Array Design Name	R & D Human Cytokine Array
Provider	R A P M Perera(h0696905@hkusua.hku.hk)
Comment[ArrayExpressAccession]	A-GEOD-10327
Comment[SecondaryAccession]	GPL10327
Comment[Description]	Array Manufacturer: R&D Systems, Distribution: custom-commercial, Technology: spotted protein<br>Comment[ORF] = Symbol LINK_PRE:http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term= LINK_SUF:[gene name] AND Homo sapiens[organism]<br>Comment[Entrez Gene] = (LocusLink)/GenBank/GenPept/GI Number/HUGO Gene Symbol/Illumina/International Protein Index/KEGG/miRBase (mature)/PubChem CID/RefSeq/UniGene/UniProt/Swiss-Prot Accession(A3)
Comment[SubmittedName]	R & D Human Cytokine Array
Comment[Organism]	Homo sapiens
Printing Protocol	Proteome Profiler 96 Microplate-based Antibody Arrays consist of a 96-well microplate pre-spotted with multiple capture antibodies in each well. Experimental samples are added and target proteins present in the samples bind to the immobilized antibodies. All wells also contain Reference Spots, which provide a strong positive signal to allow easy visualization of well location. A cocktail of biotinylated detection antibodies and Streptavidin-HRP or an HRP-conjugated pan anti-phospho-tyrosine antibody is subsequently used to detect the bound proteins. Chemiluminescent substrate reagents produce a signal that is proportional to the amount of analyte bound. A compatible digital imaging system capable of chemiluminescent detection is used to determine the intensity of light emitted from individual spots.
Surface Type
Substrate Type
Term Source Name
Comment[AdditionalFile:txt]	A-GEOD-10327_comments.txt

[main]
Reporter Name
ADAM8
ADAMTS5
AMPD3 (includes EG:272)
BDKRB1
CCL3
CCL4
CCL5
CCL13
CCR2
CD40LG
CMA1
COPS5
CSF2
CSF3
CXCL1
CXCL10
CXCL11
CXCL12
CXCR3
G6PD
GPR132
HAS1
ICAM1
IFNB1
IFNG
IL2
IL4
IL5
IL6
IL8
IL10
IL13
IL16
IL25
IL27
IL29
IL32A
IL12A
IL17A (includes EG:3605)
IL1A
IL1B
IL1R1
IL1R2
IL1RN
IL23A
IL8RB
INHBB
LPA
LPAR1
LPAR2
LPAR3
MCAM
MIF
MYBPC1
MYBPC3
NR4A3
PLA2G4A
PPP1R12B
PTX3
RNASE1
SERPINE1
SFTPA1B
TLR1
TNF
TNFAIP6
TPSD1
TREM1
VDR