.0, E(): 0, 65.6% identity in 189 aa overlap. Contains Pfam match to entry PF01923 DUF80, Protein of unknown function. 1 3 18 6 [Reporter]SCO3830 (17E2)_PCR/4411 [Gene]SCO3830/8037 NC_003888 SCGD3.31c, bkdB2, probable branched-chain alpha keto acid dehydrogenase E1 beta subunit, len: 334 aa; similar to E1 beta subunits from pyruvate dehydrogenase complexes and branched-chain alpha keto acid dehydrogenases e.g. TR:Q53593 (EMBL:U1716), BkdB, Streptomyces avermitilis E1-beta branched-chain alpha keto acid dehydrogenase (334 aa), fasta scores; opt: 2063 z-score: 2427.3 E(): 0, 91.0% identity in 334 aa overlap and SW:ODPB_BACST (EMBL:X53560), PdhB, Bacillus stearothermophilus pyruvate dehydrogenase complex E1 beta subunit (324 aa) (45.5% identity in 319 aa overlap). Highly similar to SCGD3.17c, probable branched-chain alpha keto acid dehydrogenase E1 beta subunit (326 aa) (55.2% identity in 324 aa overlap), in a duplication of this region downstream 1 4 6 21 [Reporter]SCO0778 (2A5)_PCR/6177 [Gene]SCO0778/11255 NC_003888 3SCF60.10, possible integral membrane protein, len: 102 aa. Contains possible hydrophobic membrane spanning regions 1 3 18 5 [Reporter]SCO0503 (18A2)_PCR/4410 [Gene]SCO0503/8035 NC_003888 SCF34.22, hypothetical protein, len: 326 aa; unknown function, shows weak similarity to the N-terminal half of SCH10.22C (EMBL:AL049754) Streptomyces coelicolor putative lipase/esterase (301 aa), fasta scores; opt: 178 z-score: 202.4 E(): 0.00065, 30.1% identity in 163 aa overlap 1 4 6 20 [Reporter]SCO1513 (3M1)_PCR/6176 [Gene]SCO1513/11253 NC_003888 SCL2.03c, relA, GTP pyrophosphokinase, len: 847 aa; identical to previously sequenced SW:RELA_STRCO (EMBL:X87267) Streptomyces coelicolor GTP pyrophosphokinase (EC 2.7.6.5) RelA, 847 aa and highly similar to SW:RELA_STRAT (EMBL:AF072829) Streptomyces antibioticus RelA protein, 841 aa; fasta scores: opt: 4969 z-score: 4906.2 E(): 0; 90.3% identity in 848 aa overlap and to SW:RELA_CORGL (EMBL:AF038651) Corynebacterium glutamicum GTP pyrophosphokinase (EC 2.7.6.5) RelA, 760 aa; fasta scores: opt: 1613 z-score: 1594.3 E(): 0; 58.7% identity in 774 aa overlap. Contains Pfam match to entry PF01842 ACT, ACT domain and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 18 4 [Reporter]SCP1.216 (18M22)_PCR/4409 [Gene]SCP1.216/8033 NC_003888 SCP1.216, unknown, len: 173aa; part of possible transposon comprising CDSs SCP1.14, SCP1.15 and SCP1.16. 1 4 6 19 [Reporter]SCO6032 (4I1)_PCR/6175 [Gene]SCO6032/11251 NC_003888 SC1C3.20c, possible hydrolase, len: 506 aa; weakly similar to many e.g. HEXA_ALTSO beta-hexosaminidase a precursor (598 aa), fasta scores; opt: 463 z-score: 613.4 E(): 6.2e-27, 29.7% identity in 526 aa overlap 1 3 18 3 [Reporter]SCO4014 (19I22)_PCR/4408 [Gene]SCO4014/8031 NC_003888 2SC10A7.18c, conserved hypothetical protein, len: 119 aa; similar to SW:YTFH_ECOLI (EMBL:U14003) Escherichia coli hypothetical 14.1 kDa protein in RplI-CpdB intergenic region YtfH, 126 aa; fasta scores: opt 376 z-score: 479.2 E(): 3.5e-19; 56.1% identity in 98 aa overlap. Contains Pfam match to entry PF01638 DUF24, Protein of unknown function DUF24 1 3 18 2 [Reporter]SCO3986 (20A22)_PCR/4407 [Gene]SCO3986/8029 NC_003888 SCBAC25E3.23, possible GntR-family transcriptional regulator, len: 243 aa: similar to TR:Q9X8D7 (EMBL:AL049573) putative GntR-family regulator from Streptomyces coelicolor (243 aa) fasta scores; opt: 1025, Z-score: 1169.6, 67.083% identity (67.083% ungapped) in 240 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and Prosite match to PS00043 Bacterial regulatory proteins, gntR family signature. 1 4 6 17 [Reporter]SCO7328 (6A1)_PCR/6173 [Gene]SCO7328/11247 NC_003888 SC4G10.07c, possible regulatory protein, len: 144 aa. Highly similar to Streptomyces fradiae TR:Q9Z9I5 (EMBL:AB024056) beta-lactamase regulator protein (139 aa), fasta scores opt: 530 z-score: 661.7 E(): 2.2e-29 61.5% identity in 122 aa overlap and Streptomyces coelicolor TR:Q9X7S3 (EMBL:AL049863) putative regulator protein, SC5H1.15C (151 aa), fasta scores opt: 178 z-score: 231.0 E(): 2.1e-05 30.8% identity in 130 aa overlap. 1 3 17 22 [Reporter]SCO1110 (1A18)_PCR/4405 [Gene]SCO1110/8027 NC_003888 2SCG38.03, probable secreted lyase, len: 266 aa; similar to TR:Q04701 (EMBL:M94691) Fusarium solani pectate lyase A precursor (EC 4.2.2.2) PelA, 242 aa; fasta scores: opt: 605 z-score: 664.1 E(): 1.5e-29; 43.6% identity in 236 aa overlap. Contains possible N-terminal region signal peptide sequence 1 4 6 16 [Reporter]SCO7316 (6M21)_PCR/6172 [Gene]SCO7316/11245 NC_003888 SC5F8.26, possible integral membrane protein, len: 280 aa. Similar to Bacillus subtilis SW:YXXB_BACSU(EMBL:D45912) hypothetical 31.8 kd protein (289 aa), fasta scores opt: 476 z-score: 539.7 E(): 1.4e-22 31.0% identity in 245 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 1 3 17 21 [Reporter]SCO5872 (2M14)_PCR/4404 [Gene]SCO5872/8025 NC_003888 SC2E9.13, kdpE, two-component trascriptional regulator, len: 227, similar to E.coli turgor pressure regulator KDPE_ECOLI P21866 kdp operon transcriptional regulatory protein (225 aa), fasta scores; opt: 752 z-score: 1090.9 E(): 0, 51.1% identity in 225 aa overlap. Contains Pfam match to entry trans_reg_C PF00486, Transcriptional regulatory proteins, C terminal, score 96.22 1 4 6 15 [Reporter]SCO6952 (7I21)_PCR/6171 [Gene]SCO6952/11243 NC_003888 SC6F7.05c, unknown, len: 347 aa. Weakly similar to several proteins of unknown function e.g. Mycobacterium tuberculosis SW:Y07U_MYCTU(EMBL:Z75555) (273 aa), fasta scores opt: 173 z-score: 212.9 E(): 0.0002 26.8% identity in 276 aa overlap. 1 3 17 20 [Reporter]SCO6804 (3I14)_PCR/4403 [Gene]SCO6804/8023 NC_003888 SC1A2.13, unknown, len: 127 aa. 1 4 6 14 [Reporter]SCO6889 (8E21)_PCR/6170 [Gene]SCO6889/11241 NC_003888 SC7F9.41, unknown, len: 246 aa. Similar to Streptomyces coelicolor TR:Q9X8A7 (EMBL:AL049645) hypothetical 28.5 KD protein, SCE2.12 (259 aa), fasta scores opt: 405 z-score: 450.6 E(): 1.1e-17 35.6% identity in 233 aa overlap. 1 3 17 19 [Reporter]SCO5485 (4E14)_PCR/4402 [Gene]SCO5485/8021 NC_003888 SC2A11.19, small hydrophobic membrane protein, len: 97 aa. Contains possible hydrophobic membrane spanning regions 1 3 17 18 [Reporter]SCO2375 (5A14)_PCR/4401 [Gene]SCO2375/8019 NC_003888 SC4A7.03, possible secreted protein, len: 406 aa; similar to various hypothetical proteins, e.g. TR:Q9X8R0 (EMBL:AL049754) Streptomyces coelicolor hypothetical 33.9 kD protein, SCH10.21c, 329 aa; fasta scores: opt: 634 z-score: 714.7 E(): 2e-32; 39.3% identity in 346 aa overlap. Contains possible N-terminal signal peptide sequence 1 4 6 12 [Reporter]SCO1953 (10M17)_PCR/6168 [Gene]SCO1953/11237 NC_003888 SCC54.13c, uvrC, ABC excision nuclease subunit C, len: 728aa; member of a conserved family eg, SW:UVRC_MYCTU uvrC, excinuclease ABC subunit C from Mycobacterium tuberculosis (646 aa) fasta scores; opt: 1516, z-score: 1657.1, E(): 0, (58.4% identity in 693 aa overlap) and SW:UVRC_BACSU uvrC, excinuclease ABC subunit C from Bacillus subtilis (598 aa) fasta scores; opt: 567, z-score: 619.9, E(): 3.2e-27, (34.0% identity in 653 aa overlap). 1 3 17 17 [Reporter]SCO6522 (6M10)_PCR/4400 [Gene]SCO6522/8017 NC_003888 SC5C7.07, unknown, len: 480 aa 1 4 6 11 [Reporter]SCO4521 (11I17)_PCR/6167 [Gene]SCO4521/11235 NC_003888 SCD35.28c, possible secreted protein, len: 361 aa; similar to TR:Q9F3F6 (EMBL:AL392175) Streptomyces coelicolor putative secreted protein 2SCD4.18, 355 aa; fasta scores: opt: 942 Z-score: 1049.8 E(): 7.7e-51; 43.296% identity. Contains possible N-terminal region signal peptide sequence 1 3 17 16 [Reporter]SCO6782 (7I10)_PCR/4399 [Gene]SCO6782/8015 NC_003888 SC6A5.31c, probable zinc-binding alcohol dehydrogenase, len: 363aa; similar to many eg. TR:Q59096 (EMBL:U61983) benzyl alcohol dehydrogenase from Acinetobacter calcoaceticus (371 aa) fasta scores; opt: 948, z-score: 937.8, E(): 0, (42.8% identity in 360 aa overlap). Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and Prosite match to PS00059 Zinc-containing alcohol dehydrogenases signature. 1 4 6 10 [Reporter]SCO4279 (12E17)_PCR/6166 [Gene]SCO4279/11233 NC_003888 SCD95A.12c, possible acetyltransferase, len: 156 aa; similar to TR:AAF22297 (EMBL:AF185569) Rattus norvegicus putative N-acetyltransferase camello 2, Cml2, 221 aa; fasta scores: opt: 125 z-score: 168.1 E(): 0.067 23.6% identity in 123 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 3 17 15 [Reporter]SCO5309 (8E10)_PCR/4398 [Gene]SCO5309/8013 NC_003888 SC6G9.24c, conserved hypothetical protein, len: 365 aa; unknown function, similar to hypothetical proteins from Mycobacterium tuberculosis, Bacillus subtilis and Archaeoglobus fulgidus e.g. TR:O05866 (EMBL:Z95209) Mycobacterium tuberculosis hypothetical protein (273 aa), fasta scores; opt: 648 z-score: 651.9 E(): 5.6e-29, 36.5% identity in 274 aa overlap. An alternative start codon is present as codon 4 1 4 6 9 [Reporter]SCO3856 (13A17)_PCR/6165 [Gene]SCO3856/11231 NC_003888 SCH69.26c, probable peptidyl-prolyl cis-trans isomerase, len: 177aa; similar to many egs. TR:P77949 (EMBL:U64692) peptidyl-prolyl cis-trans isomerase from Streptomyces chrysomallus (175 aa) fasta scores; opt: 950, z-score: 1136.4, E(): 0, (78.3% identity in 175 aa overlap) and TR:O54168 (EMBL:AL021411) peptidyl-prolyl isomerase from Streptomyces coelicolor (157 aa) fasta scores; opt: 400, z-score: 486.8, E(): 8.8e-20, (50.0% identity in 124 aa overlap). Contains Pfam match to entry PF00160 pro_isomerase, Peptidyl-prolyl cis-trans isomerase. 1 3 17 14 [Reporter]SCO5501 (9A10)_PCR/4397 [Gene]SCO5501/8011 NC_003888 SC8D9.13, probable Glu-tRNA-Gln amidotransferase subunit B, gatB, len: 504aa; similar to many eg. TR:O30509 (EMBL:AF008553) Glu-tRNA-Gln amidotransferase subunit B, gatB, from Bacillus subtilis (476 aa) fasta scores; opt: 886, z-score: 983.5, E(): 0, (42.9% identity in 485 aa overlap). 1 3 17 13 [Reporter]SCO2500 (10M6)_PCR/4396 [Gene]SCO2500/8009 NC_003888 SCC121.03, possible membrane protein, len: 142 aa 1 4 6 7 [Reporter]SCO3485 (15I13)_PCR/6163 [Gene]SCO3485/11227 NC_003888 SCE65.21c, probable lacI-family transcriptional regulator, len: 349 aa; similar to TR:AAD42741 (EMBL:AF105219) Thermomonospora curvata putative regulatory protein AglR, 347 aa; fasta scores: opt: 563 z-score: 648.1 E(): 1e-28; 34.9% identity in 341 aa overlap and to SW:CYTR_ECOLI (EMBL:X03683) Escherichia coli, transcriptional repressor CytR 341 aa; fasta scores: opt: 552 z-score: 635.6 E(): 5e-28; 30.5% identity in 347 aa overlap. Contains matches to Pfam entries PF00356 lacI, Bacterial regulatory proteins, lacI family PF00532 Peripla_BP_like, Periplasmic binding proteins and LacI. Contains probable helix-turn-helix motif at residues 18..39 (+4.84 SD) 1 3 17 12 [Reporter]SCO4376 (11I6)_PCR/4395 [Gene]SCO4376/8007 NC_003888 SCD10.08, unknown, len: 169 aa. Contains 3x degenerate repeat: T(T/A)P and a 2x conserved repeat TTDAA 1 4 6 6 [Reporter]SCO0257 (16E13)_PCR/6162 [Gene]SCO0257/11225 NC_003888 SCF20.03, hypothetical protein, len: 333 aa; similar to TR:Q9Y9P8 (EMBL:AP000063) hypothetical protein APE2240 from Aeropyrum pernix (324 aa) fasta scores; opt: 728, z-score: 908.5, E(): 0, (51.7% identity in 298 aa overlap) 1 3 17 11 [Reporter]SCO4805 (12E6)_PCR/4394 [Gene]SCO4805/8005 NC_003888 SCD63A.16, hypothetical protein, len: 348 aa; similar to SW:YEHP_ECOLI (EMBL:) Escherichia coli hypothetical 42.1 kD protein in MolR-BglX intergenic region YehP, 378 aa; fasta scores: opt: 721 z-score: 816.7 E(): 0; 35.7% identity in 378 aa overlap 1 4 6 5 [Reporter]SCO0065 (17A13)_PCR/6161 [Gene]SCO0065/11223 NC_003888 SCJ4.46, possible extracellular binding protein, part of a possible binding protein dependent transport system, len: 437 aa; similar to many egs. TR:Q9Z492 (EMBL:AF043654) BxlE, extracellular binding protein from Streptomyces lividans (434 aa) fasta scores; opt: 256, z-score: 290.5, E(): 8.1e-09, (23.3% identity in 446 aa overlap) and SW:YC14_PYRHO hypothetical ABC transporter extracellular binding protein from Pyrococcus horikoshii OT3 (441 aa) fasta scores; opt: 348, z-score: 393.3, E(): 1.5e-14, (25.4% identity in 343 aa overlap). Contains Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site so may be anchored by lipid. 1 3 17 10 [Reporter]SCO3069 (13A6)_PCR/4393 [Gene]SCO3069/8003 NC_003888 SCE25.10c, possible integral membrane protein, len: 114 aa. Contains possible hydrophobic membrane spanning regions 1 4 6 4 [Reporter]SCO3413 (18M9)_PCR/6160 [Gene]SCO3413/11221 NC_003888 SCE9.20c, tipA, transcriptional regulator, len: 253 aa; highly similar to many e.g. SW:TIPA_STRLI (EMBL:S64314), TipA, Streptomyces lividans thiostrepton-binding transcriptional activator (253 aa), fasta scores; opt: 1693 z-score: 1909.7 E(): 0, 99.6% identity in 253 aa overlap. Weakly similar to others from S. coelicolor e.g. TR:O54191 (EMBL:AL021411) probable transcriptional regulator (196 aa) (29.8% identity in 181 aa overlap). Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family, score 66.50, E-value 5.7e-1 and PS00552 Bacterial regulatory proteins, merR family signature. Contains probable coiled-coil from 77 to 107 (31 residues) (Max score: 1.523, probability 0.97) 1 3 17 9 [Reporter]SCO0269 (14M2)_PCR/4392 [Gene]SCO0269/8001 NC_003888 SCF1.11, unknown, len: 1053aa; similar to large proteins thought to be involved in the post-translational modification of lantibiotics eg. SW:SPAB_BACSU subtilin modification protein from Bacillus subtilis (1030 aa) fasta scores; opt: 346, z-score: 367.5, E(): 4.2e-13, (22.6% identity in 1041 aa overlap). 1 4 5 20 [Reporter]SCO5879 (3I1)_PCR/6154 [Gene]SCO5879/11211 NC_003888 SC2E9.20, redW, acyl-coa dehydrogenase, len: 391; similar to many eg. ACDB_BACSU P45857 acyl-coa dehydrogenase (ec 1.3.99.-) (379 aa), fasta scores; opt: 831 z-score: 1005.0 E(): 0, 40.7% identity in 371 aa overlap. Contains Pfam match to entry Acyl-CoA_dh PF00441, Acyl-CoA dehydrogenases, score 308.86 1 4 5 19 [Reporter]SCO6158 (4E1)_PCR/6153 [Gene]SCO6158/11209 NC_003888 SC1A9.22c, unknown, len: 281 aa 1 4 5 18 [Reporter]SCO7092 (5A1)_PCR/6152 [Gene]SCO7092/11207 NC_003888 SC3A4.18, possible secreted protein, len: 300 aa. Contains a possible N-terminal signal sequence. Contains a TTA/leucine codon, possible target for bldA regulation 1 4 5 17 [Reporter]SCO7052 (5M21)_PCR/6151 [Gene]SCO7052/11205 NC_003888 SC4G1.18c, unknown, len: 131 aa 1 4 5 16 [Reporter]SCO7311 (6I21)_PCR/6150 [Gene]SCO7311/11203 NC_003888 SC5F8.21c, probable amino acid decarboxylase, len: 492 aa. Similar to many e.g. Bacillus subtilis SW:DCLY_BACSU (EMBL:X58433) lysine decarboxylase (EC 4.1.1.18) (490 aa), fasta scores: opt: 1108 z-score: 1197.8 E():0 35.6% identity in 494 aa overlap. Contains a Pfam match to entry PF01276 OKR_DC_1, Orn/Lys/Arg decarboxylase. 1 4 5 15 [Reporter]SCO7421 (7E21)_PCR/6149 [Gene]SCO7421/11201 NC_003888 SC6D11.17c, cvnB10, unknown, len: 135 aa. Highly similar to many proteins of undefined function from Streptomyces coelicolor including: TR:CAB61281 (EMBL:AL132991) SCF55.11c (152 aa), fasta scores opt: 634 z-score: 769.3 E():0 71.9% identity in 128 aa overlap and TR:Q9X835(EMBL:AL049727) SC9B1.15C (139 aa), fasta scores opt: 329 z-score: 407.1 E(): 3e-15 40.9% identity in 132 aa overlap 1 3 7 12 [Reporter]SCO2426 (10A22)_PCR/4175 [Gene]SCO2426/7599 NC_003888 SCC42.07, possible regulatory protein, len: 403 aa; similar to TR:CAB88166 (EMBL:AL352956) Streptomyces coelicolor putative regulatory protein SC1H10.22c, 555 aa; fasta scores: opt: 157 z-score: 183.8 E(): 0.0091; 26.8% identity in 447 aa overlap. Contains a possible helix-turn-helix motif at residues 347..863 (+3.80 SD) and two TTA leucine codons (codon 170 and codon 174), possible targets for bldA regulation 1 3 7 11 [Reporter]SCO4611 (11M18)_PCR/4174 [Gene]SCO4611/7597 NC_003888 SCD39.11, unknown, len: 197 aa 1 3 7 10 [Reporter]SCO2916 (12I18)_PCR/4173 [Gene]SCO2916/7595 NC_003888 SCE19A.16c, conserved hypothetical protein, len: 105 aa; similar to SW:Y03G_MYCTU (EMBL:Z73902) Mycobacterium tuberculosis hypothetical protein (101 aa), fasta scores; opt: 377 z-score: 514.8 E(): 2.4e-21, 60.0% identity in 105 aa overlap and SW:Y03G_MYCLE (EMBL:U00014) Mycobacterium leprae hypothetical protein (94 aa) (70.3% identity in 74 aa overlap) 1 3 7 9 [Reporter]SCO2968 (13E18)_PCR/4172 [Gene]SCO2968/7593 NC_003888 SCE59.27c, possible cell division protein, len: 305 aa; similar to TR:P96293 (EMBL:X70031) Mycobacterium tuberculosis FtsX, 297 aa; fasta scores: opt: 591 z-score: 681.4 E(): 1.5e-30; 34.9% identity in 304 aa overlap and to SW:FTSX_ECOLI (EMBL:X04398) Escherichia coli cell division protein FtsX, 352 aa; fasta scores: opt: 194 z-score: 228.3 E(): 2.7e-05; 23.4% identity in 295 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 7 8 [Reporter]SCO0322 (14A18)_PCR/4171 [Gene]SCO0322/7591 NC_003888 SCF12.01c, possible ABC transport ATP-binding subunit, len: >230aa; similar to many eg. SW:TLRC_STRFR tylosin resistance ATP-binding protein from Streptomyces fradiae (548 aa) fasta scores; opt: 1017, z-score: 1131.8, E(): 0, (69.0% identity in 232 aa overlap). Contains Prosite match to PS00211 ABC transporters family signature and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop).,SC5G9.31c, probable ABC-transporter ATP-binding protein, partial CDS, len: >371 aa; similar ATP-binding components from antibiotic resistance export systems e.g. SW:TLRC_STRFR (EMBL:M57437), tlrC, Streptomyces fradiae tylosin resistance ATP-binding protein (548 aa), fasta scores; opt: 1517 z-score: 1597.3 E(): 0, 67.4% identity in 356 aa overlap. Also similar to many other putative ABC-transporter ATP-binding proteins. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 7 7 [Reporter]SCO3305 (15M14)_PCR/4170 [Gene]SCO3305/7589 NC_003888 SCE68.03, possible membrane protein, len: 441 aa; unknown function, weakly similar to TR:O66257 (EMBL:AB010415) Actinobacillus actinomycetemcomitans integral membrane acetyltransferase (344 aa), fasta scores; opt: 259 z-score: 247.1 E(): 1.9e-06, 24.2% identity in 360 aa overlap. Contains hydrophobic, possible membrane-spanning regions 1 3 7 6 [Reporter]SCO2615 (16I14)_PCR/4169 [Gene]SCO2615/7587 NC_003888 SCC88.26c, valS, valyl tRNA synthetase, len: 874 aa; C-terminal region identical to the previously sequenced SW:SYV_STRCO (EMBL:Y13070) Streptomyces coelicolor valyl-tRNA synhtetase (EC 6.1.1.9) ValS (fragment), 258 aa and similar to TR:Q9X2D7 (EMBL:AE001819) Thermotoga maritima valyl tRNA synthetase, 865 aa; fasta scores: opt: 2710 z-score: 3059.0 E(): 0; 47.7% identity in 874 aa overlap and to SW:SYV_BACST (EMBL:M16318) Bacillus stearothermophilus valyl-tRNA synthetase (EC 6.1.1.9) (valine-tRNA ligase) ValS, 880 aa; fasta scores: opt: 2596 z-score: 2930.0 E(): 0; 48.5% identity in 877 aa overlap. Contains Pfam match to entry PF00133 tRNA-synt_1, tRNA synthetases class I (I, L, M and V) and match to Prosite entry PS00178 Aminoacyl-transfer RNA synthetases class-I signature 1 3 7 5 [Reporter]SCO1052 (17E14)_PCR/4168 [Gene]SCO1052/7585 NC_003888 SCG20A.32c, possible regulatory protein, len: 222 aa; similar to C-terminal part of TR:CAB91114 (EMBL:AL355913) Streptomyces coelicolor putative regulatory protein SC5H4.02, 552 aa; fasta scores: opt: 195 z-score: 192.1 E(): 0.0032; 30.5% identity in 210 aa overlap. Contains possible helix-turn-helix motif at residues 172..193 (+3.29 SD) 1 3 7 4 [Reporter]SCO3163 (18A14)_PCR/4167 [Gene]SCO3163/7583 NC_003888 SCE87.14c, unknown, len: 91 aa. Similar to a hypothetical protein from Mycobacterium tuberculosis: SW:YQ32_MYCTU (EMBL:Z80225) hypothetical 10.1 KD protein (93 aa), fasta scores opt: 112 z-score: 161.8 E(): 0.12 31.3% identity in 83 aa overlap. 1 3 7 3 [Reporter]SCP1.182 (19M10)_PCR/4166 [Gene]SCP1.182/7581 NC_003888 SCP1.182, unknown, len: 251aa; 1 3 7 2 [Reporter]SCP1.258c (20E10)_PCR/4165 [Gene]SCP1.258c/7579 NC_003888 SCP1.258c, unknown, alanine-rich, len: 166aa; 4 3 18 10 [Reporter]SCO3077 (13H6)_PCR/5927 [Gene]SCO3077/10797 NC_003888 SCE25.18, probable cystathionine beta-synthase, len: 461 aa; highly similar to TR:O53425 (EMBL:AL021897) Mycobacterium tuberculosis cystathionine beta-synthase CysM, 464 aa; fasta scores: opt: 2025 z-score: 2184.8 E(): 0; 67.6% identity in 454 aa overlap, to TR:Q9YHU3 (EMBL:AF090120) Fugu rubripe cystathionine beta-synthase, 585 aa; fasta scores: opt: 1087 z-score: 1173.5 E(): 0; 39.6% identity in 465 aa overlap and to TR:CAB50991 (EMBL:AL096852) Streptomyces coelicolor putative cysteine synthase SCE19A.10c, 316 aa; fasta scores: opt: 558 z-score: 540.6 E(): 1.4e-24; 40.3% identity in 303 aa overlap. Contains Pfam matches to entries PF00291 S_T_dehydratase, Pyridoxal-phosphate dependent enzyme, 2x PF00571 CBS, CBS domain and match to Prosite entry PS00901 Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site 1 3 6 22 [Reporter]SCO5389 (1E6)_PCR/4163 [Gene]SCO5389/7577 NC_003888 2SC6G5.33, unknown, len: 130 aa 4 3 18 9 [Reporter]SCO1726 (14D6)_PCR/5926 [Gene]SCO1726/10795 NC_003888 SCI11.15, possible ATPase, len: 364 aa; unknown function, very weak similarity to eukaryotic putative ATPases e.g. TR:P78787 (EMBL:D89136) Schizosaccharomyces pombe putative ATPase (partial CDS) (419 aa), fasta scores; opt: 184 z-score: 216.6 E(): 9.9e-05, 26.5% identity in 309 aa overlap and to part of SW:AFG2_YEAST (EMBL:L14615), Agf2, Saccharomyces cerevisiae putative ATPase essential for viability (780 aa) (29.9% identity in 137 aa overlap). Contains weak Pfam match to entry PF00004 AAA, ATPases associated with various cellular activities (AAA) and PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 6 21 [Reporter]SCO0788 (2A6)_PCR/4162 [Gene]SCO0788/7575 NC_003888 3SCF60.20c, unknown, len: 78 aa 4 3 18 8 [Reporter]SCO3701 (15P2)_PCR/5925 [Gene]SCO3701/10793 NC_003888 SCH35.23c, unknown, len: 76aa; similar to the N-terminus of many thioredoxin reductases from prokaryotes and eukaryotes egs. SW:TRXB_STRCO TrxB, thioredoxin reductase from Streptomyces coelicolor (319 aa) fasta scores; opt: 215, z-score: 280.7, E(): 2.6e-08, (63.2% identity in 57 aa overlap) and SW:TRXB_NEUCR Cys-9 thioredoxin reductase from Neurospora crassa (334 aa) fasta scores; opt: 158, z-score: 209.4, E(): 0.00025, (51.8% identity in 56 aa overlap). 1 3 6 20 [Reporter]SCO1223 (3M2)_PCR/4161 [Gene]SCO1223/7573 NC_003888 2SCG58.23, rocD, ornithine aminotransferase, len: 407 aa; highly similar to SW:OAT_BACSU (EMBL:X81802) Bacillus subtilis ornithine aminotransferase (EC 2.6.1.13) RocD, 401 aa; fasta scores: opt: 1356 z-score: 1462.1 E(): 0; 50.4% identity in 395 aa overlap. Contains Pfam match to entry PF00202 aminotran_3, Aminotransferases class-III pyridoxal-phosphate and match to Prosite entry PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site 4 3 18 7 [Reporter]SCO3675 (16L2)_PCR/5924 [Gene]SCO3675/10791 NC_003888 SCH35.49c, putative conserved secreted protein, len: 410aa; Similar to others from Streptomyces coelicolor. Contains a possible N-terminal signal sequence. 1 3 6 19 [Reporter]SCO6040 (4I2)_PCR/4160 [Gene]SCO6040/7571 NC_003888 SC1C3.28c, probable lipoprotein, len: 327 aa; contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Contains possible hydrophobic membrane spanning region at C-terminal domain 1 3 6 18 [Reporter]SCO6340 (5E2)_PCR/4159 [Gene]SCO6340/7569 NC_003888 SC3A7.08, unknown, len: 211 aa; similar to hypothetical proteins e.g. S. coelicolor TR:O54167 (EMBL:AL021411) SC7H1.08C (202 aa), fasta scores; opt: 243 z-score: 810.6 E(): 0, 43.8% identity in 210 aa overlap 4 3 18 4 [Reporter]SCP1.109 (18P22)_PCR/5921 [Gene]SCP1.109/10787 NC_003888 SCP1.109, unknown, len: 63aa; 1 3 6 17 [Reporter]SCO7344 (6A2)_PCR/4158 [Gene]SCO7344/7567 NC_003888 SC4G10.23c, possible secreted protein, len: 258 aa. Weakly similar to the Eukaryotic protein elicitor from Pythium aphanidermatum TR:Q9SPD4(EMBL:AF179598) 25 kda protein elicitor (234 aa), fasta scores opt: 279 z-score: 316.6 E(): 3.6e-10 31.0% identity in 242 aa overlap. Contains a possible N-terminal signal sequence 4 3 18 3 [Reporter]SCO4003 (19L22)_PCR/5920 [Gene]SCO4003/10785 NC_003888 2SC10A7.07c, unknown, len: 117 aa 1 3 6 16 [Reporter]SCO4837 (6M22)_PCR/4157 [Gene]SCO4837/7565 NC_003888 SC5G8.05, glyA1, serine hydroxymethyltransferase, len: 481 aa; strongly similar to many e.g. SW:P34895 (GLYA_HYPME) serine hydroxymethyltransferase from Hyphomicrobium methylovorum (434 aa) fasta scores; opt: 912, z-score: 1044.8, E(): 0, 44.2% identity in 459 aa overlap. Contains two Pfam matches to entry PF00464 SHMT, Serine hydroxymethyltransferase. 4 3 18 2 [Reporter]SCO5985 (20D22)_PCR/5919 [Gene]SCO5985/10783 NC_003888 SCBAC16H6.20, conserved hypothetical protein, len: 132aa: similar to many eg. TR:BAB51882 (EMBL:AP003006) MLL5429 protein from Rhizobium loti (137 aa) fasta scores; opt: 271, Z-score: 331.4, 34.884% identity (35.156% ungapped) in 129 aa overlap. Contains Pfam match to entry PF01042 UPF0076, YjgF family. 1 3 6 15 [Reporter]SCO6960 (7I22)_PCR/4156 [Gene]SCO6960/7563 NC_003888 SC6F7.13c, unknown, len: 178 aa. 4 3 17 22 [Reporter]SCO1113 (1D18)_PCR/5917 [Gene]SCO1113/10781 NC_003888 2SCG38.06, conserved hypothetical protein, len: 384 aa; similar to TR:AAG04745 (EMBL:AE004565) Pseudomonas aeruginosa hypothetical 41.0 kDa protein PA1356, 368 aa; fasta scores: opt: 714 z-score: 833.2 E(): 0; 36.9% identity in 374 aa overlap. Contains 6x Pfam matches to entry PF02012 BNR, BNR repeat 1 3 6 14 [Reporter]SCO5934 (8E22)_PCR/4155 [Gene]SCO5934/7561 NC_003888 SC7H1.04, possible sigma factor, len: 200 aa; some similarity to sigma factors (ECF subfamily) from diverse organisms e.g. Alcaligenes eutrophus CNRH_ALCEU P37978 rna polymerase sigma factor cnrh (191 aa), fasta scores; opt: 230 z-score: 337.3 E(): 1.4e-11, 29.5% identity in 183 aa overlap 1 3 6 13 [Reporter]SCO6055 (9A22)_PCR/4154 [Gene]SCO6055/7559 NC_003888 SC9B1.02c, probable carbonic anhydrase, len: 193aa; similar to many eg. SW:CYNT_ECOLI carbonic anhydrase from Escherichia coli (219 aa) fasta scores; opt: 512, z-score: 618.8, E(): 3.8e-27, (43.6% identity in 188 aa overlap). Contains two Pfam matches to entry PF00484 Pro_CA, Prokaryotic-type carbonic anhydrases and Prosite matches to PS00705 Prokaryotic-type carbonic anhydrases signature 2 and PS00704 Prokaryotic-type carbonic anhydrases signature 1. 4 3 17 20 [Reporter]SCO6807 (3L14)_PCR/5915 [Gene]SCO6807/10777 NC_003888 SC1A2.16c, unknown, len: 597 aa. 1 3 6 12 [Reporter]SCO1961 (10M18)_PCR/4153 [Gene]SCO1961/7557 NC_003888 SCC54.21c, aroQ, dehydroquinate dehydratase, len: 157aa; identical to (except for disagreement on start codon, M or V) TR:O33608 (EMBL:AJ001493) aroQ, dehydroquinate dehydratase from Streptomyces coelicolor (157 aa) fasta scores; opt: 1049, z-score: 1325.4, E(): 0, (99.4% identity in 157 aa overlap). Contains PS01029 Dehydroquinase class II signature and Pfam match to entry PF01220 DHquinase_II, Dehydroquinase class II , score 234.80, E-value 1.2e-66. 4 3 17 19 [Reporter]SCO4816 (4H14)_PCR/5914 [Gene]SCO4816/10775 NC_003888 SC2A6.01c, hypothetical protein (fragment), len: >232 aa; similar to TR:O34320 (EMBL:AJ222587) Bacillus subtilis YkuG protein, 760 aa; fasta scores: opt: 439 z-score: 541.2 E(): 1.2e-22; 37.1% identity in 229 aa overlap,SCD63A.27c, hypothetical protein (fragment), len: >605 aa; similar to TR:O34320 (EMBL:AJ222587) Bacillus subtilis YkuG protein, 760 aa; fasta scores: opt: 961 z-score: 1162.8 E(): 0; 34.4% identity in 596 aa overlap 1 3 6 11 [Reporter]SCO4529 (11I18)_PCR/4152 [Gene]SCO4529/7555 NC_003888 SCD35.36, possible integral membrane protein, len: 117 aa. Contains possible hydrophobic membrane spanning regions 4 3 17 18 [Reporter]SCO2378 (5D14)_PCR/5913 [Gene]SCO2378/10773 NC_003888 SC4A7.06c, possible merR-family transciptional regulator, len: 196 aa; similar to SW:TIPA_STRLI (EMBL:S64314) Streptomyces lividans transcriptional activator TipA, 253 aa; fasta scores: opt: 187 z-score: 225.2 E(): 3.7e-05; 32.7% identity in 110 aa overlap. Contains Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family and a possible helix-turn-helix motif at residues 72..93 (+3.19 SD) 1 3 6 10 [Reporter]SCO4287 (12E18)_PCR/4151 [Gene]SCO4287/7553 NC_003888 SCD95A.20, hypothetical protein, len: 84 aa; similar to TR:P96276 (EMBL:Z84724) Mycobacterium tuberculosis hypothetical 11.7 kD protein MTCY22G10.27, 102 aa; fasta scores: opt: 244 z-score: 340.2 E(): 1.7e-11; 54.2% identity in 72 aa overlap 4 3 17 17 [Reporter]SCO6525 (6P10)_PCR/5912 [Gene]SCO6525/10771 NC_003888 SC5C7.10c, unknown, len: 255 aa; similar to hypothetical proteins from many organisms e.g. M. tuberculosis YZ34_MYCTU MTCY31.34 (372 aa), fasta scores; opt: 221 z-score: 293.2 E(): 4.4e-09, 30.7% identity in 251 aa overlap 1 3 6 9 [Reporter]SCO0153 (13A18)_PCR/4150 [Gene]SCO0153/7551 NC_003888 SCJ1.02c, probable secreted protein, len: 195 aa, similar to Actinosynnema pretiosum auranticum hypothetical 20.0 KD protein TR:O69206 (EMBL:U33059) (190 aa), fasta scores, opt: 524 z-score: 529.6 E(): 3.9e-22; 43.2% identity in 192 aa overlap. Contains a possible N-terminal signal peptide sequence 4 1 11 13 [Reporter]SCO5129 (10H4)_PCR/1744 [Gene]SCO5129/3199 NC_003888 SC9E12.14, possible ABC transporter ATP-binding protein, len: 318 aa; similar to TR:CAB88183 (EMBL:AL352972) Streptomyces coelicolor putative ABC transporter ATP-binding protein SCC30.13, 308 aa; fasta scores: opt: 861 z-score: 926.3 E(): 0; 54.4% identity in 309 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 1 3 6 8 [Reporter]SCO0859 (14M14)_PCR/4149 [Gene]SCO0859/7549 NC_003888 SCM2.12c, possible membrane protein, len: 169 aa. . Contains possible hydrophobic membrane spanning region 4 3 17 15 [Reporter]SCO5306 (8H10)_PCR/5910 [Gene]SCO5306/10767 NC_003888 SC6G9.27, possible protein phosphatase, len: 159 aa; very weak similarity to eukaryotic tyrosine and dual-specificity protein phosphatases e.g. TR:O70275 (EMBL:AF035645) Mus musculus protein tyrosine phosphatase 4A3 (173 aa), fasta scores; opt: 130 z-score: 173.4 E(): 0.025, 33.3% identity in 78 aa overlap. Alternative start codons are present at codons 12 and 17. Contains Pfam match to entry PF00782 DSPc, Dual specificity phosphatase, catalytic domain 1 3 6 7 [Reporter]SCO3718 (15I14)_PCR/4148 [Gene]SCO3718/7547 NC_003888 SCH35.06, probable cation transport system component, len: 554 aa; similar to many e.g. SW:ATKA_ECOLI AtkA/KdpA potassium transporting ATPase A chain from Escherichia coli (557 aa) fasta scores; opt: 1619, z-score: 1774.2, E(): 0, (48.3% identity in 555 aa overlap). Contains possible membrane spanning hydrophobic regions 4 3 17 14 [Reporter]SCO5504 (9D10)_PCR/5909 [Gene]SCO5504/10765 NC_003888 SC8D9.16, possible integral membrane protein, len: 208aa; alanine-rich, contains possible membrane spanning regions. 1 3 6 6 [Reporter]SCO0201 (16E14)_PCR/4147 [Gene]SCO0201/7545 NC_003888 SCJ12.13c, possible integral membrane protein, len: 223 aa. Contains potential membrane spanning hydrophobic domains. 4 3 17 13 [Reporter]SCO2503 (10P6)_PCR/5908 [Gene]SCO2503/10763 NC_003888 SCC121.06, probable chitinase precursor, len: 358 aa; similar to C-terminal region of SW:CHID_BACCI (EMBL:D10594) Bacillus circulans chitinase D precursor (EC 3.2.1.14) ChiD, 497 aa; fasta scores: opt: 471 z-score: 502.4 E(): 1.4e-20; 33.0% identity in 370 aa overlap and to SW:CHIX_STROI (EMBL:X71080) Streptomyces olivaceoviridis (Streptomyces corchorusii exochitinase 1 precursor (EC 3.2.1.14) Chi01, 597 aa; fasta scores: opt: 249 z-score: 267.3 E(): 1.7e-07; 31.2% identity in 391 aa overlap. Contains Pfam match to entry PF00704 Glyco_hydro_18 and match to Prosite enry PS01095 Chitinases family 18 active site. Contains also possible N-terminal region signal peptide sequence 1 3 6 5 [Reporter]SCO3863 (17A14)_PCR/4146 [Gene]SCO3863/7543 NC_003888 SCH69.33, putative secreted protein, len: 129 aa; similar to TR:CAA09663 (EMBL:AJ011500) hypothetical protein from the granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22 (124 aa) fasta scores; opt: 259, z-score: 293.5, E(): 5.1e-09, (41.5% identity in 118 aa overlap). Contains possible N-terminal region signal peptide sequence 4 3 17 12 [Reporter]SCO4379 (11L6)_PCR/5907 [Gene]SCO4379/10761 NC_003888 SCD10.11, conserved hypothetical protein, len: 558 aa; similar to TR:O86320 (EMBL:Z79700) Mycobacterium tuberculosis hypothetical 59.1 kD protein aa; fasta scores: opt: 2549 z-score: 2481.8 E(): 0; 69.0% identity in 562 aa overlap 1 3 6 4 [Reporter]SCO1737 (18M10)_PCR/4145 [Gene]SCO1737/7541 NC_003888 SCI11.26, hypothetical protein, len: 124 aa; unknown function, similar to TR:O86372 (EMBL:AL022003) Mycobacterium tuberculosis hypothetical protein (116 aa), fasta scores; opt: 129 z-score: 177.0 E(): 0.016, 34.7% identity in 98 aa overlap 4 1 11 7 [Reporter]SCO3915 (15P20)_PCR/1738 [Gene]SCO3915/3189 NC_003888 SCH24.37, probable transmembrane efflux protein, len: 404 aa; similar to many e.g. TR:Q56147 (EMBL:U09991), cmlV, Streptomyces violaceus chloramphenicol efflux pump (436 aa), fasta scores; opt: 1012 z-score: 1048.2 E(): 0, 40.5% identity in 395 aa overlap. Similar to other putative efflux proteins from S.coelicolor e.g. SCE94.17c (EMBL:AL049628) possible exporter (531 aa) (26.8% identity in 395 aa overlap). Contains several hydrophobic, probable membrane-spanning regions. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter, score -61.60, E-value 0.00088 1 3 6 3 [Reporter]SCP1.105 (19I10)_PCR/4144 [Gene]SCP1.105/7539 NC_003888 SCP1.105, possible integral membrane plasmid transfer protein, len: 153aa; similar to TR:Q57241 (EMBL:U23762) SpbB3 protein (involved in intramycelial plasmid spread) from plasmid pJV1 of Streptomyces phaeochromogenes (147 aa) fasta scores; opt: 317, z-score: 394.0, E(): 1.8e-14, 46.5% identity in 142 aa overlap. 4 3 17 10 [Reporter]SCO3072 (13D6)_PCR/5905 [Gene]SCO3072/10757 NC_003888 SCE25.13c, probable amino acid hydrolase, len: 400 aa; similar to SW:AMB2_BACST (EMBL:S67784) Bacillus stearothermophilus N-carbamyl-L-amino acid amidohydrolase (EC 3.5.1.-) AmaB, 409 aa; fasta scores: opt: 733 z-score: 804.8 E(): 0; 34.7% identity in 404 aa overlap 1 3 6 2 [Reporter]SCP1.278 (20A10)_PCR/4143 [Gene]SCP1.278/7537 NC_003888 SCP1.278, unknown, len: 361aa; 4 3 17 9 [Reporter]SCO0393 (14P2)_PCR/5904 [Gene]SCO0393/10755 NC_003888 SCF62.19, possible transferase, len: 269 aa. Similar to many transferases e.g. Streptomyces glaucescens TR:Q54266 (EMBL:X82177) alpha-D-glucose-1-phosphate cytidylyl-transferase StrQ (fragment) (299 aa), fasta scores opt: 554 z-score: 687.3 E(): 6.3e-31 35.0% identity in 280 aa overlap. 1 3 5 22 [Reporter]SCO5385 (1A6)_PCR/4141 [Gene]SCO5385/7535 NC_003888 2SC6G5.29, possible 3-hydroxybutyryl-coA dehydrogenase, len: 282 aa; similar to many eg. SW:P52041 (HBD_CLOAB) 3-hydroxybutyryl-coA dehydrogenase from Clostridium acetobutylicum (282 aa) fasta scores; opt: 806, z-score: 947.0, E(): 0, 44.6% identity in 280 aa overlap. Contains Pfam match to entry PF00725 3HCDH, 3-hydroxyacyl-CoA dehydrogenase and Prosite match to PS00067 3-hydroxyacyl-CoA dehydrogenase signature. 4 3 17 8 [Reporter]SCO3759 (15L2)_PCR/5903 [Gene]SCO3759/10753 NC_003888 SCH63.06c, possible integral membrane protein, len: 403 aa; similar to TR:Q9X8Q9 (EMBL:AL049754) Streptomyces coelicolor putative membrane protein SCH10.20c, 420 aa; fasta scores: opt: 898 z-score: 1025.8 E(): 0; 45.3% identity in 395 aa overlap. Contains possible hydrophobic membrane spanning regions 1 3 5 21 [Reporter]SCO2291 (2M2)_PCR/4140 [Gene]SCO2291/7533 NC_003888 SCC75A.37, axeA, secreted acetylxylan esterase, len: 335 aa. Almost identical in sequence with Streptomyces lividans TR:Q54413 (EMBL:M64552) acetylxylan esterase precursor (EC 3.1.1.72) (335 aa), fasta scores opt: 2197 z-score: 2058.4 E(): 0 98.5% identity in 335 aa overlap. Also highly similar to the C-terminus of Cellulomonas fimi SW:XYND_CELFI (EMBL:X76729) endo-1,4-beta-xylanase D precursor (EC 3.2.1.8) (644 aa), fasta scores opt: 1108 z-score: 1040.5 E(): 0 50.0% identity in 346 aa overlap. Also similar to the adjoining CDS on this cosmid, xlnB, (335 aa), fasta scores opt: 652 z-score: 554.2 E(): 1.7e-25 56.2% identity in 201 aa overlap. Contains a possible N-terminal signal sequence and a Pfam match to entry PF01522 Polysac_deacet, Polysaccharide deacetylase 4 3 17 7 [Reporter]SCO0762 (16H2)_PCR/5902 [Gene]SCO0762/10751 NC_003888 SCF81.21c, sti1, protease inhibitor precursor, len: 144 aa; identical to SW:SSI_STRLI (EMBL:M80576) Streptomyces lividans protease inhibitor precursor (SlpI) (trypsin inhibitor) Sti1, 144 aa and highly similar to SW:SSI2_STRLO (EMBL:M80577) Streptomyces longisporus trypsin inhibitor Sti2 precursor, 144 aa; fasta scores: opt: 735 z-score: 837.0 E(): 0; 76.4% identity in 144 aa overlap. Contains match to Pfam entry PF00720 SSI, Subtilisin inhibitor-like and match to Prosite entry PS00999 Streptomyces subtilisin-type inhibitors signature 1 3 5 20 [Reporter]SCO2688 (3I2)_PCR/4139 [Gene]SCO2688/7531 NC_003888 SCC61A.09, ribD, riboflavin-specific deaminase, len: 381aa; similar to many eg. SW:P25539 (RIBD_ECOLI) ribD, riboflavin-specific deaminase from Escherichia coli (367 aa) fasta scores; opt: 581, z-score: 642.9, E(): 2.4e-28, 36.3% identity in 386 aa overlap. Contains Pfam match to entry PF01872 RibD_C, RibD C-terminal domain, Pfam match to entry PF00383 dCMP_cyt_deam, Cytidine and deoxycytidylate deaminase zinc-binding region and PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. 