E-TABM-162 - Transcription profiling of D. melanogaster Twist homozygous mutants vs. wildtype embryos at various developmental stages
Submitted on 9 October 2006, released on 28 March 2007, last updated on 2 May 2014
Expression profiling of Drosophila twist homozygous mutant embryos in a timecourse of embryogenesis. Four one-hour timepoints were assayed (4-8 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together to cDNA arrays.
transcription profiling by array, development or differentiation, dye swap, genetic modification, time series, transcript identification
A core transcriptional network for early mesoderm development in Drosophila melanogaster. Sandmann T, Girardot C, Brehme M, Tongprasit W, Stolc V, Furlong EE. Genes Dev 21(4):436-49 (2007), Europe PMC 17322403