E-TABM-631 - Transcription profiling time series of human estrogen-responsive human breast cancer cells treated with 17 beta-estradiol
Submitted on 29 January 2009, released on 3 February 2009, last updated on 2 May 2014
Human estrogen-responsive breast cancer cell line ZR-75-1 was treated with 10nM of 17 beta-estradiol. Gene expression was analyzed on total RNA extracted before or after 1, 2, 4, 6, 8, 12, 16, 20, 24, 28 and 32 hours hormonal stimulation. For each condition 2 technical replicates were performed, except the reference sample (before stimulation - 0h) which was in quadruplicate and the 4h sample in triplicate.
transcription profiling by array, co-expression, time series
Time-course analysis of genome-wide gene expression data from hormone-responsive human breast cancer cells. Mutarelli M, Cicatiello L, Ferraro L, Grober OM, Ravo M, Facchiano AM, Angelini C, Weisz A. BMC Bioinformatics 9:Suppl 2:S12 (2008)