4 1 11 2 [Reporter]SCO3993 (20H16)_PCR/1733 [Gene]SCO3993/3179 NC_003888 SCBAC25E3.30c, unknown, doubtful CDS, len: 89 aa: no significant database matches. 1 3 5 19 [Reporter]SCO6896 (4E2)_PCR/4138 [Gene]SCO6896/7529 NC_003888 SC1B2.02, possible integral membrane protein, len: 411 aa. Weakly similar to several e.g. Escherichia coli TR:Q47390(EMBL:D16251) erythromycin resistance determinant, MrX (388 aa), fasta scores opt: 263 z-score: 283.2 E(): 2.6e-08 26.5% identity in 389 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 4 3 17 5 [Reporter]SCO0856 (17P22)_PCR/5900 [Gene]SCO0856/10747 NC_003888 SCM2.09c possible integral membrane protein, len: 408 aa. Contains multiple possible membrane spanning hydrophobic domains. Also contains a TTA /leucine codon, which is a possible target for bldA regulation. 1 3 5 18 [Reporter]SCO6334 (5A2)_PCR/4137 [Gene]SCO6334/7527 NC_003888 SC3A7.02c, probable transcritional regulator, len: 892 aa; similar to e.g. MALT_ECOLI MalT regulatory protein (901 aa), fasta scores; opt: 540 z-score: 495.9 E(): 2.3e-20, 26.2% identity in 936 aa overlap. Contains PS00017 ATP /GTP-binding site motif A (P-loop), helix-turn-helix motif from aa 848 to 869 (Score 1229, +3.37 SD) and Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 50.40, E-value 3.8e-11 4 3 17 3 [Reporter]SCO4992 (19H22)_PCR/5898 [Gene]SCO4992/10745 NC_003888 2SCK36.15, unknown, len: 214 aa. High content of alanine and glutamic amino acid residues in C-terminal region 1 3 5 17 [Reporter]SCO7060 (5M22)_PCR/4136 [Gene]SCO7060/7525 NC_003888 SC4G1.26c, conserved hypothetical protein, len: 201 aa; similar to TR:Q9S2N1 (EMBL:AL109661) Streptomyces coelicolor hypothetical 24.3 kDa protein SC6E10.06c, 215 aa; fasta scores: opt: 540 z-score: 638.3 E(): 4.4e-28; 42.9% identity in 198 aa overlap 4 3 17 2 [Reporter]SCO5980 (20P18)_PCR/5897 [Gene]SCO5980/10743 NC_003888 SCBAC16H6.15, possible bifunctional hydroxylase/oxidoreductase, len: 801aa: N-terminal region similar to hydroxylases eg. TR:Q9S3U8 (EMBL:AF172851) hydroxylase VioD from Chromobacterium violaceum (373 aa) fasta scores; opt: 549, Z-score: 591.4, 31.768% identity (33.430% ungapped) in 362 aa overlap and TR:Q9X7R5 (EMBL:AL049863) putative hydroxylase SC5H1.07c from Streptomyces coelicolor (421 aa) fasta scores; opt: 957, Z-score: 1027.4, 44.473% identity (47.527% ungapped) in 389 aa overlap. C-terminal region similar to oxidoreductases eg. TR:Q9R9V9 (EMBL:AF154061) xenobiotic reductase XenA from Pseudomonas putida (363 aa) fasta scores; opt: 895, Z-score: 962.0, 39.831% identity (40.517% ungapped) in 354 aa overlap and TR:Q9EX17 (EMBL:AL445503) putative oxidoreductase from Streptomyces coelicolor (359 aa) fasta scores; opt: 1008, Z-score: 1083.0, 46.667% identity (48.276% ungapped) in 360 aa overlap. Contains Pfam match to entry PF01360 Monooxygenase, Monooxygenase; Pfam match to entry PF00724 oxidored_FMN, NADH:flavin oxidoreductase / NADH oxidase family; Prosite match to PS00133 Zinc carboxypeptidases, zinc-binding region 2 signature and Prosite match to PS00383 Tyrosine specific protein phosphatases active site. 1 3 5 16 [Reporter]SCO7320 (6I22)_PCR/4135 [Gene]SCO7320/7523 NC_003888 SC5F8.30c, unknown, len: 580 aa. Similar in parts to many Streptomyces coelicolor proteins of undefined function including: TR:Q9RJE1(EMBL:AL133171) putative regulatory protein, SCF81.10C (828 aa), fasta scores opt: 676 z-score: 710.0 E(): 4.5e-32 34.7% identity in 426 aa overlap and TR:Q9X8W1(EMBL:AL078610;) hypothetical 66.2 kd protein, SCH35.45C (634 aa), fasta scores opt: 633 z-score: 666.6 E(): 1.2e-29 33.4% identity in 539 aa overlap. 4 3 16 22 [Reporter]SCO1213 (1P14)_PCR/5895 [Gene]SCO1213/10741 NC_003888 2SCG58.13, hypotetical protein, len: 242 aa; similar to TR:Q9ZGG8 (EMBL:AF080002) Heliobacillus mobilis cobyric acid synthase CobQ, 252 aa; fasta scores: opt: 505 z-score: 574.8 E(): 1.5e-24; 39.5% identity in 238 aa overlap 1 3 5 15 [Reporter]SCO7430 (7E22)_PCR/4134 [Gene]SCO7430/7521 NC_003888 SC6D11.26, unknown, len: 228 aa. Contains a Pfam match to entry PF01955 DUF105, Domain of unknown function. 4 1 10 18 [Reporter]SCO6389 (5H8)_PCR/1727 [Gene]SCO6389/3169 NC_003888 SC3C8.08c, unknown, len: 130 aa 1 3 5 14 [Reporter]SCO6893 (8A22)_PCR/4133 [Gene]SCO6893/7519 NC_003888 SC7F9.45, unknown, len: 103 aa. 4 3 16 20 [Reporter]SCO2224 (3H14)_PCR/5893 [Gene]SCO2224/10737 NC_003888 SC10B7.19, unknown, len: 154 aa. High content in alanine and proline amino acid residues. Also high G+C content (80.3%) 1 3 5 13 [Reporter]SCO6440 (9M18)_PCR/4132 [Gene]SCO6440/7517 NC_003888 SC9B5.07, conserved hypothetical protein, len: 250aa; similar to 3 eg. SW:YA99_METJA hypothetical protein MJ1099 from Methanococcus jannaschii (235 aa) fasta scores;opt: 848, z-score: 1229.1, E(): 0, (57.9% identity in 233 aa overlap). 4 3 16 19 [Reporter]SCO5483 (4D14)_PCR/5892 [Gene]SCO5483/10735 NC_003888 SC2A11.17c, probable transcriptional regulator, len: 208 aa; similar to members of the tetR family e.g. TCMR_STRGA tetracenomycin C transcriptional repressor (226 aa), fasta scores; opt: 138 z-score: 224.5 E(): 3e-05, 26.5% identity in 155 aa overlap. Contains helix-turn-helix motif at aa 36-57 (Score 1332, +3.72 SD) and Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family, score 23.40, E-value 9.9e-05 1 3 5 12 [Reporter]SCO1957 (10I18)_PCR/4131 [Gene]SCO1957/7515 NC_003888 SCC54.17, probable fructokinase, len: 302 aa; similar to many e.g. SW:SCRK_RHILT Frk, fructokinase from Rhizobium leguminosarum biovar trifolii (326 aa) fasta scores; opt: 617, z-score: 682.4, E(): 1.1e-30, (41.0% identity in 288 aa overlap). Contains PS00584 pfkB family of carbohydrate kinases signature 2 and Pfam match to entry PF00294 pfkB, pfkB family carbohydrate kinase, score 132.90, E-value 9.6e-41 4 3 16 18 [Reporter]SCO2097 (5P10)_PCR/5891 [Gene]SCO2097/10733 NC_003888 SC4A10.30c, possible membrane protein, len: 135 aa; unknown function, similar to mycobacterial hypothetical proteins TR:O53503 (EMBL:AL021957) Mycobacterium tuberculosis (134 aa), fasta scores; opt: 349 z-score: 414.6 E(): 9e-16, 44.1% identity in 136 aa overlap and its homologue in Mycobacterium leprae TR:O69562 (EMBL:AL022602) Mycobacterium leprae hypothetical protein (134 aa) (42.6% identity in 136 aa overlap). Contains hydrophobic, possible membrane-spanning regions 1 3 5 11 [Reporter]SCO4366 (11E18)_PCR/4130 [Gene]SCO4366/7513 NC_003888 SCD19.21, possible phosphoserine aminotransferase, len: 363 aa; similar to SW:SERC_MYCTU (EMBL:Z73101) Mycobacterium tuberculosis putative phosphoserine aminotransferase (EC 2.6.1.52) Serc or MTCY31.12c, 376 aa; fasta scores: opt: 1693 z-score: 1994.6 E(): 0; 66.9% identity in 363 aa overlap 4 3 16 17 [Reporter]SCO6521 (6L10)_PCR/5890 [Gene]SCO6521/10731 NC_003888 SC5C7.06, probable secreted protein, len: 318 aa; contains N-terminal signal sequence 1 3 5 9 [Reporter]SCO3166 (13M14)_PCR/4128 [Gene]SCO3166/7511 NC_003888 SCE87.17c, possible membrane transport protein, len: 745 aa. Highly similar to several other Streptomyces coelicolor putative transport proteins: TR:CAB56653 (EMBL:AL121596) putative membrane transport protein SCF51A.01C (765 aa), fasta scores opt: 1664 z-score: 1670.0 E(): 0 57.8% identity in 760 aa overlap and TR:Q9Z577(EMBL:AL035569) putative integral membrane export protein SC8D9.13 (748 aa), fasta scores opt: 1382 z-score: 1387.5 E():0 40.7% identity in 734 aa overlap. Also similar to a number of other putative MMPL family membrane proteins such as: Mycobacterium leprae SW:MMLB_MYCLE(EMBL:Z95398) putative membrane protein MMPL11 (1014 aa), fasta scores opt: 999 z-score: 1001.8 E(): 0 29.6% identity in 743 aa overlap. Contains multiple possible membrane spanning hydrophobic domains and a possible N-terminal signal sequence. 4 1 10 13 [Reporter]SCO5125 (10D4)_PCR/1722 [Gene]SCO5125/3159 NC_003888 SC9E12.10c, hypothetical protein, len: 287 aa; similar to TR:Q9RDR4 (EMBL:AL136500) Streptomyces coelicolor hypothetical 31.7 kDa protein SC1G2.15c, 284 aa; fasta scores: opt: 1240 z-score: 1466.1 E(): 0; 67.2% identity in 287 aa overlap 1 3 5 8 [Reporter]SCO3901 (14I14)_PCR/4127 [Gene]SCO3901/7509 NC_003888 SCH24.23, probable secreted penicillin-binding protein, len: 795 aa; similar to many e.g. SW:PBPF_BACSU (EMBL:Y14083), PbpF, Bacillus subtilis penicillin-binding protein 1A (714 aa), fasta scores; opt: 439 z-score: 369.7 E(): 2.8e-13, 31.2% identity in 683 aa overlap. Contains hydrophobic, possible membrane-spanning region at N-terminus. Contains Pfam matches to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain, score 49.60, E-value 2.8e-11 and entry PF00912 Transglycosyl, Transglycosylase, score 172.40, E-value 7.3e-48. Contains PS00017 ATP/GTP-binding site motif A (P-loop) 4 3 16 15 [Reporter]SCO5310 (8D10)_PCR/5888 [Gene]SCO5310/10727 NC_003888 SC6G9.23, hypothetical protein, len: 187 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference, amino acid composition and the presence of a possible RBS 1 3 5 7 [Reporter]SCO3864 (15E14)_PCR/4126 [Gene]SCO3864/7507 NC_003888 SCH18.01, probable transcriptional regulator (fragment), len: >70 aa; similar to TR:Q9X8D7 (EMBL:AL049573) Streptomyces coelicolor putative GntR-family transcriptional regulator SCE39.14c, 243 aa; fasta scores: opt: 142 z-score: 189.8 E(): 0.0042; 36.8% identity in 57 aa overlap. Overlaps with Streptomyces coelicolor putative transcriptional regulator (fragment) SCH69.34, 225 aa,SCH69.34, probable transcriptional regulator, len: >225aa; similar to many egs. TR:Q07191 (EMBL:Z19589) KorSA regulatory protein from the pSAM2 plasmid of Streptomyces ambofaciens (259 aa) fasta scores; opt: 224, z-score: 275.8, E(): 4.9e-08, (34.3% identity in 233 aa overlap) and SW:HUTC_KLEAE repressor for the histidine utilization genes of Klebsiella aerogenes (241 aa) fasta scores; opt: 180, z-score: 223.7, E(): 3.9e-05, (30.4% identity in 214 aa overlap). Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and probable helix-turn-helix 29-50aa (+3.13 SD). 4 3 16 14 [Reporter]SCO7840 (9P6)_PCR/5887 [Gene]SCO7840/10725 NC_003888 SC8E7.37, unknown, len: 148 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1Y6 (EMBL:AL109973) hypothetical protein, SCJ30.02c (148 aa). 1 3 5 6 [Reporter]SCO0639 (16A14)_PCR/4125 [Gene]SCO0639/7505 NC_003888 SCF56.23c, probable integral membrane protein, len: 310 aa; similar to TR:O86628 (EMBL:AL031155) Streptomyces coelicolor putative integral membrane protein SC3A7.27, 309 aa; fasta scores: opt: 577 z-score: 631.4 E(): 8.7e-28; 41.9% identity in 298 aa overlap 4 3 16 13 [Reporter]SCO2499 (10L6)_PCR/5886 [Gene]SCO2499/10723 NC_003888 SCC121.02c, possible transport ATPase, len: 1472 aa; similar to TR:P96271 (EMBL:Z84724) Mycobacterium tuberculosis hypothetical 159.8 kD protein MTCY22G10.22c, 1539 aa; fasta scores: opt: 2216 z-score: 2157.1 E(): 0; 38.4% identity in 1543 aa overlap and N-terminal region similar to SW:ATMA_SALTY (EMBL:U07843) Salmonella typhimurium Mg(2+) transport atpase, P-type 1 (EC 3.6.1.-) MgtA, 902 aa; fasta scores: opt: 800 z-score: 779.8 E(): 0 ;28.0% identity in 880 aa overlap. Contains Pfam match to entry PF00122 E1-E2_ATPase, match to Prosite entry PS00154 E1-E2 ATPases phosphorylation site and possible N-terminal region signal peptide sequence 1 3 5 5 [Reporter]SCO3840 (17M10)_PCR/4124 [Gene]SCO3840/7503 NC_003888 SCH69.10c, unknown, len: 271aa; similar to TR:P74322 (EMBL:D90914) from Synechocystis sp. PCC6803 (228 aa) fasta scores; opt: 238, z-score: 277.0, E(): 4.2e-08, (26.6% identity in 259 aa overlap). Contains possible coiled-coil at 30-40aa. 4 3 16 12 [Reporter]SCO2290 (11H6)_PCR/5885 [Gene]SCO2290/10721 NC_003888 SCC75A.36, unknown, len: 113 aa. Weakly similar to Bacillus subtilis SW:YPJD_BACSU(EMBL:L38424) hypothetical 13.0 KD protein (111 aa), fasta scores opt:103 z-score: 145.9 E():0.94 30.8% identity in 91 aa overlap. 1 3 5 4 [Reporter]SCO0283 (18I10)_PCR/4123 [Gene]SCO0283/7501 NC_003888 SCF85.11c, unknown, len: 164 aa. 4 1 10 8 [Reporter]SCO1795 (14P20)_PCR/1717 [Gene]SCO1795/3149 NC_003888 SCI5.03, unknown, len: 168 aa; similar to TR:P72833 (EMBL:D90901) hypothetical protein from Synechocystis sp. PCC6803 (165 aa) fasta scores; opt: 417, z-score: 517.5, E(): 1.7e-21, (46.9% identity in 143 aa overlap) 1 3 5 3 [Reporter]SCO7511 (19E10)_PCR/4122 [Gene]SCO7511/7499 NC_003888 SCBAC25F8.03, gap2, glyceraldehyde 3-phosphate dehydrogenase, len: 332 aa; highly similar to SW:G3P_STRAU (EMBL:U21191) Streptomyces aureofaciens glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) Gap, 332 aa; fasta scores: opt: 1898 Z-score: 2070.6 bits: 391.5 E(): 1e-107; 88.855% identity in 332 aa overlap and to TR:CAB38137 (EMBL:AL035591) Streptomyces coelicolor glyceraldehyde 3-phosphate dehydrogenase Gap or SCC54.07c, 337 aa; fasta scores: opt: 1272 Z-score: 1174.4 bits: 225.7 E(): 1.1e-59; 57.831% identity in 332 aa overlap. Contains Pfam matches to entries PF00044 gpdh, Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain and PF02800 gpdh_C, Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain and match to Prosite entry PS00071 Glyceraldehyde 3-phosphate dehydrogenase active site 1 3 5 2 [Reporter]SCO1877 (20M6)_PCR/4121 [Gene]SCO1877/7497 NC_003888 SCI39.24, unknown, len: 224 aa 1 3 4 22 [Reporter]SCO4545 (1M2)_PCR/4119 [Gene]SCO4545/7495 NC_003888 2SCD4.16, hypothetical protein, len: 304 aa; identical to previously sequenced TR:Q9XD94 (EMBL:AF106004) Streptomyces coelicolor hypothetical 34.1 kDa protein, 304 aa and highly similar to TR:CAB81866 (EMBL:AL161691) Streptomyces coelicolor hypothetical protein SCD40A.21c, 302 aa; fasta scores: opt: 850 z-score: 894.4 E(): 0; 45.0% identity in 300 aa overlap 1 3 4 21 [Reporter]SCO5366 (2I2)_PCR/4118 [Gene]SCO5366/7493 NC_003888 2SC6G5.10, atpI, ATP synthase protein I, len: 144 aa; identical to SW:P50015 (ATPZ_STRLI) ATP synthase protein I from Streptomyces lividans (144 aa). Contains possible membrane-spanning hydrophobic regions 1 3 4 20 [Reporter]SCO1620 (3E2)_PCR/4117 [Gene]SCO1620/7491 NC_003888 SCI41.03c, opuABC glycine betaine transport system permease protein, len: 871 aa. N-terminal region highly similar to SW:OPUB_BACSU (P46921) Bacillus subtilis glycine betaine transport system permease protein ; fasta scores: opt: 847 z-score: 884.1 E(): 0; 47.2% identity in 269 aa overlap, and C-terminal region similar to SW:OPUC_BACSU glyine betaine-binding protein precusor; fasta scores; opt: 322 z-score: 339.7 E(): 1.5e-11; 32.8% identity in 271 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, binding-protein-dependent transport systems inner membrane component and prosite match to entry PS00402 Binding-protein-dependent transport systems inner membrane component sign 1 3 4 19 [Reporter]SCO6162 (4A2)_PCR/4116 [Gene]SCO6162/7489 NC_003888 SC1A9.26c, probable luxR family two-component system response regulator, len: 260aa; similar to a family of response regulators eg. TR:O69961 (EMBL:AL022268) probable two-component transcriptional regulator from Streptomyces coelicolor (219 aa) fasta scores; opt:1079, z-score: 1434.4, E():0, (77.5% identity in 218 aa overlap) and DEGU_BACBR (EMBL:L15444) transcriptional activator protein DegU from Bacillus brevis (236 aa) fasta scores; opt:378, z-score: 682.5, E(): 9.7e-31, (36.6% identity in 227 aa overlap). Contains PS00622 Bacterial regulatory proteins, luxR family signature, Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family, score 99.70, E-value 5.6e-26, Pfam match to entry PF00072 response_reg, Response regulator receiver domain, score 132.90, E-value 5.6e-36 and an helix-turn-helix motif from: 1 to: 260, Score 1221 (+3.35 SD). 1 3 4 18 [Reporter]SCO2025 (4M22)_PCR/4115 [Gene]SCO2025/7487 NC_003888 SC3A3.03c, gltD, probable glutamate synthase small subunit, len: 487 aa; similar to bacterial glutamate synthases e.g. TR:Q51584 (EMBL:D85230), gltD, Plectonema boryanum small subunit of NADH-dependent glutamate synthase (492 aa), fasta scores; opt: 1295 z-score: 1405.0 E(): 0, 51.2% identity in 500 aa overlap. Also similar to part of eukaryotic glutamate synthases e.g. SW:GLSN_MEDSA (EMBL:L01660) Medicago sativa (Alfalfa) Glutamate synthase [NADH] precursor (2194 aa) (49.1% identity in 489 aa overlap). Contains Pfam match to entry PF00037 fer4, 4Fe-4S ferredoxins and related iron-sulfur cluster binding domains 1 3 4 17 [Reporter]SCO7056 (5I22)_PCR/4114 [Gene]SCO7056/7485 NC_003888 SC4G1.22c, possible gntR-family transcriptional regulator, len: 253 aa; similar to TR:Q9RYU6 (EMBL:AE001863) Deinococcus radiodurans transcriptional regulator, gntR-family DRA0211, 279 aa; fasta scores: opt: 217 z-score: 236.0 E(): 1.1e-05; 37.6% identity in 141 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family and match to Prosite entry PS00043 Bacterial regulatory proteins, gntR family signature. Also contains a possible helix-turn-helix at residues 47..68 (+4.70 SD) 1 3 4 16 [Reporter]SCO7588 (6E22)_PCR/4113 [Gene]SCO7588/7483 NC_003888 SC5F1.42c, possible oxidoreductase, len: 306 aa; similar to various Streptomyces coelicolor putative oxidoreductases/dehydrogenases, e.g. TR:Q9RIU1 (EMBL:AL133278) putative dehydratase SCM11.21c, 319 aa; fasta scores: opt: 912 z-score: 984.0 E(): 0; 47.4% identity in 312 aa overlap 1 3 4 15 [Reporter]SCO7425 (7A22)_PCR/4112 [Gene]SCO7425/7481 NC_003888 SC6D11.21, possible multidrug-efflux transporter protein, len: 577 aa. Highly similar to several including: Streptomyces virginiae TR:Q9XE01(EMBL:AB019519) virginiamycin S resistance protein, VarS (518 aa), fasta scores opt: 322 z-score: 328.6 E(): 7e-11 25.7% identity in 536 aa overlap and Deinococcus radiodurans TR:AAF11851(EMBL:AE002062) multidrug-efflux transporter, putative (410 aa), fasta scores opt: 937 z-score: 945.6 E(): 0 41.3% identity in 395 aa overlap. Contains a Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and multiple possible membrane spanning hydrophobic domains. 1 3 4 14 [Reporter]SCO7593 (8M18)_PCR/4111 [Gene]SCO7593/7479 NC_003888 SC7H9.05, unknown, len: 121 aa 4 3 15 21 [Reporter]SCO3136 (2H14)_PCR/5872 [Gene]SCO3136/10697 NC_003888 SCE66.15c, galK, galactokinase (EC 2.7.1.6), len: 387aa; strongly similar to many eg. SW:P13227 (GAL1_STRLI) galactokinase from Streptomyces lividans (397 aa) fasta scores; opt: 2131, z-score: 2454.6, E(): 0, 88.8% identity in 383 aa overlap. Contains Pfam match to entry PF00288 GHMP_kinases, GHMP kinases putative ATP-binding proteins, Prosite match to PS00627 GHMP kinases putative ATP-binding domain and Prosite match to PS00106 Galactokinase signature. 1 3 4 13 [Reporter]SCO6436 (9I18)_PCR/4110 [Gene]SCO6436/7477 NC_003888 SC9B5.03, probable tRNA synthetase, len: 506 aa; similar to many eg. SW:SYM_METTH methionyl-tRNA synthetase from Methanobacterium thermoautotrophicum (651 aa) fasta scores; opt: 610, z-score: 560.9, E(): 5.9e-24, (28.9% identity in 402 aa overlap). Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. 4 3 15 20 [Reporter]SCO2220 (3D14)_PCR/5871 [Gene]SCO2220/10695 NC_003888 SC10B7.15, unknown, len: 643 aa 1 3 4 12 [Reporter]SCO2458 (10E18)_PCR/4109 [Gene]SCO2458/7475 NC_003888 SCC24.29c, hypothetical protein, len: 172 aa; similar to TR:Q9X8S3 (EMBL:AL049754) Streptomyces coelicolor hypothetical 20.1 kD protein SCH10.34c, 185 aa; fasta scores: opt: 412 z-score: 505.6 E(): 1e-20; 40.7% identity in 172 aa overlap 4 3 15 19 [Reporter]SCO7015 (4P10)_PCR/5870 [Gene]SCO7015/10693 NC_003888 SC1H10.04c, possible secreted glycosyl hydrolase, len: 1433 aa. Identical to Streptomyces lividans TR:P75020(EMBL:U22894) chitinase homolog found within the 4.7 kb amplifiable unit of the AUD1 element (1433 aa), fasta scores opt: 9322 z-score: 9526.2 E():0 99.5% identity in 1433 aa overlap. C-terminal similar to TR:BAB62749 (EMBL:AB055506) mycodextranase from Streptomyces sp. J-13-3 fasta scores; opt: 865, Z-score: 887.6, 32.953% identity (36.861% ungapped) in 613 aa overlap. Also weakly similar, in parts, to Bacillus circulans SW:CHI1_BACCI(EMBL:M57601) chitinase A1 precursor (EC 3.2.1.14) (699 aa), fasta scores opt: 497 z-score: 507.9 E(): 7.2e-21 47.3% identity in 182 aa overlap. Contains a possible N-terminal signal sequence and 2x Pfam matches to entries PF00041 fn3, Fibronectin type III domain. 1 3 4 11 [Reporter]SCO4362 (11A18)_PCR/4108 [Gene]SCO4362/7473 NC_003888 SCD19.17, possible two component system sensor kinase, len: 403 aa; similar to TR:O70000 (EMBL:AL022374) Streptomyces coelicolor putative two-component sensor SC5B8.14, 384 aa; fasta scores: opt: 580 z-score: 600.9 E(): 5.7e-26; 36.8% identity in 353 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 15 18 [Reporter]SCO2093 (5L10)_PCR/5869 [Gene]SCO2093/10691 NC_003888 SC4A10.26, conserved hypothetical protein, len: 167 aa; unknown function, similar to other hypothetical proteins e.g. SW:Y023_MYCTU (EMBL:Z73419) Mycobacterium tuberculosis hypothetical protein (163 aa), fasta scores; opt: 567 z-score: 691.9 E(): 3.2e-31, 53.4% identity in 163 aa overlap. Very weak similarity to the C-terminus of many carbonic anhydrases e.g. TR:P93109 (EMBL:U80805) Chlamydomonas reinhardtii beta-carbonic anhydrase (267 aa) (24.3% identity in 181 aa overlap) 1 3 4 10 [Reporter]SCO3289 (12M14)_PCR/4107 [Gene]SCO3289/7471 NC_003888 SCE15.06, possible large membrane protein, len: 536 aa; contains a possible membrane spanning hydrophobic region 1 3 4 9 [Reporter]SCO3691 (13I14)_PCR/4106 [Gene]SCO3691/7469 NC_003888 SCH35.33c, possible regulatory protein, len: 422 aa; regions of similarity to many egs. TR:O86807 (EMBL:AL031031) putative regulatory protein from Streptomyces coelicolor (916 aa) fasta scores; opt: 541, z-score: 629.7, E(): 9.6e-28, (35.5% identity in 349 aa overlap) SW:RSBU_BACSU sigma factor associated regulatory protein from Bacillus subtilis (335 aa) fasta scores; opt: 214, z-score: 253.9, E(): 8.2e-07, (26.8% identity in 228 aa overlap). Contains probable coiled-coil region at approx. 150-165aa. 4 3 15 16 [Reporter]SCO5687 (7D10)_PCR/5867 [Gene]SCO5687/10687 NC_003888 SC5H4.11, probable transport system integral membrane protein, len: 346 aa; similar to SW:LPLB_BACSU (EMBL:L19163) Bacillus subtilis LplB protein, 318 aa; fasta scores: opt: 814 z-score: 950.7 E(): 0; 38.2% identity in 309 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 1 3 4 8 [Reporter]SCO1538 (14E14)_PCR/4105 [Gene]SCO1538/7467 NC_003888 SCL2.28c, probable transport system membrane protein, len: 328 aa; similar to SW:YESP_BACSU (EMBL:Z99107) Bacillus subtilis probable ABC transporter permease protein YesP, 309 aa; fasta scores: opt: 1013 z-score: 1146.0 E(): 0; 48.8% identity in 301 aa overlap and to TR:CAB59596 (EMBL:AL132662) Streptomyces coelicolor probable sugar transporter inner membrane protein SCF11.19, 316 aa; fasta scores: opt: 608 z-score: 690.8 E(): 4.4e-31; 32.8% identity in 320 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 4 3 15 15 [Reporter]SCO2471 (8P6)_PCR/5866 [Gene]SCO2471/10685 NC_003888 SC7A8.10c, possible secreted protein, len: 274 aa; similar to various hypothetical proteins, e.g. TR:P95216 (EMBL:Z86089) Mycobacterium tuberculosis hypothetical 26.2 kD protein, 247 aa; fasta scores: opt:413 z-score: 450.1 E(): 1.1e-17; 34.5% identity in 232 aa overlap. Contains possible N-terminal region signal peptide sequence 1 3 4 7 [Reporter]SCO1447 (15A14)_PCR/4104 [Gene]SCO1447/7465 NC_003888 SCL6.04c, possible ROK-family transcriptional regulatory protein, len: 399 aa; similar to TR:CAB71832 (EMBL:AL138662) Streptomyces coelicolor putative ROK-family regulatory protein SC8E4A.27, 409 aa; fasta scores: opt: 938 z-score: 965.1 E(): 0; 42.5% identity in 398 aa overlap and to SW:XYLR_ANATH (EMBL:Z69782) Anaerocellum thermophilum xylose repressor XylR, 399 aa; fasta scores: opt: 327 z-score: 341.8 E(): 1.3e-1; 22.2% identity in 397 aa overlap. Contains Pfam match to entry PF00480 ROK, ROK family and possible helix-turn-helix motif at residues 26..47 (+2.84 SD) 4 3 15 14 [Reporter]SCO7836 (9L6)_PCR/5865 [Gene]SCO7836/10683 NC_003888 SC8E7.33, unknown, len: 269 aa. This CDS lies within a sequence duplicated at both ends of the Streptomyces coelicolor chromosome. Identical to Streptomyces coelicolor TR:Q9S1Y3(EMBL:AL109973) hypothetical protein, SCJ30.06c (269 aa). Note the large overlap with the adjoining CDS. 1 3 4 6 [Reporter]SCO3340 (16M10)_PCR/4103 [Gene]SCO3340/7463 NC_003888 SCE7.07c, unknown, len: 400 aa; similar to TR:O53392 (EMBL:AL021841) hypothetical protein from Mycobacterium tuberculosis (243 aa) fasta scores; opt: 424, z-score: 497.4, E(): 2.2e-20, (35.8% identity in 226 aa overlap). 4 3 15 13 [Reporter]SCO6212 (10H6)_PCR/5864 [Gene]SCO6212/10681 NC_003888 SC9G1.02, possible permease, len: 471 aa; similar to SW:PBUX_BACSU (EMBL:X83878) Bacillus subtilis xanthine permease PubX, 438 aa; fasta scores: opt: 1060 z-score: 1156.8 E(): 0; 39.2% identity in 413 aa overlap to SW:URAA_ECOLI (EMBL:X73586) Escherichia coli uracil permease (uracil transporter) UraA, 429 aa; fasta scores: opt: 505 z-score: 554.2 E(): 1.7e-23; 28.9% identity in 422 aa overlap and to Streptomyces coelicolor St9G1.04, 462 aa; fasta scores: opt: 1668 z-score: 1511.0 E(): 0; 57.0% identity in 435 aa overlap. Contains match to Pfam entry Pfam match to entry PF00860 xan_ur_permease, Xanthine/uracil permeases family and match to Prosite entry PS01116 Xanthine/uracil permeases family signature 1 3 4 5 [Reporter]SCO1773 (17I10)_PCR/4102 [Gene]SCO1773/7461 NC_003888 SCI51.13c, probable L-alanine dehydrogenase, len: 371 aa; similar to many alanine dehydrogenases e.g. SW:DHA_MYCTU (EMBL:X63069), ald, Mycobacterium tuberculosis L-alanine dehydrogenase (371 aa), fasta scores; opt: 1569 z-score: 1722.1 E(): 0, 65.8% identity in 371 aa overlap. Contains Pfam match to entry PF01262 AlaDh_PNT, Alanine dehydrogenase/pyridine nucleotide transhydrogenase and PS00836 Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 1 1 3 4 4 [Reporter]SCO3551 (18E10)_PCR/4101 [Gene]SCO3551/7459 NC_003888 SCH5.14c, possible membrane spanning protein, len: 230aa; This CDS has been designated based on Hidden-Markoff Model and Frameplot predictions, lies in a region of high %GC DNA and contains a potential membrane spanning hydrophobic region. Glycine rich N-terminal. 4 3 15 11 [Reporter]SCO4123 (12P2)_PCR/5862 [Gene]SCO4123/10677 NC_003888 SCD72A.09c, possible two-component system response regulator, len: 222aa; similar to many eg. TR:O86632 (EMBL:AL031155) possible two-component regulator from Streptomyces coelicolor (222 aa) fasta scores; opt: 973, z-score: 1128.5, E(): 0, 69.1% identity in 220 aa overlap. Contains Pfam match to entry PF00072 response_reg, Response regulator receiver domain, Pfam match to entry PF00196 GerE, Bacterial regulatory proteins, luxR family and Prosite match to PS00622 Bacterial regulatory proteins, luxR family signature. 1 3 4 3 [Reporter]SCO4058 (19A10)_PCR/4100 [Gene]SCO4058/7457 NC_003888 2SCD60.24, unknown, len: 167 aa 4 3 15 10 [Reporter]SCO3033 (13L2)_PCR/5861 [Gene]SCO3033/10675 NC_003888 SCE34.14c, possible integral membrane regulatory protein, len: 1268 aa; N-terminal region identical to previously sequenced TR:Q53964 (EMBL:X62287) Streptomyces coelicolor regulatory protein WhiB, 914 aa and highly similar to TR:Q53785 (EMBL:L22864) Streptomyces aureofaciens transmembrane protein WhiB2, 1219 aa; fasta scores: opt: 4895 z-score: 4433.4 E(): 0; 62.0% identity in 1267 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 14 22 [Reporter]SCO0992 (1H14)_PCR/5851 [Gene]SCO0992/10657 NC_003888 2SCG2.05, possible cysteine synthase, len: 340 aa; similar to SW:CYSM_ALCEU (EMBL:X97499) Alcaligenes eutrophus cysteine synthase (EC 4.2.99.8) CysM, 339 aa; fasta scores: opt: 283 z-score: 331.0 E(): 6.5e-11; 30.3% identity in 277 aa overlap. Contains Pfam match to entry PF00291 PALP, Pyridoxal-phosphate dependent enzyme. Also contains TTA leucine codon (115), possible target for bldA regulation 4 3 14 21 [Reporter]SCO7035 (2D14)_PCR/5850 [Gene]SCO7035/10655 NC_003888 SC2C3.02, gabD, succinate-semialdehyde dehydrogenase (fragment), len: >422 aa; highly similar to SW:GABD_ECOLI (EMBL:M88334) Escherichia coli succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.16) GabD, 482 aa; fasta scores: opt: 1329 z-score: 1466.8 E(): 0; 48.9% identity in 415 aa overlap. Contains Pfam match to entry PF00171 aldedh, Aldehyde dehydrogenase family and match to Prosite entry PS00687 Aldehyde dehydrogenases glutamic acid active site,SC4G1.01, gabD, succinate-semialdehyde dehydrogenase (fragment), len: >90 aa; similar to SW:GABD_ECOLI (EMBL:M88334) Escherichia coli succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.16) GabD, 482 aa; fasta scores: opt: 320 z-score: 405.5 E(): 4.1e-15; 50.6% identity in 87 aa overlap 4 3 14 20 [Reporter]SCO5911 (3P10)_PCR/5849 [Gene]SCO5911/10653 NC_003888 SC10A5.16c, possible oligopeptide binding protein, len: 521; weak similarity to many eg. OPPA_SALTY P06202 periplasmic oligopeptide-binding protein (542 aa), fasta scores; opt: 343 z-score: 306.2 E(): 7.4e-10, 25.0% i dentity in 537 aa overlap. Contains N-terminal signal seque nce 4 3 14 18 [Reporter]SCO5448 (5H10)_PCR/5847 [Gene]SCO5448/10651 NC_003888 SC3D11.05c, probable ABC transporter, len: 583aa; similar to many eg. TR:Q54204 (EMBL:X89010) from the 5'-hydroxystreptomycin production/transport operon of Streptomyces glaucescens (591 aa) fasta scores; opt: 1687, z-score: 1814.2, E(): 0, 49.3% identity in 564 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, Pfam match to entry PF00664 ABC_membrane, ABC transporter transmembrane region and Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 1 8 15 [Reporter]SCO6689 (8D4)_PCR/1680 [Gene]SCO6689/3079 NC_003888 SC6G3.05, hypothetical protein, len: 588 aa; similar to TR:Q9RJY5 (EMBL:AL133210) Streptomyces coelicolor hypothetical protein SCF37.16, 667 aa; fasta scores: opt: 633 Z-score: 604.5 E(): 4.9e-26; 40.441% identity in 680 aa overlap. Probable CDS suggested by GC frameplot, positional base preference and amino acid composition 4 1 8 14 [Reporter]SCO6594 (8P24)_PCR/1679 [Gene]SCO6594/3077 NC_003888 SC8A6.15c, probable secreted protein, len: 602 aa; c ontains probable N-terminal signal sequence 4 1 8 13 [Reporter]SCO6469 (9L24)_PCR/1678 [Gene]SCO6469/3075 NC_003888 SC9C7.05c, possible acyl-CoA dehydrogenase, len: 401aa; similar to many from prokaryotes and eukaryotes egs. SW:ACDB_BACSU acyl-CoA dehydrogenase from Bacillus subtilis (379 aa) fasta scores; opt: 520, z-score: 1209.6, E(): 0, (38.9% identity in 386 aa overlap) and SW:ACDM_MOUSE acyl-CoA dehydrogenase from Mus musculus (mouse) (421 aa) fasta scores; opt: 484, z-score: 1178.1, E(): 0, (40.3% identity in 390 aa overlap). Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase. 4 1 8 12 [Reporter]SCO2316 (10H24)_PCR/1677 [Gene]SCO2316/3073 NC_003888 SCC53.07c, unknown, len: 365aa; 4 1 8 11 [Reporter]SCO4686 (11D24)_PCR/1676 [Gene]SCO4686/3071 NC_003888 SCD31.11, hypothetical protein, len: 103 aa; similar to TR:O54115 (EMBL:AL021529) Streptomyces coelicolor hypothetical 14.3 kD protein SC10A5.24c, 134 aa; fasta scores: opt: 353 z-score: 464.3 E(): 2e-18; 75.3% identity in 73 aa overlap 4 3 14 17 [Reporter]SCO5788 (6D10)_PCR/5846 [Gene]SCO5788/10649 NC_003888 SC4H2.09, unknown, len: 237 aa; similar to a hypothetical protein from Mycobacterium tuberculosis and M. leprae, TTR:O33233 (EMBL:Z98209) MTCY154.08C (231 aa), fasta scores; opt: 306 z-score: 320.4 E(): 1.3e-10, 33.2% identity in 241 aa overlap 4 3 14 16 [Reporter]SCO6757 (7P6)_PCR/5845 [Gene]SCO6757/10647 NC_003888 SC6A5.06, probable ABC transporter membrane component, len: 309aa; similar to many eg. TR:O66230 (EMBL:AB010150) ABC transporter membrane component from Escherichia coli (264 aa) fasta scores; opt: 346, z-score: 414.3, E(): 8.9e-16, (27.3% identity in 267 aa overlap). Contains Pfam match to entry PF01061 ABC2_membrane, ABC-2 type transporter, score 109.60, E-value 6.1e-29 and Prosite match to PS00211 ABC transporters family signature. Also contains probable membrane spanning hydrophobic regions. 4 3 14 15 [Reporter]SCO2466 (8L6)_PCR/5844 [Gene]SCO2466/10645 NC_003888 SC7A8.05, hypothetical protein, len: 201 aa; identical to C-terminus of previously sequenced TR:CAB51553 (EMBL:AJ244019) Streptomyces coelicolor A3(2) hypothetical 14.6 kD protein, 132 aa 4 3 14 14 [Reporter]SCO7735 (9H6)_PCR/5843 [Gene]SCO7735/10643 NC_003888 SC8D11.26, unknown, len: 70 aa 4 3 14 12 [Reporter]SCO2360 (11P2)_PCR/5841 [Gene]SCO2360/10641 NC_003888 SC8A.18, putative membrane protein, len: 293 aa; region similar to many of undefined function eg. SW:Q48630 (APL_LACLA) alkaline phosphatase like protein from Lactococcus lactis (242 aa) fasta scores; opt: 554, z-score: 549.3, E(): 3.9e-23, 46.4% identity in 194 aa overlap. Contains Pfam match to entry PF00597 DedA, DedA family. Contains possible hydrophobic membrane spanning regions 4 1 8 9 [Reporter]SCO3886 (13L20)_PCR/1674 [Gene]SCO3886/3067 NC_003888 StH24.08, possible partitioning or sporulation protein, len: 275 aa; previously sequenced as TR:O07328 (EMBL:Y16311) Streptomyces coelicolor hypothetical protein (255 aa), but with a different stop codon position. Similar to many e.g. SW:SOJ_BACSU (EMBL:D26185), SOJ, Bacillus subtilis sporulation protein (253 aa), fasta scores; opt: 899 z-score: 1077.0 E(): 0, 56.6% identity in 251 aa overlap, TR:O05189 (EMBL:U87804), parA, Caulobacter crescentus chromosome partitioning protein (266 aa) (49.2% identity in 248 aa overlap) and TR:O53596 (EMBL:AL021426), parB, Mycobacterium tuberculosis hypothetical protein (347 aa) (64.3% identity in 249 aa overlap). Contains Pfam match to entry PF00991 ParA, ParA family ATPase, score 123.30, E-value 4.4e-33. Contains probable coiled-coil from 86 to 114 (29 residues) (Max score: 1.424, probability 0.80) 4 1 8 8 [Reporter]SCO3376 (14H20)_PCR/1673 [Gene]SCO3376/3065 NC_003888 SCE94.27c, possible acetyltransferase, len: 169aa; similar to hypothetical proteins eg. TR:O33289 (EMBL:AL008967) from Mycobacterium tuberculosis (174 aa) fasta scores; opt: 656, z-score: 842.4, E(): 0, (60.4% identity in 164 aa overlap). Also some similarity to SW:ARGA_ECOLI ArgA, amino-acid acetyltransferase from Escherichia coli (443 aa) fasta scores; opt: 192, z-score: 248.7, E(): 1.5e-06, (29.0% identity in 138 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 4 1 8 7 [Reporter]SCO0326 (15D20)_PCR/1672 [Gene]SCO0326/3063 NC_003888 SCF12.05, hypothetical protein, len: 322 aa; similar to TR:O54173 (EMBL:AL021411) hypothetical protein from Streptomyces coelicolor (382 aa) fasta scores; E(): 6.7e-12, 34.2% identity in 371 aa overlap. 4 1 8 6 [Reporter]SCO0663 (16P16)_PCR/1671 [Gene]SCO0663/3061 NC_003888 SCF91.23, possible 2-hydroxyacid-family dehydrogenase, len: 343 aa. Similar in parts to several D-3-phosphoglycerate dehydrogenases e.g. Mycobacterium tuberculosis SW:SERA_MYCTU (EMBL:AL021287) (528 aa), fasta scores opt: 450 z-score: 536.4 E(): 1.7e-22 34.1% identity in 261 aa overlap. Also similar to Streptomyces coelicolor TR:CAB55710(EMBL:AL117387) possible dehydrogenase SCF41.07 (339 aa), fasta scores opt: 927 z-score: 1106.0 E(): 0 48.6% identity in 325 aa overlap. Contains a Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases and a Prosite hit to PS00671 D-isomer specific 2-hydroxyacid dehydrogenases signature 3. 