E-TABM-222 - Translation profiling of Schizosaccharomyces pombe size separated polysome fractions provides data on average numbers of associated ribosomes for most transcripts
Submitted on 5 February 2007, released on 22 March 2007, last updated on 4 June 2014
To obtain translational profiles for all mRNAs, polysome preparations are separated according to their size using a sucrose gradient and the mRNAs in each fraction are identified and quantified with DNA microarrays. Starting with exponentially growing cells, we analyzed 12 polysome fractions using DNA microarrays containing elements for all known and predicted genes of fission yeast. This approach provided data on average numbers of associated ribosomes for most transcripts.
translation profiling, co-expression, reference, translational bias
A Network of Multiple Regulatory Layers Shapes Gene Expression in Fission Yeast. Daniel H. Lackner, Traude H. Beilharz, Samuel Marguerat, Juan Mata, Stephen Watt, Falk Schubert, Thomas Preiss and Jï¿½rg Bï¿½hler. Mol Cell in review