E-SMDB-3207 - Transcription profiling of Entamoeba histolytica trophozoites heat shock experiments

Status
Submitted on 6 July 2005, released on 6 July 2005, last updated on 27 March 2012
Organism
Entamoeba histolytica
Samples (0)
Array (1)
Protocols (4)
Description
Entamoeba histolytica is a protozoan parasite which causes colitis and liver abscesses. A pilot microarray consisting of 360 unique parasite genes was constructed using identical methods to the larger array (1,971 unique genes). The four arrays in this data set were used to ascertain whether the microarrays would be useful in detecting changes in transcript abundance by exposing parasites to heat shock (42 0C for 1 hr). Approximately, 17% of the genes were regulated by at least two fold including many genes previously shown to be involved in heat shock response. This data confirmed that the genomic DNA arrays were useful in detecting changes in transcript abundance.
Experiment types
transcription profiling by array, unknown experiment type
Contacts
Citation
Transcriptional profiling of Entamoeba histolytica trophozoites. Macfarlane RC, Shah PH, Singh U. Int J Parasitol 35:533-42 (2005-04-30), Europe PMC 15826645
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-SMDB-3207.idf.txt
Sample and data relationshipE-SMDB-3207.sdrf.txt
Raw data (1)E-SMDB-3207.raw.1.zip
Processed data (1)E-SMDB-3207.processed.1.zip
Array designA-SMDB-762.adf.txt
Links