4 3 14 11 [Reporter]SCO4119 (12L2)_PCR/5840 [Gene]SCO4119/10639 NC_003888 SCD72A.05, possible NADH dehydrogenase, len: 442aa; similar to many eg. TR:Q9ZC17 (EMBL:AL033505) putative dehydrogenase from Streptomyces coelicolor SC1E6.05 (629 aa) fasta scores; opt: 893, z-score: 1049.2, E(): 0, 38.5% identity in 452 aa overlap and SW:P00393 (DHNA_ECOLI) NADH dehydrogenase Ndh (EC 1.6.99.3) from Escherichia coli (433 aa) fasta scores; opt: 404, z-score: 477.0, E(): 4.2e-19, 27.0% identity in 389 aa overlap. 4 3 14 10 [Reporter]SCO3186 (13H2)_PCR/5839 [Gene]SCO3186/10637 NC_003888 SCE22.03, possible ABC transporter integral membrane protein, len: 421 aa; similar to TR:O50532 (EMBL:AL009204) Streptomyces coelicolor hypothetical 41.1 kD protein SC9B10.25c, 393 aa; fasta scores: opt: 721 z-score: 787.1 E(): 0; 34.7% identity in 363 aa overlap. Contains match to Prosite entry PS00211 ABC transporters family signature and possible hydrophobic membrane spanning regions 4 3 14 9 [Reporter]SCO1609 (14D2)_PCR/5838 [Gene]SCO1609/10635 NC_003888 SCI35.31, unknown, len: 402 aa; similar to a hypothetical protein from M. tuberculosis TR:O06802 (EMBL:Z95890) MTCY28.35 (414 aa), fasta scores; opt: 1341 z-score: 1649.4 E(): 0, 53.8% identity in 398 aa overlap 4 3 14 8 [Reporter]SCO0836 (14P22)_PCR/5837 [Gene]SCO0836/10633 NC_003888 SCF43A.26c, conserved possible membrane protein, len: 333 aa; unknown function, similar to (or to part of) many hypothetical proteins of different sizes e.g. TR:Q55987 (EMBL:D64005) Synechocystis sp. hypothetical protein (617 aa), fasta scores; opt: 581 z-score: 633.2 E(): 6.2e-28, 40.1% identity in 232 aa overlap and TR:O05781 (EMBL:Z95150) Mycobacterium tuberculosis hypothetical protein (308 aa) (34.9% identity in 301 aa overlap). Contains hydrophobic, possible membrane-spanning region. Contains Pfam match to entry PF00924 UPF0003, Uncharacterized protein family UPF0003 4 3 14 7 [Reporter]SCO0511 (15L22)_PCR/5836 [Gene]SCO0511/10631 NC_003888 SCF6.07, possible dehydrogenase (putative secreted protein), len: 252 aa. Highly similar to many e.g. Pseudomonas paucimobilis SW:LINX_PSEPA (EMBL; D23722) 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (EC 1.1.-.-) (2,5-ddol dehydrogenase) LinX (250 aa), fasta scores opt: 532 z-score: 558.4 E(): 9.7e-24 40.0% identity in 245 aa overlap. Also similar to many Streptomyces coelicolor putative oxidoreductases e.g. TR:O88068 (EMBL; AL031541) putative dehydrogenase SCI35.33C (260 aa), fasta scores opt: 488 z-score: 438.3 E(): 4.1e-19 38.7% identity in 256 aa overlap. Contains a Prosite hit to PS00061 Short-chain dehydrogenases/reductases family signature and Pfam hits to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and Pfam match to entry PF00106 adh_short, short chain dehydrogenase. 4 1 8 5 [Reporter]SCO0883 (17L16)_PCR/1670 [Gene]SCO0883/3059 NC_003888 SCM1.16, probable polypeptide deformylase (EC 3.5.1.31), len: 218 aa. Similar to many members of the polypeptide deformylase family e.g. Thermus aquaticus SW:DEF_THETH (EMBL:X79087) polypeptide deformylase (EC 3.5.1.31) (192 aa), fasta scores opt: 321 z-score: 384.1 E(): 4.8e-14 39.0% identity in 164 aa overlap. Contains a Pfam match to entry PF01327 Pep_deformylase, Polypeptide deformylase. 4 1 8 4 [Reporter]SCO3579 (18H16)_PCR/1669 [Gene]SCO3579/3057 NC_003888 SCH17.13c, probable transcriptional regulator, len: 112 aa; similar to many transcriptional regulators from Streptomyces e.g. TR:Q53963 (EMBL:X62287), WhiB, S.coelicolor transcriptional regulator essential for sporulation (87 aa), fasta scores; opt: 230 z-score: 307.8 E(): 8.1e-10, 50.0% identity in 64 aa overlap. Highest similarity is to TR:O69649 (EMBL:AL022121) Mycobacterium tuberculosis putative regulatory protein (100 aa) (67.9% identity in 78 aa overlap) 4 1 8 3 [Reporter]SCP1.171 (19D16)_PCR/1668 [Gene]SCP1.171/3055 NC_003888 SCP1.171, possible integral membrane protein, len: 297aa; similar to TR:Q9L0R6 (EMBL:AL160312) putative integral membrane protein from Streptomyces coelicolor (302 aa) fasta scores; opt: 379, z-score: 425.8, E(): 3e-16, 30.3% identity in 297 aa overlap. Contains possible membrane-spanning hydrophobic regions. 4 1 8 2 [Reporter]SCO5996 (20L12)_PCR/1667 [Gene]SCO5996/3053 NC_003888 SCBAC16H6.31, possible membrane protein, len: 937 aa: similar to TR:O54182 (EMBL:AL021411) hypothetical protein SC7H1.23 from Streptomyces coelicolor (913 aa) fasta scores; opt: 1128, Z-score: 1138.9, 29.418% identity (31.524% ungapped) in 928 aa overlap. C-terminal region contains 13 Pfam matches to entry PF00400 WD40, WD domain, G-beta repeat; 5 Prosite matches to PS00678 Trp-Asp (WD) repeats signature and a Prosite match to PS00626 Regulator of chromosome condensation (RCC1) signature 2 4 1 7 22 [Reporter]SCO1229 (1L8)_PCR/1665 [Gene]SCO1229/3051 NC_003888 2SCG1.04, possible reductase, len: 353 aa; similar to TR:CAB90862 (EMBL:AL355752) Streptomyces coelicolor putative reductase SC10B7.09, 366 aa; fasta scores: opt: 182 z-score: 207.8 E(): 0.00042; 31.9% identity in 295 aa overlap 4 3 14 6 [Reporter]SCO1815 (16H22)_PCR/5835 [Gene]SCO1815/10629 NC_003888 SCI28.09c, fabG, probable 3-oxacyl-(acyl-carrier-protein) reductase, len: 234 aa; similar to many e.g. SW:FABG_MYCTU 3-oxacyl-(acyl-carrier-protein) reductase from Mycobacterium tuberculosis (247 aa) fasta scores; opt: 906, z-score: 997.3, E(): 0, (57.3% identity in 234 aa overlap). Contains Pfam match to entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature. 4 3 14 5 [Reporter]SCO2961 (17D22)_PCR/5834 [Gene]SCO2961/10627 NC_003888 SCE59.20c, possible integral membrane protein, len: 401 aa; similar to TR:Q9XAP7 (EMBL:AL078618) Streptomyces coelicolor putative integral membrane transferase SC10A7.28c, 363 aa; fasta scores: opt: 397 z-score: 390.6 E(): 2.4e-14; 46.6% identity in 369 aa overlap. Contains possible hydrophobic membrane spanning regions and match to Prosite entry PS00038 Myc-type, 'helix-loop-helix' dimerization domain signature 4 3 14 4 [Reporter]SCO4033 (18P18)_PCR/5833 [Gene]SCO4033/10625 NC_003888 2SC10A7.37, possible membrane protein, len: 96 aa. Contains possible hydrophobic membrane spanning region at C-terminal domain 4 3 14 3 [Reporter]SCO1875 (19L18)_PCR/5832 [Gene]SCO1875/10623 NC_003888 SCI39.22, possible secreted penicillin-binding protein, len: 484 aa; similar to TR:Q9L656 (EMBL:AF241575) Streptomyces griseus PbpA, 485 aa; fasta scores: opt: 1581 Z-score: 1674.1 bits: 319.2 E(): 1.2e-85; 51.633% identity in 490 aa overlap and to TR:Q9XA17 (EMBL:AL079308) Streptomyces coelicolor putative penicillin binding protein SCH69.17, 490 aa; fasta scores: opt: 1649 Z-score: 1745.9 bits: 332.5 E(): 1.2e-89; 52.965% identity in 489 aa overlap. Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop). Also contains N-terminal region signal peptide sequence 4 3 14 2 [Reporter]SCO3965 (20D18)_PCR/5831 [Gene]SCO3965/10621 NC_003888 SCBAC25E3.02c, conserved hypothetical protein, len: 178aa: similar to many eg. TR:BAB50242 (EMBL:AP003001) MLL3326 protein from Rhizobium loti (179 aa) fasta scores; opt: 241, Z-score: 269.7, 38.333% identity (41.441% ungapped) in 120 aa overlap. 4 1 7 21 [Reporter]SCO5849 (2H8)_PCR/1664 [Gene]SCO5849/3049 NC_003888 SC9B10.16, agaS, len: 385 aa; similar to AGAS_ECOLI P42907 agas protein (384 aa) (possible tagatose-6-phosphate ketose/aldose isomerase), fasta scores; opt: 960 z-score: 1350.4 E(): 0, 45.9% identity in 366 aa overlap 4 1 7 20 [Reporter]SCO5244 (3D8)_PCR/1663 [Gene]SCO5244/3047 NC_003888 2SC7G11.06c, prs1, anti-sigma factor, len: 137 aa; identical to previously sequenced TR:Q9R3X8 (EMBL:AJ249450) Streptomyces coelicolor A3(2) putative regulator of Sig1, Prs1 protein or UshX, 137 aa 4 1 7 19 [Reporter]SCO6613 (4P4)_PCR/1662 [Gene]SCO6613/3045 NC_003888 SC1F2.10, unknown, len: 135 aa; similar to hypothetical proteins from several organisms e.g. YTFH_ECOLI (156 aa), fasta scores; opt: 315 z-score: 460.2 E(): 2.2e-18, 40.5% identity in 116 aa overlap 4 1 7 18 [Reporter]SCO6567 (5L4)_PCR/1661 [Gene]SCO6567/3043 NC_003888 SC3F9.02, ABC transporter ATP binding protein, len:505 aa; similar to many sugar transport proteins e.g. ARAG_ECOLI l-arabinose transport atp-binding protein (504 aa), fasta scores; opt: 1312 z-score: 1412.8 E(): 0, 42.1% identity in 501 aa overlap andRBSA_HAEIN ribose transport atp-binding protein rbsA (493 aa), fasta scores; opt: 1160 z-score: 1216.7 E(): 0, 40.7% identity in 489 aa overlap. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature and Pfam match to entry ABC_tran PF00005, ABC transporters, score 197.70 4 1 7 17 [Reporter]SCO6649 (6H4)_PCR/1660 [Gene]SCO6649/3041 NC_003888 SC4G2.23, unknown, len: 224 aa 4 3 13 22 [Reporter]SCO1289 (1D14)_PCR/5829 [Gene]SCO1289/10619 NC_003888 2SCG18.36, probable gntR-family regulatory protein, len: 507 aa; similar to TR:CAB89055 (EMBL:AL353872) Streptomyces coelicolor putative gntR-family regulator SC5G8.04, 462 aa; fasta scores: opt: 1289 z-score: 1152.7 E(): 0; 49.3% identity in 436 aa overlap and to SW:MOCR_RHIME (EMBL:X78503) Rhizobium meliloti probable rhizopine catabolism regulatory protein MocR, 493 aa; fasta scores: opt: 584 z-score: 527.8 E(): 6.5e-22; 34.8% identity in 451 aa overlap. Contains Pfam match to entry PF00392 gntR, Bacterial regulatory proteins, gntR family 4 3 13 21 [Reporter]SCO5868 (2P10)_PCR/5828 [Gene]SCO5868/10617 NC_003888 SC2E9.09, dut, probable deoxyuridine 5'-triphosphate nucleotidohydrolase, len: 183 aa; similar to many eg. DUT_LYCES P32518 deoxyuridine 5'-triphosphate nucleotidohydrolase (169 aa), fasta scores; opt: 299 z-score: 371.4 E(): 1.7e-13, 37.9% identity in 140 aa overlap 4 3 13 20 [Reporter]SCO5906 (3L10)_PCR/5827 [Gene]SCO5906/10615 NC_003888 SC10A5.11, probable transcriptional regulator, len: 198 aa; similar to many members of the tetR family eg. TR:P72569 (EMBL:U58281) TetR homologue WhiR from Streptomyces roseosporus (179 aa), fasta scores; opt: 367 z-score: 569.5 E(): 1.6e-24, 38.3% identity in 175 aa overlap. Contains pr obable helix-turn-helix motif at aa 37 to 58 (Score 1635, +4.76SD) 4 3 13 19 [Reporter]SCO5556 (4H10)_PCR/5826 [Gene]SCO5556/10613 NC_003888 SC1C2.37, histone-like DNA binding protein, len: 218 aa; N-terminus is highly simialar to many bacterial HU proteins e.g. DBHB_ECOLI DNA-binding protein HU-beta (NS1) (HU-1) (90 aa), fasta scores; opt: 230 z-score: 300.3 E(): 1.8e-09, 40.0% identity in 90 aa overlap, and contains PS00045 Bacterial histone-like DNA-binding proteins signature and Pfam match to entry PF00216 Bac_DNA_binding, Bacterial DNA-binding proteins, score 104.40, E-value 9.2e-29. C-terminus contains 18 degenerate repeats of an (A /T)(3-5)KK motif. A similar C-terminal extension is also present in the M. tuberculosis homologue TR:P95109 (EMBL:Z83018) dna-binding protein MTCY349.01 (214 aa), fasta scores; opt: 563 z-score: 543.5 E(): 5e-23, 50.9% identity in 220 aa overlap, and to a lesser extent in the M. leprae homologue. The repeats are simlar to those in hrdB (SC5B8.10, 45.4% identity in 97 aa overlap) 4 3 13 18 [Reporter]SCO6412 (5D10)_PCR/5825 [Gene]SCO6412/10611 NC_003888 SC1A6.01, probable aminotransferase, partial CDS, len: 190 aa; similar to many members of the class-III family of pyridoxal-phosphate-dependent aminotransferases e.g. ARGD_ANASP P54752 anabaena sp. acetylornithine aminotransferase (427 aa), fasta scores; opt: 315 z-score: 402.3 E(): 2.8e-15, 36.0% identity in 189 aa overlap. Contains Pfam matchto entry aminotran_3 PF00202, Aminotransferases class-III pyridoxal-phosphate, score 147.18,SC3C8.31, probable aminotransferase, partial CDS, len: > 270 aa; similar to many members of the class-III pyridoxal-phosphate-dependent aminotransferase family, e.g. ARGD_CORGL acetylornithine aminotransferase (389 aa), fasta scores; opt: 202 z-score: 588.9 E(): 1.5e-25, 36.9% identity in 271 aa overlap. Contains Pfam match to entry aminotran_3 PF00202, Aminotransferases class-III pyridoxal-phosphate, score 171.39. Continues as SC1A6.01 4 3 13 17 [Reporter]SCO5827 (6P6)_PCR/5824 [Gene]SCO5827/10609 NC_003888 SC5B8.17, probable transmembrane transport protein, len: 530 aa; member of the sodium:solute symporter family, similar to many e.g. YJCG_ECOLI P32705 hypothetical protein , (549 aa), fasta scores; opt: 935 z-score: 1534.8 E(): 0, 43.6% identity in 530 aa overlap. Contains Pfam match to en try SSF PF00474, Sodium:solute symporter family, score 338. 37 4 3 13 16 [Reporter]SCO6753 (7L6)_PCR/5823 [Gene]SCO6753/10607 NC_003888 SC6A5.02, probable nucleotide sugar-1-phosphate transferase, len: 250aa; similar to many eg. TR:O05171 (EMBL:Y11985) dNDP-glucose synthase from the avilamycin biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 (355 aa) fasta scores; opt: 192, z-score: 226.4, E(): 2.6e-05. (26.9% identity in 242 aa overlap). Contains Pfam match to entry PF00483 NTP_transferase, Nucleotidyl transferase. 4 3 13 15 [Reporter]SCO4756 (8H6)_PCR/5822 [Gene]SCO4756/10605 NC_003888 SC6G4.34, unknown, len: 118 aa; similar to a hypothe tical protein from Rhizobium meliloti TR:Q52996 (EMBL:L3926 5) (100 aa), fasta scores; opt: 130 z-score: 222.8 E(): 3.8 e-05, 28.8% identity in 73 aa overlap 4 3 13 14 [Reporter]SCO7731 (9D6)_PCR/5821 [Gene]SCO7731/10603 NC_003888 SC8D11.22, unknown, len: 183 aa 4 3 13 13 [Reporter]SCO6262 (10P2)_PCR/5820 [Gene]SCO6262/10601 NC_003888 SCAH10.27, possible helicase, len: 977 aa; similar to TR:O53499 (EMBL:AL021924) Mycobacterium tuberculosis helicase, 1013 aa; fasta scores: opt: 1308 z-score: 1370.7 E(): 0; 39.7% identity in 939 aa overlap. Contains two matches to Pfam entries PF00176 SNF2_N, SNF2 and others N-terminal domain and PF00271 helicase_C, Helicases conserved C-terminal domain 4 1 7 9 [Reporter]SCO0229 (13H20)_PCR/1652 [Gene]SCO0229/3027 NC_003888 SCJ9A.08, probable oxidoreductase (putative secreted protein), len: 282 aa; similar to many e.g. TR:CAB51136 (EMBL:Y18817) oxidoreductase of short chain from Streptomyces coelicolor (276 aa) fasta scores; opt: 615, z-score: 697.7, E(): 1.7e-31, (40.9% identity in 274 aa overlap). Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase and Prosite match to PS00061 Short-chain dehydrogenases/reductases family signature 4 1 7 8 [Reporter]SCO0061 (14D20)_PCR/1651 [Gene]SCO0061/3025 NC_003888 SCJ4.42c, unknown, len: 434 aa; similar to SW:YDEG_SCHPO hypothetical protein from Schizosaccharomyces pombe (fission yeast) (509 aa) fasta scores; opt: 665, z-score: 791.0, E(): 0, (37.6% identity in 423 aa overlap) and to TR:Q9KEQ9 (EMBL:AP001509) Bacillus halodurans BH0790 protein, 454 aa; fasta scores: opt: 1262 Z-score: 1468.0 bits: 280.8 E(): 3.9e-74; 45.433% identity in 416 aa overlap 4 1 7 7 [Reporter]SCO0973 (15P16)_PCR/1650 [Gene]SCO0973/3023 NC_003888 SCM11.28, probable integral membrane protein, len: 419 aa; similar to various putative integral membran proteins, e.g. TR:Q9XAN8 (EMBL:AL079355) Streptomyces coelicolor putative integral membrane protein SC4C6.02c, 317 aa; fasta scores: opt: 674 z-score: 607.8 E(): 1.8e-26; 43.6% identity in 326 aa overlap 4 1 7 6 [Reporter]SCO3780 (16L16)_PCR/1649 [Gene]SCO3780/3021 NC_003888 SCH63.27, probable sugar hydrolase, len: 556 aa; similar to SW:TREC_ECOLI (EMBL:U06195) Escherichia coli trehalose-6-phosphate hydrolase (EC 3.2.1.93) TreC, 551 aa; fasta scores: opt: 833 z-score: 927.7 E(): 0; 35.4% identity in 557 aa overlap and to TR:CAB90876 (EMBL:AL355753) Streptomyces coelicolor aglA, alpha-glucosidase SC10B7.23c, 577 aa; fasta scores: opt: 1146 z-score: 1084.7 E(): 0; 56.8% identity in 572 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase 4 1 7 5 [Reporter]SCO3724 (17H16)_PCR/1648 [Gene]SCO3724/3019 NC_003888 SCH22A.02c, hypothetical protein, len: 160 aa; similar to TR:Q9X7Q5 (EMBL:AL049587) Streptomyces coelicolor hypothetical 17.1 kD protein SC5F2A.32, 161 aa; fasta scores: opt: 256 z-score: 310.3 E(): 7.3e-10; 39.4% identity in 160 aa overlap 4 1 7 4 [Reporter]SCO0878 (18D16)_PCR/1647 [Gene]SCO0878/3017 NC_003888 SCM1.11, possible hydrolase, len: 292 aa. Highly similar to several including: Streptomyces peucetius TR:Q54809(EMBL:L40425) 10-carbomethoxy-13-deoxycarminomycin esterase (298 aa), fasta scores opt: 670 z-score: 757.9 E():0 40.3% identity in 288 aa overlap and Streptomyces purpurascens TR:Q54528(EMBL:U10405:) possible C-C hydrolase, RdmC (298 aa) fasta scores opt: 709 z-score: 801.6 E():0 41.2% identity in 289 aa overlap. Contains a Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold. 4 1 7 3 [Reporter]SCP1.275 (19P12)_PCR/1646 [Gene]SCP1.275/3015 NC_003888 SCP1.275, unknown, doubtful CDS, len: 121aa; 4 1 6 22 [Reporter]SCO5261 (1H8)_PCR/1643 [Gene]SCO5261/3013 NC_003888 2SC7G11.23, probable malate oxidoreductase, len: 409 aa; similar to SW:MAOX_BACST (EMBL:M19485) Bacillus stearothermophilus NAD-dependent malic enzyme (EC 1.1.1.38), 478 aa; fasta scores: opt: 1385 z-score: 1470.9 E(): 0; 55.0% identity in 391 aa overlap. Contains 2x Pfam matches to entry PF00390 malic, Malic enzyme 4 1 6 21 [Reporter]SCO2792 (2D8)_PCR/1642 [Gene]SCO2792/3011 NC_003888 SCC105.23, adpA, araC-family transcriptional regulator, len: 398 aa; highly similar to TR:BAA86265 (EMBL:AB023785) Streptomyces griseus AdpA, 405 aa; fasta scores: opt: 2219 z-score: 2270.5 E(): 0; 84.4% identity in 410 aa overlap and to TR:Q9XA73 (EMBL:AL096822) Streptomyces coelicolor putative araC-family transcriptional regulator SCGD3.05, 334 aa; fasta scores: opt: 878 z-score: 904.2 E(): 0; 45.7% identity in 313 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family and match to Prosite entry PS00041 Bacterial regulatory proteins, araC family signature. Contains possible helix-turn-helix motif 4 2 15 16 [Reporter]SCO5697 (7D11)_PCR/3851 [Gene]SCO5697/6999 NC_003888 SC5H4.21, hypothetical protein, len: 287 aa; similar to TR:O33349 (EMBL:AL008883) Mycobacterium tuberculosis hypothetical 30.3 kD protein MTV003.13c, 284 aa; fasta scores: opt: 798 z-score: 942.8 E(): 0; 48.0% identity in 269 aa overlap 4 1 6 20 [Reporter]SCO4707 (3P4)_PCR/1641 [Gene]SCO4707/3009 NC_003888 SCD31.32, rplV, 50S ribosomal protein L22, len: 125 aa; highly similar to SW:RL22_BACST (EMBL:X54994) Bacillus stearothermophilus 50S ribosomal protein L22, RplV, 113 aa; fasta scores: opt: 441 z-score: 566.9 E(): 3.8e-24; 64.0% identity in 114 aa overlap. Contains Pfam match to entry PF00237 Ribosomal_L22, Ribosomal protein L22 and match to Prosite entry PS00464 Ribosomal protein L22 signature 4 2 15 15 [Reporter]SCO2479 (8P7)_PCR/3850 [Gene]SCO2479/6997 NC_003888 SC7A8.18, possible membrane protein, len: 180 aa. Contains possible hydrophobic membrane spanning regions both at N- and C-terminal domains 4 1 6 18 [Reporter]SCO6361 (5H4)_PCR/1639 [Gene]SCO6361/3007 NC_003888 SC3A7.29, probable integral membrane protein, len: 279 aa. Contains possible hydrophobic membrane spanning regions 4 2 15 14 [Reporter]SCO6976 (9L7)_PCR/3849 [Gene]SCO6976/6995 NC_003888 SC8F11.02c, unknown, len: 298 aa. Similar to the Bacillus subtilis SW:IOLB_BACSU(EMBL:D14399) hypothetical protein, IolB, found within the inositol utilisation operon (271 aa), fasta scores opt: 443 z-score: 516.6 E(): 2.4e-21 34.9% identity in 281 aa overlap 4 1 6 17 [Reporter]SCO6645 (6D4)_PCR/1638 [Gene]SCO6645/3005 NC_003888 SC4G2.19, probable transport system permease protein, len: 601 aa; similar to many members of the oppBC family e.g. NIKB_ECOLI nickel transport system permease protein (314 aa), fasta scores; opt: 405 z-score: 322.1 E(): 1.1e-10, 33.4% identity in 290 aa overlap 4 2 15 12 [Reporter]SCO2538 (11D7)_PCR/3847 [Gene]SCO2538/6993 NC_003888 SCC77.05, unknown, len: 132 aa. Similar to Mycobacterium tuberculosis TR:O05833(EMBL:Z95208) hypothetical 11.1 KD protein (113 aa), fasta scores opt: 254 z-score: 298.3 E(): 3.2e-09 53.1% identity in 96 aa overlap and Mycobacterium leprae TR:Q49767(EMBL:U00016) hypothetical 11.1 KD protein (108 aa), fasta scores opt: 186 z-score: 224.0 E(): 4.4e-05 39.4% identity in 99 aa overlap. 4 1 6 16 [Reporter]SCO4859 (6P24)_PCR/1637 [Gene]SCO4859/3003 NC_003888 SC5G8.27c, unknown, len: 183 aa; similar to TR:Q9Z593 (EMBL:AL035478) hypothetical protein from Streptomyces coelicolor (191 aa) fasta scores; opt: 164, z-score: 195.7, E(): 0.0019, 32.6% identity in 187 aa overlap. 4 2 15 11 [Reporter]SCO4131 (12P3)_PCR/3846 [Gene]SCO4131/6991 NC_003888 SCD72A.17c, possible membrane protein, len: 420aa; contains possible membrane-spanning hydrophobic region. 4 1 6 15 [Reporter]SCO2135 (7L24)_PCR/1636 [Gene]SCO2135/3001 NC_003888 SC6G10.08c, possible secreted protein, len: 338 aa; similar to TR:O69580 (EMBL:AL022602) putative secreted protein from Mycobacterium leprae (374 aa) fasta scores; opt: 382, z-score: 384.3, E(): 4.2e-14, (32.4% identity in 358 aa overlap). Also similar to several examples of Listeria P60 a major extracellular protein involved in the invasion of non-professional phagocytic cells eg. SW:P60_LISIV from Listeria ivanovii (524 aa) fasta scores; opt: 337, z-score: 338.3, E(): 1.5e-11, (29.1% identity in 309 aa overlap). Contains Pfam match to entry PF00877 NLPC_P60, NLP/P60 family and the C-terminus, a probable membrane spanning hydrophobic region towards the N-terminus and two possible coiled coil regions. NOTE: highly similar to upstream gene, 6G10.09c, indicating a duplication event; fasta scores: opt: 1042, z-score: 843.1, E(): 0, (49.9% identity in 339 aa overlap). 4 2 15 10 [Reporter]SCO3042 (13L3)_PCR/3845 [Gene]SCO3042/6989 NC_003888 SCE34.23, possible transcriptional regulator, len: 396 aa; similar to TR:O86796 (EMBL:AL031317) Streptomyces coelicolor putative transcriptional regulator SC6G4.33, 360 aa; fasta scores: opt: 792 z-score: 875.9 E(): 0; 43.3% identity in 363 aa overlap, to TR:O68907 (EMBL:AF05830) Streptomyces roseofulvus FrnA, 770 aa; fasta scores: opt: 686 z-score: 739.2 E(): 0; 41.3% identity in 283 aa overlap, to Streptomyces coelicolor SCE34.26, 507 aa; fasta scores: opt: 709 z-score: 659.1 E(): 3.4e-31; 36.4% identity in 357 aa overlap, StE34.25, 507 aa; fasta scores: opt: 671 z-score: 624.0 E(): 3e-29;33.2% identity in 365 aa overlap, to SCE34.24, 472 aa; fasta scores: opt: 666 z-score: 619.9 E(): 5.2e-29; 38.0% identity in 324 aa overlap and to SCE34.27 (partial), >356 aa; fasta scores: opt: 554 z-score: 518.2 E(): 2.4e-23; 35.7% identity in 361 aa overlap. Contains a possible hydrophobic membrane spanning region at N-terminal domain 4 2 15 9 [Reporter]SCO3401 (14H3)_PCR/3844 [Gene]SCO3401/6987 NC_003888 SCE9.08, folK, probable hydroxymethyldihydropteridine pyrophosphokinase, len: 203 aa; similar to many e.g. SW:HPPK_ECOLI (EMBL:L06495), FolK, Escherichia coli 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (158 aa), fasta scores; opt: 351 z-score: 426.9 E(): 1.9e-16, 42.0% identity in 138 aa overlap. Putative start codon suggested by GC frame plot and the presence of a potential RBS, resulting in a short N-terminal extension not present in homologs. There is an alternative start codon at position 17. Contains Pfam match to entry PF01288 HPPK, 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK), score 145.80, E-value 7.5e-40 4 2 15 8 [Reporter]SCO1154 (15D3)_PCR/3843 [Gene]SCO1154/6985 NC_003888 SCG8A.08c, unknown, len: 323aa 4 2 15 7 [Reporter]SCO3763 (15P23)_PCR/3842 [Gene]SCO3763/6983 NC_003888 SCH63.10c, hypothetical protein, len: 694 aa; N-terminal region similar to SW:TERD_ALCSP (EMBL:M20238) Alcaligenes sp. tellurium resistance protein TerD (plasmid determined), 192 aa; fasta scores: opt: 420 z-score: 446.6 E(): 2.3e-17; 39.8% identity in 186 aa overlap and C-terminal region similar to C-terminal region of TR:Q9X843 (EMBL:AL049727) Streptomyces coelicolor putative transferase (fragment) SC9B1.24, 317 aa; blastp scores: Score = 76 (26.8 bits), Expect = 7.3, Sum P(2) = 1.0; Identities = 52/165 (31%), Positives = 72/165 (43%). Contains Pfam match to entry PF02342 TerD at N-terminal region 4 2 15 6 [Reporter]SCO1149 (16L23)_PCR/3841 [Gene]SCO1149/6981 NC_003888 SCG8A.03, unknown, len: 156aa; strongly similar to another hypothetical protein from Streptomyces coelicolor TR:CAB69691 (EMBL:AL137165) (158 aa) fasta scores; opt: 570, z-score: 640.3, E(): 3.2e-28, 59.5% identity in 153 aa overlap. Hydrophilic 4 2 15 5 [Reporter]SCO0464 (17H23)_PCR/3840 [Gene]SCO0464/6979 NC_003888 SCF76.04c, possible integral membrane protein, len: 565 aa. Similar in parts to many hypothetical proteins e.g. Streptomyces lividans TR:O86875 (EMBL; U22894) hypothetical 57.9 KD protein (547 aa), fasta scores opt: 689 z-score: 806.6 E(): 0 41.7% identity in 439 aa overlap and Streptomyces coelicolor TR:O86807 (EMBL; AL031031) putative regulatory protein (916 aa), fasta scores opt: 329 z-score: 381.2 E(): 7.1e-14 26.5% identity in 582 aa overlap. Contains a Pfam match to entry PF01590 GAF, GAF domain and possible membrane spanning hydrophobic domains. 4 2 15 4 [Reporter]SCO1071 (18D23)_PCR/3839 [Gene]SCO1071/6977 NC_003888 SCG22.17c, possible two component system sensor kinase, len: 415 aa; similar to TR:Q9X8S1 (EMBL:AL049754) Streptomyces coelicolor putative two component system sensor kinase SCH10.32c, 441 aa; fasta scores: opt: 723 z-score: 761.0 E(): 0; 40.6% identity in 382 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 15 3 [Reporter]SCO6100 (19P19)_PCR/3838 [Gene]SCO6100/6975 NC_003888 SCBAC1A6.24c, cysH, phosphoadenosine phosphosulfate reductase, len: 236 aa; similar to SW:CYH1_BACSU (EMBL:U76751) Bacillus subtilis phosphoadenosine phosphosulfate reductase (EC 1.8.99.4) CysH, 233 aa; fasta scores: opt: 618 z-score: 746.2 E(): 0; 44.3% identity in 210 aa overlap. Contains Pfam match to entry PF01507 PAPS_reduct, Phosphoadenosine phosphosulfate reductase family 4 2 15 2 [Reporter]SCO5106 (20H19)_PCR/3837 [Gene]SCO5106/6973 NC_003888 SCBAC31E11.02c, shdB2, probable succinate dehydrogenase iron-sulfur subunit, len: 259 aa; highly similar to TR:AAK44479 (EMBL:AE006934) Mycobacterium tuberculosis ferredoxin, 2Fe-2S MT0261, 248 aa; fasta scores: opt: 1355 Z-score: 1588.1 bits: 301.4 E(): 7.7e-81; 74.900% identity in 251 aa overlap and to SW:DHSB_BACSU (EMBL:M13470) Bacillus subtilis succinate dehydrogenase iron-sulfur protein SdhB, 252 aa; fasta scores: opt: 365 Z-score: 434.1 bits: 87.9 E(): 1.4e-16; 30.603% identity in 232 aa overlap. Contains Pfam match to entry PF00111 fer2, 2Fe-2S iron-sulfur cluster binding domains 4 2 14 22 [Reporter]SCO1004 (1H15)_PCR/3835 [Gene]SCO1004/6971 NC_003888 2SCG2.17c, unknown, len: 266 aa; similar to part of TR:CAC01640 (EMBL:AL391072) Streptomyces coelicolor conserved hypothetical protein SC9A4.13, 267 aa; fasta scores opt: 191 z-score: 213.7 E(): 0.00022; 52.5% identity in 59 aa overlap. Contains a TTA leucine codon, possible target for bldA regulation 4 2 14 21 [Reporter]SCO5471 (2D15)_PCR/3834 [Gene]SCO5471/6969 NC_003888 SC2A11.05c, gcvH, probable glycine cleavage system H protein, len: 125 aa; highly similar to e.g. GCSH_ECOLI glycine cleavage system H protein (129 aa), fasta scores; opt: 460 z-score: 754.1 E(): 0, 56.0% identity in 125 aa overlap. Contains PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site 4 2 14 20 [Reporter]SCO5922 (3P11)_PCR/3833 [Gene]SCO5922/6967 NC_003888 SC10A5.27c, possible oxidoreductase, len: 326 aa; some similarity to many members of the SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY (SDR) eg. YGFF_ECOLI P52037 hypothet ical oxidoreductase (247 aa), fasta scores; opt: 96 z-score : 310.6 E(): 4.2e-10, 29.1% identity in 213 aa overlap. Con tains PS00017 ATP/GTP-binding site motif A (P-loop) 4 2 14 19 [Reporter]SCO7022 (4L11)_PCR/3832 [Gene]SCO7022/6965 NC_003888 SC1H10.11, unknown, len: 310 aa. 4 2 14 18 [Reporter]SCO5457 (5H11)_PCR/3831 [Gene]SCO5457/6963 NC_003888 SC3D11.14, possible lipoprotein, len: 416aa; similar to many eg. TR:O53223 (EMBL:AL021185) hypothetical protein from Mycobacterium tuberculosis (408 aa) fasta scores; opt: 737, z-score: 800.7, E(): 0, 32.9% identity in 413 aa overlap. Also similar to neighbouring CDS SC3D11.15 fasta scores; opt: 1348, z-score: 1271.2, E(): 0, 53.3% identity in 394 aa overlap. Contains possible N-terminal signal sequence and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site 4 2 14 17 [Reporter]SCO5798 (6D11)_PCR/3830 [Gene]SCO5798/6961 NC_003888 SC4H2.19c, probable secreted protein, len: 409 aa; Contains probable N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Highly similar to downstream CDS SC4H2.18c (405 aa), fasta scores; E(): 0, 61.8% identity in 406 aa overlap 4 2 14 16 [Reporter]SCO6765 (7P7)_PCR/3829 [Gene]SCO6765/6959 NC_003888 SC6A5.14, possible lipoprotein, len: 213 aa; similar to SW:TYRT_STRLN proposed tyrosinase co-factor from the melanin biosynthesis in Streptomyces lincolnensis (140 aa) fasta scores; opt: 115, z-score: 142.8, E(): 1.2, (29.2% identity in 120 aa overlap). Contains Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 4 2 14 15 [Reporter]SCO2475 (8L7)_PCR/3828 [Gene]SCO2475/6957 NC_003888 SC7A8.14, probable lysR-family transcriptional regulator, len: 309 aa; similar to TR:O06466 (EMBL:U94336) Xanthomonas campestris oxidative stress transcriptional regulator OxyR, 313 aa; fasta scores: opt: 374 z-score: 419.6 E(): 5.5e-16; 29.7% identity in 316 aa overlap and SW:OXYR_MYCLE (EMBL:L01095) Mycobacterium leprae probable hydrogen peroxide-inducible genes activator OxyR, 311 aa; fasta scores: opt: 375 z-score: 420.8 E(): 4.8e-16; 33.7% identity in 246 aa overlap. Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family and match to Prosite entry PS00044 Bacterial regulatory proteins, lysR family signature. Contains a possible helix-turn-helix motif at residues 16..37 (+3.82 SD) 4 2 14 14 [Reporter]SCO7747 (9H7)_PCR/3827 [Gene]SCO7747/6955 NC_003888 SC8D11.38, hypothetical protein, len: 392 aa; similar to TR:Q9L195 (EMBL:AL158057) Streptomyces coelicolor hypothetical 47.1 kDa protein SC10G8.07c, 437 aa; fasta scores: opt: 419 z-score: 494.1 E(): 5.5e-20; 28.5% identity in 393 aa overlap 4 2 14 13 [Reporter]SCO6216 (10D7)_PCR/3826 [Gene]SCO6216/6953 NC_003888 SC9G1.06c, hypothetical protein, len: 140 aa; similar to SW:YBAW_ECOLI (EMBL;AE000150) Escherichia coli hypothetical 15.1 KD protein, 132 aa; fasta scores: opt: 165 z-score: 225.5 E(): 3.5e-05; 26.0% identity in 127 aa overlap 4 2 14 12 [Reporter]SCO2370 (11P3)_PCR/3825 [Gene]SCO2370/6951 NC_003888 SCC8A.28c, unknown, len: 159 aa; similar to others from mycobacteria e.g. SW:Q10521 (YM39_MYCTU) hypothetical protein from Mycobacterium tuberculosis (158 aa) fasta scores; opt: 447, z-score: 518.6, E(): 2e-21, 50.4% identity in 141 aa overlap 3 1 19 16 [Reporter]SCO6177 (7C16)_PCR/1419 [Gene]SCO6177/2599 NC_003888 SC6C5.13c, unknown, len: 327aa 4 2 14 11 [Reporter]SCO4127 (12L3)_PCR/3824 [Gene]SCO4127/6949 NC_003888 SCD72A.13c, possible ATP/GTP-binding protein, len: 471 aa; similar to TR:CAB72383 (EMBL:AL138977) putative ATP-binding protein from Streptomyces coelicolor (507 aa) fasta scores; opt: 618, z-score: 740.6, E(): 0, 32.9% identity in 434 aa overlap. Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) 4 2 14 10 [Reporter]SCO3195 (13H3)_PCR/3823 [Gene]SCO3195/6947 NC_003888 SCE22.12, unknown, len: 226 aa 4 2 14 9 [Reporter]SCO3775 (14D3)_PCR/3822 [Gene]SCO3775/6945 NC_003888 SCH63.22c, unknown, len: 577 aa. High content in glycine and alanine amino acid residues 4 2 14 7 [Reporter]SCO3586 (15L23)_PCR/3820 [Gene]SCO3586/6943 NC_003888 SCH66.07c, possible integral membrane protein, len: 330aa; contains a possible membrane spanning hydrophobic region 4 2 14 6 [Reporter]SCO3407 (16H23)_PCR/3819 [Gene]SCO3407/6941 NC_003888 SCE9.14c, conserved hypothetical protein, len: 375 aa; unknown function, highly similar to TR:O06381 (EMBL:Z95436) Mycobacterium tuberculosis hypothetical protein (350 aa), fasta scores; opt: 720 z-score: 820.9 E(): 0, 41.6% identity in 361 aa overlap and similar to TR:O53341 (EMBL:AL021646) Mycobacterium tuberculosis hypothetical protein (472 aa) (25.6% identity in 383 aa overlap) and TR:Q49746 (EMBL:U00016) Mycobacterium leprae hypothetical protein (479 aa) (24.9% identity in 305 aa overlap) 3 1 19 11 [Reporter]SCO4640 (12O8)_PCR/1414 [Gene]SCO4640/2589 NC_003888 SCD82.11, possible tetR-family transcriptional regulator, len: 197 aa; similar to TR:AAF11696 (EMBL:AE002049) Deinococcus radiodurans transcriptional regulator, tetR family DR2148, 204 aa; fasta scores: opt: 175 z-score: 223.6 E(): 4.9e-05; 26.2% identity in 172 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family 4 2 14 5 [Reporter]SCO3131 (17D23)_PCR/3818 [Gene]SCO3131/6939 NC_003888 SCE66.10c, unknown, len: 125aa; similar to TR:Q9RY42 (EMBL:AE001873) hypothetical protein from Deinococcus radiodurans 4 2 14 4 [Reporter]SCO6105 (18P19)_PCR/3817 [Gene]SCO6105/6937 NC_003888 SCBAC1A6.29c, possible regulatory protein, len: 429 aa; N-terminal region similar to N-terminal region of TR:Q9RJZ7 (EMBL:AL133210) Streptomyces coelicolor putative transcriptional regulator SCF37.04, 419 aa; fasta scores: opt: 520 z-score: 594.4 E(): 1.6e-25; 40.8% identity in 240 aa overlap 4 2 14 3 [Reporter]SCP1.92 (19L19)_PCR/3816 [Gene]SCP1.92/6935 NC_003888 SCP1.92, unknown, len: 202aa; 4 2 14 2 [Reporter]SCO5966 (20D19)_PCR/3815 [Gene]SCO5966/6933 NC_003888 SCBAC16H6.01c, possible oxidase, len: >1032aa: similar to many of undefined function eg. TR:Q9EZK2 (EMBL:AF289509) putative oxidase YdiJ-like protein from Pseudomonas putida (1006 aa) fasta scores; opt: 880, Z-score: 889.5, 27.930% identity (31.672% ungapped) in 1024 aa overlap and TR:Q9F2Y0 (EMBL:AL392149) putative oxidoreductase SCD19.22 from Streptomyces coelicolor (951 aa) fasta scores; opt: 622, Z-score: 628.7, 49.698% identity (53.813% ungapped) in 994 aa overlap. Contains Prosite match to PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature; Pfam match to entry PF02913 FAD-oxidase_C, FAD linked oxidases, C-terminal domain and Pfam match to entry PF01565 FAD_binding_4, FAD binding domain.,SC7H1.36c, unknown, partial CDS, len: >81 aa 4 2 13 22 [Reporter]SCO0996 (1D15)_PCR/3813 [Gene]SCO0996/6931 NC_003888 2SCG2.09, possible lipoprotein, len: 347 aa; similar to TR:Q9K467 (EMBL:AL359215) Streptomyces coelicolor putative iron transport lipoprotein SC2H12.17, 345 aa; fasta scores: opt: 473 z-score: 540.7 E(): 1.4e-22; 39.2% identity in 339 aa overlap. Contains Pfam match to entry PF01497 Peripla_BP_2, Periplasmic binding protein, correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 3 1 19 6 [Reporter]SCO5027 (17K4)_PCR/1409 [Gene]SCO5027/2579 NC_003888 SCK15.29c, hypothetical protein, len: 397 aa; similar to TR:Q9RKN1 (EMBL:AL121850) Streptomyces coelicolor hypothetical 37.4 kD protein (fragment) SCC78.05, 347 aa; fasta scores: opt: 180 z-score: 199.8 E(): 0.0012; 28.6% identity in 360 aa overlap 1 4 2 21 [Reporter]SCO4915 (2A1)_PCR/6089 [Gene]SCO4915/11091 NC_003888 SCK13.07, possible integral membrane protein, len: 234 aa. Contains 3 possible hydrophobic membrane spanning regions 4 2 13 21 [Reporter]SCO2388 (2P11)_PCR/3812 [Gene]SCO2388/6929 NC_003888 SC4A7.16, fabH, 3-oxoacyl-[acyl-carrier-protein] synthase III, len: 343 aa; identical to previously sequenced TR:P72392 (EMBL:X86475) Streptomyces coelicolor beta-ketoacyl-acyl carrier protein synthase III FabH, 343 aa and similar to SW:FABH_MYCTU (EMBL:Z95558) Mycobacterium tuberculosis 3-oxoacyl-[acyl-carrier-protein] synthase III (EC 2.3.1.41) (KAS III) FabH, 335 aa; fasta scores: opt: 659 z-score: 720.9 E(): 9e-33; 50.1% identity in 343 aa overlap and to S. coelicolor SC3F7.08, 335 aa; fasta scores: opt: 707 z-score: 718.9 E(): 1.2e-34; 39.5% identity in 332 aa overlap 4 2 13 20 [Reporter]SCO5915 (3L11)_PCR/3811 [Gene]SCO5915/6927 NC_003888 SC10A5.20, unknown, len: 171 aa; weak similarity to part of TR:P79078 (EMBL:Y10421) DELTA-9 FATTY ACID DESATURA SE (EC 1.14.99.5) from CRYPTOCOCCUS CURVATUS (555 aa), fast a scores; opt: 83 z-score: 221.8 E(): 3.7e-05, 25.1% identi ty in 171 aa overlap 4 2 13 19 [Reporter]SCO6022 (4H11)_PCR/3810 [Gene]SCO6022/6925 NC_003888 SC1C3.10, unknown, len: 540 aa; some similarity to hypothetical proteins e.g. M. tuberculosis TR:O06628 (EMBL:Z95618) MTCY07H7A.03 (532 aa), fasta scores; opt: 151 z-score: 278.3 E(): 2.8e-08, 25.9% identity in 529 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature, though this may be spurious. Also similar to flanking genes SC1C3.11 (E(): 2.5e-20, 29.0% identity in 583 aa overlap) and SC1C3.09 (E(): 1.1e-14, 28.0% identity in 542 aa overlap) 4 2 13 18 [Reporter]SCO5452 (5D11)_PCR/3809 [Gene]SCO5452/6923 NC_003888 SC3D11.09, probable ABC transporter, ATP-binding subunit, len: 312aa; similar to many eg. TR:Q9XAC8 (EMBL:AL096839) putative ABC transporter, ATP-binding subunit from Streptomyces coelicolor (307 aa) fasta scores; opt: 713, z-score: 795.0, E(): 0, 43.5% identity in 299 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter, Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. 