Array Design Name Incyte Drosophila LifeArray v1.0
Provider Brian Oliver(Brian_Oliver@nih.gov)
Comment[ArrayExpressAccession] A-GEOD-20
Comment[SecondaryAccession] GPL20
Comment[Description] Distribution: non-commercial, Technology: spotted DNA/cDNA, The array was assembled using amplicons representing approximately 93% of the genes predicted in version 1.0 of the Drosophila genome from sequences released by the Berkeley Drosophila Genome Project and Celera Genomics in March 2000. The amplicons represent approximately 75% of the genes predicted in the January 2003 release 3.1 Drosophila genome annotation update. Incyte in-house algorithms were used to develop gene-specific primers averaging 20 nucleotides in length that were used to PCR amplify one exon or exon fragment from every predicted gene in the fly genome. The regions amplified to build the array ranged in size from 150 to 600 bp with an average size of 410 bp. PCR fragments were amplified and each amplicon was quality control tested for DNA concentration, purity, and fragment size. A total of 13,856 unique Drosophila elements are included on the array. Each element was spotted in duplicate(some are spotted multiple times) for a total of 29,222 elements which were distributed on two separate glass slides designated subarrays A and B. In addition to the Drosophila elements, both halves of the array contain non-Drosophila controls elements that serve to orient the arrays, function to correct for background and to provide calibration standards. The total number of elements (Drosophila transcripts and controls) is 31,464. Each array element includes an area of 150 micron diameter with a center to center separation of 170 microns. Adhesion to glass was facilitated by UV crosslinking at 120,000 mj/cm2. Qualification of the arrays was examined by dying representatives of each print run with Syto 61 dye. Dyed slides were read with a GenePix 4000A scanner (Axon Instruments, Foster City, CA) at 535 nm. The April 2007 platform update is based on the Dmel_RELEASE 4.1 re-annotation result from DGRC (https://dgrc.cgb.indiana.edu/microarrays/annotation.html).
Comment[FLYBASE] = Flybase ID assigned by DGRC remapping
Comment[FLYBASE_SECONDARY] = Secondary Flybase ID assigned by DGRC remapping
Comment[RELEASE] = D.mel annotation version of DGRC remapping
Comment[GENE_NAME] = Dmel_RELEASE 4.1 Gene name assigned by DGRC remapping
Comment[GENE_SYMBOL] = Dmel_RELEASE 4.1 Gene symbol assigned by DGRC remapping
Comment[GENE_SYNONYMS] = Dmel_RELEASE 4.1 Gene synonyms assigned by DGRC remapping
Comment[DGRC.PROBE_ID] = Probe ID assigned by DGRC
Comment[CHROMOSOME] = Dmel_RELEASE 4.1 Chromsome location
Comment[TRANSCRIPTS] = Dmel_RELEASE 4.1 Transcript assigned by DGRC remapping
Comment[START_COORD] = Dmel_RELEASE 4.1 Start coordinate of predicted amplicon
Comment[STOP_COORD] = Dmel_RELEASE 4.1 Stop coordinate of amplicon
Comment[LENGTH] = Amplicon length
Comment[CYTO] = Dmel_RELEASE 4.1 Cytological location
Comment[GO_MOL_FUNC] = GO assignment, Molecular Function
Comment[GO_BIO_PROC] = GO assignment, Biological Process
Comment[GO_CELL_COMP] = GO assignment, Cellular Component
Comment[R3.1_GENE_NAME] = Gene name in Dmel_RELEASE 3.1 mapping
Comment[R1_GENE_NAME] = Gene name in Dmel_RELEASE 1 mapping
Comment[SUBARRAY] = Each array includes elements split into two chips designated A or B
Comment[LOCATION] = Location of element on the subarray
Comment[PLATEID] = Plate ID for amplicon source used for array elements
Comment[PLATEROW] = Row location of amplicons on plate
Comment[PLATECOLUMN] = Column location of amplicons on plate
Comment[INCYTECLONEID] = Incyte clone ID number used for array
Comment[CLONESOURCE] = Source of clone
Comment[PRIMERS] = Sequence of primers used for PCR amplification
Comment[STATUS] = 1 - PCR status, PCR products flagged by Incyte quality control that showed failed amplification, multiple bands, wrong size bands or primer problems, 2 - predicted amplicon not from dmelr3 or mitochondrial genome sequence, but heterochromatin scaffolds, 3 - multiple amplicons predicted, 4 - multiple BLAST hits for amplicon(s), 5 - amplicon(s) map to multiple genome features, 6 - FLAGGED_PROBLEMATIC by Flybase in D.mel annotations
Reporter Database Entry [clone_id] = Flybase ID assigned by DGRC remapping LINK_PRE:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=nucleotide&cmd=search&term=
Comment[SPOT_ID] = identifies spots without FBgn number in CLONE_ID
Comment[SubmittedName] Incyte Drosophila LifeArray v1.0
Comment[Organism] Drosophila melanogaster
Printing Protocol
Surface Type
Substrate Type
Term Source Name clone_id
Comment[AdditionalFile:txt] A-GEOD-20_comments.txt
[main]
Reporter Name Reporter Database Entry [clone_id]
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2014 FBgn0038043
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2019 FBgn0053087
2020 FBgn0004780
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19843
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22012
22013 FBgn0029898
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22146 FBgn0031908
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22154 FBgn0002031
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22164 FBgn0032275
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22227 FBgn0033067
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22277 FBgn0002921
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30659
30660
30661
30662
30663
30664
30665
30666
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30701
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30711
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30824
30825
30826
30827
30828
30829
30830
30831
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30989
30990
30991
30992
30993
30994
30995
30996
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31058
31059
31060
31061
31062
31063
31064
31065
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31109
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31127
31128
31129
31130
31131
31132
31133
31134
31135 FBgn0061515
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31169
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31179
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31207
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31276
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31292
31293
31294
31295
31296
31297
31298
31299
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31456
31457
31458
31459
31460
31461
31462
31463
31464