E-TABM-865 - Transcription profiling of mouse liver and spleen from strains that are susceptible and tolerant to trypanosome infection
Released on 2 December 2009, last updated on 29 October 2015
Tissues were harvested from mice from two congenic strains (Tir1CC and Tir3CC) and their two control strains (Tir1AA and Tir3AA). The congenic strains were bred from inbred lines known to be susceptible (A/J) and tolerant (C57BL/6) to Trypanosome infection, such that C57BL/6 genomic regions underlying QTL for trypanotolerance were present in an A/J genetic background. Liver and spleen tissues were harvested. For each strain RNA was prepared from various tissues of twenty mice. For each condition, RNA extracts from individuals were pooled (5 per pool) prior to hybridisation.
transcription profiling by array, co-expression, organism part comparison, strain or line
Genotype and expression analysis of two inbred mouse strains and two derived congenic strains suggest that most gene expression is trans regulated and sensitive to genetic background. Noyes HA, Agaba M, Anderson S, Archibald AL, Brass A, Gibson J, Hall L, Hulme H, Oh SJ, Kemp S. , Europe PMC 20529291