4 2 13 17 [Reporter]SCO7445 (6P7)_PCR/3808 [Gene]SCO7445/6921 NC_003888 SC5C11.02, unknown, len: 245 aa. Weakly similar to Escherichia coli SW:YJHP_ECOLI(EMBL:U14003) hypothetical 27.4 KD protein (248 aa), fasta scores opt: 301 z-score: 358.4 E(): 1.5e-12 28.2% identity in 248 aa overlap. 3 1 18 22 [Reporter]SCO1133 (1G20)_PCR/1403 [Gene]SCO1133/2569 NC_003888 2SCG38.26c, probable oxidoreductase, molybdopterin binding subunit, len: 330 aa; similar to TR:AAG05320 (EMBL:AE004619) Pseudomonas aeruginosa probable hydroxylase molybdopterin-containing subunit PA1932, 329 aa; fasta scores: opt: 1032 z-score: 1156.3 E(): 0; 52.0% identity in 325 aa overlap and to TR:Q9RK26 (EMBL:AL132856) Streptomyces coelicolor possible oxidoreductase, molybdopterin binding subunit SCF15.11, 330 aa; fasta scores: opt: 1254 z-score: 1403.4 E(): 0; 62.0% identity in 326 aa overlap. Contains Pfam match to entry PF00941 dehydrog_molyb, Molybdopterin binding domain in dehydrogenase 4 2 13 16 [Reporter]SCO6761 (7L7)_PCR/3807 [Gene]SCO6761/6919 NC_003888 SC6A5.10, unknown, len: 52 aa; designated based upon Hidden-Markov Model and probable ribosome binding site. 4 2 13 15 [Reporter]SCO4767 (8H7)_PCR/3806 [Gene]SCO4767/6917 NC_003888 SC6G4.45c, probable regulatory protein, len: 112 aa; similar to TR:Q53963 (EMBL:X62287) S.coelicolor regulatory protein WhiB (87 aa), fasta scores; opt: 201 z-score: 352. 1 E(): 2.3e-12, 49.3% identity in 71 aa overlap 4 2 13 14 [Reporter]SCO7739 (9D7)_PCR/3805 [Gene]SCO7739/6915 NC_003888 SC8D11.30c, possible hydrolase, len: 235 aa; similar to C-terminal region of SW:PCD_ARTOX (EMBL:M94965) Arthrobacter oxidans phemedipham hydrolase (EC 3.1.1.-) Pcd, 493 aa; fasta scores: opt: 219 z-score: 259.2 E(): 6.7e-07; 28.1% identity in 199 aa overlap. Contains 2x Pfam matches to entry PF00135 COesterase, Carboxylesterases 4 2 13 13 [Reporter]SCO6265 (10P3)_PCR/3804 [Gene]SCO6265/6913 NC_003888 SCAH10.30c, scbR, gamma-butyrolactone binding protein, len: 215 aa; identical to previously sequenced TR:O86852 (EMBL:AJ007731) Streptomyces coelicolor gamma-butyrolactone binding protein, 215 aa and to TR:O24741 (EMBL:AB001683) Streptomyces sp. FarA, 221 aa; fasta scores: opt: 771 z-score: 937.4 E(): 0; 57.2% identity in 208 aa overlap. Contains match to Pfam entry PF00440 tetR, Bacterial regulatory proteins, tetR family and to Prosite entry PS01081 Bacterial regulatory proteins, tetR family signature. Contains also a possible helix-turn-helix motif at residues 30..51 (+3.66 SD) 4 2 13 12 [Reporter]SCO2364 (11L3)_PCR/3803 [Gene]SCO2364/6911 NC_003888 SCC8A.22, unknown, len: 331 aa; regions similar to many of undefined function eg. TR:Q9ZBZ1 (EMBL:AL034355) hypothetical protein from Streptomyces coelicolor (482 aa) fasta scores; opt: 686, z-score: 521.2, E(): 1.4e-21, 51.0% identity in 247 aa overlap and SW:P18782 (TERE_ALCSP) tellurium resistance protein from plasmid IncHI2 pMER610 of Alcaligenes sp. (191 aa) fasta scores; opt: 339, z-score: 271.3, E(): 1.2e-07, 35.9% identity in 195 aa overlap 3 1 18 17 [Reporter]SCO7778 (6C16)_PCR/1398 [Gene]SCO7778/2559 NC_003888 SC5E9.26, possible transcriptional regulator, len: 255 aa; similar to many e.g. SW:P15360 (GYLR_STRCO) glycerol operon regulatory protein from Streptomyces coelicolor (254 aa) fasta scores; opt: 217, z-score: 260.0, E(): 5.1e-07, 28.1% identity in 249 aa overlap and TR:O88025 (EMBL:AL031107) putative transcriptional regulator from Streptomyces coelicolor (264 aa) fasta scores; opt: 462, z-score: 543.0, E(): 9e-23, 40.5% identity in 242 aa overlap. Contains Pfam match to entry PF01614 IclR, Bacterial transcriptional regulator. 4 2 13 11 [Reporter]SCO4782 (12H3)_PCR/3802 [Gene]SCO4782/6909 NC_003888 SCD63.14c, possible integral membrane protein, len: 219 aa. Contains possible hydrophobic membrane spanning regions 4 2 13 10 [Reporter]SCO3190 (13D3)_PCR/3801 [Gene]SCO3190/6907 NC_003888 SCE22.07, probable mechanosensitive channel, len: 156 aa; similar to TR:Q9Z5G4 (EMBL:AL035500) Mycobacterium leprae putative gated mechanosensitive ion channel MscL, 154 aa; fasta scores: opt: 248 z-score: 308.9 E(): 9.6e-10; 38.1% identity in 147 aa overlap and to SW:MSCL_CLOPE (EMBL:D50309) Clostridium perfringens large-conductance mechanosensitive channel MscL, 152 aa; fasta scores: opt: 216 z-score: 271.1 E(): 1.2e-07; 34.7% identity in 124 aa overlap. Contains Pfam match to entry PF01741 MscL, Large-conductance mechanosensitive channel, MscL and match to Prosite entry PS01327 Large-conductance mechanosensitive channels mscL family signature. Contains also possible hydrophobic membrane spanning regions 4 2 13 9 [Reporter]SCO0431 (13P23)_PCR/3800 [Gene]SCO0431/6905 NC_003888 SCF51A.09c, possible secreted protein, len: 442 aa; similar to TR:Q9KZR1 (EMBL:AL353832) Streptomyces coelicolor putative secreted protein SCE6.29, 454 aa; fasta scores: opt: 614 Z-score: 655.9 E(): 6.7e-29; 31.278% identity in 454 aa overlap. Contains a possible N-terminal signal sequence 4 2 13 8 [Reporter]SCO1787 (14L23)_PCR/3799 [Gene]SCO1787/6903 NC_003888 SCI51.27c, probable iron-siderophore uptake system transmembrane component, len: 348 aa; similar to many transmembrane components from binding-protein-dependent iron uptake systems using a range of siderophores e.g. SW:FEPD_ECOLI (EMBL:X57471), fepD, Escherichia coli ferric enterobactin transport protein (334 aa), fasta scores; opt: 910 z-score: 991.5 E(): 0, 46.0% identity in 326 aa overlap. Similar to neighbouring SCI51.26c, probable iron-siderophore uptake system transmembrane component (375 aa) (37.9% identity in 348 aa overlap). Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF01032 FecCD_family, FecCD transport family 4 2 13 7 [Reporter]SCO3497 (15H23)_PCR/3798 [Gene]SCO3497/6901 NC_003888 SCE65.33c, probable polyguluronate lyase precursor, len: 259 aa; highly similar to TR:BAA83339 (EMBL:AB030481) Corynebacterium sp. ALY-1 polyguluronate lyase precursor (EC 4.2.2.11) AlyPG, 256 aa; fasta scores: opt: 1329 z-score: 1518.4 E(): 0; 76.9% identity in 260 aa overlap. Contains possible N-terminal signal peptide sequence 3 1 18 12 [Reporter]SCO4400 (11O8)_PCR/1393 [Gene]SCO4400/2549 NC_003888 SCD10.32, possible membrane protein, len: 531 aa. Contains possible hydrophobic membrane spanning region and high content in alanine, glycine and proline amino acid residues. Also high G+C content (79.03%) and a 3x degenerate repeat unit: GGR 1 3 2 13 [Reporter]SCO7141 (9A18)_PCR/4066 [Gene]SCO7141/7397 NC_003888 SC9A4.03, possible nitroreductase, len: 202 aa; similar to SW:NFNB_ECOLI (EMBL:D25414) Escherichia coli oxygen-insensitive NAD(P)H nitroreductase (dihydropteridine reductase) (EC 1.6.99.7) NfnB, 217 aa; fasta scores: opt: 136 z-score: 170.8 E(): 0.048; 28.4% identity in 169 aa overlap. Contains Pfam match to entry PF00881 Nitroreductase, Nitroreductase family 1 3 2 12 [Reporter]SCO2329 (10M14)_PCR/4065 [Gene]SCO2329/7395 NC_003888 SCC53.20c, possible transcriptional regulator, len: 320aa; similar to many eg. TR:Q9S166 (EMBL:AB023785) AdpA transcriptional activator for StrR from Streptomyces griseus (405 aa) fasta scores; opt: 502, z-score: 592.7, E(): 1.5e-25, 32.6% identity in 319 aa overlap and TR:Q9X7Q2 (EMBL:AL049587) putative transcriptional regulator from Streptomyces coelicolor (325 aa) fasta scores; opt: 772, z-score: 909.9, E(): 0, 47.5% identity in 318 aa overlap. Contains Pfam match to entry PF00165 HTH_AraC, Bacterial regulatory helix-turn-helix proteins, araC family, Pfam match to entry PF01965 ThiJ, ThiJ/PfpI family and Prosite match to PS00041 Bacterial regulatory proteins, araC family signature. 1 3 2 11 [Reporter]SCO4496 (11I14)_PCR/4064 [Gene]SCO4496/7393 NC_003888 SCD35.03, possible acetyltransferase, len: 174 aa; similar to TR:CAB71250 (EMBL:AL138598) Streptomyces coelicolor putative acetyltransferase SC1A2.12, 181 aa; fasta scores: opt: 214 z-score: 278.1 E(): 4.6e-08; 32.9% identity in 149 aa overlap and to SW:TTR_PSESY (EMBL:X17150) Pseudomonas syringae (pv. syringae) acetyltransferase (EC 2.3.1.-) Ttr, 17 aa; fasta scores: opt: 137 z-score: 182.0 E(): 0.01; 28.3% identity in 138 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 1 3 2 10 [Reporter]SCO4244 (12E14)_PCR/4063 [Gene]SCO4244/7391 NC_003888 SCD8A.17c, unknown, len: 652 aa 1 3 2 9 [Reporter]SCO1528 (13A14)_PCR/4062 [Gene]SCO1528/7389 NC_003888 SCL2.18, possible elongation factor, len: 732 aa; similar to TR:O07170 (EMBL:Z96071) Mycobacterium tuberculosis FusA2, 714 aa; fasta scores: opt: 2328 z-score: 2349.0 E(): 0; 61.4% identity in 731 aa overlap, to SW:EFG (EMBL:X00415) Escherichia coli elongation factor G (EF-G) FusA, 703 aa; fasta scores: opt: 853 z-score: 862.2 E(): 0; 30.1% identity in 722 aa overlap and to SW:EFG2_STRCO (EMBL:AL031013) Streptomyces coelicolor elongation factor G 2 (EF-G 2), FusB, 686 aa; fasta scores: opt: 937 z-score: 947.0 E(): 0; 35.5% identity in 710 aa overlap. Contains Pfam matches to entries PF00009 GTP_EFTU, Elongation factor Tu family and PF00679 EFG_C, Elongation factor G C-terminus and match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 1 3 2 8 [Reporter]SCO0331 (14M10)_PCR/4061 [Gene]SCO0331/7387 NC_003888 SCF12.10, possible short chain oxidoreductase (putative secreted protein), len: 299 aa; similar to many e.g. TR:CAB51136 (EMBL:Y18817) short chain oxidoreductase from actinorhodin biosynthesis cluster of Streptomyces coelicolor (276 aa) fasta scores; opt: 358, z-score: 403.3, E(): 4.1e-15, 34.6% identity in 280 aa overlap. Contains Pfam match to entry PF00106 adh_short, short chain dehydrogenase 1 3 2 7 [Reporter]SCO3825 (15I10)_PCR/4060 [Gene]SCO3825/7385 NC_003888 SCGD3.26, probable ABC-transporter transmembrane component, len: 282 aa; may be involved in antibiotic resistance by acting as an efflux pump, highly similar to to many ABC-tranporter transmembrane components (especially those involved in antibiotic transport) e.g. TR:O86917 (EMBL:Y15759) Streptomyces rochei ABC-tranporter which induces multidrug resistance (284 aa), fasta scores; opt: 1491 z-score: 1785.3 E(): 0, 77.5% identity in 284 aa overlap. Similar to many from S.coelicolor e.g. (EMBL:AL049841), SCE9.24c, S.coelicolor probable ABC transporter transmembrane component (260 aa) (35.0% identity in 254 aa overlap). Contains hydrophobic, probable membrane-spanning regions. Contains PS00890 ABC-2 type transport system integral membrane proteins signature 1 3 2 6 [Reporter]SCO0373 (16E10)_PCR/4059 [Gene]SCO0373/7383 NC_003888 SCF41.32c, possible secreted protein, len: 199 aa. Contains a possible N-terminal signal sequence. 1 3 2 5 [Reporter]SCO1472 (17A10)_PCR/4058 [Gene]SCO1472/7381 NC_003888 SCL6.29c, conserved hypothetical Sun-family protein, len: 475 aa; similar to SW:SUN_BACSU (EMBL:Y13937) Bacillus subtilis sun protein, 447 aa; fasta scores: opt: 637 z-score: 711.7 E(): 3.2e-32; 32.0% identity in 472 aa overlap. Contains Pfam match to entry PF01029 NusB, NusB family and to entry PF01189 Nol1_Nop2_Sun, NOL1/NOP2/sun family and match to Prosite entry PS01153 NOL1/NOP2/sun family signature 4 3 13 12 [Reporter]SCO2356 (11L2)_PCR/5819 [Gene]SCO2356/10599 NC_003888 SCC8A.14, possible membrane protein, len: 467aa; contains a possible membrane-spanning hydrophobic region close to the N-terminus. 4 3 13 11 [Reporter]SCO4771 (12H2)_PCR/5818 [Gene]SCO4771/10597 NC_003888 SCD63.03, possible inosine-5'-monophosphate dehydrogenase, len: 374 aa; N-terminal region identical to previously sequenced TR:O86845 (EMBL:AJ010601) Streptomyces coelicolor hypothetical 38.9 kD protein (fragment), 84 aa and whole CDS similar to SW:IMDH_ECOLI (EMBL:X02209) Escherichia coli inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) GuaB, 488 aa; fasta scores: opt: 275 z-score: 311.9 E(): 6.1e-10; 29.0% identity in 376 aa overlap. Contains Pfam matches to entries PF01574 IMPDH_N, IMP dehydrogenase / GMP reductase N terminus PF00478 IMPDH_C, IMP dehydrogenase / GMP reductase C terminus 4 3 13 10 [Reporter]SCO2862 (13D2)_PCR/5817 [Gene]SCO2862/10595 NC_003888 SCE20.36c, unknown, len: 77 aa. 4 3 13 9 [Reporter]SCO3215 (13P22)_PCR/5816 [Gene]SCO3215/10593 NC_003888 SCE8.08c, unknown, len: 338 aa 4 3 13 8 [Reporter]SCO3756 (14L22)_PCR/5815 [Gene]SCO3756/10591 NC_003888 SCH63.03c, possible two component system response regulator, len: 224 aa; similar to TR:Q9X8Q7 (EMBL:AL049754) Streptomyces coelicolor putative two-component system regulator SCH10.18c, 228 aa; fasta scores: opt: 827 z-score: 946.4 E(): 0; 62.1% identity in 219 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature 4 3 13 7 [Reporter]SCO0167 (15H22)_PCR/5814 [Gene]SCO0167/10589 NC_003888 SCJ1.16c, unknown, len: 294 aa, similar to many including: Mycobacterium tuberculosis TR:O06189 (EMBL; Z95387)hypothetical 31.7 KD protein MTCY1A10.09C (297 aa), fasta scores opt: 407 z-score: 484.5 E(): 1.3e-19 32.7% identity in 303 aa overlap and Mycobacterium tuberculosis SW:YW12_MYCTU (EMBL; Z74025) hypothetical 30.9 KD protein MTCY39.12 (295 aa), fasta scores opt: 401 z-score: 477.5 E(): 3.1e-19 34.7% identity in 294 aa overlap. Also similar to S. coelicolor SCJ1.29c (283 aa), fasta scores opt: 806 z-score: 761.9 E(): 0 46.4% identity in 295 aa overlap. Contains a Pfam match to entry PF00582 Usp, Universal stress protein family. 4 3 13 6 [Reporter]SCO1028 (16D22)_PCR/5813 [Gene]SCO1028/10587 NC_003888 SCG20A.08c, possible lyase, len: 374 aa; highly similar to TR:O69652 (EMBL:AL022121) Mycobacterium tuberculosis putative lyase MTV025.032, 346 aa; fasta scores: opt: 1469 z-score: 1762.4 E(): 0; 63.3% identity in 341 aa overlap. Contains Pfam match to entry PF00291 S_T_dehydratase, Pyridoxal-phosphate dependent enzyme 4 3 13 3 [Reporter]SCO4989 (19H18)_PCR/5810 [Gene]SCO4989/10585 NC_003888 2SCK36.12, probable IclR-family transcriptional regulatory protein, len: 254 aa; similar to SW:KDGR_ECOLI (EMBL:AE000277) Escherichia coli transcriptional regulator KdgR, 263 aa; fasta scores: opt: 420 Z-score: 488.7 bits: 98.0 E(): 1.3e-19; 30.992% identity in 242 aa overlap. Contains Pfam match to entry PF01614 IclR, Bacterial transcriptional regulator and possible helix-turn-helix motif at residues 20..41 (+3.12 SD) 4 3 13 2 [Reporter]SCO0974 (20P14)_PCR/5809 [Gene]SCO0974/10583 NC_003888 SCBAC19F3.01c, possible secreted protein, len: >237aa: interesting similarity to TR:O86308 (EMBL:Z96935) a resuscitation-promoting factor from Micrococcus luteus (220 aa) fasta scores; opt: 499, Z-score: 540.6, 41.784% identity (45.408% ungapped) in 213 aa overlap. Similar to many others eg. TR:Q9F2Q2 (EMBL:AL442120) putative secreted protein from Streptomyces coelicolor (244 aa) fasta scores; opt: 461, Z-score: 499.7, 44.033% identity (51.442% ungapped) in 243 aa overlap. Contains Pfam match to entry PF01476 LysM, LysM domain and has a possible N-terminal signal sequence.,SCM11.29c, probable secreted protein (partial CDS), len: >234 aa; similar to TR:Q9X7W8 (EMBL:AL049485) Streptomyces coelicolor putative secreted peptidase SC6A5, 312 aa; fast ascores: opt: 684 z-score: 619.7 E(): 3.9e-27; 55.2% identity in 183 aa overlap and to SW:ALE1_STACP (EMBL:D86328) Staphylococcus capitis glycyl-glycine endopeptidase Ale-1 precursor (EC 3.4.24.75), 362 aa; fasta scores: opt: 313 z-score: 291.5 E(): 7.4e-09; 29.3% identity in 229 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 3 12 22 [Reporter]SCO1260 (1P10)_PCR/5807 [Gene]SCO1260/10581 NC_003888 2SCG18.07, probable two-component system response regulator, len: 220 aa; similar to TR:CAB95289 (EMBL:AL359779) Streptomyces coelicolor putative two-component system response regulator SCG22.16c, 221 aa; fasta scores: opt: 756 z-score: 640.4 E(): 4.5e-30; 57.7% identity in 222 aa overlap and to TR:Q9Z677 (EMBL:AF102271) Streptomyces coelicolor response-regulator, 213 aa; fasta scores: opt: 610 z-score: 693.5 E(): 3.8e-31; 50.7% identity in 215 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00196 GerE, Bacterial regulatory proteins, luxR family and match to Prosite entry PS00622 Bacterial regulatory proteins, luxR family signature. Also contains possible helix-turn-helix motif at residues 172..193 (+3.03 SD) 4 3 12 21 [Reporter]SCO3669 (2L10)_PCR/5806 [Gene]SCO3669/10579 NC_003888 SCH44.09c, dnaJ, molecular chaperone, len: 399 aa; identical to previously sequenced SW:DNAJ_STRCO (EMBL:L46700) Streptomyces colicolor DnaJ protein, 399 aa and similar to TR:CAB66232 (EMBL:AL136503) Streptomyces coelicolor SCC77.21c heat shock protein DnaJ2, 378 aa; fasta scores: opt: 713 z-score: 611.9 E(): 1.5e-28; 38.8% identity in 397 aa overlap. Contains Pfam matches to entries PF00226 DnaJ, DnaJ domain, PF00684 DnaJ_CXXCXGXG, DnaJ central domain (4 repeats) and PF01556 DnaJ_C, DnaJ C terminal region and matches to Prosite entries PS00636 Nt-dnaJ domain signature and PS00637 CXXCXGXG dnaJ domain signature 4 3 12 20 [Reporter]SCO1483 (3H10)_PCR/5805 [Gene]SCO1483/10577 NC_003888 SC9C5.07c, pyrA, carbamoylphosphate synthetase large chain, len: 1102 aa; similar to SW:CARB_ECOLI (EMBL:V01500) Escherichia coli carbamoyl-phosphate synthase large chain (EC 6.3.5.5) CarB or PyrA, 1072 aa; fasta scores: opt: 3383 z-score: 3699.6 E(): 0; 55.0% identity in 1102 aa overlap. Contains 3x Pfam matches to entry PF00289 CPSase_L_chain, Carbamoyl-phosphate synthase (CPSase) and matches to Prosite entries 2x PS00866 Carbamoyl-phosphate synthase subdomain signature 1 and 2x PS00867 Carbamoyl-phosphate synthase subdomain signature 4 3 12 19 [Reporter]SCO5550 (4D10)_PCR/5804 [Gene]SCO5550/10575 NC_003888 SC1C2.31c, probable transcriptional regulator, len: 214 aa; N-terminus is similar to the N-terminus of members of the MerR family, e.g. MERR_THIFE mercuric resistance operon regulatory protein (122 aa), fasta scores; opt: 111 z-score: 189.7 E(): 0.0026, 30.3% identity in 66 aa overlap. Contains probable helix-turn-helix motif at aa 2-23 (1799, +5.31 SD) 4 3 12 18 [Reporter]SCO2201 (5P6)_PCR/5803 [Gene]SCO2201/10573 NC_003888 SC3H12.09, conserved hypothetical protein, len: 123 aa; identical to previously sequenced TR:CAB58283 (EMBL:AL121850) Streptomyces coelicolor conserved hypothetical protein SCC78.02, 123 aa and similar to TR:Q9ZBQ5 (EMBL:AL034447) Streptomyces coelicolor hypothetical 17.8 kD protein SC7A1.18c, 141 aa; fasta scores: opt: 317 z-score: 412.3 E(): 1.4e-15; 44.6% identity in 112 aa overlap. Contains Pfam match to entry PF01638 DUF24, Protein of unknown function 4 3 12 17 [Reporter]SCO5823 (6L6)_PCR/5802 [Gene]SCO5823/10571 NC_003888 SC5B8.13, probable integral membrane protein, len: 1 74 aa; contains five possible transmembrane domains 4 3 12 16 [Reporter]SCO7270 (7H6)_PCR/5801 [Gene]SCO7270/10569 NC_003888 SC5H1.22, probable transcriptional regulator, len: 294 aa; similar to many LysR family regulators e.g. SW:BUDR_KLETE (EMBL:Z48600), budR, Klebsiella terrigena bud operon transcriptional regulator (290 aa), fasta scores; opt: 392 z-score: 459.0 E(): 3.1e-18, 33.7% identity in 267 aa overlap. Similar to TR:O86805 (EMBL:AL031317) S.coelicolor probable transcriptional regulator (306 aa) (29.3% identity in 304 aa overlap). Contains probable helix-turn-helix motif at aa 17-38 (Score 1145, +3.09 SD). Contains Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix proteins, lysR family, score 171.10, E-value 1.9e-47 and PS00044 Bacterial regulatory proteins, lysR family signature 4 3 12 15 [Reporter]SCO4751 (8D6)_PCR/5800 [Gene]SCO4751/10567 NC_003888 SC6G4.29, probable acetyltransferase, len: 177 aa; s imilar to e.g. RIMI_ECOLI ribosomal-protein-alanine acetyl transferase (148 aa), fasta scores; opt: 166 z-score: 257.4 E(): 4.5e-07, 33.3% identity in 120 aa overlap. Contains Pf am match to entry PF00583 Acetyltransf, Acetyltransferase ( GNAT) family, score 59.60, E-value 6.7e-14 4 3 12 14 [Reporter]SCO2655 (9P2)_PCR/5799 [Gene]SCO2655/10565 NC_003888 SC8E4A.25c, hypothetical protein, len: 178 aa; similar to various hypothetical proteins, e.g. TR:Q9X7B4 (EMBL:AL049913) Mycobacterium leprae hypothetical 23.9 kD protein MLCB1610.15, 215 aa; fasta scores: opt: 589 z-score: 757.6 E(): 0; 52.8% identity in 161 aa overlap. Contains Pfam match to entry PF01844 HNH, HNH endonuclease 4 3 12 13 [Reporter]SCO6258 (10L2)_PCR/5798 [Gene]SCO6258/10563 NC_003888 SCAH10.23, probable ABC transport system sugar permease, len: 346 aa; similar to TR:O68120 (EMBL:AF010496) Rhodobacter capsulatus ribose transport system permease protein RbsC; fasta scores: opt: 445 z-score: 508.0 E(): 6.4e-21; 30.5% identity in 351 aa overlap and to SW:RBSC_ECOLI (EMBL:L10328) Escherichia coli ribose transport system permease protein RbsC, len: 321 aa; fasta scores: opt: 286 z-score: 329.9 E(): 5.3e-11; 30.1% identity in 329 aa overlap. Contains possible hydrophobic membrane spanning regions 4 3 12 12 [Reporter]SCO2257 (11H2)_PCR/5797 [Gene]SCO2257/10561 NC_003888 SCC75A.03, probable ABC transporter, ATP-binding component, len: 365 aa. Highly similar to many ABC transport proteins e.g. Streptomyces peucetius SW:DRRA_STRPE(EMBL:M73758) daunorubicin resistance ATP-binding protein (330 aa), fasta scores opt: 922 z-score: 1009.0 E():0 48.9% identity in 329 aa overlap and Streptomyces antibioticus TR:Q53716(EMBL:L06249) ATP-binding protein OLEC-ORF4 involved in oleandomycin-resistance (325 aa), fasta scores opt: 1883 z-score: 2052.8 E():0 89.7% identity in 329 aa overlap. Also similar to several Streptomyces coelicolor putative ABC transporter ATP-binding components e.g TR:CAB42771(EMBL:AL049841) SCE9.25C (315 aa), fasta scores opt: 1031 z-score: 1127.7 E():0 54.4% identity in 318 aa overlap. Contains Prosite hits to PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Also contains a Pfam match to entry PF00005 ABC_tran, ABC transporter. 4 3 12 11 [Reporter]SCO4456 (12D2)_PCR/5796 [Gene]SCO4456/10559 NC_003888 SCD6.34, possible membrane-associated oxidoreductase, len: 528 aa; similar to TR:CAB52970 (EMBL:AL109950) Streptomyces coelicolor putative membrane-associated oxidoreductase SCJ4.36c, 503 aa; fasta scores: opt: 657 z-score: 776.7 E(): 0; 38.8% identity in 551 aa overlap. Contains possible C-terminal region hydrophobic membrane spanning domains 4 3 12 9 [Reporter]SCO5021 (13L22)_PCR/5794 [Gene]SCO5021/10557 NC_003888 SCK15.23, possible lipoprotein, len: 221 aa; similar to Streptomyces coelicolor SCK15.24, 220 aa; fasta scores: opt: 736 z-score: 652.9 E(): 8.3e-31; 54.3% identity in 221 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 3 12 8 [Reporter]SCO0161 (14H22)_PCR/5793 [Gene]SCO0161/10555 NC_003888 SCJ1.10c, doubtful CDS, len:124 aa. 4 3 12 7 [Reporter]SCO3421 (15D22)_PCR/5792 [Gene]SCO3421/10553 NC_003888 SCE9.28c, hypothetical protein, len: 274 aa; similar others from S.coelicolor e.g. TR:O86719 (EMBL:AL031515), SC5C7.22C, hypothetical protein (287 aa), fasta scores; opt: 727 z-score: 871.9 E(): 0, 44.7% identity in 273 aa overlap 4 3 12 6 [Reporter]SCO5143 (16P18)_PCR/5791 [Gene]SCO5143/10551 NC_003888 SCP8.06, tag, DNA-3-methyladenine glycosylase I, len: 194 aa; highly similar to SW:3MG1_ECOLI (EMBL:J02606) Escherichia coli DNA-3-methyladenine glycosylase I (EC 3.2.2.20) Tag, 187 aa; fasta scores: opt: 529 z-score: 636.5 E(): 5.5e-28; 45.2% identity in 177 aa overlap 4 3 12 5 [Reporter]SCO3890 (17L18)_PCR/5790 [Gene]SCO3890/10549 NC_003888 SCH24.12c, trxB, thioredoxin reductase (NADPH), len: 322 aa; previously sequenced as SW:TRXB_STRCO (EMBL:AJ007313), TrxB. Contains PS00573 Pyridine nucleotide-disulphide oxidoreductases class-II active site 4 3 12 4 [Reporter]SCO1705 (18H18)_PCR/5789 [Gene]SCO1705/10547 NC_003888 SCI30A.26c, probable alcohol dehydrogenase (zinc-binding), len: 365 aa; similar to many e.g. SW:ADH3_ECOLI alcohol dehydrogenase class III from Escherichia coli (369 aa) fasta scores; opt: 819, z-score: 864.3, E(): 0, (38.6% identity in 368 aa overlap). Contains Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and Prosite matche to PS00059 Zinc-containing alcohol dehydrogenases signature. 4 3 12 3 [Reporter]SCO1307 (19D18)_PCR/5788 [Gene]SCO1307/10545 NC_003888 SCBAC36F5.18c, unknown, len: 468 aa 4 3 12 2 [Reporter]SCO2814 (20L14)_PCR/5787 [Gene]SCO2814/10543 NC_003888 SCBAC17F8.05c, possible methyltransferase, len: 285 aa: similar to many some of which reside within antibiotic biosynthetic clusters e.g. TR:Q54818 (EMBL:L35560) doxorubicin polyketide biosynthesis gene from Streptomyces peucetius (286 aa) fasta scores; opt: 258, Z-score: 287.1, 38.136% identity (39.130% ungapped) in 118 aa overlap, TR:Q9S0N6 (EMBL:AB032524) C5-O-methyltransferase AveD from the avermectin biosynthetic cluster of Streptomyces avermitilis (283 aa) fasta scores; opt: 249, Z-score: 277.5, 30.508% identity (34.123% ungapped) in 236 aa overlap and TR:AAK64748 (EMBL:AE007203) hypothetical protein from Rhizobium meliloti (261 aa) fasta scores; opt: 581, Z-score: 635.1, 41.406% identity (42.570% ungapped) in 256 aa overlap. 4 3 11 22 [Reporter]SCO1256 (1L10)_PCR/5785 [Gene]SCO1256/10541 NC_003888 2SCG18.03c, unknown, len: 146 aa 4 3 11 21 [Reporter]SCO1580 (2H10)_PCR/5784 [Gene]SCO1580/10539 NC_003888 SCI35.02c, argC, N-acetyl-gamma-glutamyl-phosphate reductase, len: 342 aa; identical to ARGC_STRCO N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38) (249 aa). Contains PS01224 N-acetyl-gamma-glutamyl-phosphate reductase active site. Referred to as 'argA' on genetic map. 4 3 11 20 [Reporter]SCO4087 (3D10)_PCR/5783 [Gene]SCO4087/10537 NC_003888 SCD25.23, purM, phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), len: 355 aa. Identical to Streptomyces lividans TR:Q9ZB03 (EMBL:U64826) phosphoribosyl aminoimidazole synthetase (EC 6.3.3.1) (355 aa), fasta scores opt: 2313 z-score: 2645.5 E(): 0; 100.0% identity in 355 aa overlap and highly similar to many others e.g. Escherichia coli SW:PUR5_ECOLI (EMBL:M13747) phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1) (AIRS) (phosphoribosyl-aminoimidazole synthetase) (AIR synthase) (344 aa), fasta scores opt: 1018 z-score: 1167.8 E(): 0; 48.4% identity in 343 aa overlap. Contains a Pfam match to entry PF00586 AIRS, AIR synthase related proteins. 4 3 11 19 [Reporter]SCO2240 (4P6)_PCR/5782 [Gene]SCO2240/10535 NC_003888 SC1G2.02c, possible membrane protein, len: 222 aa. Contains a possible N-terminal membrane spanning hydrophobic domain. 4 3 11 18 [Reporter]SCO2196 (5L6)_PCR/5781 [Gene]SCO2196/10533 NC_003888 SC5F7.05, possible integral membrane protein, len: 234aa; similar to SW:Y00U_MYCTU hypothetical protein from Mycobacterium tuberculosis (250 aa) fasta scores; opt: 574, z-score: 642.0, E(): 2e-28, (39.4% identity in 226 aa overlap). Contains possible membrane spanning hydrophobic regions. 4 3 11 16 [Reporter]SCO7274 (7D6)_PCR/5779 [Gene]SCO7274/10531 NC_003888 SC5H1.18, possible membrane protein, len: 400 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition. Contains hydrophobic, probable membrane-spanning regions 4 3 11 15 [Reporter]SCO5573 (8P2)_PCR/5778 [Gene]SCO5573/10529 NC_003888 SC7A1.17, fpg, formamidopyrimidine-DNA glycosylase, len: 286aa; similar to many eg. TR:O69470 (EMBL:AL023635) putative formamidopyrimidine-DNA glycosylase from Mycobacterium leprae (282 aa) fasta scores; opt: 1075, z-score: 1367.9, E(): 0, (59.8% identity in 286 aa overlap) and SW:FPG_ECOLI formamidopyrimidine-DNA glycosylase from Escherichia coli(269 aa) fasta scores; opt: 508, z-score: 712.6, E(): 2.1e-32, (36.2% identity in 282 aa overlap). Contains Pfam match to entry PF01149 Fapy_DNA_glyco, Formamidopyrimidine-DNA glycosylase, score 390.10, E-value 2.2e-113. 4 3 11 14 [Reporter]SCO2651 (9L2)_PCR/5777 [Gene]SCO2651/10527 NC_003888 SC8E4A.21c, possible dehydrogenase/reductase (putative secreted protein), len: 245 aa; similar to TR:CAB52925 (EMBL:AL109949) Streptomyces coelicolor putative oxidoreductase SCJ11.40, 250 aa; fasta scores: opt: 717 z-score: 778.8 E(): 0; 47.6% identity in 250 aa overlap and to SW:LINX_PSEPA (EMBL:D23722) Pseudomonas paucimobilis 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (EC 1.1.-.-) LinX, 250 aa; fasta scores: opt: 446 z-score: 488.9 E(): 8e-20; 34.3% identity in 248 aa overlap. Contains Pfam matches to entries PF00106 adh_short, short chain dehydrogenase and PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and match to Prosite entry PS00061 Short-chain. Contains possible N-terminal region signal peptide sequence dehydrogenases/reductases family signature 4 3 11 13 [Reporter]SCO6489 (10H2)_PCR/5776 [Gene]SCO6489/10525 NC_003888 SC9C7.25, conserved hypothetical protein, len: 310 aa; similar to several proteins of undefined function egs. TR:P74220 (EMBL:D90913) hypothetical protein from Synechocystis sp. (strain PCC 6803) (301 aa) fasta scores; opt: 642, z-score: 649.7, E(): 6.7e-29, (38.8% identity in 309 aa overlap) and TR:Q47511 (EMBL:X57583) MccF protein from Escherichia coli plasmid pMCCC7 thought to be involved in self-immunity to translation inhibitor microcin C7 (344 aa) fasta scores; opt: 178, z-score: 259.5, E(): 3.6e-07, (25.1% identity in 339 aa overlap) 4 3 11 12 [Reporter]SCO2713 (11D2)_PCR/5775 [Gene]SCO2713/10523 NC_003888 SCC61A.34, unknown, len: 382aa; 4 3 11 11 [Reporter]SCO4428 (11P22)_PCR/5774 [Gene]SCO4428/10521 NC_003888 SCD6.06c, possible secreted protein, len: 507 aa. Contains possible N-terminal region signal peptide sequence 4 3 11 10 [Reporter]SCO3538 (12L22)_PCR/5773 [Gene]SCO3538/10519 NC_003888 SCE2.19c, unknown, len: 247aa; 4 3 11 9 [Reporter]SCO3677 (13H22)_PCR/5772 [Gene]SCO3677/10517 NC_003888 SCH35.47, possible purine phosphoribosyltransferase, len: 165 aa; similar to many eg. TR:P74916 (EMBL:D37928) purine phosphoribosyltransferase from Thermus flavus (154 aa) fasta scores; opt: 222, z-score: 291.9, E(): 6.3e-09, 931.8% identity in 151 aa overlap). 4 3 11 8 [Reporter]SCO0335 (14D22)_PCR/5771 [Gene]SCO0335/10515 NC_003888 SCF12.14, putative secreted protein, len: 1044 aa 4 3 11 7 [Reporter]SCO3771 (15P18)_PCR/5770 [Gene]SCO3771/10513 NC_003888 SCH63.18c, probable secreted penicillin binding protein, len: 546 aa; similar to TR:P72405 (EMBL:U56256) Streptomyces clavuligerus high molecular weight group B penicillin binding protein PcbR, 551 aa; fasta scores: opt: 1063 z-score: 1122.1 E(): 0; 37.7% identity in 559 aa overlap. Contains Pfam match to entry PF00905 Transpeptidase, Penicillin binding protein transpeptidase domain and possible N-terminal region signal peptide sequence 4 3 11 6 [Reporter]SCO1446 (16L18)_PCR/5769 [Gene]SCO1446/10511 NC_003888 SCL6.03, possible integral membrane protein, len: 122 aa. Contains possible hydrophobic membrane spanning regions 4 3 11 5 [Reporter]SCO1196 (17H18)_PCR/5768 [Gene]SCO1196/10509 NC_003888 SCG11A.27c, putative secreted protein, len: 287 aa. Contains possible N-terminal region signal peptide sequence 4 3 11 4 [Reporter]SCO0566 (18D18)_PCR/5767 [Gene]SCO0566/10507 NC_003888 SCF73.13c, probable membrane protein, len: 94 aa. Contains possible hydrophobic membrane spanning region 4 3 11 3 [Reporter]SCO1983 (19P14)_PCR/5766 [Gene]SCO1983/10505 NC_003888 SC3C9.18c, conserved hypothetical protein, len: 229 aa; similar to TR:BAB49166 (EMBL:AP002998) Rhizobium loti MLR1911 protein, 249 aa; fasta scores: opt: 689 Z-score: 839.8 bits: 162.8 E(): 3.6e-39; 50.000% identity in 234 aa overlap. Contains a TTA leucine codon, possible target for bldA regulation 4 3 11 2 [Reporter]SCO3050 (20H14)_PCR/5765 [Gene]SCO3050/10503 NC_003888 SCBAC19G2.05c, unknown, len: 346aa: no significant database matches. 4 3 10 22 [Reporter]SCO2794 (1H10)_PCR/5763 [Gene]SCO2794/10501 NC_003888 2SCC13.02c, lacI-family transcriptional regulatory protein, len: 351 aa; highly similar to TR:Q9X9R3 (EMBL:AJ009798) Streptomyces reticuli CebR protein, 350 aa; fasta scores: opt: 2011 z-score: 2174.4 E(): 0; 89.4% identity in 350 aa overlap and C-terminal region identical to TR:BAB03462 (EMBL:AB036424) Streptomyces coelicolor transcriptional regulator (fragment) ORF5-H, 193 aa. Contains Pfam matches to entries PF00356 lacI, Bacterial regulatory proteins, lacI family and PF00532 Peripla_BP_like, Periplasmic binding proteins and sugar binding domain of the LacI family and match to Prosite entry PS00356 Bacterial regulatory proteins, lacI family signature. Also contains a possible helix-turn-helix motif at residues 14..35 (+6.05 SD) 4 2 13 6 [Reporter]SCO4621 (16D23)_PCR/3797 [Gene]SCO4621/6899 NC_003888 SCD39.21c, traA1, possible sporulation-related protein, len: 179 aa; highly similar to TR:Q54678 (EMBL:U23762) Streptomyces phaeochromogenes involved in intermycelial plasmid transfer TraA, 184 aa; fasta scores: opt: 472 z-score: 523.1 E(): 1.1e-21; 50.0% identity in 172 aa overlap 4 2 13 4 [Reporter]SCO4051 (18L19)_PCR/3795 [Gene]SCO4051/6897 NC_003888 2SCD60.17, conserved hypothetical protein, len: 165 aa; similar to TR:P72845 (EMBL:D90901) Synechocystis sp. hypothetical 18.8 kDa protein SLL1188, 164 aa; fasta scores: opt: 339 z-score: 385.2 E(): 6.8e-14; 38.3% identity in 154 aa overlap 4 2 13 3 [Reporter]SCP1.18c (19H19)_PCR/3794 [Gene]SCP1.18c/6895 NC_003888 SCP1.18c, possible DNA integrase/recombinase, len: 783aa; C-terminal region weakly similar to many members of the 'phage' integrase family eg. SW:P06697 (TNPB_STAAU) transposase B (transposon Tn554) from Staphylococcus aureus (630 aa) fasta scores; opt: 205, z-score: 242.4, E(): 4.9e-06, 22.1% identity in 280 aa overlap. Contains Pfam match to entry PF00589 Phage_integrase, Phage integrase family. 4 2 13 2 [Reporter]SCO3047 (20P15)_PCR/3793 [Gene]SCO3047/6893 NC_003888 SCBAC19G2.02, possible membrane protein, len: 524aa: similar to many eg. neighbouring gene TR:Q9KZK3 (EMBL:AL353862) SCE34.23 putative transcriptional regulator (396 aa) fasta scores; opt: 631, Z-score: 610.7, 36.011% identity (38.235% ungapped) in 361 aa overlap and TR:O68907 (EMBL:AF058302) FrnA from polyketide antibiotic frenolicin biosynthetic cluster of Streptomyces roseofulvus (770 aa) fasta scores; opt: 482, Z-score: 464.5, 28.387% identity (32.195% ungapped) in 465 aa overlap. Contains possible membrane-spanning hydrophobic region. Also has region of similarity with adjacent CDS SCBAC19F3.03c. 4 2 12 22 [Reporter]SCO1268 (1P11)_PCR/3791 [Gene]SCO1268/6891 NC_003888 2SCG18.15c, probable acyltransferase, len: 372 aa; similar to SW:ODO2_ECOLI (EMBL:J01619) Escherichia coli dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61) SucB, 404 aa; fasta scores: opt: 281 z-score: 311.6 E(): 7.1e-10; 24.8% identity in 400 aa overlap. Contains Pfam match to entry PF00364 biotin_lipoyl, Biotin-requiring enzymes and match to Prosite entry PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site 4 2 12 21 [Reporter]SCO7638 (2L11)_PCR/3790 [Gene]SCO7638/6889 NC_003888 SC10F4.11c, eno2, enolase, len: 434 aa; highly similar to SW:ENO_ECOLI (EMBL:X82400) Escherichia coli enolase (EC 4.2.1.11) Eno, 431 aa; fasta scores: opt: 1537 z-score: 1648.0 E(): 0; 57.5% identity in 426 aa overlap and to TR:CAC09537 (EMBL:AL442120) Streptomyces coelicolor eno, enolase SCE41.05c, 426 aa; fasta scores: opt: 1594 z-score: 1484.2 E(): 0; 58.2% identity in 423 aa overlap. Contains Pfam match to entry PF00113 enolase, Enol-ase and match to Prosite entry PS00164 Enolase signature 4 2 12 20 [Reporter]SCO7236 (3H11)_PCR/3789 [Gene]SCO7236/6887 NC_003888 SC7A12.03c, qcrB3, ubiquinol-cytochrome C reductase cytochrome subunit B, len: 554 aa. Highly similar to many including: Streptomyces lividans SW:QCRB_STRLI(EMBL:AF107888) ubiquinol-cytochrome C reductase cytochrome subunit B (549 aa), fasta scores opt: 1808 z-score: 1914.0 E():0 51.9% identity in 528 aa overlap and Streptomyces coelicolor SW:QCRB_STRCO (EMBL:AL049497) ubiquinol-cytochrome C reductase cytochrome subunit B, QcrB OR SC6G10.21c (545 aa), opt: 2016 z-score: 2133.7 E():0 55.0% identity in 522 aa overlap. Contains a Pfam match to entry PF00033 cytochrome_b_N, Cytochrome b(N-terminal)/b6/petB and multiple possible membrane spanning hydrophobic domains. 