E-TABM-517 - Transcription profiling of fission yeast strains designed to make swi5-based mapping more efficient and powerful
Released on 4 July 2008, last updated on 10 June 2011
Fission yeast genes identified in genetic screens are usually cloned by transformation of mutants with plasmid libraries. However, for some genes this can be difficult, and positional cloning approaches are required. The mutation swi5-39 reduces recombination frequency in homozygous crosses and has been used as a tool in mapping gene position (H. Schmidt, Curr. Genet (1993) 24:271-273). However, strain construction in swi5-39-based mapping is significantly more laborious than is desirable. Here we describe a set of strains designed to make swi5-based mapping more efficient and more powerful.
transcription profiling by array, co-expression, genetic modification
Improved tools for efficient mapping of fission yeast genes: microtubule nucleation modifier mutant mod22 linked to chromatin-remodeling factor gene swr1. Andreas Anders, Stephen Watt, Jürg Bähler, and Kenneth E. Sawin.