Array Design Name NIA MGC, Mammalian Genome Collection
Provider Kevin G Becker(beckerk@grc.nia.nih.gov)
Comment[ArrayExpressAccession] A-GEOD-1211
Comment[SecondaryAccession] GPL1211
Comment[Description] Array Manufacturer: NIA Gene Expression and Genomics Unit, Distribution: non-commercial, Technology: spotted DNA/cDNA, The goal of the Mammalian Gene Collection (MGC), a trans-NIH initiative, is to provide full-length open reading frame (FL-ORF) clones for human, mouse, and rat genes. In 2005, the project added the cow cDNAs generated by Genome Canada. Initially, cDNA libraries provided the source of the clones. Recently, alternative methods based on gene-specific amplification have been developed to target the recovery of human and mouse genes absent from the MGC collection.
Reporter Database Entry [genbank] = GeneBank accession number LINK_PRE:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=Nucleotide&term=
Reporter Database Entry [clone_id] = Image Clone ID
Comment[GENE_SYMBOL] = Gene symbol
Comment[GENE_NAME] = Descriptive gene name
Comment[SPOT_ID] = Indicator of blank positions on array
Comment[SubmittedName] NIA MGC, Mammalian Genome Collection
Comment[Organism] Homo sapiens
Printing Protocol PCR amplified cDNA of ~16,800 individual clones from the NIH Mammalian Genome Collection with sucrose cresol dye for visualization were denatured with NaOH and spotted onto S&S Nytran SuperCharge nylon membranes ~12cm X 8cm. Twenty-four 384 well plates are printed using a BioRobotics MicroGrid II Arrayer with a 384 pin head. Each of the 384 sub-arrays is a 5X5 grid of 24 spots and 1 blank.
Surface Type
Substrate Type S&S Nytran SuperCharge Nylon
Term Source Name clone_id genbank
Comment[AdditionalFile:txt] A-GEOD-1211_comments.txt
[main]
Reporter Name Reporter Database Entry [genbank] Reporter Database Entry [clone_id]
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1350
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1375
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1377 IMAGE:4155481
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1400
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1425
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1427 IMAGE:4155403
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1450
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1475
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1477 IMAGE:4155381
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1500
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1525
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1550
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1575
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1600
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1602 IMAGE:4155347
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1625
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1627 IMAGE:3464195
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1647 IMAGE:4300206
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1650
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1652 IMAGE:4155340
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1675
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1700
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1702 IMAGE:4155335
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1725
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1727 IMAGE:4155313
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1750
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1752 IMAGE:4155323
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1775
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1777 IMAGE:4155318
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1790 IMAGE:4131853
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1800
1801 BC000907 IMAGE:2984856
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1825
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1838 IMAGE:2905394
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1850
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1875
1876 BC001001 IMAGE:3048733
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1900
1901 BC000908 IMAGE:2985046
1902 BC009017 IMAGE:4157026
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1911 BC002637 IMAGE:3607549
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1925
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1950
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