E-TABM-127 - Transcription profiling by array of human bronchial epithelial cells after exposure to cigarette smoke
Released on 15 January 2007, last updated on 24 July 2015
Cell cultures from three human non-smoker donors were exposed 1:50 dose (1.84ug/cm2) of diluted mainstream cigarette smoke or filtered air for 1h. Cells from four replicate inserts for each dose/time-point were pooled and placed in TRIzol either immediately after exposure or following a 5h or 23h recovery period after the cultures were returned to an incubator at 37¬∫C, 5% CO2. The ¬ëno treatment¬í control (NTC) remained in the incubator and received neither smoke nor air. In order to perform robust statistical analyses three independent replicate experiments were performed on each of the three donors
transcription profiling by array, stimulus or stress, time series
Human bronchial epithelial cell transcriptome: gene expression changes following acute exposure to whole cigarette smoke in vitro. Maunders H, Patwardhan S, Phillips J, Clack A, Richter A.