E-TABM-127 - Transcription profiling by array of human bronchial epithelial cells after exposure to cigarette smoke

Status
Released on 15 January 2007, last updated on 24 July 2015
Organism
Homo sapiens
Samples (63)
Array (1)
Protocols (12)
Description
Cell cultures from three human non-smoker donors were exposed 1:50 dose (1.84ug/cm2) of diluted mainstream cigarette smoke or filtered air for 1h. Cells from four replicate inserts for each dose/time-point were pooled and placed in TRIzol either immediately after exposure or following a 5h or 23h recovery period after the cultures were returned to an incubator at 37ºC, 5% CO2. The ‘no treatment’ control (NTC) remained in the incubator and received neither smoke nor air. In order to perform robust statistical analyses three independent replicate experiments were performed on each of the three donors
Experiment types
transcription profiling by array, stimulus or stress, time series
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-TABM-127.idf.txt
Sample and data relationshipE-TABM-127.sdrf.txt
Raw data (2)E-TABM-127.raw.1.zip, E-TABM-127.raw.2.zip
Processed data (1)E-TABM-127.processed.1.zip
Array designA-AFFY-44.adf.txt
Links