4 2 12 19 [Reporter]SCO6018 (4D11)_PCR/3788 [Gene]SCO6018/6885 NC_003888 SC1C3.06, unknown, len: 341 aa; similar to downsteam CDS SC1C3.07 (E(): 2.3e-19, 36.2% identity in 348 aa overlap, and to C-terminus of SC1C3.13 (E(): 3.7e-09, 32.0% identity in 356 aa overlap) 4 2 12 17 [Reporter]SCO5831 (6L7)_PCR/3786 [Gene]SCO5831/6883 NC_003888 SC5B8.21c, citrate synthase-like protein, len: 421 a a; similar to citrate synthase from many organisms e.g. CIS Y_THIFE P51045 thiobacillus ferrooxidans. citrate synthase (386 aa), fasta scores; opt: 387 z-score: 460.4 E(): 1.9e-1 8, 30.2% identity in 384 aa overlap. Contains Pfam match to entry citrate_synt PF00285, Citrate synthase, score 81.00 and probable helix-turn-helix at aa 17-38 (Score 1757, +5.1 7 SD). Also similar to upstream gene SC5B8.22 (390 aa) E(): 4.5e-11, 32.8% identity in 351 aa overlap. 4 2 12 16 [Reporter]SCO7261 (7H7)_PCR/3785 [Gene]SCO7261/6881 NC_003888 SC5H1.31, possible membrane protein, len: 147 aa; unknown function, similar to TR:O07432 (EMBL:Z97050) Mycobacterium tuberculosis hypothetical protein (143 aa), fasta scores; opt: 326 z-score: 414.4 E(): 9.2e-16, 36.2% identity in 141 aa overlap 4 2 12 15 [Reporter]SCO4763 (8D7)_PCR/3784 [Gene]SCO4763/6879 NC_003888 SC6G4.41c, unknown, len: 413 aa 4 2 12 14 [Reporter]SCO7811 (9P3)_PCR/3783 [Gene]SCO7811/6877 NC_003888 SC8E7.08c, possible membrane protein, len: 126 aa. Weakly similar to several e.g. Streptomyces coelicolor TR:Q9RJS0(EMBL:AL132707) probable integral membrane protein, SCF51.15C (201 aa), fasta scores opt: 106 z-score: 142.5 E():1.8 33.7% identity in 104 aa overlap. Contains possible membrane spanning hydrophobic domains. 4 2 12 13 [Reporter]SCO6268 (10L3)_PCR/3782 [Gene]SCO6268/6875 NC_003888 SCAH10.33c, possible histidine kinase orfB, len: 254 aa; identical to previously sequenced TR:O86849 (EMBL:AJ007731) Streptomyces coelicolor putative histidine kinase ORFB, 310 aa and to SW:UHPB_SALTY (EMBL:M89480) Salmonella typhimurium sensor protein UhpB, 500 aa; fasta scores: opt: 219 z-score: 254.8 E(): 8e-07; 33.3% identity in 204 aa overlap. Contains a match to Prosite entry PS00136 Serine proteases, subtilase family, aspartic acid active site 4 2 12 12 [Reporter]SCO2265 (11H3)_PCR/3781 [Gene]SCO2265/6873 NC_003888 SCC75A.11c, unknown, len: 92 aa. 4 2 12 10 [Reporter]SCO2840 (12P23)_PCR/3779 [Gene]SCO2840/6871 NC_003888 SCE20.14, possible LysR-family transcriptional regulatory protein, len: 297 aa. Similar to many other regulators including: Bacillus subtilis SW:ALSR_BACSU (EMBL:L04470;) als operon regulatory protein (302 aa). fasta scores opt: 373 z-score: 437.5 E(): 5.5e-17 26.6% identity in 286 aa overlap and Streptomyces lividans TR:Q9X9S5 (EMBL:Y18818) transcriptional regulator of LysR family (307 aa), fasta scores opt: 449 z-score: 525.1 E(): 7.3e-22 34.0% identity in 303 aa overlap. Contains a Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family with a putative helix-turn-helix motif situated between residues 17..38 (+4.47 SD). 4 2 12 9 [Reporter]SCO0585 (13L23)_PCR/3778 [Gene]SCO0585/6869 NC_003888 SCF55.09c, cvnD11, probable ATP/GTP binding protein, len: 178 aa; similar to TR:CAB41559 (EMBL:AL049727) Streptomyces coelicolor putative ATP/GTP binding protein, SC9B1.13c, 181 aa; fasta scores: opt: 617 z-score: 780.0 E(): 0; 57.5% identity in 179 aa overlap and low similarity to SW:YPT1_VOLCA (EMBL:M93438) Volvox carteri GTP binding protein YPTV1, 203 aa; fasta scores: opt: 117 z-score: 156.9 E(): 0.23; 23.2% identity in 138 aa overlap. Contains match to Prosite entry PS00017 ATP/GTP-binding site motif A (P-loop) 4 2 12 8 [Reporter]SCO3663 (14H23)_PCR/3777 [Gene]SCO3663/6867 NC_003888 SCH44.03c, possible membrane protein, len: 63 aa. Contains possible hydrophobic membrane spanning regions 4 2 12 7 [Reporter]SCO4974 (15D23)_PCR/3776 [Gene]SCO4974/6865 NC_003888 2SCK31.34, possible deaminase, len: 167 aa; similar to TR:AAG05887 (EMBL:AE004677) Pseudomonas aeruginosa probable deaminase PA2499, 151 aa; fasta scores: opt: 299 z-score: 372.9 E(): 3.1e-13; 36.6% identity in 123 aa overlap. Contains Pfam match to entry PF00383 dCMP_cyt_deam, Cytidine and deoxycytidylate deaminase zinc-binding region 4 2 12 6 [Reporter]SCO0230 (16P19)_PCR/3775 [Gene]SCO0230/6863 NC_003888 SCJ9A.09, possible tetR-family transcriptional regulator, len: 204 aa; similar to many proposed regulators and hypothetical proteins e.g. TR:CAB46789 (EMBL:AL096811) putative tetR transcriptional regulator from Streptomyces coelicolor (251 aa) fasta scores; opt: 194, z-score: 252.1, E(): 1.1e-06, (35.3% identity in 102 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and Prosite match to PS01081 Bacterial regulatory proteins, tetR family signature. Also contains possible helix-turn-helix motif (+3.50 SD) 41-62 aa 4 2 12 5 [Reporter]SCO1606 (17L19)_PCR/3774 [Gene]SCO1606/6861 NC_003888 SCI35.28, possible membrane protein, len: 322 aa; similar to S. coelicolor TR:O69914 (EMBL:AL023861) putative secreted protein SC3C8.01 (302 aa), fasta scores; opt: 730 z-score: 709.0 E(): 3e-32, 48.0% identity in 275 aa overlap. Contains possible hydrophobic membrane spanning region 4 2 12 4 [Reporter]SCO1732 (18H19)_PCR/3773 [Gene]SCO1732/6859 NC_003888 SCI11.21, hypothetical protein, len: 44 aa; unknown function, possible CDS suggested by GC frameplot and the presence of a possible RBS 4 2 12 3 [Reporter]SCO1680 (19D19)_PCR/3772 [Gene]SCO1680/6857 NC_003888 SCI30A.01, partial CDS, probable gluconate permease, len: 359aa; similar to many eg. SW:GNTT_ECOLI high affinity gluconate transporter from Escherichia coli (437 aa) fasta scores; opt: 998, z-score: 1067.1, E(): 0, (42.1% identity in 359 aa overlap). Contains possible membrane spanning hydrophobic regions.,SCI52.22, probable gluconate permease (fragment), len: >144 aa; similar to N-terminal region of SW:GNUT_PSEAE (EMBL:AF026470) Pseudomonas aeruginosa gluconate permease GnuT or PA2322, 450 aa; fasta scores: opt: 370 Z-score: 442.1 bits: 89.4 E(): 4.8e-17; 44.262% identity in 122 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 12 2 [Reporter]SCO7704 (20L15)_PCR/3771 [Gene]SCO7704/6855 NC_003888 SCBAC12C8.05, possible TetR-family transcriptional regulator, len: 235 aa: similar to many from Streptomyces coelicolor e.g. TR:Q53901 (EMBL:M64683) ORF1-4 (ActII) (259 aa) fasta scores; opt: 310, Z-score: 362.1, 32.889% identity (35.071% ungapped) in 225 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and Pfam match to entry PF02909 tetR_C, Tetracyclin repressor, C-terminal all-alpha domain. 4 2 11 22 [Reporter]SCO1264 (1L11)_PCR/3769 [Gene]SCO1264/6853 NC_003888 2SCG18.11, probable hydrolase, len: 316 aa; similar to TR:CAB88827 (EMBL:AL353832) Streptomyces coelicolor putative hydrolase SCE6.23c, 294 aa; fasta scores: opt: 302 z-score: 345.4 E(): 9.3e-12; 31.5% identity in 276 aa overlap. Contains Pfam match to entry PF00561 abhydrolase, alpha/beta hydrolase fold 4 2 11 21 [Reporter]SCO1494 (2H11)_PCR/3768 [Gene]SCO1494/6851 NC_003888 SC9C5.18c, aroB, 3-dehydroquinate synthase, len: 363 aa; similar to SW:AROB_ECOLI (EMBL:X03867) Escherichia coli 3-dehydroquinate synthase (EC 4.6.1.3) AroB, 362 aa; fasta scores: opt: 699 z-score: 796.4 E(): 0; 37.5% identity in 352 aa overlap. Contains Pfam match to entry PF01761 DHQ_synthase, 3-dehydroquinate synthase 3 1 17 6 [Reporter]SCO5002 (17C4)_PCR/1365 [Gene]SCO5002/2499 NC_003888 SCK15.04c, conserved hypothetical protein, len: 216 aa; similar to TR:CAB77308 (EMBL:AL160312) Streptomyces coelicolor hypothetical 26.0 kD protein SCD35.24c, 241 aa; fasta scores: opt: 613 z-score: 732.4 E(): 0; 52.8% identity in 197 aa overlap 4 2 11 20 [Reporter]SCO1482 (3D11)_PCR/3767 [Gene]SCO1482/6849 NC_003888 SC9C5.06c, pyrD, dihydroorotate dehydrogenase, len: 368 aa; similar to SW:PYRD_ECOLI (EMBL:X02826) Escherichia coli dihydroorotate dehydrogenase (EC 1.3.3.1) PyrD, 336 aa; fasta scores: opt: 874 z-score: 976.6 E(): 0; 43.2% identity in 336 aa overlap. Contains Pfam match to entry PF01180 DHOdehase, Dihydroorotate dehydrogenase and match to Prosite entry PS00912 Dihydroorotate dehydrogenase signature 2 4 2 11 19 [Reporter]SCO2251 (4P7)_PCR/3766 [Gene]SCO2251/6847 NC_003888 SC1G2.13, possible integral membrane protein, len: 224 aa. Contains multiple possible membrane spanning hydrophobic domains. 4 2 11 18 [Reporter]SCO2205 (5L7)_PCR/3765 [Gene]SCO2205/6845 NC_003888 SC3H12.13c, unknown, len: 104 aa 4 2 11 17 [Reporter]SCO2055 (6H7)_PCR/3764 [Gene]SCO2055/6843 NC_003888 SC4G6.24, unknown, len: 525 aa; previously sequenced but annotated as two seperate hypothetical CDSs TR:Q53930 and TR:Q53931 (EMBL:X64420)) suggesting a sequence error which lead to a frameshift. Amino acid and codon usage plots support a single CDS. Also similar to TR:O88013 (EMBL:AL031107) hypothetical protein from Streptomyces coelicolor (527 aa) fasta scores; opt: 593, z-score: 699.2, E(): 1.3e-31, (36.0% identity in 545 aa overlap). Contains possible hydrophobic membrane spanning region at C-terminal domain 4 2 11 16 [Reporter]SCO7266 (7D7)_PCR/3763 [Gene]SCO7266/6841 NC_003888 SC5H1.26, probable 3-oxoacyl-[acyl-carrier protein] reductase, len: 245 aa; similar to many short-chain oxidoreductases e.g. SW:FABG_BACSU (EMBL:U59433), FabG, Bacillus subtilis 3-oxoacyl-[acyl-carrier protein] reductase (246 aa), fasta scores; opt: 521 z-score: 562.5 E(): 5.2e-24, 36.6% identity in 246 aa overlap and SW:DHG2_BACME (EMBL:D90044), GdhII, Bacillus megaterium glucose 1-dehydrogenase II (261 aa) (35.3% identity in 249 aa overlap). Similar to SCE134.03c (EMBL:AL049661) possible short chain dehydrogenase (255 aa) (35.1% identity in 242 aa overlap). Contains Pfam matches to entry PF00106 adh_short, short chain dehydrogenase, score 220.40, E-value 2.7e-62 and entry PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus, score 44.40, E-value 2.6e-09 3 1 16 22 [Reporter]SCO1326 (1O16)_PCR/1359 [Gene]SCO1326/2489 NC_003888 2SCG61.08, possible integral membrane lipid kinase, len: 291 aa; similar to many e.g. SW:P31054 (BACA_ECOLI) bacitracin resistance protein (putative undecaprenol kinase) (EC 2.7.1.66) from Escherichia coli (273 aa) fasta scores; opt: 283, z-score: 343.0, E(): 1.2e-11, 33.8% identity in 281 aa overlap and TR:O06239 (EMBL:Z95388) hypothetical protein from Mycobacterium tuberculosis (276 aa) fasta scores; opt: 439, z-score: 527.6, E(): 6.5e-22, 47.1% identity in 289 aa overlap. Contains possible membrane-spanning hydrophobic regions 4 2 11 14 [Reporter]SCO7807 (9L3)_PCR/3761 [Gene]SCO7807/6839 NC_003888 SC8E7.04c, unknown, len: 92 aa. Contains 2 TTA leucine codons, possible targets for bldA regulation 4 2 11 13 [Reporter]SCO5121 (10H3)_PCR/3760 [Gene]SCO5121/6837 NC_003888 SC9E12.06, probable peptide transport system ATP-binding protein, len: 356 aa; similar to SW:APPF_BACSU (EMBL:U20909) Bacillus subtilis oligopeptide transport ATP-binding protein AppF, 329 aa; fasta scores: opt: 1255 z-score: 1332.6 E(): 0; 57.3% identity in 335 aa overlap, to TR:P72410 (EMBL:U68036) Streptomyces coelicolor putative ABC transporter intracellular ATPase subunit BldKE (fragment), 284 aa; fasta scores: opt: 999 z-score: 1063.6 E(): 0; 58.1% identity in 260 aa overlap and to TR:O86575 (EMBL:AL031184) Streptomyces coelicolor oligopeptide ABC transporter ATP-binding protein SC2A11.14, 496 aa; fasta scores: opt: 1306 z-score: 1117.3 E(): 0; 55.4% identity in 345 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature 4 2 11 12 [Reporter]SCO2261 (11D3)_PCR/3759 [Gene]SCO2261/6835 NC_003888 SCC75A.07c, possible membrane protein, len: 85 aa. Contains a possible membrane spanning hydrophobic domains. 4 2 11 11 [Reporter]SCO4436 (11P23)_PCR/3758 [Gene]SCO4436/6833 NC_003888 SCD6.14, possible lyase, len: 136 aa; highly similar to TR:O86701 (EMBL:AL031515) Streptomyces coelicolor putative lyase SC5C7.04, 138 aa; fasta scores: opt: 532 z-score: 662.4 E(): 2e-29; 53.7% identity in 136 aa overlap 4 2 11 10 [Reporter]SCO2836 (12L23)_PCR/3757 [Gene]SCO2836/6831 NC_003888 SCE20.10, possible glycosyl transferase., len: 653 aa. Weakly similar to several other putative glycosyl transferases e.g. Aquifex aeolicus TR:O67406 (EMBL:AE000738) cellulose synthase catalytic subunit (759 aa), fasta scores opt: 326 z-score: 359.1 E(): 1.3e-12 22.0% identity in 590 aa overlap. Contains a Pfam match to entry PF00535 Glycos_transf_2, Glycosyl transferases and multiple possible membrane spanning hydrophobic domains 3 1 16 17 [Reporter]SCO6540 (6K12)_PCR/1354 [Gene]SCO6540/2479 NC_003888 SC5C7.25, unknown, len: 101 aa; similar to several genes predicted to be pterin-4a-carbinolamine dehydratases e.g. Aquifex aeolicus TR:O66462 (EMBL:AE000671) phhB (99 aa), fasta scores; opt: 165 z-score: 280.7 E(): 2.2e-08, 30.3% identity in 89 aa overlap 4 2 11 9 [Reporter]SCO3643 (13H23)_PCR/3756 [Gene]SCO3643/6829 NC_003888 SCH10.21c, hypothetical protein, len: 329 aa; unknown function, weakly similar to SW:YT11_MYCTU (EMBL:Z79700) Mycobacterium tuberculosis hypothetical protein (266 aa), fasta scores; opt: 183 z-score: 199.4 E(): 0.00086, 30.7% identity in 225 aa overlap 4 2 11 8 [Reporter]SCO0902 (14D23)_PCR/3755 [Gene]SCO0902/6827 NC_003888 SCM1.35c, unknown, len: 142 aa. Similar to the C-terminus of several catechol 2,3-dioxygenases e.g. Sphingomonas sp. A8AN3 TR:Q9ZAY3(EMBL:U73127) catechol 2,3-dioxygenase (307 aa), fasta scores opt:128 z-score: 160.4 E():0.14 31.2% identity in 141 aa overlap. 4 2 11 7 [Reporter]SCO1038 (15P19)_PCR/3754 [Gene]SCO1038/6825 NC_003888 SCG20A.18, hypothetical protein, len: 115 aa; similar to TR:O06633 (EMBL:Z95618) Mycobacterium tuberculosis hypothetical 12.5 kD protein aa; fasta scores: opt: 138 z-score: 194.2 E(): 0.0024; 34.0% identity in 97 aa overlap 4 2 11 6 [Reporter]SCO5170 (16L19)_PCR/3753 [Gene]SCO5170/6823 NC_003888 SCP8.33c, probable tetR-family transcriptional regulator, len: 213 aa; similar to TR:O53661 (EMBL:AL021929) Mycobacterium tuberculosis putative regulatory protein MTV034.04, 204 aa; fasta scores: opt: 309 z-score: 383.4 E(): 6.9e-14; 31.3% identity in 201 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and possible helix-turn-helix motif at residues 42..63 (+3.37 SD) 4 2 11 5 [Reporter]SCO3153 (17H19)_PCR/3752 [Gene]SCO3153/6821 NC_003888 SCE87.04c, conserved hypothetical protein, len: 286 aa. Highly similar to many including: Mycobacterium tuberculosis SW:YA03_MYCTU (EMBL:Z94752) hypothetical 29.7 KD protein RV1003 (285 aa), fasta scores opt: 877 z-score: 992.7 E():0 53.6% identity in 278 aa overlap and Bacillus subtilis SW:YABC_BACSU (EMBL:D26185) hypothetical 33.0 KD protein (292 aa), fasta scores opt: 640 z-score: 726.6 E():0 41.3% identity in 276 aa overlap. Contains a Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases. 3 1 16 12 [Reporter]SCO2549 (11G8)_PCR/1349 [Gene]SCO2549/2469 NC_003888 SCC77.16c, possible protease, len: 1067 aa. Similar to Thermoplasma acidophilum TR:P96086(EMBL:U72850) tricorn protease (1071 aa), fasta scores opt:opt: 937 z-score: 985.8 E():0 29.9% identity in 1091 aa overlap and Streptomyces coelicolor TR:CAB59664(EMBL:AL132674) putative protease SCE87.19 (1171 aa), fasta scores opt: 2252 z-score: 2374.0 E():0 48.1% identity in 1159 aa overlap. 4 2 11 4 [Reporter]SCO0182 (18D19)_PCR/3751 [Gene]SCO0182/6819 NC_003888 SCJ1.31c, doubtful CDS, len: 74 aa. Contains a TTA/leucine codon, possible target for bldA regulation. 4 2 11 3 [Reporter]SCO1309 (19P15)_PCR/3750 [Gene]SCO1309/6817 NC_003888 SCBAC36F5.20c, possible regulatory protein, len: 115 aa; similar to TR:O53838 (EMBL:AL022004) Mycobacterium tuberculosis putative transcriptional regulator RV0827c or MTV043.19c, 130 aa; fasta scores: opt: 243 Z-score: 311.9 bits: 63.2 E(): 9.2e-10; 39.252% identity in 107 aa overlap 4 2 11 2 [Reporter]SCO2821 (20H15)_PCR/3749 [Gene]SCO2821/6815 NC_003888 SCBAC17F8.12c, possible secreted pectate lyase, len: 521 aa: similar to many e.g. TR:Q9KGY6 (EMBL:AF278705) pectate lyase B from Alteromonas haloplanktis (658 aa) fasta scores; opt: 1744, Z-score: 1590.1, 63.301% identity (65.859% ungapped) in 515 aa overlap and SW:P04959 (PELB_ERWCH) pectate lyase B from Erwinia chrysanthemi (375 aa) fasta scores; opt: 413, Z-score: 386.2, 29.167% identity (32.464% ungapped) in 384 aa overlap. Contains Pfam match to entry PF00544 pec_lyase, Pectate lyase and a probable N-terminal signal sequence. 4 2 10 22 [Reporter]SCO2802 (1H11)_PCR/3747 [Gene]SCO2802/6813 NC_003888 2SCC13.10, possible secreted protein, len: 193 aa. Contains possible N-terminal region signal peptide sequence 4 2 10 21 [Reporter]SCO1579 (2D11)_PCR/3746 [Gene]SCO1579/6811 NC_003888 SCL24.15c, argJ, glutamate N-acetyltransferase (fragment), len: >228 aa; highly similar to TR:P94988 (EMBL:Z85982) Mycobacterium tuberculosis ArgJ, 404 aa; fasta scores: opt: 782 z-score: 919.7 E(): 0; 57.1% identity in 226 aa overlap and to SW:ARGJ_CORGL (EMBL:X86157) Corynebacterium glutamicum glutamate N-acetyltransferase (EC 2.3.1.35) ArgJ, 388 aa; fasta scores: opt: 730 z-score: 859.3 E(): 0; 50.4% identity in 230 aa overlap. Contains Pfam match to entry PF01960 ArgJ, ArgJ family,SCI35.01c, argJ, probable glutamate N-acetyltransferase, partial CDS, len: >184 aa; similar to many e.g. ARGJ_CORGL glutamate N-acetyltransferase (ornithine acetyltransferase) (EC 2.3.1.35) (388 aa), fasta scores; opt: 614 z-score: 909.0 E(): 0, 55.4% identity in 184 aa overlap. Referred to as 'argB' on genetic map 3 1 16 7 [Reporter]SCO2939 (16C4)_PCR/1344 [Gene]SCO2939/2459 NC_003888 SCE56.03c, unknown, len: 260 aa 4 2 10 20 [Reporter]SCO4706 (3P7)_PCR/3745 [Gene]SCO4706/6809 NC_003888 SCD31.31, rpsS, 30S ribosomal protein S19, len: 93 aa; highly similar to SW:RS19_BACST (EMBL:X54994) Bacillus stearothermophilus 30S ribosomal protein S19 RpsS, 91 aa; fasta scores: opt: 448 z-score: 642.9 E(): 2.2e-28; 67.8% identity in 90 aa overlap. Contains Pfam match to entry PF00203 Ribosomal_S19, Ribosomal protein S19 and match to Prosite entry PS00323 Ribosomal protein S19 signature 4 2 10 19 [Reporter]SCO2247 (4L7)_PCR/3744 [Gene]SCO2247/6807 NC_003888 SC1G2.09c, unknown, len: 61 aa. 4 2 10 18 [Reporter]SCO5735 (5H7)_PCR/3743 [Gene]SCO5735/6805 NC_003888 SC3C3.21, probable integral membrane protein, len: 491 aa; similar to hypothetical membrane protein from M. tuberculosis TR:O06265 (EMBL:Z95389) MTCY77.20 Rv3448 (467 aa), fasta scores; opt: 192 z-score: 322.0 E(): 1.1e-10, 26.9% identity in 495 aa overlap 4 2 10 17 [Reporter]SCO2051 (6D7)_PCR/3742 [Gene]SCO2051/6803 NC_003888 SC4G6.20c, hisH, amidotransferase, len: 222aa; previously sequenced therefoer identical to SW:HIS5_STRCO. Contains Pfam match to entry PF00117 GATase, Glutamine amidotransferase class-I and Prosite match to PS00442 Glutamine amidotransferases class-I active site. 4 2 10 16 [Reporter]SCO3617 (7P3)_PCR/3741 [Gene]SCO3617/6801 NC_003888 SC66T3.28c, hypothetical protein, len: 219 aa; unknown function, probable CDS suggested by positional base preference, GC frame analysis and amino acid composition 3 1 16 2 [Reporter]SCO5105 (20K20)_PCR/1339 [Gene]SCO5105/2449 NC_003888 SCBAC31E11.01, possible membrane protein, len: 410 aa. Contains possible hydrophobic membrane spanning region 4 3 10 18 [Reporter]SCO5727 (5H6)_PCR/5759 [Gene]SCO5727/10493 NC_003888 SC3C3.13, unknown, len: 200 aa 4 3 10 17 [Reporter]SCO2043 (6D6)_PCR/5758 [Gene]SCO2043/10491 NC_003888 SC4G6.12c, trpE3, probable anthranilate synthase component I, len: 502aa; similar to many eg. SW:TRPE_ARTGO anthranilate synthase component I from Arthrobacter globiformis (531 aa) fasta scores; opt: 1921, z-score: 2150.9, E(): 0, (60.6% identity in 500 aa overlap). Contains Pfam match to entry PF00425 chorismate_bind, chorismate binding enzyme. 3 1 3 20 [Reporter]SCO7336 (3C4)_PCR/1071 [Gene]SCO7336/1969 NC_003888 SC4G10.15c, pepA, unknown, len: 90 aa. Previously sequenced region: Streptomyces coelicolor TR:O54206(EMBL:AJ001206) hypothetical protein, PepA (90 aa). 3 1 3 19 [Reporter]SCO6152 (3O24)_PCR/1070 [Gene]SCO6152/1967 NC_003888 SC1A9.16c, small hydrophobic protein (putative membrane protein), len: 160 aa. Contains possible hydrophobic membrane spanning regions 3 1 3 18 [Reporter]SCO7086 (4K24)_PCR/1069 [Gene]SCO7086/1965 NC_003888 SC3A4.12c, possible MerR-family transcriptional regulator, len: 115 aa. Similar to many regulators including: Thiobacillus ferrooxidans SW:MERR_THIFE (EMBL:M57717) mercuric resistance operon regulatory protein (fragment) (122 aa), fasta scores opt: 166 z-score: 229.9 E(): 2.4e-05 33.0% identity in 106 aa overlap. Conatins a Prosite hit to PS00552 Bacterial regulatory proteins, merR family signature and a Pfam match to entry PF00376 merR, Bacterial regulatory proteins, merR family with a putative helix-turn-helix motif situated between residues 4..25 (+4.59 SD). 4 3 10 16 [Reporter]SCO3609 (7P2)_PCR/5757 [Gene]SCO3609/10489 NC_003888 SC66T3.20, possible membrane protein, len: 623 aa; unknown function, C-terminus shares weak similarity with other hypothetical membrane proteins e.g. TR:P74405 (EMBL:D90914) Synechocystis sp. hypothetical protein (568 aa), fasta scores; opt: 250 z-score: 272.0 E(): 8e-08, 24.3% identity in 576 aa overlap. Contains hydrophobic, possible membrane-spanning regions 3 1 3 17 [Reporter]SCO6562 (5G24)_PCR/1068 [Gene]SCO6562/1963 NC_003888 SC4B5.12c, probable oxidoreductase, len: 323; similar to several hypothetical /putative= probable 2-dehydropantoate 2-reductase from Archaeoglobus fulgidus (294 aa), fasta scores; opt: 245, z-score: 404.0, E(): 3.3e-15, (29.1% identity in 313 aa overlap). 4 2 22 10 [Reporter]SCO3009 (13H11)_PCR/3999 [Gene]SCO3009/7269 NC_003888 SCE33.11c, hypothetical protein, len: 254 aa; similar to TR:O05886 (EMBL:Z95121) Mycobacterium tuberculosis hypothetical 24.5 kD protein MTCY20B11.16c, 214 aa; fasta scores: opt: 487 z-score: 551.0 E(): 3.1e-23; 47.6% identity in 210 aa overlap 4 3 10 15 [Reporter]SCO5569 (8L2)_PCR/5756 [Gene]SCO5569/10487 NC_003888 SC7A1.13, unknown, len: 379 aa; similar to TR:O69467 (EMBL:AL023635) hypothetical protein from Mycobacterium tuberculosis (245 aa) fasta scores; opt: 254, z-score: 388.8, E(): 2.3e-14, (29.5% identity in 234 aa overlap). Contains probable coiled-coil from 82 to 124 (43 residues) Max score: 1.615 (probability 1.00). 4 2 22 9 [Reporter]SCO0686 (14D11)_PCR/3998 [Gene]SCO0686/7267 NC_003888 SCF15.07, possible membrane protein, 407 aa. Similar in parts to several hypothetical proteins e.g. Bacillus subtilis TR:O34437(EMBL:Z99108) hypothetical YfkH protein (275 aa), fasta scores: opt: 542 z-score: 589.5 E(): 1.8e-25 32.0% identity in 284 aa overlap. Contains multiple possible membrane spanning hydrophobic domains 4 3 10 14 [Reporter]SCO5674 (9H2)_PCR/5755 [Gene]SCO5674/10485 NC_003888 SC8B7.04, probable integral membrane protein, len: 247 aa. Contains possible hydrophobic membrane spanning regions 4 2 22 8 [Reporter]SCO3556 (15P7)_PCR/3997 [Gene]SCO3556/7265 NC_003888 SCH5.19c, possible secretory protein, len: 523 aa; similar to many of undefined function eg. TR:O69627 (EMBL:AL022121) hypothetical protein from Mycobacterium tuberculosis (352 aa) fasta scores; opt: 1287, z-score: 1191.3, E(): 0, (59.8% identity in 351 aa overlap) and SW:TRBB_AGRT6 conjugal transfer protein from Agrobacterium tumefaciens (323 aa) fasta scores; opt: 469, z-score: 441.4, E(): 2.8e-17, (31.4% identity in 287 aa overlap). Contains Pfam match to entry PF00437 GSPII_E, Bacterial type II secretion system protein, score 41.30, E-value 1.6e-11. Also contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00041 Bacterial regulatory proteins, araC family signature. 4 3 10 13 [Reporter]SCO6485 (10D2)_PCR/5754 [Gene]SCO6485/10483 NC_003888 SC9C7.21, unknown, len: 240 aa 4 2 22 7 [Reporter]SCO1233 (16L7)_PCR/3996 [Gene]SCO1233/7263 NC_003888 2SCG1.08c, ureF, urease accessory protein, len: 224 aa; similar to SW:UREF_MYCTU (EMBL:U33011) Mycobacterium tuberculosis urease accessory protein UreF, 211 aa; fasta scores: opt: 401 z-score: 448.6 E(): 1.6e-17; 38.8% identity in 224 aa overlap. Contains Pfam match to entry PF01730 UreF, UreF and match to Prosite entry PS00099 Thiolases active site 4 3 10 12 [Reporter]SCO2767 (10P22)_PCR/5753 [Gene]SCO2767/10481 NC_003888 SCC57A.38c, possible ATP/GTP-binding protein, len: 743 aa. Weakly similar to another Streptomyces coelicolor TR:O86768(EMBL:AL031035) putative ATP/GTP-binding protein (886 aa), fasta scores opt: 328 z-score: 356.5 E(): 1.8e-12 28.9% identity in 699 aa overlap. Contains a Prosite hit to PS00017 ATP/GTP-binding site motif A (P-loop). 4 2 22 6 [Reporter]SCO1515 (17H7)_PCR/3995 [Gene]SCO1515/7261 NC_003888 SCL2.05c, secF, protein-export membrane protein, len: 373 aa; identical to previously sequenced SW:SECF_STRCO (EMBL:X85969) Streptomyces coelicolor protein-export membrane protein SecF, 373 aa and similar to SW:SECF_ECOLI (EMBL:X56175) Escherichia coli protein-export membrane protein SecF, 323 aa; fasta scores: opt: 488 z-score: 529.1 E(): 4.4e-22; 31.2% identity in 314 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 22 4 [Reporter]SCO6106 (19P3)_PCR/3993 [Gene]SCO6106/7259 NC_003888 SCBAC1A6.30c, possible acyl-CoA dehydrogenase, len: 557 aa; similar to SW:AIDB_ECOLI (EMBL:L20915) Escherichia coli AidB protein, 541 aa; fasta scores: opt: 916 z-score: 1055.7 E(): 0; 45.5% identity in 539 aa overlap and to TR:CAC13064 (EMBL:AL445503) Streptomyces coelicolor putative oxidoreductase 2SCG38.05, 567 aa; fasta scores: opt: 1480 z-score: 1414.3 E(): 0; 61.8% identity in 555 aa overlap. Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase and match to Prosite entry PS00072 Acyl-CoA dehydrogenases signature 1 and PS00073 Acyl-CoA dehydrogenases signature 2 4 3 10 10 [Reporter]SCO3389 (12H22)_PCR/5751 [Gene]SCO3389/10477 NC_003888 SCE126.07c, probable two component system response regulator, len: 224aa; similar to many, particularly others from Streptomyces coelicolor eg. TR:O86632 (EMBL:AL031155) putative two component regulator (222 aa) fasta scores; opt: 784, z-score: 897.1, E(): 0, (57.7% identity in 215 aa overlap). Also similar to SW:DEGU_BACSU transcriptional regulator protein DegU from Bacillus subtilis (229 aa) fasta scores; opt: 466, z-score: 538.0, E(): 1.2e-22, (37.2% identity in 223 aa overlap). Contains Pfam matches to entry PF00072 response_reg, Response regulator receiver domain and to entry PF00196 GerE, Bacterial regulatory proteins, luxR family. Also contains Prosite match to PS00622 Bacterial regulatory proteins, luxR family signature. 4 2 22 3 [Reporter]SCO4901 (20L3)_PCR/3992 [Gene]SCO4901/7257 NC_003888 2SCK8.27, probable adenosine deaminase, len: 396 aa; similar to SW:ADD_ECOLI (EMBL:M59033) Escherichia coli adenosine deaminase (EC 3.5.4.4) Add, 333 aa; fasta scores: opt: 421 z-score: 505.2 E(): 1.4e-20; 32.2% identity in 338 aa overlap and to TR:Q9X7T2 (EMBL:AL049863) Streptomyces coelicolor putative adenosine deaminase SC5H1.24c, 359 aa; fasta scores: opt: 1373 z-score: 1637.4 E(): 0; 60.7% identity in 351 aa overlap. Contains Pfam match to entry PF00962 A_deaminase, Adenosine/AMP deaminase 4 3 10 9 [Reporter]SCO3341 (13D22)_PCR/5750 [Gene]SCO3341/10475 NC_003888 SCE7.08c, possible integral membrane protein, len: 167aa; Contains possible membrane spanning hydrophobic regions. 4 2 21 22 [Reporter]SCO1091 (1D23)_PCR/3989 [Gene]SCO1091/7255 NC_003888 2SCG4.07c, possible acetyltransferase, len: 179 aa; similar to TR:Q9ZBJ7 (EMBL:AL035161) Streptomyces coelicolor hypothetical 19.8 kD protein SC9C7.13c, 179 aa; fasta scores: opt: 131 z-score: 171.3 E(): 0.047; 29.3% identity in 157 aa overlap. Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family 4 3 10 8 [Reporter]SCO4873 (14P18)_PCR/5749 [Gene]SCO4873/10473 NC_003888 SCK20.14c, unknown, len: 312 aa 4 2 21 21 [Reporter]SCO2609 (2P19)_PCR/3988 [Gene]SCO2609/7253 NC_003888 SCC88.20c, mreD, rod shape-determining protein, len: 223 aa; identical to previously sequenced TR:O33621 (EMBL:Y14206) Streptomyces coelicolor MreD protein, 223 aa and similar to TR:O83512 (EMBL:AE001226) Treponema pallidum rod shape-determining protein (MreD), 168 aa; fasta scores: opt: 135 z-score: 162.4 E(): 0.13; 24.8% identity in 161 aa overlap. Contains possible N-terminal region signal peptide sequence 4 3 10 7 [Reporter]SCO3555 (15L18)_PCR/5748 [Gene]SCO3555/10471 NC_003888 SCH5.18c, possible integral membrane protein, len: 321 aa; similar to TR:O69626 (EMBL:AL022121) hypothetical protein (266 aa) fasta scores; opt: 335, z-score: 348.0, E(): 4.5e-12, (38.1% identity in 247 aa overlap). Contains probable coiled-coil from 227 to 264 (38 residues), max score: 1.545, (probability 0.98). 4 2 21 20 [Reporter]SCO6430 (3L19)_PCR/3987 [Gene]SCO6430/7251 NC_003888 SC1A6.19, possible integral membrane protein, len: 630 aa; N-terminus (start to aa ~400) is hydrophobic, C-terminus is proline-rich 4 2 21 19 [Reporter]SCO5618 (4H19)_PCR/3986 [Gene]SCO5618/7249 NC_003888 SC2E1.35, unknown prophage gene, len: 147 aa 4 3 10 5 [Reporter]SCO0198 (17D18)_PCR/5746 [Gene]SCO0198/10467 NC_003888 SCJ12.10c, unknown, len: 288 aa. Similar to several hypothetical proteins including: Mycobacterium tuberculosis SW:YW12_MYCTU (EMBL; Z74025) hypothetical 30.9 KD protein MTCY39.12 (295 aa), fasta scores opt: 363 z-score: 441.0 E(): 3.3e-17 32.3% identity in 288 aa overlap and Mycobacterium tuberculosis TR:O06189 (EMBL; Z95387) hypothetical 31.7 KD protein MTCY1A10.09C (297 aa), fasta scores opt: 336 z-score: 408.6 E(): 2.1e-15 29.3% identity in 294 aa overlap. Also highly similar to S. coelicolor hypothetical proteins (AL109962) SCJ1.29c (283 aa), fasta scores opt: 1161 z-score: 1097.2 E(): 0 63.5% identity in 288 aa overlap and SCJ1.16c (294 aa), fasta scores opt:892 z-score: 844.7 E():0 50.3% identity in 296 aa overlap 4 2 21 18 [Reporter]SCO7101 (5D19)_PCR/3985 [Gene]SCO7101/7247 NC_003888 SC4B10.02, probable dehydrogenase, len: 451 aa; similar to SW:DHNA_ECOLI (EMBL:V00306) Escherichia coli NADH dehydrogenase (EC 1.6.99.3) Ndh, 433 aa; fasta scores: opt: 472 z-score: 552.4 E(): 2.7e-23; 31.6% identity in 377 aa overlap 4 3 10 4 [Reporter]SCO6091 (18P14)_PCR/5745 [Gene]SCO6091/10465 NC_003888 SCBAC1A6.15c, probable integral membrane protein, len: 756 aa; similar to SW:MMLB_MYCTU (EMBL:AL021928) Mycobacterium tuberculosis putative membrane protein MmpL11 or MTV033.10c, 966 aa; fasta scores: opt: 1132 z-score: 1196.3 E(): 0; 32.6% identity in 723 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 21 17 [Reporter]SCO2184 (6P15)_PCR/3984 [Gene]SCO2184/7245 NC_003888 SC5F7.17, possible transcriptional regulator, len: 327aa; similar to TR:O34920 (EMBL:AF027868) putative transcriptional regulator from Bacillus subtilis (313 aa) fasta scores; opt: 319, z-score: 380.6, E(): 7.3e-14, (27.3% identity in 330 aa overlap). Contains helix-turn-helix motif (+4.15 SD) 20-41aa. 4 3 10 3 [Reporter]SCO6101 (19L14)_PCR/5744 [Gene]SCO6101/10463 NC_003888 SCBAC1A6.25c, unknown, len: 59 aa 4 2 21 16 [Reporter]SCO1407 (7L15)_PCR/3983 [Gene]SCO1407/7243 NC_003888 SC6D7.32, unknown, len: > 1425 aa. Weakly similar to several including: Streptomyces coelicolor TR:Q9Z5A4(EMBL:AL035478) putative secreted protein SC2G5.19 (1156 aa), fasta scores opt: 327 z-score: 314.0 E(): 4.1e-10 28.0% identity in 938 aa overlap and Rhodobacter capsulatus TR:O68032(EMBL:AF010496) potential exonuclease (1238 aa), fasta scores opt: 439 z-score: 421.6 E(): 4.1e-16 28.8% identity in 1154 aa overlap. The predicted protein product of this CDS is alanine rich and contains multiple probable coiled-coils between residues: 369 to 401 (33 residues), 406 to 433 (28 residues), 483 to 519 (37 residues), 563 to 604 (42 residues),850 to 921 (72 residues),926 to 982 (57 residues), 1025 to 1054 (30 residues),1072 to 1106 (35 residues), 1124 to 1167 (44 residues), 1199 to 1232 (34 residues) and 1278 to 1309 (32 residues).,SC1A8A.26c, partial CDS, unknown, len: >162aa; forms the C-terminal region of SC6D7.32 (TR:CAB61684 EMBL:AL133213) which has weak similarity to proteins of unknown function. 4 3 10 2 [Reporter]SCO2820 (20D14)_PCR/5743 [Gene]SCO2820/10461 NC_003888 SCBAC17F8.11c, hypothetical protein, len: 115 aa: no significant database matches. 4 2 21 15 [Reporter]SCO5234 (8H15)_PCR/3982 [Gene]SCO5234/7241 NC_003888 SC7E4.31, possible sugar transporter integral membrane protein, len: 276 aa; similar to TR:Q9RK77 (EMBL:AL132662;) Streptomyces coelicolor putative sugar transporter integral membrane protein SCF11.13, 285 aa; fasta scores: opt: 1027 z-score: 1227.9 E(): 0; 54.3% identity in 278 aa overlap and to TR:Q9R9Q5 (EMBL:AF175299) Rhizobium meliloti putative inner membrane permease ThuG, 276 aa; fasta scores: opt: 554 z-score: 666.6 E(): 1.2e-29; 33.8% identity in 266 aa overlap. Contains Pfam match to entry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component and match to Prosite entry PS00402 Binding-protein-dependent transport systems inner membrane comp sign. Contains also possible hydrophobic membrane spanning regions 4 2 21 14 [Reporter]SCO7534 (9D15)_PCR/3981 [Gene]SCO7534/7239 NC_003888 SC8G12.10, possible two component system histidine kinase, len: 504 aa. Highly similar to several e.g. Streptomyces coelicolor SW:AFQ2_STRCO(EMBL:D10654) sensor protein AfsQ2 (EC 2.7.3.-) (535 aa), fasta scores opt: 1060 z-score: 1143.7 E(): 0 41.3% identity in 506 aa overlap. Contains Pfam matches to entries PF00672 DUF5, Domain found in bacterial signal proteins and PF00512 signal, Histidine kinase domain, as well as a possible N-terminal hydrophobic membrane spanning region 4 3 9 20 [Reporter]SCO5624 (3L6)_PCR/5739 [Gene]SCO5624/10457 NC_003888 SC2E1.41, rpsB, 30S ribosomal protein S2, len: 310 aa; almost identical to RS2_STRCO 30S ribosomal protein S2 (242 aa), E(): 0, 96.7% identity in 242 aa overlap and also highly similar to many others e.g. RS2_ECOLI 30S ribosomal protein S2 (240 aa), fasta scores; opt: 744 z-score: 950.7 E(): 0, 50.4% identity in 224 aa overlap. Contains PS00962 Ribosomal protein S2 signature 1 and PS00211 ABC transporters family signature (probably a false positive). Also contains Pfam matches to entry S2 PF00318, Ribosomal protein S2, score 304.89 and to entry 60s_ribosomal PF00428, 60s Acidic ribosomal protein, score 24.36. Note that this sequence is longer than RS2_STRCO due to a sequence difference around 235 aa. This sequence has a C-terminal extension not present in other rpsB. The M. tuberculosis protein also has a C-terminal extension but with a differentsequence. Our sequence has been checked, and is also confirmed by sequence from the overlapping cosmid 6A9. 4 2 21 13 [Reporter]SCO2583 (10P11)_PCR/3980 [Gene]SCO2583/7237 NC_003888 SCC123.21c, possible membrane protein, len: 356 aa. similar to TR:Q9L0J6 (EMBL:AL161691) SCD40A.15 putative membrane protein from Streptomyces coelicolor (379 aa) fasta scores; opt: 350, Z-score: 385.8, E(): 7.4e-14, 30.056% identity (33.025% ungapped) in 356 aa overlap. Contains a possible membrane spanning hydrophobic domain. 