E-TABM-783 - Transcription profiling of human non anaplastic T-cell lymphoma

Submitted on 2 September 2009, released on 22 September 2009, last updated on 2 May 2014
Homo sapiens
Samples (35)
Array (1)
Protocols (6)
A ムCartes dメIdentite des Tumeursメ (CIT) project from the french Ligue Nationale Contre le Cancer (http://cit.ligue-cancer.net) | Affymetrix HG-U133 Plus 2.0 : 17 AITL biopsies, 2 AITL sorted cells, 16 PTCL NOS biopsies | The molecular alterations underlying the pathogenesis of angioimmunoblastic T-cell lymphoma (AITL) and peripheral T-cell lymphoma, unspecified (PTCL-u) are largely unknown. In order to characterize the ontogeny and molecular differences between both entities, a series of AITLs (n = 18) and PTCLs-u (n = 16) was analyzed using gene expression profiling. Unsupervised clustering correlated with the pathological classification and with CD30 expression in PTCL-u. The molecular profile of AITLs was characterized by a strong microenvironment imprint (overexpression of B-cell- and follicular dendritic cell-related genes, chemokines, and genes related to extracellular matrix and vascular biology), and overexpression of several genes characteristic of normal follicular helper T (T(FH)) cells (CXCL13, BCL6, PDCD1, CD40L, NFATC1). By gene set enrichment analysis, the AITL molecular signature was significantly enriched in published T(FH)-specific genes. The enrichment was higher for sorted AITL cells than for tissue samples. Overexpression of several T(FH) genes was validated by immunohistochemistry in AITLs. A few cases with molecular T(FH)-like features were identified among CD30(-) PTCLs-u. Our findings strongly support that T(FH) cells represent the normal counterpart of AITL, and suggest that the AITL spectrum may be wider than suspected, as a subset of CD30(-) PTCLs-u may derive from or be related to AITL.| Submitter : Aurelien de Reynies <reyniesa@ligue-cancer.fr> | Project leader : Philippe Gaulard <philippe.gaulard@hmn.aphp.fr>
Experiment types
transcription profiling by array, co-expression
The gene expression profile of nodal peripheral T-cell lymphoma demonstrates a molecular link between angioimmunoblastic T-cell lymphoma (AITL) and follicular helper T (TFH) cells. Laurence de Leval, David S. Rickman, Caroline Thielen, Aurelien de Reynies, Yen-Lin Huang, Georges Delsol, Laurence Lamant, Karen Leroy, Josette Briere, Thierry Molina, Fran�oise Berger, Christian Gisselbrecht, Luc Xerri, and Philippe Gaulard. Blood 109(11):4952-4963 (2007)
Investigation descriptionE-TABM-783.idf.txt
Sample and data relationshipE-TABM-783.sdrf.txt
Raw data (2)E-TABM-783.raw.1.zip, E-TABM-783.raw.2.zip
Processed data (1)E-TABM-783.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-TABM-783.eSet.r