E-TABM-217 - Poly(A) tail length profiling of mRNAs from fission yeast
Submitted on 5 February 2007, released on 16 May 2007, last updated on 2 May 2014
To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarrays using total eluate as a reference.
other, co-expression, translational bias
A Network of Multiple Regulatory Layers Shapes Gene Expression in Fission Yeast. Daniel H. Lackner, Traude H. Beilharz, Samuel Marguerat, Juan Mata, Stephen Watt, Falk Schubert, Thomas Preiss and Jï¿½rg Bï¿½hler. Mol Cell in review