4 3 9 19 [Reporter]SCO6046 (4H6)_PCR/5738 [Gene]SCO6046/10455 NC_003888 SC1B5.06c, unknown, len: 249 aa 4 2 21 12 [Reporter]SCO4340 (11L11)_PCR/3979 [Gene]SCO4340/7235 NC_003888 SCD12A.23, possible integrase, len: 549 aa; similar to TR:CAB75384 (EMBL:AL139298) Streptomyces coelicolor putative integrase SCC88.14, 518 aa; fasta scores: opt: 232 z-score: 262.7 E(): 3.6e-07; 26.5% identity in 548 aa overlap 4 3 9 18 [Reporter]SCO5722 (5D6)_PCR/5737 [Gene]SCO5722/10453 NC_003888 SC3C3.08, serine protease (putative secreted protein), len: 413 aa; almost identical to TR:Q53863 (EMBL:U33176) serine protease (390 aa), 98.4% identity in 387 aa overlap and similar to many e.g. ELYA_BACSP alkaline elastase yab precursor (378 aa), fasta scores; opt: 466 z-score: 512.6 E(): 2.7e-21, 36.3% identity in 281 aa overlap. Also similar to downstream putative serine protease, SC3C3.17c (E(): 5.2e-07, 35.0% identity in 383 aa overlap). Contains PS00136, PS00137 and PS00138 Serine proteases, subtilase family, aspartic acid, histidine and serine active sites and Pfam match to entry PF00082 subtilase, Subtilase family of serine proteases, score 214.90, E-value 1.2e-60. Contains possible N-terminal region signal peptide sequence and possible hydrophobic membrane spanning region at C-terminal region 4 2 21 11 [Reporter]SCO4180 (12H11)_PCR/3978 [Gene]SCO4180/7233 NC_003888 SCD66.17, probable iron uptake regulatory protein, len: 145 aa; similar to SW:FUR_CAMJE (EMBL:X78965) Campylobacter jejuni ferric uptake regulation protein Fur, 157 aa; fasta scores: opt: 274 z-score: 366.6 E(): 6e-13; 31.9% identity in 135 aa overlap. Contains Pfam match to entry PF01475 FUR, Ferric uptake regulator family 4 3 9 17 [Reporter]SCO6658 (6P2)_PCR/5736 [Gene]SCO6658/10451 NC_003888 SC5A7.08c, probable 6-phosphogluconate dehydrogenase, len: 293 aa; similar to many eukaryotic e.g. 6PGD_BACSU 6-phosphogluconate dehydrogenase, decarboxylating (468 aa), fasta sores; opt: 445 z-score: 616.2 E(): 4.4e-27, 36.6% identity in 287 aa overlap.also highly similar to S. coelicolor 6-phosphogluconate dehydrogenase (EMBL:L27063) TR:Q53917 (291 aa), fasta sores; opt: 1654 z-score: 2054.7 E(): 0, 83.4% identity in 289 aa overlap. Contains PS00065 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature and Pfam match to entry PF00393 6PGD, 6-phosphogluconate dehydrogenases, score 169.20, E-value 6.9e-47 4 2 21 10 [Reporter]SCO3004 (13D11)_PCR/3977 [Gene]SCO3004/7231 NC_003888 SCE33.06, unknown, len: 89 aa. High content in arginine amino acid residues and also high % G+C (77.4%) 4 2 21 9 [Reporter]SCO0937 (14P7)_PCR/3976 [Gene]SCO0937/7229 NC_003888 SCM10.25, hypothetical protein, len: 153 aa; similar to various hypothetical proteins, e.g. TR:BAA86264 (EMBL:AB023785) Streptomyces griseus ORF2, 173 aa; fasta scores: opt: 218 z-score: 266.9 E(): 1.8e-07; 35.5% identity in 138 aa overlap 4 3 9 15 [Reporter]SCO2155 (8H2)_PCR/5734 [Gene]SCO2155/10447 NC_003888 SC6G10.28c, cox1, cytochrome c oxidase subunit I, len: 578aa; similar to many eg. SW:COX1_BACFI cytochrome c oxidase polypeptide I from Bacillus firmus (624 aa) fasta scores; opt: 1835, z-score: 2056.2, E(): 0, (49.5% identity in 527 aa overlap). Contains Pfam match to entry PF00115 COX1, Cytochrome C and Quinol oxidase polypeptide I and Prosite matches to PS00152 ATP synthase alpha and beta subunits signature and PS00077 Heme-copper oxidase catalytic subunit, copper B binding region signature. Also contains several probable membrane spanning hydrophobic regions. 4 2 21 8 [Reporter]SCO5036 (15L7)_PCR/3975 [Gene]SCO5036/7227 NC_003888 SCK7.09c, hypothetical protein, len: 174 aa; similar to TR:O50432 (EMBL:AL010186) Mycobacterium tuberculosis hypothetical 21.1 kDa protein MTV005.12c, 189 aa; fasta scores: opt: 183 z-score: 238.8 E(): 7.8e-06; 30.1% identity in 186 aa overlap 4 3 9 13 [Reporter]SCO1502 (9P22)_PCR/5732 [Gene]SCO1502/10445 NC_003888 SC9C5.26c, possible secreted protein, len: 118 aa. Contains possible N-terminal signal peptide sequence 4 2 21 7 [Reporter]SCO0212 (16H7)_PCR/3974 [Gene]SCO0212/7225 NC_003888 SCJ12.24c, unknown, len: 157 aa. 4 3 9 11 [Reporter]SCO4093 (11H22)_PCR/5730 [Gene]SCO4093/10443 NC_003888 SCD25.29c, possible integral membrane protein, len: 539 aa. Contains possible membrane spanning hydrophobic domains. 4 2 21 6 [Reporter]SCO0249 (17D7)_PCR/3973 [Gene]SCO0249/7223 NC_003888 SCJ9A.28c, possible monooxygenase, len: 504 aa; similar to many e.g. TR:Q9ZGM8 (EMBL:AF077869) oxygenase involved in the last step of mithramycin biosynthesis in Streptomyces argillaceus (533 aa) fasta scores; opt: 1215, z-score: 1311.5, E(): 0, (45.0% identity in 513 aa overlap). Contains Pfam matches to entry PF01360 Monooxygenase, Monooxygenase and Pfam match to entry PF01494 FAD_binding_3, and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible uncleavable N-terminal signal sequence. 4 3 9 10 [Reporter]SCO3385 (12D22)_PCR/5729 [Gene]SCO3385/10441 NC_003888 SCE126.03, possible lyase, len: 403aa; weakly similar to many eg. SW:LTAA_AERJA L-allo-threonine aldolase from Aeromonas jandaei DK-39 (338 aa) fasta scores; opt: 257, z-score: 293.4, E(): 5e-09, (31.4% identity in 255 aa overlap). 4 2 21 5 [Reporter]SCO3354 (18P3)_PCR/3972 [Gene]SCO3354/7221 NC_003888 SCE94.05, unknown, proline-rich, len: aa; 4 2 21 4 [Reporter]SCO6275 (19L3)_PCR/3971 [Gene]SCO6275/7219 NC_003888 SC1G7.01c, probable type I polyketide synthase (fragment), len: >3488 aa; similar to the N-terminal region of TR:Q9L4W3 (EMBL:AF263912) Streptomyces noursei nystatin polyketide synthase NysC, 11096 aa; fasta scores: opt: 7454 Z-score: 6765.5 bits: 1268.6 E(): 0; 45.788% identity in 4285 aa overlap, to TR:O33956 (EMBL:U78289) Streptomyces fradiae tylactone synthase modules 4 & 5 TylG, 3729 aa; fasta scores: opt: 3409 Z-score: 3092.3 bits: 587.4 E(): 1.2e-164; 44.860% identity in 2568 aa overlap and to TR:CAC22145 (EMBL:AL512902) Streptomyces coelicolor SC2C4.04c, 3577 aa; fasta scores: opt: 4872 Z-score: 3412.6 bits: 646.6 E(): 2.3e-184; 58.281% identity in 2560 aa overlap. Contains Pfam matches to entries 3x PF00109 ketoacyl-synt, Beta-ketoacyl synthase, 3x PF02801 ketoacyl-synt_C, Beta-ketoacyl synthase, 3x PF00698 Acyl_transf, Acyl transferase domain, PF00550 pp-binding, Phosphopantetheine attachment site, PF00550 pp-binding, Phosphopantetheine attachment site and matches to Prosite entries PS00012 Phosphopantetheine attachment site and PS00606 Beta-ketoacyl synthases active site,SC2C4.05c, probable type I polyketide synthase (fragment), len: >1107 aa; similar to C-terminal region of TR:O33955 (EMBL:U78289) Streptomyces fradiae tylactone synthase module 3 TylG, 1864 aa; fasta scores: opt: 2105 z-score: 2202.2 E(): 0; 46.0% identity in 1161 aa overlap. Also highly similar, in this same cosmid, to SC2C4.03c and SC2C4.04c. Contains Pfam matches to entries PF00698 Acyl_transf, Acyl transferase domain and PF00550 pp-binding, Phosphopantetheine attachment site and matches to Prosite entries PS00216 Sugar transport proteins signature 1 and PS00012 Phosphopantetheine attachment site 4 3 9 8 [Reporter]SCO2987 (14L18)_PCR/5727 [Gene]SCO2987/10437 NC_003888 SCE50.15, possible regulatory protein, len: 168 aa; similar to SW:HPCR_ECOLI (EMBL:S56952) Escherichia coli homoprotocatechuate degradative operon repressor HpcR, 148 aa; fasta scores: opt: 151 z-score: 195.2 E(): 0.002; 27.9% identity in 136 aa overlap. Contains Pfam match to entry PF01047 MarR, MarR family 4 2 21 3 [Reporter]SCO4896 (20H3)_PCR/3970 [Gene]SCO4896/7217 NC_003888 2SCK8.22c, probable transport integral membrane protein, len: 436 aa; similar to TR:AAG56583 (EMBL:AE005383) Escherichia coli O157:H7 putative transport protein YnfM, 417 aa; fasta scores: opt: 971 z-score: 995.9 E(): 0; 38.8% identity in 418 aa overlap. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter and matches to Prosite entries PS00216 Sugar transport proteins signature 1 and PS00017 ATP/GTP-binding site motif A (P-loop. Also contains possible hydrophobic membrane spanning regions) 4 3 9 7 [Reporter]SCO1723 (15H18)_PCR/5726 [Gene]SCO1723/10435 NC_003888 SCI11.12c, possible RNA polymerase sigma factor, len: 185 aa; weakly similar to RNA polymerase sigma factors e.g. SW:CARQ_MYXXA (EMBL:X71062), CarQ, Myxococcus xanthus RNA polymerase sigma factor (174 aa), fasta scores; opt: 233 z-score: 295.7 E(): 3.9e-09, 29.3% identity in 167 aa overlap. Similar to many putative sigma factors e.g. TR:O53730 (EMBL:AL021932), SigK, Mycobacterium tuberculosis probable RNA polymerase sigma factor (187 aa) (51.4% identity in 173 aa overlap). Similar to others from S.coelicolor e.g. TR:O54163 (EMBL:AL021411) S. coelicolor possible sigma factor (200 aa) (30.2% identity in 182 aa overlap). Contains Pfam match to entry PF00776 Sigma70_ECF, Sigma-70 factor (ECF subfamily) 4 2 21 2 [Reporter]SCO7477 (20P23)_PCR/3969 [Gene]SCO7477/7215 NC_003888 SCBAC17A6.10, possible membrane protein, len: 497 aa: no significant database matches. Contains a possible hydrophobic membrane spanning region and Prosite match to PS00216 Sugar transport proteins signature 1. 4 3 9 6 [Reporter]SCO3658 (16D18)_PCR/5725 [Gene]SCO3658/10433 NC_003888 SCH10.36, probable aspartate aminotransferase, len: 399 aa; similar to many e.g. TR:Q56232 (EMBL:D38459) Thermus aquaticus aspartate aminotransferase (385 aa), fasta scores; opt: 731 z-score: 825.9 E(): 0, 36.5% identity in 392 aa overlap. Note that there are three possible start codons. Similar to many putative aminotransferases from Streptomyces coelicolor e.g. TR:O54170 (EMBL:AL021411) SC7H1.11, putative aminotransferase (382 aa) (36.6% identity in 331 aa overlap). Contains Pfam match to entry PF00155 aminotran_1, Aminotransferases class-I, score 159.80, E-value 4.6e-44 4 2 20 22 [Reporter]SCO1353 (1P19)_PCR/3967 [Gene]SCO1353/7213 NC_003888 2SCG61.35, possible transcriptional regulator, len: 343 aa; similar to many from proposed regulators from streptomyces e.g. TR:Q9ZBT5 (EMBL:AL034446) putative regulatory protein from Streptomyces coelicolor (327 aa) fasta scores; opt: 445, z-score: 498.6, E(): 2.7e-20, 31.7% identity in 325 aa overlap. Contains helix-turn-helix motif (residue 298-319) 4 3 9 5 [Reporter]SCO3216 (17P14)_PCR/5724 [Gene]SCO3216/10431 NC_003888 SCE8.09, probable integral membrane ATPase, len: 796 aa; member of a large family including SW:Y1B2_MYCTU putative cation-transporting ATPase from Mycobacterium tuberculosis (797 aa) fasta scores; opt: 2741, z-score: 2896.9, E(): 0, (56.8% identity in 791 aa overlap). Contains two Pfam matches to entry PF00122 E1-E2_ATPase, E1-E2 ATPases, score 109.40, E-value 9.7e-30 and score 155.80, E-value 7.3e-43, and Prosite match to PS00154 E1-E2 ATPases phosphorylation site. 4 2 20 21 [Reporter]SCO7432 (2L19)_PCR/3966 [Gene]SCO7432/7211 NC_003888 SC6D11.28c, mprA2, secreted extracellular small neutral protease (EC 3.4.24.-), len: 227 aa. Identical to Streptomyces lividans SW:SNPA_STRLI(EMBL:M81703) extracellular small neutral protease precursor, SnpA (227 aa), fasta scores opt: 1483 z-score: 1546.5 E(): 0 100.0% identity in 227 aa overlap. Also highly similar to Streptomyces coelicolor SW:SNPA_STRCO(EMBL:Z11929) extracellular small neutral protease precursor, MprA (215 aa), fasta scores opt: 854 z-score: 896.8 E():0 65.6% identity in 218 aa overlap. Contains a possible N-terminal signal sequence. 4 2 20 20 [Reporter]SCO7663 (3H19)_PCR/3965 [Gene]SCO7663/7209 NC_003888 SC10F4.36c, conserved hypothetical protein, len: 94 aa; similar to TR:O05862 (EMBL:Z95120) Mycobacterium tuberculosis hypothetical 11.0 kDa protein MTCY07D11.22c, 101 aa; fasta scores: opt: 180 z-score: 238.5 E(): 9.5e-06; 34.8% identity in 92 aa overlap 4 3 9 3 [Reporter]SCP1.286 (19H14)_PCR/5722 [Gene]SCP1.286/10427 NC_003888 SCP1.286, unknown, len: 84aa; similar to SCP1.225 hypothetical protein (previously sequenced as TR:Q9JN71 (EMBL:AJ276673)) from this plasmid fasta scores: E(): 1.1, 40.7% id in 81 aa. 4 2 20 19 [Reporter]SCO5614 (4D19)_PCR/3964 [Gene]SCO5614/7207 NC_003888 SC2E1.31, possible prophage transcriptional regulator, len: 63 aa; similar to the N-terminus of many e.g. TR:E264367 (EMBL:X87420) Salmonella typhimurium bacteriophage ES18 gene C2 (216 aa), fasta scores; opt: 133 z-score: 207.9E(): 0.00024, 35.8% identity in 53 aa overlap. Contains probable helix-turn-helix motif at aa 17 to 38 (Score 1786, +5.27 SD) 4 3 9 2 [Reporter]SCO0820 (20P10)_PCR/5721 [Gene]SCO0820/10425 NC_003888 SCF43A.10c, unknown, len: 119 aa; unknown function, probable CDS suggested by GC frameplot, positional base preference and amino acid composition 4 2 20 18 [Reporter]SCO7687 (5P15)_PCR/3963 [Gene]SCO7687/7205 NC_003888 SC4C2.22, probable thioesterase, len: 277 aa; similar to SW:PABT_STRGR (EMBL:M93058) Streptomyces griseus probable cadicidin biosynthesis thioesterase (EC 3.1.2.-), 361 aa; fasta scores: opt: 638 z-score: 760.5 E(): 0; 40.4% identity in 277 aa overlap. Contains Pfam match to entry PF00975 Thioesterase, Thioesterase domain 4 3 8 22 [Reporter]SCO4220 (1P6)_PCR/5719 [Gene]SCO4220/10423 NC_003888 2SCD46.34c, conserved hypothetical protein, len: 139aa; similar to others from actinomycetes eg. TR:CAB89437 (EMBL:AL354048) hypothetical protein from Streptomyces coelicolor (145 aa) fasta scores; opt: 105, z-score: 143.5, E(): 1.6, 37.0% identity in 146 aa overlap. 4 2 20 17 [Reporter]SCO2189 (6L15)_PCR/3962 [Gene]SCO2189/7203 NC_003888 SC5F7.12c, possible acetyltransferase, len: 169 aa; similar to SW:PTCR_ALCFA phosphinothricin acetyltransferase from Alcaligenes faecalis (197 aa) fasta scores; opt: 178, z-score: 226.5, E(): 2.8e-05, (36.0% identity in 136 aa overlap) and TR:O33583 (EMBL:S60706) nourseothricin acetyltransferase from Streptomyces noursei (190 aa) fasta scores; opt: 140, z-score: 180.8, E(): 0.0098, (29.3% identity in 150 aa overlap). Contains Pfam match to entry PF00583 Acetyltransf, Acetyltransferase (GNAT) family. 4 3 8 20 [Reporter]SCO4779 (3H6)_PCR/5717 [Gene]SCO4779/10421 NC_003888 SCD63.11, pkaJ, serine/threonine protein kinase, len: 548 aa; N-terminal region similar to TR:CAB50939 (EMBL:AL096849) Streptomyces coelicolor putative protein serine/threonine kinase SCI11.13, 550 aa; fasta scores: opt: 1006 z-score: 815.3 E(): 0; 56.9% identity in 297 aa overlap and to TR:AAD47061 (EMBL:AF159501) Myxococcus xanthus Pkn4, 643 aa; fasta scores: opt: 639 z-score: 521.3 E(): 1.4e-21; 43.6% identity in 273 aa overlap and C-terminal similar to TR:Q9WTP4 (EMBL:AB019577) Mus musculus UNC-51-like kinase (ULK) 2, Ulk2, 1037 aa; blastp socres: Sum P(2) = 2.6e-24 Identities = 89/289 (30%), Positives = 144/289 (49%). Contains Pfam match to entry PF00069 pkinase, Eukaryotic protein kinase domain and matches to Prosite entries PS00107 Protein kinases ATP-binding region signature and PS00108 Serine/Threonine protein kinases active-site signature 4 2 20 16 [Reporter]SCO6173 (7H15)_PCR/3961 [Gene]SCO6173/7201 NC_003888 SC6C5.09, probable permease, len: 485aa; similar to many eg. SW:Y326_METJA hypothetical protein from Methanococcus jannaschii (436 aa) fasta scores; opt: 517, z-score: 1019.3, E(): 0, (37.2% identity in 454 aa overlap). Contains Pfam match to entry PF00860 xan_ur_permease, Xanthine /uracil permeases family, score 43.50, E-value 4.9e-09. 4 3 8 9 [Reporter]SCO5014 (13L18)_PCR/5706 [Gene]SCO5014/10401 NC_003888 SCK15.16, possible secreted protein, len: 192 aa. Contains possible N-terminal region signal peptide sequence 4 2 10 15 [Reporter]SCO5577 (8L3)_PCR/3740 [Gene]SCO5577/6799 NC_003888 SC7A1.21, possible chromosome associated protein, len: 1186aa; similar to many hypotheticals eg. SW:Y06B_MYCTU hypothetical protein from Mycobacterium tuberculosis (1289 aa) fasta scores; opt: 2637, z-score: 2806.3, E(): 0, (53.1% identity in 1205 aa overlap) with weak similarity to a family of chromosome associated proteins e.g. SW:SCII_CHICK chromosome scaffold protein SCII from chicken (Gallus gallus) (1189 aa) opt: 370, z-score: 383.3, E(): 4.7e-14, (22.6% identity in 1246 aa overlap). Contains PS00017 ATP /GTP-binding site motif A (P-loop). Also contains 2 probable coiled coil regions from 162 to 492 and from 654 to 1041. 4 2 10 14 [Reporter]SCO7711 (9H3)_PCR/3739 [Gene]SCO7711/6797 NC_003888 SC8D11.02, possible two component system sensor kinase, len: 509 aa; similar to TR:Q53893 (EMBL:U51332) Streptomyces coelicolor AbsA1, 571 aa; fasta scores: opt: 852 z-score: 873.7 E(): 0; 42.1% identity in 489 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 10 13 [Reporter]SCO5117 (10D3)_PCR/3738 [Gene]SCO5117/6795 NC_003888 SC9E12.02, possible peptide transport system secreted peptide-binding protein, len: 544 aa; similar to SW:OPPA_BACSU (EMBL:X56347) Bacillus subtilis oligopeptide-binding protein OppA precursor, 545 aa; fasta scores: opt: 648 z-score: 642.6 E(): 2.5e-28; 26.1% identity in 552 aa overlap and to TR:P72407 (EMBL:U68036) Streptomyces coelicolor SpoOKA homologue BldKB, 602 aa; blastp scores: Score = 165 (58.1 bits), Expect = 2.9e-10, Sum P(2) = 2.9e-10; Identities = 116/502 (23%), Positives = 202/502 (40%). Contains 2x Pfam matches to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5 and correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Also contains possible N-terminal region signal peptide sequence 4 2 10 12 [Reporter]SCO2689 (10P23)_PCR/3737 [Gene]SCO2689/6793 NC_003888 SCC61A.10, possible membrane protein, len: 375aa; contains possible membrane-spanning hydrophobic regions. 4 2 10 11 [Reporter]SCO4432 (11L23)_PCR/3736 [Gene]SCO4432/6791 NC_003888 SCD6.10, unknown, len: 224 aa 4 2 10 10 [Reporter]SCO3502 (12H23)_PCR/3735 [Gene]SCO3502/6789 NC_003888 SCE134.03c, possible 3-oxoacyl-[acyl carrier protein] reductase, len: 255 aa; similar to many e.g. SW:FABG_BACSU 3-oxoacyl-[acyl carrier protein] reductase from Bacillus subtilis (246 aa) fasta scores; opt: 522, z-score: 518.3, E(): 1.5e-21, (38.1% identity in 247 aa overlap). Contains Pfam matches to entries PF00678 adh_short_C2, Short chain dehydrogenase/reductase C-terminus and PF00106 adh_short, short chain dehydrogenase. 4 2 10 9 [Reporter]SCO0947 (13D23)_PCR/3734 [Gene]SCO0947/6787 NC_003888 SCM11.02, possible integral membrane protein, len: 470 aa; similar to TR:P96418 (EMBL:Z92669) Mycobacterium tuberculosis hypothetical 54.8 kD protein MTCY08D5.31c, 482 aa; fasta scores: opt: 2112 z-score: 2527.5 E(): 0; 61.9% identity in 472 aa overlap and to TR:O69633 (EMBL:AL022121) Mycobacterium tuberculosis putative peptide transport system permease; fasta scores: opt: 122 z-score: 149.8 E(): 0.58; 27.7% identity in 166 aa overlap 4 2 10 8 [Reporter]SCO2959 (14P19)_PCR/3733 [Gene]SCO2959/6785 NC_003888 SCE59.18c, probable nitrate extrusion protein, len: 452 aa; similar to SW:NARK_ECOLI (EMBL:X15996) Escherichia coli nitrite extrusion protein 1 (nitrite facilitator 1) NarK, 463 aa; fasta scores: opt: 526 z-score: 590.8 E(): 1.7e-25; 39.7% identity in 451 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 10 7 [Reporter]SCO0179 (15L19)_PCR/3732 [Gene]SCO0179/6783 NC_003888 SCJ1.28c,possible zinc-containing dehydrogenase, len: 356 aa. Similar to many including: Thermoanaerobacter brockii SW:ADH_THEBR (EMBL; X64841) NADP-dependent alcohol dehydrogenase (EC 1.1.1.2) (352 aa), fasta scores opt: 556 z-score: 601.1 E(): 4e-26 33.7% identity in 338 aa overlap and Halobacterium volcanii TR:O34179 (EMBL; U95372) dehydrogenase (389 aa), fasta scores opt: 715 z-score: 770.1 E(): 0 37.0% identity in 351 aa overlap. Contains a Pfam match to entry PF00107 adh_zinc, Zinc-binding dehydrogenases and a PS00059 Zinc-containing alcohol dehydrogenases signature 4 2 10 6 [Reporter]SCO0394 (16H19)_PCR/3731 [Gene]SCO0394/6781 NC_003888 SCF62.20, unknown, len: 215 aa 4 2 10 5 [Reporter]SCO3741 (17D19)_PCR/3730 [Gene]SCO3741/6779 NC_003888 SCH22A.19c, probable response regulator, len: 256 aa; similar to TR:P74922 (EMBL:U67196) Thermotoga maritima DNA-binding response regulator DrrA, 239 aa; fasta scores: opt: 664 z-score: 686.2 E(): 8.4e-31; 45.3% identity in 234 aa overlap and to SW:AFQ1_STRCO (EMBL:D10654) Streptomyces coelicolor transcriptional regulatory protein AfsQ1, 225 aa; fasta scores: opt: 630 z-score: 652.1 E(): 6.7e-29; 47.1% identity in 221 aa overlap. Contains Pfam matches to entries PF00072 response_reg, Response regulator receiver domain and PF00486 trans_reg_C, Transcriptional regulatory protein, C terminal. Contains also possible N-terminal region signal peptide sequence 4 2 10 4 [Reporter]SCO6098 (18P15)_PCR/3729 [Gene]SCO6098/6777 NC_003888 SCBAC1A6.22c, cysD, sulfate adenylyltransferase subunit 2, len: 312 aa; highly similar to SW:MMCV_STRLA (EMBL:AF127374) Streptomyces lavendulae sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) MmcV, 319 aa; fasta scores: opt: 1734 z-score: 2027.7 E(): 0; 81.6% identity in 309 aa overlap and to SW:CYSD_ECOLI (EMBL:M74586) Escherichia coli sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) CysD, 302 aa; fasta scores: opt: 909 z-score: 1066.6 E(): 0; 47.7% identity in 298 aa overlap. Contains Pfam match to entry PF01507 PAPS_reduct, Phosphoadenosine phosphosulfate reductase family 4 2 10 3 [Reporter]SCO1378 (19L15)_PCR/3728 [Gene]SCO1378/6775 NC_003888 SC10A9.20c, probable glycine dehydrogenase, len: 961 aa; similar to SW:GCSP_ECOLI (EMBL:L20872) Escherichia coli glycine dehydrogenase [decarboxylating] (EC 1.4.4.2) GcvP, 956 aa; fasta scores: opt: 3621 z-score: 3847.9 E(): 0; 56.5% identity in 960 aa overlap. Contains Pfam match to entry PF02347 GDC-P 4 2 10 2 [Reporter]SCO5110 (20D15)_PCR/3727 [Gene]SCO5110/6773 NC_003888 SCBAC31E11.06, possible lipoprotein, len: 771 aa; similar to SW:YC80_MYCTU (EMBL:Z77137) Mycobacterium tuberculosis hypothetical 63.5 kDa protein MTCY50.02, 591 aa; fasta scores: opt: 565 Z-score: 501.6 bits: 103.2 E(): 2.5e-20; 26.190% identity in 756 aa overlap. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 2 9 22 [Reporter]SCO2798 (1D11)_PCR/3725 [Gene]SCO2798/6771 NC_003888 2SCC13.06, probable cellobiose hydrolase, len: 479 aa; highly similar to TR:Q59976 (EMBL:Z29625) Streptomyces sp. beta-glucosidase (EC 3.2.1.21) (cellobiose) Bgl3, 479 aa; fasta scores: opt: 2214 z-score: 2511.6 E(): 0; 65.5% identity in 472 aa overlap. Contains Pfam match to entry PF00232 Glyco_hydro_1, Glycosyl hydrolase family 1 and matches to Prosite entries PS00653 Glycosyl hydrolases family 1 N-terminal signature and PS00572 Glycosyl hydrolases family 1 active site 4 2 9 21 [Reporter]SCO0190 (2P7)_PCR/3724 [Gene]SCO0190/6769 NC_003888 SCJ12.02c, crtT, probable methyltransferase, len: 246 aa. Shares a high level of sequence similarity with Streptomyces griseus TR:P72450 (EMBL:X95596) methyltransferase (242 aa), fasta scores opt: 984 z-score: 1164.2 E(): 0 65.0% identity in 234 aa overlap. Contains a Pfam match to entry PF01209 Ubie_methyltran, ubiE/COQ5 methyltransferase family 4 2 9 20 [Reporter]SCO4715 (3L7)_PCR/3723 [Gene]SCO4715/6767 NC_003888 SCD31.40, rpsN, 30S ribosomal protein S14, len: 61 aa; highly similar to SW:RS14_BACST Bacillus stearothermophilus 30S ribosomal protein S14 RpsN, 61 aa; fasta scores: opt: 335 z-score: 483.7 E(): 1.6e-19; 68.9% identity in 61 aa overlap. Contains Pfam match to entry PF00253 Ribosomal_S14, Ribosomal protein S14p/S29e and match to Prosite entry PS00527 Ribosomal protein S14 signature 4 2 9 19 [Reporter]SCO5521 (4H7)_PCR/3722 [Gene]SCO5521/6765 NC_003888 SC1C2.02, questionable ORF, len: 43 aa 4 2 9 18 [Reporter]SCO5731 (5D7)_PCR/3721 [Gene]SCO5731/6763 NC_003888 SC3C3.17c, possible secreted serine protease, len: 450 aa; similar in part to many e.g. SUBT_BACSU subtilisin E precursor (381 aa), fasta scores; opt: 321 z-score: 359.3 E(): 9.4e-13, 32.3% identity in 294 aa overlap. Also similar to downstream serine protease, SC3C3.08 (E(): 7.1e-07, 35.0% identity in 383 aa overlap). Contains N-terminal signal sequence, possible transmembrane domain at C-terminus and Pfam match to entry PF00082 subtilase, Subtilase family of serine proteases, score 151.00, E-value 2.1e-41 4 2 9 17 [Reporter]SCO6670 (6P3)_PCR/3720 [Gene]SCO6670/6761 NC_003888 SC5A7.20, probable glucosidase, len: 441 aa; similar to e.g. CELF_ECOLI 6-phospho-beta-glucosidase (450 aa), fasta scores; opt: 537 z-score: 628.7 E(): 8.8e-28, 31.8% identity in 456 aa overlap 4 2 9 16 [Reporter]SCO3613 (7L3)_PCR/3719 [Gene]SCO3613/6759 NC_003888 SC66T3.24c, possible RNA polymerase sigma factor, len: 190 aa; weakly similar to many e.g. SW:RPOE_STRCO (EMBL:L29636), AigE, S.coelicolor RNA polymerase sigma-E factor (176 aa), fasta scores; opt: 236 z-score: 274.0 E(): 6.1e-08, 30.9% identity in 162 aa overlap. The start site is uncertain as there is a second possible start site at codon 7 4 2 9 15 [Reporter]SCO6685 (8H3)_PCR/3718 [Gene]SCO6685/6757 NC_003888 SC5A7.35c, ramR, two-component system response regulator, len: >41 aa. Identical to TR:Q53820 (EMBL:U03771) potential response regulator for aerial mycelium production RamR (202 aa), and similar to many e.g. Streptomyces peucetius TR:Q54821 (EMBL:L37338) daunorubicin biosynthesis regulatory protein dnrN (202 aa), fasta scores; opt: 173 z-score: 382.4 E(): 4.6e-14, 65.0% identity in 40 aa overlap,SC6G3.01c, ramR, probable two-component system response regulator, partial CDS, len: >201 aa; previously sequenced as TR:Q53820 (EMBL:U03771), RamR, potential response regulator for aerial mycelium production. Similar to many two-component system transcriptional regulators e.g. TR:Q07640 (EMBL:AB006206), AfmR, Streptomyces griseus transcriptional regulator involved in aerial mycelium formation (210 aa), fasta scores; opt: 466 z-score: 545.2 E(): 4.9e-23, 42.6% identity in 202 aa overlap. An alternative start codon is present at codon 4. Contains probable helix-turn-helix motif at aa 158-179 (Score 1876, +5.58 SD). Contains Pfam match to PF00196 GerE, Bacterial regulatory proteins, luxR family 4 2 9 14 [Reporter]SCO5670 (9D3)_PCR/3717 [Gene]SCO5670/6755 NC_003888 SC8B7.08c, probable polyamine ABC-transporter integr al membrane protein, len: 266 aa; similar to many e.g. POTC _ECOLI spermidine/putrescine transport system permease (264 aa), fasta scores; opt: 579 z-score: 718.8 E(): 8.8e-33, 3 5.5% identity in 251 aa overlap. Contains Pfam match to ent ry PF00528 BPD_transp, Binding-protein-dependent transport systems inner membrane component, score 23.00, E-value 0.00 7 4 2 9 13 [Reporter]SCO6465 (9P23)_PCR/3716 [Gene]SCO6465/6753 NC_003888 SC1A11.02c, partial CDS, unknown, len: 134aa; N-terminal region similar to TR:O27370 (EMBL:AE000895) proposed mutator MutT protein from Methanobacterium thermoautotrophicum (135 aa) fasta scores; opt: 148, z-score: 219.8, E(): 5.9e-05, (43.5% identity in 62 aa overlap).,SC9C7.01c, partial CDS, unknown, len: 49aa; overlapping extreme N-terminal region of partial CDS, SC1A11.2c from Streptomyces coelicolor cosmid 1A11. 4 2 9 12 [Reporter]SCO2683 (10L23)_PCR/3715 [Gene]SCO2683/6751 NC_003888 SCC61A.04c, possible single-strand DNA-binding protein, len: 156 aa; similar to many eg. SW:P71711 (SSB_MYCTU) single-strand binding protein from Mycobacterium tuberculosis (164 aa) fasta scores; opt: 259, z-score: 301.8, E(): 2.4e-09, 35.5% identity in 141 aa overlap and TR:Q9X8U3 (EMBL:AL049826) putative single-strand binding protein from Streptomyces coelicolor (199 aa) fasta scores; opt: 261, z-score: 302.7, E(): 2.1e-09, 42.5% identity in 120 aa overlap. Contains Pfam match to entry PF00436 SSB, Single-strand binding protein family. 3 1 14 18 [Reporter]SCO5464 (5G12)_PCR/1311 [Gene]SCO5464/2399 NC_003888 SC3D11.21, possible calcium-binding protein, len: 70aa; similar to many eukaryotic calcium-binding proteins eg. TR:Q42470 (EMBL:D63153) calcium-binding protein from Brassica rapa (Turnip), and Brassica napus (Rape) (79 aa) fasta scores; opt: 153, z-score: 214.7, E(): 0.00015, 45.3% identity in 64 aa overlap and SW:P02599 (CALM_DICDI) calmodulin from Dictyostelium discoideum (Slime mold) (151 aa) fasta scores; opt: 155, z-score: 213.0, E(): 0.00019, 43.3% identity in 60 aa overlap. Contains Pfam match to entry PF00036 efhand, EF hand and two Prosite matches to PS00018 EF-hand calcium-binding domain. 4 2 9 11 [Reporter]SCO4680 (11H23)_PCR/3714 [Gene]SCO4680/6749 NC_003888 SCD31.05c, possible DNA-binding protein, len: 296 aa; similar to TR:CAB53273 (EMBL:AL109972) Streptomyces coelicolor putative DNA binding protein SCJ9A.12c, 279 aa; fasta scores: opt: 782 z-score: 910.0 E(): 0; 46.1% identity in 271 aa overlap 4 2 9 10 [Reporter]SCO3393 (12D23)_PCR/3713 [Gene]SCO3393/6747 NC_003888 SCE126.11, possible integral membrane protein, len: 151aa; similar to other hypotheticals eg. TR:P96615 (EMBL:AB001488) hypothetical protein from Bacillus subtilis (159 aa) fasta scores; opt: 208, z-score: 264.3, E(): 2.1e-07, (30.3% identity in 145 aa overlap). Contains a possible membrane spanning hydrophobic region. 4 2 9 9 [Reporter]SCO5173 (13P19)_PCR/3712 [Gene]SCO5173/6745 NC_003888 SCP8.36, hypothetical protein, len: 559 aa; low similarity to TR:Q9XAG2 (EMBL:AL079356) Streptomyces coelicolor possible membrane protein SC6G9.20, 303 aa; fasta scores: opt: 452 z-score: 362.9 E(): 9.5e-13; 34.1% identity in 258 aa overlap 4 2 9 8 [Reporter]SCO1463 (14L19)_PCR/3711 [Gene]SCO1463/6743 NC_003888 SCL6.20c, probable transcriptional regulator, len: 360 aa; similar to SW:DEOR_BACSU (EMBL:X82174) Bacillus subtilis deoxyribonucleoside regulator DeoR, 313 aa ;fasta scores: opt: 363 z-score: 408.8 E(): 2.4e-15; 28.0% identity in 307 aa overlap. Contains possible helix-turn-helix motif at residues 54..75 (+4.15 SD) 4 2 9 7 [Reporter]SCO3871 (15H19)_PCR/3710 [Gene]SCO3871/6741 NC_003888 SCH18.08, possible decarboxylase, len: 136 aa; similar to TR:O26336 (EMBL:AE000810) Methanobacterium thermoautotrophicum gamma-carboxymuconolactone decarboxylase Mth234, 125 aa; fasta scores: opt: 231 z-score: 310.2 E(): 8.3e-10; 38.3% identity in 107 aa overlap 3 1 14 12 [Reporter]SCO5282 (11O4)_PCR/1305 [Gene]SCO5282/2389 NC_003888 SCCB12.06c, possible two-component system sensor kinase, len: 375 aa; similar to SW:AFQ2_STRCO (EMBL:D10654) Streptomyces coelicolor sensor protein AfsQ2, 535 aa; fasta scores: opt: 344 z-score: 382.3 E(): 7.9e-14; 32.5% identity in 335 aa overlap. Contains Pfam matches to entries PF00672 DUF5, HAMP domain and PF00512 signal, Histidine kinase. Also contains possible hydrophobic membrane spanning regions 4 2 9 5 [Reporter]SCO0868 (17P15)_PCR/3708 [Gene]SCO0868/6739 NC_003888 SCM1.01c, possible regulatory protein, len: 147 aa. Similar to Streptomyces fradiae TR:Q9Z9I5 (EMBL:AB024056) regulatory protein (139 aa), fasta scores opt: 166 z-score: 217.5 E(): 9.1e-05 30.9% identity in 123 aa overlap and Streptomyces coelicolor A3(2) TR:CAB55834 (EMBL:AJ249581) putative regulator of sig15 (170 aa), fasta scores opt: 157 z-score: 205.1 E(): 0.00044 32.8% identity in 119 aa overlap. 4 2 9 4 [Reporter]SCO1659 (18L15)_PCR/3707 [Gene]SCO1659/6737 NC_003888 SCI52.01, glpF, probable glycerol uptake facilitator protein, len: 264 aa; N-terminal region identical to previously sequenced SW:GLPF_STRCO (EMBL:X14188) Streptomyces coelicolor probable glycerol uptake facilitator protein (fragment) GlpF or GylA, 80 aa, and similar to SW:GLPF_BACSU (EMBL:M99611) Bacillus subtilis glycerol uptake facilitator protein GlpF, 274 aa; fasta scores: opt: 520 Z-score: 611.2 bits: 120.8 E(): 1.8e-26; 51.163% identity in 258 aa overlap. Contains Pfam match to entry PF00230 MIP, Major intrinsic protein and match to Prosite entry PS00221 MIP family signature. Also contains possible hydrophobic membrane spanning regions 4 2 9 3 [Reporter]SCO1677 (19H15)_PCR/3706 [Gene]SCO1677/6735 NC_003888 SCI52.19, conserved hypothetical protein, len: 124 aa; similar to TR:BAB35158 (EMBL:AP002556) Escherichia coli O157:H7 hypothetical 17.0 kDa protein ECS1735, 152 aa; fasta scores: opt: 397 Z-score: 489.0 bits: 96.3 E(): 1.2e-19; 52.033% identity in 123 aa overlap 4 2 8 22 [Reporter]SCO4369 (1P7)_PCR/3703 [Gene]SCO4369/6733 NC_003888 2SCD52.01, hypothetical protein (fragment), len: >312 aa; similar to N-terminal region of TR:CAB95880 (EMBL:AL359988) Streptomyces coelicolor hypothetical 26.1 kDa protein (fragment) SCD10.01, 261 aa; fasta scores: opt: 232 z-score: 233.3 E(): 1.7e-05; 32.0% identity in 225 aa overlap,SCD10.01, unknown (fragment), len: >261 aa. High content in glycine and aspartate amino acid residues. Contains 2x Pfam match to entry PF01839 FG-GAP, FG-GAP repeat 4 2 8 21 [Reporter]SCO0560 (2L7)_PCR/3702 [Gene]SCO0560/6731 NC_003888 SCF73.07c, cpeB, catalase/peroxidase, len: 740 aa; highly similar to TR:O87864 (EMBL:Y14317) Streptomyces reticuli catalase/peroxidase (EC 1.11.1.6) (740 aa), fasta scores; opt: 4603 z-score: 5243.8 E(): 0, 90.5% identity in 739 aa overlap, and similar to many e.g. CATA_MYCBO peroxidase/catalase (EC 1.11.1.6) (740 aa), fasta scores; opt: 3432 z-score: 3909.0 E(): 0, 66.7% identity in 744 aa overlap. Contains PS00436 Peroxidases active site signature, PS00435 Peroxidases proximal heme-ligand signature, PS00017 ATP/GTP-binding site motif A (P-loop), and Pfam match to entry PF00141 peroxidase 3 1 14 7 [Reporter]SCO1584 (15K24)_PCR/1300 [Gene]SCO1584/2379 NC_003888 SCI35.06, unknown, len: 152 aa 4 2 8 19 [Reporter]SCO6050 (4D7)_PCR/3700 [Gene]SCO6050/6729 NC_003888 SC1B5.10c, probable lipoprotein, len: 289 aa; contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site 4 2 8 18 [Reporter]SCO5893 (5P3)_PCR/3699 [Gene]SCO5893/6727 NC_003888 SC3F7.13, redK, probable oxidoreductase, len: 347 aa; similar to many eukaryotic steroid dehydrogenases eg. TR:Q62878 (EMBL:L17138) 3 hydroxysteroid dehydrogenase from Rattus norvegicus (373 aa), fasta scores; opt: 103 z-score: 378.9 E(): 6.6e-14, 30.4% identity in 355 aa overlap; and to Mycobacterium tuberculosis putative dehydrogenase TR:O06373 (EMBL:Z95436) MTCY15C10.18 (314 aa), fasta scores; opt: 204 z-score: 417.9 E(): 4.4e-16, 27.7% identity in 339 aa overlap 4 2 8 17 [Reporter]SCO6666 (6L3)_PCR/3698 [Gene]SCO6666/6725 NC_003888 SC5A7.16c, probable integral membrane protein len: 705 aa; similar to many hypothetical membrane proteins e.g. M. tuberculosis TR:E1299723 (EMBL:AL021928) MMPL3 (944 aa), fasta scores; opt: 482 z-score: 907.8 E(): 0, 33.3% identity in 744 aa overlap 4 2 8 16 [Reporter]SCO0317 (7H3)_PCR/3697 [Gene]SCO0317/6723 NC_003888 SC5G9.26c, probable transmembrane transport protein, len: 420 aa; shows weak similarity to many efflux pumps e.g. SW:CMLR_STRLI (EMBL:X59968), cmlR, Streptomyces lividans chloramphenicol resistance protein (392 aa), fasta scores; opt: 276 z-score: 292.9 E(): 6e-09, 27.3% identity in 407 aa overlap. Contains hydrophobic, possible membrane-spanning regions 4 2 8 15 [Reporter]SCO2159 (8D3)_PCR/3696 [Gene]SCO2159/6721 NC_003888 SC6G10.32, unknown, len: 69aa; 3 1 14 2 [Reporter]SCO7483 (20C20)_PCR/1295 [Gene]SCO7483/2369 NC_003888 SCBAC17A6.16, conserved hypothetical protein, len: 135aa: similar to many eg. TR:BAB48518 (EMBL:AP002996) hypothetical protein MLL1058 from Rhizobium loti (148 aa) fasta scores; opt: 285, Z-score: 348.9, 39.850% identity (41.732% ungapped) in 133 aa overlap. Contains Pfam match to entry PF01042 UPF0076, YjgF family. 4 2 8 14 [Reporter]SCO6583 (8P23)_PCR/3695 [Gene]SCO6583/6719 NC_003888 SC8A6.04c, probable transferase, len: 410 aa; highly similar to TR:O06644 (EMBL:U82167) formyl-CoA transferase from Oxalobacter formigenes (428 aa), fasta scores; opt: 95 4 z-score: 1732.0 E(): 0, 50.7% identity in 428 aa overlap. Also similar to many dehydratases e.g. CAIB_ECOLI L-carnit ine dehydratase (EC 4.2.1.89) (405 aa), fasta scores; opt: 285 z-score: 317.7 E(): 1.9e-10, 26.2% identity in 427 aa o verlap 4 2 8 13 [Reporter]SCO1507 (9L23)_PCR/3694 [Gene]SCO1507/6717 NC_003888 SC9C5.31c, possible integral membrane protein, len: 240 aa; similar to TR:O69464 (EMBL:AL023635) Mycobacterium leprae putative integral membrane protein MLCB1243.07, 214 aa; fasta scores: opt: 511 z-score: 600.4 E(): 5.7e-26; 36.1% identity in 191 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 8 12 [Reporter]SCO2728 (10H23)_PCR/3693 [Gene]SCO2728/6715 NC_003888 SCC46.13, unknown, len: 132 aa 4 2 8 11 [Reporter]SCO4097 (11D23)_PCR/3692 [Gene]SCO4097/6713 NC_003888 SCD17.01, possible amino acid/metabolite permease, partial CDS, len: >389 aa. Similar to many Eukaryotic permeases e.g. Arabidopsis thaliana TR: O22509 (EMBL; AF019637) putative amino acid or GABA permease (516 aa), fasta scores opt: 827 z-score: 942.8 E(): 0 41.8% identity in 385 aa overlap as well as Escherichia coli SW:YEEF_ECOLI (EMBL; U00009) hypothetical 49.8 KD transport protein in sbcB-hisL intergenic region (454 aa), fasta scores opt: 256 z-score: 295.7 E(): 4.1e-09 24.6% identity in 293 aa overlap. Contains a Pfam match to entry PF00324 aa_permeases, Amino acid permease and multiple possible membrane spanning hydrophobic domains. The 5' region of this CDS is located on cosmid SCD25.,SCD25.33, possible integral membrane protein, partial CDS, len: 156 aa. High level of similarity with Eukaryotic permeases e.g. Arabidopsis thaliana TR:O22509 (EMBL; AF019637) putative amino acid or GABA permease (516 aa), fasta scores opt: 470 z-score: 557.7 E(): 1e-23 53.1% identity in 128 aa overlap and Bacillus subtilis TR:O07576 (EMBL; Y14082) hypothetical 49.7 KD protein (456 aa), fasta scores opt: 128 z-score: 158.4 E(): 0.18 23.3% identity in 133 aa overlap. Contains possible membrane spanning hydrophobic domains. 