E-TABM-236 - Transcription profiling of Strongyloides ratti free living vs parasitic females

Status
Submitted on 22 February 2007, released on 24 May 2008, last updated on 2 May 2014
Organism
Strongyloides ratti
Samples (6)
Arrays (2)
Protocols (12)
Description
In previous studies we have shown that the two adult females morphs of S. ratti have very different lifespans. This experiment was designed to try to identify differentially expressed genes in these two adult morphs that may account for these differing lifespans. The genes expressed by S. ratti parasitic females at day 6 p.i. were compared to the genes expressed by S. ratti free living females at 3 days 19 degrees C. This comparison was done using a microarray chip that is spotted with PCR fragments from the libraries that were generated from parasitic females extracted at day 6 and day 15 p.i., and a microarray chip that is spotted with PCR fragments from the libraries that were generated from free-living larval stages L1, L2 and infective L3s and from free-living males and females.
Experiment types
transcription profiling by array, growth condition
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-TABM-236.idf.txt
Sample and data relationshipE-TABM-236.sdrf.txt
Raw data (1)E-TABM-236.raw.1.zip
Processed data (1)E-TABM-236.processed.1.zip
Array designsA-MEXP-334.adf.txt, A-MEXP-650.adf.txt
R ExpressionSetE-TABM-236.eSet.r