E-TABM-672 - Transcription profiling of mouse embyronic stem cell line CGR8 during the first 10 days of differentiation into embryoid bodies

Status
Released on 10 July 2009, last updated on 29 October 2015
Organism
Mus musculus
Samples (27)
Array (1)
Protocols (5)
Description
This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th Framework Programme of the European Union, http://www.fungenes.org). The experiment was conducted at University of Cologne, Cologne, Germany. Goal of the experiment was RNA expression profiling in the first 10 days of differentiation in CGR8 cells. Materials and methods: CGR8 cells were cultivated as EBs without addition of selective reagents in hanging drop cultures in a timecourse experiment. After 7 days, EBs were plated in gelatin-coated 6-well plates. RNA was prepared from ES cells (day 0), EBs (day 1 to 7) and plated EBs (day 10). Culture conditions: ES cells were differentiated via hanging drop protocol into embryoid bodies. RNA isolation method: The RNA isolation was carried out following standard methods (on column, RNeasy Mini kit, Qiagen)
Experiment types
transcription profiling by array, co-expression, in vitro, time series
Contact
Citations
The FunGenES database: a genomics resource for mouse embryonic stem cell differentiation. Schulz H, Kolde R, Adler P, Aksoy I, Anastassiadis K, Bader M, Billon N, Boeuf H, Bourillot PY, Buchholz F, Dani C, Doss MX, Forrester L, Gitton M, Henrique D, Hescheler J, Himmelbauer H, Hübner N, Karantzali E, Kretsovali A, Lubitz S, Pradier L, Rai M, Reimand J, Rolletschek A, Sachinidis A, Savatier P, Stewart F, Storm MP, Trouillas M, Vilo J, Welham MJ, Winkler J, Wobus AM, Hatzopoulos AK, Functional Genomics in Embryonic Stem Cells Consortium. PLoS One 4(9):e6804 (2009), PMID:19727443
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-TABM-672.idf.txt
Sample and data relationshipE-TABM-672.sdrf.txt
Raw data (2)E-TABM-672.raw.1.zip, E-TABM-672.raw.2.zip
Array designA-AFFY-45.adf.txt
Links