E-SMDB-3208 - Transcription profiling of Entamoeba histolytica plus Caco2 cells
Released on 6 July 2005, last updated on 27 March 2012
Entamoeba histolytica is a protozoan parasite which causes colitis and liver abscesses. Using a genomic DNA microarray consisting of 1.6 - 2.0 kb genomic inserts we have generated a transcriptional profile of 1,971 unique parasite transcripts. The arrays in this experiment set were used to examine changes in the transcriptional profile of Entamoeba histolytica when the parasite interacts with colonic epithelial cells (Caco-2). A time course was used such that RNA was isolated from ameba alone and ameba + Caco-2 cells at 3hrs, 6hrs and 9hrs corresponding to 10%, 50% and 90% cell monolayer destruction. At least two biological experiments and three replicates were used for each time point.
transcription profiling by array, time series
Transcriptional profiling of Entamoeba histolytica trophozoites. Macfarlane RC, Shah PH, Singh U. Int J Parasitol 35:533-42 (2005-04-30), Europe PMC 15826645