E-SMDB-3539 - Transcription profiling of C. elegans N2 worms treated with Resveratrol

Status
Submitted on 7 November 2005, released on 7 November 2005, last updated on 27 March 2012
Organism
Caenorhabditis elegans
Samples (0)
Array (1)
Protocols (4)
Description
Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using Trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization.
Experiment types
transcription profiling by array, compound treatment
Contacts
Citation
A Role for SIR-2.1 Regulation of ER Stress Response Genes in Determining C. elegans Life Span. Viswanathan M, Kim SK, Berdichevsky A, Guarente L. Dev Cell 9(5):605-15 (2005), Europe PMC 16256736
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-SMDB-3539.idf.txt
Sample and data relationshipE-SMDB-3539.sdrf.txt
Raw data (1)E-SMDB-3539.raw.1.zip
Processed data (1)E-SMDB-3539.processed.1.zip
Array designA-SMDB-981.adf.txt
Links