E-TIGR-5001 - Transcription profiling of Geobacter sulfurreducens grown in batch culture with either ferric citrate or graphite anodes as the electron acceptor
Released on 2 July 2007, last updated on 2 May 2014
G. sulfurreducens (ATCC #51573) was obtained from the laboratory culture collection of Dr. Derek Lovley. Cells were grown under strict anaerobic conditions at 23 °C in batch culture. Both control and experimental populations were grown with 10mM acetate as the electron donor while Fe(III) as 55 mM ferric citrate served as the electron acceptor for the control populations and graphite anodes as the electron acceptor for the experimental populations. Controls populations were harvested at a concentration of 30 mM Fe(II). Fuel cell power output was monitored by measuring the voltage across a known resistance in the fuel cell and experimental populations were harvested when current production reached a steady state of 0.6 milliamperes. Cells were harvested at log phase by centrifugation at 4 °C and the cell pellet was flash-frozen in liquid nitrogen and then stored at -80 °C prior to RNA extraction. Three cultures of control and experimental populations were collected. Each culture vessel was harvested and extracted for total RNA separately to produce three biological replicates for this experiment. Additional information regarding the fuel cell experimental set up and culture conditions for this experiment can be found in both the current reference and the following reference: Bond DR and Lovley DR. Electricity production by Geobacter sulfurreducens attached to electrodes. 2003. Applied and Environmental Microbiology 69: 1548-1555.
transcription profiling by array, compound treatment, dye swap