E-SMDB-3596 - Transcription profiling of C. elegans wild type L2 or L3 larvae (non-dauer) vs TGFbeta mutants at similar developmental stages undergoing dauer formation

Status
Released on 6 January 2006, last updated on 4 June 2014
Organism
Caenorhabditis elegans
Samples (0)
Arrays (4)
Protocols (2)
Description
BACKGROUND: When resources are scant, C. elegans larvae arrest as long-lived dauers under the control of insulin/IGF- and TGFbeta-related signaling pathways. RESULTS: We have identified genes that show different levels of expression in a comparison of wild-type L2 or L3 larvae (non-dauer) to TGFbeta mutants at similar developmental stages undergoing dauer formation. Many insulin/IGF pathway and other known dauer regulatory genes have changes in expression that suggest strong positive feedback by the TGFbeta pathway. In addition, many insulin-like ligand and novel genes with similarity to the extracellular domain of insulin/IGF receptors have altered expression. We have identified a large group of regulated genes with putative binding sites for the FOXO transcription factor, DAF-16. Genes with DAF-16 sites upstream of the transcription start site tend to be upregulated, whereas genes with DAF-16 sites downstream of the coding region tend to be downregulated. Finally, we also see strong regulation of many novel hedgehog- and patched-related genes, hormone biosynthetic genes, cell cycle genes, and other regulatory genes.
Experiment types
transcription profiling by array, unknown experiment type
Contacts
Citation
MIAME
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