E-SMDB-3539 - Transcription profiling of C. elegans N2 worms treated with Resveratrol
Submitted on 7 November 2005, released on 7 November 2005, last updated on 27 March 2012
Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using Trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization.
transcription profiling by array, compound treatment
A Role for SIR-2.1 Regulation of ER Stress Response Genes in Determining C. elegans Life Span. Viswanathan M, Kim SK, Berdichevsky A, Guarente L. Dev Cell 9(5):605-15 (2005), Europe PMC 16256736