4 2 8 10 [Reporter]SCO2933 (12P19)_PCR/3691 [Gene]SCO2933/6711 NC_003888 SCE19A.33, probable permease binding-protein component, len: 322 aa; similar to SW:PROX_BACSU (EMBL:U38418), proX, Bacillus subtilis glycine betaine-binding protein precursor (313 aa), fasta scores; opt: 300 z-score: 341.4 E(): 1.1e-11, 27.5% identity in 320 aa overlap and to similar putative permease components. Similar to SC9B1.12 (EMBL:AL049727) S.coelicolor probable secreted substrate-binding protein (320 aa) (30.4% identity in 329 aa overlap). Contains a probable N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. May be part of a multicomponent transporter which includes SCE19A.30, SCE19A.31, SCE19A.32 and SCE19A.33 3 1 13 18 [Reporter]SCO5460 (5C12)_PCR/1289 [Gene]SCO5460/2359 NC_003888 SC3D11.17, possible AbaA-like regulatory protein, len: 220 aa; similar to many from Streptomyces spp. e.g. TRNEW:AAD46514 (EMBL:AF145724) putative regulatory protein from Streptomyces albus (137 aa) fasta scores; opt: 258, z-score: 275.0, E(): 6.6e-08, 45.3% identity in 128 aa overlap and TR:Q53897 (EMBL:X60316) AbaA from Streptomyces coelicolor (192 aa) fasta scores; opt: 226, z-score: 240.9, E(): 5.3e-06, 32.3% identity in 189 aa overlap. 4 2 8 9 [Reporter]SCO0403 (13L19)_PCR/3690 [Gene]SCO0403/6709 NC_003888 SCF51.02c, hypothetical protein, len: 187 aa; similar to various hypothetical proteins, e.g. TR:CAB56690 (EMBL:AL121596) Streptomyces coelicolor SCF51A.38 hypothetical 18.5 KD protein, 169 aa; fasta scores: opt: 176 z-score: 225.6 E(): 3.4e-05; 32.9% identity in 173 aa overlap 4 2 8 8 [Reporter]SCO1173 (14H19)_PCR/3689 [Gene]SCO1173/6707 NC_003888 SCG11A.04, possible transcriptional regulator, len: aa; similar to TR:O31551 (EMBL:Z99108) Bacillus subtilis transcriptional regulator AcoR, 605 aa; fasta scores: opt: 393 z-score: 446.5 E(): 1.7e-17; 34.7% identity in 199 aa overlap 4 2 8 6 [Reporter]SCO3478 (16P15)_PCR/3687 [Gene]SCO3478/6705 NC_003888 SCE65.14c, probable dehydrogenase, len: 344 aa; similar to TR:O50095 (EMBL:AP000006) Pyrococcus horikoshii 307aa long hypothetical phosphoglycerate dehydrogenase PH1387, 307 aa; fasta scores: opt: 687 z-score: 777.1 E(): 0; 40.5% identity in 291 aa overlap and to SW:SERA_METTH (EMBL:AE000870;) Methanobacterium thermoautotrophicum D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) SerA, 525 aa; fasta scores: opt: 664 z-score: 748.1 E(): 0; 39.0% identity in 282 aa overlap. Contains Pfam match to entry PF00389 2-Hacid_DH, D-isomer specific 2-hydroxyacid dehydrogenases and match to Prosite entry PS00670 D-isomer specific 2-hydroxyacid dehydrogenases signature 2 4 2 8 5 [Reporter]SCO3585 (17L15)_PCR/3686 [Gene]SCO3585/6703 NC_003888 SCH66.06, unknown, len: 113aa; 4 2 8 4 [Reporter]SCO1917 (18H15)_PCR/3685 [Gene]SCO1917/6701 NC_003888 SCI7.35c, unknown, len: 197aa; similar to many eg. TR:P95092 (EMBL:Z83866) hypothetical protein from Mycobacterium tuberculosis (202 aa) fasta scores; opt: 398, z-score: 482.3, E(): 1.6e-19, (40.3% identity in 191 aa overlap). 3 1 13 12 [Reporter]SCO5278 (11K4)_PCR/1283 [Gene]SCO5278/2349 NC_003888 SCCB12.02, possible ion chelatase, len: 655 aa; similar to TR: SW:CHLD_SYNP7 (EMBL:AB003135) Synechococcus sp. magnesium-chelatase subunit ChlD (MG-protoporphyrin IX chelatase) ChlD, 677 aa; fasta scores: opt: 304 z-score: 269.4 E(): 1.5e-07; 26.6% identity in 688 aa overlap 4 2 8 3 [Reporter]SCO1674 (19D15)_PCR/3684 [Gene]SCO1674/6699 NC_003888 SCI52.16c, possible secreted protein, len: 259 aa; similar to TR:Q9X7U2 (EMBL:AL049863) Streptomyces coelicolor hypothetical 22.7 kDa protein SC5H1.35c, 237 aa; fasta scores: opt: 566 Z-score: 369.4 bits: 75.8 E(): 5.3e-13; 46.154% identity in 260 aa overlap. High content in glycine amino acid residues. Also contains the following repeats: 2x(SPGV-SGN-V--P--VPVNVCGN--DVVG-LNP--GN-C-N-GGG) and 4x(GG-) and a possible N-terminal region signal peptide sequence 4 2 8 2 [Reporter]SCO5075 (20L11)_PCR/3683 [Gene]SCO5075/6697 NC_003888 SCBAC28G1.01, possible oxidoreductase, previously sequenced as ORF4, len: 167 aa; identical to C-terminal region of previously sequenced TR:Q53929 (EMBL:X62373) Streptomyces coelicolor ORF4, 306 aa; fasta scores: opt: 1029 Z-score: 1146.7 bits: 219.4 E(): 2.9e-56; 95.808% identity in 167 aa overlap. Contains match to Prosite entry PS01162 Quinone oxidoreductase / zeta-crystallin signature,SCBAC20F6.18, ORF4, possible oxidoreductase, len: 170 aa; identical to N-terminal region of the previously sequenced TR:Q53929 (EMBL:X62373) Streptomyces coelicolor ORF 4, 306 aa. Contains match to Prosite entry PS01162 Quinone oxidoreductase / zeta-crystallin signature 4 2 7 22 [Reporter]SCO4224 (1L7)_PCR/3681 [Gene]SCO4224/6695 NC_003888 2SCD46.38c, unknown, len: 421aa; 4 2 7 21 [Reporter]SCO4744 (2H7)_PCR/3680 [Gene]SCO4744/6693 NC_003888 SC6G4.22c, acpS, probable holo-[acyl-carrier protein ] synthase, len: 123 aa; similar to many e.g. ACPS_ECOLI holo-[acyl-carrier protein] synthase (EC 2.7.8.7) (125 aa), fasta scores; opt: 237 z-score: 303.8 E(): 1.1e-09, 38.7% i dentity in 124 aa overlap 4 2 7 19 [Reporter]SCO6513 (4P3)_PCR/3678 [Gene]SCO6513/6691 NC_003888 SC1E6.22c, unknown, len: 373 aa; similar to a hypothetical protein from M. tuberculosis TR:O06183 (EMBL:Z95387) MTCY1A10.03C (374 aa), fasta scores; opt: 425 z-score: 395.8 E(): 9.2e-15, 30.2% identity in 371 aa overlap 4 2 7 18 [Reporter]SCO5888 (5L3)_PCR/3677 [Gene]SCO5888/6689 NC_003888 SC3F7.08, redP, probable 3-oxoacyl-[acyl-carrier-protein] synthase III, len: 335 aa; similar to many eg. FABH_ECOLI P24249 3-oxoacyl-[acyl-carrier-protein] synthase III (EC 2.3.1.41) (beta-ketoacyl-ACP synthase III) (317 aa), fasta scores; opt: 502 z-score: 881.2 E(): 0, 40.4% identity in 319 aa overlap 4 2 7 17 [Reporter]SCO6641 (6H3)_PCR/3676 [Gene]SCO6641/6687 NC_003888 SC4G2.15c, unknown, len: 404 aa; N-terminus is similar to extreme C-terminus of TR:O26587 (EMBL:AE000832) DNA helicase related protein MTH487 (Methanobacterium thermoautotrophicum) (1157 aa), fasta scores; opt: 467 z-score: 553.7 E(): 1.3e-23, 43.7% identity in 174 aa overlap 4 2 7 16 [Reporter]SCO0313 (7D3)_PCR/3675 [Gene]SCO0313/6685 NC_003888 SC5G9.22, probable transmembrane transport protein, len: 449 aa; similar to many transmembrane transporters e.g. SW:DGOT_ECOLI (EMBL:L10328), dgoT, Escherichia coli D-galactonate transporter (445 aa), fasta scores; opt: 332 z-score: 376.2 E(): 1.4e-13, 23.7% identity in 438 aa overlap. Contains hydrophobic, possible membrane-spanning regions. Contains Pfam match to entry PF00083 sugar_tr, Sugar (and other) transporter 4 2 7 15 [Reporter]SCO2131 (7P23)_PCR/3674 [Gene]SCO2131/6683 NC_003888 SC6G10.04, probable long chain fatty acid CoA ligase, len: 598 aa; similar to many e.g. TR:E1359128 (EMBL:AL034443) putative long chain fatty acid CoA ligase from Streptomyces coelicolor (608 aa) fasta scores; opt: 1347, z-score: 1506.7, E(): 0, (51.3% identity in 608 aa overlap) and SW:LCFB_RAT long chain fatty acid CoA ligase from Rattus norvegicus (Rat) (699 aa) fasta scores; opt: 568, z-score: 634.7, E(): 4.7e-28, (30.2% identity in 589 aa overlap). Contains Pfam match to entry PF00501 AMP-binding, AMP-binding enzyme and Prosite match to PS00455 Putative AMP-binding domain signature. 4 2 7 14 [Reporter]SCO2418 (8L23)_PCR/3673 [Gene]SCO2418/6681 NC_003888 SC8A2.06c, possible secreted protein, len: 354 aa; similar to TR:O53968 (EMBL:AL022073) Mycobacterium tuberculosis hypothetical 36.5 kDa protein MTV051.05, 342 aa; fasta scores: opt: 665 z-score: 735.0 E(): 0; 37.6% identity in 343 aa overlap. Contains Pfam match to entry PF02470 mce. Also contains possible N-terminal region signal peptide sequence 4 2 7 13 [Reporter]SCO6070 (9H23)_PCR/3672 [Gene]SCO6070/6679 NC_003888 SC9B1.17c, possible secreted solute binding protein, len: 337aa; similarity to many eg. SW:RBSB_BACSU D-ribose binding protein from Bacillus subtilis (305 aa) fasta scores; opt: 251, z-score: 270.5, E(): 9.6e-08, (26.4% identity in 288 aa overlap). Contains possible N-terminal signal sequence and Prosite match to PS00013 Prokaryotic membrane lipoprotein lipid attachment site. 4 2 7 12 [Reporter]SCO2723 (10D23)_PCR/3671 [Gene]SCO2723/6677 NC_003888 SCC46.08c, probable ABC transporter ATP-binding protein, len: 866 aa; C-terminal region similar to SW:YHCH_BACSU (EMBL:X96983) Bacillus subtilis hypothetical ABC transporter ATP-binding protein 2 in GlpD-CspB intergenic region, YhcH, 305 aa; fasta scores: opt: 597 z-score: 577.6 E(): 9.3e-25; 36.6% identity in 303 aa overlap and to SW:BCRA_BACLI (EMBL:L20573) Bacillus licheniformis bacitracin transport ATP-binding protein BcrA, 306 aa; fasta scores: opt: 581 z-score: 562.3 E(): 6.6e-24; 38.8% identity in 304 aa overlap. Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and matches to Prosite entries PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Contains also possible N-terminal region signal peptide sequence 4 2 7 11 [Reporter]SCO4627 (11P19)_PCR/3670 [Gene]SCO4627/6675 NC_003888 SCD39.27c, unknown, len: 89 aa 4 2 7 10 [Reporter]SCO2928 (12L19)_PCR/3669 [Gene]SCO2928/6673 NC_003888 SCE19A.28, probable asnC-family transcriptional regulator, len: 163 aa; similar to SW:ASNC_ECOLI (EMBL:K00826), AsnC, Escherichia coli transcriptional activator of asparagine synthetase (152 aa), fasta scores; opt: 236 z-score: 296.2 E(): 3.7e-09, 31.3% identity in 147 aa overlap and to putative asnC-family regulators. Similar to SC6G9.10 (EMBL:AL079356) S.coelicolor probable AsnC family transcriptional regulator (164 aa) (48.7% identity in 156 aa overlap). Contains Pfam match to entry PF01037 ASNC_trans_reg, AsnC family 4 2 7 9 [Reporter]SCO0720 (13H19)_PCR/3668 [Gene]SCO0720/6671 NC_003888 SCF42.30c, possible integral membrane protein, len: 497 aa. Weakly similar to several proteins of unknown function e.g. Mycobacterium tuberculosis SW:YT19_MYCTU(EMBL:Z79700) (455 aa), fasta scores opt: 157 z-score: 177.3 E(): 0.017 27.1% identity in 450 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 2 7 8 [Reporter]SCO1820 (14D19)_PCR/3667 [Gene]SCO1820/6669 NC_003888 SCI8.05, possible integral membrane protein, len: 159 aa. Contains possible membrane spanning hydrophobic domains. 4 2 7 7 [Reporter]SCO0227 (15P15)_PCR/3666 [Gene]SCO0227/6667 NC_003888 SCJ9A.06c, unknown, len: 120aa; predicted by GC Frameplot and amino acid usage. 4 2 7 6 [Reporter]SCO0933 (16L15)_PCR/3665 [Gene]SCO0933/6665 NC_003888 SCM10.21, possible lipoprotein, len: 172 aa. Contains correctly situated match to Prosite entry PS00013 Prokaryotic membrane lipoprotein lipid attachment site and possible N-terminal region signal peptide sequence 4 2 7 5 [Reporter]SCO3412 (17H15)_PCR/3664 [Gene]SCO3412/6663 NC_003888 SCE9.19, conserved hypothetical protein, len: 106 aa; unknown function, weakly similar to e.g. TR:O26644 (EMBL:AE000837) Methanobacterium thermoautotrophicum hypothetical protein (127 aa), fasta scores; opt: 338 z-score: 369.7 E(): 2.9e-13, 50.5% identity in 107 aa overlap 4 2 7 4 [Reporter]SCO2937 (18D15)_PCR/3663 [Gene]SCO2937/6661 NC_003888 SCE56.01c, possible transport protein (partial), len: >170 aa; similar to TR:AAF11647 (EMBL:AE002045) Deinococcus radiodurans conserved hypothetical protein DR2098, 462 aa; fasta scores: opt: 330 z-score: 380.0 E(): 9.3e-14; 37.4% identity in 155 aa overlap and to TR:O69070 (EMBL:AF079900) Streptomyces rimosus tetracycline efflux protein OtrB, 563 aa; fasta scores: opt: 271 z-score: 312.6 E(): 5.3e-10; 34.8% identity in 158 aa overlap. Contains possible hydrophobic membrane spanning regions,SCE19A.37c, possible transmembrane transport protein, partial CDS, len: >344 aa; weak similarity to transport proteins, many hypothetical e.g. TR:Q50392 (EMBL:U40487), lfrA, Mycobacterium smegmatis proton antiporter efflux pump (504 aa), fasta scores; opt: 220 z-score: 251.9 E(): 1.1e-06, 27.8% identity in 345 aa overlap. Weak similarity to part of others from S.coelicolor e.g. TR:Q9ZBJ1 (EMBL:AL035161) S.coelicolor probable efflux protein (815 aa) (27.2% identity in 345 aa overlap). Contains hydrophobic, possible membrane-spanning regions 4 2 7 3 [Reporter]SCP1.05c (19P11)_PCR/3662 [Gene]SCP1.05c/6659 NC_003888 SCP1.05c, unknown, len: 131aa; 4 2 7 2 [Reporter]SCO1855 (20H11)_PCR/3661 [Gene]SCO1855/6657 NC_003888 SCI39.02, probable precorrin-4 C11-methyltransferase, len: 281 aa; similar to TR:O87696 (EMBL:AJ000758) Bacillus megaterium precorrin-4 methylase CbiF, 258 aa; fasta scores: opt: 674 Z-score: 771.2 bits: 150.4 E(): 2.3e-35; 41.502% identity in 253 aa overlap and to SW:COBM_PSEDE (EMBL:M59301) Pseudomonas denitrificans precorrin-4 C11-methyltransferase (EC 2.1.1.133) CobM, 253 aa; fasta scores: opt: 652 Z-score: 746.5 bits: 145.8 E(): 5.5e-34; 43.145% identity in 248 aa overlap . Contains Pfam match to entry PF00590 TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases 4 2 6 22 [Reporter]SCO5246 (1H7)_PCR/3659 [Gene]SCO5246/6655 NC_003888 2SC7G11.08, possible Na+/H+ antiporter, len: 530 aa; identical to C-terminal region of previously sequenced TR:Q9RPC9 (EMBL:AF162936) Streptomyces coelicolor A3(2) Na/H antiporter homolog (fragment), 212 aa and similar to SW:YJCE_ECOLI: (EMBL:U00006) Escherichia coli putative Na(+)/H(+) exchanger YjcE, 549 aa; fasta scores: opt: 516 z-score: 557.0 E(): 1.5e-23; 30.1% identity in 552 aa overlap. Contains Pfam match to entry PF00999 Na_H_Exchanger, Sodium/hydrogen exchanger family and possible hydrophobic membrane spanning regions 4 2 6 21 [Reporter]SCO2779 (2D7)_PCR/3658 [Gene]SCO2779/6653 NC_003888 SCC105.10, acdH, acyl-CoA dehydrogenase, len: 386 aa; identical to previously sequenced TR:Q9XCG6 (EMBL:AF142581) Streptomyces coelicolor acyl-CoA dehydrogenase AcdH, 386 aa. Contains Pfam match to entry PF00441 Acyl-CoA_dh, Acyl-CoA dehydrogenase and matches to Prosite entries PS00072 Acyl-CoA dehydrogenases signature 1 and PS00073 Acyl-CoA dehydrogenases signature 2 4 2 6 20 [Reporter]SCO4712 (3P3)_PCR/3657 [Gene]SCO4712/6651 NC_003888 SCD31.37, rplN, 50S ribosomal protein L14, len: 122 aa; highly similar to SW:RL14_BACST (PIR:A02789) Bacillus stearothermophilus 50S ribosomal protein L14 RplN, 122 aa; fasta scores: opt: 630 z-score: 783.7 E(): 0; 77.9% identity in 122 aa overlap. Contains Pfam match to entry PF00238 Ribosomal_L14, Ribosomal protein L14 and match to Prosite entry PS00049 Ribosomal protein L14 signature 3 1 12 7 [Reporter]SCO0477 (15C24)_PCR/1256 [Gene]SCO0477/2299 NC_003888 SCF76.17, conserved hypothetical protein, len: 130 aa. Highly similar to several including: Bacillus subtilis TR:O32161 (EMBL; Z99120) YurT protein (127 aa), fasta scores opt: 494 z-score: 605.8 E(): 2.2e-26 58.6% identity in 128 aa overlap, Streptomyces coelicolor TR:CAB42950 (EMBL; AL049863) hypothetical 14.4 KD protein SC5H1.25C (131 aa), fasta scores opt: 221 z-score: 280.0 E(): 3.1e-08 37.2% identity in 129 aa overlap and also weakly similar to some lyases e.g. Salmonella typhimurium SW:LGUL_SALTY (EMBL; U57364) lactoylglutathione lyase (EC 4.4.1.5) (135 aa), fasta scores opt: 101 z-score: 136.7 E(): 2.9 23.1% identity in 134 aa overlap 4 2 6 19 [Reporter]SCO6504 (4L3)_PCR/3656 [Gene]SCO6504/6649 NC_003888 SC1E6.13, unknown, len: 376 aa; some similarity in part to YMEL_STRLN Streptomyces lincolnensis hypothetical protein (150 aa), fasta scores; opt: 377 z-score: 448.8 E(): 1e-17, 36.1% identity in 144 aa overlap. Contains highly acidic repetitive region from aa 290-376 4 2 6 18 [Reporter]SCO6352 (5H3)_PCR/3655 [Gene]SCO6352/6647 NC_003888 SC3A7.20c, unknown, len: 138 aa 4 2 6 17 [Reporter]SCO6637 (6D3)_PCR/3654 [Gene]SCO6637/6645 NC_003888 SC4G2.11c, unknown, len: 350 aa; identical to TR:Q53944 (EMBL:L37531) ORF3 downstream of PglZ (350 aa) 4 2 6 16 [Reporter]SCO4849 (6P23)_PCR/3653 [Gene]SCO4849/6643 NC_003888 SC5G8.17, possible integral membrane protein, len: 436 aa; N-terminal region contains possible membrane-spanning hydrophobic regions while the remainder is similar to many hypothetical proteins e.g. TR:Q9ZM43 (EMBL:AE001473) putative from Helicobacter pylori J99 (370 aa) fasta scores; opt: 596, z-score: 590.7, E(): 1.9e-25, 37.4% identity in 273 aa overlap 4 2 6 15 [Reporter]SCO6972 (7L23)_PCR/3652 [Gene]SCO6972/6641 NC_003888 SC6F7.25c, unknown, len: 179 aa. 3 1 12 2 [Reporter]SCO3980 (20K16)_PCR/1251 [Gene]SCO3980/2289 NC_003888 SCBAC25E3.17c, unknown, len: 191 aa: no significant database matches. 4 2 6 13 [Reporter]SCO6066 (9D23)_PCR/3650 [Gene]SCO6066/6639 NC_003888 SC9B1.13c, cvnD6, possible ATP/GTP-binding protein, len: 181aa; similar to others egs. TR:O50391 (EMBL:AL009198) hypothetical protein from Mycobacterium tuberculosis (193 aa) fasta scores; opt: 689, z-score: 849.1, E(): 0, (61.4% identity in 166 aa overlap) and two putative ATP/GTP-binding proteins from Streptomyces coelicolor: TR:O86519 (EMBL:AL031124) (174 aa) fasta scores; opt: 665, z-score: 820.6, E(): 0, (55.0% identity in 171 aa overlap) and TR:CAA19989 (EMBL:AL031124) (174 aa) fasta scores; opt: 662, z-score: 817.0, E(): 0, (55.6% identity in 171 aa overlap). Contains Prosite match to PS00017 ATP/GTP-binding site motif A (P-loop). 4 2 6 12 [Reporter]SCO2741 (10P19)_PCR/3649 [Gene]SCO2741/6637 NC_003888 SCC57A.12, possible secreted protein, len: 169 aa. Contains a possible N-terminal signal sequence. 4 2 6 11 [Reporter]SCO4622 (11L19)_PCR/3648 [Gene]SCO4622/6635 NC_003888 SCD39.22c, possible integral membrane protein, len: 166 aa. Contains possible hydrophobic membrane spanning regions 4 2 6 10 [Reporter]SCO4301 (12H19)_PCR/3647 [Gene]SCO4301/6633 NC_003888 SCD95A.34c, possible DNA-binding protein, len: 279 aa; similar to various S. coelicolor hypothetical proteins, e.g. TR:CAB69734 (EMBL:AL137166) Streptomyces coelicolor hypothetical 30.4 kD protein SCC121.16, 277 aa; fasta scores: opt: 731 z-score: 897.3 E(): 0; 45.2% identity in 259 aa overlap and to TR:CAB88448 (EMBL:AL353815) Streptomyces coelicolor putative DNA-binding protein SCD6.19, 295 aa; fasta scores: opt: 388 z-score: 478.8 E(): 3.3e-19; 34.3% identity in 286 aa overlap. Contains TTA leucine codon, possible target for bldA regulation and a possible helix-turn-helix motif at residues 30..51 (+3.39 SD) 4 2 6 9 [Reporter]SCO3379 (13D19)_PCR/3646 [Gene]SCO3379/6631 NC_003888 SCE94.30c, unknown, proline-rich, len: 208aa; 3 1 11 17 [Reporter]SCO2062 (6G8)_PCR/1244 [Gene]SCO2062/2279 NC_003888 SC4G6.31c, unknown, len: 403aa; 4 2 6 8 [Reporter]SCO0816 (14P15)_PCR/3645 [Gene]SCO0816/6629 NC_003888 SCF43A.06, conserved possible iron-sulfur protein, len: 492 aa; similar to several putative iron-sulfur proteins e.g. SW:YKGF_ECOLI (EMBL:AE000137) Escherichia coli hypothetical protein (475 aa), fasta scores; opt: 1148 z-score: 1249.5 E(): 0, 39.7% identity in 481 aa overlap, which contains 2 putative 4Fe-4S centres. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature 4 2 6 7 [Reporter]SCO2938 (15L15)_PCR/3644 [Gene]SCO2938/6627 NC_003888 SCE56.02, possible oxidoreductase, len: 336 aa; similar to TR:CAB61562 (EMBL:AL133171) Streptomyces coelicolor putative aldo/keto reductase (fragment) SCF81.28c, 307 aa; fasta scores: opt: 1370 z-score: 1572.6 E(): 0; 65.2% identity in 305 aa overlap and to SW:NORA_ASPFL (EMBL:U32377) Aspergillus flavus norsolorinic acid reductase (EC 1.1.1.-) NorA, 388 aa; fasta scores: opt: 572 z-score: 659.1 E(): 2.7e-29; 32.8% identity in 329 aa overlap 4 2 6 6 [Reporter]SCO3229 (16H15)_PCR/3643 [Gene]SCO3229/6625 NC_003888 SCE63.04, probable 4-hydroxyphenylpyruvic acid dioxygenase, len: 371 aa; similar to many both prokaryotic and eukaryotic e.g. TR:O52791 (EMBL:AJ223998) similar to hydroxyphenyl pyruvate dioxygenase from a cluster of genes involved in the biosynthesis of a vancomycin group antibiotic in Amycolatopsis orientalis (Actinomycete) (357 aa) fasta scores; opt: 989, z-score: 1130.3, E(): 0, (48.7% identity in 355 aa overlap) and SW:HPPD_MOUSE hpd, 4-hydroxyphenylpyruvic acid dioxygenase from Mus musculus (mouse) (392 aa) fasta scores; opt: 610, z-score: 698.5, E(): 1.3e-31, (31.6% identity in 361 aa overlap). 4 2 6 5 [Reporter]SCO0443 (17D15)_PCR/3642 [Gene]SCO0443/6623 NC_003888 SCF51A.21, unknown, len: 285 aa. Similar to a number of hypothetical proteins e.g. Mycobacterium tuberculosis TR:P95220 (EMBL; Z86089) hypothetical 32.2 KD protein (300 aa), fasta scores opt: 714 z-score: 663.0 E(): 1.4e-29 45.3% identity in 289 aa overlap. Also similar in parts to Pseudomonas fluorescens TR:CAB48407 (EMBL; AJ243652) urea amidolyase homologue (1213 aa), fasta scores opt: 423 z-score: 390.1 E(): 2.3e-14 37.7% identity in 289 aa overlap 4 2 6 4 [Reporter]SCO0889 (18P11)_PCR/3641 [Gene]SCO0889/6621 NC_003888 SCM1.22c, possible LysR-family transcriptional regulatory protein, len: 301 aa. Similar to many other regulatory proteins including: Escherichia coli SW:OXYR_ECOLI (EMBL:J04553) hydrogen peroxide-inducible genes activator (305 aa), fasta scores opt: 348 z-score: 401.9 E(): 4.8e-15 28.4% identity in 299 aa overlap and Streptomyces coelicolor TR:O86805 (EMBL:AL031317) putative transcriptional regulator (306 aa), fasta scores opt: 491 z-score: 563.9 E(): 4.6e-24 36.4% identity in 302 aa overlap. Contains a Pfam match to entry PF00126 HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family, with the putative helix-turn-helix motif situated between residues 17..38 (+3.88 SD). 3 1 11 12 [Reporter]SCO2269 (11C4)_PCR/1239 [Gene]SCO2269/2269 NC_003888 SCC75A.15c, unknown, len: 275 aa. 4 2 6 3 [Reporter]SCO4053 (19L11)_PCR/3640 [Gene]SCO4053/6619 NC_003888 2SCD60.19, possible transport integral membrane protein, len: 589 aa; similar to TR:P94432 (EMBL:D50453) Bacillus subtilis homologue of copper export protein YcnJ, 541 aa; fasta scores: opt: 394 z-score: 369.0 E(): 5.4e-13; 27.8% identity in 442 aa overlap. Contains possible hydrophobic membrane spanning regions 4 2 6 2 [Reporter]SCO6093 (20D11)_PCR/3639 [Gene]SCO6093/6617 NC_003888 SCBAC1A6.17c, possible secreted protein, len: 248 aa; similar to TR:Q9L2E8 (EMBL:AL137187) Streptomyces coelicolor putative secreted protein SC7A8.10c, 274 aa; fasta scores: opt: 552 z-score: 639.4 E(): 4.9e-28; 45.7% identity in 234 aa overlap. Contains possible cleavable N-terminal region signal peptide sequence 4 2 5 22 [Reporter]SCO5396 (1D7)_PCR/3637 [Gene]SCO5396/6615 NC_003888 2SC6G5.40c, probable cellulose-binding protein, len: 310aa; strongly similar to TR: (EMBL:) AbpS avicel-binding protein from Streptomyces reticuli (311 aa) fasta scores; opt: 1777, z-score: 1598.0, E(): 0, 93.2% identity in 311 aa overlap. Contains a possible membrane-spanning hydrophobic region close to the N-terminus and possible coiled-coil regions throughout. 4 2 5 21 [Reporter]SCO5961 (2P3)_PCR/3636 [Gene]SCO5961/6613 NC_003888 SC7H1.31c, cbiM, probably involved in cobalt transport, len: 257 aa; similar to many eg. CBIM_SALTY Q05594 cbim protein. salmonella typhimurium. (245 aa), fasta scores; opt: 749 z-score: 1155.1 E(): 0, 53.6% identity in 224 aa overlap. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature 4 2 5 20 [Reporter]SCO4652 (3L3)_PCR/3635 [Gene]SCO4652/6611 NC_003888 SCD82.23, rplJ, 50S ribosomal protein L10, len: 176 aa; identical to previously sequenced SW:RL10_STRCO (EMBL:L24552) Streptomyces coelicolor 50S ribosomal protein L10 RplJ. Contains Pfam match to entry PF00466 Ribosomal_L10, Ribosomal protein L10 and match to Prosite entry PS01109 Ribosomal protein L10 signature 3 1 11 7 [Reporter]SCO0602 (15O20)_PCR/1234 [Gene]SCO0602/2259 NC_003888 SCF55.26, hypothetical protein, len: aa; similar to various hypothetical proteins, e.g. TR:CAB42732 (EMBL:AL049826) Streptomyces coelicolor hypothetical 42.9 KD protein SCH24.26c, 373 aa; fasta scores: opt: 1649 z-score: 1932.2 E(): 0; 61.8% identity in 374 aa overlap and low similarity to TR:O51533 (EMBL:AE001160) Borrelia burgdoferi FemA protein (FemA) BB0586, 347 aa; fasta scores: opt: 307 z-score: 363.4 E(): 7.2e-13; 27.4% identity in 197 aa overlap 4 2 5 19 [Reporter]SCO6907 (4H3)_PCR/3634 [Gene]SCO6907/6609 NC_003888 SC1B2.13, possible DNA ligase, len: 257 aa. Similar in parts to Archaeoglobus fulgidus SW:DNLI_ARCFU(EMBL:AE001061) DNA ligase (EC 6.5.1.1) (556 aa), fasta scores opt: 175 z-score: 199.4 E(): 0.0012 28.9% identity in 173 aa overlap and to Streptomyces coelicolor TR:Q9XAM3(EMBL:AL079355) putative DNA ligase, SC4C6.17C (355 aa), fasta scores opt: 366 z-score: 413.9 E(): 1.4e-15 32.9% identity in 237 aa overlap. 4 2 5 18 [Reporter]SCO6348 (5D3)_PCR/3633 [Gene]SCO6348/6607 NC_003888 SC3A7.16c, putative membrane protein, len: 1361 aa. Contains possible hydrophobic membrane spanning regions 4 2 5 17 [Reporter]SCO7072 (5P23)_PCR/3632 [Gene]SCO7072/6605 NC_003888 SC4G1.38c, hypothetical protein, len: 199 aa; similar to SW:YCGS_ECOLI (EMBL:AE000218) Escherichia coli hypothetical 22.6 kDa protein in TreA-Pth intergenic region YcgS, 210 aa; fasta scores: opt: 349 z-score: 387.3 E(): 4.2e-14; 44.5% identity in 200 aa overlap and to C-terminal region of SW:DAK1_YEAST (EMBL:Z38114) Saccharomyces cerevisiae dihydroxyacetone kinase 1 (EC 2.7.1.29) Dak1, 584 aa; fasta scores: opt: 298 z-score: 326.4 E(): 1e-10; 33.3% identity in 189 aa overlap 4 2 5 16 [Reporter]SCO4845 (6L23)_PCR/3631 [Gene]SCO4845/6603 NC_003888 SC5G8.13, possible integral membrane protein, len: 157aa; similar to many eg. SW:P72055 (Y1I9_MYCTU) hypothetical protein from Mycobacterium tuberculosis (121 aa) fasta scores; opt: 139, z-score: 176.2, E(): 0.023, 31.7% identity in 126 aa overlap. Contains possible membrane-spanning hydrophobic regions. 4 2 5 15 [Reporter]SCO1437 (7H23)_PCR/3630 [Gene]SCO1437/6601 NC_003888 SC6D7.02, possible membrane protein, len: 156 aa. Weakly similar to a hypothetical protein from Mycobacterium tuberculosis: SW:YE17_MYCTU(EMBL:Z80108) (154 aa), fasta scores opt: 212 z-score: 266.9 E(): 1.7e-07 32.4% identity in 136 aa overlap. Contains possible membrane spanning hydrophobic domains. 3 1 11 2 [Reporter]SCO3982 (20G16)_PCR/1229 [Gene]SCO3982/2249 NC_003888 SCBAC25E3.19, conserved hypothetical protein, len: 92 aa: strongly similar to TR:Q9X8E1 (EMBL:AL049573) hypothetical protein SCE39.18c from Streptomyces coelicolor (92 aa) fasta scores; opt: 471, Z-score: 618.1, 81.522% identity in 92 aa overlap. Contains TTA leucine codon, possible target for bldA regulation 4 2 5 14 [Reporter]SCO5942 (8D23)_PCR/3629 [Gene]SCO5942/6599 NC_003888 SC7H1.12, unknown, len: 205 aa; very weak similarity to downstream ORF SC7H1.21 (203 aa), (E(): 0.085, 26.9% identity in 208 aa overlap). Contains possible Helix-turn-helix motif at aa 41-62, Score 1089 (+2.90 SD) 4 2 5 13 [Reporter]SCO6451 (9P19)_PCR/3628 [Gene]SCO6451/6597 NC_003888 SC9B5.18, probable substrate binding protein, len: 467aa; First of a five gene operon probably involved in peptide uptake. Very similar to SW:XP55_STRLI protein precursor of XP55, a major secreted protein from Streptomyces lividans (542 aa) fasta scores: opt: 3353, z-score: 2613.8, E(): 0, (95.9% identity in 532 aa overlap). Also similar to many substrate binding /transport proteins eg. SW:APPA_BACSU AppA oligopeptide-binding protein precursor from Bacillus subtilis (543 aa) fasta scores: opt: 717, z-score: 518.2, E(): 1.4e-21, (28.7% identity in 550 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site and PS01040 Bacterial extracellular solute-binding proteins, family 5 signature. Also contains Pfam match to entry PF00496 SBP_bac_5, Bacterial extracellular solute-binding proteins, family 5. 4 2 5 12 [Reporter]SCO2733 (10L19)_PCR/3627 [Gene]SCO2733/6595 NC_003888 SCC57A.04c, unknown, len: 279 aa. Similar to several proteins of unconfirmed function including: Streptomyces coelicolor TR:CAB58282(EMBL:AL121850) conserved hypothetical protein SCC78.01C (fragment), fasta scores opt: 274 z-score: 319.9 E(): 2e-10 35.3% identity in 235 aa overlap and Streptomyces nogalater TR:AAF01810(EMBL:AF187532) putative hydroxylase (275 aa), fasta scores opt: 384 z-score: 443.4 E(): 2.6e-17 34.9% identity in 281 aa overlap. 4 2 5 11 [Reporter]SCO4591 (11H19)_PCR/3626 [Gene]SCO4591/6593 NC_003888 SCD20.09, hypothetical protein, len: 190 aa; similar to TR:Q9RJT3 (EMBL:AL132707) Streptomyces coelicolor hypothetical 21.7 kDa protein SCF51.02c, 205 aa; fasta scores: opt: 325 z-score: 395.2 E(): 1.5e-14; 40.4% identity in 166 aa overlap 4 2 5 10 [Reporter]SCO4297 (12D19)_PCR/3625 [Gene]SCO4297/6591 NC_003888 SCD95A.30, probable oxidoreduxtase, len: 376 aa; similar to SW:NADO_THEBR (EMBL:X67220) Thermoanaerobacter brockii NADH oxidase, 651 aa; fasta scores: opt: 498 z-score: 552.0 E(): 2.8e-23; 33.6% identity in 354 aa overlap. Contains Pfam match to entry PF00724 oxidored_FMN, FMN oxidoreductase 4 2 5 9 [Reporter]SCO0233 (13P15)_PCR/3624 [Gene]SCO0233/6589 NC_003888 SCJ9A.12c, possible DNA-binding protein, len: 279 aa; N-terminal similar to TR:O86721 (EMBL:AL031515) hypothetical protein from Streptomyces coelicolor (272 aa) fasta scores; opt: 135, z-score: 165.1, E(): 0.079, (29.3% identity in 150 aa overlap). Contains possible helix-turn-helix motif (+3.31 SD) 37-58 aa 4 2 5 8 [Reporter]SCO0279 (14L15)_PCR/3623 [Gene]SCO0279/6587 NC_003888 SCF85.07, possible glycosyl hydrolase, len; 423 aa. Shares a low level of similarity with many alpha-l-fucosidases e.g. Thermotoga maritima TR:AAD35394 (EMBL:AE001712) alpha-l-fucosidase, putative (449 aa), fasta scores opt: 332 z-score: 363.4 E(): 6.9e-13 35.8% identity in 358 aa overlap. 4 2 5 7 [Reporter]SCO0213 (15H15)_PCR/3622 [Gene]SCO0213/6585 NC_003888 SCJ12.25c, possible nitrate/nitrite transporter protein, len: 412 aa. Similar to Bacillus subtilis SW:NARK_BACSU (EMBL; Z49884) nitrite extrusion protein (395 aa), fasta scores opt: 834 z-score: 877.9 E():0 36.8% identity in 378 aa overlap. Contains multiple possible membrane spanning hydrophobic domains. 4 2 5 6 [Reporter]SCO3367 (16D15)_PCR/3621 [Gene]SCO3367/6583 NC_003888 SCE94.18, possible tetR-family regulator, len: 195 aa; similar to many regulators e.g. TR:G4240425 (EMBL:AF080235) LanK from Streptomyces cyanogenus S136 (192 aa) fasta scores; opt: 584, z-score: 737.3, E(): 0, (51.4% identity in 185 aa overlap). Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and Prosite match to PS01081 Bacterial regulatory proteins, tetR family signature. Also contains a probable helix-turn-helix at 43-55aa (+5.78 SD). 4 2 5 5 [Reporter]SCO1061 (17P11)_PCR/3620 [Gene]SCO1061/6581 NC_003888 SCG22.07, probable bifunctional protein (secreted sugar binding protein/sugar hydrolase), len: 555 aa; N-terminal domain similar to N-terminal domain of TR:O86727 (EMBL:AL031515) Streptomyces coelicolor putative secreted cellulase SC5C7.30c, 890 aa; blastp scores: Score = 84 (29.6 bits), Expect = 0.031, Sum P(3) = 0.030 Identities = 61/233 (26%), Positives = 89/233 (38%) and C-terminal domain similar to C-terminal domain of SW:E13B_OERXA (EMBL:AF052745) Oerskovia xanthineolytica glucan enco-1,3-glucosidase precursor (EC 3.2.1.39), 548 aa; fasta scores: opt: 368 Z-score: 394.8 E(): 2.3e-14; 41.447% identity) in 152 aa overlap. Contains 3x Pfam match to entry PF02012 BNR, BNR repeat and Pfam match to entry PF00652 Ricin_B_lectin, Similarity to lectin domain of ricin beta-chain, 3 copies. Contains also possible N-terminal region signal peptide sequence 4 2 5 4 [Reporter]SCO0592 (18L11)_PCR/3619 [Gene]SCO0592/6579 NC_003888 SCF55.16c, unknown, len: 240 aa 4 2 5 3 [Reporter]SCO4882 (19H11)_PCR/3618 [Gene]SCO4882/6577 NC_003888 2SCK8.08, unknown, len: 355 aa 4 2 5 2 [Reporter]SCO1719 (20P7)_PCR/3617 [Gene]SCO1719/6575 NC_003888 SCI11.08, possible ABC-transporter ATP-binding protein, len: 332 aa; similar to members of the ABC-transporter superfamily e.g. SW:DRRA_STRPE (EMBL:M73758), drrA, Streptomyces peucetius daunorubicin resistance ATP-binding protein (330 aa), fasta scores; opt: 901 z-score: 964.0 E(): 0, 50.6% identity in 316 aa overlap. Similar to many from S.coelicolor e.g. SCE9.25c (EMBL:AL049841) S.coelicolor probable ABC transporter ATP-binding component (315 aa) (57.1% identity in 308 aa overlap). Contains Pfam match to entry PF00005 ABC_tran, ABC transporter and PS00017 ATP/GTP-binding site motif A (P-loop) 4 2 4 22 [Reporter]SCO4194 (1P3)_PCR/3615 [Gene]SCO4194/6573 NC_003888 2SCD46.08, possible TetR-family transcriptional regulator, len: 213 aa; similar to many others from streptomycetes e.g. TR:CAB88457 (EMBL:AL353815) putative TetR-family transcriptional regulator from Streptomyces coelicolor (215 aa) fasta scores; opt: 369, z-score: 450.8, E(): 1.3e-17, 37.3% identity in 212 aa overlap and SW:P39885 (TCMR_STRGA) tetracenomycin c transcriptional repressor from Streptomyces glaucescens (226 aa) fasta scores; opt: 246, z-score: 303.8, E(): 2e-09, 36.7% identity in 166 aa overlap. Contains Pfam match to entry PF00440 tetR, Bacterial regulatory proteins, tetR family and helix-turn-helix motif (Score 1437 (+4.08 SD)) at residues 40..61 4 2 4 21 [Reporter]SCO5723 (2L3)_PCR/3614 [Gene]SCO5723/6571 NC_003888 SC3C3.09c, possible regulator, BldB, len: 98 aa; identical to TR:Q53842 (EMBL:U28930) bldB (98 aa) and similar to S. coelicolor TR:Q53896 (EMBL:X60316) AbaA ORFD (75 aa), fasta scores; opt: 150 z-score: 201.9 E(): 0.00055, 47.4% identity in 57 aa overlap 4 2 4 20 [Reporter]SCO5443 (3H3)_PCR/3613 [Gene]SCO5443/6569 NC_003888 SC6A11.19c, pep1A, possible alpha-amylase, len: 675 aa; previously sequenced as TR:O54202 (EMBL:AJ001205). Also similar to TRNEW:AAF07898 (EMBL:AF172946) putative glucanase GlgE from Mycobacterium smegmatis (697 aa) fasta scores; opt: 2199, z-score: 2484.5, E(): 0, 53.6% identity in 679 aa overlap. Contains Pfam match to entry PF00128 alpha-amylase, Alpha amylase 4 2 4 19 [Reporter]SCO6903 (4D3)_PCR/3612 [Gene]SCO6903/6567 NC_003888 SC1B2.09, unknown, len: 266 aa. Weakly similar to Streptomyces coelicolor TR:CAB72355(EMBL:AL138977) hypothetical 27.8 kd protein, SC7F9.02C (259 aa), fasta scores opt: 149 z-score: 163.3 E(): 0.12 30.0% identity in 283 aa overlap. 4 2 4 18 [Reporter]SCO7081 (4P23)_PCR/3611 [Gene]SCO7081/6565 NC_003888 SC3A4.07c, possible phospholipase, len: 395 aa. Similar to several including: Bacillus firmus TR:O66043(EMBL:U88888) cardiolipin synthase, ClS (503 aa), fasta scores opt: 605 z-score: 748.2 E():0 31.8% identity in 377 aa overlap. Contains 2xPfam matches to entry PF00614 PLDc, Phospholipase D. Active site motif. 