E-TABM-877 - Transcriptional profiling of mouse pancreatic beta-cells, embryonic stem cells and 10 other normal adult tissues
Submitted on 26 January 2010, released on 19 March 2010, last updated on 2 May 2014
To gain insights into how pancreatic beta-cells are programmed in vivo, we profiled key histone methylations (H3K4/K27me3) in embryonic stem cells, multipotent progenitors of the nascent embryonic pancreas, purified beta-cells, and 10 other adult tissues (all under normal, untreated conditions). For these cells we also purified RNA to analyze tissue specfic genome wide transcription levels in relation to histone modifications.
transcription profiling by array, co-expression, in vivo, organism part comparison
Derepression of Polycomb targets during pancreatic organogenesis allows insulin-producing beta-cells to adopt a neural gene activity program. van Arensbergen, Joris; García-Hurtado, Javier; Moran, Ignasi; Maestro, Miguel Angel; Xu, Xiaobo; Van de Casteele, Mark; Skoudy, Anouchka L.; Palassini, Matteo; Heimberg, Harry; Ferrer, Jorge. Genome Res 20(6):722 (2010), PMID:20395405