4 2 4 17 [Reporter]SCO7068 (5L23)_PCR/3610 [Gene]SCO7068/6563 NC_003888 SC4G1.34c, possible secreted protein, len: 183 aa; similar to TR:CAB69767 (EMBL:AL137187) Streptomyces coelicolor putative secreted protein SC7A8.18, 180 aa; fasta scores: opt: 171 z-score: 179.1 E(): 0.017; 32.8% identity in 192 aa overlap. Contains possible N-terminal region signal peptide sequence 1 1 10 1 [Reporter]23s rRNA 1:4 (CtrlI12)_PCR/198 control_biosequence 1 1 9 21 [Reporter]no spot (2M8)_PCR/196 1 1 9 8 [Reporter]no spot (14I20)_PCR/183 1 3 4 1 [Reporter]16s rRNA 1:4 (CtrlA6)_PCR/4098 control_biosequence 1 1 9 1 [Reporter]23s rRNA 1:1 (CtrlM12)_PCR/176 control_biosequence 1 3 3 2 [Reporter]no spot (20E6)_PCR/4077 1 3 3 1 [Reporter]16s rRNA 1:1 (CtrlE6)_PCR/4076 control_biosequence 1 3 2 20 [Reporter]no spot (2M22)_PCR/4073 1 1 8 5 [Reporter]no spot (17I16)_PCR/158 1 1 8 1 [Reporter]23s carry-over (CtrlA16)_PCR/154 1 1 7 20 [Reporter]no spot (3A8)_PCR/151 1 1 7 19 [Reporter]no spot (4M4)_PCR/150 1 1 7 11 [Reporter]no spot (11M20)_PCR/142 1 3 2 1 [Reporter]16s carry-over (CtrlI6)_PCR/4054 1 1 7 1 [Reporter]16s rRNA 1:256 (CtrlE4)_PCR/132 control_biosequence 1 1 6 14 [Reporter]no spot (8E24)_PCR/123 1 3 1 1 [Reporter]Cy3 lights (CtrlE22)_PCR/4033 control_unknown_type 1 1 6 1 [Reporter]16s rRNA 1:64 (CtrlI4)_PCR/110 control_biosequence 1 1 5 22 [Reporter]no spot (1A8)_PCR/109 4 2 23 1 [Reporter]Cy5 lights (CtrlP23)_PCR/4012 control_unknown_type 4 4 21 1 [Reporter]P10 (CtrlD21)_PCR/7999 4 4 20 21 [Reporter]no spot (2L17)_PCR/7997 4 4 20 17 [Reporter]no spot (6L13)_PCR/7993 4 4 20 1 [Reporter]P8 (CtrlP17)_PCR/7977 4 4 19 20 [Reporter]no spot (3D17)_PCR/7974 4 4 19 14 [Reporter]no spot (9L9)_PCR/7968 4 4 19 1 [Reporter]P7 (CtrlL17)_PCR/7955 4 2 4 4 [Reporter]no spot (18H11)_PCR/3597 4 2 4 2 [Reporter]no spot (20L7)_PCR/3595 4 2 4 1 [Reporter]16s rRNA 1:4 (CtrlD7)_PCR/3594 control_biosequence 4 2 3 20 [Reporter]no spot (3D3)_PCR/3591 4 4 18 15 [Reporter]no spot (8L9)_PCR/7947 4 2 3 15 [Reporter]no spot (7P19)_PCR/3586 4 4 18 6 [Reporter]no spot (17H1)_PCR/7938 4 4 18 1 [Reporter]P6 (CtrlH17)_PCR/7933 4 2 3 5 [Reporter]no spot (17H11)_PCR/3576 4 2 3 4 [Reporter]no spot (18D11)_PCR/3575 4 2 3 1 [Reporter]16s rRNA 1:1 (CtrlH7)_PCR/3572 control_biosequence 4 2 2 22 [Reporter]no spot (1H3)_PCR/3571 4 4 17 1 [Reporter]P5 (CtrlD17)_PCR/7911 4 2 2 1 [Reporter]16s carry-over (CtrlL7)_PCR/3550 4 4 16 15 [Reporter]no spot (8D9)_PCR/7903 4 2 1 10 [Reporter]no spot (12D15)_PCR/3537 4 2 1 1 [Reporter]Cy3 lights (CtrlH23)_PCR/3529 control_unknown_type 3 2 23 5 [Reporter]no spot (18G7)_PCR/3511 3 2 23 1 [Reporter]Cy5 lights (CtrlO23)_PCR/3508 control_unknown_type 4 4 16 5 [Reporter]no spot (17L21)_PCR/7893 4 4 16 1 [Reporter]P4 (CtrlP13)_PCR/7889 4 4 15 16 [Reporter]no spot (7D9)_PCR/7882 4 4 15 1 [Reporter]P3 (CtrlL13)_PCR/7867 4 4 14 15 [Reporter]no spot (8L5)_PCR/7859 3 2 22 9 [Reporter]no spot (14C11)_PCR/3494 3 2 22 7 [Reporter]no spot (16K7)_PCR/3492 4 4 14 1 [Reporter]P1 (CtrlH13)_PCR/7845 3 2 22 2 [Reporter]50% DMSO (CtrlC3)_PCR/3487 3 2 22 1 [Reporter]Cy3 lights carry-over (CtrlK23)_PCR/3486 control_unknown_type 3 2 21 10 [Reporter]no spot (13C11)_PCR/3473 4 4 13 1 [Reporter]23s rRNA 1:256 (CtrlP5)_PCR/7823 control_biosequence 3 2 21 1 [Reporter]P10 (CtrlC23)_PCR/3464 4 4 12 15 [Reporter]no spot (8D5)_PCR/7815 4 4 12 12 [Reporter]no spot (11H1)_PCR/7812 4 4 12 4 [Reporter]no spot (18H17)_PCR/7804 4 4 12 1 [Reporter]23s rRNA 1:64 (CtrlD9)_PCR/7801 control_biosequence 3 2 20 1 [Reporter]P8 (CtrlO19)_PCR/3442 3 2 19 14 [Reporter]no spot (9K11)_PCR/3433 3 2 19 3 [Reporter]no spot (19O23)_PCR/3422 3 2 19 1 [Reporter]P7 (CtrlK19)_PCR/3420 3 2 18 5 [Reporter]no spot (18C3)_PCR/3402 2 2 22 17 [Reporter]no spot (6B19)_PCR/2998 2 2 22 14 [Reporter]no spot (9F15)_PCR/2995 2 2 22 5 [Reporter]no spot (18B7)_PCR/2986 2 2 22 2 [Reporter]50% DMSO (CtrlB3)_PCR/2983 2 2 22 1 [Reporter]Cy3 lights carry-over (CtrlJ23)_PCR/2982 control_unknown_type 2 2 21 1 [Reporter]P10 (CtrlB23)_PCR/2960 2 2 20 1 [Reporter]P8 (CtrlN19)_PCR/2938 2 2 19 1 [Reporter]P7 (CtrlJ19)_PCR/2916 2 2 18 9 [Reporter]no spot (14B7)_PCR/2902 4 4 11 15 [Reporter]no spot (8P1)_PCR/7793 4 4 11 3 [Reporter]no spot (19P13)_PCR/7781 4 4 11 1 [Reporter]23s rRNA 1:16 (CtrlH9)_PCR/7779 control_biosequence 4 4 10 1 [Reporter]23s rRNA 1:4 (CtrlL9)_PCR/7757 control_biosequence 3 2 18 1 [Reporter]P6 (CtrlG19)_PCR/3398 3 2 17 21 [Reporter]no spot (2O15)_PCR/3396 4 4 9 1 [Reporter]23s rRNA 1:1 (CtrlP9)_PCR/7735 control_biosequence 4 4 8 20 [Reporter]no spot (3H5)_PCR/7732 3 2 17 1 [Reporter]P5 (CtrlC19)_PCR/3376 3 2 16 15 [Reporter]no spot (8C11)_PCR/3368 4 4 8 1 [Reporter]23s carry-over (CtrlD13)_PCR/7713 4 4 7 20 [Reporter]no spot (3D5)_PCR/7710 3 2 16 1 [Reporter]P4 (CtrlO15)_PCR/3354 4 4 7 12 [Reporter]no spot (10D21)_PCR/7702 4 4 7 11 [Reporter]no spot (11P17)_PCR/7701 3 2 15 1 [Reporter]P3 (CtrlK15)_PCR/3332 3 2 14 19 [Reporter]no spot (4K11)_PCR/3328 3 2 14 1 [Reporter]P1 (CtrlG15)_PCR/3310 3 2 13 15 [Reporter]no spot (8G7)_PCR/3302 2 2 18 3 [Reporter]no spot (19J23)_PCR/2896 2 2 18 1 [Reporter]P6 (CtrlF19)_PCR/2894 2 2 17 19 [Reporter]no spot (4F15)_PCR/2890 2 2 17 1 [Reporter]P5 (CtrlB19)_PCR/2872 2 2 16 1 [Reporter]P4 (CtrlN15)_PCR/2850 2 2 15 19 [Reporter]no spot (4N11)_PCR/2846 2 2 15 15 [Reporter]no spot (8N7)_PCR/2842 2 2 15 1 [Reporter]P3 (CtrlJ15)_PCR/2828 2 2 14 10 [Reporter]no spot (13F3)_PCR/2815 2 2 14 1 [Reporter]P1 (CtrlF15)_PCR/2806 4 4 7 1 [Reporter]16s rRNA 1:256 (CtrlH1)_PCR/7691 control_biosequence 4 4 6 10 [Reporter]no spot (12H17)_PCR/7678 4 4 6 1 [Reporter]16s rRNA 1:64 (CtrlL1)_PCR/7669 control_biosequence 4 4 5 19 [Reporter]no spot (4H1)_PCR/7665 4 4 5 2 [Reporter]no spot (20P5)_PCR/7648 4 4 5 1 [Reporter]16s rRNA 1:16 (CtrlP1)_PCR/7647 control_biosequence 4 4 4 16 [Reporter]no spot (6H21)_PCR/7640 3 2 13 1 [Reporter]23s rRNA 1:256 (CtrlO7)_PCR/3288 control_biosequence 4 4 4 14 [Reporter]no spot (8P17)_PCR/7638 4 4 4 1 [Reporter]16s rRNA 1:4 (CtrlD5)_PCR/7625 control_biosequence 3 2 12 4 [Reporter]no spot (18G19)_PCR/3269 3 2 12 1 [Reporter]23s rRNA 1:64 (CtrlC11)_PCR/3266 control_biosequence 4 4 3 17 [Reporter]no spot (5H21)_PCR/7619 4 4 3 12 [Reporter]no spot (10D17)_PCR/7614 3 2 11 10 [Reporter]no spot (12K23)_PCR/3253 4 4 3 1 [Reporter]16s rRNA 1:1 (CtrlH5)_PCR/7603 control_biosequence 3 2 11 1 [Reporter]23s rRNA 1:16 (CtrlG11)_PCR/3244 control_biosequence 3 2 10 19 [Reporter]no spot (4K7)_PCR/3240 3 2 10 1 [Reporter]23s rRNA 1:4 (CtrlK11)_PCR/3222 control_biosequence 3 2 9 21 [Reporter]no spot (2O7)_PCR/3220 3 2 9 6 [Reporter]no spot (16C19)_PCR/3205 3 2 9 1 [Reporter]23s rRNA 1:1 (CtrlO11)_PCR/3200 control_biosequence 2 2 13 1 [Reporter]23s rRNA 1:256 (CtrlN7)_PCR/2784 control_biosequence 2 2 12 12 [Reporter]no spot (11F3)_PCR/2773 2 2 12 1 [Reporter]23s rRNA 1:64 (CtrlB11)_PCR/2762 control_biosequence 1 1 1 2 [Reporter]no spot_PCR/8064 2 1 1 2 [Reporter]no spot_PCR/8064 3 1 1 2 [Reporter]no spot_PCR/8064 4 1 1 2 [Reporter]no spot_PCR/8064 1 2 1 2 [Reporter]no spot_PCR/8064 2 2 1 2 [Reporter]no spot_PCR/8064 3 2 1 2 [Reporter]no spot_PCR/8064 4 2 1 2 [Reporter]no spot_PCR/8064 1 3 1 2 [Reporter]no spot_PCR/8064 2 3 1 2 [Reporter]no spot_PCR/8064 3 3 1 2 [Reporter]no spot_PCR/8064 4 3 1 2 [Reporter]no spot_PCR/8064 1 4 1 2 [Reporter]no spot_PCR/8064 2 4 1 2 [Reporter]no spot_PCR/8064 3 4 1 2 [Reporter]no spot_PCR/8064 4 4 1 2 [Reporter]no spot_PCR/8064 1 1 23 2 [Reporter]no spot_PCR/8064 2 1 23 2 [Reporter]no spot_PCR/8064 3 1 23 2 [Reporter]no spot_PCR/8064 4 1 23 2 [Reporter]no spot_PCR/8064 1 2 23 2 [Reporter]no spot_PCR/8064 2 2 23 2 [Reporter]no spot_PCR/8064 3 2 23 2 [Reporter]no spot_PCR/8064 4 2 23 2 [Reporter]no spot_PCR/8064 1 3 23 2 [Reporter]no spot_PCR/8064 2 3 23 2 [Reporter]no spot_PCR/8064 3 3 23 2 [Reporter]no spot_PCR/8064 4 3 23 2 [Reporter]no spot_PCR/8064 1 4 23 2 [Reporter]no spot_PCR/8064 2 4 23 2 [Reporter]no spot_PCR/8064 3 4 23 2 [Reporter]no spot_PCR/8064 4 4 23 2 [Reporter]no spot_PCR/8064 2 2 11 1 [Reporter]23s rRNA 1:16 (CtrlF11)_PCR/2740 control_biosequence 4 4 23 1 [Reporter]Cy5 lights (CtrlP21)_PCR/8043 control_unknown_type 2 2 10 15 [Reporter]no spot (8J3)_PCR/2732 4 4 22 5 [Reporter]no spot (18D5)_PCR/8025 4 4 22 2 [Reporter]50% DMSO (CtrlD1)_PCR/8022 4 4 22 1 [Reporter]Cy3 lights carry-over (CtrlL21)_PCR/8021 control_unknown_type 2 2 10 1 [Reporter]23s rRNA 1:4 (CtrlJ11)_PCR/2718 control_biosequence 2 2 9 21 [Reporter]no spot (2N7)_PCR/2716 4 4 21 20 [Reporter]no spot (3L17)_PCR/8018 2 2 9 14 [Reporter]no spot (9B3)_PCR/2709 4 4 21 2 [Reporter]no spot (20P21)_PCR/8000 4 4 2 11 [Reporter]no spot (11L13)_PCR/7591 4 4 2 1 [Reporter]16s carry-over (CtrlL5)_PCR/7581 4 4 1 1 [Reporter]Cy3 lights (CtrlH21)_PCR/7560 control_unknown_type 3 4 23 14 [Reporter]no spot (9K13)_PCR/7551 3 2 8 20 [Reporter]no spot (3G7)_PCR/3197 3 4 23 11 [Reporter]no spot (12O9)_PCR/7548 3 4 23 1 [Reporter]Cy5 lights (CtrlO21)_PCR/7539 control_unknown_type 3 4 22 18 [Reporter]no spot (5G17)_PCR/7534 3 2 8 1 [Reporter]23s carry-over (CtrlC15)_PCR/3178 3 4 22 5 [Reporter]no spot (18C5)_PCR/7521 3 4 22 2 [Reporter]50% DMSO (CtrlC1)_PCR/7518 3 4 22 1 [Reporter]Cy3 lights carry-over (CtrlK21)_PCR/7517 control_unknown_type 3 4 21 21 [Reporter]no spot (2O17)_PCR/7515 3 4 21 18 [Reporter]no spot (5C17)_PCR/7512 3 4 21 16 [Reporter]no spot (7K13)_PCR/7510 3 2 7 1 [Reporter]16s rRNA 1:256 (CtrlG3)_PCR/3156 control_biosequence 3 2 6 1 [Reporter]16s rRNA 1:64 (CtrlK3)_PCR/3134 control_biosequence 3 2 5 2 [Reporter]no spot (20O7)_PCR/3113 3 2 5 1 [Reporter]16s rRNA 1:16 (CtrlO3)_PCR/3112 control_biosequence 3 2 4 20 [Reporter]no spot (3G3)_PCR/3109 2 2 9 1 [Reporter]23s rRNA 1:1 (CtrlN11)_PCR/2696 control_biosequence 2 2 8 1 [Reporter]23s carry-over (CtrlB15)_PCR/2674 2 2 7 20 [Reporter]no spot (3B7)_PCR/2671 2 2 7 1 [Reporter]16s rRNA 1:256 (CtrlF3)_PCR/2652 control_biosequence 2 2 6 16 [Reporter]no spot (6N23)_PCR/2645 2 2 6 1 [Reporter]16s rRNA 1:64 (CtrlJ3)_PCR/2630 control_biosequence 2 2 5 1 [Reporter]16s rRNA 1:16 (CtrlN3)_PCR/2608 control_biosequence 3 4 21 1 [Reporter]P10 (CtrlC21)_PCR/7495 3 4 20 1 [Reporter]P8 (CtrlO17)_PCR/7473 3 4 19 1 [Reporter]P7 (CtrlK17)_PCR/7451 3 2 4 2 [Reporter]no spot (20K7)_PCR/3091 3 2 4 1 [Reporter]16s rRNA 1:4 (CtrlC7)_PCR/3090 control_biosequence 3 2 3 16 [Reporter]no spot (6C23)_PCR/3083 3 4 18 1 [Reporter]P6 (CtrlG17)_PCR/7429 3 4 17 20 [Reporter]no spot (3K13)_PCR/7426 3 2 3 1 [Reporter]16s rRNA 1:1 (CtrlG7)_PCR/3068 control_biosequence 3 2 2 11 [Reporter]no spot (11K15)_PCR/3056 3 4 17 1 [Reporter]P5 (CtrlC17)_PCR/7407 3 2 2 1 [Reporter]16s carry-over (CtrlK7)_PCR/3046 3 2 1 10 [Reporter]no spot (12C15)_PCR/3033 3 2 1 4 [Reporter]no spot (18K7)_PCR/3027 3 2 1 1 [Reporter]Cy3 lights (CtrlG23)_PCR/3025 control_unknown_type 2 2 23 1 [Reporter]Cy5 lights (CtrlN23)_PCR/3004 control_unknown_type 2 4 21 1 [Reporter]P10 (CtrlB21)_PCR/6991 2 4 20 1 [Reporter]P8 (CtrlN17)_PCR/6969 2 4 19 1 [Reporter]P7 (CtrlJ17)_PCR/6947 2 2 4 1 [Reporter]16s rRNA 1:4 (CtrlB7)_PCR/2586 control_biosequence 2 2 3 20 [Reporter]no spot (3B3)_PCR/2583 2 4 18 1 [Reporter]P6 (CtrlF17)_PCR/6925 2 2 3 1 [Reporter]16s rRNA 1:1 (CtrlF7)_PCR/2564 control_biosequence 2 4 17 15 [Reporter]no spot (8F9)_PCR/6917 2 4 17 9 [Reporter]no spot (14N1)_PCR/6911 2 4 17 1 [Reporter]P5 (CtrlB17)_PCR/6903 2 2 2 1 [Reporter]16s carry-over (CtrlJ7)_PCR/2542 2 2 1 13 [Reporter]no spot (9N15)_PCR/2532 2 2 1 10 [Reporter]no spot (12B15)_PCR/2529 2 2 1 1 [Reporter]Cy3 lights (CtrlF23)_PCR/2521 control_unknown_type 1 2 23 1 [Reporter]Cy5 lights (CtrlM23)_PCR/2500 control_unknown_type 3 4 16 15 [Reporter]no spot (8C9)_PCR/7399 3 4 16 1 [Reporter]P4 (CtrlO13)_PCR/7385 3 4 15 1 [Reporter]P3 (CtrlK13)_PCR/7363 3 4 14 1 [Reporter]P1 (CtrlG13)_PCR/7341 3 4 13 21 [Reporter]no spot (2O9)_PCR/7339 3 4 13 3 [Reporter]no spot (19G17)_PCR/7321 3 4 13 1 [Reporter]23s rRNA 1:256 (CtrlO5)_PCR/7319 control_biosequence 2 4 16 1 [Reporter]P4 (CtrlN13)_PCR/6881 2 4 15 19 [Reporter]no spot (4N9)_PCR/6877 2 4 15 15 [Reporter]no spot (8N5)_PCR/6873 2 4 15 1 [Reporter]P3 (CtrlJ13)_PCR/6859 2 4 14 16 [Reporter]no spot (7N5)_PCR/6852 2 4 14 11 [Reporter]no spot (12J1)_PCR/6847 1 2 22 5 [Reporter]no spot (18A7)_PCR/2482 2 4 14 1 [Reporter]P1 (CtrlF13)_PCR/6837 1 2 22 2 [Reporter]50% DMSO (CtrlA3)_PCR/2479 1 2 22 1 [Reporter]Cy3 lights carry-over (CtrlI23)_PCR/2478 control_unknown_type 1 2 21 21 [Reporter]no spot (2M19)_PCR/2476 2 4 13 1 [Reporter]23s rRNA 1:256 (CtrlN5)_PCR/6815 control_biosequence 1 2 21 1 [Reporter]P10 (CtrlA23)_PCR/2456 2 4 12 15 [Reporter]no spot (8B5)_PCR/6807 1 2 20 1 [Reporter]P8 (CtrlM19)_PCR/2434 1 2 19 11 [Reporter]no spot (12M7)_PCR/2422 1 2 19 1 [Reporter]P7 (CtrlI19)_PCR/2412 4 1 23 5 [Reporter]no spot (18H8)_PCR/1999 4 1 23 1 [Reporter]Cy5 lights (CtrlP24)_PCR/1996 control_unknown_type 3 4 12 2 [Reporter]no spot (20K13)_PCR/7298 3 4 12 1 [Reporter]23s rRNA 1:64 (CtrlC9)_PCR/7297 control_biosequence 4 1 22 14 [Reporter]no spot (9H16)_PCR/1987 4 1 22 9 [Reporter]no spot (14D12)_PCR/1982 4 1 22 2 [Reporter]50% DMSO (CtrlD4)_PCR/1975 4 1 22 1 [Reporter]Cy3 lights carry-over (CtrlL24)_PCR/1974 control_unknown_type 4 1 21 22 [Reporter]no spot (1D24)_PCR/1973 3 4 11 1 [Reporter]23s rRNA 1:16 (CtrlG9)_PCR/7275 control_biosequence 3 4 10 18 [Reporter]no spot (5G5)_PCR/7270 3 4 10 11 [Reporter]no spot (11K21)_PCR/7263 3 4 10 9 [Reporter]no spot (13C21)_PCR/7261 4 1 21 1 [Reporter]P10 (CtrlD24)_PCR/1952 3 4 10 1 [Reporter]23s rRNA 1:4 (CtrlK9)_PCR/7253 control_biosequence 3 4 9 12 [Reporter]no spot (10K21)_PCR/7242 3 4 9 7 [Reporter]no spot (15G17)_PCR/7237 4 1 20 1 [Reporter]P8 (CtrlP20)_PCR/1930 3 4 9 1 [Reporter]23s rRNA 1:1 (CtrlO9)_PCR/7231 control_biosequence 4 1 19 20 [Reporter]no spot (3D20)_PCR/1927 4 1 19 19 [Reporter]no spot (4P16)_PCR/1926 4 1 19 15 [Reporter]no spot (8P12)_PCR/1922 3 4 8 20 [Reporter]no spot (3G5)_PCR/7228 3 4 8 2 [Reporter]no spot (20K9)_PCR/7210 4 1 19 1 [Reporter]P7 (CtrlL20)_PCR/1908 4 1 18 18 [Reporter]no spot (5H16)_PCR/1903 3 4 8 1 [Reporter]23s carry-over (CtrlC13)_PCR/7209 3 4 7 20 [Reporter]no spot (3C5)_PCR/7206 2 4 12 5 [Reporter]no spot (17J17)_PCR/6797 2 4 12 1 [Reporter]23s rRNA 1:64 (CtrlB9)_PCR/6793 control_biosequence 2 4 11 15 [Reporter]no spot (8N1)_PCR/6785 2 4 11 1 [Reporter]23s rRNA 1:16 (CtrlF9)_PCR/6771 control_biosequence 1 2 18 9 [Reporter]no spot (14A7)_PCR/2398 1 2 18 5 [Reporter]no spot (18A3)_PCR/2394 1 2 18 1 [Reporter]P6 (CtrlE19)_PCR/2390 2 4 10 1 [Reporter]23s rRNA 1:4 (CtrlJ9)_PCR/6749 control_biosequence 1 2 17 19 [Reporter]no spot (4E15)_PCR/2386 1 2 17 13 [Reporter]no spot (10M7)_PCR/2380 1 2 17 3 [Reporter]no spot (19E23)_PCR/2370 2 4 9 1 [Reporter]23s rRNA 1:1 (CtrlN9)_PCR/6727 control_biosequence 2 4 8 20 [Reporter]no spot (3F5)_PCR/6724 1 2 17 1 [Reporter]P5 (CtrlA19)_PCR/2368 1 2 16 15 [Reporter]no spot (8A11)_PCR/2360 1 2 16 11 [Reporter]no spot (12A7)_PCR/2356 2 4 8 1 [Reporter]23s carry-over (CtrlB13)_PCR/6705 1 2 16 1 [Reporter]P4 (CtrlM15)_PCR/2346 1 2 15 17 [Reporter]no spot (6E11)_PCR/2340 1 2 15 7 [Reporter]no spot (15M23)_PCR/2330 1 2 15 5 [Reporter]no spot (17E23)_PCR/2328 1 2 15 1 [Reporter]P3 (CtrlI15)_PCR/2324 1 2 14 9 [Reporter]no spot (14A3)_PCR/2310 1 2 14 8 [Reporter]no spot (14M23)_PCR/2309 1 2 14 1 [Reporter]P1 (CtrlE15)_PCR/2302 3 4 7 8 [Reporter]no spot (14C17)_PCR/7194 4 1 18 1 [Reporter]P6 (CtrlH20)_PCR/1886 3 4 7 1 [Reporter]16s rRNA 1:256 (CtrlG1)_PCR/7187 control_biosequence 3 4 6 19 [Reporter]no spot (4K1)_PCR/7183 4 1 17 16 [Reporter]no spot (7L12)_PCR/1879 4 1 17 1 [Reporter]P5 (CtrlD20)_PCR/1864 3 4 6 1 [Reporter]16s rRNA 1:64 (CtrlK1)_PCR/7165 control_biosequence 4 1 16 17 [Reporter]no spot (6L12)_PCR/1858 4 1 16 7 [Reporter]no spot (16D4)_PCR/1848 4 1 16 4 [Reporter]no spot (18H24)_PCR/1845 4 1 16 1 [Reporter]P4 (CtrlP16)_PCR/1842 3 4 5 2 [Reporter]no spot (20O5)_PCR/7144 3 4 5 1 [Reporter]16s rRNA 1:16 (CtrlO1)_PCR/7143 control_biosequence 4 1 15 11 [Reporter]no spot (12P4)_PCR/1830 3 4 4 12 [Reporter]no spot (10G17)_PCR/7132 4 1 15 1 [Reporter]P3 (CtrlL16)_PCR/1820 3 4 4 1 [Reporter]16s rRNA 1:4 (CtrlC5)_PCR/7121 control_biosequence 4 1 14 22 [Reporter]no spot (1H16)_PCR/1819 3 4 3 3 [Reporter]no spot (19O5)_PCR/7101 2 4 7 1 [Reporter]16s rRNA 1:256 (CtrlF1)_PCR/6683 control_biosequence 2 4 6 7 [Reporter]no spot (15J13)_PCR/6667 2 4 6 1 [Reporter]16s rRNA 1:64 (CtrlJ1)_PCR/6661 control_biosequence 1 2 13 20 [Reporter]no spot (3I11)_PCR/2299 2 4 5 12 [Reporter]no spot (10J17)_PCR/6650 1 2 13 7 [Reporter]no spot (15E23)_PCR/2286 1 2 13 1 [Reporter]23s rRNA 1:256 (CtrlM7)_PCR/2280 control_biosequence 2 4 5 1 [Reporter]16s rRNA 1:16 (CtrlN1)_PCR/6639 control_biosequence 2 4 4 1 [Reporter]16s rRNA 1:4 (CtrlB5)_PCR/6617 control_biosequence 1 2 12 1 [Reporter]23s rRNA 1:64 (CtrlA11)_PCR/2258 control_biosequence 1 2 11 1 [Reporter]23s rRNA 1:16 (CtrlE11)_PCR/2236 control_biosequence 1 2 10 3 [Reporter]no spot (19I15)_PCR/2216 1 2 10 1 [Reporter]23s rRNA 1:4 (CtrlI11)_PCR/2214 control_biosequence 1 2 9 19 [Reporter]no spot (4E7)_PCR/2210 4 1 14 1 [Reporter]P1 (CtrlH16)_PCR/1798 3 4 3 1 [Reporter]16s rRNA 1:1 (CtrlG5)_PCR/7099 control_biosequence 4 1 13 9 [Reporter]no spot (13P24)_PCR/1784 4 1 13 7 [Reporter]no spot (15H24)_PCR/1782 3 4 2 10 [Reporter]no spot (12G13)_PCR/7086 4 1 13 1 [Reporter]23s rRNA 1:256 (CtrlP8)_PCR/1776 control_biosequence 3 4 2 1 [Reporter]16s carry-over (CtrlK5)_PCR/7077 4 1 12 1 [Reporter]23s rRNA 1:64 (CtrlD12)_PCR/1754 control_biosequence 4 1 11 20 [Reporter]no spot (3D12)_PCR/1751 3 4 1 1 [Reporter]Cy3 lights (CtrlG21)_PCR/7056 control_unknown_type 4 1 11 16 [Reporter]no spot (7D8)_PCR/1747 4 1 11 15 [Reporter]no spot (8P4)_PCR/1746 4 1 11 10 [Reporter]no spot (12L24)_PCR/1741 4 1 11 1 [Reporter]23s rRNA 1:16 (CtrlH12)_PCR/1732 control_biosequence 2 4 23 1 [Reporter]Cy5 lights (CtrlN21)_PCR/7035 control_unknown_type 4 1 10 1 [Reporter]23s rRNA 1:4 (CtrlL12)_PCR/1710 control_biosequence 2 4 22 2 [Reporter]50% DMSO (CtrlB1)_PCR/7014 2 4 22 1 [Reporter]Cy3 lights carry-over (CtrlJ21)_PCR/7013 control_unknown_type 2 4 3 4 [Reporter]no spot (18B9)_PCR/6598 2 4 3 2 [Reporter]no spot (20F5)_PCR/6596 2 4 3 1 [Reporter]16s rRNA 1:1 (CtrlF5)_PCR/6595 control_biosequence 2 4 2 12 [Reporter]no spot (10N13)_PCR/6584 2 4 2 1 [Reporter]16s carry-over (CtrlJ5)_PCR/6573 2 4 1 1 [Reporter]Cy3 lights (CtrlF21)_PCR/6552 control_unknown_type 1 2 9 1 [Reporter]23s rRNA 1:1 (CtrlM11)_PCR/2192 control_biosequence 1 4 23 1 [Reporter]Cy5 lights (CtrlM21)_PCR/6531 control_unknown_type 1 2 8 1 [Reporter]23s carry-over (CtrlA15)_PCR/2170 1 4 22 10 [Reporter]no spot (13E9)_PCR/6518 1 4 22 2 [Reporter]50% DMSO (CtrlA1)_PCR/6510 1 4 22 1 [Reporter]Cy3 lights carry-over (CtrlI21)_PCR/6509 control_unknown_type 1 2 7 1 [Reporter]16s rRNA 1:256 (CtrlE3)_PCR/2148 control_biosequence 1 2 6 1 [Reporter]16s rRNA 1:64 (CtrlI3)_PCR/2126 control_biosequence 1 2 5 22 [Reporter]no spot (1A7)_PCR/2125 1 2 5 1 [Reporter]16s rRNA 1:16 (CtrlM3)_PCR/2104 control_biosequence 4 1 9 1 [Reporter]23s rRNA 1:1 (CtrlP12)_PCR/1688 control_biosequence 4 1 8 1 [Reporter]23s carry-over (CtrlD16)_PCR/1666 4 1 7 10 [Reporter]no spot (12L20)_PCR/1653 4 1 7 2 [Reporter]no spot (20H12)_PCR/1645 4 1 7 1 [Reporter]16s rRNA 1:256 (CtrlH4)_PCR/1644 control_biosequence 4 1 6 19 [Reporter]no spot (4L4)_PCR/1640 4 1 6 1 [Reporter]16s rRNA 1:64 (CtrlL4)_PCR/1622 control_biosequence 4 1 5 1 [Reporter]16s rRNA 1:16 (CtrlP4)_PCR/1600 control_biosequence 1 4 21 10 [Reporter]no spot (13A9)_PCR/6496 1 4 21 1 [Reporter]P10 (CtrlA21)_PCR/6487 1 4 20 19 [Reporter]no spot (4A17)_PCR/6483 1 4 20 1 [Reporter]P8 (CtrlM17)_PCR/6465 1 4 19 13 [Reporter]no spot (10E9)_PCR/6455 1 4 19 1 [Reporter]P7 (CtrlI17)_PCR/6443 1 2 4 4 [Reporter]no spot (18E11)_PCR/2085 1 2 4 1 [Reporter]16s rRNA 1:4 (CtrlA7)_PCR/2082 control_biosequence 1 2 3 14 [Reporter]no spot (8I19)_PCR/2073 1 4 18 8 [Reporter]no spot (15M1)_PCR/6428 1 4 18 1 [Reporter]P6 (CtrlE17)_PCR/6421 1 2 3 1 [Reporter]16s rRNA 1:1 (CtrlE7)_PCR/2060 control_biosequence 1 4 17 16 [Reporter]no spot (7I9)_PCR/6414 1 2 2 1 [Reporter]16s carry-over (CtrlI7)_PCR/2038 1 2 1 22 [Reporter]no spot (1A3)_PCR/2037 1 2 1 3 [Reporter]no spot (19E7)_PCR/2018 1 2 1 1 [Reporter]Cy3 lights (CtrlE23)_PCR/2017 control_unknown_type 4 1 23 19 [Reporter]no spot (4P20)_PCR/2013 4 1 23 13 [Reporter]no spot (10H16)_PCR/2007 4 3 21 3 [Reporter]no spot (20H2)_PCR/5985 4 3 21 1 [Reporter]P10 (CtrlD22)_PCR/5983 4 3 20 16 [Reporter]no spot (7H14)_PCR/5976 4 3 20 14 [Reporter]no spot (9P10)_PCR/5974 4 3 20 11 [Reporter]no spot (12D10)_PCR/5971 4 3 20 1 [Reporter]P8 (CtrlP18)_PCR/5961 4 3 19 18 [Reporter]no spot (5L14)_PCR/5956 4 1 4 20 [Reporter]no spot (3H4)_PCR/1597 4 3 19 3 [Reporter]no spot (19P22)_PCR/5942 4 3 19 1 [Reporter]P7 (CtrlL18)_PCR/5940 4 1 4 2 [Reporter]no spot (20L8)_PCR/1579 4 1 4 1 [Reporter]16s rRNA 1:4 (CtrlD8)_PCR/1578 control_biosequence 4 3 18 5 [Reporter]no spot (18D2)_PCR/5922 4 3 18 1 [Reporter]P6 (CtrlH18)_PCR/5918 4 1 3 3 [Reporter]no spot (19P8)_PCR/1558 4 1 3 1 [Reporter]16s rRNA 1:1 (CtrlH8)_PCR/1556 control_biosequence 4 1 2 1 [Reporter]16s carry-over (CtrlL8)_PCR/1534 4 1 1 1 [Reporter]Cy3 lights (CtrlH24)_PCR/1513 control_unknown_type 1 4 17 1 [Reporter]P5 (CtrlA17)_PCR/6399 1 4 16 1 [Reporter]P4 (CtrlM13)_PCR/6377 1 4 15 19 [Reporter]no spot (4M9)_PCR/6373 1 4 15 11 [Reporter]no spot (12M1)_PCR/6365 1 4 15 1 [Reporter]P3 (CtrlI13)_PCR/6355 1 4 14 1 [Reporter]P1 (CtrlE13)_PCR/6333 1 4 13 15 [Reporter]no spot (8E5)_PCR/6325 1 4 13 6 [Reporter]no spot (16A21)_PCR/6316 1 4 13 1 [Reporter]23s rRNA 1:256 (CtrlM5)_PCR/6311 control_biosequence 1 4 12 20 [Reporter]no spot (3E9)_PCR/6308 4 3 17 4 [Reporter]no spot (18L22)_PCR/5899 4 3 17 1 [Reporter]P5 (CtrlD18)_PCR/5896 4 3 16 1 [Reporter]P4 (CtrlP14)_PCR/5874 4 3 15 1 [Reporter]P3 (CtrlL14)_PCR/5852 3 1 23 1 [Reporter]Cy5 lights (CtrlO24)_PCR/1492 control_unknown_type 4 3 14 19 [Reporter]no spot (4L10)_PCR/5848 4 3 14 13 [Reporter]no spot (10D6)_PCR/5842 4 3 14 1 [Reporter]P1 (CtrlH14)_PCR/5830 3 1 22 2 [Reporter]50% DMSO (CtrlC4)_PCR/1471 3 1 22 1 [Reporter]Cy3 lights carry-over (CtrlK24)_PCR/1470 control_unknown_type 3 1 21 17 [Reporter]no spot (6O16)_PCR/1464 3 1 21 14 [Reporter]no spot (9C16)_PCR/1461 4 3 13 5 [Reporter]no spot (17P18)_PCR/5812 4 3 13 4 [Reporter]no spot (18L18)_PCR/5811 4 3 13 1 [Reporter]23s rRNA 1:256 (CtrlP6)_PCR/5808 control_biosequence 3 1 21 1 [Reporter]P10 (CtrlC24)_PCR/1448 3 1 20 1 [Reporter]P8 (CtrlO20)_PCR/1426 3 1 19 1 [Reporter]P7 (CtrlK20)_PCR/1404 1 4 12 4 [Reporter]no spot (18E17)_PCR/6292 1 4 12 1 [Reporter]23s rRNA 1:64 (CtrlA9)_PCR/6289 control_biosequence 1 4 11 4 [Reporter]no spot (18A17)_PCR/6270 1 4 11 1 [Reporter]23s rRNA 1:16 (CtrlE9)_PCR/6267 control_biosequence 1 4 10 1 [Reporter]23s rRNA 1:4 (CtrlI9)_PCR/6245 control_biosequence 1 4 9 2 [Reporter]no spot (20M9)_PCR/6224 1 4 9 1 [Reporter]23s rRNA 1:1 (CtrlM9)_PCR/6223 control_biosequence 1 4 8 20 [Reporter]no spot (3E5)_PCR/6220 1 4 8 1 [Reporter]23s carry-over (CtrlA13)_PCR/6201 4 3 12 10 [Reporter]no spot (12P22)_PCR/5795 4 3 12 1 [Reporter]23s rRNA 1:64 (CtrlD10)_PCR/5786 control_biosequence 4 3 11 17 [Reporter]no spot (6H6)_PCR/5780 4 3 11 1 [Reporter]23s rRNA 1:16 (CtrlH10)_PCR/5764 control_biosequence 3 1 18 9 [Reporter]no spot (14C8)_PCR/1390 4 3 10 1 [Reporter]23s rRNA 1:4 (CtrlL10)_PCR/5742 control_biosequence 4 3 9 21 [Reporter]no spot (2P6)_PCR/5740 3 1 18 5 [Reporter]no spot (18C4)_PCR/1386 3 1 18 1 [Reporter]P6 (CtrlG20)_PCR/1382 4 3 9 14 [Reporter]no spot (9D2)_PCR/5733 4 3 9 12 [Reporter]no spot (10L22)_PCR/5731 4 3 9 1 [Reporter]23s rRNA 1:1 (CtrlP10)_PCR/5720 control_biosequence 3 1 17 1 [Reporter]P5 (CtrlC20)_PCR/1360 4 3 8 21 [Reporter]no spot (2L6)_PCR/5718 4 3 8 14 [Reporter]no spot (8P22)_PCR/5711 3 1 16 1 [Reporter]P4 (CtrlO16)_PCR/1338 3 1 15 13 [Reporter]no spot (10G8)_PCR/1328 3 1 15 1 [Reporter]P3 (CtrlK16)_PCR/1316 3 1 14 13 [Reporter]no spot (10C8)_PCR/1306 1 4 7 1 [Reporter]16s rRNA 1:256 (CtrlE1)_PCR/6179 control_biosequence 1 4 6 1 [Reporter]16s rRNA 1:64 (CtrlI1)_PCR/6157 control_biosequence 1 4 5 2 [Reporter]no spot (20M5)_PCR/6136 1 4 5 1 [Reporter]16s rRNA 1:16 (CtrlM1)_PCR/6135 control_biosequence 1 4 4 1 [Reporter]16s rRNA 1:4 (CtrlA5)_PCR/6113 control_biosequence 4 3 8 1 [Reporter]23s carry-over (CtrlD14)_PCR/5698 4 3 7 21 [Reporter]no spot (2H6)_PCR/5696 4 3 7 20 [Reporter]no spot (3D6)_PCR/5695 4 3 7 1 [Reporter]16s rRNA 1:256 (CtrlH2)_PCR/5676 control_biosequence 4 3 6 12 [Reporter]no spot (10P18)_PCR/5665 4 3 6 10 [Reporter]no spot (12H18)_PCR/5663 4 3 6 1 [Reporter]16s rRNA 1:64 (CtrlL2)_PCR/5654 control_biosequence 3 1 14 1 [Reporter]P1 (CtrlG16)_PCR/1294 4 3 5 17 [Reporter]no spot (5P22)_PCR/5648 3 1 13 16 [Reporter]no spot (7K8)_PCR/1287 4 3 5 1 [Reporter]16s rRNA 1:16 (CtrlP2)_PCR/5632 control_biosequence 3 1 13 1 [Reporter]23s rRNA 1:256 (CtrlO8)_PCR/1272 control_biosequence 4 3 4 11 [Reporter]no spot (11D18)_PCR/5620 4 3 4 1 [Reporter]16s rRNA 1:4 (CtrlD6)_PCR/5610 control_biosequence 3 1 12 9 [Reporter]no spot (13K24)_PCR/1258 3 1 12 1 [Reporter]23s rRNA 1:64 (CtrlC12)_PCR/1250 control_biosequence 4 3 3 19 [Reporter]no spot (3P22)_PCR/5606 3 1 11 19 [Reporter]no spot (4O8)_PCR/1246 3 1 11 1 [Reporter]23s rRNA 1:16 (CtrlG12)_PCR/1228 control_biosequence 3 1 10 22 [Reporter]no spot (1G12)_PCR/1227 3 1 10 1 [Reporter]23s rRNA 1:4 (CtrlK12)_PCR/1206 control_biosequence 1 4 3 9 [Reporter]no spot (13E13)_PCR/6099 1 4 3 1 [Reporter]16s rRNA 1:1 (CtrlE5)_PCR/6091 control_biosequence 1 4 2 1 [Reporter]16s carry-over (CtrlI5)_PCR/6069 1 4 1 1 [Reporter]Cy3 lights (CtrlE21)_PCR/6048 control_unknown_type 4 3 23 8 [Reporter]no spot (15D10)_PCR/6033 4 3 23 1 [Reporter]Cy5 lights (CtrlP22)_PCR/6027 control_unknown_type 4 3 22 2 [Reporter]50% DMSO (CtrlD2)_PCR/6006 4 3 22 1 [Reporter]Cy3 lights carry-over (CtrlL22)_PCR/6005 control_unknown_type 4 3 3 9 [Reporter]no spot (13H14)_PCR/5596 4 3 3 1 [Reporter]16s rRNA 1:1 (CtrlH6)_PCR/5588 control_biosequence 4 3 2 9 [Reporter]no spot (13D14)_PCR/5574 4 3 2 1 [Reporter]16s carry-over (CtrlL6)_PCR/5566 4 3 1 20 [Reporter]no spot (2L22)_PCR/5563 4 3 1 1 [Reporter]Cy3 lights (CtrlH22)_PCR/5545 control_unknown_type 3 1 9 1 [Reporter]23s rRNA 1:1 (CtrlO12)_PCR/1184 control_biosequence 3 3 23 8 [Reporter]no spot (15C10)_PCR/5530 3 3 23 1 [Reporter]Cy5 lights (CtrlO22)_PCR/5524 control_unknown_type 3 1 8 1 [Reporter]23s carry-over (CtrlC16)_PCR/1162 3 3 22 17 [Reporter]no spot (6C18)_PCR/5518 3 3 22 15 [Reporter]no spot (8K14)_PCR/5516 3 3 22 9 [Reporter]no spot (14C10)_PCR/5510 3 1 7 16 [Reporter]no spot (7C4)_PCR/1155 3 1 7 13 [Reporter]no spot (9G24)_PCR/1152 3 3 22 2 [Reporter]50% DMSO (CtrlC2)_PCR/5503 3 3 22 1 [Reporter]Cy3 lights carry-over (CtrlK22)_PCR/5502 control_unknown_type 3 1 7 1 [Reporter]16s rRNA 1:256 (CtrlG4)_PCR/1140 control_biosequence 3 1 6 13 [Reporter]no spot (9C24)_PCR/1130 3 1 6 12 [Reporter]no spot (10O20)_PCR/1129 3 1 6 1 [Reporter]16s rRNA 1:64 (CtrlK4)_PCR/1118 control_biosequence 3 1 5 12 [Reporter]no spot (10K20)_PCR/1107 3 1 5 7 [Reporter]no spot (15G16)_PCR/1102 3 3 21 1 [Reporter]P10 (CtrlC22)_PCR/5480 3 3 20 1 [Reporter]P8 (CtrlO18)_PCR/5458 3 1 5 4 [Reporter]no spot (18K12)_PCR/1099 3 1 5 2 [Reporter]no spot (20O8)_PCR/1097 3 1 5 1 [Reporter]16s rRNA 1:16 (CtrlO4)_PCR/1096 control_biosequence 3 1 4 20 [Reporter]no spot (3G4)_PCR/1093 3 3 19 1 [Reporter]P7 (CtrlK18)_PCR/5436 3 1 4 1 [Reporter]16s rRNA 1:4 (CtrlC8)_PCR/1074 control_biosequence 3 3 18 5 [Reporter]no spot (18C2)_PCR/5418 3 3 18 1 [Reporter]P6 (CtrlG18)_PCR/5414 3 1 3 2 [Reporter]no spot (20G8)_PCR/1053 3 1 3 1 [Reporter]16s rRNA 1:1 (CtrlG8)_PCR/1052 control_biosequence 3 1 2 21 [Reporter]no spot (2C4)_PCR/1050 3 3 17 11 [Reporter]no spot (12G6)_PCR/5402 3 1 2 1 [Reporter]16s carry-over (CtrlK8)_PCR/1030 3 1 1 16 [Reporter]no spot (6K20)_PCR/1023 3 1 1 4 [Reporter]no spot (18K8)_PCR/1011 2 3 22 2 [Reporter]50% DMSO (CtrlB2)_PCR/4999 3 1 1 1 [Reporter]Cy3 lights (CtrlG24)_PCR/1009 control_unknown_type 2 3 22 1 [Reporter]Cy3 lights carry-over (CtrlJ22)_PCR/4998 control_unknown_type 2 3 21 1 [Reporter]P10 (CtrlB22)_PCR/4976 2 3 20 9 [Reporter]no spot (14J6)_PCR/4962 2 3 20 1 [Reporter]P8 (CtrlN18)_PCR/4954 2 3 19 18 [Reporter]no spot (5J14)_PCR/4949 2 3 19 17 [Reporter]no spot (6F14)_PCR/4948 2 3 19 1 [Reporter]P7 (CtrlJ18)_PCR/4932 2 3 18 13 [Reporter]no spot (10B10)_PCR/4922 2 3 18 1 [Reporter]P6 (CtrlF18)_PCR/4910 3 3 17 1 [Reporter]P5 (CtrlC18)_PCR/5392 3 3 16 12 [Reporter]no spot (11G6)_PCR/5381 3 3 16 1 [Reporter]P4 (CtrlO14)_PCR/5370 3 3 15 1 [Reporter]P3 (CtrlK14)_PCR/5348 3 3 14 1 [Reporter]P1 (CtrlG14)_PCR/5326 3 3 13 1 [Reporter]23s rRNA 1:256 (CtrlO6)_PCR/5304 control_biosequence 2 1 23 1 [Reporter]Cy5 lights (CtrlN24)_PCR/988 control_unknown_type 2 1 22 5 [Reporter]no spot (18B8)_PCR/970 2 3 17 2 [Reporter]no spot (20N18)_PCR/4889 2 3 17 1 [Reporter]P5 (CtrlB18)_PCR/4888 2 1 22 2 [Reporter]50% DMSO (CtrlB4)_PCR/967 2 1 22 1 [Reporter]Cy3 lights carry-over (CtrlJ24)_PCR/966 control_unknown_type 2 3 16 11 [Reporter]no spot (12B6)_PCR/4876 2 3 16 2 [Reporter]no spot (20J18)_PCR/4867 2 3 16 1 [Reporter]P4 (CtrlN14)_PCR/4866 2 1 21 1 [Reporter]P10 (CtrlB24)_PCR/944 2 3 15 1 [Reporter]P3 (CtrlJ14)_PCR/4844 2 1 20 1 [Reporter]P8 (CtrlN20)_PCR/922 2 3 14 11 [Reporter]no spot (12J2)_PCR/4832 2 3 14 1 [Reporter]P1 (CtrlF14)_PCR/4822 2 1 19 1 [Reporter]P7 (CtrlJ20)_PCR/900 2 3 13 1 [Reporter]23s rRNA 1:256 (CtrlN6)_PCR/4800 control_biosequence 3 3 12 16 [Reporter]no spot (7G6)_PCR/5297 3 3 12 15 [Reporter]no spot (8C6)_PCR/5296 3 3 12 1 [Reporter]23s rRNA 1:64 (CtrlC10)_PCR/5282 control_biosequence 3 3 11 15 [Reporter]no spot (8O2)_PCR/5274 3 3 11 1 [Reporter]23s rRNA 1:16 (CtrlG10)_PCR/5260 control_biosequence 3 3 10 1 [Reporter]23s rRNA 1:4 (CtrlK10)_PCR/5238 control_biosequence 3 3 9 12 [Reporter]no spot (10K22)_PCR/5227 3 3 9 1 [Reporter]23s rRNA 1:1 (CtrlO10)_PCR/5216 control_biosequence 3 3 8 20 [Reporter]no spot (3G6)_PCR/5213 3 3 8 18 [Reporter]no spot (5O2)_PCR/5211 2 1 18 1 [Reporter]P6 (CtrlF20)_PCR/878 2 3 12 1 [Reporter]23s rRNA 1:64 (CtrlB10)_PCR/4778 control_biosequence 2 3 11 19 [Reporter]no spot (4N6)_PCR/4774 2 1 17 4 [Reporter]no spot (18J24)_PCR/859 2 1 17 1 [Reporter]P5 (CtrlB20)_PCR/856 2 3 11 1 [Reporter]23s rRNA 1:16 (CtrlF10)_PCR/4756 control_biosequence 2 1 16 1 [Reporter]P4 (CtrlN16)_PCR/834 2 3 10 1 [Reporter]23s rRNA 1:4 (CtrlJ10)_PCR/4734 control_biosequence 2 1 15 1 [Reporter]P3 (CtrlJ16)_PCR/812 2 3 9 10 [Reporter]no spot (12B22)_PCR/4721 2 3 9 8 [Reporter]no spot (14J18)_PCR/4719 2 3 9 1 [Reporter]23s rRNA 1:1 (CtrlN10)_PCR/4712 control_biosequence 2 3 8 20 [Reporter]no spot (3F6)_PCR/4709 3 3 8 1 [Reporter]23s carry-over (CtrlC14)_PCR/5194 3 3 7 20 [Reporter]no spot (3C6)_PCR/5191 3 3 7 19 [Reporter]no spot (4O2)_PCR/5190 3 3 7 1 [Reporter]16s rRNA 1:256 (CtrlG2)_PCR/5172 control_biosequence 3 3 6 10 [Reporter]no spot (12G18)_PCR/5159 3 3 6 1 [Reporter]16s rRNA 1:64 (CtrlK2)_PCR/5150 control_biosequence 3 3 5 13 [Reporter]no spot (9O18)_PCR/5140 3 3 5 12 [Reporter]no spot (10K18)_PCR/5139 3 3 5 1 [Reporter]16s rRNA 1:16 (CtrlO2)_PCR/5128 control_biosequence 3 3 4 8 [Reporter]no spot (14G14)_PCR/5113 3 3 4 1 [Reporter]16s rRNA 1:4 (CtrlC6)_PCR/5106 control_biosequence 2 1 14 1 [Reporter]P1 (CtrlF16)_PCR/790 2 3 8 1 [Reporter]23s carry-over (CtrlB14)_PCR/4690 2 3 7 20 [Reporter]no spot (3B6)_PCR/4687 2 1 13 1 [Reporter]23s rRNA 1:256 (CtrlN8)_PCR/768 control_biosequence 2 1 12 13 [Reporter]no spot (10J4)_PCR/758 2 3 7 1 [Reporter]16s rRNA 1:256 (CtrlF2)_PCR/4668 control_biosequence 2 1 12 1 [Reporter]23s rRNA 1:64 (CtrlB12)_PCR/746 control_biosequence 2 3 6 1 [Reporter]16s rRNA 1:64 (CtrlJ2)_PCR/4646 control_biosequence 2 1 11 1 [Reporter]23s rRNA 1:16 (CtrlF12)_PCR/724 control_biosequence 2 3 5 15 [Reporter]no spot (7F22)_PCR/4638 2 3 5 13 [Reporter]no spot (9N18)_PCR/4636 2 3 5 1 [Reporter]16s rRNA 1:16 (CtrlN2)_PCR/4624 control_biosequence 2 1 10 1 [Reporter]23s rRNA 1:4 (CtrlJ12)_PCR/702 control_biosequence 2 3 4 1 [Reporter]16s rRNA 1:4 (CtrlB6)_PCR/4602 control_biosequence 3 3 3 1 [Reporter]16s rRNA 1:1 (CtrlG6)_PCR/5084 control_biosequence 3 3 2 13 [Reporter]no spot (9C18)_PCR/5074 3 3 2 1 [Reporter]16s carry-over (CtrlK6)_PCR/5062 3 3 1 21 [Reporter]no spot (1O22)_PCR/5060 3 3 1 20 [Reporter]no spot (2K22)_PCR/5059 3 3 1 1 [Reporter]Cy3 lights (CtrlG22)_PCR/5041 control_unknown_type 2 3 23 9 [Reporter]no spot (14F10)_PCR/5027 2 3 23 1 [Reporter]Cy5 lights (CtrlN22)_PCR/5020 control_unknown_type 2 1 9 1 [Reporter]23s rRNA 1:1 (CtrlN12)_PCR/680 control_biosequence 2 1 8 14 [Reporter]no spot (8N24)_PCR/671 2 3 3 1 [Reporter]16s rRNA 1:1 (CtrlF6)_PCR/4580 control_biosequence 2 1 8 1 [Reporter]23s carry-over (CtrlB16)_PCR/658 2 3 2 1 [Reporter]16s carry-over (CtrlJ6)_PCR/4558 2 1 7 4 [Reporter]no spot (18B16)_PCR/639 2 1 7 1 [Reporter]16s rRNA 1:256 (CtrlF4)_PCR/636 control_biosequence 2 3 1 1 [Reporter]Cy3 lights (CtrlF22)_PCR/4537 control_unknown_type 2 1 6 1 [Reporter]16s rRNA 1:64 (CtrlJ4)_PCR/614 control_biosequence 1 3 23 6 [Reporter]no spot (17I6)_PCR/4520 1 3 23 5 [Reporter]no spot (18E6)_PCR/4519 1 3 23 1 [Reporter]Cy5 lights (CtrlM22)_PCR/4516 control_unknown_type 2 1 5 1 [Reporter]16s rRNA 1:16 (CtrlN4)_PCR/592 control_biosequence 2 1 4 20 [Reporter]no spot (3F4)_PCR/589 2 1 4 13 [Reporter]no spot (9J20)_PCR/582 1 3 22 2 [Reporter]50% DMSO (CtrlA2)_PCR/4495 1 3 22 1 [Reporter]Cy3 lights carry-over (CtrlI22)_PCR/4494 control_unknown_type 2 1 4 1 [Reporter]16s rRNA 1:4 (CtrlB8)_PCR/570 control_biosequence 1 3 21 1 [Reporter]P10 (CtrlA22)_PCR/4472 2 1 3 6 [Reporter]no spot (16J12)_PCR/553 2 1 3 4 [Reporter]no spot (18B12)_PCR/551 2 1 3 1 [Reporter]16s rRNA 1:1 (CtrlF8)_PCR/548 control_biosequence 1 3 20 1 [Reporter]P8 (CtrlM18)_PCR/4450 2 1 2 1 [Reporter]16s carry-over (CtrlJ8)_PCR/526 1 3 19 1 [Reporter]P7 (CtrlI18)_PCR/4428 1 3 18 16 [Reporter]no spot (7M10)_PCR/4421 2 1 1 1 [Reporter]Cy3 lights (CtrlF24)_PCR/505 control_unknown_type 1 3 18 1 [Reporter]P6 (CtrlE18)_PCR/4406 4 2 22 5 [Reporter]no spot (18D7)_PCR/3994 4 2 22 2 [Reporter]50% DMSO (CtrlD3)_PCR/3991 4 2 22 1 [Reporter]Cy3 lights carry-over (CtrlL23)_PCR/3990 control_unknown_type 4 2 21 1 [Reporter]P10 (CtrlD23)_PCR/3968 4 2 20 14 [Reporter]no spot (9P11)_PCR/3959 4 2 20 1 [Reporter]P8 (CtrlP19)_PCR/3946 4 2 19 11 [Reporter]no spot (12P7)_PCR/3934 4 2 19 1 [Reporter]P7 (CtrlL19)_PCR/3924 4 2 18 4 [Reporter]no spot (18P23)_PCR/3905 4 2 18 1 [Reporter]P6 (CtrlH19)_PCR/3902 1 1 23 9 [Reporter]no spot (14E12)_PCR/491 1 1 23 5 [Reporter]no spot (18E8)_PCR/487 1 1 23 1 [Reporter]Cy5 lights (CtrlM24)_PCR/484 control_unknown_type 1 3 17 1 [Reporter]P5 (CtrlA18)_PCR/4384 1 1 22 2 [Reporter]50% DMSO (CtrlA4)_PCR/463 1 1 22 1 [Reporter]Cy3 lights carry-over (CtrlI24)_PCR/462 control_unknown_type 1 3 16 1 [Reporter]P4 (CtrlM14)_PCR/4362 1 1 21 10 [Reporter]no spot (13A12)_PCR/449 1 1 21 1 [Reporter]P10 (CtrlA24)_PCR/440 1 3 15 1 [Reporter]P3 (CtrlI14)_PCR/4340 1 1 20 2 [Reporter]no spot (20I24)_PCR/419 1 1 20 1 [Reporter]P8 (CtrlM20)_PCR/418 1 3 14 12 [Reporter]no spot (11M2)_PCR/4329 1 1 19 5 [Reporter]no spot (18E4)_PCR/400 1 3 14 1 [Reporter]P1 (CtrlE14)_PCR/4318 4 2 17 10 [Reporter]no spot (13D7)_PCR/3889 4 2 17 1 [Reporter]P5 (CtrlD19)_PCR/3880 4 2 16 1 [Reporter]P4 (CtrlP15)_PCR/3858 4 2 15 13 [Reporter]no spot (10H7)_PCR/3848 4 2 15 1 [Reporter]P3 (CtrlL15)_PCR/3836 4 2 14 8 [Reporter]no spot (14P23)_PCR/3821 4 2 14 1 [Reporter]P1 (CtrlH15)_PCR/3814 1 1 5 12 [Reporter]no spot (10I20)_PCR/99 1 1 5 2 [Reporter]no spot (20M8)_PCR/89 1 1 5 1 [Reporter]16s rRNA 1:16 (CtrlM4)_PCR/88 control_biosequence 1 1 4 1 [Reporter]16s rRNA 1:4 (CtrlA8)_PCR/66 control_biosequence 1 1 3 7 [Reporter]no spot (15M12)_PCR/50 1 1 19 1 [Reporter]P7 (CtrlI20)_PCR/396 1 1 3 1 [Reporter]16s rRNA 1:1 (CtrlE8)_PCR/44 control_biosequence 1 3 13 1 [Reporter]23s rRNA 1:256 (CtrlM6)_PCR/4296 control_biosequence 1 1 18 1 [Reporter]P6 (CtrlE20)_PCR/374 1 1 2 1 [Reporter]16s carry-over (CtrlI8)_PCR/22 1 3 12 16 [Reporter]no spot (7E6)_PCR/4289 1 3 12 1 [Reporter]23s rRNA 1:64 (CtrlA10)_PCR/4274 control_biosequence 1 1 17 5 [Reporter]no spot (17M24)_PCR/356 1 1 17 1 [Reporter]P5 (CtrlA20)_PCR/352 1 3 11 1 [Reporter]23s rRNA 1:16 (CtrlE10)_PCR/4252 control_biosequence 1 1 16 1 [Reporter]P4 (CtrlM16)_PCR/330 1 1 15 18 [Reporter]no spot (5I12)_PCR/325 1 3 10 4 [Reporter]no spot (18M14)_PCR/4233 1 3 10 1 [Reporter]23s rRNA 1:4 (CtrlI10)_PCR/4230 control_biosequence 1 3 9 21 [Reporter]no spot (2M6)_PCR/4228 1 1 15 1 [Reporter]P3 (CtrlI16)_PCR/308 1 3 9 13 [Reporter]no spot (9M22)_PCR/4220 1 1 14 18 [Reporter]no spot (5E12)_PCR/303 1 3 9 1 [Reporter]23s rRNA 1:1 (CtrlM10)_PCR/4208 control_biosequence 4 2 13 5 [Reporter]no spot (17P19)_PCR/3796 4 2 13 1 [Reporter]23s rRNA 1:256 (CtrlP7)_PCR/3792 control_biosequence 4 2 12 18 [Reporter]no spot (5P7)_PCR/3787 4 2 12 11 [Reporter]no spot (12D3)_PCR/3780 4 2 12 1 [Reporter]23s rRNA 1:64 (CtrlD11)_PCR/3770 control_biosequence 4 2 11 15 [Reporter]no spot (8P3)_PCR/3762 4 2 11 1 [Reporter]23s rRNA 1:16 (CtrlH11)_PCR/3748 control_biosequence 4 2 10 1 [Reporter]23s rRNA 1:4 (CtrlL11)_PCR/3726 control_biosequence 4 2 9 6 [Reporter]no spot (16D19)_PCR/3709 4 2 9 2 [Reporter]no spot (20P11)_PCR/3705 4 2 9 1 [Reporter]23s rRNA 1:1 (CtrlP11)_PCR/3704 control_biosequence 4 2 8 20 [Reporter]no spot (3H7)_PCR/3701 1 1 14 1 [Reporter]P1 (CtrlE16)_PCR/286 1 3 8 1 [Reporter]23s carry-over (CtrlA14)_PCR/4186 1 1 13 1 [Reporter]23s rRNA 1:256 (CtrlM8)_PCR/264 control_biosequence 1 3 7 14 [Reporter]no spot (8I22)_PCR/4177 1 3 7 1 [Reporter]16s rRNA 1:256 (CtrlE2)_PCR/4164 control_biosequence 1 1 12 1 [Reporter]23s rRNA 1:64 (CtrlA12)_PCR/242 control_biosequence 1 1 11 19 [Reporter]no spot (4M8)_PCR/238 1 1 11 16 [Reporter]no spot (7A8)_PCR/235 1 3 6 1 [Reporter]16s rRNA 1:64 (CtrlI2)_PCR/4142 control_biosequence 1 1 11 4 [Reporter]no spot (18A20)_PCR/223 1 1 11 1 [Reporter]23s rRNA 1:16 (CtrlE12)_PCR/220 control_biosequence 1 3 5 10 [Reporter]no spot (12A18)_PCR/4129 1 3 5 1 [Reporter]16s rRNA 1:16 (CtrlM2)_PCR/4120 control_biosequence 4 2 8 7 [Reporter]no spot (15D19)_PCR/3688 4 2 8 1 [Reporter]23s carry-over (CtrlD15)_PCR/3682 4 2 7 20 [Reporter]no spot (3D7)_PCR/3679 4 2 7 1 [Reporter]16s rRNA 1:256 (CtrlH3)_PCR/3660 control_biosequence 4 2 6 14 [Reporter]no spot (8H23)_PCR/3651 4 2 6 1 [Reporter]16s rRNA 1:64 (CtrlL3)_PCR/3638 control_biosequence 1 1 1 6 [Reporter]no spot (16A12)_PCR/5 1 1 1 1 [Reporter]Cy3 lights (CtrlE24)_PCR/1 control_unknown_type 4 2 5 1 [Reporter]16s rRNA 1:16 (CtrlP3)_PCR/3616 control_biosequence